Mahidol University Abstract of International Publications 2010
Mahidol University Abstract of International Publications 2010
Mahidol University Abstract of International Publications 2010
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.1<br />
Author (s) : Abu, S.A., Maude, R.J., Hasan, M.U., Hoque, Md.G., Dondorp, A.M., Abul, F.Md.<br />
Title : MALARIAL RETINOPATHY IN BANGLADESHI ADULTS<br />
Source : AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, Volume 83, Issue 5, Supplement S, NOV <strong>2010</strong> Pages 17-17<br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.2<br />
Author (s) : Achariyapota, V., Benjapibal, M., Chaopotong, P.<br />
Title : Prevalence and incidence <strong>of</strong> anemia in thai patients with gynecologic cancer<br />
Source : Asian Pacific Journal <strong>of</strong> Cancer Prevention, Volume 11, Issue 5, <strong>2010</strong>, Pages 1229-1233<br />
Document Type : Article<br />
Keywords : Anemia; Cancer; Frequency; Incidence; Prevalence<br />
<strong>Abstract</strong> : This prospective, single institute, 6-month observational survey aimed to evaluate the prevalence, incidence, frequency,<br />
treatment <strong>of</strong> anemia, and trigger hemoglobin (Hb) level for initiating transfusion in patients with gynecologic malignancy. One<br />
hundred and eighty-six consecutive patients with gynecologic malignancy were analyzed between June and December 2009.<br />
Hb level data were collected for up to six data points or 6 months <strong>of</strong> scheduled visits. Tumor type, disease status, cancer<br />
treatment and anemia treatment as well as trigger Hb level for starting treatment were evaluated. The mean age <strong>of</strong> patients<br />
was 51 years. Prevalence <strong>of</strong> anemia at enrollment was 66.1% (123/186), with 36 <strong>of</strong> 186 patients (19.4%) having moderate to<br />
severe anemia (Hb < 10.0 g/dl). The highest prevalence was found among patients with endometrial cancer (72.2%) and<br />
ovarian cancer (72%), newlydiagnosed/ receiving treatment (70.9%) and those receiving radiotherapy (75%). The incidence <strong>of</strong><br />
anemia was 85.7% (54/63). Ovarian cancer had the highest association (87%). For disease status and cancer treatment, the<br />
incidence was highest in patients with persistent/recurrent disease (95.2%) and those who received radiotherapy (100%). One<br />
hundred and seventy-seven <strong>of</strong> 186 patients (95.2%) were ever anemic during the survey. Anemia was frequently reported in<br />
patients with all tumor types (93-100%), persistent/recurrent disease (98.3%) and those who received radiotherapy (100%)<br />
and 80.8% <strong>of</strong> patients who were ever anemic recieved treatment (oral iron, 42.9%; transfusion, 37.3%; and erythropoietic<br />
agent, 0.6%). In conclusion, the mean Hb trigger level for initiating transfusion as treatment <strong>of</strong> anemia was 8.6g/dL. The<br />
prevalence, incidence, and frequency <strong>of</strong> anemia are very high among patients with gynecologic malignancy; especially those<br />
with ovarian cancer, persistent/recurrent disease, and those receiving treatment.<br />
No.3<br />
Author (s) : Adhikari, R.<br />
Title : Are Nepali students at risk <strong>of</strong> HIV? A cross-sectional study <strong>of</strong> condom use at first sexual intercourse among college students in<br />
Kathmandu<br />
Source : JOURNAL OF THE INTERNATIONAL AIDS SOCIETY, Volume 13, Article Number 7, MAR 2 <strong>2010</strong><br />
Document Type : Article<br />
Keywords : TRANSMITTED INFECTIONS; SCHOOL STUDENTS; HARVARD SCHOOL; BINGE-DRINKING; YOUNG-ADULTS; HEALTH; BEHAVIORS;<br />
TANZANIA; ALCOHOL; COMMUNICATION<br />
<strong>Abstract</strong> : Background: Condoms <strong>of</strong>fer the best protection against unintended pregnancies and sexually transmitted infections. Little<br />
research has been conducted to determine the prevalence and investigate the influencing factors <strong>of</strong> condom use at first<br />
sexual intercourse among college students. Methods: A self-administered questionnaire was completed by 1137 college<br />
students (573 male and 564 female) in the Kathmandu Valley. Analyses were confined to 428 students who reported that<br />
they have ever had sexual intercourse. The association between condom use at first sexual intercourse and the explanatory<br />
variables was assessed in bivariate analysis using Chi-square tests. The associations were further explored using multivariate<br />
logistic analysis in order to identify the significant predictors after controlling for other variables. Results: Among the sexually<br />
active students, less than half (48%) had used condoms during first sexual intercourse. The results from the logistic regression<br />
analysis revealed that age, caste and/or ethnicity, age at first sexual intercourse, types <strong>of</strong> first sex partner, alcohol<br />
consumption and mass media exposure are significant predictors for condom use at first sexual intercourse among the<br />
college students. Students in the older age groups who had first sex were about four times (16 to 19 years old) (OR = 3.5)<br />
more likely and nine times (20 or older) (OR = 8.9) more likely than the students who had sex before 16 years <strong>of</strong> age to use<br />
condoms at first sexual intercourse. Moreover, those students who had first sex with commercial sex worker were five times<br />
1
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
(OR = 4.9) more likely than those who had first sex with their spouse to use condoms at first sex. Furthermore, students who<br />
had higher exposure to both print and electronic media were about twice (OR = 1.75) as likely as those who had lower media<br />
exposure to use condoms. On the other hand, students who frequently consumed alcohol were 54% (OR = 0.46) less likely<br />
to use condoms at first sexual intercourse than those who never or rarely consumed alcohol. Conclusions: The rate <strong>of</strong><br />
condom use at first sexual intercourse is low among the students. It indicates students are exposed to health hazards through<br />
their sexual behaviour. If low use <strong>of</strong> condom at first sex continues, vulnerable sexual networks will grow among them that<br />
allow quicker spreading <strong>of</strong> sexually transmitted diseases and HIV. Findings from this study point to areas that policy and<br />
programmes can address to provide youth with access to the kinds <strong>of</strong> information and services they need to achieve healthy<br />
sexual and reproductive lives.<br />
No.4<br />
Author (s) : Adhikari, R.<br />
Title : Demographic, socio-economic, and cultural factors affecting fertility differentials in Nepal<br />
Source : BMC Pregnancy and Childbirth, Volume 10, 28 April <strong>2010</strong>, Article number 19<br />
Document Type : Article<br />
Keywords : age, article, child death, childbirth, cultural factor, demography, educational status, family planning, female<br />
fertility, marriage, mass medium, moslem, Nepal, prediction, religion, residential home, rural area, social<br />
status, socioeconomics, adult, attitude to health, developing country, ethnology, family size, female, fertility, health<br />
survey, human, middle aged, multivariate analysis, parity, pregnancy, social psychology, statistical model; Adult, Attitude to<br />
Health, Demography, Developing Countries, Educational Status, Family Characteristics, Family Planning<br />
Services, Female, Fertility, Health Knowledge, Attitudes, Practice, Health Surveys, Humans, Linear Models, Mass Media, Middle<br />
Aged, Multivariate Analysis, Nepal, Parity, Pregnancy, Residence Characteristics, Social Values, Socioeconomic Factors<br />
<strong>Abstract</strong> : Background: Traditionally Nepalese society favors high fertility. Children are a symbol <strong>of</strong> well-being both socially and<br />
economically. Although fertility has been decreasing in Nepal since 1981, it is still high compared to many other developing<br />
countries. This paper is an attempt to examine the demographic, socio-economic, and cultural factors for fertility differentials<br />
in Nepal.Methods: This paper has used data from the Nepal Demographic and Health Survey (NDHS 2006). The analysis is<br />
confined to ever married women <strong>of</strong> reproductive age (8,644). Both bivariate and multivariate analyses have been performed<br />
to describe the fertility differentials. The bivariate analysis (one-way ANOVA) was applied to examine the association between<br />
children ever born and women's demographic, socio-economic, and cultural characteristics. Besides bivariate analysis, the net<br />
effect <strong>of</strong> each independent variable on the dependent variable after controlling for the effect <strong>of</strong> other predictors has also<br />
been measured through multivariate analysis (multiple linear regressions).Results: The mean numbers <strong>of</strong> children ever born<br />
(CEB) among married Nepali women <strong>of</strong> reproductive age and among women aged 40-49 were three and five children,<br />
respectively. There are considerable differentials in the average number <strong>of</strong> children ever born according to women's<br />
demographic, socio-economic, and cultural settings. Regression analysis revealed that age at first marriage, perceived ideal<br />
number <strong>of</strong> children, place <strong>of</strong> residence, literacy status, religion, mass media exposure, use <strong>of</strong> family planning methods,<br />
household headship, and experience <strong>of</strong> child death were the most important variables that explained the variance in fertility.<br />
Women who considered a higher number <strong>of</strong> children as ideal (β = 0.03; p < 0.001), those who resided in rural areas (β =<br />
0.02; p < 0.05), Muslim women (β = 0.07; p < 0.001), those who had ever used family planning methods (β = 0.08; p <<br />
0.001), and those who had a child-death experience (β = 0.31; p < 0.001) were more likely to have a higher number <strong>of</strong> CEB<br />
compared to their counterparts. On the other hand, those who married at a later age (β = -0.15; p < 0.001), were literate (β<br />
= -0.05; p < 0.001), were exposed to both (radio/TV) mass media (β = -0.05; p < 0.001), were richest (β = -0.12; p < 0.001),<br />
and were from female-headed households (β = -0.02; p < 0.05) had a lower number <strong>of</strong> children ever born than their<br />
counterparts.Conclusion: The average number <strong>of</strong> children ever born is high among women in Nepal. There are many<br />
contributing factors for the high fertility, among which are age at first marriage, perceived ideal number <strong>of</strong> children, literacy<br />
status, mass media exposure, wealth status, and child-death experience by mothers. All <strong>of</strong> these were strong predictors for<br />
CEB. It can be concluded that programs should aim to reduce fertility rates by focusing on these identified factors so that<br />
fertility as well as infant and maternal mortality and morbidity will be decreased and the overall well-being <strong>of</strong> the family<br />
maintained and enhanced. © <strong>2010</strong> Adhikari; licensee BioMed Central Ltd.<br />
No.5<br />
Author (s) : Adhikari, R., Podhisita, C.<br />
Title : Household headship and child death: Evidence from Nepal<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Source : BMC <strong>International</strong> Health and Human Rights, Volume 10, Issue 1, <strong>2010</strong>, Article number 13<br />
Document Type : Article<br />
Keywords : adolescent, adult, article, birth, chi square test, child, child death, childhood mortality, demography, economic aspect, family,<br />
family planning, female, groups by age, health care facility, health care utilization, health survey, household, human, male,<br />
mother, Nepal, pregnancy, prenatal care, prevalence, reading, religion, social status, wellbeing<br />
<strong>Abstract</strong> : Background. Nepal has seen substantial improvements in its reproductive health outcomes, but infant and child mortality are<br />
still high. This study attempts to examine the prevalence and factors influencing the experience <strong>of</strong> child death <strong>of</strong> mothers<br />
who have given birth during a five-year period. More specifically, this paper aims to investigate whether household headship<br />
has an impact on child death in Nepal. Methods. This paper reports on data drawn from the Nepal Demographic and Health<br />
Survey (NDHS 2006), a nationally representative sample survey. The analysis is confined to women who had given birth during<br />
the five years preceding the survey (n = 4066). The association between experience <strong>of</strong> child death <strong>of</strong> mother and the<br />
explanatory variables was assessed via bivariate analysis using a chi-square test. The variables were also examined using<br />
multivariate analysis (binary logistic regression) to assess the net effect <strong>of</strong> household headship on child death after controlling<br />
for the other variables. Results. Out <strong>of</strong> all the mothers who had given birth during a five-years period, 3,229 (79.4%) were<br />
from male-headed households; the remaining 837 (20.6%) were from female-headed households. A significantly higher<br />
proportion <strong>of</strong> mothers from male-headed households (6.5%) than female-headed households (4.5%) had experienced the<br />
death <strong>of</strong> a child over the five years preceding the survey. Several socio-demographic, economic, and cultural variables were<br />
significant predicators for death <strong>of</strong> a child. For instance, women who had given birth to three or more children and who were<br />
Hindu were more likely to experience a child's death than were their counterparts. On the other hand, women who were<br />
literate, who had ever used family planning methods, who had visited a health facility, who utilized antenatal care for the<br />
last pregnancy, and who were from female-headed households were less likely to see a child die than were women in their<br />
comparison group. Notably, keeping all other control variables constant in the logistic model, women from female-headed<br />
households were 31 percent less likely to experience the death <strong>of</strong> a child (odds ratio = 0.69) than were women from maleheaded<br />
households. Conclusion. The death <strong>of</strong> children is not uncommon in Nepal. No single factor can account for the high<br />
child mortality in the country; many factors contribute to the problem. After controlling for other variables, this study found<br />
that, among many other factors, household headship was a strong predictor. Programs seeking to help remedy this problem<br />
should focus on the issues identified here regarding women's autonomy, such as reducing the number <strong>of</strong> children born,<br />
increasing women's literacy status, increasing the use <strong>of</strong> family planning, increasing the use <strong>of</strong> antenatal care, and increasing<br />
female household headship so that child mortality will decrease and the overall well-being <strong>of</strong> the family can be maintained<br />
and enhance. © <strong>2010</strong> Ramesh and Chai; licensee BioMed Central Ltd.<br />
No.6<br />
Author (s) : Adirekkiat, S., Sumethkul, V., Ingsathit, A., Domrongkitchaiporn, S., Phakdeekitcharoen, B., Kantachuvesiri, S., Kitiyakara, C.,<br />
Klyprayong, P., Disthabanchong, S.<br />
Title : Sodium thiosulfate delays the progression <strong>of</strong> coronary artery calcification in haemodialysis patients<br />
Source : Nephrology Dialysis Transplantation, Volume 25, Issue 6, June <strong>2010</strong>, Pages 1923-1929<br />
Document Type : Article<br />
Keywords : Bone; Haemodialysis; Sodium thiosulfate; Treatment; Vascular calcification<br />
<strong>Abstract</strong> : Background. Coronary artery calcification (CAC) is prevalent among haemodialysis patients and predicts cardiovascular<br />
mortality. In addition to modifying traditional cardiovascular risk factors, therapy aimed at lowering serum phosphate and<br />
calcium-phosphate product has been advocated. Sodium thiosulfate, through its chelating property, removes calcium from<br />
precipitated minerals decreasing calcification burden in calcific uraemic arteriolopathy and s<strong>of</strong>t tissue calcification. The effect<br />
<strong>of</strong> sodium thiosulfate on CAC in haemodialysis patients has never been studied.Methods. Eighty-seven stable chronic<br />
haemodialysis patients underwent multi-row spiral computed tomography and bone mineral density (BMD) measurement.<br />
Patients with a CAC score ≥300 were included to receive intravenous sodium thiosulfate infusion twice weekly posthaemodialysis<br />
for 4 months. CAC and BMD were re-evaluated at the end <strong>of</strong> the treatment course.Results. Progression <strong>of</strong> CAC<br />
occurred in 25% and 63% <strong>of</strong> the patients in the treatment and control group, respectively (P = 0.03). CAC score was<br />
unchanged in the treatment group but increased significantly in the control group. BMD <strong>of</strong> the total hip declined significantly<br />
in the treatment group. In multivariate analysis adjusted for factors that influenced CAC progression, therapy with sodium<br />
thiosulfate was an independent protective factor (odds ratio = 0.05, P = 0.04). Major side effects were persistent anorexia and<br />
metabolic acidosis.Conclusions. The effect <strong>of</strong> sodium thiosulfate in delaying the progression <strong>of</strong> CAC is encouraging and will<br />
3
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
require a larger study. Determination <strong>of</strong> the safe therapeutic window is necessary in order to avoid bone demineralization. ©<br />
<strong>2010</strong> The Author.<br />
No.7<br />
Author (s) : Afadlal, S., Polaboon, N., Surakul, P., Govitrapong, P., Jutapakdeegul, N.<br />
Title : Prenatal stress alters presynaptic marker proteins in the hippocampus <strong>of</strong> rat pups<br />
Source : Neuroscience Letters, Volume 470, Issue 1, 5 February <strong>2010</strong>, Pages 24-27<br />
Document Type : Article<br />
Keywords : Corticosterone; GAP-43; Hippocampus; pGAP-43; Prenatal stress; Synaptophysin<br />
<strong>Abstract</strong> : Exposure to stress during critical periods <strong>of</strong> an organism's maturation can result in permanent behavioral changes and induced<br />
hyper-responsive to aversive stimuli as adult. Hippocampus is a plastic and vulnerable brain structure that is susceptible to<br />
damage during aging and repeated stress. The present study examines the effect <strong>of</strong> maternal restraint stress on the level <strong>of</strong><br />
GAP-43, pGAP-43 and synaptophysin in the hippocampus <strong>of</strong> rat pups. Prenatal stress (PS) causes a significant increase <strong>of</strong> GAP-<br />
43 and pGAP-43 (p ≤ 0.001) in the pup's hippocampus during postnatal days 7 and 14, but not at later ages. Up-regulation <strong>of</strong><br />
GAP-43 and pGAP-43 may alter the pattern <strong>of</strong> axonal growth and synapses' formation in the pup's brain since the first two<br />
postnatal weeks are correlated with peak period <strong>of</strong> synaptogenesis in the rat brain. We also examined the level <strong>of</strong><br />
synaptophysin, a synaptic vesicle membrane protein, in the pup's brain. Our finding revealed that, PS causes a significant<br />
decrease <strong>of</strong> synaptophysin in the pup's hippocampus as compared to control (p ≤ 0.001). These changes are due to the<br />
direct effects <strong>of</strong> maternal stress hormone since repeated injection with corticosterone (CORT, 40 mg/kg) to pregnant rat during<br />
gestation days (GDs) 14-21 also gave the same results. Abnormal axonal sprouting and reorganization together with the<br />
alterations in synaptic vesicle membrane protein during the critical period <strong>of</strong> synaptogenesis may lead to a defect in synapse<br />
formation and axonal pruning in the hippocampus. These changes may be associated with stress-induced impairment <strong>of</strong><br />
hippocampal function that occurs in later life <strong>of</strong> the <strong>of</strong>fspring. © <strong>2010</strong> Elsevier Ireland Ltd. All rights reserved.<br />
No.8<br />
Author (s) : Aimyong, N.<br />
Title : FACTOR RELATED TO STATE ANXIETY OF SECONDARY SCHOOL STUDENT IN BANGKOK, THAILAND<br />
Source : INTERNATIONAL JOURNAL OF BEHAVIORAL MEDICINE, Volume 17, Pages 45-45, Supplement 1, AUG <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.9<br />
Author (s) : Akaraviputh, T., Arunakul, S., Lohsiriwat, V., Iramaneerat, C., Trakarnsanga, A.<br />
Title : Surgery for gastrointestinal malignant melanoma: Experience from surgical training center<br />
Source : World Journal <strong>of</strong> Gastroenterology, Volume 16, Issue 6, 14 February <strong>2010</strong>, Pages 745-748<br />
Document Type : Article<br />
Keywords : Gastrointestinal tract; Melanoma; Neoplasm metastasis<br />
<strong>Abstract</strong> : AIM: To characterize clinical features, surgery, outcome, and survival <strong>of</strong> malignant melanoma (MM) <strong>of</strong> the gastrointestinal (GI)<br />
tract in a surgical training center in Bangkok, Thailand. METHODS: A retrospective review was performed for all patients with<br />
MM <strong>of</strong> the GI tract treated at our institution between 1997 and 2007. RESULTS: Fourteen patients had GI involvement either<br />
in a metastatic form or as a primary melanoma. Thirteen patients with sufficient data were reviewed. The median age <strong>of</strong> the<br />
patients was 66 years (range: 32-87 years). Ten patients were female and three were male. Seven patients had primary<br />
melanomas <strong>of</strong> the anal canal, stomach and the sigmoid colon (5, 1 and 1 cases, respectively). Seven patients underwent<br />
curative resections: three abdominoperineal resections, two wide local excisions, one total gastrectomy and one<br />
sigmoidectomy. Six patients had distant metastatic lesions at the time <strong>of</strong> diagnosis, which made curative resection an<br />
inappropriate choice. Patients who underwent curative resection exhibited a longer mean survival time (29.7 mo, range: 10-96<br />
mo) than did patients in the palliative group (4.8 mo, P = 0.0006). CONCLUSION: GI MM had an unfavorable prognosis, except<br />
in patients who underwent curative resection (53.8% <strong>of</strong> cases), who had a mean survival <strong>of</strong> 29.7 mo. © <strong>2010</strong> Baishideng. All<br />
rights reserved.<br />
4
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.10<br />
Author (s) : Akaraviputh, T., Trakarnsanga, A., Suksamanapun, N.<br />
Title : Robot-assisted complete excision <strong>of</strong> choledochal cyst type I, hepaticojejunostomy and extracorporeal Roux-en-y anastomosis:<br />
A case report and review literature<br />
Source : World Journal <strong>of</strong> Surgical Oncology, Volume 8, 12 October <strong>2010</strong>, Article number 87<br />
Document Type : Article<br />
Keywords : abdominal surgery, adolescent, article, case report, common bile duct cyst, excision, extracorporeal roux en y<br />
anastomosis, female, follow up, hepatojejunostomy, human, robotics, cholecystectomy, computer assisted<br />
tomography, hepatic duct, jejunostomy, methodology, Roux Y anastomosis; Adolescent, Anastomosis, Roux-en-<br />
Y, Cholecystectomy, Laparoscopic, Choledochal Cyst, Female, Follow-Up Studies, Hepatic Duct,<br />
Common, Humans, Jejunostomy, Robotics, Tomography, X-Ray Computed<br />
<strong>Abstract</strong> : For Choledochal cyst type I, complete excision <strong>of</strong> cyst with Roux-en-Y hepaticojejunostomy anastomosis is the treatment <strong>of</strong><br />
choice. It has been performed laparoscopically with the advancement <strong>of</strong> laparoscopic skill. Recently, a telemanipulative<br />
robotic surgical system was introduced, providing laparoscopic instruments with wrist-arm technology and 3-dimensional<br />
visualization <strong>of</strong> the operative field. We present a case <strong>of</strong> robot-assisted total excision <strong>of</strong> a choledochal cyst type I and biliary<br />
reconstruction in a 14-year-old girl. No intraoperative complications or technical problems were encountered. An<br />
intraabdominal collection occurred and was successfully treated with continuous percutaneous drainage. At one-year followup,<br />
she is doing well without evidence <strong>of</strong> recurrent cholangitis. © <strong>2010</strong> Akaraviputh et al; licensee BioMed Central Ltd.<br />
No.11<br />
Author (s) : Ali, S., Najmi, M.H., Tarning, J., Lindegardh, N.<br />
Title : Pharmacokinetics <strong>of</strong> artemether and dihydroartemisinin in healthy Pakistani male volunteers treated with artemetherlumefantrine<br />
Source : Malaria Journal, Volume 9, Issue 1, <strong>2010</strong>, Article number 275<br />
Document Type : Article<br />
Keywords : artemether, artemether plus benflumetol, dihydroartemisinin, exafal, unclassified drug, antimalarial agent, artemisinin<br />
derivative, ethanolamine derivative, fluorene derivative; adult, area under the curve, article, drug blood level, drug<br />
clearance, drug distribution, drug half life, human, liquid chromatography, male, Pakistan, single drug dose, solid phase<br />
extraction, tandem mass spectrometry, chemistry, drug combination, human experiment, middle aged, oral drug<br />
administration, plasma, time; Administration, Oral, Adult, Antimalarials, Artemisinins, Chromatography, Liquid, Drug<br />
Combinations, Ethanolamines, Fluorenes, Human Experimentation, Humans, Male, Middle Aged, Pakistan, Plasma, Tandem<br />
Mass Spectrometry, Time Factors<br />
<strong>Abstract</strong> : Background. Artemether-lumefantrine is one <strong>of</strong> the most widely used anti-malarial drug combinations in the world with<br />
excellent tolerability and cure rates in adult and paediatric patients with uncomplicated falciparum malaria. The aim <strong>of</strong> this<br />
study was to evaluate the pharmacokinetics <strong>of</strong> artemether and its active metabolite, dihydroartemisinin, in healthy Pakistani<br />
volunteers. Methods. Twelve healthy male Pakistani subjects, aged 20 to 50, were recruited into the study. A fixed oral<br />
combination <strong>of</strong> artemether-lumefantrine (80-480 mg) was given as a single oral dose. Frequent blood samples were collected<br />
and artemether and dihydroartemisinin were quantified in human plasma using solid-phase extraction and liquid<br />
chromatography coupled with tandem mass spectrometry. Drug concentration-time data were evaluated with noncompartmental<br />
analysis. Results. Observed maximum concentrations (mean SD) <strong>of</strong> artemether and dihydroartemisinin were<br />
184 100 ng/mL and 126 46 ng/mL, respectively. These concentrations were reached at 1.56 0.68 hr and 1.69 0.59 hr,<br />
respectively, after drug intake. The terminal elimination half-life <strong>of</strong> artemether and dihydroartemisinin were 2.00 0.71 hr and<br />
1.80 0.31 hr, respectively. Apparent volume <strong>of</strong> distribution and oral clearance for artemether were estimated to 666 220 L<br />
and 257 140 L/hr. The same parameters were estimated to 702 220 L and 269 57 L/hr for dihydroartemisinin. Conclusions.<br />
The overall pharmacokinetic properties <strong>of</strong> artemether and dihydroartemisinin in healthy Pakistani subjects are comparable to<br />
healthy subjects and patients from other populations. © <strong>2010</strong> Ali et al; licensee BioMed Central Ltd.<br />
No.12<br />
Author (s) : Allen, M.A.<br />
Title : On the current obsession with publication statistics<br />
Source : ScienceAsia, Volume 36, Issue 1, March <strong>2010</strong>, Pages 1-5<br />
Document Type : Article<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : Bibliometrics; Citations; H-index; Impact factor; Research assessment<br />
<strong>Abstract</strong> : Crude publication statistics such as publication counts and impact factors are routinely being employed to assess individuals<br />
and institutions. Although they can play a role in an approximate preliminary assessment, using them for anything more is<br />
inappropriate due to their over-simplicity and ease <strong>of</strong> manipulation. Furthermore, it is argued that rewarding scientists for<br />
achieving high scores in such number-based evaluations ultimately leads to a slowing <strong>of</strong> scientific progress. Suggestions are<br />
given on how reliance on statistics can be reduced and their manipulation discouraged.<br />
No.13<br />
Author (s) : Amnuaypornsri, S., Sakdapipanich, J., Tanaka, Y.<br />
Title : Highly purified natural rubber by saponificaion <strong>of</strong> latex: Analysis <strong>of</strong> green and cured properties<br />
Source : Journal <strong>of</strong> Applied Polymer Science, Volume 118, Issue 6, 15 December <strong>2010</strong>, Pages 3524-3531<br />
Document Type : Article<br />
Keywords : Dynamic mechanical properties; Natural rubber; Physical properties; Purification; Saponification<br />
<strong>Abstract</strong> : Natural rubber (NR) was purified by saponification <strong>of</strong> fresh latex from Hevea brasiliensis and soaking process <strong>of</strong> the coagulum<br />
with aqueous NaOH solution. This treatment resulted in the decrease <strong>of</strong> nitrogen content to the same level as enzymatic<br />
deproteinized NR. The saponification <strong>of</strong> NR latex and soaking <strong>of</strong> the coagulum gave the rubber having good processability<br />
with easy to cure. The saponified rubber showed outstanding physical and dynamic mechanical properties such as high<br />
tensile properties, high storage modulus, low tan δ, low heat build-up and low dynamic compression set. The saponified<br />
rubber blend with styrene butadiene rubber (SBR) according to the recipe <strong>of</strong> rubber tire showed also a good performance. ©<br />
<strong>2010</strong> Wiley Periodicals, Inc.<br />
No.14<br />
Author (s) : Amnuaypornsri, S., Tarachiwin, L., Sakdapipanich, J.T.<br />
Title : Character <strong>of</strong> long-chain branching in highly purified natural rubber<br />
Source : Journal <strong>of</strong> Applied Polymer Science, Volume 115, Issue 6, 15 March <strong>2010</strong>, Pages 3645-3650<br />
Document Type : Article<br />
Keywords : Networks; Rubber; Structure<br />
<strong>Abstract</strong> : The nature <strong>of</strong> long-chain branching in natural rubber (NR) from Hevea brasiliensis was analyzed for NR purified by enzymatic<br />
deproteinization in the latex state followed by acetone extraction in the solid state to remove the proteins and neutral lipids,<br />
respectively. The treatment <strong>of</strong> purified NR in a toluene solution with a polar solvent, such as methanol or acetic acid,<br />
resulted in a clear decrease in the molecular weight, gel content, and Huggins' constant; this was caused by the<br />
decomposition <strong>of</strong> branch points in the purified rubber. This finding clearly showed that long-chain branching in the purified<br />
NR was mainly derived from the association <strong>of</strong> phospholipids linked with both terminal groups in the rubber chain via<br />
hydrogen bonds. © 2009 Wiley Periodicals, Inc.<br />
No.15<br />
Author (s) : Amonruttanapun, P., Chongthammakun, S.<br />
Title : EXPRESSION OF TRIGGERING RECEPTOR EXPRESSED ON MYELOID CELLS-2 (TREM-2) ON HIGHLY AGGRESSIVELY PROLIFERATING<br />
IMMORTALIZED (HAPI) CELLS IN RECOGNITION OF APOPTOTIC NEURONS<br />
Source : JOURNAL OF NEUROCHEMISTRY Volume: 115 Pages: 44-45 Supplement: 1 Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.16<br />
Author (s) : Amornsakchai, T., Pattarachindanuwong, S.<br />
Title : Surface grafting <strong>of</strong> polyethylene fiber for improved adhesion to acrylic resin<br />
Source : Journal <strong>of</strong> Reinforced Plastics and Composites, Volume 29, Issue 1, January <strong>2010</strong>, Pages 149-158<br />
Document Type : Article<br />
Keywords : Adhesion; Polyethylene fiber; Surface grafting<br />
<strong>Abstract</strong> : Surface grafting <strong>of</strong> polyethylene (PE) fiber with methyl methacrylate (MMA) and acrylic acid (AAc) were prepared with twostage<br />
method. Functionalization was first carried out in aqueous solution <strong>of</strong> potassium peroxydisulfate at 80°C. The fiber was<br />
6
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
then surface grafted with MMA and AAc. Single fiber composite <strong>of</strong> grafted fibers/Orthocryl® acrylic resin were prepared and<br />
adhesion evaluated with pull-out test. Acrylic matrix was prepared in two methods. In the first method, acrylic powder was<br />
bonded together with acetone. In the second, the powder was mixed with liquid monomer to form self-cure material. It was<br />
found that in the former case, in which only interdiffusion <strong>of</strong> polymer chains can occur, MMA-modified polyethylene fiber had<br />
slightly higher pull-out strength than AAc-modified fiber. In the latter case, in which chemical reaction occurred, AAc-modified<br />
fiber provided much higher pull-out strength than MMA-modified fiber. The difference in adhesion level is discussed. © <strong>2010</strong><br />
SAGE <strong>Publications</strong>.<br />
No.17<br />
Author (s) : Amornsamankul, S., Kaorapapong, K., Wiwatanapataphee, B.<br />
Title : Three-dimensional simulation <strong>of</strong> femur bone and implant in femoral canal using finite element method<br />
Source : <strong>International</strong> Journal <strong>of</strong> Mathematics and Computers in Simulation, Volume 4, Issue 4, <strong>2010</strong>, Pages 171-178<br />
Document Type : Article<br />
Keywords : Femur bone; Finite element method; Implant in the femoral canal; Mathematical modeling; Three dimensional simulation<br />
<strong>Abstract</strong> : In this paper, a mathematical model is developed to simulate three-dimensional femur bone and femur bone with implant in<br />
the femoral canal, taking into account stress distribution and totaldisplacement during horizontal walking. The equilibrium<br />
equationsare used in the model. Realistic domain are created by using CT scan data. Different cases <strong>of</strong> static loads and<br />
different boundary conditions are used in the simulation. The finite element method is utilized to determine total<br />
displacement and Von Mises Stress. The influences <strong>of</strong> human weight during horizontal walking are investigated. This model<br />
will give the useful for surgeon in femur surgeries. The results show that higher weight provides higher total displacement.<br />
And it is found that the Von Mises stress affects the lateral femur.<br />
No.18<br />
Author (s) : Amornsamankul, S., Kraipeerapun, P.<br />
Title : Bagging <strong>of</strong> duo output neural networks for single output regression problem<br />
Source : Proceedings - <strong>2010</strong> 3rd IEEE <strong>International</strong> Conference on Computer Science and Information Technology, ICCSIT <strong>2010</strong>, Volume<br />
7, <strong>2010</strong>, Article number 5564576, Pages 135-139<br />
Document Type : Conference Paper<br />
Keywords : Backpropagation neural network; Ensemble neural network; Regression problem<br />
<strong>Abstract</strong> : This paper presents an approach to the single output regression problem using ensemble <strong>of</strong> duo output neural networks<br />
based on bagging technique. Each component in the ensemble consists <strong>of</strong> a pair <strong>of</strong> duo output neural networks. The first<br />
neural network is trained to provide duo outputs which are a pair <strong>of</strong> truth and falsity values whereas the second neural<br />
network provides a pair <strong>of</strong> falsity and truth values. The target outputs used to train the second network are organized in<br />
reverse order <strong>of</strong> the first network. For the former neural network, the truth and non-falsity outputs are used to created the<br />
average truth output. For the later neural network, the falsity and non-truth outputs are used to provide the average falsity<br />
output. In order to combine outputs from components in the ensemble, the simple averaging and the dynamic weighted<br />
averaging techniques are used. The weight is created based on the difference between the truth and non-falsity values. The<br />
proposed approach has been tested with three benchmarking VCI data sets, which are housing, concrete compressive<br />
strength, and computer hardware. The proposed ensemble methods improves the performance as compared to the<br />
traditional ensemble <strong>of</strong> neural networks, the ensemble <strong>of</strong> complementary neural networks, and the ensemble <strong>of</strong> support<br />
vector machine with linear, polynomial, and radial basis function kernels. © <strong>2010</strong> IEEE.<br />
No.19<br />
Author (s) : Amornsamankul, S., Wiwatanapataphee, B., Kaorapapong, K.<br />
Title : Three-dimensional simulation <strong>of</strong> the femur bone using finite element method<br />
Source : <strong>International</strong> Conference on Applied Computer Science - Proceedings, <strong>2010</strong>, Pages 304-307<br />
Document Type : Conference Paper<br />
Keywords : Femur bone; Finite element method; Mathematical modeling; Simulation; Total displacement<br />
<strong>Abstract</strong> : In this paper, a mathematical model is developed to simulate three-dimensional femur bone, taking into account the stress<br />
distribution and total displacement during horizontal walking. The principles <strong>of</strong> continuum mechanics, the field equations<br />
governing the displacement and stress fields in the femur bone including the stress equilibrium equations are used in the<br />
model. Realistic domain is created using CT scan data. Three different cases <strong>of</strong> static loads and three boundary conditions are<br />
7
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
used in the simulation. The finite element method is utilized to determine the total displacement and the Von Misses stress.<br />
The influences <strong>of</strong> human weight during horizontal walking are investigated. This model provides useful information for<br />
surgeons involved with femur surgeries.<br />
No.20<br />
Author (s) : Amornyotin, S., Aanpreung, P.<br />
Title : Clinical effectiveness <strong>of</strong> an anesthesiologist-administered intravenous sedation outside <strong>of</strong> the main operating room for<br />
pediatric upper gastrointestinal endoscopy in Thailand<br />
Source : <strong>International</strong> Journal <strong>of</strong> Pediatrics, Volume <strong>2010</strong>, Article ID 748564, 6 pages<br />
Document Type : Article<br />
Keywords : –<br />
<strong>Abstract</strong> : Objectives. To review our sedation practice and to evaluate the clinical effectiveness <strong>of</strong> an anesthesiologist-administered<br />
intravenous sedation outside <strong>of</strong> the main operating room for pediatric upper gastrointestinal endoscopy (UGIE) in Thailand.<br />
Subjects and Methods. We undertook a retrospective review <strong>of</strong> the sedation service records <strong>of</strong> pediatric patients who<br />
underwent UGIE. All endoscopies were performed by a pediatric gastroenterologist. All sedation was administered by staff<br />
anesthesiologist or anesthetic personnel. Results. A total <strong>of</strong> 168 patients (94 boys and 74 girls), with age from 4 months to 12<br />
years, underwent 176 UGIE procedures. Of these, 142 UGIE procedures were performed with intravenous sedation (IVS). The<br />
mean sedation time was 23.2 +/- 10.0 minutes. Prop<strong>of</strong>ol was the most common sedative drugs used. Mean dose <strong>of</strong> prop<strong>of</strong>ol,<br />
midazolam and fentanyl was 10.0 +/- 7.5 mg/kg/hr, 0.2 +/- 0.2 mg/kg/hr, and 2.5 +/- 1.2 mcg/kg/hr, respectively.<br />
Complications relatively occurred frequently. All sedations were successful. However, two patients became more deeply than<br />
intended and required unplanned endotracheal intubation. Conclusion. The study shows the clinical effectiveness <strong>of</strong> an<br />
anesthesiologist-administered IVS outside <strong>of</strong> the main operating room for pediatric UGIE in Thailand. All complications are<br />
relatively high. We recommend the use <strong>of</strong> more sensitive equipments such as end tidal CO(2) and carefully select more<br />
appropriate patients.<br />
No.21<br />
Author (s) : Amornyotin, S., Chalayonnavin, W., Kongphlay, S.<br />
Title : Assisted sedation for percutaneous endoscopic gastrostomy procedure in sick patients in a developing country<br />
Source : Gastroenterology Insights, Volume 2, Issue 1, <strong>2010</strong>, Pages 17-20<br />
Document Type : Article<br />
Keywords : General anesthesia; Intravenous sedation; Percutaneous endoscopic gastrostomy<br />
<strong>Abstract</strong> : In a developing country, the percutaneous endoscopic gastrostomy (PEG) procedure is being performed at an increasing rate.<br />
However, there is sparse information on the sedation for this procedure. The aim <strong>of</strong> our study was to evaluate the clinical<br />
efficacy <strong>of</strong> assisted sedation compared with general anesthesia for the PEG procedure in patients in a teaching hospital in<br />
Thailand. We undertook a retrospective review <strong>of</strong> records <strong>of</strong> patients who underwent PEG procedures from October 2006 to<br />
September 2008. AH patients with ASA physical status III and IV were classified into two groups according to the type <strong>of</strong><br />
anesthetic technique: Group S, intravenous sedation (IVS); and Group G, general anesthesia (GA). The primary outcome <strong>of</strong> the<br />
study was the successful completion <strong>of</strong> the procedure. The secondary outcomes were sedation/anesthesia-related<br />
complications during and immediately after the procedure. One hundred and eighty-four patients were enrolled with 116 (M,<br />
52; mean age, 71.4±15.8 years) in Group S and 68 (M, 37; mean age, 67.8±18.5 years) in Group G. There were no significant<br />
differences between the two groups in age, gender, weight, and duration <strong>of</strong> procedure. AH patients in both groups concluded<br />
with the successful completion <strong>of</strong> the procedure. Overall adverse events in group S occurred significantly less commonly<br />
than in group G (P=0.003). AH complications were treated easily with no adverse sequelae. We concluded that in the setting<br />
<strong>of</strong> the developing country, assisted sedation for the PEG procedure in patients by trained anesthetic personnel with<br />
appropriate monitoring was safe and effective. © S. Amornyotin et al., <strong>2010</strong>.<br />
No.22<br />
Author (s) : Amornyotin, S., Prakanrattana, U., Kachintorn, U., Chalayonnavin, W., Kongphlay, S.<br />
Title : Prop<strong>of</strong>ol-based sedation does not increase rate <strong>of</strong> perforation during colonoscopic procedure<br />
Source : Gastroenterology Insights, Volume 2, Issue 1, <strong>2010</strong>, Pages 13-16<br />
Document Type : Article<br />
Keywords : Colonoscopy; Intravenous sedation; Perforation; Prop<strong>of</strong>ol<br />
8
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : Sedation-related colonoscopic perforation (CP) has been under much debate. Our aim was to assess and compare the CP<br />
rate during colonoscopy by using sedation with or without prop<strong>of</strong>ol adjuvant. All patients who underwent colonoscopic<br />
procedure at the WGO Endoscopy Training Center, Siriraj Hospital, Thailand from March 2005 to October 2007 by using the<br />
intravenous sedation (IVS) technique were analyzed. The primary outcome was the CP rate; the secondary outcomes were<br />
sedation-related complications and death during and immediately after the procedure. There were 6140 colonoscopies and<br />
1532 flexible sigmoidoscopies during the study period, <strong>of</strong> which 6122 colonoscopic procedures were performed by using IVS.<br />
All <strong>of</strong> these procedures were categorized into two groups: group A, the IVS technique was prop<strong>of</strong>ol-based sedation and group<br />
B, the IVS technique was non-prop<strong>of</strong>ol-based sedation. After matching the indications <strong>of</strong> procedure, there were 2022<br />
colonoscopies in group A and 512 colonoscopies in group B. Colonoscopic procedures were performed by staff endoscopists<br />
(10.8%) or residents and fellows (89.2%). The characteristics <strong>of</strong> patients and sedative agents used in perforated patients in<br />
both groups were not significantly different. In group A, five patients (0.25%) suffered from perforation and two <strong>of</strong> them died.<br />
In group B, one patient (0.20%) had CP; the difference was not significant (P=0.829). Our data showed that colonoscopy under<br />
prop<strong>of</strong>ol-based sedation did not increase the perforation rate. Serious complications are uncommon. © S. Amornyotin et al.,<br />
<strong>2010</strong>.<br />
No.23<br />
Author (s) : Amornyotin, S., Prakanrattana, U., Vichitvejpaisal, P., Vallisut, T., Kunanont, N., Permpholprasert, L.<br />
Title : Comparison <strong>of</strong> the Clinical Use <strong>of</strong> Macintosh and Miller Laryngoscopes for Orotracheal Intubation by Second-Month Nurse<br />
Students in Anesthesiology<br />
Source : Anesthesiology Research and Practice, Volume <strong>2010</strong>, <strong>2010</strong>, Article number 432846<br />
Document Type : Article<br />
Keywords : adult, article, bleeding, clinical trial, controlled clinical trial, controlled study, endotracheal intubation, feasibility<br />
study, female, heart rate, human, intermethod comparison, laryngoscope, major clinical study, male, mean arterial<br />
pressure, mouth injury, nursing student, patient safety, priority journal, randomized controlled trial, therapy effect, tooth<br />
injury, treatment outcome<br />
<strong>Abstract</strong> : Aim. The aim <strong>of</strong> this study is to compare the clinical feasibility <strong>of</strong> Macintosh and Miller laryngoscopes for tracheal intubation<br />
in non-experienced users in anesthetized patients. Patients and Methods. 119 patients were randomized into the Macintosh<br />
group (59) and the Miller group (60). The primary outcome variable was successful tracheal intubation. The secondary<br />
outcome variables were number <strong>of</strong> insertion attempt, intubation time needed, total time to intubation, hemodynamic change<br />
and complications. Results. All patients were successfully intubated using the Macintosh, whereas 13 patients (21.6) were<br />
failed with the Miller (P
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : Cytotoxicity; Latex; Nanoparticles; Rubber; Surface modification<br />
<strong>Abstract</strong> : In order to increase surface roughness <strong>of</strong> the sulphur-prevulcanized natural rubber (SPNR) film and, hence, decrease the<br />
direct contact between the rubber and skin, the poly(methyl methacrylate) (PMMA) latex particles were deposited onto the<br />
SPNR film grafted with polyacrylamide (SPNR-g-PAAm). The surface coverage <strong>of</strong> PMMA particles on the SPNR-g-PAAm increased<br />
with increasing latex immersion time, particle size and concentration. Prior to the in vitro cytotoxicity evaluation on L-929<br />
fibroblasts, the SPNR and SPNR-g-PAAm coated with PMMA particles were extracted by using the culture medium. Results<br />
showed that the cytotoxicity effect could be significantly reduced by coating PMMA particles onto the rubber film. At the<br />
extract concentrations <strong>of</strong> ≤12.5% for 24. h at 37. °C, no toxicity potential was detected. The study will be helpful for<br />
development <strong>of</strong> gloves designed for the hypersensitive person. © <strong>2010</strong> Elsevier B.V.<br />
No.26<br />
Author (s) : Anancharungsuk, W., Taweepreda, W., Wirasate, S., Thonggoom, R., Tangboriboonrat, P.<br />
Title : Reduction <strong>of</strong> surface friction <strong>of</strong> natural rubber film coated with PMMA particle: Effect <strong>of</strong> particle size<br />
Source : Journal <strong>of</strong> Applied Polymer Science, Volume 115, Issue 6, 15 March <strong>2010</strong>, Pages 3680-3686<br />
Document Type : Article<br />
Keywords : Emulsion polymerization; Films; Rubber; Selfassembly; Surfaces<br />
<strong>Abstract</strong> : The friction coefficient <strong>of</strong> the sulphurprevulcanized natural rubber (SPNR) film could be effectively reduced by deposition <strong>of</strong><br />
poly(methyl methacrylate) (PMMA) particles. The nanoscale surface roughness <strong>of</strong> rubber, determined by atomic force<br />
microscope, was directly proportional to the particle size <strong>of</strong> PMMA particle at 12% surface coverage (Cs). The %Cs and surface<br />
roughness <strong>of</strong> the modified SPNR increased, while the friction coefficient decreased, with increasing PMMA latex concentration<br />
and immersion time. By using a mixture <strong>of</strong> latexes having both large and small sizes, the increase in the amount <strong>of</strong> small<br />
particles resulted in the better distribution <strong>of</strong> large particles deposited on the rubber surface. © 2009 Wiley Periodicals, Inc.<br />
No.27<br />
Author (s) : Ananchotikul, N., Kouwenberg, R., Phunnarungsi, V.<br />
Title : Do Firms Decouple Corporate Governance Policy and Practice?<br />
Source : European Financial Management, Volume 16, Issue 5, November <strong>2010</strong>, Pages 712-737<br />
Document Type : Article<br />
Keywords : cheap talk; corporate governance; fraud; G3; G38; K42; violations<br />
<strong>Abstract</strong> : We test whether Thai listed firms with higher levels <strong>of</strong> good governance policy adoption are less likely to violate listing rules<br />
and laws designed to protect shareholders. Our results suggest that firms on average implement, substantively as opposed to<br />
symbolically, recommended governance policies, as violations occur less frequently among firms with higher governance<br />
policy adoption scores. However, we also find evidence <strong>of</strong> symbolic governance among a small group <strong>of</strong> 'talk-only' firms that<br />
issue statements about governance while lagging in the adoption <strong>of</strong> policies related to shareholder rights and the board <strong>of</strong><br />
directors. © <strong>2010</strong> Blackwell Publishing Ltd.<br />
No.28<br />
Author (s) : Anantachaisilp, S., Smith, S.M., Treetong, A., Pratontep, S., Puttipipatkhachorn, S., Ruktanonchai, U.R.<br />
Title : Chemical and structural investigation <strong>of</strong> lipid nanoparticles: Drug-lipid interaction and molecular distribution<br />
Source : Nanotechnology, Volume 21, Issue 12, <strong>2010</strong>, Article number 125102<br />
Document Type : Article<br />
Keywords : Chemical retention, Crystallinities, Drug delivery system, High pressure homogenization, High resolution, Lipid content, Lipid<br />
nanoparticles, Medium chain triglycerides, Molecular distribution, Oryzanol, Phase-separated structures, Powder X ray<br />
diffraction, Proton nuclear magnetic resonance, Raman spectroscopic, Release characteristics, Simple model, Structural<br />
investigation; Atomic force microscopy, Drug delivery, Drug interactions, Indicators (chemical), Nanoparticles, Nuclear magnetic<br />
resonance, Nuclear magnetic resonance spectroscopy, Photon correlation spectroscopy, Raman spectroscopy, Resonance, X<br />
ray diffraction; Liquids; cetyl palmitate, drug carrier, gamma oryzanol, gamma-oryzanol, nanoparticle, palmitic acid<br />
derivative, phenylpropionic acid derivative, triacylglycerol; analysis <strong>of</strong> variance, area under the curve, article, atomic force<br />
microscopy, chemistry, drug delivery system, nuclear magnetic resonance, particle size, powder diffraction, Raman<br />
spectrometry, thermodynamics; Analysis <strong>of</strong> Variance, Area Under Curve, Drug Carriers, Drug Delivery Systems, Microscopy,<br />
Atomic Force, Nanoparticles, Nuclear Magnetic Resonance, Biomolecular, Palmitates, Particle Size, Phenylpropionates, Powder<br />
Diffraction, Spectrum Analysis, Raman, Thermodynamics, Triglycerides<br />
10
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : Lipid nanoparticles are a promising alternative to existing carriers in chemical or drug delivery systems. A key challenge is to<br />
determine how chemicals are incorporated and distributed inside nanoparticles, which assists in controlling chemical<br />
retention and release characteristics. This study reports the chemical and structural investigation <strong>of</strong> γ-oryzanol loading inside<br />
a model lipid nanoparticle drug delivery system composed <strong>of</strong> cetyl palmitate as solid lipid and Miglyol 8121 as liquid lipid.<br />
The lipid nanoparticles were prepared by high pressure homogenization at varying liquid lipid content, in comparison with the<br />
γ-oryzanol free systems. The size <strong>of</strong> the lipid nanoparticles, as measured by the photon correlation spectroscopy, was found<br />
to decrease with increased liquid lipid content from 200 to 160nm. High-resolution proton nuclear magnetic resonance (1H-<br />
NMR) measurements <strong>of</strong> the medium chain triglyceride <strong>of</strong> the liquid lipid has confirmed successful incorporation <strong>of</strong> the liquid<br />
lipid in the lipid nanoparticles. Differential scanning calorimetric and powder x-ray diffraction measurements provide<br />
complementary results to the 1H-NMR, whereby the crystallinity <strong>of</strong> the lipid nanoparticles diminishes with an increase in the<br />
liquid lipid content. For the distribution <strong>of</strong> γ-oryzanol inside the lipid nanoparticles, the 1H-NMR revealed that the chemical<br />
shifts <strong>of</strong> the liquid lipid in γ-oryzanol loaded systems were found at rather higher field than those in γ-oryzanol free systems,<br />
suggesting incorporation <strong>of</strong> γ-oryzanol in the liquid lipid. In addition, the phase-separated structure was observed by atomic<br />
force microscopy for lipid nanoparticles with 0% liquid lipid, but not for lipid nanoparticles with 5 and 10% liquid lipid.<br />
Raman spectroscopic and mapping measurements further revealed preferential incorporation <strong>of</strong> γ-oryzanol in the liquid part<br />
rather than the solid part <strong>of</strong> in the lipid nanoparticles. Simple models representing the distribution <strong>of</strong> γ-oryzanol and lipids<br />
(solid and liquid) inside the lipid nanoparticle systems are proposed. © <strong>2010</strong> IOP Publishing Ltd.<br />
No.29<br />
Author (s) : Anantamongkol, U., Charoenphandhu, N., Wongdee, K., Teerapornpuntakit, J., Suthiphongchai, T., Prapong, S., Krishnamra, N.<br />
Title : Transcriptome analysis <strong>of</strong> mammary tissues reveals complex patterns <strong>of</strong> transporter gene expression during pregnancy and<br />
lactation<br />
Source : Cell Biology <strong>International</strong>, Volume 34, Issue 1, January <strong>2010</strong>, Pages 67-74<br />
Document Type : Article<br />
Keywords : Canonical transient receptor potential (TRPC) channel; Gene ontology; MCF-7 cells; Microarray; Real-time PCR<br />
<strong>Abstract</strong> : As a complex Ca2+-rich fluid mixture <strong>of</strong> water, casein, lactose and several ions, milk secretion requires a number <strong>of</strong> unknown<br />
transporters, which can be identified by a genome-wide microarray study in mammary tissues <strong>of</strong> lactating animals. Ca2+ was<br />
reported to be secreted across mammary epithelial cells through the transcellular pathway, presumably involving TRPC<br />
(canonical transient receptor potential) channels. In the present study, we have used quantitative real-time PCR to<br />
demonstrate that the human mammary cell line MCF-7, as well as rat mammary tissues from pregnant and lactating rats,<br />
expressed TRPC1, TRPC5 and TRPC6. Expression <strong>of</strong> TRPC1, TRPC5 and TRPC7 were markedly up-regulated, whereas that <strong>of</strong><br />
TRPC3 and TRPC4 was down-regulated in the early lactating period. To further identify other transporter genes affected by<br />
lactation, a highly sensitive Illumina microarray featuring Bead Array technology was performed on RNA samples from<br />
mammary tissues <strong>of</strong> lactating rats. We found that, <strong>of</strong> the 384 transcripts changed during lactation, 31 transcripts were involved<br />
in the transport <strong>of</strong> water and electrolytes, such as Ca2+, Na+, K+, Cl-, I-, Fe2+, sulfate and phosphate. The present study,<br />
therefore, provides information for further investigation <strong>of</strong> the mechanism <strong>of</strong> lactationinduced transport adaptation in<br />
mammary epithelial cells. © The Author(s) Journal compilation. © <strong>2010</strong> Portland Press Ltd.<br />
No.30<br />
Author (s) : Anantaphruti, M.T., Koga, M., Nuamtanong, S., Nintasen, R.<br />
Title : Esophageal deformation <strong>of</strong> Gnathostoma spinigerum in ivermectin-treated rats, and anthelminthic efficacy<br />
Source : Helminthologia, Volume 47, Issue 2, June <strong>2010</strong>, Pages 88-93<br />
Document Type : Article<br />
Keywords : Esophagus tissues; Gnathostoma spinigerum; Ivermectin; Rats; Treatment efficacy<br />
<strong>Abstract</strong> : Ivermectin 2 mg/kg single dose reduced worm load by 83.3 % (75.0 - 91.7 %) for rats experimentally infected with 20<br />
Gnathostoma spinigerum advanced third-stage larvae administered at week 1, with sacrifice at week 15 post-infection. Some<br />
larvae recovered from the muscles <strong>of</strong> treated rats were found to be grossly deformed in appearance. Morphological changes<br />
in the Gnathostoma larvae were clearly observed. The internal structures <strong>of</strong> the deformed larvae showed significant<br />
degeneration in the esophageal glandular tissues. The esophageal lumen branched irregularly and occupied a wider area in<br />
the center <strong>of</strong> the esophagus. The epithelial cells thickened along these branches. The esophageal gland ducts were<br />
completely destroyed. The excretory ability <strong>of</strong> the esophageal cell glands may be depressed, causing loss <strong>of</strong> function. It is<br />
11
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
suggested that ivermectin is effective against Gnathostoma larvae due to worm recovery reductions. The drug efficacy appears<br />
to generate by esophageal destruction <strong>of</strong> the worms. © <strong>2010</strong> Versita Warsaw and Springer-Verlag Wien.<br />
No.31<br />
Author (s) : Anantaphruti, M.T., Okamoto, M., Yoonuan, T., Saguankiat, S., Kusolsuk, T., Sato, M., Sato, M.O., Sako, Y., Waikagul, J., Ito, A.<br />
Title : Molecular and serological survey on taeniasis and cysticercosis in Kanchanaburi Province, Thailand<br />
Source : Parasitology <strong>International</strong>, Volume 59, Issue 3, September <strong>2010</strong>, Pages 326-330<br />
Document Type : Article<br />
Keywords : Cysticercosis; ELISA; Immunoblot; Kanchanaburi, Thailand; Molecular diagnosis; Taeniasis<br />
<strong>Abstract</strong> : A community-based field survey on taeniasis and cysticercosis was performed in two villages in Thong Pha Phum District,<br />
Kanchanaburi Province, central Thailand, where 3 Taenia species, T. solium, T. saginata and T. asiatica, are sympatrically<br />
occurring. Four (0.6%) out <strong>of</strong> 667 stool samples were egg-positive for Taenia sp. by Kato-Katz technique. Three out <strong>of</strong> those<br />
four persons and other three persons who were Taenia egg-negative but having a recent (1 year) history <strong>of</strong> expelling proglottids in the<br />
stool. © <strong>2010</strong>.<br />
No.32<br />
Author (s) : Anantasit N., Kamchaisatian W., Direkwattanachai C., Manuyakorn W., Vilaiyuk S., Benjaponpitak S.<br />
Title : Aeroallergen Sensitization Among Children with Respiratory Allergy in Ramathibodi Hospital, Bangkok<br />
Source : JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, Volume: 125, Issue: 2, Supplement: 1, Pages: AB17-AB17, Published: FEB<br />
<strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.33<br />
Author (s) : Ananworanich, J., Apornpong, T., Kosalaraksa, P., Jaimulwong, T., Hansudewechakul, R., Pancharoen, C., Bunupuradah, T.,<br />
Chandara, M., Puthanakit, T., Ngampiyasakul, C., Wongsawat, J., Kanjanavanit, S., Luesomboon, W., Klangsinsirikul, P., Ngo-<br />
Giang-Huong, N., Kerr, S.J., Ubolyam, S., Mengthaisong, T., Gelman, R.S., Pattanapanyasat, K., Saphonn, V., Ruxrungtham, K.,<br />
Shearer, W.T.<br />
Title : Characteristics <strong>of</strong> lymphocyte subsets in HIV-infected, long-term nonprogressor, and healthy Asian children through 12 years<br />
<strong>of</strong> age<br />
Source : Journal <strong>of</strong> Allergy and Clinical Immunology, Volume 126, Issue 6, December <strong>2010</strong>, Pages 1294-1301.e1-e10<br />
Document Type : Article<br />
Keywords : antiretroviral therapy; Asia; children; disease progression; HIV; long-term<br />
nonprogressors; lymphocyte; monocyte; pediatric AIDS; phenotyping<br />
<strong>Abstract</strong> : Background: There are limited data on the immune pr<strong>of</strong>iles <strong>of</strong> HIV-positive children compared with healthy controls, and no<br />
such data for Asian children. Objectives: To immunophenotype HIV-positive Asian children, including long-term<br />
nonprogressors (LTNPs), compared with age-matched healthy controls. Methods: We used flow cytometry to analyze 13<br />
lymphocyte and monocyte subsets from 222 untreated, HIV-positive children with 15% to 24% CD4+ T cells and no AIDSrelated<br />
illnesses and 142 healthy children (controls). Data were compared among age categories. Pr<strong>of</strong>iles from LTNPs (n = 50),<br />
12
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
defined as children ≥8 years old with CD4+ T-cell counts ≥350 cells/mm3, were compared with data from age-matched non-<br />
LTNPs (n = 17) and controls (n = 53). Results: Compared with controls, HIV-positive children had lower values (cell count per<br />
mm3 and percent distribution) for TH cells and higher values for cytotoxic T cells, with reductions in populations <strong>of</strong> naive TH<br />
and cytotoxic T cells, B cells, and natural killer (NK) cells. HIV-positive children had high values for activated TH and cytotoxic<br />
T cells. Compared with non-LTNPs, LTNPs had higher values <strong>of</strong> TH and cytotoxic T cells, naive and memory T-cell subsets,<br />
and B and NK cells. Surprisingly, counts <strong>of</strong> activated TH and cytotoxic T cells were also higher among LTNPs. LNTPs were<br />
more frequently male. Conclusion: Untreated, HIV-infected Asian children have immune pr<strong>of</strong>iles that differ from those <strong>of</strong><br />
controls, characterized by low values for TH cells, naive T cells, B cells, and NK cells but high values for cytotoxic, activated<br />
TH, and cytotoxic T cells. The higher values for activated T cells observed in LTNPs require confirmation in longitudinal<br />
studies. © <strong>2010</strong> American Academy <strong>of</strong> Allergy, Asthma & Immunology.<br />
No.34<br />
Author (s) : Anderson, T.J.C., Nair, S., Nkhorna, S., Williams, J.T., Imwong, M., Yi, P., Socheat, D., Das, D., Chotivanich, K., Day, N.P.J., White,<br />
N.J., Dondorp, A.M.<br />
Title : High heritability <strong>of</strong> malaria parasite clearance rate indicates a genetic basis for artemisinin resistance in western Cambodia<br />
Source : Journal <strong>of</strong> Infectious Diseases, Volume 201, Issue 9, 1 May <strong>2010</strong>, Pages 1326-1330<br />
Document Type : Article<br />
Keywords : artemisin, antimalarial agent, artemisinin, artemisinin derivative, microsatellite DNA; antibiotic<br />
resistance, article, Cambodia, clinical trial, controlled study, drug efficacy, genome analysis, genotype, human, in vitro<br />
study, major clinical study, malaria, Plasmodium, priority journal, drug effect, drug resistance, genetic<br />
variability, genetics, malaria falciparum, parasitology, Plasmodium falciparum, quantitative trait;<br />
Antimalarials, Artemisinins, Cambodia, Drug Resistance, Genetic Variation, Genotype, Humans, Malaria,<br />
Falciparum, Microsatellite Repeats, Plasmodium falciparum, Quantitative Trait, Heritable<br />
<strong>Abstract</strong> : In western Cambodia, malaria parasites clear slowly from the blood after treatment with artemisinin derivatives, but it is<br />
unclear whether this results from parasite, host, or other factors specific to this population. We measured heritability <strong>of</strong><br />
clearance rate by evaluating patients infected with identical or nonidentical parasite genotypes, using methods analogous to<br />
human twin studies. A substantial proportion (56%-58%) <strong>of</strong> the variation in clearance rate is explained by parasite genetics.<br />
This has 2 important implications: (1) selection with artemisinin derivatives will tend to drive resistance spread and (2)<br />
because heritability is high, the genes underlying parasite clearance rate may be identified by genome-wide association. ©<br />
<strong>2010</strong> by the Infectious Diseases Society <strong>of</strong> America. All rights reserved.<br />
No.35<br />
Author (s) : Anderson, T.J.C., Williams, J.T., Nair, S., Sudimack, D., Barends, M., Jaidee, A., Price, R.N., Nosten, F.<br />
Title : Inferred relatedness and heritability in malaria parasites<br />
Source : Proceedings <strong>of</strong> the Royal Society B: Biological Sciences, Volume 277, Issue 1693, 22 August <strong>2010</strong>, Pages 2531-2540<br />
Document Type : Conference Paper<br />
Keywords : Artemisinin; Clearance rate; Clones; Drug resistance; Heritability; Twins<br />
<strong>Abstract</strong> : Malaria parasites vary in phenotypic traits <strong>of</strong> biomedical or biological interest such as growth rate, virulence, sex ratio and<br />
drug resistance, and there is considerable interest in identifying the genes that underlie this variation. An important first step is<br />
to determine trait heritability (H 2). We evaluate two approaches to measuring H2in natural parasite populations using<br />
relatedness inferred from genetic marker data. We collected single-clone Plasmodium falciparum infections from 185 patients<br />
from the Thailand-Burma border, monitored parasite clearance following treatment with artemisinin combination therapy<br />
(ACT), measured resistance to six antimalarial drugs and genotyped parasites using 335 microsatellites. We found strong<br />
relatedness structure. There were 27 groups <strong>of</strong> two to eight clonally identical (CI) parasites, and 74 per cent <strong>of</strong> parasites<br />
showed significant relatedness to one or more other parasites. Initially, we used matrices <strong>of</strong> allele sharing and variance<br />
components (VC) methods to estimate H2. Inhibitory concentrations (IC2) for six drugs showed significant H 2(0.24 to 0.79, p =<br />
0.06 to 2.85 × 10-9), demonstrating that this study design has adequate power. However, a phenotype <strong>of</strong> current interestparasite<br />
clearance following ACT-showed no detectable heritability (H2= 0-0.09, ns) in this population. The existence <strong>of</strong> CI<br />
parasites allows the use <strong>of</strong> a simple ANOVA approach for quantifying H 2, analogous to that used in human twin studies. This<br />
gave similar results to the VC method and requires considerably less genotyping information. We conclude (i) that H2can be<br />
effectively measured in malaria parasite populations using minimal genotype data, allowing rational design <strong>of</strong> genome-wide<br />
13
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
association studies; and (ii) while drug response (IC50) shows significant H2, parasite clearance following ACT was not heritable<br />
in the population studied. © <strong>2010</strong> The Royal Society.<br />
No.36<br />
Author (s) : Anekboon, K., Phimoltares, S., Lursinsap, C., Tongsima, S., Fucharoen, S.<br />
Title : Searching single nucleotide polymorphism markers to complex diseases using genetic algorithm framework and a BoostMode<br />
support vector machine<br />
Source : <strong>2010</strong> 4th <strong>International</strong> Conference on Bioinformatics and Biomedical Engineering, iCBBE <strong>2010</strong>, <strong>2010</strong>, Article number 5515780<br />
Document Type : Conference Paper<br />
Keywords : Genetic algorithm; Single nucleotide polymorphism; Support vector machine<br />
<strong>Abstract</strong> : With the advent <strong>of</strong> large-scale high density single nucleotide polymorphism (SNP) arrays, case-control association studies have<br />
been performed to identify predisposing genetic factors that influence many common complex diseases. These genotyping<br />
platforms provide very dense SNP coverage per one chip. Much research has been focusing on multivariate genetic model to<br />
identify genes that can predict the disease status. However, increasing the number <strong>of</strong> SNPs generates large number <strong>of</strong><br />
combined genetic outcomes to be tested. This work presents a new mathematical algorithm for SNP analysis called IFGA that<br />
uses a "BoostMode" support vector machine (SVM) to select the best set <strong>of</strong> SNP markers that can predict a state <strong>of</strong> complex<br />
diseases. The proposed algorithm has been applied to test for the association study in two diseases, namely Crohn's and<br />
severity spectrum <strong>of</strong> β0°/Hb E Thalassemia diseases. The results revealed that our predicted SNPs can respectively best<br />
classify both diseases at 71.57% and 71.06% accuracy using 10-fold cross validation comparing with the optimum random<br />
forest (ORF) and classification and regression trees (CART) techniques. © <strong>2010</strong> IEEE.<br />
No.37<br />
Author (s) : Angchaisuksiri P., Worasayan D.<br />
Title : Cancer associated thrombosis: the Thai perspective<br />
Source : THROMBOSIS RESEARCH, Volume: 125, Supplement: 2, Pages: S168-S168, Published: APR <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.38<br />
Author (s) : Angthong, C., Wunnasinthop, S., Sanpakit, S.<br />
Title : Complex lumbosacral fracture-dislocation with pelvic ring disruption and vertical shear sacral fracture: A case report <strong>of</strong> late<br />
presentation and review <strong>of</strong> the literature [Pelvik halka ayrlmas ve vertikal sakral krk ile birlikte kompleks lumbosakral krkdislokasyonu:<br />
Olgunun geç sunumu ve literatürün gözden geçirilmesi]<br />
Source : Ulusal Travma ve Acil Cerrahi Dergisi, Volume 16, Issue 6, November <strong>2010</strong>, Pages 561-566<br />
Document Type : Review<br />
Keywords : Lumbosacral dislocation; Pelvic ring disruption; Spondylo-pelvic injury; Traumatic spondylolisthesis; Vertical shear injury<br />
<strong>Abstract</strong> : Combination <strong>of</strong> lumbosacral junction injury with closed vertical shear sacral fracture and disruption <strong>of</strong> the symphysis pubis is<br />
a very rare pattern <strong>of</strong> injury, particularly with a late presentation. To our knowledge, the complexity <strong>of</strong> such a lumbosacral<br />
injury with pelvic fractures, which was presented with a chronic condition, has never been addressed or identified in the<br />
previous literature. We aimed to demonstrate a case with a late presentation <strong>of</strong> a complex lumbosacral fracture-dislocation,<br />
pelvic ring disruption and a vertical shear sacral fracture with neurological deficits and to emphasize the difficulties in the<br />
management in this case and the operative technique used for the definitive treatment. The initial event had occurred three<br />
months earlier. Closed reduction by skeletal traction had failed; therefore, surgical correction was performed by means <strong>of</strong><br />
indirect reduction via pedicle screws, iliac screws and rods system. Definitive stabilization with posterior lumbo-pelvic<br />
segmental fixation and posterolateral fusion were performed using a posterior approach. At one year after surgery, the clinical<br />
result was satisfactory with almost complete correction <strong>of</strong> a deformity and solid posterolateral fusion. The patient had partial<br />
recovery from the preoperative neurological deficit. We report herein a patient with a very unusual complex spondylopelvic<br />
injury pattern with late presentation, which required meticulous planning <strong>of</strong> management, imaging, and surgical technique<br />
before definitive treatment.<br />
14
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.39<br />
Author (s) : Anh, D.D., Riewpaiboon, A., Tho, L.H., Kim, S.A., Nyambat, B., Kilgore, P.<br />
Title : Treatment costs <strong>of</strong> pneumonia, meningitis, sepsis, and other diseases among hospitalized children in Viet Nam<br />
Source : Journal <strong>of</strong> Health, Population and Nutrition, Volume 28, Issue 5, October <strong>2010</strong>, Pages 436-442<br />
Document Type : Article<br />
Keywords : Cost-<strong>of</strong>-illness; Healthcare costs; Meningitis; Pneumococcal diseases; Pneumonia; Sepsis; Viet nam<br />
<strong>Abstract</strong> : The aim <strong>of</strong> this study was to estimate the costs <strong>of</strong> treatment <strong>of</strong> children who present with the signs and symptoms <strong>of</strong><br />
invasive bacterial diseases in Khanh Hoa province, Viet Nam. The study was an incidencebased cost-<strong>of</strong>-illness analysis from<br />
the health system perspective. The hospital costs included labour, materials and capital costs, both direct and indirect. Costs<br />
were determined for 980 children, with an average age <strong>of</strong> 12.67 months (standard deviation±11.38), who were enrolled in a<br />
prospective surveillance at the Khanh Hoa General Hospital during 2005-2006. Of them, 57% were male. By disease-category,<br />
80% were suspected <strong>of</strong> having pneumonia, 8% meningitis, 3% very severe disease consistent with pneumococcal sepsis, and<br />
9% other diseases. Treatment costs for suspected pneumonia, meningitis, very severe disease, and other diseases were US$<br />
31, US$ 57, US$ 73, and US$ 24 respectively. Costs ranged from US$ 24 to US$ 164 across different case-categories. Both type<br />
<strong>of</strong> disease and age <strong>of</strong> patient had statistically significant effects on treatment costs. The results showed that treatment costs<br />
for bacterial diseases in children were considerable and might differ by as much as seven times among invasive<br />
pneumococcal diseases. Changes in costs were sensitive to both age <strong>of</strong> patient and case-category. These cost-<strong>of</strong>-illness data<br />
will be an important component in the overall evidence base to guide the development <strong>of</strong> vaccine policy in Viet Nam. ©<br />
<strong>International</strong> Centre for Diarrhoeal Disease Research, Bangladesh.<br />
No.40<br />
Author (s) : Antia, B.S., Pansanit, A., Ekpa, O.D., Ekpe, U.J., <strong>Mahidol</strong>, C., Kittakoop, P.<br />
Title : α-Glucosidase inhibitory, aromatase inhibitory, and antiplasmodial activities <strong>of</strong> a biflavonoid gb1 from garcinia kola stem bark<br />
Source : Planta Medica, Volume 76, Issue 3, <strong>2010</strong>, Pages 276-277<br />
Document Type : Article<br />
Keywords : α-glucosidase inhibitor; Antiplasmodial; Aromatase inhibitor; Biflavonoid; Garcinia kola; Guttiferae<br />
<strong>Abstract</strong> : The biflavonoid, 3,4,4,5,5,7,7-heptahydroxy-3,8-biflavanone, known as GB1 (1), was isolated as a major constituent from<br />
Garcinia kola stem bark. GB1 (1) exhibited -glucosidase and aromatase inhibitory activities, as well as antiplasmodial activity,<br />
but was not toxic against cell lines tested. GB1 (1) may be a potential dietary supplement or phytomedicine for the<br />
prevention <strong>of</strong> breast cancer and type 2 diabetes mellitus. © Georg Thieme Verlag KG Stuttgart · New York.<br />
No.41<br />
Author (s) : Anunmana C., Anusavice K.J., Mecholsky John J.Jr.<br />
Title : Interfacial toughness <strong>of</strong> bilayer dental ceramics based on a short-bar, chevron-notch test<br />
Source : DENTAL MATERIALS, Volume: 26, Issue: 2, Pages: 111-117, DOI: 10.1016/j.dental.2009.09.003, Published: FEB <strong>2010</strong><br />
Document Type : Article<br />
Keywords : Chevron-notch; Bilayer; Interfacial toughness; Zirconia; All-ceramic<br />
<strong>Abstract</strong> : Objective. The objective <strong>of</strong> this study was to test the null hypothesis that the interfacial toughness <strong>of</strong> each <strong>of</strong> two types <strong>of</strong><br />
bonded core-veneer bilayer ceramics is not significantly different from the apparent fracture toughness <strong>of</strong> the control<br />
monolithic glass veneer. Methods. T-shaped short-bars <strong>of</strong> a lithia-disilicate glass-ceramic core (LC) and yttria-stabilized<br />
polycrystalline zirconia core ceramic (ZC) were prepared according to the manufacturer's recommendations. V-shaped<br />
notches were prepared by using 25-mu m-thick palladium foil, leaving the chevron-notch area exposed, and the bars were<br />
veneered with a thermally compatible glass veneer (LC/GV and ZC/GV). Additionally, we also bonded the glass veneer to<br />
itself as a control group (GV/GV). Specimens were kept in distilled water for 30 days before testing in tension. Eight glass<br />
veneer bars were prepared for the analysis <strong>of</strong> fracture toughness test using the indentation-strength technique. Results. The<br />
mean interfacial toughness <strong>of</strong> the LC/GV group was 0.69 MPa m(1/2) (0.11), and did not significantly differ from that <strong>of</strong> the<br />
GV/GV control group, 0.74 MP am(1/2) (0.17) (p > 0.05). However, the difference between the mean interfacial toughness <strong>of</strong><br />
the ZC/GV group, 0.13 MPa m(1/2) (0.07), and the LC/GV and the GV/GV groups was statistically significant (p < 0.05).<br />
Significance. For bilayer all-ceramic restorations with high-strength core materials, the veneering ceramics are the weakest link<br />
in the design <strong>of</strong> the structure. Since all-ceramic restorations <strong>of</strong>ten fail from chipping <strong>of</strong> veneer layers or crack initiation at the<br />
interface, the protective effects <strong>of</strong> thermal mismatch stresses oral prosthesis design should be investigated. (C) 2009 Academy<br />
<strong>of</strong> Dental Materials. Published by Elsevier Ltd. All rights reserved.<br />
15
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.42<br />
Author (s) : Anupriwan O., Domrongkitchaiporn S., Tanomsup S., Yamwong S., Sritara P.<br />
Title : Threshold effect <strong>of</strong> urinary sodium excretion on prevalence <strong>of</strong> hypertension in a cohort <strong>of</strong> Thai population<br />
Source : EUROPEAN HEART JOURNAL, Volume: 31, Supplement: 1 ,Pages: 1016-1016, Published: SEP <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.43<br />
Author (s) : Anurukvorakun, O., Buchberger, W., Himmelsbach, M., Klampel, C.W., Suntornsuk, L.<br />
Title : A sensitive non-aqueous capillary electrophoresis-mass spectrometric method for multiresidue analyses <strong>of</strong> β-agonists in pork<br />
Source : Biomedical Chromatography, Volume 24, Issue 6, June <strong>2010</strong>, Pages 588-599<br />
Document Type : Article<br />
Keywords : β-agonists; Clenbuterol; Non-aqueous capillary electrophoresis-mass spectrometry; Salbutamol; Terbutaline<br />
<strong>Abstract</strong> : Non-aqueous capillary electrophoresis-mass spectrometry (NACE-MS) was developed for trace analyses <strong>of</strong> β-agonists (i.e.<br />
clenbuterol, salbutamol and terbutaline) in pork. The NACE was in 18 mM ammonium acetate in methanol-acetonitrile-glacial<br />
acetic acid (66 : 33 : 1, v/v/v) using a voltage <strong>of</strong> 28 kV. The hyphenation <strong>of</strong> CE with a time-<strong>of</strong>-flight MS was performed by<br />
electrospray ionization interface employing 5 mM ammonium acetate in methanol-water (80 : 20, v/v) as the sheath liquid at<br />
a flow rate <strong>of</strong> 2 μL/min. Method sensitivity was enhanced by a co-injection technique (combination <strong>of</strong> hydrodynamic and<br />
electrokinetic injection) using a pressure <strong>of</strong> 50 mbar and a voltage <strong>of</strong> 10 kV for 12 s. The method was validated in comparison<br />
with HPLC-MS-MS. The NACE-MS procedure provided excellent detection limits <strong>of</strong> 0.3 ppb for all analytes. Method linearity<br />
was good (r2 > 0.999, in a range <strong>of</strong> 0.8-1000 ppb for all analytes). Precision showed %RSDs <strong>of</strong> <17.7%. Sample pretreatment<br />
was carried out by solid-phase extraction using mixed mode reversed phase/cation exchange cartridges yielding<br />
recoveries between 69 and 80%. The NACE-MS could be successfully used for the analysis <strong>of</strong> β-agonists in pork samples and<br />
results showed no statistical differences from the values reported by the Ministry <strong>of</strong> Public Health, Thailand using HPLCMS-MS<br />
method. Copyright © 2009 John Wiley & Sons, Ltd.<br />
No.44<br />
Author (s) : Anutarapongpan K., Jongjirasiri S., Laothamatas J., Yamwong S., Sritara P.<br />
Title : Visceral fat as a predictor <strong>of</strong> coronary artery disease as assessed by MSCT in Thai patients<br />
Source : EUROPEAN HEART JOURNAL, Volume: 31, Supplement: 1, Pages: 1003-1003, Published: SEP <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.45<br />
Author (s) : Apipan, B., Rummasak, D.<br />
Title : Efficacy and Safety <strong>of</strong> Oral Propranolol Premedication to Reduce Reflex Tachycardia During Hypotensive Anesthesia With<br />
Sodium Nitroprusside in Orthognathic Surgery: A Double-Blind Randomized Clinical Trial<br />
Source : Journal <strong>of</strong> Oral and Maxill<strong>of</strong>acial Surgery, Volume 68, Issue 1, January <strong>2010</strong>, Pages 120-124<br />
Document Type : Article<br />
Keywords : fentanyl, nitroprusside sodium, nitrous oxide, pancuronium, placebo, prop<strong>of</strong>ol, propranolol, sev<strong>of</strong>lurane, thiopental;<br />
adult, anesthesia induction, article, blood volume, clinical article, clinical trial, controlled clinical trial, controlled<br />
study, double blind procedure, drug dose titration, drug efficacy, drug safety, female, heart arrhythmia, heart<br />
rate, human, hypertension, induced hypotension, male, mean arterial pressure, operation duration, orthognathic<br />
surgery, postoperative hemorrhage, premedication, randomized controlled trial, surgical patient, systolic blood<br />
pressure, tachycardia; Administration, Oral, Adult, Anesthesia, General, Anti-Arrhythmia Agents, Baroreflex, Blood Loss,<br />
Surgical, Double-Blind Method, Female, Heart Rate, Humans, Hypotension, Controlled, Infusions,<br />
Intravenous, Male, Nitroprusside, Orthognathic Surgical Procedures, Premedication, Propranolol, Tachycardia, Vasodilator<br />
Agents<br />
<strong>Abstract</strong> : Purpose: The present study sought to determine whether premedication with oral propranolol 10 mg before hypotensive<br />
anesthesia with sodium nitroprusside could reduce reflex tachycardia, the amount <strong>of</strong> sodium nitroprusside used, and blood<br />
16
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
loss during hypotensive anesthesia for orthognathic surgery. Patients and Methods: A total <strong>of</strong> 60 patients undergoing<br />
bimaxillary surgery were studied in a prospective, randomized, and double-blind study <strong>of</strong> oral propranolol 10 mg or placebo<br />
as premedication before hypotensive anesthesia with sodium nitroprusside. Hemodynamic variables, the amount <strong>of</strong> sodium<br />
nitroprusside used, and blood loss were statistically analyzed. Results: The heart rate and amount <strong>of</strong> sodium nitroprusside<br />
used were highly significantly less (P < .01) in the propranolol group, but no significant difference was found in blood loss<br />
between the 2 groups. No clinically significant complications were observed in either group. Conclusion: Premedication with<br />
oral propranolol 10 mg before hypotensive anesthesia with sodium nitroprusside is safe and effective to reduce reflex<br />
tachycardia and the amount <strong>of</strong> sodium nitroprusside used. © <strong>2010</strong> American Association <strong>of</strong> Oral and Maxill<strong>of</strong>acial Surgeons.<br />
No.46<br />
Author (s) : Apiyasawat S., Ngarmukos T., Sriratanasathavorn C., Kasemsuwan P., Sritara P.<br />
Title : Association <strong>of</strong> statin therapy with incidence <strong>of</strong> ventricular arrhythmia in patients with acute coronary syndrome<br />
Source : EUROPEAN HEART JOURNAL, Volume: 31, Supplement: 1, Pages: 270-270, Published: SEP <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.47<br />
Author (s) : Apiyasawat, S., Prasertwitayakij, N., Ngarmukos, T., Chandanamattha, P., Likittanasombat, K.<br />
Title : Feasibility, efficacy, and safety <strong>of</strong> radi<strong>of</strong>requency catheter ablation for cardiac arrhythmias: A twelve-year experience in<br />
Thailand<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 3, March <strong>2010</strong>, Pages 272-277<br />
Document Type : Review<br />
Keywords : Arrhythmias; Cardiac; Catheter ablation; Tachycardia<br />
<strong>Abstract</strong> : Background: Radi<strong>of</strong>requency catheter ablation (RFCA) is widely accepted as a curative treatment for many types <strong>of</strong> cardiac<br />
arrhythmias. Material and Method: The authors retrospectively reviewed 1,193 cases (mean age 45, 41% male) performed in<br />
the electrophysiology laboratory at Ramathibodi Hospital from 1996 to 2007. Success rate, recurrence rate and complications<br />
were analyzed. Results: Ablations <strong>of</strong> atrioventricular nodal reentrant tachycardia (AVNRT, 428 cases, 35.9%) and<br />
atrioventricular reentrant tachycardia (AVRT, 346 cases, 29%) were the most common procedures. The overall success rate<br />
and recurrence rate <strong>of</strong> RFCA were 88.8% and 6.1% respectively. Complications occurred in 50 patients (4.2%) with 1 death<br />
(0.08%). RFCA <strong>of</strong> AVNRT or AVRT rather than other arrhythmias (Odd ratio 11.46, 95% confidence interval 8.14-16.15, p <<br />
0.001) and adult rather than pediatric patients (Odd ratio for age 1.007, 95% confidence interval 1.003-1.011, p < 0.001) were<br />
independent predictors for successful RFCA. Conclusion: RFCA <strong>of</strong> cardiac arrhythmia in our institution is safe and effective.<br />
No.48<br />
Author (s) : Aramaki, M., Silachamroon, U., Desakorn, V., Maek-A-nantawat, W., Waiwaruwut, J., Jutiwarakun, K., Kim, J.H., Pitisuttithum, P.<br />
Title : Immune reconstitution inflammatory syndrome in adult human immunodeficiency virus-infected patients in Thailand<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 1, January <strong>2010</strong>, Pages 138-145<br />
Document Type : Article<br />
Keywords : HAART; HIV-infected patients; IRIS; Thailand<br />
<strong>Abstract</strong> : Immune reconstitution inflammatory syndrome (IRIS) is an important adverse event among human immunodeficiency virus<br />
(HIV)-infected patients taking highly active antiretroviral therapy (HAART). The epidemiology <strong>of</strong> IRIS in Thailand has not been<br />
well examined, especially among adult HIV-infected patients. In the present study, we reviewed the medical records <strong>of</strong> 174<br />
HIV-infected, antiretroviral therapy-naive patients older than 15 years (the median CD4 count at commencement <strong>of</strong> HAART<br />
was 37 cells/mm3) and compared characteristics <strong>of</strong> patients with and without IRIS. During a 12-month follow-up period after<br />
commencement <strong>of</strong> HAART, 11 cases (6.3%) <strong>of</strong> IRIS were identified (4.2/100 patientyears HAART). The cases included nine<br />
cases with mycobacterial infection, one with cytomegalovirus retinitis and one with cryptococcal meningitis. The patients with<br />
IRIS were significantly younger than those without IRIS (29 vs 36 on medians, p=0.022). The median interval between<br />
commencement <strong>of</strong> HAART and the onset <strong>of</strong> IRIS was 22 days. Although all patients with IRIS improved with or without<br />
corticosteroids, they were more frequently hospitalized during a 12-month follow-up period while taking HAART (1 vs 0 on<br />
medians, p<0.001). The incidence <strong>of</strong> IRIS in advanced adult HIV-infected patients in Thailand was lower than that reported<br />
17
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
from Europe and the United States, which may be attributable to deferment <strong>of</strong> HAART after diagnosing opportunistic<br />
infections.<br />
No.49<br />
Author (s) : Aramwit, P., Muangman, P., Namviriyachote, N., Srichana, T.<br />
Title : In vitro evaluation <strong>of</strong> the antimicrobial effectiveness and moisture binding properties <strong>of</strong> wound dressings<br />
Source : <strong>International</strong> Journal <strong>of</strong> Molecular Sciences, Volume 11, Issue 8, August <strong>2010</strong>, Pages 2864-2874<br />
Document Type : Article<br />
Keywords : Antimicrobial; Moisture absorption; Wound dressing<br />
<strong>Abstract</strong> : A variety <strong>of</strong> silver-coated dressings and some impregnated with other chemicals are now available in the market; however,<br />
there have been few studies analyzing their comparative efficacies as antimicrobial agents. Moreover, their properties for<br />
retaining an appropriate level <strong>of</strong> moisture that is critical for effective wound healing have never been reported. Five<br />
commercially available silver-containing and chlorhexidine dressings, Urgotul SSD®, Bactigras®, Acticoat®, Askina Calgitrol<br />
Ag® and Aquacel Ag®, were tested to determine their comparative antimicrobial effectiveness in vitro against five common<br />
wound pathogens, namely methicillin-sensitive and -resistant Staphylococcus aureus, Bacillus subtilis, Escherichia coli and<br />
Pseudomonas aeruginosa. Mepitel®, a flexible polyamide net coated with s<strong>of</strong>t silicone, was used as a control. The zones <strong>of</strong><br />
inhibition and both the rapidity and the extent <strong>of</strong> killing <strong>of</strong> these pathogens were evaluated. All five antimicrobial dressings<br />
investigated exerted some bactericidal activity, particularly against E. coli. The spectrum and rapidity <strong>of</strong> action ranged widely<br />
for the different dressings. Acticoat® had a broad spectrum <strong>of</strong> action against both Gram-positive and -negative bacteria. Other<br />
dressings demonstrated a narrower range <strong>of</strong> bactericidal activities. Regarding the absorption and release <strong>of</strong> moisture, Askina<br />
Calgitrol Ag® absorbed and released the most moisture from the environment. Aquacel Ag® also exhibited good moisture<br />
absorption and moisture release, but to a lower degree. The other tested dressings absorbed or released very little moisture.<br />
Askina Calgitrol Ag® and Aquacel Ag® are good alternative dressings for treating wounds with high exudates and pus. An<br />
understanding <strong>of</strong> the characteristics <strong>of</strong> these dressings will be useful for utilizing them for specific requirements under<br />
specified conditions. © <strong>2010</strong> by the authors; licensee MDPI, Basel, Switzerland.<br />
No.50<br />
Author (s) : Arayasantiparb, R., Tsuchimochi, M.<br />
Title : Quantification <strong>of</strong> disc displacement in internal derangement <strong>of</strong> the temporomandibular joint using magnetic resonance<br />
imaging<br />
Source : Odontology, Volume 98, Issue 1, February <strong>2010</strong>, Pages 73-81<br />
Document Type : Article<br />
Keywords : Disc; Internal derangement; MRI; Quantification; Temporomandibular joint<br />
<strong>Abstract</strong> : Many measures have been developed to determine the extent <strong>of</strong> disc displacement in internal derangements <strong>of</strong> the<br />
temporomandibular joint (TMJ) using magnetic resonance imaging. The purpose <strong>of</strong> this study was to develop a quantitative<br />
method <strong>of</strong> analyzing disc position and to evaluate the positions <strong>of</strong> the disc in internal derangements <strong>of</strong> the TMJ (group 1,<br />
with reduction; group 2, without reduction). Magnetic resonance images <strong>of</strong> 150 TMJs in 20 healthy volunteers and 55 patients<br />
with internal derangements were evaluated. The anatomical points <strong>of</strong> interest <strong>of</strong> the TMJ, including the anterior (DA) and<br />
posterior (DP) points <strong>of</strong> the disc, were marked on parasagittal magnetic resonance images <strong>of</strong> the TMJ disc taken in both the<br />
closed- and the open-mouth positions. All points were recorded using an x-y coordinate system, with reference to a referral<br />
line. In the closed-mouth position, the DP in patients in group 1 was situated in a more-anterior direction than the DP in<br />
volunteers. The DP in group 2 was located further anterior and inferior than the DP in group 1. However, the position <strong>of</strong> the<br />
DA did not differ between group 1 and group 2. In the open-mouth position, the DP was displaced anteroinferiorly to a<br />
greater extent in group 2 than in group 1 (one-way ANOVA, followed by Scheffe's test; P < 0.0001). The distance between the<br />
disc points in the closed- and open-mouth positions was also evaluated. Comparison <strong>of</strong> the disc point position in the closed-<br />
and open-mouth positions in symptomatic and asymptomatic displaced TMJ discs revealed no significant difference. In<br />
conclusion, most <strong>of</strong> our results quantitatively support previously reported findings in imaging, surgical, and histopathological<br />
studies <strong>of</strong> TMJ internal derangement. We suggest that our measure <strong>of</strong> disc position <strong>of</strong> the TMJ would be useful to assess the<br />
status and response to treatment <strong>of</strong> internal derangements <strong>of</strong> the TMJ. © The Society <strong>of</strong> The Nippon Dental <strong>University</strong> <strong>2010</strong>.<br />
No.51<br />
Author (s) : Arayasiri, M., <strong>Mahidol</strong>, C., Navasumrit, P., Autrup, H., Ruchirawat, M.<br />
18
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Biomonitoring <strong>of</strong> benzene and 1,3-butadiene exposure and early biological effects in traffic policemen<br />
Source : Science <strong>of</strong> the Total Environment, Volume 408, Issue 20, September <strong>2010</strong>, Pages 4855-4862<br />
Document Type : Article<br />
Keywords : 8-Hydroxy-2'-deoxyguanosine (8-OHdG); Benzene and 1,3-butadiene exposure; Biomonitoring; DNA-repair capacity; DNAstrand<br />
breaks; Traffic policemen<br />
<strong>Abstract</strong> : The objective <strong>of</strong> this study was to determine benzene and 1,3-butadiene exposure through ambient air and personal air<br />
monitoring, as well as through biomarkers <strong>of</strong> exposure, and to evaluate the potential health risk <strong>of</strong> exposure through the use<br />
<strong>of</strong> biomarkers <strong>of</strong> early biological effects in central Bangkok traffic policemen. Ambient air concentrations <strong>of</strong> benzene and 1,3butadiene<br />
at the roadsides were significantly higher than in police <strong>of</strong>fices used as control sites (p<0.001). Traffic policemen<br />
had a significantly higher exposure to benzene (median 38.62μg/m3) and 1,3-butadiene (median 3.08μg/m3) than <strong>of</strong>fice<br />
policemen (median 6.17μg/m3 for benzene and 0.37μg/m3 for 1,3-butadiene) (p<0.001). Biomarkers <strong>of</strong> benzene exposure,<br />
blood benzene, and urinary metabolite, trans, trans-muconic acid were significantly higher in traffic policemen than <strong>of</strong>fice<br />
policemen (p<0.001). No significant difference between traffic and <strong>of</strong>fice policemen was found in urinary benzene<br />
metabolite, S-phenyl mercapturic acid, or in urinary 1,3-butadiene metabolite, monohydroxy-butenyl mercapturic acid.<br />
Biomarkers <strong>of</strong> early biological effects, 8-hydroxy-2'-deoxyguanosine in leukocytes (8-OHdG), DNA-strand breaks, and DNA-repair<br />
capacity, measured as an increase in gamma ray-induced chromosome aberrations were significantly higher in traffic<br />
policemen than controls (p<0.001 for 8-OHdG, p<0.01 for tail length, p<0.001 for olive tail moment, p<0.05 for<br />
dicentrics and p<0.01 for deletions). Multiple regression model including individual exposure, biomarkers <strong>of</strong> exposure, ages<br />
and years <strong>of</strong> work as independent variables showed that only the levels <strong>of</strong> individual 1,3-butadiene exposure were<br />
significantly associated with 8-OHdG and olive tail moment at p<0.0001 indicating more influence <strong>of</strong> 1,3-butadiene on DNA<br />
damage. These results indicated that traffic policemen, who are exposed to benzene and 1,3-butadiene at the roadside in<br />
central Bangkok, are potentially at a higher risk for development <strong>of</strong> diseases such as cancer than <strong>of</strong>fice policemen. © <strong>2010</strong><br />
Elsevier B.V.<br />
No.52<br />
Author (s) : Arj-Ong, S., Thakkinstian, A., McEvoy, M., Attia, J.<br />
Title : A systematic review and meta-analysis <strong>of</strong> tumor necrosis factor -308 polymorphism and Kawasaki disease<br />
Source : Pediatrics <strong>International</strong>, Volume 52, Issue 4, August <strong>2010</strong>, Pages 527-532<br />
Document Type : Review<br />
Keywords : genetic association studies; Kawasaki disease; meta-analysis; tumor necrosis factor-alpha-308<br />
<strong>Abstract</strong> : Background: There have been genetic studies assessing the association between tumor necrosis factor (TNF)-α- 308 and<br />
Kawasaki disease (KD) but the results have been conflicting due to lack <strong>of</strong> power. Therefore, a systematic review and metaanalysis<br />
was conducted to increase the power for identifying the association between the TNF-α- 308 polymorphism and KD.<br />
Method: Studies were identified from MEDLINE and EMBASE databases and were included if the subjects were children and<br />
the frequencies between TNF - 308 and KD were reported. Data were pooled using a random effect model if heterogeneity<br />
between studies was present. Results: Thirteen studies were identified however only six studies were included. The pooled<br />
prevalence <strong>of</strong> minor A allele was 5.2% (95% confidence interval [CI]: 0.1%-9.5%). Gene effect was assessed using per-allele<br />
and per-genotype approaches. The pooled odds ratio <strong>of</strong> G versus A with the random effect model was 1.13 (95%CI: 0.34-<br />
3.27). The genotype effects for GG versus GA+AA was estimated and the pooled odds ratio was 1.08 (95%CI: 0.42-2.92).<br />
Conclusion: This review suggests a trend <strong>of</strong> association between the TNF-α-308 G-allele and KD. However, the gene effects<br />
are heterogeneous and assessing sources <strong>of</strong> heterogeneity are limited. An updated meta-analysis is needed if more studies<br />
are published. © <strong>2010</strong> Japan Pediatric Society.<br />
No.53<br />
Author (s) : Arndt, S.S., Lohavech, D., van't Klooster, J., Ohl, F.<br />
Title : Co-species housing in mice and rats: Effects on physiological and behavioral stress responsivity<br />
Source : Hormones and Behavior, Volume 57, Issue 3, March <strong>2010</strong>, Pages 342-351<br />
Document Type : Article<br />
Keywords : Adrenals; Behavior; Co-species housing; Corticosterone; Mouse; Physiology; Rat; Species-separated<br />
housing; Stress; Thymus; Tyrosine hydroxylase<br />
<strong>Abstract</strong> : Co-species housing <strong>of</strong> mice and rats is common practice at most breeding facilities and research laboratories, neglecting the<br />
possible effects on the animals. We investigated physiological as well as behavioral stress-reactivity in mice and rats which<br />
19
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
were either derived from a co-species or species-separated housing condition at the breeding facilities. The animals were kept<br />
under the housing condition they were used to or assigned to the opposite one. Co-species housing had a significant impact<br />
on acute stress reactivity in mice and rats but only if they were used to this housing condition throughout their lives.<br />
Moreover, the stress-effects appeared to be long lasting. Assigning animals, derived from a species-separated housing<br />
condition, to co-species housing led to chronic stress in mice and affected experimental behavior <strong>of</strong> rats. Our findings led to<br />
the conclusion that co-species housing in mice and rats should be avoided, supporting the recommendations by the U.S.<br />
National Institutes <strong>of</strong> Health (NIH) and the Dutch Ministry <strong>of</strong> Health, Welfare and Sport (VWS). In order to support the<br />
interpretation, facilitate the reproducibility and comparability and subsequently the generalizability <strong>of</strong> experimental results,<br />
breeding facilities should at least provide detailed information about their housing conditions. © <strong>2010</strong> Elsevier Inc. All rights<br />
reserved.<br />
No.54<br />
Author (s) : Arphorn, S., Chaonasuan, P., Pruktharathikul, V., Singhakajen, V., Chaikittiporn, C.<br />
Title : A program for thai rubber tappers to improve the cost <strong>of</strong> occupational health and safety<br />
Source : Industrial Health, Volume 48, Issue 3, May <strong>2010</strong>, Pages 275-282<br />
Document Type : Conference Paper<br />
Keywords : Cost <strong>of</strong> occupational health; Health promotion; Rubber tapper; Work-related accident<br />
<strong>Abstract</strong> : The purposes <strong>of</strong> this research were to determine the cost <strong>of</strong> occupational health and safety and work-related health<br />
problems, accidents, injuries and illnesses in rubber tappers by implementing a program in which rubber tappers were<br />
provided training on self-care in order to reduce and prevent work-related accidents, injuries and illnesses. Data on costs for<br />
healthcare, the prevention and the treatment <strong>of</strong> work-related accidents, injuries and illnesses were collected by interview<br />
using a questionnaire. The findings revealed that there was no relationship between what was spent on healthcare and the<br />
prevention <strong>of</strong> work-related accidents, injuries and illnesses and that spent on the treatment <strong>of</strong> work-related accidents, injuries<br />
and illnesses. The proportion <strong>of</strong> the injured subjects after the program implementation was significantly less than that before<br />
the program implementation (p
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Source : Bulletin <strong>of</strong> the World Health Organization, Volume 88, Issue 3, March <strong>2010</strong>, Pages 173-184<br />
Document Type : Article<br />
Keywords : larvicidal agent, virus vector; breeding site, dengue fever, disease control, disease transmission, disease vector, household<br />
survey, periurban area, public health, urban area, waste disposal, water supply; Aedes aegypti, article, Asia, attitude to<br />
health, breeding, city, dengue, Dengue virus, health service, health survey, household, knowledge, larva, pesticide<br />
spraying, pupa (life cycle stage), urban area, vector control, virus transmission, waste disposal, water supply, zoology;<br />
Animals, Asia, Data Collection, Dengue, Disease Reservoirs, Ecosystem, Insect Vectors, Mosquito Control, Suburban<br />
Health, Urban Health; Asia; Aedes aegypti<br />
<strong>Abstract</strong> : Objective: To study dengue vector breeding patterns under a variety <strong>of</strong> conditions in public and private spaces; to explore<br />
the ecological, biological and social (eco-bio-social) factors involved in vector breeding and viral transmission, and to define<br />
the main implications for vector control. Methods: In each <strong>of</strong> six Asian cities or periurban areas, a team randomly selected<br />
urban clusters for conducting standardized household surveys, neighbourhood background surveys and entomological<br />
surveys. They collected information on vector breeding sites, people's knowledge, attitudes and practices surrounding<br />
dengue, and the characteristics <strong>of</strong> the study areas. All premises were inspected; larval indices were used to quantify vector<br />
breeding sites, and pupal counts were used to identify productive water container types and as a proxy measure for adult<br />
vector abundance. Findings: The most productive vector breeding sites were outdoor water containers, particularly if<br />
uncovered, beneath shrubbery and unused for at least one week. Peridomestic and intradomestic areas were much more<br />
important for pupal production than commercial and public spaces other than schools and religious facilities. A complex but<br />
non-significant association was found between water supply and pupal counts, and lack <strong>of</strong> waste disposal services was<br />
associated with higher vector abundance in only one site. Greater knowledge about dengue and its transmission was<br />
associated with lower mosquito breeding and production. Vector control measures (mainly larviciding in one site)<br />
substantially reduced larval and pupal counts and "pushed" mosquito breeding to alternative containers. Conclusion: Vector<br />
breeding and the production <strong>of</strong> adult Aedes aegypti are influenced by a complex interplay <strong>of</strong> factors. Thus, to achieve<br />
effective vector management, a public health response beyond routine larviciding or focal spraying is essential.<br />
No.57<br />
Author (s) : Arunpongpaisal, S., Srisurapanont, M., Kongsakon, R., Kitiwattanagul, K., Samanwongthai, U.<br />
Title : Risperidone long-acting injection (RLAI): The 12-week efficacy and tolerability in thai patients with chronic schizophrenia<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 3, March <strong>2010</strong>, Pages 343-350<br />
Document Type : Article<br />
Keywords : Risperidone long acting injection; Schizophrenia; Thai<br />
<strong>Abstract</strong> : Background: Although oral atypical antipsychotics have improved the outcomes in schizophrenia, the patient medication<br />
adherence plays role as the important factor to clinical potential <strong>of</strong> the drugs. Therefore, the long-acting formulations <strong>of</strong><br />
antipsychotics have been developed to improve the treatment compliance in patient with schizophrenia and risperidone<br />
long-acting injection (RLAI) is the first long-acting injectable drug since then. Objective: To evaluate the efficacy and<br />
tolerability <strong>of</strong> long-acting risperidone injection in Thai patients with chronic schizophrenia for 12 weeks treatment. Material<br />
and Method: This was a non-randomized, open-label, single-arm study, performed at 5 centers in Thailand. The eligible<br />
patients with schizophrenia diagnosed by DSM-IV criteria were enrolled. Patients received long-acting risperidone injection 25,<br />
37.5 or 50 mg every 2 weeks. Efficacy assessments were measured by Manchester Psychiatric Rating Scale (MPS), CGI-S and SF-<br />
36 at baseline, week 6 and week 12 or end point visit. Tolerability assessments were measured by Yale Extrapyramidal<br />
Symptoms Rating Scale (YESS), Visual analogue scale 10-cm for pain at injection site, body weight (BW) and incidence <strong>of</strong><br />
adverse events. Results: Of 184 patients recruited, 160 patients (87%) completed the study. RLAI produced a significant<br />
improvement (p < 0.001) in MPS positive score from baseline to endpoint, 4.4 + 3.7 to 1.6 + 2.6. There was also significant<br />
reduction in MPS negative score, from 3.06 + 2.68 to 0.93 + 1.61 at endpoint (p < 0.001). The CGI-S score improved<br />
significantly from baseline to end point (p < 0.001), as reflected by the increase the proportion <strong>of</strong> patients rated as "not ill" or<br />
"borderline ill" from 5.9% at baseline to 53.2% at endpoint. Quality <strong>of</strong> life measured on the SF-36 scale was improved in all<br />
domains except bodily pain. Movement disorders, measured by YESS, were significantly reduced following RLAI introduction.<br />
Treatment with this drug was well tolerated and no significant weight gain occurred during the study. Conclusion: This study<br />
suggests that RLAI produces symptomatic improvement in chronic schizophrenia patients, along with improvement <strong>of</strong><br />
movement disorders and had a good tolerability and adherence to treatment.<br />
21
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.58<br />
Author (s) : Ashley, E.A., Stepniewska, K., Lindegardh, N., Annerberg, A., Tarning, J., McGready, R., Phaiphun, L., Singhasivanon, P., White,<br />
N.J., Nosten, F.<br />
Title : Comparison <strong>of</strong> plasma, venous and capillary blood levels <strong>of</strong> piperaquine in patients with uncomplicated falciparum malaria<br />
Source : European Journal <strong>of</strong> Clinical Pharmacology, Volume 66, Issue 7, July <strong>2010</strong>, Pages 705-712<br />
Document Type : Article<br />
Keywords : Artemisinin combination therapy; Capillary blood; Malaria; Piperaquine<br />
<strong>Abstract</strong> : Purpose Dihydroartemisinin-piperaquine (DP) is a fixeddose artemisinin-based combination treatment. Field pharmacokinetic<br />
studies would be simplified and facilitated by being able to use small volume capillary assays rather than venous blood. The<br />
aim <strong>of</strong> this study was to describe the relationship between piperaquine concentrations measured in capillary blood, venous<br />
blood and venous plasma. Methods Samples <strong>of</strong> plasma, whole blood obtained by venesection and capillary blood were<br />
taken simultaneously from patients with uncomplicated Plasmodium falciparum malaria treated with DP between 0 and 9<br />
weeks after treatment. Piperaquine concentrations in venous and capillary samples were measured using solid phase<br />
extraction and analysis by liquid chromatography with ultraviolet detection. Results A total <strong>of</strong> 161 sets <strong>of</strong> the three measures<br />
were obtained from 54 patients. Piperaquine concentrations in the venous blood samples were approximately tw<strong>of</strong>old higher<br />
and those in the capillary blood samples were threefold higher than the corresponding venous plasma concentrations.<br />
Capillary blood piperaquine concentrations were approximately 1.7-fold higher than venous blood concentrations, and this<br />
difference also increased with time. Conclusion Differences in whole blood and plasma levels <strong>of</strong> piperaquine suggest<br />
compartmentalisation <strong>of</strong> the drug within blood cells, as also occurs with the structurally related quinoline chloroquine. The<br />
relationship between piperaquine concentrations in the venous plasma, venous blood and capillary blood is variable and<br />
unpredictable at low concentrations. However, within the range <strong>of</strong> concentrations usually present in patients between 3 and<br />
21 days after treatment with currently recommended doses, the relationship between capillary and venous whole blood is<br />
predictable; consequently, capillary blood sampling can be used in field assessments.<br />
No.59<br />
Author (s) : Askie, L.M., Ballard, R.A., Cutter, G., Dani, C., Elbourne, D., Field, D., Hascoet, J.-M., Hibbs, A.M., Kinsella, J.P., Mercier, J.-C., Rich,<br />
W., Schreiber, M.D., Srisuparp, P., Subhedar, N.V., Van Meurs, K.P., Voysey, M., Barrington, K., Ehrenkranz, R.A., Finer, N.<br />
Title : Inhaled Nitric Oxide in preterm infants: A systematic review and individual patient data meta-analysis<br />
Source : BMC Pediatrics, 10, art. no. 15, .<br />
Document Type : Article<br />
Keywords : dexamethasone, lung surfactant, nitric oxide, oxygen; article, assisted ventilation, chronic lung disease, clinical trial, drug dose<br />
reduction, drug efficacy, drug response, drug safety, early intervention, human, meta analysis, oxygen<br />
therapy, prediction, prematurity, respiratory distress syndrome, respiratory failure, systematic review, therapy delay, treatment<br />
outcome, chronic disease, inhalational drug administration, lung disease, mortality, newborn, randomized controlled<br />
trial, regression analysis, review; Administration, Inhalation, Chronic Disease, Humans, Infant, Newborn, Infant,<br />
Premature, Infant, Premature, Diseases, Lung Diseases, Nitric Oxide, Randomized Controlled Trials as Topic, Regression<br />
Analysis, Respiratory Insufficiency, Treatment Outcome<br />
<strong>Abstract</strong> : Background: Preterm infants requiring assisted ventilation are at significant risk <strong>of</strong> both pulmonary and cerebral injury. Inhaled<br />
Nitric Oxide, an effective therapy for pulmonary hypertension and hypoxic respiratory failure in the full term infant, has also<br />
been studied in preterm infants. The most recent Cochrane review <strong>of</strong> preterm infants includes 11 studies and 3,370<br />
participants. The results show a statistically significant reduction in the combined outcome <strong>of</strong> death or chronic lung disease<br />
(CLD) in two studies with routine use <strong>of</strong> iNO in intubated preterm infants. However, uncertainty remains as a larger study<br />
(Kinsella 2006) showed no significant benefit for iNO for this combined outcome. Also, trials that included very ill infants do<br />
not demonstrate significant benefit. One trial <strong>of</strong> iNO treatment at a later postnatal age reported a decrease in the incidence<br />
<strong>of</strong> CLD. The aim <strong>of</strong> this individual patient meta-analysis is to confirm or refute these potentially conflicting results and to<br />
determine the extent to which patient or treatment characteristics may explain the results and/or may predict benefit from<br />
inhaled Nitric Oxide in preterm infants.Methods/Design: The Meta-Analysis <strong>of</strong> Preterm Patients on inhaled Nitric Oxide<br />
(MAPPiNO) Collaboration will perform an individual patient data meta-analysis to answer these important clinical questions.<br />
Studies will be included if preterm infants receiving assisted ventilation are randomized to receive inhaled Nitric Oxide or to a<br />
control group. The individual patient data provided by the Collaborators will be analyzed on an intention-to-treat basis where<br />
possible. Binary outcomes will be analyzed using log-binomial regression models and continuous outcomes will be analyzed<br />
using linear fixed effects models. Adjustments for trial differences will be made by including the trial variable in the model<br />
22
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
specification.Discussion: Thirteen (13) trials, with a total <strong>of</strong> 3567 infants are eligible for inclusion in the MAPPiNO systematic<br />
review. To date 11 trials (n = 3298, 92% <strong>of</strong> available patients) have agreed to participate. Funding was successfully granted<br />
from Ikaria Inc as an unrestricted grant. A collaborative group was formed in 2006 with data collection commencing in 2007. It<br />
is anticipated that data analysis will commence in late 2009 with results being publicly available in <strong>2010</strong>. © <strong>2010</strong> Askie et al;<br />
licensee BioMed Central Ltd.<br />
No.60<br />
Author (s) : Assawamekin, N., Sunetnanta, T., Pluempitiwiriyawej, C.<br />
Title : Ontology-based multiperspective requirements traceability framework<br />
Source : Knowledge and Information Systems, Volume 25, Issue 3, December <strong>2010</strong>, Pages 493-522<br />
Document Type : Article<br />
Keywords : Knowledge management; Multiperspective s<strong>of</strong>tware development; Ontology; Requirements traceability<br />
<strong>Abstract</strong> : Large-scaled s<strong>of</strong>tware development inevitably involves a group <strong>of</strong> stakeholders, each <strong>of</strong> whom may express their<br />
requirements differently in their own terminology and representation depending on their perspectives or perceptions <strong>of</strong> their<br />
shared problems. In view <strong>of</strong> that, the heterogeneity must be well handled and resolved in tracing and managing changes <strong>of</strong><br />
such requirements. This paper presents our multiperspective requirements traceability (MUPRET) framework which deploys<br />
ontology as a knowledge management mechanism to intervene mutual "understanding" without restricting the freedom in<br />
expressing requirements differently. Ontology matching is applied as a reasoning mechanism in automatically generating<br />
traceability relationships. The relationships are identified by deriving semantic analogy <strong>of</strong> ontology concepts representing<br />
requirements elements. The precision and recall <strong>of</strong> traceability relationships generated by the framework are verified by<br />
comparing with a set <strong>of</strong> traceability relationships manually identified by users as a pro<strong>of</strong>-<strong>of</strong>-concept <strong>of</strong> this framework. © 2009<br />
Springer-Verlag London Limited.<br />
No.61<br />
Author (s) : Atchaneeyasakul, L.-O., Trinavarat, A.<br />
Title : Choroidal ruptures after adjuvant intravitreal injection <strong>of</strong> bevacizumab for aggressive posterior retinopathy <strong>of</strong> prematurity<br />
Source : Journal <strong>of</strong> Perinatology, Volume 30, Issue 7, July <strong>2010</strong>, Pages 497-499<br />
Document Type : Article<br />
Keywords : avastin; choroidal rupture; intravitreal bevacizumab; laser photocoagulation; retinopathy <strong>of</strong> prematurity; ROP<br />
<strong>Abstract</strong> : In recent times, laser treatment combined with intravitreal bevacizumab (Avastin) has been used rarely as early investigations<br />
to treat patients with aggressive posterior retinopathy <strong>of</strong> prematurity (ROP). We describe choroidal ruptures that were<br />
documented at 10 weeks after this combined treatment in a preterm infant born at 28 weeks gestation with a birth weight <strong>of</strong><br />
1190 g. Aggressive posterior ROP was diagnosed at the age <strong>of</strong> 6 weeks. Initial treatment included bilateral laser ablation,<br />
followed immediately by intravitreal bevacizumab (IVB, 0.75 mg) in the right eye. On day 5, a remarkable regression <strong>of</strong> plus<br />
sign and fibrovascular proliferation was noted only in the right eye. At this point, additional laser treatment combined with<br />
IVB was given in the left eye, which dramatically resolved plus disease and neovascularization. At 10 weeks after IVB in the<br />
left eye, two sites <strong>of</strong> choroidal ruptures were revealed along the posterior edges <strong>of</strong> laser scars. There was no evidence <strong>of</strong><br />
choroidal neovascularization associated with the choroidal ruptures. Choroidal rupture may occur shortly after laser ablation<br />
plus IVB treatment for aggressive posterior ROP. This occurrence requires recognition to determine whether it is actually<br />
related to the treatment. Long-term follow-up is important for understanding the nature and progression <strong>of</strong> this potentially<br />
serious complication. © <strong>2010</strong> Nature America, Inc. All rights reserved.<br />
No.62<br />
Author (s) : Atchaneeyasakul, L.-O., Trinavarat, A., Pituksung, A., Jinda, W., Thongnoppakhun, W., Limwongse, C.<br />
Title : Mutations in the XLRS1 gene in Thai families with X-linked juvenile retinoschisis<br />
Source : Japanese Journal <strong>of</strong> Ophthalmology, Volume 54, Issue 1, January <strong>2010</strong>, Pages 89-93<br />
Document Type : Article<br />
Keywords : Missense mutation; Retinoschisin; X-linked juvenile retinoschisis; XLRS<br />
<strong>Abstract</strong> : Purpose: To identify genetic mutations <strong>of</strong> the XLRS1 gene and to describe the ocular phenotypes in two unrelated Thai<br />
patients with X-linked juvenile retinoschisis. Methods: Ophthalmic examination, including best-corrected visual acuity and<br />
fundus examination and photography, was performed in all participants. Electroretinography (ERG) and optical coherence<br />
tomography were performed when possible. All six exons <strong>of</strong> the XLRS1 gene were amplified, and mutation screening was<br />
23
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
determined by denaturing high-performance liquid chromatography and DNA sequencing. Results: Two point mutations were<br />
identified, a novel missense mutation c.378A > G (p.D126G) in exon 5 and a reported mutation c.637C > T (p.R213W) in exon<br />
6. The first proband with the p.D126G mutation developed vitreous hemorrhage in both eyes at age 7 months. Foveal and<br />
peripheral schisis with several inner layer holes were detected in both eyes. The second proband with the p.R213W mutation<br />
developed slightly blurred vision at age 10 years. Fundus examination showed numerous fine white dots at the macula<br />
without foveal or peripheral schisis. Electronegative ERG results were documented in both probands. Conclusions: A novel<br />
p.D126G mutation appeared to be associated with a severe phenotype with vitreous hemorrhage developing in infancy. Both<br />
intra- and interfamilial clinical variabilities were recognized in our patients. © <strong>2010</strong> Japanese Ophthalmological Society (JOS).<br />
No.63<br />
Author (s) : Atchaneeyasakul, L.-O., Uiprasertkul, M., Trinavarat, A.<br />
Title : Cyclooxygenase-2 expression in retinoblastoma: An immunohistochemical analysis<br />
Source : Current Eye Research, Volume 35, Issue 3, March <strong>2010</strong>, Pages 242-247<br />
Document Type : Article<br />
Keywords : Angiogenesis; Cyclooxygenase-2; Immunohistochemical analysis; Monoclonal antibody; Retinoblastoma<br />
<strong>Abstract</strong> : Purpose: Increased level <strong>of</strong> cyclooxygenase-2 (COX-2) plays a significant role in the pathogenesis <strong>of</strong> cancers. High expression<br />
<strong>of</strong> COX-2 has been demonstrated in several cancer types including retinoblastoma. However, the in vivo study did not<br />
confirm the anti-proliferative effect <strong>of</strong> COX-2 inhibitor, celecoxib, on a murine transgenic retinoblastoma model. We,<br />
therefore, aim to investigate COX-2 expression in paraffin-embedded retinoblastoma specimens in a larger study group.<br />
Methods: We reviewed 55 retinoblastoma specimens obtained during 1995 to 2005. Clinical and histopathological data were<br />
recorded. Immunohistochemical evaluation <strong>of</strong> COX-2 expression was performed using a rabbit monoclonal antibody to<br />
human cyclooxygenase-2. Results: Forty-four <strong>of</strong> 55 specimens (80%) showed negative immunoreactivity for COX-2 expression.<br />
For the 11 specimens (20%, 95% CI=11.6-32.4%) with positive COX-2, all immunostainings were less than 50% <strong>of</strong> tumor area.<br />
Demographic data and treatment details were available in 53 specimens. Enucleation was performed as a primary treatment<br />
in 43 specimens (81%). Other treatments, mainly systemic chemotherapy, were given prior to enucleation in 10 specimens<br />
(19%). There was no statistical difference in COX-2 expression between the specimens identified as primary and secondary<br />
enucleation (p=0.66). Regarding the histopathological findings, there were no significant differences between COX-2 negative<br />
and COX-2 positive groups. Conclusions: It appears that COX-2 is not overexpressed in our retinoblastoma specimens, which is<br />
different from previous studies. This conflicting data reduces the possibility <strong>of</strong> introducing Cox-2 inhibitors in the treatment <strong>of</strong><br />
retinoblastoma.<br />
No.64<br />
Author (s) : Atichartpongkul, S., Fuangthong, M., Vattanaviboon, P., Mongkolsuk, S.<br />
Title : Analyses <strong>of</strong> the regulatory mechanism and physiological roles <strong>of</strong> Pseudomonas aeruginosa OhrR, a transcription regulator and<br />
a sensor <strong>of</strong> organic hydroperoxides<br />
Source : Journal <strong>of</strong> Bacteriology, Volume 192, Issue 8, April <strong>2010</strong>, Pages 2093-2101<br />
Document Type : Article<br />
Keywords : bacterial protein, hydroperoxide, ohrr protein, tert butyl hydroperoxide, unclassified drug; article, bacterial strain, bacterial<br />
virulence, Caenorhabditis elegans, controlled study, gene expression, gene repression, genetic<br />
transcription, nonhuman, phenotype, physiology, priority journal, protein binding, Pseudomonas aeruginosa, regulatory<br />
mechanism, reverse transcription polymerase chain reaction, site directed mutagenesis; Animals, Bacterial Proteins, Blotting,<br />
Northern, Caenorhabditis elegans, Gene Expression Regulation, Bacterial, Hydrogen Peroxide, Mutagenesis, Site-<br />
Directed, Operon, Promoter Regions, Genetic, Protein Binding, Pseudomonas aeruginosa, Repressor Proteins, Reverse<br />
Transcriptase Polymerase Chain Reaction, tert-Butylhydroperoxide, Virulence; Caenorhabditis elegans, Pseudomonas<br />
aeruginosa<br />
<strong>Abstract</strong> : ohrR encodes an organic hydroperoxide sensor and a transcriptional repressor that regulates organic hydroperoxide-inducible<br />
expression <strong>of</strong> a thiol peroxidase gene, ohr, and itself. OhrR binds directly to the operators and represses transcription <strong>of</strong> these<br />
genes. Exposure to an organic hydroperoxide leads to oxidation <strong>of</strong> OhrR and to subsequent structural changes that result in<br />
the loss <strong>of</strong> the repressor's ability to bind to the operators that allow expression <strong>of</strong> the target genes. Differential induction <strong>of</strong><br />
ohrR and ohr by tert-butyl hydroperoxide suggests that factors such as the repressor's dissociation constants for different<br />
operators and the chemical nature <strong>of</strong> the inducer contribute to OhrR-dependent organic hydroperoxide-inducible gene<br />
expression. ohrR and ohr mutants show increased and decreased resistance to organic hydroproxides, respectively, compared<br />
24
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
to a parental strain. Moreover, the ohrR mutant had a reduced-virulence phenotype in the Pseudomonas aeruginosa-<br />
Caenorhabditis elegans pathogenicity model. Copyright © <strong>2010</strong>, American Society for Microbiology. All Rights Reserved.<br />
No.65<br />
Author (s) : Attasart, P., Kaewkhaw, R., Chimwai, C., Kongphom, U., Namramoon, O., Panyim, S.<br />
Title : Inhibition <strong>of</strong> Penaeus monodon densovirus replication in shrimp by double-stranded RNA<br />
Source : Archives <strong>of</strong> Virology, Volume 155, Issue 6, <strong>2010</strong>, Pages 825-832<br />
Document Type : Article<br />
Keywords : double stranded RNA, virus protein; animal, article, Densovirus, evaluation, gene silencing, genetics, metabolism, Penaeidae,<br />
physiology, Thailand, virology, virus gene, virus replication; Animals, Densovirus, Gene Silencing, Genes, Viral, Penaeidae, RNA,<br />
Double-Stranded, Thailand, Viral Nonstructural Proteins, Viral Structural Proteins, Virus Replication; Decapoda (Crustacea),<br />
Densovirus, Penaeus monodon<br />
<strong>Abstract</strong> : Stunted shrimp caused by Penaeus monodon densovirus (PmDNV) infection is one <strong>of</strong> the main problems leading to a<br />
significant economic loss in Thailand. To control this pandemic disease, a double-stranded-RNA-mediated virus-specific gene<br />
silencing approach was applied to inhibit viral replication. In this study, two dsRNAs corresponding to the non-structural<br />
protein (ns1) and the structural protein (vp) genes <strong>of</strong> PmDNV were synthesized and introduced into shrimp haemolymph prior<br />
to viral challenge. After allowing viral replication for two weeks, the suppression effect by each dsRNA was evaluated by semiquantitative<br />
PCR and compared with the control. A reduction <strong>of</strong> PmDNV in shrimp treated with each dsRNA was observed. In<br />
contrast, a high level <strong>of</strong> viral infection was detected in the control group (NaCl). Based on a limited sample number, we<br />
reached the tentative conclusion that virus-specific dsRNA can inhibit PmDNV replication, in which the dsRNA-ns1was more<br />
effective than the dsRNA-vp. © <strong>2010</strong> Springer-Verlag.<br />
No.66<br />
Author (s) : Attia, J., Thakkinstian, A., McElduff, P., Milne, E., Dawson, S., Scott, R.J., Klerk, N.D., Armstrong, B., Thompson, J.<br />
Title : Detecting genotyping error using measures <strong>of</strong> degree <strong>of</strong> hardy-weinberg disequilibrium<br />
Source : Statistical Applications in Genetics and Molecular Biology, Volume 9, Issue 1, <strong>2010</strong>, Article number 5<br />
Document Type : Article<br />
Keywords : Association study; Disequilibrium coefficient; Genotyping error; Hardy-Weinberg equilibrium<br />
<strong>Abstract</strong> : Tests for Hardy-Weinberg equilibrium (HWE) have been used to detect genotyping error, but those tests have low power<br />
unless the sample size is very large. We assessed the performance <strong>of</strong> measures <strong>of</strong> departure from HWE as an alternative way<br />
<strong>of</strong> screening for genotyping error. Three measures <strong>of</strong> the degree <strong>of</strong> disequilibrium (α, ,D, and F) were tested for their ability to<br />
detect genotyping error <strong>of</strong> 5% or more using simulations and a real dataset <strong>of</strong> 184 children with leukemia genotyped at 28<br />
single nucleotide polymorphisms. The simulations indicate that all three disequilibrium coefficients can usefully detect<br />
genotyping error as judged by the area under the Receiver Operator Characteristic (ROC) curve. Their discriminative ability<br />
increases as the error rate increases, and is greater if the genotyping error is in the direction <strong>of</strong> the minor allele. Optimal<br />
thresholds for detecting genotyping error vary for different allele frequencies and patterns <strong>of</strong> genotyping error but allele<br />
frequency-specific thresholds can be nominated. Applying these thresholds would have picked up about 90% <strong>of</strong> genotyping<br />
errors in our actual dataset. Measures <strong>of</strong> departure from HWE may be useful for detecting genotyping error, but this needs to<br />
be confirmed in other real datasets. © <strong>2010</strong> The Berkeley Electronic Press. All rights reserved.<br />
No.67<br />
Author (s) : Auksornkitti, V., Pongsiri, P., Theamboonlers, A., Rianthavorn, P., Poovorawan, Y., Manujum, K., Luplertlop, N.<br />
Title : Whole-genome characterisation <strong>of</strong> Chikungunya virus from Aedes albopictus collected in Thailand<br />
Source : Annals <strong>of</strong> Tropical Medicine and Parasitology, Volume 104, Issue 3, 1 April <strong>2010</strong>, Pages 265-269<br />
Document Type : Article<br />
Keywords : virus RNA; Aedes albopictus, animal experiment, animal tissue, article, Chikungunya alphavirus, female, genome<br />
analysis, male, nonhuman, nucleotide sequence, phylogenetic tree, Thailand, virus isolation, virus strain; Aedes, Alphavirus<br />
Infections, Animals, Chikungunya virus, Female, Genome, Viral, Insect Vectors, Male, Phylogeny, Reverse Transcriptase<br />
Polymerase Chain Reaction, Thailand; Aedes albopictus, Chikungunya virus<br />
<strong>Abstract</strong> : –<br />
25
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.68<br />
Author (s) : Aung K., Hattasingh W., Limkittikul K., Suntarattiwong P., Chotpitayasunondh T.<br />
Title : CLINICAL DIFFERENCE BETWEEN HOSPITALIZED PAEDIATRIC PANDEMIC H1N1 2009 INFLUENZA AND OTHER ACUTE RESPIRATORY<br />
TRACT INFECTIONS AT QUEEN SIRIKIT NATIONAL INSTITUTE OF CHILD HEALTH, THAILAND<br />
Source : ACTA PAEDIATRICA, Volume: 99, Supplement: 462, Pages: 43-43, Published: DEC <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.69<br />
Author (s) : Aunhachoke K., Bussaratid V., Chirachanakul P., Chua-Intra B., Dhitavat J., Jaisathaporn K., Kaewkungwal J., Kampirapap K.,<br />
Khuhaprema T., Pairayayutakul K., Pitisuttithum P., Sindhvananda J., Thaipisuttikul Y.<br />
Title : HERPES ZOSTER-ASSOCIATED ILLNESSES, QUALITY OF LIFE AND HEALTH-CARE COSTS AMONG 180 THAI PATIENTS<br />
Source : VALUE IN HEALTH, Volume: 13, Issue: 7, Pages: A505-A505, Published: NOV <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.70<br />
Author (s) : Avirutnan, P., Fuchs, A., Hauhart, R.E., Somnuke, P., Youn, S., Diamond, M.S., Atkinson, J.P.<br />
Title : Antagonism <strong>of</strong> the complement component C4 by flavivirus nonstructural protein NS1<br />
Source : Journal <strong>of</strong> Experimental Medicine, Volume 207, Issue 4, 12 April <strong>2010</strong>, Pages 793-806<br />
Document Type : Article<br />
Keywords : classical complement pathway C3 C5 convertase, complement component C1, complement component<br />
C4, lectin, nonstructural protein 1; article, complement activation, controlled study, Dengue virus, enzyme<br />
activity, nonhuman, priority journal, protein cleavage, protein degradation, protein protein interaction, virus cell<br />
interaction, West Nile flavivirus, Yellow fever flavivirus; Animals, Biocatalysis, CHO Cells, Complement C1, Complement C1<br />
Inhibitor Protein, Complement C1s, Complement C3-C5 Convertases, Complement C3b, Complement C4, Complement<br />
C4b, Complement Factor I, Complement Hemolytic Activity Assay, Complement Pathway, Classical, Complement Pathway,<br />
Mannose-Binding Lectin, Cricetinae, Cricetulus, Dengue Virus, Enzyme Precursors, Guinea Pigs, Humans, Kinetics, Neutralization<br />
Tests, Protein Binding, Viral Nonstructural Proteins<br />
<strong>Abstract</strong> : The complement system plays an essential protective role in the initial defense against many microorganisms. Flavivirus NS1<br />
is a secreted nonstructural glycoprotein that accumulates in blood, is displayed on the surface <strong>of</strong> infected cells, and has<br />
been hypothesized to have immune evasion functions. Herein, we demonstrate that dengue virus (DENV), West Nile virus<br />
(WNV), and yellow fever virus (YFV) NS1 attenuate classical and lectin pathway activation by directly interacting with C4.<br />
Binding <strong>of</strong> NS1 to C4 reduced C4b deposition and C3 convertase (C4b2a) activity. Although NS1 bound C4b, it lacked intrinsic<br />
c<strong>of</strong>actor activity to degrade C4b, and did not block C3 convertase formation or accelerate decay <strong>of</strong> the C3 and C5<br />
convertases. Instead, NS1 enhanced C4 cleavage by recruiting and activating the complement-specific protease C1s. By<br />
binding C1s and C4 in a complex, NS1 promotes efficient degradation <strong>of</strong> C4 to C4b. Through this mechanism, NS1 protects<br />
DENV from complement-dependent neutralization in solution. These studies define a novel immune evasion mechanism for<br />
restricting complement control <strong>of</strong> microbial infection. © <strong>2010</strong> Avirutnan et al.<br />
No.71<br />
Author (s) : Avirutnan P., Fuchs A., Hauhart R.E., Somnuke P., Youn S., Diamond M.S., Atkinson J.P.<br />
Title : Inhibition <strong>of</strong> the human complement component C4 by Flavivirus non-structural protein NS1<br />
Source : MOLECULAR IMMUNOLOGY, Volume: 47, Issue: 13, Special Issue: SI, Pages: 2239-2239, DOI: 10.1016/j.molimm.<strong>2010</strong>.05.128,<br />
Meeting <strong>Abstract</strong>: 203, Published: AUG <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
26
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.72<br />
Author (s) : Awab, G.R., Pukrittayakamee, S., Imwong, M., Dondorp, A.M., Woodrow, C.J., Lee, S.J., Day, N.P., Singhasivanon, P., White, N.J.,<br />
Kaker, F.<br />
Title : Dihydroartemisinin-piperaquine versus chloroquine to treat vivax malaria in Afghanistan: An open randomized, non-inferiority,<br />
trial<br />
Source : Malaria Journal, Volume 9, Issue 1, <strong>2010</strong>, Article number 105<br />
Document Type : Article<br />
Keywords : chloroquine, dihydroartemisinin plus piperaquine, DNA, hemoglobin, antimalarial agent, artemisinin<br />
derivative, dihydroquinghaosu, piperaquine, quinoline derivative;<br />
adolescent, adult, Afghanistan, aged, anemia, arm, article, Asia, blood, child, clearance, clinical assessment, clinical<br />
trial, concentration (parameters), controlled clinical trial, DNA extraction, drug efficacy, drug tolerability, endemic<br />
disease, female, fever, film, follow up, headache, human, hypothesis, incidence, infant, infection, jaundice, leukocyte<br />
count, log rank test, major clinical study, malaria, malaria control, male, multivariate analysis, outcome<br />
assessment, parasite, Plasmodium vivax, Plasmodium vivax malaria, polymerase chain reaction, practice<br />
guideline, prophylaxis, pruritus, pulse rate, randomized controlled trial, recurrence risk, recurrent infection, registration, risk<br />
assessment, risk factor, risk reduction, sample size, survival rate, systolic blood pressure, treatment failure, treatment<br />
response, univariate analysis, controlled study, drug administration, drug combination, drug effect, isolation and<br />
purification, parasitology, preschool child, prospective study, recurrent disease, treatment outcome;<br />
Adolescent, Afghanistan, Antimalarials, Artemisinins, Child, Child, Preschool, Chloroquine, Drug Administration Schedule, Drug<br />
Therapy, Combination, Female, Follow-Up Studies, Humans, Infant, Malaria, Vivax, Male, Multivariate Analysis, Plasmodium<br />
vivax, Prospective Studies, Quinolines, Recurrence, Treatment Outcome<br />
<strong>Abstract</strong> : Background: Afghanistan's national guidelines recommend chloroquine for the treatment <strong>of</strong> Plasmodium vivax infection, the<br />
parasite responsible for the majority <strong>of</strong> its malaria burden. Chloroquine resistance in P. vivax is emerging in Asia. Therapeutic<br />
responses across Afghanistan have not been evaluated in detail. Methods. Between July 2007 and February 2009, an openlabel,<br />
randomized controlled trial <strong>of</strong> chloroquine and dihydroartemisinin-piperaquine in patients aged three months and over<br />
with slide-confirmed P. vivax mono-infections was conducted. Consistent with current national guidelines, primaquine was not<br />
administered. Subjects were followed up daily during the acute phase <strong>of</strong> illness (days 0-3) and weekly until day 56. The<br />
primary endpoint was the overall cumulative parasitological failure rate at day 56 after the start <strong>of</strong> treatment, with the<br />
hypothesis being that dihydroartemisinin-piperaquine was non-inferior compared to chloroquine ( = 5% difference in<br />
proportion <strong>of</strong> failures). Results. Of 2,182 individuals with positive blood films for P. vivax, 536 were enrolled in the trial. The<br />
day 28 cure rate was 100% in both treatment groups. Parasite clearance was more rapid with dihydroartemisinin-piperaquine<br />
than chloroquine. At day 56, there were more recurrent infections in the chloroquine arm (8.9%, 95% CI 6.0-13.1%) than the<br />
dihydroartemisinin-piperaquine arm (2.8%, 95% CI 1.4-5.8%), a difference in cumulative recurrence rate <strong>of</strong> 6.1% (2-sided<br />
90%CI +2.6 to +9.7%). The log-rank test comparing the survival curves confirmed the superiority <strong>of</strong> dihydroartemisininpiperaquine<br />
over chloroquine (p = 0.003). Multivariate analysis showed that a lower initial haemoglobin concentration was<br />
also independently associated with recurrence. Both regimens were well tolerated and no serious adverse events were<br />
reported. Conclusions. Chloroquine remains an efficacious treatment for the treatment <strong>of</strong> vivax malaria in Afghanistan. In a<br />
setting where radical therapy cannot be administered, dihydroartemisinin- piperaquine provides additional benefit in terms <strong>of</strong><br />
post-treatment prophylaxis, reducing the incidence <strong>of</strong> recurrence from 4-8 weeks after treatment. Trial Registration. The trial<br />
was registered at ClinicalTrials.gov under identifier NCT00682578. © <strong>2010</strong> Awab et al; licensee BioMed Central Ltd.<br />
No.73<br />
Author (s) : Bae, G., Bae, J., Kim-Lohsoontorn, P., Jeong, J.<br />
Title : Performance <strong>of</strong> SOFC coupled with n-C4H10 autothermal reformer: Carbon deposition and development <strong>of</strong> anode structure<br />
Source : <strong>International</strong> Journal <strong>of</strong> Hydrogen Energy, Volume 35, Issue 22, November <strong>2010</strong>, Pages 12346-12358<br />
Document Type : Article<br />
Keywords : Autothermal reforming; Carbon deposition; Metal supported solid oxide fuel cell; n-C4H10; Solid oxide fuel cell<br />
<strong>Abstract</strong> : The performance deterioration <strong>of</strong> solid oxide fuel cells (SOFCs, Nickel-Yttria stabilized zirconia (Ni-YSZ)/YSZ/lanthanum doped<br />
strontium manganite-YSZ (LSM-YSZ)) coupled with n-C4H10 steam reformers (SR), autothermal reformers (ATR), or catalytic<br />
partial oxidation reformers (CPOX) was examined using an integrated system <strong>of</strong> a micro-reactor reformer and SOFC unit. The<br />
terminal voltage rapidly degraded in CPOX-driven SOFC (oxygen to carbon ratio (OCR) = 0.5) while it was fairly stable for SRdriven<br />
SOFC (steam to carbon ratio (SCR) = 2) over 250 h. For ATR-driven SOFC at near the thermoneutral point (OCR = 0.5<br />
27
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
and steam to carbon ration (SCR) = 1.3), significant deterioration <strong>of</strong> the terminal voltage was observed in 100 h <strong>of</strong> operation.<br />
The main precursors <strong>of</strong> carbon deposition on the SOFC were identified by reformate gas analysis during the tests. In this<br />
study, we reveal that the carbon deposition on the SOFC anode can be affected by not only lower-order hydrocarbons<br />
(C1∼C4), but also by the CO/H2 gas mixture. The change in electrical conductivity <strong>of</strong> the Ni-YSZ cermet used for the SOFC<br />
anode was investigated under different gas mixtures. To investigate the propensity for carbon deposition by each carboncontaining<br />
gas mixture, we defined the ratios <strong>of</strong> steam to specific carbon (C1∼C4 lower-order hydrocarbons and CO) in the<br />
reformate gas (SSCR, steam to specific carbon ratio). To inhibit carbon deposition on SOFC anode, the SSCR must be<br />
sufficiently high. However, the reformer operates near its maximum efficiency at low SSCR value and the higher the SSCR<br />
value, the lower the open circuit voltage and operating power density due to Nernst potential. In this study, a metal-foam<br />
supported SOFC single cell (Ni-YSZ/YSZ/Gd-doped ceria (CGO) buffer layer/lanthanum strontium cobalt ferrite-samarium<br />
doped ceria (LSCF-SDC)), impregnated with catalyst was designed; this novel SOFC was then examined for operation at a low<br />
SSCR value <strong>of</strong> the autothermal reformer conditions (near maximum efficiency <strong>of</strong> n-C4H10 reformer and thermal neutral point,<br />
SSCR = 0.5, OCR = 0.5 and SCR = 1.3). The voltage for the metal-foam supported SOFC impregnated with 0.5 wt% Rh/CGO<br />
remained at a nearly constant value, around 0.8 V, for 200 h under a constant temperature <strong>of</strong> 750 °C and current load <strong>of</strong> 250<br />
mA cm-2. © <strong>2010</strong> Pr<strong>of</strong>essor T. Nejat Veziroglu. Published by Elsevier Ltd. All rights reserved.<br />
No.74<br />
Author (s) : Baimark, Y., Srihanam, P., Srisuwan, Y., Phinyocheep, P.<br />
Title : Preparation <strong>of</strong> porous silk fibroin microparticles by a water-in-oil emulsification-diffusion method<br />
Source : Journal <strong>of</strong> Applied Polymer Science, Volume 118, Issue 2, 15 October <strong>2010</strong>, Pages 1127-1133<br />
Document Type : Article<br />
Keywords : Microparticles; Microspheres; Porous structures; Silk fibroin<br />
<strong>Abstract</strong> : Porous silk fibroin (SF) microparticles were prepared by a simple one-step water-in-oil emulsificationdiffusion method.<br />
Aqueous SF solution and organic phase solvent were used as water and oil phases, respectively. Four organic solvents with<br />
different water solubility were studied, i.e., ethyl acetate, diethyl ether, dichloromethane, and chlor<strong>of</strong>orm. Influences <strong>of</strong><br />
organic phase solvent, water/oil volume ratio, and SF concentration on SF microparticle characteristics were investigated. It<br />
was found that shape <strong>of</strong> the SF microparticles depended upon the type <strong>of</strong> organic phase solvent. The SF microparticles with<br />
hollow or bowl-like shape were fabricated when organic solvents with higher water solubility, which are ethyl acetate and<br />
diethyl ether, were used as the continuous oil phase. While using organic solvents with lower water solubility, i.e.,<br />
dichloromethane and chlor<strong>of</strong>orm resulted in the spherical shape SF microspheres. The water/oil volume ratios and the SF<br />
concentrations did not affect on the bowl-like and spherical shapes <strong>of</strong> SF microparticles. Results from scanning electron<br />
microscope shows that all the SF microparticle matrices were porous structures. Using ethyl acetate and diethyl ether as the<br />
oil phase gave larger SF microparticle sizes than using dichloromethane and chlor<strong>of</strong>orm. The influences <strong>of</strong> water/oil volume<br />
ratio and the SF concentration showed slightly effect on the sizes <strong>of</strong> SF microparticles. © <strong>2010</strong> Wiley Periodicals, Inc.<br />
No.75<br />
Author (s) : Baker, R.L., Tindell, S., Buckley Behan, D., Turpin, P.G., Rosenberger, J.M., Punnakitikashem, P.<br />
Title : Phase I: Creating an electronic prototype to generate equitable hospital nurse-to-patient assignments<br />
Source : CIN - Computers Informatics Nursing, Volume 28, Issue 1, January <strong>2010</strong>, Pages 57-62<br />
Document Type : Article<br />
Keywords : Acuity; Burnout; Nurse specialty; Nurse-to-patient assignment; Patient diagnosis; Prototype; Workload<br />
<strong>Abstract</strong> : This pilot program is a s<strong>of</strong>tware-based prototype providing a nurse-to-patient assignment presented to two groups <strong>of</strong> RNs<br />
enrolled in a nursing research course in a North Texas university. The goal <strong>of</strong> the pilot program was to obtain input regarding<br />
the assessment, functionality, and practicality <strong>of</strong> a nurse-to-patient electronic prototype. Registered nurse students were<br />
given a presurvey, instructions, and details on the use <strong>of</strong> the prototype, followed by a postsurvey. Prototype speed and lack<br />
<strong>of</strong> bias were reported as most favorable. Registered nurse students requested additions <strong>of</strong> multiple diagnoses, patient acuity,<br />
and experience level <strong>of</strong> the nurse to enhance the prototype. Seventy-three percent (n = 24) <strong>of</strong> the participants said that they<br />
would use the prototype, and 15% (n = 5) said that they would not. Copyright © <strong>2010</strong> Lippincott Williams & Wilkins.<br />
No.76<br />
Author (s) : Baldwin, K.; Cervenka, T.; de Souza, M. S.; Paris, R.; Koehler, R.; Thongcharoen, P.; Pitisutitthum, P.; Nitayaphan, S.; Suriyanon,<br />
V.; Robb, M.; Michael, N.; Kim, J.; Kijak, G.<br />
28
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Genetics <strong>of</strong> Fc Gamma Receptors IIa and IIIa in vaccine trial reagents to support the study <strong>of</strong> immune correlates in RV144<br />
Source : AIDS RESEARCH AND HUMAN RETROVIRUSES Volume: 26 Issue: 10 Pages: A71-A71 Published: OCT <strong>2010</strong><br />
Document Type : Meeting Absstract<br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.77<br />
Author (s) : Bamrungsuk, K.; Chompoopong, S.; Tilokskulchai, K.<br />
Title : ELECTRICAL STIMULATION ON THE DENERVATED MUSCLE ATTENUATES NEUROPATHIC PAIN AFTER SPARED NERVE INJURY<br />
Source : JOURNAL OF NEUROCHEMISTRY Volume: 115 Supplement: 1 Pages: 67-68 Published: OCT <strong>2010</strong><br />
Document Type : Meeting Absstract<br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.78<br />
Author (s) : Banhiran, W., Tantilipikorn, P., Metheetrairut, C., Assanasen, P., Bunnag, C.<br />
Title : Quality <strong>of</strong> life in patients with chronic rhinitis after radi<strong>of</strong>requency inferior turbinate reduction<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 8, August <strong>2010</strong>, Pages 950-960<br />
Document Type : Article<br />
Keywords : Chronic rhinitis; Quality <strong>of</strong> life; Radi<strong>of</strong>requency turbinate reduction<br />
<strong>Abstract</strong> : Objective: This prospective, before-and-after study was aimed to demonstrate the effectiveness <strong>of</strong> radi<strong>of</strong>requency (RF) inferior<br />
turbinate reduction in patients with chronic rhinitis refractory to medication on quality <strong>of</strong> life. Material and Method: From<br />
April 2007 to April 2008, there were 28 patients enrolled in the present study. The temperature controlled radi<strong>of</strong>requency<br />
(RF) was applied at inferior turbinate on both sides. To compare before and at the 8th weeks after RF treatment, the patients<br />
were given Rhinoconjuctivitis Quality <strong>of</strong> Life questionnaires (Rcq-36) and questionnaires using visual analog scales (0-10) to<br />
assess subjective symptoms such as the frequency and severity <strong>of</strong> nasal obstruction, nasal discharge, hyposmia, and sneezing.<br />
The acoustic rhinometry and active rhinomanometry were also done to evaluate objective outcomes. Results: At the 8th<br />
week after RF treatment, the patient's quality <strong>of</strong> life in every dimension <strong>of</strong> Rcq-36 and all symptoms revealed a significant<br />
improvement (p < 0.05). Particularly, the severity and frequency <strong>of</strong> nasal congestion were reduced from 6.8 ± 2.5 and 6.3 ±<br />
2.5 to 2.6 ± 2.2 and 2.5 ± 2.1 respectively (p < 0.001). However, there was no statistically significant change in objective<br />
findings. All patients reported only minimal pain and no complication. Conclusion: This is the first study to show that<br />
radi<strong>of</strong>requency inferior turbinate reduction can improve quality <strong>of</strong> life <strong>of</strong> the patients with chronic rhinitis in all dimensions. It<br />
is a simple and minimal invasive procedure, and thus will be a good alternative treatment.<br />
No.79<br />
Author (s) : Banjerdpongchai, R., Kongtawelert, P., Khantamat, O., Srisomsap, C., Chokchaichamnankit, D., Subhasitanont, P., Svasti, J.<br />
Title : Mitochondrial and endoplasmic reticulum stress pathways cooperate in zearalenone-induced apoptosis <strong>of</strong> human leukemic<br />
cells<br />
Source : Journal <strong>of</strong> Hematology and Oncology, Volume 3, <strong>2010</strong>, Article number 50<br />
Document Type : Article<br />
Keywords : apoptosis inducing factor, calreticulin, caspase 3, caspase 8, cell protein, cytochrome c, endoplasmic reticulum protein<br />
29, glucose regulated protein 78, heat shock protein 90, messenger RNA, protein Bax, protein bcl 2, protein bcl xl, reactive<br />
oxygen metabolite, unclassified drug, zearalenone; apoptosis, article, cell cycle arrest, cell cycle G1 phase, cell strain HL<br />
60, cell strain U937, controlled study, cytotoxicity, down regulation, endoplasmic reticulum stress, enzyme activity, flow<br />
cytometry, genetic transcription, human, human cell, leukemia cell, liquid chromatography, mitochondrial membrane<br />
potential, mitochondrion, nitroblue tetrazolium test, nucleotide sequence, peripheral blood mononuclear<br />
cell, polyacrylamide gel electrophoresis, protein expression, protein secretion, reverse transcription polymerase chain<br />
reaction, upregulation, Western blotting, cell survival, drug effect, endoplasmic reticulum, gene expression<br />
pr<strong>of</strong>iling, leukemia, metabolism, pathology, physiological stress, physiology; Apoptosis, Cell Survival, Endoplasmic<br />
Reticulum, Gene Expression Pr<strong>of</strong>iling, HL-60 Cells, Humans, Leukemia, Mitochondria, Reactive Oxygen Species, Stress,<br />
Physiological, U937 Cells, Zearalenone<br />
29
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : Background. Zearalenone (ZEA) is a phytoestrogen from Fusarium species. The aims <strong>of</strong> the study was to identify mode <strong>of</strong><br />
human leukemic cell death induced by ZEA and the mechanisms involved. Methods. Cell cytotoxicity <strong>of</strong> ZEA on human<br />
leukemic HL-60, U937 and peripheral blood mononuclear cells (PBMCs) was performed by using 3-(4,5-dimethyl)-2,5-diphenyl<br />
tetrazolium bromide (MTT) assay. Reactive oxygen species production, cell cycle analysis and mitochondrial transmembrane<br />
potential reduction was determined by employing 2',7'-dichlor<strong>of</strong>luorescein diacetate, propidium iodide and 3,3'-<br />
dihexyloxacarbocyanine iodide and flow cytometry, respectively. Caspase-3 and -8 activities were detected by using<br />
fluorogenic Asp-Glu-Val-Asp-7-amino-4- methylcoumarin (DEVD-AMC) and Ile-Glu-Thr-Asp-7-amino-4-methylcoumarin (IETD-<br />
AMC) substrates, respectively. Protein expression <strong>of</strong> cytochrome c, Bax, Bcl-2 and Bcl-xL was performed by Western blot. The<br />
expression <strong>of</strong> proteins was assessed by two-dimensional polyacrylamide gel-electrophoresis (PAGE) coupled with LC-MS2<br />
analysis and real-time reverse transcription polymerase chain reaction (RT-PCR) approach. Results. ZEA was cytotoxic to U937<br />
> HL-60 > PBMCs and caused subdiploid peaks and G1 arrest in both cell lines. Apoptosis <strong>of</strong> human leukemic HL-60 and U937<br />
cell apoptosis induced by ZEA was via an activation <strong>of</strong> mitochondrial release <strong>of</strong> cytochrome c through mitochondrial<br />
transmembrane potential reduction, activation <strong>of</strong> caspase-3 and -8, production <strong>of</strong> reactive oxygen species and induction <strong>of</strong><br />
endoplasmic reticulum stress. Bax was up regulated in a time-dependent manner and there was down regulation <strong>of</strong> Bcl-xL<br />
expression. Two-dimensional PAGE coupled with LC-MS2 analysis showed that ZEA treatment <strong>of</strong> HL-60 cells produced<br />
differences in the levels <strong>of</strong> 22 membrane proteins such as apoptosis inducing factor and the ER stress proteins including<br />
endoplasmic reticulum protein 29 (ERp29), 78 kDa glucose-regulated protein, heat shock protein 90 and calreticulin, whereas<br />
only ERp29 mRNA transcript increased. Conclusion. ZEA induced human leukemic cell apoptosis via endoplasmic stress and<br />
mitochondrial pathway. © <strong>2010</strong> Banjerdpongchai et al; licensee BioMed Central Ltd.<br />
No.80<br />
Author (s) : Banklaui, C., Jindadamrongwech, S., Sawangpanich, R., Apibal, S., Hongeng, S., Paisooksantivatana, K., Pakakasama, S.<br />
Title : Effect <strong>of</strong> genetic alterations <strong>of</strong> cytarabine-metabolizing enzymes in childhood acute lymphoblastic leukemia<br />
Source : Hematology/ Oncology and Stem Cell Therapy, Volume 3, Issue 3, <strong>2010</strong>, Pages 103-108<br />
Document Type : Article<br />
Keywords : cytarabine, cytidine deaminase, deoxycytidine kinase, drug metabolizing enzyme; acute lymphoblastic<br />
leukemia, adolescent, allele, anemia, article, cancer staging, childhood leukemia, clinical protocol, diarrhea, drug<br />
exposure, drug megadose, drug metabolism, drug response, enzyme activity, febrile neutropenia, female, flow<br />
cytometry, genetic variability, genotype, human, infant, leukemia remission, low drug dose, major clinical<br />
study, male, minimal residual disease, mucosa inflammation, mutation, neutropenia, repeated drug dose, risk<br />
assessment, single nucleotide polymorphism, thrombocytopenia; Adolescent, Alleles, Antigens, CD19, Antigens,<br />
CD45, Antimetabolites, Antineoplastic, Child, Child, Preschool, Cytarabine, Cytidine Deaminase, Deoxycytidine Kinase, Dose-<br />
Response Relationship, Drug, Female, Flow Cytometry, Gene<br />
Frequency, Genotype, Humans, Infant, Male, Mucositis, Neoplasm Staging, Neoplasm, Residual, Polymorphism, Single<br />
Nucleotide, Precursor Cell Lymphoblastic Leukemia-Lymphoma, Thrombocytopenia, Treatment Outcome<br />
<strong>Abstract</strong> : BACKGROUND: Single nucleotide polymorphisms (SNPs) <strong>of</strong> deoxycytidine kinase (dCK) and cytidine deaminase (CDA) are<br />
known to alter their enzymatic activities, which affect the metabolism <strong>of</strong> cytarabine. Currently, treatment <strong>of</strong> childhood acute<br />
lymphoblastic leukemia (ALL) includes cytarabine, especially in high-risk patients. Therefore, we hypothesized that a genetic<br />
variation <strong>of</strong> dCK and CDA genes may influence the risk <strong>of</strong> cytarabine-related toxicities and early response to treatment.<br />
PATIENTS AND METHODS: We included children diagnosed with ALL and lymphoblastic lymphoma (LL) stage III and IV. The<br />
patients received a modified St Jude Total Therapy Study XV protocol. Cytarabine was used during induction remission (lowdose<br />
cytarabine) and reinduction II (high-dose cytarabine) phases. Genotyping <strong>of</strong> dCK -360C>G and -201C>T and CDA 79A>C<br />
and 208G>A was performed. Minimal residual disease (MRD) at the end <strong>of</strong> the induction phase was measured using flow<br />
cytometry. RESULTS: Ninety-four children with ALL (n=90) and LL (n=4) were analyzed. The median age at diagnosis was 5.8<br />
years (range, 0.4-15 years). All four SNPs showed predominant wild type alleles. There was no CDA-208A allele in our<br />
population. Children with dCK-360G allele were at risk <strong>of</strong> mucositis after receiving low-dose cytarabine (OR=3.7; 95%CI, 1.2--<br />
11.3). Neither dCK nor CDA polymorphisms affected the MRD status at the end <strong>of</strong> induction phase. CONCLUSION: The dCK-<br />
360G allele was found to increase the risk <strong>of</strong> mucositis after exposure to low-dose cytarabine in childhood ALL therapy.<br />
No.81<br />
Author (s) : Banyai, W., Kirdmanee, C., Mii, M., Supaibulwatana, K.<br />
Title : Overexpression <strong>of</strong> farnesyl pyrophosphate synthase (FPS) gene affected Artemisinin content and growth <strong>of</strong> Artemisia annua L<br />
30
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Source : Plant Cell, Tissue and Organ Culture, Volume 103, Issue 2, November <strong>2010</strong>, Pages 255-265<br />
Document Type : Article<br />
Keywords : Agrobacterium tumefaciens; Artemisia annua L.; Farnesyl pyrophosphate synthase; Transformation; Vacuum infiltration<br />
<strong>Abstract</strong> : Transgenic plants <strong>of</strong> Artemisia annua L., a medicinal plant that produces the compound artemisinin which has an anti-malarial<br />
activity, were developed following Agrobacterium tumefaciens-mediated transformation <strong>of</strong> leaf explants. A. tumefaciens strain<br />
EHA105 carrying either pCAMBIA1301 or pCAMBIAFPS was used. Both plasmids harbored the hygromycin phosphotransferase II<br />
(hptII) gene as a selectable gene, but the latter plasmid also harbored the gene encoding for farnesyl pyrophosphate<br />
synthase (FPS), a key enzyme for artemisinin biosynthesis. Shoot regeneration was observed either directly from leaf sections<br />
or via intervening callus when explants were incubated on solidified Murashige and Skoog (MS) (1962) medium containing 0.1<br />
mg l-1 α-naphthaleneacetic acid (NAA), 1 mg l-1 N6-benzyladenine (BA), 30 mg l-1 meropenem and 10 mg l-1 hygromycin.<br />
Applying vacuum infiltration dramatically increased transformation efficiency up to 7.3 and 19.7% when plasmids with and<br />
without FPS gene were used, respectively. All putative transgenic regenerants showed positive bands <strong>of</strong> hptII gene following<br />
Southern blot analysis. Expression <strong>of</strong> FPS was observed in all transgenic lines, and FPS over-expressed lines exhibited higher<br />
artemisinin content and yield, <strong>of</strong> 2.5- and 3.6-fold, respectively, than that detected in wild-type plants. A relatively high<br />
correlation (R2 = 0.78) was observed between level <strong>of</strong> expression <strong>of</strong> FPS and artemisinin content. However, gene silencing<br />
was detected in some transgenic lines, especially for those lines containing two copies <strong>of</strong> the FPS transgene, and with some<br />
lines exhibiting reduced growth. © <strong>2010</strong> Springer Science+Business Media B.V.<br />
No.82<br />
Author (s) : Banyai, W., Sangthong, R., Karaket, N., Inthima, P., Mii, M., Supaibulwatana, K.<br />
Title : Overproduction <strong>of</strong> artemisinin in tetraploid Artemisia annua L<br />
Source : Plant Biotechnology, Volume 27, Issue 5, <strong>2010</strong>, Pages 427-433<br />
Document Type : Article<br />
Keywords : Artemisia annua L.; Artemisinin; Chromosome; Colchicines; Tetraploid<br />
<strong>Abstract</strong> : Tetraploid plants <strong>of</strong> Artemisia annua L. exhibiting high-artemisinin-yield were successfully induced by treating excised leaves<br />
<strong>of</strong> in vitro plant with 0.1% colchicine. The chromosome number <strong>of</strong> original diploid plant was confirmed to be 2n=2x=18<br />
whereas that <strong>of</strong> the tetraploid plant was 2n=x=6. Morphological and anatomical characteristics <strong>of</strong> tetraploid plants were<br />
obviously different from the diploid counterpart. The tetraploid plants had larger sizes <strong>of</strong> root system, stomata and glandular<br />
secretory trichomes than diploid plant, whereas leaf size in tetraploid was smaller but thicker than diploid. The highest<br />
amount <strong>of</strong> artemisinin content was produced at flower blooming stage in both diploid (2.4% dry weight) and tetraploid (3.8%<br />
dry weight) lines. Based on the maximum yield <strong>of</strong> artemisinin, the optimum harvest time for the diploid lines was flower<br />
initiation stage but it was at full blooming stage for tetraploid lines. The highest artemisinin yield in tetraploid plants up to 3.0<br />
mg plant-1 was detected at this stage which was 1.5 times greater than diploid plants. This is a first report that clearly<br />
showed the potential <strong>of</strong> chromosome doubling strategy to produce high-yield line <strong>of</strong> A. annua plant for artemisinin<br />
production. © <strong>2010</strong> The Japanese Society for Plant Cell and Molecular Biology.<br />
No.83<br />
Author (s) : Baowan, D., Cox, B.J., Hill, J.M.<br />
Title : Discrete and continuous approximations for nanobuds<br />
Source : Fullerenes Nanotubes and Carbon Nanostructures, Volume 18, Issue 2, March <strong>2010</strong>, Pages 160-177<br />
Document Type : Article<br />
Keywords : Calculus <strong>of</strong> variations; Carbon nanotubes; Fullerenes; Least squares method; Nanobuds<br />
<strong>Abstract</strong> : Both discrete and continuous approximations are employed to determine the join structure involved in the novel carbon<br />
nanostructure formed from a C 60 fullerene and a carbon nanotube, namely a nanobud. First, following the methodology <strong>of</strong><br />
the authors' previous work, using a least squares minimization procedure, discrete models are used to join a fullerene and a<br />
carbon nanotube by minimizing both the variation in the bond length and the variation in the bond angle. These purely<br />
geometrical approaches are closely related to the bonded potential energy method adopted by several authors. Second, by<br />
employing a calculus <strong>of</strong> variations technique, a continuous approximation to the nanobud is determined. In this case, there<br />
are also two models depending upon the sign <strong>of</strong> the curvature <strong>of</strong> the join pr<strong>of</strong>ile. We find that the discrete and the<br />
continuous approximations are in reasonable overall agreement. However, there is no experimental or simulation data to<br />
determine which procedure gives the more realistic results. Copyright © Taylor & Francis Group, LLC.<br />
31
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.84<br />
Author (s) : Baowan, D., Cox, B.J., Hill, J.M.<br />
Title : Dislodgement <strong>of</strong> carbon nanotube bundles under pressure driven flow<br />
Source : Nanotechnology, Volume 21, Issue 15, <strong>2010</strong>, Article number 155305<br />
Document Type : Article<br />
Keywords : A-carbon, Carbon nanotube bundle, Cross sectional area, Flow area, Mass transport, Mathematical<br />
