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Disease Index for the Rust Puccinia psidii on Rose Apple in ... - ctahr

Disease Index for the Rust Puccinia psidii on Rose Apple in ... - ctahr

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Mak<strong>in</strong>g evaluati<strong>on</strong>s <strong>in</strong> <str<strong>on</strong>g>the</str<strong>on</strong>g> field<br />

• For all sites, record <str<strong>on</strong>g>the</str<strong>on</strong>g> host plant, locati<strong>on</strong>, date, and disease category (as<br />

described below). A survey <str<strong>on</strong>g>for</str<strong>on</strong>g>m is provided <strong>on</strong> page 11.<br />

• For locati<strong>on</strong>, GPS coord<strong>in</strong>ates are ideal. Lack<strong>in</strong>g a GPS device, if you are <strong>on</strong><br />

a trail, you can note which part you are <strong>on</strong> by estimat<strong>in</strong>g <str<strong>on</strong>g>the</str<strong>on</strong>g> distance traveled<br />

(first quarter of <str<strong>on</strong>g>the</str<strong>on</strong>g> trail, half way al<strong>on</strong>g, etc.), or you can note some land<br />

feature (near a certa<strong>in</strong> stream, ridge, etc.); if near a highway, give its name<br />

and nearest milepost marker if available.<br />

• Determ<strong>in</strong>e if <str<strong>on</strong>g>the</str<strong>on</strong>g> rust is present at a site by check<strong>in</strong>g all sides of at least five<br />

trees; if no rust is found, <str<strong>on</strong>g>the</str<strong>on</strong>g> site’s record is zero (absence of <str<strong>on</strong>g>the</str<strong>on</strong>g> rust at a site<br />

is useful <strong>in</strong><str<strong>on</strong>g>for</str<strong>on</strong>g>mati<strong>on</strong>). For tall trees, check <str<strong>on</strong>g>the</str<strong>on</strong>g> lower canopy and make a note<br />

of <str<strong>on</strong>g>the</str<strong>on</strong>g> tree size.<br />

• Place specimens <strong>in</strong> separate bags and wipe hands with a moist tissue be<str<strong>on</strong>g>for</str<strong>on</strong>g>e<br />

handl<strong>in</strong>g a new plant. Avoid touch<strong>in</strong>g healthy plants if you have handled<br />

plants with heavy <strong>in</strong>fecti<strong>on</strong> levels. Wip<strong>in</strong>g hands is recommended after each<br />

sampl<strong>in</strong>g. Although movement of <str<strong>on</strong>g>the</str<strong>on</strong>g> pathogen is largely by <str<strong>on</strong>g>the</str<strong>on</strong>g> w<strong>in</strong>d and ra<strong>in</strong><br />

splash, c<strong>on</strong>tact can also move spores.<br />

• In <str<strong>on</strong>g>the</str<strong>on</strong>g> field, evaluators should rate a site or tree us<strong>in</strong>g <str<strong>on</strong>g>the</str<strong>on</strong>g> highest category <str<strong>on</strong>g>for</str<strong>on</strong>g><br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> rat<strong>in</strong>g. When a tree is severely <strong>in</strong>fected and young stems are covered with<br />

spores, <str<strong>on</strong>g>the</str<strong>on</strong>g> disease is Category 4, even if <str<strong>on</strong>g>the</str<strong>on</strong>g> older leaves have <strong>on</strong>ly a few<br />

spots (Category 1 or 2). Note <str<strong>on</strong>g>the</str<strong>on</strong>g> category of <str<strong>on</strong>g>the</str<strong>on</strong>g> older leaves, buds, flowers,<br />

and fruits, if present. When a tree has <strong>on</strong>ly old growth with dead branch tips,<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> disease is Category 5, but you should also make a note if <str<strong>on</strong>g>the</str<strong>on</strong>g>re are o<str<strong>on</strong>g>the</str<strong>on</strong>g>r<br />

leaves <strong>in</strong> Category 1 to 3, and check <str<strong>on</strong>g>for</str<strong>on</strong>g> any o<str<strong>on</strong>g>the</str<strong>on</strong>g>r plant parts, such as<br />

flowers, that may be diseased.<br />

4

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