DESCRIPTIONS OF MEDICAL FUNGI
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190<br />
Descriptions of Medical Fungi<br />
Talaromyces marneffei (Segretain et al.) Samson et al.<br />
Synonymy: Penicillium marneffei Segretain et al.<br />
WARNING: RG-3 organism. Cultures of Talaromyces marneffei may represent a<br />
biohazard to laboratory personnel and should be handled with caution in a class II<br />
Biological Safety Cabinet (BSCII). T. marneffei exhibits thermal dimorphism and is<br />
endemic in Southeast Asia and the southern region of China.<br />
Samson et al. (2011b) redefined Talaromyces by combining Penicillium subgenus<br />
Biverticillium into Talaromyces based upon phylogenetic analysis of the ITS and RPB1<br />
loci. The genus contains 88 species that were placed into seven sections based on a<br />
multigene phylogeny of the ITS, β-tubulin and RPB2 regions (Yilmaz et al. 2014). T.<br />
marneffei is the only known dimorphic species in the genus, producing filamentous<br />
growth at 25 O C and a yeast phase at 37 O C (Andrianopoulos 2002).<br />
Molecular Identification: ITS sequencing is recommended, as well as β-tubulin as a<br />
secondary molecular marker for identification (Yilmaz et al. 2014).<br />
Morphological Description: Colonies at 25 O C are fast growing, suede-like to downy,<br />
white with yellowish-green conidial heads. Colonies become greyish-pink to brown<br />
with age and produce a diffusible brownish-red to wine-red pigment. Conidiophores<br />
generally biverticillate and sometimes monoverticillate; hyaline, smooth-walled and<br />
bear terminal verticils of three to five metulae, each bearing three to seven phialides.<br />
Phialides are acerose to flask-shaped. Conidia are globose to subglobose, 2-3 µm in<br />
diameter, smooth-walled and are produced in basipetal succession from the phialides.<br />
On brain heart infusion (BHI) agar containing blood incubated at 37 O C, colonies are<br />
rough, glabrous, tan-coloured and yeast-like. Microscopically, yeast cells are spherical<br />
to ellipsoidal, 2-6 µm in diameter, and divide by fission rather than budding. Numerous<br />
short hyphal elements are also present.<br />
Histopathology: Tissue sections show small, oval to ellipsoidal yeast-like cells, 3 µm in<br />
diameter, either packed within histiocytes or scattered through the tissue. Occasional,<br />
large, elongated sausage-shaped cells, up to 8 µm long, with distinctive septa may be<br />
present.<br />
Key Features: Talaromyces marneffei is the only dimorphic species of Talaromyces,<br />
which grows as a yeast at 37 O C. It produces a red soluble pigment on general media<br />
and conidiophores have flask-shaped to acerose phialides.<br />
References: Pitt (1979), Ramirez (1982), de Hoog et al. (2000, 2015), Andrianopoulos<br />
(2002), Lyratzopoulos et al. (2002), Samson et al. (2011b), Visagie et al. (2014), Yilmaz<br />
et al. (2014).