modelling, matrix, Nanotube bundles, Poiseuille flow, Pressure force, Pressure-driven flows, Static pressure, Total<br />
force, Viscous forces; Transport properties; Carbon nanotubes<br />
<strong>Abstract</strong> : Experimental and predicted flow rates through carbon nanotubes vary considerably but generally are reported to be well in<br />
excess <strong>of</strong> that predicted by the conventional Poiseuille flow, and therefore nanotubes embedded in a matrix might provide<br />
membranes with exceptional mass transport properties. In this paper, applied mathematical modelling is undertaken to<br />
estimate the three forces acting on a nanotube bundle, namely the molecular interaction force, the viscous force, and the<br />
static pressure force. In deducing estimates <strong>of</strong> these forces we introduce a modification <strong>of</strong> the notion <strong>of</strong> the effective dead<br />
area for a carbon nanotube membrane, and we calculate the total forces necessary to push one or more <strong>of</strong> the nanotubes<br />
out <strong>of</strong> the bundle, thus creating a channel through which further enhancement <strong>of</strong> flow may take place. However, careful<br />
analysis shows that the nett dislodgement force is entirely independent on the useable flow area, but rather depends only<br />
on the total cross-sectional area perpendicular to the flow. This rather surprising result is a consequence <strong>of</strong> the flow being<br />
steady and a balance <strong>of</strong> the viscous and pressure forces. © <strong>2010</strong> IOP Publishing Ltd.<br />
No.85<br />
Author (s) : Baowan, D., Traimpo, W., Traimpo, D., Hill, J.M.<br />
Title : Theoretical prediction for the encapsulation <strong>of</strong> TiO2 nanoparticles into carbon nanotubes<br />
Source : ICONN <strong>2010</strong> - Proceedings <strong>of</strong> the <strong>2010</strong> <strong>International</strong> Conference on Nanoscience and Nanotechnology, <strong>2010</strong>, Article number<br />
6045177, Pages 62-65<br />
Document Type : Conference Paper<br />
Keywords : Carbon nanotube; Encapsulation; Lennard-Jones potential function; Suction energy; Titanium dioxide<br />
<strong>Abstract</strong> : Carbon nanotubes and titanium dioxide nanoparticles (TiO2) are well-known nanostructures which <strong>of</strong>fer the means to create<br />
many new and novel nanoscale devices. The encapsulation <strong>of</strong> a TiO2 molecule into a single-walled carbon nanotube may<br />
provide the ideas for the development in targeted drug delivery process. This paper aims to investigate the relation between<br />
the potential molecular interaction energy <strong>of</strong> the system versus the radii <strong>of</strong> TiO2 molecule and the nanotube. The Lennard-<br />
Jones potential function together with the continuous approximation is employed to determine the molecular interaction<br />
energy between the TiO2 molecule and the carbon nanotube. The suction energy, which is the energy acquired by the<br />
molecule in the encapsulation, for a particular TiO2 molecular radius is presented. An accumulation <strong>of</strong> TiO2 clusters at only<br />
one end <strong>of</strong> the open nanotube is observed in experiments, so the interaction energy between two TiO2 nanoparticles is also<br />
examined. The results presented here are based on work by the present authors appearing in [1, 2]. © <strong>2010</strong> IEEE.<br />
No.86<br />
Author (s) : Baowan, D., Triampo, D., Triampo, W.<br />
Title : Modeling <strong>of</strong> titania nanoparticle accumulation at the open end <strong>of</strong> single-walled carbon nanotubes prior to TiO2<br />
encapsulation<br />
Source : Journal <strong>of</strong> Computational and Theoretical Nanoscience, Volume 7, Issue 10, October <strong>2010</strong>, Pages 1926-1930<br />
Document Type : Article<br />
Keywords : Carbon nanotubes; Lennard-Jones potential; Titanium dioxide<br />
<strong>Abstract</strong> : Carbon nanotubes (CNTs) and titanium dioxide (TiO2) are perhaps the most well-known nanoparticles. The encapsulation <strong>of</strong><br />
TiO2 into CNT could provide ideas for the development <strong>of</strong> targeted drug delivery systems. It is intuitively understood that<br />
due to particle size limitation, the relatively large size <strong>of</strong> TiO2 prevents its encapsulation into CNT. However, it could be<br />
possible for TiO2 to encapsulate when those two types <strong>of</strong> particles are still <strong>of</strong> comparable size. Motivated by experimental<br />
results, the aim <strong>of</strong> this paper is to utilize the Lennard-Jones (6-12) potential function and applied mathematical modeling<br />
tools to understand why TiO2 nanoparticles aggregate at only one side <strong>of</strong> the carbon nanotube in order to be encapsulated<br />
into the tube (which is part <strong>of</strong> the main problem surrounding the encapsulation <strong>of</strong> TiO2 nanoparticles into SWNT). The<br />
theoretical prediction suggests that TiO2 particles are likely to cluster together since the energy level between two TiO2<br />
molecules and a carbon nanotube is lower than that between a TiO2 molecule and a carbon nanotube. This work could be<br />
32
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
considered as one <strong>of</strong> the first step models to be used with the analytic approach before developing more complicate ones.<br />
Copyright © <strong>2010</strong> American Scientific Publishers All rights reserved.<br />
No.87<br />
Author (s) : Barbazan, P., Guiserix, M., Boonyuan, W., Tuntaprasart, W., Pontier, D., Gonzalez, J.-P.<br />
Title : Modelling the effect <strong>of</strong> temperature on transmission <strong>of</strong> dengue<br />
Source : Medical and Veterinary Entomology, Volume 24, Issue 1, March <strong>2010</strong>, Pages 66-73<br />
Document Type : Article<br />
Keywords : Bloodmeals; Daily survival rate; Dengue transmission; Extrinsic incubation period; Gonotrophic cycle; Infective<br />
bites; Model; Temperature; Vector<br />
<strong>Abstract</strong> : The main entomological parameters involved in the rate <strong>of</strong> dengue virus transmission include the longevity <strong>of</strong> female<br />
mosquitoes, the time interval between bites and the extrinsic incubation period <strong>of</strong> the virus. Field and laboratory data<br />
provide estimates for these parameters, but their interactions with other factors (e.g. host population density and<br />
environmental parameters) make their integration into a transmission model quite complex. To estimate the impact <strong>of</strong> these<br />
parameters on transmission, we developed a model <strong>of</strong> virus transmission by a vector population which predicts the number<br />
<strong>of</strong> potentially infective bites under a range <strong>of</strong> temperatures and entomological parameters, including the daily survival rate <strong>of</strong><br />
females, the interval between bites and the extrinsic incubation period. Results show that in a stable population, an increase<br />
in mosquito longevity disproportionately enhances the number <strong>of</strong> potential transmissions (e.g. by as much as five times when<br />
the survival rate rises from 0.80 to 0.95). Halving the length <strong>of</strong> the biting interval with a 10-°C rise in temperature increases the<br />
transmission rate by at least 2.4 times. Accordingly, the model can predict changes in dengue transmission associated with<br />
short-term variation in seasonal temperature and also with potentially long-lasting increases in global temperatures. © <strong>2010</strong><br />
Journal compilation © <strong>2010</strong> The Royal Entomological Society. No claim to original US government works.<br />
No.88<br />
Author (s) : Barzi, F., Woodward, M., Czernichow, S., Lee, C.M.Y., Kang, J.H., Janus, E., Lear, S., Patel, A., Caterson, I., Patel, J., Lam, T.H.,<br />
Suriyawongpaisal, P., Huxley, R.<br />
Title : The discrimination <strong>of</strong> dyslipidaemia using anthropometric measures in ethnically diverse populations <strong>of</strong> the Asia-pacific region:<br />
The obesity in Asia collaboration<br />
Source : Obesity Reviews, Volume 11, Issue 2, February <strong>2010</strong>, Pages 127-136<br />
Document Type : Article<br />
Keywords : Diagnosis; Lipids; Meta-analysis; Obesity<br />
<strong>Abstract</strong> : Dyslipidaemia is a major risk factor for cardiovascular disease and is only detectable through blood testing, which may not be<br />
feasible in resource-poor settings. As dyslipidaemia is commonly associated with excess weight, it may be possible to identify<br />
individuals with adverse lipid pr<strong>of</strong>iles using simple anthropometric measures. A total <strong>of</strong> 222 975 individuals from 18 studies<br />
were included as part <strong>of</strong> the Obesity in Asia Collaboration. Linear and logistic regression models were used to assess the<br />
association between measures <strong>of</strong> body size and dyslipidaemia. Body mass index, waist circumference, waist: hip ratio (WHR)<br />
and waist: height ratio were continuously associated with the lipid variables studied, but the relationships were consistently<br />
stronger for triglycerides and high-density lipoprotein cholesterol. The associations were similar between Asians and non-<br />
Asians, and no single anthropometric measure was superior at discriminating those individuals at increased risk <strong>of</strong><br />
dyslipidaemia. WHR cut-points <strong>of</strong> 0.8 in women and 0.9 in men were applicable across both Asians and non-Asians for the<br />
discrimination <strong>of</strong> individuals with any form <strong>of</strong> dyslipidaemia. Measurement <strong>of</strong> central obesity may help to identify those<br />
individuals at increased risk <strong>of</strong> dyslipidaemia. WHR cut-points <strong>of</strong> 0.8 for women and 0.9 for men are optimal for discriminating<br />
those individuals likely to have adverse lipid pr<strong>of</strong>iles and in need <strong>of</strong> further clinical assessment. © 2009 <strong>International</strong><br />
Association for the Study <strong>of</strong> Obesity.<br />
No.89<br />
Author (s) : Bassat, Q., Allabi, A., Cousin, M., Krudsood, S.<br />
Title : EFFICACY OF ARTEMETHER-LUMEFANTRINE (AL) IN THE TREATMENT OF BLOOD STAGES OF PLASMODIUM VIVAX<br />
Source : AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, Volume: 83, Issue: 5, Supplement: S, Pages: 109-110, Meeting<br />
<strong>Abstract</strong>: 366, Published: NOV <strong>2010</strong><br />
Document Type : Meeting Absstract<br />
Keywords : –<br />
33
<strong>Abstract</strong> : –<br />
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.90<br />
Author (s) : Batra, R., Cooper, B.S., Whiteley, C., Patel, A.K., Wyncoll, D., Edgeworth, J.D.<br />
Title : Efficacy and limitation <strong>of</strong> a Chlorhexidine-Based decolonization strategy in preventing transmission <strong>of</strong> Methidllin-Resistant<br />
staphylococcus aureus in an intensive care unit<br />
Source : Clinical Infectious Diseases, Volume 50, Issue 2, 15 January <strong>2010</strong>, Pages 210-217<br />
Document Type : Article<br />
Keywords : bacterial protein, chlorhexidine acetate, chlorhexidine gluconate, cx antiseptic dusting powder, qacA protein, qacB<br />
protein, triclosan, unclassified drug; adult, aged, antibiotic resistance, antibiotic sensitivity, article, bacterial gene, bacterial<br />
strain, bacterial transmission, bacterium isolate, clinical protocol, controlled study, disinfection, female, hospital bed<br />
capacity, human, in vitro study, in vivo study, infection control, infection prevention, intensive care unit, major clinical<br />
study, male, methicillin resistant Staphylococcus aureus, methicillin resistant Staphylococcus aureus infection, minimum<br />
inhibitory concentration, nonhuman, patient education, priority journal;<br />
Chlorhexidine, Disinfectants, Female, Humans, Intensive Care Units, Male, Methicillin-Resistant Staphylococcus aureus, Middle<br />
Aged, Retrospective Studies, Staphylococcal Infections<br />
<strong>Abstract</strong> : Background. Surface-active antiseptics, such as Chlorhexidine, are increasingly being used as part <strong>of</strong> intervention programs to<br />
prevent methicillin-resistant Staphylococcus aureus (MRSA) transmission, despite limited evidence and potential for<br />
resistance. We report on the effect <strong>of</strong> an antiseptic protocol on acquisition <strong>of</strong> both endemic MRSA and an outbreak strain <strong>of</strong><br />
MRSA sequence type 239 (designated TW). Methods. Interrupted time-series data on MRSA acquisitions in two 15-bed<br />
intensive care units were analyzed using segmented regression models to estimate the effects <strong>of</strong> sequential introduction <strong>of</strong><br />
an educational campaign, cohorting, and a chlorhexidine-based antiseptic protocol on transmission <strong>of</strong> TW and non-TW MRSA<br />
strains. Representative TW and non-TW MRSA strains were assessed for carriage <strong>of</strong> qacA/B genes and antiseptic susceptibility.<br />
Results. The antiseptic protocol was associated with a highly significant, immediate 70% reduction in acquisition <strong>of</strong> non-TW<br />
MRSA strains (estimated model-averaged incidence rate ratio, 0.3; 95% confidence interval, 0.19-0.47) and an increase in<br />
acquisition <strong>of</strong> TW MRSA strains (estimated model-averaged incidence rate ratio, 3.85; 95% confidence interval, 0.80-18.59).<br />
There was only weak evidence <strong>of</strong> an effect <strong>of</strong> other interventions on MRSA transmission. All TW MRSA strains (21 <strong>of</strong> 21<br />
isolates) and
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
three years and covered 4,349 individuals from 220 randomly selected villages, representing 10.7% <strong>of</strong> all villages in Gabon.<br />
Using a sensitive and specific ELISA method, we found a ZEBOV-specific IgG seroprevalence <strong>of</strong> 15.3% overall, the highest ever<br />
reported. The seroprevalence rate was significantly higher in forested areas (19.4%) than in other ecosystems, namely<br />
grassland (12.4%), savannah (10.5%), and lakeland (2.7%). No other risk factors for seropositivity were found. The specificity <strong>of</strong><br />
anti-ZEBOV IgG was confirmed by Western blot in 138 individuals, and CD8 T cells from seven IgG+ individuals were shown to<br />
produce IFN-γ after ZEBOV stimulation. Together, these findings show that a large fraction <strong>of</strong> the human population living in<br />
forested areas <strong>of</strong> Gabon has both humoral and cellular immunity to ZEBOV. In the absence <strong>of</strong> identified risk factors, the high<br />
prevalence <strong>of</strong> "immune" persons suggests a common source <strong>of</strong> human exposure such as fruits contaminated by bat saliva.<br />
These findings provide significant new insights into ZEBOV circulation and human exposure, and raise important questions as<br />
to the human pathogenicity <strong>of</strong> ZEBOV and the existence <strong>of</strong> natural protective immunization. © 2015 Becquart et al.<br />
No.92<br />
Author (s) : Becquart, P., Wauquier, N., Nkoghe, D., Ndjoyi-Mbiguino, A., Padilla, C., Souris, M., Leroy, E.M.<br />
Title : Acute dengue virus 2 infection in Gabonese patients is associated with an early innate immune response, including strong<br />
interferon alpha production<br />
Source : BMC Infectious Diseases, Volume 10, 17 December <strong>2010</strong>, Article number 356<br />
Document Type : Article<br />
Keywords : alpha interferon, cell surface marker, gamma interferon inducible protein 10, granulocyte colony stimulating<br />
factor, granulocyte macrophage colony stimulating factor, interleukin 17, interleukin 6, monocyte chemotactic protein<br />
1, RANTES, vasculotropin A, chemokine, cytokine, signal peptide; acute disease, adult, aged, article, CD4+ T lymphocyte, CD8+<br />
T lymphocyte, clinical article, controlled study, cytokine production, dengue, Dengue virus 2, ex vivo<br />
study, female, Gabon, human, in vitro study, innate immunity, lymphocyte activation, male, nonhuman, protein blood<br />
level, virus load, virus replication, adaptive immunity, blood, Dengue virus, down regulation, epidemic, flow<br />
cytometry, immunology, middle aged, mononuclear cell, upregulation, virology; Adaptive<br />
Immunity, Adult, Aged, Chemokines, Cytokines, Dengue, Dengue Virus, Disease Outbreaks, Down-Regulation, Female, Flow<br />
Cytometry, Gabon, Humans, Immunity, Innate, Intercellular Signaling Peptides and Proteins, Interferon-alpha, Leukocytes,<br />
Mononuclear, Male, Middle Aged, Up-Regulation, Young Adult<br />
<strong>Abstract</strong> : Background: Dengue is now a leading cause <strong>of</strong> morbidity and mortality throughout the tropics. We conducted the first ex vivo<br />
study <strong>of</strong> dengue fever (DF) in African patients infected during the first Gabonese dengue virus 2 (DENV-2) outbreak in 2007, in<br />
order to investigate cytokine production, including the antiviral cytokine IFN-α, reported to be a potent inhibitor <strong>of</strong> DENV<br />
replication in vitro.Methods: Levels <strong>of</strong> 50 cytokines, chemokines and growth factors were measured in plasma from 36<br />
patients with DENV-2 infection, and in uninfected controls, using Luminex multiplex technology. The results were interpreted<br />
according to the day <strong>of</strong> sampling after symptom onset. PBMC from six patients were also studied for T lymphocyte cell<br />
surface marker expression by flow cytometry.Results: Acute DENV-2 infection elicited high levels <strong>of</strong> several pro-inflammatory<br />
cytokines (IL-6 and IL-17), chemokines (MIF, RANTES, IP-10 and MCP-1) and growth factors (G-CSF, GM-CSF and VEGF-A). We<br />
also observed high levels <strong>of</strong> IFN-α for the first time in adult DF patients, and CD4+ and CD8+ T cell activation at symptom<br />
onset.Conclusion: Acute DENV-2 infection in African patients elicits a strong innate response involving IFN-α production, as<br />
well as an adaptive immune response. © <strong>2010</strong> Becquart et al; licensee BioMed Central Ltd.<br />
No.93<br />
Author (s) : Behmoaras, J., Smith, J., D'Souza, Z., Bhangal, G., Chawanasuntoropoj, R., Tam, F.W.K., Pusey, C.D., Aitman, T.J., Cook, H.T.<br />
Title : Genetic loci modulate macrophage activity and glomerular damage in experimental glomerulonephritis<br />
Source : Journal <strong>of</strong> the American Society <strong>of</strong> Nephrology, Volume 21, Issue 7, July <strong>2010</strong>, Pages 1136-1144<br />
Document Type : Article<br />
Keywords : inducible nitric oxide synthase, tumor necrosis factor alpha; allergic glomerulonephritis, animal cell, animal<br />
experiment, animal model, animal tissue, article, bone marrow transplantation, cell activation, cell infiltration, chromosome<br />
13, chromosome 16, congenic strain, controlled study, genetic susceptibility, immune complex nephritis, kidney<br />
transplantation, Lewis rat, macrophage, male, nonhuman, priority journal, protein expression, quantitative trait<br />
locus, rat, Wistar Kyoto rat; Animals, Bone Marrow Transplantation, Cells, Cultured, Disease Models, Animal, Genetic<br />
Loci, Genetic Predisposition to Disease, Glomerular Mesangium, Glomerulonephritis, Kidney<br />
Transplantation, Macrophages, Male, Matrix Metalloproteinase 12, Nitric Oxide Synthase Type II, Rats, Rats, Inbred Lew, Rats,<br />
Inbred WKY, Tumor Necrosis Factor-alpha<br />
35
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : The Wistar Kyoto (WKY) rat is uniquely susceptible to experimentally induced crescentic glomerulonephritis. Two major<br />
quantitative trait loci (QTLs) on chromosomes 13 (Crgn1) and 16 (Crgn2) with logarithm <strong>of</strong> odds >8, as well as five other loci<br />
(Crgn3 through 7), largely explain this genetic susceptibility. To understand further the effects <strong>of</strong> Crgn1 and Crgn2, we<br />
generated a double-congenic strain by introgressing these loci from glomerulonephritis-resistant Lewis rats onto the WKY<br />
genetic background. Induction <strong>of</strong> nephrotoxic nephritis in the double-congenic rats (WKY.LCrgn1,2) produced markedly fewer<br />
glomerular crescents, reduced macrophage infiltration, and decreased expression <strong>of</strong> glomerular TNF-α and inducible nitric<br />
oxide synthase expression compared with control animals. Bone marrow and kidney transplantation studies between parental<br />
and WKY.LCrgn1,2 strains, together with in vitro experiments, demonstrated that Crgn1 and Crgn2 contribute exclusively to<br />
circulating cell-related glomerular injury by regulating macrophage infiltration and activation. The residual genetic<br />
susceptibility to crescentic glomerulonephritis in WKY.LCrgn1,2 rats associated with macrophage activity (especially with<br />
enhanced metalloelastase expression) rather than macrophage infiltration. Taken together, these results demonstrate that a<br />
genetic influence on macrophage activation, rather than number, determines glomerular damage in immune-mediated<br />
glomerulonephritis. Copyright © <strong>2010</strong> by the American Society <strong>of</strong> Nephrology.<br />
No.94<br />
Author (s) : Benjapibal, M., Chaopotong, P., Leelaphatanadit, C., Jaishuen, A.<br />
Title : Ruptured ovarian endodermal sinus tumor diagnosed during pregnancy: Case report and review <strong>of</strong> the literature<br />
Source : Journal <strong>of</strong> Obstetrics and Gynaecology Research, Volume 36, Issue 5, October <strong>2010</strong>, Pages 1137-1141<br />
Document Type : Article<br />
Keywords : endodermal sinus tumor; ovarian cancer; pregnancy; ruptured<br />
<strong>Abstract</strong> : Pregnancy complicated by endodermal sinus tumor <strong>of</strong> the ovary is a very rare event. We present a case report <strong>of</strong> a pregnant<br />
woman with a ruptured primary endodermal sinus tumor <strong>of</strong> the ovary that was diagnosed at 13 weeks <strong>of</strong> gestation. After right<br />
salpingo-oophorectomy had been performed the patient received four cycles <strong>of</strong> combination chemotherapy (bleomycin,<br />
etoposide, and cisplatin) starting at the 15th week <strong>of</strong> pregnancy. The patient did not have any evidence <strong>of</strong> tumor recurrence<br />
for 23 months. Her baby did not have any evidence <strong>of</strong> malformations and showed normal growth and development at 21<br />
months <strong>of</strong> follow up. © <strong>2010</strong> Japan Society <strong>of</strong> Obstetrics and Gynecology.<br />
No.95<br />
Author (s) : Benner, M.T., Townsend, J., Kaloi, W., Htwe, K., Naranichakul, N., Hunnangkul, S., Carrara, V.I., Sondorp, E.<br />
Title : Reproductive health and quality <strong>of</strong> life <strong>of</strong> young Burmese refugees in Thailand<br />
Source : Conflict and Health, Volume 4, Issue 1, <strong>2010</strong>, Article number 5<br />
Document Type : Article<br />
Keywords : –<br />
<strong>Abstract</strong> : Background: Of the 140 000 Burmese* refugees living in camps in Thailand, 30% are youths aged 15-24. Health services in<br />
these camps do not specifically target young people and their problems and needs are poorly understood. This study aimed<br />
to assess their reproductive health issues and quality <strong>of</strong> life, and identifies appropriate service needs. Methods. We used a<br />
stratified two-stage random sample questionnaire survey <strong>of</strong> 397 young people 15-24 years from 5,183 households, and 19<br />
semi-structured qualitative interviews to assess and explore health and quality <strong>of</strong> life issues. Results: The young people in the<br />
camps had very limited knowledge <strong>of</strong> reproductive health issues; only about one in five correctly answered at least one<br />
question on reproductive health. They were clear that they wanted more reproductive health education and services, to be<br />
provided by health workers rather than parents or teachers who were not able to give them the information they needed.<br />
Marital status was associated with sexual health knowledge; having relevant knowledge <strong>of</strong> reproductive health was up to six<br />
times higher in married compared to unmarried youth, after adjusting for socio-economic and demographic factors. Although<br />
condom use was considered important, in practice a large proportion <strong>of</strong> respondents felt too embarrassed to use them.<br />
There was a contradiction between moral views and actual behaviour; more than half believed they should remain virgins<br />
until marriage, while over half <strong>of</strong> the youth experienced sex before marriage. Two thirds <strong>of</strong> women were married before the<br />
age <strong>of</strong> 18, but two third felt they did not marry at the right age. Forced sex was considered acceptable by one in three youth.<br />
The youth considered their quality <strong>of</strong> life to be poor and limited due to confinement in the camps, the limited work<br />
opportunities, the aid dependency, the unclear future and the boredom and unhappiness they face. Conclusions: The long<br />
conflict in Myanmar and the resultant long stay in refugee camps over decades affect the wellbeing <strong>of</strong> these young people.<br />
Lack <strong>of</strong> sexual health education and relevant services, and their concerns for their future are particular problems, which need<br />
36
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
to be addressed. Issues <strong>of</strong> education, vocational training and job possibilities also need to be considered. *Burmese is used<br />
for all ethnic groups. © <strong>2010</strong> Benner et al; licensee BioMed Central Ltd.<br />
No.96<br />
Author (s) : Bhakdi, S.C., Ottinger, A., Somsri, S., Sratongno, P., Pannadaporn, P., Chimma, P., Malasit, P., Pattanapanyasat, K., Neumann,<br />
H.P.<br />
Title : Optimized high gradient magnetic separation for isolation <strong>of</strong> Plasmodium-infected red blood cells<br />
Source: : Malaria Journal, Volume 9, Issue 1, <strong>2010</strong>, Article number 38<br />
Document Type : Article<br />
Keywords : polymer, polypropylene, stainless steel; article, blood culture, cell isolation, cell synchronization, cell viability, comparative<br />
study, density, dipole, erythrocyte, flow cytometry, high gradient magnetic separation, infection rate, magnetic field, magnetic<br />
separation, malaria, material coating, microscopy, nonhuman, Plasmodium<br />
falciparum, purification, schizont, syringe, viscosity, animal, blood, cell adhesion, cell separation, erythrocyte<br />
aggregation, growth, development and aging, immunology, instrumentation, isolation and<br />
purification, magnetism, methodology, osmotic fragility, parasitology, particle size, physiology, Plasmodium; Animals, Cell<br />
Adhesion, Cell Separation, Erythrocyte Aggregation, Erythrocytes, Flow Cytometry, Magnetics, Malaria, Osmotic<br />
Fragility, Particle Size, Plasmodium, Plasmodium falciparum, Schizonts<br />
<strong>Abstract</strong> : Background. Highly purified infected red blood cells (irbc), or highly synchronized parasite cultures, are regularly required in<br />
malaria research. Conventional isolation and synchronization rely on density and osmotic fragility <strong>of</strong> irbc, respectively. High<br />
gradient magnetic separation (HGMS) <strong>of</strong>fers an alternative based on intrinsic magnetic properties <strong>of</strong> irbc, avoiding exposure to<br />
chemicals and osmotic stress. Successful HGMS concentration in malaria research was previously reported using polymer<br />
coated columns, while HGMS depletion has not been described yet. This study presents a new approach to both HGMS<br />
concentration and depletion in malaria research, rendering polymer coating unnecessary. Methods. A dipole magnet<br />
generating a strong homogenous field was custom assembled. Polypropylene syringes were fitted with one-way stopcocks<br />
and filled with stainless steel wool. Rbc from Plasmodium falciparum cultures were resuspended in density and viscosity<br />
optimized HGMS buffers and HGMS processed. Purification and depletion results were analysed by flow cytometer and light<br />
microscopy. Viability was evaluated by calculating the infection rate after re-culturing <strong>of</strong> isolates. Results. In HGMS<br />
concentration, purity <strong>of</strong> irbc isolates from asynchronous cultures consistently ranged from 94.8% to 98.4% (mean 95.7%).<br />
With further optimization, over 90% <strong>of</strong> isolated irbc contained segmented schizonts. Processing time was less than 45 min.<br />
Reinfection rates ranged from 21.0% to 56.4%. In HGMS depletion, results were comparable to treatment with sorbitol, as<br />
demonstrated by essentially identical development <strong>of</strong> cultures. Conclusion. The novel HGMS concentration procedure<br />
achieves high purities <strong>of</strong> segmented stage irbc from standard asynchronous cultures, and is the first HGMS depletion<br />
alternative to sorbitol lysis. It represents a simple and highly efficient alternative to conventional irbc concentration and<br />
synchronization methods. © <strong>2010</strong> Bhakdi et al; licensee BioMed Central Ltd.<br />
No.97<br />
Author (s) : Bhumiratana, A., Pechgit, P., Koyadun, S., Siriaut, C., Yongyuth, P.<br />
Title : Imported bancr<strong>of</strong>tian filariasis: Diethylcarbamazine response and benzimidazole susceptibility <strong>of</strong> Wuchereria bancr<strong>of</strong>ti in<br />
dynamic cross-border migrant population targeted by the National Program to Eliminate Lymphatic Filariasis in South Thailand<br />
Source : Acta Tropica, Volume 113, Issue 2, February <strong>2010</strong>, Pages 121-128<br />
Document Type : Article<br />
Keywords : Benzimidazole susceptibility; Diethylcarbamazine; Imported bancr<strong>of</strong>tian filariasis; National Program to Eliminate Lymphatic<br />
Filariasis; Wuchereria bancr<strong>of</strong>ti<br />
<strong>Abstract</strong> : The implementation on the Thailand-Myanmar border <strong>of</strong> annual mass drug administration (MDA) <strong>of</strong> a single 6 mg/kg dose <strong>of</strong><br />
diethylcarbamazine (DEC) plus 400 mg albendazole, part <strong>of</strong> the National Program to Eliminate Lymphatic Filariasis (PELF), has<br />
been challenging. In particular, chain migration <strong>of</strong> cross-border Myanmar workers at risk for nocturnally periodic Wuchereria<br />
bancr<strong>of</strong>ti infection can lead to imported bancr<strong>of</strong>tian filariasis (IBF) in Thailand. IBF is targeted for multiple-dose MDA with 300<br />
mg DEC, in addition to what is recommended by the World Health Organization (WHO). The dynamic Myanmar migrants in<br />
Phang-nga, southern Thailand were sampled to test whether the responsible W. bancr<strong>of</strong>ti has a genetic predisposition <strong>of</strong><br />
benzimidazole exposure, and IBF exhibits DEC susceptibility. The long-term migrants had more access to DEC. IBF in W.<br />
bancr<strong>of</strong>ti antigenemic (micr<strong>of</strong>ilaremic vs. amicr<strong>of</strong>ilaremic) short-term migrants exhibited susceptibility to a 300-mg single-dose<br />
DEC treatment. During the course <strong>of</strong> a 3-month follow-up, antigenemia was significantly reduced, but micr<strong>of</strong>ilaremia was<br />
37
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
fluctuated. Surprisingly, a newly recognized Mansonella infection co-existing among W. bancr<strong>of</strong>ti-affected Myanmar migrants<br />
elicited micr<strong>of</strong>ilaremia clearance within a month after treatment. As a result <strong>of</strong> the presence <strong>of</strong> genetically stable W. bancr<strong>of</strong>ti<br />
β-tubulin (Wbtubb) gene responsible for benzimidazole susceptibility, IBF did not possess a genetic predisposition for<br />
benzimidazole exposure. Point mutations at positions Phe167Tyr and Phe200Tyr were not detected by Wbtubb locus-specific<br />
nested PCR and sequencing. This study has the potential to help guide not only the Thai/Myanmar PELF surveillance and<br />
monitoring <strong>of</strong> mass treatment impacts on W. bancr<strong>of</strong>ti, but also the other endemic countries allied with the Global Program<br />
to Eliminate Lymphatic Filariasis (GPELF). © 2009 Elsevier B.V. All rights reserved.<br />
No.98<br />
Author (s) : Bienvenu, F., Jirawatnotai, S., Elias, J.E., Meyer, C.A., Mizeracka, K., Marson, A., Frampton, G.M., Cole, M.F., Odom, D.T., Odajima,<br />
J., Geng, Y., Zagozdzon, A., Jecrois, M., Young, R.A., Liu, X.S., Cepko, C.L., Gygi, S.P., Sicinski, P.<br />
Title : Transcriptional role <strong>of</strong> cyclin D1 in development revealed by a genetic-proteomic screen<br />
Source : Nature, Volume 463, Issue 7279, 21 January <strong>2010</strong>, Pages 374-378<br />
Document Type : Article<br />
Keywords : binding protein, cyclic AMP responsive element binding protein binding protein, cyclin D1, hemagglutinin, histone<br />
acetyltransferase, Notch1 receptor; cancer, DNA, genetic analysis, genome, proteomics; acetylation, allele, animal<br />
experiment, animal tissue, article, cell cycle, cell proliferation, chromatin immunoprecipitation, DNA<br />
microarray, embryo, female, gene expression, in vivo study, knockout mouse, mass spectrometry, mouse, mouse<br />
strain, nonhuman, nucleotide sequence, phenotype, priority journal, protein function, retina, reverse transcription polymerase<br />
chain reaction, stem cell, upregulation; Alleles, Animals, Chromatin Immunoprecipitation, CREB-Binding Protein, Cyclin<br />
D1, Gene Expression Regulation, Developmental, Genome, High-Throughput Screening Assays, Histone Acetyltransferases, Mass<br />
Spectrometry, Mice, Oligonucleotide Array Sequence Analysis, Promoter Regions, Genetic, Protein<br />
Binding, Proteomics, Rats, Receptor, Notch1, Retina, Stem Cells, Transcription, Genetic<br />
<strong>Abstract</strong> : Cyclin D1 belongs to the core cell cycle machinery, and it is frequently overexpressed in human cancers. The full repertoire<br />
<strong>of</strong> cyclin D1 functions in normal development and oncogenesis is unclear at present. Here we developed Flag-and<br />
haemagglutinin-tagged cyclin D1 knock-in mouse strains that allowed a high-throughput mass spectrometry approach to<br />
search for cyclin D1-binding proteins in different mouse organs. In addition to cell cycle partners, we observed several<br />
proteins involved in transcription. Genome-wide location analyses (chromatin immunoprecipitation coupled to DNA<br />
microarray; ChIP-chip) showed that during mouse development cyclin D1 occupies promoters <strong>of</strong> abundantly expressed genes.<br />
In particular, we found that in developing mouse retinasan organ that critically requires cyclin D1 functioncyclin D1 binds the<br />
upstream regulatory region <strong>of</strong> the Notch1 gene, where it serves to recruit CREB binding protein (CBP) histone<br />
acetyltransferase. Genetic ablation <strong>of</strong> cyclin D1 resulted in decreased CBP recruitment, decreased histone acetylation <strong>of</strong> the<br />
Notch1 promoter region, and led to decreased levels <strong>of</strong> the Notch1 transcript and protein in cyclin D1-null (Ccnd1-/-) retinas.<br />
Transduction <strong>of</strong> an activated allele <strong>of</strong> Notch1 into Ccnd1-/-retinas increased proliferation <strong>of</strong> retinal progenitor cells, indicating<br />
that upregulation <strong>of</strong> Notch1 signalling alleviates the phenotype <strong>of</strong> cyclin D1-deficiency. These studies show that in addition to<br />
its well-established cell cycle roles, cyclin D1 has an in vivo transcriptional function in mouse development. Our approach,<br />
which we term genetic-proteomic, can be used to study the in vivo function <strong>of</strong> essentially any protein. © <strong>2010</strong> Macmillan<br />
Publishers Limited. All rights reserved.<br />
No.99<br />
Author (s) : Bish, E.K., Suwandechochai, R.<br />
Title : Optimal capacity for substitutable products under operational postponement<br />
Source : European Journal <strong>of</strong> Operational Research, Volume 207, Issue 2, 1 December <strong>2010</strong>, Pages 775-783<br />
Document Type : Article<br />
Keywords : Capacity planning; Flexible capacity; Price/quantity postponement; Pricing; Product substitution; Uncertainty<br />
<strong>Abstract</strong> : We consider a monopolist producing two substitutable products with one flexible (shared) capacity. The demand <strong>of</strong> each<br />
product is a linear function <strong>of</strong> the prices <strong>of</strong> both products, and is subject to an additive shock. We study the impact <strong>of</strong> two<br />
key drivers, namely the degree <strong>of</strong> substitution between the products and the level <strong>of</strong> operational postponement, on the<br />
optimal capacity and the resulting expected pr<strong>of</strong>it. We show that the relationship between the optimal capacity and the<br />
degree <strong>of</strong> product substitution is not impacted by the different postponement strategies the firm can utilize or by the<br />
different settings (forced clearance versus holdback) considered in the previous literature. On the other hand, how capacity is<br />
affected by postponement critically depends on how closely substitutable the products are. In particular, we show that the<br />
38
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
well-known result that operational postponement and capacity are strategic complements in a single-product setting (Van<br />
Mieghem and Dada, 1999) no longer holds in our setting, because the two substitutable products are now linked through<br />
consumer-driven substitution, which the firm can influence through pricing. In particular, capacity and operational<br />
postponement (in the form <strong>of</strong> quantity postponement) can be either strategic substitutes or strategic complements, and this<br />
depends on both the firm's cost structure and the degree <strong>of</strong> substitution between the products. We also study the impact <strong>of</strong><br />
forced clearance on the firm's expected pr<strong>of</strong>it and find that clearance deteriorates the firm's earnings more when the<br />
products it <strong>of</strong>fers are highly differentiated. © <strong>2010</strong> Elsevier B.V. All rights reserved.<br />
No.100<br />
Author (s) : Blacksell, S.D., Jenjaroen, K., Phetsouvanh, R., Tanganuchitcharnchai, A., Phouminh, P., Phongmany, S., Day, N.P.J., Newton, P.N.<br />
Title : Accuracy <strong>of</strong> rapid IgM-based immunochromatographic and immunoblot assays for diagnosis <strong>of</strong> acute scrub typhus and murine<br />
typhus infections in Laos<br />
Source : American Journal <strong>of</strong> Tropical Medicine and Hygiene, Volume 83, Issue 2, August <strong>2010</strong>, Pages 365-369<br />
Document Type : Article<br />
Keywords : immunoglobulin M; adult, antibody titer, article, blood analysis, controlled study, diagnostic accuracy, female, fever, hospital<br />
admission, human, immunoblotting, immun<strong>of</strong>luorescence, Laos, major clinical study, male, Rickettsia typhi, sensitivity and<br />
specificity, typhus; Acute<br />
Disease, Adolescent, Adult, Child, Chromatography, Humans, Immunoassay, Immunoblotting, Immunoglobulin M, Orientia<br />
tsutsugamushi, Rickettsia typhi, Scrub Typhus, Sensitivity and Specificity, Typhus, Endemic Flea-Borne, Young Adult;<br />
Murinae, Orientia tsutsugamushi, Rickettsia typhi<br />
<strong>Abstract</strong> : We studied the diagnostic accuracy <strong>of</strong> a rapid immunochromatographic test (ICT) for detection <strong>of</strong> IgM against scrub typhus (ST<br />
ICT) and an immunoblot test for the detection <strong>of</strong> IgM against murine typhus (MT IBT) by using admission serum samples from<br />
1,030 febrile patients in Laos. Sensitivity and specificity for the ST ICT determined by using the diagnostic criteria <strong>of</strong> a four-fold<br />
increase in IgM against Orientia tsutsugamushi between paired samples were 23.8% (95% confidence interval [CI] = 15.9-<br />
33.3%) and 86.2% (95% CI = 84.1-88.6%), respectively. Sensitivity and specificity for the ST ICT determined by using an<br />
admission IgM titer ≥ 1:400 were 39.1% (95% CI = 34.1-44.2%) and 99.5% (95% CI = 98,7-99.9%), respectively. Sensitivity and<br />
specificity for the MT IBT determined by using the criteria <strong>of</strong> a four-fold increase in IgM against Rickettsia typhi between paired<br />
serum samples were 61.2% (95% CI = 53.7-68.3%) and 86.5% (95% CI = 84.1-88.8%), respectively. Sensitivity and specificity for<br />
the MT IBT determined by using an admission IgM titer ≥ 1:400 were 54.6% (95% CI = 49.1-60.0%) and 94.1 % (95% CI = 92.0-<br />
95.7%). respectively. Both assays had relatively good specificity but low sensitivity and thus have limited utility for admission<br />
diagnosis. Copyright © <strong>2010</strong> by The American Society <strong>of</strong> Tropical Medicine and Hygiene.<br />
No.101<br />
Author (s) : Blacksell, S.D., Jenjaroen, K., Phetsouvanh, R., Wuthiekanun, V., Day, N.P.J., Newton, P.N., Ching, W.-M.<br />
Title : Accuracy <strong>of</strong> accessbio immunoglobulin M and total antibody rapid immunochromatographic assays for the diagnosis <strong>of</strong> acute<br />
scrub typhus infection<br />
Source : Clinical and Vaccine Immunology, Volume 17, Issue 2, February <strong>2010</strong>, Pages 263-266<br />
Document Type : Article<br />
Keywords : immunoglobulin M antibody; article, bacterium detection, controlled study, dengue, diagnostic<br />
procedure, fever, human, Laos, leptospirosis, malaria, melioidosis, nonhuman, Orientia tsutsugamushi, priority journal, rapid<br />
immunochromatographic test, scrub typhus, sensitivity and specificity; Antibodies,<br />
Bacterial, Humans, Immunoassay, Immunoglobulin M, Laos, Orientia tsutsugamushi, Scrub Typhus, Sensitivity and Specificity<br />
<strong>Abstract</strong> : Using archived samples, we assessed the diagnostic capacity <strong>of</strong> a rapid immunochromatographic test (ICT) for the detection <strong>of</strong><br />
Orientia tsutsugamushi IgM and total antibodies to aid with the diagnosis <strong>of</strong> acute scrub typhus infection in febrile patients in<br />
Laos. The sensitivity and the specificity <strong>of</strong> the ICT for the detection <strong>of</strong> IgM were 96.8% (121/125 samples; 95% confidence<br />
interval [CI], 92.1 to 99.1%) and 93.3% (98/105 samples; 95% CI, 86.7 to 97.3%), respectively. For the detection <strong>of</strong> total<br />
antibodies, the sensitivity was 97.6% (122/125 samples; 95% CI, 93.1 to 99.5%), but the specificity was much lower, at 71.4%<br />
(75/105 samples; 95% CI, 61.8 to 79.8%). Copyright © <strong>2010</strong>, American Society for Microbiology. All Rights Reserved.<br />
No.102<br />
Author (s) : Blessborn, D., Romsing, S., Bergqvist, Y., Lindegardh, N.<br />
39
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Assay for screening for six antimalarial drugs and one metabolite using dried blood spot sampling, sequential extraction and<br />
ion-trap detection<br />
Source : Bioanalysis, Volume 2, Issue 11, November <strong>2010</strong>, Pages 1839-1847<br />
Document Type : Article<br />
Keywords : benflumetol, chloroquine, deethylchloroquine, mefloquine, pyrimethamine, quinine, sulfadoxine, antimalarial agent;<br />
article, blood sampling, controlled study, dried blood spot sampling, drug determination, drug screening, drug structure, drug<br />
use, ion trap mass spectrometry, limit <strong>of</strong> detection, liquid chromatography, physical chemistry, reliability, solid phase<br />
extraction, validation process, blood analysis, desiccation, fractionation, human, isolation and purification, mass<br />
spectrometry, metabolism, methodology, time; Antimalarials, Blood Chemical Analysis, Blood Specimen Collection, Chemical<br />
Fractionation, Chromatography, Liquid, Desiccation, Humans, Mass Spectrometry, Time Factors<br />
<strong>Abstract</strong> : Background: More parasites are becoming resistant to antimalarial drugs, and in many areas a change in first-line drug<br />
treatment is necessary. The aim <strong>of</strong> the developed assay is to help determine drug use in these areas and also to be a<br />
complement to interviewing patients, which will increase reliability <strong>of</strong> surveys. Results: This assay detects quinine,<br />
mefloquine, sulfadoxine, pyrimethamine, lumefantrine, chloroquine and its metabolite desethylchloroquine in a 100-μl dried<br />
blood spot. Most <strong>of</strong> the drugs also have long half-lives that make them detectable at least 7 days after administration. The<br />
drugs are extracted from the dried blood spot with sequential extraction (due to the big differences in physicochemical<br />
properties), solid-phase extraction is used as sample clean-up and separation is performed with gradient-LC with MS ion-trap<br />
detection. Conclusion: Detection limits (S/N > 5:1) at 50 ng/ml or better were achieved for all drugs except lumefantrine (200<br />
ng/ml), and thus can be used to determine patient compliance. A major advantage <strong>of</strong> using the ion-trap MS it that it will be<br />
possible to go back into the data and look for other drugs as needed. © <strong>2010</strong> Future Science Ltd.<br />
No.103<br />
Author (s) : Boel, M., Carrara, V.I., Rijken, M., Proux, S., Nacher, M., Pimanpanarak, M., Paw, M.K., Moo, O., Gay, H., Bailey, W., Singhasivanon,<br />
P., White, N.J., Nosten, F., Mcgready, R.<br />
Title : Complex interactions between soil-transmitted helminths and malaria in pregnant women on the thai-burmese border<br />
Source : PLoS Neglected Tropical Diseases, Volume 4, Issue 11, November <strong>2010</strong>, Article number e887<br />
Document Type : Article<br />
Keywords : adolescent, adult, anemia, article, ascariasis, Ascaris lumbricoides, blood smear, controlled study, disease association, disease<br />
severity, feces analysis, female, health survey, helminthiasis, hematocrit, hookworm infection, human, infection risk, intestine<br />
infection, low birth weight, major clinical study, malaria, Myanmar, Plasmodium vivax malaria, pregnancy, pregnancy<br />
outcome, pregnant woman, prenatal care, soil micr<strong>of</strong>lora, Thailand, Trichuris, animal, cross-sectional<br />
study, feces, health, helminth, isolation and purification, parasitology, Plasmodium, pregnancy complication, prevalence, risk<br />
factor, soil; Adolescent, Adult, Anemia, Animals, Cross-Sectional<br />
Studies, Feces, Female, Helminthiasis, Helminths, Humans, Malaria, Myanmar, Plasmodium, Pregnancy, Pregnancy<br />
Complications, Parasitic, Prevalence, Risk Factors, Rural Health, Soil, Thailand, Young Adult<br />
<strong>Abstract</strong> : Background: Deworming is recommended by the WHO in girls and pregnant and lactating women to reduce anaemia in areas<br />
where hookworm and anaemia are common. There is conflicting evidence on the harm and the benefits <strong>of</strong> intestinal<br />
geohelminth infections on the incidence and severity <strong>of</strong> malaria, and consequently on the risks and benefits <strong>of</strong> deworming in<br />
malaria affected populations. We examined the association between geohelminths and malaria in pregnancy on the Thai-<br />
Burmese border. Methodology: Routine antenatal care (ANC) included active detection <strong>of</strong> malaria (weekly blood smear) and<br />
anaemia (second weekly haematocrit) and systematic reporting <strong>of</strong> birth outcomes. In 1996 stool samples were collected in<br />
cross sectional surveys from women attending the ANCs. This was repeated in 2007 when malaria incidence had reduced<br />
considerably. The relationship between geohelminth infection and the progress and outcome <strong>of</strong> pregnancy was assessed.<br />
Principal Findings: Stool sample examination (339 in 1996, 490 in 2007) detected a high prevalence <strong>of</strong> geohelminths<br />
70%(578/829), including hookworm (42.8% (355)), A. lumbricoides (34.4% (285)) and T.trichuria (31.4% (250)) alone or in<br />
combination. A lower proportion <strong>of</strong> women (829) had mild (21.8% (181)) or severe (0.2% (2)) anaemia, or malaria 22.4% (186)<br />
(P.vivax monoinfection 53.3% (101/186)). A. lumbricoides infection was associated with a significantly decreased risk <strong>of</strong> malaria<br />
(any species) (AOR: 0.43, 95% CI: 0.23-0.84) and P.vivax malaria (AOR: 0.29, 95% CI: 0.11-0.79) whereas hookworm infection was<br />
associated with an increased risk <strong>of</strong> malaria (any species) (AOR: 1.66, 95% CI: 1.06-2.60) and anaemia (AOR: 2.41, 95% CI: 1.18-<br />
4.93). Hookworm was also associated with low birth weight (AOR: 1.81, 95% CI: 1.02-3.23). Conclusion/Significance: A.<br />
lumbricoides and hookworm appear to have contrary associations with malaria in pregnancy. © <strong>2010</strong> Boel et al.<br />
40
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.104<br />
Author (s) : Boonaiam, S., Chaiprasert, A., Prammananan, T., Leechawengwongs, M.<br />
Title : Genotypic analysis <strong>of</strong> genes associated with isoniazid and ethionamide resistance in MDR-TB isolates from Thailand.<br />
Source : Clinical microbiology and infection : the <strong>of</strong>ficial publication <strong>of</strong> the European Society <strong>of</strong> Clinical Microbiology and Infectious<br />
Diseases, Volume 16, Issue 4, April <strong>2010</strong>, Pages 396-399<br />
Document Type : Article<br />
Keywords : bacterial protein, catalase, ethionamide, isoniazid, katG protein, Mycobacterium tuberculosis, tuberculostatic agent;<br />
article, genetics, genotype, isolation and purification, microbiology, multidrug resistance, multidrug resistant<br />
tuberculosis, Mycobacterium tuberculosis, sequence analysis, Thailand; Antitubercular Agents, Bacterial<br />
Proteins, Catalase, Drug Resistance, Multiple, Bacterial, Ethionamide, Genotype, Isoniazid, Mycobacterium<br />
tuberculosis, Sequence Analysis, Thailand, Tuberculosis, Multidrug-Resistant<br />
<strong>Abstract</strong> : Nucleotide sequences <strong>of</strong> genes conferring isoniazid resistance (katG, inhA, oxyR-ahpC and ndh) and ethionamide resistance<br />
(ethA) in 160 drug-resistant Mycobacterium tuberculosis clinical isolates from Thailand were analysed. Mutations in the katG<br />
gene were found in 129 isolates, predominantly at codon 315, which was mutated in 127 isolates. Twenty-two isolates had<br />
mutations in the inhA promoter and coding region. Mutations in the oxyR-ahpC intergenic region and in ndh were detected in<br />
four and one isolate(s), respectively. Of 24 ethionamide-resistant isolates, 13 had mutations in the ethA gene. However, these<br />
mutations were dispersed along the entire gene, with no codon predominating significantly.<br />
No.105<br />
Author (s) : Boonbaichaiyapruck, S., Pienvichit, P., Limpijarnkij, T., Rerkpattanapipat, P., Pongpatananurak, A., Lee, R., Ungkanont, A., Hong-<br />
Eng, S.<br />
Title : Transcoronary infusion <strong>of</strong> bone marrow derived multipotent stem cells to preserve left ventricular geometry and function<br />
after myocardial infarction<br />
Source : Clinical Cardiology, Volume 33, Issue 7, July <strong>2010</strong>, Pages E10-E15<br />
Document Type : Article<br />
Keywords : gadolinium; adult, article, balloon, clinical article, controlled study, coronary artery, female, geometry, heart<br />
hemodynamics, heart infarction, heart left ventricle ejection fraction, heart left ventricle function, heart<br />
preservation, hematopoietic stem cell, human, intracoronary infusion, male, multipotent stem cell, nuclear magnetic<br />
resonance imaging, ST segment elevation, survival rate, systolic blood pressure; Aged, Bone Marrow Transplantation, Contrast<br />
Media, Feasibility Studies, Female, Gadolinium DTPA, Heart Ventricles, Humans, Magnetic Resonance Imaging,<br />
Cine, Male, Middle Aged, Multipotent Stem Cells, Myocardial Infarction, Pilot Projects, Stroke Volume, Thailand, Time<br />
Factors, Treatment Outcome, Ventricular Function, Left, Ventricular Remodeling<br />
<strong>Abstract</strong> : Objectives: Myocardial damage aftermyocardial infarction(MI)was deemed irreversible after late reperfusion.<br />
Administration<strong>of</strong>multipotent stem cells (MSC) into such infarctmay regenerate themyocardiumand capillary network. The<br />
objectives were to study the feasibility and safety <strong>of</strong> MSC infusion and its effect on infarct size and left ventricular (LV)<br />
function. Methods: We conducted a pilot study in patients who survived ST-elevation MI with late reperfusion therapy and<br />
remained hemodynamically stable. Bone marrow derived MSC was infused into a patent infarct-related coronaryartery during<br />
brief lowpressure (2 atm) balloon inflation. A 3-T gadolinium-based MRI was performed at baseline and 8 weeks later to<br />
evaluate infarct area and LV function. Results: We enrolled 10 patients, age 63.8±2.8 years 5.2±4.12 × 106 MSC were infused<br />
via coronary artery 24.8±16 days after infarction. The procedures were successful in all patients without any in-hospital event.<br />
Infarct size by MRI decreased by 5.84% (P = .018) over 8 weeks. Mean baseline left ventricular ejection fraction (LVEF) was<br />
44.1% ± 9% and was 46.3%±9% at 8 weeks (P = .34). A trend <strong>of</strong> smaller LV end-systolic volumewith 65.02±18.2 ml<br />
vs63.04±21.89 ml (P = .09)with no change <strong>of</strong> LV end-diastolic volume observed. Conclusion: MSC infusion into coronary<br />
circulation was feasible and safe after myocardial infarction. Infarct size was reduced with preservation <strong>of</strong> LV geometry. ©<br />
<strong>2010</strong>Wiley Periodicals, Inc.<br />
No.106<br />
Author (s) : Boonchai, W., Iamtharachai, P.<br />
Title : The pH <strong>of</strong> commonly available soaps, liquid cleansers, detergents and alcohol gels<br />
Source : Dermatitis, Volume 21, Issue 3, June <strong>2010</strong>, Pages 154-156<br />
Document Type : Article<br />
41
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : alcohol, alcohol gel, detergent, disinfectant agent, liquid cleanser, soap, tap water, unclassified drug;<br />
acidity, alkalinity, article, cleaning, clinical assessment, clinical evaluation, clinical protocol, concentration<br />
(parameters), controlled study, dilution, disinfection, dissolution, gel, hand washing, human, laundry, market, pH, pH<br />
measurement, practice guideline, priority journal, skin, skin decontamination; Anti-Infective Agents,<br />
Local, Detergents, Ethanol, Gels, Hand, Handwashing, Humans, Hydrogen-Ion Concentration, Soaps<br />
<strong>Abstract</strong> : Background: The hydrogen ion concentration (pH) <strong>of</strong> a cleanser certainly has an impact on skin condition. Dermatologists<br />
always need to recommend a cleanser to patients with hand dermatitis or sensitive skin; particularly during the outbreak <strong>of</strong><br />
swine (AH1N1 virus) influenza, frequent hand washing and alcohol gel cleansing were greatly recommended. Objectives: The<br />
purpose <strong>of</strong> this study was to evaluate the pH <strong>of</strong> various commonly available cleansers and alcohol gels on the market to<br />
assess patient comfort in using such products and to make good recommendations to our patients. Methods: Multiple brands<br />
<strong>of</strong> liquid cleansers, dishwashing liquids, soaps, laundry detergents, and alcohol gels commonly available on the market were<br />
assessed for pH by using a pH meter and pH-indicator strips. The pH assessment imitated real-life conditions by diluting each<br />
cleanser with tap water and then comparing the changed pH. Results: The pH levels <strong>of</strong> liquid cleansers, dishwashing liquids, a<br />
beauty bar, and alcohol gels were acidic to neutral and compatible with normal skin pH. Most bar soaps, baby soaps, and<br />
powdered laundry detergents had a pH in the alkali range. The pH <strong>of</strong> concentrated cleansers was slightly different from that<br />
<strong>of</strong> their dissolved forms. Conclusion: Regarding the antiseptic property and pH <strong>of</strong> the cleansers, alcohol gels with moisturizers<br />
appeared to be the best hand cleansers to recommend to our patients. © <strong>2010</strong> American Contact Dermatitis Society. All<br />
Rights Reserved.<br />
No.107<br />
Author (s) : Boonchird, C., Mahapanichkul, T., Cherdshewasart, W.<br />
Title : Differential binding with ERα and ERβ <strong>of</strong> the phytoestrogen-rich plant Pueraria mirifica<br />
Source : Brazilian Journal <strong>of</strong> Medical and Biological Research, Volume 43, Issue 2, February <strong>2010</strong>, Pages 195-200<br />
Document Type : Article<br />
Keywords : Estrogen receptor α (ERα); Estrogen receptor β (ERβ); Is<strong>of</strong>lavonoid; Phytoestrogen; Pueraria mirifica; YES assay<br />
<strong>Abstract</strong> : Variations in the estrogenic activity <strong>of</strong> the phytoestrogen-rich plant, Pueraria mirifica, were determined with yeast estrogen<br />
screen (YES) consisting <strong>of</strong> human estrogen receptors (hER) hERα and hERβ and human transcriptional intermediary factor 2<br />
(hTIF2) or human steroid receptor coactivator 1 (hSRC1), respectively, together with the β-galactosidase expression cassette.<br />
Relative estrogenic potency was expressed by determining the β-galactosidase activity (EC50) <strong>of</strong> the tuber extracts in relation<br />
to 17β-estradiol. Twenty-four and 22 <strong>of</strong> the plant tuber ethanolic extracts interacted with hERα and hERβ, respectively, with<br />
a higher relative estrogenic potency with hERβ than with hERα. Antiestrogenic activity <strong>of</strong> the plant extracts was also<br />
determined by incubation <strong>of</strong> plant extracts with 17β-estradiol prior to YES assay. The plant extracts tested exhibited<br />
antiestrogenic activity. Both the estrogenic and the antiestrogenic activity <strong>of</strong> the tuber extracts were metabolically activated<br />
with the rat liver S9-fraction prior to the assay indicating the positive influence <strong>of</strong> liver enzymes. Correlation analysis between<br />
estrogenic potency and the five major is<strong>of</strong>lavonoid contents within the previously HPLC-analyzed tuberous samples namely<br />
puerarin, daidzin, genistin, daidzein, and genistein revealed a negative result.<br />
No.108<br />
Author (s) : Boondireke, S., Mungthin, M., Tan-ariya, P., Boonyongsunchai, P., Naaglor, T., Wattanathum, A., Treewatchareekorn, S.,<br />
Leelayoova, S.<br />
Title : Evaluation <strong>of</strong> sensitivity <strong>of</strong> multiplex PCR for detection <strong>of</strong> Mycobacterium tuberculosis and Pneumocystis jirovecii in clinical<br />
samples<br />
Source : Journal <strong>of</strong> Clinical Microbiology, Volume 48, Issue 9, September <strong>2010</strong>, Pages 3165-3168<br />
Document Type : Article<br />
Keywords : large subunit ribosomal RNA; 3' untranslated region, 5' untranslated region, article, bacterium culture, bacterium<br />
detection, clinical evaluation, clinical trial, controlled clinical trial, controlled study, DNA extraction, human, lung<br />
lavage, major clinical study, microbial sensitivity test, mixed infection, multiplex polymerase chain reaction, Mycobacterium<br />
tuberculosis, nonhuman, Pneumocystis jiroveci, priority journal, sensitivity and specificity, single blind procedure; Clinical<br />
Laboratory Techniques, DNA Transposable Elements, DNA, Bacterial, DNA, Fungal, DNA, Mitochondrial, DNA,<br />
Ribosomal, Humans, Molecular Diagnostic Techniques, Mycobacterium tuberculosis, Pneumocystis Infections, Pneumocystis<br />
jirovecii, Polymerase Chain Reaction, Sensitivity and Specificity, Tuberculosis; Mycobacterium tuberculosis, Pneumocystis<br />
jirovecii<br />
42
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : A multiplex PCR assay for the simultaneous detection <strong>of</strong> Mycobacterium tuberculosis and Pneumocystis jirovecii was<br />
developed using IS6110-based detection for M. tuberculosis and mitochondrial large-subunit (mtLSU) rRNA gene detection for<br />
P. jirovecii. Ninety-five pulmonary blinded samples were examined using the developed multiplex PCR assay, and the results<br />
were compared with those obtained by the single nested PCRs targeting IS6110 for M. tuberculosis and mtLSU rRNA for P.<br />
jirovecii. Of the 95 pulmonary samples tested, the multiplex nested PCR developed here could detect 36 cases <strong>of</strong> M.<br />
tuberculosis infection, 35 cases <strong>of</strong> P. jirovecii infection, and 17 cases <strong>of</strong> M. tuberculosis and P. jirovecii coinfections. The<br />
sensitivities <strong>of</strong> the multiplex nested PCR in detecting M. tuberculosis and P. jirovecii were 92.1% and 81.4%, respectively,<br />
whereas the specificities in detecting M. tuberculosis and P. jirovecii were 98.2% and 100%, respectively. Copyright © <strong>2010</strong>,<br />
American Society for Microbiology. All Rights Reserved.<br />
No.109<br />
Author (s) : Boonkorkuea, N., Lenbury, Y., Alvarado, F.J., Wollkind, D.J.<br />
Title : Nonlinear stability analyses <strong>of</strong> vegetative pattern formation in an arid environment<br />
Source : Journal <strong>of</strong> Biological Dynamics, Volume 4, Issue 4, July <strong>2010</strong>, Pages 346-380<br />
Document Type : Article<br />
Keywords : Pattern formation; Vegetation distribution; Weakly nonlinear analysis<br />
<strong>Abstract</strong> : The development <strong>of</strong> spontaneous stationary vegetative patterns in an arid isotropic homogeneous environment is<br />
investigated by means <strong>of</strong> various weakly nonlinear stability analyses applied to the appropriate governing equation for this<br />
phenomenon. In particular, that process can be represented by a fourth-order partial differential time-evolution logistic<br />
equation for the total plant biomass per unit area divided by the carrying capacity <strong>of</strong> its territory and defined on an<br />
unbounded flat spatial domain. Those patterns that consist <strong>of</strong> parallel stripes, labyrinth-like mazes, rhombic arrays <strong>of</strong><br />
rectangular patches, and hexagonal distributions <strong>of</strong> spots or gaps are generated by the balance between the effects <strong>of</strong> shortrange<br />
facilitation and long-range competition. Then those theoretical predictions are compared with both relevant<br />
observational evidence and existing numerical simulations as well as placed in the context <strong>of</strong> the results from some recent<br />
nonlinear pattern formation studies. © <strong>2010</strong> Taylor & Francis.<br />
No.110<br />
Author (s) : Boonkusol, D., Dinnyes, A., Faisaikarm, T., Sangsuwan, P., Pratipnatalang, N., Sa-Ardrit, M., Saikhun, K., Svasti, S., Vadolas, J.,<br />
Winichagoon, P., Fucharoen, S., Kitiyanant, Y.<br />
Title : Effect <strong>of</strong> human β-globin bacterial artificial chromosome transgenesis on embryo cryopreservation in mouse models<br />
Source : Reproduction, Fertility and Development, Volume 22, Issue 5, <strong>2010</strong>, Pages 788-795<br />
Document Type : Article<br />
Keywords : Embryo banking; Thalassaemia transgenic<br />
<strong>Abstract</strong> : The purpose <strong>of</strong> the present study was to investigate the efficiency <strong>of</strong> embryo cryopreservation for four transgenic (TG)<br />
thalassaemic mouse strains, which is a key element <strong>of</strong> the ongoing gene banking efforts for these highvalue animals.<br />
Heterozygous TG embryos were produced by breeding four lines <strong>of</strong> TG males to wild-type (WT) females (C57BL/6J). Intact<br />
two-cell embryos were cryopreserved by vitrification in straws using 35% ethylene glycol. Survival rates <strong>of</strong> cryopreserved<br />
embryos ranged between 91.1% (102/112) and 93.6% (176/188) without significant differences between the lines. In contrast,<br />
the paternal line had a significant effect on the development <strong>of</strong> these embryos to the blastocyst stage, which ranged from<br />
50.6% (92/182) to 77.5% (79/102). This effect was also noted following embryo transfers, with implantation rates varying from<br />
17.3% (19/110) to 78.1% (35/45). The results demonstrate that the in vivo developmental potential is significantly influenced<br />
byTG line and reveal a specific line effect on cryosurvival. All bacterial artificial chromosome transgenic fetuses developed<br />
from vitrified-warmed embryos showed expression <strong>of</strong> the human β-globin transgene. In conclusion, the present study shows<br />
a strongTG line effect on developmental competence following cryopreservation and the vitrification method was successful<br />
to bank the human β-globin TG-expressing mouse strains. © <strong>2010</strong> CSIRO.<br />
No.111<br />
Author (s) : Boonkusol, D., Saikhun, K., Ratanaphumma, P.<br />
Title : Effects <strong>of</strong> extender and storage time on motility and ultrastructure <strong>of</strong> cooled-preserved boar spermatozoa<br />
Source : Kasetsart Journal - Natural Science, Volume 44, Issue 4, July <strong>2010</strong>, Pages 582-589<br />
Document Type : Review<br />
Keywords : Boar spermatozoa; Extender; SEM; TEM; Ultrastructure<br />
43
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : Artificial insemination (AI) using fresh diluted semen is used worldwide in the porcine industry. Viability <strong>of</strong> spermatozoa in<br />
diluted semen depends on several factors, such as the interaction with the type <strong>of</strong> extender and storage duration. The aim <strong>of</strong><br />
this study was to evaluate the effects <strong>of</strong> extenders and storage time on the motility and ultrastructure <strong>of</strong> cooled-preserved<br />
boar spermatozoa. Semen samples were collected, diluted in BTS, Merck III or Androhep and stored at 15°C for 0, 1, 3, 5 and<br />
7 d. The samples were warmed (37°C) and spermatozoa were evaluated for motility using light microscopy and ultrastructure<br />
using scanning (SEM) and transmission (TEM) electron microscopy. The results showed that motility did not differ significantly<br />
(P>0.05) among extenders at days 0 to 3, while Androhep showed a significantly (P
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.114<br />
Author (s) : Boonsarngsuk, V., Raweelert, P., Sukprapruet, A., Chaiprasithikul, R., Kiatboonsri, S.<br />
Title : Factors affecting the diagnostic yield <strong>of</strong> flexible bronchoscopy without guidance in pulmonary nodules or masses<br />
Source : Singapore Medical Journal, Volume 51, Issue 8, August <strong>2010</strong>, Pages 660-665<br />
Document Type : Article<br />
Keywords : Diagnostic yield; Flexible bronchoscopy; Guidance; Pulmonary nodule<br />
<strong>Abstract</strong> : Introduction: In day-to-day bronchoscopic practice, no guidance is available to pinpoint the precise location <strong>of</strong> pulmonary<br />
nodules or masses, especially in developing countries. This results in a large number <strong>of</strong> non-diagnostic testings. The present<br />
study aimed to determine the predictors <strong>of</strong> diagnostic yield in bronchoscopy without guidance and develop a model to<br />
predict the decision to perform this procedure. Methods: A retrospective study was conducted on 330 patients with<br />
pulmonary nodules or masses without any sign <strong>of</strong> atelectasis on chest radiographs, who underwent diagnostic bronchoscopy<br />
without guidance between June 2004 and May 2008. The patient characteristics, as well as radiological and bronchoscopic<br />
fndings were included in the analysis <strong>of</strong> factors affecting the diagnostic yield. Results: The overall diagnostic yield <strong>of</strong><br />
bronchoscopy was 55.8 percent. The tumour size, endobronchial visibility and the characteristics <strong>of</strong> endobronchial<br />
abnormalities were predictors <strong>of</strong> higher diagnostic yield. The prediction model was developed from the data that can be<br />
recognised before bronchoscopy. Bronchoscopy provided the diagnosis in 66.4 percent <strong>of</strong> the patients who had a tumour size<br />
<strong>of</strong> 4 cm or larger. Conclusion: The diagnostic yield <strong>of</strong> bronchoscopy without guidance was infuenced by the size <strong>of</strong> the lesion,<br />
the endobronchial visibility and the characteristics <strong>of</strong> endobronchial abnormalities. Computed tomography (CT) <strong>of</strong> the chest<br />
should be performed to evaluate airway involvement. If the lesion is less than 4 cm in diameter and there is a negative CT<br />
illustration <strong>of</strong> airway involvement, flexible bronchoscopy with guidance should be considered.<br />
No.115<br />
Author (s) : Boonsnongcheep, P., Korsangruang, S., Soonthornchareonnon, N., Chintapakorn, Y., Saralamp, P., Prathanturarug, S.<br />
Title : Growth and is<strong>of</strong>lavonoid accumulation <strong>of</strong> Pueraria candollei var. candollei and P. candollei var. mirifica cell suspension<br />
cultures<br />
Source : Plant Cell, Tissue and Organ Culture, Volume 101, Issue 2, May <strong>2010</strong>, Pages 119-126<br />
Document Type : Article<br />
Keywords : Analytical method validation; Cell suspension culture; HPLC; Is<strong>of</strong>lavonoids; Pueraria candollei<br />
<strong>Abstract</strong> : We established cell suspension cultures derived from leaf, stem, and root calli <strong>of</strong> Pueraria candollei var. candollei and P.<br />
candollei var. mirifica using liquid Murashige and Skoog (MS) medium supplemented with 0.56 μM 6-benzyladenine (BA) and<br />
4.52 μM 2,4-dichlorophenoxyacetic acid (2,4-D). Growth <strong>of</strong> the cell suspension cultures progressed to the stationary phase<br />
within 15-24 days. Methanolic extracts <strong>of</strong> cell suspension cultures <strong>of</strong> both varieties <strong>of</strong> P. candollei were analyzed using a<br />
validated HPLC protocol. All cell lines derived from leaf, stem, and root explants produced four major is<strong>of</strong>lavonoids: daidzein,<br />
daidzin, genistein, and genistin; these is<strong>of</strong>lavonoids were detected only in the roots <strong>of</strong> intact plants. Furthermore, the<br />
is<strong>of</strong>lavonoid contents <strong>of</strong> the cell suspension cultures were higher than those <strong>of</strong> intact plants. Thus, cell suspension culture <strong>of</strong><br />
both varieties <strong>of</strong> P. candollei may be an effective tool for is<strong>of</strong>lavonoid production. © <strong>2010</strong> Springer Science+Business Media<br />
B.V.<br />
No.116<br />
Author (s) : Boontanom, P., Siripattanapipong, S., Mungthin, M., Tan-Ariya, P., Leelayoova, S.<br />
Title : Improved sensitivity <strong>of</strong> PCR amplification <strong>of</strong> glutamate dehydrogenase gene for detection and genotyping <strong>of</strong> Giardia<br />
duodenalis in stool specimen<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 2, March <strong>2010</strong>, Pages 280-284<br />
Document Type : Article<br />
Keywords : Giardia duodenalis; Glutamate dehydrogenase gene; PCR; Sensitivity<br />
<strong>Abstract</strong> : A modified set <strong>of</strong> primers was developed to increase the sensitivity <strong>of</strong> nested PCR amplification <strong>of</strong> glutamate dehydrogenase<br />
(gdh) gene to detect and genotype Giardia duodenalis cysts in stool specimens. This modified set <strong>of</strong> primers had a<br />
significantly higher sensitivity (82%) than that <strong>of</strong> a previously published PCR primer set (53%).<br />
No.117<br />
Author (s) : Boontanon, N., Watanabe, S., Odate, T., Yoshida, N.<br />
45
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Methane production, consumption and its carbon isotope ratios in the Southern Ocean during the austral summer<br />
Source : Biogeosciences Discussions, Volume 7, Issue 5, <strong>2010</strong>, Pages 7207-7225<br />
Document Type : Article<br />
Keywords : air-sea interaction, carbon isotope, chlorophyll, isotopic ratio, methane, oxidation, plankton, supersaturation, vertical<br />
distribution; Southern Ocean<br />
<strong>Abstract</strong> : The distribution <strong>of</strong> dissolved CH4 in the Southern Ocean at 140° E was measured during the austral summer. Surface CH4 was<br />
supersaturated on average, and the calculated mean sea-air flux rate was 0.32 μmol m-2 d-1. The vertical distributions<br />
exhibited a CH4 maximum at approximately 125 m (∆CH4, 2.94 nM) below the chlorophyll-rich layer, suggesting a<br />
relationship between CH4 production and plankton dynamics in this area. CH4 oxidation and ocean movement characteristics<br />
in the deep layer led to the enrichment and fluctuation <strong>of</strong> δ13CCH4. We estimated the influence <strong>of</strong> Southern Ocean CH4, a<br />
source <strong>of</strong> isotopically heavy CH4 to the atmosphere, on the global CH4 budget to be approximately 0.19 Gg d-1. © <strong>2010</strong><br />
Author(s).<br />
No.118<br />
Author (s) : Boontanon, N., Watanabe, S., Odate, T., Yoshida, N.<br />
Title : Production and consumption mechanisms <strong>of</strong> N2O in the Southern Ocean revealed from its isotopomer ratios<br />
Source : Biogeosciences Discussions, Volume 7, Issue 5, <strong>2010</strong>, Pages 7821-7848<br />
Document Type : Review<br />
Keywords : cold water, deep water, denitrification, isotopic ratio, nitrous oxide, research work, vertical distribution, vertical movement;<br />
Antarctica, Southern Ocean<br />
<strong>Abstract</strong> : The distribution <strong>of</strong> dissolved N2O in the Southern Ocean at 140° E was measured during the austral summer (February-March<br />
2002) in the framework <strong>of</strong> the 43rd Japanese Antarctic Research Expedition (JARE-43). Surface-dissolved N2O was<br />
undersaturated (about 94% saturation), and the calculated mean sea-air flux rate was -3.68±2.57 μmol m -2 d-1. The vertical<br />
distributions tested exhibited N 2O maxima at around 150-300 m (∆N2O, 7.90-8.51 nM) below the chlorophyll-rich layer,<br />
which coincided with the layer <strong>of</strong> minimum oxygen. These observations strongly suggest that N2O production and<br />
consumption are related to apparent oxygen utilization (AOU). In the deeper layer, the presence <strong>of</strong> anoxic microsites within<br />
particles, together with the horizontal and vertical movement <strong>of</strong> cold water around Antarctica, is one <strong>of</strong> the parameters that<br />
govern the intramolecular distribution <strong>of</strong> the isotopic composition <strong>of</strong> N2O. The N2O isotopic compositions in the maximum<br />
layer were +7.3 to +8.2‰° for δ15Nbulk and +43.5 to +46.2‰° for δ18O associated with the coupling <strong>of</strong> nitrification and<br />
denitrification production mechanisms. Site preference decreased from an average 17‰° at the surface to the ∆N2O<br />
maximum and slightly increased with depth up to 24‰° at the deeper region. The influence <strong>of</strong> deep Southern Ocean N2O on<br />
the global N2O budget is estimated to be about 46.2±5.3 Mg N2O-N d -1, which represents the amount that can escape to<br />
the atmosphere and thus contribute to emissions into the world's oceans.<br />
No.119<br />
Author (s) : Boontem, P.; Govitrapong, P.<br />
Title : EFFECTS OF AMPHETAMINE ON DOPAMINERGIC NEUROTRANSMISSION IN NEONATAL RAT STRIATUM<br />
Source : JOURNAL OF NEUROCHEMISTRY, Volume: 115, Supplement: 1, Pages: 84-84, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.120<br />
Author (s) : Boonyapisit, W., Tresukosol, D.<br />
Title : Comparison <strong>of</strong> the incidence <strong>of</strong> imidapril and enalapril induced cough.<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand = Chotmaihet thangphaet, Volume 93 Suppl 1, January <strong>2010</strong>, Pages S48-53<br />
Document Type : Article<br />
Keywords : dipeptidyl carboxypeptidase inhibitor, enalapril, imidapril, imidazolidine derivative; aged, article, chemically induced<br />
disorder, comparative study, coughing, crossover procedure, female, heart left ventricle<br />
function, human, hypertension, incidence, male, middle aged, oral drug administration, treatment outcome; Administration,<br />
Oral, Aged, Angiotensin-Converting Enzyme Inhibitors, Cough, Cross-Over<br />
46
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Studies, Enalapril, Female, Humans, Hypertension, Imidazolidines, Incidence, Male, Middle Aged, Treatment<br />
Outcome, Ventricular Dysfunction, Left<br />
<strong>Abstract</strong> : BACKGROUND: Imidapril is an angiotensin converting enzyme (ACE) inhibitor without a sulfhydril group which has been shown<br />
from previous study to have low incidence <strong>of</strong> ACE inhibitor induced cough. OBJECTIVE: To compare the incidence <strong>of</strong> cough<br />
between two ACE inhibitors, imidapril and enalapril. MATERIAL AND METHOD: A comparative cross over study was performed<br />
in 119 patients with hypertension or left ventricular dysfunction. Patients were assigned to one <strong>of</strong> the two treatment groups,<br />
either a group receiving imidapril or enalapril for 4 weeks (Period I) and then these same groups were crossed over to receive<br />
either enalapril or imidapril for 4 weeks (Period II). The occurrence <strong>of</strong> cough during treatment was monitored by interviewing<br />
the patients. RESULTS: The incidence <strong>of</strong> cough was 44 % while on imidapril treatment and 66% while on enalapril treatment<br />
(p = 0.0014). The antihypertensive effects <strong>of</strong> two drugs were not different. CONCLUSION: The incidence <strong>of</strong> cough was<br />
significantly less under imidapril than under enalapril treatment, while there was no difference in the antihypertensive effects<br />
between the two ACE inhibitors.<br />
No.121<br />
Author (s) : Boonyasiranant, T., Flamm, S.D.<br />
Title : Delayed-enhancement cardiac MRI in the evaluation <strong>of</strong> cardiomyopathies<br />
Source : Imaging in Medicine, 2 (3), pp. 289-302.<br />
Document Type : Review<br />
Keywords : cardiomyopathy; infiltrative disorders; ischemic heart disease; MRI; noninvasive imaging<br />
<strong>Abstract</strong> : Cardiomyopathies are a diverse group <strong>of</strong> disorders with etiologies ranging from ischemic to infiltrative. Clinical differentiation<br />
may be complex, but diagnosis is essential as complications include congestive heart failure and sudden death. Recent<br />
developments in cardiac MRI, particularly the implementation <strong>of</strong> delayed-enhancement imaging has introduced a novel<br />
approach to noninvasive tissue characterization that can more accurately differentiate the spectrum <strong>of</strong> cardiomyopathies. This<br />
article summarizes the comprehensive diagnostic abilities <strong>of</strong> cardiac MRI in defining cardiac anatomy and physiology and,<br />
more importantly, identifying pathologic tissues and differentiating those with substrates amenable to therapy. The value <strong>of</strong><br />
delayed-enhancement imaging in prognostics will also be presented. © <strong>2010</strong> Future Medicine Ltd.<br />
No.122<br />
Author (s) : Boonyopakorn, N., Sripongpun, N., Thanachayanont, C., Dangtip, S.<br />
Title : Effects <strong>of</strong> substrate temperature and vacuum annealing on properties <strong>of</strong> ito films prepared by radio-frquency magnetron<br />
sputtering<br />
Source : Chinese Physics Letters, Volume 27, Issue 10, October <strong>2010</strong>, Article number 108103<br />
Document Type : Article<br />
<strong>Abstract</strong> : Indium tin oxide (ITO) films were prepared by rf magnetron sputtering under two conditions: (i) at substrate temperature Ta<br />
from room temperature (RT) to 350°C, (ii) with additional post-annealing in vacuum at 400°C for 30 min in comparison <strong>of</strong> their<br />
crystalline structures, and electrical-optical properties <strong>of</strong> the films deposited. From the experimental results, it is found that,<br />
under the first condition, the crystalline structures and the electrical-optical properties <strong>of</strong> the films are improved with the<br />
increasing Ta. Under the other condition, i.e. with the additional post-annealing, the films exhibit higher degree <strong>of</strong><br />
crystallinities and better electrical-optical properties. Under the two deposition conditions, inter-relation between electricaloptical<br />
properties and the crystalline structure is observed clearly. However, even under the same annealing condition, it is<br />
observed that improved properties <strong>of</strong> the films are different, depending on their deposition temperatures, which implies that<br />
an initial stage <strong>of</strong> the ITO film before annealing is an important factor for the film's properties improved after annealing. The<br />
resistivity <strong>of</strong> 2.33 × 10-4ωcm can be achieved at Ta <strong>of</strong> 350°C after annealing. © <strong>2010</strong> Chinese Physical Society and IOP<br />
Publishing Ltd.<br />
No.123<br />
Author (s) : Boonyos, P., Soonsanga, S., Boonserm, P., Promdonkoy, B.<br />
Title : Role <strong>of</strong> cysteine at positions 67, 161 and 241 <strong>of</strong> a Bacillus sphaericus binary toxin BinB<br />
Source : BMB Reports, Volume 43, Issue 1, January <strong>2010</strong>, Pages 23-28<br />
Document Type : Article<br />
Keywords : Bacillus sphaericus; Binary toxin; Disulfide bond; Mosquito-larvicidal toxin; Mutagenesis<br />
47
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : Binary toxin consisting <strong>of</strong> BinA and BinB from Bacillus sphaericus is toxic to mosquito larvae. BinB is responsible for specific<br />
binding to the larval gut cell membrane while BinA is crucial for toxicity. To investigate functional role <strong>of</strong> cysteine in BinB,<br />
three cysteine residues at positions 67, 161, and 241 were replaced by alanine or serine. Mutations at these positions did not<br />
affect protein production and overall structure <strong>of</strong> BinB. These cysteine residues are not involved in disulfide bond formation<br />
between BinB molecules. Mosquito-larvicidal assays revealed that C67 and C161 are essential for toxicity, whereas C241 is<br />
not. Mutations at C67 and C161 resulted in weaker BinA-BinB interaction. The loss <strong>of</strong> toxicity may be due to the reduction <strong>of</strong><br />
interactions between BinA and BinB or BinB and its receptor. C67 and C161 could also play a part during conformational<br />
changes or internalization <strong>of</strong> the binary toxin into the target cell.<br />
No.124<br />
Author (s) : Bouyer, J., Ravel, S., Guerrini, L., Dujardin, J.-P., Sidibé, I., Vreysen, M.J.B., Solano, P., De Meeûs, T.<br />
Title : Population structure <strong>of</strong> Glossina palpalis gambiensis (Diptera: Glossinidae) between river basins in Burkina Faso: Consequences<br />
for area-wide integrated pest management<br />
Source : Infection, Genetics and Evolution, Volume 10, Issue 2, March <strong>2010</strong>, Pages 321-328<br />
Document Type : Article<br />
Keywords : Area-wide integrated pest management; Gene flow; River basins; Trypanosomosis; Tsetse<br />
<strong>Abstract</strong> : African animal trypanosomosis is a major obstacle to the development <strong>of</strong> more efficient and sustainable livestock production<br />
systems in West Africa. Riverine tsetse species such as Glossina palpalis gambiensis Vanderplank are their major vectors. A<br />
wide variety <strong>of</strong> control tactics is available to manage these vectors, but their elimination will only be sustainable if control is<br />
exercised following area-wide integrated pest management (AW-IPM) principles, i.e. the control effort is targeting an entire<br />
tsetse population within a circumscribed area. In the present study, genetic variation at microsatellite DNA loci was used to<br />
examine the population structure <strong>of</strong> G. p. gambiensis inhabiting two adjacent river basins, i.e. the Comoé and the Mouhoun<br />
River basins in Burkina Faso. A remote sensing analysis revealed that the woodland savannah habitats between the river<br />
basins have remained unchanged during the last two decades. In addition, genetic variation was studied in two populations<br />
that were separated by a man-made lake originating from a dam built in 1991 on the Comoé. Low genetic differentiation was<br />
observed between the samples from the Mouhoun and the Comoé River basins and no differentiation was found between<br />
the samples separated by the dam. The data presented indicate that the overall genetic differentiation <strong>of</strong> G. p. gambiensis<br />
populations inhabiting two adjacent river basins in Burkina Faso is low (FST = 0.016). The results <strong>of</strong> this study suggest that<br />
either G. p. gambiensis populations from the Mouhoun are not isolated from those <strong>of</strong> the Comoé, or that the isolation is too<br />
recent to be detected. If elimination <strong>of</strong> the G. p. gambiensis population from the Mouhoun River basin is the selected control<br />
strategy, re-invasion from adjacent river basins may need to be prevented by establishing a buffer zone between the<br />
Mouhoun and the other river basin(s). © <strong>2010</strong> Elsevier B.V. All rights reserved.<br />
No.125<br />
Author (s) : Bovonsombat, P., Ali, R., Khan, C., Leykajarakul, J., Pla-On, K., Aphimanchindakul, S., Pungcharoenpong, N., Timsuea, N.,<br />
Arunrat, A., Punpongjareorn, N.<br />
Title : Facile p-toluenesulfonic acid-promoted para-selective monobromination and chlorination <strong>of</strong> phenol and analogues<br />
Source : Tetrahedron, Volume 66, Issue 34, 21 August <strong>2010</strong>, Pages 6928-6935<br />
Document Type : Article<br />
Keywords : n bromosuccinimide, n chlorosuccinimide, phenol, succinimide, sulfonic acid derivative, toluenesulfonic acid, unclassified<br />
drug; article, bromination, carbon nuclear magnetic resonance, catalysis, chlorination, gas chromatography, mass<br />
spectrometry, priority journal, proton nuclear magnetic resonance, room temperature<br />
<strong>Abstract</strong> : para-Regioselective bromination <strong>of</strong> phenol and analogues, promoted by p-toluenesulfonic acid, is achieved in high to<br />
excellent yields at room temperature with N-bromosuccinimide. Chlorination with N-chlorosuccinimide and catalysed by ptoluenesulfonic<br />
acid also gives para-chlorinated phenol analogues in good yields at room temperature. para-Bromination <strong>of</strong><br />
phenol, promoted by p-toluenesulfonic acid, is achieved in excellent yields at room temperature with N-bromosuccinimide.<br />
p-Toluenesulfonic acid is also effective as a promoter <strong>of</strong> para-chlorination with N-chlorosuccinimide. © <strong>2010</strong> Elsevier Ltd. All<br />
rights reserved.<br />
No.126<br />
Author (s) : Bovornratanaraks, T., Kotmool, K., Yoodee, K., McMahon, M.I., Ruffolo, D.<br />
Title : High pressure structural studies <strong>of</strong> AgInTe2<br />
48
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Source : Journal <strong>of</strong> Physics: Conference Series, Volume 215, <strong>2010</strong>, Article number 012008<br />
Document Type : Conference Paper<br />
Keywords : Ambient pressures, Chalcopyrite semiconductor, Chalcopyrite structures, Coexisting phasis, High pressure, Image<br />
plates, Orthorhombic structures, Pressure increase, Space Groups, Structural phase transformations, Structural studies, Zincblende;<br />
Holographic interferometry, Phase transitions, X ray diffraction; Copper compounds<br />
<strong>Abstract</strong> : The structural phase transformations in the chalcopyrite semiconductor AgInTe2 have been studied up to 10 GPa on both<br />
pressure increase and decrease. The experiments were conducted using angle-dispersive X-ray diffraction with synchrotron<br />
radiation and an image plate. The diffraction patterns <strong>of</strong> AgInTe2 at ambient pressure reveal two coexisting phases: the first<br />
has the chalcopyrite structure while the second has a zincblende-like structure. On pressure increase both phases<br />
transformed at 3-4 GPa to a cation-disordered orthorhombic structure with spacegroup Cmcm. On pressure decrease, the<br />
chalcopyrite phase started to reappear at 0.55 GPa, and the Cmcm phase disappeared completely at ambient pressure. ©<br />
<strong>2010</strong> IOP Publishing Ltd.<br />
No.127<br />
Author (s) : Bovornratanaraks, T., Saengsuwan, V., Yoodee, K., McMahon, M.I., Hejny, C., Ruffolo, D.<br />
Title : High pressure orthorhombic structure <strong>of</strong> CuInSe2<br />
Source : Journal <strong>of</strong> Physics Condensed Matter, Volume 22, Issue 35, 8 September <strong>2010</strong>, Article number 355801<br />
Document Type : Article<br />
Keywords : Ambient pressures, Cubic structure, CuInSe2, High pressure, High-pressure phase, High-pressure structures, Lattice<br />
parameters, Orthorhombic structures, Space Groups, Structural behaviour, Structural phase transition, Structural<br />
studies, Tetragonal structure, Type structures; Copper compounds, Sodium chloride, X ray powder diffraction; Phase<br />
transitions<br />
<strong>Abstract</strong> : The structural behaviour <strong>of</strong> CuInSe2 under high pressure has been studied up to 53 GPa using angle-dispersive x-ray powder<br />
diffraction techniques. The previously reported structural phase transition from its ambient pressure tetragonal structure to a<br />
high pressure phase with a NaCl-like cubic structure at 7.6 GPa has been confirmed. On further compression, another<br />
structural phase transition is observed at 39 GPa. A full structural study <strong>of</strong> this high pressure phase has been carried out and<br />
the high pressure structure has been identified as orthorhombie with space group Cmcm and lattice parameters a = 4.867(8)<br />
Å, b = 5.023(8) Å and c = 4.980(3) Å at 53.2(2) GPa. This phase transition behaviour is similar to those <strong>of</strong> analogous binary and<br />
trinary semiconductors, where the orthorhombie Cmcm structure can also be viewed as a distortion <strong>of</strong> the cubic NaCl-type<br />
structure. © <strong>2010</strong>IOP Publishing Ltd.<br />
No.128<br />
Author (s) : Bowden, C.L., Mosolov, S., Hranov, L., Chen, E., Habil, H., Kongsakon, R., Manfredi, R., Lin, H.N.<br />
Title : Efficacy <strong>of</strong> valproate versus lithium in mania or mixed mania: a randomized, open 12-week trial<br />
Source : INTERNATIONAL CLINICAL PSYCHOPHARMACOLOGY, Volume: 25, Issue: 2, Pages: 60-67, DOI: 10.1097/YIC.0b013e328333ac1b,<br />
Published: MAR <strong>2010</strong><br />
Document Type : Article<br />
Keywords : acute mania; bipolar disorder; Eastern Europe; lithium; South East Asia; valproate<br />
<strong>Abstract</strong> : The objective <strong>of</strong> this study was to compare the efficacy and safety <strong>of</strong> valproate and lithium in bipolar I patients experiencing<br />
a manic or a mixed episode. This international, randomized, open-label, parallel-group, equivalence study included 268<br />
patients with bipolar I disorder. The starting dose <strong>of</strong> valproate was 20 mg/kg/day and that <strong>of</strong> lithium was 800 mg/day.<br />
Treatment duration was 12 weeks. The primary outcome measure was mean change in Young Mania Rating Scale score<br />
between baseline and study end. Secondary outcome measures were response and remission rates, change in Montgomery<br />
and Asberg Depression Rating Scale and Clinical Global Impression Bipolar Disorder instrument score, and occurrence <strong>of</strong><br />
adverse events. The mean change from baseline in Young Mania Rating Scale score was 15.8 +/- 5.3 in the lithium group and<br />
17.3 +/- 9.4 in the valproate group. The 90% confidence interval <strong>of</strong> the intergroup difference (-0.69; 3.31) was within<br />
prespecified equivalence limits. Response rates were 72.6% in the lithium group and 79.5% in the valproate group. Remission<br />
rates were 58.5 and 71.9%, respectively. No intergroup differences were observed in median time to treatment response (21<br />
days) or change in Clinical Global Impression Bipolar Disorder instrument or Montgomery and Asberg Depression Rating Scale<br />
scores. Adverse events were reported in 42.8% <strong>of</strong> patients in the lithium group and 41.5% in the valproate group. Valproate<br />
and lithium showed comparable efficacy and tolerability in the treatment <strong>of</strong> acute mania over 12 weeks. Int Clin<br />
Psychopharmacol 25: 60-67 (C) <strong>2010</strong> Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.<br />
49
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.129<br />
Author (s) : Bowers, J.R., Engelthaler, D.M., Ginther, J.L., Pearson, T., Peacock, S.J., Tuanyok, A., Wagner, D.M., Currie, B.J., Keim, P.S.<br />
Title : BurkDiff: A real-time PCR allelic discrimination assay for burkholderia Pseudomallei and B. mallei<br />
Source : PLoS ONE, Volume 5, Issue 11, <strong>2010</strong>, Article number e15413<br />
Document Type : Article<br />
Keywords : allele, article, bacterium detection, bacterium isolate, BurkDiff, Burkholderia mallei, Burkholderia pseudomallei, controlled<br />
study, genetic screening, molecular probe, nonhuman, real time polymerase chain reaction, sensitivity and specificity, single<br />
nucleotide polymorphism, animal, bacterial genome, bacterium identification, classification, differential<br />
diagnosis, genetics, glanders, human, isolation and purification, melioidosis, methodology, microbiology, polymerase chain<br />
reaction, reproducibility; Burkholderia pseudomallei; bacterial DNA, bacterial protein; Alleles, Animals, Bacterial<br />
Proteins, Bacterial Typing Techniques, Burkholderia mallei, Burkholderia pseudomallei, Diagnosis, Differential, DNA,<br />
Bacterial, Genome, Bacterial, Glanders, Humans, Melioidosis, Polymerase Chain Reaction, Polymorphism, Single<br />
Nucleotide, Reproducibility <strong>of</strong> Results, Sensitivity and Specificity<br />
<strong>Abstract</strong> : A real-time PCR assay, BurkDiff, was designed to target a unique conserved region in the B. pseudomallei and B. mallei<br />
genomes containing a SNP that differentiates the two species. Sensitivity and specificity were assessed by screening BurkDiff<br />
across 469 isolates <strong>of</strong> B. pseudomallei, 49 isolates <strong>of</strong> B. mallei, and 390 isolates <strong>of</strong> clinically relevant non-target species.<br />
Concordance <strong>of</strong> results with traditional speciation methods and no cross-reactivity to non-target species show BurkDiff is a<br />
robust, highly validated assay for the detection and differentiation <strong>of</strong> B. pseudomallei and B. mallei. © <strong>2010</strong> Bowers et al.<br />
No.130<br />
Author (s) : Brouwer, A.E., Teparrukkul, P., Rajanuwong, A., Chierakul, W., Mahavanakul, W., Chantratita, W., White, N.J., Harrison, T.S.<br />
Title : Cerebrospinal fluid HIV-1 viral load during treatment <strong>of</strong> cryptococcal meningitis<br />
Source : Journal <strong>of</strong> Acquired Immune Deficiency Syndromes, Volume 53, Issue 5, April <strong>2010</strong>, Pages 668-669<br />
Document Type : Letter<br />
Keywords : CD4 antigen, gamma interferon, granulocyte colony stimulating factor, granulocyte macrophage colony stimulating<br />
factor, interleukin 10, interleukin 12p70, interleukin 13, interleukin 17, interleukin 1beta, interleukin 2, interleukin 4, interleukin<br />
5, interleukin 6, interleukin 7, interleukin 8, macrophage inflammatory protein 1beta, monocyte chemotactic protein 1, tumor<br />
necrosis factor alpha, virus RNA; bacterial meningitis, CD4 lymphocyte count, cerebrospinal fluid, Cryptococcus<br />
ne<strong>of</strong>ormans, human, Human immunodeficiency virus 1, Human immunodeficiency virus 1 infection, letter, lymphocyte<br />
activation, nonhuman, opportunistic infection, priority journal, virus inhibition, virus load; Cryptococcus ne<strong>of</strong>ormans, HIV<br />
Infections, HIV-1, Humans, Meningitis, Cryptococcal, RNA, Viral<br />
<strong>Abstract</strong> : –<br />
No.131<br />
Author (s) : Buates, S., Bantuchai, S., Sattabongkot, J., Han, E.-T., Tsuboi, T., Udomsangpetch, R., Sirichaisinthop, J., Tan-ariya, P.<br />
Title : Development <strong>of</strong> a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) for clinical detection <strong>of</strong><br />
Plasmodium falciparum gametocytes<br />
Source : Parasitology <strong>International</strong>, Volume 59, Issue 3, September <strong>2010</strong>, Pages 414-420<br />
Document Type : Article<br />
Keywords : Gametocyte detection; P. falciparum gametocytes; Pfs16; Pfs25; Reverse transcription-loop-mediated isothermal<br />
amplification (RT-LAMP)<br />
<strong>Abstract</strong> : Plasmodium falciparum gametocytes are usually present in peripheral blood at a very low level, thus requiring a sensitive<br />
assay detection method. In this study, reverse transcription-loop-mediated isothermal amplification (RT-LAMP) was developed<br />
for clinical detection <strong>of</strong> P. falciparum gametocytes. Transcripts <strong>of</strong> Pfs16 <strong>of</strong> sexually committed ring and Pfs25 <strong>of</strong> mature<br />
gametocytes were detected by RT-LAMP in 82 clinical blood samples using nested RT-PCR as a gold standard. RT-LAMP<br />
demonstrated a detection limit <strong>of</strong> 1 parasitized red blood cell (RBC)/500μl <strong>of</strong> blood for both Pfs16 and Pfs25 transcripts. For<br />
Pfs16 transcript, RT-LAMP detected all 30 samples positive by nested RT-PCR (100% sensitivity) and 1 in 52 samples negative<br />
by nested RT-PCR (98.1% specificity). For Pfs25 transcript, RT-LAMP detected all 15 samples positive by nested RT-PCR (100%<br />
sensitivity) and none <strong>of</strong> 67 samples negative by nested RT-PCR (100% specificity). Negative predictive value (NPV) and positive<br />
predictive value (PPV) <strong>of</strong> RT-LAMP for detection <strong>of</strong> Pfs16 transcript were 100% and 96.8%, respectively, and 100% for both<br />
when employing Pfs25 transcript. Detection rate <strong>of</strong> Pfs16 and Pfs25 transcripts by RT-LAMP in microscopically gametocytenegative<br />
samples was 91.7% and 29.2%, respectively. Compared with nested RT-PCR, RT-LAMP had a higher sensitivity but<br />
50
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
similar specificity, with the advantage <strong>of</strong> a shorter assay time. As RT-LAMP requires very basic instruments and the results can<br />
be obtained by visual inspection, this technique provides a simple and reliable tool for epidemiological studies <strong>of</strong> malaria<br />
transmission and in gametocyte-targeted control programmes. © <strong>2010</strong> Elsevier Ireland Ltd.<br />
No.132<br />
Author (s) : Buates, S.<br />
Title : DETECTION OF FALCIPARUM GAMETOCYTES USING A REVERSE TRANSCRIPTION-LOOP-MEDIATED ISOTHERMAL AMPLIFICATION<br />
(RT-LAMP)<br />
Source : AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, Volume: 83, Issue: 5, Supplement: S, Pages: 40-40, Meeting<br />
<strong>Abstract</strong>: 134, Published: NOV <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.133<br />
Author (s) : Buathong, S., Mungthin, M., Tan-ariya, P., Naaglor, T., Taamasri, P., Suwannahitatorn, P., Leelayoova, S.<br />
Title : PBS incubation, a simple technique to release miracidia <strong>of</strong> Opisthorchis-like eggs for DNA extraction<br />
Source : Experimental Parasitology, Volume 124, Issue 3, March <strong>2010</strong>, Pages 357-359<br />
Document Type : Article<br />
Keywords : DNA extraction; Miracidia; Opisthorchis; PBS incubation; PCR<br />
<strong>Abstract</strong> : This study describes a simple technique for releasing miracidia from Opisthorchis-like eggs before DNA extraction by keeping<br />
PBS washed specimens at 4°C for two days. Using PCR amplification, the sensitivity <strong>of</strong> the PBS incubation technique was as<br />
good as that obtained from freeze-thaw technique. Moreover, this low-cost technique is less laborious. © 2009 Elsevier Inc.<br />
No.134<br />
Author (s) : Buchanan, J., Mihaylova, B., Gray, A., White, N.<br />
Title : Cost-effectiveness <strong>of</strong> pre-referral antimalarial, antibacterial, and combined rectal formulations for severe febrile illness<br />
Source : PLoS ONE, Volume 5, Issue 12, <strong>2010</strong>, Article number e14446<br />
Document Type : Article<br />
Keywords : antiinfective agent, antimalarial agent; Africa, article, Asia, bacterial infection, cost effectiveness analysis, disease<br />
severity, fever, health care cost, incidence, malaria, mortality, patient referral, child, cost benefit analysis, health care<br />
delivery, human, metabolism, microbiology, preschool child, rectal drug administration; Bacteria (microorganisms);<br />
Administration, Rectal, Africa, Anti-Infective Agents, Antimalarials, Bacterial Infections, Child, Child, Preschool, Cost-Benefit<br />
Analysis, Fever, Health Care Costs, Health Services Accessibility, Humans, Malaria<br />
<strong>Abstract</strong> : Background: Malaria and bacterial infections account for most infectious disease deaths in developing countries. Prompt<br />
treatment saves lives, but rapid deterioration <strong>of</strong>ten prevents the use <strong>of</strong> oral therapies; delays in reaching health facilities<br />
providing parenteral interventions are common. Rapidly and reliably absorbed antimalarial/antibacterial rectal formulations<br />
used in the community could prevent deaths and disabilities. Rectal antimalarial treatments are currently available; rectal<br />
antibacterial treatments are yet to be developed. Assessment <strong>of</strong> the likely cost-effectiveness <strong>of</strong> these interventions will<br />
inform research priorities and implementation. Methods and Findings: The burden <strong>of</strong> malaria and bacterial infections<br />
worldwide and in Sub-Saharan and Southern Africa (SSA) and South and South-East Asia (SEA) was summarised using<br />
published data. The additional healthcare costs (US$) per death and per Disability Adjusted Life Year (DALY) avoided following<br />
pre-referral treatment <strong>of</strong> severe febrile illness with rectal antimalarials, antibacterials or combined antimalarial/antibacterials<br />
in populations at malaria risk in SSA/SEA were assessed. 46 million severe malaria and bacterial infections and 5 million<br />
deaths occur worldwide each year, mostly in SSA/ SEA. At annual delivery costs <strong>of</strong> $0.02/capita and 100% coverage, rectal<br />
antimalarials ($2 per dose) would avert 240,000 deaths in SSA and 7,000 deaths in SEA at $5 and $177 per DALY avoided,<br />
respectively; rectal antibacterials ($2 per dose) would avert 130,000 deaths in SSA and 27,000 deaths in SEA at $19 and $97<br />
per DALY avoided, respectively. Combined rectal formulations ($2.50 per dose) would avert 370,000 deaths in SSA and 33,000<br />
deaths in SEA at $8 and $79 per DALY avoided, respectively, and are a cost-effective alternative to rectal antimalarials or<br />
antibacterials alone. Conclusions: Antimalarial, antibacterial and combined rectal formulations are likely to be cost-effective<br />
interventions for severe febrile illness in the community. Attention should focus on developing effective rectal antibacterials<br />
and ensuring that these lifesaving treatments are used in a cost-effective manner. © <strong>2010</strong> Buchanan et al.<br />
51
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.135<br />
Author (s) : Buchillet, D.<br />
Title : The conception <strong>of</strong> warm diseases in chinese medicine [La conception des maladies de tiédeur dans la médecine chinoise]<br />
Source : Revue d'Anthropologie des Connaissances, Volume 4, Issue 1, 30 June <strong>2010</strong>, Pages 195-241<br />
Document Type : Article<br />
Keywords : China; Chinese medicine; Epidemic outbreaks; Febrile diseases; Warm diseases<br />
<strong>Abstract</strong> : Since at least two thousand <strong>of</strong> years, Chinese medicine recognizes the role <strong>of</strong> abnormal climatic and environmental factors in<br />
the appearance <strong>of</strong> febrile diseases and in the emergence <strong>of</strong> epidemic outbreaks. Whereas they were mainly attributed in the<br />
past to the invasion <strong>of</strong> the body by pathogenic Wind and Cold, these diseases are since the foundation under the Qing<br />
Dynasty (1644- 1911) <strong>of</strong> the School <strong>of</strong> Warm Diseases also attributed to pathogenic factors <strong>of</strong> a warm or hot quality, and in<br />
the case <strong>of</strong> epidemic diseases, to "Pestilential Qi" and "Epidemic Warm Toxins" which exist in the environment. This article<br />
recounts the evolution <strong>of</strong> Chinese medical ideas and practices related to exogenous febrile diseases. It will show the<br />
evolution <strong>of</strong> the concept <strong>of</strong> Warm Diseases during the centuries as well as the importance and role <strong>of</strong> the epidemic<br />
outbreaks in the revision <strong>of</strong> medical discourses and practices related to these diseases. It comprehends two parts: The fi rst<br />
part reviews the fundamental principles <strong>of</strong> Chinese medicine; the second one recounts the evolution <strong>of</strong> Chinese medical<br />
ideas on the process <strong>of</strong> development and evolution <strong>of</strong> exogenous febrile diseases.<br />
No.136<br />
Author (s) : Buchillet, D., de Lamballerie, X.<br />
Title : The 1932 Macau epidemic <strong>of</strong> cerebrospinal meningitis: A historical perspective and critical review <strong>of</strong> the data<br />
Source : Infection, Genetics and Evolution, Volume 10, Issue 7, October <strong>2010</strong>, Pages 896-902<br />
Document Type : Article<br />
Keywords : Cerebrospinal meningitis; Epidemiology; History <strong>of</strong> meningitis epidemics; Macau; South China<br />
<strong>Abstract</strong> : Since the first clinical description by Vieusseux (1805) <strong>of</strong> the epidemic form <strong>of</strong> meningitis known today as cerebrospinal<br />
meningitis, numerous epidemic outbreaks <strong>of</strong> the disease were reported globally during the nineteenth and early twentieth<br />
century. Historical medical data confirmed that clinical disease may occur either sporadically or in an epidemic form.<br />
Moreover, it may afflict children, young military recruits and/or populations living under crowded conditions. In 1932, an<br />
epidemic <strong>of</strong> meningitis occurred in Macau. The disease was sufficiently unusual to justify the publication <strong>of</strong> a special report<br />
by the Portuguese physician in charge <strong>of</strong> the control services <strong>of</strong> the epidemic. Here we present a critical review <strong>of</strong> the Macau<br />
epidemic data. © <strong>2010</strong> Elsevier B.V.<br />
No.137<br />
Author (s) : Buie, T., Campbell, D.B., Fuchs III, G.J., Furuta, G.T., Levy, J., Van De Water, J., Whitaker, A.H., Atkins, D., Bauman, M.L., Beaudet,<br />
A.L., Carr, E.G., Gershon, M.D., Hyman, S.L., Jirapinyo, P., Jyonouchi, H., Kooros, K., Kushak, R., Levitt, P., Levy, S.E., Lewis, J.D.,<br />
Murray, K.F., Natowicz, M.R., Sabra, A., Wershil, B.K., Weston, S.C., Zeltzer, L., Winter, H.<br />
Title : Evaluation, diagnosis, and treatment <strong>of</strong> gastrointestinal disorders in individuals with ASDs: A consensus report<br />
Source : Pediatrics, Volume 125, Issue SUPPL. 1, January <strong>2010</strong>, Pages S1-S18<br />
Document Type : Conference Paper<br />
Keywords : Abdominal discomfort; Autism spectrum disorder; Chronic diarrhea; Constipation; Gastrointestinal; Gluten-free casein-free<br />
diet; Problem behavior<br />
<strong>Abstract</strong> : Autism spectrum disorders (ASDs) are common and clinically heterogeneous neurodevelopmental disorders. Gastrointestinal<br />
disorders and associated symptoms are commonly reported in individuals with ASDs, but key issues such as the prevalence<br />
and best treatment <strong>of</strong> these conditions are incompletely understood. A central difficulty in recognizing and characterizing<br />
gastrointestinal dysfunction with ASDs is the communication difficulties experienced by many affected individuals. A<br />
multidisciplinary panel reviewed the medical literature with the aim <strong>of</strong> generating evidence-based recommendations for<br />
diagnostic evaluation and management <strong>of</strong> gastrointestinal problems in this patient population. The panel concluded that<br />
evidence-based recommendations are not yet available. The consensus expert opinion <strong>of</strong> the panel was that individuals with<br />
ASDs deserve the same thoroughness and standard <strong>of</strong> care in the diagnostic workup and treatment <strong>of</strong> gastrointestinal<br />
concerns as should occur for patients without ASDs. Care providers should be aware that problem behavior in patients with<br />
ASDs may be the primary or sole symptom <strong>of</strong> the underlying medical condition, including some gastrointestinal disorders. For<br />
these patients, integration <strong>of</strong> behavioral and medical care may be most beneficial. Priorities for future research are identified<br />
52
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
to advance our understanding and management <strong>of</strong> gastrointestinal disorders in persons with ASDs. Copyright © 2009 by the<br />
American Academy <strong>of</strong> Pediatrics.<br />
No.138<br />
Author (s) : Bumrungpert, A., Kalpravidh, R.W., Chuang, C.-C., Overman, A., Martinez, K., Kennedy, A., McIntosh, M.<br />
Title : Xanthones from mangosteen inhibit inflammation in human macrophages and in human adipocytes exposed to macrophageconditioned<br />
media<br />
Source : Journal <strong>of</strong> Nutrition, Volume 140, Issue 4, April <strong>2010</strong>, Pages 842-847<br />
Document Type : Article<br />
Keywords : alpha mangostin, complementary DNA, cytokine, deoxyglucose, gamma interferon inducible protein 10, gamma<br />
mangostin, immunoglobulin enhancer binding protein, insulin, interleukin 6, lipopolysaccharide, mitogen activated protein<br />
kinase, transcription factor AP 1, tritium, tumor necrosis factor alpha, unclassified drug, xanthone derivative;<br />
adipocyte, antiinflammatory activity, antioxidant activity, article, controlled study, cytokine production, dose<br />
response, fruit, gene expression, human, human cell, inflammation, insulin resistance, macrophage, mangosteen, obesity;<br />
Adipocytes, Cell Line, Culture Media, Conditioned, Female, Garcinia mangostana, Humans, Inflammation, Insulin<br />
Resistance, Lipopolysaccharides, Macrophages, Mitogen-Activated Protein Kinase Kinases, NF-kappa B, Peptides, Xanthones;<br />
Garcinia mangostana<br />
<strong>Abstract</strong> : Obesity-associated inflammation is characterized by recruitment <strong>of</strong> macrophages (MΦ) into white adipose tissue (WAT) and<br />
production <strong>of</strong> inflammatory cytokines, leading to the development <strong>of</strong> insulin resistance. The xanthones, α- and γ-mangostin<br />
(MG), are major bioactive compounds found in mangosteen that are reported to have antiinflammatory and antioxidant<br />
properties. Thus, we examined the efficacy <strong>of</strong> MG to prevent lipopolysaccharide (LPS)-mediated inflammation in human MΦ<br />
(differentiated U937 cells) and cross-talk with primary cultures <strong>of</strong> newly differentiated human adipocytes. We found that α-<br />
and γ-MG attenuated LPS-induced expression <strong>of</strong> inflammatory genes, including tumor necrosis factor-α, interleukin-6, and<br />
interferon γ-inducible protein-10 in a dose-dependent manner in MΦ. We also found that α- and γ-MG attenuated LPSactivated<br />
mitogen-activated protein kinases (MAPK) and activator protein (AP)-1, but only γ-MG reduced nuclear factor-κB<br />
(NF-κB). In addition, α- and γ-MG attenuated LPS suppression <strong>of</strong> PPARγ gene expression in a dose-dependent manner.<br />
Notably, the ability <strong>of</strong> MΦ-conditioned media to cause inflammation and insulin resistance in primary cultures <strong>of</strong> human<br />
adipocytes was attenuated by pretreating MΦ with γ-MG. Taken together, these data demonstrate that MG attenuates LPSmediated<br />
inflammation in MF and insulin resistance in adipocytes, possibly by preventing the activation <strong>of</strong> MAPK, NF-κB, and<br />
AP-1, which are central to inflammatory cytokine production in WAT. © <strong>2010</strong> American Society for Nutrition.<br />
No.139<br />
Author (s) : Bunchuailua, W., Zuckerman, I.H., Kulsomboon, V., Suwankesawong, W., Singhasivanon, P., Kaewkungwal, J.<br />
Title : Detection <strong>of</strong> adverse drug reaction signals in the Thai FDA Database: Comparison between reporting odds ratio and Bayesian<br />
confidence propagation neural network methods<br />
Source : Drug Information Journal, Volume 44, Issue 4, July <strong>2010</strong>, Pages 393-403<br />
Document Type : Article<br />
Keywords : Adverse drug reaction; BCPNN; ROR; Signal detection; Spontaneous reporting<br />
<strong>Abstract</strong> : The study aimed to compare performance between the reporting odds ratio (ROR) and the Bayesian confidence propagation<br />
neural network (BCPNN) methods in identifying serious adverse drug reactions (ADRs) using the Thai FDA spontaneous<br />
database. The two methods were retrospectively applied to identify new, serious ADRs reported with antiretroviral therapy<br />
(ART) drugs using the data set between 1990 and 2006. We plotted the ROR and the information component against time to<br />
compare the differential timing <strong>of</strong> signal detection and the pattern <strong>of</strong> signaling over time between these methods. The ROR<br />
and the BCPNN methods identified the associations between ART drugs and serious ADRs at the same time. Both methods<br />
were similar in detecting the first signal <strong>of</strong> a potential ADR. However, the pattern <strong>of</strong> signaling seems relatively different with<br />
each method. Additional analyses <strong>of</strong> different drugs, ADRs, and databases will contribute to increase understanding <strong>of</strong><br />
methods for post-marketing surveillance using spontaneous reporting system. Copyright © <strong>2010</strong> Drug Information Association,<br />
Inc. All rights reserved.<br />
No.140<br />
Author (s) : Bunchuailua, W.; Zuckerman, I; Kulsomboon, V; Suwankesawong, W.; Singhasivanon, P.; Kaewkungwal, J.<br />
53
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : A COMPARISON OF SIGNAL DETECTION PERFORMANCE BETWEEN REPORTING ODDS RATIO AND BAYESIAN CONFIDENCE<br />
PROPAGATION NEURAL NETWORK METHODS ON ADVERSE DRUG REACTION SPONTANEOUS REPORTING DATABASE OF THE THAI<br />
FDA<br />
Source : VALUE IN HEALTH, Volume: 13, Issue: 7, Pages: A508-A508, Published: NOV <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.141<br />
Author (s) : Bunsiripaiboon, P., Sornmayura, P., Wilasrusmee, C., Lertsithichai, P.<br />
Title : The prognostic significance <strong>of</strong> microvessel density in intrahepatic cholangiocarcinoma<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 1, January <strong>2010</strong>, Pages 66-72<br />
Document Type : Article<br />
Keywords : IHCC; Intrahepatic cholangiocarcinoma; MVD; Survival; Tumor angiogenesis; Tumor microvessel density; Tumor<br />
neovascularization<br />
<strong>Abstract</strong> : Objective: Intrahepatic cholangiocarcinoma (IHCC) is the second most common primary cancer <strong>of</strong> the liver. Tumor<br />
angiogenesis seem to play an important role in tumor growth and prognosis <strong>of</strong> cancer patients. The purpose <strong>of</strong> the present<br />
study was to determine the prognostic value <strong>of</strong> tumor microvessel density (MVD) in patients with IHCC. Material and Method:<br />
Clinicopathological prognostic factors, recurrence rate, and survival in 22 patients with IHCC who underwent liver resection for<br />
IHCC were reviewed. Tumor MVD was estimated using immunohistochemical methods. Overall probabilities <strong>of</strong> recurrence and<br />
survival were estimated using Kaplan-Meier methods. Prognostic significance <strong>of</strong> MVD and other factors was tested using Cox<br />
proportional hazards regression. Results: There was no significant association between any clinicopathologic factors (age, sex,<br />
tumor markers, and pathologic factors including MVD) and time-to-tumor recurrence. The only prognostic factor associated<br />
with survival was tumor stage. MVD was neither a significant survival predictor nor a predictor <strong>of</strong> tumor recurrence.<br />
Conclusion: The only factor associated with poor prognosis in patients with IHCC in the present study was higher tumor stage.<br />
MVD was not a significant prognostic factor in patients with IHCC.<br />
No.142<br />
Author (s) : Buntilov, V.<br />
Title : A wavelet-based quality measure for evaluating the degradation <strong>of</strong> pan-sharpened images due to local contrast inversion<br />
Source : Proceedings <strong>of</strong> SPIE - The <strong>International</strong> Society for Optical Engineering, Volume 7529, <strong>2010</strong>, Article number 75290P<br />
Document Type : Conference Paper<br />
Keywords : Image fusion; Image quality evaluation; Pan-sharpening; Wavelet transform<br />
<strong>Abstract</strong> : Pan-sharpened images can effectively be used in various remote sensing applications. During recent years a vast number <strong>of</strong><br />
pan-sharpening algorithms has been proposed. Thus, the evaluation <strong>of</strong> their performance became a vital issue. The quality<br />
assessment <strong>of</strong> pan-sharpened images is complicated by the absence <strong>of</strong> reference data, the ideal image what the<br />
multispectral scanner would observe if it had as high spatial resolution as the panchromatic instrument. This paper presents a<br />
novel method to evaluate the degree <strong>of</strong> local quality degradation in pan-sharpened images, which is the result <strong>of</strong> contrast<br />
inversion <strong>of</strong> the fusing bands. The proposed method does not require a reference image. Firstly, the algorithm identifies the<br />
areas in which the contrast inversion may be confidently detected. Then, based on the found spatial consistency violations,<br />
the quantitative degradation index is calculated for the fused product. The proposed approach was validated with the use <strong>of</strong><br />
very high resolution optical imagery. The experiments have shown that the proposed measure objectively reflects local<br />
quality deterioration <strong>of</strong> pan-sharpened images. <strong>2010</strong> SPIE-IS&T.<br />
No.143<br />
Author (s) : Bunwong, K.<br />
Title : A new approach to pair approximation method for spatial model in ecology<br />
Source : WSEAS Transactions on Mathematics, Volume 9, Issue 10, October <strong>2010</strong>, Pages 768-777<br />
Document Type : Article<br />
Keywords : Ecology; Epidemiology; Lattice model; Multinomial distribution; Pair approximation; Poisson distribution; Spatial<br />
interaction<br />
54
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : A mathematical model using either mean-field approximation or pair approximation has both advantage and limitation. Meanfield<br />
approximation is suitable for describing behaviors <strong>of</strong> a large system; however, it neglects the scale <strong>of</strong> individual<br />
interaction. On the other hand, pair approximation is suitable for studying local interaction especially singles and pairs;<br />
however, the mathematical formulas are still limited. Thus, choosing the right model for the right purpose is recommended.<br />
The objective <strong>of</strong> this paper is to develop the techniques <strong>of</strong> pair approximation by using probability averaged value based on<br />
probability distribution; for example, multinomial distribution and Poisson distribution. These extended formulas are suitable<br />
for a model composed <strong>of</strong> individuals whose event rates are additionally affected by their nearby neighbor. Finally, we apply<br />
these tools to a simple SIS epidemic model.<br />
No.144<br />
Author (s) : Bunwong, K.<br />
Title : Pair approximations for ecological models with additional neighborhood effects<br />
Source : <strong>International</strong> Conference on Applied Mathematics, Simulation, Modelling – Proceedings, <strong>2010</strong>, Pages 165-169<br />
Document Type : Conference Paper<br />
Keywords : Pair approximation; Probability distribution; SIS epidemic model; Spatial model<br />
<strong>Abstract</strong> : The spatial interaction plays an important role in many ecological situations. A lattice model is a popular tool for creating<br />
such a grid structure <strong>of</strong> discrete individuals together with their possible connection. The procedure, however, is very<br />
computation time consuming depending on the model complexity. Therefore, master equations, correlation equations, and<br />
pair approximations are proposed as analytical methods. In this paper, a new approach to pair approximation method is<br />
introduced. The main assumption beyond this approach is that the space average and the probabilistic average are identical<br />
when the total population size is large enough. This technique is suitable for a model composed <strong>of</strong> individuals whose event<br />
rates such as birth rate, death rate, transmission rate, and recovery rate are additionally affected by their nearby neighbor.<br />
We, finally, apply this technique to a simple SIS epidemic model.<br />
No.145<br />
Author (s) : Bunyaratavej, S.<br />
Title : Potassium hydroxide examination in superficial fungal infection <strong>of</strong> the skin: The experience-based procedure<br />
Source : JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY, Volume: 62, Issue: 3, Supplement: S, Pages: AB85-AB85, Published:<br />
MAR <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.146<br />
Author (s) : Buranaamnuay, K., Wongtawan, T., Masuwatana, S., Tummaruk, P., Techakumphu, M.<br />
Title : Intra-uterine and deep intra-uterine insemination using cryopreserved boar semen in spontaneously-ovulating sows<br />
Source : Thai Journal <strong>of</strong> Veterinary Medicine, Volume 40, Issue 2, <strong>2010</strong>, Pages 215-219<br />
Document Type : Article<br />
Keywords : Artificial insemination; Frozen-thawed spermatozoa; Ovulation; Sow<br />
<strong>Abstract</strong> : The present study was performed to investigate the in vivo fertility <strong>of</strong> frozen-thawed (FT) boar semen after intra-uterine (IUI)<br />
and deep intra-uterine insemination (DIUI) in spontaneously-ovulating sows. A total <strong>of</strong> 48 weaned sows were included. The<br />
sows were divided into three groups, i.e. natural mating (NM) (n=30), IUI (n=9) and DIUI (n=9). In the IUI and DIUI groups, the<br />
sows were inseminated twice, at 24 and 36 h after the detection <strong>of</strong> oestrous by IUI with 2 × 109 spermatozoa/dose or DIUI<br />
with 1 × 109 spermatozoa/dose. Transrectal ultrasonography was used to determine the time <strong>of</strong> ovulation after insemination.<br />
The results revealed that the conception as determined by a 24-day non-return rate <strong>of</strong> the sows was 96.6%, 88.8% and<br />
66.6% (p=0.03) and the farrowing rate (FR) was 96.6%, 66.6% and 66.6% (p=0.01) in NM, IUI and DIUI groups, respectively. The<br />
numbers <strong>of</strong> total piglets born per litter were 9.4±2.8, 11.3±2.9 and 7.6±3.1 piglets in the NM, IUI and DIUI groups, respectively<br />
(p=0.10). These data indicate that the spontaneously-ovulating weaned sows inseminated with either IUI or DIUI using a<br />
relatively low numbers <strong>of</strong> FT spermatozoa resulted in a lower FR compared to NM. The total number <strong>of</strong> piglets born per litter<br />
after IUI was higher than DIUI.<br />
55
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.147<br />
Author (s) : Buranajitpirom, D., Asuvapongpatana, S., Weerachatyanukul, W., Wongprasert, K., Namwong, W., Poltana, P.,<br />
Withyachumnarnkul, B.<br />
Title : Adaptation <strong>of</strong> the black tiger shrimp, Penaeus monodon, to different salinities through an excretory function <strong>of</strong> the antennal<br />
gland<br />
Source : Cell and Tissue Research, Volume 340, Issue 3, June <strong>2010</strong>, Pages 481-489<br />
Document Type : Article<br />
Keywords : Adaptation; Antennal gland; ATPase; Black tiger shrimp, Penaeus monodon (Crustacea); Salinity<br />
<strong>Abstract</strong> : Black tiger shrimps (Penaeus monodon) are able to survive and can be reared under various salinities, possibly by the cellular<br />
adaptation <strong>of</strong> their excretory system, particularly the antennal gland, which is known to regulate body fluid in crustaceans.<br />
We have investigated the morphological and biochemical alterations <strong>of</strong> the antennal glands in shrimp reared in 7, 15, or 30<br />
ppt seawater. Drastic changes occur in animals reared under 7 ppt conditions. Ultrastructural studies <strong>of</strong> the antennal gland in<br />
shrimps reared in 7 ppt seawater have revealed that podocytic cells in the coelomosacs ramify with more cytoplasmic<br />
processes forming the filtration slits, and that the tubular labyrinth cells possess more mitochondria in their basal striation<br />
and a wider tubular lumen than those found in the other groups. Many apical cytoplasmic blebs from labyrinth cells have<br />
also been seen in the lumen <strong>of</strong> the labyrinths under 7 ppt conditions, a feature that is not as prominent under the other<br />
conditions. The expression and activity <strong>of</strong> the Na+/K +-ATPase in the antennal gland are also correlated with the surrounding<br />
environment: the lower the salinity, the higher the expression and activity <strong>of</strong> the enzyme. Immunohistochemistry results have<br />
demonstrated the highest staining intensity in the labyrinth cells <strong>of</strong> shrimps reared under 7 ppt conditions. Our findings thus<br />
suggest that one <strong>of</strong> the adaptation mechanisms <strong>of</strong> this shrimp to the surrounding salinity is the regulation <strong>of</strong> Na +/K+-ATPase<br />
expression in the antennal gland, in conjunction with subcellular changes in its excretory cells. © <strong>2010</strong> Springer-Verlag.<br />
No.148<br />
Author (s) : Burana-Osot, J., Pattanapanyasat, K., Soonthornchareonnon, N., Sukapirom, K., Toida, T.<br />
Title : Characterisation and immuno-stimulating activity <strong>of</strong> polysaccharides from Thai medicinal plants<br />
Source : Natural Product Research, Volume 24, Issue 15, 20 September <strong>2010</strong>, Pages 1403-1412<br />
Document Type : Article<br />
Keywords : flow cytometry; immuno-stimulating activity; polysaccharide compositions; Thai medicinal plant<br />
<strong>Abstract</strong> : Water-soluble polysaccharides were isolated from the tubers <strong>of</strong> Butea superba Roxb. and Pueraria candollei Wall. Ex Benth.<br />
var. mirifica (Shaw et Suvat.) C. Niyomdham, the leaves <strong>of</strong> Centella asiatica (L.) Urb, Ocimum basilicum L., Psidium guajava and<br />
Andrographis paniculata (Burn. f.) Nees, the stems <strong>of</strong> Cymbopogon citratus (Stapf ExG), and the fruits <strong>of</strong> Psidium guajava and<br />
Scaphium scaphigerum. The immunological impacts <strong>of</strong> the polysaccharides on T-lymphocyte proliferation in vitro was<br />
investigated by flow cytometric (immun<strong>of</strong>luorescence) analysis using staphylococcal enterotoxin B (SEB) as a positive control.<br />
It was found that the polysaccharides enhanced T-lymphocyte proliferation, ranging from 4.5 to 27.0% at a concentration <strong>of</strong><br />
100 g mL-1, while the activity <strong>of</strong> SEB was 13.3%. The medicinal plants showing the highest immuno-stimulating activity were<br />
the tubers <strong>of</strong> Butea superba Roxb. The water-extracted tubers contained 60.0% (w/w) carbohydrates with 6.6% (w/w) uronic<br />
acid. The major constituent monosaccharides <strong>of</strong> the tubers were 28.2 mol% galactose, 10.5 mol% arabinose and 36.4 mol%<br />
glucose. © <strong>2010</strong> Taylor & Francis.<br />
No.149<br />
Author (s) : Burana-osot, J., Soonthornchareonnon, N., Chaidedgumjorn, A., Hosoyama, S., Toida, T.<br />
Title : Determination <strong>of</strong> galacturonic acid from pomelo pectin in term <strong>of</strong> galactose by HPAEC with fluorescence detection<br />
Source : Carbohydrate Polymers, Volume 81, Issue 2, 11 June <strong>2010</strong>, Pages 461-465<br />
Document Type : Article<br />
Keywords : Galactose; Galacturonic acid; HPAEC-fluorescence detector; Pectin<br />
<strong>Abstract</strong> : Galacturonic acid (GalA) in pomelo pectin was converted to galactose (Gal) by saponification and reduction reaction <strong>of</strong><br />
carboxy group. The characterization <strong>of</strong> the product was investigated by 1H NMR spectroscopy. The reduced pectin was then<br />
hydrolyzed with trifluoroacetic acid (TFA) and analyzed by high-performance anion-exchange chromatography (HPAEC) with<br />
fluorescence detector. The content <strong>of</strong> GalA was calculated from an increased Gal after reaction. The GalA content obtained<br />
from this method was higher than that obtained from HPAEC-pulsed amperometric detection (PAD), which affords to analyze<br />
GalA directly after hydrolysis. The established method was validated and the results showed good linearity, high precision<br />
56
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
and high sensitivity. This method could be further applied for the analysis <strong>of</strong> GalA content in pomelo pectin. © <strong>2010</strong> Elsevier<br />
Ltd. All rights reserved.<br />
No.150<br />
Author (s) : Burana-osot, J., Soonthornchareonnon, N., Hosoyama, S., Linhardt, R.J., Toida, T.<br />
Title : Partial depolymerization <strong>of</strong> pectin by a photochemical reaction<br />
Source : Carbohydrate Research, Volume 345, Issue 9, 16 June <strong>2010</strong>, Pages 1205-1210<br />
Document Type : Article<br />
Keywords : HPAEC-PAD; NMR spectroscopy; Pectin; Photochemical depolymerization<br />
<strong>Abstract</strong> : Complex heterogeneous polysaccharides that comprise pectin were partially depolymerized by a photochemical reaction<br />
using ultraviolet light in the presence <strong>of</strong> titanium dioxide catalyst. In a period <strong>of</strong> 6 h at pH 7, this UV/TiO2 process decreased<br />
the average molecular weight <strong>of</strong> pectin from 400 kDa to 200 kDa. The characterization <strong>of</strong> the partially depolymerized pectin,<br />
which was fractionated by size-exclusion chromatography, was performed by 1H NMR spectroscopy, and the spectra obtained<br />
showed that the resulting oligosaccharides and polysaccharides maintained the intact core structure <strong>of</strong> pectin. The<br />
monosaccharide content and depolymerization pr<strong>of</strong>ile were determined by high-performance anion-exchange<br />
chromatography coupled with pulsed amperometric detection. This controlled photochemical depolymerization technique<br />
might be useful for preparation <strong>of</strong> pectin oligosaccharides as an ingredient in food and pharmaceutical products. © <strong>2010</strong><br />
Elsevier Ltd. All rights reserved.<br />
No.151<br />
Author (s) : Burapapadh, K., Kumpugdee-Vollrath, M., Chantasart, D., Sriamornsak, P.<br />
Title : Fabrication <strong>of</strong> pectin-based nanoemulsions loaded with itraconazole for pharmaceutical application<br />
Source : Carbohydrate Polymers, Volume 82, Issue 2, 5 September <strong>2010</strong>, Pages 384-393<br />
Document Type : Article<br />
Keywords : Itraconazole; Nanoemulsions; Pectin; Simple homogenization<br />
<strong>Abstract</strong> : The aim <strong>of</strong> this study was to prepare nanoemulsions containing itraconazole (ITZ), a poorly water-soluble drug, using pectin<br />
as a polymeric emulsifier. Nanoemulsions were prepared by simple homogenization to avoid high-pressure conditions. The<br />
influences <strong>of</strong> type <strong>of</strong> internal phase, type and concentration <strong>of</strong> pectin on the droplet size, morphology, and zeta potential <strong>of</strong><br />
the pectin-based emulsions were also examined. Nanoemulsions were achieved when chlor<strong>of</strong>orm was used as an internal<br />
phase while using caprylic/capric triglyceride can produce only micron-sized emulsions. Pectin with high degree <strong>of</strong><br />
esterification <strong>of</strong>fered good emulsion properties because <strong>of</strong> its high amount <strong>of</strong> hydrophobic molecules. The droplet size <strong>of</strong><br />
emulsions decreased with the increased pectin concentration. The addition <strong>of</strong> ITZ to the emulsion formulation was essential<br />
to obtain the nano-sized emulsions, resulting from the molecular association between ITZ and pectin. It appears that 3%<br />
(w/w) pectin provided the most stable emulsion with the highest percent creaming. The obtained nanoemulsions may be<br />
subsequently developed as a self-emulsifying drug delivery system. © <strong>2010</strong> Elsevier Ltd. All rights reserved.<br />
No.152<br />
Author (s) : Burgoine, K.L., Bancone, G., Nosten, F.<br />
Title : The reality <strong>of</strong> using primaquine<br />
Source : Malaria Journal, Volume 9, Issue 1, <strong>2010</strong>, Article number 376<br />
Document Type : Article<br />
Keywords : amikacin, ceftazidime, ceftriaxone, chloroquine, cipr<strong>of</strong>loxacin, creatinine, ferrous sulfate, folic acid, gentamicin, meropenem,<br />
piperacillin plus tazobactam, primaquine, urea, antimalarial agent; abdominal pain, Acinetobacter baumannii, adult, anemia,<br />
article, bacterium isolation, blood smear, blood transfusion, breathing rate, case report, clinical examination, cold clammy<br />
skin, confusion, creatinine blood level, fever, fluid resuscitation, follow up, gene mutation, genotype, glucose 6 phosphate<br />
dehydrogenase deficiency, hematocrit, hemizygote, human, hypotension, intravascular hemolysis, leukocytosis, male,<br />
outcome assessment, oxygen saturation, Plasmodium vivax malaria, polymerase chain reaction, restriction fragment length<br />
polymorphism, rigor, tachycardia, tachypnea, urea blood level, urinalysis, vomiting, weakness, drug effect, hemolysis;<br />
Adult, Antimalarials, Chloroquine, Glucosephosphate Dehydrogenase Deficiency, Hemolysis, Humans, Malaria, Vivax, Male,<br />
Primaquine<br />
<strong>Abstract</strong> : Background: Primaquine is currently the only medication used for radical cure <strong>of</strong> Plasmodium vivax infection. Unfortunately,<br />
its use is not without risk. Patients with glucose-6-phosphate dehydrogenase (G6PD) deficiency have an increased<br />
57
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
susceptibility to haemolysis when given primaquine. This potentially fatal clinical syndrome can be avoided if patients are<br />
tested for G6PD deficiency and adequately informed before being treated. Case presentation. A 35-year old male presented<br />
to our clinic on the Thai-Burmese border with a history and clinical examination consistent with intravascular haemolysis. The<br />
patient had been prescribed primaquine and chloroquine four days earlier for a P. vivax infection. The medication instructions<br />
had not been given in a language understood by the patient and he had not been tested for G6PD deficiency. The patient<br />
was not only G6PD deficient but misunderstood the instructions and took all his primaquine tablets together. With<br />
appropriate treatment the patient recovered and was discharged home a week later. Conclusions. Whilst primaquine remains<br />
the drug <strong>of</strong> choice to eradicate hypnozoites and control P. vivax transmission, the risks associated with its use must be<br />
minimized during its deployment. In areas where P. vivax exists, patients should be tested for G6PD deficiency and<br />
adequately informed before administration <strong>of</strong> primaquine. © <strong>2010</strong> Burgoine et al; licensee BioMed Central Ltd.<br />
No.153<br />
Author (s) : Busadee, N., Panijpan, B., Laosinchai, P., Ruenwongsa, P.<br />
Title : Enhancing highschool students' achievement in permutation and combination through nontraditional word problems, sport<br />
problems and probabilistic games<br />
Source : <strong>International</strong> Journal <strong>of</strong> Learning, Volume 17, Issue 7, <strong>2010</strong>, Pages 413-428<br />
Document Type : Article<br />
Keywords : Experimental research; Inquiry; Permutations and combinations; Probabilistic game; Probability; Sport problem; Students<br />
achievement; Word problem<br />
<strong>Abstract</strong> : Probability concepts in combinations and permutations are difficult to grasp, especially when students are taught by formal<br />
mathematical presentation without having gone through sufficient real-life situations. Besides, some <strong>of</strong> the common textbook<br />
problems are not interesting to students, being more <strong>of</strong> math teasers rather than based on real-life situations. In this study<br />
we tested our newly invented real-life probability problems involving permutations and combinations, and simple problems<br />
requiring obtaining the probabilities <strong>of</strong> events by common sense. Personal relevance, sport situations, and games are the<br />
main features <strong>of</strong> these new problems. The participants were four classes <strong>of</strong> highschool students in a rural setting. One class<br />
served as the control, with the traditional problem unit (TU). The other three classes were given different units on non<br />
traditional word problems (NTU), sport problems (SU), and game problems (GU). The results demonstrated that all units<br />
enhanced students' learning. however students' learning by NTU outperformed those in the other classes. GU and SU were<br />
the less successful ones in enhancing students' achievement. The students showed positive attitude toward learning through<br />
authentic problems. Some unexpected results, especially from sport and game problems, are discussed. © Common Ground,<br />
Nutjira Busadee, Bhinyo Panijpan, Parames Laosinchai, Pintip Ruenwongsa.<br />
No.154<br />
Author (s) : Bussaratid, V., Dekumyoy, P., Desakorn, V., Jaroensuk, N., Liebtawee, B., Pakdee, W., Wattanagoon, Y.<br />
Title : Predictive factors for gnathostoma seropositivity in patients visiting the gnathostomiasis clinic at the hospital for tropical<br />
diseases, Thailand during 2000-2005<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 6, November <strong>2010</strong>, Pages 1316-1321<br />
Document Type : Article<br />
Keywords : Gnathostoma; Predictive factor; Seropositivity; Thailand<br />
<strong>Abstract</strong> : This was a retrospective study <strong>of</strong> patients having Gnathostoma antibody testing at the Hospital for Tropical Diseases, Bangkok<br />
during 2000-2005 to investigate predictive factors for Gnathostoma seropositivity in patients attending the Gnathostomiasis<br />
Clinic. Out <strong>of</strong> 849 patients tested, 531 (62.5%) were Gnathostoma seropositive. The median absolute eosinophil counts were<br />
464 (0-16,796) and 326.5 (0-10,971) cells/mm3 in seropositive and seronegative patients, respectively (p500 cells/mm3), and the frequency <strong>of</strong> cutaneous<br />
swellings between seropositive and seronegative patients were all statistically significant. Patients with a history <strong>of</strong> eating raw<br />
meat and a history <strong>of</strong> cutaneous swelling were at 2.1 and 1.8 times more likely to be Gnathostoma seropositive, respectively.<br />
Logistic regression analysis showed eosinophilia was not a predictive factor for Gnathostoma seropositivity.<br />
No.155<br />
Author (s) : Cai, B., Pumijumnong, N., Tan, M., Muangsong, C., Kong, X., Jiang, X., Nan, S.<br />
Title : Effects <strong>of</strong> intraseasonal variation <strong>of</strong> summer monsoon rainfall on stable isotope and growth rate <strong>of</strong> a stalagmite from<br />
northwestern Thailand<br />
58
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Source : Journal <strong>of</strong> Geophysical Research D: Atmospheres, Volume 115, Issue 21, <strong>2010</strong>, Article number D21104<br />
Document Type : Article<br />
Keywords : Annual resolution, Asian monsoon, Climate signals, Decadal variability, High growth rate, High resolution, Instrumental<br />
data, Intraseasonal variations, Monsoon intensity, Monsoon rainfall, Monsoon season, Oxygen isotopic composition, Rate<br />
parameters, Sea surface temperatures, Stable isotopes, Strong correlation, Summer monsoon rainfall, Thailand, Western<br />
Pacific warm pool; Atmospheric temperature, Atmospheric thermodynamics, Caves, Climate change, Growth<br />
rate, Isotopes, Lakes, Oxygen; Rain; atmospheric chemistry, decadal variation, growth rate, isotopic composition, rainfall, sea<br />
surface temperature, seasonal variation, speleothem, stable isotope, stalagmite; Thailand<br />
<strong>Abstract</strong> : An annually laminated stalagmite from Namjang cave (194030N, 981212E), northwestern Thailand, has been analyzed to<br />
investigate the annual resolution climate signal persevered within its oxygen isotopic composition (δ18O) and growth rate<br />
parameters. The cave site is under the influence <strong>of</strong> Asian monsoon, and local rainfall shows noticeable variations through the<br />
monsoon season. Both δ18O and growth rate records, covering the last 105 years, exhibit persistent decadal-scale variability<br />
and can be compared with local instrumental data. Low δ18O values coincide with high growth rates in the specimen and<br />
correspond to higher relative amounts <strong>of</strong> rainfall in later monsoon season (August-October; hereinafter referred to as ASO<br />
rainfall) versus rainfall in early monsoon season (May-July; hereinafter referred to as MJJ rainfall). The strong correlation<br />
between the δ18O value and the 5 year averaged ratio <strong>of</strong> ASO to MJJ rainfall (r = -0.50, p < 0.001) indicates a significant<br />
imprint <strong>of</strong> intraseasonal variation <strong>of</strong> monsoonal rainfall on stalagmite δ 18O. The close resemblance between the<br />
speleothem δ18O record and Western Pacific Warm Pool (WPWP) sea surface temperature (SST) implies that the WPWP may<br />
play an important role on the decadal variability <strong>of</strong> later monsoon rainfall in this region. Unique in its annual chronology,<br />
high-resolution δ18O, and direct comparison with instrumental data, our record shows for the first time that the climate in<br />
northwestern Thailand has undergone decadal-scale variability and speleothem δ18O is a robust proxy for regional monsoon<br />
intensity. Copyright © <strong>2010</strong> by the American Geophysical Union.<br />
No.156<br />
Author (s) : Cairns, J.; Chalkidou, K.; Panpiemras, J.; Suriyawongpaisal, P.; Yothasamut, J.<br />
Title : EVALUATING THE HEALTH TECHNOLOGY ASSESSMENT PROGRAM IN THAILAND<br />
Source : VALUE IN HEALTH, Volume: 13, Issue: 7, Pages: A546-A546, Published: NOV <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.157<br />
Author (s) : Cappellini, M.D., Porter, J., El-Beshlawy, A., Li, C.-K., Seymour, J.F., Elalfy, M., Gattermann, N., Giraudier, S., Lee, J.-W., Chan, L.L.,<br />
Lin, K.-H., Rose, C., Taher, A., Thein, S.L., Viprakasit, V., Habr, D., Domokos, G., Roubert, B., Kattamis, A.<br />
Title : Tailoring iron chelation by iron intake and serum ferritin: The prospective EPIC study <strong>of</strong> deferasirox in 1744 patients with<br />
transfusion-dependent anemias<br />
Source : Haematologica, Volume 95, Issue 4, April <strong>2010</strong>, Pages 557-566<br />
Document Type : Article<br />
Keywords : Iron chelation therapy; Transfusion medicine; Transfusion-dependent anemias<br />
<strong>Abstract</strong> : Background: Following a clinical evaluation <strong>of</strong> deferasirox (Exjade®) it was concluded that, in addition to baseline body iron<br />
burden, ongoing transfusional iron intake should be considered when selecting doses. The 1-year EPIC study, the largest ever<br />
investigation conducted for an iron chelator, is the first to evaluate whether fixed starting doses <strong>of</strong> deferasirox, based on<br />
transfusional iron intake, with dose titration guided by serum ferritin trends and safety markers, provides clinically acceptable<br />
chelation in patients (aged ≥2 years) with transfusional hemosiderosis from various types <strong>of</strong> anemia. Design and Methods: The<br />
recommended initial dose was 20 mg/kg/day for patients receiving 2-4 packed red blood cell units/month and 10 or 30<br />
mg/kg/day was recommended for patients receiving less or more frequent transfusions, respectively. Dose adjustments were<br />
based on 3-month serum ferritin trends and continuous assessment <strong>of</strong> safety markers. The primary efficacy end-point was<br />
change in serum ferritin after 52 weeks compared with baseline. Results: The 1744 patients enrolled had the following<br />
conditions; thalassemia (n=1115), myelodysplastic syndromes (n=341), aplastic anemia (n=116), sickle cell disease (n=80), rare<br />
anemias (n=43) and other transfused anemias (n=49). Overall, there was a significant reduction in serum ferritin from baseline<br />
(-264 ng/mL; P5%) adverse events were<br />
gastrointestinal disturbances (28%) and skin rash (10%). Conclusions: Analysis <strong>of</strong> this large, prospectively collected data set<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
confirms the response to chelation ther- apy across various anemias, supporting initial deferasirox doses based on<br />
transfusional iron intake, with subsequent dose titration guided by trends in serum ferritin and safety markers (clinicaltrials.gov<br />
identifier: NCT00171821). © <strong>2010</strong> Ferrata Storti Foundation.<br />
No.158<br />
Author (s) : Carretero-González, J., Ezquerra, T.A., Amnuaypornsri, S., Toki, S., Verdejo, R., Sanz, A., Sakdapipanich, J., Hsiao, B.S., López-<br />
Manchado, M.A.<br />
Title : Molecular dynamics <strong>of</strong> natural rubber as revealed by dielectric spectroscopy: The role <strong>of</strong> natural cross-linking<br />
Source : S<strong>of</strong>t Matter, Volume 6, Issue 15, 7 August <strong>2010</strong>, Pages 3636-3642<br />
Document Type : Article<br />
Keywords : Chain mobility, Cross-linked natural rubber, Deproteinization, Dielectric relaxation behavior, Low mobility, Modelbased,<br />
Natural Rubber, Naturally occurring, Normal modes, Protein cross-link, Segmental mode, Star polymers;<br />
Centrifugation, Dielectric relaxation, Gels, Molecular dynamics, Phospholipids, Proteins; Rubber<br />
<strong>Abstract</strong> : In order to understand the molecular dynamics <strong>of</strong> natural rubber, the dielectric relaxation behavior <strong>of</strong> its different<br />
components were investigated. These components included: (1) the linear polyisoprene fraction, obtained after<br />
deproteinization and transesterification <strong>of</strong> natural rubber (TE-DPNR), (2) the gel (GEL) fraction, corresponding to pure natural<br />
chain-end cross-linked natural rubber, (3) deproteinized natural rubber (DPNR), in which the protein cross-links at the ω-end<br />
have been removed, and (4) natural rubber (CNR) purified (through centrifugation) but still containing proteins, phospholipids<br />
and the sol phases. The dielectric relaxation behaviour <strong>of</strong> natural rubber revealed a segmental mode (SM) which is not<br />
affected by natural chain-end cross-linking (so-called naturally occurring network) and a normal mode (NM) which depends<br />
on a naturally occurring network. The dynamics <strong>of</strong> the NM, which is associated to chain mobility, seems to be strongly<br />
affected by natural chain-end cross-linking. We propose a model based on a hybrid star polymer in which the low mobility<br />
core (phospholipids) controls the mobility <strong>of</strong> the polyisoprene arms. © <strong>2010</strong> The Royal Society <strong>of</strong> Chemistry.<br />
No.159<br />
Author (s) : Cervenka, T.; Bladwin, K.; de Souza, M. S.; Paris, R.; Koehler, R.; Thongcharoen, P.; Pitisutitthum, P.; Nitayaphan, S.; Suriyanon,<br />
V.; Robb, M.; Michael, N.; Kim, J.; Kijak, G.<br />
Title : Feasibility <strong>of</strong> high-throughput and low-cost high-resolution class I HLA typing <strong>of</strong> HIV vaccine trial cohorts in Southeast Asia<br />
Source : AIDS RESEARCH AND HUMAN RETROVIRUSES, Volume: 26, Issue: 10, Pages: A70-A71, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.160<br />
Author (s) : Chabé, M., Herbreteau, V., Hugot, J.-P., Noemi, B., Deruyter, L., Serge, M., Dei-Cas, E.<br />
Title : Pneumocystis carinii and pneumocystis wakefieldiae in wild rattus norvegicus trapped in Thailand<br />
Source : Journal <strong>of</strong> Eukaryotic Microbiology, Volume 57, Issue 2, March <strong>2010</strong>, Pages 213-217<br />
Document Type : Article<br />
Keywords : PCR; Pneumocystis spp; Rat; Wild fauna<br />
<strong>Abstract</strong> : This work reports for the first time the presence <strong>of</strong> two Pneumocystis species in wild Rattus norvegicus specimens from<br />
Thailand. Pneumocystis DNA was detected in 57.7% (15/26) wild rats without apparent association with typical<br />
pneumocystosis. Pneumocystis carinii was found alone in five rats (19.2%), Pneumocystis wakefieldiae was detected alone in<br />
six rats (23.1%), and two rats were infected by both species (7.7%). In addition, a new P. wakefieldiae variant sequence has<br />
been identified in three wild R. norvegicus specimens caught in the same geographical area. The high frequency <strong>of</strong><br />
Pneumocystis in wild rats documented in this study and the apparent scarcity <strong>of</strong> severe pneumocystosis were consistent with<br />
an efficient circulation <strong>of</strong> rat Pneumocystis species in ecosystems. © <strong>2010</strong> The Author(s).<br />
No.161<br />
Author (s) : Chadchavalpanichaya, N., Intaratep, N.<br />
Title : Exercise behavior and knowledge among the DM type II patients<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 5, May <strong>2010</strong>, Pages 587-593<br />
Document Type : Article<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : DM patients; Exercise behavior; Knowledge <strong>of</strong> physical exercise<br />
<strong>Abstract</strong> : Objective: To study the exercise behavior and knowledge about physical exercise among diabetic patients. The authors<br />
explored the correlation between the exercise behavior and knowledge <strong>of</strong> physical exercise. Material and Method: DM type II<br />
patients aged more than 18 years, who attended the DM clinic, Siriraj Hospital, Bangkok between April and August 2007 were<br />
randomly interviewed by using questionnaires while they were waiting to see their doctors. Results: One hundred and ninety<br />
six patients were interviewed. They were 62 males and 134 females with an average age <strong>of</strong> 60. 5 years. Most <strong>of</strong> them<br />
exercised regularly at least three times a week (65. 8%). Most <strong>of</strong> them exercised by walking (67%). They liked to exercise in<br />
their houses (48. 7%), and in the morning (41. 8%). Health care providers provided knowledge about exercise (58. 2%). The<br />
benefit <strong>of</strong> exercise known the least was that it could increase endorphin release (61. 2%). The aerobic exercise principle<br />
known the least was the proper frequency <strong>of</strong> exercise (50. 5%). In addition, the exercise principle in DM known the least was<br />
that the DM patients should consult their doctors before starting to exercise (49%). Conclusion: Most <strong>of</strong> the DM patients<br />
exercised regularly, but some had insufficient knowledge. The researchers will take the results to provide the adequate<br />
knowledge to the DM patients in the future.<br />
No.162<br />
Author (s) : Chadchavalpanichaya, N., Srisawasdi, G., Suwannakin, A.<br />
Title : The effect <strong>of</strong> calf stretching box on stretching calf muscle compliance: A prospective, randomized single-blinded controlled<br />
trial<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 12, December <strong>2010</strong>, Pages 1470-1479<br />
Document Type : Article<br />
Keywords : Calf muscle; Calf stretching box; Compliance; Gastrosoleus muscle; Stretching<br />
<strong>Abstract</strong> : Primary Objective: To study the effect <strong>of</strong> calf stretching box usage in increasing the compliance <strong>of</strong> performing calf stretching<br />
exercise as compared to the conventional exercise method. Secondary Objective: To study the effect <strong>of</strong> calf stretching box<br />
usage in decreasing the calf muscle tightness and complications as compared to the conventional exercise method. Material<br />
and Method: Eighty patients older than 45 years old with calf muscles tightness were enrolled in a prospective, randomized<br />
single-blinded controlled trial at the out-patient Rehabilitation medicine clinic, Siriraj Hospital, Bangkok, Thailand between<br />
April and August 2009. Patients were randomized into two groups, the study group (stretching by using calf stretching box)<br />
and the control group (stretching by the conventional exercise method). Patients in both groups were asked to hold the<br />
stretch for at least 1 minute and to perform the stretching program at least two times per day, every day for two weeks.<br />
Furthermore, they were asked to record the real frequency and duration <strong>of</strong> their exercise and complications in a logbook<br />
every day. Results: Thirty-eight patients in each group completed the study. The baseline characteristics <strong>of</strong> the patients in<br />
both groups were similar. The study group had higher frequency and longer duration <strong>of</strong> performing calf stretching exercise<br />
than the control group. They also reported more decrease <strong>of</strong> calf muscle tightness with less pain complication (shoulder<br />
pain, knee pain, low back pain, and calf muscle pain) than the control group (p < 0.05). However, there were no significant<br />
differences in the (between before and after) numbers <strong>of</strong> taut bands, trigger points, and tender points <strong>of</strong> calf muscle and<br />
degree <strong>of</strong> ankle range <strong>of</strong> motion between the two groups. Conclusion: Stretching calf muscle with calf stretching box can<br />
increase compliance, decrease calf muscle tightness and decrease complications when compared with the conventional<br />
exercise method.<br />
No.163<br />
Author (s) : Chadsuthi, S., Wong-Ekkabut, J., Triampo, W., Doungchawee, G., Triampo, D.<br />
Title : Comparison <strong>of</strong> the effects <strong>of</strong> UV-A radiation on Leptospira interrogan serovar Bataviae, Canicola and Pomona<br />
Source : African Journal <strong>of</strong> Biotechnology, Volume 9, Issue 21, 24 May <strong>2010</strong>, Pages 3196-3206<br />
Document Type : Article<br />
Keywords : Leptospira interrogans; Leptospirosis; Pathogenic spirochete; Ultraviolet-a<br />
<strong>Abstract</strong> : Motivated by the lack <strong>of</strong> related studies and an insufficient understanding <strong>of</strong> the response <strong>of</strong> pathogenic spirochetes,<br />
including leptospira to ultraviolet-A (UV-A) (or other stresses), we comparatively studied the effects <strong>of</strong> UV-A radiation on the<br />
Leptospira interrogans serovar Bataviae, Canicola and Pomona. The main purpose <strong>of</strong> this work was to investigate the effects<br />
<strong>of</strong> UV-A irradiation-both short term (immediate) and long term (post-irradiation)-on leptospires at different UV-A dosages,<br />
controlled by the duration <strong>of</strong> exposure time. It was observed that survival fractions linearly decrease from 100 to about 70,<br />
60 and 50% for serovar Pomona, Bataviae and Canicola, respectively. This indicates that, for different serovars, UV-A<br />
irradiation has a quantitatively different effects on growth. Short term effects suggest that Pomona may be more resistant to<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
UV-A than the other serovars. Long term effects show that, when compared with the control group, the treated groups <strong>of</strong><br />
bacteria re-grow when the exposure time is equal or lesser than 6 h (~ 2-6), while the groups exposed for 12 h or longer<br />
experienced little change or a slight decrease. This may indicate that UV-A radiation is able to inhibit the growth <strong>of</strong> bacteria,<br />
but does not prevent self-defense from taking place. UV-A radiation's effect on antigenic components was also investigated.<br />
The immunoblotting method was used and the results are supported by scanning electron microscopy (SEM) and<br />
transmission electron microscopy (TEM) results. Possible explanations for these results are discussed. © <strong>2010</strong> Academic<br />
Journals.<br />
No.164<br />
Author (s) : Chaichompoo, P., Bostik, P., Stephenson, S., Udompunturuk, S., Kobkitjaroen, J., Pattanapanyasat, K., Ansari, A.A.<br />
Title : Multiple KIR gene polymorphisms are associated with plasma viral loads in SIV-infected rhesus macaques<br />
Source : Cellular Immunology, Volume 263, Issue 2, <strong>2010</strong>, Pages 176-187<br />
Document Type : Article<br />
Keywords : Genetics; Innate immunity; KIR's; MHC; NK cells; Non-human primates; Polymorphisms; Rhesus macaques; SIV; Viral<br />
loads<br />
<strong>Abstract</strong> : Innate immune mechanisms play a deterministic role in the rate <strong>of</strong> disease progression during acute infection in HIV infected<br />
humans and SIV infection <strong>of</strong> non-human primates. The role NK cells play in mediating such an effect has thus gained<br />
importance. One <strong>of</strong> the major sets <strong>of</strong> molecules that regulate NK cell function are the killer cell immunoglobulin-like<br />
molecules (KIR's). Our laboratory has previously shown an association <strong>of</strong> KIR3DL alleles 13 and 14 with high plasma viral loads<br />
in a cohort <strong>of</strong> SIV-infected rhesus macaques. To gain a more detailed understanding <strong>of</strong> the role <strong>of</strong> KIR polymorphisms, our<br />
laboratory herein conducted studies <strong>of</strong> three additional KIR loci and show that select KIR3DH alleles appear to be more<br />
strongly associated with high plasma viral loads than KIR3DL alleles 13 and 14. In addition, we herein document the existence<br />
<strong>of</strong> additional new alleles for the KIR1D, KIR2DL4, and the KIR3DH loci. © <strong>2010</strong> Elsevier Inc.<br />
No.165<br />
Author (s) : Chaikledkaew, U.; Turongkaravee, S.; Chansirikarnjana, S.; Pongchareonsuk, P.; Krairit, O.<br />
Title : FACTORS ASSOCIATED WITH TOTAL TIME AND COST OF PROVIDING CARE FOR THAI PATIENTS WITH DEMENTIA<br />
Source : VALUE IN HEALTH, Volume: 13, Issue: 7, Pages: A554-A554, Published: NOV <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.166<br />
Author (s) : Chairoungdua, A., Smith, D.L., Pochard, P., Hull, M., Caplan, M.J.<br />
Title : Exosome release <strong>of</strong> β-catenin: A novel mechanism that antagonizes Wnt signaling<br />
Source : Journal <strong>of</strong> Cell Biology, Volume 190, Issue 6, 20 September <strong>2010</strong>, Pages 1079-1091<br />
Document Type : Article<br />
Keywords : beta catenin, CD82 antigen, CD9 antigen, glycogen synthase kinase 3beta, uvomorulin, Wnt protein; animal cell, article, cell<br />
transport, controlled study, dendritic cell, exosome, knockout mouse, mouse, nonhuman, priority journal, protein<br />
degradation, protein secretion, signal transduction, wild type; Animals, Antigens, CD, beta Catenin, Cadherins, Cell<br />
Line, Exosomes, Gene Knockdown Techniques, Genes, Reporter, Glycogen Synthase Kinase 3, Humans, Kangai-1<br />
Protein, Lysosomes, Membrane Glycoproteins, Mice, Models, Biological, Proteasome Endopeptidase Complex, Protein<br />
Processing, Post-Translational, Protein Stability, Protein Transport, Signal Transduction, Transfection, Wnt Proteins; Mus<br />
<strong>Abstract</strong> : CD82 and CD9 are tetraspanin membrane proteins that can function as suppressors <strong>of</strong> tumor metastasis. Expression <strong>of</strong> CD9<br />
and CD82 in transfected cells strongly suppresses β-catenin - mediated Wnt signaling activity and induces a significant<br />
decrease in β-catenin protein levels. Inhibition <strong>of</strong> Wnt/β-catenin signaling is independent <strong>of</strong> glycogen synthase kinase-3β<br />
and <strong>of</strong> the proteasome- and lysosome-mediated protein degradation pathways. CD82 and CD9 expression induces β-catenin<br />
export via exosomes, which is blocked by a sphingomyelinase inhibitor, GW4869. CD82 fails to induce exosome release <strong>of</strong> βcatenin<br />
in cells that express low levels <strong>of</strong> E-cadherin. Exosome release from dendritic cells generated from CD9 knockout<br />
mice is reduced compared with that from wild-type dendritic cells. These results suggest that CD82 and CD9 down-regulate<br />
the Wnt signaling pathway through the exosomal discharge <strong>of</strong> β-catenin. Thus, exosomal packaging and release <strong>of</strong> cytosolic<br />
proteins can modulate the activity <strong>of</strong> cellular signaling pathways. © <strong>2010</strong> Chairoungdua et al.<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.167<br />
Author (s) : Chaisiri, K., Chaeychomsri, W., Siruntawineti, J., Bordes, F., Herbreteau, V., Morand, S.<br />
Title : Human-dominated habitats and helminth parasitism in Southeast Asian murids<br />
Source : Parasitology Research, Volume 107, Issue 4, September <strong>2010</strong>, Pages 931-937<br />
Document Type : Article<br />
Keywords : Acanthocephalus, animal parasitosis, article, cestodiasis, controlled study, forest, gastrointestinal infection, helminthiasis, host<br />
parasite interaction, human, molecular phylogeny, Muridae, nematodiasis, nonhuman, parasite transmission, priority<br />
journal, rat, Rattus argentiventer, Rattus tanezumi, rural area, Southeast Asia, species dominance, species habitat, species<br />
richness, trematodiasis; Animals, Asia, Southeastern, Biodiversity, Ecosystem, Gastrointestinal<br />
Diseases, Helminths, Humans, Muridae, Parasitic Diseases, Animal, Rodent Diseases; Acanthocephala<br />
(worms), Cestoda, Muridae, Nematoda, Rattus argentiventer, Rattus norvegicus, Rattus tanezumi, Rodentia, Trematoda, Vermes<br />
<strong>Abstract</strong> : The effect <strong>of</strong> habitat anthropization is investigated using a comparative analysis based on a literature survey <strong>of</strong> the<br />
gastrointestinal helminths <strong>of</strong> murid rodents described in Southeast Asia (SEA). The literature survey gave 30 references on<br />
helminth diversity concerning 20 murid rodent species. The diversity <strong>of</strong> helminths was high with a total <strong>of</strong> 13 species <strong>of</strong><br />
cestodes, 15 species <strong>of</strong> trematodes, 29 species <strong>of</strong> nematodes and one species <strong>of</strong> acanthocephalans. The highest helminth<br />
species richness was found in Rattus tanezumi, Rattus norvegicus and Rattus argentiventer, all these species were found in<br />
more human-dominated habitats (agricultural areas or human settlements). Helminth species richness was positively linked<br />
across rodent species to the level <strong>of</strong> the anthropization <strong>of</strong> the host environment from forests, agricultural areas to human<br />
settlements. © <strong>2010</strong> Springer-Verlag.<br />
No.168<br />
Author (s) : Chaisiri, K., Bowonwatanuwong, C., Kasettratat, N., Kiertiburanakul, S.<br />
Title : Incidence and Risk Factors for Ten<strong>of</strong>ovir-Associated Renal Function Decline Among Thai HIV-Infected Patients with Low-Body<br />
Weight<br />
Source : CURRENT HIV RESEARCH, Volume: 8, Issue: 7, Pages: 504-509, DOI: 10.2174/157016210793499259, Published: OCT <strong>2010</strong><br />
Document Type : Article<br />
Keywords : Adverse effect; glomerular filtration rate; HIV; risk factor; renal function; ten<strong>of</strong>ovir<br />
<strong>Abstract</strong> : Objectives: We aimed at determining the incidence and factors for ten<strong>of</strong>ovir disoproxil fumarate (TDF)-associated renal<br />
function decline among Thai HIV-infected patients. Methods: Retrospective and prospective cohort studies were conducted.<br />
We enrolled HIV-infected adults who had initiated TDF. Renal function decline was defined by a decrease <strong>of</strong> 25% in<br />
glomerular filtration rate (GFR) from the baseline. Factors associated with the renal function decline were determined.<br />
Results: A total <strong>of</strong> 405 patients with a median (interquartile range, IQR) body weight <strong>of</strong> 56.5 (50.5-65.0) kg were enrolled. All<br />
