DESCRIPTIONS OF MEDICAL FUNGI

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Descriptions of Medical Fungi
SPECIALISED CULTURE MEDIA
Malt Extract Agar
For routine cultivation and identification of fungi.
Oxoid Malt Extract (L39)
20 g
Bacto agar (BD 214010)
20 g
Distilled water
1000 mL
1. Dissolve malt extract in a beaker and adjust the solution to pH 6.5 with
NaOH.
2. Soak agar in small quantity of solution. Bring malt extract solution to
the boil, stirring constantly.
3. Add to soaking agar. Bring to boil, stirring constantly.
4. Dispense for slopes as required.
5. Autoclave at 121 O C for 10 minutes, remove and then angle to form
slopes on racks or pour for plates as required.
1% Peptone Agar
For the differentiation of Trichophyton species.
Tryptone Peptone (BD 211705)
5 g
Bacto agar (BD 214010)
10 g
Distilled water
500 mL
1. Soak agar and peptone in about 50 mL of water.
2. Boil remaining water, add to the soaking ingredients and bring back to
the boil again.
3. Dispense for slopes (7 mL).
4. Autoclave for 10 minutes at 121 O C, then angle to form slopes.
Potato Dextrose Agar.
For routine cultivation and identification of fungi.
Potato dextrose agar (Oxoid CM139)
39 g
Distilled water
1000 mL
1. Soak potato dextrose agar in 100 mL of the water.
2. Boil remaining water, add to soaking potato dextrose agar, bring back
to the boil, stirring constantly.
3. Dispense for slopes as required.
4. Autoclave at 121 O C for 15 minutes. Remove and slope or pour for
plates as required.
Rice Grain Slopes.
To induce sporulation and for differentiation of M. audouinii and M. canis.
Polished rice grains
Distilled water
1. Place ~ 0.5 teaspoon rice grains into wide neck 20 mL glass vials.
2. Add 8 mL distilled water to each vial.
3. Loosely cap the vials, then angle to form slopes on racks ensuring rice
grains are evenly distributed.
4. Autoclave racks at 121 O C for 15 minutes.