DESCRIPTIONS OF MEDICAL FUNGI

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142 Descriptions of Medical Fungi Neoscytalidium dimidiatum (Penzig) Crous & Slippers 20 µm Neoscytalidium dimidiatum showing chains of one to two celled, darkly pigmented arthroconidia. Antifungal Susceptibility: Neoscytalidium dimidiatum limited data (Australian National data); MIC µg/mL. No. 64 AmB 3 1 1 1 VORI 3 1 1 1 POSA 3 1 1 1 ITRA 3 1 1 1 N. dimidiatum data from 24 isolates (Madrid et al. 2009); MIC µg/mL. AmB Range 0.125-0.5; Geometric mean = 0.32 VORI Range 0.06-4; Geometric mean = 1.37 POSA Range 0.06-32; Geometric mean = 6.15 N. dimidiatum data from 10 isolates (Espinel-Ingroff et al. 2001); MIC µg/mL. ITRA Range 0.03-32; Geometric mean = 0.65
Descriptions of Medical Fungi 143 Ochroconis de Hoog and Arx Recently, the genus Ochroconis has undergone taxonomic revision, and the most relevant species, Ochroconis gallopava, was transferred to the new genus Verruconis (Samerpitak et al. 2014). Ochroconis species are mesophilic saprobes, with an optimum growth temperature between 15 and 30 O C and an inability to grow at 37 O C. Nevertheless, some species have been isolated from clinical specimens; such as Ochroconis tshawytschae, O. mirabilis, O. cordanae, O. olivacea and O. ramosa (Samerpitak et al. 2014, Seyedmousavi et al. 2014, Giraldo et al. 2014). Note: Species described in the literature from clinical cases as O. constricta or O. humicola are probably O. musae (Samerpitak et al. 2014). Giraldo et al. (2014) reported on the occurrence of Ochroconis and Verruconis species in clinical specimens from the United States. V. gallopava was the most common species (69%), followed by O. mirabilis (22%). Other species isolated were O. cordanae, O. olivacea and O. ramosa. The most common anatomical site of isolation was the lower respiratory tract (59%), followed by superficial (22%) and deep tissues (20%) (Giraldo et al. 2014). RG-1 organisms. Morphological Description: Colonies restricted, velvety to funiculose, brown to olivaceous, often with rust-brown reverse. Hyphae smooth- or somewhat rough-walled, pale olivaceous. Conidiophores slightly or conspicuously differentiated, cylindrical, often flexuose, producing conidia on scattered, cylindrical to conical denticles. After detachment an inconspicuous frill often remains both on the denticle and on the conidium base. Conidia one to four-celled, pale olivaceous brown, smooth- or roughwalled, ellipsoidal, cylindrical, clavate or cuneiform. Maximum growth temperature around 33 O C. Molecular Identification: Giraldo et al. (2014) used sequence analyses of the 18S, ITS, D1/2, actin, and β-tubulin genes, while Seyedmousavi et al. (2014), used ITS sequence analyses to identify species. References: de Hoog et al. (2015), Samerpitak et al. (2014), Seyedmousavi et al. (2014), Giraldo et al. (2014). Antifungal Susceptibility: Ochroconis mirabilis variable data for posaconazole, caspofungin and anidulafungin from Seyedmousavi et al. (2014) 1 and Giraldo et al (2014) 2 ; MIC µg/mL. Antifungal Range MIC 90 Antifungal Range MIC 90 0.06-0.25 AmB 1-32 32 POSA 1 - 0.5-32 2 32 ITRA 0.25-32 32 VORI 2-32 32 CAS 0.5-1 1 1-32 2 - 4 ANID 0.03-0.125 1 0.015-32 2 - 4 MICA 0.06-0.50 0.25 TERB 0.015-0.125 0.02
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DESCRIPTIONS OF MEDICAL FUNGI

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