but four (99%) were antiretroviral treatment-experience patients. A median (IQR) duration <strong>of</strong> receiving TDF was 16 (8-21)<br />
months. Of these, 78 (19.3%) patients had a 25% decrease in GFR with the incidence rate <strong>of</strong> 16.2 per 100 person-years. By<br />
Kaplan-Meier survival analysis, median time to a 25% decrease in GFR was 28 [95% confidence interval (CI) 25.2-30.8] months.<br />
By multiple logistic regression, lower body weight [odds ratio (OR) 1.15 per 5 kg, 95% CI 1.00-1.33], lower body mass index<br />
(BMI) (OR 2.26 per 1 kg/m(2), 95% CI 1.74-2.94), baseline GFR (OR 1.62 per 10 ml/min/1.73m(2), 95% CI 1.39-1.88), protease<br />
inhibitor (OR 2.12, 95% CI 1.15-3.92), and nephrotoxic drug (OR 3.16, 95% CI 1.44-6.98) were statistically significant factors<br />
associated with a 25% decrease in GFR. Conclusions: The study revealed high incidence <strong>of</strong> TDF-associated renal function<br />
decline among patients with low-body weight and BMI. Additional risk factors were baseline GFR, receiving protease inhibitor,<br />
and nephrotoxic drugs. Close monitoring <strong>of</strong> renal function is warranted among patients with these risk factors.<br />
No.169<br />
Author (s) : Chaithirayanon, K., Sobhon, P.<br />
Title : Molecular cloning and characterization <strong>of</strong> two genes encoding 2-Cys peroxiredoxins from Fasciola gigantica<br />
Source : Experimental Parasitology, Volume 125, Issue 2, June <strong>2010</strong>, Pages 106-113<br />
Document Type : Article<br />
Keywords : 2-Cys peroxiredoxin; Fasciola gigantica; In situ hybridization<br />
<strong>Abstract</strong> : In Fasciola species, peroxiredoxin (Prx) serves as the major antioxidant enzyme to remove hydrogen peroxide that is<br />
generated from various metabolic reactions, because the parasites lack catalase, and only express glutathione peroxidases at<br />
minimal levels. We have cloned and characterized two genes, FgPrx-1 and FgPrx-2, belonging to the 2-Cys Prx family, by<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
immunoscreening <strong>of</strong> an expressed adult stage Fasciola gigantica cDNA library using a rabbit anti-serum against its tegumental<br />
antigens. Predicted FgPrx-1 and FgPrx-2 consisted <strong>of</strong> 218 amino acids each with predicted molecular weights at 24.63. kDa and<br />
24.57. kDa, respectively. The two predicted F. gigantica Prx proteins exhibited 98% identity to each other, and 52% identity to<br />
Prx from oxen which is the natural host. A phylogenetic analysis revealed that FgPrx-1 and FgPrx-2 appear to be closely<br />
related to those <strong>of</strong> Fasciola hepatica. The nucleotide sequences <strong>of</strong> FgPrx-2 are 654. bp, which is similar to that cloned from<br />
newly excysted juveniles <strong>of</strong> F. hepatica. The FgPrx genes were found to be constitutively expressed in all developmental<br />
stages, and with a similar pattern. In the adult parasite, FgPrx transcripts were located in the gut epithelial cells, tegument<br />
cells, and cells <strong>of</strong> reproductive organs, including prostate gland, vitelline glands, testis and ovary. In 4-week-old juveniles, a<br />
similar distribution pattern was observed. Metacercaria and newly excysted juveniles exhibited strongest signals for mRNA<br />
transcripts in the gut epithelium, and moderately in the tegumental cells. Because <strong>of</strong> their key role in protecting the parasite<br />
and specificities, these proteins may have immunodiagnostic as well as vaccine potentials. © <strong>2010</strong> Elsevier Inc.<br />
No.170<br />
Author (s) : Chaiwat, O., Sarima, N., Niyompanitpattana, K., Komoltri, C., Udomphorn, Y., Kongsayreepong, S.<br />
Title : Protocol-directed vs. physician-directed weaning fromventilator in intra-abdominal surgical patients<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 8, August <strong>2010</strong>, Pages 930-936<br />
Document Type : Article<br />
Keywords : Intensive care unit; Mechanical ventilation; Nursing; Outcomes; Protocol-directed weaning; Weaning<br />
<strong>Abstract</strong> : Background: Previous studies have demonstrated that protocol-directed weaning is better than physician-directed weaning in<br />
terms <strong>of</strong> shorter duration <strong>of</strong> mechanical ventilation in general critically ill patients. In this prospective, randomized controlled<br />
trial, the authors compared duration <strong>of</strong> mechanical ventilation between protocol based nurse-directed weaning and<br />
physician-directed weaning in patients following intra-abdominal surgery. Material and Method: One hundred intra-abdominal<br />
surgical patients requiring mechanical ventilation for more than 24 hours were randomly assigned to receive either protocoldirected<br />
(n = 51) or physician-directed (n = 49) weaning from mechanical ventilation. Patients assigned to the protocoldirected<br />
weaning group underwent daily screening and a spontaneous breathing trial by nursing staff. Outcomes: The primary<br />
outcome was the duration <strong>of</strong> mechanical ventilation. Results: The median duration <strong>of</strong> mechanical ventilation was 40 and 72<br />
hrs in protocol-directed and physician-directed groups, respectively (p < 0.001). Two patients in the protocol-directed group<br />
and three patients in the physician directed group were re-intubated within the first 72 hours after extubation (p = 0.61).<br />
Conclusion: Daily screening <strong>of</strong> respiratory function in intra-abdominal surgical patients followed by trials <strong>of</strong> spontaneous<br />
breathing performed by nurses resulted in a shorter duration <strong>of</strong> mechanical ventilation when compared to traditional<br />
physician-directed weaning.<br />
No.171<br />
Author (s) : Chaiyarit, S., Mungdee, S., Thongboonkerd, V.<br />
Title : Non-radioactive labelling <strong>of</strong> calcium oxalate crystals for investigations <strong>of</strong> crystal-cell interactions and internalization<br />
Source : Analytical Methods, Volume 2, Issue 10, October <strong>2010</strong>, Pages 1536-1541<br />
Document Type : Article<br />
Keywords : Calcium oxalate crystals, Calcium oxalates, Cell interaction, Dihydrates, Fluorescence microscopes, Ionic dyes, Kidney<br />
stone, Laser scanning, Laser scanning confocal microscopy, MDCK cells, Phase-contrast, Radioactive labelling, SIMPLE<br />
method, Tubular cells; Adhesion, Confocal microscopy, Flow cytometry, Fluorescence, Radioactivity, Visualization; Crystals<br />
<strong>Abstract</strong> : The study <strong>of</strong> interactions between renal tubular cells and calcium oxalate (CaOx) crystals and their internalization was limited<br />
in the past due to lack <strong>of</strong> a simple method for visualization <strong>of</strong> CaOx crystals during such processes. We have developed nonradioactive<br />
techniques for efficiently labelling and imaging CaOx crystals in the study <strong>of</strong> crystal-cell interactions and<br />
internalization. A total <strong>of</strong> 12 ionic dyes, as well as AlexaFluor-488, FITC-conjugated IgG and Cy3-conjugated IgG were used to<br />
stain/label CaOx crystals. Thereafter, the crystals were incubated with MDCK cells for 48 h. The crystal images were obtained<br />
using light, phase-contrast, fluorescence, or laser-scanning confocal microscopy. The internalized CaOx crystals were finally<br />
quantified by flow cytometry. From 12 ionic dyes tested, CaOx monohydrate (COM) crystals were stainable only with CBB-<br />
R250 (blue) and Ponceau-S (red), whereas CaOx dihydrate (COD) crystals were stainable only with CBB-R250 (blue) and CBB-<br />
G250 (blue), which did not stain COM crystals but transformed them to COD. Additionally, only COM could be labelled and<br />
imaged with AlexaFluor-488 (green), FITC-conjugated IgG (green) and Cy3-conjugated IgG (red). Crystal-cell interactions<br />
(indicated by interrupted borders <strong>of</strong> crystals) and adhesion were successfully visualized under a light, phase-contrast, or<br />
fluorescence microscope. Moreover, laser-scanning confocal microscopic examination successfully identified internalized<br />
64
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
crystals, which could be quantified by flow cytometry. These non-radioactive techniques are very simple and effective for<br />
labelling and imaging COM and COD crystals for the study <strong>of</strong> crystal-cell interactions, adhesion and internalization, and will be<br />
very useful to investigate mechanisms <strong>of</strong> kidney stone formation. © <strong>2010</strong> The Royal Society <strong>of</strong> Chemistry.<br />
No.172<br />
Author (s) : Chaiyen, P.<br />
Title : Flavoenzymes catalyzing oxidative aromatic ring-cleavage reactions<br />
Source : ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, Volume: 493, Issue: 1, Special Issue: SI, Pages: 62-70, DOI:<br />
10.1016/j.abb.2009.08.021, Published: JAN <strong>2010</strong><br />
Document Type : Review<br />
Keywords : Pyridoxine; Hydroxypyridine; Hydroxynicotinic acid; Ring-opening; Ring-cleavage; Hydroxylase; Monooxygenase; C4ahydroperoxy<br />
flavin; C4a-hydroxy flavin; Hydroxylation<br />
<strong>Abstract</strong> : 2-Methyl-3-hydroxypyridine-5-carboxylic acid (MHPC) oxygenase (MHPCO) and 5-pyridoxic acid oxygenase are flavoenzymes<br />
catalyzing an aromatic hydroxylation and a ring-cleavage reaction. Both enzymes are involved in biodegradation <strong>of</strong> vitamin B6<br />
in bacteria. Oxygen-tracer experiments have shown that the enzymes are monooxygnases since only one atom <strong>of</strong> molecular<br />
oxygen is incorporated into the products. Kinetics <strong>of</strong> MHPCO has shown that the enzyme is similar to single-component<br />
flavoprotein hydroxylases in that the binding <strong>of</strong> MHPC is required prior to the flavin reduction by NADH, and C4a-hydroperoxy-<br />
FAD and C4a-hydroxy-FAD are found as intermediates. Investigation on the protonation status <strong>of</strong> the substrate upon binding<br />
to the enzyme has shown that only the tri-ionic form <strong>of</strong> MHPC is bound at the MHPCO active site. Using a series <strong>of</strong> FAD<br />
analogues with substituents at the 8-position <strong>of</strong> the isoalloxazine ring, the oxygenation <strong>of</strong> MHPC by the C4a-hydroperoxy-FAD<br />
was shown to occur via an electrophilic aromatic substitution mechanism. Recently. the X-ray structures <strong>of</strong> MHPCO and a<br />
complex <strong>of</strong> MHPC-MHPCO at 2.1 angstrom have been reported and show the presence <strong>of</strong> nine water molecules in the<br />
enzyme active site. Based on structural data, a few residues, Tyr82, Tyr223, Arg181, were suggested to be important for<br />
catalysis <strong>of</strong> MHPCO. (C) 2009 Elsevier Inc. All rights reserved.<br />
No.173<br />
Author (s) : Chamnanchanunt, S.; Ouppapong, T.; Charoenpong, W.; Jearraksuwan, S.; Ruangweerayut, R.; Wattanagoon, Y.; Desakorn, V.<br />
Title : ANEMIA IN ADULT PATIENTS WITH SEVERE FALCIPARUM MALARIA, OUTCOMES AND ASSOCIATED FACTORS<br />
Source : HAEMATOLOGICA-THE HEMATOLOGY JOURNAL, Volume: 95, Supplement: 2, Pages: 715-716, Meeting <strong>Abstract</strong>: 1851,<br />
Published: JUN <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.174<br />
Author (s) : Chamniansawat, S., Chongthammakun, S.<br />
Title : Genomic and non-genomic actions <strong>of</strong> estrogen on synaptic plasticity in SH-SY5Y cells<br />
Source : Neuroscience Letters, Volume 470, Issue 1, 5 February <strong>2010</strong>, Pages 49-54<br />
Document Type : Article<br />
Keywords : Arc; Estrogen receptor; Genomic estrogenic action; Non-genomic estrogenic action; Synaptic plasticity<br />
<strong>Abstract</strong> : Estrogen modulates synaptic plasticity, an important mechanism <strong>of</strong> memory storage. Previously, we have reported that<br />
estrogen rapidly increases the expression <strong>of</strong> Arc (activity-regulated cytoskeleton associated protein), a key protein for synaptic<br />
plasticity, via non-genomic phosphoinositide-3 kinase (PI-3K)-, mitogen-activated protein kinase (MAPK)-, and estrogen receptor<br />
(ER)-dependent pathways in SH-SY5Y cells. The present study aimed to investigate the role <strong>of</strong> each ER subtype, α and β, in<br />
synaptic plasticity in SH-SY5Y cells. The specific agonist <strong>of</strong> ERβ (DPN) markedly induced Arc expression that mimics treatment<br />
with estrogen, but not ERα (PTT). Determination <strong>of</strong> subcellular localization <strong>of</strong> ERβ using immunocytochemistry shows that<br />
ERβ was retained in the cytoplasm <strong>of</strong> the untreated cells. In estrogen-treated cells, the membrane and cytosolic ERβ<br />
gradually decreased, while nuclear ERβ progressively increased in time-dependent manner, suggesting estrogen-dependent<br />
nuclear translocation <strong>of</strong> ERβ. Nuclear accumulation <strong>of</strong> ERβ at 6-12 h post-estrogen treatment, leads to increased PSD-95 and<br />
SYP mRNA expression, indicating the classical genomic estrogenic action on synaptic plasticity. However, the block <strong>of</strong> PI-3K<br />
signaling by Wortmannin partially suppressed estrogen (48 h)-induced PSD-95 and SYP expression, suggesting a crosstalk<br />
mechanism between genomic and non-genomic actions <strong>of</strong> estrogen on synaptic plasticity. Therefore, the estrogen-enhanced<br />
65
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
synaptic plasticity is ERβ-dependent and involves the crosstalk mechanism <strong>of</strong> non-genomic and genomic estrogenic actions.<br />
© 2009 Elsevier Ireland Ltd. All rights reserved.<br />
No.175<br />
Author (s) : Chamniansawat, S., Chongthammakun, S.<br />
Title : MODULATION OF MICROGLIA IN RESPONSE TO PROLONGED EXPOSURE TO HIGH GLUCOCORTICOID LEVEL, IMPLICATION FORM<br />
AN IN VITRO MODEL OF CHRONIC DEPRESSION<br />
Source : JOURNAL OF NEUROCHEMISTRY, Volume: 115, Supplement: 1, Pages: 19-19, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.176<br />
Author (s) : Chamratrithirong, A., Miller, B.A., Byrnes, H.F., Rhucharoenpornpanich, O., Cupp, P.K., Rosati, M.J., Fongkaew, W., Atwood, K.A.,<br />
Chookhare, W.<br />
Title : Spirituality within the family and the prevention <strong>of</strong> health risk behavior among adolescents in Bangkok, Thailand<br />
Source : Social Science and Medicine, Volume 71, Issue 10, November <strong>2010</strong>, Pages 1855-1863<br />
Document Type : Article<br />
Keywords : Adolescents; Alcohol; Buddhism; Family; Health behavior; Risk behavior; Sexual behavior; Spirituality; Thailand; Tobacco<br />
<strong>Abstract</strong> : This study investigates the influences <strong>of</strong> a family's spiritual beliefs and practices on substance use and sexual risk behaviors<br />
among young adolescents 13-14 years old in Bangkok, Thailand. Independent predictor variables are the parents' and teens'<br />
spiritual beliefs and practices in Buddhism and parental monitoring behaviors. The study uses data from the 2007 Baseline<br />
Survey <strong>of</strong> the Thai Family Matters Project, which adapted a U.S. based family prevention program for Thai culture. A<br />
representative sample <strong>of</strong> 420 pairs <strong>of</strong> parents and teens from the Bangkok metropolitan area was recruited to participate in<br />
the study. Structural equation models indicate that positive direct and indirect associations <strong>of</strong> the spirituality <strong>of</strong> parents and<br />
teens within a family and the prevention <strong>of</strong> adolescent risk behaviors are significant and consistent. © <strong>2010</strong> Elsevier Ltd.<br />
No.177<br />
Author (s) : Chamroonsawasdi, K., Suparp, J., Kittipichai, W., Khajornchaikul, P.<br />
Title : Gender roles, physical and sexual violence prevention in primary extend to secondary school in Samutsakorn province,<br />
Thailand<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 3, March <strong>2010</strong>, Pages 358-365<br />
Document Type : Article<br />
Keywords : Gender roles; Violence prevention<br />
<strong>Abstract</strong> : Objective: To enhance positive attitude and life skills on gender roles to prevent physical and sexual violence. Material and<br />
Method: A whole school-based participatory learning program using a quasi-experimental study with pre and post test design<br />
was conducted among 2 schools during June-September, 2005. The experimental group, were 134 students in a primary<br />
school and 179 students in a secondary school. While the control group, were 122 students in a primary school and 95<br />
students in sa econdary school. Results: Means score <strong>of</strong> attitude toward gender roles before implementation in the<br />
experimental group was significantly lower than the control group (p < 0.05). After implementation, the means score in the<br />
experimental group was not significantly different from the control group (p > 0.05). Means paired different score (afterbefore)<br />
between the two groups was significantly different (p = 0.002). Conclusion: A whole school-based program on gender roles<br />
and violence prevention is suitable for youths and should be merged as school curricula and expanded as a nationwide<br />
program at all level <strong>of</strong> education. Gender equity should be taught at an early childhood. Parental involvement in schoolbased<br />
activities should be negotiated.<br />
No.178<br />
Author (s) : Chan, M.A., Gigliotti, N.M., Matangkasombut, P., Gauld, S.B., Cambier, J.C., Rosenwasser, L.J.<br />
Title : CD23-mediated cell signaling in human B cells differs from signaling in cells <strong>of</strong> the monocytic lineage<br />
Source : Clinical Immunology, Volume 137, Issue 3, December <strong>2010</strong>, Pages 330-336<br />
Document Type : Article<br />
Keywords : B cells; Fc receptors; Human; Signal transduction<br />
66
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : CD23 is the low affinity receptor for IgE and in B cells CD23 has been proposed to play a role in the regulation <strong>of</strong> IgE<br />
synthesis. CD23 is expressed also on other cell types including monocytes/macrophages, eosinophils, follicular dendritic cells<br />
and intestinal epithelial cells none <strong>of</strong> which is capable <strong>of</strong> expressing IgE. The diverse nature <strong>of</strong> the expressing cells suggests<br />
that either the CD23-mediated signal transduction pathway may be different among the cell types or biological outcomes<br />
differ in different cells in response to the same signaling pathway. To address this issue, the CD23 signaling pathway was<br />
analyzed and compared in primary tonsillar B cells and in the monocytic cell lines U937 and THP-1. Activation <strong>of</strong> the tyrosine<br />
kinase Fyn and the serine/threonine kinase Akt were only observed in B cells. These results suggest that the CD23-mediated<br />
signal transduction pathways in human B cells and human monocytes are different. © <strong>2010</strong> Elsevier Inc.<br />
No.179<br />
Author (s) : Chanapiwat, P., Kaeoket, K., Tummaruk, P.<br />
Title : The sperm DNA damage after cryopreservation <strong>of</strong> boar semen in relation to post-thawed semen qualities, antioxidant<br />
supplementation and boars effects<br />
Source : Thai Journal <strong>of</strong> Veterinary Medicine, Volume 40, Issue 2, <strong>2010</strong>, Pages 187-193<br />
Document Type : Article<br />
Keywords : Antioxidant; DNA damage; Pig; Sperm<br />
<strong>Abstract</strong> : The objectives <strong>of</strong> the present study were to evaluate the damage <strong>of</strong> DNA <strong>of</strong> the frozen-thawed (FT) boar spermatozoa and to<br />
investigate the effect <strong>of</strong> various concentrations <strong>of</strong> L-cysteine supplementation on the sperm DNA damage. A total <strong>of</strong> 104<br />
cryopreserved semen samples from twenty-six ejaculates <strong>of</strong> 16 proven boars were analyzed. Of these samples, each semen<br />
sample contained a different concentration <strong>of</strong> L-cysteine i.e., 0 (n=41), 5 (n=41), 10 (n=11) and 15 (n=11) mM. All <strong>of</strong> the<br />
semen samples were cryopreserved by controlled-rate freezer. The semen was thawed at 50°C for 12 sec and the damage to<br />
the sperm DNA was determined using acridine orange (AO) staining. The results revealed that, on average, the DNA damage<br />
was observed in 0.5% <strong>of</strong> the FT boar spermatozoa. DNA damage varied among the boars from 0.0% to 4.0%. The levels <strong>of</strong><br />
DNA damage were 0.6%, 0.4%, 0.5% and 0.9% in the extenders supplemented with 0, 5, 10 and 15 mM <strong>of</strong> L-cysteine,<br />
respectively (p>0.05). In conclusion, the DNA damage <strong>of</strong> the FT boar spermatozoa was relatively low. No adverse effect <strong>of</strong> Lcysteine<br />
supplementation up to 10 mM on the damage <strong>of</strong> the sperm DNA was found. Boar characteristic is the most<br />
important factor affecting the damage <strong>of</strong> the sperm DNA.<br />
No.180<br />
Author (s) : Chandanasotthi, P., Rawiworrakul, T., Auemaneekul, N., Sowattanangoon, N., Boonsatean, W., Sunsern, R., Wichitnak, S.,<br />
Ritthuredee, P.<br />
Title : COMMUNITY-BASED PARTICIPATION RESEARCH ON THAI PEOPLE WITH TYPE 2 DIABETES: PRELIMINARY KEYS TO MOBILIZE AND<br />
CHANGE BEHAVIORS<br />
Source : INTERNATIONAL JOURNAL OF BEHAVIORAL MEDICINE, Volume: 17, Supplement: 1, Pages: 244-245, Published: AUG <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.181<br />
Author (s) : Chandrashekar, C.K., Basavalingu, B., Rai, K.M.L., Ananda, S., Tonthai, T., Soga, K., Byrappa, K.<br />
Title : Novel method <strong>of</strong> synthesis <strong>of</strong> R3+:YVO4 (where R=Nd, Er) crystals<br />
Source : Materials Research Innovations, Volume 14, Issue 1, 1 February <strong>2010</strong>, Pages 38-44<br />
Document Type : Conference Paper<br />
Keywords : Ftir; Hydrothemal synthesis; R3+:YVO4 (R=ND, ER); Rare earth vanadates; Sem; X-ray diffraction<br />
<strong>Abstract</strong> : Novel synthesis <strong>of</strong> neodymium and erbium doped yttrium vanadate system under hydrothermal and solvothermal conditions<br />
has been carried out with reference to the starting materials, the solvent media and in turn the resultant products.<br />
Neodymium/erbium doped yttrium orthovanadates <strong>of</strong> nano-to micrometre sized crystals with varying morphologies have<br />
been obtained through hydrothermal and solvothermal reactions. The crystals obtained have been subjected to powder Xray<br />
diffraction, Fourier transform infrared spectroscopy, and morphological and scanning electron microscopic studies. © <strong>2010</strong><br />
W. S. Maney & Son Ltd.<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.182<br />
Author (s) : Changbunjong, T., Jirapattharasate, C., Buddhirongawatr, R., Chewajon, K., Charoenyongyoo, P., Suwanapakdee, S.,<br />
Waengsothorn, S., Triwitayakorn, K., Chaichoun, K., Ratanakorn, P.<br />
Title : Ectoparasitic fauna <strong>of</strong> birds, and volant and non-volant small mammals captured at Srinakarin Dam, Kanchanaburi, Thailand<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 3, May <strong>2010</strong>, Pages 526-535<br />
Document Type : Article<br />
Keywords : Bird; Ectoparasites; Small mammal; Srinakarin dam; Thailand<br />
<strong>Abstract</strong> : The investigation <strong>of</strong> ectoparasitic fauna on birds and volant and nonvolant small mammals at srinakarin dam Kanchanaburi<br />
province Thailand was carried out under a national biodiversity and disease surveillance program for four consecutive<br />
months: January February May and june 2009. a total <strong>of</strong> 122 animals Comprised <strong>of</strong> 15 species <strong>of</strong> birds, 9 species <strong>of</strong> volant<br />
small mammals and 8 species <strong>of</strong> non-volant small mammals were examined for ectoparasite infestation. <strong>of</strong> these animals 1<br />
genus <strong>of</strong> hard ticks (ixodidae), 2 species <strong>of</strong> mesostigmatid mites (laelapidae), 4 genera in three families <strong>of</strong> astigmatid mites<br />
(proctophyllodidae, Pteronyssidae and trouessartiidae),4 species in three families <strong>of</strong> lice (philopteridae Polyplacidae and<br />
trichodectidae) and 2 families <strong>of</strong> batflies (nycteribiidae and streblidae) were collected. This is the first survey conducted to<br />
determine ectoparasites infesting birds and small mammals living in the reserved forest <strong>of</strong> srinakarin dam Thailand. a lower<br />
infestation rate <strong>of</strong> ectoparasites was observed in mammals, Ranging from 3.5% to 10.3% than birds With infestation rates<br />
between 7.3% and 34.2%. no major potential health risks to people who lived in this area were found.<br />
No.183<br />
Author (s) : Changbunjong, T., Weluwanarak, T., Chamsai, T., Sedwisai, P., Ngamloephochit, S., Suwanpakdee, S., Yongyuttawichai, P.,<br />
Wiratsudakul, A., Chaichoun, K., Ratanakorn, P.<br />
Title : Occurrence <strong>of</strong> ectoparasites on rodents in Sukhothai province, Northern Thailand<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 6, November <strong>2010</strong>, Pages 1324-1330<br />
Document Type : Article<br />
Keywords : animal, animal disease, article, disease carrier, ectoparasitosis, flea, mite, parasitology, rodent disease, Thailand, tick;<br />
Animals, Disease Reservoirs, Ectoparasitic Infestations, Mites, Rodent Diseases, Siphonaptera, Thailand, Ticks<br />
<strong>Abstract</strong> : A survey <strong>of</strong> ectoparasites on rodents was carried out bimonthly from April 2008 to March 2009 in 3 districts <strong>of</strong> Sukhothai<br />
Province, northern Thailand. A total <strong>of</strong> 130 rodents comprising 8 species <strong>of</strong> hosts were captured and examined for<br />
ectoparasites. The hosts examined were Bandicota indica, Bandicota savilei, Rattus losea, Rattus rattus, Rattus exulans, Rattus<br />
norvegicus, Menetes berdmorei and Tamiops mcclellandii. Ninety-seven ectoparasites were collected: 1 species <strong>of</strong> tick<br />
(Hemaphysalis bandicota), 2 species <strong>of</strong> mites (Laelaps nuttali and Laelaps echidninus), and 1 species <strong>of</strong> flea (Xenopsylla<br />
cheopis) were identified. The infestation rates by ticks, mites and fleas on the rodents were 0.77, 5.38 and 6.15%,<br />
respectively. Monitoring the rodent population and their ectoparasites is important for future planning <strong>of</strong> prevention and<br />
control <strong>of</strong> zoonotic diseases in the area.<br />
No.184<br />
Author (s) : Changbunjong, T., Wongwit, W., Leemingsawat, S., Tongtokit, Y., Deesin, V.<br />
Title : Effect <strong>of</strong> crude extract <strong>of</strong> Solanum xanthocarpum against snails and mosquito larvae<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 2, March <strong>2010</strong>, Pages 320-325<br />
Document Type : Article<br />
Keywords : Crude extract; Larvicidal activity; Molluscicidal activity; Solanum xanthocarpum<br />
<strong>Abstract</strong> : The ethanolic crude extract from Solanum xanthocarpum was investigated for its molluscicidal activity against Biomphalaria<br />
glabrata, the snail vector <strong>of</strong> Schistosoma mansoni, and Indoplanorbis exustus, the snail vector <strong>of</strong> intestinal echinostomiasis<br />
and Schistosoma spindale, together with the larvicidal activity against the larvae <strong>of</strong> Aedes aegypti, mosquito vector <strong>of</strong> dengue<br />
hemorrhagic fever and Culex quinquefasciatus, the mosquito vector <strong>of</strong> urban bancr<strong>of</strong>tian filariasis. The bioassays were carried<br />
out following the methods recommended by the World Health Organization. For molluscicidal activity, the LC50 against Bi.<br />
glabrata and I. exustus were reported at 163.85 and 198.00 mg/l while the LC90 were 219.33 and 236.80 mg/l, respectively.<br />
Regarding mosquito larvicidal activity, the LC50 against the larvae <strong>of</strong> Ae. aegypti and Cx. quinquefasciatus were 788.10 and<br />
573.20 mg/l, while the LC 90 were 1,288.91 and 1,066.93 mg/l, respectively. These results suggest a preparation <strong>of</strong> ingredients<br />
from this plant may be used as a biological larvicide for these vectors in the field.<br />
68
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.185<br />
Author (s) : Changtam, C., Hongmanee, P., Suksamrarn, A.<br />
Title : Isoxazole analogs <strong>of</strong> curcuminoids with highly potent multidrug-resistant antimycobacterial activity<br />
Source : European Journal <strong>of</strong> Medicinal Chemistry, Volume 45, Issue 10, October <strong>2010</strong>, Pages 4446-4457<br />
Document Type : Article<br />
Keywords : Antimycobacterial activity; Chemical modification; Curcuminoids; Isoxazole analogs<br />
<strong>Abstract</strong> : Curcumin (1), demethoxycurcumin (2) and bisdemethoxycurcumin (3), the curcuminoid constituents <strong>of</strong> the medicinal plant<br />
Curcuma longa L., have been structurally modified to 55 analogs and antimycobacterial activity against Mycobacterium<br />
tuberculosis has been evaluated. Among the highly active curcuminoids, the isoxazole analogs are the most active group,<br />
with mono-O-methylcurcumin isoxazole (53) being the most active compound (MIC 0.09 μg/mL). It was 1131-fold more active<br />
than curcumin (1), the parent compound, and was approximately 18 and 2-fold more active than the standard drugs<br />
kanamycin and isoniazid, respectively. Compound 53 also exhibited high activity against the multidrug-resistant M.<br />
tuberculosis clinical isolates, with the MICs <strong>of</strong> 0.195-3.125 μg/mL. The structural requirements for a curcuminoid analog to<br />
exhibit antimycobacterial activity are the presence <strong>of</strong> an isoxazole ring and two unsaturated bonds on the heptyl chain. The<br />
presence <strong>of</strong> a suitable para-alkoxyl group on the aromatic ring which is attached in close proximity to the nitrogen function <strong>of</strong><br />
the isoxazole ring and a free para-hydroxyl group on another aromatic ring enhances the biological activity. © <strong>2010</strong> Elsevier<br />
Masson SAS. All rights reserved.<br />
No.186<br />
Author (s) : Chanplakorn, N., Chanplakorn, P., Suzuki, T., Ono, K., Chan, M.S.M., Miki, Y., Saji, S., Ueno, T., Toi, M., Sasano, H.<br />
Title : Increased estrogen sulfatase (STS) and 17βhydroxysteroid dehydrogenase type l(17β-HSD1) following neoadjuvant aromatase<br />
inhibitor therapy in breast cancer patients<br />
Source : Breast Cancer Research and Treatment, Volume 120, Issue 3, April <strong>2010</strong>, Pages 639-648<br />
Document Type : Article<br />
Keywords : 17β-hydroxysteroid dehydrogenase; Aromatase inhibitors; Breast cancer; Estrogen sulfatase; Exemestane; Ki67<br />
<strong>Abstract</strong> : Aromatase inhibitors (AIs) are considered the gold standard for endocrine therapy <strong>of</strong> estrogen receptor (ER) positive<br />
postmenopausal breast cancer patients. The therapy may enhance therapeutic response and stabilize disease but resistance<br />
and disease progression inevitably occur in the patients. These are considered at least partly due to an emergence <strong>of</strong><br />
alternative intratumoral estrogen production pathways. Therefore, in this study we evaluated effects <strong>of</strong> exemestane (EXE)<br />
upon the enzymes involved in intratumoral estrogen production including estrogen sulfatase (STS), 17beta;-hydroxysteroid<br />
dehydrogenase type 1 (17β-HSD1), and estrogen sulfotransferase (EST) and correlated the findings with therapeutic responses<br />
including Ki67 labeling index (Ki67). 116 postmenopausal patients with invasive ductal carcinoma, stage II/IIIa, were enrolled in<br />
JFMC34-0601 clinical trials between March, 2006 and January, 2008. EXE <strong>of</strong> 25 mg/day was administered according to the<br />
protocol. Pre- and posttreatment specimens <strong>of</strong> 49 cases were available for this study. Status <strong>of</strong> STS, EST, 17β-HSDl, ER,<br />
progesterone receptor (PgR), human epidermal growth factor receptor type 2 (Her2), and Ki67 in pre- and post-specimens<br />
were evaluated. Specimens examined before the therapy demonstrated following features; ER+ (100%), PgR+ (85.7%), and<br />
Her2+ (77.6%). After treatment, the number <strong>of</strong> Ki67, PgR, and ER positive carcinoma cells demonstrated significant decrement<br />
in clinical response (CIiR) and pathological response (PaR) groups. Significant increment <strong>of</strong> 17β-HSDl and STS immunoreactivity<br />
was detected in all groups examined except for STS in PaR. EST showed significant increment in nonresponsive groups.<br />
Alterations <strong>of</strong> Ki67 <strong>of</strong> carcinoma cells before and after therapy were subclassified into three groups according to its degrees.<br />
Significant alterations <strong>of</strong> intratumoral enzymes, especially 17β-HSD1 and STS, were correlated with Ki67 reduction after<br />
neoadjuvant EXE therapy. This is the first study demonstrating significant increment <strong>of</strong> STS and 17β-HSD1 following AI<br />
neoadjuvant therapy <strong>of</strong> postmenopausal ER positive breast carcinoma patients. This increment may represent the<br />
compensatory response <strong>of</strong> breast carcinoma tissues to estrogen depletion.<br />
No.187<br />
Author (s) : Chanprasertyothin, S., Saetung, S., Rajatanavin, R., Ongphiphadhanakul, B.<br />
Title : Genetic variant in the aquaporin 9 gene is associated with bone mineral density in postmenopausal women<br />
Source : Endocrine, Volume 38, Issue 1, August <strong>2010</strong>, Pages 83-86<br />
Document Type : Article<br />
Keywords : Aquaporin 9 (AQP9); Bone mineral density (BMD); Postmenopausal women<br />
69
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : This study investigated whether single nucleotide polymorphisms (SNP) in the aquaporin 9 (AQP9) gene is associated with<br />
bone mineral density (BMD) in Thai postmenopausal women, after an initial genome-wide screening using high-throughput<br />
SNP genotyping in pooled DNA samples. Subjects consisted <strong>of</strong> 516 postmenopausal women aged 50 or more. Highthroughput<br />
SNP screening was performed by comparing the estimated allele frequency derived from hybridization signal<br />
intensities <strong>of</strong> pooled DNA samples on the Affymetrix 500 K SNP genotyping chip set. The SNP was then genotyped for each<br />
subject individually. Data were expressed as mean ± SEM. Pooled DNA SNP screening revealed the allele frequency <strong>of</strong> an<br />
intronic A/T SNP rs2414539 in the AQP9 gene as being different between subjects with femoral neck BMD in tertiles 1 and 3.<br />
Individual genotyping in all subjects revealed that femoral neck BMD in subjects with TT, TA, and AA genotypes were 0.79 ±<br />
0.06 (n = 3), 0.75 ± 0.01 (n = 98), and 0.71 ± 0.01 g/cm2 (n = 415), respectively. The presence <strong>of</strong> the T allele in rs2414539 was<br />
associated with femoral neck BMD (r = 0.11, P < 0.05) but not with lumbar spine BMD. The relationship was still significant<br />
after controlling for body weight and age (P < 0.05). Genetic variation in the AQP9 gene is associated with femoral neck BMD<br />
in postmenopausal women, and may represent one <strong>of</strong> the susceptibility genes for phenotypes related to bone mass. © <strong>2010</strong><br />
Springer Science+Business Media, LLC.<br />
No.188<br />
Author (s) : Chanrachakul, B., Punyavachira, P., Preechapornprasert, D., Srilar, A., Promsonthi, P.<br />
Title : Randomized Comparison <strong>of</strong> Sublingual and Vaginal Misoprostol for Cervical Ripening at Term<br />
Source : REPRODUCTIVE SCIENCES, Volume: 17, Issue: 3, Supplement: S, Pages: 352A-353A, Meeting <strong>Abstract</strong>: 1001, Published: MAR<br />
<strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.189<br />
Author (s) : Chansamut, N., Buates, S., Udomsangpetch, R., Krasaesub, S., Rachaphaew, N., Takhampunya, R., Sattabongkot, J.<br />
Title : CORRELATION OF AII-TUBULIN AND PFG377 ORTHOLOG GENE EXPRESSIONS IN PLASMODIUM VIVAX GAMETOCYTES AND<br />
MOSQUITO INFECTION<br />
Source : AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, Volume: 83, Issue: 5, Supplement: S, Pages: 119-119, Meeting<br />
<strong>Abstract</strong>: 398, Published: NOV <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.190<br />
Author (s) : Chansela, P., Saitongdee, P., Stewart, P., Soonklang, N., Hanna, P.J., Nuurai, P., Poomtong, T., Sobhon, P.<br />
Title : The tetrapeptide apgw-amide induces somatic growth in haliotis asinina Linnaeus<br />
Source : Journal <strong>of</strong> Shellfish Research, Volume 29, Issue 3, November <strong>2010</strong>, Pages 753-756<br />
Document Type : Conference Paper<br />
Keywords : abalone; APGW-amide; growth; Haliotis asinina; neural ganglia<br />
<strong>Abstract</strong> : APGW-amide is a well-known neurohormone modulator in several molluscs, and is involved in motor activities, feeding, and<br />
sexual behavior. In this report we show that injections <strong>of</strong> APGW-amide into 4-mo-old juvenile Haliotis asinina stimulate growth<br />
<strong>of</strong> body weight and, to a lesser degree, shell length. The injections were given at 0 (control), 20, and 200 ng/g body weight<br />
(BW), at 1-wk intervals for 14 wk. BW and shell length (SL) were measured every week, and growth rates were calculated.<br />
When compared with control animals, there was an approximate 2-fold increase in body growth rates <strong>of</strong> animals given 20<br />
ng/g BW and 200 ng/g BW APGW-amide (P ≤ 0.05), whereas only 20 ng/g BW APGW-amide produced significantly greater SL<br />
than controls (P ≤ 0.05), with an approximate 1.2-fold increase. Using an immunoperoxidase technique, we showed the<br />
presence <strong>of</strong> APGW-amide in neuronal cells <strong>of</strong> the cerebral ganglia and nerve fibers. Overall, these data indicate that APGWamide<br />
is an important neurohormone/neuromodulator in the nervous system <strong>of</strong> H. asinina and plays a role in controlling the<br />
body growth <strong>of</strong> H. asinina. .<br />
No.191<br />
Author (s) : Chansrichavala, P., Chantharaksri, U., Sritara, P., Ngaosuwankul, N., Chaiyaroj, S.C.<br />
70
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Atorvastatin affects TLR4 clustering via lipid raft modulation<br />
Source : <strong>International</strong> Immunopharmacology, Volume 10, Issue 8, August <strong>2010</strong>, Pages 892-899<br />
Document Type : Article<br />
Keywords : Atherosclerosis; Lipid raft; Mevalonate; Statins; Toll-like receptor 4<br />
<strong>Abstract</strong> : Statins, HMG-CoA reductase inhibitors, are used widely in the treatment <strong>of</strong> hypercholesterolemia. Apart from lowering lipid<br />
levels, statins have been shown to have anti-inflammatory effects. Previously we showed that atorvastatin inhibits NF-κB<br />
activation, dose and time dependently, in LPS-TLR4 signaling pathway. In this study, we investigated the anti-inflammatory<br />
mechanism <strong>of</strong> atorvastatin via Toll-like receptor 4 (TLR4) in murine pro-B cell lines transfected with TLR4. Co-treatment <strong>of</strong><br />
LPS-stimulated cells with both atorvastatin and mevalonate rescued NF-κB activation and TLR4 blockade demonstrated that<br />
atorvastatin does not exert its inhibitory effect via TLR4 receptor-ligand binding mechanism. Further investigation into the antiinflammatory<br />
mechanism has shown that atorvastatin causes an impairment <strong>of</strong> TLR4 recruitment into the lipid raft thereby<br />
affecting anti-inflammatory responses. In contrast, mevalonate repaired lipid raft function leading to TLR4 clustering in the<br />
lipid raft. Together, these data suggest that atorvastatin exerts its anti-inflammatory effect via lipid raft modification. This<br />
novel finding <strong>of</strong>fers another insight into the pleiotropic effects <strong>of</strong> atorvastatin and may be applicable to other pattern<br />
recognition receptors that utilize membrane lipid raft as a platform for signal transduction. © <strong>2010</strong> Elsevier B.V.<br />
No.192<br />
Author (s) : Chantasart, D., Li, S.K.<br />
Title : Relationship between the enhancement effects <strong>of</strong> chemical permeation enhancers on the lipoidal transport pathway across<br />
human skin under the symmetric and asymmetric conditions in vitro<br />
Source : Pharmaceutical Research, Volume 27, Issue 9, September <strong>2010</strong>, Pages 1825-1836<br />
Document Type : Article<br />
Keywords : asymmetric; chemical permeation enhancer; human skin; lipoidal pathway; skin transport<br />
experiments; symmetric; transdermal<br />
<strong>Abstract</strong> : Purpose: Previously, the mechanisms <strong>of</strong> action <strong>of</strong> chemical permeation enhancers (CPEs) were studied, and a quantitative<br />
structure-enhancement relationship for the lipoidal transport pathway <strong>of</strong> the stratum corneum was established under<br />
symmetric and equilibrium conditions. The present study examined whether the effects <strong>of</strong> CPEs under the asymmetric<br />
conditions could be predicted by those determined using the symmetric transport experimental approach. Methods: Both<br />
symmetric (same CPE concentration in both donor and receiver chambers) and asymmetric (CPE in the donor chamber only<br />
and phosphate-buffered saline solution in the receiver) transport experiments were carried out in a two-chamber side-by-side<br />
diffusion cell with human epidermal membrane (HEM). Corticosterone was the model permeant to probe the effects <strong>of</strong> CPEs<br />
upon the HEM lipoidal pathway under these conditions. Results: A correlation between the experimental enhancement<br />
factors under the asymmetric conditions (E Asym) and those under the symmetric conditions (E Sym) was observed. The<br />
potencies <strong>of</strong> CPEs based on their donor concentrations are related to their lipophilicities. Conclusions: The results suggest<br />
that the symmetric configuration findings in the previous studies can be used to explain the effects <strong>of</strong> CPEs under the<br />
asymmetric condition likely encountered in practice and to understand drug delivery enhancement in transdermal enhancer<br />
formulation development. © <strong>2010</strong> Springer Science+Business Media, LLC.<br />
No.193<br />
Author (s) : Chanthateyanonth, R., Ruchirawat, S., Srisitthiratkul, C.<br />
Title : Preparation <strong>of</strong> new water-soluble chitosan containing hyperbranched- vinylsulfonic acid sodium salt and their antimicrobial<br />
activities and chelation with metals<br />
Source : Journal <strong>of</strong> Applied Polymer Science, Volume 116, Issue 4, 15 May <strong>2010</strong>, Pages 2074-2082<br />
Document Type : Article<br />
Keywords : Antimicrobial activity; Chitosan; Heavy metals; Hyperbranched polymers; Solubility<br />
<strong>Abstract</strong> : In this work, an efficient and simple method to graft a vinylsulfonic acid sodium salt on a poorly watersoluble chitosan is<br />
described. Commercially available low molecular weight chitosan is converted to water-soluble chitosan containing<br />
hyperbranched-vinylsulfonic acid sodium salt groups. The process comprises the following steps: Michael addition <strong>of</strong> methyl<br />
acrylate, amidation with ethylenediamine, and Michael addition <strong>of</strong> vinylsulfonic acid sodium salt. A variety <strong>of</strong> chitosans<br />
containing vinylsulfonic acid sodium salt, with improved water solubility, is synthesized by repeating these three steps. The<br />
new chitosan derivatives show better antimicrobial activity against Micrococcus luteus ATCC 10240 and Achromobacter<br />
71
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
xylosoxidans ATCC 2706. In addition, they display better chelating behavior with heavy metals, like cadmium(II), copper(II),<br />
and nickel(II), than the starting chitosan. © <strong>2010</strong> Wiley Periodicals, Inc.<br />
No.194<br />
Author (s) : Chantong, B.; Buranasinsup, S.; Toniti, P.; Suttiyotin, P.; Sirimanapong, W.; Nusuetrong, P.<br />
Title : Efficacy <strong>of</strong> extracts <strong>of</strong> Thai medicinal plants as an anesthetics on carp fish (Cyprinus carpio)<br />
Source : PLANTA MEDICA, Volume: 76, Issue: 12, Pages: 1345-1345, Published: AUG <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.195<br />
Author (s) : Chapman, N., Chen, C.-Y., Fujita, T., Hobbs, F.R., Kim, S.-J., Staessen, J.A., Tanomsup, S., Wang, J.-G., Williams, B.<br />
Title : Time to re-appraise the role <strong>of</strong> alpha-1 adrenoceptor antagonists in the management <strong>of</strong> hypertension?<br />
Source : Journal <strong>of</strong> Hypertension, Volume 28, Issue 9, September <strong>2010</strong>, Pages 1796-1803<br />
Document Type : Review<br />
Keywords : alpha-blockers; doxazosin; gastrointestinal therapeutic system; hypertension; metabolic effects<br />
<strong>Abstract</strong> : The role <strong>of</strong> alpha-1 adrenoceptor antagonists (alpha-blockers) in the management <strong>of</strong> hypertension continues to evolve.<br />
Recent data support their use as add-on therapy in uncontrolled hypertension when used in combination with all other<br />
major classes <strong>of</strong> antihypertensive drug and there is increasing evidence suggesting that they have modest but significant<br />
beneficial effects on lipid and glucose metabolism. The availability <strong>of</strong> extended-release formulations has contributed to an<br />
excellent tolerability pr<strong>of</strong>ile. New data from an observational analysis <strong>of</strong> the Anglo-Scandinavian Cardiac Outcomes Trial<br />
(ASCOT) suggest that doxazosin gastrointestinal therapeutic system (GITS) used as a third-line antihypertensive agent lowered<br />
blood pressure and caused modest reductions in plasma lipids. Furthermore, use <strong>of</strong> doxazosin in ASCOT was not associated<br />
with an increased risk <strong>of</strong> heart failure, in contrast to the earlier finding <strong>of</strong> the Antihypertensive and Lipid-Lowering Treatment<br />
to Prevent Heart Attack Trial (ALLHAT). Overall, currently available data support the use <strong>of</strong> alpha-blockers as safe, well<br />
tolerated and effective add-on antihypertensive drugs, which have additional favourable metabolic effects. © <strong>2010</strong> Wolters<br />
Kluwer Health | Lippincott Williams & Wilkins.<br />
No.196<br />
Author (s) : Chareonviriyaphap, T., Grieco, J.P., Suwonkerd, W., Prabaripai, A., Polsomboon, S., Thainchum, K., Sungvornyothin, S., Achee,<br />
N.L.<br />
Title : An improved experimental hut design for the study <strong>of</strong> Aedes aegypti (Diptera: Culicidae) movement patterns in Thailand<br />
Source : Journal <strong>of</strong> Vector Ecology, Volume 35, Issue 2, December <strong>2010</strong>, Pages 428-431<br />
Document Type : Article<br />
Keywords : Aedes, animal, animal behavior, animal housing, article, physiology, Thailand; Aedes, Animals, Behavior, Animal, Housing,<br />
Animal, Thailand; Aedes aegypti, Culicidae, Diptera<br />
<strong>Abstract</strong> : –<br />
No.197<br />
Author (s) : Charoenchai, P., Vajrodaya, S., Somprasong, W., <strong>Mahidol</strong>, C., Ruchirawat, S., Kittakoop, P.<br />
Title : Part 1: Antiplasmodial, cytotoxic, radical scavenging and antioxidant activities <strong>of</strong> thai plants in the family acanthaceae<br />
Source : Planta Medica, Volume 76, Issue 16, <strong>2010</strong>, Pages 1940-1943<br />
Document Type : Article<br />
Keywords : Acanthaceae; antioxidant activity; antiplasmodial activity; cancer chemoprevention; cytotoxic activity; radical scavenging<br />
<strong>Abstract</strong> : Crude extracts (CH2Cl2 and MeOH) <strong>of</strong> 20 plants in the family Acanthaceae were screened for their antiplasmodial, cytotoxic,<br />
antioxidant, and radical scavenging activities. These plants included Asystasia nemorum, Barleria cristata, B. strigosa, Dicliptera<br />
burmanni, Eranthemum tetragonum, Hygrophila ringens, Justicia balansae, J. procumbens, Lepidagathis incurva, Peristrophe<br />
lanceolaria, Phaulopsis dorsiflora, Ruellia kerrii, Strobilanthes auriculata, S. corrugata, S. cusia, S. dimorphotricha, S. karensium,<br />
S. maxwellii, S. pateriformis, and S. brandisii. CH 2Cl2 extracts <strong>of</strong> A. nemorum, S. corrugata, S. cusia, S. maxwellii, S.<br />
pateriformis, and S. brandisii, as well as MeOH extracts <strong>of</strong> J. balansae and J. procumbens, showed antiplasmodial activity with<br />
IC50 values <strong>of</strong> 10100μg/mL. CH2Cl2 extracts <strong>of</strong> nine plants including D. burmanni, H. ringens, J. balansae, J. procumbens, L.<br />
72
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
incurva, P. lanceolaria, P. dorsiflora, S. corrugata, and S. maxwellii showed cytotoxic activity with IC50 values <strong>of</strong> 3.546.0μg/mL.<br />
MeOH extracts (at 100μg/mL) <strong>of</strong> R. kerrii and S. auriculata could effectively scavenge DPPH free radicals (8283% inhibition)<br />
and superoxide anion radicals (79% and 88% inhibition). In the ORAC antioxidant assay, MeOH extracts <strong>of</strong> B. cristata, J.<br />
procumbens, R. kerrii, and S. auriculata exhibited activity with ORAC units <strong>of</strong> 3.13.9. © Georg Thieme Verlag KG Stuttgart · New<br />
York.<br />
No.198<br />
Author (s) : Charoenhirunyingyos, W., Vannasaeng, S.<br />
Title : Pseudohypertriglyceridemia from oral glycerine<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 7, July <strong>2010</strong>, Pages 870-872<br />
Document Type : Article<br />
Keywords : Glaucoma; Glycerine; Pseudohypertriglyceridemia<br />
<strong>Abstract</strong> : A 74 year-old Thai woman was found to have hypertriglyceridemia possibly due to an increased blood glycerol level. Her<br />
previous serum triglyceride (TG) levels were 65 and 99 mg/dl. After 2 months <strong>of</strong> taking glycerine at a dose <strong>of</strong> 50 ml orally<br />
every 8 to 12 hours for treatment <strong>of</strong> glaucoma, her serum TG concentrations increased from 77 to 1,815 and 2,693 mg/dl, but<br />
decreased rapidly to 72 and 59 mg/dl on days 3 and 6 following withdrawal <strong>of</strong> glycerine treatment. There were no other<br />
causes <strong>of</strong> hypertriglyceridemia. High blood glycerol level can interfere with enzymatic methods commonly used in the<br />
measurement <strong>of</strong> TG in most laboratories and result in falsely elevated levels <strong>of</strong> TG.<br />
No.199<br />
Author (s) : Charoenphandhu, N., Wongdee, K., Krishnamra, N.<br />
Title : Is prolactin the cardinal calciotropic maternal hormone?<br />
Soource : Trends in Endocrinology and Metabolism, Volume 21, Issue 7, July <strong>2010</strong>, Pages 395-401<br />
Document Type : Article<br />
Keywords : calcium, prolactin, prolactin receptor; article, bone, bone mass, bone turnover, breast feeding, calcium absorption, calcium<br />
bone level, calcium homeostasis, calcium metabolism, calcium transport, fetus<br />
development, human, hypercalcemia, intestine, kidney, lactation, mammary gland, maternal care, milk<br />
production, osteolysis, osteoporosis, pregnancy, priority journal, prolactin blood level, protein expression; Bone and<br />
Bones, Bone Resorption, Calcium, Calcium, Dietary, Female, Humans, Infant, Newborn, Intestinal Absorption, Intestines, Ion<br />
Transport, Lactation, Pregnancy, Prolactin, Signal Transduction<br />
<strong>Abstract</strong> : To produce <strong>of</strong>fspring, mothers require a large amount <strong>of</strong> calcium for fetal growth and milk production. Increased calcium<br />
demand leads to enhanced intestinal calcium absorption and stockpiling <strong>of</strong> bone calcium in pregnancy prior to<br />
demineralization in lactation. These coordinated events must be carefully organized by calciotropic hormone(s), but the<br />
classical hormones, namely 1,25-dihydroxyvitamin D3, parathyroid hormone and calcitonin, do not appear to be responsible.<br />
Plasma prolactin (PRL) levels are elevated during pregnancy and, in view <strong>of</strong> the presence <strong>of</strong> PRL receptors in gut, bone and<br />
mammary glands, as well as recent evidence <strong>of</strong> the stimulatory effects <strong>of</strong> PRL on intestinal calcium transport, bone<br />
resorption and mammary calcium secretion, we postulate that PRL is the cardinal calciotropic hormone during pregnancy and<br />
lactation. © <strong>2010</strong> Elsevier Ltd.<br />
No.200<br />
Author (s) : Charoensak, A., Pongpornsup, S., Suthikeeree, W.<br />
Title : Wall thickness and outer diameter <strong>of</strong> the normal appendix in adults using 64 slices multidetector CT<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 12, December <strong>2010</strong>, Pages 1437-1442<br />
Document Type : Article<br />
Keywords : Adults; Appendix; Computed tomography; Multidetector<br />
<strong>Abstract</strong> : Objective: Determine wall thickness and outer diameter <strong>of</strong> the normal appendix in adults using 64 slices multidetector CT<br />
Material and Method: A retrospective review <strong>of</strong> 538 patients (190 men, 348 women; mean age, 56.5 years + 15.2 SD) who<br />
were sent for abdomino-pelvic CT without clinical suspicion <strong>of</strong> acute appendicitis was conducted. Two radiologists blinded to<br />
the presented patient's history <strong>of</strong> appendectomy interpreted CT images regarding the visualization <strong>of</strong> the appendix, location,<br />
luminal contents and presence <strong>of</strong> appendicoliths. The maximum outer diameter and two walls thickness <strong>of</strong> the visualized<br />
appendices were measured. Results: The sensitivity, specificity, positive and negative predictive value, and accuracy for<br />
visualization <strong>of</strong> the appendix were 90.8%, 100%, 100%, 76.5% and 92.9% for reader 1 and 97.3%, 97.6%, 99.3%, 91.7% and<br />
73
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
97.4% for reader 2. The mean maximum outer diameter <strong>of</strong> the appendix was 6.6 mm + 1.5 (range, 3.4-14.0 mm). The mean<br />
two walls thickness <strong>of</strong> the appendix was 4.4 mm + 1.0 (range, 1.6-8.0 mm). Conclusion: The normal appendix is frequently<br />
identified at 64 slices MDCT. Contrast to maximum outer diameter, normal two walls thickness <strong>of</strong> the appendix is infrequently<br />
exceeding the threshold <strong>of</strong> 6 mm.<br />
No.201<br />
Author (s) : Charoenteeraboon, J., Ngamkitidechakul, C., Soonthornchareonnon, N., Jaijoy, K., Sireeratawong, S.<br />
Title : Antioxidant activities <strong>of</strong> the standardized water extract from fruit <strong>of</strong> phyllanthus emblica linn<br />
Source : Songklanakarin Journal <strong>of</strong> Science and Technology, Volume 32, Issue 6, <strong>2010</strong>, Pages 599-604<br />
Document Type : Article<br />
Keywords : ABTS•+; Antioxidant; DPPH; Free radical; Phyllanthus emblica linn<br />
<strong>Abstract</strong> : Phyllanthus emblica Linn. is widely used in Thai traditional medicine for treatment <strong>of</strong> various diseases. The fruit <strong>of</strong> P. emblica<br />
is known as a rich source <strong>of</strong> vitamin C, and also contains a mixture <strong>of</strong> phenolic compounds. In this study, the standardized<br />
water extract <strong>of</strong> P. emblica fruit was prepared according to Thai Herbal Pharmacopoeia. Total polyphenol contents <strong>of</strong> the<br />
extract were equivalent to 34.22±1.74 g gallic acid/100g extract. Antioxidant activities <strong>of</strong> the P. emblica extract were<br />
evaluated by several methods, including DPPH and ABTS•+ radical scavenging assays and FRAP assays. The results showed<br />
that the extract has an ability <strong>of</strong> scavenging radicals generated by both DPPH and ABTS•+. Similar to Trolox, the water extract<br />
<strong>of</strong> P. emblica fruit also had a ferric reducing property. Additionally, the extract effectively inhibited H2O2-induced free radical<br />
production in human myeloleukemic U937 cells as measured by 2,7-DCF-DA. The results imply that the fruits <strong>of</strong> P. emblica<br />
are potential sources <strong>of</strong> natural antioxidants, which have free radical scavenging activity and might be useful for hepato-,<br />
cyto-, and radio- protection, as well as reducing oxidative stress in many pathological conditions.<br />
No.202<br />
Author (s) : Charoenying, Y., Kamchaisatian, W., Atamasirikul, K., Direkwattanachai, C., Manuyakorn, W., Benjaponpitak, S.<br />
Title : Cytokine responses during exacerbation compared with stable phase in asthmatic children<br />
Source : Asian Pacific Journal <strong>of</strong> Allergy and Immunology, Volume 28, Issue 1, March <strong>2010</strong>, Pages 35-40<br />
Document Type : Article<br />
Keywords : corticosteroid, gamma interferon, house dust allergen, interleukin 10, interleukin 4, phytohemagglutinin;<br />
adolescent, article, asthma, cell level, cell stimulation, child, clinical article, controlled study, cytokine production, cytokine<br />
response, disease exacerbation, enzyme linked immunosorbent assay, female, human, human<br />
cell, immunoregulation, male, peripheral blood mononuclear cell, school child, supernatant; Adolescent, Antigens,<br />
Dermatophagoides, Asthma, Cells, Cultured, Child, Cytokines, Disease Progression, Female, Humans, Leukocytes,<br />
Mononuclear, Male, Phytohemagglutinins, Severity <strong>of</strong> Illness Index, Skin Tests<br />
<strong>Abstract</strong> : Bronchial asthma is a chronic inflammatory disorder <strong>of</strong> the airways. Balancing in Th1 and Th2 response is a target in the<br />
treatment. Recent studies show that interleukin-10 (IL-10) has an important role in the regulation <strong>of</strong> Th2 and allergic<br />
responses and its amount was found to decrease in asthmatic patients. This study was to focus on cytokine responses,<br />
including interferon-gamma (IFN- γ), IL-4 and IL-10 in asthmatic children during acute exacerbation compared to stable<br />
period. Peripheral blood mononuclear cells (PBMCs) from fourteen asthmatic children during exacerbation and stable phase<br />
were stimulated with phytohemagglutinin (PHA) and mite allergen (Der p) for 72 hours. Levels <strong>of</strong> IFN-γ, IL-4 and IL-10 in cell<br />
culture supernatants were measured using enzyme-linked immunosorbent assay. The median level <strong>of</strong> IL-10 in PBMCs<br />
stimulated with PHA was significantly lower in acute asthma exacerbation compared with stable phase (464 vs. 859.5 pg/ml,<br />
p= 0.03). However, there was no difference in the level <strong>of</strong> IL-10 in PBMCs stimulated with Der p. The level <strong>of</strong> IFN-γ and IL-4<br />
were not different between exacerbation and stable phase both in PHA and Der p-stimulated PBMCs. The decrease <strong>of</strong> IL-10<br />
pro-duction in asthmatic children during acute exacerbation may emphasize the role <strong>of</strong> IL-10 in immune regulation in allergic<br />
disease.<br />
No.203<br />
Author (s) : Charuluxananan, S., Saengchote, W., Klanarong, S., Punjasawadwong, Y., Chau-In, W., Lawthaweesawat, C., Werawatganona, T.<br />
Title : Quality and patient safety in anesthesia service: Thai survey<br />
Source : Asian Biomedicine, Volume 4, Issue 3, June <strong>2010</strong>, Pages 395-401<br />
Document Type : Article<br />
Keywords : Anesthesia; Cardiac arrest; Guidelines; Patient information; Quality; Safety<br />
74
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : Background: The Royal College <strong>of</strong> Anesthesiologists <strong>of</strong> Thailand (RCAT) performed large-scale epidemiologic study <strong>of</strong><br />
anesthesia-related complications and national incidents study in 2004 and 2007, respectively. Objectives: Evaluate the<br />
anesthesia service in Thailand with regard to status <strong>of</strong> quality and patient safety. Material and methods: A pre-planned<br />
structured questionnaire regarding demographic, administrative, preanesthetic, intraoperative postanesthetic variables and<br />
complications were requested to be filled in by nurse anesthetists attending the refresher course lecture <strong>of</strong> RCAT in February<br />
2008. Descriptive statistics was used. Results: Three hundred fifty questionnaires were given and 341 respondents (97%)<br />
returned the questionnaires. Most <strong>of</strong> the respondents (90%) worked in government section. Thirty percent <strong>of</strong> respondents<br />
practiced in hospital without medical doctor anesthesiologist and 58% <strong>of</strong> nurse anesthetists worked in hospitals that have<br />
been accredited. Forty-six percent <strong>of</strong> respondents reported unavailability <strong>of</strong> a 24-hour recovery room. The questionnaires<br />
revealed <strong>of</strong> inadequacy <strong>of</strong> anesthesia personnel (64%), inadequate supervision during emergency condition (53%), inadequacy<br />
<strong>of</strong> patient information regarding anesthesia (57-69%), and low opportunity for patient to choose choice <strong>of</strong> anesthesia (19%).<br />
The commonly used monitoring were pulse oximeter (92% <strong>of</strong> respondents) and electrocardiography (63%). One-third (32%) <strong>of</strong><br />
respondents had to provide <strong>of</strong> anesthesia for patients with insufficient NPO (non per oral) time. Common problems that the<br />
respondents experienced were miscommunication (49%), intraoperative cardiac arrest during the past year (35%), error<br />
related to infusion pump (24%) and medication error (8%). Fifty-five percent <strong>of</strong> respondents had to monitor at least one<br />
patient per month receiving spinal anesthesia. Conclusion: Suggested strategies for quality and patient safety improvement in<br />
anesthesia service are increasing personnel, increasing 24-hour recovery room, improvement <strong>of</strong> supervision, improvement <strong>of</strong><br />
communication, compliance to guidelines and improvement <strong>of</strong> nurse anesthetist's training regarding monitoring patient<br />
receiving spinal anesthesia and cardiopulmonary resuscitation.<br />
No.204<br />
Author (s) : Chatameteekul, W.; Jirapongsananurak, O.; Umrod, P.; Boonchoo, S.; Pacharn, P.; Visitsunthron, N.; Vichyanond, P.;<br />
Luangwedchakarn, V.<br />
Title : The Pneumococcal Antibody Responses in Thai Children with Recurrent/Chronic Sinusitis: a Preliminary Report<br />
Source : JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, Volume: 125, Issue: 2, Supplement: 1, Pages: AB202-AB202, Published:<br />
FEB <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.205<br />
Author (s) : Chatchaisak, D.; Srikiatkhachorn, A.; Govitrapong, P.; Chetsawang, B.<br />
Title : CALCITONIN GENE-RELATED PEPTIDE INDUCES TRANSIENT RECEPTOR POTENTIAL VANILLOID-1 EXPRESSION IN RAT TRIGEMINAL<br />
GANGLION<br />
Source : JOURNAL OF NEUROCHEMISTRY, Volume: 115, Supplement: 1, Pages: 70-70, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.206<br />
Author (s) : Chatdarong, K., Thuwanut, P., Manee-in, S., Lohachit, C., Axnér, E.<br />
Title : Effects <strong>of</strong> thawing temperature and post-thaw dilution on the quality <strong>of</strong> cat spermatozoa<br />
Source : Reproduction in Domestic Animals, Volume 45, Issue 2, April <strong>2010</strong>, Pages 221-227<br />
Document Type : Article<br />
Keywords : animal, animal disease, article, cat, cryopreservation, cytology, freezing, male, physiology, sperm preservation, spermatozoon,<br />
temperature, time; Animals, Cats, Cryopreservation, Freezing, Male, Semen Preservation, Spermatozoa, Temperature, Time<br />
Factors; Arachis hypogaea<br />
<strong>Abstract</strong> : The present study aimed to compare cat sperm quality after thawing using two different temperatures (37 and 70°C) and to<br />
investigate the effects <strong>of</strong> post-thaw dilution on the sperm quality and longevity <strong>of</strong> ejaculated cat spermatozoa. Six ejaculates<br />
<strong>of</strong> each <strong>of</strong> six male cats were collected using an electroejaculator (total 36 ejaculates). The semen was frozen in 0.25-ml<br />
straws using a Tris egg yolk extender containing Equex STM paste. Four straws prepared from each ejaculate were thawed at<br />
four different occasions; (i) at 37°C for 15 s, (ii) at 37°C for 15 s and diluted 1: 2 with Tris buffer (v/v), (iii) at 70°C for 6 s, (iv) at<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
70°C for 6 s and diluted 1: 2 with Tris buffer (v/v). The percentages <strong>of</strong> motile spermatozoa, the scores <strong>of</strong> progressive motility,<br />
the percentages <strong>of</strong> spermatozoa with intact plasma membrane (using SYBR-14/EthD-1 stains) and intact acrosome (using<br />
fluorescein isothiocyanate conjugated peanut agglutinin/propidium iodide stains) were evaluated in fresh semen at 0, 2, 4 and<br />
6 h after thawing. The thawing temperature had no effect on any sperm parameters throughout the incubation period (p ><br />
0.05). The dilution after thawing improved sperm motility, progressive motility and acrosome integrity (p < 0.05). The thawing<br />
<strong>of</strong> cat spermatozoa and subsequently diluting with Tris buffer resulted in an immediate (at 0 h) overall (combined over<br />
temperature) percentage <strong>of</strong> motile sperm <strong>of</strong> 64.8 ± 10.7 (mean ± SD), a score <strong>of</strong> progressive motility <strong>of</strong> 4.0 ± 0.5, a<br />
percentage <strong>of</strong> spermatozoa with intact plasma membrane <strong>of</strong> 64.4 ± 12.1 and intact acrosome <strong>of</strong> 44.8 ± 20.2. In conclusion,<br />
frozen cat semen can be thawed either at 37 or 70°C and post-thaw dilution is recommended to reduce the toxic effect <strong>of</strong><br />
some ingredients in the extender during post-thaw incubation. © 2008 The Authors. Journal compilation © 2008 Blackwell<br />
Verlag.<br />
No.207<br />
Author (s) : Chatthong, B., Onjun, T., Singhsomroje, W.<br />
Title : Model for toroidal velocity in H-mode plasmas in the presence <strong>of</strong> internal transport barriers<br />
Source : Nuclear Fusion, Volume 50, Issue 6, <strong>2010</strong>, Article number 064009<br />
Document Type : Article<br />
Keywords : Density pr<strong>of</strong>ile, Empirical approach, Flow shear, Internal transport barriers, Ion temperature, Model-based, Nclass,<br />
Neoclassical transport, Plasma currents, Predictive modelling, Root mean square errors, Semi-empirical, Statistical<br />
analysis, Time evolutions, Transport models, Velocity model; Electric discharges, Models, Plasmas, Velocity; Computer<br />
simulation<br />
<strong>Abstract</strong> : A model for predicting toroidal velocity in H-mode plasmas in the presence <strong>of</strong> internal transport barriers (ITBs) is developed<br />
using an empirical approach. In this model, it is assumed that the toroidal velocity is directly proportional to the local ion<br />
temperature. This model is implemented in the BALDUR integrated predictive modelling code so that simulations <strong>of</strong> ITB<br />
plasmas can be carried out self-consistently. In these simulations, a combination <strong>of</strong> a semi-empirical mixed Bohm/gyro-Bohm<br />
(mixed B/gB) core transport model that includes ITB effects and NCLASS neoclassical transport is used to compute a core<br />
transport. The boundary is taken to be at the top <strong>of</strong> the pedestal, where the pedestal values are described using a theorybased<br />
pedestal model based on a combination <strong>of</strong> magnetic and flow shear stabilization pedestal width scaling and an<br />
infinite-n ballooning pressure gradient model. The combination <strong>of</strong> the mixed B/gB core transport model with ITB effects,<br />
together with the pedestal and the toroidal velocity models, is used to simulate the time evolution <strong>of</strong> plasma current,<br />
temperature and density pr<strong>of</strong>iles <strong>of</strong> 10 JET optimized shear discharges. It is found that the simulations can reproduce an ITB<br />
formation in these discharges. Statistical analyses including root mean square error (RMSE) and <strong>of</strong>fset are used to quantify the<br />
agreement. It is found that the averaged RMSE and <strong>of</strong>fset among these discharges are about 24.59% and -0.14%, respectively.<br />
© <strong>2010</strong> IAEA, Vienna.<br />
No.208<br />
Author (s) : Chau-In, W., Hintong, T., Rodanant, O., Lekprasert, V., Punjasawadwong, Y., Charuluxananan, S., Tanudsintum, S.<br />
Title : Anesthesia-related complications <strong>of</strong> caesarean delivery in Thailand: 16,697 cases from the Thai anaesthesia incidents study<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, 93 (11), pp. 1274-1283.<br />
Document Type : Article<br />
Keywords : Anesthesia; Caesarean delivery; Cause; Complications; Incidence<br />
<strong>Abstract</strong> : Background: Maternal complications related to anesthesia are low in comparison with the results from obstetric factors in<br />
developing countries. The purposes <strong>of</strong> the present study were to determine the incidence <strong>of</strong> maternal mortality related to<br />
anesthesia, to analyze the causes and to suggest measures to improve anesthetic safety for the parturients. Material and<br />
Method: The present study was part <strong>of</strong> a multi-center study conducted by the Royal College <strong>of</strong> Anesthesiologists <strong>of</strong> Thailand<br />
aimed at surveillance <strong>of</strong> anesthesia-related complications in Thailand. The authors conducted a prospective survey <strong>of</strong><br />
hospital records from all <strong>of</strong> the cases in and outside the operating room receiving general anesthesia in 18 centers between<br />
March 1, 2003 and February 28, 2004. All the forms were checked and verified by three-peer review then included in the<br />
analysis, using descriptive statistics. Results: Sixteen thousand six hundred ninety seven cases were included. The incidence <strong>of</strong><br />
anesthetic complication in parturients was 35.9:10,000 (95% CI 27.4, 46.1). Incidence <strong>of</strong> the four most common anesthetic<br />
related adverse events in caesarean section were desaturation 13.8 (95% CI 8.7, 20.7), cardiac arrest 10.2 (95% CI 5.9, 16.3),<br />
awareness 6.6 (95% CI 3.3, 11.8), and death related anesthesia 4.8 (95% CI 2.17, 9.4). Of these, seven (17.5%) had<br />
76
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
preeclampsia/eclampsia and 46 (76.7%) presented for emergency caesarean delivery. General anesthesia was used in 41<br />
patients (68.4%) and spinal in eighteen (30%). There were eight maternal deaths including five with general anesthesia, giving<br />
a case fatality rate <strong>of</strong> 0.1% <strong>of</strong> general anesthetics or 0.3% <strong>of</strong> caesarean deliveries. Conclusion: The authors found that<br />
inexperience, inadequate knowledge, inadequate care, and patient conditions were the major contributory factors. Most <strong>of</strong><br />
them were preventable and correctable. Additional training and quality assurance can improve and prevent these serious<br />
adverse events.<br />
No.209<br />
Author (s) : Cha-Um, S., Yooyongwech, S., Supaibulwatana, K.<br />
Title : Water deficit stress in the reproductive stage <strong>of</strong> four Indica rice (Oryza sativa L.) genotypes<br />
Source : Pakistan Journal <strong>of</strong> Botany, Volume 42, Issue 5, October <strong>2010</strong>, Pages 3387-3398<br />
Document Type : Article<br />
Keywords : Oryza sativa<br />
<strong>Abstract</strong> : Relative water content (RWC) in the leaf <strong>of</strong> different rice cultivars dropped significantly in relation to reduced soil water<br />
content (SWC), especially in PT1 and IR20. In contrast, the proline content in the leaf-blade and leaf-sheath increased when<br />
plants were subjected to 7% SWC. The RWC was positively related to chlorophyll degradation. Chlorophyll a, chlorophyll b,<br />
total chlorophyll, total carotenoids, maximum quantum yield <strong>of</strong> PSII, stomatal conductance and water use efficiency in rice<br />
grown under water-deficit conditions declined significantly in comparison to the control group, leading to a reduction in netphotosynthetic<br />
rate. In addition, when exposed to water-deficit, panicle length and fertile grains in KDML105 and NSG19 were<br />
stabilized, leading to greater productivity than in PT1 and IR20. These data were utilized as effective criteria for the<br />
classification <strong>of</strong> water-deficit tolerance. From the results, KDML105 and NSG19 were identified as water deficit-tolerant, and<br />
PT1 and IR20 as water deficit-susceptible.<br />
No.210<br />
Author (s) : Chaveepojnkamjorn, W., Pichainarong, N.<br />
Title : Factors associated with alcohol consumption among male high school students in central Thailand<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 3, May <strong>2010</strong>, Pages 735-742<br />
Document Type : Article<br />
Keywords : Alcohol consumption; Associated factors; High school students; Thailand<br />
<strong>Abstract</strong> : A cross-sectional study was conducted to explore factors associated with alcohol consumption among male high school<br />
students in central Thailand. Five thousand one hundred eighty-four male students from central Thailand were classified into<br />
2 groups according to alcohol consumption during the past year (yes = 916, no = 4,268). Data were collected by an<br />
anonymous self-reporting questionnaire which consisted <strong>of</strong> 2 parts: socio-demographic factors and alcohol drinking behavior<br />
during the previous year from December 2007 to February 2008. Descriptive statistics, a chi-square test and multiple logistic<br />
regression were used to analyze the data. Seventeen point seven percent <strong>of</strong> male high school students in this study reported<br />
consuming alcohol. Most <strong>of</strong> the students (73.4%) were 15 years old or younger. Univariate analysis revealed sociodemographic<br />
factors, such as age, educational level, residence, cohabitants, grade point average (GPA), having a job earning<br />
money and having family members with alcohol/drug problems were significantly associated with alcohol consumption (p<br />
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Document Type : Article<br />
Keywords : Migraine; Mri; Vascular<br />
<strong>Abstract</strong> : The objective <strong>of</strong> the present study was to demonstrate MRI evidence <strong>of</strong> vascular compromise as seen in a 17-year- old<br />
female presenting with migrainous headache. The patient had been experiencing migrainous headache 2 days ago. She had<br />
visual auras lasting for a few hours before the attack. Upon meeting with her physician she indicated symptoms <strong>of</strong> left sided<br />
numbness during the headache. No neurological deficit was detected when the first MRI was performed, and all <strong>of</strong> her<br />
symptoms resolved within 4 days <strong>of</strong> the attack. The MRI study showed a focal area <strong>of</strong> restricted diffusion at the right visual<br />
cortex. A short segment <strong>of</strong> vascular enhancement was noted on the surface <strong>of</strong> the affected gyrus. MRS showed a normal Nacetyl<br />
aspartate, choline, and creatine with no elevation <strong>of</strong> lactate. A follow-up MRI study 5 months later showed normal<br />
finding with no residual lesion. The authors concluded that the abnormality on the first MRI was the ischemic insult <strong>of</strong> the<br />
ictal visual cortex which was transient during the migraine attack. The pathophysiology was more likely from reversible focal<br />
venous congestion.<br />
No.212<br />
Author (s) : Chawalparit, O.; Witthiwej, T.; Itthimethin, P.; Sangruchi, T.; Tanboon, J.; Siritho, S.; Prayoonwiwat, N.<br />
Title : Atypical advanced MRI in tumifactive MS: Three case reports<br />
Source : MULTIPLE SCLEROSIS, Volume: 16, Issue: 10, Pages: 1296-1296, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.213<br />
Author (s) : Chawalparit, O.<br />
Title : MRI <strong>of</strong> Multiple Sclerosis in Thailand<br />
Source : MULTIPLE SCLEROSIS, Volume: 16, Issue: 2, Pages: 256-256, Published: FEB <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.214<br />
Author (s) : Chawanpaiboon, S., Cheunwattana, P.<br />
Title : Reference centile chart for fetal nuchal translucency, maternal serum PAPP-A and free beta hCG<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, 93 (2), pp. 154-160.<br />
Document Type : Article<br />
Keywords : Free beta-hCG; Nuchal translucency; PAPP-A; Pregnancy associated plasma protein-A; Reference centile chart; Serum free<br />
beta human chorionic gonadotropin<br />
<strong>Abstract</strong> : Objective: To create reference centile chart <strong>of</strong> fetal nuchal translucency, maternal serum pregnancy associated plasma<br />
protein-A (PAPP-A) and maternal serum free beta human chorionic gonadotropin (beta-hCG) in order to predict preliminarily<br />
Down syndrome in Thai fetuses during 10-14 weeks <strong>of</strong> gestation. Material and Method: This was a prospective, descriptive<br />
cohort study. From 1 January 2004 to 31 December, 2006, a total <strong>of</strong> 1,000 pregnant women during 10-14 weeks <strong>of</strong> gestation<br />
were participated in the present study. Pregnancy outcomes were reviewed from the records. The excluded cases were<br />
chromosomal and major structural abnormalities, twin pregnancy and cases resulting in miscarriage or intrauterine death. All<br />
women had a scan for nuchal translucency (NT) and had blood taken for measurement <strong>of</strong> maternal serum PAPP-A and free<br />
beta-hCG level. Results: The mean NT was 1.6 ± 0.8 mm (range 0.3-14 mm). The 5 th, 50th and 95th centile <strong>of</strong> PAPP-A and<br />
free beta-hCG during 11-14 weeks <strong>of</strong> gestation were 1.54-69, 14-28, 51-57 and 24.8-17, 78-47, 181.6-126.5 mIU/ mL,<br />
respectively. The distribution and the 5% and 95%, lower and upper limits <strong>of</strong> NT, PAPP-A and free betah-CG was presented.<br />
Conclusion: The present study shows that NT measurements increase with increasing gestational age. The mean serum PAPP-<br />
A rises and the mean serum-free beta hCG decreases from 10 to 14 weeks <strong>of</strong> gestation in normal Thai fetuses. These results<br />
can be used for reference value to predict fetal Down Syndrome.<br />
No.215<br />
Author (s) : Chawanpaiboon, S., Kanjana, P.B.N.<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Bacterial vaginosis in threatened preterm, preterm and term labour<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 12, December <strong>2010</strong>, Pages 1351-1355<br />
Document Type : Article<br />
Keywords : Bacterial vaginosis; Preterm labor; Term labor; Threatened preterm labor<br />
<strong>Abstract</strong> : Objective: To present the prevalence <strong>of</strong> bacterial vaginosis in threatened preterm, preterm, and term labor and results after<br />
treatment. Material and Method: Forty-four, 50, and 56 pregnant women with threatened preterm, preterm, and term labor,<br />
respectively were participated. Bacterial vaginosis was diagnosed by Amsel's criteria. Treatment by metronidazole or<br />
clindamycin was used. A case record form recorded maternal age, obstetric history, gestational age at admission and delivery,<br />
examination data, the route <strong>of</strong> delivery, and the newborn birth weight and conditions. Results: The patients in threatened<br />
preterm labor group had significantly positive bacterial vaginosis when compared to those in the term labor group.<br />
Conclusion: Prevalence <strong>of</strong> bacterial vaginosis in threatened preterm, preterm, and term labor were presented. The prevalence<br />
<strong>of</strong> bacterial vaginosis in both preterm labor groups was higher than in the term labor group.<br />
No.216<br />
Author (s) : Chayarop, K.; Temsiririrkkul, R.; Peungvicha, P.; Wongkrajang, Y.; Chuakul, W.; Amnuoypol, S.<br />
Title : Anti-diabetic activity <strong>of</strong> Pseuderanthemum palatiferum Radlk. leaf aqueous extract<br />
Source : PLANTA MEDICA, Volume: 76, Issue: 12, Pages: 1275-1275, Published: AUG <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.217<br />
Author (s) : Cheah, P.Y., Lwin, K.M., Phaiphun, L., Maelankiri, L., Parker, M., Day, N.P., White, N.J., Nosten, F.<br />
Title : Community engagement on the Thai-Burmese border: rationale, experience and lessons learnt<br />
Source : <strong>International</strong> Health, Volume 2, Issue 2, June <strong>2010</strong>, Pages 123-129<br />
Document Type : Article<br />
Keywords : Border population; Community; Community advisory board; Community engagement; Ethics; Migrants<br />
<strong>Abstract</strong> : Community engagement is increasingly promoted in developing countries, especially in international health research, but<br />
there is little published experience. The Shoklo Malaria Research Unit (SMRU) conducts research with refugees, migrant<br />
workers, displaced people, and day migrants on the Thai-Burmese border, and has recently facilitated the set up <strong>of</strong> the Tak<br />
Province Border Community Ethics Advisory Board (T-CAB). Valuable lessons have been learnt from consultation with the T-<br />
CAB especially in the area <strong>of</strong> participant recruitment and the informed consent process. A lot <strong>of</strong> new research questions have<br />
emerged from consultation with the T-CAB. This paper describes our experience, lessons learnt and the unique challenges<br />
faced working with the T-CAB from its initial conception to date. We conclude that consultation with the T-CAB has made<br />
improvements in our research in particular operational and ethical aspects <strong>of</strong> our studies. © <strong>2010</strong> Royal Society <strong>of</strong> Tropical<br />
Medicine and Hygiene.<br />
No.218<br />
Author (s) : Cheamvarasart, K., Supapongpichate, R.<br />
Title : The development <strong>of</strong> potentials on knowledge management for healthy city management <strong>of</strong> local government organizations<br />
Source : Kasetsart Journal - Social Sciences, Volume 31, Issue 1, January <strong>2010</strong>, Pages 40-52<br />
Document Type : Article<br />
Keywords : Healthy city; Knowledge management; Local government organization<br />
<strong>Abstract</strong> : This article presents the research results <strong>of</strong> the development <strong>of</strong> potential on knowledge management for healthy city<br />
management <strong>of</strong> Local Government Organization, using mixed methodology with qualitative and quantitative research. For this<br />
research, the researcher developed and used the curriculum to train 45 <strong>of</strong>ficers performing duty on healthy city management<br />
in 15 Local Government Organizations in Nakom Pathom Province. The results <strong>of</strong> developing potential on knowledge<br />
management for healthy city management <strong>of</strong> Local Government Organization were as follows: The training participants had<br />
more knowledge and better attitude towards knowledge management for healthy city development. The training participants<br />
could suitably apply knowledge and skills on knowledge management for healthy city management in the Local Government<br />
Organizations. The important issue found in this research is that the Local Government Organization is the state agency which<br />
has important role in developing the country and has closest relation with community. Therefore, it is necessary for the<br />
79
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
organization to use various knowledge and develop its potential at all times. However, continuous development in any<br />
aspect <strong>of</strong> the Local Government Organization must be clearly specified in the agency policy or plan.<br />
No.219<br />
Author (s) : Chearskul, S., Pummoung, S., Vongsaiyat, S., Janyachailert, P., Phattharayuttawat, S.<br />
Title : Thai version <strong>of</strong> Three-Factor Eating Questionnaire<br />
Source : Appetite, Volume 54, Issue 2, April <strong>2010</strong>, Pages 410-413<br />
Document Type : Article<br />
Keywords : Diet; Eating behaviors; Exercise; Self-esteem; Stress; TFEQ<br />
<strong>Abstract</strong> : The English version <strong>of</strong> Three-Factor Eating Questionnaire (TFEQ) was translated into the Thai language. The TFEQ Thai version<br />
shows good internal consistency and test-retest reliabilities. Restraint and disinhibition scores, which were higher in females<br />
than males were correlated with body fat but did not relate to BMI. Disinhibition varied positively with restraint and hunger<br />
while restraint was negatively associated with hunger. Lowest restraint was shown in the group that reported liking<br />
carbohydrate, which is a major daily macronutrient among Thai populations. TFEQ Thai version is valid for future study <strong>of</strong><br />
eating behaviors associated with health problems and diseases. © <strong>2010</strong> Elsevier Ltd. All rights reserved.<br />
No.220<br />
Author (s) : Cheausuwantavee, T., Nookaew, S., Cheausuwantavee, C.<br />
Title : Research on disability in Thailand: Meta-analysis and qualitative analysis<br />
Source : <strong>International</strong> Journal <strong>of</strong> Interdisciplinary Social Sciences, Volume 5, Issue 1, <strong>2010</strong>, Pages 311-321<br />
Document Type : Article<br />
Keywords : Disability; Meta-analysis; Rehabilitation; Research synthesis<br />
<strong>Abstract</strong> : This study aimed to synthesize the researches regarding disabilities in Thailand in which had been done during 1997-2009 and<br />
then those were analyzed by meta-analysis and content analysis. The target researches were collected both electronic<br />
database and hard documents that were available in the main 7 libraries <strong>of</strong> public universities in Thailand. The 264<br />
researches meeting inclusion criteria were undertaken and analyzed by content analysis. While, there were only 109 articles<br />
<strong>of</strong> total researches meeting criteria were analyzed by meta-analysis. The results showed that, in terms <strong>of</strong> the content analysis,<br />
the majority <strong>of</strong> researches were unpublished thesis produced during 2001-2006, quantitative studies with descriptive statistics,<br />
educational and social focus, conducted with small sample size and non-probability sampling without statistical calculation.<br />
The principal researchers were usually lecturers. The validity and reliability <strong>of</strong> instruments were usually measured by<br />
specialist and non-specified method respectively. In addition, in terms <strong>of</strong> meta-analysis, the correlation coefficient (r) <strong>of</strong> 109<br />
researches was small (0.22). Multiple regression analysis indicated that the particular variables <strong>of</strong> study features accounted for<br />
46 percent <strong>of</strong> variation in their correlation coefficients. The best predictors were consisted <strong>of</strong> 9 variables including 1)<br />
experimental or quasi-research design(0.31), 2)independent variable as intelligence(0.22), 3) dependent variable as education<br />
(0.16), 4) reliability measurement <strong>of</strong> instrument as Conbach's alpha(0.13), 5) independent variable as socio-economy and<br />
environment (0.07), 6) master degree thesis (0.06), 7) validity measurement <strong>of</strong> instrument as specialist consensus(0.06), 8)<br />
descriptive research design(0.04), and 9) year <strong>of</strong> research completion (0.02) respectively. These research findings suggest that<br />
according to most variables studied have been low relationship and ineffectively selected, thus rigorous researches on<br />
disabilities in Thailand need to be done for identifying the further predictable variables. Additionally, some variables that<br />
have large correlation coefficients such as teaching and learning model also need to be applied for persons with disabilities in<br />
actual settings. © Common Ground, Tavee Cheausuwantavee, Sripair Nookaew, Chommanad Cheausuwantavee, All Rights<br />
Reserved.<br />
No.221<br />
Author (s) : Cheeveewattanagul, N., Jirasripongpun, K., Jirakanjanakit, N., Wattanakaroon, W.<br />
Title : Carrier design for astaxanthin delivery<br />
Source : Advanced Materials Research, Volume 93-94, <strong>2010</strong>, Pages 202-205<br />
Document Type : Conference Paper<br />
Keywords : Antioxidant; Carrier design; Delivery<br />
<strong>Abstract</strong> : Astaxanthin is a natural carotenoid pigment with powerful antioxidant capacity which has been reported for beneficial effects<br />
on human health to prevent cancers and anti-degenerative diseases. Bioavailability <strong>of</strong> astaxanthin is however limited due to<br />
insoluble and instable properties <strong>of</strong> its lipophilic nature. This study describes the cytotoxicity and astaxanthin delivery using a<br />
80
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
surfactant (tween 80) and penetration enhancers (methyl β-cyclodextrin and liposome) to enhance astaxanthin bioavailability<br />
in human hepatocellular carcinoma cell line. Toxicity <strong>of</strong> tween 80 and methyl β-cyclodextrin increased with concentration.<br />
Liposome did not alter cell viability relative to untreated controls, regardless <strong>of</strong> concentration tested. Cellular uptake among<br />
delivery vehicles was compared at 24 hours and was found to be higher than that <strong>of</strong> astaxanthin without a vehicle. The<br />
cellular uptake rate was highest when liposome was used as the delivery vehicle. This finding aids in the development <strong>of</strong><br />
design criteria for vehicles for delivery <strong>of</strong> antioxidants to cells. © (<strong>2010</strong>) Trans Tech <strong>Publications</strong>.<br />
No.222<br />
Author (s) : Chen, Z.-Y., Wang, L., Asavaritkrai, P., Noguchi, C.T.<br />
Title : Up-regulation <strong>of</strong> erythropoietin receptor by nitric oxide mediates hypoxia preconditioning<br />
Source : Journal <strong>of</strong> Neuroscience Research, Volume 88, Issue 14, 1 November <strong>2010</strong>, Pages 3180-3188<br />
Document Type : Article<br />
Keywords : Erythropoietin; Hypoxia; Neuroprotection; Nitric oxide; Receptor expression<br />
<strong>Abstract</strong> : Erythropoietin (Epo), known to stimulate erythroid progenitor cell survival, proliferation, and differentiation, has been shown<br />
to be neuroprotective against brain ischemia in animal models. Both Epo and Epo receptor (EpoR) are expressed in the brain<br />
and are up-regulated by hypoxia. Brain Epo signaling can stimulate neural cell survival and prevent neuron apoptosis.<br />
Neurons from EpoR null mice exhibit marked increased sensitivity to hypoxia. In endothelial cells, Epo has been shown to<br />
stimulate nitric oxide (NO) production, particularly at low pO2. We found here that the EpoR expression on neural cells and<br />
Epo's neuroprotective effect were regulated by NO. Hypoxia increased NO production as well as EpoR expression, and<br />
inhibition <strong>of</strong> NOS activity reduced the proportion <strong>of</strong> EpoR-expressing neurons induced at low pO2. Conversely, addition <strong>of</strong> NO<br />
donor to cultures grown under normoxia induced EpoR. Similarly, NO donor increased EpoR promoter activity in a reporter<br />
gene assay, suggesting that NO regulates EpoR at the transcription level. Preincubation <strong>of</strong> neurons with NO results in induction<br />
<strong>of</strong> EpoR, which gives rise to protection against hypoxia even in the absence <strong>of</strong> exogenous Epo, although at high concentration<br />
NO is toxic. These data provide evidence <strong>of</strong> a role for NO in Epo activity in brain and suggest links between NO production,<br />
EpoR expression, and Epo signaling in neuroprotection. Published <strong>2010</strong> Wiley-Liss, Inc.<br />
No.223<br />
Author (s) : Chen, S. A.; Hiley, C.; Petusksiri, J.; Andic, F.; Riesterer, O.; Torres, M.<br />
Title : Influence <strong>of</strong> Breast Reconstruction on Postmastectomy Radiotherapy: Global Perceptions and Practice Patterns<br />
Source : INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS, Volume: 78, Issue: 3, Supplement: S, Pages: S235-<br />
S236, Published: <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.224<br />
Author (s) : Chenprakhon, P., Sucharitakul, J., Panijpan, B., Chaiyen, P.<br />
Title : Measuring binding affinity <strong>of</strong> protein-ligand interaction using spectrophotometry: Binding <strong>of</strong> neutral red to rib<strong>of</strong>lavin-binding<br />
protein<br />
Source : Journal <strong>of</strong> Chemical Education, Volume 87, Issue 8, <strong>2010</strong>, Pages 829-831<br />
Document Type : Article<br />
Keywords : Bioanalytical Chemistry; Biochemistry; Hands-On Learning/Manipulatives; Laboratory instruction; Noncovalent<br />
interactions; Physical Chemistry; Proteins/peptides; Titration/volumetric analysis; Upper-division undergraduate; UV-Vis<br />
spectroscopy<br />
<strong>Abstract</strong> : The dissociation constant, Kd, <strong>of</strong> the binding <strong>of</strong> rib<strong>of</strong>lavin-binding protein (RP) with neutral red (NR) can be determined by<br />
titrating RP to a fixed concentration <strong>of</strong> NR. Upon adding RP to the NR solution, the maximum absorption peak <strong>of</strong> NR shifts to<br />
545 nm from 450 nm for the free NR. The change <strong>of</strong> the absorption can be used to determine the Kd by plotting an<br />
absorbance ratio versus the concentration <strong>of</strong> free RP. The data are analyzed via a nonlinear fitting analysis to yield a Kd <strong>of</strong> 2.2<br />
± 0.3 × 10-6 mol L-1. This experiment illustrates how the Kd value can be determined and used to evaluate the strength <strong>of</strong><br />
protein-ligand interactions. This experiment is appropriate for undergraduate students or first-year graduate students in<br />
biochemistry, chemistry, or physical chemistry. © <strong>2010</strong> The American Chemical Society and Division <strong>of</strong> Chemical Education,<br />
Inc.<br />
81
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.225<br />
Author (s) : Cherdshewasart, W., Mahapanichkul, T., Boonchird, C.<br />
Title : Estrogenic and anti-estrogenic activities <strong>of</strong> the Thai traditional herb, Butea superba Roxb.<br />
Source : Bioscience, Biotechnology and Biochemistry, Volume 74, Issue 11, <strong>2010</strong>, Pages 2176-2182<br />
Document Type : Article<br />
Keywords : Antiestrogenic; Butea superba; Estrogen receptor; Phytoestrogen; Yeast estrogen screening<br />
<strong>Abstract</strong> : This study evaluated the estrogenic and antiestrogenic activities <strong>of</strong> native and in vitro hepatic metabolized tuberous extracts<br />
<strong>of</strong> wild Butea superba collected from 23 out <strong>of</strong> the 76 provinces in Thailand by yeast estrogen screening (YES). The YES<br />
screen used consisted <strong>of</strong> the human estrogen receptors hERa and hERβ and the human transcriptional intermediary factor 2<br />
or human steroid receptor coactivator 1, respectively, together with the β-galactosidase expression cassette as the reporter.<br />
The relative potency, effectiveness and relative inductive efficiency were evaluated by determining the β-galactosidase<br />
activity (EC50) <strong>of</strong> each tuberous extract in relation to that induced by 17β-estradiol. Six pure compounds isolated from B.<br />
superba were tested in parallel and exhibited a maximum relative potency compared to 17β-estradiol <strong>of</strong> 15.5% and 5.27% in<br />
the respective hERa and hERβ assays. Eighteen and seventeen plant extracts were respectively found to interact with the<br />
hERa and hERβ receptors in the YES assays with higher relative potency and relative inductive efficiency with hERβ than with<br />
hERa. The selected plant extracts tested exhibited antiestrogenic activity. Coincubation with the rat liver S9 mixture also<br />
elevated the estrogenic potency <strong>of</strong> these plant extracts.<br />
No.226<br />
Author (s) : Cherdshewasart, W., Sutjit, W., Pulcharoen, K., Chulasiri, M.<br />
Title : Mutagenic and antimutagenic effects <strong>of</strong> the traditional herb used for treating erectile dysfunction, Butea superba Roxb.<br />
Source : Bioscience, Biotechnology and Biochemistry, Volume 74, Issue 5, <strong>2010</strong>, Pages 923-927<br />
Document Type : Article<br />
Keywords : Ames test; Antimutagenic effect; Butea superba; Flavonoid; Micronucleus test<br />
<strong>Abstract</strong> : Butea superba is a traditional tuberous Thai plant enriched with flavonoids that is used for treating erectile dysfunction. We<br />
investigated the mutagenic and antimutagenic potentials <strong>of</strong> a B. superba extract by using the pre-incubation method <strong>of</strong> the<br />
Ames test. Salmonella typhimurium strains TA 98 and TA 100 were applied as the tester strains in the presence and absence<br />
<strong>of</strong> an S9 mixture. Prior to the mutagenic and antimutagenic tests, the survival <strong>of</strong> the tester strains was measured by treating<br />
with the B. superba extract. The results show that the B. superba extract exhibited dose-dependent cytotoxic effects. Data<br />
from the Ames test revealed that the B. superba extract to be non-mutagenic in the presence and absence <strong>of</strong> the S9 mixture.<br />
In contrast, the B. superba extract showed antimutagenic potential towards either or both <strong>of</strong> the tested mutagens: 2-(2-furyl)-<br />
3-(5-nitro-2-furyl)-acrylamide (AF-2) and benzo(a)pyrene (B(a)P) in the respective presence and absence <strong>of</strong> the S9 mixture,<br />
respectively. The plant antimutagenic activity was confirmed by a rec assay. A further study by micronucleus test<br />
demonstrated that the B. superba extract at the maximum loading volume could induce acute micronucleus formation in the<br />
tested animals. The in vitro mutagenic and antimutagenic assays confirmed the safe consumption <strong>of</strong> B. superba products at<br />
low dose (not more than 781.25μg/ml <strong>of</strong> the plant extract), but the in vivo genotoxic assay demonstrated the unsafe<br />
consumption at a high dose (300 mg/kg <strong>of</strong> the BW plant extract or 16 g/kg <strong>of</strong> the BW plant powder).<br />
No.227<br />
Author (s) : Chetsawang, B., Chetsawang, J., Govitrapong, P.<br />
Title : Hydrogen peroxide toxicity induces Ras signaling in human neuroblastoma SH-SY5Y cultured cells<br />
Source : Journal <strong>of</strong> Biomedicine and Biotechnology, Volume <strong>2010</strong>, <strong>2010</strong>, Article number 803815<br />
Document Type : Article<br />
Keywords : guanosine 5' o (2 thiodiphosphate), hydrogen peroxide, n [[5 [(2 amino 3 mercaptopropyl)amino][1,1' biphenyl] 2<br />
yl]carbonyl]methionine methyl ester, Ras protein; article, cell culture, cell viability, cytotoxicity, dopaminergic nerve<br />
cell, human, human cell, nerve cell degeneration, neuroblastoma cell; Animals, Cell Line, Tumor, Cell Survival, Dose-<br />
Response Relationship, Drug, Humans, Hydrogen Peroxide, Neuroblastoma, ras Proteins, Signal Transduction<br />
<strong>Abstract</strong> : It has been reported that overproduction <strong>of</strong> reactive oxygen species occurs after brain injury and mediates neuronal cells<br />
degeneration. In the present study, we examined the role <strong>of</strong> Ras signaling on hydrogen peroxide-induced neuronal cells<br />
degeneration in dopaminergic neuroblastoma SH-SY5Y cells. Hydrogen peroxide significantly reduced cell viability in SH-SY5Y<br />
cultured cells. An inhibitor <strong>of</strong> the enzyme that catalyzes the farnesylation <strong>of</strong> Ras proteins, FTI-277, and a competitive inhibitor<br />
82
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>of</strong> GTP-binding proteins, GDP-beta-S significantly decreased hydrogen peroxide-induced reduction in cell viability in SH-SY5Y<br />
cultured cells. The results <strong>of</strong> this study might indicate that a Ras-dependent signaling pathway plays a role in hydrogen<br />
peroxide-induced toxicity in neuronal cells. Copyright <strong>2010</strong> Jirapa Chetsawang et al.<br />
No.228<br />
Author (s) : Chetsawang, B.; Parameyong, A.; Suwanjang, W.; Govitrapong, P.<br />
Title : METHAMPHETAMINE INDUCES TOXICITY AND ALTERATION IN MITOCHONDRIAL FISSION PROTEIN LEVELS IN NEUROBLASTOMA<br />
SH-SY-5Y CULTURED CELLS<br />
Source : JOURNAL OF NEUROCHEMISTRY, Volume: 115, Supplement: 1, Pages: 81-81, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.229<br />
Author (s) : Cheunsuchon, B.; Incharoen, P.; Chanchairujira, T.; Shayakul, C.<br />
Title : Macrophage in Lupus Nephritis: Can It Be a Predictor <strong>of</strong> Treatment Response?<br />
Source : MODERN PATHOLOGY, Volume: 23, Supplement: 1, Pages: 338A-338A, Meeting <strong>Abstract</strong>: 1508, Published: FEB <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.230<br />
Author (s) : Chiaravutthi, Y.<br />
Title : Brand valuation <strong>of</strong> ICT products: The case <strong>of</strong> Thailand<br />
Source : Asia-Pacific Journal <strong>of</strong> Business Administration, Volume 2, Issue 2, <strong>2010</strong>, Pages 185-202<br />
Document Type : Article<br />
Keywords : Brand equity; Communication technologies; Prices; Thailand<br />
<strong>Abstract</strong> : Purpose The paper aims to adopt the hedonic price approach to quantify the brand equity <strong>of</strong> information and<br />
communication technology (ICT) products, narrowed down to laptop computers, laser printers, liquid crystal display<br />
computer screens, and mobile phones. Design/methodology/approach The hedonic price model features the list price as the<br />
dependent variable <strong>of</strong> the regression, whilst the measurable attributes <strong>of</strong> the product and brand dummies are on the righthand<br />
side. Additionally, the model can be adjusted to measure brand effects on pr<strong>of</strong>it margins as well. Findings In most <strong>of</strong><br />
the price and log price models, brand dummies are significant, and positively linked to the consumers' willingness to pay.<br />
Nevertheless, amongst the four ICT products in this study, only the laptop brands show positive values. Negative but<br />
significant brand dummies suggest that brands are undoubtedly important; however, other features exhibit higher value to<br />
consumers. Research limitations/implications As is the case with other financial approaches to valuing brands, the results do<br />
not explain how to exploit those values; rather, it identifies the brand's position as measured against other brands. Practical<br />
implications Negative brand premiums imply that brand loyalty is not strong in the market, and that the opportunity exists for<br />
a new brand's penetration. The construction <strong>of</strong> brand premium rankings should prove beneficial to firms who wish to<br />
evaluate their current position against other competitors. Regarding the products' features, the results suggest that consumers<br />
generally focus their decision to purchase a particular brand on its basic or core features. Originality/value The paper<br />
proposes another approach to assessing brand equity, namely, in terms <strong>of</strong> both price and pr<strong>of</strong>it margin premiums. Though<br />
imperfect, the hedonic methodology is relatively simple and relies on available secondary data. © <strong>2010</strong> Emerald Group<br />
Publishing Limited. All rights reserved.<br />
No.231<br />
Author (s) : Chierakul, N., Danchaivijitr, S., Kontee, P., Naruman, C.<br />
Title : Reliability and validity <strong>of</strong> long case and short case in internal medicine board certification examination<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 4, April <strong>2010</strong>, Pages 424-428<br />
Document Type : Article<br />
Keywords : Board certifying examination; Internal medicine; Long case; Short case<br />
83
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : Objective: To be certified for the Thai Board <strong>of</strong> Internal Medicine, each candidate must pass both written and clinical<br />
examinations performed in different academic years. The present study aimed to assess the reliability and validity <strong>of</strong> the long<br />
case and short case which contribute major fractions in the clinical part <strong>of</strong> board certifying examination. Material and Method:<br />
Data from 585 internal medicine residents entering a first-attempt clinical part in board certifying examination during the<br />
academic year 2005-2007 were collected. Inter-rater reliability and construct validity <strong>of</strong> the long case and short case were<br />
then examined. Results: Good to excellent intraclass correlation (ICC) <strong>of</strong> scores from different examiners was demonstrated<br />
(ICC between 0.71 and 0.97) and the variation ranged from 15.3 to 27.3%. For different occasions <strong>of</strong> examination, class<br />
normalized gain was between -0.7 and -9.0% and negative individual normalized gain was observed in 45.6% to 48.2% <strong>of</strong> the<br />
candidates. Conclusion: Acceptable inter-rater reliability was demonstrated in long case and short case in clinical examination<br />
for the Thai Board <strong>of</strong> Internal Medicine. But construct validity for this type <strong>of</strong> clinical assessment was not established.<br />
No.232<br />
Author (s) : Chiewvit, P., Tritakarn, S.-O., Nanta-aree, S., Suthipongchai, S.<br />
Title : Degree <strong>of</strong> midline shift from CT scan predicted outcome in patients with head injuries<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 1, January <strong>2010</strong>, Pages 99-107<br />
Document Type : Article<br />
Keywords : CT scan; Degree <strong>of</strong> midline shifted; Glasglow coma score; Head injury<br />
<strong>Abstract</strong> : Objective: To evaluate the relationship between the degree <strong>of</strong> midline shift by Computed Tomography (CT) finding and<br />
Glasglow Coma Score (GCS) as a predictive <strong>of</strong> clinical outcome in patients after head injury. Method and Method: The present<br />
study was performed by retrospectively reviewing 216 consecutive cases <strong>of</strong> traumatic head injury admitted to the trauma<br />
center in Siriraj Hospital from 1999 until 2004. All patients were evaluated for level <strong>of</strong> consciousness by a neurosurgeon<br />
determining by GCS and underwent CT brain for evaluation <strong>of</strong> intracranial hemorrhage and midline shift. The final clinical<br />
outcome was also divided into two groups; good outcome for the patients who recovered well with moderate disability and<br />
the poor outcome for the patients who suffered severe disability, vegetative status and death. Then, the authors compared<br />
midline shift vs. GCS and midline shift vs. clinical outcomes. Results: Total <strong>of</strong> 216 cases, the three most common types <strong>of</strong><br />
head injury were motorcycle accident, fall or assault and car accident. 96 <strong>of</strong> 216 patients had midline shifting, 53 <strong>of</strong> 96<br />
patients had CT scan <strong>of</strong> midline shifting less than 10 mm whereas 37 <strong>of</strong> 96 patients had a CT scan <strong>of</strong> greater than 10 mm <strong>of</strong><br />
midline shifting. 63.3% with midline shifting up to 10 mm had severe head injury and up to 81% with brain shifting greater<br />
than 10 mm had severe head injury. The clinical outcome also showed that poor clinical outcomes correlated to midline<br />
shifting greater than 10 mm. Conclusion: The increased degree <strong>of</strong> midline shift in patients with head injuries by CT scan was<br />
related to the severity <strong>of</strong> head injury (GCS = 3-12) and was significantly related to poor final clinical outcome.<br />
No.233<br />
Author (s) : Chiewvit, S., Phasuk, S., Surapako, S., Juiklom, W., Chiewvit, P.<br />
Title : Screening may not be accurate word to represent the cases submitted to PET/CT evaluation for primary tumor in a patient<br />
who has abnormal serum tumor marker<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 3, March <strong>2010</strong>, Pages 330-336<br />
Document Type : Review<br />
Keywords : Rising tumor marker; Screening 18F FDG PET/CT<br />
<strong>Abstract</strong> : Objective: Determine the value <strong>of</strong> PET/CT in unknown primary cancer patient with high tumor marker and negative study for<br />
clinical and conventional imaging. Material and Method: A retrospective database review <strong>of</strong> 417 patients who received PET/CT<br />
between July 2006 and August 2007 in National cyclotron and PET center at Chulabhorn cancer center was done. Patients<br />
were included in this study if the diagnosis were unknown primary cancer and rising tumor marker. Twelve patients were<br />
included in this study. Data included age, gender, tumor marker rising, anatomical imaging finding (CT and MRI), PET finding<br />
and clinical follow-up. Results: Nine cases had normal PET/CT. This showed that PET/CT does not get more information than<br />
conventional imaging. The PET scan showed positive in three cases, #5, #6 and #10. Two cases were false positive, #5 and #6.<br />
Case #5 had clinical follow-up for one year and revealed to be normal. Case #6 PET showed markedly glucose avid lesion at<br />
tumor thrombus but contrast CT confirm blood clot and the patient was treat with wafarin and claxane. The follow-up<br />
clinical showed improvement. The high serum CA 125 explained by lung infarction caused the false positive. In case#10, the<br />
PET/CT suggested lung cancer at basal segment <strong>of</strong> LLL. Conclusion: Screening 18F FDG PET/CT is not appropriate in unknown<br />
primary with rising tumor marker and normal conventional imaging is required.<br />
84
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.234<br />
Author (s) : Chimsuntorn, S., Sungkanuparph, S., Manosuthi, W.<br />
Title : Successful viral suppression with subsequent efavirenz-based regimen in HIV-1-infected patients who stop nevirapine prior to<br />
discontinuation <strong>of</strong> the NRTI backbone<br />
Source : Journal <strong>of</strong> the <strong>International</strong> Association <strong>of</strong> Physicians in AIDS Care, Volume 9, Issue 1, <strong>2010</strong>, Pages 43-45<br />
Document Type : Article<br />
Keywords : Allergy; Efavirenz; Efficacy; Nevirapine; Thailand<br />
<strong>Abstract</strong> : Objective: To study antiviral response <strong>of</strong> efavirenz (EFV)-based antiretroviral therapy (ART) in HIV-1-infected patients who had<br />
previously discontinued nevirapine (NVP) and continued the nucleoside reverse transcriptase inhibitor (NRTI) backbone for 7<br />
to 14 days after stopping NVP. Methods: A retrospective cohort study was conducted in patients who discontinued NVP. CD4<br />
count and plasma HIV-1 RNA levels were monitored through 48 weeks <strong>of</strong> EFV-based regimen. Results: Forty-five patients were<br />
eligible with a mean ± SD age <strong>of</strong> 37 ± 11 years; 60% were males. Median (interquartile range [IQR]) baseline CD4 count was 43<br />
(19-150) cells/mm3 and median (IQR) plasma HIV-1 RNA was 5.7 (5.3-5.9) log copies/mL. Median (IQR) duration from stopping<br />
NVP to initiating EFV was 13 (7-18) days. At 48 week, 33 (73.3%) <strong>of</strong> 45 patients achieved plasma HIV-1 RNA <50 copies/mL.<br />
At week 12, 24, and 48, median CD4 counts were 193, 238, and 268 cells/mm3, respectively (P <.05). No studied risk factor<br />
was associated with achieving HIV-1 RNA <50 copies/mL at 48 weeks (P >.05). Conclusion: The strategy <strong>of</strong> extended short<br />
half-life NRTIs in the regimen after discontinuation <strong>of</strong> NVP is justified. © The Author(s) <strong>2010</strong>.<br />
No.235<br />
Author (s) : Ching, L.Y., Möller, K., Suthakorn, J.<br />
Title : Non-radiological colonoscope tracking: Image guided colonoscopy using commercially available electromagnetic tracking<br />
system<br />
Source : <strong>2010</strong> IEEE Conference on Robotics, Automation and Mechatronics, RAM <strong>2010</strong>, <strong>2010</strong>, Article number 5513213, Pages 62-67<br />
Document Type : Conference Paper<br />
Keywords : Colonoscope tracking; Conolonoscopy; Electromagnetic tracking; Image guided intervention; Surgical navigation<br />
<strong>Abstract</strong> : A non-radiological method <strong>of</strong> visualizing the path <strong>of</strong> a colonoscope using a chain <strong>of</strong> electromagnetic sensor coils along the<br />
biopsy channel <strong>of</strong> the instrument has been developed. The electromagnetic imaging system has been introduced as an aid to<br />
colonoscopy, and reveals a great potential for assisting endoscopists. There is an existing model available on the market by<br />
Olympus, ScopeGuide; however, due to cost consideration and other factors concerned, some hospitals may not want to<br />
replace existing instruments. This paper discusses about the possibility <strong>of</strong> using a commercially available electromagnetic<br />
tracking system, the Northern Digital Aurora system and presents a simple algorithm employed to find a representation <strong>of</strong> the<br />
colonoscope path. A possibility <strong>of</strong> reducing the amount <strong>of</strong> sensor coils used in existing model is also discussed. The visual<br />
guidance is expected to provide the user with a sense <strong>of</strong> assurance, which is <strong>of</strong>ten missing in the navigation <strong>of</strong> colonoscope.<br />
The work may be useful in locating the exact position when a lesion is found during the procedure, and to identify a loop<br />
when it is formed. It may also be useful as a navigational aid in colonoscopy training and teaching purposes. ©<strong>2010</strong> IEEE.<br />
No.236<br />
Author (s) : Chinsakchai, K., Wongwanit, C., Ruangsetakit, C., Mutirangura, P.<br />
Title : Successful treatment in superior mesenteric artery embolism: A case report and literature review<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 6, June <strong>2010</strong>, Pages 739-744<br />
Document Type : Article<br />
Keywords : Acute intestinal ischemia; Embolectomy; Embolism; Intestinal gangrene; Superior mesenteric artery<br />
<strong>Abstract</strong> : The authors report a successful management <strong>of</strong> acute superior mesenteric artery embolism in a patient during the treatment<br />
<strong>of</strong> popliteal artery embolism. The diagnosis <strong>of</strong> this disease was confirmed by computerized tomographic angiography.<br />
Immediate surgical embolectomy and bowel resection were performed and postoperatively, he made an uneventful recovery<br />
except for minimal watery diarrhea for one week. In the present report the authors also review the in- patient records at<br />
Siriraj Hospital during 2005-2009 consisting <strong>of</strong> 14 cases with the claim diagnosis <strong>of</strong> this condition. Most <strong>of</strong> the patients<br />
developed peritonism on abdominal examinations showing a delay in diagnosis. The mortality rate was 86% (12 cases) which<br />
was higher than international reports. It is important to note that patients presenting with abdominal pain with underlying risk<br />
factors <strong>of</strong> arterial embolism is the clue in early diagnosis <strong>of</strong> this condition. CTA mesenteric artery is the most appropriate<br />
investigation to visualize the presence <strong>of</strong> embolism. Finally, immediate revascularization treatment is essential in the<br />
successful management <strong>of</strong> this fatal vascular problem.<br />
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No.237<br />
Author (s) : Chintrakarn, P., Prasatkitjaroen, P.<br />
Title : Reassessing the determinants <strong>of</strong> international stock market correlation<br />
Source : <strong>International</strong> Research Journal <strong>of</strong> Finance and Economics, Volume 60, December <strong>2010</strong>, Pages 7-14<br />
Document Type : Article<br />
Keywords : Cross-border portfolio investment; Equity flows; Gravity model; Home bias; Information asymmetries<br />
<strong>Abstract</strong> : The purpose <strong>of</strong> this paper is to examine the determinants <strong>of</strong> international stock market correlation by applying the gravity<br />
model where the roles <strong>of</strong> distance and overlapping opening hours are <strong>of</strong> key interest in this study. Based on the work <strong>of</strong><br />
Flavin, Hurley, & Rousseau (2002), we utilize a dynamic gravity model, the findings herein confirm the importance <strong>of</strong><br />
overlapping hour variable as the main determinant <strong>of</strong> international stock markets correlation. © EuroJournals Publishing, Inc.<br />
<strong>2010</strong>.<br />
No.238<br />
Author (s) : Chiowchanwisawakit, P.; Lambert, R.G.W.; Maksymowych, W.P.<br />
Title : FAT INFILTRATION IN SPONDYLOARTHRITIS: AN IMAGING BIOMARKER REFLECTING RESOLUTION OF INFLAMMATION<br />
Source : CLINICAL AND EXPERIMENTAL RHEUMATOLOGY, Volume: 28, Issue: 4, Pages: 636-636, Published: JUL-AUG <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.239<br />
Author (s) : Chirdkiatgumchai, V., Ruangdaraganon, N., Roongpraiwan, R., Sombuntham, T., Rojmahamongkol, P., Prasertchai, R.<br />
Title : Thai parental perception <strong>of</strong> the etiology <strong>of</strong> autism spectrum disorders with an emphasis on genetics<br />
Source : ASIAN BIOMEDICINE, Volume: 4, Issue: 5, Pages: 773-779, Published: OCT <strong>2010</strong><br />
Document Type : Article<br />
Keywords : Autism spectrum disorders; etiology; genetics; multi-factorial; parental perception; Thai<br />
<strong>Abstract</strong> : Background: More than 90% <strong>of</strong> cases <strong>of</strong> autism spectrum disorders (ASDs) are idiopathic and are multi-factorial inheritant in<br />
etiology. Although parental perceptions <strong>of</strong> ASDs etiologies including genetics have been investigated well in western<br />
countries, there is no study regarding Asian parental perspectives on the genetic etiology <strong>of</strong> ASDs. Objectives: Examine what<br />
Thai parents perceived to be the etiology <strong>of</strong> ASDs with particular focus on the role <strong>of</strong> genetics, and analyze the correlation<br />
between demographics <strong>of</strong> parents and the perception <strong>of</strong> genetics as an etiology <strong>of</strong> ASDs. Methods: Children diagnosed with<br />
an ASD at Ramathibodi Hospital were recruited, and 328 questionnaires were mailed to their parents between February 1 and<br />
March 31, 2008. Based on 252 answers received, the perception <strong>of</strong> genetics as an etiology <strong>of</strong> ASDs was evaluated using<br />
univariate and multivariate analysis. Results: Among 252 parents, 44.0% cited genetics, 52% cited other prenatal and perinatal<br />
factors such as stress and chemical exposure during pregnancy, 33% cited postnatal factors such as child rearing, child's<br />
viewing <strong>of</strong> television, and playing computer game as etiologies <strong>of</strong> ASDs. Parents' education, positive family history <strong>of</strong> ASDs,<br />
and positive family history <strong>of</strong> speech delay were all significantly associated with the perception <strong>of</strong> genetics as an etiology <strong>of</strong><br />
ASDs. Conclusions: Less than half <strong>of</strong> Thai parents cited genetics as an etiology <strong>of</strong> ASDs. This finding alerts medical<br />
pr<strong>of</strong>essionals to spend more time and effort to educate and counsel parents.<br />
No.240<br />
Author (s) : Chittasirinuwat, O., Kruatong, T., Paosawatyanyong, B.<br />
Title : College students' intuitive understanding and problem-solving <strong>of</strong> energy and momentum<br />
Source : AIP Conference Proceedings, Volume 1263, <strong>2010</strong>, Pages 79-82<br />
Document Type : Conference Paper<br />
Keywords : Conservation; Energy and Momentum; Gauss gun; Problem-Solving Thinking<br />
<strong>Abstract</strong> : This study addresses students' intuitive understanding <strong>of</strong> energy and momentum and their problem solving ability. The<br />
subjects <strong>of</strong> this research were students who had experiences with conservation <strong>of</strong> energy and momentum. Nine<br />
undergraduate students completed event-based Interviews with three related events which composed <strong>of</strong> Event I: Simple<br />
collisions, Event II: Newton's cradle and Event III: Gauss gun. Their intuitive understanding was explored through three welldefined<br />
items involving Event I and II. The interviews revealed that most students explained the two events by utilizing their<br />
intuitive understanding rather than scientific conceptions. Then problem-solving thinking was identified through ill-defined<br />
86
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
problems involving Event III. From the Gauss gun setting, students were asked to explain how Gauss gun works, how to build<br />
the highest power Gauss gun and interpret the graph <strong>of</strong> mass and distance <strong>of</strong> steel ball after collisions. Research findings<br />
showed that students who have fairly good command <strong>of</strong> basic knowledge, tended to use <strong>of</strong> problem solving strategies as<br />
expected. For example, a student who understood the perfectly transferring energy and momentum <strong>of</strong> the equal mass <strong>of</strong><br />
balls, was able to identify the possible factors for design more effective Gauss gun reasonably. However, most <strong>of</strong> the students<br />
were unable to use suitable vocabulary in providing reasons and explanations for certain problem-solving procedures. Thus,<br />
lacking basic knowledge can impede problem-solving thinking. It is hope that these findings will serve as a reference for<br />
educators in improving the learning and teaching <strong>of</strong> energy and momentum in general and problem solving instruction in<br />
particular. © <strong>2010</strong> American Institute <strong>of</strong> Physics.<br />
No.241<br />
Author (s) : Choetkiertikul, M., Sunetnanta, T.<br />
Title : A risk assessment model for <strong>of</strong>fshoring using CMMI quantitative approach<br />
Source : Proceedings - 5th <strong>International</strong> Conference on S<strong>of</strong>tware Engineering Advances, ICSEA <strong>2010</strong>, <strong>2010</strong>, Article number 5615709,<br />
Pages 331-336<br />
Document Type : Conference Paper<br />
Keywords : Offshoring s<strong>of</strong>tware development; Quantitative CMMI assessment; Risk analysis<br />
<strong>Abstract</strong> : Risk analysis and assessment obviously provides valuable insights to <strong>of</strong>fshoring projects to identify and evaluate the<br />
magnitude <strong>of</strong> risks associated with the activities and the work products being considered. In <strong>of</strong>fshoring s<strong>of</strong>tware industry,<br />
successful execution <strong>of</strong> risk analysis drastically relies on strong s<strong>of</strong>tware process skills and management skills to resolve the<br />
differences in cultures, languages, time zones, and development which are used across distributed project teams. One way to<br />
ease such differences is to provide a model which <strong>of</strong>fers a rational and automated basis for quantifying and monitoring risks<br />
and providing specific decision-making guidance while maintaining the nature <strong>of</strong> <strong>of</strong>fshoring in a distributed manner. This paper<br />
presents an extension <strong>of</strong> our previous model <strong>of</strong> quantitative CMMI assessment. We further apply the best practices from the<br />
Capability Maturity Model Integration (CMMI) as a guideline for quantitative risk analysis in <strong>of</strong>fshoring and using risk taxonomy<br />
from the S<strong>of</strong>tware Engineering Institute (SEI) Taxonomy-Based Risk Identification. This work aims to reduce the process<br />
overhead <strong>of</strong> risk assessment by automatically collecting data from the project management repository to adequately and<br />
appropriately determine the approximate level <strong>of</strong> risk in <strong>of</strong>fshoring projects. © <strong>2010</strong> IEEE.<br />
No.242<br />
Author (s) : Chokchaisiri, R., Chaneiam, N., Svasti, S., Fucharoen, S., Vadolas, J., Suksamrarn, A.<br />
Title : Labdane diterpenes from the aerial parts <strong>of</strong> curcuma comosa enhance fetal hemoglobin production in an erythroid cell line<br />
Source : Journal <strong>of</strong> Natural Products, Volume 73, Issue 4, 23 April <strong>2010</strong>, Pages 724-728<br />
Document Type : Article<br />
Keywords : beta globin, cisplatin, coronarin e, curcucomosin a, curcucomosin b, curcucomosin c, enhanced green fluorescent<br />
protein, hemin, hemoglobin F, heptane derivative, isocoronarin d, labdane diterpene, plant extract, unclassified<br />
drug, villosin, zerumin; article, carbon nuclear magnetic resonance, cell viability, controlled study, Curcuma comosa, Curcuma<br />
longa, cytotoxicity, drug activity, drug isolation, drug structure, erythroid cell, human, human cell, protein expression, proton<br />
nuclear magnetic resonance; Curcuma, Diterpenes, Erythroid Cells, Fetal Hemoglobin, Green Fluorescent<br />
Proteins, Humans, K562 Cells, Molecular Structure, Nuclear Magnetic Resonance, Biomolecular, Thailand; Curcuma comosa<br />
<strong>Abstract</strong> : Three new labdane diterpenes, curcucomosins A-C (1-3), four known labdane diterpenes, 4-7, and a known diarylheptanoid,<br />
8, were isolated from the aerial parts <strong>of</strong> Curcuma comosa. The structures <strong>of</strong> the new diterpenes were elucidated by<br />
spectroscopic data analysis. The fetal hemoglobin (Hb F) induction potency <strong>of</strong> the isolated compounds was examined using a<br />
K562 reporter cell line harboring the enhanced green fluorescence protein (EGFP) gene under the control <strong>of</strong> a Gγ-globin<br />
promoter. Compound 6, isocoronarin D, exhibited the highest Hb F induction effect <strong>of</strong> 1.6-fold at 20 μM. © <strong>2010</strong> The<br />
American Chemical Society and American Society <strong>of</strong> Pharmacognosy.<br />
No.243<br />
Author (s) : Chokephaibulkit, K., Plipat, N., Yoksan, S., Phongsamart, W., Lappra, K., Chearskul, P., Chearskul, S., Wittawatmongkol, O.,<br />
Vanprapar, N.<br />
Title : A comparative study <strong>of</strong> the serological response to Japanese encephalitis vaccine in HIV-infected and uninfected Thai<br />
children<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Source : Vaccine, Volume 28, Issue 20, 30 April <strong>2010</strong>, Pages 3563-3566<br />
Document Type : Article<br />
Keywords : HIV-exposed infants; Immunogenicity; Japanese encephalitis; Safety; Thailand; Vaccine<br />
<strong>Abstract</strong> : We report a prospective study <strong>of</strong> mouse brain derived inactivated Japanese encephalitis (JE) vaccine, given in 3-dose EPI<br />
program to human immune deficiency virus (HIV)-exposed Thai infants. 18 HIV-infected receiving antiretroviral therapy with<br />
median baseline CD4 <strong>of</strong> 33.1%, and 92 HIV-uninfected children were studied. All but one HIV-infected child seroconverted<br />
after the second dose. The geometric mean titers (GMTs) 3 months after the second and third doses in HIV-infected vs HIVuninfected<br />
children were 247 vs 938 (p = 0.022), and 2273 vs 24069 (p = 0.009), respectively. Urticaria or angioedema found in<br />
4% and 6% in HIV-infected and -uninfected children, respectively (p = 1.0). The vaccine was safe and immunogenic but<br />
antibody response in HIV-infected children was not as high as in uninfected children. © <strong>2010</strong> Elsevier Ltd. All rights reserved.<br />
No.244<br />
Author (s) : Chokephaibulkit, K., Sirivichayakul, C., Thisyakorn, U., Sabchareon, A., Pancharoen, C., Bouckenooghe, A., Gailhardou, S., Boaz,<br />
M., Feroldi, E.<br />
Title : Safety and immunogenicity <strong>of</strong> a single administration <strong>of</strong> live-attenuated japanese encephalitis vaccine in previously primed 2-<br />
to 5-year-olds and naive 12- to 24-month-olds: Multicenter randomized controlled trial<br />
Source : Pediatric Infectious Disease Journal, Volume 29, Issue 12, December <strong>2010</strong>, Pages 1111-1117<br />
Document Type : Article<br />
Keywords : attenuated vaccines; hepatitis A vaccines; infant; Japanese encephalitis vaccines; preschool child; vaccination<br />
<strong>Abstract</strong> : Background: Safe and effective Japanese encephalitis (JE) vaccines are needed to protect populations living in or visiting<br />
endemic areas. A live-attenuated JE-chimeric virus vaccine (JE-CV) has been developed with a single-dose regimen. Methods:<br />
In an open-label, crossover study, 100 children aged 2 to 5 years with a history <strong>of</strong> 2-dose primary vaccination with mousebrain<br />
derived inactivated JE vaccine according to the Thai Expanded Program for Immunization schedule, and 200 JE<br />
vaccination-naive 12- to 24-month-old toddlers were randomized 1:1 to receive JE-CV, containing 4 log10 plaque forming<br />
units, 1 month before or after hepatitis A control vaccine. Neutralizing antibody titers were assessed using PRNT50 (titers<br />
expressed in inverse <strong>of</strong> dilution) before and 28 days after JE-CV, and at months 7 and 12. Results: All 2- to 5-year-olds and<br />
96% <strong>of</strong> 12- to 24-month-olds were seroprotected (titer 10) 28 days after JE-CV administration, and geometric mean titers<br />
(GMT) (95% confidence interval) in these age groups were 2634 (1928-3600) and 281 (219-362), respectively. One year later,<br />
seroprotection rates in the 2 age groups were 97% and 84% and GMTs were 454 and 62.3, respectively. Vaccine-induced<br />
antibodies neutralized a panel <strong>of</strong> wild-type JE isolates. There were no vaccine-related serious adverse events. Reactogenicity<br />
<strong>of</strong> JE-CV was comparable with that <strong>of</strong> the inactivated hepatitis A vaccine. Conclusions: A single administration <strong>of</strong> JE-CV has a<br />
good safety pr<strong>of</strong>ile and elicits a protective immune response in both JE-naive toddlers and JE-primed young children. © <strong>2010</strong><br />
Lippincott Williams & Wilkins.<br />
No.245<br />
Author (s) : Chokkhanchitchai, S., Tangarunsanti, T., Jaovisidha, S., Nantiruj, K., Janwityanujit, S.<br />
Title : The effect <strong>of</strong> religious practice on the prevalence <strong>of</strong> knee osteoarthritis<br />
Source : Clinical Rheumatology, Volume 29, Issue 1, January <strong>2010</strong>, Pages 39-44<br />
Document Type : Article<br />
Keywords : Ethnicity; Radiographic knee osteoarthritis; Religious practice; Symptomatic knee osteoarthritis; WOMAC score<br />
<strong>Abstract</strong> : The aim <strong>of</strong> this study is to evaluate the effect <strong>of</strong> religious practice on the prevalence, severity, and patterns <strong>of</strong> knee<br />
osteoarthritis (OA) in a Thai elderly population with the same ethnicity and culture but different religions. A house-to-house<br />
survey was conducted in two subdistricts <strong>of</strong> Phranakhon Sri Ayutthaya province where inhabitants are a mixture <strong>of</strong> Buddhists<br />
and Muslims. One hundred fifty-three Buddhists and 150 Muslims aged ≥ 50 years were evaluated demographically,<br />
physically, and radiographically. Those suffering knee pains were questioned about severity using the Western Ontario and<br />
McMaster <strong>University</strong> Osteoarthritis Index (WOMAC) scores and examined for their range <strong>of</strong> knee motion. Radiographic knee OA<br />
(ROA) was defined as Kellgren-Lawrence radiographic grade ≥2 while symptomatic knee OA (SOA) was defined as knee<br />
symptoms <strong>of</strong> at least 1 month in a knee with ROA. Muslims had on average a higher number <strong>of</strong> daily religious practices than<br />
their Buddhist neighbors (p
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
counterparts with the same ethnicity but different religious practice. The Muslim way <strong>of</strong> praying since childhood, forcing the<br />
knees into deep flexion, may stretch the s<strong>of</strong>t tissue surrounding the knee and decrease stiffness and contact pressure <strong>of</strong> the<br />
articular cartilage. © 2009 Clinical Rheumatology.<br />
No.246<br />
Author (s) : Cholamjiak, W., Suantai, S.<br />
Title : Convergence theorems from monotone hybrid methods for an infinitely countable family <strong>of</strong> Lipschitz asymptotically quasinonexpansive<br />
mappings<br />
Source : Nonlinear Analysis: Hybrid Systems, Volume 4, Issue 3, August <strong>2010</strong>, Pages 524-530<br />
Document Type : Article<br />
Keywords : Asymptotically quasi-nonexpansive mapping; Common fixed point; Mann iteration process; Monotone hybrid<br />
method; Strong convergence<br />
<strong>Abstract</strong> : In this paper, we prove a weak convergence theorem for the modified Mann iteration process for a uniformly Lipschitzian and<br />
asymptotically quasi-nonexpansive mapping in a uniformly convex Banach space. We also introduce two new kinds <strong>of</strong><br />
monotone hybrid methods and obtain strong convergence theorems for an infinitely countable family <strong>of</strong> uniformly<br />
Lipschitzian and asymptotically quasi-nonexpansive mappings in a Hilbert space. The results <strong>of</strong> this paper improve on and<br />
extend corresponding ones announced by many authors. © 2009.<br />
No.247<br />
Author (s) : Cholamjiak, W., Suantai, S.<br />
Title : Weak and strong convergence theorems for a finite family <strong>of</strong> generalized asymptotically quasi-nonexpansive mappings<br />
Source : Computers and Mathematics with Applications, Volume 60, Issue 7, October <strong>2010</strong>, Pages 1917-1923<br />
Document Type : Article<br />
Keywords : Banach space; Common fixed point; Generalized asymptotically quasi-nonexpansive mapping; Iterative method; Strong<br />
convergence<br />
<strong>Abstract</strong> : In this paper, we introduce a new iterative scheme for finding a common fixed point <strong>of</strong> a finite family <strong>of</strong> generalized<br />
asymptotically quasi-nonexpansive mappings in a uniformly convex Banach space. We establish weak and strong convergence<br />
theorems. Our main results improve and extend the corresponding ones obtained in Schu (1991) [J. Schu, Iterative<br />
construction <strong>of</strong> fixed points <strong>of</strong> asymptotically nonexpansive mapping, J. Math. Anal. Appl. 159 (1991) 407413] and many<br />
others. © <strong>2010</strong> Elsevier Ltd. All rights reserved.<br />
No.248<br />
Author (s) : Chomchai, S., Anusornsuwan, T., Chomchai, C., Plubjui, P.<br />
Title : Relationship <strong>of</strong> Acetaminophen Psi Parameter and Hepatotoxicity Secondary to Acute Acetaminophen Overdose in Thai<br />
Population<br />
Source : CLINICAL TOXICOLOGY, Volume: 48, Issue: 6, Pages: 634-634, Meeting <strong>Abstract</strong>: 146, Published: JUL <strong>2010</strong><br />
Document Type : Meeeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.249<br />
Author (s) : Chomcheon, P., Wiyakrutta, S., Aree, T., Sriubolmas, N., Ngamrojanavanich, N., <strong>Mahidol</strong>, C., Ruchirawat, S., Kittakoop, P.<br />
Title : Curvularides A-E: Antifungal hybrid peptide-polyketides from the endophytic fungus Curvularia geniculate<br />
Source : Chemistry - A European Journal, Volume 16, Issue 36, 24 September <strong>2010</strong>, Pages 11178-11185<br />
Document Type : Article<br />
Keywords : Antifungal agents; Natural products; Peptides; Phytochemistry; Polyketides<br />
<strong>Abstract</strong> : Five new hybrid peptide-polyketides, curvularides A-E (1-5), were isolated from the endophytic fungus Curvularia geniculata,<br />
which was obtained from the limbs <strong>of</strong> Catunaregam tomentosa. Structure elucidation for curvularides A-E (1-5) was<br />
accomplished by analysis <strong>of</strong> spectroscopic data, as well as by single-crystal X-ray crystallography. Curvularide B (2) exhibited<br />
antifungal activity against Candida albicans, and it also showed synergistic activity with a fluconazole drug. © <strong>2010</strong> Wiley-VCH<br />
Verlag GmbH & Co. KGaA. Weinheim.<br />
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No.250<br />
Author (s) : Chomean, S., Potipitak, T., Promptmas, C., Ittarat, W.<br />
Title : Quartz crystal microbalance-based biosensor for the detection <strong>of</strong> α-thalassemia 1 (SEA deletion)<br />
Source : Clinical Chemistry and Laboratory Medicine, Volume 48, Issue 9, 1 September <strong>2010</strong>, Pages 1247-1254<br />
Document Type : Article<br />
Keywords : biosensor; DNA; quartz crystal microbalance; thalassemia<br />
<strong>Abstract</strong> : Background: DNA piezoelectric biosensors have become a promising tool in molecular medicine since they do not require any<br />
label or staining. Here, a DNA piezoelectric biosensor based on a quartz crystal microbalance (QCM) was created to identify<br />
abnormal genes causing α-thalassemia 1 (SEA deletion). Methods: The functionalized gold electrode <strong>of</strong> the quartz crystal was<br />
coated with avidin and the biotinylated DNA probe was attached. The target gene causing α-thalassemia 1 was amplified and<br />
hybridized with the immobilized probe. DNA hybridization was indicated by changes in the quartz resonance frequencies.<br />
Diagnostic ability <strong>of</strong> the new α-thalassemia 1 biosensor was validated using both known and unknown blood samples.<br />
Specificity was tested using samples <strong>of</strong> β-thalassemia and α-thalassemia 2. Stability <strong>of</strong> the sensor was also evaluated.<br />
Results: The new biosensor could clearly identify α-thalassemia 1 (SEA deletion), both carrier and disease states, from the<br />
normal genotype. Identification accuracy was compatible to the standard gel electrophoresis. It was specific only to αthalassemia<br />
1 since no cross reaction was found with β-thalassemia and α-thalassemia 2. The sensor could be kept at room<br />
temperature up to 6 months with consistent identification accuracy. Conclusions: The label free QCM based biosensor was<br />
successfully developed to diagnose an abnormal human globin gene causing α-thalassemia 1 (SEA deletion). Its accuracy,<br />
specificity and sensitivity were comparable to the standard method. Its stable diagnostic potency up to 6 months implied its<br />
field application in thalassemic control program. © <strong>2010</strong> by Walter de Gruyter Berlin New York.<br />
No.251<br />
Author (s) : Chompoothawat, N., Wongthanate, J., Ussawarujikulchai, A., Prapagdee, B.<br />
Title : Removal <strong>of</strong> cadmium ion from aqueous solution by exopolysaccharide-producing bacterium, Ralstonia sp<br />
Source : Fresenius Environmental Bulletin, Volume 19, Issue 12, <strong>2010</strong>, Pages 2919-2923<br />
Document Type : Article<br />
Keywords : Cadmium biosorption; Exopolysaccharides; Metal binding sites; Ralstonia sp<br />
<strong>Abstract</strong> : Bacterial exopolysaccharides (EPS) have an important role in the biosorption <strong>of</strong> heavy metals. The EPS-producing bacterium<br />
Ralstonia sp. TAK1 is a resistant cadmium bacterium isolated from cadmium-contaminated soil at a zinc mine in Thailand.<br />
Ralstonia sp. TAK1 produced the highest amounts <strong>of</strong> EPS at the stationary growth phase at 5.39 g/1 when cultured in a<br />
minimal medium amended with 2% glucose. The stationary cells <strong>of</strong> Ralstonia sp. TAK1 had the highest cadmium removal<br />
efficiency (41.73%) and cadmium adsorption capacity (15.02 mg/g). Cadmium removal efficiency and cadmium adsorption<br />
capacity by EPS were 1.91% and 0.69 mg/g respectively. In addition, the external structure <strong>of</strong> cadmium-adsorbed EPS became<br />
altered when compared to cadmium-unadsorbed EPS. Infrared spectroscopy confirmed the presence <strong>of</strong> functional groups or<br />
metal binding sites on the surface <strong>of</strong> bacterial EPS. Some spectra bands <strong>of</strong> cadmium-adsorbed EPS disappeared in contrast to<br />
cadmium-unadsorbed EPS due to the former being occupied by cadmium ions. These findings suggest that there is potential<br />
for Ralstonia sp. TAK1 cells and their EPS to be used for cadmium biosorption and applied in microbial based remediation <strong>of</strong><br />
cadmium contaminated wastewaters. © by PSP.<br />
No.252<br />
Author (s) : Chongsuvivatwong, V., Yipintsoi, T., Suriyawongpaisal, P., Cheepudomwit, S., Aekplakorn, W., Faramnuayphol, P., Tatsanavivat,<br />
P., Kosulwat, V., Thamthitiwat, S., Nuntawan, C.<br />
Title : Comparison <strong>of</strong> cardiovascular risk factors in five regions <strong>of</strong> Thailand: InterASIA data<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 1, January <strong>2010</strong>, Pages 17-26<br />
Document Type : Article<br />
Keywords : Cardiovascular disease; Ischemic heart disease; Standardized mortality rate; Stroke<br />
<strong>Abstract</strong> : Background: Age- and sex- standardized mortality rate <strong>of</strong> cardiovascular disease (CVD) was high in Bangkok and central<br />
Thailand in the year 2000. This may partially be related to differences in risk factors. Objective: To compare prevalence <strong>of</strong><br />
CVD risk factors among regions in Thailand in the same period. Material and Method: From a survey in 2000 (InterASIA) which<br />
involved 5 regions in Thailand, conventional CVD risk factors were compared multivariate-wise among regions and<br />
subsequently aligned with CVD deaths obtained within similar regions from the registry. Results: Bangkok and a central<br />
province had a higher prevalence <strong>of</strong> the following: hypertension, elevated body mass index, large waist circumference,<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
elevated lipid associated with low density lipoprotein cholesterol and diabetes mellitus. The Northeast had a higher<br />
prevalence <strong>of</strong> smoking, low values <strong>of</strong> high density lipoprotein cholesterol and high triglyceride. Conclusion: Definite regional<br />
differences existed <strong>of</strong> CVD risks and death in Thailand in 2000. Some <strong>of</strong> the metabolic risk factors may be more important<br />
than smoking in the link with CVD death in Thailand.<br />
No.253<br />
Author (s) : Choopong, P., Tesavibul, N., Rodanant, N.<br />
Title : Crystallization after intravitreal ganciclovir injection<br />
Source : Clinical Ophthalmology, Volume 4, Issue 1, <strong>2010</strong>, Pages 709-711<br />
Document Type : Article<br />
Keywords : CMV; Ganciclovir; Intraocular; Intravitreal injection; Toxicity<br />
<strong>Abstract</strong> : Purpose: To report crystal formation as a complication <strong>of</strong> intravitreal ganciclovir injection. Patients and methods: A 73-yearold<br />
female patient with unilateral cytomegalovirus retinitis was treated with intravitreous ganciclovir (4 mg/0.04 mL). Results:<br />
After the intravitreal injection, sudden crystallization was observed in the vitreous humor. The patient experienced marked<br />
reduction in visual acuity and increased intraocular pressure. Despite aqueous paracenthesis and pars plana vitrectomy, optic<br />
atrophy was observed and her visual acuity remained unimproved after 12 months. Conclusion: Crystal formation can occur<br />
as a complication <strong>of</strong> intravitreal ganciclovir injection. Associated retinal and optic nerve damage was found which results in<br />
permanent visual morbidity. © <strong>2010</strong> <strong>2010</strong> Choopong et al, publisher and licensee Dove Medical Press Ltd.<br />
No.254<br />
Author (s) : Chootong, P., Ntumngia, F.B., VanBuskirk, K.M., Xainli, J., Cole-Tobian, J.L., Campbell, C.O., Fraser, T.S., King, C.L., Adams, J.H.<br />
Title : Mapping epitopes <strong>of</strong> the Plasmodium vivax Duffy binding protein with naturally acquired inhibitory antibodies<br />
Source : Infection and Immunity, Volume 78, Issue 3, March <strong>2010</strong>, Pages 1089-1095<br />
Document Type : Article<br />
Keywords : Duffy binding protein; adolescent, adult, aged, allele, article, B lymphocyte, child, controlled study, epitope<br />
mapping, erythrocyte adhesiveness, female, human, male, nonhuman, Plasmodium vivax, priority journal, school child;<br />
Adolescent, Adult, Aged, Amino Acid Sequence, Animals, Antibodies, Neutralizing, Antibodies, Protozoan, Antigens,<br />
Protozoan, Child, Epitope Mapping, Epitopes, B-Lymphocyte, Humans, Middle Aged, Models, Molecular, Molecular Sequence<br />
Data, Papua New Guinea, Plasmodium vivax, Protein Array Analysis, Protein Structure, Tertiary, Protozoan Proteins, Receptors,<br />
Cell Surface, Young Adult<br />
<strong>Abstract</strong> : Plasmodium vivax Duffy binding protein (DBP) is a merozoite microneme ligand vital for blood-stage infection, which makes it<br />
an important candidate vaccine for antibody-mediated immunity against vivax malaria. A differential screen with a linear<br />
peptide array compared the reactivities <strong>of</strong> noninhibitory and inhibitory high-titer human immune sera to identify target<br />
epitopes associated with protective immunity. Naturally acquired anti-DBP-specific serologic responses observed in the<br />
residents <strong>of</strong> a region <strong>of</strong> Papua New Guinea where P. vivax is highly endemic exhibited significant changes in DBP-specific titers<br />
over time. The anti-DBP functional inhibition for each serum ranged from complete inhibition to no inhibition even for hightiter<br />
responders to the DBP, indicating that epitope specificity is important. Inhibitory immune human antibodies identified<br />
specific B-cell linear epitopes on the DBP (SalI) ligand domain that showed significant correlations with inhibitory responses.<br />
Affinity-purified naturally acquired antibodies on these epitopes inhibited the DBP erythrocyte binding function greatly,<br />
confirming the protective value <strong>of</strong> specific epitopes. These results represent an important advance in our understanding <strong>of</strong><br />
part <strong>of</strong> blood-stage immunity to P. vivax and some <strong>of</strong> the specific targets for vaccine-elicited antibody protection. Copyright ©<br />
<strong>2010</strong>, American Society for Microbiology. All Rights Reserved.<br />
No.255<br />
Author (s) : Choowongkomon, K., Theppabutr, S., Songtawee, N., Day, N.P.J., White, N.J., Woodrow, C.J., Imwong, M.<br />
Title : Computational analysis <strong>of</strong> binding between malarial dihydr<strong>of</strong>olate reductases and anti-folates<br />
Source : Malaria Journal, Volume 9, Issue 1, <strong>2010</strong>, Article number 65<br />
Document Type : Article<br />
Keywords : cycloguanil, dihydr<strong>of</strong>olate reductase, Plasmodium falciparum dihydr<strong>of</strong>olate reductase, Plasmodium malariae dihydr<strong>of</strong>olate<br />
reductase, Plasmodium ovale dihydr<strong>of</strong>olate reductase, Plasmodium vivax dihydr<strong>of</strong>olate<br />
reductase, pyrimethamine, trimethoprim, unclassified drug, folic acid antagonist; antibiotic resistance, article, controlled<br />
study, crystal structure, DNA template, enzyme structure, gene mutation, ligand binding, mathematical analysis, molecular<br />
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docking, molecular dynamics, molecular model, Plasmodium vivax, sequence analysis, amino acid<br />
sequence, animal, chemical structure, drug effect, drug resistance, genetics, metabolism, molecular<br />
genetics, mutation, Plasmodium malariae, Plasmodium ovale, structural homology; Amino Acid Sequence, Animals, Drug<br />
Resistance, Folic Acid Antagonists, Models, Molecular, Molecular Sequence Data, Mutation, Plasmodium malariae, Plasmodium<br />
ovale, Pyrimethamine, Sequence Analysis, Structural Homology, Protein, Tetrahydr<strong>of</strong>olate Dehydrogenase<br />
<strong>Abstract</strong> : Background: Plasmodium falciparum readily develops resistance to the anti-folates pyrimethamine and proguanil via a<br />
characteristic set <strong>of</strong> mutations in the dihydr<strong>of</strong>olate reductase (PfDHFR) gene that leads to reduced competitive drug binding<br />
at the enzyme's active site. Analogous mutations can be found in the DHFR gene in isolates <strong>of</strong> Plasmodium vivax (PvDHFR)<br />
although anti-folates have not been widely used for the treatment <strong>of</strong> this infection. Here the interactions between DHFR<br />
inhibitors and modelled structures <strong>of</strong> the DHFR enzymes <strong>of</strong> Plasmodium malariae (PmDHFR) and Plasmodium ovale (PoDHFR)<br />
are described, along with an investigation <strong>of</strong> the effect <strong>of</strong> recently reported mutations within PmDHFR. Methods. DHFR<br />
models for PmDHFR and PoDHFR were constructed using the solved PfDHFR-TS and PvDHFR structures respectively as<br />
templates. The modelled structures were docked with three DHFR inhibitors as ligands and more detailed interactions were<br />
explored via simulation <strong>of</strong> molecular dynamics. Results. Highly accurate models were obtained containing sets <strong>of</strong> residues<br />
that mediate ligand binding which are highly comparable to those mediating binding in known crystal structures. Within this<br />
set, there were differences in the relative contribution <strong>of</strong> individual residues to inhibitor binding. Modelling <strong>of</strong> PmDHFR<br />
mutant sequences revealed that PmDHFR I170M was associated with a significant reduction in binding energy to all DHFR<br />
inhibitors studied, while the other predicted resistance mutations had lesser or no effects on ligand binding. Conclusions.<br />
Binding <strong>of</strong> DHFR inhibitors to the active sites <strong>of</strong> all four Plasmodium enzymes is broadly similar, being determined by an<br />
analogous set <strong>of</strong> seven residues. PmDHFR mutations found in field isolates influenced inhibitor interactions to a varying<br />
extent. In the case <strong>of</strong> the isolated I170M mutation, the loss <strong>of</strong> interaction with pyrimethamine suggests that DHFR-inhibitor<br />
interactions in P. malariae are different to those seen for DHFRs from P. falciparum and P. vivax. © <strong>2010</strong> Choowongkomon et<br />
al; licensee BioMed Central Ltd.<br />
No.256<br />
Author (s) : Chosrowjan, H., Taniguchi, S., Mataga, N., Nakanishi, T., Haruyama, Y., Sato, S., Kitamura, M., Tanaka, F.<br />
Title : Effects <strong>of</strong> the disappearance <strong>of</strong> one charge on ultrafast fluorescence dynamics <strong>of</strong> the FMN binding protein<br />
Source : Journal <strong>of</strong> Physical Chemistry B, Volume 114, Issue 18, 13 May <strong>2010</strong>, Pages 6175-6182<br />
Document Type : Article<br />
Keywords : Average lifetime, Binding proteins, Desulfovibrio vulgaris, Fluorescence dynamics, Fluorescence lifetimes, Geometrical<br />
factors, Hydrogen bondings, Ionic charge, Photo-induced electron transfer, Static structures, Three systems, Ultra-fast, Upconversion<br />
methods, X-ray diffraction method; Amino acids, Binding energy, Electron transitions, Fluorescence, Hydrogen<br />
bonds, Organic acids, X ray diffraction; Proteins; carrier protein, flavine mononucleotide;<br />
article, chemistry, fluorescence, hydrogen bond, X ray crystallography, X ray diffraction; Carrier Proteins, Crystallography, X-<br />
Ray, Flavin Mononucleotide, Fluorescence, Hydrogen Bonding, X-Ray Diffraction<br />
<strong>Abstract</strong> : Crystal structures <strong>of</strong> E13T (Glu13 was replaced by Thr13) and E13Q (Glu13 was replaced by Gln13) FMN binding proteins<br />
(FMN-bp) from Desulfovibrio vulgaris, strain Miyazaki F, were determined by the X-ray diffraction method. Geometrical factors<br />
related to photoinduced electron transfer from Trp32, Tyr35, and Trp106 to the excited isoalloxazine (Iso) were compared<br />
among the three forms <strong>of</strong> FMN-bp. The rate <strong>of</strong> ET is considered to be fastest from Trp32 to Iso* in FMN-bp and then from<br />
Tyr35 and Trp106. The distances between Iso and Trp32 did not change appreciably (0.705-0.712 nm) among WT, E13T, and<br />
E13Q FMN-bps, though the distances between Iso and Tyr35 or Trp106 became a little shorter by ca. 0.01 nm in both<br />
mutated FMN-bps. The distances between the residue at 13 and the ET donors or acceptor in the mutated proteins, however,<br />
changed markedly, compared to WT. Hydrogen bonding pairs and distances between Iso and surrounding amino acids were<br />
not modified when Glu13 was replaced by Thr13 or Gln13. Effects <strong>of</strong> elimination <strong>of</strong> ionic charge at Glu13 on the ultrafast<br />
fluorescence dynamics in E13T and E13Q were investigated comparing to WT, by means <strong>of</strong> a fluorescence up-conversion<br />
method. Fluorescence lifetimes were α1 = 107 fs (α1 = 0.86), α2 = 475 fs (α2 = 0.12), and α3 = 30 ps (α3 = 0.02) in E13T<br />
and α1 = 134 fs (α1 = 0.85), α2 = 746 fs (α2 = 0.12), and α3 = 30 ps (α3 = 0.03) in E13Q, which are compared to the<br />
reported lifetimes in WT, α1 = 168 fs (α1 = 0.95) and α2 = 1.4 ps (α2 = 0.05). Average lifetimes (αAV = α=12or3αiα.,i) were<br />
0.75 ps in E13T, 1.10 ps in E13Q, and 0.23 ps in WT, which implies that αAV was 3.3 times longer in E13T and 4.8 times longer<br />
in E13Q, compared to WT. The ultrafast fluorescence dynamics <strong>of</strong> WT did not change when solvent changed from H2O to<br />
D2O. Static ET rates (inverse <strong>of</strong> average lifetimes) were analyzed with static structures <strong>of</strong> the three systems <strong>of</strong> FMN-bp. Net<br />
electrostatic (ES) energies <strong>of</strong> Iso and Trp32, on which ET rates depend, were 0.0263 eV in WT, 0.322 eV in E13T, and 0.412 eV<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
in E13Q. The calculated ET rates were in excellent agreement with the observed ones in all systems. © <strong>2010</strong> American<br />
Chemical Society.<br />
No.257<br />
Author (s) : Chotjumlong, P., Khongkhunthian, S., Ongchai, S., Reutrakul, V., Krisanaprakornkit, S.<br />
Title : Human β-defensin-3 up-regulates cyclooxygenase-2 expression and prostaglandin E2 synthesis in human gingival fibroblasts<br />
Source : Journal <strong>of</strong> Periodontal Research, Volume 45, Issue 4, August <strong>2010</strong>, Pages 464-470<br />
Document Type : Article<br />
Keywords : Gene regulation; Human β-defensin; Inflammation; Lipid mediator<br />
<strong>Abstract</strong> : Chotjumlong P, Khongkhunthian S, Ongchai S, Reutrakul V, Krisanaprakornkit S. Human β-defensin-3 up-regulates<br />
cyclooxygenase-2 expression and prostaglandin E2synthesis in human gingival fibroblasts. J Periodont Res <strong>2010</strong>; 45: 464-470.<br />
© <strong>2010</strong> John Wiley & Sons A/S Background and Objective: Oral epithelial cells express three antimicrobial peptide<br />
human β-defensins (hBDs) that have previously been demonstrated to exert proinflammatory effects on various immune<br />
cells. We wanted to examine whether hBDs could induce cyclooxygenase-2 (COX-2) expression and prostaglandin E2 (PGE2)<br />
synthesis in non-immune cells, such as human gingival fibroblasts. Material and Methods: Cultured fibroblasts were treated<br />
with different concentrations <strong>of</strong> hBD-1, -2, -3 or interleukin-1β, as a positive control, for various times, in the presence or<br />
absence <strong>of</strong> NS-398, a specific COX-2 inhibitor. The levels <strong>of</strong> COX-1 and COX-2 mRNA expression were analyzed using RT-PCR<br />
and real-time PCR. Whole cell lysates were analyzed for COX-1 and COX-2 protein expression by western blotting. Cell-free<br />
culture supernatants were assayed for PGE2 levels by ELISA. The lactate dehydrogenase assay was performed to determine<br />
the cytotoxicity <strong>of</strong> hBDs. Results: Ten and 40 μg/mL <strong>of</strong> hBD-3 up-regulated COX-2 mRNA and protein expression, consistent<br />
with COX-2 up-regulation by interleukin-1β, whereas hBD-1 and hBD-2 did not. However, COX-1 mRNA and protein were<br />
constitutively expressed. The time-course study revealed that hBD-3 up-regulated COX-2 mRNA and protein expression at 6<br />
and 12 h, respectively. Consistent with COX-2 up-regulation, 10 and 40 μg/mL <strong>of</strong> hBD-3 significantly increased PGE2 levels in<br />
cell-free culture supernatants (p < 0.05), and this was inhibited by NS-398 in a dose-dependent manner. Neither <strong>of</strong> the hBD<br />
concentrations tested in this study was toxic to the cells. Conclusion: These findings indicate that epithelial human βdefensin-3<br />
functions as a proinflammatory mediator in controlling arachidonic acid metabolism in underlying fibroblasts. ©<br />
<strong>2010</strong> John Wiley & Sons A/S.<br />
No.258<br />
Author (s) : Chottidao, M., Limroongreungrat, W.<br />
Title : Comparison <strong>of</strong> Oxygen Uptake between Single and Double Legs Rope Jumping Exercise<br />
Source : MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, Volume: 42, Issue: 5, Supplement: 1, Pages: 547-548, Meeting <strong>Abstract</strong>:<br />
2212, Published: MAY <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.259<br />
Author (s) : Christiansen, K.J., Ip, M., Ker, H.B., Mendoza, M., Hsu, L., Kiratisin, P., Chongthaleong, A., Redjeki, I.S., Quintana, A., Flamm, R.,<br />
Garcia, J., Cassettari, M., Cooper, D., Okolo, P., Morrissey, I.<br />
Title : In vitro activity <strong>of</strong> doripenem and other carbapenems against contemporary Gram-negative pathogens isolated from<br />
hospitalised patients in the Asia-Pacific region: Results <strong>of</strong> the COMPACT Asia-Pacific Study<br />
Source : <strong>International</strong> Journal <strong>of</strong> Antimicrobial Agents, Volume 36, Issue 6, December <strong>2010</strong>, Pages 501-506<br />
Document Type : Article<br />
Keywords : Carbapenem; Doripenem; Enterobacteriaceae; Gram-negative; Pseudomonas; Surveillance<br />
<strong>Abstract</strong> : The Comparative Activity <strong>of</strong> Carbapenems Testing (COMPACT) Study was designed to determine the in vitro potency <strong>of</strong><br />
doripenem compared with imipenem and meropenem against a large number <strong>of</strong> contemporary Gram-negative pathogens<br />
from more than 100 centres across Europe and the Asia-Pacific region and to assess the reliability <strong>of</strong> Etest methodology for<br />
doripenem minimum inhibitory concentration (MIC) determination against these pathogens. Data from eight countries within<br />
the Asia-Pacific region, which collected 1612 bacterial isolates, are presented here. Etest methodology was found to be a<br />
reliable method for MIC determination. Doripenem showed in vitro activity similar to or better than meropenem and at least<br />
four-fold better than imipenem against Enterobacteriaceae. Against Pseudomonas aeruginosa, doripenem was also the most<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
active <strong>of</strong> the three carbapenems in vitro. However, in vitro results do not necessarily correlate with clinical outcome. © <strong>2010</strong><br />
Elsevier B.V. and the <strong>International</strong> Society <strong>of</strong> Chemotherapy.<br />
No.260<br />
Author (s) : Chu, C., Woods, N., Sawasdee, N., Guizouarn, H., Pellissier, B., Borgese, F., Yenchitsomanus, P.-T., Gowrishankar, M., Cordat, E.<br />
Title : Band 3 Edmonton I, a novel mutant <strong>of</strong> the anion exchanger 1 causing spherocytosis and distal renal tubular acidosis<br />
Source : Biochemical Journal, Volume 426, Issue 3, 15 March <strong>2010</strong>, Pages 379-388<br />
Document Type : Article<br />
Keywords : Anion exchanger 1 (AE1); Hereditary disease; Kidney; Membrane protein; Red blood cell; Trafficking<br />
<strong>Abstract</strong> : dRTA (distal renal tubular acidosis) and HS (hereditary spherocytosis) are two diseases that can be caused by mutations in the<br />
gene encoding the AE1 (anion exchanger 1; Band 3). dRTA is characterized by defective urinary acidification, leading to<br />
metabolic acidosis, renal stones and failure to thrive. HS results in anaemia, which may require regular blood transfusions and<br />
splenectomy. Mutations in the gene encoding AE1 rarely cause both HS and dRTA. In the present paper, we describe a novel<br />
AE1 mutation, Band 3 Edmonton I, which causes dominant HS and recessive dRTA. The patient is a compound heterozygote<br />
with the new mutation C479W and the previously described mutation G701D. Red blood cells from the patient presented a<br />
reduced amount <strong>of</strong> AE1. Expression in a kidney cell line showed that kAE1 (kidney AE1) C479W is retained intracellularly. As<br />
kAE1 is a dimer, we performed co-expression studies and found that, in kidney cells, kAE1 C479W and G701D proteins traffic<br />
independently from each other despite their ability to form heterodimers. Therefore the patient carries one kAE1 mutant that<br />
is retained in the Golgi (G701D) and another kAE1 mutant (C479W) located in the endoplasmic reticulum <strong>of</strong> kidney cells, and<br />
is thus probably unable to reabsorb bicarbonate into the blood. We conclude that the C479W mutant is a novel trafficking<br />
mutant <strong>of</strong> AE1, which causes HS due to a decreased cell-surface AE1 protein and results in dRTA due to its intracellular<br />
retention in kidney. © The Authors.<br />
No.261<br />
Author (s) : Chuang, C.-C., Martinez, K., Xie, G., Kennedy, A., Bumrungpert, A., Overman, A., Jia, W., McIntosh, M.K.<br />
Title : Quercetin is equally or more effective than resveratrol in attenuating tumor necrosis factor-α-mediated inflammation and<br />
insulin resistance in primary human adipocytes<br />
Source : American Journal <strong>of</strong> Clinical Nutrition, Volume 92, Issue 6, 1 December <strong>2010</strong>, Pages 1511-1521<br />
Document Type : Article<br />
Keywords : immunoglobulin enhancer binding protein, insulin receptor substrate 1, interleukin 1beta, interleukin 6, interleukin 8, mitogen<br />
activated protein kinase, monocyte chemotactic protein 1, peroxisome proliferator activated receptor gamma, protein<br />
tyrosine phosphatase 1B, quercetin, resveratrol, serine, stress activated protein kinase, tumor necrosis factor alpha;<br />
adipocyte, article, gene expression, glucose transport, human, human cell, inflammation, insulin resistance, obesity, protein<br />
phosphorylation; Adipocytes, Adult, Anti-Inflammatory Agents, Female, Gene<br />
Expression, Glucose, Humans, Inflammation, Insulin, Insulin Resistance, Middle Aged, Phosphorylation, Phytotherapy, Plant<br />
Extracts, PPAR gamma, Quercetin, Signal Transduction, Stilbenes, Transcription, Genetic, Tumor Necrosis Factoralpha,<br />
Vitis, Young Adult; Rice stripe virus, Vitaceae<br />
<strong>Abstract</strong> : Background: Quercetin and trans-resveratrol (trans-RSV) are plant polyphenols reported to reduce inflammation or insulin<br />
resistance associated with obesity. Recently, we showed that grape powder extract, which contains quercetin and trans-RSV,<br />
attenuates markers <strong>of</strong> inflammation in human adipocytes and macrophages and insulin resistance in human adipocytes.<br />
However, we do not know how quercetin and trans-RSV individually affected these outcomes. Objective: The aim <strong>of</strong> this<br />
study was to examine the extent to which quercetin and trans-RSV prevented inflammation or insulin resistance in primary<br />
cultures <strong>of</strong> human adipocytes treated with tumor necrosis factor-α (TNF-α) - an inflammatory cytokine elevated in the<br />
plasma and adipose tissue <strong>of</strong> obese, diabetic individuals. Design: Cultures <strong>of</strong> human adipocytes were pretreated with<br />
quercetin and trans-RSV followed by treatment with TNF-α. Subsequently, gene and protein markers <strong>of</strong> inflammation and<br />
insulin resistance were measured. Results: Quercetin, and to a lesser extent trans-RSV, attenuated the TNF-α-induced<br />
expression <strong>of</strong> inflammatory genes such as interleukin (IL)-6, IL-1β, IL-8, and monocyte chemoattractant protein-1 (MCP-1) and<br />
the secretion <strong>of</strong> IL-6, IL-8, and MCP-1. Quercetin attenuated TNF-α-mediated phosphorylation <strong>of</strong> extracellular signal-related<br />
kinase and c-Jun-NH2 terminal kinase, whereas trans-RSV attenuated only c-Jun-NH2 terminal kinase phosphorylation.<br />
Quercetin and trans-RSV attenuated TNF-α-mediated phosphorylation <strong>of</strong> c-Jun and degradation <strong>of</strong> inhibitory κB protein.<br />
Quercetin, but not trans-RSV, decreased TNF-α-induced nuclear factor-κB transcriptional activity. Quercetin and trans-RSV<br />
