DESCRIPTIONS OF MEDICAL FUNGI

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Descriptions of Medical Fungi
Tap Water Agar
For the stimulation of sporulation in Apophysomyces and Saksenaea isolates.
Bacto agar (BD 214010)
15 g
Distilled water
1000 mL
1. Add agar to water in stainless steel jug, allow to soak.
2. Dispense for slopes.
3. Autoclave at 121 O C for 10 minutes, remove and slope.
Urea Agar Slopes with 0.5% Glucose.
For the differentiation of urease producing organisms.
Urea glucose broth base:
Urea, broth base (Oxoid CM71)
0.9 g
Glucose
5 g
Distilled water
450 mL
1. Add the Urea broth base and glucose to the distilled water in a 500mL
beaker.
2. Dispense in 5 x 90 mL amounts.
3. Autoclave at 121 O C for 20 mins.
4. When cool, label and store in the fridge.
Method to make slopes:
SPECIALISED CULTURE MEDIA
40% Urea Solution (Oxoid SR 20) 10 mL
Bacto agar (BD 214010)
3 g
Distilled water
100 mL
1. Add agar to 100 mL of distilled water in a 250 mL pyrex bottle.
2. Autoclave at 121 O C for 15 minutes and place in 50 O C water bath.
3. When cool add 90 mL of the Urea broth with glucose and the 10 mL
of 40% urea solution to agar and dispense in 3 mL aliquots and angle
bottles to form slopes on racks.
Vitamin Free Agar (Trichophyton Agar No.1)
For the differentiation of Trichophyton species.
Trichophyton agar No. 1 (BD 287710)
11.8 g
Distilled water
200 mL
1. Add agar to water in stainless steel jug, allow to soak.
2. Bring to boil to dissolve, stirring constantly.
3. Once boiled remove immediately to avoid discolouration.
4. Dispense for slopes.
5. Autoclave at 121 O C for 10 minutes, remove and slope.