DESCRIPTIONS OF MEDICAL FUNGI

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Descriptions of Medical Fungi
SPECIALISED CULTURE MEDIA
Cornmeal Agar
For routine cultivation and identification of fungi.
Cornmeal agar (Oxoid CM0103)
8.5 g
Distilled water
500 mL
1. Mix dry ingredients into 100 mL water, boil remaining water.
2. Add boiling water to mixture and bring to boil.
3. Autoclave for 10 minutes at 121 O C, then slope on racks.
Cornmeal Glucose Sucrose Yeast Extract Agar
For mucormycete sporulation.
Cornmeal agar (Oxoid CM0103)
17 g
Dextrose (Glucose)
2 g
Sucrose
3 g
Yeast extract
1 g
Distilled water
1000 mL
1. Soak dry ingredients in 100 mL water, then boil remaining water.
2. Add boiling water to mixture and bring to boil.
3. Dispense for slopes.
4. Autoclave for 10 minutes at 121 O C, remove and then angle to form
slopes on racks.
Czapek Dox Agar
For routine cultivation of fungi, especially Aspergillus, Penicillium, and non-sporulating
moulds.
Czapek Dox agar (Oxoid CM97)
45.4 g
Distilled water
1000 mL
1. Soak the ingredients in small amount of water.
2. Bring remaining water to boil, add to soaking ingredients and bring to
the boil again, stirring continuously.
3. Dispense for slopes as required.
4. Autoclave at 121 O C for 10 minutes, remove and slope or pour for
plates as required.
Modified Dixon’s Agar
For primary isolation and cultivation of Malassezia species.
Malt extract (Oxoid L39) 9 g Bacto Tryptone 1.5 g
Ox-bile Desiccated (Oxoid L50) 5 g Tween 40 2.5 mL
Oleic acid 0.5 g Glycerol 0.5 mL
Bacto agar 3 g Distilled water 250 mL
1. Soak ingredients in a little of the water.
2. Bring remaining water to boil, add to the soaking ingredients and bring
to the boil again constantly stirring.
3. Dispense for slopes (7 mL) into 30 mL bottles.
4. Autoclave at 121 O C for 10 minutes and then angle to form slopes on
racks.