attenuated the TNF-α-mediated suppression <strong>of</strong> peroxisome proliferator-activated receptor γ (PPARγ) and PPARγ target<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
genes and <strong>of</strong> PPARγ protein concentrations and transcriptional activity. Quercetin prevented the TNF-α-mediated serine<br />
phosphorylation <strong>of</strong> insulin receptor substrate-1 and protein tyrosine phosphatase-1B gene expression and the suppression <strong>of</strong><br />
insulin-stimulated glucose uptake, whereas trans-RSV prevented only the TNF-α-mediated serine phosphorylation <strong>of</strong> insulin<br />
receptor substrate-1. Conclusion: These data suggest that quercetin is equally or more effective than trans-RSV in attenuating<br />
TNF-α-mediated inflammation and insulin resistance in primary human adipocytes. © <strong>2010</strong> American Society for Nutrition.<br />
No.262<br />
Author (s) : Chuangchaiya, S., Jangpatarapongsa, K., Chootong, P., Sirichaisinthop, J., Sattabongkot, J., Pattanapanyasat, K., Chotivanich, K.,<br />
Troye-Blomberg, M., Cui, L., Udomsangpetch, R.<br />
Title : Immune response to Plasmodium vivax has a potential to reduce malaria severity<br />
Source : Clinical and Experimental Immunology, Volume 160, Issue 2, May <strong>2010</strong>, Pages 233-239<br />
Document Type : Article<br />
Keywords : Antibody response; CD4+ T cells; CD8+ T cells; Gamma delta T cells; Infections<br />
<strong>Abstract</strong> : Summary Plasmodium falciparum infection causes transient immunosuppression during the parasitaemic stage. However, the<br />
immune response during simultaneous infections with both P. vivax and P. falciparum has been investigated rarely. In<br />
particular, it is not clear whether the host's immune response to malaria will be different when infected with a single or<br />
mixed malaria species. Phenotypes <strong>of</strong> T cells from mixed P. vivax-P. falciparum (PV-PF) infection were characterized by flow<br />
cytometry, and anti-malarial antibodies in the plasma were determined by an enzyme-linked immunosorbent assay. We<br />
found the percentage <strong>of</strong> CD3 +δ2+-T cell receptor (TCR) T cells in the acute-mixed PV-PF infection and single P. vivax<br />
infection three times higher than in the single P. falciparum infection. This implied that P. vivax might lead to the host<br />
immune response to the production <strong>of</strong> effector T killer cells. During the parasitaemic stage, the mixed PV-PF infection had the<br />
highest number <strong>of</strong> plasma antibodies against both P. vivax and P. falciparum. Interestingly, plasma from the group <strong>of</strong> single P.<br />
vivax or P. falciparum malaria infections had both anti-P. vivax and anti-P. falciparum antibodies. In addition, antigenic crossreactivity<br />
<strong>of</strong> P. vivax or P. falciparum resulting in antibodies against both malaria species was shown in the supernatant <strong>of</strong><br />
lymphocyte cultures cross-stimulated with either antigen <strong>of</strong> P. vivax or P. falciparum. The role <strong>of</strong> δ2 ± TCR T cells and the<br />
antibodies against both species during acute mixed malaria infection could have an impact on the immunity to malaria<br />
infection. © 2009 British Society for Immunology.<br />
No.263<br />
Author (s) : Chuansumrit, A., Plueksacheeva, T., Hanpinitsak, S., Sangwarn, S., Chatvutinun, S., Suthutvoravut, U., Herabutya, Y., Shearer,<br />
M.J.<br />
Title : Prevalence <strong>of</strong> subclinical vitamin K deficiency in Thai newborns: Relationship to maternal phylloquinone intakes and delivery<br />
risk<br />
Source : Archives <strong>of</strong> Disease in Childhood: Fetal and Neonatal Edition, Volume 95, Issue 2, March <strong>2010</strong>, Pages F104-F108<br />
Document Type : Article<br />
Keywords : decarboxyprothrombin, phytomenadione; adult, article, controlled study, delivery, dietary intake, disease<br />
predisposition, female, food frequency questionnaire, high risk infant, human, immunoassay, infant, major clinical<br />
study, male, newborn, nutritional assessment, nutritional status, prevalence, priority journal, risk factor, Thailand, umbilical<br />
cord blood, vitamin K deficiency; Adult, Antifibrinolytic Agents, Biological Markers, Birth Injuries, Diet, Enzyme-Linked<br />
Immunosorbent Assay, Female, Fetal Growth Retardation, Humans, Infant, Newborn, Infant, Premature, Maternal Nutritional<br />
Physiological Phenomena, Pregnancy, Prevalence, Protein Precursors, Prothrombin, Risk Factors, Thailand, Vitamin K 1, Vitamin<br />
K Deficiency Bleeding, Vitamins, Young Adult<br />
<strong>Abstract</strong> : Background: Vitamin K deficiency bleeding (VKDB) in infants is a rare but serious worldwide problem, particularly in Southeast<br />
Asia. Apart from exclusive breast feeding, little is known <strong>of</strong> the matern<strong>of</strong>etal risk factors that predispose infants to VKDB.<br />
Objectives: To assess (a) the relationships between functional vitamin K insufficiency in a large cohort <strong>of</strong> Thai mothers to that<br />
<strong>of</strong> their newborn infants and (b) the importance <strong>of</strong> delivery risk factors and maternal intakes <strong>of</strong> vitamin K as determinants <strong>of</strong><br />
neonatal vitamin K status. Methods: Vitamin K status was assessed by measuring undercarboxylated prothrombin (protein<br />
induced by vitamin K absence/antagonist-II (PIVKA-II)) in 683 mothers and in the cord blood <strong>of</strong> their babies by sensitive<br />
immunoassay. Dietary phylloquinone (vitamin K1; K1) intakes were assessed in 106 <strong>of</strong> these mothers by food frequency<br />
questionnaire. Results: Babies were categorised as 'normal' (n=590) or 'high risk' (n=93) according to birth weight and delivery<br />
type. PIVKA-II was detectable (>0.15 arbitrary units (AU)/ml) in 85 mothers (12.4%) and 109 babies (16.0%) with median<br />
levels <strong>of</strong> 0.78 and 1.04 AU/ml in mothers and babies, respectively. 'High-risk' babies had a higher median detectable PIVKA-II<br />
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concentration than 'normal-risk' babies (3.1 vs 1.0 AU/ml, p=0.02) and a higher prevalence <strong>of</strong> clinically relevant (>5.0<br />
AU/ml) concentrations (p=0.006). Mothers with K1 intakes below the US recommended 'adequate intake' for pregnancy<br />
(<90 μg/day) had a higher prevalence <strong>of</strong> detectable PIVKA-II (18.8%) than those with adequate intakes (3.3%) (p=0.01).<br />
Conclusions: Functional, clinically relevant, vitamin K insufficiency was more common in 'high-risk' than 'normal-risk'<br />
newborns. Vitamin K insufficiency in mothers was linked to lower dietary K1 intakes during pregnancy.<br />
No.264<br />
Author (s) : Chuansumrit, A., Puripokai, C., Butthep, P., Wongtiraporn, W., Sasanakul, W., Tangnararatchakit, K., Chunhakan, S., Yoksan, S.<br />
Title : Laboratory predictors <strong>of</strong> dengue shock syndrome during the febrile stage<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 2, March <strong>2010</strong>, Pages 326-332<br />
Document Type : Article<br />
Keywords : Dengue hemorrhagic fever; Dengue shock syndrome; Predictors<br />
<strong>Abstract</strong> : The clinical manifestations <strong>of</strong> dengue hemorrhagic fever (DHF) consist <strong>of</strong> three successive stages: febrile, toxic and<br />
convalescent. The toxic stage is the critical period, which may manifest as circulatory disturbance or even pr<strong>of</strong>ound shock in<br />
some patients. We attempted to determine predictors for the risk <strong>of</strong> dengue shock syndrome (DSS) during the febrile stage.<br />
One hundred one children with acute febrile illness were enrolled in the study, with a mean age <strong>of</strong> 11 years old. The<br />
diagnosis included dengue fever (DF) 21 cases, DHF grade I 30 cases, DHF grade II 33 cases, DHF grades III and IV 10 cases;<br />
children with other febrile illnesses (OFI) 7 cases were used as controls. Complete blood counts, coagulation tests, von<br />
Willebrand factor antigens (VWF:Ag) and ristocetin c<strong>of</strong>actor activity (VWF:Rc<strong>of</strong>) were determined daily during hospitalization<br />
and 2-4 weeks after discharge from the hospital. The results revealed any one <strong>of</strong> the following abnormal laboratory findings<br />
during the febrile stage served as a predictor for risk <strong>of</strong> DSS: increase in hematocrit >25%, a platelet count 44 seconds, a prothrombin time >14 seconds, a thrombin time >16 seconds or a<br />
VWF:Ag or VWF:Rc<strong>of</strong> >210%. The relative risk ranged from 4.8 to 10.9. Simple laboratory investigations with complete blood<br />
count, coagulation test or the more sophisticated von Willebrand factor, are helpful in predicting the risk for DSS during the<br />
febrile stage.<br />
No.265<br />
Author (s) : Chuansumrit, A., Teeraratkul, S., Wanichkul, S., Treepongkaruna, S., Sirachainan, N., Pakakasama, S., Nuntnarumit, P., Hongeng,<br />
S.<br />
Title : Recombinant-activated factor VII for control and prevention <strong>of</strong> hemorrhage in nonhemophilic pediatric patients<br />
Source : Blood Coagulation and Fibrinolysis, Volume 21, Issue 4, June <strong>2010</strong>, Pages 354-362<br />
Document Type : Article<br />
Keywords : Children; Hemorrhage; Infants; Invasive procedure; Newborns; Recombinant factor-activated VIIa<br />
<strong>Abstract</strong> : A total <strong>of</strong> 108 episodes among 103 nonhemophilic pediatric patients (nine newborns, 16 infants and 78 children) treated with<br />
recombinant factor-activated VII (rFVIIa) were evaluated retrospectively. These episodes were divided into two groups: group 1<br />
included 86 occurrences for hemorrhagic control <strong>of</strong> ongoing massive bleeding due to thrombocytopenia and coagulopathy<br />
unresponsive to blood component therapy in patients with dengue hemorrhagic fever, life-threatening, intraoperative and<br />
postoperative bleeding; group 2 included 22 episodes for prevention <strong>of</strong> hemorrhage with invasive procedures in patients with<br />
chronic liver disease and associated coagulopathy, and patients without preexisting hemostatic disorder but at high risk due<br />
to their underlying diagnosis and required surgical intervention. The effective control <strong>of</strong> hemostasis response rate in group 1<br />
was significantly lower than in group 2. The median total dose per kilogram <strong>of</strong> rFVIIa group 1 was twice that <strong>of</strong> group 2. The<br />
overall case-fatality rate related to bleeding or underlying conditions was 31.1% (32/103). Adverse events were observed in<br />
three patients (2.9%) receiving rFVIIa for control <strong>of</strong> intraoperative and postoperative bleeding in the setting <strong>of</strong> corrective<br />
cardiac surgery. These results support the safety and potential benefit <strong>of</strong> rFVIIa for control and prevention <strong>of</strong> hemorrhage in<br />
pediatric patients without congenital hemophilia. © <strong>2010</strong> Wolters Kluwer Health | Lippincott Williams & Wilkins.<br />
No.266<br />
Author (s) : Chuansumrit, A.; Tangnararachakit, K.; Sirachainan, N.; Khositseth, A.; Kuptanon, T.; Wanitkun, S.; Withurawanit, W.; Songdej, D.<br />
Title : DENGUE HEMORRHAGIC FEVER IN HEMOPHILIC PATIENTS: AGGRAVATION OF BLEEDING RISK<br />
Source : HAEMATOLOGICA-THE HEMATOLOGY JOURNAL, Volume: 95, Supplement: 2, Pages: 72-72, Meeting <strong>Abstract</strong>: 0179, Published:<br />
JUN <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
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<strong>Abstract</strong> : –<br />
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No.267<br />
Author (s) : Chuaychoo, B., Wongsurakiat, P., Nana, A., Kositanont, U., Maranetra, K.N.<br />
Title : The immunogenicity <strong>of</strong> intradermal influenza vaccination in COPD patients<br />
Source : Vaccine, Volume 28, Issue 24, 28 May <strong>2010</strong>, Pages 4045-4051<br />
Document Type : Article<br />
Keywords : COPD; Influenza vaccine; Intradermal<br />
<strong>Abstract</strong> : We evaluated the immunogenicity <strong>of</strong> a reduced-dose intradermal trivalent, inactivated, split-virion seasonal influenza vaccine<br />
compared to that <strong>of</strong> a conventional intramuscular vaccination in chronic obstructive pulmonary disease (COPD) patients. One<br />
hundred and fifty-six COPD patients randomly received either 0.2 ml (6 μg hemagglutinin (HA) per strain) split into two-site<br />
intradermal (ID) injections or a single 0.5 ml (15 μg HA per strain) intramuscular (IM) injection. Geometric mean titers,<br />
seroconversion factors, seroconversion rates and seroprotection rates at 4 weeks post-vaccination in the ID group were less<br />
than those in the IM group. Only the seroconversion factor to influenza B in the ID group was statistically less than in the IM<br />
group (18.8 in the ID group, n = 81 versus 37.3 in the IM group, n = 75, p = 0.045). Nevertheless, each strain <strong>of</strong> the ID<br />
vaccination met all the Committee for Proprietary Medicinal Products (CPMP) criteria. Seroprotection rates were above 60%<br />
throughout the year in influenza A (H3N2), for at least 6 months in influenza A (H1N1) and at least 4 weeks in influenza B in<br />
both ID and IM groups. The reduced-dose intradermal vaccination may be considered for use in COPD patients in a vaccine<br />
shortage situation. © <strong>2010</strong> Elsevier Ltd. All rights reserved.<br />
No.268<br />
Author (s) : Chuayjan, W., Pothiphan, S., Wiwatanapataphee, B., Wu, Y.H.<br />
Title : Numerical simulation <strong>of</strong> granular flow during filling and discharging <strong>of</strong> a silo<br />
Source : <strong>International</strong> Journal <strong>of</strong> Pure and Applied Mathematics, Volume 62, Issue 3, <strong>2010</strong>, Pages 347-364<br />
Document Type : Article<br />
Keywords : Discrete element method; Granular flow; Mathematical modelling; Right-conical silo<br />
<strong>Abstract</strong> : In this study, we simulate the static and dynamic processes <strong>of</strong> granular flow during filling and discharging <strong>of</strong> a vertical-sided<br />
silo with conical hopper bottom. The granular material is an assembly <strong>of</strong> 7,500 soybeans. Based on the discrete element<br />
method, the governing equations for the granular flow are solved by the centred finite difference scheme. The effects <strong>of</strong> inlet<br />
flow rate and bottom angles on pressure distribution on the hopper wall throughout the static process <strong>of</strong> material filling into<br />
the silo are investigated. Influences <strong>of</strong> bottom angle on the discharging problem in a varied mass-flow silo and a constant<br />
mass-flow silo are also discussed. © <strong>2010</strong> Academic <strong>Publications</strong>.<br />
No.269<br />
Author (s) : Chudtong, M., Lenbury, Y., Ratanakul, C.<br />
Title : Stability and periodicity in a model <strong>of</strong> bone remodeling under impulsive PTH control<br />
Source : <strong>International</strong> Conference on Applied Computer Science – Proceedings, <strong>2010</strong>, Pages 152-157<br />
Document Type : Conference Paper<br />
Keywords : Asymptotic stability; Bone remodeling; Impulsive differential equation models; Permanence<br />
<strong>Abstract</strong> : In this paper, a mathematical model <strong>of</strong> bone remodeling process, which incorporates the effect <strong>of</strong> impulsive application <strong>of</strong><br />
parathyroid hormone supplementary treatments, is analyzed in terms <strong>of</strong> the boundedness, asymptotic stability, permanence,<br />
and oscillatory behavior. Conditions under which different dynamic behavior may be expected are shown to be sensitive to<br />
the period and amplitude <strong>of</strong> the hormone supplements so that the variation <strong>of</strong> these parameters are crucial <strong>of</strong> the proper<br />
management and control <strong>of</strong> this complex system which is an essential component <strong>of</strong> the human life.<br />
No.270<br />
Author (s) : Chuen-Im, T., Areekijseree, M., Chongthammakun, S., Graham, S.V.<br />
Title : Aerobic bacterial infections in captive juvenile green turtles (Chelonia mydas) and hawksbill turtles (Eretmochelys imbricata)<br />
from Thailand<br />
Source : Chelonian Conservation and Biology, Volume 9, Issue 1, June <strong>2010</strong>, Pages 135-142<br />
Document Type : Article<br />
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Keywords : Bacteria (microorganisms), Chelonia mydas, Cheloniidae, Eretmochelys imbricata, Negibacteria, Posibacteria, Testudines<br />
<strong>Abstract</strong> : Investigation <strong>of</strong> the total <strong>of</strong> 53 juvenile sea turtles (30 green turtles and 2 hawksbill turtles) found signs <strong>of</strong> several clinical<br />
diseases. Ultrastructure and histological observation <strong>of</strong> these tissues revealed that they were infected with one type <strong>of</strong> yeast<br />
and numerous bacteria. Using aerobic microbiological culture techniques, 10 types <strong>of</strong> Gram-positive and Gram-negative<br />
bacteria were identified, which may be responsible for disease and subsequent death <strong>of</strong> juvenile sea turtles at the<br />
conservation center. © <strong>2010</strong> Chelonian Research Foundation.<br />
No.271<br />
Author (s) : Chularojanamontri, L., Tuchinda, P., Kulthanan, K., Manuskiatti, W.<br />
Title : Generalized molluscum contagiosum in an HIV patient treated with diphencyprone<br />
Source : Journal <strong>of</strong> Dermatological Case Reports, Volume 4, Issue 4, <strong>2010</strong>, Pages 60-62<br />
Document Type : Article<br />
Keywords : AIDS; Diphencyprone; DPC; HIV; Molluscum contagiosum; Poxvirus; Treatment; Virus<br />
<strong>Abstract</strong> : Background: Diphencyprone is a universal contact immunotherapy. The mechanism <strong>of</strong> action is based on an induction <strong>of</strong> the<br />
delayed-type hypersensitivity. Diphencyprone has been used in various forms for treatments <strong>of</strong> recalcitrant and facial warts,<br />
and alopecia areata. However, this treatment modality has not been generally used in immunocompromised patients. Main<br />
observation: The present report demonstrated the efficacy <strong>of</strong> diphencyprone immunotherapy on the treatment <strong>of</strong><br />
generalized molluscum contagiosum in a human immunodeficiency virus (HIV)-infected patient. Minimal and transient side<br />
effects including pruritus, postinflammatory hyperpigmentation and irritation were noted. Conclusion: Diphencyprone contact<br />
immunotherapy appears to be a possible alternative treatment <strong>of</strong> widespread molluscum contagiosum in<br />
immunocompromised patients.<br />
No.272<br />
Author (s) : Chularojanamontri, L., Tuchinda, P., Triwongwaranat, D., Pinkaew, S., Kulthanan, K.<br />
Title : Diagnostic significance <strong>of</strong> colloid body deposition in direct immun<strong>of</strong>luorescence<br />
Source : Indian Journal <strong>of</strong> Dermatology, Venereology and Leprology, Volume 76, Issue 4, July <strong>2010</strong>, Pages 373-377<br />
Document Type : Article<br />
Keywords : Colloid bodies; direct immun<strong>of</strong>luorescence; interface dermatitis<br />
<strong>Abstract</strong> : Background: Colloid bodies (CB) in direct immun<strong>of</strong>luorescence (DIF) studies are usually found in interface dermatitis.<br />
Furthermore, CB can be found in various skin diseases and even in normal skin. Aim: To evaluate the diagnostic value <strong>of</strong> CB<br />
deposits in DIF studies. Methods: From 1996-2007, data from 502 patients where DIF studies showed immunoreactants at CB<br />
were enrolled. The definite diagnoses <strong>of</strong> these patients were based on clinical, histopathological and immun<strong>of</strong>luorescent<br />
findings. The results <strong>of</strong> DIF studies were analyzed. Results: Immunoreactants at CB were detected in 44.4%, 43.8%, 4.2%,<br />
3.8%, and 2.2% <strong>of</strong> interface dermatitis, vasculitis, autoimmune vesiculobullous disease, panniculitis, and<br />
scleroderma/morphea, respectively. The most common immunoreactant deposit <strong>of</strong> all diseases was Immunoglobulin M (IgM).<br />
Brighter intensity and higher quantity <strong>of</strong> CB was detected frequently in the group with interface dermatitis. Conclusions:<br />
Immunoreactant deposits at CB alone can be found in various diseases but a strong intensity and high quantity favor the<br />
diagnosis <strong>of</strong> interface dermatitis. CB plus dermoepidermal junction (DEJ) deposits are more common in interface dermatitis<br />
than any other disease. Between lichen planus (LP) and discoid lupus erythematosus (DLE), CB alone is more common in LP;<br />
whereas, CB plus DEJ and superficial blood vessel (SBV) is more common in DLE. The most common pattern in both diseases<br />
is CB plus DEJ. The quantity and intensity <strong>of</strong> CB in LP is higher than in DLE.<br />
No.273<br />
Author (s) : Chularojanamontri, L., Kulthanan, K., Tuchinda, P., Manuskiatti, W.<br />
Title : Treatment <strong>of</strong> generalized molluscum contagiosum in all HIV patient with diphencyprone<br />
Source : JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY, Volume: 62, Issue: 3, Supplement: S, Pages: AB88-AB88, Published:<br />
MAR <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.274<br />
Author (s) : Chulavatnatol, S., Malathum, K., Kiertiburanakul, S., Sripha, K., Lawanprasert, P.<br />
Title : Bioequivalence <strong>of</strong> indinavir capsules in healthy volunteers<br />
Source : Asian Biomedicine, Volume 4, Issue 1, February <strong>2010</strong>, Pages 98-101<br />
Document Type : Article<br />
Keywords : Bioequivalence; Indinavir; Pharmacokinetics; Protease inhibitors; Thai volunteers<br />
<strong>Abstract</strong> : Background: Indinavir, one component in the HAART regimen, plays an important role in the current treatment <strong>of</strong> HIVinfection<br />
and AIDS. Availability and accessibility <strong>of</strong> qualified generic indinavir to patients may be the keys for the success <strong>of</strong><br />
treatment. Objective: Compare the rate and extent <strong>of</strong> absorption <strong>of</strong> a generic indinavir formulation with those <strong>of</strong> an original<br />
formulation in healthy Thai volunteers. Method: A randomized, two-period, two-treatment, two-sequence, crossover study<br />
with a two-week washout period was performed. A single dose <strong>of</strong> 2x400 mg indinavir capsules <strong>of</strong> each formulation was<br />
administered to 24 volunteers after an overnight fast. Indinavir plasma concentrations up to 10 hours postdose were<br />
determined using high-performance liquid chromatography. Relevant pharmacokinetic parameters were derived and tested for<br />
statistically significant differences using ANOVA and criteria <strong>of</strong> bioequivalence determination were applied. Results: No<br />
statistically significant differences were demonstrated for pharmacokinetic parameters including Cmax, Tmax, AUC0-t, and<br />
AUC0-8 derived from the two formulations (n=23, p>0.05). The criteria <strong>of</strong> bioequivalence determination i.e., the 90%<br />
confidence intervals on the mean ratio (generic/original formulation) <strong>of</strong> natural logarithmtransformed values <strong>of</strong> Cmax, AUC0-t<br />
and AUC0-∞ were 86.3-106.5%, 94.0-108.5%, and 93.9-108.5%, respectively. Conclusion: As the mean ratios <strong>of</strong> Cmax, AUC0-t<br />
and AUC0-∞ <strong>of</strong> the generic and original formulations were entirely within the guideline range <strong>of</strong> bioequivalence (80.0-125.0%),<br />
the two formulations were considered bioequivalent in terms <strong>of</strong> rate and extent <strong>of</strong> absorption.<br />
No.275<br />
Author (s) : Chumpathong, S., Sukavanicharat, P., Butmangkun, W., Suraseranivongse, S., Raksakietisak, M., Rushatamukayanunt, P.,<br />
Sirivanasandha, B.<br />
Title : Effectiveness <strong>of</strong> endotracheal-tube size by age-based formula for Thai pediatric cardiac patients: a retrospective study<br />
Source : ASIAN BIOMEDICINE, Volume: 4, Issue: 5, Pages: 765-771, Published: OCT <strong>2010</strong><br />
Document Type : Article<br />
Keywords : Age-based formula; anesthesia; cardiac patients; endotracheal tube; pediatric<br />
<strong>Abstract</strong> : Background: Pediatric patients with congenital heart diseases may have pathological airway abnormality and delayed<br />
development. To predict the appropriate size <strong>of</strong> endotracheal tube (ETT), a formula between diameter and age has been<br />
widely used for Western normal children. However, it is unclear whether this age-based (AB) formula is applicable to Thai<br />
pediatric cardiac patients. Objective: Evaluate the effectiveness <strong>of</strong> uncuffed ETT size by AB formula for pediatric cardiac<br />
patients. Methods: A retrospective study was conducted using 320 cases <strong>of</strong> non-cardiac and cardiac patients aged 2-7 years<br />
old who were orally intubated with a regular uncuffed ETT at Siriraj Hospital, Thailand. The exclusion criteria were history <strong>of</strong><br />
tracheostomy, upper airway obstruction, and expected difficult intubation. Demographic data and final ETT used were<br />
recorded. Results: The tube-size predicted by the AB formula could be applied to 54.4% <strong>of</strong> non-cardiac and 48.1% <strong>of</strong> cardiac<br />
patients (p= 0.314), whereas three sizes <strong>of</strong> tubes (one above and one below the predicted size) covered 96.9% and 94.4% <strong>of</strong><br />
non-cardiac and cardiac patients, respectively (p = 0.413). The ETT with 0.5 mm in ID larger than the predicted size were more<br />
<strong>of</strong>ten used in 35.0% <strong>of</strong> cardiac patients compared with 22.5% <strong>of</strong> non-cardiac patients (p= 0.019). There were no significant<br />
differences between methods using age (actual, round-up, and truncated) to calculate the AB formula. The Pearson's<br />
correlation between the ID <strong>of</strong> the ETT with height in non-cardiac and cardiac patients were 0.430 and 0.683, respectively (p<br />
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : Fatty acid binding proteins are considered to be promising vaccine candidates against trematodiasis. In order to provide<br />
additional information about their function in Fasciola gigantica we performed a comparative analysis <strong>of</strong> FgFABP1 and<br />
FgFABP3, two is<strong>of</strong>orms with quite different isoelectric points <strong>of</strong> 49 and 99 and 67% sequence identity. Both are expressed in<br />
the juvenile and adult parasite but differ in their tissue-specific distribution. In addition, the sequence <strong>of</strong> FABP3 is identical in<br />
F. hepatica and F. gigantica indicating the protein's functional importance in this genus. Immune sera produced against soluble<br />
recombinant FgFABPs reacted with 14 kDa antigens in crude worm, soluble egg, cirrus sac extracts, and excretion/secretion<br />
product. Both FgFABPs were located in the parenchyma <strong>of</strong> the parasite but in addition, FgFABP1 was abundant in testes and<br />
spermatozoa while FgFABP3 was abundant in vitelline cells, eggs, and caecal epithelium. Mass spectrometry identified<br />
FgFABP1 and FgFABP3 in the ES product whereas only FgFABP3 was identified in egg extract. Serum samples <strong>of</strong> an<br />
experimentally infected rabbit reacted from week 6 post-infection with FgFABP3 and from week 12 with FgFABP1 while sera<br />
<strong>of</strong> infected sheep were not reactive. The results suggest differences in the biological functions <strong>of</strong> these 2 is<strong>of</strong>orms and<br />
differences in the host/parasite interaction that should be considered for their potential as vaccines against fascioliasis. ©<br />
Cambridge <strong>University</strong> Press <strong>2010</strong>.<br />
No.277<br />
Author (s) : Churdchomjan, W., Kheolamai, P., Manochantr, S., Tapanadechopone, P., Tantrawatpan, C., U-pratya, Y., Issaragrisil, S.<br />
Title : Comparison <strong>of</strong> endothelial progenitor cell function in type 2 diabetes with good and poor glycemic control<br />
Source : BMC Endocrine Disorders, Volume 10, 7 April <strong>2010</strong>, Article number 5<br />
Document Type : Article<br />
Keywords : 1,1' dioctadecyl 3,3,3',3' tetramethylindocarbocyanine, acetyl low density lipoprotein, CD34 antigen, glucose, hemoglobin<br />
A1c, Ulex europaeus agglutinin, Ulex europaeus agglutinin 1, unclassified drug, vasculotropin receptor 2;<br />
adult, aged, apoptosis, article, cell count, cell function, cell proliferation, cell structure, cell viability, clinical<br />
article, concentration response, controlled study, endothelial dysfunction, endothelium cell, female, flow cytometry, glucose<br />
blood level, glycemic control, human, human cell, hyperglycemia, in vitro study, male, non insulin dependent diabetes<br />
mellitus, protein expression, quantitative analysis, stem cell<br />
<strong>Abstract</strong> : Background: Endothelial progenitor cells (EPCs) play an important role in vascular repair and a decrease in the number <strong>of</strong><br />
EPCs is observed in type 2 diabetes. However, there is no report on the change <strong>of</strong> EPCs after glycemic control. This study<br />
therefore aimed to investigate the EPC number and function in patients with good and poor glycemic control.Methods: The<br />
number <strong>of</strong> EPCs was studied using flow cytometry by co-expression <strong>of</strong> CD34 and VEGFR2. The EPCs were cultured and<br />
characterized by the expression <strong>of</strong> UEA-I, CD34, VEGFR2, vWF and Dil-Ac-LDL engulfment, as well as the ability to form<br />
capillary-like structures. An in vitro study on the effect <strong>of</strong> hyperglycemia on the proliferation and viability <strong>of</strong> the cultured EPCs<br />
was also performed.Results: The number <strong>of</strong> EPCs in type 2 diabetes was significantly decreased compared with healthy<br />
controls and there was an inverse correlation between the EPC numbers and plasma glucose, as well as HbA1C. The number<br />
and function <strong>of</strong> EPCs in patients with good glycemic control were recovered compared with those with poor glycemic control.<br />
When glucose was supplemented in the culture in vitro, there was a negative effect on the proliferation and viability <strong>of</strong> EPCs,<br />
in a dose-dependent manner, whereas the enhancement <strong>of</strong> apoptosis was observed.Conclusion: There was EPC dysfunction<br />
in type 2 diabetes which might be improved by strict glycemic control. However, the circulating EPC number and proliferative<br />
function in patients with good glycemic control did not reach the level in healthy controls. © <strong>2010</strong> Churdchomjan et al;<br />
licensee BioMed Central Ltd.<br />
No.278<br />
Author (s) : Chusattayanond, A.D.A., Boonsilp, S., Kasisit, J., Boonmee, A., Warit, S.<br />
Title : Thai Acanthamoeba isolate (T4) induced apoptotic death in neuroblastoma cells via the Bax-mediated pathway<br />
Source : Parasitology <strong>International</strong>, Volume 59, Issue 4, December <strong>2010</strong>, Pages 512-516<br />
Document Type : Article<br />
Keywords : Acanthamoeba; Apoptosis; Bax; Bcl-2; Caspase; T4 genotype<br />
<strong>Abstract</strong> : A Thai Acanthamoeba isolate named AS recovered from a corneal scraping <strong>of</strong> a keratitis patient was genotypically determined<br />
as T4. AS trophozoites were used for studying Acanthamoeba-induced apoptosis in mouse neuroblastoma NA cells during in<br />
vitro co-cultivation. The Acanthamoeba-exposed NA cells showed signs <strong>of</strong> apoptosis including cell shrinkage, nuclear<br />
condensation and DNA fragmentation. The effect was confirmed by DNA laddering electrophoresis. Involvement <strong>of</strong> caspase<br />
enzymes and mitochondrial pro- and anti-apoptotic proteins (Bax and Bcl-2) in AS-induced apoptosis was determined. The<br />
use <strong>of</strong> Z-VAD-FMK, a pan-caspase inhibitor, significantly reduced the apoptotic effect, while Bax/Bcl-2 ratio analysis showed a<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
significant increase in the expression <strong>of</strong> apoptotic proteins in AS-exposed NA cells. These results strongly indicated that<br />
apoptosis induced by AS trophozoites is caspase-dependent and is mediated by over-expression <strong>of</strong> pro-apoptotic proteins in<br />
the mitochondrial pathway. This is the first report on the role <strong>of</strong> Bax in mediating apoptosis induced by Acanthamoeba. ©<br />
<strong>2010</strong> Elsevier Ireland Ltd.<br />
No.279<br />
Author (s) : Chuthapisith, S., Eremin, J., El-Sheemey, M., Eremin, O.<br />
Title : Breast cancer chemoresistance: Emerging importance <strong>of</strong> cancer stem cells<br />
Source : Surgical Oncology, Volume 19, Issue 1, March <strong>2010</strong>, Pages 27-32<br />
Document Type : Review<br />
Keywords : Breast cancer; Cancer stem cells; Chemoresistance<br />
<strong>Abstract</strong> : Cancer stem cells (CSCs) have recently been documented in solid tumours. Evidence has suggested that CSCs are involved in<br />
carcinogenesis, tumour invasion and metastases, and resistance to various forms <strong>of</strong> therapies, including chemotherapy. Breast<br />
CSCs are characterised by the expression <strong>of</strong> CD44 but lack <strong>of</strong> CD24 (CD44+/CD24- cells). The mechanisms involved in<br />
chemoresistance <strong>of</strong> breast CSCs are complex and not clearly defined. Overexpression <strong>of</strong> ABC transporters, detoxification<br />
enzymes (aldehyde dehydrogenase), low cell turn over rate and the ability to activate the DNA check point response are<br />
possibly all involved. Innovative therapies, based on a better understanding <strong>of</strong> CSCs, should lead to enhanced and long-term<br />
cure rates in breast cancer. © 2009 Elsevier Ltd.<br />
No.280<br />
Author (s) : Chutipongtanate, S., Thongboonkerd, V.<br />
Title : Establishment <strong>of</strong> a novel colorimetric assay for high-throughput analysis <strong>of</strong> calcium oxalate crystal growth modulation<br />
Source : Analyst, Volume 135, Issue 6, June <strong>2010</strong>, Pages 1309-1314<br />
Document Type : Article<br />
Keywords : calcium oxalate, citrate sodium, citric acid; article, chemistry, colorimetry, crystallization, high throughput<br />
screening, instrumentation, methodology, particle size; Calcium Oxalate, Citrates, Colorimetry, Crystallization, High-Throughput<br />
Screening Assays, Particle Size<br />
<strong>Abstract</strong> : Crystal growth is one <strong>of</strong> the most important mechanisms <strong>of</strong> calcium oxalate (CaOx) kidney stone formation. However,<br />
identification and characterization <strong>of</strong> CaOx crystal growth modulators were under-investigated in the past, probably due to<br />
the lack <strong>of</strong> an assay that could measure modulatory activities <strong>of</strong> a large number <strong>of</strong> candidates simultaneously. We therefore<br />
developed such a high-throughput assay based on colorimetric measurements <strong>of</strong> free calcium ions using Arsenazo III in a 96well<br />
microplate, and a microplate reader (λ620 nm). CaOx monohydrate (COM) was crystallized in the absence (blank<br />
control) or presence <strong>of</strong> 5 mM sodium citrate (Na3Citrate) (positive control) or 5 mM sodium chloride (NaCl) (negative control).<br />
The results showed that Na 3Citrate, but not NaCl, could significantly inhibit COM crystal growth. The reliability <strong>of</strong> this novel<br />
assay was validated by quantitative analysis <strong>of</strong> COM crystal size. Degree <strong>of</strong> the inhibitory activity <strong>of</strong> Na 3Citrate obtained from<br />
our novel assay was comparable to that obtained from the reference method (34.5 ± 4.3 vs. 40.0 ± 1.6%Inhibition,<br />
respectively; p was not significant). The high-throughput capability <strong>of</strong> the novel assay was demonstrated by simultaneous<br />
measurement <strong>of</strong> 33 samples (a triplicated set <strong>of</strong> 11 different concentrations <strong>of</strong> Na 3Citrate) with linear correlation between<br />
Na3Citrate concentrations and COM crystal growth inhibitory activities (r = 0.893; p < 0.0001). In summary, we have<br />
successfully established a novel assay for high-throughput analysis <strong>of</strong> CaOx crystal growth modulatory activities <strong>of</strong><br />
compounds that will be very useful for a large-scale analysis <strong>of</strong> CaOx crystal growth modulators. © The Royal Society <strong>of</strong><br />
Chemistry <strong>2010</strong>.<br />
No.281<br />
Author (s) : Chutipongtanate, S., Thongboonkerd, V.<br />
Title : Renal tubular cell membranes inhibit growth but promote aggregation <strong>of</strong> calcium oxalate monohydrate crystals<br />
Source : Chemico-Biological Interactions, Volume 188, Issue 3, 5 December <strong>2010</strong>, Pages 421-426<br />
Keywords : Aggregation; Calcium oxalate; Crystal; Growth; Renal tubular cell; Stone<br />
<strong>Abstract</strong> : Cell membranes have been proposed to serve as promoters for calcium oxalate monohydrate (COM) kidney stone formation.<br />
However, direct evidence to demonstrate the modulatory effects <strong>of</strong> renal tubular cell membranes on COM crystals does not<br />
currently exist. We thus examined the effects <strong>of</strong> intact MDCK cells and their fragmented membranes on COM crystal growth,<br />
aggregation and transformation. COM crystals were generated in the absence (control) or presence <strong>of</strong> intact MDCK cells or<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
their membrane fragments. Intact MDCK cells and their membrane fragments significantly inhibited COM crystal growth (22.6%<br />
and 25.2% decreases in size, respectively) and significantly reduced COM total crystal mass (23.1% and 25.6% decreases,<br />
respectively). In contrast, both <strong>of</strong> them markedly promoted crystal aggregation (1.9-fold and 3.2-fold increases, respectively).<br />
Moreover, both intact cells and membrane fragments could transform COM to calcium oxalate dihydrate (COD) crystals.<br />
Finally, COM crystal growth inhibitory activities <strong>of</strong> both membrane forms were successfully confirmed by a<br />
spectrophotometric oxalate-depletion assay. Our data provide the first direct evidence to demonstrate the dual modulatory<br />
effects <strong>of</strong> MDCK membranes on COM crystals. Although growth <strong>of</strong> individual COM crystals was inhibited, their aggregation was<br />
promoted. These findings provide additional insights into the mechanisms <strong>of</strong> COM kidney stone formation. © Elsevier Ireland<br />
Ltd.<br />
No.282<br />
Author (s) : Chutipongtanate, S., Thongboonkerd, V.<br />
Title : Systematic comparisons <strong>of</strong> artificial urine formulas for in vitro cellular study<br />
Source : Analytical Biochemistry, Volume 402, Issue 1, July <strong>2010</strong>, Pages 110-112<br />
Document Type : Article<br />
Keywords : article, artificial urine, biocompatibility, cell strain, chemical composition, comparative study, in vitro<br />
study, kidney, nonhuman, priority journal, simulation, ultrafiltration, urinary tract, urine; Animals, Apoptosis, Cell Line, Cell<br />
Proliferation, Dogs, Humans, Kidney, Urine<br />
<strong>Abstract</strong> : Artificial urine (AU) is widely used for in vitro cellular study to simulate normal physiological environment <strong>of</strong> the kidney and<br />
urinary tract. However, some compositions <strong>of</strong> many <strong>of</strong> previously established AU formulas are out <strong>of</strong> their physiological<br />
ranges in normal human urine. Therefore, we established a new AU formula, named " AU-Siriraj," and then performed<br />
systematic comparisons <strong>of</strong> AU-Siriraj with other six previously established AU formulas, ultrafiltrated (UF) urine, and blankcontrol<br />
to determine their compatibility with MDCK cells. The data indicate that AU-Siriraj is the best, and AU-3 is the second<br />
best, AU formula for in vitro cellular study. © <strong>2010</strong> Elsevier Inc.<br />
No.283<br />
Author (s) : Chutipongtanate, S., Thongboonkerd, V.<br />
Title : Red Blood Cell Membrane Fragments but Not Intact Red Blood Cells Promote Calcium Oxalate Monohydrate Crystal Growth<br />
and Aggregation<br />
Source : JOURNAL OF UROLOGY, Volume: 184, Issue: 2, Pages: 743-749, DOI: 10.1016/j.juro.<strong>2010</strong>.03.107, Published: AUG <strong>2010</strong><br />
Document Type : Article<br />
Keywords : kidney; kidney calculi; erythrocytes; calcium oxalate; crystallization<br />
<strong>Abstract</strong> : Purpose: Cell membranes are thought to promote calcium oxalate kidney stone formation but to our knowledge the<br />
modulating effect <strong>of</strong> red blood cell membranes on calcium oxalate crystals has not been previously investigated. Thus, we<br />
examined the effects <strong>of</strong> red blood cell membrane fragments on calcium oxalate monohydrate and calcium oxalate dihydrate<br />
crystal growth and aggregation. Materials and Methods: Calcium oxalate monohydrate and calcium oxalate dihydrate crystals<br />
were treated with red blood cell membrane fragments or intact red blood cells from a healthy donor. Phase contrast<br />
microscopy was performed to evaluate crystal morphology and aggregation. We used ImageMaster (TM) 2D Platinum s<strong>of</strong>tware<br />
to evaluate crystal size and spectrophotometric oxalate depletion assay to monitor crystal growth. Results: Red blood cell<br />
membrane fragments had significant promoting activity on calcium oxalate monohydrate crystal growth with an<br />
approximately 75% increase in size and aggregation with an approximately 2.5-fold increase in aggregate number compared to<br />
the control without membrane fragments or cells. Approximately 50% <strong>of</strong> calcium oxalate monohydrate crystals were<br />
adhered by red blood cell membrane fragments. Intact red blood cells had no significant effect on calcium oxalate<br />
monohydrate crystal growth or aggregation but they could transform calcium oxalate monohydrate to calcium oxalate<br />
dihydrate crystals. Red blood cell membrane fragments and intact red blood cells had no effect on calcium oxalate<br />
dihydrate crystals. The promoting activity <strong>of</strong> red blood cell membrane fragments on calcium oxalate monohydrate crystal<br />
growth was successfully confirmed by spectrophotometric oxalate depletion assay. Conclusions: To our knowledge our data<br />
provide the first direct evidence that red blood cell membrane fragments are a promoting factor for calcium oxalate<br />
monohydrate crystal growth and aggregation. Thus, they may aggravate calcium oxalate stone formation.<br />
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No.284<br />
Author (s) : Cordell, H.J., Darlay, R., Charoen, P., Stewart, A., Gullett, A.M., Lambert, H.J., Malcolm, S., Feather, S.A., Goodship, T.H.J., Woolf,<br />
A.S., Kenda, R.B., Goodship, J.A.<br />
Title : Whole-genome linkage and association scan in primary, nonsyndromic vesicoureteric reflux<br />
Source : Journal <strong>of</strong> the American Society <strong>of</strong> Nephrology, Volume 21, Issue 1, January <strong>2010</strong>, Pages 113-123<br />
Document Type : Article<br />
Keywords : AGTR2 gene, article, gene, genetic association, genetic linkage, hnf1b gene, human, pax2 gene, population genetic<br />
parameters, priority journal, reflux nephropathy, ret gene, robo2 gene, single nucleotide polymorphism, upk3a<br />
gene, vesicoureteral reflux; Case-Control Studies, Data Interpretation, Statistical, Great Britain, Hepatocyte Nuclear Factor 1beta,<br />
Humans, Linkage (Genetics), Logistic Models, Membrane Glycoproteins, PAX2 Transcription Factor, Polymorphism, Single<br />
Nucleotide, Proto-Oncogene Proteins c-ret, Receptor, Angiotensin, Type 2, Receptors, Immunologic, Siblings, Slovenia, Vesico-<br />
Ureteral Reflux<br />
<strong>Abstract</strong> : Primary vesicoureteric reflux accounts for approximately 10% <strong>of</strong> kidney failure requiring dialysis or transplantation, and sibling<br />
studies suggest a large genetic component. Here, we report a whole-genome linkage and association scan in primary,<br />
nonsyndromic vesicoureteric reflux and reflux nephropathy. We used linkage and family-based association approaches to<br />
analyze 320 white families (661 affected individuals, generally from families with two affected siblings) from two populations<br />
(United Kingdom and Slovenian). We found modest evidence <strong>of</strong> linkage but no clear overlap with previous studies. We tested<br />
for but did not detect association with six candidate genes (AGTR2, HNF1B, PAX2, RET, ROBO2, and UPK3A). Family-based<br />
analysis detected associations with one single-nucleotide polymorphism (SNP) in the UK families, with three SNPs in the<br />
Slovenian families, and with three SNPs in the combined families. A case-control analysis detected associations with three<br />
additional SNPs. The results <strong>of</strong> this study, which is the largest to date investigating the genetics <strong>of</strong> reflux, suggest that major<br />
loci may not exist for this common renal tract malformation within European populations. Copyright © <strong>2010</strong> by the American<br />
Society <strong>of</strong> Nephrology.<br />
No.285<br />
Author (s) : Currier, J.R., Ngauy, V., de Souza, M.S., Ratto-Kim, S., Cox, J.H., Polonis, V.R., Earl, P., Moss, B., Peel, S., Slike, B., Sriplienchan, S.,<br />
Thongcharoen, P., Paris, R.M., Robb, M.L., Kim, J., Michael, N.L., Marovich, M.A.<br />
Title : Phase I safety and immunogenicity evaluation <strong>of</strong> MVA-CMDR, a multigenic, recombinant modified vaccinia ankara-HIV-1<br />
vaccine candidate<br />
Source : PLoS ONE, Volume 5, Issue 11, <strong>2010</strong>, Article number e13983<br />
Document Type : Article<br />
Keywords : chromium 51, gamma interferon, glycoprotein gp 120, Human immunodeficiency virus vaccine, modified vaccinia ankara<br />
chiang mai double recombinant vaccine, placebo, unclassified drug, virus envelope protein, Human immunodeficiency virus<br />
protein; adult, article, CD4+ T lymphocyte, cellular immunity, clinical trial, controlled clinical trial, controlled study, cytokine<br />
release, dose response, drug administration route, drug efficacy, drug megadose, drug safety, drug tolerability, enzyme linked<br />
immunosorbent assay, enzyme linked immunospot assay, female, human, human cell, Human immunodeficiency virus<br />
infection, humoral immunity, immunogenicity, lymphocyte proliferation, male, normal human, phase 1 clinical<br />
trial, randomized controlled trial, Thailand, United States, drug effect, fatigue, flow cytometry, gene vector, genetic<br />
recombination, genetics, headache, Human immunodeficiency virus 1, immunology, intradermal drug<br />
administration, intramuscular drug administration, methodology, multicenter study, nausea, vaccination, Vaccinia virus; Human<br />
immunodeficiency virus 1; Adult, AIDS Vaccines, Dose-Response Relationship, Drug, Enzyme-Linked Immunosorbent<br />
Assay, Fatigue, Female, Flow Cytometry, Genetic Vectors, Headache, HIV Infections, HIV-1, Human Immunodeficiency Virus<br />
Proteins, Humans, Immunity, Cellular, Immunity, Humoral, Injections, Intradermal, Injections,<br />
Intramuscular, Male, Nausea, Recombination, Genetic, Thailand, United States, Vaccination, Vaccinia virus<br />
<strong>Abstract</strong> : Background: We conducted a Phase I randomized, dose-escalation, route-comparison trial <strong>of</strong> MVA-CMDR, a candidate HIV-1<br />
vaccine based on a recombinant modified vaccinia Ankara viral vector expressing HIV-1 genes env/gag/pol. The HIV sequences<br />
were derived from circulating recombinant form CRF01_AE, which predominates in Thailand. The objective was to evaluate<br />
safety and immunogenicity <strong>of</strong> MVA-CMDR in human volunteers in the US and Thailand. Methodology/Principal Findings: MVA-<br />
CMDR or placebo was administered intra-muscularly (IM; 107 or 108 pfu) or intradermally (ID; 106 or 107 pfu) at months 0, 1<br />
and 3, to 48 healthy volunteers at low risk for HIV-1 infection. Twelve volunteers in each dosage group were randomized to<br />
receive MVA-CMDR or placebo (10:2). Volunteers were actively monitored for local and systemic reactogenicity and adverse<br />
events post vaccination. Cellular immunogenicity was assessed by a validated IFNγ Elispot assay, an intracellular cytokine<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
staining assay, lymphocyte proliferation and a 51Cr-release assay. Humoral immunogenicity was assessed by ADCC for gp120<br />
and binding antibody ELISAs for gp120 and p24. MVA-CMDR was safe and well tolerated with no vaccine related serious<br />
adverse events. Cell-mediated immune responses were: (i) moderate in magnitude (median IFNγ Elispot <strong>of</strong> 78 SFC/106 PBMC<br />
at 108 pfu IM), but high in response rate (70% 51Cr-release positive; 90% Elispot positive; 100% ICS positive, at 108 pfu IM); (ii)<br />
predominantly HIV Env-specific CD4+ T cells, with a high proliferative capacity and durable for at least 6 months (100% LPA<br />
response rate by the IM route); (iv) dose- and routedependent with 108 pfu IM being the most immunogenic treatment.<br />
Binding antibodies against gp120 and p24 were detectable in all vaccination groups with ADCC capacity detectable at the<br />
highest dose (40% positive at 108 pfu IM). Conclusions/Significance: MVA-CMDR delivered both intramuscularly and<br />
intradermally was safe, well-tolerated and elicited durable cell-mediated and humoral immune responses.<br />
No.286<br />
Author (s) : Dajpratham, P., Ploypetch, T., Kiattavorncharoen, S., Boonsiriseth, K.<br />
Title : Prevalence and associated factors <strong>of</strong> musculoskeletal pain among the dental personnel in a dental school<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 6, June <strong>2010</strong>, Pages 714-721<br />
Document Type : Article<br />
Keywords : Dental assistant; Dentist; Musculoskeletal; Pain; Prevalence<br />
<strong>Abstract</strong> : Objective: To study the prevalence and associated factors <strong>of</strong> musculoskeletal (MS) pain among the dental personnel. In<br />
addition, impacts and treatment <strong>of</strong> MS pain were reported. Material and Method: Random sampling <strong>of</strong> 390 participants from<br />
the name lists <strong>of</strong> dental personnel working in each department. Self-administered questionnaires were equally distributed to<br />
three groups <strong>of</strong> dental personnel namely clinical instructors, postgraduate students, and dental assistants. The present study<br />
was conducted as a survey in the Faculty <strong>of</strong> Dentistry, <strong>Mahidol</strong> <strong>University</strong>, Bangkok between December 2008 and January<br />
2009. Results: Three hundred and ninety questionnaires were delivered and 164 questionnaires were returned (response rate<br />
42.5%). The participants with MS pain were 32 clinical instructors (20.3%), 52 postgraduate students (32.9%), and 74 dental<br />
assistants (46.8%). Their mean age was 33.0 ± 9.1 years old. The MS pain found respectively was shoulder pain 72.2% (n =<br />
114), neck pain 70.3% (n = 111), and low back pain 50.6% (n = 80). The participants with shoulder and neck pain were<br />
combined and defined as cervicobrachial pain. The associated factor <strong>of</strong> cervicobrachial pain was working status. Being a<br />
clinical instructor and postgraduate student were associated with cervicobrachial pain with OR being 4.7 [1.3, 7.1] and 4.6 [1.6,<br />
13.4], respectively. The impacts <strong>of</strong> MS pain among the dental personnel included usage <strong>of</strong> pain relieving medication (34.8%),<br />
seeking medical evaluation (32.3%), reduction in working hours (27.2%), difficulty sleeping (22.8%), and work absence (10.8%),<br />
respectively. The treatments <strong>of</strong> MS pain utilized to alleviate those impacts were Thai traditional massage (51.9%), medication<br />
(28.5%), physical therapy (15.8%), acupuncture (7.6%), and alternative medicine (4.4%), respectively. Conclusion:<br />
Cervicobrachial pain was the most prevalent MS pain among the dental personnel and working status was associated with<br />
their MS pain problems. The impact <strong>of</strong> MS pain was predominantly usage <strong>of</strong> pain relieving medication. Thai traditional<br />
massage was the most utilized treatment.<br />
No.287<br />
Author (s) : Danpornprasert, P., Foongladda, S., Tscheikuna, J.<br />
Title : Impact <strong>of</strong> bronchoalveolar lavage galactomannan on the outcome <strong>of</strong> patients at risk for invasive pulmonary aspergillosis.<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand = Chotmaihet thangphaet, Volume 93 Suppl 1, January <strong>2010</strong>, Pages S86-93<br />
Document Type : Article<br />
Keywords : antifungal agent, galactomannan, mannan; adolescent, adult, aged, article, blood, bronchoscopy, chemistry, female, follow<br />
up, human, immunocompromised patient, lung aspergillosis, lung lavage, lung mycosis, male, microbiology, middle<br />
aged, mortality, prospective study, risk, sensitivity and specificity, survival rate, treatment outcome;<br />
Adolescent, Adult, Aged, Antifungal Agents, Bronchoalveolar Lavage Fluid, Bronchoscopy, Female, Follow-Up<br />
Studies, Humans, Immunocompromised Host, Invasive Pulmonary Aspergillosis, Lung Diseases, Fungal, Male, Mannans, Middle<br />
Aged, Prospective Studies, Risk, Sensitivity and Specificity, Survival Rate, Treatment Outcome, Young Adult<br />
<strong>Abstract</strong> : BACKGROUND: Invasive pulmonary aspergillosis (IPA) is an important cause <strong>of</strong> morbidity and mortality among<br />
immunocompromised patients especially in neutropenic and patients treated with immunosuppressive drugs. New diagnostic<br />
tools have been developed to improve treatment and outcome. Compared with serum galactomannan, bronchoalveolar<br />
lavage galactomannan (BAL GM) detection has higher sensitivity (81% vs. 71%) and comparable specificity (87.6% vs. 89%). No<br />
study has correlated this test result to clinical outcome. MATERIAL AND METHOD: A prospective non-randomised study was<br />
conducted from March to December 2008 in adult patients who were suspected to have invasive pulmonary aspergillosis<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
(IPA). Serum galactomannan levels were measured and bronchoscopy was performed to obtained BAL fluid for direct<br />
examination, culture, and measurement <strong>of</strong> galactomannan level. Response to treatment and mortality within 6-weeks <strong>of</strong><br />
follow-up were compared between positive and negative BAL GM groups. Factors influencing outcome were also analysed.<br />
RESULTS: There were 30 patients with 3 probable, 11 possible and 17 no IPA. Other causative organisms can be identified in 8<br />
<strong>of</strong> 17 patients in the no IPA group. Overall, BAL GM at the 0.5 cut-<strong>of</strong>f yielded a 46% positive result compared with 13% <strong>of</strong><br />
serum GM (p = 0.005). There was no significant difference in positive result between BAL GM at 1.0 cut-<strong>of</strong>f and serum GM. By<br />
using BAL GM as a mycological criteria, 54% <strong>of</strong> possible IPA was upgraded to probable IPA. Neither BAL GM nor serum GM<br />
results were associated with clinical response and mortality. Recovery <strong>of</strong> neutropenia was the only factor associated with<br />
response to treatment and outcome (p = 0.003). CONCLUSION: BAL GM detection has a higher positive rate than serum GM in<br />
patients at risk for IPA. It is helpful in diagnosis and categorization <strong>of</strong> IPA, but its impact on clinical outcome cannot be<br />
demonstrated in this study.<br />
No.288<br />
Author (s) : Daung-nkern, J., Vattanaviboon, P., Mongkolsuk, S.<br />
Title : Inactivation <strong>of</strong> nfuA enhances susceptibility <strong>of</strong> Pseudomonas aeruginosa to fluoroquinolone antibiotics<br />
Source : Journal <strong>of</strong> Antimicrobial Chemotherapy, Volume 65, Issue 8, 4 June <strong>2010</strong>, Article number dkq194, Pages 1831-1832<br />
Document Type : Letter<br />
Keywords : Fluoroquinolones; Iron-sulphur clusters; Nfua; P. aeruginosa<br />
<strong>Abstract</strong> : –<br />
No.289<br />
Author (s) : Decker, M.R., McCauley, H.L., Phuengsamran, D., Janyam, S., Seage III, G.R., Silverman, J.G.<br />
Title : Violence victimisation, sexual risk and sexually transmitted infection symptoms among female sex workers in Thailand<br />
Source : Sexually Transmitted Infections, Volume 86, Issue 3, June <strong>2010</strong>, Pages 236-240<br />
Document Type : Article<br />
Keywords : adolescent, adult, article, clinical assessment, comparative study, condom, demography, female, health<br />
survey, human, incidence, informed consent, major clinical study, occupational exposure, prevalence, priority<br />
journal, prostitution, risk factor, sexual crime, sexual health, sexually transmitted disease, symptom, Thailand, violence, work<br />
environment; Adolescent, Adult, Crime Victims, Female, Humans, Prevalence, Prostitution, Sexually Transmitted<br />
Diseases, Thailand, Unsafe Sex, Violence, Young Adult<br />
<strong>Abstract</strong> : Background/Objectives: Commercial sex work is a primary context for heterosexual HIV/AIDS transmission. Violence<br />
victimisation is considered to compromise women's ability to protect against HIV and other sexually transmitted infections<br />
(STI); little research has investigated violence as it relates to sexual risk and STI among female sex workers (FSW). This study<br />
sought to compare sexual risk and STI symptoms among FSW based on recent violence exposure. Methods: Data from 815<br />
FSW in Thailand were used to assess the prevalence <strong>of</strong> physical or sexual violence within the context <strong>of</strong> sex work, and<br />
associations <strong>of</strong> victimisation with sexual risk and STI symptoms. Results: Approximately one in seven FSW (14.6%) had<br />
experienced violence in the week before the survey. Compared with their unexposed counterparts, FSW exposed to violence<br />
demonstrated a greater risk <strong>of</strong> condom failure (19.6% vs 12.3%, ARR 1.92, 95% CI 1.24 to 2.95) and client condom refusal<br />
(85.7% vs 69.0%, ARR 1.24, 95% CI 1.14 to 1.35). In analyses adjusted for sexual risk, violence related to STI symptoms<br />
collectively (ARR 1.11, 95% CI 1.02 to 1.21) and genital lesions as an individual STI symptom (ARR 1.78, 95% CI 1.20 to 2.66).<br />
Conclusion: Physical and sexual violence against FSW in Thailand appears to be common, with women experiencing such<br />
violence demonstrating diminished capacity for STI/HIV harm reduction and greater prevalence <strong>of</strong> STI symptoms. Efforts to<br />
reduce violence towards this vulnerable population must be prioritised, as a means <strong>of</strong> protecting the health and wellbeing <strong>of</strong><br />
FSW, and as a key component <strong>of</strong> STI/HIV prevention and control.<br />
No.290<br />
Author (s) : Dejnirattisai, W., Jumnainsong, A., Onsirisakul, N., Fitton, P., Vasanawathana, S., Limpitikul, W., Puttikhunt, C., Edwards, C.,<br />
Duangchinda, T., Supasa, S., Chawansuntati, K., Malasit, P., Mongkolsapaya, J., Screaton, G.<br />
Title : Cross-reacting antibodies enhance dengue virus infection in humans<br />
Source : Science, Volume 328, Issue 5979, 7 May <strong>2010</strong>, Pages 745-748<br />
Document Type : Article<br />
105
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : cross reacting antibody, dengue vaccine, Fc receptor, human monoclonal antibody, membrane protein, protein precursor;<br />
antibody, dengue fever, disease resistance, disease spread, vaccine, virus; antibody blood level, antibody dependent<br />
enhancement, antibody response, article, controlled study, dengue, Dengue virus, disease severity, drug<br />
design, human, human cell, nonhuman, priority journal, protein cleavage, serotype, surface property;<br />
Aedes, Animals, Antibodies, Monoclonal, Antibodies, Neutralizing, Antibodies, Viral, Antibody-Dependent<br />
Enhancement, Antigens, Viral, Cell Line, Cross Reactions, Dengue, Dengue Vaccines, Dengue Virus, Encephalitis Virus,<br />
Japanese, Humans, Immune Evasion, Monocytes, Receptors, Fc, Serotyping, U937 Cells, Viral Envelope Proteins, Viral Matrix<br />
Proteins, Virus Replication; Dengue virus, Dengue virus group<br />
<strong>Abstract</strong> : Dengue virus co-circulates as four serotypes, and sequential infections with more than one serotype are common. One<br />
hypothesis for the increased severity seen in secondary infections is antibodydependent enhancement (ADE) leading to<br />
increased replication in Fc receptor-bearing cells. In this study, we have generated a panel <strong>of</strong> human monoclonal antibodies<br />
to dengue virus. Antibodies to the structural precursor-membrane protein (prM) form a major component <strong>of</strong> the response.<br />
These antibodies are highly cross-reactive among the dengue virus serotypes and, even at high concentrations, do not<br />
neutralize infection but potently promote ADE. We propose that the partial cleavage <strong>of</strong> prM from the viral surface reduces<br />
the density <strong>of</strong> antigen available for viral neutralization, leaving dengue viruses susceptible to ADE by antibody to prM, a<br />
finding that has implications for future vaccine design.<br />
No.291<br />
Author (s) : Dejthevaporn, C.; Waisayarat, J.<br />
Title : Mitochondrial cytopathy presenting with late onset irritative myopathy with acute respiratory failure: The important role <strong>of</strong><br />
muscle biopsy<br />
Source : VALUE IN HEALTH, Volume: 13, Issue: 7, Pages: A548-A549, Published: NOV <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.292<br />
Author (s) : Dewasurendra, R.L.; Suriyaphol, P.; Fernando, D.; Sereejaitham, P.; Karunaweera, N.D.<br />
Title : GENETIC MARKERS AND RISK OF MALARIA INFECTIONS: GENETIC-EPIDEMIOLOGY STUDY IN A LOW MALARIA ENDEMIC AREA OF<br />
SRI LANKA<br />
Source : VALUE IN HEALTH, Volume: 13, Issue: 7, Pages: A548-A549, Published: NOV <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.293<br />
Author (s) : DeWeaver, M.A., Shannon, R.<br />
Title : Waning vigilance and the disposition effect: Evidence from Thailand on individual investor decision making<br />
Source : Journal <strong>of</strong> Socio-Economics, Volume 39, Issue 1, January <strong>2010</strong>, Pages 18-23<br />
Document Type : Article<br />
Keywords : Cognitive dissonance; Disposition effect; Entrapment; Investor behavior; Vigilant information processing<br />
<strong>Abstract</strong> : We argue that existing explanations for the stock-market investor's disposition to "ride losers too long" are unsatisfactory<br />
because they abstract from any role for information processing. We propose instead that the disposition effect is a special<br />
case <strong>of</strong> "waning vigilance:" investors pay less attention to new information and analysis when making decisions about loss<br />
makers and are therefore slower to sell them when arguments in favor <strong>of</strong> holding cease to be valid. Results from a Thai<br />
individual investor survey are presented as empirical evidence in support <strong>of</strong> the hypothesis that vigilance is reduced following<br />
losses. © 2009 Elsevier Inc. All rights reserved.<br />
No.294<br />
Author (s) : Dharmasaroja, P.<br />
Title : Signal intensity loss on t2-weighted gradient-recalled echo magnetic resonance images in the basal ganglia in a patient with<br />
chronic hepatic encephalopathy<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Source : Neurologist, Volume 16, Issue 4, July <strong>2010</strong>, Pages 265-268<br />
Document Type : Article<br />
Keywords : basal ganglia; brain MRI; extrapyramidal; liver; manganese<br />
<strong>Abstract</strong> : Background: Bilaterally symmetrical hyperintensity on T1-weighted magnetic resonance images (MRIs) without abnormalities<br />
on T2-weighted images in the basal ganglia is described in patients with chronic liver disease. Manganese, which escapes<br />
hepatic clearance because <strong>of</strong> a portosystemic shunt or liver dysfunction, is thought to be involved in alterations <strong>of</strong> signal<br />
intensity on MRIs, and exerts neurotoxicity, which results in neuropsychiatric disturbances including extrapyramidal symptoms.<br />
Rationales and case: Currently, reports evaluating interpretations <strong>of</strong> hyperintensity on T1- and normal intensity on T2weighted<br />
images still provide conflicting results. T2-weighted gradient-recalled echo (GRE) MR imaging is dependent on<br />
magnetic susceptibility effect and is highly sensitive to static magnetic field inhomogeneity. Field distortions caused by<br />
material with high magnetic susceptibility induce signal intensity loss, resulting in typical signal intensity voids. This article<br />
describes asymmetric signal intensity loss on T2-weighted GRE MRIs in the globus pallidus in a patient with chronic hepatitis C<br />
infection presenting with a gradual onset <strong>of</strong> lethargy, dysarthria, and gait instability; whereas T1-weighted MRIs showed<br />
symmetrical hyperintensity in this region and the midbrain and T2-weighted images showed normal signal intensity.<br />
Conclusion: T2-weighted GRE MR imaging should be included in brain MR imaging studies <strong>of</strong> patients with chronic liver disease<br />
presenting with extrapyramidal symptoms for better localization <strong>of</strong> the lesions. © <strong>2010</strong> by Lippincott Williams & Wilkins.<br />
No.295<br />
Author (s) : Dhitavat, J.; Charoenwatanachokchai, A.; Kongsin, S.; Kaewkungwal, J.; Ruengkris, T.; Bussaratid, V; Pitisuttithum, P.<br />
Title : BURDEN AND MEDICAL COSTS OF ANOGENITAL WARTS IN BANGKOK, THAILAND<br />
Source : VALUE IN HEALTH, Volume: 13, Issue: 7, Pages: A548-A549, Published: NOV <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.296<br />
Author (s) : Dimmock, S.G., Kouwenberg, R.<br />
Title : Loss-aversion and household portfolio choice<br />
Source : Journal <strong>of</strong> Empirical Finance, Volume 17, Issue 3, June <strong>2010</strong>, Pages 441-459<br />
Document Type : Article<br />
Keywords : Limited participation; Loss-aversion; Portfolio choice; Prospect theory; Stock market participation<br />
<strong>Abstract</strong> : In this paper we empirically test if loss-aversion affects household participation in equity markets, household allocations to<br />
equity, and household allocations between mutual funds and individual stocks. Using household survey data, we obtain<br />
direct measures <strong>of</strong> each surveyed household's loss-aversion coefficient from questions involving hypothetical pay<strong>of</strong>fs. We find<br />
that higher loss-aversion is associated with a lower probability <strong>of</strong> participation. We also find that higher loss-aversion reduces<br />
the probability <strong>of</strong> direct stockholding by significantly more than the probability <strong>of</strong> owning mutual funds. After controlling for<br />
sample selection we do not find a relationship between loss-aversion and portfolio allocations to equity. © 2009 Elsevier B.V.<br />
No.297<br />
Author (s) : Disayabutr, S., Tscheikuna, J., Nana, A.<br />
Title : The endobronchial ultrasound-guided transbronchial lung biopsy in peripheral pulmonary lesions.<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand = Chotmaihet thangphaet, Volume 93 Suppl 1, January <strong>2010</strong>, Pages S94-101<br />
Document Type : Article<br />
Keywords : adult, aged, article, biopsy, bronchoscope, bronchoscopy, cross-sectional study, echography, endoscopic<br />
echography, female, human, instrumentation, lung tumor, male, methodology, middle aged, multiple pulmonary<br />
nodules, pathology, prospective study; Adult, Aged, Aged, 80 and over, Biopsy, Bronchoscopes, Bronchoscopy, Cross-Sectional<br />
Studies, Endosonography, Female, Humans, Lung Neoplasms, Male, Middle Aged, Multiple Pulmonary Nodules, Prospective<br />
Studies, Young Adult<br />
<strong>Abstract</strong> : BACKGROUND: Peripheral pulmonary lesions are technically challenging with conventional bronchoscopy in obtaining tissue<br />
diagnosis. The recently developed small-caliber ultrasonographic probe can be introduced via the working channel <strong>of</strong> a<br />
flexible bronchoscope to localize peripheral pulmonary lesions (PPLs) prior to transbronchial lung biopsy (TBLB). The<br />
endobronchial ultrasound-guided transbronchial lung biopsy (EBUS-TBLB) is a new diagnostic method for the diagnosis <strong>of</strong><br />
107
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
pulmonary lesions in our center. OBJECTIVE: To evaluate the diagnostic yield <strong>of</strong> EBUS-TBLB in pulmonary lesions. STUDY<br />
DESIGN: A prospective cross-sectional study. MATERIAL AND METHOD: We enrolled 152 patients with pulmonary lesions that<br />
were beyond the segmental bronchus and had no evidence <strong>of</strong> endobronchial lesion, who underwent bronchoscopy in our<br />
center. With EBUS assisted, transbronchial lung biopsy was performed after localizing and measuring distance from the tip <strong>of</strong><br />
bronchoscope to the lesion. The diagnostic yield was calculated. RESULTS: The pulmonary lesions were visible on EBUS<br />
image in 98.7% <strong>of</strong> cases. The overall diagnostic yield <strong>of</strong> EBUS-TBLB was 66.4%. The diagnostic yield in the infiltrative and mass<br />
lesions were 86.4% and 63.1%, respectively. The lesions which EBUS probe located within it were diagnosed by EBUS-TBLB<br />
about 74.8%. The benign and malignant lesions were diagnosed by EBUS-TBLB about 81.1% and 58.6%, respectively. The<br />
average EBUS time was 3.55 +/- 2.29 minutes. No complication <strong>of</strong> EBUS and transbronchial lung biopsy were observed in this<br />
study. CONCLUSION: EBUS-TBLB is a safe procedure for diagnosing pulmonary lesions. Our results indicate that the EBUS-TBLB<br />
improves the diagnostic yield compared to conventional brochoscopy.<br />
No.298<br />
Author (s) : Disthabanchong, S., Treeruttanawanich, A.<br />
Title : Oral sodium bicarbonate improves thyroid function in predialysis chronic kidney disease<br />
Source : American Journal <strong>of</strong> Nephrology, Volume 32, Issue 6, December <strong>2010</strong>, Pages 549-556<br />
Document Type : Article<br />
Keywords : Acidosis; Chronic kidney disease; Renal failure; Sodium bicarbonate; Thyroid; Thyroid-stimulating<br />
hormone; Thyroxine; Triiodothyronine<br />
<strong>Abstract</strong> : Background/Aims: Metabolic acidosis (MA) in chronic kidney disease (CKD) associates with protein energy malnutrition,<br />
osteoporosis, abnormal endocrine function and increased mortality. Oral sodium bicarbonate has been shown to improve<br />
nutritional status and preserve renal function in CKD. Depressed thyroid function has been described in CKD and was believed<br />
to be related to MA. This is a prospective randomized study that examined the effect <strong>of</strong> oral sodium bicarbonate on thyroid<br />
function in predialysis CKD with MA. Methods: Predialysis CKD patients with serum total CO2 ≤22 mM were randomized into<br />
two groups. The treatment group received increasing dose <strong>of</strong> oral sodium bicarbonate until serum total CO2 was ≥24 mM.<br />
Control patients were kept on the same medications. Thyroid function tests were measured at baseline and again after 8-12<br />
weeks. Results: All patients had a glomerular filtration rate <35 ml/min/1.73 m2. Serum total CO2 increased significantly in<br />
the treatment group and was unchanged in the control group. At baseline, over half <strong>of</strong> the patients had T3 below the lower<br />
limit <strong>of</strong> normal. At study completion, free T3 declined further in the control group, whereas free T3, total T3, free T4 and TSH<br />
rose significantly in the treatment group. Percentage changes <strong>of</strong> total CO2 from baseline were strongly associated with the<br />
changes <strong>of</strong> T3 parameters. Glomerular filtration rate was maintained in the treatment group but declined significantly in the<br />
control group. Conclusion: Oral sodium bicarbonate, through correction <strong>of</strong> MA, improved thyroid function in predialysis CKD.<br />
Copyright © <strong>2010</strong> S. Karger AG, Basel.<br />
No.299<br />
Author (s) : Ditcharoen, N., Naruedomkul, K., Cercone, N.<br />
Title : SignMT: An alternative language learning tool<br />
Source : Computers and Education, Volume 55, Issue 1, August <strong>2010</strong>, Pages 118-130<br />
Document Type : Article<br />
Keywords : Computer-assisted language learning; Computer-mediate communication; Interactive learning environments; Media in<br />
education; Sign language machine translation<br />
<strong>Abstract</strong> : Learning a second language is very difficult, especially, for the disabled; the disability may be a barrier to learn and to utilize<br />
information written in text form. We present the SignMT, Thai sign to Thai machine translation system, which is able to<br />
translate from Thai sign language into Thai text. In the translation process, SignMT takes into account the differences between<br />
Thai and Thai sign language in terms <strong>of</strong> both syntax and semantic to ensure the accuracy <strong>of</strong> translation. SignMT was designed<br />
to be not only an automatic interpreter but also a language learning tool. It provides meaning <strong>of</strong> each word in both text and<br />
image forms which is easy to understand by the deaf. The grammar information and the order <strong>of</strong> the sentence are presented<br />
in order to help the deaf in learning Thai, their second language. With SignMT, deaf students are less dependent on a teacher,<br />
have more freedom to experiment with their own language, and improve their knowledge and learning skill. In our<br />
experiment, SignMT was implemented to translate sentences/phrases which were collected from different sources including<br />
textbooks, cartoons, bedtime story, and newspapers. SignMT was tested and evaluated in terms <strong>of</strong> the translation accuracy<br />
108
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
and user satisfaction. The evaluation results show that the translation accuracy is acceptable, and it satisfies the users' needs.<br />
© <strong>2010</strong> Elsevier Ltd. All rights reserved.<br />
No.300<br />
Author (s) : Domrongkitchaiporn, S., Disthabanchong, S., Cheawchanthanakij, R., Niticharoenpong, K., Stitchantrakul, W., Charoenphandhu,<br />
N., Krishnamra, N.<br />
Title : Oral phosphate supplementation corrects hypophosphatemia and normalizes plasma FGF23 and 25-hydroxyvitamin D3 levels<br />
in women with chronic metabolic acidosis<br />
Source : Experimental and Clinical Endocrinology and Diabetes, Volume 118, Issue 2, <strong>2010</strong>, Pages 105-112<br />
Document Type : Article<br />
Keywords : Acidemia; Ammonium chloride; Fibroblast growth factor 23 (FGF23); Parathyroid hormone<br />
<strong>Abstract</strong> : Background: Chronic metabolic acidosis (CMA) is known to induce renal phosphate wasting and hypophosphatemia by<br />
enhancing bone resorption and inhibiting renal phosphate reabsorption. However, nothing is known regarding changes in the<br />
plasma levels <strong>of</strong> phosphate-regulating hormones during CMA, especially in humans with normal kidney function. Methods:<br />
Fifteen healthy Thai female volunteers were given NH4Cl orally for 7 days to induce CMA with or without oral phosphate<br />
supplementation. Blood and 24-h urine specimens were collected prior to and after CMA induction. Plasma concentrations<br />
and fractional excretion <strong>of</strong> calcium and inorganic phosphate as well as plasma levels <strong>of</strong> fibroblast growth factor (FGF) 23,<br />
25(OH)D3, 1,25(OH) 2D3 and intact parathyroid hormone (iPTH) were determined. Results: CMA led to hypophosphatemia and<br />
hypocalcemia with increases in the fractional excretion <strong>of</strong> calcium and phosphate. Plasma concentrations <strong>of</strong> FGF23, 25(OH)D3<br />
and iPTH were decreased, whereas that <strong>of</strong> 1,25(OH) 2D3 was increased. After oral phosphate supplementation, CMA-induced<br />
changes in the concentrations <strong>of</strong> the studied ions, FGF23 and 25(OH)D3, but not those <strong>of</strong> 1,25(OH)2D3 and iPTH, were<br />
diminished. Conclusions: The CMA-induced hypophosphatemia was likely to initiate a negative feedback response, thereby<br />
leading to reduction in the plasma levels <strong>of</strong> hyperphosphaturic hormones, FGF23 and PTH. An increase in the plasma<br />
1,25(OH)2D3 level, despite diminishing 25(OH)D 3 storage pool, may help enhance the intestinal phosphate absorption. Oral<br />
phosphate supplementation abolished the effects <strong>of</strong> CMA on FGF23 and 25(OH)D3 levels, suggesting that the plasma<br />
phosphate concentration is the primary regulator <strong>of</strong> the plasma levels <strong>of</strong> these hormones during CMA. © J. A. Barth Verlag in<br />
Georg Thieme Verlag KG Stuttgart - New York.<br />
No.301<br />
Author (s) : Dondorp, A.M., Fanello, C.I., Hendriksen, I.C., Gomes, E., Seni, A., Chhaganlal, K.D., Bojang, K., Olaosebikan, R., Anunobi, N.,<br />
Maitland, K., Kivaya, E., Agbenyega, T., Nguah, S.B., Evans, J., Gesase, S., Kahabuka, C., Mtove, G., Nadjm, B., Deen, J., Mwanga-<br />
Amumpaire, J., Nansumba, M., Karema, C., Umulisa, N., Uwimana, A., Mokuolu, O.A., Adedoyin, O.T., Johnson, W.B., Tshefu,<br />
A.K., Onyamboko, M.A., Sakulthaew, T., Ngum, W.P., Silamut, K., Stepniewska, K., Woodrow, C.J., Bethell, D., Wills, B., Oneko,<br />
M., Peto, T.E., Von Seidlein, L., Day, N.P., White, N.J.<br />
Title : Artesunate versus quinine in the treatment <strong>of</strong> severe falciparum malaria in African children (AQUAMAT): An open-label,<br />
randomised trial<br />
Source : The Lancet, Volume 376, Issue 9753, 13 November <strong>2010</strong>, Pages 1647-1657<br />
Document Type : Article<br />
Keywords : artemether plus benflumetol, artesunate, quinine; adolescent, Africa, article, child, clinical trial, coma, controlled clinical<br />
trial, controlled study, convulsion, drug tolerability, female, human, hypersensitivity reaction, hypoglycemia, loading drug<br />
dose, malaria falciparum, male, mortality, multicenter study, neurologic disease, outcome assessment, pediatrics, preschool<br />
child, priority journal, randomized controlled trial, rash, school child, treatment outcome, unspecified side effect; Africa South<br />
<strong>of</strong> the Sahara, Antimalarials, Artemisinins, Child, Preschool, Female, Humans, Infant, Kaplan-Meier Estimate, Malaria,<br />
Falciparum, Male, Quinine, Survival Rate<br />
<strong>Abstract</strong> : Severe malaria is a major cause <strong>of</strong> childhood death and <strong>of</strong>ten the main reason for paediatric hospital admission in sub-<br />
Saharan Africa. Quinine is still the established treatment <strong>of</strong> choice, although evidence from Asia suggests that artesunate is<br />
associated with a lower mortality. We compared parenteral treatment with either artesunate or quinine in African children<br />
with severe malaria. This open-label, randomised trial was undertaken in 11 centres in nine African countries. Children (
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
treat. This trial is registered, number ISRCTN50258054. 5425 children were enrolled; 2712 were assigned to artesunate and<br />
2713 to quinine. All patients were analysed for the primary outcome. 230 (8·5) patients assigned to artesunate treatment died<br />
compared with 297 (10·9) assigned to quinine treatment (odds ratio [OR] stratified for study site 0·75, 95 CI 0·63-0·90; relative<br />
reduction 22·5, 95 CI 8·1-36·9; p=0·0022). Incidence <strong>of</strong> neurological sequelae did not differ significantly between groups, but<br />
the development <strong>of</strong> coma (65/1832 [3·5] with artesunate vs 91/1768 [5·1] with quinine; OR 0·69 95 CI 0·49-0·95; p=0·0231),<br />
convulsions (224/2712 [8·3] vs 273/2713 [10·1]; OR 0·80, 0·66-0·97; p=0·0199), and deterioration <strong>of</strong> the coma score (166/2712<br />
[6·1] vs 208/2713 [7·7]; OR 0·78, 0·64-0·97; p=0·0245) were all significantly less frequent in artesunate recipients than in<br />
quinine recipients. Post-treatment hypoglycaemia was also less frequent in patients assigned to artesunate than in those<br />
assigned to quinine (48/2712 [1·8] vs 75/2713 [2·8]; OR 0·63, 0·43-0·91; p=0·0134). Artesunate was well tolerated, with no<br />
serious drug-related adverse effects. Artesunate substantially reduces mortality in African children with severe malaria. These<br />
data, together with a meta-analysis <strong>of</strong> all trials comparing artesunate and quinine, strongly suggest that parenteral artesunate<br />
should replace quinine as the treatment <strong>of</strong> choice for severe falciparum malaria worldwide. The Wellcome Trust. © <strong>2010</strong><br />
Elsevier Ltd.<br />
No.302<br />
Author (s) : Dondorp, A.M., Yeung, S., White, L., Nguon, C., Day, N.P.J., Socheat, D., Von Seidlein, L.<br />
Title : Artemisinin resistance: Current status and scenarios for containment<br />
Source : Nature Reviews Microbiology, Volume 8, Issue 4, April <strong>2010</strong>, Pages 272-280<br />
Document Type : Review<br />
Keywords : 8 aminoquinoline derivative, amodiaquine, amodiaquine plus artesunate, arteether, artemether, artemether plus<br />
benflumetol, artemisinin, artemisinin derivative, artesunate, atovaquone plus<br />
proguanil, benflumetol, chloroquine, dihydroartemisinin, dihydroartemisinin derivative, dihydroartemisinin plus<br />
piperaquine, fansidar, mefloquine, primaquine, pyronaridine, quinine; antimalarial drug resistance, brain<br />
malaria, Cambodia, disease surveillance, drug exposure, drug half life, drug structure, early diagnosis, endemic<br />
disease, human, malaria, malaria falciparum, mathematical model, monotherapy, phenotype, priority journal, review, single<br />
drug dose, Thailand, vector control; Antimalarials, Artemisinins, Cambodia, Disease Outbreaks, Drug Resistance, Malaria,<br />
Falciparum, Plasmodium falciparum, Thailand; Plasmodium falciparum<br />
<strong>Abstract</strong> : Artemisinin combination therapies are the first-line treatments for uncomplicated Plasmodium falciparum malaria in most<br />
malaria-endemic countries. Recently, partial artemisinin-resistant P. falciparum malaria has emerged on the<br />
Cambodiaĝ€"Thailand border. Exposure <strong>of</strong> the parasite population to artemisinin monotherapies in subtherapeutic doses for<br />
over 30 years, and the availability <strong>of</strong> substandard artemisinins, have probably been the main driving force in the selection <strong>of</strong><br />
the resistant phenotype in the region. A multifaceted containment programme has recently been launched, including early<br />
diagnosis and appropriate treatment, decreasing drug pressure, optimising vector control, targeting the mobile population,<br />
strengthening management and surveillance systems, and operational research. Mathematical modelling can be a useful tool<br />
to evaluate possible strategies for containment. © <strong>2010</strong> Macmillan Publishers Limited. All rights reserved.<br />
No.303<br />
Author (s) : Douglas, N.M., Anstey, N.M., Angus, B.J., Nosten, F., Price, R.N.<br />
Title : Artemisinin combination therapy for vivax malaria<br />
Source : The Lancet Infectious Diseases, Volume 10, Issue 6, June <strong>2010</strong>, Pages 405-416<br />
Document Type : Review<br />
Keywords : amodiaquine, amodiaquine plus artesunate, antimalarial agent, artemether, artemether plus<br />
benflumetol, artemisinin, artesunate, artesunate plus mefloquine, artesunate plus<br />
sulfadoxine, benflumetol, chloroquine, dihydroartemisinin, dihydroartemisinin plus<br />
piperaquine, fansidar, mefloquine, piperaquine, primaquine, pyrimethamine, pyronaridine, sulfadoxine, unclassified drug;<br />
anemia, Artemisia annua, combination chemotherapy, cost effectiveness analysis, diagnostic test, disease transmission, drug<br />
cost, drug efficacy, drug half life, endemic disease, fever, gametocyte, gastrointestinal symptom, glucose 6 phosphate<br />
dehydrogenase deficiency, hemolysis, human, malaria, malaria control, malaria falciparum, mixed<br />
infection, morbidity, mortality, parasite control, Plasmodium falciparum, Plasmodium vivax, Plasmodium vivax malaria, priority<br />
journal, relapse, review, zoology; Antimalarials, Artemisinins, Chloroquine, Drug Resistance, Drug Therapy,<br />
Combination, Humans, Malaria, Falciparum, Malaria, Vivax, Plasmodium falciparum, Plasmodium vivax<br />
110
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : Early parasitological diagnosis and treatment with artemisinin-based combination therapies (ACTs) are key components <strong>of</strong><br />
worldwide malaria elimination programmes. In general, use <strong>of</strong> ACTs has been limited to patients with falciparum malaria<br />
whereas blood-stage infections with Plasmodium vivax are mostly still treated with chloroquine. We review the evidence for<br />
the relative benefits and disadvantages <strong>of</strong> the existing separate treatment approach versus a unified ACT-based strategy for<br />
treating Plasmodium falciparum and P vivax infections in regions where both species are endemic (co-endemic). The separate<br />
treatment scenario is justifiable if P vivax remains sensitive to chloroquine and diagnostic tests reliably distinguish P vivax from<br />
P falciparum. However, with the high number <strong>of</strong> misdiagnoses in routine practice and the rise and spread <strong>of</strong> chloroquineresistant<br />
P vivax, there might be a compelling rationale for a unified ACT-based strategy for vivax and falciparum malaria in all<br />
co-endemic regions. Analyses <strong>of</strong> the cost-effectiveness <strong>of</strong> ACTs for both Plasmodium species are needed to assess the role <strong>of</strong><br />
these drugs in the control and elimination <strong>of</strong> vivax malaria. © <strong>2010</strong> Elsevier Ltd. All rights reserved.<br />
No.304<br />
Author (s) : Duangchinda, T., Dejnirattisai, W., Vasanawathana, S., Limpitikul, W., Tangthawornchaikul, N., Malasit, P., Mongkolsapaya, J.,<br />
Screaton, G.<br />
Title : Immunodominant T-cell responses to dengue virus NS3 are associated with DHF<br />
Source : Proceedings <strong>of</strong> the National Academy <strong>of</strong> Sciences <strong>of</strong> the United States <strong>of</strong> America, Volume 107, Issue 39, 28 September <strong>2010</strong>,<br />
Pages 16922-16927<br />
Document Type : Article<br />
Keywords : Cytokine; Flavivirus; Immunopathogenesis<br />
<strong>Abstract</strong> : Dengue infections are increasing at an alarming rate in many tropical and subtropical countries, where epidemics can put<br />
health care systems under extreme pressure. The more severe infections lead to dengue hemorrhagic fever (DHF), which can<br />
be life threatening. A variety <strong>of</strong> viral and host factors have been associated with the severity <strong>of</strong>dengue infections. Because<br />
secondarydengue infectionismore commonly associated withDHF than primary infections, the acquired immune response to<br />
dengue, both B cells and T cells have been implicated. In this study, we set out to study T-cell responses across the entire<br />
dengue virus proteome and to see whether these were related to disease severity in a cohort <strong>of</strong> dengue-infected children<br />
from Thailand. Robust responses were observed in most infected individuals against most viral proteins. Responses to NS3<br />
were the most frequent, and there was a very strong association between the magnitude <strong>of</strong> the response and disease<br />
severity. Furthermore, in DHF, cytokine-high CD107a-negative cells predominated.<br />
No.305<br />
Author (s) : Duangmano, S., Dakeng, S., Jiratchariyakul, W., Suksamrarn, A., Smith, D.R., Patmasiriwat, P.<br />
Title : Antiproliferative effects <strong>of</strong> cucurbitacin B in breast cancer cells: Down-regulation <strong>of</strong> the c-Myc/hTERT/telomerase pathway and<br />
obstruction <strong>of</strong> the cell cycle<br />
Source : <strong>International</strong> Journal <strong>of</strong> Molecular Sciences, Volume 11, Issue 12, December <strong>2010</strong>, Pages 5323-5338<br />
Document Type : Article<br />
Keywords : Breast cancer; c-Myc; Cucurbitacin B; Estrogen receptor; hTERT; Telomerase<br />
<strong>Abstract</strong> : Naturally occurring cucurbitacins have been shown to have anticancer, antimicrobial and anti-inflammatory activities. In this<br />
study, we determined the effects <strong>of</strong> cucurbitacin B extracted from the Thai herb Trichosanthes cucumerina L. on telomerase<br />
regulation in three human breast cancer cell lines (T47D, SKBR-3, and MCF-7) and a mammary epithelium cell line (HBL-100).<br />
Cell viability after treatment with cucurbitacin B, which is an active ingredient <strong>of</strong> this herb, was assessed. Telomeric Repeat<br />
Amplification Protocol (TRAP) assays and RT-PCR (qualitative and realtime) were performed to investigate activity <strong>of</strong><br />
telomerase as well as expression <strong>of</strong> human telomerase reverse transcriptase (hTERT) and c-Myc. The c-Myc protein level was<br />
also determined in SKBR-3 and HBL-100 cells. Our results show that the cucurbitacin B inhibits growth and telomerase activity<br />
in the three breast cancer cell lines and exerts an obvious inhibitory effect in the estrogen receptor (ER)-negative breast<br />
cancer SKBR-3 cells. The expression <strong>of</strong> hTERT and c-Myc were also inhibited by cucurbitacin B, In addition, a clear reduction<br />
<strong>of</strong> c-Myc protein was observed after treatment in SKBR-3 cells even with a concentration <strong>of</strong> cucurbitacin B that was ten-times<br />
lower compared to the concentration used for HBL-100. Our findings imply that cucurbitacin B exerts an anticancer effect by<br />
inhibiting telomerase via down regulating both the hTERT and c-Myc expression in breast cancer cells. © <strong>2010</strong> by the authors.<br />
No.306<br />
Author (s) : Duangpan, S., Jitrapakdee, S., Adina-Zada, A., Byrne, L., Zeczycki, T.N., St. Maurice, M., Cleland, W.W., Wallace, J.C., Attwood,<br />
P.V.<br />
111
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Probing the catalytic roles <strong>of</strong> arg548 and gln552 in the carboxyl transferase domain <strong>of</strong> the rhizobium etli pyruvate carboxylase<br />
by site-directed mutagenesis<br />
Source : Biochemistry, Volume 49, Issue 15, 20 April <strong>2010</strong>, Pages 3296-3304<br />
Document Type : Article<br />
Keywords : Active site, Biotin carboxylase, Carbamoyl phosphates, Carboxyl transferase, Catalytic role, Catalyze reaction, Partial<br />
reactions, Pyruvate carboxylase, Pyruvates, Quaternary structure, Rhizobium etli, Site directed mutagenesis, Wild-type<br />
enzymes; Amino acids, Carboxylation, Catalysis, Coenzymes, Enzymes, Mutagenesis, Phosphorylation, Protons; Polypeptides;<br />
mutant protein, pyruvate carboxylase; article, catalysis, enzyme active site, enzyme activity, enzyme analysis, enzyme<br />
structure, nonhuman, priority journal, protein domain, Rhizobium etli, site directed mutagenesis; Arginine, Bacterial<br />
Proteins, Binding Sites, Biotin, Catalysis, Catalytic Domain, Glutamine, Kinetics, Magnetic Resonance Spectroscopy, Models,<br />
Molecular, Multiprotein Complexes, Mutagenesis, Site-Directed, Protein Conformation, Protein Structure, Quaternary, Pyruvate<br />
Carboxylase, Rhizobium etli; Rhizobium etli<br />
<strong>Abstract</strong> : The roles <strong>of</strong> Arg548 and Gln552 residues in the active site <strong>of</strong> the carboxyl transferase domain <strong>of</strong> Rhizobium etli pyruvate<br />
carboxylase were investigated using site-directed mutagenesis. Mutation <strong>of</strong> Arg548 to alanine or glutamine resulted in the<br />
destabilization <strong>of</strong> the quaternary structure <strong>of</strong> the enzyme, suggesting that this residue has a structural role. Mutations R548K,<br />
Q552N, and Q552A resulted in a loss <strong>of</strong> the ability to catalyze pyruvate carboxylation, biotin-dependent decarboxylation <strong>of</strong><br />
oxaloacetate, and the exchange <strong>of</strong> protons between pyruvate and water. These mutants retained the ability to catalyze<br />
reactions that occur at the active site <strong>of</strong> the biotin carboxylase domain, i.e., bicarbonate-dependent ATP cleavage and ADP<br />
phosphorylation by carbamoyl phosphate. The effects <strong>of</strong> oxamate on the catalysis in the biotin carboxylase domain by the<br />
R548K and Q552N mutants were similar to those on the catalysis <strong>of</strong> reactions by the wild-type enzyme. However, the<br />
presence <strong>of</strong> oxamate had no effect on the reactions catalyzed by the Q552A mutant. We propose that Arg548 and Gln552<br />
facilitate the binding <strong>of</strong> pyruvate and the subsequent transfer <strong>of</strong> protons between pyruvate and biotin in the partial reaction<br />
catalyzed in the active site <strong>of</strong> the carboxyl transferase domain <strong>of</strong> Rhizobium etli pyruvate carboxylase. © <strong>2010</strong> American<br />
Chemical Society.<br />
No.307<br />
Author (s) : Duangtep, Y., Narksawat, K., Chongsuwat, R., Rojanavipart, P.<br />
Title : Association between an unhealthy lifestyle and other factors with hypertension among hill tribe populations <strong>of</strong> Mae Fah<br />
Luang district, Chiang Rai Province, Thailand<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 3, May <strong>2010</strong>, Pages 726-734<br />
Document Type : Article<br />
Keywords : Hill tribe population; Hypertension; Thailand; Unhealthy lifestyle<br />
<strong>Abstract</strong> : An unhealthy lifestyle may lead to hypertension which can cause strokes and cardiovascular disease. The aim <strong>of</strong> this study<br />
was to identify the specific unhealthy lifestyle practices which could cause hypertension among hill tribe populations in Mae<br />
Fah Luang District <strong>of</strong> Chiang Rai Province, Thailand. In 2006, 196 patients with hypertension were selected from 2 district<br />
hospitals and 13 health centers as cases, and 196 normotensive subjects from a local neighborhood were chosen as controls.<br />
Trained health personnel collected data by interviewing subjects from both groups regarding unhealthy lifestyles and other<br />
factors. All participants had a physical examination at the time <strong>of</strong> interview. The results from multiple logistic regression<br />
analysis show the factors associated with hypertension among the hill tribe people studied were smoking (OR 2.48; 95%CI<br />
1.43-4.30, p = 0.001), no or irregular exercise (OR 1.84; 95%CI 1.16-2.99, p = 0.005), being overweight (OR 2.96; 95%CI 1.69-<br />
5.18, p < 0.002), having obesity (OR 2.65, 95%CI 1.04-6.73, p < 0.018) and having a high frequency intake <strong>of</strong> fatty foods (OR<br />
1.98, 95%CI 1.23-3.18, p < 0.013). The findings suggest the need for significant lifestyle changes in regards to smoking, eating<br />
habits and leisure time exercise programs. The adoption <strong>of</strong> such lifestyle changes would result in a reduced chance <strong>of</strong> being<br />
hypertensive, which could later reduce cardiovascular morbidity and mortality.<br />
No.308<br />
Author (s) : Duckworth, J.W., Boonratana, R., Robichaud, W.G., Timmins, R.J.<br />
Title : A review <strong>of</strong> François' leaf monkey Trachypithecus francoisi (sensu lato) in Lao PDR<br />
Source : Primate Conservation, Volume 25, Issue 1, December <strong>2010</strong>, Pages 61-79<br />
Document Type : Review<br />
Keywords : Conservation status; distribution; habitat; Trachypithecus ( francoisi) ebenus; Trachypithecus ( francoisi)<br />
hatinhensis; Trachypithecus ( francoisi) laotum<br />
112
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : A monkey population allied to François' leaf monkey Trachypithecus francoisi discovered in 1920 constitutes the taxon<br />
laotum, endemic to Lao PDR. The next seven decades provided very little additional information about it. Wildlife surveys in<br />
the 1990s found a large population <strong>of</strong> T. (f.) laotum in Phou Hinpoun (=Khammouan Limestone) National Protected Area<br />
(NPA) and north into southern Nam Kading NPA, and a large population in and around Hin Namno NPA <strong>of</strong> T. (f.) ebenus,<br />
known outside Lao PDR only from adjoining Vietnam. Detailed here are confirmed records <strong>of</strong> smaller and more localized<br />
populations <strong>of</strong> leaf monkeys <strong>of</strong> the T. francoisi group (sensu Groves 2001, 2005, p. 175) from two other areas (Nakai-Nam<br />
Theun NPA and a region outside the protected area system, Muang (= District <strong>of</strong>) Vilabouli, in Savannakhet province), highly<br />
plausible reports from one more site, and records <strong>of</strong> T. (f.) ebenus from southern Phou Hinpoun NPA. Animals from Nakai-<br />
Nam Theun NPA and Muang Vilabouli differ in pelage from reported forms, and further information is required to resolve their<br />
taxonomy in relation to the named forms hatinhensis and ebenus. All Lao records <strong>of</strong> the Trachypithecus francoisi group leaf<br />
monkeys are within the latitudinal band <strong>of</strong> 16°58′N (probably 16°49′N) to 18°17′N, but reports from local people suggest the<br />
possibility <strong>of</strong> occurrence north <strong>of</strong> this latitude, and perhaps (parallel with the complex's distribution in Vietnam) north to the<br />
Chinese border. Populations in large karst landscapes remain healthy but cannot be assumed to remain so, and those in<br />
smaller karst and non-calcareous ranges are highly vulnerable to hunting-induced local extinction. Many uncertainties remain<br />
concerning the species-complex in Lao PDR: its overall distribution, the number <strong>of</strong> forms present, their distribution, and their<br />
taxonomy. Undescribed forms may yet be found, most likely to the north <strong>of</strong> the known range, where threats are much<br />
higher, adding to the urgency for surveys in this region.<br />
No.309<br />
Author (s) : Dujardin, J.-P.A., Kaba, D., Henry, A.B.<br />
Title : The exchangeability <strong>of</strong> shape<br />
Source : BMC Research Notes, Volume 3, <strong>2010</strong>, Article number 266<br />
Document Type : Article<br />
Keywords : –<br />
<strong>Abstract</strong> : Background. Landmark based geometric morphometrics (GM) allows the quantitative comparison <strong>of</strong> organismal shapes. When<br />
applied to systematics, it is able to score shape changes which <strong>of</strong>ten are undetectable by traditional morphological studies<br />
and even by classical morphometric approaches. It has thus become a fast and low cost candidate to identify cryptic species.<br />
Due to inherent mathematical properties, shape variables derived from one set <strong>of</strong> coordinates cannot be compared with<br />
shape variables derived from another set. Raw coordinates which produce these shape variables could be used for data<br />
exchange, however they contain measurement error. The latter may represent a significant obstacle when the objective is to<br />
distinguish very similar species. Results. We show here that a single user derived dataset produces much less classification<br />
error than a multiple one. The question then becomes how to circumvent the lack <strong>of</strong> exchangeability <strong>of</strong> shape variables<br />
while preserving a single user dataset. A solution to this question could lead to the creation <strong>of</strong> a relatively fast and<br />
inexpensive systematic tool adapted for the recognition <strong>of</strong> cryptic species. Conclusions. To preserve both exchangeability <strong>of</strong><br />
shape and a single user derived dataset, our suggestion is to create a free access bank <strong>of</strong> reference images from which one<br />
can produce raw coordinates and use them for comparison with external specimens. Thus, we propose an alternative<br />
geometric descriptive system that separates 2-D data gathering and analyzes. © <strong>2010</strong> Dujardin et al; licensee BioMed Central<br />
Ltd.<br />
No.310<br />
Author (s) : Dunham, K.A., Siriphorn, A., Chompoopong, S., Floyd, C.L.<br />
Title : Characterization <strong>of</strong> a graded cervical hemicontusion spinal cord injury model in adult male rats<br />
Source : Journal <strong>of</strong> Neurotrauma, Volume 27, Issue 11, 1 November <strong>2010</strong>, Pages 2091-2106<br />
Document Type : Article<br />
Keywords : forepaw function; gliosis; gray-matter sparing; white-matter sparing<br />
<strong>Abstract</strong> : Most experimental models <strong>of</strong> spinal cord injury (SCI) in rodents induce damage in the thoracic cord and subsequently<br />
examine hindlimb function as an indicator <strong>of</strong> recovery. In these models, functional recovery is most attributable to whitematter<br />
preservation and is less influenced by grey-matter sparing. In contrast, most clinical cases <strong>of</strong> SCI occur at the lower<br />
cervical levels, a region in which both grey-matter and white-matter sparing contribute to functional motor recovery. Thus<br />
experimental cervical SCI models are beginning to be developed and used to assess protective and pharmacological<br />
interventions following SCI. The objective <strong>of</strong> this study was to characterize a model <strong>of</strong> graded cervical hemicontusion SCI with<br />
regard to several histological and behavioral outcome measures, including novel forelimb behavioral tasks. Using a<br />
113
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
commercially available rodent spinal cord impactor, adult male rats received hemicontusion SCI at vertebral level C5 at 100,<br />
200, or 300kdyn force, to produce mild, moderate, or severe injury severities. Tests <strong>of</strong> skilled and unskilled forelimb and<br />
locomotor function were employed to assess functional recovery, and spinal cord tissue was collected to assess lesion<br />
severity. Deficits in skilled and unskilled forelimb function and locomotion relating to injury severity were observed, as well as<br />
decreases in neuronal numbers, white-matter area, and white-matter gliosis. Significant correlations were observed between<br />
behavioral and histological data. Taken together, these data suggest that the forelimb functional and locomotor assessments<br />
employed here are sensitive enough to measure functional changes, and that this hemicontusion model can be used to<br />
evaluate potential protective and regenerative therapeutic strategies. © Copyright <strong>2010</strong>, Mary Ann Liebert, Inc.<br />
No.311<br />
Author (s) : Dusotoit-Coucaud, A., Kongsawadworakul, P., Maurousset, L., Viboonjun, U., Brunel, N., Pujade-Renaud, V., Chrestin, H., Sakr, S.<br />
Title : Ethylene stimulation <strong>of</strong> latex yield depends on the expression <strong>of</strong> a sucrose transporter (HbSUT1B) in rubber tree (Hevea<br />
brasiliensis)<br />
Source : Tree Physiology, Volume 30, Issue 12, December <strong>2010</strong>, Pages 1586-1598<br />
Document Type : Article<br />
Keywords : ethylene; latex yield; laticifers; sugar transporters<br />
<strong>Abstract</strong> : Hevea brasiliensis is an important industrial crop for natural rubber production. Latex biosynthesis occurs in the cytoplasm <strong>of</strong><br />
highly specialized latex cells and requires sucrose as the unique precursor. Ethylene stimulation <strong>of</strong> latex production results in<br />
high sugar flow from the surrounding cells <strong>of</strong> inner bark towards the latex cells. The aim <strong>of</strong> this work was to understand the<br />
role <strong>of</strong> seven sucrose transporters (HbSUTs) and one hexose transporter (HbHXT1) in this process. Two Hevea clones were<br />
used: PB217 and PB260, respectively described as high and low yielding clones. The expression pattern <strong>of</strong> these sugar<br />
transporters (HbSUTs and HbHXT1) was monitored under different physiological conditions and found to be maximal in latex<br />
cells. HbSUT1, one <strong>of</strong> the most abundant is<strong>of</strong>orms, displayed the greatest response to ethylene treatment. In clone PB217,<br />
ethylene treatment led to a higher accumulation <strong>of</strong> HbSUT1B in latex cells than in the inner bark tissues. Conversely, stronger<br />
expression <strong>of</strong> HbSUT1B was observed in inner bark tissues than in latex cells <strong>of</strong> PB260. A positive correlation with HbSUT1B<br />
transcript accumulation and increased latex production was further supported by its lower expression in latex cells <strong>of</strong> the<br />
virgin clone PB217. © The Author <strong>2010</strong>. Published by Oxford <strong>University</strong> Press. All rights reserved.<br />
No.312<br />
Author (s) : Dusotoit-Coucaud, A., Porcheron, B., Brunel, N., Kongsawadworakul, P., Franchel, J., Viboonjun, U., Chrestin, H., Lemoine, R.,<br />
Sakr, S.<br />
Title : Cloning and characterization <strong>of</strong> a new polyol transporter (HbPLT2) in Hevea brasiliensis<br />
Source : Plant and Cell Physiology, Volume 51, Issue 11, November <strong>2010</strong>, Pages 1878-1888<br />
Document Type : Article<br />
Keywords : Hevea brasiliensis; Latex production; Polyol transporter; Quebrachitol<br />
<strong>Abstract</strong> : Quebrachitol is a cyclic polyol and, along with sucrose, is one <strong>of</strong> the main sugars in Hevea latex. However, in contrast to<br />
sucrose, the mechanism and regulation <strong>of</strong> quebrachitol absorption is still unknown. Screening a latex-derived cDNA library<br />
using polyol transporter-specific probes, two full-length cDNAs were isolated, and named HbPLT1 and HbPLT2 (for Hevea<br />
brasiliensis polyol transporter 1 and 2, respectively). Their respective sequences exhibited close similarity with the previously<br />
cloned acyclic sugar polyol transporters, and shared the main features <strong>of</strong> the major facilitative superfamily. The functional<br />
activity <strong>of</strong> one <strong>of</strong> the cDNAs was determined by using an HbPLT2-complemented yeast strain. These strains displayed a<br />
marginal absorption <strong>of</strong> cyclic (inositol) and acyclic (mannitol and sorbitol) polyol but no absorption <strong>of</strong> sucrose, hexose and<br />
glycerol. Active absorption for xylitol was detected, and was competitively inhibited by quebrachitol. HbPLT1 and HbPLT2<br />
expression patterns varied in response to different stimuli. Bark treatment with ethylene resulted in an early and significant<br />
up-regulation <strong>of</strong> HbPLT2 transcripts in laticifers as well as in inner bark cells, when compared with HbPLT1. Other treatments,<br />
especially mechanical wounding, strongly induced HbPLT2 transcripts. These data were consistent with the presence <strong>of</strong><br />
ethylene and a wound-responsive regulatory cis-element on the sequence <strong>of</strong> the HbPLT2 promoter. All these findings<br />
together with those recently obtained for sucrose transporters and aquaporins are discussed in relation to the different roles<br />
for quebrachitol in Hevea brasiliensis. © <strong>2010</strong> The Author.<br />
No.313<br />
Author (s) : Dussadee, K., Taka, O., Thedsawad, A., Wanachiwanawin, W.<br />
114
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Incidence and risk factors <strong>of</strong> relapses in idiopathic autoimmune hemolytic anemia.<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand = Chotmaihet thangphaet, Volume 93 Suppl 1, January <strong>2010</strong>, Pages S165-170<br />
Document Type : Article<br />
Keywords : autoantibody, glucocorticoid, prednisolone; adolescent, adult, aged, article, autoimmune hemolytic anemia, blood, cohort<br />
analysis, Coombs test, female, follow up, human, immunology, incidence, male, medical record, middle aged, prospective<br />
study, recurrent disease, retrospective study, risk factor, Thailand; Adolescent, Adult, Aged, Anemia, Hemolytic,<br />
Autoimmune, Autoantibodies, Cohort Studies, Coombs' Test, Female, Follow-Up<br />
Studies, Glucocorticoids, Humans, Incidence, Male, Medical Records, Middle Aged, Prednisolone, Prospective<br />
Studies, Recurrence, Retrospective Studies, Risk Factors, Thailand, Young Adult<br />
<strong>Abstract</strong> : BACKGROUND: Patients with idiopathic autoimmune hemolytic anemia (AIHA) <strong>of</strong> warm antibody type usually respond to<br />
corticosteroid therapy. However a proportion <strong>of</strong> patients will have disease relapse after steroid-induced remission. OBJECTIVE:<br />
To assess the incidence and the possible risk factors <strong>of</strong> the relapse in a cohort <strong>of</strong> patients with idiopathic AIHA. MATERIAL<br />
AND METHOD: We conducted a retrospective and prospective study <strong>of</strong> 34 idiopathic AIHA patients regularly followed at the<br />
Division <strong>of</strong> Hematology during January 1973 to December 2006. The medical records were reviewed for active hemolytic<br />
events and relapses, episodes <strong>of</strong> infections, pattern <strong>of</strong> corticosteroids administration and tapering. Types and subtypes <strong>of</strong><br />
autoantibodies were studied by column agglutination test (the "gel test"). RESULTS: One patient with cold agglutinin disease<br />
was excluded, leaving a total <strong>of</strong> 33 patients (24 with warm type, 9 with mixed warm and cold type AIHA) in the study. The<br />
incidence <strong>of</strong> relapse was 1.157 episodes/person/year. The mean duration <strong>of</strong> relapse after remission was 23 months. Episodes<br />
<strong>of</strong> recurrent hemolysis were more frequent when corticosteroid administration was tapered from high to low dose (10 mg/day<br />
<strong>of</strong> prednisolone) within two months compared with a longer than two-month tapering (38 vs. 11 episodes; p < 0.01). In<br />
addition patients receiving continuing low dose <strong>of</strong> corticosteroids (< or = 10 mg/day <strong>of</strong> prednisolone) for > 6 months had<br />
lower incidence <strong>of</strong> relapse and longer duration <strong>of</strong> remission than those with discontinuing the medication within 6 months<br />
(0.443 vs. 1.911 episodes/person/year, p < 0.01; 37.4 vs. 10.6 months, p < 0.01). Episodes <strong>of</strong> recurrent hemolysis were more<br />
frequently observed in patients with events <strong>of</strong> infection than those without infections (mean 7.69 vs. 2.81, p = 0.032). Types<br />
and subtypes <strong>of</strong> autoantibodies did not seem to influence relapse in AIHA. CONCLUSION: Short duration <strong>of</strong> maintenance and<br />
rapid tapering <strong>of</strong> corticosteroids and infections are possible risk factors <strong>of</strong> relapses/recurrent hemolysis in idiopathic AIHA.<br />
No.314<br />
Author (s) : Eamsobhana, P., Lim, P.E., Solano, G., Zhang, H., Gan, X., Yong, H.S.<br />
Title : Molecular differentiation <strong>of</strong> Angiostrongylus taxa (Nematoda: Angiostrongylidae) by cytochrome c oxidase subunit I (COI) gene<br />
sequences<br />
Source : Acta Tropica, Volume 116, Issue 2, November <strong>2010</strong>, Pages 152-156<br />
Document Type : Article<br />
Keywords : Angiostrongylus cantonensis; Angiostrongylus costaricensis; Angiostrongylus malaysiensis; Angiostrongylus<br />
vasorum; Molecular phylogeny; Parastrongylus; Phylogeography<br />
<strong>Abstract</strong> : Nematodes <strong>of</strong> the genus Angiostrongylus are parasites <strong>of</strong> rodents and carnivores. They reside in the pulmonary or mesenteric<br />
arteries <strong>of</strong> their hosts. Two species are pathogenic in humans - Angiostrongylus cantonensis causes eosinophilic meningitis or<br />
meningoencephalitis, and Angiostrongylus costaricensis produces abdominal angiostrongyliasis. In addition Angiostrongylus<br />
malaysiensis may have the potential <strong>of</strong> being pathogenic in humans. The mitochondrial gene cytochrome c oxidase subunit I<br />
(COI) <strong>of</strong> these Angiostrongylus species and three geographical isolates (China, Hawaii and Thailand) <strong>of</strong> A. cantonensis were<br />
studied by polymerase chain reaction amplification and DNA sequencing. COI sequences <strong>of</strong> A. cantonensis, A. costaricensis<br />
and Angiostrongylus vasorum in the GenBank were included for comparison. Phylogenetic analysis by maximum-likelihood<br />
(ML), maximum-parsimony (MP), neighbour-joining (NJ) and Bayesian inference (BI) produced similar tree topology except<br />
variation in the bootstrap support values. There were two major clades - (1) A. cantonensis and A. malaysiensis, and (2) A.<br />
costaricensis and A. vasorum. The three geographical isolates <strong>of</strong> A. cantonensis formed a clade with low to high bootstrap<br />
values, and consisted <strong>of</strong> two subclades: (a) China and Hawaii isolates, and (b) monophyletic Thailand isolate. The individuals<br />
<strong>of</strong> each isolate formed a distinct cluster. In the second major clade, the Europe isolates <strong>of</strong> A. vasorum were distinctly<br />
different from the Brazil isolates. For A. costaricensis, the Costa Rica isolate was distinct from the Brazil isolate with an<br />
uncorrected (p) distance <strong>of</strong> 11.39%, indicating the possible occurrence <strong>of</strong> cryptic species. The present results indicate that COI<br />
sequences might be a useful marker for differentiating geographical isolates <strong>of</strong> A. cantonensis and in uncovering cryptic<br />
species. Efforts are being made to carry out an extensive collaborative study to cover a wide range <strong>of</strong> Angiostrongylus species<br />
and geographical isolates. © <strong>2010</strong> Elsevier B.V.<br />
115
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.315<br />
Author (s) : Eamsobhana, P., Lim, P.E., Zhang, H., Gan, X., Yong, H.S.<br />
Title : Molecular differentiation and phylogenetic relationships <strong>of</strong> three Angiostrongylus species and Angiostrongylus cantonensis<br />
geographical isolates based on a 66-kDa protein gene <strong>of</strong> A. cantonensis (Nematoda: Angiostrongylidae)<br />
Source : Experimental Parasitology, Volume 126, Issue 4, December <strong>2010</strong>, Pages 564-569<br />
Document Type : Article<br />
Keywords : Angiostrongylus cantonensis; Angiostrongylus costaricensis; Angiostrongylus malaysiensis; DNA sequences; Molecular<br />
differentiation; Phylogenetic relationships<br />
<strong>Abstract</strong> : The phylogenetic relationships and molecular differentiation <strong>of</strong> three species <strong>of</strong> angiostrongylid nematodes (Angiostrongylus<br />
cantonensis, Angiostrongylus costaricensis and Angiostrongylus malaysiensis) were studied using the AC primers for a 66-kDa<br />
protein gene <strong>of</strong> A. cantonensis. The AC primers successfully amplified the genomic DNA <strong>of</strong> these angiostrongylid nematodes.<br />
No amplification was detected for the DNA <strong>of</strong> Ascaris lumbricoides, Ascaris suum, Anisakis simplex, Gnathostoma spinigerum,<br />
Toxocara canis, and Trichinella spiralis. The maximum-parsimony (MP) consensus tree and the maximum-likelihood (ML) tree<br />
both showed that the Angiostrongylus taxa could be divided into two major clades - Clade 1 (A. costaricensis) and Clade 2 (A.<br />
cantonensis and A. malaysiensis) with a full support bootstrap value. A. costaricensis is the most distant taxon. A. cantonensis<br />
is a sister group to A. malaysiensis; these two taxa (species) are clearly separated. There is no clear distinction between the A.<br />
cantonensis samples from four different geographical localities (Thailand, China, Japan and Hawaii); only some <strong>of</strong> the samples<br />
are grouped ranging from no support or low support to moderate support <strong>of</strong> bootstrap values. The published nucleotide<br />
sequences <strong>of</strong> A. cantonensis adult-specific native 66. kDa protein mRNA, clone L5-400 from Taiwan (U17585) appear to be<br />
very distant from the A. cantonensis samples from Thailand, China, Japan and Hawaii, with the uncorrected p-distance values<br />
ranging from 26.87% to 29.92%. © Elsevier Inc.<br />
No.316<br />
Author (s) : Eamsobhana, P., Yoolek, A., Yong, H.-S.<br />
Title : Effect <strong>of</strong> Thai 'koi-hoi' food flavoring on the viability and infectivity <strong>of</strong> the third-stage larvae <strong>of</strong> Angiostrongylus cantonensis<br />
(Nematoda: Angiostrongylidae)<br />
Source : Acta Tropica, Volume 113, Issue 3, March <strong>2010</strong>, Pages 245-247<br />
Document Type : Article<br />
Keywords : Angiostrongylus cantonensis; Eosinophilic meningitis; Food-borne parasitic disease; Snail; Thai food; Worm burden<br />
<strong>Abstract</strong> : The effect <strong>of</strong> the food flavoring <strong>of</strong> 'koi-hoi', a popular Thai snail dish, on the viability and infectivity <strong>of</strong> Angiostrongylus<br />
(=Parastrongylus) cantonensis third-stage larvae was assessed in a mouse model. Groups <strong>of</strong> 50 each <strong>of</strong> actively moving, nonmotile<br />
coiled, and extended larvae were obtained from experimentally infected snail meat, after one-hour exposure to<br />
standard 'koi-hoi' flavoring. These larvae and groups <strong>of</strong> 50 unexposed moving larvae (control) were individually fed to each<br />
group <strong>of</strong> three experimental BALB/c mice. The effect on Angiostrongylus worm burden was measured after 3 weeks <strong>of</strong><br />
infection. Infectivity <strong>of</strong> the motile larvae after exposure to 'koi-hoi' food flavoring was 38±5.29%. This was highly significantly<br />
lower than the infectivity (62±7.21%) <strong>of</strong> the control (unexposed) third-stage larvae (χ2=17.28, P<0.001). In the non-motile<br />
larvae resulting from exposure to the food flavoring, no adult worm was recovered from the extended larvae, indicating that<br />
they were no longer alive and unable to cause infection. A small proportion (3.33±2.31%) <strong>of</strong> the coiled larvae developed into<br />
young adult worms, indicating that mobility alone is not a definitive indicator <strong>of</strong> viability. The present study confirms that the<br />
food flavoring components <strong>of</strong> 'koi-hoi' dish adversely affect the viability and infectivity <strong>of</strong> A. cantonensis larvae. Exposure <strong>of</strong><br />
the third-stage larvae to 'koi-hoi' food flavoring resulted in decreased viability and eventually death. Prolonged treatment with<br />
food flavoring to inactivate/immobilize and then kill the infective, third-stage larvae <strong>of</strong> A. cantonensis in snail meat prior to<br />
consumption may be one <strong>of</strong> the possible economical means <strong>of</strong> reducing human infection. © 2009 Elsevier B.V.<br />
No.317<br />
Author (s) : Engrav, L.H., Heimbach, D.M., Rivara, F.P., Moore, M.L., Wang, J., Carrougher, G.J., Costa, B., Numhom, S., Calderon, J., Gibran,<br />
N.S.<br />
Title : 12-Year within-wound study <strong>of</strong> the effectiveness <strong>of</strong> custom pressure garment therapy<br />
Source : Burns, Volume 36, Issue 7, November <strong>2010</strong>, Pages 975-983<br />
Document Type : Article<br />
Keywords : Burns; Colorimeter; Durometer; Fibroproliferation; Hypertrophic scar; I-Scan; Pressure garment therapy; Thermal<br />
injury; Ultrasound<br />
116
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : Pressure garment therapy is standard <strong>of</strong> care for prevention and treatment <strong>of</strong> hypertrophic scarring after burn injury.<br />
Nevertheless there is little objective data that confirms effectiveness. The purpose <strong>of</strong> this study was to determine the<br />
effectiveness <strong>of</strong> pressure garment therapy with objective data obtained with a randomized within-wound comparison. We<br />
enrolled consecutive patients with forearm injuries over a 12-year period. The subjects wore custom garments with normal<br />
and low compression randomized to either the proximal or distal zones. Hardness, color and thickness <strong>of</strong> wounds were<br />
objectively measured using appropriate devices; clinical appearance was measured by a panel masked to the identity <strong>of</strong> the<br />
pressure treated area. Wounds treated with normal compression were significantly s<strong>of</strong>ter, thinner, and had improved clinical<br />
appearance. There was no interaction <strong>of</strong> any effect with patient ethnicity. However, these findings were clinically evident only<br />
with moderate to severe scarring. We conclude that pressure garment therapy is effective, but that the clinical benefit is<br />
restricted to those patients with moderate or severe scarring. © <strong>2010</strong> Elsevier Ltd and ISBI. All rights reserved.<br />
No.318<br />
Author (s) : Ervithayasuporn, V., Abe, J., Wang, X., Matsushima, T., Murata, H., Kawakami, Y.<br />
Title : Synthesis, characterization, and OLED application <strong>of</strong> oligo(p-phenylene ethynylene)s with polyhedral oligomeric<br />
silsesquioxanes (POSS) as pendant groups<br />
Source : Tetrahedron, Volume 66, Issue 48, 27 November <strong>2010</strong>, Pages 9348-9355<br />
Document Type : Article<br />
Keywords : Light-emitting diode; Oligomer; p-Phenylene ethynylene; Photoluminescence; Silsesquioxane<br />
<strong>Abstract</strong> : Two new classes <strong>of</strong> mono- and oligo(p-phenylene ethynylene)s grafted with polyhedral oligomeric silsesquioxanes (POSS)<br />
were synthesized via 'click' chemistry and palladium-catalyzed Sonogashira cross-coupling. These materials with cubic<br />
silsesquioxanes are very robust with excellent thermal stability in air (T5%loss>330 °C) and exhibited Tg>80 °C. All the<br />
compounds showed high photoluminescence with a range <strong>of</strong> blue emission and quantum yield up to 80% in the solution.<br />
Extended π conjugation molecules <strong>of</strong> oligo-pPEs POSS maintain relatively high PL quantum efficiencies in the solid state,<br />
compared to mono-pPEs POSS. A preliminary report is made <strong>of</strong> some <strong>of</strong> the materials as multilayer OLED components with<br />
active dopants PVK and PBD. © <strong>2010</strong> Elsevier Ltd. All rights reserved.<br />
No.319<br />
Author (s) : Eu-Ahsunthornwattana, J., Trachoo, O., Dejsuphong, D., Tunteeratum, A., Srichan, K., Sura, T.<br />
Title : Noonan syndrome, metabolic syndrome and stroke-in-the-young: Coincidence, causal or contribution?<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 9, September <strong>2010</strong>, Pages 1084-1087<br />
Document Type : Article<br />
Keywords : Cerebral infarction; Diabetes mellitus; Metabolic syndrome; Multifactorial causality; Noonan syndrome<br />
<strong>Abstract</strong> : A 34-year-old Thai woman developed acute left hemiparesis with dysarthria from subcortical infarction <strong>of</strong> the right MCA<br />
territory eighteen months after being diagnosed with Noonan syndrome, diabetes mellitus, dyslipidaemia, and hypertension.<br />
Further investigations suggested atherosclerosis as a cause. Modifying her risk factors was difficult, artly because <strong>of</strong> limited<br />
adherence. Three years later, she had another attack <strong>of</strong> ischaemic stroke in the same area. Unlike the three previously<br />
reported cases, the causation <strong>of</strong> strokes in this patient appeared to be a more 'complex' interaction between genetic defect<br />
and environment including possible subtle arterial abnormalities, metabolic risk factors, and mental insufficiency.<br />
No.320<br />
Author (s) : Euanorasetr, C., Sornmayura, P.<br />
Title : Surgical management <strong>of</strong> Brunner's gland hamartoma causing upper GI hemorrhage: Report <strong>of</strong> two cases and literature review<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 10, October <strong>2010</strong>, Pages 1232-1237<br />
Document Type : Article<br />
Keywords : Brunner's gland hamartoma; Gastroduodenoscopy; Surgical resection; Upper GI hemorrhage<br />
<strong>Abstract</strong> : Brunner's gland hamartomas are uncommon benign tumor <strong>of</strong> the duodenum. Most lesions are small and asymptomatic.<br />
Occasionally, those lesions may be large and manifest as a rare cause <strong>of</strong> upper GI hemorrhage or duodenal obstruction. The<br />
authors report here two cases <strong>of</strong> Brunner's gland hamartoma presenting with upper GI hemorrhage that were not amenable<br />
to endoscopic polypectomy thus requiring surgical resection. The literature on Brunner's gland hamartoma was reviewed.<br />
No.321<br />
Author (s) : Euanorasetr, J., Nilvongse, A., Tantimavanich, S., Nihira, T., Igarashi, Y., Panbangred, W.<br />
117
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Identification and characterization <strong>of</strong> soil-isolated Streptomyces SJE177 producing actinomycin<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 5, September <strong>2010</strong>, Pages 1177-1187<br />
Keywords : Actinomycin; Antibacterial activity; Co-cultivation method; Streptomyces; Thailand<br />
Document Type : Article<br />
<strong>Abstract</strong> : One hundred seventy-seven actinomycetes strains were isolated from soils collected from fruit orchards in Thailand. All were<br />
tested for antibacterial activity against seven pathogenic bacteria using co-cultivation methods. Forty strains (22.6%) were<br />
active against at least one indicator bacteria. Twenty-seven strains (15.3%) inhibited only gram-positive bacteria, four strains<br />
(2.3%) inhibited only gram-negative bacteria, and nine strains (5.1%) showed activity against both. Strain SJE177 had potent<br />
activity against all tested bacteria, and was selected for further investigation. A crude ethyl acetate extract <strong>of</strong> this strain<br />
retained inhibitory activity as tested by disk-diffusion method. Analysis <strong>of</strong> morphological and biochemical characteristics and<br />
the 16S rRNA gene sequence indicated this strain belonged to the genus Streptomyces. The strain formed a monophyletic<br />
line in a phylogenetic tree <strong>of</strong> 16S rRNA gene sequences with other Streptomyces reference strains. High performance liquid<br />
chromatography (HPLC) analysis showed SJE177 produced actinomycin. Since many isolates showed inhibitory activity against<br />
indicator bacteria, these results suggest Thai soil could be an interesting source to explore for antibacterial substances.<br />
No.322<br />
Author (s) : Farreres, J., Gibert, K., Rodríguez, H., Pluempitiwiriyawej, C.<br />
Title : Inference <strong>of</strong> lexical ontologies. The LeOnI methodology<br />
Source : Artificial Intelligence, Volume 174, Issue 1, January <strong>2010</strong>, Pages 1-19<br />
Document Type : Article<br />
Keywords : Automatic ontology building; Lexico-conceptual ontologies; Logistic regression; WordNet<br />
<strong>Abstract</strong> : In this article we present a method for semi-automatically deriving lexico-conceptual ontologies in other languages, given a<br />
lexico-conceptual ontology for one language and bilingual mapping resources. Our method uses a logistic regression model to<br />
combine mappings proposed by a set <strong>of</strong> classifiers (up to 17 in our implementation). The method is formally described and<br />
evaluated by means <strong>of</strong> two implementations for semi-automatically building Spanish and Thai WordNets using Princeton's<br />
WordNet for English and conventional English-Spanish and English-Thai bilingual dictionaries. © 2009 Elsevier B.V. All rights<br />
reserved.<br />
No.323<br />
Author (s) : Fongsatitkul, P., Elefsiniotis, P., Wareham, D.G.<br />
Title : Effect <strong>of</strong> mixture ratio, solids concentration and hydraulic retention time on the anaerobic digestion <strong>of</strong> the organic fraction <strong>of</strong><br />
municipal solid waste<br />
Source : Waste Management and Research, Volume 28, Issue 9, September <strong>2010</strong>, Pages 811-817<br />
Document Type : Article<br />
Keywords : activated sludge; co-digestion; hydraulic retention time; Municipal solid waste; organic fraction; total solids<br />
<strong>Abstract</strong> : This paper describes how the degradation <strong>of</strong> the organic fraction <strong>of</strong> municipal solid waste (OFMSW) is affected through<br />
codigestion with varying amounts <strong>of</strong> return activated sludge (RAS). Solid waste that had its inorganic fraction selectively<br />
removed was mixed with RAS in ratios <strong>of</strong> 100% OFMSW, 50% OFMSW/50% RAS, and 25% OFMSW/75% RAS. The total solids<br />
(TS) concentration was held at 8% and three anaerobic digester systems treating the mixtures were held (for the first run) at a<br />
total hydraulic retention time (HRT) <strong>of</strong> 28 days. Increasing amounts <strong>of</strong> RAS did not however improve the mixture's<br />
digestability, as indicated by little change and/or a drop in the main performance indices [including percentage volatile solids<br />
(VS) removal and specific gas production]. The optimum ratio in this research therefore appeared to be 100% OFMSW with an<br />
associated 85.1 ± 0.6% VS removal and 0.72 ± 0.01 L total gas g-1 VS. In the second run, the effect <strong>of</strong> increasing percentage<br />
<strong>of</strong> TS (8, 12% and 15%) at a system HRT <strong>of</strong> 28 days was observed to yield no improvement in the main performance indices<br />
(i.e. percentage VS removal and specific gas production). Finally, during the third run, variations in the total system HRT were<br />
investigated at an 8% TS, again using 100% OFMSW. Of the HRTs explored (23, 28 and 33 days), the longest HRT yielded the<br />
best performance overall, particularly in terms <strong>of</strong> specific gas production (0.77 ± 0.01 L total gas g-1 VS). © The Author(s),<br />
<strong>2010</strong>.<br />
No.324<br />
Author (s) : Freathy, R.M., Mook-Kanamori, D.O., Sovio, U., Prokopenko, I., Timpson, N.J., Berry, D.J., Warrington, N.M., Widen, E., Hottenga,<br />
J.J., Kaakinen, M., Lange, L.A., Bradfield, J.P., Kerkh<strong>of</strong>, M., Marsh, J.A., Mägi, R., Chen, C.-M., Lyon, H.N., Kirin, M., Adair, L.S.,<br />
118
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Aulchenko, Y.S., Bennett, A.J., Borja, J.B., Bouatia-Naji, N., Charoen, P., Coin, L.J.M., Cousminer, D.L., De Geus, E.J.C., Deloukas,<br />
P., Elliott, P., Evans, D.M., Froguel, P., Glaser, B., Groves, C.J., Hartikainen, A.-L., Hassanali, N., Hirschhorn, J.N., H<strong>of</strong>man, A., Holly,<br />
J.M.P., Hyppönen, E., Kanoni, S., Knight, B.A., Laitinen, J., Lindgren, C.M., McArdle, W.L., O'Reilly, P.F., Pennell, C.E., Postma, D.S.,<br />
Pouta, A., Ramasamy, A., Rayner, N.W., Ring, S.M., Rivadeneira, F., Shields, B.M., Strachan, D.P., Surakka, I., Taanila, A., Tiesler, C.,<br />
Uitterlinden, A.G., Van Duijn, C.M., Wijga, A.H., Willemsen, G., Zhang, H., Zhao, J., Wilson, J.F., Steegers, E.A.P., Hattersley, A.T.,<br />
Eriksson, J.G., Peltonen, L., Mohlke, K.L., Grant, S.F.A., Hakonarson, H., Koppelman, G.H., Dedoussis, G.V., Heinrich, J., Gillman,<br />
M.W., Palmer, L.J., Frayling, T.M., Boomsma, D.I., Smith, G.D., Power, C., Jaddoe, V.W.V., Jarvelin, M.-R., McCarthy, M.I.<br />
Title : Variants in ADCY5 and near CCNL1 are associated with fetal growth and birth weight<br />
Source : Nature Genetics, Volume 42, Issue 5, May <strong>2010</strong>, Pages 430-435<br />
Document Type : Article<br />
Keywords : adcy5 gene, allele, article, birth weight, CCNL1 gene, Europe, female, fetus growth, gene, genetic association, genetic<br />
variability, glucose blood level, human, LEKR1 gene, maternal smoking, non insulin dependent diabetes mellitus, priority<br />
journal, single nucleotide polymorphism, third trimester pregnancy; Adenylate Cyclase, Alleles, Birth Weight, Cohort<br />
Studies, Cyclins, Diabetes Mellitus, Type 2, Ethnic Groups, Female, Genetic Predisposition to<br />
Disease, Genotype, Glucose, Humans, Isoenzymes, Male, Models, Genetic, Pregnancy<br />
<strong>Abstract</strong> : To identify genetic variants associated with birth weight, we meta-analyzed six genome-wide association (GWA) studies (n =<br />
10,623 Europeans from pregnancy/birth cohorts) and followed up two lead signals in 13 replication studies (n = 27,591).<br />
rs900400 near LEKR1 and CCNL1 (P = 2 × 10 35) and rs9883204 in ADCY5 (P = 7 × 10 15) were robustly associated with birth<br />
weight. Correlated SNPs in ADCY5 were recently implicated in regulation <strong>of</strong> glucose levels and susceptibility to type 2<br />
diabetes, providing evidence that the well-described association between lower birth weight and subsequent type 2 diabetes<br />
has a genetic component, distinct from the proposed role <strong>of</strong> programming by maternal nutrition. Using data from both SNPs,<br />
we found that the 9% <strong>of</strong> Europeans carrying four birth weight-lowering alleles were, on average, 113g (95% CI 89-137g) lighter<br />
at birth than the 24% with zero or one alleles (P trend = 7 × 10 30). The impact on birth weight is similar to that <strong>of</strong> a mother<br />
smoking 4-5 cigarettes per day in the third trimester <strong>of</strong> pregnancy. © <strong>2010</strong> Nature America, Inc. All rights reserved.<br />
No.325<br />
Author (s) : Fried, J.R., Gibbons, R.V., Kalayanarooj, S., Thomas, S.J., Srikiatkhachorn, A., Yoon, I.-K., Jarman, R.G., Green, S., Rothman, A.L.,<br />
Cummings, D.A.T.<br />
Title : Serotype-specific differences in the risk <strong>of</strong> dengue hemorrhagic fever: An analysis <strong>of</strong> data collected in Bangkok, Thailand from<br />
1994 to 2006<br />
Source : PLoS Neglected Tropical Diseases, Volume 4, Issue 3, March <strong>2010</strong>, Article number e617<br />
Document Type : Article<br />
Keywords : adolescent, article, child, controlled study, dengue, Dengue virus, disease severity, female, human, major clinical<br />
study, male, multivariate analysis, pathophysiology, preschool child, risk assessment, school child, serotype, univariate<br />
analysis, classification, hospitalization, infant, isolation and purification, pathology, prospective<br />
study, serotyping, Thailand, virology; Adolescent, Child, Child, Preschool, Dengue Hemorrhagic Fever, Dengue<br />
Virus, Female, Humans, Infant, Male, Prospective Studies, Serotyping, Severity <strong>of</strong> Illness Index, Thailand<br />
<strong>Abstract</strong> : Background: It is unclear whether dengue serotypes differ in their propensity to cause severe disease. We analyzed<br />
differences in serotype-specific disease severity in children presenting for medical attention in Bangkok, Thailand.<br />
Methodology/Principal Findings: Prospective studies were conducted from 1994 to 2006. Univariate and multivariate logistic<br />
and multinomial logistic regressions were used to determine if dengue hemorrhagic fever (DHF) and signs <strong>of</strong> severe clinical<br />
disease (pleural effusion, ascites, thrombocytopenia, hemoconcentration) were associated with serotype. Crude and adjusted<br />
odds ratios were calculated. There were 162 (36%) cases with DENV-1, 102 (23%) with DENV-2, 123 (27%) with DENV-3, and 64<br />
(14%) with DENV-4. There was no significant difference in the rates <strong>of</strong> DHF by serotype: DENV-2 (43%), DENV-3 (39%), DENV-1<br />
(34%), DENV-4 (31%). DENV-2 was significantly associated with increased odds <strong>of</strong> DHF grade I compared to DF (OR 2.9 95% CI<br />
1.1, 8.0), when using DENV-1 as the reference. Though not statistically significant, DENV-2 had an increased odds <strong>of</strong> total DHF<br />
and DHF grades II, III, and IV. Secondary serologic response was significantly associated with DHF (OR 6.2) and increased when<br />
considering more severe grades <strong>of</strong> DHF. DENV-2 (9%) and -4 (3%) were significantly less <strong>of</strong>ten associated with primary disease<br />
than DENV-1 (28%) and -3 (33%). Restricting analysis to secondary cases, we found DENV-2 and DENV-3 to be twice as likely to<br />
result in DHF as DEN-4 (p = 0.05). Comparing study years, we found the rate <strong>of</strong> DHF to be significantly less in 1999, 2000,<br />
2004, and 2005 than in 1994, the study year with the highest percentage <strong>of</strong> DHF cases, even when controlling for other<br />
variables. Conclusions/Significance: As in other studies, we find secondary disease to be strongly associated with DHF and with<br />
119
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
more severe grades <strong>of</strong> DHF. DENV-2 appears to be marginally associated with more severe dengue disease as evidenced by a<br />
significant association with DHF grade I when compared to DENV-1. In addition, we found non-significant trends with other<br />
grades <strong>of</strong> DHF. Restricting the analysis to secondary disease we found DENV-2 and -3 to be twice as likely to result in DHF as<br />
DEN-4. Differences in severity by study year may suggest that other factors besides serotype play a role in disease severity.<br />
No.326<br />
Author (s) : Fuchareon, S., Inati, A.C., Siritanaratkul, N., Koussa, S., Taher, A., Boosalis, M.S., Thein, S.L., Wallis, W., Bobbitt, P., Thomson, A.,<br />
Johnson, E., Berenson, R.J., Perrine, S.P.<br />
Title : HQK-1001 Is Well Tolerated and Augments Hemoglobin F and Hemoglobin Levels In Patients with Beta Thalassemia<br />
Intermedia<br />
Source : BLOOD, Volume: 116, Issue: 21, Pages: 1737-1737, Published: NOV 19 <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.327<br />
Author (s) : Fugthong, A., Boonyapakron, K., Sornlek, W., Tanapongpipat, S., Eurwilaichitr, L., Pootanakit, K.<br />
Title : Biochemical characterization and in vitro digestibility assay <strong>of</strong> Eupenicillium parvum (BCC17694) phytase expressed in Pichia<br />
pastoris<br />
Source : Protein Expression and Purification, Volume 70, Issue 1, March <strong>2010</strong>, Pages 60-67<br />
Document Type : Article<br />
Keywords : Eupenicillium parvum; Phytase; Phytate; Pichia pastoris expression<br />
<strong>Abstract</strong> : A mature phytase cDNA, encoding 441 amino acids, from Eupenicillium parvum (BCC17694) was cloned into a Pichia pastoris<br />
expression vector, pPICZαA, and was successfully expressed as active extracellular glycosylated protein. The recombinant<br />
phytase contained the active site RHGXRXP and HD sequence motifs, a large α/β domain and a small α-domain that are<br />
typical <strong>of</strong> histidine acid phosphatase. Glycosylation was found to be important for enzyme activity which is most active at 50<br />
°C and pH 5.5. The recombinant phytase displayed broad substrate specificity toward p-nitrophenyl phosphate, sodium-,<br />
calcium-, and potassium-phytate. The enzyme lost its activity after incubating at 50 °C for 5 min and is 50% inhibited by 5<br />
mM Cu2+. However, the enzyme exhibits broad pH stability from 2.5 to 8.0 and is resistant to pepsin. In vitro digestibility test<br />
suggested that BCC17694 phytase is at least as effective as another recombinant phytase (r-A170) which is comparable to<br />
Natuphos, a commercial phytase, in releasing phosphate from corn-based animal feed, suggesting that BCC17694 phytase is<br />
suitable for use as phytase supplement in the animal diet. © 2009 Elsevier Inc. All rights reserved.<br />
No.328<br />
Author (s) : Funari, V.A., Krakow, D., Nevarez, L., Chen, Z., Funari, T.L., Vatanavicharn, N., Wilcox, W.R., Rimoin, D.L., Nelson, S.F., Cohn, D.H.<br />
Title : BMPER mutation in Diaphanospondylodysostosis identified by ancestral autozygosity mapping and targeted high-throughput<br />
sequencing<br />
Source : American Journal <strong>of</strong> Human Genetics, Volume 87, Issue 4, 8 October <strong>2010</strong>, Pages 532-537<br />
Document Type : Article<br />
Keywords : bone morphogenetic protein, bone morphogenetic protein binding endothelial cell percursor derived<br />
regulator, DNA, regulator protein, unclassified drug; article, autosomal recessive<br />
disorder, consanguinity, diaphanospondylodysostosis, DNA sequence, exon, gene mapping, gene targeting, genetic<br />
analysis, genetic polymorphism, genetic variability, high throughput screening, homozygosity, human, musculoskeletal<br />
disease, nonhuman, nonsense mutation, nucleotide sequence, phenotype, point mutation, priority journal, regulator<br />
gene, signal transduction; Amino Acid Sequence, Animals, Base Sequence, Bone Morphogenetic Protein<br />
2, Consanguinity, Dysostoses, Genes, Recessive, Homozygote, Humans, Mice, Molecular Sequence<br />
Data, Mutation, Pedigree, Polymorphism, Single Nucleotide, Sequence Analysis, DNA, Signal<br />
Transduction, Spine, Spondylolysis; Mus<br />
<strong>Abstract</strong> : Diaphanospondylodysostosis (DSD) is a rare, recessively inherited, perinatal lethal skeletal disorder. The low frequency and<br />
perinatal lethality <strong>of</strong> DSD makes assembling a large set <strong>of</strong> families for traditional linkage-based genetic approaches<br />
challenging. By searching for evidence <strong>of</strong> unknown ancestral consanguinity, we identified two autozygous intervals, comprising<br />
34 Mbps, unique to a single case <strong>of</strong> DSD. Empirically testing for ancestral consanguinity was effective in localizing the<br />
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causative variant, thereby reducing the genomic space within which the mutation resides. High-throughput sequence analysis<br />
<strong>of</strong> exons captured from these intervals demonstrated that the affected individual was homozygous for a null mutation in<br />
BMPER, which encodes the bone morphogenetic protein-binding endothelial cell precursor-derived regulator. Mutations in<br />
BMPER were subsequently found in three additional DSD cases, confirming that defects in BMPER produce DSD. Phenotypic<br />
similarities between DSD and Bmper null mice indicate that BMPER-mediated signaling plays an essential role in vertebral<br />
segmentation early in human development. © <strong>2010</strong> The American Society <strong>of</strong> Human Genetics. All rights reserved.<br />
No.329<br />
Author (s) : Futrakul, N., Butthep, P., Chunhakan, S., Banyatsuppasin, W., Futrakul, P.<br />
Title : Vascular homeostasis in early (normo-albuminuric) type 2 diabetic nephropathy<br />
Source : ASIAN BIOMEDICINE, Volume: 4, Issue: 6, Pages: 987-990, Published: DEC <strong>2010</strong><br />
Document Type : Article<br />
Keywords : Enhanced renal perfusion; normo-albuminuric type 2 diabetic nephropathy; vascular homeostasis; restoring renal function<br />
<strong>Abstract</strong> : Background: Renal microvascular disease and reduction in peritubular capillary flow are generally observed in type 2 diabetic<br />
nephropathy (DN). Earlier therapeutic strategy with vasodilators has improved renal function in normo-albuminuric type 2 DN.<br />
Objective: Study the mechanism <strong>of</strong> vascular homeostasis in twenty patients associated with normo-albuminuric type 2 DN.<br />
Results: Angiogenic factors were observed in normo-albuminuric type 2 DN, where vascular endothelial growth factor (VEGF),<br />
was 420 +/- 341 vs. 428 +/- 291 pg/mL (normal), and vascular endothelial growth factor - receptor 1 (VEGF-R1) was 60 +/- 12<br />
vs. 49 +/- 5 ng/mL (normal), which were not significantly different from the controls. Anti-angiogenic factors were observed in<br />
normo-albuminuric type 2 DN, where angiopoietin-2, was 2309 +/- 1125 vs. 1671 +/- 835 pg/mL (normal), and vascular<br />
endothelial growth factor - receptor 2 (VEGF-R2) was 5715 +/- 1400 vs. 6126 +/- 1060 ng/mL (normal), which were not<br />
significantly different from the controls. Conclusion: The mechanism <strong>of</strong> vascular homeostasis was adequately functional in<br />
normo-albuminuric type 2 DN. This mechanism may explain the positive response to vasodilators and improved renal<br />
function in early stage <strong>of</strong> type 2 DN following the vasodilator treatment.<br />
No.330<br />
Author (s) : Gangnonngiw, W., Kanthong, N., Flegel, T.W.<br />
Title : Successful propagation <strong>of</strong> shrimp yellow head virus in immortal mosquito cells<br />
Source : Diseases <strong>of</strong> Aquatic Organisms, Volume 90, Issue 1, 18 May <strong>2010</strong>, Pages 77-83<br />
Document Type : Article<br />
Keywords : Insect cell line; Propagation; Shrimp; Virus; YHV<br />
<strong>Abstract</strong> : Research on crustacean viruses is hampered by the lack <strong>of</strong> continuous cell lines susceptible to them. To overcome this<br />
problem, we previously challenged immortal mosquito and lepidopteran cell lines with shrimp yellow head virus (YHV),<br />
followed by serial, split-passage <strong>of</strong> whole cells, and showed that this produced cells that persistently expressed YHV antigens.<br />
To determine whether such insect cultures positive for YHV antigens could be used to infect shrimp Penaeus monodon with<br />
YHV, culture supernatants and whole-cell homogenates were used to challenge shrimp by injection. Shrimp injected with<br />
culture supernatants could not be infected. However, shrimp injectionchallenged with whole-cell homogenates from Passage<br />
5 (early-passage) <strong>of</strong> such cultures died with histological and clinical signs typical for yellow head disease (YHD), while<br />
homogenates <strong>of</strong> mock-passaged, YHV-challenged cells did not. By contrast, shrimp challenged with cell homogenates <strong>of</strong> latepassage<br />
cultures became infected with YHV, but survived, suggesting that YHV attenuation had occurred during its long-term<br />
serial passage in insect cells. Thus, YHV could be propagated successfully in C6/36 mosquito cells and used at low passage<br />
numbers as a source <strong>of</strong> inoculum to initiate lethal infections in shrimp. This partially solves the problem <strong>of</strong> lack <strong>of</strong> continuous<br />
shrimp cell lines for cultivation <strong>of</strong> YHV. © Inter-Research <strong>2010</strong>.<br />
No.331<br />
Author (s) : Gangnonngiw, W., Laisutisan, K., Sriurairatana, S., Senapin, S., Chuchird, N., Limsuwan, C., Chaivisuthangkura, P., Flegel, T.W.<br />
Title : Monodon baculovirus (MBV) infects the freshwater prawn Macrobrachium rosenbergii cultivated in Thailand<br />
Source : Virus Research, Volume 148, Issue 1-2, March <strong>2010</strong>, Pages 24-30<br />
Document Type : Article<br />
Keywords : Bacilliform virus; Electron microscopy; Histopathology; Macrobrachium rosenbergii; MBV; Monodon<br />
baculovirus; PemoNPV; Penaeus monodon nucleopolyhedrovirus<br />
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<strong>Abstract</strong> : Field specimens <strong>of</strong> post-larvae <strong>of</strong> the giant freshwater prawn (Macrobrachium rosenbergii) from Thailand showed<br />
hepatopancreatic tubule epithelial cells that contained central, eosinophilic inclusions within enlarged nuclei and marginated<br />
chromatin. These inclusions resembled those produced by some baculoviruses prior to formation <strong>of</strong> occlusion bodies that<br />
enclose virions in a polyhedrin protein matrix. By electron microscopy, the intranuclear inclusions contained bacilliform,<br />
enveloped virions (approximately 327 ± 29 nm × 87 ± 12 nm) with evenly dense, linear nucleocapsids surrounded by<br />
trilaminar envelopes with lateral pockets containing nucleoproteinic filaments. In some cases, these were accompanied by<br />
moderately electron dense, spherical particles <strong>of</strong> approximately 20 nm diameter resembling polyhedrin subunits <strong>of</strong> occlusion<br />
bodies (OB) <strong>of</strong> a bacilliform virus <strong>of</strong> the black tiger shrimp Penaeus monodon, previously reported from Thailand and called<br />
monodon baculovirus (MBV). It is currently listed by the <strong>International</strong> Committee on Taxonomy <strong>of</strong> viruses as Penaeus<br />
monodon nucleopolyhedrovirus (PemoNPV). Two polymerase chain reaction (PCR) assays for MBV gave positive results with<br />
DNA extracts prepared from M. rosenbergii samples using the hot phenol technique. One <strong>of</strong> these assays targeted the<br />
polyhedrin gene <strong>of</strong> MBV to which the resulting amplicon showed 100% sequence identity. Presence <strong>of</strong> the Penaeus monodon<br />
virus polyhedrin gene was confirmed by in situ hybridization assays and by positive immunohistochemical reactions in one<br />
sample batch. The data revealed that MBV can be found but may rarely produce polyhedrin occlusion bodies in M.<br />
rosenbergii. © 2009 Elsevier B.V. All rights reserved.<br />
No.332<br />
Author (s) : Garusi, C., Lohsiriwat, V., De Lorenzi, F., Manconi, A., De Fiori, E., Bellomi, M.<br />
Title : A subfascial variant <strong>of</strong> the deep inferior epigastric artery demonstrated by preoperative multidetector computed tomographic<br />
angiography: A case report<br />
Source : Microsurgery, Volume 30, Issue 2, <strong>2010</strong>, Pages 156-158<br />
Document Type : Article<br />
Keywords : adult, angiography, article, case report, deep inferior epigastric perforator flap, end to end anastomosis, fascia, female, follow<br />
up, human, image quality, incision, intraoperative period, mastectomy, multidetector computed tomography, preoperative<br />
evaluation, priority journal, radiodiagnosis, rectus abdominis muscle; Abdominal Wall, Adult, Breast<br />
Neoplasms, Carcinoma, Epigastric Arteries, Fascia, Female, Humans, Mammaplasty, Mastectomy, Microsurgery, Preoperative<br />
Care, Surgical Flaps, Tomography, X-Ray Computed<br />
<strong>Abstract</strong> : Precise preoperative imaging by multidetector computed tomographic (MDCT) angiography for planning <strong>of</strong> deep inferior<br />
epigastric artery perforator (DIEP) flap dissection has been reported for enormous advantages in terms <strong>of</strong> reduced operative<br />
time and minimized flap-related complications. This case report shows a particularly rare anatomical subfascia variant <strong>of</strong> deep<br />
inferior epigastric artery (DIEA) which can be preoperatively demonstrated by MDCT angiogram. Therefore, the intraoperative<br />
finding also confirms the radiologic data and results in meticulous flap harvesting during incision on anterior rectus sheath.<br />
Additionally, the authors emphasize on performing preoperative high quality imaging for DIEP intervention precisely for<br />
specific vulnerable course <strong>of</strong> subfascial plane DIEP, which is rare but tends to be at risk without foreknowing its exact course.<br />
© 2009 Wiley-Liss, Inc.<br />
No.333<br />
Author (s) : Gaudin, K., Millet, P., Fawaz, F., Olliaro, P., White, N.J., Cassus-Coussère, C., Agbahoungha, U., Dubost, J.-P.<br />
Title : Investigation <strong>of</strong> porous graphitic carbon at high-temperature liquid chromatography with evaporative light scattering detection<br />
for the analysis <strong>of</strong> the drug combination artesunate-Azithromycin for the treatment <strong>of</strong> severe malaria<br />
Source : Journal <strong>of</strong> Chromatography A, Volume 1217, Issue 1, 1 January <strong>2010</strong>, Pages 75-81<br />
Document Type : Article<br />
Keywords : Artesunate; Azithromycin; Evaporative light scattering detection; HTLC-PGC-ELSD; Malaria; Porous graphitic carbon<br />
<strong>Abstract</strong> : Artesunate combined therapies represent the best option for the treatment <strong>of</strong> malaria and require the development <strong>of</strong> new<br />
methods <strong>of</strong> analysis. Retention, selectivity and detection with high-temperature liquid chromatography-porous graphitic<br />
carbon-evaporative light scattering detection was studied for artesunate and azithromycin separation. Organic solvent,<br />
concentration <strong>of</strong> organic modifiers, temperature and flow rate were all relevant parameters to optimize this separation. The<br />
behaviour <strong>of</strong> artesunate in the tested conditions appeared close to a neutral compound. In CH3OH, only azithromycin<br />
retention was dramatically altered depending on the [triethylamine]/[formic acid] ratio and on the temperature, whereas in<br />
CH3CN, azithromycin, artesunate, artemisinin and dihydroartemisinin retentions decreased with the temperature increase<br />
whatever the organic modifier ratio. The best efficiency was obtained with CH3CN. 25% variation <strong>of</strong> the concentration values<br />
<strong>of</strong> the organic modifiers did not significantly influenced the retention. The sensitivity <strong>of</strong> ELSD increased with the flow rate<br />
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decrease. Peak area and S/N ratio dramatically decreased with the flow rate increase by 10- and 5-fold for artesunate and<br />
azithromycin, respectively. Non-linear calibration curves were obtained for both artesunate and azithromycin. © 2009 Elsevier<br />
B.V. All rights reserved.<br />
No.334<br />
Author (s) : Gauthier, S., Garcia, A., Sano, M., Robert, P., Senanarong, V., Woodward, M., Brodaty, H.<br />
Title : Priorities for research consortia on Alzheimer's disease<br />
Source : Alzheimer's and Dementia, Volume 6, Issue 4, July <strong>2010</strong>, Pages 359-362<br />
Document Type : Short Survey<br />
Keywords : Alzheimer disease, cultural anthropology, dementia, disease registry, family, human, liaison psychiatry, longitudinal<br />
study, medical education, medical ethics, medical research, observational study, outcome assessment, prevention<br />
study, priority journal, program development, short survey, validation process; Age <strong>of</strong> Onset, Alzheimer Disease, Clinical Trials<br />
as Topic, Data Interpretation, Statistical, Ethics, Research, Humans, Longitudinal Studies, Randomized Controlled Trials as<br />
Topic, Registries, Reproducibility <strong>of</strong> Results, Research, Research Design<br />
<strong>Abstract</strong> : Coordination and harmonization <strong>of</strong> efforts between five major research consortia on Alzheimer's disease may increase our<br />
understanding <strong>of</strong> this condition and improve our therapeutic approaches. Specific opportunities include a registry for families<br />
with early onset dementia, a study registry, minimal data sets, validation <strong>of</strong> assessment tools and outcomes, update on<br />
ethical issues, resolution <strong>of</strong> methodological issues, new investigators training, longitudinal observation studies, prevention<br />
studies, and liaison with stakeholders such as Alzheimer Disease <strong>International</strong>. © <strong>2010</strong> The Alzheimer's Association. All rights<br />
reserved.<br />
No.335<br />
Author (s) : Geibprasert, S., Krings, T.<br />
Title : Response<br />
Source : Journal <strong>of</strong> Neurosurgery, Volume 112, Issue 3, March <strong>2010</strong>, Page 696<br />
Document Type : Letter<br />
Keywords : anastomosis, brain arteriovenous malformation, human, hydrocephalus, letter, postural drainage, priority journal, shunting<br />
<strong>Abstract</strong> : –<br />
No.336<br />
Author (s) : Geibprasert, S., Krings, T., Apitzsoh, J., Reinges, M.H.T., Nolte, K.W., Hans, F.J.<br />
Title : Subarachnoid hemorrhage following posterior spinal artery aneurysm: A case report and review <strong>of</strong> the literature<br />
Source : Interventional Neuroradiology, Volume 16, Issue 2, June <strong>2010</strong>, Pages 183-190<br />
Document Type : Article<br />
Keywords : Arterial dissection; Posterior spinal artery; Spinal aneurysm; Spinal subarachnoid hemorrhage<br />
<strong>Abstract</strong> : Isolated posterior spinal artery aneurysms are rare vascular lesions. We describe the case <strong>of</strong> a 43-year-old man presenting<br />
with spinal subarachnoid hemorrhage after a minor trauma who was found to have a dissecting aneurysm <strong>of</strong> a posterior spinal<br />
artery originating from the right T4 level. Endovascular treatment was not contemplated because <strong>of</strong> the small size <strong>of</strong> the<br />
feeding artery, whereas surgical resection was deemed more appropriate because <strong>of</strong> the posterolateral perimedullary location<br />
that was well appreciated on CT angiography. After surgical resection <strong>of</strong> the aneurysm the patient had a complete<br />
neurological recovery. In comparison to anterior spinal artery aneurysms whose pathogenesis is diverse, posterior spinal<br />
aneurysms are most <strong>of</strong>ten secondary to a dissection and represent false or spurious aneurysms. Although the definite<br />
diagnosis still requires spinal angiography, MRI and CT may better delineate the relationship <strong>of</strong> the aneurysm to the spinal<br />
cord in order to determine the best treatment method. Prompt treatment is recommended as they have high rebleeding and<br />
mortality rates.<br />
No.337<br />
Author (s) : Geibprasert, S., Pongpech, S., Jiarakongmun, P., Shr<strong>of</strong>f, M.M., Armstrong, D.C., Krings, T.<br />
Title : Radiologic assessment <strong>of</strong> brain arteriovenous malformations: What clinicians need to know<br />
Source : Radiographics, Volume 30, Issue 2, March <strong>2010</strong>, Pages 483-501<br />
Document Type : Article<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : article, brain angiography, brain arteriovenous malformation, computer assisted tomography, human, magnetic resonance<br />
angiography, methodology; Cerebral Angiography, Humans, Intracranial Arteriovenous Malformations, Magnetic Resonance<br />
Angiography, Tomography, X-Ray Computed<br />
<strong>Abstract</strong> : Brain arteriovenous malformations (AVMs) are abnormal vascular connections within the brain that are presumably congenital<br />
in nature. There are several subgroups, the most common being glomerular type brain AVMs, with fistulous type AVMs being<br />
less common. A brain AVM may also be a part <strong>of</strong> more extensive disease (eg, cerebr<strong>of</strong>acial arteriovenous metameric<br />
syndrome). When intracranial pathologic vessels are encountered at cross-sectional imaging, other diagnoses must also be<br />
considered, including large developmental venous anomalies, malignant dural arteriovenous fistulas, and moyamoya disease,<br />
since these entities are known to have different natural histories and require different treatment options. Several imaging<br />
findings in brain AVMs have an impact on decision making with respect to clinical management; the most important are those<br />
known to be associated with risk <strong>of</strong> future hemorrhage, including evidence <strong>of</strong> previous hemorrhage, intranidal aneurysms,<br />
venous stenosis, deep venous drainage, and deep location <strong>of</strong> the nidus. Other imaging findings that should be included in the<br />
radiology report are secondary effects caused by brain AVMs that may lead to nonhemorrhagic neurologic deficits, such as<br />
venous congestion, gliosis, hydrocephalus, or arterial steal. © RSNA, <strong>2010</strong>.<br />
No.338<br />
Author (s) : Geibprasert, S.; Fanning, N.F.; Pedroza, A.; terBrugge, K.G.<br />
Title : Imaging characteristics <strong>of</strong> arteriovenous malformations simulating vascular tumors <strong>of</strong> the temporalis muscle: a report <strong>of</strong> two<br />
cases<br />
Source : INTERNATIONAL JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY, Volume: 39, Issue: 4, Pages: 402-406, DOI:<br />
10.1016/j.ijom.2009.12.009, Published: APR <strong>2010</strong><br />
Document Type : Article<br />
Keywords : temporalis muscle; facial; scalp; intramuscular; arteriovenous malformation<br />
<strong>Abstract</strong> : Arteriovenous malformations (AVMs) <strong>of</strong> the temporalis muscle are an extremely rare subgroup <strong>of</strong> head and neck AVMs. The<br />
authors report two cases presenting with temporal fossa tumor-like lesions. MRI demonstrated enlargement <strong>of</strong> the temporalis<br />
muscle with hypointensity on T1, hyperintensity on T2 and moderate contrast enhancement. Angiography shows slow<br />
arteriovenous shunting with a dense capillary blush <strong>of</strong> the entire muscle before draining into facial veins. The arterial supply<br />
is characteristic <strong>of</strong> being restricted to the temporalis muscle.<br />
No.339<br />
Author (s) : Geibprasert, Sasikhan; Krings, Timo<br />
Title : Hydrocephalus in unruptured brain arteriovenous malformations RESPONSE<br />
Source : JOURNAL OF NEUROSURGERY, Volume: 112, Issue: 3, Pages: 696-696, Published: MAR <strong>2010</strong><br />
Document Type : Letter<br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.340<br />
Author (s) : Gerdprasert, S., Pruksacheva, T., Panijpan, B., Ruenwongsa, P.<br />
Title : Development <strong>of</strong> a web-based learning medium on mechanism <strong>of</strong> labour for nursing students<br />
Source : Nurse Education Today, Volume 30, Issue 5, July <strong>2010</strong>, Pages 464-469<br />
Document Type : Article<br />
Keywords : Mechanism <strong>of</strong> labour; Multimedia; Nursing students; Web-based learning unit<br />
<strong>Abstract</strong> : This study aimed to develop a web-based learning media on the process and mechanism <strong>of</strong> labour for the third-year<br />
university nursing and midwifery students. This media was developed based on integrating principles <strong>of</strong> the mechanism <strong>of</strong><br />
labour with the 5Es inquiry cycle and interactive features <strong>of</strong> information technology. In this study, the web-based learning unit<br />
was used to supplement the conventional lecture as in the traditional teaching. Students' achievements were assessed by<br />
using the pre- and post-test on factual knowledge and semi-structured interviews on attitude to the unit. Supplementation<br />
with this learning unit made learning significantly more effective than the traditional lecture by itself. The students also<br />
showed positive attitude toward the learning unit. © 2009 Elsevier Ltd.<br />
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No.341<br />
Author (s) : Gerdsri, N., Assakul, P., Vatananan, R.S.<br />
Title : An activity guideline for technology roadmapping implementation<br />
Source : Technology Analysis and Strategic Management, 22 (2), pp. 229-242.<br />
Document Type : Article<br />
Keywords : Activity guideline; Change management; Echnology roadmapping; TRM; TRM implementation<br />
<strong>Abstract</strong> : To strive for sustainability under today's intense business competition, organisations apply technology roadmapping (TRM) as<br />
a strategic planning tool to align their technology strategies with business strategies. Many organisations desire to integrate<br />
TRM into an ongoing strategic planning process. The consequences <strong>of</strong> TRM implementation can lead to some changes in the<br />
business process, organisational structure, or even working culture. Applying a change management approach will help<br />
organisations to understand the basic elements that an individual needs so that some challenges can be addressed in<br />
advance before adopting the TRM process. This paper proposes a practical guideline to implement technology roadmapping<br />
along with a case example. © <strong>2010</strong> Taylor & Francis.<br />
No.342<br />
Author (s) : Gesprasert, G., Wichukchinda, N., Mori, M., Shiino, T., Auwanit, W., Sriwanthana, B., Pathipvanich, P., Sawanpanyalert, P., Miura,<br />
T., Auewarakul, P., Thitithanyanont, A., Ariyoshi, K.<br />
Title : HLA-Associated immune pressure on gag protein in CRF01_AE-Infected individuals and its association with plasma viral load<br />
Source : PLoS ONE, Volume 5, Issue 6, <strong>2010</strong>, Article number e11179<br />
Document Type : Article<br />
Keywords : amino acid, capsid protein, Gag protein, HLA antigen, primer DNA; article, CD4 lymphocyte count, cytotoxic T<br />
lymphocyte, gene amplification, gene frequency, gene mutation, gene sequence, genetic polymorphism, HLA<br />
typing, human, Human immunodeficiency virus 1 infection, major clinical study, nonhuman, nucleotide sequence, polymerase<br />
chain reaction, revertant, sequence analysis, Thailand, unindexed sequence, virus load, allele, disease<br />
transmission, genetics, Human immunodeficiency virus infection, immunology, mutation, virology; Human immunodeficiency<br />
virus, Human immunodeficiency virus 1, Miridae; Alleles, Base Sequence, DNA Primers, Gene Products, gag, HIV Infections, HLA<br />
