DESCRIPTIONS OF MEDICAL FUNGI
fungus3-book
fungus3-book
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
Descriptions of Medical Fungi 27<br />
Aureobasidium pullulans has a worldwide distribution and is usually isolated as<br />
a saprophyte, occasionally from skin and nails. It has also been reported as a rare<br />
causative agent of phaeohyphomycosis, mycotic keratitis and peritonitis in patients on<br />
continuous ambulatory peritoneal dialysis (CAPD).<br />
RG-1 organism.<br />
Aureobasidium pullulans (de Bary) Arnaud<br />
Morphological Description: Colonies are fast growing, smooth, soon covered with<br />
slimy masses of conidia, cream or pink, later becoming brown or black. Hyphae are<br />
hyaline and septate, frequently becoming dark-brown with age and forming chains of<br />
one to two-celled, thick-walled, darkly pigmented arthroconidia. These arthroconidia<br />
actually represent the Scytalidium anamorph of Aureobasidium and are only of<br />
secondary importance in recognising members of this genus. Conidia are produced<br />
synchronously in dense groups from indistinct scars or from short denticles on<br />
undifferentiated, hyaline to subhyaline hyphae. Conidia are hyaline, smooth-walled,<br />
single-celled, ellipsoidal but of variable shape and size (8-12 x 4-6 µm), often with an<br />
indistinct hilum (i.e. a mark or scar at the point of attachment). Temperature: optimum<br />
25 O C; maximum 35-37 O C.<br />
Molecular Identification: Recommended barcoding genes are ITS, EF-1α and D1/D2<br />
(de Hoog et al. 2015).<br />
Key Features: Hyphomycete (so-called black yeast) producing hyaline blastoconidia<br />
simultaneously from the vegetative hyphae, which may also form chains of darkly<br />
pigmented, thick-walled arthroconidia.<br />
References: Hermanides-Nijhof (1977), Domsch et al. (2007), McGinnis (1980), de<br />
Hoog et al. (2000, 2015), Najafzadeh et al. (2014).<br />
20 μm<br />
Aureobasidium pullulans showing one to two-celled, darkly pigmented arthroconidia<br />
and hyaline, single-celled, ovoid-shaped conidia which are produced on short denticles.<br />
Antifungal Susceptibility: A. pullulans data from 108 isolates (Najafzadeh et al.<br />
2014 and Australian National data); MIC µg/mL.<br />
Antifungal Range MIC 90<br />
Antifungal Range MIC 90<br />
AmB 0.008-16 1 VORI 0.008-16 2<br />
ITRA 0.008-16 0.5 POSA 0.008-4 0.5