DESCRIPTIONS OF MEDICAL FUNGI

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Descriptions of Medical Fungi
Meyerozyma guilliermondii (Wick.) Kurtzman & M. Suzuki
Synonymy: Candida guilliermondii (Castellani) Langeron & Guerra.
Meyerozyma guilliermondii has been isolated from numerous human infections, mostly
of cutaneous origin. It is also found on normal skin and in sea water, faeces of animals,
fig wasps, buttermilk, leather, fish and beer. RG-1 organism.
Culture: White to cream-coloured smooth, glabrous, yeast-like colonies.
Microscopy: Spherical to subspherical budding yeast-like cells or blastoconidia, 2.0-
4.0 x 3.0-6.5 µm.
India Ink Preparation: Negative - no capsules present.
Dalmau Plate Culture: Branched pseudohyphae with dense verticils of blastoconidia.
Molecular Identification: ITS sequencing recommended.
MALDI-TOF MS: Able to accurately identify this species.
Physiological Tests: + Positive, - Negative, v Variable, w Weak, s Slow, nd No Data
Germ Tube - L-Sorbose v L-Arabinose v D-Glucitol v
Fermentation Sucrose + D-Arabinose v α-M-D-glucoside v
Glucose + Maltose + D-Ribose + D-Gluconate v
Galactose v Cellobiose v L-Rhamnose v DL-Lactate v
Sucrose + Trehalose + D-Glucosamine + myo-Inositol -
Maltose - Lactose - N-A-D-glucosamine + 2-K-D-gluconate +
Lactose - Melibiose v Glycerol + D-Glucuronate nd
Trehalose + Raffinose + Erythritol - Nitrate -
Assimilation Melezitose v Ribitol + Urease -
Glucose + Soluble Starch - Galactitol v 0.1% Cycloheximide v
Galactose + D-Xylose + D-Mannitol v Growth at 37 O C v
Key Features: Germ tube negative yeast and sugar assimilation pattern.
Antifungal Susceptibility: M. guilliermondii (Diekema et al. 2009, Australian
National data); MIC µg/mL. CLSI clinical breakpoints are marked where available
(Pfaller and Diekema 2012).
No. 64
AmB 198 1 1 2 9 70 70 27 9 2 1 1 5
FLU 199 1 4 5 75 75 7 11 4 7
VORI 197 2 11 23 88 52 9 3 1 2 6
POSA 194 1 9 14 47 79 30 4 4 6
ITRA 24 6 3 12 2 1
ANID 120 1 2 6 5 9 41 48 8
MICA 13 3 1 4 10 14 39 29 8 1
CAS 175 2 9 24 35 63 25 8 2 3 4
5FC 24 8 12 1 1 1 1