Antigens, Mutation, Polymerase Chain Reaction, T-Lymphocytes, Cytotoxic, Viral Load<br />
<strong>Abstract</strong> : Background: The human leukocyte antigen (HLA)-restricted cytotoxic T-lymphocyte (CTL) immune response is one <strong>of</strong> the<br />
major factors determining the genetic diversity <strong>of</strong> human immunodeficiency virus (HIV). There are few population-based<br />
analyses <strong>of</strong> the amino acid variations associated with the host HLA type and their clinical relevance for the Asian population.<br />
Here, we identified HLA-associated polymorphisms in the HIV-1 CRF01_AE Gag protein in infected married couples, and<br />
examined the consequences <strong>of</strong> these HLA-selected mutations after transmission to HLA-unmatched recipients.<br />
Methodology/Principal Findings: One hundred sixteen HIV-1-infected couples were recruited at a government hospital in<br />
northern Thailand. The 1.7-kb gag gene was amplified and directly sequenced. We identified 56 associations between amino<br />
acid variations in Gag and HLA alleles. Of those amino acid variations, 35 (62.5%) were located within or adjacent to regions<br />
reported to be HIV-specific CTL epitopes restricted by the relevant HLA. Interestingly, a significant number <strong>of</strong> HLAassociated<br />
amino acid variations appear to be unique to the CRF01_AE-infected Thai population. Variations in the capsid protein (p24)<br />
had the strongest associations with the viral load and CD4 cell count. The mutation and reversion rates after transmission to a<br />
host with a different HLA environment varied considerably. The p24 T242N variant escape from B57/58 CTL had a significant<br />
impact on the HIV-1 viral load <strong>of</strong> CRF01_AE-infected patients. Conclusions/Significance: HLA-associated amino acid mutations<br />
and the CTL selection pressures on the p24 antigen appear to have the most significant impact on HIV replication in a<br />
CRF01_AE-infected Asian population. HLA-associated mutations with a low reversion rate accumulated as a footprint in this<br />
Thai population. The novel HLA-associated mutations identified in this study encourage us to acquire more extensive<br />
information about the viral dynamics <strong>of</strong> HLA-associated amino acid polymorphisms in a given population as effective CTL<br />
vaccine targets. © <strong>2010</strong> Gesprasert et al.<br />
No.343<br />
Author (s) : Glover, S.J., Maude, R.J., Taylor, T.E., Molyneux, M.E., Beare, N.A.<br />
Title : Malarial retinopathy and fluorescein angiography findings in a Malawian child with cerebral malaria<br />
Source : The Lancet Infectious Diseases, Volume 10, Issue 6, June <strong>2010</strong>, Page 440<br />
Document Type : Note<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : quinine; anemia, blood transfusion, brain malaria, case report, child, coma, human, malarial<br />
retinopathy, Malawi, male, note, ophthalmoscopy, papilledema, Plasmodium falciparum, preschool child, priority<br />
journal, retina fluorescein angiography, retina hemorrhage, retinopathy; Fluorescein Angiography, Humans, Infant, Malaria,<br />
Cerebral, Malawi, Male, Retinal Diseases<br />
<strong>Abstract</strong> : –<br />
No.344<br />
Author (s) : Goh, H. G.; Kim, D.; Choi, S. Y.; Kim, S. H.; Kim, J. Y.; Park, S.; Kim, M. S.; Jang, E. J.; Woodman, R.; Szczudlo, T.; Kim, H. J.; Jootar,<br />
S.; Kim, H. J.; Sohn, S. K.; Park, J. S.; Kim, S. H.; Zang, D. Y.; Oh, S. J.; Kim, D. W.<br />
Title : EFFICACY OF NILOTINIB VERSUS IMATINIB IN ADULT PATIENTS WITH PH plus CHRONIC MYELOID LEUKEMIA IN EARLY CP WHO<br />
HAVE A SUBOPTIMAL MOLECULAR RESPONSE TO IMATINIB (RE-NICE MULTICENTER STUDY)<br />
Source : HAEMATOLOGICA-THE HEMATOLOGY JOURNAL, Volume: 95, Supplement: 2, Pages: 538-538, Meeting <strong>Abstract</strong>: 1328,<br />
Published: JUN <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.345<br />
Author (s) : Goldhaber-Fiebert, J.D., Li, H., Ratanawijitrasin, S., Vidyasagar, S., Wang, X.Y., Aljunid, S., Shah, N., Wang, Z., Hirunrassamee, S.,<br />
Bairy, K.L., Wang, J., Saperi, S., Nur, A.M., Eggleston, K.<br />
Title : Inpatient treatment <strong>of</strong> diabetic patients in Asia: Evidence from India, China, Thailand and Malaysia<br />
Source : Diabetic Medicine, Volume 27, Issue 1, January <strong>2010</strong>, Pages 101-108<br />
Document Type : Article<br />
Keywords : Asia; Diabetes; Economic burden; Inpatient treatment<br />
<strong>Abstract</strong> : Aims: The prevalence <strong>of</strong> Type 2 diabetes mellitus (DM) has grown rapidly, but little is known about the drivers <strong>of</strong> inpatient<br />
spending in low- and middle-income countries. This study aims to compare the clinical presentation and expenditure on<br />
hospital admission for inpatients with a primary diagnosis <strong>of</strong> Type 2 DM in India, China, Thailand and Malaysia. Methods We<br />
analysed data on adult, Type 2 DM patients admitted between 2005 and 2008 to five tertiary hospitals in the four countries,<br />
reporting expenditures relative to income per capita in 2007. Results Hospital admission spending for diabetic inpatients with<br />
no complications ranged from 11 to 75% <strong>of</strong> per-capita income. Spending for patients with complications ranged from 6% to<br />
over 300% more than spending for patients without complications treated at the same hospital. Glycated haemoglobin was<br />
significantly higher for the uninsured patients, compared with insured patients, in India (8.6 vs. 8.1%), Hangzhou, China (9.0 vs.<br />
8.1%), and Shandong, China (10.9 vs. 9.9%). When the hospital admission expenditures <strong>of</strong> the insured and uninsured patients<br />
were statistically different in India and China, the uninsured always spent less than the insured patients. Conclusions With the<br />
rising prevalence <strong>of</strong> DM, households and health systems in these countries will face greater economic burdens. The returns to<br />
investment in preventing diabetic complications appear substantial. Countries with large out-<strong>of</strong>-pocket financing burdens such<br />
as India and China are associated with the widest gaps in resource use between insured and uninsured patients. This probably<br />
reflects both overuse by the insured and underuse by the uninsured. © <strong>2010</strong> Diabetes UK.<br />
No.346<br />
Author (s) : Good, D.M., Zürbig, P., Argilés, À., Bauer, H.W., Behrens, G., Coon, J.J., Dakna, M., Decramer, S., Delles, C., Dominiczak, A.F.,<br />
Ehrich, J.H.H., Eitner, F., Fliser, D., Frommberger, M., Ganser, A., Girolami, M.A., Golovko, I., Gwinner, W., Haubitz, M., Herget-<br />
Rosenthal, S., Jankowski, J., Jahn, H., Jerums, G., Julian, B.A., Kellmann, M., Kliem, V., Kolch, W., Krolewski, A.S., Luppi, M.,<br />
Massy, Z., Melter, M., Neusüss, C., Novak, J., Peter, K., Rossing, K., Rupprecht, H., Schanstra, J.P., Schiffer, E., Stolzenburg, J.-U.,<br />
Tarnow, L., Theodorescu, D., Thongboonkerd, V., Vanholder, R., Weissinger, E.M., Mischak, H., Schmitt-Kopplinr, P.<br />
Title : Naturally occurring human urinary peptides for use in diagnosis <strong>of</strong> chronic kidney disease<br />
Source : Molecular and Cellular Proteomics, Volume 9, Issue 11, November <strong>2010</strong>, Pages 2424-2437<br />
Document Type : Article<br />
Keywords : biological marker; adult, aged, amino acid sequence, article, capillary electrophoresis, chronic kidney disease, diagnostic<br />
accuracy, female, human, major clinical study, male, mass spectrometry, priority journal, protein urine<br />
level, proteomics, sensitivity and specificity, urinalysis; Adult, Aged, Biological Markers, Databases, Factual, Electrophoresis,<br />
126
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Capillary, Female, Humans, Kidney Failure, Chronic, Male, Mass Spectrometry, Middle Aged, Peptides, Proteomics, ROC<br />
Curve, Young Adult<br />
<strong>Abstract</strong> : Because <strong>of</strong> its availability, ease <strong>of</strong> collection, and correlation with physiology and pathology, urine is an attractive source for<br />
clinical proteomics/peptidomics. However, the lack <strong>of</strong> comparable data sets from large cohorts has greatly hindered the<br />
development <strong>of</strong> clinical proteomics. Here, we report the establishment <strong>of</strong> a reproducible, high resolution method for<br />
peptidome analysis <strong>of</strong> naturally occurring human urinary peptides and proteins, ranging from 800 to 17,000 Da, using samples<br />
from 3,600 individuals analyzed by capillary electrophoresis coupled to MS. All processed data were deposited in an<br />
Structured Query Language (SQL) database. This database currently contains 5,010 relevant unique urinary peptides that serve<br />
as a pool <strong>of</strong> potential classifiers for diagnosis and monitoring <strong>of</strong> various diseases. As an example, by using this source <strong>of</strong><br />
information, we were able to define urinary peptide biomarkers for chronic kidney diseases, allowing diagnosis <strong>of</strong> these<br />
diseases with high accuracy. Application <strong>of</strong> the chronic kidney disease-specific biomarker set to an independent test cohort in<br />
the subsequent replication phase resulted in 85.5% sensitivity and 100% specificity. These results indicate the potential<br />
usefulness <strong>of</strong> capillary electrophoresis coupled to MS for clinical applications in the analysis <strong>of</strong> naturally occurring urinary<br />
peptides. © <strong>2010</strong> by The American Society for Biochemistry and Molecular Biology, Inc.<br />
No.347<br />
Author (s) : Govitrapong, P., Boontem, P., Kooncumchoo, P., Pinweha, S., Namyen, J., Sanvarinda, Y., Vatanatunyakum, S.<br />
Title : Increased blood oxidative stress in amphetamine users<br />
Source : Addiction Biology, Volume 15, Issue 1, January <strong>2010</strong>, Pages 100-102<br />
Document Type : Article<br />
Keywords : Amphetamine; Catalase; Glutathione peroxidase; Lipid peroxidation; Oxidative stress; Superoxide dismutase<br />
<strong>Abstract</strong> : Amphetamine derivatives have been shown to be a potential brain neurotoxin based on the production <strong>of</strong> free radicals that<br />
occurs after administration. The purpose <strong>of</strong> this study was to examine the lipid peroxidation and antioxidant enzymes in the<br />
blood <strong>of</strong> amphetamine users. The plasma lipid peroxidation was determined and reported as thiobarbituric acid reactive<br />
substance and was significantly increased (+21%), whereas the activities <strong>of</strong> the erythrocyte antioxidant enzymes glutathione<br />
peroxidase, catalase, and superoxide dismutase were significantly decreased (-32%, -14% and -31%, respectively) in<br />
amphetamine users. These results implicated the potential role <strong>of</strong> oxidative stress in amphetamine-induced neurotoxicity. ©<br />
2009 Society for the Study <strong>of</strong> Addiction.<br />
No.348<br />
Author (s) : Grace, S.R., Agarwal, R.P., Kaymakçalan, B., Sae-Jie, W.<br />
Title : Oscillation theorems for second order nonlinear dynamic equations<br />
Source : Journal <strong>of</strong> Applied Mathematics and Computing, Volume 32, Issue 1, February <strong>2010</strong>, Pages 205-218<br />
Document Type : Article<br />
Keywords : Almost linear; Nonoscillation; Oscillation; Sublinear; Superlinear<br />
<strong>Abstract</strong> : Some new criteria for the oscillation <strong>of</strong> nonlinear dynamic equations <strong>of</strong> the form (a(t)(x ∆(t))α)∆ + f(t,x σ(t))=0 on a time<br />
scale T are established. © 2009 Korean Society for Computational and Applied Mathematics.<br />
No.349<br />
Author (s) : Grace, S.R., Agarwal, R.P., Wichuta, S.-J.<br />
Title : Monotone and oscillatory behavior <strong>of</strong> certain fourth order nonlinear dynamic equations<br />
Source : Dynamic Systems and Applications, Volume 19, Issue 1, March <strong>2010</strong>, Pages 25-32<br />
Document Type : Article<br />
Keywords : Dynamic equation; Nonoscillation; Oscillation<br />
<strong>Abstract</strong> : Monotone and oscillatory behavior <strong>of</strong> solutions <strong>of</strong> the fourth order dynamic equation (a(x∆∆)α) ∆∆(t)+(t)(xσ)β(t) = 0 with<br />
the property that x(t)/∫tt0 ∫ at0a-1/a(τ)∆τ∆s → as t → ∞ are established. © Dynamic Publishers, Inc.<br />
No.350<br />
Author (s) : Graves, N., Harbarth, S., Beyersmann, J., Barnett, A., Halton, K., Cooper, B.<br />
Title : Estimating the cost <strong>of</strong> health care-assodated infections: Mind your p's and q's<br />
Source : Clinical Infectious Diseases, Volume 50, Issue 7, 1 April <strong>2010</strong>, Pages 1017-1021<br />
Document Type : Review<br />
127
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : decision making, funding, health care cost, health economics, hospitalization, human, infection, infection<br />
control, information, length <strong>of</strong> stay, outcome assessment, priority journal, review; Costs and Cost Analysis, Cross<br />
Infection, Health Care Costs, Humans, Models, Economic<br />
<strong>Abstract</strong> : Monetary valuations <strong>of</strong> the economic cost <strong>of</strong> health care-associated infections (HAIs) are important for decision making and<br />
should be estimated accurately. Erroneously high estimates <strong>of</strong> costs, designed to jolt decision makers into action, may do<br />
more harm than good in the struggle to attract funding for infection control. Expectations among policy makers might be<br />
raised, and then they are disappointed when the reduction in the number <strong>of</strong> HAIs does not yield the anticipated cost saving.<br />
For this article, we critically review the field and discuss 3 questions. Why measure the cost <strong>of</strong> an HAI? What outcome should<br />
be used to measure the cost <strong>of</strong> an HAI? What is the best method for making this measurement? The aim is to encourage<br />
researchers to collect and then disseminate information that accurately guides decisions about the economic value <strong>of</strong><br />
expanding or changing current infection control activities. © <strong>2010</strong> by the Infectious Diseases Society <strong>of</strong> America. All rights<br />
reserved.<br />
No.351<br />
Author (s) : Green, J.H.<br />
Title : Who's the boss?: Culture and the problem <strong>of</strong> transfer<br />
Source : <strong>International</strong> Journal <strong>of</strong> Learning, Volume 17, Issue 6, <strong>2010</strong>, Pages 393-402<br />
Document Type : Article<br />
Keywords : Culture; Higher education; Learner agency; Personal epistemology; Transfer <strong>of</strong> learning<br />
<strong>Abstract</strong> : Transfer <strong>of</strong> learning, although arguably education's most important goal, is also its most pervasive problem. Research confirms<br />
one <strong>of</strong> the classroom teacher's most common complaints: that learners do not transfer what they have learnt in one subject<br />
to another, or fail to use what they have learnt in the school setting in the "real world." There may be many reasons for this -<br />
one being that transfer is <strong>of</strong>ten left to take care <strong>of</strong> itself. To counter this, cognitive-based strategies, such as "hugging" and<br />
"bridging," suggest that educators pay active attention to both structural and instructional elements in order to effect transfer:<br />
educators, can, for example, draw learners' attention to extra-contextual opportunities to use their learning, or coach them to<br />
develop the thinking skills that allow the abstraction <strong>of</strong> transferrable principles. Conceptualizations such as these, however,<br />
do not go far enough. Because these interventions rely on the instructor, the sought-after transfer may not occur once the<br />
learner is removed from the context and is required to act independently. What is preferred is a self-motivated, autonomous,<br />
mastery-oriented learner - one who will actively seek opportunities using his or her skills or knowledge in new contexts. Such<br />
a learner, however, will only develop if cultural conditions so allow. This discussion explores the link between culture,<br />
learner agency, and transfer <strong>of</strong> learning. In so doing, it examines practical cultural concerns, particularly in a Thai context, that<br />
may help or hinder transfer <strong>of</strong> learning. © Common Ground, Jonathan H. Green.<br />
No.352<br />
Author (s) : Gritsanapan, W.; Sakulpanich, A.<br />
Title : Standardized Cassia fistula pod extracts<br />
Source : PLANTA MEDICA, Volume: 76, Issue: 12, Pages: 1231-1231, Published: AUG <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.353<br />
Author (s) : Gritsanapan, W.; Sakulpanich, A.; Thongpraditchote, S.<br />
Title : Anthraquinone content and toxicity test <strong>of</strong> Cassia fistula pod extracts<br />
Source : PLANTA MEDICA, Volume: 76, Issue: 12, Pages: 1225-1225, Published: AUG <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.354<br />
Author (s) : Gritsanapan, W.; Tangyuenyongwatana, P.<br />
Title : Investigation <strong>of</strong> Thai traditional way for detoxification <strong>of</strong> aloe<br />
128
Source : PLANTA MEDICA, Volume: 76, Issue: 12, Pages: 1216-1216, Published: AUG <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.355<br />
Author (s) : Gruson, K.I., Pillai, G., Vanadurongwan, B., Parsons, B.O., Flatow, E.L.<br />
Title : Early clinical results following staged bilateral primary total shoulder arthroplasty<br />
Source : Journal <strong>of</strong> Shoulder and Elbow Surgery, Volume 19, Issue 1, January <strong>2010</strong>, Pages 137-142<br />
Document Type : Article<br />
Keywords : adult, aged, article, blood transfusion, clinical article, clinical evaluation, daily life activity, female, follow<br />
up, hospitalization, human, male, operation duration, outcome assessment, pain assessment, patient<br />
satisfaction, postoperative hemorrhage, priority journal, range <strong>of</strong> motion, retrospective study, scoring system, Short Form<br />
36, shoulder arthroplasty, surgeon, visual analog scale; Aged, Aged, 80 and over, Arthroplasty, Replacement, Chi-Square<br />
Distribution, Cohort Studies, Female, Follow-Up Studies, Humans, Length <strong>of</strong> Stay, Male, Middle Aged, Pain<br />
Measurement, Patient Satisfaction, Postoperative Complications, Probability, Range <strong>of</strong> Motion, Articular, Recovery <strong>of</strong><br />
Function, Retrospective Studies, Risk Assessment, Shoulder Joint, Shoulder Pain, Time Factors, Treatment Outcome<br />
<strong>Abstract</strong> : Background: The advantages <strong>of</strong> performing either a single- or 2-staged joint replacement has been reviewed extensively in the<br />
hip and knee arthroplasty literature, but far less data exist regarding total shoulder replacements. In the appropriate clinical<br />
setting, bilateral total shoulder arthroplasty yields excellent functional results with a low complication pr<strong>of</strong>ile. Materials and<br />
methods: We evaluated retrospectively the records <strong>of</strong> 13 consecutive patients (26 shoulders) who underwent staged bilateral<br />
primary total shoulder replacements by a single surgeon, with a minimum follow-up <strong>of</strong> 12 months for each side (range, 12.0-<br />
61.5). The interval between replacements averaged 7.4 months (range, 0.5-26.0). Results: The mean unadjusted baseline<br />
Constant score for the first versus the second side was not significant (35 vs 41, P = .3). These scores improved to 73 and 72<br />
by final follow-up (both P < .0001). Mean pain scores on the visual analog scale (VAS) improved from 6.9 to 0.9 (P < .0001).<br />
We found no difference in the estimated blood loss (EBL), operative time, or hospital length <strong>of</strong> stay (LOS) between the sides.<br />
Significantly higher mean scores were demonstrated in all components <strong>of</strong> the SF-36 questionnaire over a normalized cohort<br />
<strong>of</strong> U.S. age-matched males and females by final follow-up. All patients were satisfied with both procedures. Conclusion:<br />
Staged, bilateral total shoulder arthroplasty results in excellent functional outcomes and high satisfaction in subjective<br />
patient assessment. We currently recommend a minimum <strong>of</strong> 6 weeks between replacements to allow for appropriate tissue<br />
healing and rehabilitation. Level <strong>of</strong> Evidence: 4. © <strong>2010</strong> Journal <strong>of</strong> Shoulder and Elbow Surgery Board <strong>of</strong> Trustees.<br />
No.356<br />
Author (s) : Guzman, M.G., Jaenisch, T., Gaczkowski, R., Hang, V.T.T., Sekaran, S.D., Kroeger, A., Vazquez, S., Ruiz, D., Martinez, E., Mercado,<br />
J.C., Balmaseda, A., Harris, E., Dimano, E., Leano, P.S.A., Yoksan, S., Villegas, E., Benduzu, H., Villalobos, I., Farrar, J., Simmons,<br />
C.P.<br />
Title : Multi-country evaluation <strong>of</strong> the sensitivity and specificity <strong>of</strong> two commercially-available NS1 ELISA assays for dengue diagnosis<br />
Source : PLoS Neglected Tropical Diseases, 4 (8), art. no. e811, .<br />
Document Type : Article<br />
Keywords : immunoglobulin M, nonstructural protein 1; antibody detection, article, Asia, blood donor, clinical trial, controlled clinical<br />
trial, controlled study, dengue, diagnostic accuracy, disease severity, early diagnosis, enzyme linked immunosorbent<br />
assay, fever, human, immunoassay, intermethod comparison, laboratory test, major clinical study, multicenter<br />
study, nonhuman, sensitivity and specificity, Western Hemisphere; Adolescent, Adult, Aged, Antibodies, Viral, Antigens,<br />
Viral, Child, Child, Preschool, Dengue, Enzyme-Linked Immunosorbent Assay, Humans, Immunoglobulin M, Infant, Middle<br />
Aged, Reagent Kits, Diagnostic, Sensitivity and Specificity, Viral Nonstructural Proteins, Virology, Young Adult<br />
<strong>Abstract</strong> : Background:Early diagnosis <strong>of</strong> dengue can assist patient triage and management and prevent unnecessary treatments and<br />
interventions. Commercially available assays that detect the dengue virus protein NS1 in the plasma/serum <strong>of</strong> patients <strong>of</strong>fers<br />
the possibility <strong>of</strong> early and rapid diagnosis. Methodology/Principal Findings:The sensitivity and specificity <strong>of</strong> the Pan-E Dengue<br />
Early ELISA and the PlateliaTM Dengue NS1 Ag assays were compared against a reference diagnosis in 1385 patients in 6<br />
countries in Asia and the Americas. Platelia was more sensitive (66%) than Pan-E (52%) in confirmed dengue cases. Sensitivity<br />
varied by geographic region, with both assays generally being more sensitive in patients from SE Asia than the Americas. Both<br />
kits were more sensitive for specimens collected within the first few days <strong>of</strong> illness onset relative to later time points. Pan-E<br />
129
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
and Platelia were both 100% specific in febrile patients without evidence <strong>of</strong> acute dengue. In patients with other confirmed<br />
diagnoses and healthy blood donors, Platelia was more specific (100%) than Pan-E (90%). For Platelia, when either the NS1<br />
test or the IgM test on the acute sample was positive, the sensitivity versus the reference result was 82% in samples<br />
collected in the first four days <strong>of</strong> fever. NS1 sensitivity was not associated to disease severity (DF or DHF) in the Platelia test,<br />
whereas a trend for higher sensitivity in DHF cases was seen in the Pan-E test (however combined with lower overall<br />
sensitivity). Conclusions/Significance:Collectively, this multi-country study suggests that the best performing NS1 assay<br />
(Platelia) had moderate sensitivity (median 64%, range 34-76%) and high specificity (100%) for the diagnosis <strong>of</strong> dengue. The<br />
poor sensitivity <strong>of</strong> the evaluated assays in some geographical regions suggests further assessments are needed. The<br />
combination <strong>of</strong> NS1 and IgM detection in samples collected in the first few days <strong>of</strong> fever increased the overall dengue<br />
diagnostic sensitivity. © <strong>2010</strong> Guzman et al.<br />
No.357<br />
Author (s) : Guzman, Maria G.; Halstead, Scott B.; Artsob, Harvey; Buchy, Philippe; Jeremy Farrar; Gubler, Duane J.; Hunsperger, Elizabeth;<br />
Kroeger, Axel; Margolis, Harold S.; Martinez, Eric; Nathan, Michael B.; Luis Pelegrino, Jose; Cameron Simmons; Yoksan, Sutee;<br />
Peeling, Rosanna W.<br />
Title : Dengue: a continuing global threat<br />
Source : NATURE REVIEWS MICROBIOLOGY, Supplement: S, Pages: S7-S16, DOI: 10.1038/nrmicro2460, Published: DEC <strong>2010</strong><br />
Document Type : Review<br />
Keywords : PLAQUE-REDUCTION NEUTRALIZATION; LINKED-IMMUNOSORBENT-ASSAY; NONSTRUCTURAL PROTEIN NS1; POLYMERASE CHAIN-<br />
REACTION; REVERSE-TRANSCRIPTASE PCR; TYPE-4 VACCINE CANDIDATE; HEALTHY ADULT VOLUNTEERS; ORIGINAL ANTIGENIC<br />
SIN; IMMUNOGLOBULIN-M IGM; T-CELL RESPONSES<br />
<strong>Abstract</strong> : Dengue fever and dengue haemorrhagic fever are important arthropod-brone viral diseases. Each year, there are similar to 50<br />
million dengue infections and similar to 500,000 individuals are hospitalized with dengue haemorrhagic fever, mainly in<br />
Southeast Asia, the Pacific and the Americas. Illness is produced by any <strong>of</strong> the four dengue virus serotypes. A global strategy<br />
aimed at increasing the capacity for surveillance and outbreak response, changing behaviours and reducing the disease<br />
burden using integrated vector management in conjunction with early and accurate diagnosis has been advocated. Antiviral<br />
drugs and vaccines that are currently under development could also make an important contribution to dengue control in<br />
the future.<br />
No.358<br />
Author (s) : Gwee, K.-A., Bak, Y.-T., Ghoshal, U.C., Gonlachanvit, S., Lee, O.Y., Fock, K.M., Chua, A.S.B., Lu, C.-L., Goh, K.-L., Kositchaiwat, C.,<br />
Makharia, G., Park, H.-J., Chang, F.-Y., Fukudo, S., Choi, M.-G., Bhatia, S., Ke, M., Hou, X., Hongo, M.<br />
Title : Asian consensus on irritable bowel syndrome<br />
Source : Journal <strong>of</strong> Gastroenterology and Hepatology, Volume 25, Issue 7, July <strong>2010</strong>, Pages 1189-1205<br />
Document Type : Article<br />
Keywords : Asia; diagnosis; diet; epidemiology; irritable bowel syndrome; pathophysiology; symptom<br />
<strong>Abstract</strong> : Background and Aims: Many <strong>of</strong> the ideas on irritable bowel syndrome (IBS) are derived from studies conducted in Western<br />
societies. Their relevance to Asian societies has not been critically examined. Our objectives were to bring to attention<br />
important data from Asian studies, articulate the experience and views <strong>of</strong> our Asian experts, and provide a relevant guide on<br />
this poorly understood condition for doctors and scientists working in Asia. Methods: A multinational group <strong>of</strong> physicians from<br />
Asia with special interest in IBS raised statements on IBS pertaining to symptoms, diagnosis, epidemiology, infection,<br />
pathophysiology, motility, management, and diet. A modified Delphi approach was employed to present and grade the<br />
quality <strong>of</strong> evidence, and determine the level <strong>of</strong> agreement. Results: We observed that bloating and symptoms associated<br />
with meals were prominent complaints among our IBS patients. In the majority <strong>of</strong> our countries, we did not observe a female<br />
predominance. In some Asian populations, the intestinal transit times in healthy and IBS patients appear to be faster than<br />
those reported in the West. High consultation rates were observed, particularly in the more affluent countries. There was only<br />
weak evidence to support the perception that psychological distress determines health-care seeking. Dietary factors, in<br />
particular, chili consumption and the high prevalence <strong>of</strong> lactose malabsorption, were perceived to be aggravating factors, but<br />
the evidence was weak. Conclusions: This detailed compilation <strong>of</strong> studies from different parts <strong>of</strong> Asia, draws attention to<br />
Asian patients' experiences <strong>of</strong> IBS. © <strong>2010</strong> Journal <strong>of</strong> Gastroenterology and Hepatology Foundation and Blackwell Publishing<br />
Asia Pty Ltd.<br />
130
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.359<br />
Author (s) : Haddow, L.J., Colebunders, R., Meintjes, G., Lawn, S.D., Elliott, J.H., Manabe, Y.C., Bohjanen, P.R., Sungkanuparph, S.,<br />
Easterbrook, P.J., French, M.A., Boulware, D.R.<br />
Title : Cryptococcal immune reconstitution inflammatory syndrome in HIV-1-infected individuals: proposed clinical case definitions<br />
Source : The Lancet Infectious Diseases, Volume 10, Issue 11, November <strong>2010</strong>, Pages 791-802<br />
Document Type : Review<br />
Keywords : corticosteroid, fluconazole, nonsteroid antiinflammatory agent, prednisone, thalidomide; antigen detection, Cryptococcal<br />
immune reconstitution inflammatory syndrome, cryptococcal meningitis, cryptococcosis, Cryptococcus, Cryptococcus<br />
ne<strong>of</strong>ormans, epidemiology, fungus culture, highly active antiretroviral therapy, human, Human immunodeficiency virus<br />
1, Human immunodeficiency virus 1 infection, Human immunodeficiency virus infected patient, immune reconstitution<br />
inflammatory syndrome, immunopathology, lymphadenopathy, pneumonia, priority journal, review, skin<br />
defect, Strongyloides, tuberculosis; Anti-HIV Agents, Cryptococcosis, Cryptococcus, HIV Infections, HIV-1, Humans, Immune<br />
Reconstitution Inflammatory Syndrome<br />
<strong>Abstract</strong> : Cryptococcal immune reconstitution inflammatory syndrome (IRIS) may present as a clinical worsening or new presentation <strong>of</strong><br />
cryptococcal disease after initiation <strong>of</strong> antiretroviral therapy (ART), and is thought to be caused by recovery <strong>of</strong> cryptococcusspecific<br />
immune responses. We have reviewed reports <strong>of</strong> cryptococcal IRIS and have developed a consensus case definition<br />
specifically for paradoxical crytopcoccal IRIS in patients with HIV-1 and known cryptococcal disease before ART, and a<br />
separate definition for incident cryptococcosis developed during ART (termed ART-associated cryptococcosis), for which a<br />
proportion <strong>of</strong> cases are likely to be unmasking cryptococcal IRIS. These structured case definitions are intended to aid design<br />
<strong>of</strong> future clinical, epidemiological, and immunopathological studies <strong>of</strong> cryptococcal IRIS, to standardise diagnostic criteria, and<br />
to facilitate comparisons between studies. As for definitions <strong>of</strong> tuberculosis-associated IRIS, definitions for cryptococcal IRIS<br />
should be regarded as preliminary until further insights into the immunopathology <strong>of</strong> IRIS permit their refinement. © <strong>2010</strong><br />
Elsevier Ltd.<br />
No.360<br />
Author (s) : Haginoya, K., Thangavel, V., Pandian, G.N., Tomimoto, K., Shitomi, Y., Azuma, M., Angsuthanasombat, C., Hori, H.<br />
Title : Investigation <strong>of</strong> physicochemical condition to stabilize phosphatidylcholineliposome enclosing fluorescent calcein and its<br />
exploitation for analysis <strong>of</strong> pore formation with Cry1A toxins <strong>of</strong> Bacillus thuringiensis<br />
Source : Applied Entomology and Zoology, Volume 45, Issue 3, <strong>2010</strong>, Pages 477-488<br />
Document Type : Article<br />
Keywords : Bacillus thuringiensis; Calcein release assay; PC liposome; Pore formation with Cry1A toxins<br />
<strong>Abstract</strong> : Liposome was prepared using phosphatidylcholine (PC), and calcein, a fluorescent chemical, was simultaneously enclosed<br />
within the liposome (PC-Lipo). The stability <strong>of</strong> PC-Lipo in its ability to retain calcein was evaluated under various conditions.<br />
PC-Lipo lost stability at pH 6 and pH 11-13, but was stable in the range <strong>of</strong> pH 8.3-10. PC-Lipo was stable in the temperature<br />
range <strong>of</strong> 15-30°C, but lost the stability acutely at 35°C. Ionic strength, given as the concentration <strong>of</strong> NaCl, also affects its<br />
stability, and a higher concentration <strong>of</strong> NaCl, i.e., more than 150mM, induced a higher leak <strong>of</strong> calcein from PC-Lipo. The<br />
optimal conditions to achieve stable PC-Lipo were employed to characterize the differences in pore formation with Bacillus<br />
thuringiensis Cry1Aa, Cry1Ab and Cry1Ac toxins. These toxins were reacted with PC-Lipo under these optimal conditions, and<br />
the affinity and maximum speed <strong>of</strong> Cry1Ab to form pores on PC-Lipo was shown to be highest. Here we show these<br />
conditions and evidence <strong>of</strong> the usefulness <strong>of</strong> PC-Lipo to investigate the mode <strong>of</strong> action <strong>of</strong> Cry1A toxins.<br />
No.361<br />
Author (s) : Hahnvajanawong, C., Boonyanugomol, W., Nasomyon, T., Loilome, W., Namwat, N., Anantachoke, N., Tassaneeyakul, W., Sripa,<br />
B., Namwat, W., Reutrakul, V.<br />
Title : Apoptotic activity <strong>of</strong> caged xanthones from Garcinia hanburyi in cholangiocarcinoma cell lines<br />
Source : World Journal <strong>of</strong> Gastroenterology, Volume 16, Issue 18, 14 May <strong>2010</strong>, Pages 2235-2243<br />
Document Type : Article<br />
Keywords : Apoptosis; Caged xanthones; Garcinia hanburyi; Human cholangiocarcinoma cell lines<br />
<strong>Abstract</strong> : AIM: To investigate the growth inhibitory mechanism <strong>of</strong> four caged xanthones from Garcinia hanburyi in cholangiocarcinoma<br />
(CCA) KKU-100 and KKU-M156 cells. METHODS: Four caged xanthones, selected on the basis <strong>of</strong> their anticancer potency and<br />
chemical structure diversities (i.e. isomorellin, isomorellinol, forbesione and gambogic acid) were used in this study. Growth<br />
inhibition <strong>of</strong> these caged xanthones was determined using the sulforhodamine B assay. Induction <strong>of</strong> apoptosis was assessed<br />
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by observing cell morphology, ethidium bromide and acridine orange staining and DNA fragmentation assay. Levels <strong>of</strong><br />
apoptotic-related gene and protein expressions were determined by a real-time reverse transcriptase polymerase chain<br />
reaction and Western blotting analysis, respectively. RESULTS: The compounds were found to inhibit growth <strong>of</strong> both cell lines<br />
in a dose-dependent manner and also showed selective cytotoxicity against the cancer cells when compared with normal<br />
peripheral blood mononuclear cells. Growth suppression by these compounds was due to apoptosis, as evidenced by the<br />
cell morphological changes, chromatin condensation, nuclear fragmentation, and DNA ladder formation. At the molecular<br />
level, these compounds induced down-regulation <strong>of</strong> Bcl-2 and survivin proteins with up-regulation <strong>of</strong> Bax and<br />
apoptosisinducing factor proteins, leading to the activation <strong>of</strong> caspase-9 and -3 and DNA fragmentation. The functional group<br />
variations did not appear to affect the anticancer activity with regard to the two CCA cell lines; however, at a mechanistic<br />
level, isomorellinol exhibited the highest potency in increasing the Bax/Bcl-2 protein expression ratio (120 and 41.4 for KKU-<br />
100 and KKU-M156, respectively) and in decreasing survivin protein expression (0.01 fold as compared to control cells in both<br />
cell lines). Other activities at the molecular level indicate that functional groups on the prenyl side chain may be important.<br />
CONCLUSION: Our findings for the first time demonstrate that four caged xanthones induce apoptosis in CCA cells which is<br />
mediated through a mitochondriadependent signaling pathway. © <strong>2010</strong> Baishideng.<br />
No.362<br />
Author (s) : Halstead, S.B., Mahalingam, S., Marovich, M.A., Ubol, S., Mosser, D.M.<br />
Title : Intrinsic antibody-dependent enhancement <strong>of</strong> microbial infection in macrophages: Disease regulation by immune complexes<br />
Source : The Lancet Infectious Diseases, Volume 10, Issue 10, October <strong>2010</strong>, Pages 712-722<br />
Document Type : Review<br />
Keywords : Fc receptor; antibody dependent enhancement, dengue, Dengue virus, disease severity, human, immune complex<br />
disease, infection, leishmaniasis, macrophage, nonhuman, pathogenesis, priority journal, review, Ross River alpha virus;<br />
Animals, Antibody-Dependent Enhancement, Antigen-Antibody Complex, Bacteria, Eukaryota, Humans, Immunoglobulin<br />
G, Macrophages, Monocytes, Receptors, IgG, Th1 Cells, Th2 Cells, Viruses<br />
<strong>Abstract</strong> : A wide range <strong>of</strong> microorganisms can replicate in macrophages, and cell entry <strong>of</strong> these pathogens via non-neutralising IgG<br />
antibody complexes can result in increased intracellular infection through idiosyncratic Fcγ-receptor signalling. The activation<br />
<strong>of</strong> Fcγ receptors usually leads to phagocytosis. Paradoxically, the ligation <strong>of</strong> monocyte or macrophage Fcγ receptors by IgG<br />
immune complexes, rather than aiding host defences, can suppress innate immunity, increase production <strong>of</strong> interleukin 10,<br />
and bias T-helper-1 (Th1) responses to Th2 responses, leading to increased infectious output by infected cells. This intrinsic<br />
antibody-dependent enhancement (ADE) <strong>of</strong> infection modulates the severity <strong>of</strong> diseases as disparate as dengue haemorrhagic<br />
fever and leishmaniasis. Intrinsic ADE is distinct from extrinsic ADE, whereby complexes <strong>of</strong> infectious agents with nonneutralising<br />
antibodies lead to an increased number <strong>of</strong> infected cells. Intrinsic ADE might be involved in many protozoan,<br />
bacterial, and viral infections. We review insights into intracellular mechanisms and implications <strong>of</strong> enhanced pathogenesis<br />
after ligation <strong>of</strong> macrophage Fcγ receptors by infectious immune complexes. © <strong>2010</strong> Elsevier Ltd.<br />
No.363<br />
Author (s) : Hamann, C.R., Hamann, D.J., Hamann, Q.J., Hamann, C.P., Boonchai, W., Li, L.-F., Thyssen, J.P.<br />
Title : Assessment <strong>of</strong> nickel release from earrings randomly purchased in China and Thailand using the dimethylglyoxime test<br />
Source : Contact Dermatitis, Volume 62, Issue 4, April <strong>2010</strong>, Pages 232-240<br />
Document Type : Article<br />
Keywords : Dimethylglyoxime test; Earrings; Nickel allergy; Nickel release; Prevalence; Risk exposure; XRF spectrometry<br />
<strong>Abstract</strong> : Background: China and Thailand produce large amounts <strong>of</strong> jewellery that are sold domestically and abroad. Objective: To<br />
identify nickel release and metal content in earrings purchased in China and Thailand. Methods: A total <strong>of</strong> 557 earrings were<br />
randomly purchased from vendors in 11 markets located in Beijing, Chengdu, Bangkok, Patong Beach, and Hat Yai. Earrings<br />
were subjected to dimethylglyoxime (DMG) tests and 26 <strong>of</strong> the DMG negative earrings were further evaluated qualitatively for<br />
major and minor metal content using X-ray fluorescence spectrometry. Results: A total <strong>of</strong> 314 Chinese earrings (31.5%) and<br />
243 Thai earrings (29.2%) were DMG test positive. Three (11.5%) <strong>of</strong> 29 DMG negative earrings contained nickel as a major<br />
component; 7 (26.9%) <strong>of</strong> 29 DMG negative earrings contained nickel as a minor component. Discussion: Excessive nickel<br />
release was frequent. This may contribute to the high prevalence <strong>of</strong> nickel allergy in both countries. Increased public<br />
education together with the adoption <strong>of</strong> a regulatory intervention in Thailand may be warranted. In China, enforcement <strong>of</strong><br />
the 2002 Chinese National Standard GB 11887 may assist in reducing sensitization. These initiatives may eventually result in<br />
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decreased morbidity among the Chinese and Thai citizens, but can potentially benefit recipient countries <strong>of</strong> these important<br />
earring producing countries. © <strong>2010</strong> John Wiley & Sons A/S.<br />
No.364<br />
Author (s) : Hamzah, Z., Petmitr, S., Mungthin, M., Leelayoova, S., Chavalitshewinkoon-Petmitr, P.<br />
Title : Development <strong>of</strong> multiplex real-time polymerase chain reaction for detection <strong>of</strong> Entamoeba histolytica, Entamoeba dispar,<br />
and Entamoeba moshkovskii in clinical specimens<br />
Source : American Journal <strong>of</strong> Tropical Medicine and Hygiene, Volume 83, Issue 4, October <strong>2010</strong>, Pages 909-913<br />
Document Type : Article<br />
Keywords : DNA, protozoal DNA; amebiasis, article, clinical article, controlled study, DNA determination, Entamoeba dispar, Entamoeba<br />
histolytica, entamoeba moshkovskii, high resolution melting analysis, human, hybridization, microscopy, molecular<br />
probe, multiplex real time polymerase chain reaction, nonhuman, nucleotide sequence, real time polymerase chain<br />
reaction, sensitivity analysis, sensitivity and specificity, Thailand, classification, Entamoeba, feces, genetics, isolation and<br />
purification, methodology, parasitology, polymerase chain reaction, species difference, time; DNA,<br />
Protozoan, Entamoeba, Entamoebiasis, Feces, Humans, Polymerase Chain Reaction, Sensitivity and Specificity, Species<br />
Specificity, Thailand, Time Factors<br />
<strong>Abstract</strong> : Multiplex real-time polymerase chain reaction (PCR) was developed for differential detection <strong>of</strong> Entamoeba histolytica,<br />
Entamoeba dispar, and Entamoeba moshkovskii. Specific primers were designed for all three species, and then differentiation<br />
<strong>of</strong> E. histolytica and E. dispar was achieved simultaneously using a hybridization probe and melting curve analysis, whereas E.<br />
moshkovskii was detected with a separate probe under the same condition. This assay detected as little as 0.2 pg <strong>of</strong> E.<br />
histolytica DNA and 2 pg each for E. dispar and E. moshkovskii DNA. Thirty-five clinical samples suspected to be E. histolytica<br />
infection by microscopy were tested. The results showed 32 positive samples; four samples were E. histolytica and 28<br />
samples were E. dispar. Interestingly, one E. dispar positive sample showed a mixed infection with E. moshkovskii. This is the<br />
first report <strong>of</strong> E. moshkovskii infection from Thailand and this assay is currently the most rapid and sensitive method to<br />
differentiate these human amoebas. Copyright © <strong>2010</strong> by The American Society <strong>of</strong> Tropical Medicine and Hygiene.<br />
No.365<br />
Author (s) : Hanboon, B.K., Ekataksin, W., Alsfasser, G., Schemmer, P., Urbaschek, B., McCuskey, R.S., Klar, E.<br />
Title : Microvascular dysfunction in hepatic ischemia-reperfusion injury in pigs<br />
Source : Microvascular Research, Volume 80, Issue 1, July <strong>2010</strong>, Pages 123-132<br />
Document Type : Article<br />
Keywords : Hepatic artery dilation; Hepatocyte swelling; Portal edema; Portal vein constriction; Sinusoidal collapse<br />
<strong>Abstract</strong> : Although hepatic ischemia-reperfusion (I/R) injury has been investigated for more than two decades, histopathological<br />
documentation is limited. As a result, three pig livers with I/R injury and three control livers were injected with colored media,<br />
cut into 14 segments, and examined by light microscopy together with microscopic map making. In livers with I/R injury,<br />
lobules were identified as being occluded or unoccluded. The proportion <strong>of</strong> the occluded lobules increased in a<br />
caudocephalic fashion, while that <strong>of</strong> the unoccluded lobules decreased (X2 for linear trend, P<0.0001). Especially in the<br />
occluded lobules, swollen hepatic plates displayed various forms <strong>of</strong> cellular distortion. Collapsed sinusoids containing<br />
leukocyte aggregation and shrunken central veins were observed together with reduced caliber <strong>of</strong> the contiguous sublobular<br />
veins. Portal vein constriction with loosening <strong>of</strong> the surrounding stroma suggestive <strong>of</strong> edema and hepatic artery dilation were<br />
also seen. Isolated arterioles and transintimal vasal outlets <strong>of</strong> the hepatic vein's vasa venarum were dilated and frequently<br />
observed. In conclusion, I/R injury affected the liver parenchyma, the microvasculature, and its surrounding stroma. The<br />
heterogeneous distribution <strong>of</strong> occluded and unoccluded lobules is suggested due to the difference <strong>of</strong> vascular structure in<br />
various liver segments. The constrictive/obstructive changes in the portosinusoidal-hepatic vascular pr<strong>of</strong>ile suggest a definite<br />
increase in resistance at presinusoidal, sinusoidal, and proximal postsinusoidal levels, resulting in an expansion <strong>of</strong> the arterial<br />
shunt circulation. © <strong>2010</strong> Elsevier Inc.<br />
No.366<br />
Author (s) : Hanson, J., Lee, S.J., Mohanty, S., Faiz, M.A., Anstey, N.M., Charunwatthana, P., Yunus, E.B., Mishra, S.K., Tjitra, E., Price, R.N.,<br />
Rahman, R., Nosten, F., Htut, Y., Hoque, G., Chau, T.T.H., Phu, N.H., Hien, T.T., White, N.J., Day, N.P.J., Dondorp, A.M.<br />
Title : A simple score to predict the outcome <strong>of</strong> severe malaria in adults<br />
Source : Clinical Infectious Diseases, Volume 50, Issue 5, 1 March <strong>2010</strong>, Pages 679-685<br />
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Document Type : Article<br />
Keywords : bicarbonate; acidosis, adult, article, Bangladesh, bicarbonate blood level, brain malaria, coma acidosis malaria score, Glasgow<br />
coma scale, human, kidney failure, major clinical study, malaria, mortality, outcome assessment, prediction, predictor<br />
variable, priority journal, scoring system, Southeast Asia, Viet Nam;<br />
Acidosis, Adult, Bangladesh, Coma, Female, Humans, Malaria, Male, Prognosis, Severity <strong>of</strong> Illness Index, Vietnam<br />
<strong>Abstract</strong> : Background. World Health Organization treatment guidelines recommend that adults with severe malaria be admitted to an<br />
intensive care unit (ICU). However, ICU facilities are limited in the resource-poor settings where most malaria occurs.<br />
Identification <strong>of</strong> patients at greater risk <strong>of</strong> complications may facilitate their triage and resource allocation. Methods. With use<br />
<strong>of</strong> data from a trial conducted in Southeast Asia (n = 868), a logistic regression model was built to identify independent<br />
predictors <strong>of</strong> mortality among adults with severe malaria. A scoring system based on this model was tested in the original<br />
dataset and then validated in 2 series from Bangladesh (n = 188) and Vietnam (n = 292). Results. Acidosis (base deficit) and<br />
cerebral malaria (measured as Glasgow Coma Score) were the main independent predictors <strong>of</strong> outcome. The 5-point Coma<br />
Acidosis Malaria (CAM) score was simply derived from these 2 variables. Mortality increased steadily with increasing score. A<br />
CAM score
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
malaria programmes with the general health system, the integration <strong>of</strong> the Global Fund-portfolios within the national disease<br />
programmes, and system-wide effects on the health system in Thailand. The study relied on a literature review and 34<br />
interviews with key stakeholders using the Systemic Rapid Assessment Toolkit and thematic analysis. In Thailand, the HIV, TB<br />
and malaria programmes' structures and functions are well established in the general health care system, with the<br />
Department for Disease Control and the Ministry <strong>of</strong> Public Health's network <strong>of</strong> health providers at sub-national levels as the<br />
main responsible organizations for stewardship and governance, service delivery, monitoring and evaluation, planning, and to<br />
some extent, demand generation. Civil society groups are active in certain areas, particularly in demand generation for<br />
HIV/AIDS. Overall, the Global Fund-supported programmes were almost fully integrated and coordinated with the general<br />
health system. The extent <strong>of</strong> integration varied across disease portfolios because <strong>of</strong> different number <strong>of</strong> actors and the nature<br />
<strong>of</strong> programme activities. There were also specific requirements by Global Fund that limit integration for some health system<br />
functions namely financing and monitoring and evaluation. From the view <strong>of</strong> stakeholders in Thailand, the Global Fund has<br />
contributed significantly to the three diseases, particularly HIV/AIDS. Financial support from the early Global Fund rounds was<br />
particularly helpful to the disease programmes during the time <strong>of</strong> major structural change in the MoPH. It also promoted<br />
collaborative networks <strong>of</strong> stakeholders, especially civil societies. However, the impacts on the overall health system, which is<br />
relatively well developed, are seen as minimal. One major contribution is the establishment <strong>of</strong> a health service system for<br />
neglected population groups. No specific negative impacts on the health system were raised.<br />
No.369<br />
Author (s) : Harnirattisai, C., Luangaram, C., Kuphasuk, W., Senawongse, P.<br />
Title : The influence <strong>of</strong> a local anesthetic containing vasoconstrictor on microtensile bond strengths <strong>of</strong> two adhesive systems to<br />
human dentin in situ.<br />
Source : The journal <strong>of</strong> adhesive dentistry, Volume 12, Issue 1, February <strong>2010</strong>, Pages 11-18<br />
Document Type : Article<br />
Keywords : bisphenol A bis(2 hydroxypropyl) ether dimethacrylate, Clearfil SE Bond, dentin bonding agent, Filtek Z250, local anesthetic<br />
agent, resin, resin cement, single bond, vasoconstrictor agent; analysis <strong>of</strong> variance, article, chemistry, comparative<br />
study, dental acid etching, dental bonding, dental care, dentin, human, materials testing, methodology, tensile strength; Acid<br />
Etching, Dental, Analysis <strong>of</strong> Variance, Anesthetics, Local, Bisphenol A-Glycidyl Methacrylate, Composite Resins, Dental<br />
Bonding, Dental Stress Analysis, Dentin-Bonding Agents, Dentinal Fluid, Humans, Materials Testing, Resin Cements, Tensile<br />
Strength, Vasoconstrictor Agents<br />
<strong>Abstract</strong> : PURPOSE: To evaluate the bond strengths <strong>of</strong> an etch-and-rinse adhesive and a self-etching adhesive to human dentin in vitro<br />
and under two in situ conditions: non-anesthetization and anesthetization <strong>of</strong> the teeth with a local anesthetic containing<br />
vasoconstrictor. MATERIALS AND METHODS: An in situ study was performed on 49 maxillary premolars scheduled for<br />
extraction due to orthodontic reasons. For the bond strength test, occlusal cavities were prepared either with or without a<br />
local anesthetic containing vasoconstrictor. The cavities were bonded with Adper Single Bond or Clearfil SE Bond and filled<br />
with a resin composite, Filtek Z250. The teeth were then extracted, sectioned, trimmed, and tested for microtensile bond<br />
strength. For the observation <strong>of</strong> dentin surfaces, 20 premolars were used. The impressions <strong>of</strong> the cavity floors in the<br />
anesthetized and non-anesthetized groups were taken before and after acid etching. The replicas were observed under a<br />
scanning electron microscope. In vitro, the bond strength test and the SEM observation were also carried out on 24 extracted<br />
premolars with the same procedures used in the in situ study. RESULTS: The bond strengths <strong>of</strong> the two adhesives bonded to<br />
dentin in situ were significantly lower than those in vitro. When both adhesives were tested under in situ conditions, there<br />
were no significant differences between the bond strengths to dentin <strong>of</strong> anesthetized and non-anesthetized groups (p > 0.05).<br />
No fluid droplets were found on dentin on the cavity floor prepared in vitro, either before or after acid-etching. For the<br />
unetched dentin prepared in situ, fluid droplets were found and covered on the smear layer in anesthetized and nonanesthetized<br />
groups, but the droplets were slightly larger in the non-anesthetized group. In the non-anesthetized, acid-etched<br />
group, dentin surfaces were covered with coalescent dentinal fluid in most specimens. However, in the anesthetized, acidetched<br />
group, patent tubules and some dentinal fluid were observed on the surfaces. CONCLUSION: It was concluded that in<br />
situ, dentinal fluid had a detrimental effect on the dentin bond strengths <strong>of</strong> an etch-and-rinse adhesive and a self-etching<br />
adhesive. However, reduction in dentinal fluid due to the effect <strong>of</strong> a local anesthetic containing vasoconstrictor did not<br />
improve the bond strengths in either adhesives.<br />
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No.370<br />
Author (s) : Harris, S.R., Feil, E.J., Holden, M.T.G., Quail, M.A., Nickerson, E.K., Chantratita, N., Gardete, S., Tavares, A., Day, N., Lindsay, J.A.,<br />
Edgeworth, J.D., De Lencastre, H., Parkhill, J., Peacock, S.J., Bentley, S.D.<br />
Title : Evolution <strong>of</strong> MRSA during hospital transmission and intercontinental spread<br />
Source : Science, Volume 327, Issue 5964, 22 January <strong>2010</strong>, Pages 469-474<br />
Document Type : Article<br />
Keywords : bacterial DNA, fusidic acid, genomic DNA, pseudomonic acid, quinoline derived antiinfective agent, rifampicin, trimethoprim;<br />
epidemiology, genomics, hospital sector, infectious disease, microbial ecology, pathotype; article, bacterial infection, bacterial<br />
transmission, ecology, evolution, gene mapping, gene sequence, genomics, geography, hospital, methicillin resistant<br />
Staphylococcus aureus, nonhuman, priority journal, single nucleotide polymorphism, Asia, bacterial genome, bacterium<br />
identification, classification, cross infection, disease transmission, DNA sequence, Europe, genetics, human, isolation and<br />
purification, methodology, microbiology, molecular epidemiology, molecular evolution, molecular genetics, phylogeny, South<br />
America, Staphylococcus infection, statistical model, time, United States; Bacteria (microorganisms), methicillin resistant<br />
Staphylococcus aureus; Asia, Bacterial Typing Techniques, Cross Infection, Europe, Evolution, Molecular, Genome,<br />
Bacterial, Genomics, Humans, Likelihood Functions, Methicillin-Resistant Staphylococcus aureus, Molecular<br />
Epidemiology, Molecular Sequence Data, Phylogeny, Polymorphism, Single Nucleotide, Sequence Analysis, DNA, South<br />
America, Staphylococcal Infections, Time Factors, United States<br />
<strong>Abstract</strong> : Current methods for differentiating isolates <strong>of</strong> predominant lineages <strong>of</strong> pathogenic bacteria <strong>of</strong>ten do not provide sufficient<br />
resolution to define precise relationships. Here, we describe a highthroughput genomics approach that provides a highresolution<br />
view <strong>of</strong> the epidemiology and microevolution <strong>of</strong> a dominant strain <strong>of</strong> methicillin-resistant Staphylococcus aureus<br />
(MRSA). This approach reveals the global geographic structure within the lineage, its intercontinental transmission through four<br />
decades, and the potential to trace person-to-person transmission within a hospital environment. The ability to interrogate<br />
and resolve bacterial populations is applicable to a range <strong>of</strong> infectious diseases, as well as microbial ecology.<br />
No.371<br />
Author (s) : Haseen, F., Adhikari, R., Soonthorndhada, K.<br />
Title : Self-assessed health among Thai elderly<br />
Source : BMC Geriatrics, Volume 10, <strong>2010</strong>, Article number 30<br />
Document Type : Article<br />
Keywords : age, aged, article, comparative study, female, health status, health survey, human, male, middle aged, randomization, self<br />
evaluation, socioeconomics, Thailand; Age Factors, Aged, Aged, 80 and over, Female, Health Status, Health<br />
Surveys, Humans, Male, Middle Aged, Random Allocation, Self-Assessment, Socioeconomic Factors, Thailand<br />
<strong>Abstract</strong> : Background. The ageing <strong>of</strong> the population is rapidly progressing in Thailand. Self-assessed health status can provide a holistic<br />
view <strong>of</strong> the health <strong>of</strong> the elderly. This study aims to identify the determinants <strong>of</strong> self-assessed health among older Thai<br />
people. Methods. The data for this study were drawn from a national survey <strong>of</strong> older persons conducted in 2007. Stratified<br />
two-stage random sampling was used for data collection. The analysis was restricted to the population aged 60 and above.<br />
The study used univariate, bivariate, and multivariate analysis procedures to analyze the data. Bivariate analysis was used to<br />
identify the factors associated with self assessment <strong>of</strong> health status. After controlling for other variables, the variables were<br />
further examined using multivariate analysis (binary logistic regression) in order to identify the significant predictors <strong>of</strong> the<br />
likelihood <strong>of</strong> reporting poor health. Results. Overall, 30,427 elderly people were interviewed in this study. More than half <strong>of</strong><br />
the sampled respondents (53%) were aged 60-69 years and about one out <strong>of</strong> seven (13%) were aged 80 years or above.<br />
About three in five respondents (56%) reported that their health was either fair or very bad/bad. Logistic regression analysis<br />
found that age, education, marital status, working status, income, functional status, number <strong>of</strong> chronic diseases, and number<br />
<strong>of</strong> psychosocial symptoms are significant predictors in determining health status. Respondents who faced more difficulty in<br />
daily life were more likely to rate their health as poor compared to those who faced less such difficulty. For instance,<br />
respondents who could not perform 3 or more activities <strong>of</strong> daily living (ADLs) were 3.3 times more likely to assess their health<br />
as poor compared to those who could perform all the ADLs. Similarly, respondents who had 1, 2, or 3 or more chronic<br />
diseases were 1.8 times, 2.4 times, and 3.7 times, respectively, more likely to report their health as poor compared to those<br />
who had no chronic disease at all. Moreover, respondents who had 1-2, 3-4, or 5 or more psychosocial symptoms in the<br />
previous months were 1.6 times, 2.2 times, and 2.7 times, respectively, more likely to report poor health compared to those<br />
who did not have any psychosocial symptoms during the same period. Conclusion. Self-assessed poor health is not<br />
uncommon among older people in Thailand. No single factor accounts for the self-assessed poor health. The study has found<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
that chronic disease, functional status, and psychosocial symptoms are the strongest determinants <strong>of</strong> self-assessed poor<br />
health <strong>of</strong> elderly people living in Thailand. Therefore, health-related programs should focus on all the factors identified in<br />
this paper to improve the overall well-being <strong>of</strong> the ageing population <strong>of</strong> Thailand. © <strong>2010</strong> Haseen et al; licensee BioMed<br />
Central Ltd.<br />
No.372<br />
Author (s) : Hasthanasombut, S., Ntui, V., Supaibulwatana, K., Mii, M., Nakamura, I.<br />
Title : Expression <strong>of</strong> Indica rice OsBADH1 gene under salinity stress in transgenic tobacco<br />
Source : Plant Biotechnology Reports, Volume 4, Issue 1, January <strong>2010</strong>, Pages 75-83<br />
Document Type : Article<br />
Keywords : Agrobacterium tumefaciens; Betaine aldehyde dehydrogenase (BADH); Gene transformation; Glycine betaine; Osmotic<br />
stress; Salinity stress<br />
<strong>Abstract</strong> : Glycine betaine has been reported as an osmoprotectant compound conferring tolerance to salinity and osmotic stresses in<br />
plants. We previously found that the expression <strong>of</strong> betaine aldehyde dehydrogenase 1 gene (OsBADH1), encoding a key<br />
enzyme for glycine betaine biosynthesis pathway, showed close correlation with salt tolerance <strong>of</strong> rice. In this study, the<br />
expression <strong>of</strong> the OsBADH1 gene in transgenic tobacco was investigated in response to salt stress using a transgenic approach.<br />
Transgenic tobacco plants expressing the OsBADH1 gene were generated under the control <strong>of</strong> a promoter from the maize<br />
ubiquitin gene. Three homozygous lines <strong>of</strong> T2 progenies with single transgene insert were chosen for gene expression analysis.<br />
RT-PCR and western blot analysis results indicated that the OsBADH1 gene was effectively expressed in transgenic tobacco<br />
leading to the accumulation <strong>of</strong> glycine betaine. Transgenic lines demonstrated normal seed germination and morphology,<br />
and normal growth rates <strong>of</strong> seedlings under salt stress conditions. These results suggest that the OsBADH1 gene could be an<br />
excellent candidate for producing plants with osmotic stress tolerance. © Korean Society for Plant Biotechnology and Springer<br />
2009.<br />
No.373<br />
Author (s) : Hattori, M.K., Richter, K., Greene, J.<br />
Title : Trust, caution, and condom use with regular partners: An evaluation <strong>of</strong> the trusted partner campaign targeting youth in four<br />
countries<br />
Source : Social Marketing Quarterly, Volume 16, Issue 2, <strong>2010</strong>, Pages 18-48<br />
Document Type : Article<br />
Keywords : –<br />
<strong>Abstract</strong> : Research suggests that youth do not use condoms because they trust their partner. This article describes the Trusted Partner<br />
regional behavior change communication campaign and uses data from Lesotho, Mozambique, Uganda, and Zambia to<br />
evaluate the impact <strong>of</strong> the campaign. Cross-sectional surveys <strong>of</strong> youth aged 15 to 24 were conducted in each country before<br />
and after the campaign. The surveys measured elements related to trust and condom use with a regular partner. Multivariate<br />
logistic regressions were used to investigate the determinants <strong>of</strong> condom use and whether exposure to the campaign was<br />
associated with condom use. The evaluation <strong>of</strong> the campaign showed mixed results. The percent <strong>of</strong> youth recalling the<br />
campaign varied among countries: 32% in urban Lesotho, 54% in urban Mozambique, 16% in Uganda, and 39% in urban<br />
Zambia. In all 4 countries, sexual caution with a partner had a positive relationship with condom use. However, exposure to<br />
the campaign was only associated with condom use in Mozambique. These results from Mozambique indicate that more<br />
intense exposure may be necessary to achieve impact. Regional campaigns appear to be a cost-effective way to reach large<br />
numbers <strong>of</strong> youth, but more detailed measures <strong>of</strong> exposure should be pursued to improve program evaluations.<br />
No.374<br />
Author (s) : Helton, J.S., Matan, K., Shores, M.P., Nytko, E.A., Bartlett, B.M., Qiu, Y., Nocera, D.G., Lee, Y.S.<br />
Title : Dynamic scaling in the susceptibility <strong>of</strong> the spin-12 kagome lattice antiferromagnet herbertsmithite<br />
Source : Physical Review Letters, Volume 104, Issue 14, 6 April <strong>2010</strong>, Article number 147201<br />
Document Type : Article<br />
Keywords : AC susceptibility, Antiferromagnets, Candidate materials, Dynamic scaling, Kagome lattice, Low energies, Magnetic<br />
response, Quantum critical points, Quantum spin liquid, Scaling relations, Universal functions; Antiferromagnetic<br />
materials, Dynamic response, Equations <strong>of</strong> state, Magnetic susceptibility, Quantum theory; Spin dynamics<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : The spin-12 kagome lattice antiferromagnet herbertsmithite, ZnCu3(OH)6Cl2, is a candidate material for a quantum spin liquid<br />
ground state. We show that the magnetic response <strong>of</strong> this material displays an unusual scaling relation in both the bulk ac<br />
susceptibility and the low energy dynamic susceptibility as measured by inelastic neutron scattering. The quantity χTα with<br />
α0.66 can be expressed as a universal function <strong>of</strong> H/T or ω/T. This scaling is discussed in relation to similar behavior seen in<br />
systems influenced by disorder or by the proximity to a quantum critical point. © <strong>2010</strong> The American Physical Society.<br />
No.375<br />
Author (s) : Henry, A.; Thongsripong, P.; Fonseca-Gonzalez, I.; Jaramillo-Ocampo, N.; Dujardin, Jean-Pierre<br />
Title : Wing shape <strong>of</strong> dengue vectors from around the world<br />
Source : INFECTION GENETICS AND EVOLUTION, Volume: 10, Issue: 2, Pages: 207-214, DOI: 10.1016/j.meegid.2009.12.001, Published:<br />
MAR <strong>2010</strong><br />
Document Type : Article<br />
Keywords : Aedes aegypti; Ae. albopictus; Colombia; Thailand; Florida; Hawaii; Morphometrics<br />
<strong>Abstract</strong> : Wing shape is increasingly utilized in species identification and characterization. For dengue vectors Aedes aegypti and Aedes<br />
albopictus, it could be used as a complement for ensuring accurate diagnostic <strong>of</strong> damaged specimens. However, the impact<br />
<strong>of</strong> world migration on wing shape is unknown. Has the spread <strong>of</strong> these invasive species increased shape variation to the<br />
extent <strong>of</strong> producing interspecific overlapping? To answer this question, the geometric patterns <strong>of</strong> wing venation in Ae. aegypti<br />
and Ae. albopictus were compared between natural populations from the Pacific Islands, North and South America and South<br />
East Asia. The geometry <strong>of</strong> 178 female and 174 male wings were described at 13 anatomical landmarks, and processed<br />
according to Procrustes superposition, partial warps and subsequent multivariate analyzes. The variation <strong>of</strong> shape did not<br />
produce significant interspecific overlapping. Regardless <strong>of</strong> geographic origin, Ae. aegypti was recognized as Ae. aegypti and Ae.<br />
albopictus as Ae. albopictus. Some significant geographic differentiation was observed in Colombia for Ae. aegypti and in<br />
Thailand for Ae. albopictus. Globally, the morphology <strong>of</strong> these mosquitoes, for both size and shape, appeared well<br />
preserved. Strong canalizing mechanisms could account for the observed patterns <strong>of</strong> relatively uniform morphology, which<br />
could also be attributed to sporadic, recurrent mixing <strong>of</strong> populations, thwarting phenotypic drift. (C) 2009 Elsevier B.V. All<br />
rights reserved.<br />
No.376<br />
Author (s) : Hietala, S.F., Mårtensson, A., Ngasala, B., Dahlström, S., Lindegårdh, N., Annerberg, A., Premji, Z., Färnert, A., Gil, P., Björkman,<br />
A., Ashton, M.<br />
Title : Population pharmacokinetics and pharmacodynamics <strong>of</strong> artemether and lumefantrine during combination treatment in<br />
children with uncomplicated falciparum malaria in Tanzania<br />
Source : Antimicrobial Agents and Chemotherapy, Volume 54, Issue 11, November <strong>2010</strong>, Pages 4780-4788<br />
Document Type : Article<br />
Keywords : amoxicillin, artemether plus benflumetol, cloxacillin, dihydroartemisinin, iron; antimicrobial therapy, article, child, clinical<br />
article, clinical trial, compartment model, controlled study, drug absorption, drug bioavailability, drug clearance, drug<br />
distribution, drug effect, drug elimination, drug fatality, female, host parasite interaction, human, iron therapy, malaria<br />
falciparum, male, nonhuman, preschool child, priority journal, pyrexia idiopathica, school child, skin infection, s<strong>of</strong>t tissue<br />
infection, Tanzania, unspecified side effect; Antimalarials, Artemisinins, Body Temperature, Child, Child,<br />
Preschool, Ethanolamines, Female, Fluorenes, Humans, Infant, Malaria, Falciparum, Male, Tanzania, Treatment Outcome<br />
<strong>Abstract</strong> : The combination <strong>of</strong> artemether (ARM) and lumefantrine is currently the first-line treatment <strong>of</strong> uncomplicated falciparum<br />
malaria in mainland Tanzania. While the exposure to lumefantrine has been associated with the probability <strong>of</strong> adequate<br />
clinical and parasitological cure, increasing exposure to artemether and the active metabolite dihydroartemisinin (DHA) has<br />
been shown to decrease the parasite clearance time. The aim <strong>of</strong> this analysis was to describe the pharmacokinetics and<br />
pharmacodynamics <strong>of</strong> artemether, dihydroartemisinin, and lumefantrine in African children with uncomplicated malaria. In<br />
addition to drug concentrations and parasitemias from 50 Tanzanian children with falciparum malaria, peripheral parasite<br />
densities from 11 asymptomatic children were included in the model <strong>of</strong> the parasite dynamics. The population<br />
pharmacokinetics and pharmacodynamics <strong>of</strong> artemether, dihydroartemisinin, and lumefantrine were modeled in NONMEM.<br />
The distribution <strong>of</strong> artemether was described by a two-compartment model with a rapid absorption and elimination through<br />
metabolism to dihydroartemisinin. Dihydroartemisinin concentrations were adequately illustrated by a one-compartment<br />
model. The pharmacokinetics <strong>of</strong> artemether was time dependent, with typical oral clearance increasing from 2.6 liters/h/kg<br />
on day 1 to 10 liters/h/kg on day 3. The pharmacokinetics <strong>of</strong> lumefantrine was sufficiently described by a one-compartment<br />
138
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
model with an absorption lag time. The typical value <strong>of</strong> oral clearance was estimated to 77 ml/h/kg. The proposed<br />
semimechanistic model <strong>of</strong> parasite dynamics, while a rough approximation <strong>of</strong> the complex interplay between malaria parasite<br />
and the human host, adequately described the early effect <strong>of</strong> ARM and DHA concentrations on the parasite density in malaria<br />
patients. However, the poor precision in some parameters illustrates the need for further data to support and refine this<br />
model. Copyright © <strong>2010</strong>, American Society for Microbiology. All Rights Reserved.<br />
No.377<br />
Author (s) : Hodak, S.K., Supasai, T., Wisitsoraat, A., Hodak, J.H.<br />
Title : Design <strong>of</strong> low cost gas sensor based on SrTiO3 and BaTiO 3 films<br />
Source : Journal <strong>of</strong> Nanoscience and Nanotechnology, Volume 10, Issue 11, November <strong>2010</strong>, Pages 7236-7238<br />
Document Type : Conference Paper<br />
Keywords : Gas sensor; Sol-gel method; SrTiO3/BaTiO3<br />
<strong>Abstract</strong> : We have prepared SrTiO3/BaTiO3 multilayer film on alumina substrates by a sol-gel technique and investigated their response<br />
for sensing ethanol vapor. The surface morphology <strong>of</strong> the films were characterized by atomic force microscope (AFM) showing<br />
that the grain size <strong>of</strong> the films increase up to 40 nm as the annealing temperature increased to 1000 ̈C. The ethanol sensors<br />
based on SrTiO3/BaTiO3 thin films were fabricated by applying interdigitated gold electrodes by sputtering technique. The<br />
ethanol sensing characteristics <strong>of</strong> SrTiO3/BaTiO3 thin films were quantified by the change in resistance <strong>of</strong> the sensors when<br />
they were exposed to ethanol. The optimum operating tempearature <strong>of</strong> these sensors was found to be 350 °C. In addition,<br />
the film annealed at 1000 ̈C exhibited p-type gas sensing behavior with the best sensitivity <strong>of</strong> 30-100 for low ethanol<br />
concentration in the range <strong>of</strong> 100-1000 ppm . Copyright © <strong>2010</strong> American Scientific Publishers.<br />
No.378<br />
Author (s) : Hollenbach, J.A., Meenagh, A., Sleator, C., Alaez, C., Bengoche, M., Canossi, A., Contreras, G., Creary, L., Evseeva, I., Gorodezky,<br />
C., Hardie, R.-A., Hemming Karlsen, T., Lie, B., Luo, M., Martinetti, M., Navarette, C., De Oliveira, D.C.M., Ozzella, G., Pasi, A.,<br />
Pavlova, E., Pinto, S., Porto, L.C., Santos, P., Slavcev, A., Srinak, D., Tavoularis, S., Tonks, S., Trachtenberg, E., Vejbaesya, S.,<br />
Middleton, D.<br />
Title : Report from the killer immunoglobulin-like receptor (KIR) anthropology component <strong>of</strong> the 15th international<br />
histocompatibility workshop: Worldwide variation in the KIR loci and further evidence for the co-evolution <strong>of</strong> KIR and HLA<br />
Source : Tissue Antigens, Volume 76, Issue 1, July <strong>2010</strong>, Pages 9-17<br />
Document Type : Article<br />
Keywords : 15th <strong>International</strong> Histocompatibility Workshop; Anthropology; Killer immunoglobulin-like receptor; Ligand<br />
<strong>Abstract</strong> : The killer immunoglobulin-like receptor (KIR) anthropology component <strong>of</strong> the 15th <strong>International</strong> Histocompatibility Workshop<br />
(IHIWS) sought to explore worldwide population variation in the KIR loci, and to examine the relationship between KIR genes<br />
and their human leukocyte antigen (HLA) ligands. Fifteen laboratories submitted KIR genotype and HLA ligand data in 27<br />
populations from six broad ethnic groups. Data were analyzed for correlations between the frequencies <strong>of</strong> KIR and their<br />
known HLA ligands. In addition, allelic typing was performed for KIR2DL2 and 3DL1 in a subset <strong>of</strong> populations. Strong and<br />
significant correlations were observed between KIR2DL2, 2DL3 genotype frequencies and the frequency <strong>of</strong> their ligand, HLA-<br />
C1. In contrast, only weak associations were seen for 3DL1, 3DS1 and the HLA-Bw4 ligand. Although some aspects <strong>of</strong> the<br />
correlations observed here differ from those reported in other populations, these data provide additional evidence <strong>of</strong> linked<br />
evolutionary histories for some KIR and HLA loci. Investigation <strong>of</strong> allele-level variation for the B haplotype locus KIR 2DL2<br />
showed that two alleles, *001 and *003, predominate in all populations in this study. Much more allelic variation was<br />
observed for the A haplotype locus 3DL1, with several alleles observed at moderate frequencies and extensive variation<br />
observed between populations. © <strong>2010</strong> John Wiley & Sons A/S.<br />
No.379<br />
Author (s) : Homhuan, P., Chaiyakun, S., Thonggoom, R., Panich, N., Tungasmita, S.<br />
Title : Growth and structural characterizations <strong>of</strong> nanostructured chromium-zirconium-nitride thin films for tribological applications<br />
Source : Materials Transactions, Volume 51, Issue 9, September <strong>2010</strong>, Pages 1651-1655<br />
Document Type : Article<br />
Keywords : Hard coating; Magnetron sputtering; Nanostructure; Protective thin films<br />
<strong>Abstract</strong> : Nanostructured Cr-Zr-N thin films were grown on Si(100) substrates in a mixture <strong>of</strong> Ar and N2 plasma. The nitrogen partial<br />
pressure was varied to produce and control the stoichiometric forms obtained. All the Cr-Zr-N films exhibited a nanostructure<br />
139
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
with an average grain size <strong>of</strong> less than 10 nm, as determined by X-ray diffractogram analysis, and were formed in the solidsolution.<br />
As the contents <strong>of</strong> nitrogen in the film increased, it lead to changes in the crystal texture and competitive growth.<br />
The maximum root mean square roughness was 7.87 nm at a 20% nitrogen partial pressure and the roughness tended to<br />
decrease as the grain size decreased. The nano-indentation showed that the films grown at a 20% nitrogen partial pressure<br />
and annealed at 700°C had the highest reduced modulus and hardness at 349.2 and 35.1 GPa, respectively. The mechanical<br />
properties <strong>of</strong> films can be improved by a post-annealing heat treatment. With respect to the electrical properties <strong>of</strong> these<br />
films, the sheet resistance, which is related to the defect level, tended to increase as the nitrogen partial pressure increased.<br />
© <strong>2010</strong> The Japan Institute <strong>of</strong> Metals.<br />
No.380<br />
Author (s) : Hongeng, S.; Nuchprayoon, I.; Seksarn, P.; Wiangnon, S.; Veerakul, G.; Krutvechos, T.<br />
Title : NATIONAL PEDIATRIC BRAIN TUMOR REGISTRY IN THAILAND: REPORT FROM THAI PEDIATRIC ONCOLOGY GROUP (2003-2005)<br />
Source : NEURO-ONCOLOGY, Volume: 12, Issue: 6, Pages: II45-II46, Published: JUN <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.381<br />
Author (s) : Hongkacharn, T., Champreda, V., Srikhirin, T., Wangkam, T., Osotchan, T.<br />
Title : Kinetic study <strong>of</strong> protein formation and digestion by quartz crystal microbalance<br />
Source : INEC <strong>2010</strong> - <strong>2010</strong> 3rd <strong>International</strong> Nanoelectronics Conference, Proceedings, <strong>2010</strong>, Article number 5424730, Pages 338-339<br />
Document Type : Conference Paper<br />
Keywords : Bovine serum albumins, Concentration effects, Concentration <strong>of</strong>, Frequency shift, Function <strong>of</strong> time, Kinetic study, Layer<br />
formation, Maleic acids, Protein formation, Quartz crystal microbalance techniques, Resistance shifts; Body<br />
fluids, Concentration (process), Nanoelectronics, Oxide minerals, Piezoelectric devices, Polystyrenes, Proteins, Quartz, Quartz<br />
crystal microbalances; pH effects<br />
<strong>Abstract</strong> : Quartz crystal microbalance technique was used to study kinetic <strong>of</strong> formation and digestion <strong>of</strong> bovine serum albumin (BSA)<br />
protein on polystyrene-co-maleic acids (PSMA) surface. The kinetic <strong>of</strong> formation and digestion <strong>of</strong> BSA was investigated by<br />
measuring the frequency shift and resistance shift as a function <strong>of</strong> time. In order to study pH and concentration effect to layer<br />
formation kinetic, the pH <strong>of</strong> solution was varied from 2.0 to 7.4 while the concentration <strong>of</strong> BSA was varied in the range <strong>of</strong><br />
0.001 to 10 mg/ml. The kinetic <strong>of</strong> formation appears to be sensitive to the pH <strong>of</strong> solution and concentration. The formation<br />
layer at about pH 3.0 to 3.5 gives the different characteristic from the others. The layer formation increases as the<br />
concentration increase then reached the saturate value at the concentration <strong>of</strong> over 3 mg/ml. The kinetic <strong>of</strong> digestion was<br />
evaluate by applying proteinase enzyme on varies densities <strong>of</strong> BSA layer. It found that the rate <strong>of</strong> digestion depends on the<br />
density <strong>of</strong> the molecular BSA packing, modified by varying pH and concentration <strong>of</strong> BSA. ©<strong>2010</strong> IEEE.<br />
No.382<br />
Author (s) : Hongkachern, T., Champreda, V., Srikhirin, T., Wangkam, T., Osotchan, T.<br />
Title : Effect <strong>of</strong> pH on the formation <strong>of</strong> a bovine serum albumin layer on a poly(stryren-co-maleic acid) surface<br />
Source : Advanced Materials Research, Volume 93-94, <strong>2010</strong>, Pages 583-586<br />
Document Type : Conference Paper<br />
Keywords : Bovine serum albumin; Protein layer formation; Quartz crystal microbalance<br />
<strong>Abstract</strong> : The layer formation <strong>of</strong> bovine serum albumin (BSA) on a poly(styrene-co- maleic acid) (PSMA) surface was investigated by<br />
using quartz crystal microbalance (QCM) technique at various pH values. The formation <strong>of</strong> a BSA surface was examined by<br />
atomic force microscopy (AFM). To study the effect on the layer formation, the pH <strong>of</strong> solution was varied from 2 to 7.4 while<br />
the concentration <strong>of</strong> BSA was in the range <strong>of</strong> 0.01 to 5 mg/ml during the layer absorption. It was found that the BSA<br />
adsorption strongly depends on the pH <strong>of</strong> solution, and the concentration <strong>of</strong> BSA. The absorption layer occurred maximum at<br />
the pH value <strong>of</strong> 3.5 which resulted from the charge <strong>of</strong> PSMA and BSA molecules. The layer formation reached the saturate<br />
value at the concentration higher than 3 mg/ml. The molecular packing <strong>of</strong> the BSA layer at different pH values was<br />
determined by AFM and total mass change <strong>of</strong> QCM. © (<strong>2010</strong>) Trans Tech <strong>Publications</strong>.<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.383<br />
Author (s) : Hongsith, N., Wongrat, E., Kerdcharoen, T., Choopun, S.<br />
Title : Sensor response formula for sensor based on ZnO nanostructures<br />
Source : Sensors and Actuators, B: Chemical, Volume 144, Issue 1, 29 January <strong>2010</strong>, Pages 67-72<br />
Document Type : Article<br />
Keywords : Gas sensor; Nanostructure; Sensor response formula; ZnO<br />
<strong>Abstract</strong> : In this paper, we propose a new and general formula to describe ethanol adsorption mechanism underlying the response<br />
enhancement <strong>of</strong> ZnO nanostructure sensors. The derivation <strong>of</strong> sensor response formula based on basic chemical reaction at<br />
the sensor surface is presented. The formula can be used to explain response enhancement due to effect <strong>of</strong> metal doping,<br />
surface-to-volume ratio, and surface depletion layer. Thus, it can be regarded as a general formula to describe the sensor<br />
response characteristics <strong>of</strong> ZnO sensors. This general formula is a powerful tool for designing ZnO sensor at any desired<br />
sensor response. Furthermore, it is reasonable to expand this formula to explain other sensing materials and also to explain<br />
for different active gases. © 2009 Elsevier B.V. All rights reserved.<br />
No.384<br />
Author (s) : Horby, P., Sudoyo, H., Viprakasit, V., Fox, A., Thai, P.Q., Yu, H., Davila, S., Hibberd, M., Dunstan, S.J., Monteerarat, Y., Farrar, J.J.,<br />
Marzuki, S., Hien, N.T.<br />
Title : What is the evidence <strong>of</strong> a role for host genetics in susceptibility to influenza A/H5N1?<br />
Source : Epidemiology and Infection, Volume 138, Issue 11, November <strong>2010</strong>, Pages 1550-1558<br />
Document Type : Review<br />
Keywords : genetic susceptibility; H5N1 subtype; Influenza A virus<br />
<strong>Abstract</strong> : The apparent family clustering <strong>of</strong> avian influenza A/H5N1 has led several groups to postulate the existence <strong>of</strong> a host genetic<br />
influence on susceptibility to A/H5N1, yet the role <strong>of</strong> host factors on the risk <strong>of</strong> A/H5N1 disease has received remarkably little<br />
attention compared to the efforts focused on viral factors. We examined the epidemiological patterns <strong>of</strong> human A/H5N1<br />
cases, their possible explanations, and the plausibility <strong>of</strong> a host genetic effect on susceptibility to A/H5N1 infection. The<br />
preponderance <strong>of</strong> familial clustering <strong>of</strong> cases and the relative lack <strong>of</strong> non-familial clusters, the occurrence <strong>of</strong> related cases<br />
separated by time and place, and the paucity <strong>of</strong> cases in some highly exposed groups such as poultry cullers, are consistent<br />
with a host genetic effect. Animal models support the biological plausibility <strong>of</strong> genetic susceptibility to A/H5N1. Although the<br />
evidence is circumstantial, host genetic factors are a parsimonious explanation for the unusual epidemiology <strong>of</strong> human<br />
A/H5N1 cases and warrant further investigation. Copyright © <strong>2010</strong> Cambridge <strong>University</strong> Press.<br />
No.385<br />
Author (s) : Hoshino, J., Limpanart, S., Khunthon, S., Osotchan, T., Traiphol, R., Srikhirin, T.<br />
Title : Adsorption <strong>of</strong> single-strand alkylammonium salts on bentonite, surface properties <strong>of</strong> the modified clay and polymer<br />
nanocomposites formation by a two-roll mill<br />
Source : Materials Chemistry and Physics, Volume 123, Issue 2-3, 1 October <strong>2010</strong>, Pages 706-713<br />
Document Type : Article<br />
Keywords : Bentonite; Montmorillonite; Nanocomposites; Organoclay; Polyethylene<br />
<strong>Abstract</strong> : The adsorption <strong>of</strong> tallow alkylammonium salts onto bentonite from the Lopburi province in Thailand, and the effect <strong>of</strong><br />
surface wettability on the formation <strong>of</strong> polymer-clay nanocomposites are reported. We looked specifically at<br />
octadecyltrimethyl ammonium chloride (S18), a popular member <strong>of</strong> the tallow alkylammonium salt family. The adsorption <strong>of</strong><br />
S18 onto the bentonite interlayer can be divided into three distinct stages depending upon the initial concentration <strong>of</strong> S18.<br />
These stages are (a) monolayer formation, (b) intermediate state <strong>of</strong> double layer formation and (c) double layer formation. A<br />
decrease in surface energy driven by drops in the values <strong>of</strong> the polar and dispersive components was observed as the<br />
amount <strong>of</strong> surfactant surface coverage increased. Using thermal gravimetric analysis (TGA), the critical surface energy (CSE) <strong>of</strong><br />
organoclay was found to decrease as the amount <strong>of</strong> absorbed S18 increased. S18 organoclay with different degrees <strong>of</strong> surface<br />
coverage was used in a two-roll mill to prepare high-density polyetheylene (HDPE)-clay nanocomposites. The oxidized<br />
polyethylene wax (OWax) was used as a dispersing agent to promote the delamination <strong>of</strong> the organoclay platelets. The<br />
results from X-ray diffraction (XRD) and transmission electron microscope (TEM) indicated a difference in the dispersing<br />
capability <strong>of</strong> the organoclay. © <strong>2010</strong> Elsevier B.V. All rights reserved.<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.386<br />
Author (s) : Hosono, N., Chantarangsu, S., Kiyotani, K., Takata, S., Tsuchiya, Y., Mahasirimongkol, S., Chantratita, W., Mushiroda, T.,<br />
Nakamura, Y., Kubo, M.<br />
Title : Development <strong>of</strong> new HLA-B*3505 genotyping method using invader assay<br />
Source : Pharmacogenetics and Genomics, Volume 20, Issue 10, October <strong>2010</strong>, Pages 630-633<br />
Document Type : Article<br />
Keywords : drug hypersensitivity; HIV/AIDS; HLA-B*3505; SSP-PCR; universal Invader assay<br />
<strong>Abstract</strong> : Several pharmacogenetic studies have revealed strong associations between specific human leukocyte antigen (HLA) alleles<br />
and the susceptibility to drug hypersensitivity. Recently, we reported HLA-B*3505 as a strong genetic biomarker for the<br />
nevirapine-induced skin rash in Thai population. Here, we developed a new HLA-B*3505 genotyping method by a combination<br />
<strong>of</strong> the Universal Invader assay and sequence-specific primer PCR. From the sequence alignment <strong>of</strong> 68 HLA-B alleles in the<br />
Thai population, we selected the two most discriminative SNPs (rs1140412 and rs4997052) as target SNP sites. When we<br />
carried out the assay using 324 Thai individuals, fluorescence intensities <strong>of</strong> HLA-B*3505-positive and HLA-B*3505-negative<br />
samples were apparently discriminated at the endpoint <strong>of</strong> the reaction. Our results were 100% concordant with those<br />
obtained by a sequence-based typing method. As our assay is simple and rapid, we believe our method will be a useful tool<br />
for pharmacogenetic testing <strong>of</strong> the nevirapine-induced skin rash. © <strong>2010</strong> Wolters Kluwer Health | Lippincott Williams & Wilkins.<br />
No.387<br />
Author (s) : Huabsomboon, P., Novaprateep, B., Kaneko, H.<br />
Title : On Taylor-series expansion methods for the second kind integral equations<br />
Source : Journal <strong>of</strong> Computational and Applied Mathematics, Volume 234, Issue 5, 1 July <strong>2010</strong>, Pages 1466-1472<br />
Document Type : Article<br />
Keywords : Fredholm integral equation; System <strong>of</strong> integral equations; Taylor method<br />
<strong>Abstract</strong> : In this paper, we comment on the recent papers by Yuhe Ren et al. (1999) [1] and Maleknejad et al. (2006) [7] concerning the<br />
use <strong>of</strong> the Taylor series to approximate a solution <strong>of</strong> the Fredholm integral equation <strong>of</strong> the second kind as well as a solution<br />
<strong>of</strong> a system <strong>of</strong> Fredholm equations. The technique presented in Yuhe Ren et al. (1999) [1] takes advantage <strong>of</strong> a rapidly<br />
decaying convolution kernel k (| s - t |) as | s - t | increases. However, it does not apply to equations having other types <strong>of</strong><br />
kernels. We present in this paper a more general Taylor expansion method which can be applied to approximate a solution<br />
<strong>of</strong> the Fredholm equation having a smooth kernel. Also, it is shown that when the new method is applied to the Fredholm<br />
equation with a rapidly decaying kernel, it provides more accurate results than the method in Yuhe Ren et al. (1999) [1]. We<br />
also discuss an application <strong>of</strong> the new Taylor-series method to a system <strong>of</strong> Fredholm integral equations <strong>of</strong> the second kind.<br />
© <strong>2010</strong> Elsevier B.V. All rights reserved.<br />
No.388<br />
Author (s) : Huguenin, M., Bracha, R., Chookajorn, T., Mirelman, D.<br />
Title : Epigenetic transcriptional gene silencing in Entamoeba histolytica: Insight into histone and chromatin modifications<br />
Source : Parasitology, Volume 137, Issue 4, April <strong>2010</strong>, Pages 619-627<br />
Document Type : Article<br />
Keywords : Chromatin modifications; Entamoeba histolytica; Epigenetic silencing; Heterochromatin; Histone methylation<br />
<strong>Abstract</strong> : We have previously discovered a unique mechanism <strong>of</strong> epigenetic transcriptional gene silencing in the Entamoeba histolytica<br />
trophozoites <strong>of</strong> strain HM-1:IMSS that resulted in the persistent downregulation <strong>of</strong> the amoebapore A (ap-a) gene, and that<br />
could be successfully applied to silence other virulence genes (cpA5, lgl1). In order to understand how the silencing is<br />
maintained throughout generations, we analysed whether modifications occurred at the chromatin level. Chromatin<br />
immunoprecipitation assays were done with antibodies specific to the methylated lysine 4 <strong>of</strong> E. histolytica histone H3. When<br />
the genes were in a transcriptionally silent state, the methylation levels <strong>of</strong> H3K4 in their coding region were significantly<br />
reduced. In contrast, the levels <strong>of</strong> core histone H3 were consistently higher in the silenced genes. Controlled chromatin<br />
digestion with micrococcal nuclease was used to assess changes in nucleosome compaction. We found a significant resistance<br />
to digestion in the promoter region <strong>of</strong> the silenced ap-a and cpA5 genes as compared to the parental strain that expresses<br />
those genes. Our data lend further support to the idea that histone modifications and heterochromatin formations are at the<br />
basis <strong>of</strong> the transcriptional silencing <strong>of</strong> genes in E. histolytica. © 2009 Cambridge <strong>University</strong> Press.<br />
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No.389<br />
Author (s) : Hunt, B.<br />
Title : Risk and Crisis Management in the Public Sector<br />
Source : PUBLIC MANAGEMENT REVIEW, Volume: 12, Issue: 5, Pages: 747-751, Article Number: PII 926994322, Published: 201<br />
Document Type : Book Review<br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.390<br />
Author (s) : Huntula, J., Chitaree, R.<br />
Title : Improve students' awareness <strong>of</strong> linking the experiment with their real lives<br />
Source : AIP Conference Proceedings, Volume 1263, <strong>2010</strong>, Pages 55-57<br />
Document Type : Conference Paper<br />
Keywords : physics laboratory; physics laboratory direction<br />
<strong>Abstract</strong> : We surveyed 218 science students' from a Thai <strong>University</strong> for their views about what should be done to improve the<br />
introductory physics laboratory course. One <strong>of</strong> their responses strongly recommended that the real life application contents<br />
to the experiment should be indicated in the physics laboratory direction. The inclusions should give them a clear reason<br />
how the thing they learn from the experiment can probably be used in their lives. From our survey, about 83% <strong>of</strong> students<br />
agreed that the laboratory instruction should include an example <strong>of</strong> real life situation. Therefore, our initial goal was to find<br />
an appropriate way to improve students' awareness <strong>of</strong> linking what they learn from the experiment with their real life<br />
experiences. In the first semester <strong>of</strong> 2008, the first trial <strong>of</strong> modified physics laboratory direction was carried out with 18<br />
second year physics students. The additional contents <strong>of</strong> physics applications were introduced as the prolog <strong>of</strong> the physics<br />
laboratory direction. Four out <strong>of</strong> twelve experiment directions were prepared to include this additional introduction. From our<br />
interview as a mean to evaluate the proposal, only 11% <strong>of</strong> students could explain but their answers disagreed with the<br />
examples <strong>of</strong> real life situations given within the experiment direction. This result made us realized that this was not only the<br />
matter <strong>of</strong> having or not having the application messages but also the matter <strong>of</strong> putting the massages in the right place. In the<br />
second semester <strong>of</strong> 2008, the second trial was carried out. This time, the application contents were blended into the<br />
theoretical part which was found from our separate survey to be one <strong>of</strong> the most interesting parts for students. Again, four<br />
out <strong>of</strong> twelve experiment directions were prepared in this proposed style. The students' responses showed that about 40% <strong>of</strong><br />
students could clearly describe the application message relevant to experiments given in the direction. © <strong>2010</strong> American<br />
Institute <strong>of</strong> Physics.<br />
No.391<br />
Author (s) : Hutspardol, S., Chuansamrit, A., Soisamrong, A.<br />
Title : Spontaneous subgaleal hemorrhage in a girl with impaired adrenaline-induced platelet aggregation<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 5, May <strong>2010</strong>, Pages 625-628<br />
Document Type : Article<br />
Keywords : Impaired adrenaline-induced platelet aggregation; Spontaneous subgaleal hematoma<br />
<strong>Abstract</strong> : Introduction: Subgaleal hematomas usually develop followed a birth trauma in neonates. This entity is extremely rare in<br />
older children and may be associated with coagulation disorders or subaponeurotic vascular malformations. Case Report: The<br />
authors report a spontaneous subgaleal hematoma in a 9-year-old girl without scalp injury. Only platelet aggregation test was<br />
identified as an impaired responsiveness <strong>of</strong> platelets to adrenaline with normal coagulogram. This patient underwent both<br />
computerized tomography and complete magnetic resonance imaging including angiography and venography <strong>of</strong> the head. Of<br />
these, extensive bilateral frontotemperoparietal subgaleal hematoma was observed without vascular malformation.<br />
Conclusion: Spontaneous subgaleal hematoma can be associated with platelet function defect.<br />
No.392<br />
Author (s) : Hutspardol, S., Sirachainan, N., Soisamrong, A., Atchararit, N., O-Prasertsawat, P., Chuansumrit, A.<br />
Title : Hemostatic defects in thai adolescents with menorrhagia<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 4, April <strong>2010</strong>, Pages 436-442<br />
Document Type : Article<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : Adrenaline receptor defect; Hemostatic disorders; Menorrhagia; Pictorial blood loss assessment chart (PBAC); Thai<br />
adolescents<br />
<strong>Abstract</strong> : Twenty-eight adolescents with menorrhagia by pictorial blood loss assessment chart (PBAC) criteria were investigated for<br />
underlying hemostatic defect. CBC, ABO blood group, bleeding time, APTT, PT, TT, FVIII:C, VWF:Ag, RiCoF and platelet<br />
aggregation study were evaluated. Six patients (21.4%) were addressed with underlying hemostatic defect. Of these, severe<br />
aplastic anemia (n = 1) and thrombotic thrombocytopenic purpura (n = 1) were identified in 2 patients with low platelets<br />
after an initial CBC. Four patients with prolonged bleeding time demonstrated inherited hemostatic defect: von Willebrand<br />
disease (VWD) type 3 (n = 1), Glanzmann thrombasthenia (n = 1) and Bernard-Soulier syndrome (n = 2). Median PBAC score <strong>of</strong><br />
patients with hemostatic defect was significantly higher than that <strong>of</strong> patients with unknown cause <strong>of</strong> menorrhagia (436.5 vs.<br />
251.3, p = 0.01). After the exclusion <strong>of</strong> six patients with well-identified bleeding risks, isolated abnormal platelet aggregation<br />
response to adrenaline was detected in 11 (50%) adolescents using platelet aggregation study. No significant difference <strong>of</strong><br />
median PBAC score was noted among patients with and without evidence <strong>of</strong> this impaired responsiveness to adrenaline. In<br />
addition, the authors also found an abnormal platelet aggregation with adrenaline stimulant in 15 (75%) among 20 healthy<br />
female controls who had no history <strong>of</strong> bleeding diathesis. No significant difference in frequency <strong>of</strong> abnormal platelet<br />
aggregation to adrenaline was observed between affected cases and controls. In summary, an impaired responsiveness <strong>of</strong><br />
platelets to adrenaline in the present study is insufficient to support its risk <strong>of</strong> bleeding. On the contrary, the simple test such<br />
as CBC and bleeding time revealed a worthy contribution to investigate coexisting coagulopathy in adolescents with<br />
menorrhagia.<br />
No.393<br />
Author (s) : Hutspardol, S., Pakakasama, S., Hongeng, S., Subhadrabandhu, T., Samankatiwat, P.<br />
Title : INITIAL BILATERAL LUNG METASTASES INFLUENCED LOCAL RECURRENCE, PROGRESSION FREE SURVIVAL AND OVERALL<br />
SURVIVAL IN PATIENTS WITH OSTEOSARCOMA<br />
Source : PEDIATRIC BLOOD & CANCER, Volume: 55, Issue: 5, Pages: 876-876, Published: NOV <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.394<br />
Author (s) : Hüttinger, F., Satimai, W., Wernsdorfer, G., Wiedermann, U., Congpuong, K., Wernsdorfer, W.H.<br />
Title : Sensitivity to artemisinin, mefloquine and quinine <strong>of</strong> Plasmodium falciparum in northwestern Thailand<br />
Source : Wiener Klinische Wochenschrift, Volume 122, Issue SUPPL. 3, October <strong>2010</strong>, Pages 52-56<br />
Document Type : Article<br />
Keywords : Artemisinin; Drug sensitivity; Mefloquine; Plasmodium falciparum; Quinine; Thailand<br />
<strong>Abstract</strong> : The increasing drug resistance <strong>of</strong> Plasmodium falciparum is a worldwide problem. The objective <strong>of</strong> the study was the<br />
assessment <strong>of</strong> the in vitro activity <strong>of</strong> artemisinin, mefloquine and quinine, in an area where P. falciparum is multi-drug<br />
resistant. The sensitivity tests were based on measuring the drug-dependent inhibition <strong>of</strong> schizont maturation. For the 43<br />
successfully tested isolates the mean effective concentrations (IC50 and IC 90) for artemisinin were 0.0081 and 0.1372 μM,<br />
respectively. For mefloquine the IC50 was 0.1260 μM and the IC90 was 3.7345 μM. Quinine showed an IC50 <strong>of</strong> 0.2155 μM<br />
and an IC 90 <strong>of</strong> 2.5040 μM. All tested drugs showed a significant reduction in the effectiveness, compared with the results <strong>of</strong><br />
former years. This suggests a further rise <strong>of</strong> resistance <strong>of</strong> local P. falciparum, which is alarming especially for artemisinin and<br />
quinine. © <strong>2010</strong> Springer-Verlag.<br />
No.395<br />
Author (s) : Iamaroon, A., Raksakietisak, M., Halilamien, P., Hongsawad, J., Kwankamol, Boonsararuxsapong<br />
Title : Femoral nerve block versus fentanyl: Analgesia for positioning patients with fractured femur<br />
Source : Local and Regional Anesthesia, Volume 3, Issue 1, <strong>2010</strong>, Pages 21-26<br />
Document Type : Article<br />
Keywords : Bupivacaine; Femoral nerve block; Fentanyl; Pain on positioning<br />
<strong>Abstract</strong> : Purpose: Fracture <strong>of</strong> femur is a painful bone injury, worsened by any movement. This prospective study was performed to<br />
compare the analgesic effects <strong>of</strong> femoral nerve block (FNB) with intravenous (IV) fentanyl prior to positioning patients with<br />
fractured femur for spinal block. Patients and methods: Sixty-four ASA I-III patients aged 18-80 years undergoing surgery for<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
femur fracture were randomized into two groups. Fifteen minutes before spinal block, the FNB group received nerve<br />
stimulator-assisted FNB with a mixture <strong>of</strong> 20 mL bupivacaine 0.5% and 10 mL normal saline 0.9%, and the fentanyl group<br />
received two doses <strong>of</strong> IV fentanyl 0.5 μg/kg with a five-minute interval between doses. Numeric rating pain scores were<br />
compared. During positioning, fentanyl in 0.5 μg/kg increments was given every five minutes until pain scores were ≤4.<br />
Results: There were no statistically significant differences between the groups according to pain scores, need for additional<br />
fentanyl, and satisfaction with positioning before spinal block. Conclusion: We were unable to demonstrate a benefit <strong>of</strong> FNB<br />
over IV fentanyl for patient positioning before spinal block. However, FNB can provide postoperative pain relief, whereas side<br />
effects <strong>of</strong> fentanyl must be considered, and analgesic dosing should be titrated based on pain scores. A multimodal approach<br />
(FNB + IV fentanyl) may be a possible option. © <strong>2010</strong> Iamaroon et al, publisher and licensee Dove Medical Press Ltd.<br />
No.396<br />
Author (s) : Iamsawat, S., Surawut, S., Prammananan, T., Leelaporn, A., Jearanaisilavong, J.<br />
Title : Multiplex PCR for detection <strong>of</strong> clarithromycin resistance and simultaneous species identification <strong>of</strong> Mycobacterium avium<br />
complex<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 3, May <strong>2010</strong>, Pages 590-601<br />
Document Type : Article<br />
Keywords : Clarithromycin resistance; M. avium complex; Mycobacterium; PCR<br />
<strong>Abstract</strong> : Multiplex PCR (mPCR) was established for the simultaneous detection <strong>of</strong> clarithromycin (CLR) resistance and species<br />
identification <strong>of</strong> Mycobacterium avium complex (MAC). mPCR was tested on 218 MAC clinical isolates. CLR-resistance was<br />
detected by mPCR in 31 <strong>of</strong> 35 isolates identified by a microdilution method. Of the remaining 187 susceptible isolates<br />
identified by mPCR, 183 isolates had MIC ≤8 μg/ml (susceptible), 3 with MIC <strong>of</strong> 16 (intermediate resistant) and 1 with MIC <strong>of</strong> ≥<br />
32 μg/ml (resistant). Comparing with the PCR-restriction enzyme analysis, mPCR concordantly identified 185 isolates either as<br />
being M. avium or M. intracellulare, whereas one isolate was misidentified and 32 isolates could not be identified. Comparing<br />
with reference methods, the mPCR showed the sensitivity, specificity, positive predictive and negative predictive value <strong>of</strong> 89,<br />
100, 100, and 98% for detection <strong>of</strong> CLR resistance; 92, 98, 99, and 78% for identification <strong>of</strong> M. avium; and 57, 100, 100, and<br />
89% for identification <strong>of</strong> M. intracellulare, respectively.<br />
No.397<br />
Author (s) : Igarashi, Y., Shimasaki, R., Miyanaga, S., Oku, N., Onaka, H., Sakurai, H., Saiki, I., Kitani, S., Nihira, T., Wimonsiravude, W.,<br />
Panbangred, W.<br />
Title : Rakicidin D, an inhibitor <strong>of</strong> tumor cell invasion from marine-derived Streptomyces sp.<br />
Source : Journal <strong>of</strong> Antibiotics, Volume 63, Issue 9, September <strong>2010</strong>, Pages 563-565<br />
Document Type : Article<br />
Keywords : invasion inhibitor; rakicidin; Streptomyces<br />
<strong>Abstract</strong> : –<br />
No.398<br />
Author (s) : Imwong, M., Dondorp, A.M., Nosten, F., Yi, P., Mungthin, M., Hanchana, S., Das, D., Phyo, A.P., Lwin, K.M., Pukrittayakamee, S.,<br />
Lee, S.J., Saisung, S., Koecharoen, K., Nguon, C., Day, N.P.J., Socheat, D., White, N.J.<br />
Title : Exploring the contribution <strong>of</strong> candidate genes to artemisinin resistance in plasmodium falciparum<br />
Source : Antimicrobial Agents and Chemotherapy, Volume 54, Issue 7, July <strong>2010</strong>, Pages 2886-2892<br />
Document Type : Article<br />
Keywords : adenosine triphosphatase, artemisinin, artesunate plus mefloquine, atpase 6, dihydroartemisinin plus piperaquine, unclassified<br />
drug; antimalarial drug resistance, article, Cambodia, gene, gene amplification, gene dosage, gene mutation, MDR1<br />
gene, mitochondrial genome, nonhuman, nucleotide sequence, Plasmodium falciparum, priority journal, Thailand, ubp 1<br />
gene; Animals, Anti-Infective Agents, Artemisinins, Calcium-Transporting ATPases, Drug Resistance, Models, Biological, Multidrug<br />
Resistance-Associated Proteins, Plasmodium falciparum, Polymerase Chain Reaction, Protozoan Proteins<br />
<strong>Abstract</strong> : The reduced in vivo sensitivity <strong>of</strong> Plasmodium falciparum has recently been confirmed in western Cambodia. Identifying<br />
molecular markers for artemisinin resistance is essential for monitoring the spread <strong>of</strong> the resistant phenotype and identifying<br />
the mechanisms <strong>of</strong> resistance. Four candidate genes, including the P. falciparum mdr1 (pfmdr1) gene, the P. falciparum<br />
ATPase6 (pfATPase6) gene, the 6-kb mitochondrial genome, and ubp-1, encoding a deubiquitinating enzyme, <strong>of</strong> artemisininresistant<br />
P. falciparum strains from western Cambodia were examined and compared to those <strong>of</strong> sensitive strains from<br />
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northwestern Thailand, where the artemisinins are still very effective. The artemisinin-resistant phenotype did not correlate<br />
with pfmdr1 amplification or mutations (full-length sequencing), mutations in pfATPase6 (full-length sequencing) or the 6-kb<br />
mitochondrial genome (full-length sequencing), or ubp-1 mutations at positions 739 and 770. The P. falciparum CRT K76T<br />
mutation was present in all isolates from both study sites. The pfmdr1 copy numbers in western Cambodia were significantly<br />
lower in parasite samples obtained in 2007 than in those obtained in 2005, coinciding with a local change in drug policy<br />
replacing artesunate-mefloquine with dihydroartemisinin-piperaquine. Artemisinin resistance in western Cambodia is not<br />
linked to candidate genes, as was suggested by earlier studies. Copyright © <strong>2010</strong>, American Society for Microbiology. All Rights<br />
Reserved.<br />
No.399<br />
Author (s) : Inderbitzin, P., Asvarak, T., Turgeon, B.G.<br />
Title : Six new genes required for production <strong>of</strong> T-toxin, a polyketide determinant <strong>of</strong> high virulence <strong>of</strong> Cochliobolus heterostrophus<br />
to maize<br />
Source : Molecular Plant-Microbe Interactions, Volume 23, Issue 4, April <strong>2010</strong>, Pages 458-472<br />
Document Type : Article<br />
Keywords : fungal protein, mycotoxin, Tox1 protein, Cochliobolus heterostrophus; article, Ascomycetes, chromosome, chromosome<br />
map, gene deletion, gene expression<br />
regulation, genetics, maize, metabolism, microbiology, pathogenicity, phylogeny, physiology, plant disease, virulence;<br />
Ascomycota, Chromosome Mapping, Chromosomes, Fungal, Fungal Proteins, Gene Deletion, Gene Expression Regulation,<br />
Fungal, Mycotoxins, Phylogeny, Plant Diseases, Virulence, Zea mays; Ascomycota, Cochliobolus, Cochliobolus<br />
heterostrophus, Delitschia, Mycosphaerella zeae-maydis, Zea mays<br />
<strong>Abstract</strong> : Southern Corn Leaf Blight, one <strong>of</strong> the worst plant disease epidemics in modern history, was caused by Cochliobolus<br />
heterostrophus race T, which produces T-toxin, a determinant <strong>of</strong> high virulence to maize carrying Texas male sterile<br />
cytoplasm. The genetics <strong>of</strong> T-toxin production is complex and the evolutionary origin <strong>of</strong> associated genes is uncertain. It is<br />
known that ability to produce T-toxin requires three genes encoded at two unlinked loci, Tox1A and Tox1B, which map to the<br />
breakpoints <strong>of</strong> a reciprocal translocation. DNA associated with Tox1A and Tox1B sums to about 1.2 Mb <strong>of</strong> A+T rich, repeated<br />
DNA that is not found in less virulent race O or other Cochliobolus species. Here, we describe identification and targeted<br />
deletion <strong>of</strong> six additional genes, three mapping to Tox1A and three to Tox1B. Mutant screens indicate that all six genes are<br />
involved in Ttoxin production and high virulence to maize. The nine known Toxi genes encode two polyketide synthases<br />
(PKS), one decarboxylase, five dehydrogenases, and one unknown protein. Only two have a similar phylogenetic pr<strong>of</strong>ile. To<br />
trace evolutionary history <strong>of</strong> one <strong>of</strong> the core PKS, DNA from more than 100 Dothideomycete species were screened for<br />
homologs. An ortholog (60% identity) was confirmed in Didymella zeae-maydis, which produces PM-toxin, a polyketide <strong>of</strong><br />
similar structure and biological specificity as Ttoxin. Only one additional Dothideomycete species, the dung ascomycete<br />
Delitschia wintert harbored a paralog. The unresolved evolutionary history and distinctive gene signature <strong>of</strong> the PKS (fastevolving,<br />
discontinuous taxonomic distribution) leaves open the question <strong>of</strong> lateral or vertical transmission. © <strong>2010</strong> The<br />
American Phytopathological Society.<br />
No.400<br />
Author (s) : Indhavivadhana, S., Leerasiri, P., Rattanachaiyanont, M., Laiwejpithaya, S., Tanmahasamut, P., Techatraisak, K., Angsuwathana,<br />
S.<br />
Title : Vaginal atrophy and sexual dysfunction in current users <strong>of</strong> systemic postmenopausal hormone therapy<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 6, June <strong>2010</strong>, Pages 667-675<br />
Document Type : Article<br />
Keywords : Current users; Postmenopausal hormone therapy; Sexual dysfunction; Vaginal atrophy<br />
<strong>Abstract</strong> : Objective: To determine the prevalence <strong>of</strong> vaginal atrophy and sexual dysfunction in current users <strong>of</strong> systemic<br />
postmenopausal hormone therapy (pHT). Material and Method: A cross-sectional study was conducted in 97 current users <strong>of</strong><br />
pHT at Siriraj Menopause Clinic from 2005 to 2007. Subjective symptoms <strong>of</strong> vaginal atrophy and sexual dysfunction were<br />
assessed by interviewing. Objective signs <strong>of</strong> vaginal atrophy were assessed using pelvic examination, vaginal pH, and<br />
maturation value (MV). Results: The prevalence <strong>of</strong> vaginal atrophy in current users <strong>of</strong> systemic pHT determining from patient's<br />
symptoms, pelvic examination, vaginal pH, and MV were 44.3%, 15.5%, 21.6% and 8.8%, respectively. The prevalence <strong>of</strong><br />
sexual dysfunction varied from 48.7% to 71.6% depending on types <strong>of</strong> dysfunction. There was poor association between the<br />
subjective symptoms and signs, and the objective indicators <strong>of</strong> vaginal atrophy. Among various regimens <strong>of</strong> pHT, tibolone had<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
the lowest prevalence <strong>of</strong> subjective atrophic symptoms; estrogen-only pHT had the lowest prevalence <strong>of</strong> objective atrophic<br />
signs; and raloxifene had the highest prevalence <strong>of</strong> atrophic symptoms and signs, and sexual dysfunction. There was<br />
statistically significant association between regimens <strong>of</strong> pHT and objective indicators for vaginal atrophy (p = 0.004 for pH, and<br />
0.000 for MV). Conclusion: Current users <strong>of</strong> systemic pHT still have vaginal atrophy and sexual dysfunction which relates to<br />
regimens <strong>of</strong> pHT. The prevalence <strong>of</strong> vaginal atrophy varies depending on the assessment methods. The subjective method<br />
gives higher prevalence than the objective one does. Since the subjective symptoms <strong>of</strong> vaginal atrophy would have more<br />
adverse effect on quality <strong>of</strong> life than the objective signs do, the authors suggest that patients' complaints be used to assess<br />
factors affecting vaginal atrophy in further research.<br />
No.401<br />
Author (s) : Indhavivadhana, S., Wongwananuruk, T., Rattanachaiyanont, M., Techatraisak, K., Leerasiri, P., Tanmahasamut, P., Popijan BNS,<br />
M.<br />
Title : Prevalence <strong>of</strong> metabolic syndrome in reproductive-aged polycystic ovary syndrome thai women<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 6, June <strong>2010</strong>, Pages 653-660<br />
Document Type : Article<br />
Keywords : Metabolic syndrome; Pcos; Polycystic ovary syndrome; Thai<br />
<strong>Abstract</strong> : Objective: To determine the prevalence <strong>of</strong> metabolic syndrome (MS) in reproductive-aged polycystic ovary syndrome (PCOS)<br />
Thai women. Material and Method: A Cross sectional study was done at the Gynecologic Endocrinology Unit, Department <strong>of</strong><br />
Obstetrics and Gynecology, Faculty <strong>of</strong> Medicine Siriraj Hospital <strong>of</strong> 250 PCOS Thai women who were diagnosed using Revised<br />
Rotterdam 2003 criteria, and who did not take medications affecting sex hormones or lipid metabolism, and attended the<br />
Gynecologic Endocrinology Unit between May 2007 and January 2009. Patents were interviewed and examined for weight,<br />
height, waist circumference, and blood pressure. Venous blood sample <strong>of</strong> each patient was drawn after 12-hour fasting.<br />
Prevalence <strong>of</strong> MS determined using the definitions <strong>of</strong> National Cholesterol Education Program Adult Treatment Panel III (NCEP<br />
ATP III), <strong>International</strong> Diabetes Federation (IDF), and National Heart Lung and Blood Institutes/American Heart Association<br />
(NHLBI/ AHA). Results: Mean ± SD <strong>of</strong> age, body mass index (BMI), and waist circumference (WC) were 25.4 ± 5.8 years, 26.2 ±<br />
7.6 kg/M2, and 82.3 ± 16.3 cm, respectively. Prevalence <strong>of</strong> MS by the definitions <strong>of</strong> NCEP ATP III, IDF, and NHLBI/AHA was<br />
18.0%, 21.2%, and 21.2%, respectively. Of non-MS women, > 40% already had one to two criteria <strong>of</strong> IDF definition. Among<br />
MS women, 100% had central obesity, 50.9% had high blood pressure, 28.3% had impaired fasting blood glucose, 62.3% had<br />
hypertriglyceridemia, and 92.5% had high-density lipoprotein cholesterol < 50 mg/dL. The prevalence <strong>of</strong> MS increased from<br />
10.3% in women aged < 20 years to 50.0% in those aged ≥ 40 years (p <strong>of</strong> trend = 0.003), and from 0.0% in women with BMI<br />
< 23 kg/M2 to 54.5% in those with BMI ≥ 30 kg/M2 (p <strong>of</strong> trend < 0.001). Conclusion: The prevalence <strong>of</strong> MS in<br />
reproductive-aged PCOS Thai women was 18.0% by NCEP ATP III and 21.2% by IDF and NHLBI/AHA. The prevalence varies<br />
only little with definitions <strong>of</strong> diagnostic criteria. The prevalence increases with age and body mass index. Slightly more than<br />
40% <strong>of</strong> the non-MS PCOS Thai women already had one to two criteria <strong>of</strong> MS.<br />
No.402<br />
Author (s) : Indrawattana, N., Sookrung, N., Kulkeaw, K., Seesuay, W., Kongngoen, T., Chongsa-nguan, M., Tungtrongchitr, A., Chaicumpa, W.<br />
Title : Human monoclonal ScFv that inhibits cellular entry and metalloprotease activity <strong>of</strong> tetanus neurotoxin<br />
Source : Asian Pacific Journal <strong>of</strong> Allergy and Immunology, Volume 28, Issue 1, March <strong>2010</strong>, Pages 85-93<br />
Document Type : Article<br />
Keywords : epitope, metalloproteinase, neurotoxin, recombinant protein, retinoic acid, single chain fragment variable<br />
antibody, synaptobrevin, tetanus toxin, tetanus toxoid, unclassified drug, zinc metalloproteinase; antibody production, antigen<br />
binding, article, biopanning, cell permeabilization, controlled study, enzyme activity, enzyme inhibition, Escherichia<br />
coli, human, human cell, molecular cloning, neuroblastoma cell, nonhuman, phage display, phagemid, protein cleavage;<br />
Antibodies, Monoclonal, Cell Line, Tumor, Clostridium tetani, Humans, Immunization,<br />
Passive, Metalloendopeptidases, Metalloproteases, Neurons, Peptide Library, Protein Binding, Protein Engineering, R-SNARE<br />
Proteins, Receptors, Cell Surface, Single-Chain Antibodies, Tetanus, Tetanus Toxin<br />
<strong>Abstract</strong> : Tetanus is a deadly disease <strong>of</strong> warm blooded animals and humans caused by an exotoxin called tetanospasmin or tetanus<br />
neurotoxin (TeNT) produced by anaerobic bacterium named Clostridium tetani. TeNT is an A-B toxin; each molecule consists<br />
<strong>of</strong> a heavy chain (HC) containing cellular receptor binding domain and a light chain (LC) with zinc metalloprotease activity.<br />
TeNT produced in the infected tissue by the bacteria grown under anaerobic condition binds to ganglioside receptors <strong>of</strong><br />
peripheral nerve, and endocytosed. The A subunit exits from the endosome and undergoes a retrograde transport via the<br />
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nerve axon to the spinal cord. This highly toxic enzyme specifically cleaves one <strong>of</strong> the nerve cell SNARE proteins, i.e.,<br />
synaptobrevin, resulting in inhibition <strong>of</strong> the release <strong>of</strong> neurotransmitters (glycine and GABA) from inhibitory interneuron<br />
causing spastic paralysis, the characteristic <strong>of</strong> tetanus. Current treatment mainstay <strong>of</strong> human tetanus is by passively<br />
administering anti-tetanus toxin produced from animals immunized with adjuvanted tetanus toxoid (TT). There are several<br />
obstacles in production and use <strong>of</strong> the animal derived therapeutic antibody especially the allergic reaction and serum<br />
sickness induced by the host immune response to the foreign protein. The animal antibody, mainly IgG, blocks nerve cell<br />
entry <strong>of</strong> the TeNT but does not neutralize the TeNT protease activity per se and cannot reverse the tetanus symptoms. In<br />
this study, fully human single chain antibody fragments (HuScFv) were produced from a human antibody phage display<br />
library. TT was used as antigen in a single round phage bio-panning to select phage clones that display TT bound-HuScFv<br />
from the library. HuScFv from 4 selected huscfv-phagemid transformed E. coli clones inhibited binding <strong>of</strong> the native TeNT to<br />
retinoic acid pulsed human neuroblastoma cells when used at the molecular TeNT:HuScFv ratio <strong>of</strong> 1:100. HuScFv from one<br />
<strong>of</strong> the 4 clones also inhibited the TeNT mediated cleavage <strong>of</strong> recombinant synaptobrevin. Further investigation is needed for<br />
identification <strong>of</strong> epitope specificity <strong>of</strong> these HuScFv and HuScFv effector mechanisms towards the TeNT. Cell penetrating<br />
version <strong>of</strong> the HuScFv that inhibited the TeNT zinc metalloprotease activity should be made. The HuScFv produced in this<br />
study either singly or in their suitable combination warrant developing further to a real use in humans as a surrogate <strong>of</strong> the<br />
animal antibody for treatment <strong>of</strong> tetanus.<br />
No.403<br />
Author (s) : Ingsathit, A., Avihingsanon, Y., Rattanasiri, S., Premasathian, N., Pongskul, C., Jittikanont, S., Sumethkul, V.<br />
Title : Different etiologies <strong>of</strong> graft loss and death in Asian kidney transplant recipients: A report from Thai transplant registry<br />
Source : Transplantation Proceedings, Volume 42, Issue 10, December <strong>2010</strong>, Pages 4014-4016<br />
Document Type : Conference Paper<br />
Keywords : alemtuzumab, cyclosporin, interleukin 2 receptor antibody, mammalian target <strong>of</strong> rapamycin inhibitor, OKT<br />
3, tacrolimus, thymocyte antibody; adult, Asian, cause <strong>of</strong> death, chronic allograft nephropathy, chronic<br />
glomerulonephritis, clinical evaluation, conference paper, data base, death, evaluation and follow up, female, graft<br />
failure, graft recipient, graft rejection, graft survival, heart death, human, hypertensive nephropathy, immunosuppressive<br />
treatment, kidney disease, kidney failure, kidney graft, kidney graft rejection, liver disease, lung infection, major clinical<br />
study, male, priority journal, renal replacement therapy, retransplantation, septicemia, survival<br />
<strong>Abstract</strong> : Background Kidney transplantation is the most performed solid organ transplantation in Thailand. Over 4000 patients have<br />
received kidney transplantation from 23 centers within the kingdom. This study sought to demonstrate the causes <strong>of</strong> graft<br />
loss and death in Thai patients receiving kidney transplant during the past decade. Patients and Methods The Thai Transplant<br />
Registry database was used to evaluate the causes <strong>of</strong> graft loss and death. This database was established since 1997, a total<br />
<strong>of</strong> 2298 kidney transplants were available for analysis. Graft loss was defined as return to dialysis, graft removal,<br />
retransplantation, or death <strong>of</strong> the recipients. Patient survival was analyzed by all deaths. Results Among 2298 recipients, 59%<br />
received organs from deceased donors. The mean age at transplantation was 42 years (SD 12) and 61% were male. The most<br />
common identified causes <strong>of</strong> the end-stage renal disease were chronic glomerulonephritis (25.3%) and hypertensive<br />
nephropathy (11.3%); half <strong>of</strong> those were unknown. Actuarial graft survival rates at 1 and 5 years were 89% and 73%,<br />
respectively. The common causes <strong>of</strong> graft loss were chronic allograft nephropathy (53%), acute rejection (15%), death with a<br />
functioning graft (15%), and transplant renal artery diseases (7%). The greatest proportion (64%) <strong>of</strong> deaths was infection owing<br />
to septicemia and/or pulmonary infection. The others were from cardiovascular deaths (12%), liver disease (6%), and<br />
malignancy (4%). Conclusion Graft survival rates were comparable with previous reports. However, the proportion <strong>of</strong> death<br />
with functioning graft and cardiovascular death as a cause <strong>of</strong> graft and patient loss is lower than that <strong>of</strong> Caucasian<br />
populations. © <strong>2010</strong> Elsevier Inc. All rights reserved.<br />
No.404<br />
Author (s) : Ingsathit, A., Thakkinstian, A., Chaiprasert, A., Sangthawan, P., Gojaseni, P., Kiattisunthorn, K., Ongaiyooth, L., Vanavanan, S.,<br />
Sirivongs, D., Thirakhupt, P., Mittal, B., Singh, A.K.<br />
Title : Prevalence and risk factors <strong>of</strong> chronic kidney disease in the Thai adult population: Thai SEEK study<br />
Source : Nephrology Dialysis Transplantation, Volume 25, Issue 5, May <strong>2010</strong>, Pages 1567-1575<br />
Document Type : Article<br />
Keywords : Chronic kidney disease; Population-based; Prevalence; Risk factor; Stratified-cluster sampling<br />
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<strong>Abstract</strong> : Background. Previous reports <strong>of</strong> chronic kidney disease (CKD) prevalence in Thailand varied from 4.3% to 13.8%. However,<br />
there were methodological concerns with these reports in terms <strong>of</strong> generalization and the accuracy <strong>of</strong> estimation. This study<br />
was, therefore, conducted to determine CKD prevalence and its risk factors in Thai adult populations.Methods. The<br />
population-based Thai Screening and Early Evaluation <strong>of</strong> Kidney Disease (SEEK) study was conducted with cross-sectional<br />
stratified-cluster sampling. Serum creatinine was analysed using the modified Jaffe method and then standardized with<br />
isotope dilution mass spectrometry.Results. The study included 3,459 subjects were included in the study. The mean age was<br />
45.2 years (SE = 0.8), and 54.5% were female. Six hundred and twenty-six subjects were identified as having CKD, which<br />
evidenced an overall CKD prevalence <strong>of</strong> 17.5% [95% confidence interval (95% CI) = 14.6-20.4%]. The CKD prevalence <strong>of</strong><br />
Stages I, II, III and IV were 3.3% (95% CI = 2.5%, 4.1%), 5.6% (95% CI = 4.2%, 7.0%), 7.5% (95% CI = 6.2%, 8.8%) and 1.1% (95%<br />
CI = 0.7%, 1.5%), respectively. The prevalence <strong>of</strong> CKD was higher in Bangkok, the Northern and Northeastern regions than in<br />
the Central and Southern regions. Seven factors (i.e. age, gender, diabetes, hypertension, hyperuricaemia, history <strong>of</strong> kidney<br />
stones and the use <strong>of</strong> traditional medicines) were associated with CKD. Only 1.9% <strong>of</strong> the subjects were aware that they had<br />
CKD.Conclusions. CKD prevalence in the Thai population is much higher than previously known and published. Early stages <strong>of</strong><br />
CKD seem to be as common as later stages. However, albuminuria measurement was not confirmed and adjusting for<br />
persistent positive rates resulted in the prevalence <strong>of</strong> 14.4%. Furthermore, the awareness <strong>of</strong> CKD was quite low in the Thai<br />
population. © The Author 2009. Published by Oxford <strong>University</strong> Press on behalf <strong>of</strong> ERA-EDTA. All rights reserved.<br />
No.405<br />
Author (s) : In-Iw, S., Manaboriboon, B., Chomchai, C.<br />
Title : A comparison <strong>of</strong> body-image perception, health outlook and eating behavior in mildly obese versus moderately-to-severely<br />
obese adolescents<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 4, April <strong>2010</strong>, Pages 429-435<br />
Document Type : Article<br />
Keywords : Adolescent obesity; Body-image; Eating behavior; Health outlook; Social attitude<br />
<strong>Abstract</strong> : Background: Adolescent obesity is rapidly becoming a major pubic health concern in Thailand. Factors that contribute to<br />
adolescent obesity are increasingly sedentary behaviors, changes in eating habits and physical activity. Body-image perception<br />
and peer influence are substantial for adolescents. As a result, knowing the obesity-related psychological impacts in<br />
adolescent will be the great benefit for health care providers in taking care <strong>of</strong> obese adolescents Objective: The purpose <strong>of</strong><br />
this study was to investigate the impact <strong>of</strong> obesity has on the behaviors and attitudes regarding self- perception, health<br />
outlook, and eating behavior in the mildly obese and the moderately-to-severely obese Thai adolescents. Material and<br />
Method: Participants were obese students who were being followed at the school-based Teen Health Clinic on a regular<br />
basis. All participants were followed regularly at their respective school-based Teen Health Clinics. Each participant's percent<br />
weight for height was calculated by using Thai National Growth charts. Participants were categorized into 2 groups according<br />
to the percent weight for height (% WFH). Those with% WFH below 120% was considered not obese and excluded from the<br />
study. The two participating groups were% WFH <strong>of</strong> 120-140% who are mildly obese (M-O group) and% WFH <strong>of</strong> greater than<br />
140% who are moderately-severely obese (M-S-O group). A 3-part questionnaire was used to assess the impact <strong>of</strong> obesity on<br />
emotional and psychosocial aspects <strong>of</strong> each participant, their eating and nutritional attitude and their level <strong>of</strong> physical<br />
activity. Results: Of the 5,366 students, grade 7-12 who attended 2 metropolitan Bangkok schools, 678 were diagnosed as<br />
overweight or obese. There were 175 obese students who attended the clinic regularly were asked to participate in this study<br />
and there were 167 adolescents who completed the questionnaires recruited for this study. Both the M-O and M-S-O groups<br />
reported dissatisfaction with their body weight (85.9% and 91.7% respectively) and perceived their need for weight reduction.<br />
The M-S-O groups expressed more concerns to lose their weight for medical reasons than the M-O group (52.1% vs. 35.2%, pvalue<br />
< 0.03). Being obese was shown to have more significant impact to their confidence in M-S-O group than the M-O group<br />
(71.9% vs. 50.7%, p < 0.005). The students in M-O group eat lunch regularly otherwise M-S-O group, few <strong>of</strong> them, skips lunch<br />
meal (100% vs. 93.8%, p < 0.032). Conclusion: From our study, significant obese adolescents were shown to have poorer selfimage<br />
with greater prevalence being shown in the significantly obese group.<br />
No.406<br />
Author (s) : Inphonlek, S., Pimpha, N., Sunintaboon, P.<br />
Title : Synthesis <strong>of</strong> poly(methyl methacrylate) core/chitosan-mixed-polyethyleneimine shell nanoparticles and their antibacterial<br />
property<br />
Document Type : Article<br />
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Source : Colloids and Surfaces B: Biointerfaces, Volume 77, Issue 2, <strong>2010</strong>, Pages 219-226<br />
Keywords : Antibacterial property; Chitosan; Core-shell nanoparticles; Poly(methyl methacrylate); Polyethyleneimine<br />
<strong>Abstract</strong> : The core-shell nanoparticles possessing poly(methyl methacrylate) (PMMA) core coated with chitosan (CS), polyethyleneimine<br />
(PEI), and chitosan-mixed-polyethyleneimine (CS/PEI) shells were synthesized in this work. The emulsifier-free emulsion<br />
polymerization triggered by a redox initiating system from t-butylhydroperoxide (TBHP) and amine groups on CS and/or PEI<br />
was used as a synthetic method. In the CS/PEI systems, the amount <strong>of</strong> CS was kept constant (0.5. g), while the amount <strong>of</strong> PEI<br />
was varied from 0.1 to 0.5. g. The surface and physico-chemical properties <strong>of</strong> prepared nanoparticles were then examined.<br />
FTIR spectra indicated the presence <strong>of</strong> grafted PMMA on CS and/or PEI, and the weight fraction <strong>of</strong> incorporated PEI in the<br />
CS/PEI nanoparticles. All nanoparticles were spherical in shape with uniform size distribution illustrated by scanning electron<br />
microscopy (SEM). The introduction <strong>of</strong> PEI to CS nanoparticles yielded the higher monomer conversion, grafting efficiency, and<br />
grafting percentage compared with the CS nanoparticles. The size <strong>of</strong> CS/PEI nanoparticles was smaller than the original CS<br />
and PEI nanoparticles, and tended to decrease with increasing amount <strong>of</strong> PEI introduced. The introduction <strong>of</strong> PEI also brought<br />
the higher colloidal stability to the nanoparticles as indicated by zeta-potential measurement and isoelectric point analysis.<br />
The nanoparticles exhibited a promising antibacterial activity against Staphylococcus aureus and Escherichia coli. The<br />
nanoparticle-bacteria interaction was studied via SEM. The results suggested that they would be useful as effective<br />
antibacterial agents. © <strong>2010</strong> Elsevier B.V.<br />
No.407<br />
Author (s) : Insawang, M., Wongpraparut, C.<br />
Title : Recalcitrant solar urticaria induced by UVA and visible light: A case report<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 10, October <strong>2010</strong>, Pages 1238-1241<br />
Document Type : Article<br />
Keywords : Recalcitrant; Solar urticaria; Ultraviolet; UVA; Visible light<br />
<strong>Abstract</strong> : A 41 year-old man presented with a ten-year history <strong>of</strong> recurrent erythema and swelling <strong>of</strong> skin that occurred following sun<br />
exposure even as little as ten minutes. The lesion affected only on the sun exposed area. A phototesting was carried out and<br />
revealed that urticaria was induced following ultraviolet A (UVA) and visible light exposure. Solar urticaria (SU) from UVA and<br />
visible light was diagnosed. Many treatment options including combination <strong>of</strong> oral antihistamines, psoralen plus UVA (PUVA)<br />
photochemotherapy, narrowband UVB (NBUVB, 311 nm) phototherapy, and plasmapheresis were tried in the presented<br />
patient without significant response. Although various treatments are available, managing SU remains a challenging problem in<br />
many patients.<br />
No.408<br />
Author (s) : Intaraprasong, P., Sobhonslidsuk, A., Tongprasert, S.<br />
Title : Donor outcomes after Living Donor Liver Transplantation (LDLT)<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 11, November <strong>2010</strong>, Pages 1340-1343<br />
Document Type : Article<br />
Keywords : Liver transplantation; Living donor; Outcomes<br />
<strong>Abstract</strong> : Liver transplantation is an accepted management for end stage liver disease, early-stage hepatocellular carcinoma, and acute<br />
liver failure. The number <strong>of</strong> patients with end stage liver disease is growing rapidly. Living Donor Liver Transplantation (LDLT)<br />
has become an important alternative to cadaveric organ transplant for patients with end stage liver disease. On average,<br />
about one in three potential donors eventually donate part <strong>of</strong> their liver. The overall reported donor mortality was 0.2% and<br />
median morbidity <strong>of</strong> 16%. Understanding donor outcomes is important as it enables the transplant team to fully inform the<br />
potential donor. In addition, this information will help the transplant team improve their post operative management and<br />
plan for long-term follow-up after liver donation.<br />
No.409<br />
Author (s) : Intasean, N.<br />
Title : MANAGEMENT OF SKIN COMPLICATION AROUND THE STOMA OF OSTOMY PATIENTS<br />
Source : JOURNAL OF WOUND OSTOMY AND CONTINENCE NURSING, Volume: 37, Issue: 3, Supplement: S, Pages: S56-S57, Published:<br />
MAY-JUN <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
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No.410<br />
Author (s) : Inthavilay, S., Franchard, T., Meimei, Y., Ashley, E.A., Barennes, H.<br />
Title : Knowledge and acceptability <strong>of</strong> the rectal treatment route in Laos and its application for pre-referral emergency malaria<br />
treatment<br />
Source : Malaria Journal, Volume 9, Issue 1, <strong>2010</strong>, Article number 342<br />
Document Type : Article<br />
Keywords : artesunate, antimalarial agent, artemisinin derivative; adult, article, constipation, cross-sectional study, emergency<br />
care, family, female, health care personnel, household, human, knowledge, Laos, major clinical<br />
study, malaria, male, nurse, pain, physician, population, rectal drug administration, seizure, urban area, attitude to<br />
health, emergency health service, health personnel attitude, infant, methodology, patient attitude, preschool child, statistics;<br />
Administration, Rectal, Adult, Antimalarials, Artemisinins, Attitude <strong>of</strong> Health Personnel, Child, Preschool, Cross-Sectional<br />
Studies, Emergency Medical Services, Female, Health Knowledge, Attitudes,<br />
Practice, Humans, Infant, Laos, Malaria, Male, Patient Acceptance <strong>of</strong> Health Care<br />
<strong>Abstract</strong> : Background: Rectal artesunate has been shown to reduce death and disability from severe malaria caused by delays in<br />
reaching facilities capable <strong>of</strong> providing appropriate treatment. Acceptability <strong>of</strong> this mode <strong>of</strong> drug delivery in Laos is not<br />
known. In 2009 the acceptability <strong>of</strong> rectal treatments was evaluated among the general Lao population and Lao doctors in a<br />
national survey. Methods. A cross sectional survey was performed <strong>of</strong> 985 households selected through a multi-stage random<br />
sampling process from 85 villages in 12/18 provinces and <strong>of</strong> 315 health staff randomly selected at each administrative level.<br />
Results. Out <strong>of</strong> 985 families, 9% had used the rectal route to treat children (the main indication was seizures or constipation).<br />
The population considered it less effective than other routes. Other concerns raised included pain (28%), discomfort for<br />
children (40%) and the possibility <strong>of</strong> other side effects (20%). Of 300 health staff surveyed (nurses 44%, doctors 66%), only<br />
51% had already used the rectal route with a suppository, mostly to treat fever (76%). Health staff working in provincial<br />
hospitals had more experience <strong>of</strong> using the rectal route than those in urban areas. The majority (92%) were keen to use the<br />
rectal route to treat malaria although oral and intramuscular routes were preferred and considered to be more efficacious.<br />
Discussion and conclusion. Use <strong>of</strong> rectal treatments is uncommon in Laos and generally not considered to be very effective.<br />
This view is shared by the population and health care workers. More information and training are needed to convince the<br />
population and health staff <strong>of</strong> the efficacy and advantages <strong>of</strong> the rectal route for malaria treatment. © <strong>2010</strong> Inthavilay et al;<br />
licensee BioMed Central Ltd.<br />
No.411<br />
Author (s) : Inthira, R., Suparb, AU.<br />
Title : Health status in older patients with chronic low back pain undergoing spinal surgery<br />
Source : JOURNAL OF CLINICAL NURSING, Volume: 19, Supplement: 1, Pages: 84-84, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.412<br />
Author (s) : Inthorn, D., Tipprasertsin, K., Thiravetyan, P., Khan, E.<br />
Title : Color removal from textile wastewater by using treated flute reed in a fixed bed column<br />
Source : Journal <strong>of</strong> Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering, Volume<br />
45, Issue 5, April <strong>2010</strong>, Pages 637-644<br />
Document Type : Article<br />
Keywords : Adsorption; Bed depth service time model; Breakthrough curve; Textile wastewater; Treated flute reed<br />
<strong>Abstract</strong> : This study investigated the ability <strong>of</strong> acid treated flute reed to adsorb color (dye) from synthetic reactive dye solutions, and<br />
actual dyeing and printing textile wastewaters in a laboratory scale fixed bed column. The effects <strong>of</strong> particle size, initial<br />
reactive dye concentration, bed depth and flow rate on adsorption performances were examined. The results from<br />
experiments with synthetic reactive dye solutions showed that the volume treated (until the breakthrough occurred)<br />
increased with decreasing particle size, influent reactive dye concentration and flow rate, and increasing bed depth. The bed<br />
depth service time model was suitable for describing the experimental data. The treated flute reed was able to reduce color<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
efficiently, 99% for dyeing textile wastewater with ten adsorption columns in series and 78% for printing textile wastewater<br />
with a single adsorption column. The difference in the numbers <strong>of</strong> columns used for the two types <strong>of</strong> actual textile<br />
wastewater led to a substantial discrepancy in suspended solids removal, 99% for dyeing wastewater and 12% for printing<br />
wastewater. Similar pH and chemical oxygen demand (COD) results were obtained for the two types <strong>of</strong> textile wastewater.<br />
The acid pretreatment <strong>of</strong> flute reed resulted in dramatic decreases in pH after the adsorption and very acidic effluents (pH 3).<br />
Increases <strong>of</strong> COD after the adsorption due to organic leaching from the treated flute reed were observed. A different<br />
pretreatment method to solve these pH and COD problems is needed before flute reed can be used in practice. Copyright<br />
Copy; Taylor & Francis Group, LLC.<br />
No.413<br />
Author (s) : Isarankura-Na-Ayudhya, C., Nantasenamat, C., Dansethakul, P., Saetum, P., Laosrivijit, S., Prachayasittikul, V.<br />
Title : Solving the barriers to diabetes education through the use <strong>of</strong> multimedia<br />
Source : Nursing and Health Sciences, Volume 12, Issue 1, March <strong>2010</strong>, Pages 58-66<br />
Document Type : Article<br />
Keywords : Action research; Chronic disease; Diabetes mellitus; Health education; Self-awareness; Self-reliance<br />
<strong>Abstract</strong> : Diabetes mellitus is a chronic disease that affects > 180 million people worldwide. It is persistent in Thai communities in spite<br />
<strong>of</strong> much effort in prevention and control. This study examined the knowledge capacity <strong>of</strong> villagers in the Klongmai<br />
community <strong>of</strong> Nakhon Pathom, Thailand, regarding diabetes by way <strong>of</strong> action research. A health status assessment and a<br />
survey <strong>of</strong> the community were carried out and used as the basis for designing an educational video on diabetes that is<br />
accessible regardless <strong>of</strong> age and educational background. Evaluations <strong>of</strong> the pre- and poststudy questionnaires were carried<br />
out using statistical analysis. The results indicated that the devised educational materials were effective in encouraging the<br />
community's self-awareness and perception <strong>of</strong> diabetes at the significance level <strong>of</strong> 0.05. Most importantly, the participants<br />
demonstrated pr<strong>of</strong>iciency in adapting the knowledge gained from the workshop to their own lifestyle. © 2009 Blackwell<br />
Publishing Asia Pty Ltd.<br />
No.414<br />
Author (s) : Isarankura-Na-Ayudhya, C., Tansila, N., Worachartcheewan, A., Bülow, L., Prachayasittikul, V.<br />
Title : Biochemical and cellular investigation <strong>of</strong> vitreoscilla hemoglobin (VHb) variants possessing efficient peroxidase activity<br />
Source : Journal <strong>of</strong> Microbiology and Biotechnology, Volume 20, Issue 3, March <strong>2010</strong>, Pages 532-541<br />
Document Type : Article<br />
Keywords : Catalytic constant; Enzyme kinetics; Iron superoxide dismutase; Peroxidase-like activity; Proteomic analysis; Vitreoscilla<br />
hemoglobin<br />
<strong>Abstract</strong> : Peroxidase-like activity <strong>of</strong> Vitreoscilla hemoglobin (VHb) has been recently disclosed. To maximize such activity, two<br />
catalytically conserved residues (histidine and arginine) found in the distal pocket <strong>of</strong> peroxidases have successfully been<br />
introduced into that <strong>of</strong> the VHb. A 15-fold increase in catalytic constant (kcat) was obtained in P54R variant, which was<br />
presumably attributable to the lower rigidity and higher hydrophilicity <strong>of</strong> the distal cavity arising from substitution <strong>of</strong> proline<br />
to arginine. None <strong>of</strong> the modifications altered the affinity towards either H2O2 or ABTS substrate. Spectroscopic studies<br />
revealed that VHb variants harboring the T29H mutation apparently demonstrated a spectral shift in both ferric and ferrous<br />
forms (406-408 to 411 nm, and 432 to 424-425 nm, respectively). All VHb proteins in the ferrous state had a λsoret peak at<br />
~419 nm following the carbon monoxide (CO) binding. Expression <strong>of</strong> the P54R mutant mediated the downregulation <strong>of</strong> iron<br />
superoxide dismutase (FeSOD) as identified by two-dimensional gel electrophoresis (2-DE) and peptide mass fingerprinting<br />
(PMF). According to the high peroxidase activity <strong>of</strong> P54R, it could effectively eliminate autoxidation-derived H2O2, which is a<br />
cause <strong>of</strong> heme degradation and iron release. This decreased the iron availability and consequently reduced the formation <strong>of</strong><br />
the Fe2+-ferric uptake regulator protein (Fe2+-Fur), an inducer <strong>of</strong> FeSOD expression.<br />
No.415<br />
Author (s) : Isarankura-Na-Ayudhya, C., Tantimongcolwat, T., Galla, H.-J., Prachayasittikul, V.<br />
Title : Fluorescent protein-based optical biosensor for copper ion quantitation<br />
Source : Biological Trace Element Research, Volume 134, Issue 3, June <strong>2010</strong>, Pages 352-363<br />
Document Type : Article<br />
Keywords : Copper ions; Green fluorescent protein; Optical sensor; Sol-gel<br />
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<strong>Abstract</strong> : In the present study, spectroscopic determinations <strong>of</strong> copper ions using chimeric metal-binding green fluorescent protein<br />
(His6GFP) as an active indicator have been explored. Supplementation <strong>of</strong> copper ions to the GFP solution led to a remarkable<br />
decrease <strong>of</strong> fluorescent intensity corresponding to metal concentrations. For circumstances, rapid declining <strong>of</strong> fluorescence<br />
up to 60% was detected in the presence <strong>of</strong> 500 μM copper. This is in contrast to those observed in the case <strong>of</strong> zinc and<br />
calcium ions, in which approximately 10-20% <strong>of</strong> fluorescence was affected. Recovery <strong>of</strong> its original fluorescence up to 80%<br />
was mediated by the addition <strong>of</strong> ethylenediamine tetraacetic acid. More importantly, in the presence <strong>of</strong> metal ions, the<br />
emission wavelength maximum remains unchanged while reduction <strong>of</strong> the optical density <strong>of</strong> the absorption spectrum has<br />
been observed. This indicates that the chromophore's ground state was possibly affected by the static quenching process.<br />
Results from circular dichroism measurements revealed that the overall patterns <strong>of</strong> circular dichroism spectra after exposure<br />
to copper ions were not significantly different from that <strong>of</strong> the control, where the majority <strong>of</strong> sharp positive band around 195-<br />
196 nm in combination with a broad negative deflection around 215-216 nm was obtained. Taken together, it can be<br />
presumed that copper ions exerted their static quenching on the fluorescence rather than structural or conformational<br />
alteration. However, notification has to be made that some peptide rearrangements may also occur in the presence <strong>of</strong> metal<br />
ions. Further studies were conducted to investigate the feasibility <strong>of</strong> using the His6GFP as a sensing unit for copper ions. The<br />
His6GFP was encapsulated in Sol-gel and immobilized onto the optical fiber connected with a fluorescence detecting device.<br />
The Sol-gel was doped into the metal solution where the quenching <strong>of</strong> fluorescence could be monitored in real time. The<br />
sensing unit provided a high sensitivity <strong>of</strong> detection in the range <strong>of</strong> 0.5 μM to 50 mM with high selectivity for copper ions. All<br />
these findings open up a high potential to apply the fluorescent protein-based bioanalytical tool for copper determination in<br />
the future. © 2009 Humana Press Inc.<br />
No.416<br />
Author (s) : Isarankura-Na-Ayudhya, C., Yainoy, S., Tantimongcolwat, T., Bülow, L., Prachayasittikul, V.<br />
Title : Engineering <strong>of</strong> a novel chimera <strong>of</strong> superoxide dismutase and Vitreoscilla Hemoglobin for rapid detoxification <strong>of</strong> reactive<br />
oxygen species<br />
Source : Journal <strong>of</strong> Bioscience and Bioengineering, Volume 110, Issue 6, December <strong>2010</strong>, Pages 633-637<br />
Document Type : Article<br />
Keywords : Bifunctional enzyme; Manganese superoxide dismutase; Reactive oxygen species; Sequential catalytic reactions; Vitreoscilla<br />
hemoglobin<br />
<strong>Abstract</strong> : The genes encoding human manganese superoxide dismutase (MnSOD) and Vitreoscilla hemoglobin (VHb) were fused inframe<br />
to generate a bifunctional enzyme that possessed MnSOD and peroxidase-like activities. At neutral pH, the coupling <strong>of</strong><br />
the SOD and peroxidase reactions revealed that the bifunctional enzyme exhibited a 2.5 times shorter transient period and a<br />
1.67 times higher reaction rate at steady-state conditions. Furthermore, the catalytic rate <strong>of</strong> the bifunctional enzyme was not<br />
affected as much by the external H2O2 scavenger catalase. This indicates that the bifunctional protein possesses a greater<br />
antioxidant capability, which is possibly due to the close proximity between the active site <strong>of</strong> MnSOD and the heme moiety<br />
<strong>of</strong> VHb. Our findings not only provide insight into the synergistic functions <strong>of</strong> SOD and peroxidase but also could potentially<br />
be used to develop novel therapeutic agents with more efficient O2 carrying capability. © <strong>2010</strong> The Society for<br />
Biotechnology, Japan.<br />
No.417<br />
Author (s) : Isarankura-Na-Ayudhya, P., Isarankura-Na-Ayudhya, C., Yainoy, S., Thippakorn, C., Singhagamol, W., Polprachum, W., Roytrakul,<br />
S., Prachayasittikul, V.<br />
Title : Proteomic alterations <strong>of</strong> Escherichia coli by paraquat<br />
Source : EXCLI Journal, Volume 9, <strong>2010</strong>, Pages 108-118<br />
Document Type : Article<br />
Keywords : Escherichia coli; Oxidative stress; Proteomic; Superoxide dismutase; Two-dimensional gel electrophoresis<br />
<strong>Abstract</strong> : Paraquat (PQ; a widely used herbicide) exerts its harmful effect to human, mammals and microorganisms upon intracellular<br />
conversion to superoxide radical. Cellular responses against toxic paraquat remain not fully understood, especially on the<br />
adaptive metabolic changes as a consequence <strong>of</strong> oxidative burden. In this study, alterations <strong>of</strong> metabolic processes <strong>of</strong><br />
Escherichia coli (E. coli) by paraquat were systematically investigated by two-dimensional gel electrophoresis (2-DE) in<br />
conjunction with peptide mass fingerprinting (PMF). In host cells, the first line mechanism was scrutinized by a remarkable<br />
induction <strong>of</strong> endogenous superoxide dismutase (E. coli SOD). The second line involved in the metabolic adaptation and<br />
compensation for energy production by up-or down-regulation <strong>of</strong> the enzymes implicated in glycolysis and tricarboxylic acid<br />
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cycle. Notably, down-regulation <strong>of</strong> aconitase enzyme and changes <strong>of</strong> enzyme is<strong>of</strong>orm from the acidic (pI~5.29) to the higher<br />
basidic form (pI~5.59) were detected. Meanwhile, up-regulation <strong>of</strong> fumarase approximately 4-5 folds were observed.<br />
Importantly, overexpression <strong>of</strong> human manganese superoxide dismutase (human Mn-SOD) in E. coli cells could in turn downregulate<br />
the expression <strong>of</strong> fumarase enzyme. This observation was not found when the cells expressing human catalase were<br />
tested. Other mechanisms such as changes <strong>of</strong> purine nucleoside phosphorylase and protein transporters (D-ribose-binding<br />
protein and oligopeptide binding protein) were also accounted. However, among all the differentially expressed proteins, the<br />
fumarase enzyme is evidenced to be a major target responsible for superoxide-generating paraquat, which may further be<br />
applied as a potential biomarker for paraquat toxicity in the future.<br />
No.418<br />
Author (s) : Itthipanichpong, R., Lupreechaset, A., Chotewuttakorn, S., Akarasereenont, P., Onkoksoong, T., Palo, T., Kongpatanakul, S.,<br />
Chatsiricharoenkul, S., Thitilertdecha, P., Punpeng, P., Laohapand, T.<br />
Title : Effect <strong>of</strong> Ayurved Siriraj Herbal recipe Chantaleela on platelet aggregation<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 1, January <strong>2010</strong>, Pages 115-122<br />
Document Type : Article<br />
Keywords : ADP; Adrenaline; Platelet aggregation; Siriraj Ayurved recipe<br />
<strong>Abstract</strong> : Introduction: Ayurved Siriraj Chantaleela recipe is a traditional Thai remedy consisting <strong>of</strong> eight medicinal plants, which is<br />
employed for the treatment <strong>of</strong> fever. Objective: To investigate the effects <strong>of</strong> Ayurved Siriraj Herbal recipe Chantaleela on<br />
platelet aggregation. Study design: Clinical research; ex vivo with before and after study design. Material and Method: Twelve<br />
healthy male and female volunteers participated in the present study. Platelet aggregation test before Chantaleela ingestion<br />
was done as a control. After administration <strong>of</strong> 750 mg Chantaleela (3 x 250 mg tablets) every 8 hours for 3 doses, platelet<br />
aggregation was measured 8 hours following the first dose using an aggregometer and microplate reader. Adrenaline (Adr) and<br />
adenosine diphosphate (ADP) were used as platelet stimulants. Platelet aggregation was measured again at 32 hours and 8-10<br />
days after the first dose. Results: All <strong>of</strong> the participants completed the present study without any adverse event. Ayurved<br />
Siriraj Chantaleela did not affect platelet aggregation; neither Adr nor ADP were used as platelet agonists in both<br />
aggregometer and microplate reader. Subgroup analysis revealed no significant change in platelet aggregation after<br />
Chantaleela administration according to the control for both male and female groups. The same results were also obtained in<br />
other subgroup analysis including hyperaggregation group, hypo-normal aggregation group. Conclusion: From the present<br />
study, normal dose <strong>of</strong> Chantaleela for alleviation <strong>of</strong> fever does not have an effect on either platelet aggregation or platelet<br />
numbers. It may conclude that the present study supports the safety use <strong>of</strong> Chantaleela for relieving fever, as platelet status<br />
does not need to be taken into consideration.<br />
No.419<br />
Author (s) : Ittichaikulthol, W., Prachanpanich, N., Kositchaiwat, C., Intapan, T.<br />
Title : The post-operative analgesic efficacy <strong>of</strong> celecoxib compared with placebo and parecoxib after total hip or knee arthroplasty<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 8, August <strong>2010</strong>, Pages 937-942<br />
Document Type : Article<br />
Keywords : Celecoxib; Parecoxib; Postoperative pain<br />
<strong>Abstract</strong> : Background: Nonsteroidal antiinflammatory drugs (NSAIDs) in combination with opioids is a model <strong>of</strong> multimodal analgesia.<br />
NSAIDs have the oral and parenteral forms. Objective: The aim <strong>of</strong> the present study was to evaluate the efficacy <strong>of</strong> celecoxib<br />
compared with placebo and parecoxib after total hip or knee arthroplasty. Material and Method: A total <strong>of</strong> 120, ASA 1-2, aged<br />
18-75 years, patients were randomly assigned to receive one <strong>of</strong> the three groups: Group I (control) received placebo (n = 40),<br />
group II received 400 mg celecoxib orally (n = 40) and group III received 40 mg parecoxib intravenously (n = 40). The present<br />
study medication was administered 1 hour before surgery. All patients had access to patient-controlled analgesia (PCA) with<br />
intravenous morphine. Patients were studied at 0,1,6,12 and 24 hours postoperatively for verbal numerical rating scale (VNRS),<br />
morphine consumption,satisfaction score and side effects. Results: The intraoperatively fentayl requirement were similar<br />
among the three groups (p < 0.00). Celecoxib and parecoxib significantly decreased the amount <strong>of</strong> morphine requirement<br />
after total hip or knee arthroplasty compared to placebo at 1, 6, 12 and 24 hours (p < 0.00). The celecoxib group required<br />
more morphine than the parecoxib group at 1, 6, 12 and 24 hours (p < 0.00). The VNRS score in parecoxib group was<br />
significantly lower than the celecoxib and control groups at 1,6,12 but not at 24 hours. The VNRS score was lower in the<br />
celecoxib group compared to the control group at 1 and 6 hours postoperatively (p = 0.01, p < 0.01 respectively). The<br />
placebo group had a higher sedation score (p = 0.008) but not for nausea vomiting (p = 0.36) and pruritus (p = 0.12)<br />
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compared to the treatment groups. Conclusion: Within 12 hours after total hip and knee arthroplasty, pre-operative<br />
administration <strong>of</strong> parenteral parecoxib 40 mg was more effective than oral celecoxib 400 mg and placebo in terms <strong>of</strong><br />
morphine consumption and VNRS score.<br />
No.420<br />
Author (s) : Ittiprasert, W., Miller, A., Myers, J., Nene, V., El-Sayed, N.M., Knight, M.<br />
Title : Identification <strong>of</strong> immediate response genes dominantly expressed in juvenile resistant and susceptible Biomphalaria glabrata<br />
snails upon exposure to Schistosoma mansoni<br />
Source : Molecular and Biochemical Parasitology, Volume 169, Issue 1, January <strong>2010</strong>, Pages 27-39<br />
Document Type : Article<br />
Keywords : Differential gene-regulation; Juvenile Biomphalaria glabrata; Resistant; Schistosoma mansoni; Suppression subtractive<br />
hybridization (SSH); Susceptible<br />
<strong>Abstract</strong> : Resistance or susceptibility <strong>of</strong> the snail host Biomphalaria glabrata to Schistosoma mansoni is determined by the genetics <strong>of</strong><br />
both the snail and parasite. Although Mendelian genetics governs adult resistance to infection, juvenile resistance and<br />
susceptibility are complex traits. In this study, suppression subtractive hybridization was used to construct forward and<br />
reverse cDNA libraries to identify genes involved in the immediate response <strong>of</strong> juvenile resistant (BS-90), non-susceptible<br />
(LAC2) snails, and susceptible (NMRI) snails after early exposure to S. mansoni. Expressed Sequence Tags (ESTs) were<br />
generated from the repertoire <strong>of</strong> enriched transcripts. In resistant snails, several ESTs corresponded to transcripts involved in<br />
immune regulation/defense response. While no defense related transcripts were found among juvenile susceptible snail ESTs,<br />
we detected transcripts involved in negative regulation <strong>of</strong> biological process/morphogenesis/proliferation. Differential gene<br />
expression and temporal regulation <strong>of</strong> representative transcripts were compared among snails pre- and post-exposure to<br />
either normal or attenuated miracidia using quantitative real time RT-PCR. Results showed that several transcripts, such as<br />
fibrinolytic C terminal domain, cytidine deaminase, macrophage expressed gene 1, protein kinase C receptor, anti-microbial<br />
peptide; theromacin and Fas remained up-regulated regardless <strong>of</strong> whether or not snails were exposed to normal or<br />
attenuated miracidia. While ESTs related to C-type lectin and low-density lipoprotein receptor were induced only by<br />
exposure to normal miracidia. By comparing changes in gene expression between resistant and susceptible juvenile snails<br />
responding either to normal or attenuated parasites, we can conclude that the transcription <strong>of</strong> genes associated with the<br />
intra-dermal penetration process <strong>of</strong> the snail host by invading miracidia may need to be taken into account when assessing<br />
differential gene expression between resistant and susceptible strains <strong>of</strong> B. glabrata in relation to S. mansoni exposure. ©<br />
2009 Elsevier B.V. All rights reserved.<br />
No.421<br />
Author (s) : Iwagami, M., Susomboon, P., Tangpukdee, N., Wilairatana, P., Krudsood, S., Dao, L.D., Nakazawa, S., Sinuon, M., Socheat, D.,<br />
Yasuoka, J., Jimba, M., Kano, S.<br />
Title : ANALYSES OF PLASMODIUM FALCIPARUM CHLOROQUINE RESISTANCE TRANSPORTER GENE (PFCRT) AND MICROSATELLITE DNA<br />
LOCI FLANKING THE GENE REVEALED GEOGRAPHICALLY DIFFERENT DISSEMINATION OF CHLOROQUINE RESISTANT MALARIA IN<br />
SOUTHEAST ASIA<br />
Source : AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, Volume: 83, Issue: 5, Supplement: S, Pages: 378-378, Meeting<br />
<strong>Abstract</strong>: 1271, Published: NOV <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.422<br />
Author (s) : Izzard, L., Fuller, A., Blacksell, S.D., Paris, D.H., Richards, A.L., Aukkanit, N., Nguyen, C., Jiang, J., Fenwick, S., Day, N.P.J., Graves,<br />
S., Stenos, J.<br />
Title : Isolation <strong>of</strong> a novel Orientia species (O. chuto sp. nov.) from a patient infected in Dubai<br />
Source : Journal <strong>of</strong> Clinical Microbiology, Volume 48, Issue 12, December <strong>2010</strong>, Pages 4404-4409<br />
Document Type : Article<br />
Keywords : RNA 16S; adult, antibody titer, article, bacterium culture, bacterium isolation, blood culture, case report, controlled<br />
study, Dubai, female, gene sequence, human, nucleotide sequence, open reading frame, Orientia, Orientia chuto, Orientia<br />
tsutsugamushi, priority journal, Rickettsiaceae infection, scrub typhus, species comparison, species diversity, strain difference;<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Antibodies, Bacterial, Australia, Bacterial Proteins, Blood, Cluster Analysis, DNA, Bacterial, DNA,<br />
Ribosomal, Female, Humans, Middle Aged, Molecular Sequence Data, Phylogeny, Rickettsieae, RNA, Ribosomal, 16S, Scrub<br />
Typhus, Sequence Analysis, DNA, Travel, United Arab Emirates; Orientia, Orientia tsutsugamushi<br />
<strong>Abstract</strong> : In July 2006, an Australian tourist returning from Dubai, in the United Arab Emirates (UAE), developed acute scrub typhus. Her<br />
signs and symptoms included fever, myalgia, headache, rash, and eschar. Orientia tsutsugamushi serology demonstrated a 4fold<br />
rise in antibody titers in paired serum collections (1:512 to 1:8,192), with the sera reacting strongest against the Gilliam<br />
strain antigen. An Orientia species was isolated by the in vitro culture <strong>of</strong> the patient's acute blood taken prior to antibiotic<br />
treatment. The gene sequencing <strong>of</strong> the 16S rRNA gene (rrs), partial 56-kDa gene, and the full open reading frame 47-kDa gene<br />
was performed, and comparisons <strong>of</strong> this new Orientia sp. isolate to previously characterized strains demonstrated significant<br />
sequence diversity. The closest homology to the rrs sequence <strong>of</strong> the new Orientia sp. isolate was with three strains <strong>of</strong> O.<br />
tsutsugamushi (Ikeda, Kato, and Karp), with a nucleotide sequence similarity <strong>of</strong> 98.5%. The closest homology to the 47-kDa<br />
gene sequence was with O. tsutsugamushi strain Gilliam, with a nucleotide similarity <strong>of</strong> 82.3%, while the closest homology to<br />
