DESCRIPTIONS OF MEDICAL FUNGI
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Descriptions of Medical Fungi 49<br />
Candida rugosa complex<br />
Candida rugosa has recently been recognised as a species complex of C. rugosa, C.<br />
pseudorugosa and another as yet undescribed species (Li et al. 2006, Paredes et al.<br />
2012). These species are best identified by ITS sequencing.<br />
RG-1 organism.<br />
Candida rugosa (Anderson) Diddens & Lodder<br />
Culture: Colonies (SDA) white to cream-coloured smooth, glabrous, yeast-like.<br />
Microscopy: Ellipsoidal to elongate budding blastoconidia, 5-11 x 1.5-2.5 µm.<br />
Sometimes short pseudohyphae may be produced.<br />
India Ink Preparation: Negative - no capsules present.<br />
Dalmau Plate Culture: Densely branched pseudohyphae produced.<br />
Physiological Tests: + Positive, - Negative, v Variable, w Weak, s Slow<br />
Germ Tube - L-Sorbose v L-Arabinose - D-Glucitol v<br />
Fermentation Sucrose - D-Arabinose - α-M-D-glucoside -<br />
Glucose - Maltose - D-Ribose - D-Gluconate v<br />
Galactose - Cellobiose - L-Rhamnose - DL-Lactate v<br />
Sucrose - Trehalose - D-Glucosamine - myo-Inositol -<br />
Maltose - Lactose - N-A-D-glucosamine + 2-K-D-gluconate -<br />
Lactose - Melibiose - Glycerol + D-Glucuronate -<br />
Trehalose - Raffinose - Erythritol - Nitrate -<br />
Assimilation Melezitose - Ribitol - Urease -<br />
Glucose + Soluble Starch - Galactitol - 0.1% Cycloheximide -<br />
Galactose + D-Xylose + D-Mannitol v Growth at 37 O C +<br />
Key Features: Germ tube negative yeast and sugar assimilation pattern (D-Xylose<br />
and Glycerol +ve; Ribitol -ve.). C. rugosa has been associated with catheter related<br />
fungaemia and has been isolated from human and bovine faeces, sea water and soil.<br />
Antifungal Susceptibility: C. rugosa limited data (Diekema et al. 2009, Espinel-<br />
Ingroff et al. 2014 and Australian National data); MIC µg/mL.<br />
No 64<br />
AmB 21 1 1 5 5 7 1 1<br />
FLU 97 1 3 15 24 29 8 8 6 1 2<br />
VORI 80 4 8 38 16 4 7 3<br />
POSA 66 1 4 12 27 9 8 5<br />
ITRA 5 1 1 1 1 1<br />
ANID 20 3 4 4 4 3 2<br />
MICA 20 2 3 5 6 2 2<br />
CAS 25 1 1 2 3 10 1 1 2