DESCRIPTIONS OF MEDICAL FUNGI

Descriptions of Medical Fungi 39
Candida dubliniensis Sullivan et al.
Candida dubliniensis is an occasional cause of candidaemia and mucosal infection,
especially in HIV patients.
RG-2 organism.
Culture: Colonies (SDA) white to cream-coloured smooth, glabrous, yeast-like.
Microscopy: Spherical to subspherical budding blastoconidia, 3-8 x 2-7 µm in size.
India Ink Preparation: Negative - no capsules present.
Dalmau Plate Culture: Branched pseudohyphae with dense verticils of blastoconidia
and spherical, mostly terminal chlamydospores.
Physiological Tests: + Positive, - Negative, v Variable, w Weak, s Slow
Germ Tube + L-Sorbose - L-Arabinose - D-Glucitol +
Fermentation Sucrose + D-Arabinose - M-D-glucoside +,s
Glucose + Maltose + D-Ribose - D-Gluconate -
Galactose +,s Cellobiose - L-Rhamnose - DL-Lactate +
Sucrose - Trehalose s,+ D-Glucosamine v myo-Inositol -
Maltose + Lactose - N-A-D-glucosamine + 2-K-D-gluconate +
Lactose - Melibiose - Glycerol w,s,+ D-Glucuronate -
Trehalose v Raffinose - Erythritol - Nitrate -
Assimilation Melezitose w,+ Ribitol + Urease -
Glucose + Soluble Starch w,+ Galactitol - 0.1% Cycloheximide +
Galactose + D-Xylose s,+ D-Mannitol + Growth at 40 O C +
Key Features: Germ tube positive, similar to C. albicans, except for absence of growth
at 42 O C; glycerol (mostly +), methyl-a-D-glucoside (-), trehalose (-), and D-xylose (-).
Initial colonies dark green on CHROMagar and producing rough colonies on bird seed
agar. ITS sequencing and MALDI-TOF can reliably distinguish C. dubliniensis from C.
albicans.
Antifungal Susceptibility: C. dubliniensis (Australian National data); MIC µg/mL.
No 64
AmB 74 1 1 3 28 30 10 1
FLU 74 24 24 21 5
VORI 71 67 4
POSA 63 15 19 18 8 3
ITRA 74 6 22 14 22 6 4
ANID 49 1 7 12 9 12 2 6
MICA 49 4 11 18 8 2 6
CAS 66 8 23 17 9 1 1 7
5FC 74 12 49 7 4 1 1