DESCRIPTIONS OF MEDICAL FUNGI

Descriptions of Medical Fungi 239
SPECIALISED CULTURE MEDIA
Sabouraud’s Dextrose Agar (SDA) with Cycloheximide (0.05%),
Chloramphenicol and Gentamicin
For the primary isolation and cultivation of dermatophytes.
Sabouraud’s dextrose agar (Oxoid CM41)
65 g
Cycloheximide (Actidione)
0.5 g
Chloramphenicol
0.05 g
Gentamicin (40mg/mL)
0.56 mL
Yeast extract
5 g
Distilled water
1000 mL
1. Soak all ingredients, except gentamicin, in 100 mL water.
2. Boil remaining water, add to soaking ingredients, and bring to boil to
dissolve, stirring well to prevent from burning.
3. Add the gentamicin. Mix well.
4. Dispense for slopes as required.
5. Autoclave at 121 O C for 10 minutes. Remove and slope, or pour for plates
as required.
Sabouraud’s Dextrose Agar with Chloramphenicol and Gentamicin.
For primary isolation and routine culture of yeasts and moulds.
Sabouraud’s dextrose agar (Oxoid CM41)
65 g
Chloramphenicol
0.05 g
Gentamicin (40mg/mL)
0.56 mL
Distilled water
1000 mL
See above method for Sabouraud dextrose agar with cycloheximide,
chloramphenicol and gentamicin.
Sabouraud’s Dextrose Agar with 5% Salt.
For the differentiation of Trichophyton species.
Sabouraud dextrose agar (Oxoid CM41)
Sodium Chloride NaCl (Univar 465)
Distilled water
1. Soak dry ingredients in approximately 100 mL water.
2. Bring remaining water to boil, add to soaking ingredients.
3. Dispense into McCartney bottles for slopes (7 mL).
4. Autoclave at 121 O C for 10 minutes, then slope on racks.
32.5 g
25 g
500 mL