First International Conference on MOLDAVIAN RISKS – FROM ...

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<strong>First</strong> <strong>International</strong> <strong>Conference</strong> on MOLDAVIAN RISKS - FROM GLOBAL TO LOCAL SCALE 16-19 May 2012, Bacau, Romania DIABETES MELLITUS COMPLICATIONS RISK ASSESSED BY RED BLOOD CELL FTIR PARAMETERS M. Gutu 1 , M. Avadanei 2 , V. Rusu 1 , S. Miron 1 , I. Hurjui 1 , I. Grierosu 1 , C. Stefanescu 1 1 University of Medicine and Pharmacy Iasi “Gr. T. Popa”, Faculty of Medicine, Department of Biophysics and Medical Physics, Iasi 2 “ Petru Poni” Institute of Macromolecular Chemistry, Iasi Corresponding author: Mihai Gutu, misugutu@yahoo.com Abstract: The aim of our study was to determine whether information obtained by FTIR spectral analysis, may be correlated to serum parameters that can be used for diabetes mellitus complications risk evaluation. Material and methods: Two study groups were analyzed. Group 1 consists of 43 subjects clinically healthy, for which analytical blood counts (red blood cell number, leukocyte number, hematocrit (Hct), hemoglobin (Hb), erythrocyte mean volume (MCV), mean erythrocyte hemoglobin (HEM) and some serum biochemical compounds were dosed (serum cholesterol, serum triglycerides, glucose). Group 2 consists of 21 patients diagnosed with complicated type II diabetes. Patients were hospitalized at the Diabetes and nutrition diseases clinic, County Emergency Hospital "St. Spiridon" Iasi in 2011 as emergency. Besides the usual biochemical serum parameters (cholesterol, triglycerides, glucose) we considered serum fibrinogen and glycosilated hemoglobin determination. Erythrocyte samples were analyzed by FTIR spectroscopy by performing erythrocyte films made of 5 µL sample on the support KRS-5 (thallium bromide ioyide), by drying in vacuum (5 mmHg at 20˚C). Correlations between serum parameters, age and FTIR spectral parameters of erythrocyte membrane were assessed. Results show that erythrocyte membrane could be related to the degree of control of diabetes by following spectral parameters and serum parameters specific to diabetes (glycosylated hemoglobin and glucose). There is a relation between ν(=CH)/ν(CHtotal) (global degree of unsaturation lipid) strongly and significantly correlated with blood glucose (r = -0.624, p
<strong>First</strong> <strong>International</strong> <strong>Conference</strong> on MOLDAVIAN RISKS - FROM GLOBAL TO LOCAL SCALE 16-19 May 2012, Bacau, Romania MULTIVARIATE ANALYSIS OF PHYSICO-CHEMICAL DATA CHARACTERISTICS OF WASTES WITH POTENTIAL RADIOACTIVE RISK NEAR BACAU CITY Sorina Zirnea 1 , Iuliana Lazar 2 , Brice Ulrich Saha Foudjo 2, 3 , Gabriel Lazar 1 1 “Vasile Alecsandri” University of Bacau, Department of Mechanical and Environmental Engineering 2 “Vasile Alecsandri” University of Bacau, Department of Chemical and Food Engineering 3 University of Yaoundé I, Cameroon , Dep. of Biochemistry Corresponding author: Sorina Zirnea, sorina.zirnea@ub.ro Abstract: An important environmental issue for Romania is the industrial wastes and their reintroduction in business and in all its sectors. The problem of valorization of these wastes is currently discussed under technical and economic, and in particular ecological aspects. A specific case of hazardous waste is the radioactive waste. Among the main types of hazardous waste present in appreciable quantities in our country are those generated by the chemical industry sector prevailing the waste of soda ash (Alba, Dolj and Valcea County) and phosphogypsum (Bacau County). Phosphogypsum is a waste by-product of phosphoric acid (H3PO4), an essential component used in the fertilizer industry is mostly disposed of without any treatment, usually being disposed in phosphogypsum dumps in close to phosphoric acid plants or being thrown into water or recycled. Per tonne of phosphoric acid produced are generated about five tones of phosphogypsum. In 2007, the study of characterization of phosphogypsum from Platform Sofert S.A. Bacau, made by I.C.I.M. Bucharest, shows that in terms of chemical composition, the waste is not hazardous because it contains heavy metals (0.1%) and mineral compounds of phosphorus (0.367 to 0.473%). Thus, in 2007 following physico-chemical parameters of phosphogypsum samples: humidity, loss on calcinations and silica, phosphorus content, alkali metals content, alkaline earth and heavy metals after specific mineralization for each metal were analyzed in accordance to applicable standards. Until now, such analysis was not repeated. Further investigation was conducted by Regional Agency of Environmental Protection Bacau (RAEP) and was limited to measuring β radioactivity of phosphogypsum and soil samples using the CMR beta measuring system with ND-304 scintillation probe. In this study our aim is to analyze public data from this area in order to be able to make a descriptive statistics interpretation and perform a cluster analysis to identify potential risks and correlations. Evaluation of potential characteristics of taken samples was performed using analytical hierarchy process (AHP) and cluster analysis. The results obtained revealed a marked reduction of radioactivity in the soil in 2011 but also an increase over the average radioactivity in the phosphogypsum during the years 2007-2010. From this preliminary analysis we notice the need to resume a complex analysis at the environment level in the area of the potential risk of radioactive contamination. Keywords: phosphogypsum, β radioactivity, descriptive statistics, cluster analysis. 99
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