DESCRIPTIONS OF MEDICAL FUNGI
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Descriptions of Medical Fungi 45<br />
RG-1 organism.<br />
Candida parapsilosis complex<br />
Recently Candida parapsilosis has been recognised as a complex of four species:<br />
C. parapsilosis, C. orthopsilosis, C. metapsilosis and Lodderomyces elongisporus<br />
(Tavanti et al. 2005). These four species are phenotypically indistinguishable and are<br />
best identified by ITS sequencing or MALDI-T<strong>OF</strong> MS analysis.<br />
Candida metapsilosis Tavanti et al.<br />
Culture: Colonies (SDA) white to cream-coloured smooth, glabrous, yeast-like.<br />
Microscopy: Ellipsoid, subglobose to fusiform budding blastoconidia, 4 x 3-6 µm, with<br />
some larger subglobose forms present.<br />
India Ink Preparation: Negative - no capsules present.<br />
Dalmau Plate Culture: Abundant, much branched pseudohyphae produced.<br />
Physiological Tests: + Positive, - Negative, v Variable, w Weak, s Slow<br />
Germ Tube - L-Sorbose + L-Arabinose + D-Glucitol +<br />
Fermentation Sucrose + D-Arabinose - M-D-glucoside +<br />
Glucose + Maltose + D-Ribose + D-Gluconate +<br />
Galactose - Cellobiose - L-Rhamnose - DL-Lactate -<br />
Sucrose - Trehalose + D-Glucosamine - myo-Inositol -<br />
Maltose - Lactose - N-A-D-glucosamine + 2-K-D-gluconate +<br />
Lactose - Melibiose - Glycerol + D-Glucuronate -<br />
Trehalose - Raffinose - Erythritol - Nitrate -<br />
Assimilation Melezitose + Ribitol + Urease -<br />
Glucose + Soluble Starch v Galactitol - 0.1% Cycloheximide -<br />
Galactose + D-Xylose + D-Mannitol + Growth at 37 O C +<br />
Key Features: Candida metapsilosis cannot be distinguished morphologically from C.<br />
parapsilosis and C. orthopsilosis, but can be identified by ITS sequencing (Asadzadeh<br />
et al. 2009, Borman et al. 2009, Tavanti et al. 2005) and MALDI-T<strong>OF</strong> MS analysis.<br />
Antifungal Susceptibility: C. metapsilosis limited data (Diekema et al. 2009 and<br />
Australian National data); MIC µg/mL.<br />
No 64<br />
AmB 32 1 5 12 11 2 1<br />
FLU 32 1 19 10 2<br />
VORI 32 1 4 22 3 2<br />
POSA 32 1 7 16 6 1<br />
ITRA 2 1 1<br />
ANID 13 2 5 3 2 1<br />
MICA 13 9 3 1<br />
CAS 26 1 5 15 4 1<br />
5FC 2 2<br />
Note: Additional data for ITRA MIC range 0.06-0.5, MIC 90<br />
= 0.25; and 5FC MIC range 0.06-<br />
64, MIC 90<br />
= 0.5 (Gomez-Lopez et al. 2008, Miranda-Zapico et al. 2011).
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