the 56-kDa gene sequence was with O. tsutsugamushi strain TA686, with a nucleotide similarity <strong>of</strong> 53.1%. The molecular<br />
divergence and geographically unique origin lead us to believe that this organism should be considered a novel species.<br />
Therefore, we have proposed the name "Orientia chuto," and the prototype strain <strong>of</strong> this species is strain Dubai, named after<br />
the location in which the patient was infected. Copyright © <strong>2010</strong>, American Society for Microbiology. All Rights Reserved.<br />
No.423<br />
Author (s) : Jaichumjai, P., Valyasevi, R., Assavanig, A., Kurdi, P.<br />
Title : Isolation and characterization <strong>of</strong> acid-sensitive Lactobacillus plantarum with application as starter culture for Nham<br />
production<br />
Source : Food Microbiology, Volume 27, Issue 6, September <strong>2010</strong>, Pages 741-748<br />
Document Type : Article<br />
Keywords : Acid-sensitive; H+-ATPase activity; Internal pH; Lactobacillus plantarum<br />
<strong>Abstract</strong> : The aim <strong>of</strong> this study was to derive new starter culture variants that are unable to grow below pH 4.6, the desirable pH <strong>of</strong> the<br />
Thai fermented pork sausage, Nham, specified by Thailand Food Standard, and apply them in Nham fermentation. Several<br />
acid-sensitive mutants <strong>of</strong> one <strong>of</strong> the commercial Nham starter cultures, Lactobacillus plantarum BCC 9546, were isolated as<br />
spontaneous neomycin-resistant mutants. The growth <strong>of</strong> three representative mutants was characterized in MRS broth, which<br />
revealed that their cell numbers and acid production were lower than that <strong>of</strong> the wild-type. The H+-ATPase activities <strong>of</strong> the<br />
three mutants were found significantly lower than that <strong>of</strong> the wild-type under either neutral or acidic conditions.<br />
Consequently, internal pH values <strong>of</strong> the mutants appeared to be lower, especially in acidic environment (pH 5). The most<br />
acid-sensitive mutant was applied in experimental Nham production and the pH <strong>of</strong> Nham fermented with the mutant had<br />
significantly higher pH at the end <strong>of</strong> fermentation (3 days) and after an additional 4 days <strong>of</strong> storage at 30°C. These results<br />
indicate that the use <strong>of</strong> acid-sensitive L. plantarum as starter culture can reduce the severity <strong>of</strong> post-acidification and increase<br />
the shelf life <strong>of</strong> Nham at ambient temperature. © <strong>2010</strong> Elsevier Ltd.<br />
No.424<br />
Author (s) : Jaijoy, K., Soonthornchareonnon, N., Lertprasertsuke, N., Panthong, A., Sireeratawong, S.<br />
Title : Acute and chronic oral toxicity <strong>of</strong> standardized water extract from the fruit <strong>of</strong> Phyllanthus emblica Linn<br />
Source : <strong>International</strong> Journal <strong>of</strong> Applied Research in Natural Products, Volume 3, Issue 1, <strong>2010</strong>, Pages 48-58<br />
Document Type : Article<br />
Keywords : Acute toxicity; Chronic toxicity; Phyllanthus emblica Linn.<br />
<strong>Abstract</strong> : Phyllanthus emblica Linn. (Euphorbiaceae) is an herbal plant commonly used in Asian traditional medicine systems for<br />
treatment <strong>of</strong> many disorders. In the present study, we investigated for the first time acute and chronic toxicity <strong>of</strong> the<br />
standardized water extract <strong>of</strong> P. emblica fruit. The water extract <strong>of</strong> P. emblica was prepared according to the Thai Herbal<br />
Pharmacopoeia and standardized to 20% gallic acid. For studying acute toxicity study, single oral dose <strong>of</strong> 5000 mg water<br />
extract /kg body weight was administered to Sprague Dawley rats (five females, five males). The results showed no toxicity in<br />
terms <strong>of</strong> general behavior change, mortality, or change in gross appearance <strong>of</strong> internal organs (LD50 > 5,000 mg/kg). Chronic<br />
toxicity was studied by daily oral dose (ten females, ten males) <strong>of</strong> 300, 600 and 1,200 mg/kg for 270 days. The results showed<br />
slightly significant differences in the body and organ weights between the control and treatment groups. In addition, the rats<br />
were analyzed for final body and organ weights, necropsy, and hematological, blood chemical and histopathological<br />
parameters. Hematological analysis and clinical blood chemistry revealed slightly changes, but were within the normal limits.<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No gross or histopathology findings were observed in the treatment groups. Altogether, the standardized water extract from<br />
the fruit <strong>of</strong> P. emblica did not produce acute and chronic toxicity in its traditional uses. Industrial relevance: Traditional<br />
medicine is still stronghold <strong>of</strong> more than 50% <strong>of</strong> the world population, especially in developing countries. Nowadays, a<br />
number <strong>of</strong> medicinal plants (such as Phyllanthus emblica Linn.) are commercially available on local markets as a tonic, food<br />
and/or dietary supplement product commonly used for clinical management <strong>of</strong> several conditions. Despite the widespread<br />
use <strong>of</strong> this plant, there is still little literature on the scientific evaluation <strong>of</strong> their toxicity. Results <strong>of</strong> the current study provide<br />
pivotal evidences for ascertaining the safety <strong>of</strong> the stardardized water extract <strong>of</strong> P. emblica fruit that could be used as food<br />
supplements, dietary supplements, etc. in folkloric medicine. © <strong>2010</strong> Healthy Synergies <strong>Publications</strong>.<br />
No.425<br />
Author (s) : Jaijoy, K., Soonthornchareonnon, N., Panthong, A., Sireeratawong, S.<br />
Title : Anti-inflammatory and analgesic activities <strong>of</strong> the water extract from the fruit <strong>of</strong> Phyllanthus emblica Linn<br />
Source : <strong>International</strong> Journal <strong>of</strong> Applied Research in Natural Products, Volume 3, Issue 2, <strong>2010</strong>, Pages 28-35<br />
Document Type : Article<br />
Keywords : Analgesic activity; Anti-inflammatory activity; Phyllanthus emblica Linn.<br />
<strong>Abstract</strong> : The fresh or dry fruit <strong>of</strong> Phyllanthus emblica Linn. is used in traditional medicines for the treatment <strong>of</strong> diarrhea, jaundice and<br />
inflammatory disorder. In the present study, we investigated the anti-inflammatory and analgesic activities <strong>of</strong> the standardized<br />
water extract from the fruit <strong>of</strong> Phyllanthus emblica was prepared according to the Thai Herbal Pharmacopoeia (THP). P.<br />
emblica water extract was evaluated for its antiinflammatory activity in rats using ethyl phenylpropiolate (EPP)-induced and<br />
arachidonic acid (AA)-induced ear edema, carrageenan-induced paw edema as well as cotton pellet-induced granuloma<br />
models, and its analgesic activity in mice using formalin test. The extract at 1 mg/ear exhibited anti-inflammatory effect on<br />
EPP-induced ear edema, but not on AA-induced ear edema. Oral administration <strong>of</strong> P. emblica at the doses <strong>of</strong> 150, 300 and<br />
600 mg/kg caused dose-dependent inhibition <strong>of</strong> carrageenan-induced rat paw edema. P. emblica at 600 mg/kg did reduce<br />
neither transudative and proliferative phases nor body weight gain and thymus weight in cotton pelletinduced granuloma<br />
formation. The extract at the doses <strong>of</strong> 150, 300 and 600 mg/kg elicited a significant analgesic activity in a dose-dependent<br />
manner on both the early and late phase <strong>of</strong> formalin test. The anti-inflammatory and analgesic mechanism <strong>of</strong> activity <strong>of</strong> the<br />
standardized water extract <strong>of</strong> P. emblica seems to be similar to NSAIDs rather than to steroidal drugs. Inhibitory effect on the<br />
synthesis and/or release <strong>of</strong> inflammatory or pain mediators may be the main mechanisms <strong>of</strong> action <strong>of</strong> P. emblica water<br />
extract. Industrial relevance: Medicinal plants have long been recognized as an important source <strong>of</strong> therapeutically effective<br />
treatment for inflammatory diseases. Many patients are turning to herbal medicine as their primary, complementary or<br />
alternative therapies because <strong>of</strong> the adverse effects <strong>of</strong> the pharmaceutical drugs. P. emblica fruit has been used in traditional<br />
management <strong>of</strong> some painful and inflammatory conditions and this has been supported by the present study. This work will<br />
be useful to the industry to produce standardized herbal formulation more effective in the treatment <strong>of</strong> pain and<br />
inflammation with less toxic and less costly than the current synthetic drugs. © <strong>2010</strong> Healthy Synergies <strong>Publications</strong>.<br />
No.426<br />
Author (s) : Jaijoy, K.; Soonthornchareonnon, N.; Panthong, A.; Sireeratawong, S.<br />
Title : Anti-inflammatory activity and toxicity <strong>of</strong> the standardised water extract <strong>of</strong> Phyllanthus emblica L.<br />
Source : PLANTA MEDICA, Volume: 76, Issue: 12, Pages: 1224-1224, Published: AUG <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.427<br />
Author (s) : Jaisin, Y.; Thampithak, A.; Suksamrarn, A.; Phumala-Morales, N.; Govitrapong, P.; Sanvarinda, Y.<br />
Title : PROTECTIVE EFFECT OF CURCUMIN ANALOGUE (COMPOUND 005) AGAINST 6-OHDA INDUCED APOPTOSIS IN SH-SY5Y CELLS<br />
Source : JOURNAL OF NEUROCHEMISTRY, Volume: 115, Supplement: 1, Pages: 54-54, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
157
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.428<br />
Author (s) : Jaisutti, R., Hongkachern, T., Osotchan, T.<br />
Title : Response differences <strong>of</strong> binary vapors from mixtures in liquid and gas phases<br />
Source : Proceedings - 1st <strong>International</strong> Conference on Sensor Device Technologies and Applications, SENSORDEVICES <strong>2010</strong>, <strong>2010</strong>,<br />
Article number 5632178, Pages 249-252<br />
Document Type : Conference Paper<br />
Keywords : Binary mixture; Gas phase; Liquid phase; Principal component analysis; Wavelet transform<br />
<strong>Abstract</strong> : Response differences in binary mixture vapors are investigated by using two systems including the liquid phase and gas phase<br />
mixing systems. The response signal with various mixing ratios are analyzed for methanol-acetone and ethanol-acetone<br />
mixtures in both maximum response and decay characteristic during exposed and recovery periods. Both feature<br />
characteristics can be extracted by discrete wavelet transform. The classification patterns <strong>of</strong> binary mixing vapors are<br />
identifying by principal component analysis. The results show that the mixture points at various ratios are clearly separated<br />
and located far from to the pure acetone vapor point. The relative high vapor pressure <strong>of</strong> acetone plays significant role on<br />
separation effect and also on the different response in liquid and gas phase mixing systems. The main feature <strong>of</strong> these<br />
response differences in binary vapors from mixtures in liquid and gas phase can be extracted from the decay characteristic <strong>of</strong><br />
these various sensor responses. © <strong>2010</strong> IEEE.<br />
No.429<br />
Author (s) : Jaisutti, R., Juagwon, T., Chamlek, O., Osotchan, T.<br />
Title : Property modification <strong>of</strong> copper phthalocyanine film by nitrogen dioxide gas<br />
Source : Advanced Materials Research, Volume 93-94, <strong>2010</strong>, Pages 533-536<br />
Document Type : Conference Paper<br />
Keywords : Copper phthalocyanine; Crystalline structure; Nitrogen dioxide; Surface morphology<br />
<strong>Abstract</strong> : Copper Phthalocyanine (CuPc) thin films have a potential to use as nitrogen dioxide (NO2) gas sensor therefore the study <strong>of</strong><br />
property modification <strong>of</strong> CuPc thin film by NO2 gas can lead to a better understanding the sensor operation. CuPc thin films<br />
were prepared by thermal vacuum deposition on glass substrate and aluminum interdigitated electrodes. The film<br />
characteristics were investigated with and without NO2 gas. These characteristics indicated that the resistance <strong>of</strong> CuPc film<br />
decrease as the NO2 concentration increases. In order to achieve high efficient <strong>of</strong> sensing properties, the post-deposition film<br />
was also annealed. The surface modifications were investigated with different exposed time <strong>of</strong> NO2 gas. Their properties<br />
modifications before and after annealing were investigated on the film morphology and crystalline structure. The surface<br />
morphology <strong>of</strong> the film was examined by atomic force microscopy while the crystalline structure <strong>of</strong> the film was determined<br />
by x-ray diffraction. The electrical measurements <strong>of</strong> CuPc film exposed to NO2 gas were studied in order to investigate<br />
property modification <strong>of</strong> the CuPc film. © (<strong>2010</strong>) Trans Tech <strong>Publications</strong>.<br />
No.430<br />
Author (s) : Jaisutti, R., Yamwong, W., Atthi, N., Pratontep, S., Osotchan, T.<br />
Title : Organic thickness dependence <strong>of</strong> organic field-effect transistor devices based on pentacene<br />
Source : INEC <strong>2010</strong> - <strong>2010</strong> 3rd <strong>International</strong> Nanoelectronics Conference, Proceedings, <strong>2010</strong>, Article number 5424619, Pages 187-188<br />
Document Type : Conference Paper<br />
Keywords : 2-D numerical simulation, Active Layer, Bottom gate, Channel length, Conducting channels, Conducting layers, Drain<br />
electrodes, Electrical characteristic, Electrical property, Gate voltages, Pentacene film, Pentacenes, Saturated<br />
current, Thickness dependence, Thickness layers, Top contact structures; Computer simulation, Electric properties, Field<br />
effect transistors, Nanoelectronics, Semiconducting organic compounds; Organic field effect transistors<br />
<strong>Abstract</strong> : The electrical properties <strong>of</strong> OFET devices were studied as a function <strong>of</strong> the thickness <strong>of</strong> pentacene active layer. A bottom<br />
gate, top contact structure was investigated by measuring the electrical characteristics with 100 μm channel length and<br />
comparing with the results <strong>of</strong> 2-D numerical simulation at various organic thickness layers. The saturated current at same gate<br />
voltage was increased when the pentacene film was thicker. From the simulation cross-section results, this is due to the<br />
carrier has to move from source to drain electrodes through the conducting channel and across the whole thick <strong>of</strong> active<br />
layer. However, these increasing currents are not only depending on conducting layer but also strongly depending on the<br />
increasing <strong>of</strong> threshold voltage when organic thickness is increased. ©<strong>2010</strong> IEEE.<br />
158
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.431<br />
Author (s) : Jangiam, W., Loprasert, S., Smith, D.R., Tungpradabkul, S.<br />
Title : Burkholderia pseudomallei Rpos regulates OxyR and the katG-dpsA operon under conditions <strong>of</strong> oxidative stress<br />
Source : Microbiology and Immunology, Volume 54, Issue 7, July <strong>2010</strong>, Pages 389-397<br />
Document Type : Article<br />
Keywords : Burkholderia pseudomallei; Oxidative stress; OxyR; RpoS<br />
<strong>Abstract</strong> : Burkholderia pseudomallei, the causative agent <strong>of</strong> the potentially fatal tropical disease melioidosis, is known to be highly<br />
resistant to oxidative stress although the mechanism <strong>of</strong> this resistance remains to be fully elucidated. Previous studies have<br />
shown that an OxyR is involved in the regulation <strong>of</strong> oxidative stress via the katG and dpsA genes encoding KatG and DpsA and<br />
that the alternative sigma factor, RpoS, plays a critical role in resistance to oxidative stress by regulating katG and katE genes.<br />
Here it is shown that RpoS is essential for expression <strong>of</strong> the oxidative stress regulator OxyR, since a mutant strain lacking RpoS<br />
failed to induce oxyR expression both during normal growth and under conditions <strong>of</strong> oxidative stress. It is further<br />
demonstrated that the RpoS acts as a positive transcriptional regulator <strong>of</strong> oxyR and dpsA expression, while OxyR acts as a<br />
negative transcriptional regulator <strong>of</strong> the katG-dpsA operon via OxyR repressor under normal growth conditions, and as a<br />
positive transcriptional regulator via OxyR under conditions <strong>of</strong> oxidative stress. Therefore both RpoS and OxyR are required to<br />
promote expression <strong>of</strong> both the katG-dpsA operon and dpsA gene. © <strong>2010</strong> The Societies and Blackwell Publishing Asia Pty<br />
Ltd.<br />
No.432<br />
Author (s) : Jantaratnotai, N.; Schwab, C.; Ryu, J.K.; McGeer, P.L.; McLarnon, J.G.<br />
Title : Converging Perturbed Microvasculature and Microglial Clusters Characterize Alzheimer Disease Brain<br />
Source : CURRENT ALZHEIMER RESEARCH, Volume: 7, Issue: 7, Pages: 625-636, DOI: 10.2174/156720510793499039, Published: NOV <strong>2010</strong><br />
Document Type : Article<br />
Keywords : Alzheimer disease; microglial clusters; perturbed angiogenesis; extravascular laminin and fibrinogen; TNF-alpha; VEGF; leaky<br />
blood brain barrier<br />
<strong>Abstract</strong> : We have investigated physical properties <strong>of</strong> microvasculature and vessel association with microglial clusters in cortical tissue<br />
from Alzheimer disease individuals, classified as severe (ADsev) or mild (ADmild), and nondemented controls (ND).<br />
Immunostaining with laminin or von Willebrand factor demonstrated numbers <strong>of</strong> microvessels and microvascular density<br />
were significantly higher in ADsev cases compared with levels in ADmild or ND cases suggesting proangiogenic activity in ADsev<br />
brain. Evidence for extravascular laminin immunoreactivity was found in ADsev tissue and was largely absent in ADmild and<br />
ND cases suggesting vascular remodeling in ADsev brain included abnormalities in blood vessels. Microgliosis was progressively<br />
increased from ND to ADmild to ADsev with the latter demonstrating areas <strong>of</strong> clustered microglia (groupings <strong>of</strong> three or more<br />
cells) rarely observed in ADmild or ND cases. Microglial clusters in ADsev brain were in close proximity with extravascular<br />
laminin and also plasma protein, fibrinogen, implicating vascular perturbation as a component <strong>of</strong> inflammatory reactivity.<br />
ADsev brain also exhibited elevated levels <strong>of</strong> the proinflammatory/angiogenic factors tumor necrosis factor-alpha (TNF-alpha)<br />
and vascular endothelial growth factor (VEGF) in association, relative to non-association, with microglial clusters. The presence<br />
<strong>of</strong> extravascular laminin and fibrinogen and the vascular modifying factors, TNF-alpha and VEGF in localization with clusters <strong>of</strong><br />
activated microglia, is consistent with microglial-induced vascular remodeling in ADsev brain. Microglial-vascular reciprocal<br />
interactions could serve a critical role in the amplification and perpetuation <strong>of</strong> inflammatory reactivity in AD brain.<br />
No.433<br />
Author (s) : Janvilisri, T., Scaria, J., Chang, Y.-F.<br />
Title : Transcriptional pr<strong>of</strong>iling <strong>of</strong> Clostridium difficile and Caco-2 cells during infection<br />
Source : Journal <strong>of</strong> Infectious Diseases, Volume 202, Issue 2, 15 July <strong>2010</strong>, Pages 282-290<br />
Document Type : Article<br />
Keywords : transcriptome; article, cell communication, cell metabolism, cell strain CACO 2, cell transport, cell viability, Clostridium<br />
difficile, Clostridium difficile infection, controlled study, gene expression, gene expression pr<strong>of</strong>iling, gene expression<br />
regulation, human, human cell, microarray analysis, nonhuman, priority journal, protein protein interaction, reverse<br />
transcription polymerase chain reaction, RNA isolation, signal transduction, transcription regulation, cell survival, colon<br />
tumor, DNA microarray, genetic transcription, genetics, in situ<br />
hybridization, microbiology, pathogenicity, pathology, physiology, pseudomembranous colitis; Caco-2 Cells, Cell<br />
Survival, Clostridium difficile, Colonic Neoplasms, Enterocolitis, Pseudomembranous, Gene Expression Pr<strong>of</strong>iling, Gene<br />
159
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Expression Regulation, Bacterial, Humans, In Situ Hybridization, Oligonucleotide Array Sequence Analysis, Reverse Transcriptase<br />
Polymerase Chain Reaction, Transcription, Genetic<br />
<strong>Abstract</strong> : Clostridium difficile is well recognized as the most common infectious cause <strong>of</strong> nosocomial diarrhea. The incidence and<br />
severity <strong>of</strong> C. difficile infection (CDI) is increasing worldwide. Here, we evaluated simultaneously the transcriptional changes in<br />
the human colorectal epithelial Caco-2 cells and in C. difficile after infection. A total <strong>of</strong> 271 transcripts in Caco-2 cells and 207<br />
transcripts in C. difficile were significantly differentially expressed at ≥1 time point during CDI. We used the gene ontology<br />
annotations and protein-protein network interactions to underline a framework <strong>of</strong> target molecules that could potentially<br />
play a key role during CDI. These genes included those associated with cellular metabolism, transcription, transport, cell<br />
communication, and signal transduction. Our data identified certain key factors that have previously been reported to be<br />
involved in CDI, as well as novel determinants that may participate in a complex mechanism underlying the host response to<br />
infection, bacterial adaptation, and pathogenesis. © <strong>2010</strong> by the Infectious Diseases Society <strong>of</strong> America. All rights reserved.<br />
No.434<br />
Author (s) : Janvilisri, T., Scaria, J., Gleed, R., Fubini, S., Bonkosky, M.M., Gröhn, Y.T., Chang, Y.-F.<br />
Title : Development <strong>of</strong> a microarray for identification <strong>of</strong> pathogenic Clostridium spp.<br />
Source : Diagnostic Microbiology and Infectious Disease, Volume 66, Issue 2, February <strong>2010</strong>, Pages 140-147<br />
Document Type : Article<br />
Keywords : Clostridium; Diagnosis; Microarray<br />
<strong>Abstract</strong> : In recent years, Clostridium spp. have rapidly reemerged as human and animal pathogens. The detection and identification <strong>of</strong><br />
pathogenic Clostridium spp. is therefore critical for clinical diagnosis and antimicrobial therapy. Traditional diagnostic<br />
techniques for clostridia are laborious, are time consuming, and may adversely affect the therapeutic outcome. In this study,<br />
we developed an oligonucleotide diagnostic microarray for pathogenic Clostridium spp. The microarray specificity was tested<br />
against 65 Clostridium isolates. The applicability <strong>of</strong> this microarray in a clinical setting was assessed with the use <strong>of</strong> mock<br />
stool samples. The microarray was successful in discriminating at least 4 species with the limit <strong>of</strong> detection as low as 104<br />
CFU/mL. In addition, the pattern <strong>of</strong> virulence and antibiotic resistance genes <strong>of</strong> tested strains were determined through the<br />
microarrays. This approach demonstrates the high-throughput detection and identification <strong>of</strong> Clostridium spp. and provides<br />
advantages over traditional methods. Microarray-based techniques are promising applications for clinical diagnosis and<br />
epidemiologic investigations. © <strong>2010</strong> Elsevier Inc. All rights reserved.<br />
No.435<br />
Author (s) : Jaovisidha, S., Suvikapakornkul, Y., Woratanarat, P., Subhadrabandhu, T., Nartthanarung, A., Siriwongpairat, P.<br />
Title : MR imaging <strong>of</strong> fat-containing tumours: The distinction between lipoma and liposarcoma<br />
Source : Singapore Medical Journal, Volume 51, Issue 5, May <strong>2010</strong>, Pages 418-423<br />
Document Type : Review<br />
Keywords : Lipoma; Liposarcoma; Magnetic resonance imaging; S<strong>of</strong>t tissue neoplasm<br />
<strong>Abstract</strong> : Introduction: This study aimed to retrospectively review the magnetic resonance (MR) imaging features <strong>of</strong> the lipomatous<br />
tumour in order to differentiate between lipoma and liposarcoma. Methods: The MR images <strong>of</strong> 38 patients (24 female and 14<br />
male with a mean age 48 years) in a consecutive five-year period, who had histologically verified lipoma (n is 29) and<br />
liposarcoma (n is 9), were retrospectively reviewed. The images were assessed for the number, site, size and margin <strong>of</strong> the<br />
lesions, as well as the signal intensity (homogenous, bright signal on T1-weighted [T1W] image, bright signal suppressed on<br />
T1W with fat-suppression image, bright signal on T2-weighted with fatsuppression image), the internal architecture (thin/thick<br />
septum, cystic change), the effect on the surrounding tissue (oedema, neurovascular involvement) and the enhancement<br />
pattern. Results: A partially ill-defined margin, neurovascular involvement, enhancing thick/nodular septum and a partially<br />
bright signal intensity on T1W images were statistically significant MR imaging features that favoured a diagnosis <strong>of</strong> liposarcoma<br />
(p-value is less than 0.0001). Male gender, an internal cystic change and surrounding s<strong>of</strong>t tissue oedema increased the risk <strong>of</strong><br />
liposarcoma approximately 2.8, 3.5 and 3.5 times, respectively, compared with the reference group (lipoma), but this was not<br />
a statistically significant finding. Thick/nodular septum was signif icantly associated with liposarcoma compared with lipoma<br />
(odds ratio 69.3, 95 percent confidence interval 5.2-3184.8, p-value is less than 0.0001). Conclusion: Statistically significant MR<br />
imaging features that favour a diagnosis <strong>of</strong> liposarcoma included a par tially ill-defined margin, neurovascular involvement,<br />
enhancing thick/ nodular septum, and a partially bright signal intensity on T1W images. The most statistically significant<br />
predictor <strong>of</strong> liposarcoma was thick/ nodular septum.<br />
160
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.436<br />
Author (s) : Jariyaboon, M., Davenport, A.J., Ambat, R., Connolly, B.J., Williams, S.W., Price, D.A.<br />
Title : Effect <strong>of</strong> cryogenic cooling on corrosion <strong>of</strong> friction stir welded AA7010-T7651<br />
Source : Anti-Corrosion Methods and Materials, Volume 57, Issue 2, <strong>2010</strong>, Pages 83-89<br />
Document Type : Article<br />
Keywords : Alloys; Cooling equipment; Corrosion; Cryogenic equipment; Friction welding; Joining processes<br />
<strong>Abstract</strong> : Purpose: The purpose <strong>of</strong> this paper is to study how cryogenic CO2 cooling during the welding process affects corrosion<br />
behaviour <strong>of</strong> friction stir welding (FSW) AA7010-T7651. Design/methodology/approach: Friction stir welded AA7010-T7651 was<br />
produced with a rotation speed <strong>of</strong> 288 rpm and a travel speed <strong>of</strong> 58 mm/min. The liquid CO2 was sprayed onto the weld<br />
centre line immediately after the toolpiece. The microstructures <strong>of</strong> welds in different regions were observed using Field<br />
Emission Gun Scanning Electron Microscope (FEG-SEM). The effect on the corrosion susceptibility was investigated using a gel<br />
visualisation test and potentiodynamic polarisation measurements using a micro-electrochemical technique. Findings: The<br />
main corrosion region for both FSWs AA7010-T7651 produced with and without cryogenic CO2 cooling is in the HAZ region,<br />
which exhibited intergranular attack. Cryogenic cooling does not show any influence on anodic reactivity <strong>of</strong> the weld region<br />
(both nugget and HAZ) compared to uncooled weld metal. However, the width <strong>of</strong> the reactive HAZ is reduced after cooling,<br />
as compared to the uncooled weld. The cooled welds show higher cathodic reactivity in the nugget region than does the<br />
nugget region <strong>of</strong> uncooled welds. Originality/value: There has been no previous work to investigate the effect <strong>of</strong> cryogenic<br />
CO2 cooling on the corrosion behaviour <strong>of</strong> FSW AA7010-T7651. The paper relates the microstructures <strong>of</strong> both uncooled and<br />
cooled welds to their anodic and cathodic reactivities using a micro-electrochemical technique. © Emerald Group Publishing<br />
Limited.<br />
No.437<br />
Author (s) : Jariyaboon, M.; Moller, P.; Dunin-Borkowski, R. E.; In, S. -I.; Chorkendorff, I.; Ambat, R.<br />
Title : The effect <strong>of</strong> atmospheric corona treatment on AA1050 aluminium<br />
Source : CORROSION SCIENCE Volume: 52 Issue: 6 Pages: 2155-2163 DOI: 10.1016/j.corsci.<strong>2010</strong>.01.035 Published: JUN <strong>2010</strong><br />
Document Type : Article<br />
Keywords : Aluminium; Polarization; Oxide coatings<br />
<strong>Abstract</strong> : The effect <strong>of</strong> atmospheric corona discharge on AM 050 aluminium surface was investigated using electrochemical polarization,<br />
SEM-EDX, FIB-SEM. and XPS. The corona treatment was performed with varying time (1, 5, and 15 min) in atmospheric air. A<br />
200 nm oxide layer was generated on AA1050 after the 15 min air corona treatment. A significant reduction in anodic and<br />
cathodic reactivities was observed starting from 1 min exposure, which further decreased with prolonged exposure (15 min)<br />
and after delayed testing (after 30 days). The reduction in surface reactivity is due to the formation <strong>of</strong> thicker and denser<br />
oxide film. (C) <strong>2010</strong> Elsevier Ltd. All rights reserved.<br />
No.438<br />
Author (s) : Jariyapan, N., Baimai, V., Poovorawan, Y., Roytrakul, S., Saeung, A., Thongsahuan, S., Suwannamit, S., Otsuka, Y., Choochote, W.<br />
Title : Analysis <strong>of</strong> female salivary gland proteins <strong>of</strong> the Anopheles barbirostris complex (Diptera: Culicidae) in Thailand<br />
Source : Parasitology Research, Volume 107, Issue 3, August <strong>2010</strong>, Pages 509-516<br />
Document Type : Article<br />
Keywords : saliva protein; Anopheles, anopheles barbirostris barbirostris, article, cytogenetics, liquid chromatography, mass<br />
spectrometry, nonhuman, nucleotide sequence, polyacrylamide gel electrophoresis, priority journal, protein analysis, protein<br />
polymorphism, salivary gland, species composition, Thailand; Anopheles barbirostris, Anopheles freeborni, Anopheles<br />
gambiae, Culicidae, Diptera<br />
<strong>Abstract</strong> : Electrophoretic protein pr<strong>of</strong>iles <strong>of</strong> female salivary glands <strong>of</strong> five sibling species within the Anopheles barbirostris complex,<br />
namely A. barbirostris species A1 (Forms A, B, and D), A2, A3, and A4 and Anopheles campestris-like (Forms B and E), were<br />
analyzed. At least eight major and several minor protein bands were detected in the glands <strong>of</strong> each species, <strong>of</strong> which each<br />
morphological region contained different major proteins. The protein pr<strong>of</strong>iles distinguished the five sibling species. The<br />
variability in major proteins among species was observed in the 40-48, 32-37, and 10-18 kDa ranges. No difference in protein<br />
pr<strong>of</strong>iles was found in different cytogenetic forms. Polymorphism <strong>of</strong> the protein pr<strong>of</strong>iles within species was only noted in<br />
species A4. The lowest major protein (marker) band <strong>of</strong> each species showed remarkably different relative mobility on SDSpolyacrylamide<br />
gels. NanoLC-MS analysis revealed that the marker protein <strong>of</strong> some species matched with a protein involving<br />
in blood feeding, gSG6, <strong>of</strong> Anopheles gambiae and Anopheles freeborni. These results might be useful for construction <strong>of</strong> an<br />
161
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
additional tool to distinguish the five sibling species and lead to further study on the evolution <strong>of</strong> blood feeding and<br />
pathogen transmission. © <strong>2010</strong> Springer-Verlag.<br />
No.439<br />
Author (s) : Jariyasethpong, T., Tribuddharat, C., Dejsirilert, S., Kerdsin, A., Tishyadhigama, P., Rahule, S., Sawanpanyalert, P., Yosapol, P.,<br />
Aswapokee, N.<br />
Title : MRSA carriage in a tertiary governmental hospital in Thailand: Emphasis on prevalence and molecular epidemiology<br />
Source : European Journal <strong>of</strong> Clinical Microbiology and Infectious Diseases, Volume 29, Issue 8, August <strong>2010</strong>, Pages 977-985<br />
Document Type : Article<br />
Keywords : aminoglycoside antibiotic agent, carbapenem, cephalosporin, penicillin G, quinoline derived antiinfective agent;<br />
adult, aged, article, bacterial gene, bacterium isolate, case control study, controlled study, drug use, female, gender, genetic<br />
polymorphism, hospital admission, hospital infection, human, infection risk, major clinical study, male, methicillin resistant<br />
Staphylococcus aureus, methicillin resistant Staphylococcus aureus infection, molecular<br />
epidemiology, nonhuman, prevalence, priority journal, risk factor, tertiary health care, Thailand; Adult, Aged, Aged, 80 and<br />
over, Bacterial Proteins, Bacteriophage Typing, Carrier State, Case-Control Studies, Cluster Analysis, Cross Infection, DNA<br />
Fingerprinting, Electrophoresis, Gel, Pulsed-Field, Female, Genotype, Hospitals, Humans, Male, Methicillin-Resistant<br />
Staphylococcus aureus, Middle Aged, Molecular Epidemiology, Polymerase Chain Reaction, Prevalence, Risk<br />
Factors, Sequence Analysis, DNA, Staphylococcal Infections, Thailand<br />
<strong>Abstract</strong> : We investigated prevalence and risk factors for methicillin-resistant Staphylococcus aureus (MRSA) in a case-control study<br />
performed in a 900-bed tertiary governmental healthcare facility in Bangkok, Thailand. Multivariate unconditional logistic<br />
regression was used to identify risk pr<strong>of</strong>iles for MRSA carriage. Phage typing, pulsed-field gel electrophoresis (PFGE),<br />
polymorphisms <strong>of</strong> the coa and spa genes, hypervariable region (HVR) <strong>of</strong> SCCmec, multi-locus sequence typing (MLST), and<br />
identification <strong>of</strong> ST30/ST8 mosaic chromosome by heteroduplex-polymerase chain reaction (heteroduplex-PCR) were used to<br />
demonstrate a clonal relationship. Fifty-seven <strong>of</strong> 619 in-patients (9.2%) were positive for MRSA. Risk factors were being male,<br />
long admission, low modified McCabe score, history <strong>of</strong> MRSA infection, and use <strong>of</strong> broad spectrum cephalosporin. Molecular<br />
typing results indicated close relatedness among MRSA isolates. Successful epidemic subtypes were recovered from many<br />
different wards. However, all subtypes with different multi-locus sequence types were single locus variants (SLVs) <strong>of</strong> ST239.<br />
Heteroduplex-PCR gave two positive bands from ST8/ST30 mosaic chromosomal structures in all SLVs indicating all isolates<br />
were <strong>of</strong> the ST239 origin. The burden <strong>of</strong> MRSA nosocomial infections is high in the governmental tertiary hospital. The sole<br />
ST239 and its SLVs identified in this hospital is striking and calls for better policy for infection control and prevention. © <strong>2010</strong><br />
Springer-Verlag.<br />
No.440<br />
Author (s) : Jaturapatporn, D., Moran, E., Obwanga, C., Husain, A.<br />
Title : Patients' experience <strong>of</strong> oxygen therapy and dyspnea: A qualitative study in home palliative care<br />
Source : Supportive Care in Cancer, Volume 18, Issue 6, June <strong>2010</strong>, Pages 765-770<br />
Document Type : Article<br />
Keywords : Dyspnea; Oxygen therapy; Qualitative study; Shortness <strong>of</strong> breath<br />
<strong>Abstract</strong> : Background Dyspnea is a common and distressing symptom in advanced cancer patients. Our preliminary work shows that in<br />
the home palliative care population sampled for this study, the prevalence <strong>of</strong> dyspnea is 29.5% and <strong>of</strong> those, 26.2% used<br />
oxygen therapy. Previous studies suggested that oxygen therapy can be a burden to patients. Purpose This study seeks to<br />
report the prevalence and describe the experience <strong>of</strong> dyspnea, pattern <strong>of</strong> oxygen use, and patients' perceived benefits<br />
and/or burdens <strong>of</strong> oxygen therapy in home palliative care patients receiving oxygen therapy. Methods Qualitative in-depth<br />
interviews, using an interview guide, were conducted with eight participants in their homes. Thematic analysis was performed<br />
using a framework approach. Results All patients in this project used oxygen most <strong>of</strong> the time. The descriptions <strong>of</strong> shortness<br />
<strong>of</strong> breath varied and were nonspecific. The patients identified more advantages than disadvantages. The advantages <strong>of</strong><br />
oxygen use included increased functional capacity, patients' perceiving oxygen as a life-saving intervention, as well as a<br />
symptom- management tool. The identified disadvantages were decreased mobility, discomfort related to the nasal prongs,<br />
barriers to accessing oxygen therapy and noise related to the equipment. Conclusion The advantages <strong>of</strong> oxygen usage<br />
outweighed the disadvantages for this sample <strong>of</strong> patients in the home palliative setting. © <strong>2010</strong> Springer-Verlag.<br />
162
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.441<br />
Author (s) : Jayathunge, M.P.H., Bowanwatanuwong, C., Maek-A-nantawat, W., Phonrat, B., Pitisuttithum, P.<br />
Title : Psychosocial burden <strong>of</strong> abnormal Pap smears among HIV-infected women at Chon Buri Hospital, Thailand<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 1, January <strong>2010</strong>, Pages 224-234<br />
Document Type : Article<br />
Keywords : Cervical cancer; HIV-infected; HPV; Human papillomavirus; Pap smears; Psychosocial burden; Thailand<br />
<strong>Abstract</strong> : This retrospective case-control study assessed the psychological burden <strong>of</strong> abnormal Pap smears, and their prevalence and<br />
characteristics among HIV-infected women attending an HIV clinic. Women with positive (n = 73) and negative Pap-smear<br />
results (n = 317) were assessed for psychosocial burden using 4 questionnaires: Psycho-Social Impact <strong>of</strong> Abnormal Pap<br />
Smears (PEAPS-Q), Hospital Anxiety and Depression Scale (HADS), Work Productivity and Impairment (WPAI) and the EURO-Qol<br />
Thermometer. The prevalence <strong>of</strong> pre-cervical cancer lesions in HIV infected woman was 17.5% (ASCUS 2.9%, LSIL 3.8%, HSIL<br />
7.4%, SCC 1.7%, and atypical glandular cells including adenocarcinoma 1.7%). HIV infected women with abnormal Pap smears<br />
showed higher anxiety levels on the HADS questionnaire (p = 0.015); this had a significant effect on regular daily activities (p =<br />
0.009) per the WPAI questionnaire compared to HIV positive women with normal Pap smear. Ever married HIV infected<br />
woman with an abnormal Pap smear had a significantly lower psychosocial burden using the PEAPS-Q questionnaire<br />
(p
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : Background: Familial hypercholesterolemia (FH) is an autosomal dominant disorder caused by mutations in the low density<br />
lipoprotein receptor (LDLR) gene. Two novel LDLR mutations, D151Y and M391T, had been previously identified in unrelated<br />
Thai patients with heterozygous FH. To confirm that these mutations cause FH, the functional characteristics <strong>of</strong> D151Y and<br />
M391T, which are located in the fourth cysteine repeat <strong>of</strong> the ligand-binding domain and in the sixth YWTD repeat <strong>of</strong> the<br />
epidermal growth factor precursor homology domain, respectively, were studied. Methods: CHO- ldlA7 cells were transfected<br />
with wild type and mutant LDLR cDNAs. Thereafter, the localization, expression, and ability <strong>of</strong> LDL uptake <strong>of</strong> LDLR were<br />
evaluated by confocal laser scanning microscope (CLSM), and flow cytometry. Results: CLSM revealed both D151Y and M391T<br />
LDLR were partially retained in the endoplasmic reticulum, with the remaining residual activity observed by LDL uptake.<br />
Similarly, flow cytometric analysis showed a significant reduction <strong>of</strong> LDLR expression to 18% and 38% and <strong>of</strong> LDL uptake to<br />
15% and 71% in D151Y and M391T LDLR, respectively. Conclusions: The transport defect <strong>of</strong> LDLR contributes to the<br />
pathology <strong>of</strong> FH. These data are useful for an insight inspires the development <strong>of</strong> novel lipid-lowering drugs with beneficial<br />
therapeutic value. © <strong>2010</strong> Elsevier B.V.<br />
No.444<br />
Author (s) : Jiang, J.<br />
Title : A theory <strong>of</strong> banking regulation<br />
Source : European Journal <strong>of</strong> Economics, Finance and Administrative Sciences, Issue 27, December <strong>2010</strong>, Pages 18-28<br />
Document Type : Article<br />
Keywords : Deposits insurance and capital adequacy; Reserve requirements<br />
<strong>Abstract</strong> : Aiming for better understanding and policy recommendations for the banking industry, this study seeks to explore optimal<br />
banking regulations with due considerations given to the CRA (Community Reinvestment Act) and FDIC (Federal Deposit<br />
Insurance Corporate) as representatively the most important regulatory tools in seeking efficiency in banking operations and<br />
preventions <strong>of</strong> system risk. In the procedure <strong>of</strong> theoretical pro<strong>of</strong>, this study not only incorporates problems <strong>of</strong> agency <strong>of</strong><br />
banks but also real sector and depositors' interactions in the seeking for private and public pr<strong>of</strong>it shares into the system.<br />
Therefore, the results <strong>of</strong> this study would provide venues <strong>of</strong> optimizing banking regulation policies that are explicitly<br />
expressed in the realization <strong>of</strong> series economic factors. It suggests the necessaries to form the strategic policies to monitor<br />
the movements <strong>of</strong> market risk premium with the proper estimation <strong>of</strong> future economic states, average, recession, or<br />
economic boom. The failure <strong>of</strong> monitoring these two components may theoretically imply the inefficient achievements <strong>of</strong><br />
subsidies and deposit insurances. © EuroJournals, Inc. <strong>2010</strong>.<br />
No.445<br />
Author (s) : Jiaranaikulwanitch, J., Boonyarat, C., Fokin, V.V., Vajragupta, O.<br />
Title : Triazolyl tryptoline derivatives as β-secretase inhibitors<br />
Source : Bioorganic and Medicinal Chemistry Letters, Volume 20, Issue 22, 15 November <strong>2010</strong>, Pages 6572-6576<br />
Document Type : Article<br />
Keywords : BACE1; BACE1 inhibitor; Binding mode; Cathepsin-D; Docking; Enzyme assay; JJCA-140; Tryptoline<br />
<strong>Abstract</strong> : Tryptoline, a core structure <strong>of</strong> ochrolifuanine E, which is a hit compound from virtual screening <strong>of</strong> the Thai herbal database<br />
against BACE1 was used as a scaffold for the design <strong>of</strong> BACE1 inhibitors. The tryptoline was linked with different side chains by<br />
1,2,3-triazole ring readily synthesized by catalytic azide-alkyne cycloaddition reactions. Twenty two triazolyl tryptoline<br />
derivatives were synthesized and screened for the inhibitory action against BACE1. JJCA-140 was the most potent inhibitor<br />
(IC50 = 1.49 μM) and was 100 times more selective for BACE1 than for Cat-D. © <strong>2010</strong> Elsevier Ltd. All rights reserved.<br />
No.446<br />
Author (s) : Jiarpinitnun, C., Kiessling, L.L.<br />
Title : Unexpected enhancement in biological activity <strong>of</strong> a GPCR ligand induced by an oligoethylene glycol substituent<br />
Source : Journal <strong>of</strong> the American Chemical Society, Volume 132, Issue 26, 7 July <strong>2010</strong>, Pages 8844-8845<br />
Document Type : Article<br />
Keywords : Biological activities, Biologically active molecules, Chemoattractants, Formyl peptide receptor, G protein coupled<br />
receptors, Half lives, N-formyl-methionine-leucine-phenylalanine, Oligoethylene glycols, Transmembranes; Amino<br />
acids, Biochemistry, Peptides, Polyethylene oxides, Signal transduction; Polyethylene glycols; ethylene glycol<br />
derivative, formylpeptide receptor, G protein coupled receptor; article, biological activity, cell<br />
migration, chemotaxis, human, human cell, intracellular signaling, structure activity relation; Cell<br />
164
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Membrane, Chemotaxis, Humans, Ligands, Peptides, Polyethylene Glycols, Receptors, Formyl Peptide, Structure-Activity<br />
Relationship, U937 Cells<br />
<strong>Abstract</strong> : Polyethylene glycol (PEG) is widely used, and many biologically active molecules are modified with oligoethylene glycol<br />
substituents to enhance their half-lives in circulation. The pervasive use <strong>of</strong> PEG substituents is partly due to their presumed<br />
inertness. Our investigation <strong>of</strong> formyl peptide receptor (FPR)-mediated chemotaxis reveals that oligoethylene glycol<br />
substitution can enhance the ability <strong>of</strong> the peptide chemoattractant N-formyl-methionine-leucine- phenylalanine (fMLF) to<br />
activate signal transduction through FPR, a transmembrane G-protein-coupled receptor. © <strong>2010</strong> American Chemical Society.<br />
No.447<br />
Author (s) : Jinapang, P., Prakob, P., Wongwattananard, P., Islam, N.E., Kirawanich, P.<br />
Title : Growth characteristics <strong>of</strong> mung beans and water convolvuluses exposed to 425-MHz electromagnetic fields<br />
Source : Bioelectromagnetics, Volume 31, Issue 7, October <strong>2010</strong>, Pages 519-527<br />
Document Type : Article<br />
Keywords : Exposure chamber; High-frequency continuous electromagnetic field; Seed germination; Seedling growth<br />
<strong>Abstract</strong> : Effects <strong>of</strong> high-frequency, continuous wave (CW) electromagnetic fields on mung beans (Vigna radiata L.) and water<br />
convovuluses (Ipomoea aquatica Forssk.) were studied at different growth stages (pre-sown seed and early seedling).<br />
Specifically, the effects <strong>of</strong> the electromagnetic source's power and duration (defined as power-duration level) on the growth<br />
<strong>of</strong> the two species were studied. Mung beans and water convolvuluses were exposed to electromagnetic fields inside a<br />
specially designed chamber for optimum field absorption, and the responses <strong>of</strong> the seeds to a constant frequency at various<br />
power levels and durations <strong>of</strong> exposure were monitored. The frequency used in the experiments was 425 MHz, the field<br />
strengths were 1mW, 100mW, and 10W, and the exposure durations were 1, 2, and 4 h. Results showthat germination<br />
enhancement is optimum for the mung beans at 100 mW/1 h power-duration level, while for water convolvuluses the<br />
optimum germination power-duration level was 1 mW/2 h. When both seed types were exposed at the early sprouting phase<br />
with their respective optimum power-duration levels for optimum seed growth, water convolvuluses showed growth<br />
enhancement while mung bean sprouts showed no effects.Water content analysis <strong>of</strong> the seeds suggests thermal effects only<br />
at higher field strength. © <strong>2010</strong> Wiley-Liss, Inc.<br />
No.448<br />
Author (s) : Jinawath, N., Morsberger, L., Norris-Kirby, A., Williams, L.M., Yonescu, R., Argani, P., Griffin, C.A., Murphy, K.M.<br />
Title : Complex rearrangement <strong>of</strong> chromosomes 1, 7, 21, 22 in Ewing sarcoma<br />
Source : Cancer Genetics and Cytogenetics, Volume 201, Issue 1, August <strong>2010</strong>, Pages 42-47<br />
Document Type : Article<br />
Keywords : cyclophosphamide, doxorubicin, etoposide, ifosfamide, RNA binding protein EWS, vincristine; article, cancer surgery, case<br />
report, child, chromosome 1, chromosome 21, chromosome 22, chromosome 7, chromosome banding pattern, chromosome<br />
breakage, chromosome rearrangement, chromosome translocation 1, chromosome translocation 21, chromosome<br />
translocation 7, controlled study, Ewing sarcoma, female, fluorescence in situ hybridization, gene fusion, human, human<br />
tissue, metaphase chromosome, multiple cycle treatment, preschool child, priority journal, reverse transcription polymerase<br />
chain reaction, RNA isolation, spectral karyotyping, thorax cancer; Base Sequence, Calmodulin-Binding Proteins, Child,<br />
Preschool, Chromosome Banding, Chromosomes, Human, DNA Primers, Female, Humans, In Situ Hybridization,<br />
Fluorescence, Reverse Transcriptase Polymerase Chain Reaction, RNA-Binding Proteins, Sarcoma, Ewing's, Spectral<br />
Karyotyping, Translocation, Genetic<br />
<strong>Abstract</strong> : The Ewing sarcoma (ES) family <strong>of</strong> tumors is characterized by nonrandom chromosomal translocations involving the EWSR1<br />
gene on chromosome 22 with one <strong>of</strong> the members <strong>of</strong> the ETS family <strong>of</strong> transcription factors. The majority <strong>of</strong> ES tumors are<br />
characterized by a balanced translocation t(11;22)(q24;q12), which results in the fusion <strong>of</strong> the 5' portion <strong>of</strong> EWSR1 gene with<br />
the 3'end <strong>of</strong> the FLI1 gene. Fusions with ERG, another member <strong>of</strong> the ETS family, occur in less than 10% <strong>of</strong> ES tumors, and<br />
can arise through complex chromosomal rearrangements. Here, we report a case <strong>of</strong> a 5-year-old female with an ES tumor in<br />
the thoracic region. G-banding and spectral karyotyping analysis demonstrated 46,XX,t(1;21;7)(q25;q22.3;q22). Metaphase<br />
fluorescence in situ hybridization (FISH) using the EWSR1 break-apart probe demonstrated a normal signal on both apparently<br />
normal chromosomes 22, and an additional EWSR1-5' signal on the derivative chromosome 21. Reverse-transcriptase<br />
polymerase chain reaction analysis <strong>of</strong> RNA isolated from the tumor demonstrated a EWSR1-ERG fusion transcript, fusing exon<br />
7 <strong>of</strong> EWSR1 and exon 11 <strong>of</strong> ERG. These results are consistent with an additional copy <strong>of</strong> the 5' portion <strong>of</strong> EWSR1, which<br />
inverted and then inserted on chromosome 21 and fused to the 3' end <strong>of</strong> ERG. To our knowledge, this is the first report <strong>of</strong> a<br />
165
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
EWSR1-ERG fusion in an ES tumor with an apparently duplicated 5' portion <strong>of</strong> EWSR1, and with a complex translocation<br />
involving chromosomes 1, 7, and 21. This case adds to the spectrum <strong>of</strong> genetic rearrangements identified in ES tumors. ©<br />
<strong>2010</strong> Elsevier Inc.<br />
No.449<br />
Author (s) : Jinawath, N., Vasoontara, C., Jinawath, A., Fang, X., Zhao, K., Yap, K.-L., Guo, T., Lee, C.S., Wang, W., Balgley, B.M., Davidson, B.,<br />
Wang, T.-L., Shih, I.-M.<br />
Title : Oncoproteomic analysis reveals co-upregulation <strong>of</strong> RELA and STAT5 in carboplatin resistant ovarian carcinoma<br />
Source : PLoS ONE, Volume 5, Issue 6, <strong>2010</strong>, Article number e11198<br />
Document Type : Article<br />
Keywords : 4 (2 aminoethylamino) 1,8 dimethylimidazo[1,2 a]quinoxaline, allograft inflammatory factor 1, apolipoprotein D, bms<br />
324825, carboplatin, cathepsin S, CD97 antigen, chymotrypsin A, dasatinib, folate binding protein, guanosine<br />
triphosphatase, interleukin 18, migration stimulating factor, pleckstrin, protein bcl xl, protein serine threonine kinase, protein<br />
tyrosine kinase, proteome, puromycin, short hairpin RNA, STAT5 protein, STAT5a protein, stromal interaction molecule<br />
1, syntenin, transcription factor RelA, ubiquitin protein ligase, unclassified drug, Wiskott Aldrich syndrome<br />
protein, antineoplastic agent, RELA protein, human, tumor protein; article, cancer patient, cancer resistance, capillary<br />
isoelectric focusing, chromatin immunoprecipitation, DNA binding, drug sensitization, gene control, gene<br />
silencing, human, nucleotide sequence, ovary carcinoma, primary tumor, promoter region, protein<br />
expression, proteomics, reverse transcription polymerase chain reaction, reversed phase liquid chromatography, tandem mass<br />
spectrometry, tumor recurrence, upregulation, apoptosis, drug resistance, electrospray mass spectrometry, enzyme linked<br />
immunosorbent assay, female, genetics, metabolism, ovary tumor, pathology, validation study; Antineoplastic<br />
Agents, Apoptosis, Carboplatin, Chromatin Immunoprecipitation, Drug Resistance, Neoplasm, Enzyme-Linked Immunosorbent<br />
Assay, Female, Humans, Neoplasm Proteins, Ovarian Neoplasms, Proteomics, Spectrometry, Mass, Electrospray<br />
Ionization, STAT5 Transcription Factor, Tandem Mass Spectrometry, Transcription Factor RelA, Up-Regulation<br />
<strong>Abstract</strong> : Background: Ovarian cancer is one <strong>of</strong> the most lethal types <strong>of</strong> female malignancy. Although most patients are initially<br />
responsive to platinum-based chemotherapy, almost all develop recurrent chemoresistant tumors and succumb to their<br />
diseases. Elucidating the pathogenesis underlying drug resistance is fundamental to the development <strong>of</strong> new therapeutics,<br />
leading to improved clinical outcomes in these patients. Methods and Findings: We compared the proteomes <strong>of</strong> paired<br />
primary and recurrent post-chemotherapy ovarian highgrade serous carcinomas from nine ovarian cancer patients using<br />
CIEF/Nano-RPLC coupled with ESI-Tandem MS. As compared to their primary tumors, more than half <strong>of</strong> the recurrent tumors<br />
expressed higher levels <strong>of</strong> several proteins including CP, FN1, SYK, CD97, AIF1, WNK1, SERPINA3, APOD, URP2, STAT5B and<br />
RELA (NF-kB p65), which were also validated by quantitative RT-PCR. Based on shRNA screening for the upregulated genes in<br />
in vitro carboplatin-resistant cells, we found that simultaneous knockdown <strong>of</strong> RELA and STAT5B was most effective in<br />
sensitizing tumor cells for carboplatin treatment. Similarly, the NF-kB inhibitor, BMS-345541, and the STAT5 inhibitor, Dasatinib,<br />
significantly enhanced cell sensitivity to carboplatin. Moreover, both RELA and STAT5 are known to bind to the promoter<br />
region <strong>of</strong> Bcl-X, regulating its promoter activity. In this regard, augmented Bcl-xL expression was detected in carboplatinresistant<br />
cells. Combined ectopic expression <strong>of</strong> RELA and STAT5B enhanced Bcl-xL promoter activity while treatment with<br />
BMS-345541 and Dasatinib decreased it. Chromatin immunoprecipitation <strong>of</strong> the Bcl-X promoter region using a STAT5 antibody<br />
showed induction <strong>of</strong> RELA and STAT5 DNA-binding segments both in nai{dotless}̈ve cells treated with a high concentration <strong>of</strong><br />
carboplatin as well as in carboplatin-resistant cells. Conclusions: Proteomic analysis identified RELA and STAT5 as two major<br />
proteins associated with carboplatin resistance in ovarian tumors. Our results further showed that NF-kB and STAT5 inhibitor<br />
could sensitize carboplatin-resistant cells and suggest that such inhibitors can be used to benefit patients with carboplatinresistant<br />
recurrent ovarian cancer. © <strong>2010</strong> Jinawath et al.<br />
No.450<br />
Author (s) : Jindadamrongwech, S., Tungbuppha, N., Chuncharunee, S., Butthep, P.<br />
Title : Hb tak and Hb Q-Thailand in thai patients are S-window hemoglobin variants revealed by high performance liquid<br />
chromatography<br />
Source : Hemoglobin, Volume 34, Issue 2, April <strong>2010</strong>, Pages 161-164<br />
Document Type : Article<br />
Keywords : Capillary electrophoresis (CE); HbQ-Thailand; HbTak; Hemoglobin variant; High performance liquid chromatography (HPLC)<br />
166
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : The S-window hemoglobin (Hb) variants revealed by high performance liquid chromatography (HPLC) were studied in 12 Thai<br />
individuals. The variants were identified, using DNA sequencing and multiplex amplification refractory mutation systempolymerase<br />
chain reaction (ARMS-PCR), to be six cases <strong>of</strong> Hb Tak [β147 (AC)], and six cases <strong>of</strong> Hb Q-Thailand<br />
[α74(EF3)Asp→His], respectively. By using the Capillarys 2-capillary zone electrophoresis (CE), Hb Tak and Hb Q-Thailand comigrated<br />
with Hb F in zone 7. This might pose a problem as the high Hb F conditions suggest a differential diagnosis. The Swindow<br />
Hb variants are mostly Hb Tak and Hb Q-Thailand in the Thai population rather than Hb S [β6(A3)Glu→Val]. The<br />
definite identification <strong>of</strong> Hb variants detected by HPLC or capillary electrophoresis (CE) requires DNA analysis. © Informa UK,<br />
Ltd.<br />
No.451<br />
Author (s) : Jintapattanakit, A., Peungvicha, P., Sailasuta, A., Kissel, T., Junyaprasert, V.B.<br />
Title : Nasal absorption and local tissue reaction <strong>of</strong> insulin nanocomplexes <strong>of</strong> trimethyl chitosan derivatives in rats<br />
Source : Journal <strong>of</strong> Pharmacy and Pharmacology, Volume 62, Issue 5, May <strong>2010</strong>, Pages 583-591<br />
Document Type : Article<br />
Keywords : Insulin nanocomplexes; Nasal delivery; Nasal histology; PEGylation; Trimethyl chitosan<br />
<strong>Abstract</strong> : Objectives The objective <strong>of</strong> this work was to explore the potential and safety <strong>of</strong> trimethyl chitosan (TMC) and PEGylated TMC<br />
for improved absorption <strong>of</strong> insulin after nasal administration. Methods The nasal absorption <strong>of</strong> insulin nanocomplexes <strong>of</strong> TMC<br />
or PEGylated TMC was evaluated in anaesthetized rats. Concomitantly, the histopathological effects <strong>of</strong> these nanocomplexes<br />
on rat nasal mucosa were studied using a perfusion fixation technique. Key findings All insulin nanocomplexes containing TMC<br />
or PEGylated TMC showed a 34-47% reduction in the blood glucose concentration, when the insulin absorption through the<br />
rat nasal mucosa was measured indirectly. In addition, the relative pharmacodynamic bioavailability (Fdyn) <strong>of</strong> the<br />
formulations was found to be dependent upon the charge ratio <strong>of</strong> insulin and polymer, regardless <strong>of</strong> polymer structure. The<br />
Fdyn apparently decreased with increasing charge ratio <strong>of</strong> insulin : polymer. Although acute alterations in nasal morphology<br />
by the formulations were affected by the charge ratio <strong>of</strong> insulin and polymer, the formulation <strong>of</strong> insulin/PEGylated TMC<br />
nanocomplexes was shown to be less toxic to the nasal epithelial membrane than insulin/TMC nanocomplexes. Conclusions<br />
PEGylated TMC nanocomplexes were a suitable absorption enhancer for nasal delivery <strong>of</strong> insulin. © <strong>2010</strong> Royal<br />
Pharmaceutical Society <strong>of</strong> Great Britain.<br />
No.452<br />
Author (s) : Jintaridth, P., Mutirangura, A.<br />
Title : Distinctive patterns <strong>of</strong> age-dependent hypomethylation in interspersed repetitive sequences<br />
Source : Physiological Genomics, Volume 41, Issue 2, April <strong>2010</strong>, Pages 194-200<br />
Document Type : Article<br />
Keywords : Aging; Genome-wide methylation; Global hypomethylation; Interspersed repetitive sequences<br />
<strong>Abstract</strong> : Interspersed repetitive sequences (IRSs) are a major contributor to genome size and may contribute to cellular functions. IRSs<br />
are subdivided according to size and functionally related structures into short interspersed elements, long interspersed<br />
elements (LINEs), DNA transposons, and LTR-retrotransposons. Many IRSs may produce RNA and regulate genes by a variety <strong>of</strong><br />
mechanisms. The majority <strong>of</strong> DNA methylation occurs in IRSs and is believed to suppress IRS activities. Global<br />
hypomethylation, or the loss <strong>of</strong> genome-wide methylation, is a common epigenetic event not only in senescent cells but<br />
also in cancer cells. Loss <strong>of</strong> LINE-1 methylation has been characterized in many cancers. Here, we evaluated the methylation<br />
levels <strong>of</strong> peripheral blood mononuclear cells <strong>of</strong> LINE-1, Alu, and human endogenous retrovirus K (HERV-K) in 177 samples<br />
obtained from volunteers between 20 and 88 yr <strong>of</strong> age. Age was negatively associated with methylation levels <strong>of</strong> Alu (r = -<br />
0.452, P < 10-3) and HERV-K (r = -0.326, P < 10-3) but not LINE-1 (r = 0.145, P = 0.055). Loss <strong>of</strong> methylation <strong>of</strong> Alu<br />
occurred during ages 34-68 yr, and loss <strong>of</strong> methylation <strong>of</strong> HERV-K occurred during ages 40-63 yr and again during ages 64-83<br />
yr. Interestingly, methylation <strong>of</strong> Alu and LINE-1 are directly associated, particularly at ages 49 yr and older (r = 0.49, P < 10-<br />
3). Therefore, only some types <strong>of</strong> IRSs lose methylation at certain ages. Moreover, Alu and HERV-K become hypomethylated<br />
differently. Finally, there may be several mechanisms <strong>of</strong> global methylation. However, not all <strong>of</strong> these mechanisms are agedependent.<br />
This finding may lead to a better understanding <strong>of</strong> not only the biological causes and consequences <strong>of</strong> genomewide<br />
hypomethylation but also the role <strong>of</strong> IRSs in the aging process. Copyright © <strong>2010</strong> the American Physiological Society.<br />
No.453<br />
Author (s) : Jirapaet, K., Jirapaet, V., Sritipsukho, S.<br />
167
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Safety <strong>of</strong> initiating early enteral feeding with slow volume advancement in preterm infants<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 10, October <strong>2010</strong>, Pages 1177-1187<br />
Document Type : Article<br />
Keywords : Early feeding; Human milk; Late-onset sepsis; Necrotizing enterocolitis; Preterm infant; Slow volume advancement<br />
<strong>Abstract</strong> : Objective: To determine the safety <strong>of</strong> enteral feeding within 24 hours <strong>of</strong> life with slow volume increase on the incidence <strong>of</strong><br />
necrotizing enterocolitis (NEC) and late-onset sepsis (LOS). Design: Prospective descriptive study. Material and Method:<br />
Between January 1998 and December 2001, 117 preterm infants with birth weight < 1,750 g were recruited prospectively, 102<br />
in the human-milk-fed group (HMG) and 15 in the formula-fed group (FG). Feeds were advanced by increments <strong>of</strong> 10 mL/kg/d,<br />
aimed at 150 mL/kg/d in 15 days. Charts <strong>of</strong> 146 preterm infants admitted during 1996-1997 were reviewed for pre-study<br />
incidences <strong>of</strong> NEC and LOS. Results: NEC developed 3.92% in HMG and 20% in FG (p =.044). LOS developed 2.94% in HMG<br />
and 13.33% in FG (p = 0.122). The overall incidence <strong>of</strong> NEC was almost similar (5.98% vs. 6.16%) while that <strong>of</strong> LOS was lower<br />
(4.27% vs. 12.32%) when compared to the pre-study incidences. Conclusion: The present study provides a practice that<br />
seems to reduce LOS risk without increasing NEC risk and confirms the protective effect <strong>of</strong> human milk against NEC.<br />
No.454<br />
Author (s) : Jirapongsananuruk, O., Pongpreuksa, S., Sangacharoenkit, P., Visitsunthorn, N., Vichyanond, P.<br />
Title : Identification <strong>of</strong> the etiologies <strong>of</strong> chronic urticaria in children: A prospective study <strong>of</strong> 94 patients<br />
Source : Pediatric Allergy and Immunology, Volume 21, Issue 3, May <strong>2010</strong>, Pages 508-514<br />
Document Type : Article<br />
Keywords : Autologous serum skin test; Chronic autoimmune urticaria; Chronic urticaria; Food allergy; Skin prick test<br />
<strong>Abstract</strong> : The etiologies <strong>of</strong> chronic urticaria (CU) in childhood remains incompletely understood because <strong>of</strong> limited data in children.<br />
The objective <strong>of</strong> this study was to examine some <strong>of</strong> the possible etiologies <strong>of</strong> CU in children by focusing on the functional<br />
autoantibody to FcRIα and IgE, thyroid autoimmunity, urticarial vasculitis, parasitic infestation and food allergy. Children 4-15<br />
yr <strong>of</strong> age with CU were investigated for complete blood count, erythrocyte sedimentation rate (ESR), antinuclear antibody<br />
(ANA), CH50, free-T4 (FT 4), thyroid stimulating hormone (TSH), anti-thyroglobulin and anti-microsomal antibody, autologous<br />
serum skin test (ASST), skin prick tests (SPT) for foods, food challenges, and stool examination for parasites. Ninety-four<br />
children who met the criteria for CU were recruited. Patients with physical urticaria were excluded. Eosinophilia and elevated<br />
ESR were found in 23% and 13%, respectively. High ANA titers were found in 2%. None <strong>of</strong> these patients had clinical features<br />
<strong>of</strong> urticarial vasculitis, abnormal CH 50 level, abnormal TSH and FT4. Anti-thyroglobulin and anti-microsomal antibodies were<br />
not detected. Positive ASST was found in 38%. There were no differences in medication requirement and CU remission<br />
between patients with positive and negative ASST. Parasites were found in 5% without clinical correlation. SPT to foods was<br />
positive in 35%. Positive food challenges were found in six/nine patients with positive history <strong>of</strong> food allergy and two/seven<br />
patients with negative history. Food avoidance was beneficial to the subgroup <strong>of</strong> patients with positive history <strong>of</strong> food allergy<br />
only. © 2009 John Wiley & Sons A/S.<br />
No.455<br />
Author (s) : Jirarattanasopa, N., Tantikul, C., Vichyanond, P., Pacharn, P., Visitsunthorn, N., Suttinont, P., Jirapongsananuruk, O.<br />
Title : A combination <strong>of</strong> intravenous immunoglobulin and pulse methylprednisolone extended survival in pulmonary alveolar<br />
proteinosis with chronic interstitial pneumonitis: A case report<br />
Source : Asian Pacific Journal <strong>of</strong> Allergy and Immunology, Volume 28, Issue 2-3, June <strong>2010</strong>, Pages 200-205<br />
Document Type : Article<br />
Keywords : Chronic interstitial pneumonitis; Intravenous immunoglobulin; Pulmonary alveolar proteinosis; Pulse methylprednisolone<br />
<strong>Abstract</strong> : Pulmonary alveolar proteinosis (PAP) is characterized by intra-alveolar accumulation <strong>of</strong> lipoproteinaceous material. The<br />
severe chronic pulmonary disease and susceptibility to pulmonary infection is a prominent feature <strong>of</strong> the disease. We<br />
reported a case <strong>of</strong> postnatal-onset PAP and chronic interstitial pneumonitis in a girl with chronic respiratory distress since she<br />
was 5 months <strong>of</strong> age. A lung biopsy confirmed the diagnosis. The therapeutic bronchoalveolar lavages, a short trial <strong>of</strong><br />
granulocyte colonystimulation factor (G-CSF) and a combination <strong>of</strong> low dose methylprednisolone and hydroxychloroquine<br />
were used at different times without noting satisfactory improvement. Intravenous immunoglobulin (IVIG) and pulse<br />
methylprednisolone were given monthly with gradual recovery. She did not require oxygen supplement after 21 months <strong>of</strong><br />
this combination. Our report suggested that IVIG and pulse methylprednisolone might have a potential role in the treatment<br />
<strong>of</strong> PAP with chronic interstitial pneumonitis.<br />
168
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.456<br />
Author (s) : Jirasiritham, S., Khunprakant, R., Techawathanawanna, N., Jirasiritham, S., Mavichak, V.<br />
Title : Treatment <strong>of</strong> simultaneous acute antibody-mediated rejection and acute cellular rejection with alemtuzumab in kidney<br />
transplantation: a case report.<br />
Source : Transplantation proceedings, Volume 42, Issue 3, April <strong>2010</strong>, Pages 987-989<br />
Document Type : Article<br />
Keywords : alemtuzumab, alloantibody, cancer antibody, creatinine, immunoglobulin, monoclonal antibody; adult, article, blood, case<br />
report, chronic kidney failure, female, glomerulonephritis, graft rejection, human, immunology, kidney transplantation, living<br />
donor, lymphocyte count, lymphocyte depletion, plasmapheresis, treatment outcome; Adult, Antibodies,<br />
Monoclonal, Antibodies, Neoplasm, Creatinine, Female, Glomerulonephritis, Graft Rejection, Humans, Immunoglobulins,<br />
Intravenous, Isoantibodies, Kidney Failure, Chronic, Kidney Transplantation, Living Donors, Lymphocyte Count, Lymphocyte<br />
Depletion, Plasmapheresis, Treatment Outcome<br />
<strong>Abstract</strong> : This is a case report <strong>of</strong> a living related donor kidney transplantation using basiliximab induction and maintenance<br />
immunosuppression with cyclosporine, mycophenolate sodium, and steroid. On the second posttransplant day, the patient<br />
developed acute antibody-mediated rejection, which was treated with plasmapheresis and intravenous immunoglobulin<br />
(IVIG). Five days later, the graft had still not responded to the treatment. Another biopsy revealed additional acute cellular<br />
rejection (Banff IIA). As alemtuzumab can rapidly deplete T and B lymphocytes, monocytes, and natural killer cells, the<br />
patient was treated with alemtuzumab (30 mg subcutaneously) together with methylprednisolone (500 mg) and two more<br />
plasmaphereses. The kidney graft responded within 48 hours, producing more than 4 L <strong>of</strong> urine per day. The total<br />
lymphocyte decreased from 530/microL to 50/microL remaining in the 50 to 220/microL range. The patient received<br />
valgancyclovir and cotrimoxazole as infection prophylaxis. The kidney graft responded well to the rescue treatment and the<br />
patient was discharged with a serum creatinine <strong>of</strong> 1.1 mg/mL and has been uneventfully followed in the outpatient clinic for<br />
8 months. Today, with the potent, effective, and selective immunosuppressive regimens, the rate and severity <strong>of</strong> acute<br />
cellular rejection in kidney transplantation has decreased in most centers. However, the rate <strong>of</strong> acute antibody-mediated<br />
rejection has increased to levels greater than those <strong>of</strong> acute cellular rejection in many centers. Acute antibody-mediated<br />
rejection is more difficult and expensive to treat successfully. The treatment <strong>of</strong> acute antibody-mediated rejection included<br />
plasmapheresis and IVIG. Herein we have reported a case <strong>of</strong> kidney transplantation simultaneously developing acute<br />
antibody-mediated and acute cellular rejection; the patient was successfully treated with alemtuzumab. Copyright (c) <strong>2010</strong><br />
Elsevier Inc. All rights reserved.<br />
No.457<br />
Author (s) : Jitnarin, N., Kosulwat, V., Rojroongwasinkul, N., Boonpraderm, A., Haddock, C.K., Poston, W.S.C.<br />
Title : Risk factors for overweight and obesity among Thai adults: Results <strong>of</strong> the national Thai food consumption survey<br />
Source : Nutrients, Volume 2, Issue 1, January <strong>2010</strong>, Pages 60-74<br />
Document Type : Article<br />
Keywords : Dietary intake; Overweight/obesity; SES; Smoking; Thailand<br />
<strong>Abstract</strong> : We evaluated the associations between overweight and obesity and socioeconomic status (SES), behavioral factors, and<br />
dietary intake in Thai adults. A nationally representative sample <strong>of</strong> 6,445 Thais adults (18-70 years) was surveyed during 2004-<br />
2005. Information including demographics, SES characteristics, dietary intake, and anthropometrics were obtained. Overall,<br />
35.0% <strong>of</strong> men, and 44.9% <strong>of</strong> women were overweight or obese (BMI ≥ 23 kg/m2) using the Asian cut-points. Regression<br />
models demonstrated that age was positively associated with being overweight in both genders. In gender-stratified analyses,<br />
male respondents who were older, lived in urban areas, had higher annual household income, and did not smoke were more<br />
likely to be classified as overweight and obese. Women who were older, had higher education, were not in a marriage-like<br />
relationship and were in semi-pr<strong>of</strong>essional occupation were at greater risk for being overweight and obese. High carbohydrate<br />
and protein intake were found to be positively associated with BMI whereas the frequent use <strong>of</strong> dairy foods was found to be<br />
negatively associated with BMI among men. The present study found that SES factors are associated with being classified as<br />
overweight and obese in Thai adults, but associations were different between genders. Health promotion strategies regarding<br />
obesity and its related co-morbidity are necessary. © <strong>2010</strong> by the authors; licensee Molecular Diversity Preservation<br />
<strong>International</strong>, Basel, Switzerland.<br />
No.458<br />
Author (s) : Jitprasutwit, S., Thaewpia, W., Muangsombut, V., Lulitanond, A., Leelayuwat, C., Lertmemongkolchai, G., Korbsrisate, S.<br />
169
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Effect <strong>of</strong> acidic pH on the invasion efficiency and the type III Secretion system <strong>of</strong> Burkholderia thailandensis<br />
Source : Journal <strong>of</strong> Microbiology, Volume 48, Issue 4, <strong>2010</strong>, Pages 526-532<br />
Document Type : Article<br />
Keywords : acidic pH; B. thailandensis; invasion; type III secretion system<br />
<strong>Abstract</strong> : Burkholderia thailandensis is a close relative <strong>of</strong> Burkholderia pseudomallei. These organisms are very similar, but B.<br />
thailandensis is far less virulent than B. pseudomallei. Nucleotide sequencing and analysis <strong>of</strong> 14 B. thailandensis isolates<br />
revealed variation in the regions coding for the type III secreted BipD protein. The degree <strong>of</strong> B. thailandensis BipD sequence<br />
variation was greater than that found in B. pseudomallei. Western blot analysis indicated that, unlike B. pseudomallei, B.<br />
thailandensis type III secreted proteins including BipD and BopE could not be detected in the supernatant <strong>of</strong> culture medium<br />
unless induced by acidic conditions. In addition, culturing B. thailandensis under acidic growth conditions (pH 4.5) can induce<br />
the ability <strong>of</strong> this bacterium to invade human respiratory epithelial cells A549. The identification <strong>of</strong> an environmental stimulus<br />
that increases the invasion capability <strong>of</strong> B. thailandensis invasion is <strong>of</strong> value for those who would like to use this bacterium as<br />
a model to study B. pseudomallei virulence. © <strong>2010</strong> The Microbiological Society <strong>of</strong> Korea and Springer-Verlag Berlin<br />
Heidelberg.<br />
No.459<br />
Author (s) : Jitramontree, N., Schoenfelder, D.P.<br />
Title : Exercise promotion: Walking in elders<br />
Source : Journal <strong>of</strong> Gerontological Nursing, Volume 36, Issue 11, November <strong>2010</strong>, Pages 10-18<br />
Document Type : Article<br />
Keywords : aged, article, exercise, geriatric assessment, health promotion, human, practice guideline, risk factor, treatment outcome;<br />
Aged, Exercise, Geriatric Assessment, Guidelines as Topic, Health Promotion, Humans, Outcome and Process Assessment<br />
(Health Care), Risk Factors<br />
<strong>Abstract</strong> : –<br />
No.460<br />
Author (s) : Jitrapakdee, S., Wutthisathapornchai, A., Wallace, J.C., MacDonald, M.J.<br />
Title : Regulation <strong>of</strong> insulin secretion: Role <strong>of</strong> mitochondrial signaling<br />
Source : Diabetologia, Volume 53, Issue 6, June <strong>2010</strong>, Pages 1019-1032<br />
Document Type : Review<br />
Keywords : Coupling factors; Glucose-stimulated insulin secretion; Insulin secretion; Mitochondrial metabolism; Pyruvate<br />
cycling; Review<br />
<strong>Abstract</strong> : Pancreatic beta cells are specialised endocrine cells that continuously sense the levels <strong>of</strong> blood sugar and other fuels and, in<br />
response, secrete insulin to maintain normal fuel homeostasis. During postprandial periods an elevated level <strong>of</strong> plasma<br />
glucose rapidly stimulates insulin secretion to decrease hepatic glucose output and promote glucose uptake into other<br />
tissues, principally muscle and adipose tissues. Beta cell mitochondria play a key role in this process, not only by providing<br />
energy in the form <strong>of</strong> ATP to support insulin secretion, but also by synthesising metabolites (anaplerosis) that can act, both<br />
intra- and extramitochondrially, as factors that couple glucose sensing to insulin granule exocytosis. ATP on its own, and<br />
possibly modulated by these coupling factors, triggers closure <strong>of</strong> the ATP-sensitive potassium channel, resulting in membrane<br />
depolarisation that increases intracellular calcium to cause insulin secretion. The metabolic imbalance caused by chronic<br />
hyperglycaemia and hyperlipidaemia severely affects mitochondrial metabolism, leading to the development <strong>of</strong> impaired<br />
glucose-induced insulin secretion in type 2 diabetes. It appears that the anaplerotic enzyme pyruvate carboxylase participates<br />
directly or indirectly in several metabolic pathways which are important for glucose-induced insulin secretion, including: the<br />
pyruvate/malate cycle, the pyruvate/citrate cycle, the pyruvate/isocitrate cycle and glutamate-dehydrogenase-catalysed αketoglutarate<br />
production. These four pathways enable 'shuttling' or 'recycling' <strong>of</strong> these intermediate(s) into and out <strong>of</strong><br />
mitochondrion, allowing continuous production <strong>of</strong> intracellular messenger(s). The purpose <strong>of</strong> this review is to present an<br />
account <strong>of</strong> recent progress in this area <strong>of</strong> central importance in the realm <strong>of</strong> diabetes and obesity research. © <strong>2010</strong> Springer-<br />
Verlag.<br />
No.461<br />
Author (s) : Jittapalapong, S., Inpankaew, T., Pinyopanuwat, N., Chimnoi, W., Kengradomkij, C., Wongnarkpet, S., Maruyama, S., Lekkla, A.,<br />
Sukthana, Y.<br />
170
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Epidemiology <strong>of</strong> toxoplasma gondii infection <strong>of</strong> stray cats in Bangkok, Thailand<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 1, January <strong>2010</strong>, Pages 13-18<br />
Document Type : Article<br />
Keywords : Sabin-Feldman dye test; Stray cats; Thailand; Toxoplasma gondii<br />
<strong>Abstract</strong> : The objective <strong>of</strong> this study was to investigate the epidemiology <strong>of</strong> toxoplasmosis in stray cats in Bangkok. Sera were collected<br />
during 2006 and examined by Sabin-Feldman dye test. Five hundred sixty-four male and 926 female cats in and around<br />
monasteries from 50 districts were collected. Toxoplasma gondii was detected in 72 (4.8%) <strong>of</strong> 1,490 cats. The prevalence was<br />
significantly higher in females (5.6%) than in males (3.6%). Cats more than 5 years old had the highest infection rate (5.1%).<br />
Fifty-six percent (28/50) <strong>of</strong> areas were positive for T. gondii in cats. Our results show T. gondii is widespread in stray cats in<br />
Bangkok. It is essential to control the number <strong>of</strong> stray cats in order to reduce the transmission <strong>of</strong> toxoplasmosis to animals<br />
and humans.<br />
No.462<br />
Author (s) : Jittawuttipoka, T., Sallabhan, R., Vattanaviboon, P., Fuangthong, M., Mongkolsuk, S.<br />
Title : Mutations <strong>of</strong> ferric uptake regulator (fur) impair iron homeostasis, growth, oxidative stress survival, and virulence <strong>of</strong><br />
Xanthomonas campestris pv. Campestris<br />
Source : Archives <strong>of</strong> Microbiology, Volume 192, Issue 5, May <strong>2010</strong>, Pages 331-339<br />
Document Type : Article<br />
Keywords : Ferric uptake regulator; Fur; Iron; Plant pathogen; Xanthomonas<br />
<strong>Abstract</strong> : Iron is essential in numerous cellular functions. Intracellular iron homeostasis must be maintained for cell survival and<br />
protection against iron's toxic effects. Here, we characterize the roles <strong>of</strong> Xanthomonas campestris pv. campestris (Xcc) fur,<br />
which encodes an iron sensor and a transcriptional regulator that acts in iron homeostasis, oxidative stress, and virulence.<br />
Herein, we isolated spontaneous Xcc fur mutants that had high intracellular iron concentrations due to constitutively high<br />
siderophore levels and increased expression <strong>of</strong> iron transport genes. These mutants also had reduced aerobic plating<br />
efficiency and resistance to peroxide killing. Moreover, one fur mutant was attenuated on a host plant, thus indicating that fur<br />
has important roles in the virulence <strong>of</strong> X. campestris pv. campestris. © <strong>2010</strong> Springer-Verlag.<br />
No.463<br />
Author (s) : Jittivadhna, K., Ruenwongsa, P., Panijpan, B.<br />
Title : Beyond textbook illustrations: Hand-held models <strong>of</strong> ordered DNA and protein structures as 3D supplements to enhance<br />
student learning <strong>of</strong> helical biopolymers<br />
Source : Biochemistry and Molecular Biology Education, Volume 38, Issue 6, November <strong>2010</strong>, Pages 359-364<br />
Document Type : Article<br />
Keywords : DNA; Helical structure; Polypeptide; Science education<br />
<strong>Abstract</strong> : Textbook illustrations <strong>of</strong> 3D biopolymers on printed paper, regardless <strong>of</strong> how detailed and colorful, suffer from its twodimensionality.<br />
For beginners, computer screen display <strong>of</strong> skeletal models <strong>of</strong> biopolymers and their animation usually does<br />
not provide the at-a-glance 3D perception and details, which can be done by good hand-held models. Here, we report a<br />
study on how our students learned more from using our ordered DNA and protein models assembled from colored<br />
computer-printouts on transparency film sheets that have useful structural details. Our models (reported in BAMBED 2009),<br />
having certain distinguished features, helped our students to grasp various aspects <strong>of</strong> these biopolymers that they usually find<br />
difficult. Quantitative and qualitative learning data from this study are reported. © <strong>2010</strong> <strong>International</strong> Union <strong>of</strong> Biochemistry<br />
and Molecular Biology, Inc.<br />
No.464<br />
Author (s) : Johnson, H., Meeyai, A., Coker, R.<br />
Title : Potential for greater coherence in EUROPEAN influenza surveillance<br />
Source : European Journal <strong>of</strong> Public Health, Volume 20, Issue 5, October <strong>2010</strong>, Pages 488-489<br />
Document Type : Note<br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
171
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.465<br />
Author (s) : Jonmalung, J., Prammananan, T., Leechawengwongs, M., Chaiprasert, A.<br />
Title : Surveillance <strong>of</strong> pyrazinamide susceptibility among multidrug-resistant Mycobacterium tuberculosis isolates from Siriraj<br />
Hospital, Thailand<br />
Source : BMC Microbiology, Volume 10, <strong>2010</strong>, Article number 223<br />
Document Type : Article<br />
Keywords : ethambutol, isoniazid, pyrazinamide, rifampicin, streptomycin, amidase, antiinfective agent, PncA protein, Mycobacterium<br />
tuberculosis, tuberculostatic agent; adolescent, adult, aged, antibiotic resistance, antibiotic sensitivity, article, bacterial<br />
growth, bacterial mutation, bacterial strain, child, controlled study, culture medium, disease surveillance, drug<br />
activity, female, human, male, multidrug resistance, multidrug resistant tuberculosis, Mycobacterium<br />
tuberculosis, nonhuman, nucleotide sequence, preschool child, school child, sensitivity and specificity, Thailand, drug<br />
effect, enzymology, genetics, health survey, hospital, isolation and purification, metabolism, microbiological<br />
examination, microbiology, middle aged, mutation, tuberculosis; Mycobacterium tuberculosis; Adolescent, Adult, Aged, Aged,<br />
80 and over, Amidohydrolases, Anti-Bacterial Agents, Antitubercular Agents, Child, Child, Preschool, Drug Resistance, Multiple,<br />
Bacterial, Female, Hospitals, Humans, Male, Microbial Sensitivity Tests, Middle Aged, Mutation, Mycobacterium<br />
tuberculosis, Population Surveillance, Pyrazinamide, Thailand, Tuberculosis, Young Adult<br />
<strong>Abstract</strong> : Background. Susceptibility testing <strong>of</strong> pyrazinamide (PZA) against Mycobacterium tuberculosis is difficult to perform because<br />
the acidity <strong>of</strong> culture medium that is required for drug activity also inhibits the growth <strong>of</strong> bacteria. In Thailand, very limited<br />
information has been generated on PZA resistance, particularly among multidrug-resistant tuberculosis (MDR-TB) isolated from<br />
Thailand. Only two studies on PZA susceptibility among Thai M. tuberculosis strains have been reported; one used a<br />
pyrazinamidase assay, and the other used the BACTEC 460 TB for PZA susceptibility testing. In this study, we determined the<br />
percentage <strong>of</strong> strains possessing pyrazinamide resistance among pan-susceptible M. tuberculosis and MDR-TB isolates by using<br />
the pyrazinamidase assay, BACTEC MGIT 960 PZA method and pncA sequencing, and assessed the correlation in the data<br />
generated using these methods. The type and frequency <strong>of</strong> mutations in pncA were also determined. Results. Overall, 150 M.<br />
tuberculosis isolates, consisting <strong>of</strong> 50 susceptible and 100 MDR-TB isolates, were tested for PZA susceptibility by BACTEC MGIT<br />
960 PZA, the pyrazinamidase assay and pncA sequencing. The study indicated PZA resistance in 6% and 49% <strong>of</strong> susceptible<br />
and MDR-TB isolates, respectively. In comparison to the BACTEC MGIT 960 PZA, the PZase assay showed 65.4% sensitivity and<br />
100% specificity, whereas pncA sequencing showed 75% sensitivity and 89.8% specificity. Twenty-four mutation types were<br />
found in this study, with the most frequent mutation (16%) being His71Asp. Of these mutations, eight have not been<br />
previously described. The Ile31Ser and Ile31Thr mutations were found both in PZA susceptible and resistant isolates,<br />
suggesting that mutation <strong>of</strong> this codon might not play a role on PZA resistance. Conclusions. Our findings suggest that<br />
phenotypic susceptibility testing is still essential for the detection <strong>of</strong> PZA resistance, especially for MDR-TB isolates. Some<br />
mutations were not associated with resistance and could lead to misinterpretation <strong>of</strong> the genotypic methods. This<br />
information could be helpful for clinicians in managing tuberculosis patients and frequencies, and the types <strong>of</strong> pncA<br />
mutations should <strong>of</strong>fer baseline information on PZA resistance. © <strong>2010</strong> Jonmalung et al; licensee BioMed Central Ltd.<br />
No.466<br />
Author (s) : Junjhon, J., Edwards, T.J., Utaipat, U., Bowman, V.D., Holdaway, H.A., Zhang, W., Keelapang, P., Puttikhunt, C., Perera, R.,<br />
Chipman, P.R., Kasinrerk, W., Malasit, P., Kuhn, R.J., Sittisombut, N.<br />
Title : Influence <strong>of</strong> pr-M cleavage on the heterogeneity <strong>of</strong> extracellular dengue virus particles<br />
Source : Journal <strong>of</strong> Virology, Volume 84, Issue 16, August <strong>2010</strong>, Pages 8253-8358<br />
Document Type : Article<br />
Keywords : antibody, prM protein, Flavivirus, virus envelope protein; amino acid sequence, article, controlled study, cryoelectron<br />
microscopy, Dengue virus 2, immunoprecipitation, nonhuman, priority journal, serotype, virus mutant, virus particle, Dengue<br />
virus, isolation and purification, metabolism, physiology, ultrastructure, virion, virus assembly; Dengue virus; Cryoelectron<br />
Microscopy, Dengue Virus, Immunoprecipitation, Viral Envelope Proteins, Virion, Virus Assembly<br />
<strong>Abstract</strong> : During dengue virus replication, an incomplete cleavage <strong>of</strong> the envelope glycoprotein prM, generates a mixture <strong>of</strong> mature<br />
(prM-less) and prM-containing, immature extracellular particles. In this study, sequential immunoprecipitation and<br />
cryoelectron microscopy revealed a third type <strong>of</strong> extracellular particles, the partially mature particles, as the major prMcontaining<br />
particles in a dengue serotype 2 virus. Changes in the proportion <strong>of</strong> viral particles in the pr-M junction mutants<br />
exhibiting altered levels <strong>of</strong> prM cleavage suggest that the partially mature particles may represent an intermediate<br />
172
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
subpopulation in the virus maturation pathway. These findings are consistent with a model suggesting the progressive mode<br />
<strong>of</strong> prM cleavage. Copyright © <strong>2010</strong>, American Society for Microbiology. All Rights Reserved.<br />
No.467<br />
Author (s) : Jutapakdeegul, N., Afadlal, S., Polaboon, N., Phansuwan-Pujito, P., Govitrapong, P.<br />
Title : Repeated restraint stress and corticosterone injections during late pregnancy alter GAP-43 expression in the hippocampus and<br />
prefrontal cortex <strong>of</strong> rat pups<br />
Source : <strong>International</strong> Journal <strong>of</strong> Developmental Neuroscience, Volume 28, Issue 1, February <strong>2010</strong>, Pages 83-90<br />
Document Type : Article<br />
Keywords : Corticosterone; GAP-43; Hippocampus; Prefrontal coretx; Prenatal stress<br />
<strong>Abstract</strong> : In the <strong>of</strong>fspring <strong>of</strong> prenatal stress animals, overactivity and impaired negative feedback regulation <strong>of</strong> the hypothalamicpituitary-adrenal<br />
axis are consistent finding. However, little was known about how prenatal stress can permanently alter<br />
developmental trajectories <strong>of</strong> pup's brain. Growth-associated protein-43 (GAP-43) is a presynaptic membrane phosphoprotein<br />
whose expression increases during developmental events such as axonal outgrowth or remodeling and synaptogenesis.<br />
Phosphorylation <strong>of</strong> GAP-43 by protein kinase C was correlated with enhanced axonal growth and transmitter release. In adult<br />
animals, increase <strong>of</strong> GAP-43 correlated with monoaminergic deficit in neuropsychiatric disorders. The present study examines<br />
the effects <strong>of</strong> repeated maternal restraint stress on the level <strong>of</strong> GAP-43 in the brain <strong>of</strong> rat pups. The results showed that<br />
prenatal stress significantly increased GAP-43 level in the PFC <strong>of</strong> rat pup during PND 7-14 as compared to control but not<br />
significant difference when observed at PND 21. Increased GAP-43 expression was also observed in the pup's hippocampus<br />
during the same postnatal periods. However, when observed at PND 60, pups born from stressed mother showed a significant<br />
lower (p < 0.001) GAP-43 expression as compare with control group. These changes indicate the direct effect <strong>of</strong> corticosteroid<br />
hormone, since repeated maternal injection with corticosterone (CORT, 40 mg/kg) during GD 14-21 also gave the same results.<br />
PND 7-14 is the peak period <strong>of</strong> synaptogenesis in these brain areas and abnormal axon sprouting and reorganization may lead<br />
to a defect in synaptic pruning at later stage <strong>of</strong> life. The results suggested that maternal stress is harmful to the developing<br />
brain and upregulation <strong>of</strong> GAP-43 indicated a protective mechanism against the toxicity <strong>of</strong> maternal stress hormone. Prenatal<br />
stress alter the normal developmental trajectories in the pup's brain may underlies the mechanism link between early life<br />
stress and neuropsychopathology in later life. © 2009 ISDN.<br />
No.468<br />
Author (s) : Jutapakdeegul, N.; Surakul, P.; Vanichviriyakit, R.; Weerachatyanukul, W.<br />
Title : Repeated carbenoxolone injection during late pregnancy decreased SPAR but increased Snk expression in the hippocampus<br />
<strong>of</strong> rat pups<br />
Source : INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE, Volume: 28, Issue: 8, Special Issue: SI, Pages: 686-686, DOI:<br />
10.1016/j.ijdevneu.<strong>2010</strong>.07.132, Published: DEC <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.469<br />
Author (s) : Kachadroka, S., Hall, A.M., Niedzielko, T.L., Chongthammakun, S., Floyd, C.L.<br />
Title : Effect <strong>of</strong> endogenous androgens on 17β-estradiol-mediated protection after spinal cord injury in male rats<br />
Source : Journal <strong>of</strong> Neurotrauma, Volume 27, Issue 3, 1 March <strong>2010</strong>, Pages 611-626<br />
Document Type : Article<br />
Keywords : Apoptosis; Bax; Bcl-xL; Estrogen; Serum levels<br />
<strong>Abstract</strong> : Several groups have recently shown that 17β-estradiol is protective in spinal cord injury (SCI). Testosterone can be<br />
aromatized to 17β-estradiol and may increase estrogen-mediated protection. Alternatively, testosterone has been shown to<br />
increase excitotoxicity in models <strong>of</strong> central nervous system (CNS) injury. These experiments test the hypothesis that<br />
endogenous testosterone in male rats alters 17β-estradiol-mediated protection by evaluating a delayed administration over a<br />
clinically relevant dose range and manipulating testicular-derived testosterone. Adult male Sprague Dawley rats were either<br />
gonadectomized or left gonad-intact prior to SCI. SCI was produced by a midthoracic crush injury. At 30min post SCI, animals<br />
received a subcutaneous pellet <strong>of</strong> 0.0, 0.05, 0.5, or 5.0mg <strong>of</strong> 17β-estradiol, released over 21 days. Hindlimb locomotion was<br />
analyzed weekly in the open field. Spinal cords were collected and analyzed for cell death, expression <strong>of</strong> Bcl-family proteins,<br />
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and white-matter sparing. Post-SCI administration <strong>of</strong> the 0.5- or 5.0-mg pellet improved hindlimb locomotion, reduced urinary<br />
bladder size, increased neuronal survival, reduced apoptosis, improved the Bax/Bcl-xL protein ratio, and increased whitematter<br />
sparing. In the absence <strong>of</strong> endogenous testicular-derived androgens, SCI induced greater apoptosis, yet 17β-estradiol<br />
administration reduced apoptosis to the same extent in gonadectomized and gonad-intact male rats. These data suggest that<br />
delayed post-SCI administration <strong>of</strong> a clinically relevant dose <strong>of</strong> 17β-estradiol is protective in male rats, and endogenous<br />
androgens do not alter estrogen-mediated protection. These data suggest that 17β-estradiol is an effective therapeutic<br />
intervention for reducing secondary damage after SCI in males, which could be readily translated to clinical trials. © <strong>2010</strong>,<br />
Mary Ann Liebert, Inc.<br />
No.470<br />
Author (s) : Kachaiyaphum, P., Howteerakul, N., Sujirarat, D., Siri, S., Suwannapong, N.<br />
Title : Serum cholinesterase levels <strong>of</strong> Thai chilli-farm workers exposed to chemical pesticides: Prevalence estimates and associated<br />
factors<br />
Source : Journal <strong>of</strong> Occupational Health, Volume 52, Issue 1, January <strong>2010</strong>, Pages 89-98<br />
Document Type : Article<br />
Keywords : Chemical pesticides; Chilli-farm workers; Pesticide-related symptoms; Serum cholinesterase; Thailand<br />
<strong>Abstract</strong> : Objective: To estimate the prevalence <strong>of</strong>, and factors associated with, abnormal serum cholinesterase (SChE) levels among<br />
chilli-farm workers in Chatturat District, Chaiyaphum Province. Methods: A total <strong>of</strong> 350 chilli-farm workers aged 18-60 yr were<br />
randomly sampled and interviewed. A reactive-paper finger-blood test was used to assess SChE levels. Results: The<br />
prevalence <strong>of</strong> abnormal SChE levels was 32.0%. The most common pesticide-related symptoms were dizziness (38.0%),<br />
headache (30.9%), nausea/vomiting (26.9%), and fever (26.9%). Multiple logistic regression analysis revealed 7 factors were<br />
independently associated with abnormal SChE level: male gender, single/separated/divorced, being a permanent worker,<br />
spraying pesticide more than 3 times per month, having moderate or poor pesticide-use behaviors, and low perceived<br />
susceptibility and severity <strong>of</strong> pesticide use. Conclusions: The prevalence <strong>of</strong> abnormal SChE levels among chilli-farm workers<br />
was quite high. It would be beneficial to decrease pesticide use and encourage alternative measures. Effective preventive<br />
interventions to increase correct perceptions <strong>of</strong> pesticide use, the use <strong>of</strong> personal protective measures and continuing<br />
monitoring for blood cholinesterase, especially for male permanent farm workers, are recommended.<br />
No.471<br />
Author (s) : Kaensaksiri, T.; Soontornchainaksaeng, P.; Soonthornchareonnon, N.; Prathanturarug, S.<br />
Title : Mutational breeding <strong>of</strong> Centella asiatica (L.) urban for medicinal purposes<br />
Source : PLANTA MEDICA, Volume: 76, Issue: 12, Pages: 1238-1238, Published: AUG <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.472<br />
Author (s) : Kaeoket, K., Chanapiwat, P., Tummaruk, P., Techakumphu, M.<br />
Title : Supplemental effect <strong>of</strong> varying L-cysteine concentrations on the quality <strong>of</strong> cryopreserved boar semen<br />
Source : Asian Journal <strong>of</strong> Andrology, Volume 12, Issue 5, September <strong>2010</strong>, Pages 760-765<br />
Document Type : Article<br />
Keywords : antioxidant; boar semen; cryopreservation; L-cysteine<br />
<strong>Abstract</strong> : Cryopreservation is associated with the production <strong>of</strong> reactive oxygen species, which leads to lipid peroxidation <strong>of</strong> the sperm<br />
membrane and consequently a reduction in sperm motility and decreased fertility potential. The aim <strong>of</strong> this study was to<br />
determine the optimal concentration <strong>of</strong> L-cysteine needed for cryopreservation <strong>of</strong> boar semen. Twelve boars provided<br />
semen <strong>of</strong> proven motility and morphology for this study. The semen was divided into four portions in which the lactose-egg<br />
yolk (LEY) extender used to resuspend the centrifuged sperm pellet was supplemented with various concentrations <strong>of</strong> Lcysteine<br />
to reach 0 mmol L 1 (group I, control), 5 mmol L 1 (group II), 10 mmol L 1 (group III) and 15 mmol L 1 (group IV).<br />
Semen suspensions were loaded in straws (0.5 mL) and placed in a controlled-rate freezer. After cryopreservation, frozen<br />
semen samples were thawed and investigated for progressive motility, viability using SYBR-14/EthD-1 staining and acrosome<br />
integrity using FITC-PNA/EthD-1 staining. There was a significantly higher (P
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
a biphasic effect <strong>of</strong> L-cysteine, with the highest percentage <strong>of</strong> progressive motility, viability and acrosomal integrity in group III.<br />
In conclusion, 5 or 10 mmol L 1 was the optimum concentration <strong>of</strong> L-cysteine to be added to the LEY extender for improving<br />
the quality <strong>of</strong> frozen-thawed boar semen. © <strong>2010</strong> AJA, SIMM & SJTU All rights reserved.<br />
No.473<br />
Author (s) : Kaeoket, K., Sang-urai, P., Thamniyom, A., Chanapiwat, P., Techakumphu, M.<br />
Title : Effect <strong>of</strong> docosahexaenoic acid on quality <strong>of</strong> cryopreserved boar semen in different breeds<br />
Source : Reproduction in Domestic Animals, Volume 45, Issue 3, June <strong>2010</strong>, Pages 458-463<br />
Document Type : Article<br />
Keywords : docosahexaenoic acid, fish oil; acrosome, animal, animal disease, article, cell membrane, cell survival, congenital<br />
malformation, cryopreservation, male, methodology, physiology, species difference, sperm, sperm<br />
preservation, spermatozoon, spermatozoon motility, swine, ultrastructure; Acrosome, Animals, Cell Membrane, Cell<br />
Survival, Cryopreservation, Docosahexaenoic Acids, Fish Oils, Male, Semen, Semen Preservation, Species Specificity, Sperm<br />
Motility, Spermatozoa, Swine; Suidae<br />
<strong>Abstract</strong> : Contents: During the cryopreservation process, the level <strong>of</strong> polyunsaturated fatty acids, especially docosahexaenoic acid<br />
(DHA), in the sperm plasma membrane decreases significantly because <strong>of</strong> lipid peroxidation, which may contribute to sperm<br />
loss quality (i.e. fertility) <strong>of</strong> frozen-thawed semen. The aim <strong>of</strong> this study was to investigate the effect <strong>of</strong> supplementation <strong>of</strong><br />
DHA (fish oil) in freezing extender II on frozen-thawed semen quality. Semen from 20 boars <strong>of</strong> proven motility and<br />
morphology, were used in this study. Boar semen was split into four groups, in which the lactose-egg yolk (LEY) extender used<br />
to resuspend the centrifuged sperm pellet was supplemented with various levels <strong>of</strong> fish oil to reach DHA level <strong>of</strong> 1X (group I,<br />
control, no added fish oil), 6X (group II), 12X (group III) and 18X (group IV). Semen solutions were frozen by using a controlled<br />
rate freezer. After cryopreservation, frozen semen was thawed and evaluated for progressive motility, viability by using SYBR-<br />
14/Ethidiumhomodimer-1 (EthD-1) staining and acrosome integrity by using FITC-PNA/EthD-1 staining. There was a significantly<br />
higher (p. < 0.001) percentage <strong>of</strong> progressive motility, viability and acrosome integrity in DHA (fish oil) supplemented groups<br />
than control group. Generally, there seemed to be a dose-dependent effect <strong>of</strong> DHA, with the highest percentage <strong>of</strong><br />
progressive motility, viability and acrosome integrity in group-III. In conclusion, supplementation <strong>of</strong> the LEY extender with DHA<br />
by adding fish oil was effective for freezing boar semen as it resulted in higher post-thaw plasma membrane integrity and<br />
progressive motility. © 2008 The Authors. Journal compilation © 2008 Blackwell Verlag.<br />
No.474<br />
Author (s) : Kaeoket, K., Srisowanna, T., Wichaidit, U., Chanapiwat, P., Manee-in, S.<br />
Title : Comparative study on six different long term commercial extenders for fresh boar semen<br />
Source : Thai Journal <strong>of</strong> Veterinary Medicine, Volume 40, Issue 3, <strong>2010</strong>, Pages 257-263<br />
Document Type : Article<br />
Keywords : Acrosome integrity; Boar; Long term extenders; Motility; Viability<br />
<strong>Abstract</strong> : In Thailand, there are numerous commercial extenders for preservation <strong>of</strong> boar semen; however, no study has been carried<br />
out to compare these extenders in view <strong>of</strong> maintaining <strong>of</strong> sperm motility, plasma membrane integrity and acrosome integrity<br />
during cold storage. The aim <strong>of</strong> this study was to assess the percentage <strong>of</strong> sperm motility, HOST positive, viability and<br />
acrosome integrity <strong>of</strong> boar spermatozoa extended in Merck-III, Androstar®Plus, ModenaTM, NUTRIXcell®, VITASEM LD,<br />
Duragen and D<strong>of</strong>u goldTM. Ejaculated from boars (n=6, one ejaculate from each boar) were collected and sub-samples<br />
(splited samples) were diluted (3 × 109 spermatozoa/100 ml) in the different extenders and stored for 10 days at 18°C. On<br />
every second day (days 0, 2, 4, 6, 8, 10) after storage, the sperm parameters such as sperm motility, HOST positive, viability<br />
and acrosome integrity were evaluated. There were significant differences in characteristic <strong>of</strong> sperm motility and acrosome<br />
integrity between extenders. For instance, on day 4, Merck III (short term extender) and other long term extenders are able to<br />
maintain the semen qualities as claimed by the manufacturing. On day 8, the percentages <strong>of</strong> sperm motility, viability and<br />
acrosome integrity were highest (p< 0.01) in Androstar®Plus (72%) and ModenaTM (75%), Androstar®Plus (67%) and<br />
ModenaTM (65.6%), and Androstar®Plus (74.6%), Duragen (72.4%) and VITASEM LD (71.6%), respectively. In conclusion,<br />
changes in motility, viability and acrosome integrity during storage were affected by the extender utilized, however long term<br />
extenders maintained a high percentage (70%) <strong>of</strong> sperm motility (i.e. Androstar®Plus, ModenaTM and Duragen) and acrosome<br />
integrity (i.e. Androstar®Plus, VITASEM LD and Duragen) through 8 days <strong>of</strong> storage.<br />
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No.475<br />
Author (s) : Kaewboonchoo, O., Morioka, I., Saleekul, S., Miyai, N., Chaikittiporn, C., Kawai, T.<br />
Title : Blood lead level and cardiovascular risk factors among bus drivers in Bangkok, Thailand<br />
Source : Industrial Health, Volume 48, Issue 1, January <strong>2010</strong>, Pages 61-65<br />
Document Type : Article<br />
Keywords : Blood lead; Bus drivers; Cardiovascular system; Risk factor; SDPTG<br />
<strong>Abstract</strong> : This study aimed to clarify the role <strong>of</strong> blood lead level (Pb-B) as one <strong>of</strong> the cardiovascular risk factors. To evaluate the<br />
cardiovascular risk the second derivative finger photoplethysmogram (SDPTG) was used. The subjects comprised <strong>of</strong> 420 male<br />
bus drivers in Thailand. The subjects' age ranged from 20 to 60 yr. Mean age (± standard deviation) were 41.6 (± 7.7) yr. Mean<br />
working years was 8.8 (± 6.8) yr. Pb-B ranged from 2.5 to 16.2 μg/dl with the mean Pb-B <strong>of</strong> 6.3 (± 2.2) μg/dl. The mean <strong>of</strong><br />
aging index <strong>of</strong> SDPTG (SDPTG-AI) were -0.50 (± 0.30). The SDPTG-AI increases with age, Pb-B, smoking and alcohol<br />
consumption. There was significant correlation between Pb-B and SDPTG-AI after controlling for age, body mass index and<br />
lifestyle factors. These results suggest that Pb-B is possibly an independent cardiovascular risk factor for bus drivers exposed<br />
to lower level <strong>of</strong> lead.<br />
No.476<br />
Author (s) : Kaewkhomdee, N., Mounicou, S., Szpunar, J., Lobinski, R., Shiowatana, J.<br />
Title : Characterization <strong>of</strong> binding and bioaccessibility <strong>of</strong> Cr in Cr-enriched yeast by sequential extraction followed by twodimensional<br />
liquid chromatography with mass spectrometric detection<br />
Source : Analytical and Bioanalytical Chemistry, Volume 396, Issue 3, <strong>2010</strong>, Pages 1355-1364<br />
Document Type : Article<br />
Keywords : Chromium; Speciation; Yeast<br />
<strong>Abstract</strong> : Sequential extraction (water, Driselase, protease XIV) and extraction with simulated gastric and intestinal fluids were proposed<br />
to characterize the binding and the bioaccessibility <strong>of</strong> chromium in two commercial food supplements obtained by<br />
incorporation <strong>of</strong> this element into yeast. Chromium in Cr-enriched yeast was found to be hardly extractable with water,<br />
Driselase, or simulated gastric fluid (recoveries <strong>of</strong> approximately 10-20%), but proteolysis or gastrointestinal fluid digestion<br />
released more than half <strong>of</strong> the chromium present. Fractionation with size-exclusion chromatography with Cr-specific<br />
detection by inductively coupled plasma mass spectrometry (ICP MS) allowed the distinction <strong>of</strong> two fractions: one below<br />
approximately 1 kDa and one 1-5 kDa; they contained the entirety <strong>of</strong> the released Cr with proportions varying as a function <strong>of</strong><br />
the extracting solution and the origin <strong>of</strong> sample. When collected and investigated by reversed-phase high-performance liquid<br />
chromatography-ICP MS, the low molecular mass fraction was found to release Cr(III), whereas the heavier one showed most<br />
<strong>of</strong> Cr bound in fairly stable hydrophobic complexes. However, an attempt <strong>of</strong> their identification by electrospray ionization<br />
MS/MS and matrix-assisted laser desorption ionization MS was not successful. © 2009 Springer-Verlag.<br />
No.477<br />
Author (s) : Kaewkhong, K., Mazzolini, A., Emarat, N., Arayathanitkul, K.<br />
Title : Thai high-school students' misconceptions about and models <strong>of</strong> light refraction through a planar surface<br />
Source : Physics Education, Volume 45, Issue 1, <strong>2010</strong>, Article number 012, Pages 97-107<br />
Document Type : Article<br />
Keywords : –<br />
<strong>Abstract</strong> : This article investigates the optics misconceptions <strong>of</strong> 220 year 11 Thai high-school students. These misconceptions became<br />
apparent when the students attempted to explain how an object submerged in a water tank is 'seen' by an observer looking<br />
into the tank from above and at an angle. The two diagnostic questions used in the study probe the students' ability to use a<br />
ray diagram to explain the relationship between object, image and observer, and then to use the ray diagram to qualitatively<br />
determine the position <strong>of</strong> the image. The study indicates that these high-school students, even after instruction, had<br />
significant misconceptions about the direction <strong>of</strong> propagation <strong>of</strong> light, how light refracts at an interface, and how to determine<br />
the position <strong>of</strong> an image. The study revealed that students used various concept models to explain how the object can be<br />
'seen' in this situation. Only 22% <strong>of</strong> all students had a qualitative understanding <strong>of</strong> how to use a ray diagram to determine<br />
image position, and only 1 <strong>of</strong> 220 students could identify the correct image position using correct reasoning. Our results<br />
indicate that students require very careful instruction if they are to understand how objects are 'seen' and how images are<br />
formed when light refracts through a planar surface. © <strong>2010</strong> IOP Publishing Ltd.<br />
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No.478<br />
Author (s) : Kaewkungwal, J., Singhasivanon, P., Khamsiriwatchara, A., Sawang, S., Meankaew, P., Wechsart, A.<br />
Title : Application <strong>of</strong> smart phone in "better Border Healthcare Program": A module for mother and child care<br />
Source : BMC Medical Informatics and Decision Making, Volume 10, Issue 1, <strong>2010</strong>, Article number 69<br />
Document Type : Article<br />
Keywords : –<br />
<strong>Abstract</strong> : Background. To assess the application <strong>of</strong> cell phone integrating into the healthcare system to improve antenatal care (ANC)<br />
and expanded programme on immunization (EPI) services for the under-served population in border area. Methods. A module<br />
combining web-based and mobile technology was developed to generate ANC/EPI visit schedule dates in which the<br />
healthcare personnel can cross-check, identify and update the mother's ANC and child's EPI status at the healthcare facility or<br />
at the household location when performing home visit; with additional feature <strong>of</strong> sending appointment reminder directly to<br />
the scheduled mother in the community. Results. The module improved ANC/EPI coverage in the study area along the<br />
country border including for both Thai and non-Thai mothers and children who were either permanent resident or migrants;<br />
numbers <strong>of</strong> ANC and EPI visit on-time as per schedule significantly increased; there was less delay <strong>of</strong> antenatal visits and<br />
immunizations. Conclusions. The module integrated and functioned successfully as part <strong>of</strong> the healthcare system; it is proved<br />
for its feasibility and the extent to which community healthcare personnel in the low resource setting could efficiently utilize<br />
it to perform their duties. © <strong>2010</strong> Kaewkungwal et al; licensee BioMed Central Ltd.<br />
No.479<br />
Author (s) : Kaewkungwal, J.; Pitisuttithum, P.; Nitayapan, S.; Stablein, D.; Chiu, J.; Benenson, M.; Kim, J.; Robb, M.; Michael, N.; Rerks-Ngarm,<br />
S.<br />
Title : Exploratory Age-stratified Analysis <strong>of</strong> Risk-taking Behaviors and Trial Participation Outcomes in the Thai Phase III HIV Vaccine<br />
Trial<br />
Source : AIDS RESEARCH AND HUMAN RETROVIRUSES, Volume: 26, Issue: 10, Pages: A9-A9, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.480<br />
Author (s) : Kaewphinit, T., Santiwatanakul, S., Promptmas, C., Chansiri, K.<br />
Title : Detection <strong>of</strong> non-amplified Mycobacterium tuberculosis genomic DNA using piezoelectric DNA-based biosensors<br />
Source : Sensors, Volume 10, Issue 3, March <strong>2010</strong>, Pages 1846-1858<br />
Document Type : Article<br />
Keywords : Mycobacterium tuberculosis; Non-amplified genomic DNA; Piezoelectric biosensor<br />
<strong>Abstract</strong> : Piezoelectric DNA-based biosensor technology was developed as a new method for detection <strong>of</strong> M. tuberculosis. This<br />
method consists <strong>of</strong> immobilizing a thiol-modified oligonucleotide probe on the gold electrode surface <strong>of</strong> a quartz crystal,<br />
using a selfassembled monolayer method. The advantage <strong>of</strong> this study is that a non-amplified genomic bacterial DNA target<br />
was used. Instead, the genomic DNA was digested by restriction enzyme to obtain DNA fragments containing the target<br />
sequence. The fabricated biosensor was evaluated through an examination <strong>of</strong> 200 samples. No cross hybridization were<br />
observed against M. avium complex and other microorganisms. This target DNA preparation, without PCR amplification, will<br />
reduce time, costs, and the tedious step <strong>of</strong> amplification. © <strong>2010</strong> by the authors.<br />
No.481<br />
Author (s) : Kaewpongsri, S., Sukasem, C., Srichunrusami, C., Pasomsub, E., Zwang, J., Pairoj, W., Chantratita, W.<br />
Title : An integrated bioinformatics approach to the characterization <strong>of</strong> influenza A/H5N1 viral sequences by microarray data:<br />
Implication for monitoring H5N1 emerging strains and designing appropriate influenza vaccines<br />
Source : Molecular and Cellular Probes, Volume 24, Issue 6, December <strong>2010</strong>, Pages 387-395<br />
Document Type : Article<br />
Keywords : Avian influenza; H5N1 virus; Microarray; Semi-conducted-based oligonucleotide<br />
<strong>Abstract</strong> : In order to characterize A/H5N1 viral sequences, a bioinformatics approach accurately identified viral sequences from<br />
discovery <strong>of</strong> a sequence signature, which provided enough distinctive information for sequence identification. Eight highly<br />
pathogenic H5N1 viral isolations were collected from different areas <strong>of</strong> Thailand between 2003 and 2006, and were used for<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
analysis <strong>of</strong> H5N1 genotypic testing with a semiconductor-based oligonucleotide microarray. All H5N1 samples and H1N1, H4N8<br />
negative controls were correctly subtyped. Sensitivity <strong>of</strong> the eight oligonucleotide probes, with optimized cut-<strong>of</strong>fs, ranged<br />
from 70% (95% CI 65-75) to 87% (95% CI 84-91), and the corresponding Kappa values ranged from 0.76 (95% CI 0.72-0.80) to<br />
0.86 (95% CI 0.83-0.89). Semi-conductor-based oligonucleotide array and oligonucleotide probes corresponded well when<br />
detecting H5N1. After fully correcting the subtype from the result <strong>of</strong> microarray signal intensity, the microarray output method<br />
combined with bioinformatics tools, identified and monitored genetic variations <strong>of</strong> H5N1. Capability <strong>of</strong> distinguishing different<br />
strains <strong>of</strong> H5N1 from Thailand was the outstanding feature <strong>of</strong> this assay. Ninety percent <strong>of</strong> HA and NA (4/5) genes were<br />
sequenced correctly, in accordance with previous examinations performed by classical diagnostic methods. The low-mediumhigh<br />
bioinformatics resolutions were able to predict an epidemic strain <strong>of</strong> H5N1. This study also showed the advantage <strong>of</strong><br />
using a large genotypic database to predict the epidemic strain <strong>of</strong> H5N1. However, the monitoring protocol <strong>of</strong> this new strain<br />
has been recommended for further study with a large-scale sample. © <strong>2010</strong> Elsevier Ltd.<br />
No.482<br />
Author (s) : Kaewsaneha, C., Opaprakasit, P., Polpanich, D., Smanmoo, S., Tangboriboonrat, P.<br />
Title : Composite particles <strong>of</strong> disinfectant nanocapsules-skim rubber latex<br />
Source : <strong>International</strong> Journal <strong>of</strong> Polymer Analysis and Characterization, Volume 15, Issue 8, November <strong>2010</strong>, Pages 524-535<br />
Document Type : Article<br />
Keywords : Composites; Core-shell polymers; Nanoparticle; Rubber; Surfactants<br />
<strong>Abstract</strong> : Skim latex, a by-product <strong>of</strong> natural rubber latex, was prevulcanized by sulfur and then used in the preparation <strong>of</strong> composite<br />
particles with disinfectant nanocapsules. Each polymeric nanocapsule contained chlorhexidine digluconate (CHD) as a<br />
disinfectant agent encapsulated with poly(methyl acrylate) (PMA) as a shell. The driving force for the heterocoagulation <strong>of</strong><br />
CHD-PMA nanocapsule and sulfur-prevulcanized skim (SPVS) particle was the electrostatic interaction between the positive<br />
charge <strong>of</strong> lecithin residing on the surface <strong>of</strong> the nanocapsule and the negative charge from the indigenous surfactant (proteinlipid)<br />
and/or from sodium dodecyl sulfate on the surface <strong>of</strong> the SPVS latex particle. The zeta potential and morphology <strong>of</strong><br />
heterocoagulated particles indicated the formation <strong>of</strong> the CHD-PM A/SPVS composite particles. Structures and formation<br />
mechanisms <strong>of</strong> the composite particles with different blend ratios were revealed by depth pr<strong>of</strong>iling confocal Raman spectra.<br />
© Taylor & Francis Group, LLC.<br />
No.483<br />
Author (s) : Kaewsaneha, C., Tangboriboonrat, P., Polpanich, D., Smanmoo, S.<br />
Title : Preparation <strong>of</strong> core-shell particle <strong>of</strong> disinfectant agent nanocapsules-skim rubber particles by the heterocoagulation<br />
technique<br />
Source : Macromolecular Research, Volume 18, Issue 9, September <strong>2010</strong>, Pages 876-883<br />
Document Type : Article<br />
Keywords : Capsule; Composite; Heterocoagulation; Nanoparticle; Skim<br />
<strong>Abstract</strong> : Sulphur-prevulcanised skim (SPVS) rubber particles were heterocoagulated on an aggregate <strong>of</strong> a disinfectant agent<br />
(chlorhexidine digluconate; CHD)-poly(methyl acrylate) (PMA) nanocapsules. The CHD-PMA nanocapsule stabilized by lecithin<br />
was prepared using the modified nanoprecipitation <strong>of</strong> PMA from an organic continuous phase onto nanodroplet containing<br />
the CHD aqueous solution. Cationic and anionic nanocapsules were produced by separately redispersing the nanocapsules<br />
into a water and 0.5% sodium dodecyl sulphate (SDS) aqueous solution, respectively. The largest aggregate, which was<br />
obtained from homocoagulation <strong>of</strong> the CHD-PMA nanocapsules with opposite charges, was selected as a core surrounded<br />
with SPVS shell particles. An interpolymer complex between poly(ethylene oxide) (PEO) moieties adsorbed on the aggregate<br />
and indigenous surfactant (protein- lipid) and/or SDS on the SPVS particle surface was the driving force for heterocoagulation.<br />
Data from the size, zeta potential measurements and TEM images <strong>of</strong> the heterocoagulated CHD-PMA/SPVS indicated the<br />
composite particle containing the CHD-PMA core.<br />
No.484<br />
Author (s) : Kaewsuwan, S., Bunyapraphatsara, N., Cove, D.J., Quatrano, R.S., Chodok, P.<br />
Title : High level production <strong>of</strong> adrenic acid in Physcomitrella patens using the algae Pavlova sp. ∆5-elongase gene<br />
Source : Bioresource Technology, Volume 101, Issue 11, June <strong>2010</strong>, Pages 4081-4088<br />
Document Type : Article<br />
Keywords : ∆5-Elongase; Adrenic acid; Pavlova sp.; Physcomitrella patens; Response surface methodology<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : Adrenic acid (ADA), an ω-6 polyunsaturated fatty acid (PUFA), has attracted much interest due to its pharmaceutical<br />
potential. Exploiting the wealth <strong>of</strong> information currently available on in planta oil biosynthesis, and coupling this information<br />
with the tool <strong>of</strong> genetic engineering, it is now feasible to deliberately alter fatty acid biosynthetic pathways to generate<br />
unique oils in commodity crops. In this study, a ∆5-elongase gene from the algae Pavlova sp. related to the biosynthesis <strong>of</strong><br />
C22 PUFAs was targeted to enable production <strong>of</strong> ADA in the moss Physcomitrella patens. Heterologous expression <strong>of</strong> this<br />
gene was under the control <strong>of</strong> a tandemly duplicate 35S promoter. It was established that ADA (0.42 mg/l) was synthesized in<br />
P. patens from endogenous arachidonic acid (ARA) via the expressed Pavlova sp. ∆5-elongase in the moss. In an attempt to<br />
maximize ADA production, medium optimization was effected by the response surface methodology (RSM), resulting in a<br />
significant elevation <strong>of</strong> ADA (4.51 mg/l) production under optimum conditions. To the best <strong>of</strong> our knowledge, this is the first<br />
study describing the expression <strong>of</strong> a PUFA synthesizing enzyme in non-seed lower plant without supplying the exogenous<br />
fatty acid. © <strong>2010</strong> Elsevier Ltd. All rights reserved.<br />
No.485<br />
Author (s) : Kalong, M., Ngamsuriyaroj, S., Visoottiviseth, V.<br />
Title : Dynamic key management for secure continuous hand<strong>of</strong>f in wireless LAN<br />
Source : <strong>2010</strong> 6th IEEE Workshop on Secure Network Protocols, NPSec <strong>2010</strong>, <strong>2010</strong>, Article number 5634455, Pages 7-12<br />
Document Type : Conference Paper<br />
Keywords : IEEE802.11i; Key distribution; Key management; Secure roaming; Wireless security<br />
<strong>Abstract</strong> : The secure dynamic key management has been introduced in the wireless security standard, IEEE802.11i. However, dynamic<br />
keys are very limited and their lifetime is very short. In addition, those keys must be initialized by a central authority before<br />
sending them to all communicating hosts, and the key must be renewed after every single use. This paper proposes a novel<br />
dynamic key management mechanism in wireless networks for fast authentication and secure continuous roaming. Our work<br />
focuses on generating session keys independently after the first pair-wise session key has been established between a mobile<br />
node and the home access point. The OMNet++ network simulator is used for performance evaluation. The roaming<br />
performance is measured using three scenarios having different parameters including moving speed, background workload,<br />
and client density. The experimental results show that our proposed work outperforms the standard IEEE802.11i and gives<br />
high scalability as well. ©<strong>2010</strong> IEEE.<br />
No.486<br />
Author (s) : Kalpravidh, R.W., Siritanaratkul, N., Insain, P., Charoensakdi, R., Panichkul, N., Hatairaktham, S., Srichairatanakool, S.,<br />
Phisalaphong, C., Rachmilewitz, E., Fucharoen, S.<br />
Title : Improvement in oxidative stress and antioxidant parameters in β-thalassemia/Hb E patients treated with curcuminoids<br />
Source : Clinical Biochemistry, Volume 43, Issue 4-5, March <strong>2010</strong>, Pages 424-429<br />
Document Type : Article<br />
Keywords : β-thalassemia/Hb E; Antioxidant; Antioxidant enzymes; Curcuminoids; Oxidative stress<br />
<strong>Abstract</strong> : Objectives: To evaluate the hematological pr<strong>of</strong>ile, oxidative stress, and antioxidant parameters in β-thalassemia/Hb E patients<br />
treated with curcuminoids for 12 months. Design and methods: Twenty-one β-thalassemia/Hb E patients were given 2<br />
capsules <strong>of</strong> 250 mg each <strong>of</strong> curcuminoids (a total <strong>of</strong> 500 mg) daily for 12 months. Blood was collected every 2 months during<br />
treatment and 3 months after withdrawal and was determined for complete blood count, malonyldialdehyde (MDA),<br />
superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), reduced glutathione (GSH) in red blood cells (RBC), and nontransferrin<br />
bound iron (NTBI) in serum. Results: The increased oxidative stress in β-thalassemia/Hb E patients was shown by<br />
higher levels <strong>of</strong> MDA, SOD, GSH-Px in RBC, serum NTBI, and lower level <strong>of</strong> RBC GSH. Curcuminoids administration resulted in<br />
improvement <strong>of</strong> all the measured parameters as long as they were administered. After 3 months withdrawal <strong>of</strong> treatment, all<br />
parameters returned close to baseline levels. Conclusion: Curcuminoids may be used to ameliorate oxidative damage in<br />
patients with β-thalassemia/Hb E disease. © 2009 The Canadian Society <strong>of</strong> Clinical Chemists.<br />
No.487<br />
Author (s) : Kamanamool, N., McEvoy, M., Attia, J., Ingsathit, A., Ngamjanyaporn, P., Thakkinstian, A.<br />
Title : Efficacy and adverse events <strong>of</strong> mycophenolate m<strong>of</strong>etil versus cyclophosphamide for induction therapy <strong>of</strong> lupus nephritis:<br />
Systematic review and meta-analysis<br />
Source : Medicine, Volume 89, Issue 4, July <strong>2010</strong>, Pages 227-235<br />
Document Type : Review<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : albumin, corticosteroid, creatinine, cyclophosphamide, mycophenolic acid 2 morpholinoethyl ester; clinical trial, controlled<br />
clinical trial, creatinine blood level, drug efficacy, drug safety, drug withdrawal, gastrointestinal<br />
symptom, human, infection, kidney function, leukopenia, lupus erythematosus nephritis, meta analysis, priority<br />
journal, protein urine level, randomized controlled trial, remission, review, systematic review, treatment outcome;<br />
Cyclophosphamide, Humans, Immunosuppressive Agents, Lupus Nephritis, Mycophenolic Acid, Randomized Controlled Trials<br />
as Topic, Risk Assessment, Treatment Outcome<br />
<strong>Abstract</strong> : We performed a systematic review and meta-analysis <strong>of</strong> randomized controlled trials to compare complete remission and<br />
adverse events (that is, infection, leukopenia, and gastrointestinal [GI] symptoms) between mycophenolate m<strong>of</strong>etil (MMF) and<br />
cyclophosphamide (CYC) for the treatment <strong>of</strong> lupus nephritis (LN). We identified trials from MEDLINE using the PubMed and<br />
Ovid search engines, and from The Cochrane Central Register <strong>of</strong> Randomized Controlled Trials. Eligible studies were<br />
randomized controlled trials comparing MMF with CYC with 1 <strong>of</strong> following outcomes: complete remission, complete/partial<br />
remission, infection, leukopenia, GI symptoms, serum creatinine, 24-hour urine protein, and urine albumin. Data were<br />
independently extracted by 2 reviewers. Five trials with a total <strong>of</strong> 638 patients were eligible for review. While the MMF group<br />
tended to achieve complete remission more frequently than the CYC group, this was not significant (pooled risk ratio [RR],<br />
1.60; 95% confidence interval [CI], 0.87-2.93). Pooling based on the 4 homogeneous trials yielded similar results-that is, no<br />
benefit <strong>of</strong> MMF compared with CYC groups (RR, 1.15; 95% CI, 0.74-1.77). The complete or partial remission rates were also not<br />
different (pooled RR, 1.21; 95% CI, 0.97-1.48) among the groups. The adverse events (infection, renal function, and GI<br />
symptoms) were not significantly different, except for leukopenia, which was lower in the MMF group.In summary, patients<br />
treated with MMF and CYC had similar remission rates, but the MMF group had less frequent leukopenia than the CYC group.<br />
Further large-scale trials are needed to confirm these results. © <strong>2010</strong> by Lippincott Williams & Wilkins.<br />
No.488<br />
Author (s) : Kambutong, S., Kuhakarn, C., Tuchinda, P., Pohmakotr, M.<br />
Title : Synthesis <strong>of</strong> (+)-4-Desoxypentenomycin and Analogues<br />
Source : SYNTHESIS-STUTTGART, Issue: 9, Pages: 1453-1458, DOI: 10.1055/s-0029-1218690, Published: MAY <strong>2010</strong><br />
Document Type : Article<br />
Keywords : oxygenated cyclopentenones; desoxycyclopentenones; alpha-sulfinyl carbanions; cyclizations; asymmetric synthesis<br />
<strong>Abstract</strong> : A synthesis <strong>of</strong> (+)-4-desoxypentenomycin is reported here; it involves diastereoselective phenylsulfanylpropylation <strong>of</strong> an<br />
enolate anion derived from methyl (2R,5R,6R)-5,6-dimethoxy-5,6-dimethyl[1,4]dioxane-2-carboxylate, obtained from Dmannitol,<br />
and is followed by sulfide oxidation, intramolecular acylation <strong>of</strong> the alpha-sulfinyl carbanion, sulfoxide elimination,<br />
and hydrolysis. Straightforward access to substituted analogues <strong>of</strong> (+)-4-desoxypentenomycin was also demonstrated by<br />
means <strong>of</strong> Suzuki-Miyaura, Sonogashira, and Heck coupling reactions.<br />
No.489<br />
Author (s) : Kamlangkla, K., Paosawatyanyong, B., Pavarajarn, V., Hodak, J.H., Hodak, S.K.<br />
Title : Mechanical strength and hydrophobicity <strong>of</strong> cotton fabric after SF6 plasma treatment<br />
Source : Applied Surface Science, Volume 256, Issue 20, 1 August <strong>2010</strong>, Pages 5888-5897<br />
Document Type : Article<br />
Keywords : Cotton; Mechanical properties; Plasma processing<br />
<strong>Abstract</strong> : Surface treatments to tailor fabric properties are in high demand by the modern garment industry. We studied the effect <strong>of</strong><br />
radio-frequency inductively coupled SF6 plasma on the surface characteristics <strong>of</strong> cotton fabric. The duration <strong>of</strong> the treatment<br />
and the SF6 pressure were varied systematically. We measured the hydrophobicity <strong>of</strong> treated cotton as a function <strong>of</strong> storage<br />
time and washing cycles. We used the weight loss (%) along with the etching rate, the tensile strength, the morphology<br />
changes and the hydrophobicity <strong>of</strong> the fabric as observables after treatments with different plasma conditions. The weight<br />
loss remains below 1% but it significantly increases when the treatment time is longer than 5 min. Substantial changes in the<br />
surface morphology <strong>of</strong> the fiber are concomitant with the increased etching rate and increased weight loss with measurable<br />
consequences in their mechanical characteristics. The measured water absorption time reaches the maximum <strong>of</strong> 210 min<br />
when the SF6 pressure is higher than 0.3 Torr. The water contact angle (149 °) and the absorption time (210 min) <strong>of</strong> cotton<br />
treated with extreme conditions appear to be durable as long as the fabric is not washed. X-ray photoelectron spectroscopy<br />
analysis reveals that the water absorption time <strong>of</strong> the fabric follows the same increasing trend as the fluorine/carbon ratio at<br />
the fabric surface and atom density <strong>of</strong> fluorine measured by Ar actinometer. © <strong>2010</strong> Elsevier B.V. All rights reserved.<br />
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No.490<br />
Author (s) : Kamphunthong, W., Sirisinha, K.<br />
Title : Thermal property improvement <strong>of</strong> ethylene-octene copolymer through the combined introduction <strong>of</strong> filler and silane<br />
crosslink<br />
Source : Journal <strong>of</strong> Applied Polymer Science, Volume 115, Issue 1, 5 January <strong>2010</strong>, Pages 424-430<br />
Document Type : Article<br />
Keywords : Composites; Crosslinking; Mechanical properties; Thermal properties<br />
<strong>Abstract</strong> : In many applications, e.g., wire and cable insulation, hot water pipe, high-temperature properties <strong>of</strong> polymer are essential.<br />
This article presents the use <strong>of</strong> silane crosslinking together with the addition <strong>of</strong> particular filler in improving the thermal and<br />
mechanical properties <strong>of</strong> ethylene-octene copolymer (EOC). The effects <strong>of</strong> filler surface characteristics on siloxane network<br />
structure developed and final properties <strong>of</strong> the crosslinked products are discussed. The results show an increase in the<br />
decomposition temperature <strong>of</strong> EOC more than 50°C after modification. Only crosslinked composites are able to withstand the<br />
hightemperature environment <strong>of</strong> aging test which is beyond the melting temperature <strong>of</strong> the matrix polymer. The crosslinked<br />
composites filled with calcium carbonate show superior properties to those with silica, due to a higher crosslink density and<br />
tighter network structure formed. The silane coupling mechanism and the presence <strong>of</strong> bound polymer on silica surfaces<br />
cause difficulties for the crosslink formation in the silica filled systems. However, an advantageous influence <strong>of</strong> both silane<br />
coupling and crosslink reaction in the silica filled composites is seen on the enhanced tensile strength and modulus <strong>of</strong> the<br />
materials. © 2009 Wiley Periodicals, Inc.<br />
No.491<br />
Author (s) : Kananthai, A., Bunpog, C.<br />
Title : On the delta hedging related to the eigenvalues and the interest rate <strong>of</strong> the black-scholes equation<br />
Source : <strong>International</strong> Journal <strong>of</strong> Pure and Applied Mathematics, Volume 65, Issue 4, <strong>2010</strong>, Pages 489-500<br />
Document Type : Article<br />
Keywords : Black-Scholes equation; Delta hedging<br />
<strong>Abstract</strong> : In this article, we study the delta hedging which is another way <strong>of</strong> minimizing the risk <strong>of</strong> investment. We can relate the delta<br />
hedging to the eigenvalues and the interest rate <strong>of</strong> the Black-Scholes equation. We found that such delta hedging depending<br />
on the relationship between the eigenvalues and the interest rate. We also found that the asymptotic form <strong>of</strong> the delta<br />
hedging related to the price <strong>of</strong> stock. Moreover, the results <strong>of</strong> this paper may not be useful in the real world application. But<br />
at least this paper may create the new results in the mathematical area which applying in the Financial Mathematics. © <strong>2010</strong><br />
Academic <strong>Publications</strong>.<br />
No.492<br />
Author (s) : Kang, C.-I., Song, J.-H., Chung, D.R., Peck, K.R., Ko, K.S., Yeom, J.-S., Kim, S.-W., Chang, H.-H., Kim, Y.-S., Jung, S.-I., Son, J.S.,<br />
Hsueh, P.-R., So, T.M.K., Lalitha, M.K., Yang, Y., Huang, S.-G., Wang, H., Lu, Q., Carlos, C.C., Perera, J.A., Chiu, C.-H., Liu, J.-W.,<br />
Chongthaleong, A., Thamlikitkul, V., Van Pham, H.<br />
Title : Clinical impact <strong>of</strong> methicillin resistance on outcome <strong>of</strong> patients with Staphylococcus aureus infection: A stratified analysis<br />
according to underlying diseases and sites <strong>of</strong> infection in a large prospective cohort<br />
Source : Journal <strong>of</strong> Infection, Volume 61, Issue 4, October <strong>2010</strong>, Pages 299-306<br />
Document Type : Article<br />
Keywords : Infection; Methicillin resistance; Risk factors; Staphylococcus aureus; Treatment outcome<br />
<strong>Abstract</strong> : Objective: This study was conducted to identify the predictors <strong>of</strong> mortality and to evaluate the impact <strong>of</strong> methicillin<br />
resistance on outcome in patients with . Staphylococcus aureus infection according to underlying conditions and type <strong>of</strong><br />
infection. Methods: An observational cohort study including 4949 patients with . S. aureus infection was conducted. We<br />
compared data from patients with MRSA infection with those with MSSA infection. Results: The 30-day mortality rate <strong>of</strong> MRSA<br />
group was significantly higher than that <strong>of</strong> MSSA group (15.6% vs. 6.2%, . P < 0.001). However, MRSA infection was not found<br />
to be independent risk factor for mortality after adjusting for other variables (OR = 1.03, 95% CI = 0.80-1.32). When we<br />
analyzed patients with . S. aureus bacteremia (n = 709), MRSA infection was found to be significantly associated with mortality<br />
in multivariate analysis (Adjusted OR = 1.69, 95% CI = 1.15-2.49). When the 30-day mortality rates were compared according<br />
to underlying diseases, the 30-day mortality rate <strong>of</strong> MRSA group was significantly higher than that <strong>of</strong> MSSA group in patients<br />
with malignancy or renal diseases. MRSA infection was also found to be one <strong>of</strong> the independent risk factors for mortality in<br />
patients with malignancy (adjusted OR = 1.69, 95% CI = 1.06-2.70) and in those with renal disease (adjusted OR = 1.70, 95% CI<br />
181
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
= 1.0-2.89), after adjustment for host variables. Conclusions: Methicillin resistance adversely affected the outcome <strong>of</strong> patients<br />
with . S. aureus infection, in patients with cancer or renal disease and in those with . S. aureus bacteremia, although MRSA<br />
infection was not found to be significantly associated with higher mortality in overall patient population. © <strong>2010</strong> The British<br />
Infection Society.<br />
No.493<br />
Author (s) : Kangwanariyakul, Y., Nantasenamat, C., Tantimongcolwat, T., Naenna, T.<br />
Title : Data mining <strong>of</strong> magnetocardiograms for prediction <strong>of</strong> ischemic heart disease<br />
Source : EXCLI Journal, Volume 9, <strong>2010</strong>, Pages 82-95<br />
Document Type : Article<br />
Keywords : Back-propagation neural network; Bayesian Neural network; Data mining; Ischemia; Magnetocardiography; Probabilistic<br />
Neural network; Support vector machine<br />
<strong>Abstract</strong> : Ischemic Heart Disease (IHD) is a major cause <strong>of</strong> death. Early and accurate detection <strong>of</strong> IHD along with rapid diagnosis are<br />
important for reducing the mortality rate. Magnetocardiogram (MCG) is a tool for detecting electro-physiological activity <strong>of</strong> the<br />
myocardium. MCG is a fully non-contact method, which avoids the problems <strong>of</strong> skin-electrode contact in the<br />
Electrocardiogram (ECG) method. However, the interpretation <strong>of</strong> MCG recordings is time-consuming and requires analysis by<br />
an expert. Therefore, we propose the use <strong>of</strong> machine learning for identification <strong>of</strong> IHD patients. Back-propagation neural<br />
network (BPNN), the Bayesian neural network (BNN), the probabilistic neural network (PNN) and the support vector machine<br />
(SVM) were applied to develop classification models for identifying IHD patients. MCG data was acquired by sequential<br />
measurement, above the torso, <strong>of</strong> the magnetic field emitted by the myocardium using a J-T interval <strong>of</strong> 125 cases. The<br />
training and validation data <strong>of</strong> 74 cases employed 10-fold cross-validation methods to optimize support vector machine and<br />
neural network parameters. The predictive performance was assessed on the testing data <strong>of</strong> 51 cases using the following<br />
metrics: accuracy, sensitivity, and specificity and area under the receiver operating characteristic (ROC) curve. The results<br />
demonstrated that both BPNN and BNN displayed the highest and the same level <strong>of</strong> accuracy at 78.43%. Furthermore, the<br />
decision threshold and the area under the ROC curve was -0.2774 and 0.9059, respectively, for BPNN and 0.0470 and 0.8495,<br />
respectively, for BNN. This indicated that BPNN was the best classification model, BNN was the best performing model with<br />
sensitivity <strong>of</strong> 96.65%, and SVM employing the radial basis function kernel displayed the highest specificity <strong>of</strong> 86.36%.<br />
No.494<br />
Author (s) : Kanitvittaya, S., Suksai, U., Suksripanich, O., Pobkeeree, V.<br />
Title : Laboratory quality improvement in Thailand's northernmost provinces<br />
Source : <strong>International</strong> Journal <strong>of</strong> Health Care Quality Assurance, Volume 23, Issue 1, 1 January <strong>2010</strong>, Pages 22-34<br />
Document Type : Article<br />
Keywords : Assessment; Laboratories; Quality; Standards; Thailand<br />
<strong>Abstract</strong> : Purpose: In Thailand nearly 1,000 public health laboratories serve 65 million people. A qualified indicator <strong>of</strong> a good quality<br />
laboratory is Thailand Medical Technology Council certification. Consequently, Chiang Rai Regional Medical Sciences Center<br />
established a development program for laboratory certification for 29 laboratories in the province. This paper seeks to<br />
examine this issue. Design/methodology/approach: The goal was to improve laboratory service quality by voluntary<br />
participation, peer review, training and compliance with standards. The program consisted <strong>of</strong> specific activities. Training and<br />
workshops to update laboratory staffs' quality management knowledge were organized. Staff in each laboratory performed a<br />
self-assessment using a standard check-list to evaluate ten laboratory management areas. Chiang Rai Regional Medical<br />
Sciences Center staff supported the distribution <strong>of</strong> quality materials and documents. They provided calibration services for<br />
laboratory equipment. Peer groups performed an internal audit and successful laboratories received Thailand Medical<br />
Technology Council certification. Findings: By December 2007, eight <strong>of</strong> the 29 laboratories had improved quality sufficiently to<br />
be certified. Factors that influenced laboratories' readiness for quality improvement included the number <strong>of</strong> staff, their<br />
knowledge, budget and staff commitment to the process. Moreover, the support <strong>of</strong> each hospital's laboratory working group<br />
or network was essential for success. Research limitations/implications: There was no clear policy for supporting the program.<br />
Laboratories voluntarily conducted quality management using existing resources. Practical implications: A bottom-up<br />
approach to this kind <strong>of</strong> project can be difficult to accomplish. Laboratory pr<strong>of</strong>essionals can work together to illustrate and<br />
highlight outcomes for top-level health <strong>of</strong>ficials. A top-down, practical approach would be much less difficult to implement.<br />
Originality/value: Quality certification is a critical step for laboratory staff, which also encourages them to aspire to<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
international quality standards like ISO. The certification program is an important mechanism for addressing inadequate<br />
knowledge, budget, planning, policy and staff required to improve laboratory services. © Emerald Group Publishing Limited.<br />
No.495<br />
Author (s) : Kanjun, K.; Uawithya, P.<br />
Title : EXPRESSION OF RECOMBINANT AQUAPORIN 4 PROTEIN FOR DETECTION OF ANTI-AQUAPORIN 4 IN NEUROMYELITIS OPTICA<br />
DISEASE<br />
Source : JOURNAL OF NEUROCHEMISTRY, Volume: 115, Supplement: 1, Pages: 73-73, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.496<br />
Author (s) : Kankaew, P., Leemingsawat, S., Apiwathnasorn, C., Thongrungkiat, S., Ponlawat, A.<br />
Title : DISTRIBUTION AND IDENTIFICATION OF ANOPHELES BARBIROSTRIS/CAMPESTRIS AT SA KAEO PROVINCE IN THAILAND<br />
Source : AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, Volume: 83, Issue: 5, Supplement: S, Pages: 61-61, Meeting<br />
<strong>Abstract</strong>: 203, Published: NOV <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.497<br />
Author (s) : Kanlaya, R., Pattanakitsakul, S.-N., Sinchaikul, S., Chen, S.-T., Thongboonkerd, V.<br />
Title : The ubiquitin-proteasome pathway is important for dengue virus infection in primary human endothelial cells<br />
Source : Journal <strong>of</strong> Proteome Research, Volume 9, Issue 10, 1 October <strong>2010</strong>, Pages 4960-4971<br />
Document Type : Article<br />
Keywords : Dengue; proteasome; proteomics; therapeutic targets; ubiquitin<br />
<strong>Abstract</strong> : Dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) are the most severe forms <strong>of</strong> dengue virus infection with<br />
hemorrhage and plasma leakage. However, pathogenic mechanisms <strong>of</strong> DHF and DSS remain poorly understood. We therefore<br />
investigated host responses as determined by changes in the cellular proteome <strong>of</strong> primary human endothelial cells upon<br />
infection with dengue virus serotype 2 (DEN-2) at a multiplicity <strong>of</strong> infection (MOI) <strong>of</strong> 10 for 24 h. Two-dimensional PAGE and<br />
quantitative intensity analysis revealed 38 significantly altered protein spots (16 upregulated and 22 downregulated) in DEN-2infected<br />
cells compared to mock controls. These altered proteins were successfully identified by mass spectrometry,<br />
including those involved in oxidative stress response, transcription and translation, cytoskeleton assembly, protein<br />
degradation, cell growth regulation, apoptosis, cellular metabolism, and antiviral response. The proteomic data were<br />
validated by Western blot analyses [upregulated ubiquitin-activating enzyme E1 (UBE1) and downregulated annexin A2] and<br />
an immun<strong>of</strong>luorescence study (upregulated MxA). Interestingly, we found that MxA was colocalized with DEN-2 viral capsid<br />
protein, strengthening its role as an antiviral protein. Moreover, we also identified upregulation <strong>of</strong> a proteasome subunit. Our<br />
functional study revealed the significant role <strong>of</strong> ubiquitination in dengue infection and UBE1 inhibition by its specific inhibitor<br />
(UBEI-41) caused a significant reduction in the level <strong>of</strong> viral protein synthesis and its infectivity. Our findings suggest that<br />
various biological processes were triggered in response to dengue infection, particularly antiviral IFN and ubiquitin-proteasome<br />
pathways. © <strong>2010</strong> American Chemical Society.<br />
No.498<br />
Author (s) : Kanlaya, R., Pattanakitsakul, S.-N., Sinchaikul, S., Chen, S.-T., Thongboonkerd, V.<br />
Title : Vimentin interacts with heterogeneous nuclear ribonucleoproteins and dengue nonstructural protein 1 and is important for<br />
viral replication and release<br />
Source : Molecular BioSystems, Volume 6, Issue 5, <strong>2010</strong>, Pages 795-806<br />
Document Type : Article<br />
Keywords : heterogeneous nuclear ribonucleoprotein, vimentin, virus protein; article, cell line, Dengue<br />
virus, human, immunoprecipitation, metabolism, protein binding, tandem mass spectrometry, two dimensional gel<br />
electrophoresis; Cell Line, Dengue Virus, Electrophoresis, Gel, Two-Dimensional, Heterogeneous-Nuclear<br />
183
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Ribonucleoproteins, Humans, Immunoprecipitation, Protein Binding, Tandem Mass Spectrometry, Vimentin, Viral Nonstructural<br />
Proteins; Dengue virus<br />
<strong>Abstract</strong> : Our previous study using expression proteomics demonstrated that many proteins, particularly five forms <strong>of</strong> heterogeneous<br />
nuclear ribonucleoproteins (hnRNPs), were up-regulated in human endothelial cells upon dengue virus infection. To address<br />
functional significance <strong>of</strong> these proteins in response to dengue virus infection, we performed a functional proteomics study to<br />
identify hnRNPs-interacting proteins in the infected EA.hy926 cells. Immunoprecipitation followed by 2-D PAGE and mass<br />
spectrometric analyses revealed 18 and 13 interacting partners <strong>of</strong> hnRNP C1/C2 and hnRNP K, respectively. Interestingly,<br />
vimentin was a common partner for both hnRNP C1/C2 and K. The interaction between vimentin and these hnRNPs was<br />
confirmed by reciprocal immunoprecipitation followed by Western blot analysis and also by double immun<strong>of</strong>luorescence<br />
staining. Disruption <strong>of</strong> vimentin intermediate filament by acrylamide not only dissociated these complexes but also reduced<br />
nuclear hnRNPs expression, whereas cytosolic hnRNPs expression was unchanged. We also demonstrated that vimentin was<br />
strongly associated with dengue non-structural protein 1 (NS1). Disruption <strong>of</strong> vimentin intermediate filament not only<br />
dissociated this complex but also reduced dengue NS1 expression, as well as viral replication and release. Our data report for<br />
the first time that vimentin interacts with hnRNPs and dengue NS1, and plays a crucial role in replication and release <strong>of</strong><br />
dengue virus. © <strong>2010</strong> The Royal Society <strong>of</strong> Chemistry.<br />
No.499<br />
Author (s) : Kanlayanaphotporn, R., Chiradejnant, A., Vachalathiti, R.<br />
Title : Immediate effects <strong>of</strong> the central posteroanterior mobilization technique on pain and range <strong>of</strong> motion in patients with<br />
mechanical neck pain<br />
Source : Disability and Rehabilitation, Volume 32, Issue 8, <strong>2010</strong>, Pages 622-628<br />
Document Type : Article<br />
Keywords : Manual therapy; Mobilization; Neck pain<br />
<strong>Abstract</strong> : Purpose. To determine the immediate effects <strong>of</strong> the central posteroanterior (PA) mobilization technique on both pain and<br />
active cervical range <strong>of</strong> motion in patients with mechanical neck pain presenting with central or bilateral symptoms. Methods.<br />
A randomized controlled trial was conducted in 60 patients who were randomly allocated into either 'central PA' or 'random'<br />
mobilization group. Two physical therapists and one assessor participated. Outcome measures included neck pain at rest,<br />
pain on the most painful movement, and active cervical range <strong>of</strong> motion taken before and immediately 5min after the<br />
mobilization treatment. Results. Significant reductions in pain at rest and on the most painful movement were noted withingroup<br />
comparisons (p
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.501<br />
Author (s) : Kantabutra, S.<br />
Title : Vision effects: A critical gap in educational leadership research<br />
Source : <strong>International</strong> Journal <strong>of</strong> Educational Management, Volume 24, Issue 5, <strong>2010</strong>, Pages 376-390<br />
Document Type : Article<br />
Keywords : Educational planning and administration; Higher education; Leadership; Performance management<br />
<strong>Abstract</strong> : Purpose: Although leaders are widely believed to employ visions, little is known about what constitutes an "effective" vision,<br />
particularly in the higher education sector. This paper seeks to proposes a research model for examining relationships<br />
between vision components and performance <strong>of</strong> higher education institutions, as measured by financial stability, student<br />
satisfaction and growth, process improvement, and learning and faculty satisfaction. The model proposes that vision<br />
attributes <strong>of</strong> brevity, clarity, abstractness, challenge, future orientation, stability, and desirability, and vision content relating to<br />
financial stability, student satisfaction and growth, process improvement, and learning and faculty satisfaction can affect<br />
performance through four vision realisation variables. Design/methodology/approach: Based on a critical review <strong>of</strong> existing<br />
theoretical concepts and empirical evidence, a new research model, as well as research hypotheses, are developed for future<br />
research. Findings: With future empirical support, the model will help university and college administrators to effectively<br />
improve their institutional performance. Originality/value: While vision is core to the prevailing vision-based leadership<br />
theories, little is theoretically and empirically known about attributes for effective visions, particularly in the education sector.<br />
The paper proposes a model for future research to fill this gap. © Emerald Group Publishing Limited.<br />
No.502<br />
Author (s) : Kantabutra, S., Avery, G.C.<br />
Title : The power <strong>of</strong> vision: Statements that resonate<br />
Source : Journal <strong>of</strong> Business Strategy, Volume 31, Issue 1, 1 January <strong>2010</strong>, Pages 37-45<br />
Document Type : Article<br />
Keywords : Leadership; Mission statements; Transformational leadership<br />
<strong>Abstract</strong> : Purpose: The purpose <strong>of</strong> the paper is to identify characteristics <strong>of</strong> visions that are associated with desirable performance<br />
outcomes. Design/methodology/approach: The paper examines findings from various vision studies in American, Australian<br />
and Thai businesses to derive characteristics <strong>of</strong> effective vision statements. Findings: Effective visions are characterized by<br />
conciseness, clarity, abstractness, stability, future orientation, challenge and desirability or ability to inspire in Australian, Thai<br />
and US businesses. Such visions are associated with higher performance outcomes.Originality/valueWhile vision is emphasized<br />
by authors and consultants as critical to superior performance, little is known about what characterizes effective vision. This<br />
study uncovers this unknown. © Emerald Group Publishing Limited.<br />
No.503<br />
Author (s) : Kantabutra, S., Vimolratana, P.<br />
Title : Vision-based leaders and their followers in retail stores: Relationships and consequences in Australia<br />
Source : Journal <strong>of</strong> Applied Business Research, Volume 26, Issue 6, November <strong>2010</strong>, Pages 123-134<br />
Document Type : Article<br />
Keywords : Emotions; Followership; Leadership; Motivation; Passion; Vision<br />
<strong>Abstract</strong> : Leaders are exhorted to espouse vision, but little is known about how vision is used by leaders in Australian retail stores. The<br />
present study tested relationships between store manager passion for vision, motivation <strong>of</strong> staff and use <strong>of</strong> vision among<br />
staff, and leadership outcomes <strong>of</strong> staff and customer satisfaction in Australian apparel retail stores. Stores with vision are<br />
associated with higher staff satisfaction and more frequent use <strong>of</strong> vision among staff. Store manager passion directly predicts<br />
improvements in motivation <strong>of</strong> staff, use <strong>of</strong> vision among staff and staff satisfaction. Motivation <strong>of</strong> staff directly predicts<br />
enhanced staff satisfaction, while use <strong>of</strong> vision among staff directly predicts improved staff and customer satisfaction.<br />
No.504<br />
Author (s) : Kanthong, N., Khemnu, N., Pattanakitsakul, S.-N., Malasit, P., Flegel, T.W.<br />
Title : Persistent, triple-virus co-infections in mosquito cells<br />
Source : BMC Microbiology, Volume 10, <strong>2010</strong>, Article number 14<br />
Document Type : Article<br />
185
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : antigen detection, article, cellular distribution, confocal microscopy, Dengue virus, Densovirus, immunocytochemistry, insect<br />
cell culture, Japanese encephalitis virus, mosquito, nonhuman, persistent virus infection, superinfection, animal, cell<br />
line, cytology, disease carrier, flow cytometry, pathogenicity, virology, virus infection; Arbovirus, Crustacea, Dengue<br />
virus, Densovirus, Hexapoda, Japanese encephalitis virus, Parvovirus; Animals, Cell Line, Culicidae, Dengue<br />
Virus, Densovirus, Encephalitis Virus, Japanese, Flow Cytometry, Insect Vectors, Microscopy, Confocal, Virus Diseases<br />
<strong>Abstract</strong> : Background. It is known that insects and crustaceans can carry simultaneous, active infections <strong>of</strong> two or more viruses without<br />
showing signs <strong>of</strong> disease, but it was not clear whether co-infecting viruses occupied the same cells or different cells in<br />
common target tissues. Our previous work showed that successive challenge <strong>of</strong> mosquito cell cultures followed by serial,<br />
split-passage resulted in stabilized cultures with 100% <strong>of</strong> the cells co-infected with Dengue virus (DEN) and an insect<br />
parvovirus (densovirus) (DNV). By addition <strong>of</strong> Japanese encephalitis virus (JE), we tested our hypothesis that stable, persistent,<br />
triple-virus co-infections could be obtained by the same process. Results. Using immunocytochemistry by confocal<br />
microscopy, we found that JE super-challenge <strong>of</strong> cells dually infected with DEN and DNV resulted in stable cultures without<br />
signs <strong>of</strong> cytopathology, and with 99% <strong>of</strong> the cells producing antigens <strong>of</strong> the 3 viruses. Location <strong>of</strong> antigens for all 3 viruses in<br />
the triple co-infections was dominant in the cell nuclei. Except for DNV, this differed from the distribution in cells persistently<br />
infected with the individual viruses or co-infected with DNV and DEN. The dependence <strong>of</strong> viral antigen distribution on single<br />
infection or co-infection status suggested that host cells underwent an adaptive process to accommodate 2 or more viruses.<br />
Conclusions. Individual mosquito cells can accommodate at least 3 viruses simultaneously in an adaptive manner. The<br />
phenomenon provides an opportunity for genetic exchange between diverse viruses and it may have important medical and<br />
veterinary implications for arboviruses. © <strong>2010</strong> Kanthong et al; licensee BioMed Central Ltd.<br />
No.505<br />
Author (s) : Kanthong, N., Laosutthipong, C., Flegel, T.W.<br />
Title : Response to Dengue virus infections altered by cytokine-like substances from mosquito cell cultures<br />
Source : BMC Microbiology, Volume 10, <strong>2010</strong>, Article number 290<br />
Document Type : Article<br />
Keywords : proteinase K, cytokine; apoptosis, article, cell activity, cell culture, cell separation, confocal microscopy, controlled<br />
study, dengue, Dengue virus 2, immun<strong>of</strong>luorescence, mosquito, nonhuman, shrimp, supernatant, treatment outcome, virus<br />
cell interaction, Aedes, animal, cell line, Dengue virus, disease carrier, immunology, physiology, virology; Apis<br />
mellifera, Crustacea, Decapoda (Crustacea), Dengue virus, Hexapoda; Aedes, Animals, Cell Line, Cytokines, Dengue Virus, Insect<br />
Vectors<br />
<strong>Abstract</strong> : Background. With both shrimp and commercial insects such as honey bees, it is known that stable, persistent viral infections<br />
characterized by absence <strong>of</strong> disease can sometimes shift to overt disease states as a result <strong>of</strong> various stress triggers and that<br />
this can result in serious economic losses. The main research interest <strong>of</strong> our group is to understand the dynamics <strong>of</strong> stable<br />
viral infections in shrimp and how they can be destabilized by stress. Since there are no continuous cell lines for crustaceans,<br />
we have used a C6/36 mosquito cell line infected with Dengue virus to test hypotheses regarding these interactions. As a<br />
result, we accidentally discovered two new cytokine-like substances in 5 kDa extracts from supernatant solutions <strong>of</strong> acutely<br />
and persistently infected mosquito cells. Results. Nave C6/36 cells were exposed for 48 h to 5 kDa membrane filtrates<br />
prepared from the supernatant medium <strong>of</strong> stable C6/36 mosquito cell cultures persistently-infected with Dengue virus.<br />
Subsequent challenge <strong>of</strong> nave cells with a virulent stock <strong>of</strong> Dengue virus 2 (DEN-2) and analysis by confocal<br />
immun<strong>of</strong>luorescence microscopy using anti-DEN-2 antibody revealed a dramatic reduction in the percentage <strong>of</strong> DEN-2<br />
infected cells when compared to control cells. Similar filtrates prepared from C6/36 cells with acute DEN-2 infections were<br />
used to treat stable C6/36 mosquito cell cultures persistently-infected with Dengue virus. Confocal immun<strong>of</strong>luorescence<br />
microscopy revealed destabilization in the form <strong>of</strong> an apoptosis-like response. Proteinase K treatment removed the cellaltering<br />
activities indicating that they were caused by small polypeptides similar to those previously reported from insects.<br />
Conclusions. This is the first report <strong>of</strong> cytokine-like substances that can alter the responses <strong>of</strong> mosquito cells to Dengue virus.<br />
This simple model system allows detailed molecular studies on insect cytokine production and on cytokine activity in a<br />
standard insect cell line. © <strong>2010</strong> Kanthong et al; licensee BioMed Central Ltd.<br />
No.506<br />
Author (s) : Kaojarern, S.; Masaya-anon, N.; Pongcharoensuk, P.; Pattanaprateep, O.<br />
Title : FACTORS INFLUENCED ORAL OXICAM AND COXIBS' EXPENDITURE AT A THAI TEACHING HOSPITAL, FISCAL YEAR 2006-2009<br />
Source : VALUE IN HEALTH, Volume: 13, Issue: 7, Pages: A559-A560, Published: NOV <strong>2010</strong><br />
186
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.507<br />
Author (s) : Kaojarern, S.; Ongphiphadhanakul, B.; Pattanaprateep, O.<br />
Title : PATTERN OF LIPID MODIFYING AGENTS PRESCRIPTION AMONG CLUSTERS OF PHYSICIAN IN A THAI TEACHING HOSPITAL, FISCAL<br />
YEAR 2009<br />
Source : VALUE IN HEALTH, Volume: 13, Issue: 7, Pages: A521-A521, Published: NOV <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.508<br />
Author (s) : Karema, C., Imwong, M., Fanello, C.I., Stepniewska, K., Uwimana, A., Nakeesathit, S., Dondorp, A., Day, N.P., White, N.J.<br />
Title : Molecular correlates <strong>of</strong> high-level antifolate resistance in Rwandan children with Plasmodium falciparum malaria<br />
Source : Antimicrobial Agents and Chemotherapy, Volume 54, Issue 1, January <strong>2010</strong>, Pages 477-483<br />
Document Type : Article<br />
Keywords : amodiaquine, amodiaquine plus sulfadoxine pyrimethamine, artesunate plus chlorproguanil plus<br />
dapsone, pyrimethamine, sulfadoxine, unclassified drug; Africa, antimalarial drug resistance, article, child, clinical<br />
trial, controlled clinical trial, controlled study, DNA polymorphism, drug efficacy, drug sensitivity, gene, gene frequency, gene<br />
mutation, haplotype, human, infant, major clinical study, malaria falciparum, multidrug resistance, pfdhfr gene, pfdhps<br />
