Database on medicinal plants used in ayurveda - AYUSH Research ...

0.5 mg/L Kn showed multiple shoot formation. These calli when placed on
hormone free medium showed enhanced shoot differentiation, Dieu and
Dunwell, (1988).
Regeneration and in vitro flowering in P. somniferum was also reported by
Yoshikawa and Furaya, (1983). Secondary metabolites i.e. codeine was
obtained through tissue culture (Furuya et al., 1984).
Tyler et al., (1988) reported effect of ethylene on sanguinarine production in
cell cultures of P. somniferum. Cell cultures were established from hypocotyl
explants cultured in B5 medium containing 1 mg/L 2, 4-D and 1g/l casein
hydrolysate. Callus raised was used in suspension cultures (approx 4g callus
inoculated into 75 ml 1B5C) and subcultured weekly. The suspensions were
elicited by adding 0.2 ml Botrytis homogenate to 7-day old cultures. Cells
for sanguinarine were harvested after 2 days of cultures.
Similarly, Songstad and Coworkers (1989), reported the effect of ethylene
and ACC on production of sanguinarine in liquid or solid culture medium in
presence or absence of elicitors. They also observed that cells utilized ACC
(1-aminocyclopropane carboxylic acid) in their early stage of development.
REFERENCES
Aceto MD; Harris LS; Abood ME; Rice KC (1999), Stereoselective �- and δ-opioid
receptor-related antinociception and binding with (+)-thebaine. Eur J Pharmacol. 365(2-3) :
143-147.
Alam MM; Ashraf SM; Hakim MH (1994), Antihypertensive effect of Saffof Khashkhash
and Sarbat Bazoori Moatadil in essential hypertension- a clinical study. Hamdard Medicus.
37(3) : 130-133.
Alcantara J; Bird DA; Franceschi VR; Facchini PJ (2005), Sanguinarine biosynthesis
associated with the endoplasmic reticulum in cultured opium poppy cells after elicitor
treatment. Plant Physiol. 138(1) : 173-83.
Alonso Osorio MJ (2004), [Useful medicinal plants]. Rev Enferm. 27(3) : 8-12.
Annette T; Bose G (1921-23), Chemical constituents of Papaver somniferum. Dep Agri
India, Chem. 6: 215.
Anonymous (1966), The Wealth of India, Raw Materials, Publications and Information
Directorate, Council of Scientific and Industrial Research, New Delhi. vol. VII. p. 233-248.
Anonymous (1978), The Ayurvedic Formulary of India, Ministry of Health and Family
welfare, Dept of ISM & H, Govt. of India, New Delhi, Part- I.
Anonymous (1995), Indian Medicinal Plants, Ed. Warrier, P.K. et al., Orient Longman Ltd.,
Madras. vol. 2. p. 214-220.
11