DESCRIPTIONS OF MEDICAL FUNGI

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Descriptions of Medical Fungi
Candida parapsilosis complex
Candida orthopsilosis Tavanti et al.
RG-1 organism.
Culture: Colonies (SDA) white to cream-coloured smooth, glabrous, yeast-like.
Microscopy: Ellipsoid to subglobose budding blastoconidia, 2-5 x 3-7 µm, with some
larger elongated forms present.
India Ink Preparation: Negative - no capsules present.
Dalmau Plate Culture: Abundant, much-branched pseudohyphae produced.
Physiological Tests: + Positive, - Negative, v Variable, w Weak, s Slow
Germ Tube - L-Sorbose + L-Arabinose + D-Glucitol +
Fermentation Sucrose + D-Arabinose - α-M-D-glucoside +
Glucose + Maltose + D-Ribose - D-Gluconate +
Galactose - Cellobiose - L-Rhamnose - DL-Lactate -
Sucrose - Trehalose + D-Glucosamine - myo-Inositol -
Maltose - Lactose - N-A-D-glucosamine + 2-K-D-gluconate +
Lactose - Melibiose - Glycerol + D-Glucuronate -
Trehalose - Raffinose - Erythritol - Nitrate -
Assimilation Melezitose + Ribitol + Urease -
Glucose + Soluble Starch v Galactitol - 0.1% Cycloheximide -
Galactose + D-Xylose + D-Mannitol + Growth at 37 O C +
Key Features: Candida orthopsilosis cannot be distinguished morphologically from C.
parapsilosis and C. metapsilosis, but can be identified by ITS sequencing (Asadzadeh
et al. 2009, Borman et al. 2009, Tavanti et al. 2005) and MALDI-TOF MS analysis.
Antifungal Susceptibility: C. orthopsilosis (Diekema et al. 2009, Canton et al.
2012, 2013 and Australian National data); MIC µg/mL.
No 64
AmB 224 3 3 30 45 60 48 23 9
FLU 196 1 9 45 85 24 16 9 4 3
VORI 176 28 40 51 35 6 13 2 2
POSA 136 5 21 49 41 9 11
ITRA 94 1 2 14 30 32 15
ANID 86 1 2 9 20 44 10
MICA 85 1 1 3 36 37 6 1
CAS 146 2 4 18 45 36 27 10 4
5FC 92 56 22 8 3 1 1 1