gene, Plasmodium falciparum, preschool child, prevalence, priority journal, randomized controlled trial, Rwanda, treatment<br />
failure; Analysis <strong>of</strong> Variance, Antimalarials, Child, Child, Preschool, Drug Combinations, Drug Resistance, Folic Acid<br />
Antagonists, Gene Frequency, Genes, Protozoan, Haplotypes, Humans, Infant, Malaria, Falciparum, Mutation, Plasmodium<br />
falciparum, Polymorphism, Genetic, Pyrimethamine, Rwanda, Sulfadoxine, Tetrahydr<strong>of</strong>olate Dehydrogenase<br />
<strong>Abstract</strong> : Antifolate drugs have an important role in the treatment <strong>of</strong> malaria. Polymorphisms in the genes encoding the dihydr<strong>of</strong>olate<br />
reductase and dihydropteroate synthetase enzymes cause resistance to the antifol and sulfa drugs, respectively. Rwanda has<br />
the highest levels <strong>of</strong> antimalarial drug resistance in Africa. We correlated the efficacy <strong>of</strong> chlorproguanil-dapsone plus<br />
artesunate (CPG-DDS+A) and amodiaquine plus sulfadoxinepyrimethamine (AQ+SP) in children with uncomplicated malaria<br />
caused by Plasmodium falciparum parasites with pfdhfr and pfdhps mutations, which are known to confer reduced drug<br />
susceptibility, in two areas <strong>of</strong> Rwanda. In the eastern province, where the cure rates were low, over 75% <strong>of</strong> isolates had three<br />
or more pfdhfr mutations and two or three pfdhps mutations and 11% had the pfdhfr 164-Leu polymorphism. In the western<br />
province, where the cure rates were significantly higher (P < 0.001), the prevalence <strong>of</strong> multiple resistance mutations was<br />
lower and the pfdhfr I164L polymorphism was not found. The risk <strong>of</strong> treatment failure following the administration <strong>of</strong> AQ+SP<br />
more than doubled for each additional pfdhfr resistance mutation (odds ratio [OR] = 2.4; 95% confidence interval [CI] = 1.01<br />
to 5.55; P = 0.048) and each pfdhps mutation (OR = 2.1; 95% CI = 1.21 to 3.54; P = 0.008). The risk <strong>of</strong> failure following CPG-<br />
DDS+A treatment was 2.2 times higher (95% CI = 1.34 to 3.7) for each additional pfdhfr mutation, whereas there was no<br />
association with mutations in the pfdhps gene (P = 0.13). The pfdhfr 164-Leu polymorphism is prevalent in eastern Rwanda.<br />
Antimalarial treatments with currently available antifol-sulfa combinations are no longer effective in Rwanda because <strong>of</strong> highlevel<br />
resistance. Copyright © <strong>2010</strong>, American Society for Microbiology. All Rights Reserved.<br />
No.509<br />
Author (s) : Karoopongse, E., Khuhapinant, A., Auewarakul, C.U.<br />
Title : A rare occurrence <strong>of</strong> hairy cell leukemia in the Thai population: a case report.<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand = Chotmaihet thangphaet, Volume 93 Suppl 1, January <strong>2010</strong>, Pages S196-202<br />
Document Type : Article<br />
Keywords : antineoplastic agent, cyclophosphamide, drug derivative, fludarabine, vidarabine; adult, article, Asian, bone marrow, case<br />
report, flow cytometry, hairy cell leukemia, human, immunophenotyping, male, pathology, splenectomy, treatment outcome;<br />
Adult, Antineoplastic Agents, Asian Continental Ancestry Group, Bone Marrow, Cyclophosphamide, Flow<br />
Cytometry, Humans, Immunophenotyping, Leukemia, Hairy Cell, Male, Splenectomy, Treatment Outcome, Vidarabine<br />
187
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : Hairy cell leukemia (HCL) has been mainly reported from the Western countries. Herein we describe a case <strong>of</strong> HCL diagnosed<br />
in a Thai patient. A 36-year-old man presented with abdominal discomfort, frequent gum bleeding and significant weight loss<br />
for 2 months. Physical examination revealed moderate anemia, petechial hemorrhage on the extremities and an enlarged<br />
spleen down to the umbilicus. No hepatomegaly or lymphadenopathy was detected. Complete blood counts revealed a<br />
hemoglobin (Hb) <strong>of</strong> 6.6 g/dL, a white blood cell (WBC) count <strong>of</strong> 1.6 x 10(9)/L (neutrophil 16%, lymphocyte 71%, monocyte<br />
11%, atypical lymphocyte 1%), and a platelet (PLT) count <strong>of</strong> 17 x 10(9)/L. Abnormal large mononuclear cells with villous<br />
projections were seen in the blood smear. Although bone marrow (BM) aspiration resulted in a dry tap, abnormal<br />
lymphocytes with villous projections could again be identified in the touch preparation. Flow cytometric analysis showed a<br />
distinct population above the normal lymphocyte region on CD45/SSC gates with a strong expression <strong>of</strong> CD19, CD20, CD22,<br />
CD25, CD11c, and kappa. CD5, CD23, CD10, CD4, and CD8 were all negative. BM biopsy was consistent with HCL. The patient<br />
was treated with splenectomy followed by 8 cycles <strong>of</strong> fludarabine and cyclophosphamide chemotherapy. At 21 months after<br />
diagnosis, the patient was doing well with a Hb <strong>of</strong> 16.9 g/dl, a WBC count <strong>of</strong> 6.8 x 10(9)/L, neutrophil 49.9%, lymphocyte<br />
39.6%, monocyte 8.6%, and a PLT count <strong>of</strong> 329 x 10(9)/L). No abnormal lymphoid cells were detected in the blood smear.<br />
This present report represents the first Thai HCL case that was immunophenotypically confirmed by flow cytometry and<br />
successfully treated at Siriraj Hospital.<br />
No.510<br />
Author (s) : Karunaweera, N.D.; Dewasurendra, R., Fernando, D., Sereejaitham, P., Suriyaphol, P.<br />
Title : GENETIC MARKERS AND RISK OF MALARIA INFECTIONS: GENETIC-EPIDEMIOLOGY STUDY IN A LOW MALARIA ENDEMIC AREA OF<br />
SRI LANKA<br />
Source : AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, Volume: 83, Issue: 5, Supplement: S, Pages: 219-220, Meeting<br />
<strong>Abstract</strong>: 738, Published: NOV <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.511<br />
Author (s) : Kasemsuwan, L., Cheewaruangroj, W., Tungkeeratichai, J., Bhongmakapat, T., Thawin, C., Lertsukprasert, K., Tiravanitchakul, R.,<br />
Dara, R., Laothamatas, J.<br />
Title : Fourteen-years experience with cochlear implantation in ramathibodi hospital<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 12, December <strong>2010</strong>, Pages 1399-1405<br />
Document Type : Review<br />
Keywords : Cochlear implant; Cochleostomy; Ct scans <strong>of</strong> temporal bone; Hearing loss<br />
<strong>Abstract</strong> : Objective: To review the cochlear implant program in Ramathibodi Hospital and share experience <strong>of</strong> cochlear implantation<br />
emphasized on clinical and surgical outcomes. Material and Method: Retrospective review <strong>of</strong> 143 ears (140 patients) operated<br />
with cochlear implant between 1995 and 2009. The demographic data including etiology <strong>of</strong> deafness and findings from<br />
temporal bone CT scans were reviewed. The authors' experience with cochlear implant surgery in terms <strong>of</strong> patient selection,<br />
patient advisory clinic, necessary equipment, pre- and postoperative evaluations, surgical techniques and complications were<br />
discussed. Results: Most congenital origin was unknown etiology and congenital rubella was the most common known cause.<br />
From the CT scans <strong>of</strong> congenital deafness, vestibular aqueduct dilatation was the most common and found in 29.31% while<br />
Mondini malformation was shown to be 16.37%. The authors' surgical technique <strong>of</strong> using the pocket method and designed<br />
bony ridge at cortical mastoid rim had helped stabilizing the implant and electrode fancoil. During the last two years, no<br />
complication or revision surgery was detected. Conclusion: Cochlear implant surgery in both children and adults can result in<br />
good surgical outcome and fewer complications under experienced surgeons and a good team.<br />
No.512<br />
Author (s) : Kaset, C., Eursitthichai, V., Vichasri-Grams, S., Viyanant, V., Grams, R.<br />
Title : Rapid identification <strong>of</strong> lymnaeid snails and their infection with Fasciola gigantica in Thailand<br />
Source : Experimental Parasitology, Volume 126, Issue 4, December <strong>2010</strong>, Pages 482-488<br />
Document Type : Article<br />
Keywords : 16S ribosomal DNA; Austropeplea viridis; Cathepsin L; Fasciola gigantica; Lymnaeidae; Multiplex PCR; Radix<br />
rubiginosa; Radix swinhoei; Thailand; Trematoda<br />
188
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : Freshwater snails <strong>of</strong> the family Lymnaeidae are the intermediate hosts <strong>of</strong> the liver fluke Fasciola worldwide. While distinct<br />
species have been identified at the molecular level in other parts <strong>of</strong> the world such data have not been published for<br />
Thailand. In this study we collected Lymnaeidae from different localities across Thailand and analyzed their 16S rDNA<br />
sequences as a molecular signature for classification. In addition to the ubiquitous Radix rubiginosa, we have confirmed the<br />
presence <strong>of</strong> Austropeplea viridis and Radix swinhoei, for the latter <strong>of</strong> which the ribosomal rDNA sequences are reported for<br />
the first time, in North-Thailand. Based on the obtained 16S rDNA data three primer pairs were designed that allowed rapid<br />
identification <strong>of</strong> these snail species by PCR. To determine their infection status, PCR primers for F. gigantica cathepsin L were<br />
used in parallel with the snail 16S rDNA species-specific primers in multiplex PCR analyses. Western blot analysis <strong>of</strong> total snail<br />
protein with a monoclonal anti-F. gigantica cathepsin L antibody confirmed positive cathepsin L PCR results. The developed<br />
diagnostic PCR will be <strong>of</strong> use in risk assessment for transmission <strong>of</strong> fascioliasis in Thailand. © Elsevier Inc.<br />
No.513<br />
Author (s) : Kasetrtut, C., Kaeoket, K.<br />
Title : Effect <strong>of</strong> using supernatant for post-thawing solution and semen extender prior to insemination on sow reproductive<br />
performance<br />
Source : Thai Journal <strong>of</strong> Veterinary Medicine, Volume 40, Issue 2, <strong>2010</strong>, Pages 171-178<br />
Document Type : Article<br />
Keywords : Artificial insemination; Boar semen; Cryopreservation; Supernatant<br />
<strong>Abstract</strong> : The objective <strong>of</strong> the present study was to determine the effects <strong>of</strong> using supernatant (seminal plasma: extender, 50:50 v/v) as<br />
post-thawing solution and semen extender on sow fertility. Fifteen sows were allocated into the following experimental<br />
groups: Groups A (control), sows (n=5) were inseminated with fresh semen, using a dose <strong>of</strong> 4×109 spermatozoa in 60 ml <strong>of</strong><br />
Modena extender; Group B, sows (n=5) were inseminated with frozen semen (supplemented with 10 mM <strong>of</strong> L-cysteine),<br />
using a dose <strong>of</strong> 2×109 spermatozoa in 60 ml <strong>of</strong> supernatant (50% v/v <strong>of</strong> seminal plasma plus ModenaTM extender); Group C,<br />
sows (n=5) were inseminated with frozen semen (supplemented with 10 mM <strong>of</strong> L-cysteine), using a dose <strong>of</strong> 2×109<br />
spermatozoa in 60 ml <strong>of</strong> Modena extender. All sows were inseminated twice using an intrauterine catheter depending on<br />
their weaning to oestrous interval (WOI). Pregnancy rate (PR), farrowing rate (FR), total number <strong>of</strong> piglet born (TNB) and<br />
number <strong>of</strong> piglet born alive (NBA) were recorded. In group A, the PR and FR were 100%, TNB and NBA were 7.8±3.9 and<br />
7±3.9, respectively. For frozen semen, PR, FR, TNB and NBA in group B were higher than group C (100% versus 60%, 60%<br />
versus 0%, 6.0±2.7 versus 0, 6.0±2.7 versus 0, respectively). In conclusion, using supernatant (50% v/v <strong>of</strong> seminal plasma plus<br />
ModenaTM) as post-thawing solution and semen extender for artificial insemination in field condition improve sow fertility.<br />
No.514<br />
Author (s) : Kasi, A.K., Kasi, J.K., Afzulpurkar, N., Bohez, E., Tuantranont, A., Mahaisavariya, B.<br />
Title : Fabrication <strong>of</strong> anodic aluminum oxide (AAO) nano-porous membrane on both sides <strong>of</strong> aluminum sheet<br />
Source : ICMEE <strong>2010</strong> - <strong>2010</strong> 2nd <strong>International</strong> Conference on Mechanical and Electronics Engineering, Proceedings, Volume 2, <strong>2010</strong>,<br />
Article number 5558475, Pages V2122-V2126<br />
Document Type : Conference Paper<br />
Keywords : AAO membrane, Anodic aluminum oxide, Anodizations, Commercial grade, Electropolishing, Fabrication process, Hard<br />
anodization, Nanoporous membrane, Pure aluminum, Speed-ups, Total costs; Aluminum, Aluminum sheet, Electronics<br />
engineering, Membranes, Paper sheeting; Fabrication<br />
<strong>Abstract</strong> : Fabrication <strong>of</strong> AAO Membrane is a time consuming and sensitive process. Several attempts have made to speed up this<br />
process. This paper reports the fabrication <strong>of</strong> AAO membrane at both sides <strong>of</strong> aluminum sheet. This method speeds up the<br />
fabrication process. This method <strong>of</strong> fabrication is easier because the aluminum sheet does not need to protect from the back<br />
side. This type <strong>of</strong> fabrication minimizes the total cost <strong>of</strong> nano-porous membrane. The fabrication <strong>of</strong> nano-porous membrane<br />
has been done by homemade system. The developed system can be used for mild anodization (MA), hard anodization (HA)<br />
and electro-polishing. This paper represents the fabrication <strong>of</strong> AAO membrane using 99.999% pure aluminum and commercial<br />
grade impure aluminum. The fabrication was done by MA and HA. © <strong>2010</strong> IEEE.<br />
No.515<br />
Author (s) : Kasi, A.K., Kasi, J.K., Afzulpurkar, N., Bohez, E., Tuantranont, A., Mahaisavariya, B.<br />
Title : Novel anodic aluminum oxide (AAO) nanoporous membrane for wearable hemodialysis device<br />
Source : ICCE <strong>2010</strong> - 3rd <strong>International</strong> Conference on Communications and Electronics, <strong>2010</strong>, Article number 5670689, Pages 98-101<br />
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Document Type : Conference Paper<br />
Keywords : AAO; Hard anodization; Hemodialysis; Mild anodization; Nanoporous membrane<br />
<strong>Abstract</strong> : In this paper a novel structure <strong>of</strong> AAO membrane is reported. The AAO membrane was fabricated from commercial grade<br />
impure aluminum sheet. The fabricated membrane has hexagonal structure and the nanopores appeared at the bottom <strong>of</strong><br />
that honeycomb structure. The thickness <strong>of</strong> fabricated membrane is 42.5μm. The average diameter <strong>of</strong> upper layer pore is<br />
350nm and average diameter <strong>of</strong> lower layer pore is 50nm. The nanopores inside the fabricated membrane are highly straight<br />
and perpendicular to the surface. This two layer AAO membrane will speed up the hemodialysis process. ©<strong>2010</strong> IEEE.<br />
No.516<br />
Author (s) : Katchamart, W., Bourré-Tessier, J., Donka, T., Drouin, J., Rohekar, G., Bykerk, V.P., Haraoui, B., LeClerq, S., Mosher, D.P., Pope,<br />
J.E., Shojania, K., Thomson, J., Thorne, J.C., Bombardier, C., Blocka, K., Flanagan, C.E., Davis, P., Olszynski, W., Ahluwalia, V.,<br />
Zummer, M., Boire, G., Henderson, J., Koehler, B., Baer, P., Ahmad, Z., St-Pierre, A., Cohen, M., Abu-Hakima, M., Khraishi, M.,<br />
Soucy, E., Karsh, J.<br />
Title : Canadian recommendations for use <strong>of</strong> methotrexate in patients with rheumatoid arthritis<br />
Source : Journal <strong>of</strong> Rheumatology, Volume 37, Issue 7, July <strong>2010</strong>, Pages 1422-1430<br />
Document Type : Review<br />
Keywords : Everyday practice; Guideline; Methotrexate; Recommendation; Rheumatoid arthritis; Systematic review<br />
<strong>Abstract</strong> : Objective. To develop recommendations for the use <strong>of</strong> methotrexate (MTX) in patients with rheumatoid arthritis. Methods.<br />
Canadian rheumatologists who participated in the international 3e Initiative in Rheumatology (evidence, expertise, exchange)<br />
in 2007-2008 formulated 5 unique Canadian questions. A bibliographic team systematically reviewed the relevant literature<br />
on these 5 topics. An expert committee consisting <strong>of</strong> 26 rheumatologists from across Canada was convened, and a set <strong>of</strong><br />
recommendations was proposed based on the results <strong>of</strong> systematic reviews combined with expert opinions using a nominal<br />
group consensus process. Results. The 5 questions addressed drug interactions, predictors <strong>of</strong> response, strategies to reduce<br />
non-serious side effects, variables to assess clinical response, and incorporating patient preference into decision-making. The<br />
systematic review retrieved 93 pertinent articles; this evidence was presented to the expert committee during the interactive<br />
workshop. After extensive discussion and voting, a total <strong>of</strong> 9 recommendations were formulated: 2 on drug interactions, 1 on<br />
predictors <strong>of</strong> response, 2 on strategies to reduce non-serious side effects, 3 on variables to assess clinical response, and 1 on<br />
incorporating patient preferences into decision-making. The level <strong>of</strong> evidence and the strength <strong>of</strong> recommendations are<br />
reported. Agreement among panelists ranged from 85% to 100%. Conclusion. Nine recommendations pertaining to the use <strong>of</strong><br />
MTX in daily practice were developed using an evidence-based approach followed by expert/physician consensus with high<br />
level <strong>of</strong> agreement. The Journal <strong>of</strong> Rheumatology Copyright © <strong>2010</strong>. All rights reserved.<br />
No.517<br />
Author (s) : Katchamart, W., Johnson, S., Lin, H.-J.L., Phumethum, V., Salliot, C., Bombardier, C.<br />
Title : Predictors for remission in rheumatoid arthritis patients: A systematic review<br />
Source : Arthritis Care and Research, Volume 62, Issue 8, August <strong>2010</strong>, Pages 1128-1143<br />
Document Type : Article<br />
Keywords : adalimumab, disease modifying antirheumatic drug, etanercept, gold<br />
salt, hydroxychloroquine, infliximab, methotrexate, methylprednisolone, prednisolone, salazosulfapyridine, tumor necrosis<br />
factor antibody; age, article, drug use, human, nuclear magnetic resonance imaging, onset age, outcome<br />
assessment, prediction, prognosis, remission, rheumatoid arthritis, sex difference, smoking, systematic review; Antirheumatic<br />
Agents, Arthritis, Rheumatoid, Haplotypes, Humans, Interleukin-2, Male, Methotrexate, Patient<br />
Selection, Prognosis, Registries, Rheumatoid Factor, Sulfasalazine, Treatment Outcome, Tumor Necrosis Factor-alpha<br />
<strong>Abstract</strong> : Objective. To summarize the potential predictors <strong>of</strong> remission in patients with rheumatoid arthritis (RA). Methods. We<br />
performed a systematic review <strong>of</strong> prognostic studies that identified the predictors <strong>of</strong> remission in RA patients. Studies were<br />
identified in Medline, EMBase, and the Cochrane Registry, and by hand search. We included only studies performing<br />
multivariate analysis. Results. A total <strong>of</strong> 18 studies from 2,062 citations were included. The following variables were found to<br />
be the independent predictors <strong>of</strong> RA remission: male sex; young age; late-onset RA; short disease duration; nonsmoker; low<br />
baseline disease activity; mild functional impairment; low baseline radiographic damage; absence <strong>of</strong> rheumatoid factor and<br />
anti - citrullinated peptide; low serum level <strong>of</strong> acute-phase reactant, interleukin-2, and RANKL at baseline; MTHFR 677T<br />
alleles and 1298C alleles in the methotrexate (MTX) - treated patients; magnetization transfer ratio 2756A allele ± either the<br />
SLC 19A180A allele or the TYMS 3R-del6 haplotype in the MTX plus sulfasalazine combination - treated patients; early<br />
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treatment with nonbiologic disease-modifying antirheumatic drug (DMARD) combinations; the use <strong>of</strong> anti - tumor necrosis<br />
factor (anti-TNF); the concurrent use <strong>of</strong> DMARDs in anti-TNF - treated patients; and moderate or good response to treatments<br />
at the first 6 months. The magnitude <strong>of</strong> the association in the individual predictor was diverse among the studies depending<br />
on the patient characteristics, the study characteristics, and the variables used to adjust for in the models. Conclusion. A<br />
number <strong>of</strong> independent predictors <strong>of</strong> remission, i.e., baseline clinical and laboratory characteristics and genetic markers, were<br />
summarized. The predictive value <strong>of</strong> prognostic factors recently identified needs to be confirmed. © <strong>2010</strong>, American College<br />
<strong>of</strong> Rheumatology.<br />
No.518<br />
Author (s) : Katchamart, W., Trudeau, J., Phumethum, V., Bombardier, C.<br />
Title : Methotrexate monotherapy versus methotrexate combination therapy with non-biologic disease modifying anti-rheumatic<br />
drugs for rheumatoid arthritis.<br />
Source : Cochrane database <strong>of</strong> systematic reviews (Online), Volume 4, <strong>2010</strong>, Pages CD008495<br />
Document Type : Review<br />
Keywords : antirheumatic agent, methotrexate; adult, drug combination, human, meta analysis, methodology, randomized controlled<br />
trial, review, rheumatoid arthritis; Adult, Antirheumatic Agents, Arthritis, Rheumatoid, Drug Therapy,<br />
Combination, Humans, Methotrexate, Randomized Controlled Trials as Topic<br />
<strong>Abstract</strong> : BACKGROUND: Methotrexate (MTX) is among the most effective disease modifying anti-rheumatic drugs (DMARDs) in<br />
rheumatoid arthritis (RA) with less toxicity and better tolerability. OBJECTIVES: To evaluate the efficacy and toxicity <strong>of</strong> MTX<br />
monotherapy compared to MTX combination with non-biologic DMARDs in adult with RA. SEARCH STRATEGY: Trials were<br />
identified in MEDLINE (1950 to 2009), EMBASE (1980 to 2009), the Cochrane Controlled trials Registry (CENTRAL) (up to 2009),<br />
the American and European scientific meeting abstracts 2005-9, the reference lists <strong>of</strong> all relevant studies, letters, and review<br />
articles. SELECTION CRITERIA: Randomized controlled trials comparing MTX monotherapy versus MTX combined with other<br />
non-biologic DMARDs <strong>of</strong> at least 12 weeks <strong>of</strong> trial duration in adult RA patients. DATA COLLECTION AND ANALYSIS: Two<br />
reviewers independently identified eligible studies,extracted the data, and assessed the risk <strong>of</strong> bias <strong>of</strong> relevant studies.The<br />
efficacy analysis was stratified into 3 groups based on previous DMARDs use: DMARD naive, MTX inadequate response, and<br />
non-MTX DMARDs inadequate response. The toxicity analysis was stratified by DMARD combination and pooled across trials<br />
for each combination. Our prespecified primary analysis was based on total withdrawal rates for efficacy or toxicity. MAIN<br />
RESULTS: A total <strong>of</strong> 19 trials (2,025 patients) from 6,938 citations were grouped by the type <strong>of</strong> patients randomised. Trials in<br />
DMARD naive patients showed no significant advantage <strong>of</strong> the MTX combination versus monotherapy; withdrawals for lack <strong>of</strong><br />
efficacy or toxicity were similar in both groups (risk ratio (RR) 1.16, 95% CI.0.70 to 1.93, absolute risk difference(ARD) 5%,<br />
95%CI-3% to 13%). Trials in MTX or non-MTX DMARDs inadequate responder patients also showed no difference in<br />
withdrawal rates between the MTX combo versus mono groups with RR 0.86 95% CI 0.49 to1.51, ARD -2 %, 95% CI-13 % to 8<br />
% and RR 0.75 95% CI 0.41 to 1.35, ARD -10%, 95% CI -31% to 11%, respectively. Significant reductions <strong>of</strong> pain and<br />
improvement in physical function (measured by Health Assessment Questionnaire or HAQ) were found in the MTX<br />
combination group, but only in MTX-inadequate responders (absolute risk difference -9.72%, 95%CI -14.7% to -4.75% for pain<br />
and mean difference (MD) -0.28, 95%CI -0.36 to -0.21 (0-3) for HAQ). AUTHORS' CONCLUSIONS: When the balance <strong>of</strong> efficacy<br />
and toxicity is taken into account, the moderate level <strong>of</strong> evidence from our systematic review showed no statistically<br />
significant advantage <strong>of</strong> the MTX combination versus monotherapy. Trials are needed that compare currently used MTX doses<br />
and combination therapies.<br />
No.519<br />
Author (s) : Katchamart, W., Bombardier, C.<br />
Title : Systematic Monitoring <strong>of</strong> Disease Activity Using an Outcome Measure Improves Outcomes in Rheumatoid Arthritis<br />
Source : AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, Volume: 83, Issue: 5, Supplement: S, Pages: 219-220, Meeting<br />
<strong>Abstract</strong>: 738, Published: NOV <strong>2010</strong><br />
Document Type : Article<br />
Keywords : RHEUMATOID ARTHRITIS; SYSTEMATIC MONITORING; TREATMENT OUTCOME; TIGHT CONTROL STRATEGY; DAILY PRACTICE;<br />
SYSTEMATIC REVIEW<br />
<strong>Abstract</strong> : Objective. To systematically review the literature on the value <strong>of</strong> outcome measures to monitor treatment response in<br />
patients with rheumatoid arthritis (RA). Methods. Canadian rheumatologists participating in the <strong>International</strong> 3e (evidence<br />
expertise exchange) Initiative formulated the question "Which parameters should be recommended for use in the<br />
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management <strong>of</strong> RA patients to assess a clinically meaningful response in clinical practice?". Searches in 3 electronic<br />
databases, Medline, Embase, and Cochrane Central Register <strong>of</strong> Controlled Trials, yielded no relevant study addressing this<br />
question. Experts in the field proposed to extrapolate evidence from 3 randomized controlled trials <strong>of</strong> systematic monitoring<br />
or tight control strategy in the management <strong>of</strong> RA. Results. Three studies were included in this review. The TICORA study<br />
showed that intensive management using systematic monitoring with the Disease Activity Score (DAS) aiming at least low<br />
disease activity, monthly followup, and more aggressive disease-modifying antirheumatic drug (DMARD) treatment improves<br />
outcomes with higher remission rates (65% vs 16%; p < 0.0001). Fransen, et al demonstrated that targeted therapy aimed at<br />
low disease activity (DAS28 < 3.2) led to more changes in DMARD treatment, resulting in a larger number <strong>of</strong> patients with low<br />
disease activity (31% vs 16%; p = 0.028). The CAMERA study showed that systematic monitoring using the objective computer<br />
decision program evaluation and monthly followup yielded a greater remission rate (50% vs 37%; p = 0.0001). Conclusion.<br />
Systematic monitoring <strong>of</strong> disease activity, aiming for at least low disease activity, and frequent followup improves outcome in<br />
RA. (First Release May 1 <strong>2010</strong>; J Rheumatol <strong>2010</strong>; 37:1411-5; doi:10.3899/jrheum.090980)<br />
No.520<br />
Author (s) : Katchanov, J., Nawa, Y.<br />
Title : Helminthic invasion <strong>of</strong> the central nervous system: Many roads lead to Rome<br />
Source : Parasitology <strong>International</strong>, Volume 59, Issue 4, December <strong>2010</strong>, Pages 491-496<br />
Document Type : Review<br />
Keywords : Central nervous system infection; Helminths; Parasitosis<br />
<strong>Abstract</strong> : Invasion <strong>of</strong> the central nervous system (CNS) by parasitic worms <strong>of</strong>ten represents most severe complication <strong>of</strong> human<br />
helminthiasis. The pathways from the portal <strong>of</strong> entry to the CNS are manifold and differ from species to species. In this minireview,<br />
we analysed the contemporary knowledge and current concepts <strong>of</strong> the routes pathogenic helminths take to gain<br />
access to brain, spinal cord and subarachnoid space. © <strong>2010</strong> Elsevier Ireland Ltd.<br />
No.521<br />
Author (s) : Katekaew, P., Veerasai, W., Aeimbhu, A.<br />
Title : Effect <strong>of</strong> micro-arc oxidation time on the Ca-P coating layer properties formed on commercially pure titanium<br />
Source : IFMBE Proceedings, Volume 31 IFMBE, <strong>2010</strong>, Pages 1200-1203<br />
Document Type : Conference Paper<br />
Keywords : Biomaterials; Ca-P coating; Microarc oxidation; Roughness; Titanium<br />
<strong>Abstract</strong> : In the present study, the Ca-P coating layer on commercially pure titanium was fabricated using Micro-arc oxidation (MAO)<br />
method in electrolyte for osteointegrative coating (ESOC) at constant potential for different periods <strong>of</strong> time (0 - 600 minutes).<br />
The morphological microstructures, crystal structure and roughness <strong>of</strong> Ca-P coating layers were characterised by Scanning<br />
Electron Microscopy (SEM), Energy Dispersive X-ray Spectrometer (EDS), X-ray Diffraction technique (XRD) and Atomic Force<br />
Microscope (AFM). SEM micrographs <strong>of</strong> treated samples demonstrated the microporous structure <strong>of</strong> Ca-P coating layer.<br />
Moreover, the results indicated that the number and size <strong>of</strong> micropores have increased with an increasing oxidation time. The<br />
contents <strong>of</strong> Ca and P on coating layer depend on the oxidation time. XRD results showed that the fabricated Ca-P coating<br />
layer contains Ti and hydroxyapatite (HA) phases at t = 600 minutes. AFM analysis showed that a mean roughness <strong>of</strong><br />
untreated was 13 nm and treated samples ranging between 80-190 nm. Wettability property <strong>of</strong> an untreated and treated<br />
sample was hydrophilic which the magnitude was no different. © <strong>2010</strong> <strong>International</strong> Federation for Medical and Biological<br />
Engineering.<br />
No.522<br />
Author (s) : Kato, S, Ohno, T., Thephamongkhol, K., Chansilpa, Y., Yang, YX., Devi, CRB., Bustam, AZ., Calaguas, MJC., de los Reyes, RH.,<br />
Cho, CK., To, AD., Supriana, N., Mizuno, H., Nakano, T., Tsujii, H.<br />
Title : MULTI-INSTITUTIONAL PHASE II CLINICAL STUDY OF CONCURRENT CHEMORADIOTHERAPY FOR LOCALLY ADVANCED CERVICAL<br />
CANCER IN EAST AND SOUTHEAST ASIA<br />
Source : INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS, Volume: 77, Issue: 3, Pages: 751-757, DOI:<br />
10.1016/j.ijrobp.2009.06.011, Published: JUL 1 <strong>2010</strong><br />
Document Type : Article<br />
Keywords : Cervical cancer; chemoradiotherapy; high-dose-rate brachytherapy; developing country; international clinical study<br />
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<strong>Abstract</strong> : Purpose: To evaluate the toxicity and efficacy <strong>of</strong> concurrent chemoradiotherapy using weekly cisplatin for patients with<br />
locally advanced cervical cancer in East and Southeast Asia, a multi-institutional Phase II clinical study was conducted among<br />
eight Asian countries. Methods and Materials: Between April 2003 and March 2006, 120 patients (60 with bulky Stage IIB and<br />
60 with Stage IIIB) with previously untreated squamous cell carcinoma <strong>of</strong> the cervix were enrolled in the present study.<br />
Radiotherapy consisted <strong>of</strong> pelvic external beam radiotherapy (total (lose, 50 Gy) and either high-dose-rate or low-dose-rate<br />
intracavitary brachytherapy according to institutional practice. The planned Point A dose was 24-28 Gy in four fractions for<br />
high-dose-rate-intracavitary brachytherapy and 40-45 Gy in one to two fractions for low-dose-rate-intracavitary brachytherapy.<br />
Five cycles <strong>of</strong> weekly cisplatin (40 mg/m(2)) were administered during the radiotherapy course. Results: All patients were<br />
eligible for the study. The median follow-up was 27.3 months. Of the 120 patients, 100 (83%) received four or five cycles <strong>of</strong><br />
chemotherapy. Acute Grade 3 leukopenia was observed in 21% <strong>of</strong> the patients, and Grade 3 gastrointestinal toxicity was<br />
observed in 6%. No patient failed to complete the radiotherapy course because <strong>of</strong> toxicity. The 2-year local control and<br />
overall survival rate for all patients was 87.1% and 79.6%, respectively. The 2-year major late rectal and bladder complication<br />
rate was 2.5% and 0%, respectively. Conclusion: The results have suggested that concurrent chemoradiotherapy using weekly<br />
cisplatin is feasible and effective for patients with locally advanced cervical cancer in East and Southeast Asia. (C) 2019<br />
Elsevier Inc.<br />
No.523<br />
Author (s) : Kato-Hayashi, N., Kirinoki, M., Iwamura, Y., Kanazawa, T., Kitikoon, V., Matsuda, H., Chigusa, Y.<br />
Title : Identification and differentiation <strong>of</strong> human schistosomes by polymerase chain reaction<br />
Source : Experimental Parasitology, Volume 124, Issue 3, March <strong>2010</strong>, Pages 325-329<br />
Document Type : Article<br />
Keywords : Cox1; Mitochondial DNA; Multiplex; PCR; S. haematobium; S. japonicum; S. mekongi; Schistosoma mansoni<br />
<strong>Abstract</strong> : Recent increasing number <strong>of</strong> travelers, immigrants and foreign workers from schistosomiasis endemic area has thus resulted in<br />
the importation <strong>of</strong> schistosomiasis to non-endemic countries. To avoid ova-induced pathogenicity, sensitive and specific<br />
diagnostic means at an early stage <strong>of</strong> infection are therefore crucial. In this study, we developed polymerase chain reaction<br />
(PCR) primers specific for human schistosome species. The PCR products were obtained in a species-specific manner (479. bp,<br />
Schistosoma mansoni; 365. bp, S. haematobium; 614. bp, S. japonicum; 303. bp, S. mekongi) and were detectable from 0.01.<br />
pg <strong>of</strong> total worm DNA (S. haematobium, S. japonicum, S. mekongi). The primer sets were also available for multiplex use.<br />
Although some difficulties were experienced in amplifying the parasite DNA from the infected animals, schistosome DNA<br />
could be detected from one day post infection. The PCR method described herein will therefore be beneficial to detect<br />
human schistosomiasis, after some improvements in this method. © 2009 Elsevier Inc.<br />
No.524<br />
Author (s) : Katrun, P., Chiampanichayakul, S., Korworapan, K., Pohmakotr, M., Reutrakul, V., Jaipetch, T., Kuhakarn, C.<br />
Title : PhI(OAc)2/KI-mediated reaction <strong>of</strong> aryl sulfinates with alkenes, alkynes, and α,β-unsaturated carbonyl compounds: Synthesis<br />
<strong>of</strong> vinyl sulfones and β-iodovinyl sulfones<br />
Source : European Journal <strong>of</strong> Organic Chemistry, Issue 29, October <strong>2010</strong>, Pages 5633-5641<br />
Document Type : Article<br />
Keywords : Alkenes; Alkynes; Iodine; Sulfur<br />
<strong>Abstract</strong> : (Diacetoxyiodo)benzene [PhI(OAc)2, DIB] was able to promote the reaction <strong>of</strong> sodium aryl sulfinate and potassiumiodide (KI)<br />
with alkenes and alkynes to afford the corresponding vinyl sulfones and β-iodovinyl sulfones, respectively, in good yields.<br />
The salient features <strong>of</strong> this reaction are that it employs a commercially available and environmentally benign hypervalent<br />
iodine(III) reagent, a one-step reaction, a short reaction time, and mild reaction conditions. (Diacetoxyiodo)benzene/KI was<br />
able to promote the reaction <strong>of</strong> sodium aryl sulfinates with alkenes to afford vinyl sulfones in good yields. Alkynes<br />
underwent a similar reaction to give β-iodovinyl sulfones. The present method can be considered to be a convenient and<br />
attractive alternative to the existing methods for the synthesis <strong>of</strong> these intermediates, which are important in organic<br />
chemistry. © <strong>2010</strong> WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.<br />
No.525<br />
Author (s) : Kawami, M., Yumoto, R., Nagai, J., Junyaprasert, V.B., Soonthornchareonnon, N., Patanasethanont, D., Sripanidkulchai, B.-O.,<br />
Mikihisa, T.<br />
Title : Effect <strong>of</strong> thai plant extracts on p-glycoprotein function and viability in paclitaxel-resistant HepG2 cells<br />
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Source : Drug Metabolism and Pharmacokinetics, Volume 25, Issue 2, <strong>2010</strong>, Pages 155-162<br />
Document Type : Article<br />
Keywords : Multidrug resistance; P-glycoprotein (MDR1/ABCB1); Paclitaxel; Paclitaxel-resistant HepG2 cells; Rhodamine 123; Thai plant<br />
extracts<br />
<strong>Abstract</strong> : The effects <strong>of</strong> ethanol extracts from Thai plants on P-glycoprotein (P-gp) function and cell viability were examined using<br />
paclitaxel-resistant HepG2 (PR-HepG2) cells. KP018 from Ellipeiopsis cherrevensis and AT80 from Ancistrocladus tectorius<br />
increased both rhodamine 123, a typical P-gp substrate, and [3H]paclitaxel uptake in PR-HepG2 cells. However, some extracts<br />
such as MT80 from Microcos tomentosa increased rhodamine 123, but not [3H]paclitaxel, uptake, while MM80 from<br />
Micromelum minutum increased only [3H]paclitaxel uptake. Thus, the effects <strong>of</strong> extracts <strong>of</strong> Thai plants on rhodamine 123<br />
uptake were not necessarily the same as those on [3H]paclitaxel uptake. Purified compounds such as bergapten did not<br />
affect the uptake <strong>of</strong> either substrate. KP018, AT80, and MM80 increased [3H]paclitaxel uptake and decreased the cell viability<br />
in a concentration-dependent manner. Among these extracts, KP018 showed the most potent cytotoxicity. The cytotoxic<br />
potency <strong>of</strong> KP018 on PR-HepG2 cells was similar to that on wild-type HepG2 cells, and was not potentiated by verapamil. At<br />
concentrations resulting in no cytotoxicity, AT80 and MM80 potentiated paclitaxel-induced cytotoxicity in PR-HepG2 cells.<br />
These results indicate that K018 may be a useful source to search for a new anticancer drug, while AT80 and MM80 may be<br />
useful as modulators <strong>of</strong> P-gp-mediated multidrug resistance in cancer cells.<br />
No.526<br />
Author (s) : Kayurapan, A., Makadelok, S., Waikakul, S.<br />
Title : Effect <strong>of</strong> gamma sterilisation and deep-freezing on length and strength <strong>of</strong> fascia latae.<br />
Source : Journal <strong>of</strong> orthopaedic surgery (Hong Kong), Volume 18, Issue 1, April <strong>2010</strong>, Pages 68-70<br />
Document Type : Article<br />
Keywords : animal, article, comparative study, cryopreservation, fascia lata, freezing, gamma radiation, instrument sterilization, materials<br />
testing, methodology, pathology, pathophysiology, radiation exposure, swine, tensile strength, tissue culture technique;<br />
Animals, Cryopreservation, Fascia Lata, Freezing, Gamma Rays, Materials Testing, Sterilization, Swine, Tensile Strength, Tissue<br />
Culture Techniques<br />
<strong>Abstract</strong> : PURPOSE: To compare the length and strength <strong>of</strong> fascia latae after gamma sterilisation and different durations <strong>of</strong> deepfreezing.<br />
METHODS: 50 pieces <strong>of</strong> fresh porcine fascia latae were randomly divided into 5 groups. Group 1 acted as controls,<br />
which were not gamma irradiated and deep-frozen. In groups 2 to 4, fascia latae were incubated in phosphate buffer solution<br />
for 4 hours, and then gamma irradiated at 25 kGy. They were preserved at -70 degrees Celcius for one to 3 months,<br />
respectively. In group 5, fascia latae were preserved for 3 months, and during the whole process they were fixed on a<br />
wooden board to maintain their original length. The maximum tensile strength <strong>of</strong> each fascia lata was tested at a<br />
displacement rate <strong>of</strong> 1 cm per minute until failure. RESULTS: The maximum tensile strength was not significantly different<br />
among groups 1 to 4, but was significantly higher in group 5. CONCLUSION: Gamma sterilisation and deep-freezing had no<br />
effect on the strength <strong>of</strong> fascia latae, but fixation on a board could increase strength.<br />
No.527<br />
Author (s) : Kc, S.; Bhuju, G. B.; Tragulpiankit, P.<br />
Title : Pattern <strong>of</strong> Adverse Drug Reactions Reported by Nepal Regional Pharmacovigilance Centers<br />
Source : DRUG SAFETY, Volume: 33, Issue: 10, Pages: 940-940, Meeting <strong>Abstract</strong>: 109, Published: <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.528<br />
Author (s) : Keeratichamroen, W., Dechsri, P., Panijpan, B., Ruenwongsa, P.<br />
Title : Enhancing student conceptualization <strong>of</strong> a combustion chemical reaction using the tapioca bomb activity: An inquiry-based<br />
approach<br />
Source : <strong>International</strong> Journal <strong>of</strong> Learning, Volume 17, Issue 1, <strong>2010</strong>, Pages 275-292<br />
Document Type : Article<br />
Keywords : Chemical reaction; Combustion reaction; Constructivist; Inquiry-based approach; Learning unit<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : At the microscopic level, there are many concepts that appear too complicated to all grades <strong>of</strong> students; especially those<br />
concerning chemical reactions. Most students have difficulty in imagining things and understanding the relevant concepts. In<br />
science, activity-based learning is deemed an appropriate method to improve students' understanding <strong>of</strong> complicated<br />
concepts. The purpose <strong>of</strong> this study was to develop an inquiry-based learning unit on chemical reaction for grade eight<br />
students by using a newly developed activity called "Tapioca Bomb" for demonstrating concepts <strong>of</strong> chemical reaction. The<br />
students had opportunities to investigate by hands-on and mind-on activities. After observing the explosion in the Tapioca<br />
Bomb activity, the students were able to build their own concepts on chemical reaction and connect science learning to<br />
everyday life. The students could explain how and why the reaction occurred, and could apply their preliminary knowledge<br />
to design their own experiment. Finally, the teacher and students discussed what they had learned and applied the<br />
knowledge gained. The results on pre-test and post-test, concept map, students' documents, CLES questionnaire and<br />
interviews indicated that this learning unit helped students conceptualize the knowledge on combustion chemical reaction. ©<br />
Common Ground, Wasana Keeratichamroen, Precharn Dechsri, Bhinyo Panijpan, Pintip Ruenwongsa.<br />
No.529<br />
Author (s) : Keeratichananont, S., Sobhonslidsuk, A., Kitiyakara, T., Achalanan, N., Soonthornpun, S.<br />
Title : The role <strong>of</strong> liquid simethicone in enhancing endoscopic visibility prior to Esophagogastroduodenoscopy (EGD): A prospective,<br />
randomized, double-blinded, placebo-controlled trial<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, 93 (8), pp. 892-897.<br />
Document Type : Article<br />
Keywords : Endoscopic visibility; Esophagogastroduodenoscopy; Simethicone<br />
<strong>Abstract</strong> : Background: Simethicone improves endoscopic visibility and diagnostic accuracy during colonoscopy and capsule endoscopy.<br />
Nevertheless, there have been limited data on its usefulness in esophagogastroduodenoscopy (EGD). Objective: To evaluate<br />
the effectiveness <strong>of</strong> simethicone on enhancing endoscopic visibility in patients undergoing EGD. Material and Method: 121<br />
patients were randomized to take 2 ml <strong>of</strong> either liquid simethicone or placebo in 60 ml <strong>of</strong> water at 15-30 minutes before<br />
EGD. The severity scores <strong>of</strong> foam and bubbles at the esophagus, stomach and duodenum were compared. Results:<br />
Simethicone improved endoscopic visibility by diminishing mean cumulative (6.83±2.4 vs. 11.05 ± 2.6, p < 0.001) and local<br />
scores <strong>of</strong> foam and bubbles at all areas, and decreased the number and timing <strong>of</strong> adjunctive simethicone washing (17.5% vs.<br />
74.1%, p < 0.001 and 0 vs. 19 seconds, p < 0.001). Simethicone increased endoscopist and patient satisfaction significantly<br />
without having adverse effects. Conclusion: Using simethicone before EGD enhances endoscopic visibility, reduces adjunctive<br />
simethicone washing and increases endoscopist and patient satisfaction.<br />
No.530<br />
Author (s) : Kent, S.J., Cooper, D.A., Vun, M.C., Shao, Y., Zhang, L., Ganguly, N., Bela, B., Tamashiro, H., Ditangco, R., Rerks-Ngarm, S.,<br />
Pitisuttithum, P., van Kinh, N., Bernstein, A., Osmanov, S.<br />
Title : AIDS Vaccine for Asia Network (AVAN): Expanding the regional role in developing HIV vaccines<br />
Source : PLoS Medicine, Volume 7, Issue 9, September <strong>2010</strong>, Article number e1000331<br />
Document Type : Article<br />
Keywords : alvac, DNA vaccine, glycoprotein gp 120, Human immunodeficiency virus vaccine, peptide vaccine, unclassified drug, V3<br />
peptide vaccine, virus vector; acquired immune deficiency syndrome, article, Australia, China, clinical trial, drug efficacy, drug<br />
research, drug safety, geographic distribution, good manufacturing practice, health behavior, health promotion, high risk<br />
population, human, Human immunodeficiency virus infection, India, infection risk, intravenous drug abuse, Japan, male<br />
homosexual, medical ethics, morbidity, nonhuman, pandemic, prevalence, prostitution, strategic planning, Thailand, unsafe<br />
sex, vaccine production, viral gene delivery system, viral gene therapy, virus transmission, Asia, clinical trial<br />
(topic), health, immunology, international cooperation, medical research; AIDS Vaccines, Asia, Biomedical Research, Clinical<br />
Trials as Topic, HIV Infections, Humans, <strong>International</strong> Cooperation, Pandemics, World Health<br />
<strong>Abstract</strong> : –<br />
No.531<br />
Author (s) : Keorochana, G., Johnson, J.S., Taghavi, C.E., Liao, J.-C., Lee, K.-B., Yoo, J.H., Ngo, S.S., Wang, J.C.<br />
Title : The effect <strong>of</strong> needle size inducing degeneration in the rat caudal disc: Evaluation using radiograph, magnetic resonance<br />
imaging, histology, and immunohistochemistry<br />
Source : Spine Journal, Volume 10, Issue 11, November <strong>2010</strong>, Pages 1014-1023<br />
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Document Type : Article<br />
Keywords : Cellular and matrix changes; Different needle sizes; Disc degeneration; Needle puncture; Rat caudal disc<br />
<strong>Abstract</strong> : Background context: The rat caudal disc has been increasingly used in studying <strong>of</strong> disc degeneration because <strong>of</strong> its simplicity,<br />
low cost, and efficiency. However, the reproducibility and standardization are essential to facilitate the investigations <strong>of</strong><br />
biologic therapeutics at different stages <strong>of</strong> degeneration. Purpose: To identify the effect <strong>of</strong> different needle gauges to the<br />
degenerative response in rat caudal discs and to examine its pathogenesis by looking at the cellular and matrix changes.<br />
Study design: In vivo study <strong>of</strong> injury-induced rat caudal disc degeneration using needle puncture. Patient sample: Thirty-six<br />
Lewis rats aged 12-14 weeks. Outcome measures: The induced degenerative discs were analyzed by plain radiograph,<br />
magnetic resonance imaging (MRI) and histological examination. Proteoglycan content was assessed by alcian blue stain.<br />
Immunohistochemistry using aggrecan, collagen II, and Sox-9 was also evaluated to investigate cell differentiation and matrix<br />
changes. Methods: All rats were divided into three groups according to different needle gauges (18G, 20G, and 22G). Caudal<br />
discs were punctured percutaneously under image guidance. Radiographs and MRI were obtained at 2 weeks interval until 8<br />
weeks. At each time point, three rats from each group were sacrificed for histological analysis and immunohistochemistry.<br />
Results: Larger needle gauges, especially 18G, produced more deterioration <strong>of</strong> the disc when compared with smaller sizes,<br />
particularly with time. Significant differences were identified in almost all parameters compared between 18G and 22G at the<br />
8-week time point. For the effect <strong>of</strong> time in the same needle size, the differences occurred between 2- or 4-week and 8-week<br />
time point in the 18G and 20G groups. The proteoglycan and aggrecan stain gradually decreased over time. Chondrogenic<br />
differentiation was identified within the degenerative disc by detecting Sox-9 positive cells and collagen II accumulation<br />
increased as degeneration progressed. Conclusions: The puncture-induced degenerative changes in rat caudal discs can<br />
imitate the human degenerative cascade as observed in plain radiograph, MRI, histology, and immunohistochemistry. We<br />
suggest that needle size affects the occurrence <strong>of</strong> progression <strong>of</strong> degeneration; thus, the large needle size was required to<br />
accelerate the deterioration. The size <strong>of</strong> needle and time point after injury should be considered when investigating the<br />
effect <strong>of</strong> therapeutic materials to retard degeneration or regenerate the intervertebral disc. © <strong>2010</strong> Elsevier Inc. All rights<br />
reserved.<br />
No.532<br />
Author (s) : Keorochana, G., Taghavi, C.E., Tzeng, S.-T., Lee, K.-B., Liao, J.-C., Yoo, J.H., Wang, J.C.<br />
Title : MRI classification <strong>of</strong> interspinous ligament degeneration <strong>of</strong> the lumbar spine: Intraobserver and interobserver reliability and<br />
the frequency <strong>of</strong> disagreement<br />
Source : European Spine Journal, Volume 19, Issue 10, October <strong>2010</strong>, Pages 1740-1745<br />
Document Type : Article<br />
Keywords : Classification; Degeneration; Interspinous ligament; Lumbar spine; Magnetic resonance imaging; Reliability<br />
<strong>Abstract</strong> : Posterior spinal ligament pathology is becoming increasingly recognized as a significant cause <strong>of</strong> low back pain. Despite the<br />
growing clinical importance <strong>of</strong> interspinous ligament degeneration in low back pain patients, formal reliability studies for the<br />
magnetic resonance imaging (MRI) evaluation <strong>of</strong> interspinous ligaments have not been performed. We proposed an MRI<br />
classification system for interspinous ligament degeneration and conducted a comprehensive reliability and reproducibility<br />
assessment. Fifty patients who had low back pain with or without leg discomfort (26 males and 24 females) with a mean age<br />
<strong>of</strong> 48.8 years (range 23-85 years) were studied. The classification for lumbar interspinous ligament degeneration was<br />
developed on the basis <strong>of</strong> the literature using mid-sagittal T1- and T2-weighted images. Three spine surgeons independently<br />
graded a total <strong>of</strong> 200 interspinous ligament levels. Intraobserver and interobserver reliability were assessed by kappa statistics.<br />
The frequency <strong>of</strong> disagreement was also identified. The intraobserver agreement was excellent in all readers (kappa range<br />
0.840-0.901). The interobserver agreement was lower as expected, and was substantial to excellent (kappa range 0.726-0.818).<br />
Overall complete agreement was obtained in 87.8% <strong>of</strong> all interspinous ligament levels. A difference <strong>of</strong> 1, 2, and 3 grades<br />
occurred in 8.1, 3.0, and 1.1% <strong>of</strong> readings, respectively. This proposed MRI classification <strong>of</strong> interspinous ligament degeneration<br />
was simple, reliable, and reproducible. Its use as a standardized nomenclature in clinical and radiographic research may be<br />
recommended. © <strong>2010</strong> The Author(s).<br />
No.533<br />
Author (s) : Keorochana, G., Taghavi, C.E., Tzeng, S.-T., Morishita, Y., Yoo, J.H., Lee, K.-B., Liao, J.-C., Wang, J.C.<br />
Title : Magnetic resonance imaging grading <strong>of</strong> interspinous ligament degeneration <strong>of</strong> the lumbar spine and its relation to aging, spinal<br />
degeneration, and segmental motion: Clinical article<br />
Source : Journal <strong>of</strong> Neurosurgery: Spine, Volume 13, Issue 4, October <strong>2010</strong>, Pages 494-499<br />
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Document Type : Article<br />
Keywords : Degeneration; Interspinous ligament; Lumbar spine; Magnetic resonance imaging; Reliability; Segmental motion<br />
<strong>Abstract</strong> : Object. Degenerative changes <strong>of</strong> the interspinous ligaments (ISLs) have generally been ignored in previous studies. Factorrelated<br />
causes, the effects that these changes have on other structures within the spinal functional unit, and their relation to<br />
kinematic changes in the spine are lacking. In this study, the authors evaluated the reliability <strong>of</strong> a proposed MR imaging<br />
grading system <strong>of</strong> ISL degeneration (ISLD). They also investigated the relationship between ISLD and aging, disc/facet joint<br />
degeneration, and lumbar segmental motion. Methods. The authors studied 256 lumbar motion segments from L-2 to S-1 in<br />
64 patients (35 men and 29 women) with a mean age <strong>of</strong> 46.08 years (range 23-85 years). An MR imaging-based grading system<br />
for ISLD was developed and ranged from Grade A (mild) to Grade D (severe). The reliability was tested, and the correlation <strong>of</strong><br />
the grade with the severity <strong>of</strong> the disc and facet joint degeneration was examined. The segmental motion <strong>of</strong> each functional<br />
unit was measured using flexion/extension MR imaging, and their relationships with ISL grades were identified. Results. Grade<br />
A was observed in 115 levels (44.9%), Grade B in 105 (41.0%), Grade C in 15 (5.9%), and Grade D in 21 levels (8.2%). The<br />
kappa coefficients for intraobserver and interobserver agreements were substantial to excellent (intraobserver [0.871] and<br />
interobserver [0.721-0.807]). Grade D was observed primarily in elderly patients. Segmental motion tended to decrease in the<br />
most severe grade, with a significant difference in angular mobility. As the severity <strong>of</strong> ISLD increased, the severity <strong>of</strong> disc/facet<br />
joint degeneration increased (p < 0.001 and p < 0.05, respectively). Conclusions. The authors proposed a reliable and<br />
reproducible grading system that may be used to investigate spinal kinematics in association with ISLD. The authors' findings<br />
illustrated the distribution <strong>of</strong> ISLD grades. The most severe grade occurred primarily in elderly patients. Mobility decreased in<br />
the most severe grade; therefore, the stage <strong>of</strong> ISLD should be taken into consideration when evaluating spinal stability.<br />
No.534<br />
Author (s) : Keorochana, G., Tawonsawatruk, T., Laohachareonsombat, W., Wajanavisit, W., Jaovisidha, S.<br />
Title : The results <strong>of</strong> decompression and instrumented fusion with pedicular screw plate system in degenerative lumbar scoliosis<br />
patients with spinal stenosis: A prospective observational study<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 4, April <strong>2010</strong>, Pages 457-461<br />
Document Type : Article<br />
Keywords : Arthrodesis; Degenerative scoliosis; Instrumentation; Outcome; Spinal stenosis; Surgery<br />
<strong>Abstract</strong> : Objective: To study pain relief, curve correction, and functional outcomes after decompression and instrumented arthrodesis<br />
in degenerative lumbar scoliosis patients with spinal stenosis. Material and Method: Thirty-one degenerative scoliosis patients<br />
with symptom <strong>of</strong> spinal stenosis who underwent decompression and fusion with pedicular screw plate instrumentation and<br />
completed at least 2-year follow-up were included in this study. Functional outcomes were obtained using Oswestry<br />
Disability Index (ODI) and Roland Morris score. The VAS pain relief, walking ability, and curve correction were collected as a<br />
result <strong>of</strong> the surgery. Results: Average age <strong>of</strong> the patients at the time <strong>of</strong> surgery was 64.87 years (range, 50-87 years) and<br />
mean follow-up was 32 months (range, 24-60 months). There was significant improvement in all outcome parameters<br />
including pain scales, walking ability, ODI, and Roland Morris score (p < 0.05) Five patients had major complications with two<br />
cases requiring re-operation. Conclusion: Degenerative scoliosis with spinal stenosis is a complex spinal disease that requires<br />
decompression and stabilization procedures. Wide decompressive laminectomy and instrumented arthrodesis with pedicular<br />
screw plate system provided satisfied outcome included pain relief and functional restoration.<br />
No.535<br />
Author (s) : Kerddonfak, S., Manuyakorn, W., Kamchaisatian, W., Sasisakulporn, C., Teawsomboonkit, W., Benjaponpitak, S.<br />
Title : The stability and sterility <strong>of</strong> epinephrine prefilled syringe<br />
Source : Asian Pacific Journal <strong>of</strong> Allergy and Immunology, Volume 28, Issue 1, March <strong>2010</strong>, Pages 53-57<br />
Document Type : Article<br />
Keywords : adrenalin; anaphylaxis, article, concentration (parameters), drug potency, drug stability, drug sterility, evaluation, high<br />
performance liquid chromatography, nonhuman, pH, syringe; Anaphylaxis, Capsules, Colony Count, Microbial, Drug<br />
Stability, Drug Storage, Epinephrine, Feasibility Studies, Hot Temperature, Humans, Pharmaceutical Solutions, Practice<br />
Guidelines as Topic, Self Administration, Sterilization, Syringes, Time Factors<br />
<strong>Abstract</strong> : The commercially available auto-injector epinephrine is considerable expensive. Epinephrine prefilled syringe is an alternative<br />
treatment for anaphylaxis patients. The objective <strong>of</strong> the present study was to evaluate the stability and sterility <strong>of</strong><br />
epinephrine prefilled syringe. Epinephrine prefilled syringe was kept in the pencil box to prevent from light exposure. The<br />
active ingredients, integrity and level <strong>of</strong> potency were measured by high-performance liquid chromatography (HPLC). The<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
sterility was accessed by aerobic bacteria and fungi culture. The epinephrine concentration at 1, 2 and 3 months after the<br />
preparation was 101.36, 99.31 and 101.09%, respectively (acceptable range 90 - 110%). The pH was 3.17 - 3.23 (acceptable<br />
range 2.8 - 3.6). Nor-epinephrine was undetected. The cultures for bacteria and fungus were both negative. Consequently,<br />
epinephrine prefilled syringe was stable and sterile at least three month after preparation. Epinephrine prefilled syrine is an<br />
alternative low cost treatment for anaphylaxis patient.<br />
No.536<br />
Author (s) : Kerschbaumer, G., Wernsdorfer, G., Wiedermann, U., Congpuong, K., Sirichaisinthop, J., Wernsdorfer, W.H.<br />
Title : Synergism between mefloquine and artemisinin and its enhancement by retinol in Plasmodium falciparum in vitro<br />
Source : Wiener Klinische Wochenschrift, Volume 122, Issue SUPPL. 3, October <strong>2010</strong>, Pages 57-60<br />
Document Type : Article<br />
Keywords : Artemisinin; Mefloquine; Plasmodium falciparum; Retinol; Synergism<br />
<strong>Abstract</strong> : Following the advent <strong>of</strong> mefloquine resistance in Plasmodium falciparum in Thailand in the 1990s, the combined treatment<br />
<strong>of</strong> falciparum malaria with artesunate and mefloquine was found to be highly effective in treating and curing the patients in<br />
the affected areas. Monitoring <strong>of</strong> the clinical-parasitological response and <strong>of</strong> the in vitro sensitivity <strong>of</strong> P. falciparum was<br />
systematically conducted in order to detect any signs <strong>of</strong> failure <strong>of</strong> this type <strong>of</strong> artemisinin-based combination treatment<br />
(ACT). In earlier observations the in vitro activity <strong>of</strong> artemisinin was found to be significantly enhanced when combined with<br />
retinol. The same applies to mefloquine. In order to check whether the synergism between artemisinin and mefloquine was<br />
maintained in the presence <strong>of</strong> retinol, the pharmacodynamic interaction <strong>of</strong> the three compounds was investigated in the<br />
western border area <strong>of</strong> Thailand. Successful parallel tests with mefloquine, artemisinin, retinol, mefloquine-artemisinin 5:1 as<br />
well as mefloquine-artemisinin (5:1) + retinol low, medium and high were obtained with 43 fresh parasite isolates. The retinol<br />
concentrations in the low, medium and high formulations corresponded to the 50th, 65th and 80th percentile <strong>of</strong> the<br />
physiological mean concentrations in the blood <strong>of</strong> healthy adults. The IC50, IC90 and IC99 values for mefloquine alone<br />
showed a further increase over the data <strong>of</strong> 2008. In the combinations with artemisinin and retinol moderate synergism was<br />
observed at the IC50, but synergism increased strongly at the IC90 and the IC99. © <strong>2010</strong> Springer-Verlag.<br />
No.537<br />
Author (s) : Kestsumpun, Y., Ngamprarsert, M., Prasungsit, C., Anuratpanich, L.<br />
Title : USING SIX SIGMA METHODOLOGY TO REDUCE THE NUMBER OF PATIENT WITH SKIN DAMAGE IN INCONTINENCE PATIENT<br />
Source : JOURNAL OF WOUND OSTOMY AND CONTINENCE NURSING, Volume: 37, Issue: 3, Supplement: S, Pages: S8-S8, Published:<br />
MAY-JUN <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.538<br />
Author (s) : Ketefian, S., Dai, Y.-T., Hanucharurnkul, S., Mendes, I.A.C., Norman, I.J.<br />
Title : Environments for nursing scholarship and journal impact factor in five countries<br />
Source : <strong>International</strong> Nursing Review, Volume 57, Issue 3, September <strong>2010</strong>, Pages 343-351<br />
Document Type : Article<br />
Keywords : Brazil; Environments for Nursing Scholarship; Impact factor; Nursing science; <strong>Publications</strong>; Taiwan; Thailand; United<br />
Kingdom; United States <strong>of</strong> America<br />
<strong>Abstract</strong> : Background: Universities worldwide are seeking objective measures for the assessment <strong>of</strong> their faculties' research products to<br />
evaluate them and to attain prestige. Despite concerns, the impact factors (IF) <strong>of</strong> journals where faculties publish have been<br />
adopted. Research objective: The study aims to explore conditions created within five countries as a result <strong>of</strong> policies<br />
requiring or not requiring faculty to publish in high IF journals, and the extent to which these facilitated or hindered the<br />
development <strong>of</strong> nursing science. Design: The design was a multiple case study <strong>of</strong> Brazil, Taiwan, Thailand (with IF policies,<br />
Group A), United Kingdom and the United States (no IF policies, Group B). Key informants from each country were identified<br />
to assist in subject recruitment. Methods: A questionnaire was developed for data collection. The study was approved by a<br />
human subject review committee. Five faculty members <strong>of</strong> senior rank from each country participated. All communication<br />
occurred electronically. Findings: Groups A and B countries differed on who used the policy and the purposes for which it was<br />
used. There were both similarities and differences across the five countries with respect to hurdles, scholar behaviour,<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
publishing locally vs. internationally, views <strong>of</strong> their science, steps taken to internationalize their journals. Conclusions: In group<br />
A countries, Taiwan seemed most successful in developing its scholarship. Group B countries have continued their scientific<br />
progress without such policies. IF policies were not necessary motivators <strong>of</strong> scholarship; factors such as qualified nurse<br />
scientists, the resource base in the country, may be critical factors in supporting science development. © <strong>2010</strong> The Authors.<br />
<strong>International</strong> Nursing Review © <strong>2010</strong> <strong>International</strong> Council <strong>of</strong> Nurses.<br />
No.539<br />
Author (s) : Ketpichainarong, W., Jittam, P., Ruenwongsa, P., Panijpan, B.<br />
Title : Addressing widespread iodine deficiency disorders: A serious health problem in thailand and beyond<br />
Source : Journal <strong>of</strong> Chemical Education, Volume 87, Issue 7, <strong>2010</strong>, Pages 662-664<br />
Document Type : Article<br />
Keywords : Administrative issues; Applications <strong>of</strong> chemistry; CLIC; Continuing education; General public; Interdisciplinary/Multidisciplinary;<br />
Misconceptions/Discrepant Events; Public understanding/outreach; Textbooks/reference books<br />
<strong>Abstract</strong> : Iodine is the heaviest <strong>of</strong> all elements essential for human health. Iodine is a constituent <strong>of</strong> the thyroid hormone that acts on<br />
many body systems, and thus, iodine's effects are quite broad. In discussing iodine deficiency disorders (IDDs), one should<br />
rank mental defects and physical impairments ahead <strong>of</strong> goiter, which is a mere physical disfigurement. Consuming iodide-or<br />
iodate-supplemented salt has been the most widely implemented public health measure for people at risk <strong>of</strong> iodine<br />
deficiency. However, millions <strong>of</strong> people on several continents still suffer today from insufficient iodine intake. Inadequate<br />
government enforcement and limited access to supplemented salt are partly responsible for the persistence <strong>of</strong> this disease.<br />
Traditional beliefs and food habits are also contributing factors toward difficulties in lowering the incidence and severity <strong>of</strong><br />
IDDs. In the multistep process needed for making dry salt, most iodide in seawater is converted to iodine, which sublimates,<br />
thus drastically reducing the iodine content. Regular consumption <strong>of</strong> iodized table salt is a necessity for people, especially<br />
those at risk <strong>of</strong> developing IDDs. Copyright © <strong>2010</strong> The American Chemical Society and Division <strong>of</strong> Chemical Education, Inc.<br />
No.540<br />
Author (s) : Ketpichainarong, W., Panijpan, B., Ruenwongsa, P.<br />
Title : Enhanced learning <strong>of</strong> biotechnology students by an inquiry-based cellulase laboratory [Biyoteknoloji öǧrencilerinin<br />
sorgulayi{dotless}ci{dotless} temelli selülaz laboratuari{dotless} ile geliştirilmiş öǧrenmeleri]<br />
Source : <strong>International</strong> Journal <strong>of</strong> Environmental and Science Education, Volume 5, Issue 2, <strong>2010</strong>, Pages 169-187<br />
Document Type : Article<br />
Keywords : Biotechnology; Cellulase enzyme; Inquiry; Laboratory; Undergraduate student<br />
<strong>Abstract</strong> : This study explored the effectiveness <strong>of</strong> an inquiry-based cellulase laboratory unit in promoting inquiry in undergraduate<br />
students in biotechnology. The following tools were used to assess the students' achievements and attitude: conceptual<br />
understanding test, concept mapping, students' documents, CLES questionnaire, students' self reflection, and interviews.<br />
Judging from their conceptual understanding test results and concept mapping, students gained significantly more content<br />
knowledge on enzyme-substrate interaction and its application. In addition, students' reports on their projects revealed that<br />
they have developed their critical thinking, scientific process skills and abilities to apply knowledge on enzyme cellulase to<br />
industrial application. The students reacted positively to this teaching strategy as demonstrated by results from questionnaire<br />
responses, students' self reflection and interviews. The success <strong>of</strong> this inquiry-based laboratory unit might be due to both the<br />
context which was <strong>of</strong> interest to students, and the instruction method which ranged from a guided to a more open inquiry.<br />
Most importantly, the teacher in this study had mastery <strong>of</strong> both content and pedagogical techniques. This inquiry-based<br />
cellulase laboratory unit provided significant benefits for teaching and learning science for biotechnology students. It<br />
promoted acquisition <strong>of</strong> content knowledge and skills such as asking good questions, predicting, problem solving, drawing<br />
conclusion, and communication. This inquiry-based laboratory unit may serve as a guideline or framework for implementing a<br />
dynamic instruction with a range <strong>of</strong> inquiry level for the undergraduates. © <strong>2010</strong> IJESE.<br />
No.541<br />
Author (s) : Khaisombat, N.; Saokaew, S.; Chaiyakunapruk, N.; Nathisuwan, S.<br />
Title : ATTRIBUTABLE COST AND LENGTH OF STAY FOR PATIENTS WITH ENOXAPARIN-ASSOCIATED BLEEDING<br />
Source : VALUE IN HEALTH, Volume: 13, Issue: 7, Pages: A506-A506, Published: NOV <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
199
<strong>Abstract</strong> : –<br />
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.542<br />
Author (s) : Khanobdee, K.; Wongprasert, K.; Kitiyanant, Y.<br />
Title : In vitro protection against hydrogen peroxide-induced oxidative stress and cell death in ARPE-19 cells by Curcumin<br />
Source : PLANTA MEDICA, Volume: 76, Issue: 12, Pages: 1242-1242, Published: AUG <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.543<br />
Author (s) : Khompurngson, K., Poltem, D., Yamarat, A., Novaprateep, B.<br />
Title : The use <strong>of</strong> hide in learning the value <strong>of</strong> a function<br />
Source : <strong>International</strong> Conference on Applied Computer Science – Proceedings, <strong>2010</strong>, Pages 193-197<br />
Keywords : Convex optimization and noise data; Hypercircle inequality; Regularization; Reproducing kernel Hilbert space<br />
Document Type : Conference Paper<br />
<strong>Abstract</strong> : In this paper, we briefly review some recent work on Hypercircle inequality for data error (Hide) measured with square loss.<br />
We provide it in the case that the unit ball B is replaced by δB where δ is any positive number. We study the problem in<br />
learning the value <strong>of</strong> a function in reproducing kernel Hilbert space (RKHS) by using the available material from Hide with<br />
different values <strong>of</strong> δ. Moreover, we compare our numerical experiment to the method <strong>of</strong> regularization, which is the<br />
standard method for learning problem. We also discuss the effect <strong>of</strong> the values <strong>of</strong> δ on the learning task under<br />
consideration.<br />
No.544<br />
Author (s) : Khositseth, A., Siripornpitak, S., Laohakunakorn, P.<br />
Title : Hypoplastic right lung associated with right pulmonary vein stenosis and systemic collateral<br />
Source : Congenital Heart Disease, Volume 5, Issue 1, January <strong>2010</strong>, Pages 76-80<br />
Keywords : Hypoplastic right lung; Hypoplastic right pulmonary artery; Pulmonary vein stenosis<br />
<strong>Abstract</strong> : We present a case <strong>of</strong> a 6-month-old girl diagnosed as unilateral pulmonary vein stenosis (right inferior pulmonary vein) with<br />
dextroposition <strong>of</strong> the heart, hypoplastic right lung, hypoplastic right pulmonary artery, collateral from the descending aorta<br />
supplied right lower lung, connection between collateral and right pulmonary artery with retrograde flow from right<br />
pulmonary artery into main pulmonary artery, and right superior and middle pulmonary vein agenesis. A combined modality<br />
<strong>of</strong> imaging including chest film, echocardiography, multidector computerized tomography, and the cardiac catheterization<br />
nicely demonstrated all <strong>of</strong> the unique and rare congenital abnormalities. © <strong>2010</strong> Copyright the Authors. Journal Compilation<br />
© <strong>2010</strong> Wiley Periodicals, Inc.<br />
No.545<br />
Author (s) : Khositseth, A., Siripornpitak, S., Pornkul, R.<br />
Title : Pulmonary atresia and ventricular septal defect with collaterals to right lung associated with anomalous left pulmonary artery<br />
from the ascending aorta<br />
Source : Pediatric Radiology, Volume 40 Suppl 1, December <strong>2010</strong>, Pages S72-76<br />
Document Type : Article<br />
Keywords : Congenital heart disease; Cyanosis; Hemitruncus; Infant; Multidetector CT<br />
<strong>Abstract</strong> : We present a 10-month-old boy with cyanosis. This is a rare case <strong>of</strong> pulmonary atresia, ventricular septal defect (VSD), major<br />
aorto-pulmonary collateral arteries (MAPCAs) to the right lung with absent native right pulmonary artery (RPA) in association<br />
with anomalous left pulmonary artery (LPA) from the ascending aorta (AAo). Echocardiography was unable to identify all <strong>of</strong><br />
the cardiovascular abnormalities. Multidetector CT demonstrated all <strong>of</strong> these abnormalities and is the investigation <strong>of</strong> choice<br />
instead <strong>of</strong> cardiac catheterization. © <strong>2010</strong> Springer-Verlag.<br />
No.546<br />
Author (s) : Khumsiri, N., Jindal, R., Yoswathana, N., Jonglertjunya, W.<br />
Title : Degradation <strong>of</strong> humic acid in soil aqueous extract using the fenton reaction and a microbiological technique<br />
200
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Source : Kasetsart Journal - Natural Science, Volume 44, Issue 6, November <strong>2010</strong>, Pages 1069-1078<br />
Document Type : Article<br />
Keywords : Fenton reaction; Humic acid; Lactococcus lactis; Pseudomonas fluorescens<br />
<strong>Abstract</strong> : In this study, the degradation <strong>of</strong> humic acid (HA) extracted from soil in aqueous solution was carried out using the Fenton<br />
reaction and a microbiological technique. The Fenton reaction produced hydroxyl radicals (•OH) from the catalytic<br />
decomposition <strong>of</strong> hydrogen peroxide (H2O2) by the soluble ferrous ion (Fe2+) for use in the destruction <strong>of</strong> organic pollutants.<br />
At pH 3.0, the ratios <strong>of</strong> HA and H2O2 <strong>of</strong> 1:560 and the H2O2 and Fe2+ ratios <strong>of</strong> 5:1 showed the maximum percentage <strong>of</strong> HA<br />
removal. Thus, the optimal ratio <strong>of</strong> HA:H2O2:Fe2+ for HA removal appeared to be 1:560:112. However, the highest HA<br />
removal per gram <strong>of</strong> soil was 30% after 3 h when using a ratio <strong>of</strong> humic acid to reagents (H2O2 and Fe2+) <strong>of</strong> 1:11200:2240.<br />
The comparative evaluation <strong>of</strong> two microorganisms Pseudomonas fluorescens and Lactococcus lactis for the degradation <strong>of</strong><br />
HA indicated the highest removal <strong>of</strong> about 28% when using Lactococcus lactis as a single culture for soil samples within 20 d.<br />
No.547<br />
Author (s) : Khunawattanakul, W., Puttipipatkhachorn, S., Rades, T., Pongjanyakul, T.<br />
Title : Chitosan-magnesium aluminum silicate nanocomposite films: Physicochemical characterization and drug permeability<br />
Source : <strong>International</strong> Journal <strong>of</strong> Pharmaceutics, Volume 393, Issue 1-2, June <strong>2010</strong>, Pages 220-230<br />
Document Type : Article<br />
Keywords : Chitosan; Drug permeability; Heat treatment; Magnesium aluminum silicate; Nanocomposite film<br />
<strong>Abstract</strong> : Chitosan-magnesium aluminum silicate (CS-MAS) films were prepared and the effects <strong>of</strong> MAS content and heat treatment <strong>of</strong><br />
the CS-MAS dispersion before film casting on the physicochemical and drug permeability properties <strong>of</strong> the films were<br />
investigated. CS could interact with MAS via electrostatic interaction and intermolecular hydrogen bonding mechanisms,<br />
resulting in nanocomposite formation, for which it was not necessary to apply the heat treatment on the composite<br />
dispersions. The nature <strong>of</strong> the exfoliated and intercalated nanocomposite films formed was depended on the MAS content<br />
added. The heat treatment on the composite dispersions caused an increase in tensile strength, but reduced %elongation <strong>of</strong><br />
the CS-MAS nanocomposite films. The exfoliated nanocomposite films showed higher flexibility, water uptake, and drug<br />
permeability compared to the CS and intercalated CS-MAS nanocomposite films. At higher MAS content, the CS-MAS films<br />
prepared using heat treatment had a lower water uptake, resulting in lower drug permeability when compared with those<br />
prepared using non-heated dispersions. The permeation mechanism <strong>of</strong> non-electrolyte and negatively charged drugs across<br />
the CS-MAS nanocomposite films was predominantly controlled by diffusion in water-filled microchannels, whereas both<br />
adsorption onto MAS and diffusion processes occurred concurrently for the film permeation <strong>of</strong> positively charged drugs. The<br />
findings <strong>of</strong> this study suggest that CS-MAS nanocomposite films can be formed without heating <strong>of</strong> the composite dispersion<br />
before casting. CS-MAS nanocomposites showed strong potential to be used as a film former for coated tablets intended for<br />
modulating drug release. © <strong>2010</strong> Elsevier B.V.<br />
No.548<br />
Author (s) : Kiatboonsri, S., Kiatboonsri, C., Theerawit, P.<br />
Title : Fatal respiratory events caused by zanamivir nebulization<br />
Source : Clinical Infectious Diseases, Volume 50, Issue 4, 15 February <strong>2010</strong>, Page 620<br />
Document Type : Letter<br />
Keywords : dexamethasone, oseltamivir, zanamivir; adult, case<br />
report, coughing, dyspnea, female, fever, filter, human, influenza, Influenza virus A H1N1, letter, medical<br />
nebulizer, nebulization, positive end expiratory pressure, priority journal, ventilator; Adult, Equipment Failure, Fatal<br />
Outcome, Female, Humans, Influenza A Virus, H1N1 Subtype, Influenza, Human, Nebulizers and<br />
Vaporizers, Oxygen, Pregnancy, Pregnancy Complications, Infectious, Ventilators, Mechanical, Zanamivir<br />
<strong>Abstract</strong> : –<br />
No.549<br />
Author (s) : Kiatcharoenpol, T., Punnakitikashem, P., Laosirihongthong, T.<br />
Title : Adoption and assimilation <strong>of</strong> TQM strategy from parent company to overseas plant in Thailand<br />
Source : Proceedings - <strong>2010</strong> IEEE 17th <strong>International</strong> Conference on Industrial Engineering and Engineering Management, IE and EM<strong>2010</strong>,<br />
<strong>2010</strong>, Article number 5645892, Pages 1887-1890<br />
Document Type : Conference Paper<br />
201
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Keywords : Adoption; Assimilation; Resource based-view; Total quality management; TQM<br />
<strong>Abstract</strong> : The purpose <strong>of</strong> this study is to demonstrate how the operation strategy, Total Quality Management (TQM), has been adopted<br />
and assimilated from parent company to oversea plant efficiently and effectively through the use <strong>of</strong> The Resource-Based<br />
View (RBV) theory framework. Insight experiences from a single case study <strong>of</strong> tire cord fabric manufacturing plant locating in<br />
Thailand are revealed. This company is an <strong>of</strong>fshore plant <strong>of</strong> parent company in Turkey. The global strategic foundation <strong>of</strong> this<br />
company consists <strong>of</strong> resources (an employee development aligned with global strategy), values (i.e. integrity, customer focus,<br />
open minded, global collaboration, and results driven), and systems (enabling continual improvement and business<br />
excellence). In order to establish the leading edge in the market, the parent company has been transferring the platform <strong>of</strong><br />
quality and operational excellence, which include TQM strategy, to all manufacturing plants over the world. © <strong>2010</strong> IEEE.<br />
No.550<br />
Author (s) : Kiatchoosakun, S., Keelapang, P., Kaewsuwana, P., Chotinaiwattarakul, C., Piumsomboon, C.<br />
Title : Percutaneous coronary intervention in the elderly: Results from the Thai National Percutaneous Coronary Intervention Registry<br />
(TPCIR)<br />
Source : EuroIntervention, Volume 6, Issue 5, November <strong>2010</strong>, Pages 611-615<br />
Document Type : Article<br />
Keywords : Angioplasty; Coronary artery disease<br />
<strong>Abstract</strong> : Aims: The objective <strong>of</strong> this study was to evaluate the outcomes and identify the risk factors <strong>of</strong> in-hospital mortality among<br />
elderly patients undergoing PCI in Thailand. Methods and results: Included in this study were 4,156 consecutive patients<br />
(comprising 639 elderly [age W75 years] and 3,517 non-elderly [age
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
decrease morbidity including mortality-associated to HIV/AIDS, adverse drug effects lead to interruptions in both HIV and TB<br />
therapy. In addition, an important problem when HAART is initiated in patients with TB is the possibility <strong>of</strong> developing<br />
immune reconstitution inflammatory syndrome (IRIS). A six-month regimen consisting <strong>of</strong> isoniazid, rifampicin, pyrazinamide,<br />
and ethambutal for two months followed by isoniazid and rifampicin for four months is a standard regimen for the treatment<br />
<strong>of</strong> known or presumed drug-susceptible TB disease. The following strategy may minimize the risk <strong>of</strong> IRIS. Patients with CD4<br />
cell counts < 100 cells/mm3, efavirenz-based HAART regimen is recommended and should be initiated as soon as the<br />
patients can tolerate TB treatment. Patients with CD4 cell counts 100-350 cells/mm3, HAART should be started at two<br />
months after TB treatment initiation. HAART should be deferred with closed follow-up <strong>of</strong> CD4 cell counts if patients have CD4<br />
cell counts > 350 cells/mm3.<br />
No.553<br />
Author (s) : Kiertiburanakul, S., Apivanich, S., Muntajit, T., Somsakul, S., Malathum, K.<br />
Title : Epidemiology and risk factors <strong>of</strong> catheter-associated bloodstream infections among intensive care unit patients: An experience<br />
from a tertiary care hospital in Thailand<br />
Source : Journal <strong>of</strong> Hospital Infection, Volume 76, Issue 4, December <strong>2010</strong>, Pages 369-371<br />
Document Type : Letter<br />
Keywords : catheter infection, hemodialysis, hospital admission, human, infection risk, intensive care unit, letter, microbial<br />
contamination, tertiary health care, Thailand; Adult, Bacteremia, Catheter-Related<br />
Infections, Female, Hospitals, Humans, Intensive Care Units, Male, Middle Aged, Risk Factors, Thailand<br />
<strong>Abstract</strong> : –<br />
No.554<br />
Author (s) : Kiertiburanakul, S., Apivanich, S., Muntajit, T., Sukkra, S., Sirinavin, S., Leelaudomlipi, S., Wananukul, W., Satapattayavong, B.,<br />
Malathum, K.<br />
Title : H1N1 2009 influenza among healthcare workers in a tertiary care hospital in Thailand<br />
Source : Journal <strong>of</strong> Hospital Infection, Volume 74, Issue 3, March <strong>2010</strong>, Pages 300-302<br />
Document Type : Letter<br />
Keywords : influenza vaccine, oseltamivir; adult, epidemic, female, health care personnel, high risk population, hospital<br />
hygiene, human, infection control, influenza A (H1N1), Influenza virus A H1N1, information<br />
dissemination, letter, male, nonhuman, patient care planning, RNA virus infection, self care, symptom, tertiary health<br />
care, Thailand, vaccination, virus transmission; Adult, Disease Outbreaks, Female, Health<br />
Personnel, Hospitals, Humans, Incidence, Influenza A Virus, H1N1 Subtype, Influenza, Human, Male, Thailand, Young Adult<br />
<strong>Abstract</strong> : –<br />
No.555<br />
Author (s) : Kiertiburanakul, S., Wiboonchutikul, S., Sukasem, C., Chantratita, W., Sungkanuparph, S.<br />
Title : Using <strong>of</strong> nevirapine is associated with intermediate and reduced response to etravirine among HIV-infected patients who<br />
experienced virologic failure in a resource-limited setting<br />
Source : Journal <strong>of</strong> Clinical Virology, Volume 47, Issue 4, April <strong>2010</strong>, Pages 330-334<br />
Document Type : Article<br />
Keywords : Etravirine; HIV; Nevirapine; Resistance; Resource-limited setting<br />
<strong>Abstract</strong> : Background: Non-nucleoside reverse transcriptase inhibitor (NNRTI)-based regimens have been extensively used for treatment<br />
<strong>of</strong> HIV infection in resource-limited settings. Treatment options after failing an initial regimen are limited because <strong>of</strong> crossresistance<br />
<strong>of</strong> NNRTIs. Objective: To determine the factors associated with reduced response to etravirine among patients with<br />
virological failure. Study design: A retrospective study was conducted. We stratified patients into two groups by the total<br />
weighted scores <strong>of</strong> etravirine-resistance-associated mutations (ETV-RAMs), highest response (score 0-2, N = 123) and<br />
intermediate and reduced response (score ≥2.5, N = 61). Factors associated with a score <strong>of</strong> ≥2.5 were evaluated. Results:<br />
There were 184 patients with mean (SD) age <strong>of</strong> 42 (9) years old and 60% were males. Of all, 68% used NNRTI in the failing<br />
regimen and 51% used stavudine/lamivudine as a backbone. Common ETV-RAMs included Y181C (27%), G190A (17%), and<br />
K101E (10%). Higher proportion <strong>of</strong> K101E, K101P, Y181C, G190S, and M230L were found in patients with a score <strong>of</strong> ≥2.5 (p <<br />
0.05, all). By univariate logistic regression, using protease inhibitor (OR 0.22, 95% CI 0.07-0.67), nevirapine (OR 10.56, 95% CI<br />
4.04-27.74), and efavirenz (OR 2.91, 95% CI 1.01-2.51) in the current regimen were associated with a score <strong>of</strong> ≥2.5. By multiple<br />
203
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
logistic regression, only using nevirapine was associated with a score <strong>of</strong> ≥2.5 (OR 7.61, 95% CI 2.40-24.06). Conclusions: Using<br />
nevirapine in the failing regimen was associated with intermediate and reduced response to ETV. The recommendation <strong>of</strong><br />
using nevirapine as a preferred NNRTI should be re-considered in resource-limited settings where efavirenz is accessible. ©<br />
<strong>2010</strong> Elsevier B.V. All rights reserved.<br />
No.556<br />
Author (s) : Kijsipongse, E., Ngamsuriyaroj, S.<br />
Title : Placing pipeline stages on a Grid: Single path and multipath pipeline execution<br />
Source : Future Generation Computer Systems, Volume 26, Issue 1, January <strong>2010</strong>, Pages 50-62<br />
Document Type : Article<br />
Keywords : Communication cost, Communication overheads, Computation costs, Concurrent execution, Data movements, Grid computing<br />
environment, Grid computing systems, Grid node, Multi-path, Novel methods, Pipeline execution, Placement<br />
methods, Processing Time, Scientific data, Simulated environment, Single path, Spatial parallelism, Temporal parallelism;<br />
Computer science, Computer systems, Data visualization, Grid computing; Pipelines<br />
<strong>Abstract</strong> : In a Grid computing environment, several applications such as scientific data analysis and visualization are naturally<br />
computation and communication intensive. These applications can be decomposed into a sequence <strong>of</strong> pipeline stages which<br />
can be placed on different Grid nodes for concurrent execution. Due to the aggregation <strong>of</strong> the computation and<br />
communication costs involved, finding the way to place such pipeline stages on a Grid in order to achieve the maximum<br />
application throughput becomes a challenging problem. This paper proposes a solution that considers both the pipeline<br />
placement and the data movement between stages. Specifically, we try to minimize the computation cost <strong>of</strong> the pipeline<br />
stages while preventing the communication overhead between successive stages from dominating the entire processing time.<br />
Our proposed solution consists <strong>of</strong> two novel methods. The first method is single path pipeline execution, which exploits only<br />
temporal parallelism, and the second method is multipath pipeline execution, which considers both temporal and spatial<br />
parallelism inherent in any pipeline applications. We evaluate our work in a simulated environment and also conduct a set <strong>of</strong><br />
experiments in a real Grid computing system. When compared with the results from several traditional placement methods,<br />
our proposed methods give the highest throughput. © 2009 Elsevier B.V. All rights reserved.<br />
No.557<br />
Author (s) : Kijvikai, K., Wongampornpat, T., Kongcharoensombat, W.<br />
Title : Laparoscopic donor nephrectomy in Ramathibodi hospital; the largest series in Thailand<br />
Source : INTERNATIONAL JOURNAL OF UROLOGY, Volume: 17, Supplement: 1, Pages: A143-A144, Published: AUG <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.558<br />
Author (s) : Kikuchi, K., Kawahara, K., Biswas, K.K., Ito, T., Tancharoen, S., Shiomi, N., Koda, Y., Matsuda, F., Morimoto, Y., Oyama, Y.,<br />
Takenouchi, K., Miura, N., Arimura, N., Nawa, Y., Arimura, S., Jie, M.X., Shrestha, B., Iwata, M., Mera, K., Sameshima, H., Ohno, Y.,<br />
Maenosono, R., Tajima, Y., Uchikado, H., Kuramoto, T., Nakayama, K., Shigemori, M., Yoshida, Y., Hashiguchi, T., Maruyama, I.<br />
Title : HMGB1: A new marker for estimation <strong>of</strong> the postmortem interval<br />
Source : Experimental and Therapeutic Medicine, Volume 1, Issue 1, January <strong>2010</strong>, Pages 109-111<br />
Document Type : Article<br />
Keywords : Forensic medicine; High mobility group box-1; Postmortem interval<br />
<strong>Abstract</strong> : Estimation <strong>of</strong> the postmortem interval (PMIPMIPMI) is one <strong>of</strong> the most important tasks in forensic medicine. Numerous<br />
methods have been proposed for the determination <strong>of</strong> the time since death by chemical means. High mobility group box-1<br />
(HMHMGB1), a nonhistone DNADNADNA-binding protein is released by eukaryotic cells upon necrosis. Postmortem serum<br />
levels <strong>of</strong> HMGB1 <strong>of</strong> 90 male Wistar rats stored at 4, 14 and 24 °C since death were measured by enzyme-linked<br />
immunosorbent assay. The serum HMHMGB1 level showed a time-dependent increase up to seven days at 4 °C. At 14 °C, the<br />
HMGB1 level peaked at day 3, decreased at day 4, and then plateaued. At 24 °C, the HMHMGB1 level peaked at day 2,<br />
decreased at day 3, and then plateaued. Our findings suggest that HMGB1 is related to the PMIPMIPMI in rats.<br />
No.559<br />
204
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Author (s) : Kikuchi, K., Kawahara, K.-I., Miyagi, N., Uchikado, H., Kuramoto, T., Morimoto, Y., Tancharoen, S., Miura, N., Takenouchi, K.,<br />
Oyama, Y., Shrestha, B., Matsuda, F., Yoshida, Y., Arimura, S., Mera, K., Tada, K.-I., Yoshinaga, N., Maenosono, R., Ohno, Y.,<br />
Hashiguchi, T., Maruyama, I., Shigemori, M.<br />
Title : Edaravone: A new therapeutic approach for the treatment <strong>of</strong> acute stroke<br />
Source : Medical Hypotheses, Volume 75, Issue 6, December <strong>2010</strong>, Pages 583-585<br />
Document Type : Article<br />
Keywords : disufenton sodium, ebselen, norphenazone, tirilazad, tissue plasminogen activator; acute hemorrhagic stroke, acute ischemic<br />
stroke, animal model, article, attenuation, brain edema, brain hemorrhage, brain infarction, brain ischemia, clinical trial, drug<br />
design, drug efficacy, drug inhibition, human, hypothesis, mouse, neurologic disease, neuroprotection, nonhuman, oxidative<br />
stress, rat, stroke, treatment indication, treatment outcome; Animals, Antipyrine, Free Radical<br />
Scavengers, Humans, Neuroprotective Agents, Rats, Stroke, Tissue Plasminogen Activator; Rattus<br />
<strong>Abstract</strong> : Acute stroke, including acute ischemic stroke (AIS) and acute hemorrhagic stroke, (AHS) is a common medical problem with<br />
particular relevance to the demographic changes in industrialized societies. In recent years, treatments for AIS have emerged,<br />
including thrombolysis with tissue plasminogen activator (t-PA). Although t-PA is the most effective currently available<br />
therapy, it is limited by a narrow therapeutic time window and side effects, and only 3% <strong>of</strong> all AIS patients receive<br />
thrombolysis. Edaravone was originally developed as a potent free radical scavenger and, since 2001, has been widely used<br />
to treat AIS in Japan. It was shown that edaravone extended the narrow therapeutic time window <strong>of</strong> t-PA in rats. The<br />
therapeutic time window is very important for the treatment <strong>of</strong> AIS, and early edaravone treatment is more effective. Thus,<br />
more AIS patients might be rescued by administering edaravone with t-PA. Meanwhile, edaravone attenuates AHS-induced<br />
brain edema, neurologic deficits and oxidative injury in rats. Although edaravone treatment is currently only indicated for AIS,<br />
it does <strong>of</strong>fer neuroprotective effects against AHS in rats. Therefore, we hypothesize that early administration <strong>of</strong> edaravone can<br />
rescue AHS patients as well as AIS patients. Taken together, our findings suggest that edaravone should be immediately<br />
administered on suspicion <strong>of</strong> acute stroke, including AIS and AHS. © <strong>2010</strong> Elsevier Ltd.<br />
No.560<br />
Author (s) : Kim, Y.-M., Kim-Lohsoontorn, P., Bae, J.<br />
Title : Effect <strong>of</strong> unsintered gadolinium-doped ceria buffer layer on performance <strong>of</strong> metal-supported solid oxide fuel cells using<br />
unsintered barium strontium cobalt ferrite cathode<br />
Source : Journal <strong>of</strong> Power Sources, Volume 195, Issue 19, 1 October <strong>2010</strong>, Pages 6420-6427<br />
Document Type : Article<br />
Keywords : Barium strontium cobalt ferrite; Buffer layer; Gadolinium-doped ceria; Metal-supported solid oxide fuel cell; Unsintered<br />
buffer layer; Unsintered cathode<br />
<strong>Abstract</strong> : In this study, a Gd0.1Ce0.9O1.95 (GDC) buffer layer and a Ba0.5Sr0.5Co0.8Fe0.2O3-δ (BSCF) cathode, fabricated without presintering,<br />
are investigated (unsintered GDC and unsintered BSCF). The effect <strong>of</strong> the unsintered GDC buffer layer, including the<br />
thickness <strong>of</strong> the layer, on the performance <strong>of</strong> solid oxide fuel cells (SOFCs) using an unsintered BSCF cathode is studied. The<br />
maximum power density <strong>of</strong> the metal-supported SOFC using an unsintered BSCF cathode without a buffer layer is 0.81 W cm-<br />
2, which is measured after 2 h <strong>of</strong> operation (97% H2 and 3% H2O at the anode and ambient air at the cathode), and it<br />
significantly decreases to 0.63 W cm-2 after 50 h. At a relatively low temperature <strong>of</strong> 800 °C, SrZrO3 and BaZrO3, arising from<br />
interaction between BSCF and yttria-stabilized zirconia (YSZ), are detected after 50 h. Introducing a GDC interlayer between<br />
the cathode and electrolyte significantly increases the durability <strong>of</strong> the cell performance, supporting over 1000 h <strong>of</strong> cell<br />
usage with an unsintered GDC buffer layer. Comparable performance is obtained from the anode-supported cell when using<br />
an unsintered BSCF cathode with an unsintered GDC buffer layer (0.75 W cm-2) and sintered GDC buffer layer (0.82 W cm-2).<br />
When a sintered BSCF cathode is used, however, the performance increases to 1.23 W cm-2. The adhesion between the BSCF<br />
cathode and the cell can be enhanced by an unsintered GDC buffer layer, but an increase in the layer thickness (1-6 μm)<br />
increases the area specific resistance (ASR) <strong>of</strong> the cell, and the overly thick buffer layer causes delamination <strong>of</strong> the BSCF<br />
cathode. Finally, the maximum power densities <strong>of</strong> the metal-supported SOFC using an unsintered BSCF cathode and<br />
unsintered GDC buffer layer are 0.78, 0.64, 0.45 and 0.31 W cm-2 at 850, 800, 750 and 700 °C, respectively. © <strong>2010</strong> Elsevier<br />
B.V. All rights reserved.<br />
No.561<br />
Author (s) : Kim, K. K.; Prayoonwiwat, N.; Wicklein, E. M.; Fredrikson, S.; Langdon, D.<br />
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Title : Cognitive performance in patients with early multiple sclerosis from Asia: 2-year data from CogniMS, a worldwide longitudinal<br />
study<br />
Source : MULTIPLE SCLEROSIS, Volume: 16, Issue: 10, Pages: 1275-1276, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.562<br />
Author (s) : Kim, K. K.; Prayoonwiwat, N.; Wicklein, E. M.; Fredrikson, S.; Langdon, D.<br />
Title : Neuropsychological and Health-Related Quality <strong>of</strong> Life Aspects <strong>of</strong> Early Multiple Sclerosis (MS) in Asia: Baseline Data From the<br />
CogniMS Study Asian Cohort<br />
Source : MULTIPLE SCLEROSIS, Volume: 16, Issue: 2, Pages: 267-267, Published: FEB <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.563<br />
Author (s) : Kim-Lohsoontorn, P., Brett, D.J.L., Laosiripojana, N., Kim, Y.-M., Bae, J.-M.<br />
Title : Performance <strong>of</strong> solid oxide electrolysis cells based on composite La0.8Sr0.2MnO3-δ - yttria stabilized zirconia and<br />
Ba0.5Sr0.5Co0.8Fe0.2O3-δ oxygen electrodes<br />
Source : <strong>International</strong> Journal <strong>of</strong> Hydrogen Energy, Volume 35, Issue 9, May <strong>2010</strong>, Pages 3958-3966<br />
Document Type : Article<br />
Keywords : Area-specific resistances, Cobalt ferrites, Electrochemical impedance spectroscopy measurements, Electrochemical<br />
performance, Electrolysers, Electrolysis current, Fuel cell modes, Hydrogen electrodes, Hydrogen generations, Incell,<br />
Lanthanum strontium manganite, Microstructural changes, Microstructure analysis, Operating condition, Operating<br />
temperature, Oxygen electrode, Performance degradation, Positive effects, Presence <strong>of</strong> water, Solid oxide, Solid oxide<br />
electrolysis cells; Barium, Barium compounds, Cells, Cobalt, Cytology, Degradation, Electrochemical corrosion, Electrochemical<br />
impedance spectroscopy, Electrolysis, Electrolytic cells, Ferrites, Fuel cells, Hydrogen production, Lanthanum, Manganese<br />
oxide, Open circuit voltage, Oxide minerals, Oxygen, Remelting, Strontium, Yttria stabilized zirconia, Yttrium alloys, Zirconia;<br />
Electrochemical electrodes<br />
<strong>Abstract</strong> : The electrochemical performance <strong>of</strong> solid oxide electrolysis cells (SOECs) having barium strontium cobalt ferrite<br />
(Ba0.5Sr0.5Co0.8Fe0.2O3-δ) and composite lanthanum strontium manganite-yttria stabilized zirconia (La0.8Sr0.2MnO3-δ-YSZ)<br />
oxygen electrodes has been studied over a range <strong>of</strong> operating conditions. Increasing the operating temperature (973 K to 1173<br />
K) significantly increased electrochemical performance and hydrogen generation efficiency for both systems. The presence <strong>of</strong><br />
water in the hydrogen electrode was found to have a marked positive effect on the EIS response <strong>of</strong> solid oxide cell (SOC)<br />
under open circuit voltage (OCV). The difference in operation between electrolytic and galvanic modes was investigated. Cells<br />
having BSCF oxygen electrodes (Ni-YSZ/YSZ/BSCF) showed greater performance than LSM-YSZ-based cells (Ni-YSZ/YSZ/LSM-<br />
YSZ) over the range <strong>of</strong> temperatures, in both galvanic and electrolytic regimes <strong>of</strong> operation. The area specific resistance (ASR)<br />
<strong>of</strong> the LSM-YSZ-based cells remained unchanged when transitioning between electrolyser and fuel cell modes; however, the<br />
BSCF cells exhibited an overall increase in cell ASR <strong>of</strong> ∼2.5 times when entering electrolysis mode. Durability studies <strong>of</strong> cells<br />
in electrolysis mode were made over 20 h periods. Significant degradation <strong>of</strong> the BSCF cell was observed (0.02 V h-1) while<br />
the LSM-YSZ cell exhibited more stable performance under the same operating conditions (0.3 A cm-2, 1123 K, and H2O/H2 =<br />
70/30). Increasing the electrolysis current density accelerated performance degradation. Electrochemical impedance<br />
spectroscopy measurements and microstructure analysis were used to investigate the cause <strong>of</strong> performance degradation, with<br />
evidence emerging <strong>of</strong> microstructural change in the case <strong>of</strong> the BSCF electrode. © <strong>2010</strong> Pr<strong>of</strong>essor T. Nejat Veziroglu.<br />
No.564<br />
Author (s) : Kimpakorn, N., Tocquer, G.<br />
Title : Service brand equity and employee brand commitment<br />
Source : Journal <strong>of</strong> Services Marketing, Volume 24, Issue 5, <strong>2010</strong>, Pages 378-388<br />
Document Type : Article<br />
Keywords : Brand equity; Brand loyalty; Brands; Employees<br />
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<strong>Abstract</strong> : Purpose: The aims <strong>of</strong> this article are to measure the brand equity <strong>of</strong> service firms (luxury hotels) using a customer<br />
perspective, to identify factors that predict customers' brands relationships and to explore the links between service brand<br />
equity and employee brand commitment Design/methodology/approach: Two surveys were conducted to achieve the<br />
research objectives. The first survey objective was to measure brand equity using a sample <strong>of</strong> 250 international customers <strong>of</strong><br />
five-star hotels in Bangkok. The second survey objective was to collect information regarding employee brand commitment in<br />
each selected hotel using a sample <strong>of</strong> 250 employees. Findings: Results show that hotels belonging to the same category<br />
have different brand equity and that brand differentiation and brand trust are the variables that have the major influence on<br />
customer brand relationships. Perceived service quality and associations related to hotel core services are not related to<br />
brand relationships. High hotel brands equity have a stronger level <strong>of</strong> employee brand commitment that low hotel brands<br />
equity. Research limitations/implications: For hotel managers the research findings illustrate the importance <strong>of</strong> brand<br />
differentiation and trust for international hotels chains and illustrate the importance <strong>of</strong> employee brand commitment in the<br />
process <strong>of</strong> building a strong band. Therefore this research has an implication not only for marketing but also for human<br />
resource managers and for hotel general managers. Originality/value: The value <strong>of</strong> this research resides in the exploration<br />
between service brand equity and employee brand commitment. The literature on service marketing emphasizes the link<br />
between employee and service quality but to the authors' knowledge little research has explored the link between the<br />
service brand and its employees. © Emerald Group Publishing Limited.<br />
No.565<br />
Author (s) : King, B., Yong, H.-H., Borland, R., Omar, M., Ahmad, A.A., Sirirassamee, B., Hamann, S., O'Connor, R.J., Bansal-Travers, M., Elton-<br />
Marshall, T., Lee, W.B., Hammond, D., Thrasher, J.<br />
Title : Malaysian and Thai smokers' beliefs about the harmfulness <strong>of</strong> 'light' and menthol cigarettes<br />
Source : Tobacco Control, Volume 19, Issue 6, December <strong>2010</strong>, Pages 444-450<br />
Document Type : Article<br />
Keywords : menthol, nicotine, tar; adolescent, adult, article, attitude to health, cultural anthropology, female, human, information<br />
processing, Malaysia, male, middle aged, smoking, Thailand, tobacco; Adolescent, Adult, Culture, Data Collection, Female,<br />
Health Knowledge, Attitudes, Practice, Humans, Malaysia, Male, Menthol, Middle Aged, Nicotine, Smoking, Tars, Thailand,<br />
Tobacco, Young Adult<br />
<strong>Abstract</strong> : Objective: This study explored the extent to which Malaysian and Thai smokers believe "light" and menthol cigarettes are less<br />
harmful than "regular" cigarettes and the correlates <strong>of</strong> these beliefs. Methods: The study used data from wave 1 <strong>of</strong> the<br />
<strong>International</strong> Tobacco Control Southeast Asia Survey. 2006 adult smokers (95.3% male) from Malaysia and 2000 adult smokers<br />
(94.5% male) from Thailand were interviewed face to face in 2005. Results: 29% <strong>of</strong> Malaysian respondents reported currently<br />
smoking light cigarettes and 14% menthols, with 19% agreeing that lights are less harmful and 16% agreeing that menthols<br />
are less harmful. 38% <strong>of</strong> Thai respondents reported currently smoking light cigarettes and 19% menthols, with 46% agreeing<br />
that lights are less harmful and 35% agreeing that menthols are less harmful. Malaysian smokers reporting current use <strong>of</strong> light<br />
or menthol cigarettes were more likely to believe that they are less harmful. Reported use <strong>of</strong> lights did not relate to beliefs<br />
for Thai respondents. The belief that light and/or menthol cigarettes are less harmful was strongly related to the belief that<br />
they have smoother smoke. Conclusions: The experience <strong>of</strong> smoother smoke is likely to produce some level <strong>of</strong> belief in<br />
reduced harm, regardless <strong>of</strong> how brands are labelled and whether or not Federal Trade Commission FTC/<strong>International</strong><br />
Organisation for Standardisation tar, nicotine and carbon monoxide yield figures are used. Copyright Article author (or their<br />
employer) <strong>2010</strong>.<br />
No.566<br />
Author (s) : Kiratisin, P., Apisarnthanarak, A., Kaewdaeng, S.<br />
Title : Synergistic activities between carbapenems and other antimicrobial agents against Acinetobacter baumannii including<br />
multidrug-resistant and extensively drug-resistant isolates<br />
Source : <strong>International</strong> Journal <strong>of</strong> Antimicrobial Agents, Volume 36, Issue 3, September <strong>2010</strong>, Pages 243-246<br />
Document Type : Article<br />
Keywords : Acinetobacter baumannii; Carbapenems; Combination therapy; Synergy<br />
<strong>Abstract</strong> : Treatment options for multidrug-resistant (MDR) and extensively drug-resistant (XDR) Acinetobacter baumannii have been<br />
seriously limited and may require combination antimicrobial therapy. In this study, we searched for synergistic activity<br />
between carbapenems (doripenem, imipenem and meropenem) and various non-traditional agents (cefoperazone/sulbactam,<br />
doxycycline, rifampicin, netilmicin and moxifloxacin) against 40 A. baumannii clinical isolates, including MDR and XDR isolates.<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
The results showed that combination <strong>of</strong> each carbapenem with cefoperazone/sulbactam, based on the Etest method,<br />
demonstrated synergy more frequently (17.5-32.5%) than the other tested agents, which may suggest a role in combination<br />
therapy against highly resistant A. baumannii. © <strong>2010</strong> Elsevier B.V. and the <strong>International</strong> Society <strong>of</strong> Chemotherapy.<br />
No.567<br />
Author (s) : Kiratisin, P., Henprasert, A.<br />
Title : Genotypic analysis <strong>of</strong> plasmid-mediated β-lactamases amongst Enterobacteriaceae other than Escherichia spp. and Klebsiella<br />
spp. that are non-susceptible to a broad-spectrum cephalosporin<br />
Source : <strong>International</strong> Journal <strong>of</strong> Antimicrobial Agents, Volume 36, Issue 4, October <strong>2010</strong>, Pages 343-347<br />
Document Type : Article<br />
Keywords : β-Lactamase; AmpC; Carbapenemase; Cephalosporins; Enterobacteriaceae; ESBL<br />
<strong>Abstract</strong> : Enterobacteriaceae other than Escherichia spp. and Klebsiella spp. are recognisable for their increasing resistance. Plasmidmediated<br />
β-lactamases, including extended-spectrum β-lactamases (ESBLs), plasmid-mediated AmpC β-lactamases<br />
(pAmpCs) and the newly emerged KPC, play an important role in β-lactam resistance. In this study, we investigated the<br />
genetic characteristics <strong>of</strong> plasmid-mediated β-lactamases amongst non-Escherichia, non-Klebsiella Enterobacteriaceae that<br />
were non-susceptible to at least a broad-spectrum cephalosporin. A total <strong>of</strong> 143 (23.9%) <strong>of</strong> 598 isolates during the 6-month<br />
study period met the screening criteria, amongst which 142 (99.3%) and 99 (69.2%) isolates carried ESBL and pAmpC genes,<br />
respectively. Resistance genotypes were described. blaKPC was not detected in isolates with reduced susceptibility to<br />
carbapenems. This study provides an insight into plasmid-mediated resistance determinants amongst cephalosporin-nonsusceptible<br />
Enterobacteriaceae other than Escherichia spp. and Klebsiella spp. © <strong>2010</strong> Elsevier B.V. and the <strong>International</strong><br />
Society <strong>of</strong> Chemotherapy.<br />
No.568<br />
Author (s) : Kirawanich, P., Islam, N.E.<br />
Title : Developments in mung bean (Vigna radiata L.) and water convolvulus (Ipomoea aquatica Forssk.) yields by narrow-band<br />
nonionizing radiations<br />
Source : IEEE Transactions on Plasma Science, Volume 38, Issue 8 PART 2, August <strong>2010</strong>, Article number 5419118, Pages 1942-1948<br />
Document Type : Conference Paper<br />
Keywords : Agricultural applications; electromagnetic (EM) exposure chamber; nonionizing-radiation (NIR) treatment<br />
<strong>Abstract</strong> : A 50-Ω exposure chamber was constructed to provide a uniform electromagnetic (EM) field exposure environment for<br />
observations on the yields <strong>of</strong> mung beans ( Vigna radiata L.) and water convolvuluses ( Ipomoea aquatica Forssk.). The growth<br />
performances were compared at two radio frequencies (300 and 425 MHz) through several combinations <strong>of</strong> field strengths (1<br />
and 100 mW) and exposure durations (1 and 2 h). Among both frequencies, the superior enhancements in average seedling<br />
length <strong>of</strong> both seed varieties were observed at 425 MHz. In addition, statistical analyses <strong>of</strong> the results show that at 300 MHz,<br />
both seed varieties required an exposure period <strong>of</strong> 2 h to result in the growth improvement, while at 425 MHz, mung beans<br />
demanded less exposure duration (1 h) than did water convolvuluses (2 h) to see the development, and this could be<br />
explained through the specific absorption rates <strong>of</strong> EM fields inside the area under test. Among exposure cases, the highest<br />
growth development <strong>of</strong> 48.5% (P < 0.01) was observed in mung bean treatment when the exposure combination was 100<br />
mW, 1 h, and 425 MHz. © <strong>2010</strong> IEEE.<br />
No.569<br />
Author (s) : Kirawanich, P., Pausawasdi, N., Srisawat, C., Wilairat, D., Neatpisarnvanit, C.<br />
Title : Numerical schemes <strong>of</strong> nanosecond pulsed electric field systems for cell apoptosis applications<br />
Source : ECTI-CON <strong>2010</strong> - The <strong>2010</strong> ECTI <strong>International</strong> Conference on Electrical Engineering/Electronics, Computer,<br />
Telecommunications and Information Technology, <strong>2010</strong>, Article number 5491543, Pages 6-10<br />
Document Type : Conference Paper<br />
Keywords : Finite-difference time-domain method; Nanosecond pulsed electric field; Transmembrane potential<br />
<strong>Abstract</strong> : Characteristics <strong>of</strong> a 1 J/cm3 nanosecond pulsed electric field (nsPEF) system and cells under exposures are reported here<br />
through a finite-difference time-domain (FDTD) analysis. The system is composed <strong>of</strong> a pulse forming line (PFL) and a universal<br />
electroporation cuvette filled with a buffer solution. The system is primarily designed via PSPICE model. The interactions<br />
between the PFL and the cuvette are taken into account by one-dimensional Telegrapher's equations for the PFL and threedimensional<br />
Maxwell's curl equations for the cuvette volume. The voltage across the cuvette electrodes are validated<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
through the laboratory-constructed nsPEF system. The observed electrically invisible effect <strong>of</strong> cells from transmembrane<br />
potentials across plasma and organelle membranes in both time and frequency domains increases the probability <strong>of</strong> electric<br />
field interactions with intracellular structures.<br />
No.570<br />
Author (s) : Kirawanich, P., Pausawasdi, N., Srisawat, C., Yakura, S.J., Islam, N.E.<br />
Title : An FDTD interaction scheme <strong>of</strong> a high-intensity nanosecond-pulsed electric-field system for in vitro cell apoptosis applications<br />
Source : IEEE Transactions on Plasma Science, Volume 38, Issue 10 PART 1, October <strong>2010</strong>, Article number 5404942, Pages 2574-2582<br />
Document Type : Conference Paper<br />
Keywords : Finite-difference time-domain (FDTD) method; nanosecond-pulsed electric field (nsPEF); pulse-forming line (PFL)<br />
<strong>Abstract</strong> : A finite-difference time-domain analysis <strong>of</strong> a high-intensity nanosecond-pulsed electric-field (nsPEF) system, composed <strong>of</strong> a<br />
pulse-forming line (PFL) and a universal electroporation cuvette, is described. The simulation scheme is based on interactions<br />
<strong>of</strong> 1-D transmission-line equations for the PFL and 3-D Maxwell's curl equations for the cuvette volume. Simulations<br />
incorporate system adjustment to facilitate maximum transfer <strong>of</strong> electrical energy from the PFL to the cuvette medium.<br />
Experimental validation <strong>of</strong> the voltage across the cuvette electrodes through the laboratory-constructed nsPEF system with<br />
an energy density <strong>of</strong> ∼1 J/cm3 reveals an overall agreement with some discrepancies. The distribution pr<strong>of</strong>iles <strong>of</strong> the<br />
transient field inside the cell suspension area during the excitation <strong>of</strong> 5-kV 10-ns pulses would adequately account for the<br />
feasibility <strong>of</strong> using an integrated model as a design benchmark for the interaction physics <strong>of</strong> the generated nanosecond pulses<br />
and culture vessel. The observed nsPEF effects on cells include increased transmembrane potentials across organelle<br />
membranes without permanently damaging the cell membrane, increasing the probability <strong>of</strong> electric field interactions with<br />
intracellular structures. © <strong>2010</strong> IEEE.<br />
No.571<br />
Author (s) : Kiriwat, O., Petyim, S.<br />
Title : The effects <strong>of</strong> transdermal contraception on lipid pr<strong>of</strong>iles, carbohydrate metabolism and coagulogram in Thai women<br />
Source : Gynecological Endocrinology, Volume 26, Issue 5, May <strong>2010</strong>, Pages 361-365<br />
Document Type : Article<br />
Keywords : Carbohydrate metabolism; Coagulogram; Contraceptive patch; Lipid pr<strong>of</strong>ile<br />
<strong>Abstract</strong> : Objective.To evaluate the effects <strong>of</strong> the contraceptive patch on lipid pr<strong>of</strong>iles, carbohydrate metabolism and coagulogram in<br />
Thai women. Methods.Fifty healthy Thai women were assigned to use contraceptive patches. Blood chemistries test including<br />
liver function test, lipid pr<strong>of</strong>iles and coagulogram were evaluated at baseline, cycles 3 and 6. Results.Total cholesterol,<br />
triglyceride and HDL were significantly increased, whereas LDL was slightly decreased. The ratio <strong>of</strong> total cholesterol/HDL and<br />
LDL/HDL significantly decreased when applying the patch. After discontinued use <strong>of</strong> contraceptive patch, the women whose<br />
blood tests present hypercholesterol during patch use showed a continuous decrease in blood results <strong>of</strong> total cholesterol<br />
level over 3 months. Moreover, mean fasting glucose, SGOT, SGPT and alkaline phosphate were decreased. No woman<br />
suffering from VTE in this study. Conclusions.The use <strong>of</strong> contraceptive patch does not exert a negative effect on carbohydrate<br />
metabolism, lipid pr<strong>of</strong>ile, liver function test and blood coagulogram. However, further studies are required to elucidate the<br />
effect <strong>of</strong> the contraceptive on the patch user in long term. © <strong>2010</strong> Informa UK Ltd.<br />
No.572<br />
Author (s) : Kirk, P., He, T., Anderson, L.J., Roughton, M., Tanner, M.A., Lam, W.W.M., Au, W.Y., Chu, W.C.W., Chan, G., Galanello, R., Matta,<br />
G., Fogel, M., Cohen, A.R., Tan, R.S., Chen, K., Ng, I., Lai, A., Fucharoen, S., Laothamata, J., Chuncharunee, S., Jongjirasiri, S.,<br />
Firmin, D.N., Smith, G.C., Pennell, D.J.<br />
Title : <strong>International</strong> reproducibility <strong>of</strong> single breathhold T2* MR for cardiac and liver iron assessment among five thalassemia centers<br />
Source : Journal <strong>of</strong> Magnetic Resonance Imaging, Volume 32, Issue 2, August <strong>2010</strong>, Pages 315-319<br />
Document Type : Article<br />
Keywords : Cardiac siderosis; Cardiomyopathy; Cardiovascular MR; Thalassemia<br />
<strong>Abstract</strong> : Purpose: To examine the reproducibility <strong>of</strong> the single breathhold T2* technique fromdifferent scanners, after installation <strong>of</strong><br />
standardmethodology in five international centers. Materials and Methods: Up to 10 patients from each center were scanned<br />
twice locally for local interstudy reproducibility <strong>of</strong> heart and liver T2*, and then flown to a central MR facility to be rescanned<br />
on a reference scanner for intercenter reproducibility. Interobserver reproducibility for all scans was also assessed. Results: Of<br />
the 49 patients scanned, the intercenter reproducibility for T2* was 5.9% for the heart and 5.8% for the liver. Local interstudy<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
reproducibility for T2* was 7.4% for the heart and 4.6% for the liver. Interobserver reproducibility for T2* was 5.4% for the<br />
heart and 4.4% for the liver. Conclusion: These data indicate that T2* MR may be developed into a widespread test for tissue<br />
siderosis providing that well-defined and approved imaging and analysis techniques are used. © <strong>2010</strong> Wiley-Liss, Inc.<br />
No.573<br />
Author (s) : Kitamura, S., Thong-Aree, S., Madsri, S., Poonswad, P.<br />
Title : Mammal diversity and conservation in a small isolated forest <strong>of</strong> southern Thailand<br />
Source : Raffles Bulletin <strong>of</strong> Zoology, Volume 58, Issue 1, February <strong>2010</strong>, Pages 145-156<br />
Document Type : Article<br />
Keywords : Camera trapping; Dipterocarp forest; Hala-bala wildlife sanctuary; Rarefaction curve<br />
<strong>Abstract</strong> : Knowledge <strong>of</strong> the presence and distribution <strong>of</strong> species is crucial for designing and evaluating conservation strategies within a<br />
region. We conducted a camera-trapping survey <strong>of</strong> terrestrial mammal and bird diversity in a small isolated forest <strong>of</strong> southern<br />
Thailand over 3 yr. A total <strong>of</strong> 15 camera traps, which accumulated 11,106 camera-days, were set in three forest types:<br />
primary, logged, and hill forests. Despite its small size, isolation, and surrounding agricultural areas, a total <strong>of</strong> 35 mammal<br />
species, eight bird species, and one reptile species were recorded in the forest system. The total number <strong>of</strong> species<br />
photographed was similar among forest types (26-30 species), and rarefaction curves <strong>of</strong> each forest did not indicate any<br />
differences in the relationship between sampling effort and recorded species richness. As the activity period <strong>of</strong> these animals<br />
does not appear to be affected by human activities, we believe that the effect <strong>of</strong> direct poaching on large mammals in the<br />
study area is negligible. Although we did not observe any previously unrecorded animals in our study site, our findings are<br />
very valuable and point to the importance <strong>of</strong> biodiversity conservation efforts in these small fragmented and humanmodified<br />
forest landscapes. © National <strong>University</strong> <strong>of</strong> Singapore.<br />
No.574<br />
Author (s) : Kitani, S., Doi, M., Shimizu, T., Maeda, A., Nihira, T.<br />
Title : Control <strong>of</strong> secondary metabolism by farX, which is involved in the γ-butyrolactone biosynthesis <strong>of</strong> Streptomyces lavendulae<br />
FRI-5<br />
Source : Archives <strong>of</strong> Microbiology, Volume 192, Issue 3, March <strong>2010</strong>, Pages 211-220<br />
Document Type : Article<br />
Keywords : D-cycloserine; Diffusing signaling molecule; Negative autoregulatory circuit; Signal transduction<br />
<strong>Abstract</strong> : The γ-butyrolactone signaling system is distributed widely among streptomycetes as an important regulatory mechanism <strong>of</strong><br />
antibiotic production and/or morphological differentiation. IM-2 [(2R,3R,1′R)-2-(1′- hydroxybutyl)-3- hydroxymethyl-γbutanolide]<br />
is a γ-butyrolactone that switches <strong>of</strong>f the production <strong>of</strong> D-cycloserine but switches on the production <strong>of</strong> several<br />
nucleoside antibiotics as well as blue pigment in Streptomyces lavendulae FRI-5. farX is a member <strong>of</strong> the afsA-family genes,<br />
which are proposed to encode enzymes involved in γ-butyrolactone biosynthesis. Disruption <strong>of</strong> farX caused overproduction<br />
<strong>of</strong> D-cycloserine, and abolished production <strong>of</strong> nucleoside antibiotic and blue pigment with the loss <strong>of</strong> IM-2 production. The<br />
Wnding that all phenotypic changes observed in the farX disruptant were restored by the addition <strong>of</strong> exogenous IM-2<br />
suggested that FarX plays a biosynthetic role in IM-2 production. Transcriptional comparison between the wild-type strain and<br />
the farX disruptant revealed that, in addition to already known genes farR1 and farR2, several other genes (farR4, farD, and<br />
farE) are under the transcriptional regulation <strong>of</strong> IM-2. Furthermore, the fact that farX transcription is under the control <strong>of</strong> IM-2<br />
suggested that S. lavendulae FRI-5 has a fine-tuning system to control γ-butyrolactone production. © Springer-Verlag <strong>2010</strong>.<br />
No.575<br />
Author (s) : Kitikomonkun, P.; Charatcharoenwitthaya, P.; Chainuwatti, S.; Nimanong, S.; Pausawasdi, N.; Prachayakul, V.; Pongprasobchai, S.;<br />
Udompanthurak, S.; Leelakusolvong, S.; Manatsathit, S.; Kachintorn, U.; Tanwandee, T.<br />
Title : Use <strong>of</strong> HBsAg level alone or combined with HBV DNA can better separate truly inactive chronic hepatitis B infection from<br />
those with subsequent reactivation<br />
Source : JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY, Volume: 25, Supplement: 2, Pages: A93-A94, Published: SEP <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.576<br />
Author (s) : Kitiyanant, N.; Svendsen, C. N.; Kitiyanant, Y.; Thangnipon, W.<br />
Title : TROPHIC FACTORS RESCUE CHOLINERGIC FUNCTIONS FROM AMYLOID-beta-INDUCED NEUROTOXICITY IN CULTURED SEPTAL<br />
NEURONS<br />
Source : JOURNAL OF NEUROCHEMISTRY, Volume: 115, Supplement: 1, Pages: 42-42, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.577<br />
Author (s) : Kitkumthorn, N., Mutirangura, A.<br />
Title : LINE-1 methylation difference between ameloblastoma and keratocystic odontogenic tumor<br />
Source : Oral Diseases, Volume 16, Issue 3, April <strong>2010</strong>, Pages 286-291<br />
Document Type : Article<br />
Keywords : Ameloblastoma; COBRALINE-1; Keratocystic odontogenic tumor; LINE-1; Methylation level<br />
<strong>Abstract</strong> : Objective: Global hypomethylation is a common epigenetic event in cancer. Keratocystic odontogenic tumor (KCOT) and<br />
ameloblastoma are different tumors but posses the same tissue in origin. Here, we investigated long interspersed nuclear<br />
element-1 (LINE-1 or L1) methylation status between ameloblastoma and KCOT. Materials and methods: We studied the<br />
methylation levels <strong>of</strong> the long interspersed nucleotide element-1 (LINE-1) in ameloblastoma and KCOT. After collecting<br />
ameloblastoma cells and epithelium lining cells <strong>of</strong> KCOT by laser capture microdissection from paraffin embedded tissue,<br />
combined bisulfite restriction analysis <strong>of</strong> LINE-1 (COBRALINE-1) was performed to measure LINE-1 methylation levels. Results:<br />
The LINE-1 methylation level in KCOT (53.16 ± 12.03%) was higher than that in ameloblastoma (36.90 ± 16.52%), with a<br />
statistical significance <strong>of</strong> P = 0.001. The ranges <strong>of</strong> LINE-1 methylation <strong>of</strong> both lesions were not associated with either age or<br />
sex. Conclusion: We found LINE-1 hypomethylation levels between ameloblastoma and KCOT are different. Therefore, global<br />
methylations between these tumors are processed differently. © <strong>2010</strong> John Wiley & Sons A/S.<br />
No.578<br />
Author (s) : Kitkumthorn, N.; Yanatatsaneejit, P.; Rabalert, J.; Dhammawipark, C.; Mutirangura, A.<br />
Title : Association <strong>of</strong> P53 codon 72 polymorphism and ameloblastoma<br />
Source : ORAL DISEASES, Volume: 16, Issue: 7, Pages: 631-635, DOI: 10.1111/j.1601-0825.<strong>2010</strong>.01664.x, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.579<br />
Author (s) : Kitthawee, S., Dujardin, J.-P.<br />
Title : The geometric approach to explore the Bactrocera tau complex (Diptera: Tephritidae) in Thailand<br />
Source : Zoology, Volume 113, Issue 4, August <strong>2010</strong>, Pages 243-249<br />
Document Type : Article<br />
Keywords : Bactrocera tau complex; Fruit fly; Host plants; Morphometrics; Species identification<br />
<strong>Abstract</strong> : Specimens <strong>of</strong> the genus Bactrocera were collected from several host plants in northern and western Thailand. They were<br />
morphologically recognized as Bactrocera tau and were subdivided into eleven samples according to host plant, geographic<br />
origin and time <strong>of</strong> collection. Twelve landmarks <strong>of</strong> the right wing were described in a total <strong>of</strong> 264 males and 276 females. An<br />
exploratory analysis using kernel density estimates was performed on the multivariate morphometric space. Non-parametric<br />
classification highlighted the existence <strong>of</strong> two non-overlapping clusters within both males and females. The clusters were not<br />
congruent with geography. One cluster (cluster I) contained only one plant, Momordica cochinchinensis, the other one<br />
(cluster II) contained five different plants including M. cochinchinensis. Further morphometric analyses on selected samples<br />
indicated that the influence <strong>of</strong> the plants on the shape <strong>of</strong> the wing could not explain satisfactorily the presence <strong>of</strong> two<br />
clusters. Genetic techniques identified the presence <strong>of</strong> B. tau cryptic species C in M. cochinchinensis from cluster I, and <strong>of</strong> B.<br />
tau cryptic species A in Coccinia grandis from cluster II. Our working hypothesis is that the two clusters identified by geometric<br />
morphometrics were species A and C, respectively. © <strong>2010</strong> Elsevier GmbH.<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.580<br />
Author (s) : Kittichanun, C., Phivthong-ngam, L., Niwattisaiwong, N., Piyachaturawat, P., Suksamran, A., Apipalakul, K., Lawanprasert, S.<br />
Title : Effects <strong>of</strong> Curcuma comosa extracts on hepatic cytochrome P450 activities in rats<br />
Source : Journal <strong>of</strong> Health Research, Volume 24, Issue 1, January <strong>2010</strong>, Pages 1-6<br />
Document Type : Article<br />
Keywords : Curcuma comosa; Cytochrome P450; Drug interaction<br />
<strong>Abstract</strong> : Curcuma comosa Roxb. (Zingiberaceae) is an indigenous plant <strong>of</strong> Thailand. The rhizome <strong>of</strong> this plant has been widely used in<br />
Thai traditional medicine for treatment <strong>of</strong> abnormal uterine symptoms. The purpose <strong>of</strong> this study was to investigate effects <strong>of</strong><br />
C. comosa hexane extract and ethanolic extract on hepatic cytochrome P450 (CYP) in rats. Fifty male Wistar rats were<br />
randomly divided into 5 groups (10 rats/group). Rats were given orally with C. comosa hexane extract or ethanolic extract (250<br />
and 500 mg/kg/day) or corn oil in a control group for 30 consecutive days. At the end <strong>of</strong> the treatment, rats were sacrificed<br />
and liver microsomes were prepared. Hepatic microsomal total CYP content and CYP activity were determined. The results<br />
showed that both C. comosa hexane and ethanolic extracts at 250 mg/kg/day caused a significant increase <strong>of</strong> total CYP<br />
contents and the activity <strong>of</strong> CYP1A1. Lower dose <strong>of</strong> both extracts (250 mg/kg/day) caused a more increase <strong>of</strong> CYP1A1 activity<br />
than the higher dose (500 mg/kg/day). Also, both C. comosa hexane and ethanolic extracts caused a dose-dependent<br />
increase <strong>of</strong> CYP2B1/2B2 activities and the increase was higher in the hexane extract group than the ethanolic extract group.<br />
Hexane and ethanolic extracts <strong>of</strong> C. comosa did not affect CYP1A2, CYP2E1 and CYP3A activities. These results indicated the<br />
possibilities <strong>of</strong> C. comosa hexane and ethanolic extracts regarding herb-drug interactions and the increase risk <strong>of</strong> toxicity,<br />
mutagenesis and/or carcinogenesis from drugs or compounds that are metabolized or bioactivated via CYP1A1 and<br />
CYP2B1/2B2.<br />
No.581<br />
Author (s) : Kittigul, L., Pombubpa, K., Taweekate, Y., Diraphat, P., Sujirarat, D., Khamrin, P., Ushijima, H.<br />
Title : Norovirus GII-4 2006b variant circulating in patients with acute gastroenteritis in thailand during a 2006-2007 study<br />
Source : Journal <strong>of</strong> Medical Virology, Volume 82, Issue 5, May <strong>2010</strong>, Pages 854-860<br />
Document Type : Article<br />
Keywords : Acute gastroenteritis; Genotype; Norovirus; RT-nested PCR; Thailand<br />
<strong>Abstract</strong> : Noroviruses (NoVs) are recognized as a significant cause <strong>of</strong> acute gastroenteritis in children and adults. A 14-month study, from<br />
January 2006 to February 2007, was undertaken in a hospital in Thailand to determine the prevalence and genetic<br />
characterization <strong>of</strong> NoVs in patients <strong>of</strong> all ages with acute gastroenteritis. Based on reverse transcription-nested polymerase<br />
chain reaction (RT-nested PCR), NoVs were detected in 122 <strong>of</strong> 273 (44.7%) collected stool samples. Of the 122 NoV-positive<br />
samples, 28 (23%) belonged to GI, 79 (64.8%) belonged to GII, and 15 (12.2%) were mixed infections <strong>of</strong> GI and GII strains.<br />
Three NoV GI-positive and 42 NoV GII-positive samples were characterized successfully by DNA sequencing <strong>of</strong> the RT-nested<br />
PCR products and phylogenetic analysis. For NoV GI, two genotypes were identified: GI-2 (one sample) and GI-6 (two samples).<br />
NoV GII could be classified further into five distinct genotypes: GII-2 (1 sample), GII-3 (3 samples), GII-4 (14 samples), GII-6 (3<br />
samples), and GII-17 (2 samples), and one unclassified genotype (19 samples). All NoV GII-4 strains showed 88-98% nucleotide<br />
identity with NoV GII-4 2006b variants reported worldwide. Among genotypes <strong>of</strong> NoV characterized, one co-infected stool<br />
sample exhibited NoVs GI-6 and GII-4 2006b. This study suggests that there is an important role <strong>of</strong> NoVs as etiologic agents in<br />
patients with acute gastroenteritis. The predominant circulating genotype <strong>of</strong> NoV infections is GII-4 2006b variant. © <strong>2010</strong><br />
Wiley-Liss, Inc.<br />
No.582<br />
Author (s) : Kittigul, L., Pombubpa, K., Sukonthalux, S., Rattanatham, T., Utrarachkij, F., Diraphat, P.<br />
Title : Detection <strong>of</strong> hepatitis a virus and bacterial contamination in raw oysters in Thailand<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 1, January <strong>2010</strong>, Pages 105-113<br />
Document Type : Article<br />
Keywords : Hepatitis A virus; MPN; Oysters; RT-nested PCR; Virus concentration<br />
<strong>Abstract</strong> : This study was conducted to determine the presence <strong>of</strong> hepatitis A virus (HAV) in raw oysters (Crassostrea belcheri) using a<br />
virus concentration method and reverse transcription-nested polymerase chain reaction (RT-nested PCR). A total <strong>of</strong> 220 oyster<br />
samples were collected from oyster farms and local markets in Thailand. HAV was found in three oyster samples. Nested PCR<br />
products <strong>of</strong> HAV detected in oysters were characterized further by DNA sequencing <strong>of</strong> the VP1/2A region and subjected to<br />
phylogenetic analysis. All HAV sequences (168 basepairs) were associated with human HAV subgenotype IB (GIB). Fecal<br />
212
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
coliforms and Escherichia coli were determined using the multiple tube fermentation method, to assess the microbiological<br />
quality <strong>of</strong> collected oysters. Among oyster samples tested, 65% had fecal coliforms higher than the standard level for raw<br />
shellfish [<20 Most Probable Numbers (MPN)/g]; MPN values in the range <strong>of</strong> 21.0-4.6 x 104/g. Most oyster samples (85%)<br />
were contaminated with E. coli in the range <strong>of</strong> 3.0-4.6 x 104MPN/g. One oyster sample with an acceptable level <strong>of</strong> fecal<br />
coliforms contained HAV GIB. E. coli was found in all HAV-positive oyster samples. The results suggest a significant presence <strong>of</strong><br />
HAV and bacterial indicators <strong>of</strong> fecal contamination in raw oysters, which are a health risk for consumers and a source <strong>of</strong><br />
gastrointestinal illness. Enteric viruses should also be tested to assess the microbiological quality <strong>of</strong> oysters.<br />
No.583<br />
Author (s) : Kladsomboon, S., Pratontep, S., Kerdcharoen, T.<br />
Title : Optical electronic nose based on porphyrin and phthalocyanine thin films<br />
Source : ECTI-CON <strong>2010</strong> - The <strong>2010</strong> ECTI <strong>International</strong> Conference on Electrical Engineering/Electronics, Computer,<br />
Telecommunications and Information Technology, <strong>2010</strong>, Article number 5491432, Pages 536-539<br />
Document Type : Conference Paper<br />
Keywords : Absorption spectral data, Beverage industry, Chemical vapor, Data analysis, Data groups, Gas exposure, Iso-propanols, Methyl<br />
benzoate, Molecular sensing, Optical electronics, Organic films, Principal components analysis, Product quality, Sensing<br />
films, Sensing layers, Spin-coated thin films, UV-Vis spectrophotometers; Absorption spectroscopy, Acetone, Artificial<br />
organs, Beverages, Chlorine compounds, Data reduction, Ethanol, Gas absorption, Information technology, Infrared<br />
spectrophotometers, Manganese, Manganese compounds, Meteorological instruments, Methanol, Nitrogen<br />
compounds, Porphyrins, Principal component analysis, Spectrophotometers, Spectrophotometry, Spin dynamics, Thin<br />
films, Vapors, Volatile organic compounds, Zinc, Zinc coatings, Zinc compounds; Gas permeable membranes<br />
<strong>Abstract</strong> : The classification <strong>of</strong> product quality based on an optical electronic nose is becoming an instrument <strong>of</strong> much interest in<br />
beverage industry. The optical electronic nose is composed <strong>of</strong> a molecular sensing layer, UV-Vis spectrophotometer and a<br />
data analysis unit. The sensing layer was fabricated from 3 types <strong>of</strong> organic compounds, namely Zinc-2,9,16,23- tetra- tert-<br />
butyl- 29H,31H- phthalocyanine (ZnTTBPc), Zinc-5,10,15,20-tetraphenyl- 21H,23H-porphyrin (ZnTPP) and manganese(III)-<br />
5,10,15,20-tetraphenyl-21H,23H- porphyrin chloride (MnTPPCl) by using the spin-coating technique. The UV-Vis<br />
Spectrophotometer set up was used to detect gases and chemical vapors by measuring changes in the absorption spectrum<br />
<strong>of</strong> the spin-coated thin film upon gas exposure. The sensitivity <strong>of</strong> the organic film was tested by using volatile organic<br />
compounds (VOCs) vapor, for instance, methanol, ethanol, isopropanol, acetone (5% eq.), acetic acid (5% eq.) and methyl<br />
benzoate (5% eq.), which are common VOCs in food and beverages. The Principal Components Analysis (PCA) was then<br />
applied to the absorption spectral data to classify the type <strong>of</strong> the tested chemical vapors. The results indicate that this<br />
sensing film with the three organic compounds produced a good separation <strong>of</strong> data groups according to the chemical type.<br />
No.584<br />
Author (s) : Kladsomboon, S., Puntheeranurak, T., Pratontep, S., Kerdcharoen, T.<br />
Title : An artificial nose based on M-porphyrin (M = Mg, Zn) thin film and optical spectroscopy<br />
Source : INEC <strong>2010</strong> - <strong>2010</strong> 3rd <strong>International</strong> Nanoelectronics Conference, Proceedings, <strong>2010</strong>, Article number 5425096, Pages 968-969<br />
Document Type : Conference Paper<br />
Keywords : Alcoholic beverages, Artificial nose, Classification results, Density functional theory calculations, Food industries, Optical<br />
absorbance, Optical gas sensors, Optical spectroscopy, Polycrystalline structure, Principle component analysis, Sensing<br />
material, UV-vis spectroscopy, X-rays diffraction; Atomic force microscopy, Density functional theory, Flow<br />
patterns, Magnesium, Methanol, Nanoelectronics, Polycrystalline materials, Porphyrins, Quality assurance, Quality control, Thin<br />
films, Total quality management, Ultraviolet spectroscopy, Volatile organic compounds, Zinc, Zinc compounds; Beverages<br />
<strong>Abstract</strong> : Artificial nose has recently become an emerging instrument for quality assurance in the food industry. These paper presents<br />
the optical gas sensors based on Magnesium - 5,10,15,20 - tetra phenyl - porphyrin (MgTPP) and Zinc - 5,10,15,20 - tetra<br />
phenyl- porphyrin (ZnTPP) thin films and their application as an artificial nose. Based on the measurement <strong>of</strong> optical<br />
absorbance response using a general UV-Vis spectroscopy, this artificial noses was tested to discriminate various volatile<br />
organic compounds (VOCs) and Thai beverages. Atomic force microscopy (AFM) and X-rays diffraction were used to confirm<br />
the polycrystalline structure <strong>of</strong> the sensing materials. Density functional theory (DFT) calculations reveal that MgTPP interacts<br />
more strongly with the VOCs than ZnTPP, especially with water and methanol. The classification results <strong>of</strong> VOCs and Thai<br />
beverage vapors using the principle component analysis indicate that both MgTPP and ZnTPP-based artificial noses can be an<br />
efficient tool for quality assurance <strong>of</strong> alcoholic beverages. ©<strong>2010</strong> IEEE.<br />
213
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.585<br />
Author (s) : Klainin, P., Ounnapiruk, L.<br />
Title : A meta-analysis <strong>of</strong> self-care behavior research on elders in thailand: An update<br />
Source : Nursing Science Quarterly, Volume 23, Issue 2, <strong>2010</strong>, Pages 156-163<br />
Document Type : Article<br />
Keywords : Elderly; Orem; Self-care behavior; Thailand<br />
<strong>Abstract</strong> : Self-care is essential for elderly individuals to maintain their optimal level <strong>of</strong> health. This meta-analysis was conducted to<br />
summarize research findings relating to self-care among the elderly in Thailand. Twenty studies undertaken from 1990 to<br />
2008 were used and 328 effect sizes were computed. Most studies were unpublished master's theses guided by Orem's selfcare<br />
deficit theory. Data were collected in these studies by face-to-face interviews. Variables with the largest effect sizes<br />
encompassed self-concept, social support, and self-efficacy. Those with medium effect sizes entailed family relationships,<br />
overall health beliefs, internal locus <strong>of</strong> control, health status, and external locus <strong>of</strong> control. Results from this meta-analysis is<br />
useful information for nursing. © The Author(s) <strong>2010</strong>.<br />
No.586<br />
Author (s) : Klin-eam, C., Suantai, S.<br />
Title : Fixed point theorems for α-nonexpansive mappings<br />
Source : Applied Mathematics Letters, Volume 23, Issue 6, June <strong>2010</strong>, Pages 728-731<br />
Document Type : Article<br />
Keywords : α-nonexpansive mapping; Contraction; Fixed point; Lipschitzian mapping<br />
<strong>Abstract</strong> : In this paper, we prove some fixed point theorems <strong>of</strong> α-nonexpansive mappings introduced by Goebel and Pineda (2007) [1].<br />
Moreover, the demiclosedness principle for theα-nonexpansive mappings is also established. © <strong>2010</strong> Elsevier Ltd. All rights<br />
reserved.<br />
No.587<br />
Author (s) : Kockan, I., Daim, T.U., Gerdsri, N.<br />
Title : Roadmapping future powertrain technologies: A case study <strong>of</strong> Ford Otosan<br />
Source : <strong>International</strong> Journal <strong>of</strong> Technology, Policy and Management, Volume 10, Issue 1-2, June <strong>2010</strong>, Pages 157-184<br />
Document Type : Article<br />
Keywords : Automotive; Decision making; Powertrain; Technology development envelope; Technology roadmap<br />
<strong>Abstract</strong> : This study explores future powertrain systems with the aim <strong>of</strong> defining the most probable implantation road map for the<br />
different alternatives to improve powertrain efficiency. A new methodology called technology development envelope (TDE)<br />
for transforming the roadmapping approach to the level in which it is dynamic, flexible and operationalisable is used for a<br />
case study <strong>of</strong> Ford Otosan's technological planning concept. In the first section, technologic roadmapping methodology is<br />
explained. As a next step, each powertrain solution is explained. Advantages regarding efficiency, fuel consumption, costeffectiveness,<br />
emissions, infrastructure and performance have been listed. In the next sections, TDE methodology and the<br />
application DELPHI method are described in detail. A series <strong>of</strong> criteria and sub-factors have been generated with the aim to<br />
compare the different powertrain systems to identify the best solutions. Copyright © <strong>2010</strong> Inderscience Enterprises Ltd.<br />
No.588<br />
Author (s) : Koh, G.C.K.W., Limmathurotsakul, D.<br />
Title : Gamma interferon supplementation for melioidosis<br />
Source : Antimicrobial Agents and Chemotherapy, Volume 54, Issue 10, October <strong>2010</strong>, Page 4520<br />
Document Type : Letter<br />
Keywords : ceftazidime, gamma interferon, gamma1b interferon; antibiotic therapy, drug effect, letter, low drug<br />
dose, melioidosis, nonhuman, priority journal, survival rate; Animals, Anti-Bacterial Agents, Burkholderia<br />
pseudomallei, Ceftazidime, Cell Division, Disease Models, Animal, Drug Synergism, Drug Therapy,<br />
Combination, Female, Interferon-gamma, Macrophages, Melioidosis, Mice, Mice, Inbred BALB C<br />
<strong>Abstract</strong> : –<br />
No.589<br />
Author (s) : Koh, G.C.K.W., Maude, R.J., Paris, D.H., Newton, P.N., Blacksell, S.D.<br />
214
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Review: Diagnosis <strong>of</strong> scrub typhus<br />
Source : American Journal <strong>of</strong> Tropical Medicine and Hygiene, Volume 82, Issue 3, March <strong>2010</strong>, Pages 368-370<br />
Document Type : Review<br />
Keywords : antiinfective agent, bacterial DNA, beta lactam antibiotic, bacterial antigen; agglutination test, antibody detection, antibody<br />
titer, antigen detection, antigenic variation, antimicrobial therapy, Australia, bacterial strain, bacterial transmission, bacterium<br />
culture, bacterium isolate, base pairing, biosafety, confidence interval, disease surveillance, DNA<br />
extraction, fever, fluorescence microscope, fluorescent antibody technique, gene amplification, gene sequence, gene<br />
targeting, human, immunity, in vitro study, inoculation, Japan, loop isothermal<br />
amplification, Malaysia, mite, mortality, nonhuman, nucleic acid amplification, Orientia tsutsugamushi, polymerase chain<br />
reaction, rash, review, scrub typhus, sensitivity and specificity, serodiagnosis, serology, serotype, South<br />
Korea, standard, Trombiculidae, genetic procedures, immunoassay, microbiology; Acari, Trombiculidae; Antigens,<br />
Bacterial, Fluorescent Antibody Technique, Indirect, Genetic Techniques, Humans, Immunoassay, Scrub Typhus<br />
<strong>Abstract</strong> : Scrub typhus is transmitted by trombiculid mites and is endemic to East and Southeast Asia and Northern Australia. The<br />
clinical syndrome classically consists <strong>of</strong> a fever, rash, and eschar, but scrub typhus also commonly presents as an<br />
undifferentiated fever that requires laboratory confirmation <strong>of</strong> the diagnosis, usually by indirect fluorescent antibody (IFA)<br />
assay. We discuss the limitations <strong>of</strong> IFA, debate the value <strong>of</strong> other methods based on antigen detection and nucleic acid<br />
amplification, and outline recommendations for future study. Copyright © <strong>2010</strong> by The American Society <strong>of</strong> Tropical Medicine<br />
and Hygiene.<br />
No.590<br />
Author (s) : Koh, G.C.K.W.; Maude, R.J.; Paris, D.H.; Newton, P.N.; Blacksell, S.D.<br />
Title : Diagnosis <strong>of</strong> Scrub Typhus<br />
Source : AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, Volume: 82, Issue: 3, Pages: 368-370, DOI: 10.4269/ajtmh.<strong>2010</strong>.09-<br />
0233, Published: MAR <strong>2010</strong><br />
Document Type : Review<br />
Keywords : –<br />
<strong>Abstract</strong> : Scrub typhus is transmitted by trombiculid mites and is endemic to East and Southeast Asia and Northern Australia. The<br />
clinical syndrome classically consists <strong>of</strong> a fever, rash, and eschar, but scrub typhus also commonly presents as an<br />
undifferentiated fever that requires laboratory confirmation <strong>of</strong> the diagnosis, usually by indirect fluorescent antibody (IFA)<br />
assay. We discuss the limitations <strong>of</strong> IFA, debate the value <strong>of</strong> other methods based oil antigen detection and nucleic acid<br />
amplification, and outline recommendations for future study.<br />
No.591<br />
Author (s) : Koletzko, B., Baker, S., Cleghorn, G., Neto, U.F., Gopalan, S., Hernell, O., Hock, Q.S., Jirapinyo, P., Lonnerdal, B., Pencharz, P.,<br />
Pzyrembel, H., Ramirez-Mayans, J., Shamir, R., Turck, D., Yamashiro, Y., Zong-Yi, D.<br />
Title : Global standard for the composition <strong>of</strong> infant formula: Recommendations <strong>of</strong> an ESPGHAN coordinated international expert<br />
group<br />
Source : Japanese Pharmacology and Therapeutics, Volume 38, Issue 8, <strong>2010</strong>, Pages 689-710<br />
Document Type : Review<br />
Keywords : ascorbic acid, calcium, carbohydrate, cyanocobalamin, erucic acid, essential fatty acid, folic<br />
acid, fructose, glucose, iodine, lactose, lauric acid, magnesium, manganese, milk protein, mineral, myristic<br />
acid, nicotinamide, nitrogen, pantothenic acid, phosphorus, retinol, rib<strong>of</strong>lavin, starch, sucrose, thiamine, trace element, trans<br />
fatty acid, vitamin D, vitamin K group; amino acid composition, artificial milk, calorie, food composition, infant<br />
nutrition, international cooperation, lipid composition, medical society, practice guideline, protein<br />
content, review, standard, world health organization<br />
<strong>Abstract</strong> : –<br />
No.592<br />
Author (s) : Kondo, S., Trakoolsomboon, S., Smittipat, N., Juthayothin, T., Palittapongarnpim, P.<br />
Title : Pulsed field gel electrophoresis analysis <strong>of</strong> vibrio cholerae isolates in southern Thailand<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 2, March <strong>2010</strong>, Pages 410-417<br />
Document Type : Article<br />
215
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : PFGE; Southern Thailand; V. cholerae<br />
<strong>Abstract</strong> : Forty isolates <strong>of</strong> V. cholorae O1, O139 and non-O1/non-O139 collected from outbreaks in Songkhla and Phuket Provinces <strong>of</strong><br />
southern Thailand during 1999-2001 and sporadic cases from different regions <strong>of</strong> Thailand during 1993-2002 were<br />
characterized using pulsed field gel electrophoresis (PFGE). Digestion <strong>of</strong> chromosomal DNA <strong>of</strong> the V. cholerae isolates with<br />
restriction endonuclease NotI, followed by PFGE, generated 10 distinct restriction endonuclease analysis patterns consisting <strong>of</strong><br />
8 to 13 bands, ranging in size from 78 to 394 kb. PFGE patterns <strong>of</strong> O1 Inaba strains from the outbreak in Songkhla were<br />
identical (P1) except one isolate (P3). The O1 Inaba outbreak strains from Phuket in the same period belonged to P2 pattern,<br />
whereas the O1 Ogawa strain from the outbreak in Phuket isolated in 1999 was <strong>of</strong> P7 pattern. These patterns <strong>of</strong> O1 Inaba and<br />
Ogawa strains were slightly different suggesting that the isolates were epidemiologically related and therefore the outbreaks<br />
were likely due to the same V. cholerae clone. Isolates <strong>of</strong> V. cholerae O1 Inaba from sporadic cases in the neighboring area<br />
(eg, Pattani Province) in a similar period <strong>of</strong> time <strong>of</strong> the outbreak in Songkhla Province had very similar patterns, with only one<br />
single band different from those <strong>of</strong> the outbreak isolates. This indicates that the Inaba strains isolated from Songkhla Province<br />
during the 2001 cholera outbreak belonging to P1 pattern had not spread to other regions in 2001 and 2002. On the<br />
otherhand, the sporadic isolates collected from other regions <strong>of</strong> Thailand were quite distinct from the outbreak isolates in<br />
Songkhla Province, especially those from Chaiyaphum and Chaing Mai Provinces, which belonged to P5 and P6 pattern,<br />
respectively. Isolates <strong>of</strong> V. cholerae O139 and non-O1/non-O139 gave different patterns from that <strong>of</strong> V. cholerae O1. This<br />
study shows that the PFGE technique is markedly advantageous in distinguishing strains <strong>of</strong> V. cholerae isolates leading to<br />
insightful detailed charateristics <strong>of</strong> these isolates in Thailand.<br />
No.593<br />
Author (s) : Kongchareonsombat, W., Atichoksakun, S., Kitvikai, K., Patcharatrakul, S., Chaimuengraj, S.<br />
Title : Extraperitoneal versus transperitoneal approach <strong>of</strong> laparoscopic ureterolithotomy in selected patients<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 7, July <strong>2010</strong>, Pages 794-798<br />
Document Type : Article<br />
Keywords : Extraperitoneal; Intraperitoneal; Laparoscopic ureterolithotomy; Outcomes<br />
<strong>Abstract</strong> : Objective: To evaluate laparoscopic ureterolithotomy between extraperitoneal and intraperitoneal approach in patients with<br />
failure for Extracoporeal shock wave lithotripsy (ESWL) and endoscopic procedure <strong>of</strong> stone removal. Material and Method: A<br />
retrospective review was performed in 39 patients (40 stone units) underwent laparoscopic ureterolithotomy (extraperitoneal<br />
or intraperitoneal approach) in Ramathibodi Hospital between July 1997 and December 2007. The patients who had a large,<br />
impacted ureteric stone more than 1.5 cm or after failure <strong>of</strong> ESWL and endoscopic procedure were included. The data was<br />
collected and compared in operative time, estimated blood loss (EBL), duration <strong>of</strong> retaining drain (days) and complications.<br />
Results: The EBL, operative time and duration <strong>of</strong> retaining drain were 100 cc, 125.8 minutes and 3 days in the extraperitoneal<br />
group and 51 cc, 128 minutes and 5.8 days in the intraperitoneal group, respectively. The duration <strong>of</strong> retaining drain in the<br />
transperitoneal group was significant longer than the extraperitoneal group (p = 0.002). The EBL, operative time and<br />
complication were not significantly different between the two groups. Conclusion: There does not seem to be a clear<br />
advantage to using a transperitoneal versus extraperitoneal approach for laparoscopic ureterolithotomy, depending on<br />
physician preference.<br />
No.594<br />
Author (s) : Kongkachuichai, R., Charoensiri, R., Sungpuag, P.<br />
Title : Carotenoid, flavonoid pr<strong>of</strong>iles and dietary fiber contents <strong>of</strong> fruits commonly consumed in Thailand<br />
Source : <strong>International</strong> Journal <strong>of</strong> Food Sciences and Nutrition, Volume 61, Issue 5, August <strong>2010</strong>, Pages 536-548<br />
Document Type : Article<br />
Keywords : carotenoids; dietary fiber; flavonoids; Thai fruit<br />
<strong>Abstract</strong> : Soluble and insoluble dietary fiber and flavonoid contents in 21 varieties <strong>of</strong> Thai fruits, as well as carotenoids in five varieties<br />
<strong>of</strong> ripe durians were determined. Fresh fruits were purchased from five local markets in Bangkok during July 2008-May 2009.<br />
Dietary fiber content ranged from 0.71 to 3.58 g/100 g edible portion, with all five varieties <strong>of</strong> durian, guava, ripe banana and<br />
papaya being good sources <strong>of</strong> dietary fiber. Durian (Chanee, Kradom, and Puang manee variety) having yellow to deep-yellow<br />
color pulp had the highest carotenoid content. Durian, pomelo, guava and ripe banana were good sources <strong>of</strong> flavonoids;<br />
especially pomelo (Thong dee and Tuptimsayam variety) showed the greatest total flavonoid content (13,994.21 and<br />
15,094.99 μg/100 g edible portion). Data in this study demonstrated that Thai fruits are not only a good source <strong>of</strong> dietary<br />
fiber but also a good source <strong>of</strong> carotenoids and flavonoids. © <strong>2010</strong> Informa UK Ltd.<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.595<br />
Author (s) : Kongkaew, C., Loykulnant, S., Chaikumpollert, O., Suchiva, K.<br />
Title : Creaming <strong>of</strong> skim natural rubber latex with chitosan derivatives<br />
Source : Journal <strong>of</strong> Applied Polymer Science, Volume 115, Issue 2, 15 January <strong>2010</strong>, Pages 1022-1031<br />
Document Type : Article<br />
Keywords : Biopolymers; Modification; Phase separation; Rubber<br />
<strong>Abstract</strong> : To recover residual rubber from skim natural rubber (SNR) latex, a novel method was developed on the basis <strong>of</strong> the use <strong>of</strong><br />
water-soluble chitosan derivatives. An anionic chitosan derivative, N,O-carboxymethyl chitosan (CMCh), and a cationic chitosan<br />
derivative, N-(2-hydroxy)propyl-3-trimethylammonium chitosan chloride (HTACh), were prepared. It was found that the<br />
creaming process could be achieved with both chitosan derivatives. By the addition <strong>of</strong> 7.34-10.3-g/L CMCh, SNR latex was<br />
almost completely creamed, and no coagulation <strong>of</strong> SNR particles in the cream phase occurred. In the case <strong>of</strong> HTACh, the<br />
creaming process was achieved with a lower concentration (<3.70 g/L) than in the case <strong>of</strong> CMCh, but the size <strong>of</strong> SNR<br />
particles in the cream phase was larger. Solid-state 13C-NMR, thermogravimetric analysis, and f potential measurement results<br />
provided evidence that the creaming <strong>of</strong> SNR latex with CMCh and HTACh was controlled by the depletion flocculation and<br />
adsorption mechanisms, respectively. © 2009 Wiley Periodicals, Inc.<br />
No.596<br />
Author (s) : Kongkathip, B., Sutthiprabha, S., Yoosook, C., Mongkolsook, Y., Kongkathip, N.<br />
Title : Determination <strong>of</strong> a pyranocoumarin and three carbazole compounds in Clausena excavata by RP-HPLC<br />
Source : Journal <strong>of</strong> Chromatographic Science, Volume 48, Issue 6, July <strong>2010</strong>, Pages 445-449<br />
Document Type : Article<br />
Keywords : carbazole derivative, methanol, plant extract, pyranocoumarin derivative; article, chemistry, Clausena, high performance liquid<br />
chromatography, methodology, plant root, reversed phase liquid chromatography, statistical model;<br />
Carbazoles, Chromatography, High Pressure Liquid, Chromatography, Reverse-Phase, Clausena, Linear Models, Methanol, Plant<br />
Extracts, Plant Roots, Pyranocoumarins; Clausena, Clausena excavata, Excavata, Human immunodeficiency virus, Human<br />
immunodeficiency virus 1<br />
<strong>Abstract</strong> : Clausenidin, O-methylmukonal, 3-formyl-2,7-dimethoxycarbazole, and clauszoline-J, isolated from the rhizomes and roots <strong>of</strong><br />
Clausena excavata, exhibit anti-HIV-1 activity in a syncytial assay with EC50 values <strong>of</strong> 5.3, 12.0, 29.1, and 34.2 μM,<br />
respectively. Due to the highly active anti-HIV-1 property, quantitative analysis <strong>of</strong> four compounds are investigated. The direct<br />
analysis <strong>of</strong> these four compounds in the crude extracts <strong>of</strong> the combined rhizomes and roots <strong>of</strong> Clausena excavata from ten<br />
various sources in Thailand by high-performance liquid chromatography is accomplished. Chromatographic separation is<br />
achieved on a C18 column, and the mobile phase is a mixture <strong>of</strong> methanol and distilled water in a mode <strong>of</strong> isocratic or<br />
gradient elution detected at 254 nm at a flow rate <strong>of</strong> 0.6 mL/min for clausenidin, at 274 nm at a flow rate <strong>of</strong> 0.6 mL/min for<br />
O-methylmukonal, at 298 nm at a flow rate <strong>of</strong> 0.4 mL/min for 3-formyl-2,7-dimethoxycarbazole, and at 242 nm at a flow rate<br />
<strong>of</strong> 0.4 mL/min for clauszoline-J. This is the first quantitative analysis <strong>of</strong> these four anti-HIV-1 compounds from the crude<br />
extract without prior isolation and purification steps.<br />
No.597<br />
Author (s) : Kongkathip, N., Pradidphol, N., Hasitapan, K., Grigg, R., Kao, W.-C., Hunte, C., Fisher, N., Warman, A.J., Biagini, G.A., Kongsaeree,<br />
P., Chuawong, P., Kongkathip, B.<br />
Title : Transforming rhinacanthin analogues from potent anticancer agents into potent antimalarial agents<br />
Source : Journal <strong>of</strong> Medicinal Chemistry, Volume 53, Issue 3, <strong>2010</strong>, Pages 1211-1221<br />
Document Type : Article<br />
Keywords : 1,4 naphthoquinone derivative, 3 (1,4 d ihydro 2 hydroxy 1,4 dioxonaphthalen 3 yl) 2,2 dimethylpropyl 2 methylbutanoate, 3<br />
(1,4 dihydro 2 hydroxy 1,4 dioxonaphthalen 3 yl) 2,2 dimethylpropyl 2 ethylbutanoate, 3 (1,4 dihydro 2 hydroxy 1,4<br />
dioxonaphthalen 3 yl) 2,2 dimethylpropyl 2 methyl octanoate, 3 (1,4 dihydro 2 hydroxy 1,4 dioxonaphthalen 3 yl) 2,2<br />
dimethylpropyl 2 methylbutanoate, 3 (1,4 dihydro 2 hydroxy 1,4 dioxonaphthalen 3 yl) 2,2 dimethylpropyl 2<br />
methylpentanoate, 3 (1,4 dihydro 2 hydroxy 1,4 dioxonaphthalen 3 yl) 2,2 dimethylpropyl 3 methylpentanoate, 3 (1,4 dihydro<br />
2 hydroxy 1,4 dioxonaphthalen 3 yl) 2,2 dimethylpropyl acetate, 3 (1,4 dihydro 2 hydroxy 1,4 dioxonaphthalen 3 yl) 2,2<br />
dimethylpropyl butyrate, 3 (1,4 Dihydro 2 hydroxy 1,4 dioxonaphthalen 3 yl) 2,2 dimethylpropyl decanoate, 3 (1,4 dihydro 2<br />
hydroxy 1,4 dioxonaphthalen 3 yl) 2,2 dimethylpropyl dodecanoate, 3 (1,4 dihydro 2 hydroxy 1,4 dioxonaphthalen 3 yl) 2,2<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
dimethylpropyl heptanoate, 3 (1,4 dihydro 2 hydroxy 1,4 dioxonaphthalen 3 yl) 2,2 dimethylpropyl hexanoate, 3 (1,4 dihydro<br />
2 hydroxy 1,4 dioxonaphthalen 3 yl) 2,2 dimethylpropyl isobutyrate, 3 (1,4 dihydro 2 hydroxy 1,4 dioxonaphthalen 3 yl) 2,2<br />
dimethylpropyl isovalerate, 3 (1,4 dihydro 2 hydroxy 1,4 dioxonaphthalen 3 yl) 2,2 dimethylpropyl nonanoate, 3 (1,4 dihydro<br />
2 hydroxy 1,4 dioxonaphthalen 3 yl) 2,2 dimethylpropyl octanoate, 3 (1,4 dihydro 2 hydroxy 1,4 dioxonaphthalen 3 yl) 2,2<br />
dimethylpropyl palmitate, 3 (1,4 dihydro 2 hydroxy 1,4 dioxonaphthalen 3 yl) 2,2 dimethylpropyl pentanoate, 3 (1,4 dihydro 2<br />
hydroxy 1,4 dioxonaphthalen 3 yl) 2,2 dimethylpropyl propionate, 3 (1,4 dihydro 2 hydroxy 1,4 dioxonaphthalen 3 yl) 2,2<br />
dimethylpropyl stearate, 3 (1,4 dihydro 2 hydroxy 1,4 dioxonaphthalen 3 yl) 2,2 dimethylpropyl tetradecanoate, 3 (1,4<br />
dihydro 2 hydroxy 1,4 dioxonaphthalen 3 yl) 2,2 dimethylpropyl undecanoate, 3 (1,4 dihydro 2 hydroxy 1,4 dioxonaphthalen<br />
3 yl)propyl acetate, 3 (1,4 dihydro 2 hydroxy 1,4 dioxonaphthalen 3 yl)propyl butyrate, antimalarial agent, antineoplastic<br />
agent, monomer, rhinacanthin, unclassified drug, unindexed drug; antimalarial activity, article, cytotoxicity, drug synthesis, IC<br />
50, nonhuman, Plasmodium falciparum, rat, Vero cell; Animals, Antimalarials, Antineoplastic Agents, Cell<br />
Survival, Cercopithecus aethiops, Electron Transport Complex III, Erythrocytes, Inhibitory Concentration 50, Malaria,<br />
Falciparum, Male, Mitochondrial Membranes, Naphthoquinones, Plasmodium falciparum, Rats, Rats, Wistar, Saccharomyces<br />
cerevisiae, Structure-Activity Relationship, Vero Cells<br />
<strong>Abstract</strong> : Twenty-six novel naphthoquinone aliphatic esters were synthesized by esterification <strong>of</strong> 1,4-naphthoquinone alcohols with<br />
various aliphatic acids. The 1,4-naphthoquinone alcohols were prepared from 1-hydroxy-2-naphthoic acid in nine steps with<br />
excellent yields. Twenty-four <strong>of</strong> the novel synthetic naphthoquinone esters showed significant antimalarial activity with IC 50<br />
values in the range <strong>of</strong> 0.03-16.63 μM. The length <strong>of</strong> the aliphatic chain and the presence <strong>of</strong> C-2′ substituents on the propyl<br />
chain affected the activity. Interestingly, compounds 31 and 37 showed very good antimalarial activity and were not toxic to<br />
normal Vero cells, and the PTI values <strong>of</strong> 31 (> 1990.38) and 37 (1825.94) are excellent. Both 31 and 37 showed potent<br />
inhibition against P. falciparum 3D7 cyt bc1 and no inhibition on rat cyt bc1. They showed IC50 values in the nanomolar<br />
range, providing full inhibition <strong>of</strong> cyt bc1 with one molecule inhibitor bound per cyt bc1 monomer at the Qo site. © <strong>2010</strong><br />
American Chemical Society.<br />
No.598<br />
Author (s) : Kongkiatpaiboon, S., Gritsanapan, W.<br />
Title : Distribution, bioactive components and biological activities <strong>of</strong> Stemona species in Thailand<br />
Source : Medicinal Plants - <strong>International</strong> Journal <strong>of</strong> Phytomedicines and Related Industries, Volume 2, Issue 1, January <strong>2010</strong>, Pages 1-12<br />
Document Type : Review<br />
Keywords : Antitussive; Insecticide; Stemona; Stemona alkaloids; Stemonaceae; Stilbenoids<br />
<strong>Abstract</strong> : Phytochemical investigations <strong>of</strong> Stemona species have revealed the presence <strong>of</strong> alkaloids, stilbenoids, tocopherols<br />
(chromenols), etc. Stemona alkaloids constitute a unique chemical feature <strong>of</strong> the Stemonaceae and cannot be detected so<br />
far in any other plant families. Their alkaloid structures are characterized by a pyrrolo[1,2-a]azepine core which can be<br />
classified into three types based on biosynthetic consideration i.e. stichoneurine-(tuberostemonine-), protostemonine- and<br />
croomine-types <strong>of</strong> alkaloids. Tuberosa group contains stichoneurine- and croomine-types, demonstrating antitussive activity,<br />
