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(12) PATENT APPLICATION PUBLICATION (21) Application No.6848/DELNP/2008 A<br />

(19) INDIA<br />

(22) Date <strong>of</strong> filing <strong>of</strong> Application :08/08/2008 (43) Publication Date : 24/10/2008<br />

(54) Title <strong>of</strong> the invention : "MONITORING ENZYME MIXTURES"<br />

(51) International classification :C12Q 1/37 (71)Name <strong>of</strong> Applicant :<br />

(31) Priority Document No :0602518.3<br />

1)ANALYTICA NANO TECHNOLOGIES LIMITED<br />

(32) Priority Date :08/02/2006<br />

Address <strong>of</strong> Applicant :NETPARK INCUBATOR, THOMAS<br />

(33) Name <strong>of</strong> priority country :U.K.<br />

WRIGHT WAY, NETPARK, SEDGEFIELD TS21 3FD, GREAT<br />

(86) International Application No :PCT/GB2007/000430 BRITAIN. U.K.<br />

Filing Date<br />

:08/02/2007 (72)Name <strong>of</strong> Inventor :<br />

(87) International Publication No :WO 2007/091065 1)ROWELL, FREDERICK, JOHN<br />

(61) Patent <strong>of</strong> Addition to Application<br />

Number<br />

Filing Date<br />

:NA<br />

:NA<br />

2)SUNDAR, LATHA<br />

(62) Divisional to Application Number :NA<br />

Filing Date<br />

:NA<br />

(57) Abstract :<br />

This invention provides a method for simultaneously detecting the presence <strong>of</strong> at least two enzymes in a sample, said method<br />

comprising the Steps <strong>of</strong>; i) providing a first substrate for a first enzyme, said first substrate being labeled with a first fluorophore, ii)<br />

providing a second substrate for a second enzyme, said second substrate being labeled with a second fluorophore, iii) exposing the<br />

labeled substrates to the sample to allow the first <strong>and</strong> second enzymes present in the sample to interact with respective first <strong>and</strong> second<br />

fluorophore-labeled substrates to form respective first <strong>and</strong> second fluorophore- labeled substrate fragments; <strong>and</strong>, detecting the<br />

presence <strong>of</strong> said fluorophore-labeled substrate fragments.<br />

No. <strong>of</strong> Pages : 34 No. <strong>of</strong> Claims : 32<br />

The Patent Office Journal 24/10/2008 26260

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