continued from part 1 - Controller General of Patents, Designs, and ...

(12) PATENT APPLICATION PUBLICATION (21) Application No.6554/DELNP/2008 A
(19) INDIA
(22) Date of filing of Application :28/07/2008 (43) Publication Date : 24/10/2008
(54) Title of the invention : "ANTIBODY-DEPENDENT CELLULAR CYTOTOXICITY ASSAY"
(51) International classification :G01N 33/50 (71)Name of Applicant :
(31) Priority Document No :60/756,301
1)NOVARTIS AG
(32) Priority Date :04/01/2006
Address of Applicant :LICHTSTRASSE 35, CH-4056 BASEL
(33) Name of priority country :U.S.A.
SWITZERLAND. Switzerland
(86) International Application No :PCT/US2007/060108 (72)Name of Inventor :
Filing Date
:04/01/2007
1)KUNICH JOHN
(87) International Publication No :WO 2007/082138 2)LIU CHENG
(61) Patent of Addition to Application
Number
Filing Date
:NA
:NA
(62) Divisional to Application Number :NA
Filing Date
:NA
(57) Abstract :
Methods for detecting antibody dependent cellular cytotoxicity (ADCC) are described herein. The methods are label-free, and can be
performed in real time on adherent cells. The methods can include, for example, (a) monitoring the impedance between electrodes on
a non-conducting substrate that supports the growth of target cells in an assay medium; and (b) adding effector cells and an antibody
that binds to the target cells to the assay medium; wherein any decrease in the impedance between the electrodes on the substrate
following addition of the effector cells and the antibody is indicative of ADCC function having been effected in the assay medium.
No. of Pages : 55 No. of Claims : 44
The Patent Office Journal 24/10/2008 26022