while non-tuberosa group contains protostemonine type alkaloid which promotes potent insecticidal activity. Biological<br />
testings showed that insecticide and antitussive activities <strong>of</strong> Stemona are depended on Stemona alkaloids while antifungal<br />
activity against plant-pathogenic fungi is depended on the presence <strong>of</strong> stilbenoids. Moreover, preliminary tests on anticancer<br />
and antioxidant activities <strong>of</strong> some Stemona species have also been reported.<br />
No.599<br />
Author (s) : Kongkiatpaiboon, S., Keeratinijakal, V., Gritsanapan, W.<br />
Title : Microscopic characterization as a tool for separation <strong>of</strong> Stemona groups<br />
Source : Pharmacognosy Journal, Volume 2, Issue 17, December <strong>2010</strong>, Pages 1-4<br />
Document Type : Article<br />
Keywords : Non Tai Yak; Protostemonine; Stemona; Stemonaceae; Stichoneurine<br />
<strong>Abstract</strong> : Introduction: Stemona plants have been traditionally used as an insecticide, scabicide and pediculocide, and for the<br />
treatment <strong>of</strong> skin and respiratory diseases. Stemona can be separated into two groups according to their morphological<br />
characters and bioactive components i.e. stichoneurine and protostemonine groups. Protostemonine group contains alkaloids<br />
that possess potent insecticidal activity while stichoneurine group accumulates alkaloids with antitussive activity. In Thailand,<br />
a vernacular name "Non Tai Yak" refers to the roots <strong>of</strong> different species <strong>of</strong> Stemona, making it confusing to discern different<br />
species. The purposes <strong>of</strong> this study are to investigate the microscopic characteristics <strong>of</strong> the roots <strong>of</strong> seven species <strong>of</strong> Stemona<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
growing in Thailand and to distinguish and identify these groups <strong>of</strong> Stemona. Methods: Cross-sectional histology <strong>of</strong> fresh root<br />
samples and powdered drug characteristics <strong>of</strong> 7 species <strong>of</strong> Stemona were studied under a microscope. Results: The roots <strong>of</strong><br />
Stemona in the stichoneurine group (S. tuberosa and S. phyllantha) contained a non-lignified large pith while the roots <strong>of</strong><br />
protostemonine group (S. burkillii, S. cochinchinensis, S. collinsiae, S. curtisii and S. kerrii) had a small lignified one. The<br />
powder <strong>of</strong> stichoneurine group contained numerous thin-walled parenchyma, but only few thick-walled parenchyma and<br />
lignified fibers and vessels were present. In contrast, thick-walled parenchyma and lignified fibers and vessels were frequently<br />
found in the powdered roots <strong>of</strong> protostemonine Stemona. These characteristics could be used to discern between Stemona<br />
in the stichoneurine and protostemonine groups. Conclusions: The microscopic characterizations can be used as a primary<br />
tool to categorize and separate 2 main Stemona groups.<br />
No.600<br />
Author (s) : Kongsaeree, P.T., Ratananikom, K., Choengpanya, K., Tongtubtim, N., Sujiwattanarat, P., Porncharoennop, C., Onpium, A., Svasti,<br />
J.<br />
Title : Substrate specificity in hydrolysis and transglucosylation by family 1 β-glucosidases from cassava and Thai rosewood<br />
Source : Journal <strong>of</strong> Molecular Catalysis B: Enzymatic, Volume 67, Issue 3-4, December <strong>2010</strong>, Pages 257-265<br />
Document Type : Article<br />
Keywords : β-Glucosidase; Dalcochinase; Linamarase; Substrate specificity; Transglucosylation<br />
<strong>Abstract</strong> : Thai rosewood (Dalbergia cochinchinensis Pierre) dalcochinase and cassava (Manihot esculenta Crantz) linamarase are<br />
glycoside hydrolase family 1 β-glucosidases with 47% amino acid sequence identity. Each enzyme can hydrolyze its natural<br />
substrate, dalcochinin-8′-O-β-d-glucoside and linamarin, respectively, but not the natural substrate <strong>of</strong> the other enzyme.<br />
Linamarase can transfer glucose to primary, secondary and tertiary alcohols with high efficiency, while dalcochinase can<br />
transglucosylate primary and secondary alcohols at moderate levels. In this study, eight amino acid residues in the aglycone<br />
binding pocket <strong>of</strong> dalcochinase were individually replaced with the corresponding residues <strong>of</strong> linamarase, in order to identify<br />
residues that may account for their catalytic differences. The residues I185 and V255 <strong>of</strong> dalcochinase appeared important for<br />
its substrate specificity, with their respective mutations resulting in 24- and 12-fold reductions in k cat/Km for the hydrolysis <strong>of</strong><br />
dalcochinin-8′-O- β-d-glucoside. Transglucosylation activity was improved when I185, N189 and V255 <strong>of</strong> dalcochinase were<br />
replaced with A201, F205 and F271 <strong>of</strong> linamarase, respectively, suggesting these residues support transglucosylation in<br />
linamarase. Among these three mutants, only the N189F mutant showed significant increases in the rate constants for the<br />
reactivation <strong>of</strong> trapped glucosyl-enzyme intermediates by all alcohols. Together, our results suggest that both hydrophobicity<br />
and geometry are important determinants for substrate specificity in hydrolysis and transglucosylation by these family 1 βglucosidases.<br />
© <strong>2010</strong> Elsevier B.V. All rights reserved.<br />
No.601<br />
Author (s) : Kongtip, P., Borisut, P., Yoosook, W., Osiri, P., Rojanavipart, P.<br />
Title : Development <strong>of</strong> an airborne lead analysis kit and its application<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 6, November <strong>2010</strong>, Pages 1500-1511<br />
Document Type : Article<br />
Keywords : Airborne lead analysis; Colorimetric method<br />
<strong>Abstract</strong> : We developed a method to analyze airborne lead concentrations in the field. It was a modification <strong>of</strong> the colorimetric<br />
method using the reaction between 4(2-pyridylazo)-resorcinol (PAR) and lead with cyanex302 in an acid medium to reduce<br />
interfering metals. The lead concentration was detected with a photometer made in Thailand. The developed method uses<br />
an impinger containing 1% nitric acid solution as an absorbing agent to collect airborne lead at a flow rate <strong>of</strong> less than or<br />
equal to one liter/minute. Cyanex302 solution in toluene was used to extract metals from the samples and 0.1M nitric acid<br />
was used to extract just lead. The lead solution was reacted in 0.5 ml <strong>of</strong> 0.03% PAR solution, with 1 ml ammonium chloride<br />
buffer; the absorption <strong>of</strong> this solution was measured by a photometer. The results show the limit <strong>of</strong> detection (LOD) was 0.01<br />
mg/l. The limit <strong>of</strong> quantification (LOQ) was 0.03 mg/l. The percent recovery <strong>of</strong> the lead concentrations <strong>of</strong> 0.05 - 3.0 mg/l was<br />
94.0 to 103.5%. The precision presented as %CV ranged from 0.65 to 10.27%. Lead concentration in a lead smelting factory<br />
detected by this method was not significantly different from that detected by the NIOSH method: 7,303 at a 95% confidence<br />
level.<br />
No.602<br />
Author (s) : Koomanachai, P., Bulik, C.C., Kuti, J.L., Nicolau, D.P.<br />
219
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Pharmacodynamic modeling <strong>of</strong> intravenous antibiotics against gram-negative bacteria collected in the United States<br />
Source : Clinical Therapeutics, Volume 32, Issue 4, April <strong>2010</strong>, Pages 766-779<br />
Document Type : Article<br />
Keywords : Acinetobacter baumannii; Escherichia coli; Klebsiella pneumoniae; Monte Carlo<br />
simulation; Pharmacodynamic; Pseudomonas aeruginosa<br />
<strong>Abstract</strong> : Background: In the era <strong>of</strong> escalating antimicrobial resistance, the choice <strong>of</strong> effective empiric antimicrobial therapy has become<br />
considerably more difficult. In an attempt to improve antimicrobial selection, pharmacodynamic modeling that considers the<br />
drug, dose, dosing interval, and duration <strong>of</strong> infusion is increasingly used as a tool to assist in the clinical decision-making<br />
process. Objective: The aim <strong>of</strong> the PASSPORT (Probability <strong>of</strong> target attainment <strong>of</strong> Antibacterial agents Studied for<br />
Susceptibility and Pharmacodynamic Optimization in Regional Trials) study was to compare the probabilities <strong>of</strong> achieving<br />
requisite pharmacodynamic exposure (eg, T>MIC, AUC/MIC) <strong>of</strong> common intravenous antibiotics against Escherichia coli,<br />
Klebsiella pneumoniae, Acinetobacter baumannii, and Pseudomonas aeruginosa. Methods: Using a 5000-patient Monte Carlo<br />
simulation, pharmacodynamic analyses were conducted for standard and high-dose, prolonged (ie, 3-to 4-hour) infusions <strong>of</strong><br />
cefepime, ceftazidime, ceftriaxone, cipr<strong>of</strong>loxacin, doripenem, ertapenem, imipenem, lev<strong>of</strong>loxacin, meropenem, and<br />
piperacillin/tazobactam in adult patients with normal renal function (≥50 mL/min). MIC data were incorporated from the 2008<br />
TRUST (Tracking Resistance in the United States Today)-12 surveillance program, a long-running resistance study in 56<br />
participating US hospitals. The cumulative fraction <strong>of</strong> response (CFR) was determined for each regimen against each<br />
population <strong>of</strong> E coli, K pneumoniae, A baumannii, and P aeruginosa. Optimal CFR was defined a priori as ≥90%. Results: All <strong>of</strong><br />
the β-lactam regimens had optimal CFRs against E coli, and all but piperacillin/tazobactam 3.375 g q6h had optimal CFRs<br />
against K pneumoniae. The fluoroquinolones had the lowest CFRs against all <strong>of</strong> the pathogen populations tested (73.2%-<br />
88.9%. against E coli and K pneumortfae; 44.5 %-61.9 % against A baumannii and P aeruginosa). Optimal CFR against A<br />
baumannii was not achieved with any <strong>of</strong> the regimens. Against P aeruginosa, high-dose, prolonged-infusion doripenem and<br />
meropenem had CFRs <strong>of</strong> 97.2% to 98.8%, followed by high-dose, prolonged-infusion ceftazidime (93.3%) and cefepime<br />
(93.2%). High-dose, prolonged-infusion regimens were associated with increased CFRs for all (β-lactams by ~10% over that <strong>of</strong><br />
standard 0.5-hour infusion regimens against the nonfermenting gram-negative bacilli. Conclusions: Based on this model,<br />
standard doses <strong>of</strong> most intravenous (β-lactam regimens had high probabilities <strong>of</strong> achieving optimal exposure against<br />
Enterobacteriaceae. For nonfermenting gram-negative bacilli such as A baumannii and P aeruginosa, high-dose, prolonged<br />
infusions <strong>of</strong> cefepime, ceftazidime, doripenem, and meropenem had the highest probabilities <strong>of</strong> achieving bactericidal<br />
exposure. © <strong>2010</strong> Excerpta Medica Inc.<br />
No.603<br />
Author (s) : Kornsakulkarn, J., Thongpanchang, C., Chainoy, R., Choowong, W., Nithithanasilp, S., Thongpanchang, T.<br />
Title : Bioactive metabolites from cultures <strong>of</strong> basidiomycete favolaschia tonkinensis<br />
Source : Journal <strong>of</strong> Natural Products, Volume 73, Issue 4, 23 April <strong>2010</strong>, Pages 759-762<br />
Document Type : Article<br />
Keywords : 2,3 dihydro 1 benzoxepin derivative, 9 methoxystrobilurin a, 9 methoxystrobilurin b, 9 methoxystrobilurin<br />
g, butenolide, oudemansin a, oudemansin b, strobilurin, unclassified drug; animal cell, antifungal activity, antimalarial<br />
activity, antineoplastic activity, article, Basidiomycetes, controlled study, cytotoxicity, drug isolation, Favolaschia<br />
tonkinensis, fungus culture, human, human cell, IC 50, metabolite, nonhuman; 4-Butyrolactone, Animals, Antifungal<br />
Agents, Antimalarials, Basidiomycota, Benzoxepins, Candida albicans, Cercopithecus aethiops, Cytotoxins, Drug Screening<br />
Assays, Antitumor, Fatty Acids, Unsaturated, Female, Humans, KB Cells, Methacrylates, Microbial Sensitivity Tests, Molecular<br />
Structure, Plasmodium falciparum, Pyrazoles, Pyrimidines, Thailand, Vero Cells; Favolaschia, Fungi<br />
<strong>Abstract</strong> : Two strobilurins, 9-methoxystrobilurin B (1) and 9-methoxystrobilurin G (2), two monochlorinated 2,3-dihydro-1-benzoxepin<br />
derivatives, 3 and 4a, and butenolide 5, together with four known compounds, strobilurin B, 9-methoxystrobilurin A, and<br />
oudemansins A and B, were isolated from culture BCC 18689 <strong>of</strong> the fungus Favolaschia tonkinensis. 9-Methoxystrobilurins A, B<br />
(1), and G (2) and oudemansins A and B exhibited antimalarial, antifungal, and cytotoxic activities, while compounds 3, 4a,<br />
and 5 displayed only cytotoxic activity. © <strong>2010</strong> The American Chemical Society and American Society <strong>of</strong> Pharmacognosy.<br />
No.604<br />
Author (s) : Korsangruang, S., Soonthornchareonnon, N., Chintapakorn, Y., Saralamp, P., Prathanturarug, S.<br />
Title : Effects <strong>of</strong> abiotic and biotic elicitors on growth and is<strong>of</strong>lavonoid accumulation in Pueraria candollei var. candollei and P.<br />
candollei var. mirifica cell suspension cultures<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Source : Plant Cell, Tissue and Organ Culture, Volume 103, Issue 3, December <strong>2010</strong>, Pages 333-342<br />
Document Type : Article<br />
Keywords : Elicitor; Fabaceae; HPLC; Is<strong>of</strong>lavonoid; Medicinal plant; Pueraria candollei; Tissue culture<br />
<strong>Abstract</strong> : This study demonstrates the effects <strong>of</strong> various concentrations <strong>of</strong> abiotic and biotic elicitors on the cell growth and<br />
is<strong>of</strong>lavonoid accumulation <strong>of</strong> P. candollei var. mirifica (PM) and P. candollei var. candollei (PC) cell suspension cultures. The<br />
two plant varieties exhibited different growth responses and varied is<strong>of</strong>lavonoid accumulation after the addition <strong>of</strong> elicitors.<br />
Copper sulfate, methyl jasmonate (MeJA), and yeast extract did not significantly affect the growth <strong>of</strong> either plant variety,<br />
whereas oligosaccharide and the biotic elicitors used in this study [i. e., 50 mg l-1 chitosan and all concentrations <strong>of</strong> laminarin<br />
(LAM)] suppressed the growth <strong>of</strong> PM. The addition <strong>of</strong> MeJA to the medium principally induced an effect on the is<strong>of</strong>lavonoid<br />
content in both PM and PC, with 2.0 μM MeJA inducing the highest is<strong>of</strong>lavonoid content, as indicated by the induction index-<br />
4.41 in PM and 9.62 in PC cells on the 12th and ninth day <strong>of</strong> culture, respectively. A maximum total is<strong>of</strong>lavonoid content <strong>of</strong><br />
40.49 mg g-1 dry weight was achieved in PM 21 days after elicitation with 2.0 μM MeJA. LAM elicited the PM cell suspension<br />
culture to produce puerarin, which was not found in the unelicited culture. The results <strong>of</strong> this study provide information that<br />
will be useful for enhancing the accumulation <strong>of</strong> is<strong>of</strong>lavonoids in P. candollei cell suspension cultures. © <strong>2010</strong> Springer<br />
Science+Business Media B.V.<br />
No.605<br />
Author (s) : Korsangruang, S.; Yamazaki, M.; Saito, K.; Prathanturarug, S.<br />
Title : Cloning <strong>of</strong> gene encoding chalcone isomerase (CHI) from P. candollei and expression <strong>of</strong> genes involved is<strong>of</strong>lavonoid<br />
biosynthesis pathway in seedling plants<br />
Source : PLANTA MEDICA, Volume: 76, Issue: 12, Pages: 1197-1197, Published: AUG <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.606<br />
Author (s) : Kositanont, U., Wongsurakiat, P., Pooruk, P., Maranetra, N., Puthavathana, P.<br />
Title : Induction <strong>of</strong> cross-neutralizing antibody against H5N1 virus after vaccination with seasonal influenza vaccine in COPD patients<br />
Source : Viral Immunology, Volume 23, Issue 3, 1 June <strong>2010</strong>, Pages 329-334<br />
Document Type : Article<br />
Keywords : cross reacting antibody, influenza vaccine, neutralizing antibody, placebo, triple vaccine, virus antibody, vitamin B group;<br />
acute respiratory tract disease, aged, antibody production, antibody response, antibody titer, article, avian influenza, blood<br />
sampling, chronic obstructive lung disease, clinical trial, controlled clinical trial, controlled study, cross reaction, double blind<br />
procedure, drug efficacy, follow up, hemagglutination inhibition, human, immunoassay, influenza A (H1N1), influenza A<br />
(H2N2), influenza A (H3N2), influenza A (H5N1), influenza B, influenza vaccination, Influenza virus A H3N2, Influenza virus A<br />
H5N1, Influenza virus B, major clinical study, respiratory distress, seasonal influenza, seroconversion, serology, virus<br />
neutralization, blood, case control study, hemagglutination inhibition test, immunology, influenza, Influenza virus A<br />
H1N1, longitudinal study, randomized controlled trial, serodiagnosis; Aves, Influenza A virus, Influenza B<br />
virus, Orthomyxoviridae; Aged, Antibodies, Neutralizing, Antibodies, Viral, Case-Control Studies, Cross Reactions, Double-Blind<br />
Method, Hemagglutination Inhibition Tests, Humans, Influenza A Virus, H1N1 Subtype, Influenza A Virus, H3N2<br />
Subtype, Influenza A Virus, H5N1 Subtype, Influenza B virus, Influenza Vaccines, Influenza, Human, Longitudinal<br />
Studies, Neutralization Tests, Placebos, Pulmonary Disease, Chronic Obstructive<br />
<strong>Abstract</strong> : Archival serum samples from elderly individuals with underlying chronic obstructive pulmonary disease (COPD) who were<br />
enrolled in a double-blind case-control study <strong>of</strong> seasonal influenza vaccine efficacy were assayed for cross-neutralizing<br />
antibody formation to avian influenza A (H5N1) virus. Of 118 serum samples, 58 were collected from influenza vaccinees<br />
(mean age 68.5y), and 60 from placebo controls (mean age 68.4y) who received vitamin B injections. Blood samples were<br />
collected before and at 1mo after seasonal influenza vaccination from all subjects; in addition, for a longitudinal follow-up<br />
period <strong>of</strong> 1y paired-blood samples were collected again from subjects who developed acute respiratory illness.<br />
Hemagglutination inhibition assay for antibodies to influenza A (H1N1), influenza A (H3N2), and influenza B viruses was carried<br />
out to determine the serological response to vaccination, and to diagnose influenza viral infection, while microneutralization<br />
assays were performed to detect cross-reactive antibody to H5N1 virus. Pre-existing cross-reactive H5N1 antibody at reciprocal<br />
titer 10 was found in 6 (10.3%) vaccinees and 4 (6.7%) placebo controls. There was no change in H5N1 antibody titer in these<br />
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subjects after vaccination. On the other hand, 3 (5.2%) vaccinees developed seroconversion to H5N1 virus at 1mo after<br />
vaccination, even though they had no pre-existing H5N1 antibody in their first blood samples. No cross-neutralizing antibody<br />
to H5N1 virus was detected in the placebo controls or in the 22 influenza patients, suggesting that influenza vaccination, but<br />
not influenza virus infection, induces cross-neutralizing antibody against avian influenza H5N1 virus. © <strong>2010</strong>, Mary Ann Liebert,<br />
Inc.<br />
No.607<br />
Author (s) : Kozono, S., Matsuyama, T., Biwasa, K.K., Kawahara, K.-i., Nakajima, Y., Yoshimoto, T., Yonamine, Y., Kadomatsu, H., Tancharoen,<br />
S., Hashiguchi, T., Noguchi, K., Maruyama, I.<br />
Title : Involvement <strong>of</strong> the endocannabinoid system in periodontal healing<br />
Source : Biochemical and Biophysical Research Communications, Volume 394, Issue 4, 16 April <strong>2010</strong>, Pages 928-933<br />
Document Type : Article<br />
Keywords : Cannabinoid receptors; Endocannabinoids; Gingival fibroblasts; Mitogen-activated protein kinases; Proliferation; Wound<br />
healing<br />
<strong>Abstract</strong> : Endocannabinoids including anandamide (AEA) and 2-arachidonoylglycerol (2-AG) are important lipid mediators for<br />
immunosuppressive effects and for appropriate homeostasis via their G-protein-coupled cannabinoid (CB) receptors in<br />
mammalian organs and tissues, and may be involved in wound healing in some organs. The physiological roles <strong>of</strong><br />
endocannabinoids in periodontal healing remain unknown. We observed upregulation <strong>of</strong> the expression <strong>of</strong> CB1/CB2 receptors<br />
localized on fibroblasts and macrophage-like cells in granulation tissue during wound healing in a wound-healing model in<br />
rats, as well as an increase in AEA levels in gingival crevicular fluid after periodontal surgery in human patients with<br />
periodontitis. In-vitro, the proliferation <strong>of</strong> human gingival fibroblasts (HGFs) by AEA was significantly attenuated by AM251 and<br />
AM630, which are selective antagonists <strong>of</strong> CB1 and CB2, respectively. CP55940 (CB1/CB2 agonist) induced phosphorylation <strong>of</strong><br />
the extracellular-regulated kinases (ERK) 1/2, p38 mitogen-activated protein kinase (p38MAPK), and Akt in HGFs. Wound<br />
closure by CP55940 in an in-vitro scratch assay was significantly suppressed by inhibitors <strong>of</strong> MAP kinase kinase (MEK),<br />
p38MAPK, and phosphoinositol 3-kinase (PI3-K). These findings suggest that endocannabinoid system may have an important<br />
role in periodontal healing. © <strong>2010</strong> Elsevier Inc. All rights reserved.<br />
No.608<br />
Author (s) : Kraipeerapun, P., Amornsamankul, S.<br />
Title : Ensemble <strong>of</strong> duo output neural networks for binary classification<br />
Source : <strong>International</strong> Journal <strong>of</strong> Mathematics and Computers in Simulation, Volume 4, Issue 4, <strong>2010</strong>, Pages 162-170<br />
Document Type : Article<br />
Keywords : Binary classification problem; Complementary neural networks; Ensemble neural network; Feed forward back propagation<br />
neural network; Uncertainty<br />
<strong>Abstract</strong> : This paper presents an ensemble <strong>of</strong> duo output neural networks (DONN) using bagging technique to solve binary classification<br />
problems. DONN is a neural network that is trained to predict a pair <strong>of</strong> complementary outputs which are the truth and falsity<br />
values. Each component in an ensemble contains two DONNs in which the first network is trained to predict the truth and<br />
falsity outputs whereas the second network is trained to predict the falsity and truth outputs which are set in reverse order <strong>of</strong><br />
the first one. In this paper, we propose classification techniques based on outputs obtained from DONNs. Also, the ensemble<br />
selection technique is proposed. This technique is created based on uncertainty and diversity values. All proposed<br />
techniques have been tested with three benchmarks UCI data sets, which are ionosphere, pima, and liver. It is found that the<br />
proposed ensemble techniques provide better results than those obtained from an ensemble <strong>of</strong> back propagation neural<br />
networks, an ensemble <strong>of</strong> complementary neural networks, a single pair <strong>of</strong> duo output neural networks, a single pair <strong>of</strong><br />
complementary neural networks, and a back propagation neural network.<br />
No.609<br />
Author (s) : Kraipeerapun, P., Amornsamankul, S.<br />
Title : Using Duo Output Neural Network to solve binary classification problems<br />
Source : <strong>International</strong> Conference on Applied Computer Science - Proceedings, <strong>2010</strong>, Pages 286-290<br />
Document Type : Conference Paper<br />
Keywords : Binary classification; Complementary neural network; Duo Output Neural Network; Feedforward backpropagation neural<br />
network<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : This paper proposes an approach to solve binary classification problems using Duo Output Neural Network (DONN). DONN is a<br />
neural network trained to predict a pair <strong>of</strong> complementary outputs which are the truth and falsity values. In this paper,<br />
outputs obtained from two DONNs are aggregated and used to predict the classification result. The first DONN is trained to<br />
predict a pair <strong>of</strong> truth and falsity values. The second DONN is trained to predict a pair <strong>of</strong> falsity and truth values. The target<br />
outputs used to train the second network are organized in reverse order <strong>of</strong> the first network. The proposed approach has<br />
been tested with three benchmarking UCI data sets, which are ionosphere, pima, and liver. It is found that the proposed<br />
techniques improve the performance as compared to feedforward backprogation neural network and complementary neural<br />
network.<br />
No.610<br />
Author (s) : Kraipeerapun, P., Nakkrasae, S., Fung, C.C., Amornsamankul, S.<br />
Title : Solving regression problem with complementary neural networks and an adjusted averaging technique<br />
Source : Memetic Computing, Volume 2, Issue 4, December <strong>2010</strong>, Pages 249-257<br />
Document Type : Article<br />
Keywords : Complementary neural networks; Feed-forward backpropagation neural network; Regression problem; Uncertainty<br />
<strong>Abstract</strong> : This research deals with complementary neural networks (CMTNN) for the regression problem. Complementary neural<br />
networks consist <strong>of</strong> a pair <strong>of</strong> neural networks called truth neural network and falsity neural network, which are trained to<br />
predict truth and falsity outputs, respectively. In this paper, a novel adjusted averaging technique is proposed in order to<br />
enhance the result obtained from the basic CMTNN. We test our proposed technique based on the classical benchmark<br />
problems including housing, concrete compressive strength, and computer hardware data sets from the UCI machine learning<br />
repository. We also realize our technique to the porosity prediction problem based on well log data set obtained from<br />
practical field data in the oil and gas industry. We found that our proposed technique provides better performance when<br />
compared to the traditional CMTNN, backpropagation neural network, and support vector regression with linear, polynomial,<br />
and radial basis function kernels. © <strong>2010</strong> Springer-Verlag.<br />
No.611<br />
Author (s) : Krajaejun, T., Chongtrakool, P., Angkananukul, K., Brandhorst, T.T.<br />
Title : Effect <strong>of</strong> temperature on growth <strong>of</strong> the pathogenic oomycete pythium insidiosum<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 6, November <strong>2010</strong>, Pages 1462-1466<br />
Document Type : Article<br />
Keywords : Growth; Oomycete; Pythiosis; Pythium insidiosum; Temperature<br />
<strong>Abstract</strong> : Pythium insidiosum causes a potentially life-threatening infectious disease called pythiosis. An early, accurate diagnosis is<br />
important, since prompt treatment leads to a better prognosis. Unsuccessful attempts to isolate the organism have been<br />
associated with specimens subjected to lower temperatures. We analyzed growth <strong>of</strong> P. insidiosum at various temperatures.<br />
Culture at low (8°C) and high (42°C) temperatures resulted in death or inhibited growth <strong>of</strong> the organism. Culture under<br />
optimal temperatures (28 and 32°C) was important for successful isolation <strong>of</strong> P. insidiosum.<br />
No.612<br />
Author (s) : Krajaejun, T., Keeratijarut, A., Sriwanichrak, K., Lowhnoo, T., Rujirawat, T., Petchthong, T., Yingyong, W., Kalambaheti, T.,<br />
Smittipat, N., Juthayothin, T., Sullivan, T.D.<br />
Title : The 74-kilodalton immunodominant antigen <strong>of</strong> the pathogenic oomycete Pythium insidiosum is a putative exo-1,3-βglucanase<br />
Source : Clinical and Vaccine Immunology, Volume 17, Issue 8, August <strong>2010</strong>, Pages 1203-1210<br />
Document Type : Article<br />
Keywords : fungus antigen, glucan 1,3 beta glucosidase; article, enzyme analysis, enzyme linked immunosorbent assay, mass<br />
spectrometry, nonhuman, nucleotide sequence, phylogeny, Phytophthora infestans, polymerase chain reaction, priority<br />
journal, pythiosis, Pythium, pythium insidiosum; Algal Proteins, Animals, Cluster Analysis, DNA Primers, DNA,<br />
Algal, Genotype, Glucan 1,3-beta-Glucosidase, Humans, Immunodominant Epitopes, Mass Spectrometry, Molecular<br />
Weight, Phytophthora infestans, Polymerase Chain Reaction, Pythium, Sequence Analysis, DNA, Sequence Homology, Amino<br />
Acid, Sequence Homology, Nucleic Acid<br />
<strong>Abstract</strong> : The oomycetous, fungus-like, aquatic organism Pythium insidiosum is the causative agent <strong>of</strong> pythiosis, a life-threatening<br />
infectious disease <strong>of</strong> humans and animals living in tropical and subtropical areas <strong>of</strong> the world. Common sites <strong>of</strong> infection are<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
the arteries, eyes, cutaneous/subcutaneous tissues, and gastrointestinal tract. Diagnosis <strong>of</strong> pythiosis is time-consuming and<br />
difficult. Radical excision <strong>of</strong> the infected organs is the main treatment for pythiosis because conventional antifungal drugs are<br />
ineffective. An immunotherapeutic vaccine prepared from P. insidiosum crude extract showed limited efficacy in the<br />
treatment <strong>of</strong> pythiosis patients. Many pythiosis patients suffer lifelong disabilities or die from an advanced infection. Recently,<br />
we identified a 74-kDa major immunodominant antigen <strong>of</strong> P. insidiosum which could be a target for development <strong>of</strong> a more<br />
effective serodiagnostic test and vaccines. Mass spectrometric analysis identified two peptides <strong>of</strong> the 74-kDa antigen (s74-1<br />
and s74-2) which perfectly matched a putative exo-1,3-β-glucanase (EXO1) <strong>of</strong> Phytophthora infestans. Using degenerate<br />
primers derived from these peptides, a 1.1-kb product was produced by PCR, and its sequence was found to be homologous<br />
to that <strong>of</strong> the P. infestans exo-1,3-β-glucanase gene, EXO1. Enzyme-linked immunosorbent assays targeting the s74-1 and<br />
s74-2 synthetic peptides demonstrated that the 74-kDa antigen was highly immunoreactive with pythiosis sera but not with<br />
control sera. Phylogenetic analysis using part <strong>of</strong> the 74-kDa protein-coding sequence divided 22 Thai isolates <strong>of</strong> P. insidiosum<br />
into two clades. Further characterization <strong>of</strong> the putative P. insidiosum glucanase could lead to new diagnostic tests and to<br />
antimicrobial agents and vaccines for the prevention and management <strong>of</strong> the serious and life-threatening disease <strong>of</strong> pythiosis.<br />
Copyright © <strong>2010</strong>, American Society for Microbiology. All Rights Reserved.<br />
No.613<br />
Author (s) : Krajaejun, T., Wüthrich, M., Gauthier, G.M., Warner, T.F., Sullivan, T.D., Klein, B.S.<br />
Title : Discordant influence <strong>of</strong> Blastomyces dermatitidis yeast-phase-specific gene BYS1 on morphogenesis and virulence<br />
Source : Infection and Immunity, Volume 78, Issue 6, June <strong>2010</strong>, Pages 2522-2528<br />
Document Type : Article<br />
Keywords : Blastomyces yeast phase specific 1, fungal protein, green fluorescent protein, unclassified drug; agar medium, amino acid<br />
sequence, amino terminal sequence, animal cell, animal experiment, animal model, article, Blastomyces<br />
dermatitidis, blastomycosis, cell culture, cell wall, controlled study, fungal morphology, fungal spore germination, fungal<br />
strain, fungal virulence, fungus hyphae, gene expression, gene silencing, in vitro study, in vivo<br />
study, inoculation, lung, morphogenesis, mouse, nonhuman, pathogenicity, priority journal, signal<br />
transduction, temperature, yeast, yeast cell; Animals, Blastomyces, Colony Count, Microbial, Fungal Proteins, Gene<br />
Silencing, Genes, Fungal, Hyphae, Lung, Mice, Mice, Inbred C57BL, Virulence<br />
<strong>Abstract</strong> : Blastomyces dermatitidis is a thermally induced dimorphic fungus capable <strong>of</strong> causing lung and systemic infections in<br />
immunocompetent animal hosts. With the publication <strong>of</strong> genomic sequences from three different strains <strong>of</strong> B. dermatitidis<br />
and the development <strong>of</strong> RNA interference as a gene-silencing tool, it has become possible to easily ascertain the virulence<br />
and morphological effects <strong>of</strong> knocking down the expression <strong>of</strong> candidate genes <strong>of</strong> interest. BYS1 (Blastomyces yeast-phasespecific<br />
1), first identified by Burg and Smith, is expressed at high levels in yeast cells and is undetectable in mold. The<br />
deduced protein sequence <strong>of</strong> BYS1 has a putative signal sequence at its N terminus, opening the possibility that the BYS1encoded<br />
protein is associated with the yeast cell wall. Herein, strains <strong>of</strong> B. dermatitidis with silenced expression <strong>of</strong> BYS1 were<br />
engineered and tested for morphology and virulence. The silenced strains produced rough-surfaced cultures on agar medium<br />
and demonstrated a propensity to form pseudohyphal cells on prolonged culture in vitro and in vivo, as measured in the<br />
mouse lung. Tests using a mouse model <strong>of</strong> blastomycosis with either yeast or spore inocula showed that the bys1-silenced<br />
strains were as virulent as control strains. Thus, although silencing <strong>of</strong> BYS1 alters morphology at 37°C, it does not appear to<br />
impair the pathogenicity <strong>of</strong> B. dermatitidis. Copyright © <strong>2010</strong>, American Society for Microbiology. All Rights Reserved.<br />
No.614<br />
Author (s) : Kriengsoontornkij, W., Homcheon, B., Chomchai, C., Neamsomboon, W.<br />
Title : Accuracy <strong>of</strong> pediatric triage at Siriraj Hospital, Bangkok, Thailand<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 10, October <strong>2010</strong>, Pages 1172-1176<br />
Document Type : Article<br />
Keywords : Nurse; Pediatric triage; Performance; Work-year<br />
<strong>Abstract</strong> : Background: Siriraj Hospital is a busy 2,500-bed hospital located in Bangkok, Thailand. It has over 1.7 million outpatients,<br />
including 120,000 emergency room visits a year, 20,000 <strong>of</strong> which are pediatric patients. The Pediatric Triage (Pedtriage) system<br />
has been in used since the year 2001, but the factors that affect the performance <strong>of</strong> triage nurse have not been evaluated.<br />
Objective: To compare the performance non-pediatric nurses who are responsible for pediatric patients in the emergency<br />
room before and after pediatric triage training at Siriraj Hospital. Material and Method: Pediatric Triage Training was set up for<br />
emergency room and outpatient department nurses between June and October 2006. The training consisted <strong>of</strong> 5 hours <strong>of</strong><br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
didactic sessions on the concepts <strong>of</strong> pediatric triage and 4-5 hour sessions where the nurses were allowed to triage actual<br />
pediatric patients under the supervision <strong>of</strong> a triage-training nurse. A pre-test and post-test examination was administered. The<br />
outcome <strong>of</strong> triage performance was categorized into under-triage if the patient had an urgent or emergent condition and was<br />
triaged as non-urgent, over-triage if a patient had a non-urgent condition and was triaged as urgent or emergent. Statistical<br />
description included percent, averages, and standard deviation where appropriate. A standard 4x4 contingency table was<br />
used to calculate the sensitivity and specificity. For comparison <strong>of</strong> performance, a post-hoc analysis was done where the<br />
nurses were divided into two groups, those with work experience <strong>of</strong> less than or equal to 5 years (group 1) and more than 5<br />
years (group 2). An independent samples t-test was used to determine the difference in performance between the two<br />
groups. Results: Overall, performance on pre-test - post-test differed significantly before and after training. The nurses in<br />
Group 1 had higher pre-test scores (Group 1 mean = 62.35%, Group 2 mean = 52.41%, p-value = 0.001), were less likely to<br />
overtriage (Group 1 mean = 4.11%, Group 2 mean = 6.46%, p-value = 0.021) and had higher specificity <strong>of</strong> triage than Group 2<br />
(Group 1 mean = 95.61, Group 2 = 92.39, p-value = 0.019). However, the nurses in Group 2 had more improvement in their<br />
post-test scores (percent <strong>of</strong> improvement from pre-test: Group 1 mean = 8.56%, Group 2 = 34.69%, p-value = 0.005).<br />
Conclusion: Work experience is an important consideration in the triage knowledge and performance <strong>of</strong> non-pediatric nurses<br />
during triage training.<br />
No.615<br />
Author (s) : Krings, T., Geibprasert, S., terBrugge, K.<br />
Title : Classification and Endovascular Management <strong>of</strong> Pediatric Cerebral Vascular Malformations<br />
Source : Neurosurgery Clinics <strong>of</strong> North America, Volume 21, Issue 3, July <strong>2010</strong>, Pages 463-482<br />
Document Type : Review<br />
Keywords : Classification; Endovascular management; Pediatric cerebral vascular malformations<br />
<strong>Abstract</strong> : Pediatric vascular malformations <strong>of</strong> the central nervous system differ from those seen in adults. Their classification may be<br />
based on symptoms, pathomechanics, patient's age, morphologic features, or presumed etiology. This review describes the<br />
different classification schemes and the endovascular management options <strong>of</strong> these rare and challenging diseases. The<br />
proposed etiologic classification <strong>of</strong> pediatric vascular malformations may add to our understanding <strong>of</strong> these diseases in<br />
general because the phenotypic expression <strong>of</strong> a given vascular malformation can shed light on the nature and timing <strong>of</strong> the<br />
causative agent, thereby potentially opening up treatment modalities in the future that are directed against the triggering<br />
event rather than against the clinical manifestations or the morphologic appearance. With current endovascular methods,<br />
most vascular diseases can be approached safely and with good clinical results. © <strong>2010</strong> Elsevier Inc.<br />
No.616<br />
Author (s) : Krings, T., Geibprasert, S., TerBrugge, K.G.<br />
Title : Pathomechanisms and treatment <strong>of</strong> pediatric aneurysms<br />
Source : Child's Nervous System, Volume 26, Issue 10, October <strong>2010</strong>, Pages 1309-1318<br />
Document Type : Review<br />
Keywords : Aneurysm; Dissection; Infection; Subarachnoid hemorrhage; Trauma<br />
<strong>Abstract</strong> : Pediatric aneurysms are rare diseases distinct from classical adult aneurysms and therefore require different treatment<br />
strategies. Apart from saccular aneurysms that are more commonly found in older children, three major pathomechanisms<br />
may be encountered: trauma, infection, and dissection. The posterior circulation and more distal locations are more<br />
commonly encountered in children compared to adults, and there is an overall male predominance. Clinical findings are not<br />
only confined to subarachnoid hemorrhage but may also comprise mass effects, headaches or neurological deficits. In<br />
traumatic aneurysms, the skull base and distal anterior communicating artery are commonly affected, and the hemorrhage<br />
occurs <strong>of</strong>ten delayed (2-4 weeks following the initial trauma). Infectious aneurysms are mostly bacterial in origin, and<br />
hemorrhage occurs early after a septic embolic shower. Dissecting aneurysms are the most <strong>of</strong>ten encountered aneurysm type<br />
in children and can lead to mass effect, hemorrhage, or ischemia depending on the fate <strong>of</strong> the intramural hematoma.<br />
Treatment strategies in pediatric aneurysms include endosaccular coil treatment only for the "classical berry-type" aneurysms;<br />
in the other instances, parent vessel occlusion, flow reversal, surgical options, or a combined treatment with bypass and<br />
parent vessel occlusion have to be contemplated. © 2009 Springer-Verlag.<br />
No.617<br />
Author (s) : Krings, T., Hans, F.-J., Geibprasert, S., Terbrugge, K.<br />
225
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Partial "targeted" embolisation <strong>of</strong> brain arteriovenous malformations<br />
Source : European Radiology, Volume 20, Issue 11, November <strong>2010</strong>, Pages 2723-2731<br />
Document Type : Review<br />
Keywords : Brain AVM; Embolisation; Epilepsy; Haemorrhage; Pathomechanism<br />
<strong>Abstract</strong> : The treatment <strong>of</strong> pial arteriovenous brain malformations is controversial. Little is yet known about their natural history, their<br />
pathomechanisms and the efficacy and risks <strong>of</strong> respective proposed treatments. It is known that only complete occlusion <strong>of</strong><br />
the AVM can exclude future risk <strong>of</strong> haemorrhage and that the rates <strong>of</strong> curative embolisation <strong>of</strong> AVMs with an acceptable<br />
periprocedural risk are around 20 to 50%. As outlined in the present article, however, partial, targeted embolisation also plays<br />
a role. In acutely ruptured AVMs where the source <strong>of</strong> bleeding can be identified, targeted embolisation <strong>of</strong> this compartment<br />
may be able to secure the AVM prior to definitive treatment. In unruptured symptomatic AVMs targeted treatment may be<br />
employed if a defined pathomechanism can be identified that is related to the clinical symptoms and that can be cured with<br />
an acceptable risk via an endovascular approach depending on the individual AVM angioarchitecture. This review article gives<br />
examples <strong>of</strong> pathomechanisms and angioarchitectures that are amenable to this kind <strong>of</strong> treatment strategy. © <strong>2010</strong> The<br />
Author(s).<br />
No.618<br />
Author (s) : Krisnangkura, K., Sansa-Ard, C., Aryusuk, K., Lilitchan, S., Kittiratanapiboon, K.<br />
Title : An empirical approach for predicting kinematic viscosities <strong>of</strong> biodiesel blends<br />
Source : Fuel, Volume 89, Issue 10, <strong>2010</strong>, Pages 2775-2780<br />
Document Type : Article<br />
Keywords : Biodiesel; Enthalpy; Fatty acid methyl ester; Free energy; Viscosity<br />
<strong>Abstract</strong> : Kinematic viscosity (η) is an important property <strong>of</strong> diesel fuels, including biodiesels, which are marketed mostly as the blends<br />
in many countries around the world. In this study, the free energy <strong>of</strong> viscous flow (∆G vis) for a non-associated liquid mixture<br />
is assumed to be the summed <strong>of</strong> ∆Gvis <strong>of</strong> individual components. Hence, the Eyring's equation, η = Ae(-∆Gvis/RT), is<br />
transformed to ln ηblend = a + bn1 + c/T + dn1/T (where, a, b, c and d, T and n1 are thermodynamically related constants,<br />
absolute temperature and mole fraction <strong>of</strong> biodiesel, respectively). The transformed equation is used to predict kinematic<br />
viscosity <strong>of</strong> biodiesel blends (ηblend) <strong>of</strong> different degree <strong>of</strong> blending at any temperatures from pour point to 100 °C. The<br />
predicted kinematic viscosities are in good agreement with those reported in literatures at all temperatures. The highest<br />
deviation is ±5.4% and the average absolute deviation (AAD) is less than 2.86%. The transformed equation can also be used<br />
to predict kinematic viscosities <strong>of</strong> pure fatty acid methyl esters in diesel fuel. Methyl ricinoleate is an exception. The AAD is<br />
4.50% and the deviation is as high as 12.80%. The high deviation suggests that molecular interactions between methyl<br />
ricinoleate and diesel fuel is high and cannot be ignored. © 2009 Elsevier Ltd. All rights reserved.<br />
No.619<br />
Author (s) : Krittayaphong, R., Boonyasirinant, T., Chaithiraphan, V., Maneesai, A., Saiviroonporn, P., Nakyen, S., Thanapiboonpol, P.,<br />
Yindeengam, A., Udompanturak, S.<br />
Title : Prognostic value <strong>of</strong> late gadolinium enhancement in hypertensive patients with known or suspected coronary artery disease<br />
Source : <strong>International</strong> Journal <strong>of</strong> Cardiovascular Imaging, Volume 26, Issue SUPPL. 1, February <strong>2010</strong>, Pages 123-131<br />
Document Type : Article<br />
Keywords : Cardiac magnetic resonance; Hypertension; Late gadolinium enhancement; Myocardial infarction<br />
<strong>Abstract</strong> : To determine the prognosis <strong>of</strong> a myocardial scar assessed by a late gadolinium enhancement (LGE) technique <strong>of</strong> cardiac<br />
magnetic resonance (CMR) in hypertensive patients with known or suspected coronary artery disease (CAD). Patients with<br />
systemic hypertension with known or suspected CAD without a clinical history <strong>of</strong> myocardial infarction were enrolled. All<br />
patients underwent CMR for assessment <strong>of</strong> cardiac function and LGE. Prognostic data was determined by the occurrence <strong>of</strong> a<br />
hard cardiac endpoint, defined as cardiac death or a non-fatal myocardial infarction, or major adverse cardiac events (MACEs),<br />
defined as cardiac death, a non-fatal myocardial infarction, or hospitalization due to heart failure, unstable angina, or lifethreatening<br />
ventricular arrhythmia. A total <strong>of</strong> 1,644 patients were enrolled; 48% were males and the mean age was 65 ± 11<br />
years. The average follow-up time was 863 ± 559 days. Four hundred fifty-three (28%) patients had LGE. LGE was the<br />
strongest and most independent predictor for hard events and MACEs with hazard ratios <strong>of</strong> 4.77 and 3.38, respectively. Other<br />
independent predictors <strong>of</strong> hard events and MACEs were left ventricular ejection fraction and mass, the use <strong>of</strong> a beta-blocker,<br />
and a history <strong>of</strong> heart failure. The risk <strong>of</strong> cardiac events increased as the extent <strong>of</strong> LGE increased; the hazard ratio was 12.74<br />
226
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
for hard events for those with a LGE >20% <strong>of</strong> the myocardium. LGE is the most important and independent predictor for<br />
cardiac events in hypertensive patients with known or suspected CAD. © <strong>2010</strong> Springer Science+Business Media, B.V.<br />
No.620<br />
Author (s) : Krityakiarana, W., Espinosa-Jeffrey, A., Ghiani, C.A., Zhao, P.M., Topaldjikian, N., Gomez-Pinilla, F., Yamaguchi, M., Kotchabhakdi,<br />
N., De Vellis, J.<br />
Title : Voluntary exercise increases oligodendrogenesis in spinal cord<br />
Source : <strong>International</strong> Journal <strong>of</strong> Neuroscience, Volume 120, Issue 4, April <strong>2010</strong>, Pages 280-290<br />
Document Type : Article<br />
Keywords : Exercise; Nestin; Neurogenesis; Oligodendrocyte<br />
<strong>Abstract</strong> : Exercise has been shown to increase hippocampal neurogenesis, but the effects <strong>of</strong> exercise on oligodendrocyte generation<br />
have not yet been reported. In this study, we evaluated the hypothesis that voluntary exercise may affect neurogenesis, and<br />
more in particular, oligodendrogenesis in the thoracic segment <strong>of</strong> the intact spinal cord <strong>of</strong> adult nestinGFP transgenic mice.<br />
Voluntary exercise for 7 and 14 days increased nestinGFP expression around the ependymal area. In addition, voluntary<br />
exercise for 7 days significantly increased nestinGFP expression in both the white and gray matter <strong>of</strong> the thoracic segment <strong>of</strong><br />
the intact spinal cord, whereas, 14-day exercise decreased nestinGFP expression. Markers for immature oligodendrocytes<br />
(transferrin and CNPase) were significantly increased after 7 days <strong>of</strong> voluntary exercise. These results suggest that voluntary<br />
exercise positively influences oligodendrogenesis in the intact spinal cord, emphasizing the beneficial effects <strong>of</strong> voluntary<br />
exercise as a possible co-treatment for spinal cord injury.<br />
No.621<br />
Author (s) : Krityakiarana, W.; Espinosa-Jeffrey, A.; Zhao, P. M.; Gomez-Pinilla, F.; Kotchabhakdi, N.; Yamaguchi, M.; de Vellis, J.<br />
Title : TS1 AS EARLY NEUROPROTECTIVE TREATMENT AFTER SPINAL CORD INJURY IN MICE<br />
Source : JOURNAL OF NEUROCHEMISTRY, Volume: 115, Supplement: 1, Pages: 53-53, Published: OCT <strong>2010</strong><br />
Document Type : Meeting Absstract<br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.622<br />
Author (s) : Krockenberger, M.B., Malik, R., Ngamskulrungroj, P., Trilles, L., Escandon, P., Dowd, S., Allen, C., Himmelreich, U., Canfield, P.J.,<br />
Sorrell, T.C., Meyer, W.<br />
Title : Pathogenesis <strong>of</strong> pulmonary Cryptococcus gattii infection: A rat model<br />
Source : Mycopathologia, Volume 170, Issue 5, November <strong>2010</strong>, Pages 315-330<br />
Document Type : Article<br />
Keywords : Cryptococcosis; Cryptococcus; Gattii; Lung; Meningitis; Rat<br />
<strong>Abstract</strong> : A model <strong>of</strong> pulmonary cryptococcosis in immunocompetent rats was developed to better understand the virulence <strong>of</strong><br />
Cryptococcus gattii. Six isolates were studied, representing four molecular genotypes (VGI-MATα, VGIIa-MATα, VGIIa-MATa,<br />
VGIIb-MATα), obtained from Australia, Vancouver (Canada) and Colombia. These originated from human patients, a cat and<br />
the environment and were administered intratracheally (i.t.) or transthoracically into Fischer 344 or Wistar-Furth rats in doses<br />
varying from 104 to 107 colony-forming units (CFU) in 0.1 ml <strong>of</strong> saline. With the exception <strong>of</strong> animals given the VGIIa-MATa<br />
isolate, rats consistently became ill or died <strong>of</strong> progressive cryptococcal pneumonia following i.t. doses exceeding 107 CFU.<br />
Affected lungs increased in weight up to tenfold and contained numerous circumscribed, gelatinous lesions. These became<br />
larger and more extensive, progressing from limited hilar and/or tracheal lesions, to virtually confluent gelatinous masses.<br />
Disease was localized to the lungs for at least 3-4 weeks, with dissemination to the brain occurring in some animals after day<br />
29. The dose-response relationship was steep for two VGI isolates studied (human WM179, environmental WM276); doses up<br />
to 106 CFU i.t. did not produce lesions, while 107 or more yeast cells produced progressive pneumonia. Intratracheal<br />
inoculation <strong>of</strong> rats with C. gattii provides an excellent model <strong>of</strong> human pulmonary cryptococcosis in healthy hosts, mimicking<br />
natural infections. Disease produced by C. gattii in rats is distinct from that caused by C. ne<strong>of</strong>ormans in that infections are<br />
progressive and ultimately fatal. © <strong>2010</strong> Springer Science+Business Media B.V.<br />
No.623<br />
Author (s) : Krongtaew, C., Messner, K., Ters, T., Fackler, K.<br />
227
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Characterization <strong>of</strong> key parameters for biotechnological lignocellulose conversion assessed by FT-NIR spectroscopy. Part I:<br />
Qualitative analysis <strong>of</strong> pretreated straw<br />
Source : BioResources, Volume 5, Issue 4, November <strong>2010</strong>, Pages 2063-2080<br />
Document Type : Article<br />
Keywords : Fourier-transform near infrared spectroscopy (FT-NIR); Lignocellulose characterization; Mild acidic and alkaline peroxide<br />
pretreatments; Multivariate data analysis; Principal component analysis (PCA); Qualitative discriminant analysis; Wheat and<br />
oat straw<br />
<strong>Abstract</strong> : Wheat straw (Triticum aestivum L.) and oat straw (Avena sativa L.) were subjected to acid and alkaline pre-treatments partly<br />
in combination with hydrogen peroxide. The aim was to remove lignin and increase the accessibility <strong>of</strong> the polysaccharides to<br />
enzymatic digestion. Accessibility was evaluated by digestion with a cell wall degrading enzyme complex to yield reducing<br />
sugars that may serve as precursor substrates for bi<strong>of</strong>uels or building block chemicals. Changes in lignin, hemicelluloses, as<br />
well as amorphous, semi-crystalline, and crystalline regions <strong>of</strong> cellulose moieties <strong>of</strong> pretreated straw were efficiently<br />
characterized by Fourier transform near-infrared (FT-NIR) reflectance spectroscopy. These alterations <strong>of</strong> the chemical structure<br />
<strong>of</strong> straw after different pre-treatment methods were powerfully differentiated by principal component analysis (PCA).<br />
Characteristics <strong>of</strong> the different samples owing to the different pretreatment methods could be clustered from the PCA<br />
loadings spectra.<br />
No.624<br />
Author (s) : Krongtaew, C., Messner, K., Ters, T., Fackler, K.<br />
Title : Characterization <strong>of</strong> key parameters for biotechnological lignocellulose conversion assessed by FT-NIR spectroscopy. Part II:<br />
Quantitative analysis by partial least squares regression<br />
Source : BioResources, Volume 5, Issue 4, November <strong>2010</strong>, Pages 2081-2096<br />
Document Type : Article<br />
Keywords : Anaerobic fermentation; Biogas potential test; Fourier transform near-infrared spectroscopy (FT-NIR); Multivariate data<br />
analysis; Partial least-squares (PLS) regression; Wheat and oat straw pre-treatment<br />
<strong>Abstract</strong> : Wheat straw (Triticum aestivum L.) and oat straw (Avena sativa L.) were chemically pretreated at different severities with the<br />
purpose <strong>of</strong> delignification, which in turn leads to a better accessibility <strong>of</strong> plant cell wall polysaccharides for further<br />
biotechnological conversion. Key parameters <strong>of</strong> these samples, i.e. weight loss, residual lignin content, and hydrolysable<br />
sugars serving as precursors for bi<strong>of</strong>uel production were monitored by wet-chemistry analyses. Fourier transform near infrared<br />
(FT-NIR) spectra were correlated to these data by means <strong>of</strong> partial leastsquares (PLS) regression. Weight loss (4.0 - 33.5%) <strong>of</strong><br />
the wheat straw could be predicted (RMSEP = 3.5%, R2test = 0.75) from the entire FT-NIR spectra (10000 - 4000 cm-1).<br />
Residual lignin content (7.9 - 20.7%, RMSEP = 0.9%, R2test = 0.94) and amount <strong>of</strong> reducing sugars based on pretreated wheat<br />
straw (128 - 1000 mg g-1, RMSEP = 83 mg g-1, R2test = 0.89) were powerfully evaluated between 6900 and 5510 cm-1, a<br />
spectral region where polysaccharides and lignin absorb. All these parameters could be equally predicted with even higher<br />
accuracy from pre-treated oat straw samples. Furthermore, some important parameters for anaerobic conversion <strong>of</strong> wheat<br />
straw to biogas - biogas production, total solids, and volatile solids content - could be estimated.<br />
No.625<br />
Author (s) : Kruavit, A., Visuthikosol, V.<br />
Title : Temporoparietal fascial free flap for correction <strong>of</strong> first web space atrophy<br />
Source : Microsurgery, Volume 30, Issue 1, January <strong>2010</strong>, Pages 8-12<br />
Document Type : Article<br />
Keywords : adult, article, atrophy, brachial plexus anesthesia, clinical article, donor site, female, first web space atrophy, follow up, free<br />
tissue graft, general anesthesia, human, leprosy, local anesthesia, male, nerve injury, patient satisfaction, postoperative<br />
period, priority journal, surgical technique, temporoparietal fascial free flap, ulnar nerve paralysis; Adult, Cohort<br />
Studies, Female, Forehead, Hand Deformities, Acquired, Humans, Male, Microsurgery, Middle Aged, Muscular<br />
Atrophy, Retrospective Studies, Scalp, Surgical Flaps, Treatment Outcome, Ulnar Neuropathies<br />
<strong>Abstract</strong> : Fourteen temporoparietal fascial free flaps were used for correction <strong>of</strong> first web space atrophy from ulnar nerve palsy in 13<br />
patients. Ten sustained ulnar nerve injuries and three suffered from leprosy. The procedures were performed under general<br />
anesthesia except one leprosy patient with bilateral ulnar nerve palsy in which local anesthesia and brachial block were<br />
employed to harvest bilateral free flaps and recipient site preparations, respectively. The follow-up time varied from 4 to 64<br />
months. The postoperative results were satisfactory and there was no resorption <strong>of</strong> the free flaps. The consistency <strong>of</strong> the<br />
228
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
augmented first web space was s<strong>of</strong>t and compressible like natural feel. The size <strong>of</strong> the flap was more than enough for<br />
augmentation <strong>of</strong> first web space and donor site morbidity was minimal and accepted by all patients. We conclude that<br />
temporoparietal fascial free flap is an ideal autogenous tissue for correction <strong>of</strong> first web space atrophy. © 2009 Wiley-Liss, Inc.<br />
No.626<br />
Author (s) : Kruaysawat, W., Aekplakorn, W., Chapman, R.S.<br />
Title : Survival time and prognostic factors <strong>of</strong> oral cancer in Ubon Ratchathani Cancer Center<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 3, March <strong>2010</strong>, Pages 278-284<br />
Document Type : Article<br />
Keywords : Oral cancer; Prognostic factors; Survival time<br />
<strong>Abstract</strong> : Objective: To characterize the survival time and prognostic factors <strong>of</strong> oral cancer in Ubon Ratchathani, Thailand. Material and<br />
Method: A total <strong>of</strong> 519 patients with oral cancer in the Ubon Ratchathani Cancer Center were recruited retrospectively over 5<br />
years, from January 1, 2002 to December 31, 2006. The survival status <strong>of</strong> the patients was followed until December 31, 2007.<br />
Survival times were estimated and compared using the product-limit (Kaplan-Meier) method. Cox Proportional Hazards<br />
models were used to examine prognostic factors. Results: At the end <strong>of</strong> the study, 384 patients (74.0%) had died. The mean<br />
age <strong>of</strong> the patients at diagnosis was 64.15 years, with a male to female ratio <strong>of</strong> 1:1.56. Location <strong>of</strong> cancer were found at<br />
tongue (25.2%), buccal mucosa (22.4%), gum (21.1%), lip (17.5%) and others (13.7%). Squamous cell carcinoma was the most<br />
common cell type (92.7%). The median survival time was 337 days and the survival probability at 1, 3 and 5-years were<br />
46.7%, 26.4% and 18.2%, respectively. In multivariable analysis, patients at the greatest risk <strong>of</strong> death were those having<br />
cancer <strong>of</strong> the tongue (HR 1.93, 1.20, 3.11) compared to cancer <strong>of</strong> the lip and being in stage IV at diagnosis (HR 3.57, 95% CI =<br />
1.79, 7.13) as compared to stage I. Conclusion: Patients with advanced tumors had the worst prognosis, underscoring the<br />
importance <strong>of</strong> improved early detection for early treatment.<br />
No.627<br />
Author (s) : Krudsood, S., Tangpukdee, N., Wilairatana, P., Pothipak, N., Duangdee, C., Warrell, D.A., Looareesuwan, S.<br />
Title : Intravenous ibupr<strong>of</strong>en (IV-ibupr<strong>of</strong>en) controls fever effectively in adults with acute uncomplicated Plasmodium falciparum<br />
malaria but prolongs parasitemia<br />
Source : American Journal <strong>of</strong> Tropical Medicine and Hygiene, Volume 83, Issue 1, July <strong>2010</strong>, Pages 51-55<br />
Document Type : Article<br />
Keywords : artesunate, ibupr<strong>of</strong>en, mefloquine; adult, area under the curve, article, body temperature, clinical trial, controlled clinical<br />
trial, controlled study, double blind procedure, drug efficacy, drug safety, drug tolerability, female, fever, human, major<br />
clinical study, malaria falciparum, male, Plasmodium falciparum, randomized controlled trial; Acute<br />
Disease, Adult, Animals, Antimalarials, Artemisinins, Double-Blind Method, Drug Therapy,<br />
Combination, Fever, Humans, Ibupr<strong>of</strong>en, Injections, Intravenous, Malaria, Falciparum, Mefloquine, Parasitemia, Plasmodium<br />
falciparum, Treatment Outcome; Artesunate, Plasmodium falciparum<br />
<strong>Abstract</strong> : Because some febrile patients are unable to swallow or retain oral antipyretic drugs, we carried out a double-blind, placebocontrolled<br />
trial in which intravenous ibupr<strong>of</strong>en (IV-ibupr<strong>of</strong>en) was given to adults hospitalized with fever associated with acute<br />
uncomplicated falciparum malaria treated with oral artesunate plus mefloquine. Thirty patients received IV-ibupr<strong>of</strong>en 400 mg<br />
and 30 received placebo every 6 hours for 72 hours. Reduction in the area above 37.0°C versus time curve was significantly<br />
greater for IV-ibupr<strong>of</strong>en than for placebo during the first 72 hours after first administration. No patients developed severe<br />
malaria; parasite clearance was delayed in the patients whose fevers were controlled by IV-ibupr<strong>of</strong>en (median 37.3 hours<br />
versus 23.7 hours in the placebo group [P = 0.0024]). This difference did not appear to be clinically important Adverse events,<br />
none considered severe, occurred equally in both groups. IV-ibupr<strong>of</strong>en was effective and well tolerated in reducing fever in<br />
febrile inpatients with malaria. Copyright © <strong>2010</strong> by The American Society <strong>of</strong> Tropical Medicine and Hygiene.<br />
No.628<br />
Author (s) : Kruevaisayawan, H., Vanichviriyakit, R., Weerachatyanukul, W., Withyachumnarnkul, B., Chavadej, J., Sobhon, P.<br />
Title : Oogenesis and formation <strong>of</strong> cortical rods in the black tiger shrimp, Penaeus monodon<br />
Source : Aquaculture, Volume 301, Issue 1-4, 23 March <strong>2010</strong>, Pages 91-98<br />
Document Type : Article<br />
Keywords : Cortical rods; Egg; Oogenesis; Ovary; Penaeus monodon<br />
229
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : This study aimed to investigate morphological changes <strong>of</strong> developing oocytes during oogenesis in the black tiger shrimp<br />
Penaeus monodon as well as elucidate the cellular pathway for the formation <strong>of</strong> cortical rods, the egg jelly precursor. The<br />
differentiating female germ cells in the mature ovary were divided into four stages (Oc1-4) based on the changes in<br />
ultrastructural characteristics. The prominent features in the developing oocytes (Oc1 Oc2 Oc3) were the abundance <strong>of</strong><br />
ribosomes and dilated rough endoplasmic reticulum (RER) in Oc1 and Oc2 and the presence <strong>of</strong> yolk granules and lipid<br />
droplets in Oc3 all indicating active synthesis <strong>of</strong> protein and lipid components. The main characteristic <strong>of</strong> the mature oocyte<br />
(Oc4) was the presence <strong>of</strong> cortical rods (CRs) which were composed <strong>of</strong> the tightly packed structural units each resembling a<br />
bottle-brush. Immunostaining <strong>of</strong> cortical rod proteins which were part <strong>of</strong> the structural units indicated that they were first<br />
synthesized in the RER-Golgi complex <strong>of</strong> Oc2 and transported into the extracellular crypts <strong>of</strong> the mature oocyte (Oc4) where<br />
they were assembled into CRs. © <strong>2010</strong> Elsevier B.V. All rights reserved.<br />
No.629<br />
Author (s) : Krumkamp, R., Mounier-Jack, S., Ahmad, A., Reintjes, R., Coker, R.<br />
Title : Evaluating health systems' preparedness for emerging infectious diseases: A novel conceptual and analytic framework<br />
Source : Health Policy, Volume 98, Issue 2-3, December <strong>2010</strong>, Pages 91-97<br />
Document Type : Review<br />
Keywords : Health system; Intervention programmes; Pandemic planning; Policy evaluation<br />
<strong>Abstract</strong> : In this article we present a novel conceptual framework for systematically assessing the national health system capacity to<br />
respond to pandemic influenza. This framework helps to determine how health systems and pandemic programmes interact,<br />
whether, where and which weak points exist, and how and where pandemic response health programmes can be improved<br />
effectively. This new conceptual framework draws upon two existing approaches for assessment and evaluation, the Systemic<br />
Rapid Assessment Toolkit (SYSRA) and the Hazard Analysis <strong>of</strong> Critical Control Points (HACCP). SYSRA is a systematic approach<br />
to analyse the interplay between communicable disease programmes and the broader health systems context within which<br />
they operate and the HACCP methodology builds upon a systematic analysis <strong>of</strong> process steps within a programme in order to<br />
identify likely hazards and to develop control measures to address them. The analytical framework that we have developed<br />
from the novel conceptualisation is simple, can be applied rapidly, and should, we believe, be low cost to implement. Thus,<br />
this provides a means for developing a contextual understanding <strong>of</strong> the broader health system in which a pandemic<br />
infectious disease programme operates, and for identifying frailties in programmes that need to be responded to. © <strong>2010</strong><br />
Elsevier Ireland Ltd.<br />
No.630<br />
Author (s) : Kuadkitkan, A., Wikan, N., Fongsaran, C., Smith, D.R.<br />
Title : Identification and characterization <strong>of</strong> prohibitin as a receptor protein mediating DENV-2 entry into insect cells<br />
Source : Virology, Volume 406, Issue 1, October <strong>2010</strong>, Pages 149-161<br />
Document Type : Article<br />
Keywords : Aedes; Culex; Dengue; Prohibitin; Receptor<br />
<strong>Abstract</strong> : Dengue is transmitted primarily by mosquitoes <strong>of</strong> the Aedes genus. Despite a number <strong>of</strong> studies, no insect dengue virus<br />
receptor protein has been clearly identified and characterized. Using a number <strong>of</strong> separation methodologies and virus overlay<br />
protein binding assays we identified a 35. kDa protein that segregated with susceptibility to dengue serotype 2 (DENV-2)<br />
infection in two mosquito species and two mosquito cell lines. Mass spectroscopy identified the protein to be prohibitin, a<br />
strongly conserved and ubiquitously expressed protein in eukaryotic cells. Antibody mediated inhibition <strong>of</strong> infection and<br />
siRNA mediated knockdown <strong>of</strong> prohibitin expression significantly reduced infection levels and subsequent virus production in<br />
both Aedes aegypti and Aedes albopictus cell lines. Confocal microscopy showed a significant degree <strong>of</strong> intracellular<br />
colocalization between prohibitin and DENV-2 E protein, and coimmunoprecipitation confirmed that prohibitin interacts with<br />
dengue E. Prohibitin is the first characterized insect cell expressed dengue virus receptor protein. © <strong>2010</strong> Elsevier Inc.<br />
No.631<br />
Author (s) : Kuanpradit, C., Cummins, S.F., Degnan, B.M., Sretarugsa, P., Hanna, P.J., Sobhon, P., Chavadej, J.<br />
Title : Identification <strong>of</strong> an attractin-like pheromone in the mucus-secreting hypobranchial gland <strong>of</strong> the abalone haliotis asinina<br />
Linnaeus<br />
Source : Journal <strong>of</strong> Shellfish Research, Volume 29, Issue 3, November <strong>2010</strong>, Pages 699-704<br />
Document Type : Conference Paper<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : abalone; attractin; Haliotis asinina; hypobranchial gland; mucus; pheromone<br />
<strong>Abstract</strong> : Pheromones are chemicals used to communicate between animals <strong>of</strong> the same species, and are thought to be used by most<br />
marine animals. With limited vision, abalone primarily sense their world chemically, and pheromones may play an important<br />
role in settlement, attraction, recognition, alarm, and reproduction. Despite this, there has been no detailed investigation into<br />
pheromone substances, both in their precise biochemical nature or pheromonal function. In this study, we investigated the<br />
presence <strong>of</strong> pheromonelike substances from the hypobranchial gland <strong>of</strong> the abalone Haliotis asinina using bioassays,<br />
immunohistochemistry, Western blotting, and reverse-phase high-performance liquid chromatography (RP-HPLC). The<br />
hypobranchial gland <strong>of</strong> many prosobranchial marine molluscs has been classified as a sex auxiliary gland releasing unknown<br />
substances during spawning. In our study, cephalic tentacle assays demonstrated that the cell extracts <strong>of</strong> the hypobranchial<br />
gland contain chemical cues that are sensed by conspecifics. An antibody against the sea slug "attractin" pheromone was<br />
used as a probe to localize a similar protein in the mucin-secreting cells <strong>of</strong> the epithelial lining the hypobranchial gland <strong>of</strong><br />
both male and female abalone. The approximate molecular weight <strong>of</strong> this abalone attractin-like protein is 30 kDa in both<br />
males and females. Fractionation <strong>of</strong> hypobranchial gland extracts by C5 RP-HPLC could not selectively purify this protein, and<br />
no sex-specific differences were observed. We predict that the attractin-like protein could be one <strong>of</strong> a number <strong>of</strong> important<br />
proteins involved in maturation, aggregation, and/or spawning behavior <strong>of</strong> abalone. In future research, additional<br />
hypobranchial gland components will be tested further for these types <strong>of</strong> behavior.<br />
No.632<br />
Author (s) : Kulkeaw, K., Ishitani, T., Kanemaru, T., Fucharoen, S., Sugiyama, D.<br />
Title : Cold exposure down-regulates zebrafish hematopoiesis<br />
Source : Biochemical and Biophysical Research Communications, Volume 394, Issue 4, 16 April <strong>2010</strong>, Pages 859-864<br />
Document Type : Article<br />
Keywords : Hematopoiesis; Temperature; Zebrafish<br />
<strong>Abstract</strong> : Erythropoiesis is regulated such that a sufficient number <strong>of</strong> mature erythrocytes is produced. Down-regulation <strong>of</strong><br />
erythropoiesis causes various types <strong>of</strong> anemia. Although some anemia-related genes have been identified, there are several<br />
types <strong>of</strong> anemic disease for which the molecular mechanisms are yet unclear, suggesting that unidentified genes in addition<br />
to the classical cytokine pathways play important roles in anemia. To address this issue, a new animal model for anemia is<br />
required. We established a reversible anemic model in zebrafish by keeping fish at 17 °C, a low water temperature. In<br />
zebrafish kidney marrow, expression <strong>of</strong> several genes encoding hematopoietic transcription factors (Runx1, scl, c-myb and<br />
GATA-2) and particularly erythropoiesis-related factors (klfd, hbaa1, ba1, GATA-1, EPO, and EPOr) was down-regulated, whereas<br />
myelopoiesis-related factors (csf1a and csf3) was up-regulated in low temperature conditions. We propose that this zebrafish<br />
model is useful to identify novel genes for hematopoiesis, particularly erythropoiesis. © <strong>2010</strong> Elsevier Inc. All rights reserved.<br />
No.633<br />
Author (s) : Kulthanan, K., Wongkamchai, S., Triwongwaranat, D.<br />
Title : Mosquito allergy: Clinical features and natural course<br />
Source : Journal <strong>of</strong> Dermatology, Volume 37, Issue 12, December <strong>2010</strong>, Pages 1025-1031<br />
Document Type : Article<br />
Keywords : clinical features; mosquito allergy; natural course<br />
<strong>Abstract</strong> : Mosquito bite allergic reactions are due to sensitization to mosquito salivary proteins. The purpose <strong>of</strong> our study was to assess<br />
the clinical features <strong>of</strong> Thai patients with mosquito allergy, and to define the natural desensitization course. Seventy patients<br />
with mosquito allergy were enrolled. Most patients were female. Half <strong>of</strong> the patients had a personal history <strong>of</strong> atopy. The<br />
average age <strong>of</strong> onset <strong>of</strong> mosquito reaction was 5.7 years old (range 2-58). Four patients were in the desensitization phase in<br />
which clinical symptoms disappeared within 9.5 years (median). The common cutaneous lesions were erythematous papules<br />
(68.6%) and immediate wheals (67.1%). The most common area <strong>of</strong> involvement was the leg. Forty <strong>of</strong> 50 patients (80%) had<br />
positive skin prick testing to mosquito allergen (Culex quinquefasciatus) and 31 <strong>of</strong> 42 patients (73.8%) had positive<br />
immunoglobulin E antibodies against allergens in C. quinquefasciatus saliva proteins. © <strong>2010</strong> Japanese Dermatological<br />
Association.<br />
No.634<br />
Author (s) : Kumsiri, R., Potup, P., Chotivanich, K., Petmitr, S., Kalambaheti, T., Maneerat, Y.<br />
231
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Blood stage plasmodium falciparum antigens induce T cell independent immunoglobulin production via B cell activation<br />
factor <strong>of</strong> the TNF family (BAFF) pathway<br />
Source : Acta Tropica, Volume 116, Issue 3, December <strong>2010</strong>, Pages 217-226<br />
Document Type : Article<br />
Keywords : B cells; BAFF; Hemozoin; Immunoglobulin; Plasmodium falciparum<br />
<strong>Abstract</strong> : T independent (TI) antigens (Ags) activate monocytes to produce a cytokine, termed B cell activation factor (BAFF), involved in<br />
immunoglobulin (Ig) production. This study aimed to investigate whether the soluble schizont fraction <strong>of</strong> Plasmodium<br />
falciparum antigen (sPfAg) and hemozoin (HZ) could act as TI Ag to induce P. falciparum (Pf) specific Ig production via BAFF<br />
pathway. Co-cultures <strong>of</strong> monocytes and naïve B cells from 6 healthy donors were stimulated with sPfAg (10 mg/ml) or HZ (10<br />
μM). At interval times, the expressions <strong>of</strong> BAFF on activated monocytes, BAFF receptor (BAFF-R) and proliferation nuclear Ag<br />
in activated B cells were determined by flow cytometry. The soluble BAFF (sBAFF), total and specific IgG levels in the<br />
supernatants were assessed by enzyme-linked immunosorbent assay (ELISA). The finding revealed both sPfAg and HZ could<br />
activate monocytes to express BAFF on surface and release sBAFF in the supernatant within 72 h <strong>of</strong> stimulation. The B cells<br />
responded to specific activation, indicated by BAFF-R expression on the surface within 72 h, marked proliferation on day 7,<br />
and final production <strong>of</strong> total and specific IgG during days 7-12. Comparing to sPfAg, HZ stimulated monocyte and B cell coculture<br />
to express higher levels <strong>of</strong> BAFF and sBAFF during 24-48 h, more BAFF-R on HZ activated B cells within 24 h and<br />
induced marked proliferation <strong>of</strong> B cells with higher Pf specific IgG level. However, stimulation with sPfAg showed a more<br />
significant correlation between BAFF expression on the activated monocytes at 72 h and the Pf specific IgG level on day 12 (r<br />
= 0.961, p = 0.039, Pearson Correlation). In conclusion, it is possible that both sPfAg and HZ stimulated B cells to produce<br />
specific IgG with BAFF involvement. © <strong>2010</strong> Elsevier B.V.<br />
No.635<br />
Author (s) : Kunacheva, C., Fujii, S., Tanaka, S., Boontanon, S.K., Poothong, S., Wongwatthana, T., Shivakoti, B.R.<br />
Title : Perfluorinated compounds contamination in tap water and bottled water in Bangkok, Thailand<br />
Source : Journal <strong>of</strong> Water Supply: Research and Technology - AQUA, Volume 59, Issue 5, <strong>2010</strong>, Pages 345-354<br />
Document Type : Article<br />
Keywords : Bottled water; Perfluorinated compounds; PFOA; PFOS; Tap water; Water treatment plant<br />
<strong>Abstract</strong> : Perfluorinated compounds (PFCs) such as perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) have been<br />
detected in the environment, in biota and in humans. The exposure pathways <strong>of</strong> these chemicals to humans are unclear. Tap<br />
water and bottled water are two possible pathways <strong>of</strong> PFCs occurrence in human blood. The major objectives <strong>of</strong> the study<br />
were to identify the occurrences <strong>of</strong> PFCs in tap and bottled water and to evaluate conventional water treatment processes<br />
performance on removal <strong>of</strong> PFCs. Solid phase extraction coupled with HPLC-ESI-MS/MS were used for the analysis <strong>of</strong> ten<br />
PFCs. PFCs were detected in all tap water samples and bottled water samples. The average PFOS and PFOA concentrations in<br />
tap water were 0.17 and 3.58 ng l-1, respectively. PFOS and PFOA were not similarly distributed in all areas in the city. PFCs<br />
concentrations were higher in bottled water than in tap water. Moreover, the current treatment processes were not effective<br />
in removing PFCs in aqueous phase. Nevertheless, PFCs in particulate phase were effectively removed by primary<br />
sedimentation and rapid sand filtration. Based on the guideline from the New Jersey Department <strong>of</strong> Environmental Protection,<br />
PFOA concentrations in tap water and bottled water found in Bangkok were not expected to cause any health risks. © <strong>2010</strong><br />
IWA Publishing.<br />
No.636<br />
Author (s) : Kungpanichkul, N., Vannasaeng, S., Thongtang, N.<br />
Title : Comparison <strong>of</strong> efficacy between 1 mg overnight dexamethasone suppression test and 2-day low dose dexamethasone<br />
suppression test for diagnosis <strong>of</strong> Cushing's syndrome<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 1, January <strong>2010</strong>, Pages 27-33<br />
Document Type : Article<br />
Keywords : Cushing's syndrome; Dexamethasone suppression test; Diagnostic test<br />
<strong>Abstract</strong> : Objective: To assess the sensitivity and specificity <strong>of</strong> 1 mg overnight dexamethasone suppression test (ODST) and the 2-day<br />
low dose dexamethasone suppression test (LDST) for diagnosis <strong>of</strong> Cushing's syndrome, and to compare the accuracy <strong>of</strong> both<br />
tests using cut<strong>of</strong>f value at below 5 μg/dl and at below 1.8 μg/dl. Material and Method: The present study is a retrospective<br />
study, from 1971-2007, in one academic center <strong>of</strong> 77 patients with clinical suspicion <strong>of</strong> Cushing's syndrome. Kappa statistical<br />
analysis was used to determine agreement between the two tests. Sensitivity and specificity <strong>of</strong> the tests were calculated.<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
ROC curves were created to determine the best cut<strong>of</strong>f value <strong>of</strong> the two tests. Results: ODST has very good agreement with<br />
the more troublesome LDST and has comparable efficacy. Lowering the cut<strong>of</strong>f value from 5 μg/dl to 1.8 μg/dl does not<br />
improve the accuracy <strong>of</strong> ODST but results in decreased specificity <strong>of</strong> LDST. The best cut<strong>of</strong>f value <strong>of</strong> ODST test is ≥ 5.3 μg/dl<br />
and the best cut<strong>of</strong>f value <strong>of</strong> LDST is ≥ 5 μg/dl. Conclusion: ODST is an efficient method for diagnosis <strong>of</strong> patients suspected <strong>of</strong><br />
having Cushing's syndrome. The attempt to lowering cut<strong>of</strong>f value does not improve the efficacy <strong>of</strong> dexamethasone<br />
suppression test.<br />
No.637<br />
Author (s) : Kunkeaw, S., Tangphatsornruang, S., Smith, D.R., Triwitayakorn, K.<br />
Title : Genetic linkage map <strong>of</strong> cassava (Manihot esculenta Crantz) based on AFLP and SSR markers<br />
Source : Plant Breeding, Volume 129, Issue 1, February <strong>2010</strong>, Pages 112-115<br />
Document Type : Article<br />
Keywords : AFLP; Cassava; Linkage map; SSR<br />
<strong>Abstract</strong> : To generate a genetic linkage map <strong>of</strong> cassava (Manihot esculenta Crantz), 58 F1 progenies from a cross between Rayong 90<br />
(female) and Rayong 5 (male) were examined in amplification fragment length polymorphism (AFLP) and simple sequence<br />
repeat (SSR) analyses. A total <strong>of</strong> 469 polymorphic markers consisting <strong>of</strong> 378 AFLPs generated from 76 primer combinations<br />
and 91 SSRs were identified. These markers were analyzed using the JOINMAP® 3.0 package program to construct a genetic<br />
linkage map. A total <strong>of</strong> 33 linkage groups <strong>of</strong> a common map were constructed from 119 AFLPs and 18 SSRs, spanning 1095<br />
cM with an average <strong>of</strong> 7.99 cM between markers. The genetic linkage map generated in this study will be useful for genetic<br />
studies in cassava particularly for the identification <strong>of</strong> genetic markers linked to traits <strong>of</strong> interest, although the complex<br />
cassava genome suggests that maybe a long term objective. © 2009 Blackwell Verlag GmbH.<br />
No.638<br />
Author (s) : Kunkeaw, S., Worapong, J., Smith, D.R., Triwitayakorn, K.<br />
Title : An in vitro detached leaf assay for pre-screening resistance to anthracnose disease in cassava (Manihot esculenta Crantz)<br />
Source : Australasian Plant Pathology, Volume 39, Issue 6, <strong>2010</strong>, Pages 547-550<br />
Document Type : Article<br />
Keywords : Colletotrichum, Glomerella cingulata, Manihot esculenta, Solanum tuberosum<br />
<strong>Abstract</strong> : Cassava anthracnose disease (CAD), caused by Colletotrichum gloeosporioides f. sp. manihotis is one <strong>of</strong> the most important<br />
fungal infections that affects cassava yield in many countries especially in Africa and including Thailand. In this study, a rapid<br />
screening method to identify cassava varieties that are resistant to CAD based on a detached leaf assay was developed. Three<br />
different varieties <strong>of</strong> cassava commonly grown in Thailand, Hanatee, Huay Bong 60 and Kasetsart 50 were used. Agar plugs<br />
(0.8 cm diameter) with mycelia <strong>of</strong> C. gloeosporioides f. sp. manihotis were applied to the centre <strong>of</strong> the middle lobe <strong>of</strong> the<br />
cassava leaves, while sterile potato dextrose agar plugs were used as controls. Lesions on the inoculated samples were<br />
measured on the fourth day after inoculation. The size <strong>of</strong> the lesions on each variety was compared using ANOVA and the<br />
results revealed that Huay Bong 60 showed the highest resistance to CAD whereas Hanatee was the most susceptible variety.<br />
The screening method developed in this study can be undertaken within a short time and without contamination <strong>of</strong> the<br />
pathogen into the environment. This methodology will be useful for identification <strong>of</strong> cassava varieties with resistance to CAD.<br />
© <strong>2010</strong> Australasian Plant Pathology Society.<br />
No.639<br />
Author (s) : Kuntawee, C., Fungladda, W., Kaewkungwal, J., Chanthavanich, P., Chotpittayasunon, T.<br />
Title : Social factors related to quality <strong>of</strong> life among HIV infected children in Ubon Ratchathani province, Thailand<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 5, September <strong>2010</strong>, Pages 1136-1144<br />
Document Type : Article<br />
Keywords : Children; HIV; Quality <strong>of</strong> life; Social factors; Thailand<br />
<strong>Abstract</strong> : A cross-sectional study was conducted to determine the social factors and quality <strong>of</strong> life <strong>of</strong> HIV infected children attending<br />
the Pediatric Infectious Disease Clinic, Sappasithiprasong Hospital, Ubon Ratchathani Province, Thailand. Data were collected<br />
during October-November 2008, by interviewing caretakers and their children using a structured questionnaire. The children's<br />
families were in need <strong>of</strong> improved social support (84.5%), since community resources provided limited support, such as<br />
clothes, food, financial support, consultation, and information. The HIV infected children's quality <strong>of</strong> life needed improvement<br />
(78.7%). The factors associated with quality <strong>of</strong> life included having others as main caretakers (OR 4.64, 95%CI 1.45-14.78),<br />
233
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
parental death (OR 4.19, 95%CI 1.55-11.31), age <strong>of</strong> caregivers above 45 years old (OR 9.52, 95%CI 2.62-34.53), and family<br />
income less than THB 5,000 per month (OR 5.25, 95%CI 1.14-23.39). However, on multivariate analysis, only age <strong>of</strong> caregiver<br />
was a significant predictor for quality <strong>of</strong> life <strong>of</strong> the child. Children who were cared for by caregivers aged 45 years or above<br />
had a better quality <strong>of</strong> life than those whose caregivers were 20-45 years old (OR 6.32, 95%CI 1.12-35.62). Therefore, to<br />
improve quality <strong>of</strong> life among HIV infected children, age <strong>of</strong> caregiver is an important factor to be considered. Government and<br />
non-government organizations should focus on supporting caregivers in terms <strong>of</strong> food, financial, and emotional support based<br />
on resources available in the community.<br />
No.640<br />
Author (s) : Kuptniratsaikul, V., Thanakhumtorn, S., Chinswangwatanakul, P., Wattanamongkonsil, L., Thamlikitkul, V.<br />
Title : Are Curcuma domestica (turmeric) extracts equally effective as ibupr<strong>of</strong>en for knee osteoarthritis?<br />
Source : Focus on Alternative and Complementary Therapies, Volume 15, Issue 2, June <strong>2010</strong>, Pages 117-118<br />
Document Type : Note<br />
Keywords : Curcuma domestica extract, glycosaminoglycan, ibupr<strong>of</strong>en, nonsteroid antiinflammatory agent, plant extract, unclassified drug;<br />
adult, clinical trial, controlled clinical trial, controlled study, drug efficacy, drug safety, drug withdrawal, human, knee<br />
osteoarthritis, major clinical study, note, outcome assessment, pain assessment, patient satisfaction, randomized controlled<br />
trial, rheumatology, single blind procedure, Thailand, treatment outcome, walking<br />
<strong>Abstract</strong> : –<br />
No.641<br />
Author (s) : Kusolsuk, T., Kamonrattanakun, S., Wesanonthawech, A., Dekumyoy, P., Thaenkham, U., Yoonuan, T., Nuamtanong, S., Sanguankiat,<br />
S., Pubampen, S., Maipanich, W., Panitchakit, J., Marucci, G., Pozio, E., Waikagul, J.<br />
Title : The second outbreak <strong>of</strong> trichinellosis caused by Trichinella papuae in Thailand<br />
Source : Transactions <strong>of</strong> the Royal Society <strong>of</strong> Tropical Medicine and Hygiene, Volume 104, Issue 6, June <strong>2010</strong>, Pages 433-437<br />
Document Type : Article<br />
Keywords : Outbreak; Pork; Thailand; Trichinella papuae; Trichinellosis; Wild pig<br />
<strong>Abstract</strong> : A human trichinellosis outbreak caused by Trichinella papuae occurred in the Uthai Thani Province <strong>of</strong> Thailand in September<br />
2007. A total <strong>of</strong> 34 villagers suffering at least one <strong>of</strong> the symptoms suggestive <strong>of</strong> trichinellosis, or those who were<br />
asymptomatic but had a history <strong>of</strong> ingesting raw wild pig meat, were enrolled in the study. Twenty-two villagers had ingested<br />
undercooked pork from a hunted wild pig (Sus scr<strong>of</strong>a). One patient with a severe clinical picture was hospitalised and more<br />
than 80 non-encapsulated larvae were detected in the muscle biopsy. The larvae were identified as T. papuae by molecular<br />
analyses <strong>of</strong> the mitochondrial cytochrome c oxidase subunit I (COI) gene and the expansion segment 5 (ES5) <strong>of</strong> the large<br />
subunit rRNA. Of the 34 suspected cases, 27 agreed to be subjected to haematological and serological tests. Immunoblot<br />
analysis using crude antigens from T. spiralis muscle larvae revealed anti- Trichinella IgG in 20 <strong>of</strong> the 26 serum samples (1<br />
serum sample could not be analysed). All infected people were successfully treated with mebendazole; the one patient with<br />
severe symptomatology was treated successfully with prednisolone. © 2009 Royal Society <strong>of</strong> Tropical Medicine and Hygiene.<br />
No.642<br />
Author (s) : Kutintara, B., Somboon, P., Chaengsri, S., Suanlim, S., Phankong, K., Chumnanprai, T., Sonsiri, W., Wongma, A.<br />
Title : Accessible market: A prototype for people with disabilities in Thailand<br />
Source : Disability and Rehabilitation: Assistive Technology, Volume 5, Issue 4, July <strong>2010</strong>, Pages 240-246<br />
Document Type : Article<br />
Keywords : Accessibility; Food stall; Market; People with disabilities<br />
<strong>Abstract</strong> : Purpose.Most Thai people like to purchase food at local markets; however, the accessibility to local markets by people with<br />
disabilities may be limited. The objective <strong>of</strong> this work was to develop a prototype <strong>of</strong> an accessible market for people with<br />
disabilities in Thailand, by performing a case study on a selected market in which people with disabilities <strong>of</strong>ten go. Methods.<br />
Representative players participated in this study: a market owner, food stall owners, assistive technology specialists, general<br />
customers, and customers with disabilities. Data were collected by observing environmental features in the market, and by<br />
interviewing the participants. Results.The results indicated that people with disabilities could not access the market because<br />
<strong>of</strong> lack <strong>of</strong> suitable parking zone, lack <strong>of</strong> ramps, walkways with obstacles, unsuitable public restrooms, the lack <strong>of</strong> zoned area<br />
for the food court and <strong>of</strong> standards for food stalls. Conclusion.After analysis <strong>of</strong> the data, a prototype was designed for parking<br />
spaces, ramps, walkways, public restroom, food court, and food stalls to make the market accessible. © <strong>2010</strong> Informa UK Ltd.<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.643<br />
Author (s) : Kypraios, T., O'Neill, P.D., Huang, S.S., Rifas-Shiman, S.L., Cooper, B.S.<br />
Title : Assessing the role <strong>of</strong> undetected colonization and isolation precautions in reducing Methicillin-Resistant Staphylococcus<br />
aureus transmission in intensive care units<br />
Source : BMC Infectious Diseases, Volume 10, 16 February <strong>2010</strong>, Article number 29<br />
Document Type : Article<br />
Keywords : article, bacterial colonization, bacterial transmission, bacterium detection, Bayes theorem, controlled study, health<br />
survey, human, infection control, intensive care unit, major clinical study, methicillin resistant Staphylococcus<br />
aureus, nonhuman, nose smear, patient care, protective clothing, screening test, adult, cross infection, disease<br />
transmission, heterozygote, isolation and purification, microbiology, nose, Staphylococcus infection; Adult, Carrier State, Cross<br />
Infection, Disease Transmission, Infectious, Humans, Intensive Care Units, Methicillin-Resistant Staphylococcus<br />
aureus, Nose, Patient Isolation, Staphylococcal Infections<br />
<strong>Abstract</strong> : Background: Screening and isolation are central components <strong>of</strong> hospital methicillin-resistant Staphylococcus aureus (MRSA)<br />
control policies. Their prevention <strong>of</strong> patient-to-patient spread depends on minimizing undetected and unisolated MRSApositive<br />
patient days. Estimating these MRSA-positive patient days and the reduction in transmission due to isolation presents<br />
a major methodological challenge, but is essential for assessing both the value <strong>of</strong> existing control policies and the potential<br />
benefit <strong>of</strong> new rapid MRSA detection technologies. Recent methodological developments have made it possible to estimate<br />
these quantities using routine surveillance data.Methods: Colonization data from admission and weekly nares cultures were<br />
collected from eight single-bed adult intensive care units (ICUs) over 17 months. Detected MRSA-positive patients were<br />
isolated using single rooms and barrier precautions. Data were analyzed using stochastic transmission models and model<br />
fitting was performed within a Bayesian framework using a Markov chain Monte Carlo algorithm, imputing unobserved MRSA<br />
carriage events.Results: Models estimated the mean percent <strong>of</strong> colonized-patient-days attributed to undetected carriers as<br />
14.1% (95% CI (11.7, 16.5)) averaged across ICUs. The percent <strong>of</strong> colonized-patient-days attributed to patients awaiting results<br />
averaged 7.8% (6.2, 9.2). Overall, the ratio <strong>of</strong> estimated transmission rates from unisolated MRSA-positive patients and those<br />
under barrier precautions was 1.34 (0.45, 3.97), but varied widely across ICUs.Conclusions: Screening consistently detected<br />
>80% <strong>of</strong> colonized-patient-days. Estimates <strong>of</strong> the effectiveness <strong>of</strong> barrier precautions showed considerable uncertainty, but in<br />
all units except burns/general surgery and one cardiac surgery ICU, the best estimates were consistent with reductions in<br />
transmission associated with barrier precautions. © <strong>2010</strong> Kypraios et al; licensee BioMed Central Ltd.<br />
No.644<br />
Author (s) : Lai, S., Wiwatanapataphee, B.<br />
Title : The well-posedness <strong>of</strong> the global solution for a damped euler-bernoulli equation<br />
Source : <strong>International</strong> Journal <strong>of</strong> Pure and Applied Mathematics, Volume 59, Issue 2, <strong>2010</strong>, Pages 203-212<br />
Document Type : Article<br />
Keywords : Beam equation; Global solution; Initial value problem<br />
<strong>Abstract</strong> : In this paper, we study the well-posedness <strong>of</strong> the global solution to the following damped Euler-Bernoulli equation utt +<br />
auxxxx + 2but + cu = f(u), t≥0, x; ε [0, + ∞). For the case f(u) = u2, the existence and uniqueness <strong>of</strong> the global solution to an<br />
initial value problem <strong>of</strong> the equation are established in the space C([0,+∞),L2([0,+∞)))∩C1([0,+∞), H-1([0,+∞))). For the case<br />
where f(u) is a polynomial, we find that the well-posedness can be established in the Sobolev space C([0, +∞), Hs([0, +∞)))<br />
∩ C1([0, +∞), H s-1([0, +∞))) (s > 1/2). © <strong>2010</strong> Academic <strong>Publications</strong>.<br />
No.645<br />
Author (s) : Laksameethanasan, D., Brandt, S.S.<br />
Title : A bayesian reconstruction method with marginalized uncertainty model for camera motion in microrotation imaging<br />
Source : IEEE Transactions on Biomedical Engineering, Volume 57, Issue 7, July <strong>2010</strong>, Article number 5422680, Pages 1719-1728<br />
Document Type : Article<br />
Keywords : Cell biology; cellmanipulation; image reconstruction; light microscopy; microrotation imaging; statistical inverse problems<br />
<strong>Abstract</strong> : Reconstruction <strong>of</strong> a 3-D structure from multiple projection images requires prior knowledge <strong>of</strong> projection directions or camera<br />
motion parameters that describe the relative positions and orientations <strong>of</strong> 3-D structure with respect to the camera. These<br />
parameters can be estimated using, for instance, the conventional correlation alignment and feature-based methods.<br />
However, the alignment methods are not perfect, where the inaccuracy <strong>of</strong> the estimated motion parameters causes artifacts<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
in the reconstruction. To overcome this problem, we propose a Bayesian approach to reconstruct the object that takes the<br />
motion uncertainty distribution into account. Moreover, we consider the motion parameters as nuisance parameters and<br />
integrate them out from the posterior distribution, assuming a Gaussian uncertainty model, which yields a statistical cost<br />
function to be minimized. The proposed method is applied in microrotation fluorescence imaging, where we aim at 3-D<br />
reconstruction <strong>of</strong> a rotating object from an image series, acquired by an optical microscope. The experiments with simulated<br />
and real microrotation datasets demonstrate that the proposed method provides visually and numerically better results than<br />
the traditional reconstruction methods, which ignore the uncertainty <strong>of</strong> the motion estimates. © 2006 IEEE.<br />
No.646<br />
Author (s) : Lallemant, M., Ngo-Giang-Huong, N., Jourdain, G., Traisaithit, P., Cressey, TR., Collins, IJ., Jarupanich, T., Sukhumanant, T.,<br />
Achalapong, J., Sabsanong, P., Chotivanich, N., Winiyakul, N., Ariyadej, S., Kanjanasing, A., Ratanakosol, J., Hemvuttiphan, J.,<br />
Kengsakul, K., Wannapira, W., Sittipiyasakul, V., Pornkitprasarn, W., Liampongsabuddhi, P., McIntosh, K., Van Dyke, RB., Frenkel,<br />
LM., Koetsawang, S., Le Coeur, S., Kanchana, S.<br />
Title : Efficacy and Safety <strong>of</strong> 1-Month Postpartum Zidovudine-Didanosine to Prevent HIV-Resistance Mutations after Intrapartum<br />
Single-Dose Nevirapine<br />
Source : CLINICAL INFECTIOUS DISEASES, Volume: 50, Issue: 6, Pages: 898-908, DOI: 10.1086/650745, Published: MAR 15 <strong>2010</strong><br />
Document Type : Article<br />
Keywords : TO-CHILD TRANSMISSION; ACTIVE ANTIRETROVIRAL THERAPY; HEPATITIS-B; VERTICAL TRANSMISSION; RECEIVING NEVIRAPINE;<br />
RANDOMIZED-TRIAL; SUBTYPE-A; WOMEN; EXPOSURE; DISCONTINUATION<br />
<strong>Abstract</strong> : Background. Intrapartum single-dose nevirapine plus third trimester maternal and infant zidovudine are essential components<br />
<strong>of</strong> programs to prevent mother-to-child transmission <strong>of</strong> human immunodeficiency virus (HIV) in resource-limited settings. The<br />
persistence <strong>of</strong> nevirapine in the plasma for 3 weeks postpartum risks selection <strong>of</strong> resistance mutations to nonnucleoside<br />
reverse-transcriptase inhibitors (NNRTIs). We hypothesized that a 1-month zidovudine-didanosine course initiated at the same<br />
time as single-dose nevirapine (sdNVP) would prevent the selection <strong>of</strong> nevirapine-resistance mutations. Methods. HIV-infected<br />
pregnant women in the PHPT-4 cohort with CD4 cell counts >250 cells/mm(3) received antepartum zidovudine from the third<br />
trimester until delivery, sdNVP during labor, and a 1-month zidovudine-didanosine course after delivery. These women were<br />
matched on the basis <strong>of</strong> baseline HIV load, CD4 cell count, and duration <strong>of</strong> antepartum zidovudine to women who received<br />
sdNVP in the PHPT-2 trial (control subjects). Consensus sequencing and the more sensitive oligonucleotide ligation assay were<br />
performed on samples obtained on postpartum days 7-10, 37-45, and 120 (if the HIV load was >500 copies/mL) to detect<br />
K103N/Y181C/G190A mutations. Results. The 222 PHPT-4 subjects did not differ from matched control subjects in baseline<br />
characteristics except for age. The combined group median CD4 cell count was 421 cells/mm(3) (interquartile range [IQR],<br />
322-549 cells/mm3), the median HIV load was 3.45 log(10) copies/mL (IQR, 2.79-4.00 log(10) copies/mL), and the median<br />
duration <strong>of</strong> zidovudine prophylaxis was 10.4 weeks (IQR, 9.1-11.4 weeks). Using consensus sequencing, major NNRTI resistance<br />
mutations were detected after delivery in 0% <strong>of</strong> PHPT-4 subjects and 10.4% <strong>of</strong> PHPT-2 controls. The oligonucleotide ligation<br />
assay detected resistance in 1.8% <strong>of</strong> PHPT-4 subjects and 18.9% <strong>of</strong> controls. Major NNRTI resistance mutations were detected<br />
by either method in 1.8% <strong>of</strong> PHPT-4 subjects and 20.7% <strong>of</strong> controls (P
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Protective effect <strong>of</strong> makrut lime leaf (Citrus hystrix) in HepG2 cells: Implications for oxidative stress<br />
Source : ScienceAsia, Volume 36, Issue 2, June <strong>2010</strong>, Pages 112-117<br />
Document Type : Article<br />
Keywords : Antioxidant enzyme; Hydroxyl radical; Lipid peroxidation; Phenolics<br />
<strong>Abstract</strong> : The objective <strong>of</strong> the present study was to examine fresh and processed (boiled or fried) leaves <strong>of</strong> Citrus hystrix in terms <strong>of</strong><br />
total phenolic content, malondialdehyde content, 1,1-diphenyl-2-picrylhydrazyl (DPPH), and hydroxyl radicals scavenging<br />
activities, the baseline levels <strong>of</strong> glutathione S-transferase (GST), superoxide dismutase (SOD), and catalase in HepG2 cells. The<br />
results indicated that fresh C. hystrix leaves possess the higher hydroxyl radical scavenging activity (22 mg/ml). Pretreatment<br />
<strong>of</strong> C. hystrix leaf extracts decreases GST, SOD, or catalase activity induced by H2O2, rendering them unsuitable for cancer<br />
chemoprevention, superoxide scavenging, or hydrogen peroxide detoxification. A linear relation between the total phenolic<br />
content and DPPH or hydroxyl radical activities <strong>of</strong> the extract was not observed. This study showed that C. hystrix leaf may<br />
exert its antioxidative stress by scavenging hydroxyl radicals and inhibiting lipid peroxidation that causes oxidative damage to<br />
HepG2 cells.<br />
No.649<br />
Author (s) : Laosiripojana, N., Charojrochkul, S., Kim-Lohsoontorn, P., Assabumrungrat, S.<br />
Title : Role and advantages <strong>of</strong> H2S in catalytic steam reforming over nanoscale CeO2-based catalysts<br />
Source : Journal <strong>of</strong> Catalysis, Volume 276, Issue 1, 19 November <strong>2010</strong>, Pages 6-15<br />
Document Type : Article<br />
Keywords : CeO2; H2S; Hydrogen; Steam reforming<br />
<strong>Abstract</strong> : The activity <strong>of</strong> nanoscale CeO2 and doped CeO2 (with Gd, Y, Nb, La, and Sm) toward the steam reforming <strong>of</strong> CH4 in the<br />
presence <strong>of</strong> H2S was investigated for later application as an in-stack reforming catalyst in a solid oxide fuel cell. Although H2S<br />
is commonly known as a poisonous gas for metallic-based catalysts, it was found that the presence <strong>of</strong> appropriate H2S<br />
content increases the reforming activity <strong>of</strong> these CeO2-based catalysts. According to postreaction catalyst characterizations by<br />
X-ray diffraction, X-ray photoelectron spectroscopy, temperature-programmed reduction, temperature-programmed<br />
desorption, H2/H2O + H2S titration, and 18O/16O isotope exchange, it was revealed that this behavior is related to the<br />
formation <strong>of</strong> various Ce-O-S phases (Ce(SO 4)2, Ce2(SO4)3, and Ce2O2S) during the reaction. Our studies indicated that the<br />
formation <strong>of</strong> Ce(SO4)2 promotes the oxygen storage capacity, the lattice oxygen mobility, and eventually the reforming<br />
activity, whereas the formation <strong>of</strong> Ce2O2S oppositely reduces both properties and lowers the reforming rate. © <strong>2010</strong> Elsevier<br />
Inc. All rights reserved.<br />
No.650<br />
Author (s) : Laosiripojana, N., Sutthisripok, W., Kim-Lohsoontorn, P., Assabumrungrat, S.<br />
Title : Reactivity <strong>of</strong> Ce-ZrO2 (doped with La-, Gd-, Nb-, and Sm-) toward partial oxidation <strong>of</strong> liquefied petroleum gas: Its application<br />
for sequential partial oxidation/steam reforming<br />
Source : <strong>International</strong> Journal <strong>of</strong> Hydrogen Energy, Volume 35, Issue 13, July <strong>2010</strong>, Pages 6747-6756<br />
Document Type : Article<br />
Keywords : Ce-ZrO2; Hydrogen; Liquefied petroleum gas; Partial oxidation<br />
<strong>Abstract</strong> : Ce-ZrO2 was found to have useful partial oxidation activity under moderate temperatures. It converted liquefied petroleum<br />
gas (LPG) to H2, CH4, CO and CO2 with small amounts <strong>of</strong> C2H6 and C2H4 formations depending on the operating temperature<br />
and provided significantly greater resistance toward carbon deposition compared to conventional Ni/Al2O 3. The doping <strong>of</strong> La,<br />
Sm and Gd over Ce-ZrO2 considerably improved catalytic reactivity, whereas Nb-doping reduced its reactivity. It was found<br />
that the impact <strong>of</strong> doping element is strongly related to the degrees <strong>of</strong> oxygen storage capacity (OSC) and/or lattice oxygen<br />
(OOx) <strong>of</strong> materials. Among all catalysts, La-doped Ce-ZrO2 was observed to have highest OSC value and was the most active<br />
catalyst. Above 850 °C with inlet LPG/O2 molar ratio <strong>of</strong> 1.0/1.0, the main products from the reaction over La-doped Ce-ZrO2<br />
were H2, CH4, CO, and CO2. Practical application was then proposed by applying La-doped Ce-ZrO2 as primary oxidative<br />
catalyst for sequential partial oxidation/steam reforming <strong>of</strong> LPG (by using Ni/Al2O3 as the steam reforming catalyst). At 850 °C,<br />
this coupling pattern <strong>of</strong>fered high H2 yield (87.0-91.4%) without any hydrocarbons left in the products indicating the<br />
complete conversion <strong>of</strong> LPG to syngas. H2 yield from this system was almost identical to that observed from the typical<br />
autothermal reforming over Rh/Al2O3; hence it could efficiently replace the requirement <strong>of</strong> expensive noble metal catalysts<br />
to reform LPG properly. © <strong>2010</strong> Pr<strong>of</strong>essor T. Nejat Veziroglu. Published by Elsevier Ltd. All rights reserved.<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.651<br />
Author (s) : Laosombat, V., Viprakasit, V., Dissaneevate, S., Leetanaporn, R., Chotsampancharoen, T., Wongchanchailert, M., Kodchawan, S.,<br />
Thongnoppakun, W., Duangchu, S.<br />
Title : Natural history <strong>of</strong> Southeast Asian Ovalocytosis during the first 3years <strong>of</strong> life<br />
Source : Blood Cells, Molecules, and Diseases, Volume 45, Issue 1, June <strong>2010</strong>, Pages 29-32<br />
Document Type : Article<br />
Keywords : AE-1 gene; Anemia; Neonatal hyperbilirubinemia; Southeast Asian Ovalocytosis (SAO)<br />
<strong>Abstract</strong> : Southeast Asian Ovalocytosis (SAO), the most common red cell membrane disorder found in the Far-East and Pacific rim,<br />
appears to be innocuous in man since it has been identified mostly in non-anemic healthy individuals. To further substantiate<br />
our previous observation that this condition might be symptomatic particularly in the neonatal period, we studied 1567<br />
newborns from Southern Thailand where SAO is prevalent. Thirty-one babies (1: 50 with allele frequency <strong>of</strong> 0.01) have been<br />
identified with SAO and confirmed molecularly to carry a single defective AE-1 (band 3) allele. These babies had significant<br />
anemia at birth due to hemolysis with 51.6% <strong>of</strong> them developing neonatal hyperbilirubinemia. Co-inheritance <strong>of</strong> common<br />
UGT1A1 variants in such cases was not associated with their degree <strong>of</strong> jaundice. Interestingly, hematology data <strong>of</strong> these SAO<br />
babies became "normalized" in the first 3. years <strong>of</strong> life without further evidence <strong>of</strong> on-going and/or even "compensated"<br />
hemolysis. © <strong>2010</strong>.<br />
No.652<br />
Author (s) : Laosutthipong, C., Flegel, T.<br />
Title : Viprolaxikine, a Novel Cytokine-like Protein from Insect Cell Cultures can Reduce Dengue-2 Virus Titres in Mammalian Cells<br />
Source : ANTIVIRAL RESEARCH, Volume: 86, Issue: 1, Pages: A36-A37, DOI: 10.1016/j.antivira1.<strong>2010</strong>.02.383, Meeting <strong>Abstract</strong>: 73,<br />
Published: APR <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.653<br />
Author (s) : Laothavorn, P., Hengrussamee, K., Kanjanavanit, R., Moleerergpoom, W., Laorakpongse, D., Pachirat, O., Boonyaratavej, S.,<br />
Sritara, P.<br />
Title : Thai acute decompensated heart failure registry (Thai ADHERE)<br />
Source : CVD Prevention and Control, Volume 5, Issue 3, September <strong>2010</strong>, Pages 89-95<br />
Document Type : Article<br />
Keywords : Heart failure; Neurohormonal blockers; Thai ADHERE registry; Thai heart failure registry<br />
<strong>Abstract</strong> : Background: Heart Failure (HF) is the one <strong>of</strong> the malignant cardiac syndromes which has a high morbidity and mortality rate.<br />
In Thailand, HF is one <strong>of</strong> the major cardiovascular health problems and economic burdens disease. Thai ADHERE is the first HF<br />
registry in Thailand. Objective: To assess patient with HF in Thailand in terms <strong>of</strong> patients' characteristics, clinical presentation,<br />
causes <strong>of</strong> heart failure, and precipitating causes <strong>of</strong> heart failure, hospital course, management, and in-hospital outcomes.<br />
Material and methods: Thai Acute Decompensated Heart Failure Registry or Thai ADHERE registry is a Phase IV, multicenter,<br />
observational, and open-label registry in 18 cardiac centers in Thailand using the US ADHERE protocol. Medical records <strong>of</strong><br />
hospitalized patients with the principal discharge diagnosis <strong>of</strong> HF from March 2006 to November 2007 were validated and<br />
registered via an electronic web based system. Results: There were 2041 HF admissions in 1612 patients with a median age <strong>of</strong><br />
67 years (mean 64 ± 14 years). Age >75 years was found in 24%, 49.6% were male patients, and 67% <strong>of</strong> these admissions had<br />
prior heart failure. Underlying diseases were hypertension [(HT) = 65%], coronary artery disease [(CAD) = 47%], dyslipidemia<br />
(50%), diabetes mellitus (47%), atrial fibrillation (24%) and chronic kidney disease (19%). Clinical features at presentation were<br />
dyspnea (97%), edema (60%), pulmonary rales (85%) and pulmonary congestion by chest X-ray (93%). Sixty-nine percent<br />
(69%) were in NYHA functional class IV and 44% had a left ventricular ejection fraction (LVEF) less than 40%. Common causes<br />
<strong>of</strong> heart failure were CAD (45%), valvular heart diseases (19%), cardiomyopathy (14%) and HT (12%). Precipitating causes <strong>of</strong><br />
heart failure were heart disease itself 54% and 20% were related to inadequate diuretics and poor patient compliance with<br />
medications. Neurohormonal blockers (NHB) [angiotensin converting enzyme inhibitors (ACEI), angiotensinoge receptor blocker<br />
(ARB), aldosterone blocker (AA) and beta blockers (BB)], were given prior to hospitalization in comparison to at discharge in<br />
26% vs. 35%, 12% vs. 12%, 13% vs. 17% and 26% vs. 24% respectively. In-hospital mortality rate was 5.5% and the median<br />
length <strong>of</strong> hospitalization was 7.5 days. Major causes <strong>of</strong> death were sepsis, worsening <strong>of</strong> heart failure, arrhythmic arrest and<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
acute coronary syndrome. There was a higher mortality rate in those with poor LV systolic function compared to those with<br />
preserved LV systolic function (8.2% vs. 4.1%; p = .008). At discharge, 23% <strong>of</strong> the patients were asymptomatic while 69% had<br />
symptomatic improvement. Conclusion: Thai ADHERE registry revealed that Thai patients hospitalized for heart failure are<br />
younger and sicker than European and American patients. There is a high prevalence <strong>of</strong> HF with preserved ejection fraction.<br />
CAD was the most common cause <strong>of</strong> HF while HT was the most common underlying disease. There was a 5.4% mortality<br />
rate, which was higher in those with poor LV systolic function. There was underutilization <strong>of</strong> NHB (ACEI, ARB, BB and AA). ©<br />
<strong>2010</strong> Published by Elsevier Ltd. on behalf <strong>of</strong> World Heart Federation.<br />
No.654<br />
Author (s) : Larbcharoensub, N., Wongwichai, S., Chongtrakool, P., Boongird, A., Noinang, A., Watcharananan, S.P., Tunlayadechanont, S.,<br />
Witoonpanich, R., Phudhichareonrat, S.<br />
Title : Cerebral aspergillosis and cerebral candidiasis; a retrospective analysis <strong>of</strong> clinicopathologic features in ramathibodi hospital<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 12, December <strong>2010</strong>, Pages 1443-1450<br />
Document Type : Article<br />
Keywords : Aspergillosis; Brain abscess; Candidiasis; Cerebral mycosis; Fungus<br />
<strong>Abstract</strong> : Objective: Determine and compare the clinicopathological findings <strong>of</strong> cerebral aspergillosis with cerebral candidiasis. Material<br />
and Method: The medical records with cerebral aspergillosis and cerebral candidiasis in Ramathibodi Hospital between<br />
January 1997 and December 2008 were analyzed. The criterion for the diagnosis <strong>of</strong> cerebral aspergillosis and cerebral<br />
candidiasis was the evidence <strong>of</strong> fungal elements from histopathologic section. The age, gender, neurological manifestations,<br />
duration <strong>of</strong> symptom, associated underlying disease, predisposing risk factor, laboratory data, extent <strong>of</strong> systemic organ<br />
involvement and treatment outcome were analyzed. Results: The present study included cerebral aspergillosis (n = 41) and<br />
candidiasis (n = 15). There were 23 male and 33 female patients. The mean and median ages at diagnosis were 39.7 and 45<br />
years, respectively (range, 1 month to 87 years). The clinical presentations included alteration <strong>of</strong> consciousness (69.6%), fever<br />
(60.7%), weakness <strong>of</strong> the extremity (14.3%), cranial nerve palsy (12.5%), headache (12.59%) and seizure (5.4%). One third <strong>of</strong><br />
the cases had underlying ematologic malignancy. The cerebral aspergillosis and cerebral candidiasis were associated with<br />
corticosteroids treatment in 32.1%. The frequent associated sites <strong>of</strong> fungal infection included the lungs (73.2%), alimentary<br />
tract (33.9%) and sinonasal tract (19.6%). Conclusion: A diagnosis <strong>of</strong> cerebral aspergillosis and cerebral candidiasis requires a<br />
high index <strong>of</strong> suspicion especially in immunocompromised patients who presented with alteration <strong>of</strong> consciousness, fever,<br />
focal neurological deficit, headache, and seizure. The patients with cerebral aspergillosis and cerebral candidiasis manifest<br />
with similar clinicopathologic features. However, the sinonasal tract infection and abscess formation are more common in<br />
cerebral aspergillosis. Associated alimentary tract infection is commonly seen in cerebral candidiasis.<br />
No.655<br />
Author (s) : Law, M.J., Lower, K.M., Voon, H.P.J., Hughes, J.R., Garrick, D., Viprakasit, V., Mitson, M., De Gobbi, M., Marra, M., Morris, A.,<br />
Abbott, A., Wilder, S.P., Taylor, S., Santos, G.M., Cross, J., Ayyub, H., Jones, S., Ragoussis, J., Rhodes, D., Dunham, I., Higgs, D.R.,<br />
Gibbons, R.J.<br />
Title : ATR-X syndrome protein targets tandem repeats and influences allele-specific expression in a size-dependent manner<br />
Source : Cell, Volume 143, Issue 3, 29 October <strong>2010</strong>, Pages 367-378<br />
Document Type : Article<br />
Keywords : DNA; HUMDISEASE; PROTEINS<br />
<strong>Abstract</strong> : ATRX is an X-linked gene <strong>of</strong> the SWI/SNF family, mutations in which cause syndromal mental retardation and downregulation<br />
<strong>of</strong> α-globin expression. Here we show that ATRX binds to tandem repeat (TR) sequences in both telomeres and euchromatin.<br />
Genes associated with these TRs can be dysregulated when ATRX is mutated, and the change in expression is determined by<br />
the size <strong>of</strong> the TR, producing skewed allelic expression. This reveals the characteristics <strong>of</strong> the affected genes, explains the<br />
variable phenotypes seen with identical ATRX mutations, and illustrates a new mechanism underlying variable penetrance.<br />
Many <strong>of</strong> the TRs are G rich and predicted to form non-B DNA structures (including G-quadruplex) in vivo. We show that ATRX<br />
binds G-quadruplex structures in vitro, suggesting a mechanism by which ATRX may play a role in various nuclear processes<br />
and how this is perturbed when ATRX is mutated. © <strong>2010</strong> Elsevier Inc.<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.656<br />
Author (s) : Lawaly, Y.R., kuntabhai, A., Marrama, L., Konate, L., Phimpraphi, W., Sokhna, C., Tall, A., Sarr, F.D., Peerapittayamongkol, C.,<br />
Louicharoen, C., Schneider, B.S., Levescot, A., Talman, A., Casademont, I., Menard, D., Trape, J., Rogier, C., Kaewkunwal, J.,<br />
Sura, T., Nuchprayoon, I., Ariey, F., Baril, L., Singhasivanon, P., Mercereau-Puijalon, O., Paul, R.<br />
Title : Heritability <strong>of</strong> the human infectious reservoir <strong>of</strong> malaria parasites<br />
Source : PLoS ONE, Volume 5, Issue 6, <strong>2010</strong>, Article number e11358<br />
Document Type : Article<br />
Keywords : alpha globin, hemoglobin S; adolescent, adult, article, blood smear, child, disease carrier, family study, female, follow<br />
up, gametocyte, gene mutation, genetic association, Giemsa stain, heritability, human, human genetics, infant, infection<br />
resistance, infection sensitivity, longitudinal study, major clinical study, malaria, male, nonhuman, parasite<br />
transmission, Plasmodium falciparum, Plasmodium vivax, preschool child, prevalence, restriction fragment length<br />
polymorphism, reverse transcription polymerase chain reaction, school child, Senegal, symptom, Thailand, alpha<br />
thalassemia, animal, cohort analysis, parasitology, pathogenicity, sickle cell anemia; Plasmodium falciparum; alpha-<br />
Thalassemia, Anemia, Sickle Cell, Animals, Cohort Studies, Disease Reservoirs, Humans, Plasmodium falciparum, Plasmodium<br />
vivax, Reverse Transcriptase Polymerase Chain Reaction<br />
<strong>Abstract</strong> : Background: Studies on human genetic factors associated with malaria have hitherto concentrated on their role in<br />
susceptibility to and protection from disease. In contrast, virtually no attention has been paid to the role <strong>of</strong> human genetics<br />
in eliciting the production <strong>of</strong> parasite transmission stages, the gametocytes, and thus enhancing the spread <strong>of</strong> disease.<br />
Methods and Findings: We analysed four longitudinal family-based cohort studies from Senegal and Thailand followed for 2-8<br />
years and evaluated the relative impact <strong>of</strong> the human genetic and non-genetic factors on gametocyte production in<br />
infections <strong>of</strong> Plasmodium falciparum or P. vivax. Prevalence and density <strong>of</strong> gametocyte carriage were evaluated in<br />
asymptomatic and symptomatic infections by examination <strong>of</strong> Giemsa-stained blood smears and/or RT-PCR (for falciparum in<br />
one site). A significant human genetic contribution was found to be associated with gametocyte prevalence in asymptomatic<br />
P. falciparum infections. By contrast, there was no heritability associated with the production <strong>of</strong> gametocytes for P. falciparum<br />
or P. vivax symptomatic infections. Sickle cell mutation, HbS, was associated with increased gametocyte prevalence but its<br />
contribution was small. Conclusions: The existence <strong>of</strong> a significant human genetic contribution to gametocyte prevalence in<br />
asymptomatic infections suggests that candidate gene and genome wide association approaches may be usefully applied to<br />
explore the underlying human genetics. Prospective epidemiological studies will provide an opportunity to generate novel<br />
and perhaps more epidemiologically pertinent gametocyte data with which similar analyses can be performed and the role <strong>of</strong><br />
human genetics in parasite transmission ascertained. © Lawaly et al.<br />
No.657<br />
Author (s) : Lawpoolsri, S., Chavez, I.F.; Yimsamran, S., Puangsa-art, S., Thanyavanich, N., Maneeboonyang, W., Chaimungkun, W.,<br />
Singhasivanon, P., Maguire, J.H.; Hungerford, L.L.<br />
Title : The impact <strong>of</strong> human reservoir <strong>of</strong> malaria at a community-level on individual malaria occurrence in a low malaria<br />
transmission setting along the Thai-Myanmar border<br />
Source : MALARIA JOURNAL, Volume: 9, Article Number: 143, DOI: 10.1186/1475-2875-9-143, Published: MAY 26 <strong>2010</strong><br />
Document Type : Article<br />
Keywords : PLASMODIUM-FALCIPARUM; MEFLOQUINE-ARTESUNATE; SEASONAL TRANSMISSION; VIVAX-MALARIA; PRIMAQUINE; AREA;<br />
EPIDEMIOLOGY; ARTEMISININ; GAMETOCYTES; INFECTIONS<br />
<strong>Abstract</strong> : Background: The probability <strong>of</strong> contracting malaria in a given individual is determined not only by the individual's<br />
characteristics, but also the ecological factors that characterize the level <strong>of</strong> human-vector contact in the population.<br />
Examination <strong>of</strong> the relationship between "individual" and "supra-individual" variables over time is important for understanding<br />
the local malaria epidemiology. This is essential for planning effective intervention strategies specifically for each location.<br />
Methods: A retrospective cohort study was conducted, which followed a community-cohort <strong>of</strong> about 3,500 residents in seven<br />
hamlets along the Thai-Myanmar border between 1999 and 2006. Potential malaria determinants measured at different levels<br />
(temporal variables, individual variables, and hamlet variables) were incorporated into multilevel models to estimate their<br />
effects on an individual's risk <strong>of</strong> malaria attack. Results: The monthly minimum temperature was significantly associated with<br />
the seasonal variation <strong>of</strong> malaria risk. An individual risk <strong>of</strong> malaria attack decreased by about 50% during the period that<br />
active surveillance was conducted; an additional 15% and 25% reduction <strong>of</strong> Plasmodium falciparum and Plasmodium vivax<br />
incidence, respectively, was observed after the use <strong>of</strong> artesunate-mefloquine combination therapy (ACT) for treatment <strong>of</strong> P.<br />
falciparum. Male children (age < 16 years old) were at highest risk <strong>of</strong> both P. falciparum and P. vivax attack. An increase in the<br />
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hamlet's incidence <strong>of</strong> P. falciparum and P. vivax by 1 per 100 persons in a previous month resulted in 1.14 and 1.34 times<br />
increase in the risk <strong>of</strong> P. falciparum and P. vivax, respectively, among individuals in a particular hamlet. Conclusion: In a small<br />
area with low malaria transmission intensity, the variation in mosquito abundance is relatively similar across the residential<br />
areas; incidence <strong>of</strong> malaria between hamlets, which reflects the community level <strong>of</strong> human infectious reservoirs, is an<br />
important predictor for the malaria risk among individuals within these hamlets. Therefore, local malaria control strategies<br />
should focus on interventions that aim to reduce the gametocyte carriage in the population, such as early detection and<br />
treatment programmes and the use <strong>of</strong> ACT for P. falciparum.<br />
No.658<br />
Author (s) : Lawung, R., Charoenwatanachokchai, A., Cherdtrakulkiat, R., Thammapiwan, S., Mungniponpan, T., Bülow, L., Prachayasittikul,<br />
V.<br />
Title : Antibiograms and randomly amplified polymorphic DNA-polymerase chain reactions (RAPD-PCR) as epidemiological markers <strong>of</strong><br />
gonorrhea<br />
Source : Journal <strong>of</strong> Clinical Laboratory Analysis, Volume 24, Issue 1, <strong>2010</strong>, Pages 31-37<br />
Document Type : Article<br />
Keywords : Gonorrhea; Gyrase A; Neissseria gonorrhoeae; RAPD-PCR<br />
<strong>Abstract</strong> : The development <strong>of</strong> antimicrobial resistance <strong>of</strong> Neisseria gonorrhoeae arising from wide dissemination <strong>of</strong> resistant clones is a<br />
major global health problem. In this study, a total <strong>of</strong> 235 isolates <strong>of</strong> N. gonorrhoeae isolated from patients <strong>of</strong> Bangrak<br />
Hospital were tested for their antibiotic susceptibilities to penicillin, norfloxacin, <strong>of</strong>loxacin, cipr<strong>of</strong>loxacin, spectinomycin, and<br />
ceftriaxone. Mutation (Ser-91) in the quinolone resistance determining regions <strong>of</strong> gyrA and random amplification <strong>of</strong> the<br />
polymorphic DNA polymerase chain reaction (RAPD-PCR) were examined from 145 isolates. Among these, 55 isolates were<br />
obtained during January-March 2000, 46 isolates during January-March 2002, and 44 isolates during October-December 2002.<br />
The occurrence <strong>of</strong> combination resistance between penicillin and quinolone was 20% in January-March 2000, which was<br />
increased to 57.8% during the period <strong>of</strong> October-December 2002 (P
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : antineoplastic agent, bevacizumab, monoclonal antibody, paclitaxel; adjuvant therapy, breast tumor, case report, chemically<br />
induced disorder, drug effect, female, human, letter, mastectomy, middle aged, necrosis, pathology, plastic<br />
surgery, postoperative complication, skin, skin disease, wound healing; Antibodies, Monoclonal, Antineoplastic Combined<br />
Chemotherapy Protocols, Breast Neoplasms, Female, Humans, Mastectomy, Middle Aged, Necrosis, Neoadjuvant<br />
Therapy, Paclitaxel, Postoperative Complications, Skin, Skin Diseases, Surgical Flaps, Wound Healing<br />
<strong>Abstract</strong> : –<br />
No.661<br />
Author (s) : Leardkamolkarn, V., Sirigulpanit, W., Kinney, R.M.<br />
Title : Characterization <strong>of</strong> recombinant dengue-2 virus derived from a single nucleotide substitution in the 5 ′ noncoding region<br />
Source : Journal <strong>of</strong> Biomedicine and Biotechnology, Volume <strong>2010</strong>, <strong>2010</strong>, Article number 934694<br />
Document Type : Article<br />
Keywords : virus RNA, complementary DNA; Aedes aegypti, animal experiment, animal model, article, cell culture, controlled<br />
study, Dengue virus, Dengue virus 2, female, genetic stability, immunogenicity, life cycle, mosquito, nonhuman, parasite<br />
vector, phenotype, RNA structure, single nucleotide polymorphism, structure analysis, temperature sensitivity, viremia, virus<br />
characterization, virus recombinant, virus replication, virus titration, virus transcription, virus transmission, virus<br />
virulence, Aedes, animal, cell line, chemistry, conformation, genetics, Institute for Cancer Research<br />
mouse, mouse, physiology, point mutation, temperature, virology, virulence; Dengue virus type 2, Mus; Aedes, Animals, Cell<br />
Line, Dengue Virus, DNA, Complementary, Female, Mice, Mice, Inbred ICR, Nucleic Acid Conformation, Phenotype, Point<br />
Mutation, RNA, Viral, Temperature, Viremia, Virulence, Virus Replication<br />
<strong>Abstract</strong> : Variants <strong>of</strong> wild-type dengue serotype 2 (DEN-2) virus containing nucleotide substitutions at positions 14, 15, or 57 in the 5 ′<br />
NCR were constructed by PCR-mediated site-directed mutagenesis. All three viruses containing a single point substitution<br />
demonstrated attenuation phenotype as evidenced by decreases replication and plaque size in cell culture assay. All three<br />
variants were less neurovirulent in newborn mice compared to the wild type. The mutants were immunogenic in adult mice<br />
immunogenicity and maintained stable replication characteristics following passage in mice. The variant viruses were<br />
competent for replication in Aedes aegypi mosquito vector, albeit at lower levels <strong>of</strong> infection and dissemination in the<br />
mosquito than the wild-type Den-2 16681 virus. Although all <strong>of</strong> the viruses, including the wild type, were found transmissible<br />
in mosquito life cycles, they were found subsequentially decreased in efficiency <strong>of</strong> infection, transmission, and dissemination<br />
rates along the mosquito generations and all <strong>of</strong> them remained genetically stable. © <strong>2010</strong> Vijittra Leardkamolkarn et al.<br />
No.662<br />
Author (s) : Lee, K., Vivithanaporn, P., Siemieniuk, R.A., Krentz, H.B., Maingat, F., Gill, M.J., Power, C.<br />
Title : Clinical outcomes and immune benefits <strong>of</strong> anti-epileptic drug therapy in HIV/AIDS<br />
Source : BMC Neurology, Volume 10, 17 June <strong>2010</strong>, Article number 44<br />
Document Type : Article<br />
Keywords : anticonvulsive agent, antiretrovirus agent, calcium channel blocking agent, carbamazepine, CD4 antigen, CD8<br />
antigen, etiracetam, gabapentin, lamotrigine, phenobarbital, phenytoin, pregabalin, primidone, sodium channel blocking<br />
agent, topiramate, valproic acid; acquired immune deficiency syndrome, adult, alanine aminotransferase blood level, alkaline<br />
phosphatase blood level, article, aspartate aminotransferase blood level, CD4+ T lymphocyte, CD8+ T<br />
lymphocyte, controlled study, disease duration, drug fatality, drug indication, epilepsy, female, headache, human, human<br />
cell, Human immunodeficiency virus, Human immunodeficiency virus infection, hyperbilirubinemia, immune status, in vitro<br />
study, inhibition kinetics, liver dysfunction, liver function test, lymphocyte count, lymphocyte proliferation, major clinical<br />
study, male, mood disorder, motor dysfunction, neuropathic pain, peripheral neuropathy, prescription, seizure, side effect, T<br />
lymphocyte, treatment duration, treatment outcome, virus load, virus replication, cell culture, cell proliferation, cohort<br />
analysis, drug effect, immunology, metabolism, middle aged, physiology, time, virology; Acquired Immunodeficiency<br />
Syndrome, Adult, Anticonvulsants, Antigens, CD4, Antigens, CD8, Calcium Channel Blockers, Cell Proliferation, Cells,<br />
Cultured, Cohort Studies, Female, HIV, HIV Infections, Humans, Male, Middle Aged, Sodium Channel Blockers, T-<br />
Lymphocytes, Time Factors, Treatment Outcome, Valproic Acid, Virus Replication<br />
<strong>Abstract</strong> : Background: Anti-epileptic drugs (AEDs) are frequently prescribed to persons with HIV/AIDS receiving combination antiretroviral<br />
therapy (cART) although the extent <strong>of</strong> AED use and their interactions with cART are uncertain. Herein, AED usage, associated<br />
toxicities and immune consequences were investigated.Methods: HIV replication was analysed in proliferating human T cells<br />
during AED exposure. Patients receiving AEDs in a geographically-based HIV care program were assessed using clinical and<br />
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laboratory variables in addition to assessing AED indication, type, and cumulative exposures.Results: Valproate suppressed<br />
proliferation in vitro <strong>of</strong> both HIV-infected and uninfected T cells (p
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Document Type : Article<br />
Keywords : Desbutyl-benflumetol; Drug synergism; Lumefantrine; Malaria; Plasmodium falciparum<br />
<strong>Abstract</strong> : The pharmacodynamic interaction between lumefantrine and monodesbutyl-benflumetol has been investigated in 44 fresh<br />
isolates <strong>of</strong> patients with a Plasmodium falciparum infection from the region <strong>of</strong> Mae Sot (Thailand). Both substances proved to<br />
be effective against parasite maturation within the test concentration range, with monodesbutyl-benflumetol being effective<br />
at a lower concentration than lumefantrine. Synergism between the two substances was evaluated with a combination <strong>of</strong><br />
lumefantrine and monodesbutyl-benflumetol at a ratio <strong>of</strong> 4.25:1. The geometric mean values for complete inhibition <strong>of</strong><br />
schizont maturation were 1035.7 nM for lumefantrine, 655 nM for monodesbutyl-benflumetol and 222.5 nM for the<br />
combination <strong>of</strong> both. An analysis for interaction according to the method <strong>of</strong> Berenbaum indicates a moderate synergism at<br />
the IC 50, which gets stronger with increasing ICs and reaches the highest level at the IC99. The geometric mean <strong>of</strong> the sums<br />
<strong>of</strong> the FIC 50 is 0.73, <strong>of</strong> the FIC90 it is 0.37 and <strong>of</strong> the FIC 99 it is 0.25. © <strong>2010</strong> Springer-Verlag.<br />
No.666<br />
Author (s) : Leeboonngam, T.; Pramong, R.; Wongchitrat, P.; Govitrapong, P.; Phansuwan-Pujito, P.<br />
Title : DEVELOPMENT OF MELATONIN RECEPTOR EXPRESSION IN HIPPOCAMPUS OF POSTNATAL RATS<br />
Source : JOURNAL OF NEUROCHEMISTRY, Volume: 115, Supplement: 1, Pages: 15-16, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.667<br />
Author (s) : Leechor, P., Pompanomchai, C., Sukklay, P.<br />
Title : Operation <strong>of</strong> a radio-controlled car by voice commands<br />
Source : ICMEE <strong>2010</strong> - <strong>2010</strong> 2nd <strong>International</strong> Conference on Mechanical and Electronics Engineering, Proceedings, Volume 1, <strong>2010</strong>,<br />
Article number 5558606, Pages V114-V117<br />
Document Type : Conference Paper<br />
Keywords : Human voice commands; Radio-controlled car (RC car); Voice recognition<br />
<strong>Abstract</strong> : The objective <strong>of</strong> this research is to operate a radio-controlled car (RC car) by using Thai voice commands. The research was<br />
conducted by sending a user's voice commands to a computer machine, and then the computer converted the voice<br />
commands into digital signal. After that, the digital signal was converted into a radio wave commands. Finally, an RC car<br />
operated based on the radio wave commands. The experiment was conducted on the RC car with five Thai voice commands,<br />
which were: 1) Stop, 2) Forward, 3) Backward, 4) Turn Left, and 5) Turn Right. The experimental precisions are 98.2%, 93.0%<br />
and 44.2% in a quiet room, an <strong>of</strong>fice room and a noisy room, respectively. © <strong>2010</strong> IEEE.<br />
No.668<br />
Author (s) : Leekitcharoenphon, P., Taweemuang, U., Palittapongarnpim, P., Kotewong, R., Supasiri, T., Sonthayanon, B.<br />
Title : Predicted sub-populations in a marine shrimp proteome as revealed by combined EST and cDNA data from multiple Penaeus<br />
species<br />
Source : BMC Research Notes, Volume 3, <strong>2010</strong>, Article number 295<br />
Document Type : Article<br />
Keywords : Animalia, Artemia, Artemia franciscana, Caenorhabditis elegans, Carcinus maenas, Cladocera, Crustacea, Daphnia, Daphnia<br />
pulex, Decapoda (Crustacea), Drosophila melanogaster, Fenneropenaeus chinensis, Hexapoda, Homarus<br />
americanus, Litopenaeus vannamei, Marsupenaeus japonicus, Penaeidae, Penaeus, Penaeus monodon, Siphonaptera (fleas)<br />
<strong>Abstract</strong> : Background. Many species <strong>of</strong> marine shrimp in the Family Penaeidae, viz. Penaeus (Litopenaeus) vannamei, Penaeus<br />
monodon, Penaeus (Fenneropenaeus) chinensis, and Penaeus (Marsupenaeus) japonicus, are animals <strong>of</strong> economic importance<br />
in the aquaculture industry. Yet information about their DNA and protein sequences is lacking. In order to predict their<br />
collective proteome, we combined over 270,000 available EST and cDNA sequences from the 4 shrimp species with all<br />
protein sequences <strong>of</strong> Drosophila melanogaster and Caenorhabditis elegans. EST data from 4 other crustaceans, the crab<br />
Carcinus maenas, the lobster Homarus americanus (Decapoda), the water flea Daphnia pulex, and the brine shrimp Artemia<br />
franciscana were also used. Findings. Similarity searches from EST collections <strong>of</strong> the 4 shrimp species matched 64% <strong>of</strong> the<br />
protein sequences <strong>of</strong> the fruit fly, but only 45% <strong>of</strong> nematode proteins, indicating that the shrimp proteome content is more<br />
similar to that <strong>of</strong> an insect than a nematode. Combined results with 4 additional non-shrimp crustaceans increased matching<br />
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to 78% <strong>of</strong> fruit fly and 56% <strong>of</strong> nematode proteins, suggesting that present shrimp EST collections still lack sequences for<br />
many conserved crustacean proteins. Analysis <strong>of</strong> matching data revealed the presence <strong>of</strong> 4 EST groups from shrimp, namely<br />
sequences for proteins that are both fruit fly-like and nematode-like, fruit fly-like only, nematode-like only, and nonmatching.<br />
Gene ontology pr<strong>of</strong>iles <strong>of</strong> proteins for the 3 matching EST groups were analyzed. For non-matching ESTs, a small<br />
fraction matched protein sequences from other species in the UniProt database, including other crustacean-specific proteins.<br />
Conclusions. Shrimp ESTs indicated that the shrimp proteome is comprised <strong>of</strong> sub-populations <strong>of</strong> proteins similar to those<br />
common to both insect and nematode models, those present specifically in either model, or neither. Combining small EST<br />
collections from related species to compensate for their small size allowed prediction <strong>of</strong> conserved expressed protein<br />
components encoded by their uncharacterized genomes. The organized data should be useful for transferring annotation<br />
data from model species into shrimp data and for further studies on shrimp proteins with particular functions or groups. ©<br />
<strong>2010</strong> Sonthayanon et al; licensee BioMed Central Ltd.<br />
No.669<br />
Author (s) : Leelahavarong, P., Chaikledkaew, U., Hongeng, S., Kasemsup, V., Lubell, Y., Teerawattananon, Y.<br />
Title : A cost-utility and budget impact analysis <strong>of</strong> allogeneic hematopoietic stem cell transplantation for severe thalassemic<br />
patients in Thailand<br />
Source : BMC Health Services Research, Volume 10, <strong>2010</strong>, Article number 209<br />
Document Type : Article<br />
Keywords : adolescent, adult, article, budget, child, cost benefit analysis, economics, hematopoietic stem cell<br />
transplantation, hospitalization, human, infant, preschool child, probability, quality adjusted life year, Thailand, thalassemia;<br />
Adolescent, Adult, Budgets, Child, Child, Preschool, Cost-Benefit Analysis, Hematopoietic Stem Cell<br />
Transplantation, Humans, Infant, Markov Chains, Quality-Adjusted Life Years, Severity <strong>of</strong> Illness<br />
Index, Thailand, Thalassemia, Young Adult<br />
<strong>Abstract</strong> : Background. Hematopoietic stem cell transplantation (HSCT) is the only curative treatment available to severe thalassemic<br />
patients. The treatment, however, is very costly, particularly in the context <strong>of</strong> low and middle income countries, and no<br />
studies have been carried out to explore its economic justifiability. This study aimed to estimate the cost-utility <strong>of</strong> HSCT<br />
compared with blood transfusions combined with iron chelating therapy (BT-ICT) for severe thalassemia in Thailand, and to<br />
investigate the affordability <strong>of</strong> HSCT using a budget impact analysis. Methods. A Markov model was used to estimate the<br />
relevant costs and health outcomes over the patients' lifetimes taking a societal perspective as recommended by Thailand's<br />
health technology assessment guidelines. All future costs and outcomes were discounted at a rate <strong>of</strong> 3% per annum. Primary<br />
outcomes <strong>of</strong> interest were lifetime costs, quality adjusted life years (QALYs) gained, and the incremental cost-effectiveness<br />
ratio (ICER) in Thai baht (THB) per QALY gained. Results. Compared to BT-ICT, the incremental cost-effectiveness ratio<br />
increased with patient age from 80,700 to 183,000 THB per QALY gained for related HSCT and 209,000 to 953,000 THB per<br />
QALY gained for unrelated HSCT among patients aged 1 to 15 years (US$1= 34 THB). The governmental budget impact<br />
analysis showed that providing 200 related HSCT to patients aged 1 to 10 years, in accordance with the current infrastructure<br />
limitations, would initially require approximately 90 million additional THB per year. Conclusions. At a societal willingness to<br />
pay <strong>of</strong> 100,000 THB per QALY gained, related HSCT was likely to be a cost-effective and affordable treatment for young<br />
children with severe thalassemia in Thailand. © <strong>2010</strong> Leelahavarong et al; licensee BioMed Central Ltd.<br />
No.670<br />
Author (s) : Leelakusolvong, S., Sriprayoon, T., Methasate, A., Akaraviputh, T.<br />
Title : Endoscopic treatment <strong>of</strong> acute Ascaris pancreatitis<br />
Source : Endoscopy, Volume 42, Issue SUPPL. 2, <strong>2010</strong>, Pages E12-E13<br />
Document Type : Article<br />
Keywords : amylase, mebendazole, triacylglycerol lipase; abdominal tenderness, adult, amylase blood level, article, ascariasis, bile duct<br />
dilatation, case report, catheter, computer assisted tomography, disease duration, duodenum, endoscopic retrograde<br />
cholangiopancreatography, endoscopic therapy, epigastric pain, fever, human, leukocyte count, male, nematode, pancreas<br />
duct, pancreatitis, pancreatography, physical examination, priority journal, sphincterotomy, swelling, triacylglycerol lipase<br />
blood level, Vater papilla; Adult, Animals, Antinematodal Agents, Ascariasis, Ascaris lumbricoides, Cholangiopancreatography,<br />
Endoscopic Retrograde, Humans, Male, Mebendazole, Pancreatitis, Sphincterotomy, Endoscopic<br />
<strong>Abstract</strong> : –<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.671<br />
Author (s) : Leelawat, K., Narong, S., Chaijan, S., Sa-Ngiamsuntorn, K., Disthabanchong, S., Wongkajornsilp, A., Hongeng, S.<br />
Title : Proteomic pr<strong>of</strong>iles <strong>of</strong> mesenchymal stem cells induced by a liver differentiation protocol<br />
Source : <strong>International</strong> Journal <strong>of</strong> Molecular Sciences, Volume 11, Issue 12, December <strong>2010</strong>, Pages 4905-4915<br />
Document Type : Article<br />
Keywords : Human mesenchymal stem cells; Liver differentiation; Proteomic study<br />
<strong>Abstract</strong> : The replacement <strong>of</strong> disease hepatocytes and the stimulation <strong>of</strong> endogenous or exogenous regeneration by human<br />
mesenchymal stem cells (MSCs) are promising candidates for liver-directed cell therapy. In this study, we isolated MSCs from<br />
adult bone marrow by plastic adhesion and induced differentiation with a liver differentiation protocol. Western blot analyses<br />
were used to assess the expression <strong>of</strong> liver-specific markers. Next, MSC-specific proteins were analyzed with two-dimensional<br />
(2D) gel electrophoresis and peptide mass fingerprinting matrix-assisted laser desorption/ionization (MALDI)-time <strong>of</strong> flight<br />
(TOF)-mass spectrometry (MS). To confirm the results from the proteomic study, semi-quantitative reverse transcriptionpolymerase<br />
chain reaction (RT-PCR) analyses were performed. We demonstrated that MSCs treated with the liver<br />
differentiation protocol expressed significantly more albumin, CK19 and CK20, than did undifferentiated cells. In addition the<br />
results <strong>of</strong> proteomic study demonstrated increases expression <strong>of</strong> FEM1B, PSMC2 and disulfide-isomerase A3 in MSCs treated<br />
with the liver differentiation protocol. These results from proteomic pr<strong>of</strong>iling will not only provide insight into the global<br />
responses <strong>of</strong> MSCs to hepatocyte differentiation, but will also lead to in-depth studies on the mechanisms <strong>of</strong> proteomic<br />
changes in MSCs. © <strong>2010</strong> by the authors.<br />
No.672<br />
Author (s) : Leerasiri, P., Chayachinda, C., Indhavivadhana, S., Wongwananurak, T., Dangrat, C., Rattanachaiyanont, M.<br />
Title : Nutritional supplements in health-conscious pre-/post-menopausal Thai women<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 10, October <strong>2010</strong>, Pages 1128-1136<br />
Document Type : Article<br />
Keywords : Attitude; Knowledge; Menopause; Nutritional supplement<br />
<strong>Abstract</strong> : Objective: To survey prevalence, knowledge, attitude, and factors affecting the behavior <strong>of</strong> nutritional supplement<br />
consumption in health-conscious pre-/post-menopausal Thai women. Material and Method: A survey was conducted in 327<br />
women, aged 40-67 years, who were new participants in the health promotion education program <strong>of</strong> Siriraj Menopause Clinic,<br />
Gynecologic Endocrinology Unit, Department <strong>of</strong> Obstetrics and Gynecology, Faculty <strong>of</strong> Medicine Siriraj Hospital, <strong>Mahidol</strong><br />
<strong>University</strong>, between January and July 2008. The participants completed a self-administering questionnaire inquiring their<br />
demographic data, attitude, knowledge, and behavior <strong>of</strong> supplement consumption. The participants were divided into three<br />
groups, i.e. current-, past-, and never-users, and then compared. Results: The majority (54.7%) <strong>of</strong> the presented participants<br />
consumed at least one kind <strong>of</strong> supplements, 37.3% and 17.4% were current-and past-users, respectively. The current-, past-,<br />
and never-users were not different in age, medical diseases, education levels, reading frequency, and economic status. The<br />
majority <strong>of</strong> participants obtained the information <strong>of</strong> supplements from newspaper or magazine. The users primarily purchased<br />
the supplements from hospitals or clinics. All participants had knowledge on health promotion and supplement products,<br />
however, 7.6% and 11.5% <strong>of</strong> such knowledge, respectively, was inaccurate. All participants had both positive and negative<br />
attitudes on supplement consumption, the negative attitude was found in 33.4% <strong>of</strong> the items tested. The never-users had<br />
trivial but statistically significant (p < 0.001) more accurate knowledge and more negative attitude than other groups had.<br />
Conclusion: More than 50% <strong>of</strong> health-conscious pre-/post-menopausal Thai women are ever-users <strong>of</strong> nutritional<br />
supplements. A lot <strong>of</strong> misunderstanding and misconception do exist even in the health-conscious women, regardless <strong>of</strong> their<br />
consumption behaviors. More education on this issue should be provided in order to reduce unnecessary expenditure and<br />
increase the efficiency <strong>of</strong> supplement consumption expenditure.<br />
No.673<br />
Author (s) : Leewansangtong, S., Vorrakitkatorn, P., Amornvesukit, T., Taweemonkongsap, T., Nualyong, C., Sujijantararat, P.<br />
Title : Laparo-endoscopic single site (LESS) robotic radical prostatectomy in an Asian man with prostate cancer: An initial case report<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 3, March <strong>2010</strong>, Pages 383-387<br />
Document Type : Article<br />
Keywords : Laparo-endoscopic single site surgery; Prostate neoplasm; Radical prostatectomy; Robotic surgery<br />
<strong>Abstract</strong> : Objective: To report the feasibility <strong>of</strong> laparo-endoscopic single site (LESS) robotic radical prostatectomy performed in Asian<br />
man. Material and Method: A 71 year-old man with adenocarcinoma <strong>of</strong> prostate presented at Faculty <strong>of</strong> Medicine Siriraj<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Hospital, Bangkok. Prostate-specific antigen level was 16.5 ng/ml and digital rectal examination approximately showed 30<br />
gram prostate with nodule in both lobes. No clinical metastasis was found. Leuprorelin acetate and 50 mg <strong>of</strong> bicalutamide<br />
were used for 3 months. The patient's body mass index was 22 and healthy. With the consent form signed, laparo-endoscopic<br />
single site (LESS) robotic radical prostatectomy was performed with the robot daVinci S system. Results: The total operative<br />
time was 335 minutes; docking time was 12 minutes; console time was 275 minutes. The estimate blood loss was 250 ml and<br />
no blood transfusion required. No intraoperative or post-operative complication was found. Jackson drain was removed within<br />
60 hours after surgery. The patient was discharged from the hospital within 84 hours after surgery. The urethral catheter was<br />
removed within 14 days after surgery Conclusion: Laparo-endoscopic single site (LESS) robotic radical prostatectomy is<br />
feasible to be performed. In the initial experience, patient selection is required.<br />
No.674<br />
Author (s) : Leeyaphan, C., Kulthanan, K., Jongjarearnprasert, K., Dhana, N.<br />
Title : Drug-induced angioedema without urticaria: Prevalence and clinical features<br />
Source : Journal <strong>of</strong> the European Academy <strong>of</strong> Dermatology and Venereology, Volume 24, Issue 6, June <strong>2010</strong>, Pages 685-691<br />
Document Type : Article<br />
Keywords : Angioedema; Clinical features; Drug-induced; Prevalence<br />
<strong>Abstract</strong> : Background Angioedema without urticaria can be caused by drugs. The purpose <strong>of</strong> our study was to assess the prevalence<br />
and clinical features <strong>of</strong> patients with drug-induced angioedema without urticaria. Methods This study retrospectively reviewed<br />
case records at Siriraj Hospital, between January 2007 and December 2008. Patients aged at least 15 years were included.<br />
Results The prevalence <strong>of</strong> drug-induced angioedema without urticaria among patients with adverse drug reactions was<br />
2.3%/year. Non-steroidal anti-inflammatory drugs (NSAID) were the most common cause (50%), followed by antibiotics (20%).<br />
The commonest NSAID which induced angioedema were ibupr<strong>of</strong>en and dicl<strong>of</strong>enac. The common sites were periorbital area<br />
(67.3%) and lips (27.6%). The median duration <strong>of</strong> suspected drug therapy before the development <strong>of</strong> angioedema was 1 day<br />
with the range <strong>of</strong> 10 min to 23 days. Conclusions Non-steroidal anti-inflammatory drugs and antibiotics were the most<br />
common drugs causing angioedema without urticaria. The duration <strong>of</strong> onset ranged from minutes to days. After stopping the<br />
suspected drugs, symptoms disappeared within 2-5 days in most patients. © 2009 European Academy <strong>of</strong> Dermatology and<br />
Venereology.<br />
No.675<br />
Author (s) : Leimanis, M.L., Jaidee, A., Sriprawat, K., Kaewpongsri, S., Suwanarusk, R., Barends, M., Phyo, A.P., Russell, B., Renia, L., Nosten, F.<br />
Title : Plasmodium vivax susceptibility to ferroquine<br />
Source : Antimicrobial Agents and Chemotherapy, Volume 54, Issue 5, May <strong>2010</strong>, Pages 2228-2230<br />
Document Type : Article<br />
Keywords : 4 aminoquinoline derivative, antimalarial agent, artesunate, chloroquine, ferroquine, mefloquine, piperaquine, ssr<br />
97193, unclassified drug; antibiotic sensitivity, antimalarial activity, article, drug efficacy, drug metabolism, drug sensitivity, IC<br />
50, malaria falciparum, minimum inhibitory concentration, nonhuman, parasite isolation, Plasmodium vivax malaria, priority<br />
journal, schizont, structure activity relation; Aminoquinolines, Antimalarials, Dose-Response Relationship, Drug, Drug<br />
Resistance, Bacterial, Ferrous Compounds, Humans, Malaria, Vivax, Microbial Sensitivity Tests, Plasmodium vivax, Thailand<br />
<strong>Abstract</strong> : The novel organometallic chloroquine analog ferroquine (SSR 97193) is effective against chloroquine-resistant Plasmodium<br />
falciparum. The ex vivo efficacy <strong>of</strong> ferroquine against Plasmodium vivax isolates was tested. Ferroquine has a potent ex vivo<br />
effect on P. vivax schizont maturation (median 50% inhibitory concentration, 15 nM; n = 42). No significant cross-sensitivity<br />
between ferroquine and other antimalarials was detected. This drug may be a suitable replacement for chloroquine in the<br />
treatment <strong>of</strong> drug-resistant P. vivax malaria. Copyright © <strong>2010</strong>, American Society for Microbiology. All Rights Reserved.<br />
No.676<br />
Author (s) : Lek-Uthai, U., Sangsayan, J., Kachenchart, B., Kulpradit, K., Sujirarat, D., Honda, K.<br />
Title : Novel ellipsoid spatial analysis for determining malaria risk at the village level<br />
Source : Acta Tropica, Volume 116, Issue 1, October <strong>2010</strong>, Pages 51-60<br />
Document Type : Article<br />
Keywords : Ellipsoid distribution; Geographical information system (GIS); Malaria risk; Spatial analysis; Village level<br />
<strong>Abstract</strong> : The distribution patterns <strong>of</strong> malaria incidence at a village level in Thailand were demonstrated with the use <strong>of</strong> a geographical<br />
information system (GIS), and provided the study <strong>of</strong> the malaria situation at a household level. Mosaic imageries from aerial<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
photographs were used to create maps that contained X and Y coordinates. These digitized base maps were kept as<br />
computerized files. Standard Distance Ellipse (SDE) was used to measure the prevalence <strong>of</strong> dispersion around the mean<br />
center <strong>of</strong> malaria cases and points. Households in the SDE were at greater risk <strong>of</strong> malaria infection than those located outside<br />
the SDE. The spatial pattern <strong>of</strong> malaria incidence was investigated using spatial autocorrelation using Geary's ratio and Moran's<br />
index. Five <strong>of</strong> seven villages had a clustered spatial distribution <strong>of</strong> malaria incidence, the vector point <strong>of</strong> which had a 2-3. km<br />
range from the patient's houses. Only one village had a significant clustered spatial distribution <strong>of</strong> malaria incidence (p
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
algorithm and 42-58% when genotype was added (P = 0.002). After several days <strong>of</strong> therapy, a pharmacogenetic algorithm<br />
estimates the therapeutic warfarin dose more accurately than one using clinical factors and INR response alone.<br />
No.680<br />
Author (s) : Leonardi, M.C., Garusi, C., Santoro, L., Dell'Acqua, V., Rossetto, F., Didier, F., Vischioni, B., De Lorenzi, F., Lohsiriwat, V., Yves<br />
Petit, J., Orecchia, R.<br />
Title : Impact <strong>of</strong> medical discipline and observer gender on cosmetic outcome evaluation in breast reconstruction using transverse<br />
rectus abdominis myocutaneous (TRAM) flap and radiotherapy<br />
Source : Journal <strong>of</strong> Plastic, Reconstructive and Aesthetic Surgery, Volume 63, Issue 12, December <strong>2010</strong>, Pages 2091-2097<br />
Document Type : Conference Paper<br />
Keywords : Breast cancer; Cosmesis evaluation; Plastic reconstruction; Radiotherapy<br />
<strong>Abstract</strong> : Background: Despite the complication rate, the majority <strong>of</strong> studies report a satisfactory cosmetic outcome in patients<br />
undergoing transverse rectus abdominis myocutaneous (TRAM) flap breast reconstruction both before and after radiotherapy<br />
(RT). The lack <strong>of</strong> a universal agreement on the use <strong>of</strong> a validated scale for cosmetic assessment in clinical practise leads to<br />
subjective criteria <strong>of</strong> evaluation and causes a great deal <strong>of</strong> interobserver variability. This study investigates whether there is<br />
any difference in the evaluation <strong>of</strong> cosmesis according to gender and specialisation <strong>of</strong> the observer. Methods: Fifty-two<br />
photographs <strong>of</strong> the patients who had undergone TRAM reconstruction for breast cancer, divided into three groups according<br />
to the treatment (TRAM only, TRAM→RT, RT→TRAM), were evaluated by 21 specialists, 10 male and 11 female from<br />
radiotherapy, breast surgery and plastic reconstructive surgery. Cosmetic outcome was classified using the four-category<br />
Harvard scale: a score <strong>of</strong> excellent/good was considered acceptable. Results: The overall rate <strong>of</strong> good/excellent ratings was<br />
66.6%, which was lower than the score reported in the literature. A significantly worse score was registered in the TRAM→RT<br />
group compared with the other groups. The probability for male physicians to award a positive judgement is 24% higher than<br />
that <strong>of</strong> female ones. In general, there is a decent agreement among the judgement raters. Conclusions: No statistically<br />
significant difference in cosmetic evaluation was noted overall between male physicians and female ones. However, within<br />
each specialisation, the difference between the two genders was great. Breast surgeons gave the worst opinion, and among<br />
them female surgeons judged most severely, whereas plastic surgeons gave the best opinion, and among them females<br />
provided the highest favourable judgement. © 2009 British Association <strong>of</strong> Plastic, Reconstructive and Aesthetic Surgeons.<br />
Published by Elsevier Ltd. All rights reserved.<br />
No.681<br />
Author (s) : Leopairut, J., Larbcharoensub, N., Cheewaruangroj, W., Sungkanuparph, S., Sathapatayavongs, B.<br />
Title : Rhin<strong>of</strong>acial entomophthoramycosis; a case series and review <strong>of</strong> the literature<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 4, July <strong>2010</strong>, Pages 928-935<br />
Document Type : Review<br />
Keywords : Conidiobolus spp; Entomophthoramycosis; Phycomycosis; Rhin<strong>of</strong>acial; Zygomycosis<br />
<strong>Abstract</strong> : Rhin<strong>of</strong>acial entomophthoramycosis is an uncommon chronic mycotic disease caused by exposure to the organism<br />
Conidiobolus coronatus. The authors report a case series <strong>of</strong> 5 patients with rhin<strong>of</strong>acial entomophthoramycosis and review the<br />
literature. All patients had typical involvement <strong>of</strong> the rhin<strong>of</strong>acial area with formation <strong>of</strong> subcutaneous lesions causing a<br />
chronic granulomatous inflammatory response with tissue eosinophilia and Splendore-Hoeppli reaction. Diagnoses were made<br />
based on histopathologic examination in all cases and fungi were isolated and identified in one case. The clinicopathologic<br />
features and therapeutic management <strong>of</strong> rhin<strong>of</strong>acial entomophthoramycosis are described.<br />
No.682<br />
Author (s) : Leowattana, W., Tangpukdee, N., Thar, S.K., Nakasiri, S., Srivilairit, S., Kano, S., Wilairatana, P., Krudsood, S.<br />
Title : Changes in platelet count in uncomplicated and severe falciparum Malaria<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 5, September <strong>2010</strong>, Pages 1035-1041<br />
Document Type : Article<br />
Keywords : Platelet counts; Severe malaria; Thrombocytopenia; Uncomplicated malaria<br />
<strong>Abstract</strong> : This study investigated alterations in platelet counts pre- and post-treatment with artemisinin derivatives in uncomplicated<br />
and severe falciparum malaria. Serial platelet counts were taken over 4 weeks for 110 uncomplicated and 110 severe<br />
falciparum malaria patients admitted to the Hospital for Tropical Diseases during 2005-2008. On admission, prior to treatment,<br />
thrombocytopenia was found in 73.6% <strong>of</strong> uncomplicated falciparum malaria patients and 90.9% <strong>of</strong> severe falciparum malaria<br />
249
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
cases. Platelet levels significantly lower in severe malaria cases. Although initial platelet counts were lower than normal in<br />
both study groups, they slowly increased significantly over time, and approached normal levels by several weeks posttreatment.<br />
No bleeding was evident during treatment, and none <strong>of</strong> the patients required a platelet transfusion. Platelet<br />
transfusions are not required for malaria patients with thrombocytopenia who have no bleeding.<br />
No.683<br />
Author (s) : Lerdkiattikorn, P.; Chaikledkaew, U.; Kingkaew, P.; Teerawattananon, Y.<br />
Title : A SYSTEMATIC REVIEW AND META-ANALYSIS OF ADJUVANT CHEMOTHERAPY FOR STAGE III COLON CANCER<br />
Source : VALUE IN HEALTH, Volume: 13, Issue: 7, Pages: A503-A503, Published: NOV <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.684<br />
Author (s) : Lertkiatmongkol, P., Pethuan, S., Jirakanjanakit, N., Rongnoparut, P.<br />
Title : Transcription analysis <strong>of</strong> differentially expressed genes in insecticide-resistant Aedes aegypti mosquitoes after deltamethrin<br />
exposure<br />
Source : Journal <strong>of</strong> Vector Ecology, Volume 35, Issue 1, June <strong>2010</strong>, Pages 197-203<br />
Document Type : Article<br />
Keywords : Aedes aegypti; Deltamethrin; Insecticide resistance; Suppression subtractive hybridization; Tomosyn<br />
<strong>Abstract</strong> : Aedes aegypti mosquitoes are resistant to various insecticides, including pyrethroids, throughout Thailand. We previously<br />
reported that Ae. aegypti from Mae Wong district, Nakhon Sawan Province in north-central Thailand, were resistant to<br />
insecticides, including pyrethroids (deltamethrin and permethrin), organophosphates and carbamates, and that high levels <strong>of</strong><br />
detoxification enzymes were present. In the present study we used the method <strong>of</strong> suppression by subtractive hybridization to<br />
determine differential expression <strong>of</strong> genes in Mae Wong Ae. aegypti that survived the exposure to increasing doses (∼ 1.5 - 2<br />
× 10-5M) <strong>of</strong> deltamethrin beyond the diagnostic dose compared to unexposed mosquitoes. Screening <strong>of</strong> 350 cDNA clones<br />
from the suppression subtractive library by cDNA array hybridization revealed that 58 clones were over-expressed in the<br />
mosquito that survived high dose deltamethrin. The over-expressed cDNA insert sequences corresponded to 11 functional<br />
genes, five hypothetical protein genes, and five sequences <strong>of</strong> unknown function that could be located on the supercontig <strong>of</strong><br />
the Ae. aegypti genome. The functional genes are those coding for cuticular proteins, muscle proteins, proteins related to<br />
controlling the release <strong>of</strong> synaptic vesicles, and other genes such as heat shock protein and small subunit ribosomal RNA.<br />
Over-expression <strong>of</strong> tomosyn and myosin light chain kinase genes was verified using a semiquantitative reverse transcriptionpolymerase<br />
chain reaction (RT-PCR), confirming their increased expression in response to deltamethrin exposure in insecticideresistant<br />
Ae. aegypti.<br />
No.685<br />
Author (s) : Lertmanorat, Z.; Durand, D.M.<br />
Title : Integrated Multiplexer for Nerve Electrodes<br />
Source : Electronic Design, Test and Application, <strong>2010</strong>. DELTA '10. Fifth IEEE <strong>International</strong> Symposium on Issue Date: 13-15 Jan. <strong>2010</strong> On<br />
page(s): 237 - 237<br />
Document Type : Conference Paper<br />
Keywords : Flat interface nerve electrode (FINE), functional electrical stimulation (FES), neural prostheses, selective stimulation.<br />
<strong>Abstract</strong> : Multiple contact nerve cuff electrodes require many leads, making their implantation difficult and potentially damaging to the<br />
nerve. Therefore the design <strong>of</strong> circuits capable <strong>of</strong> reducing the number <strong>of</strong> wires is crucial to the development <strong>of</strong> a device that<br />
can be implanted in patients. The flat interface nerve electrode (FINE) was developed to allow selective recording and<br />
selective stimulation capabilities. We report here the design <strong>of</strong> multiplexers embedded within the cuff electrode that can<br />
reduce the number <strong>of</strong> leads needed to control thirty two channels. The electrode design includes thirty-two contacts in a<br />
1mmx8mm opening. Each contact size is 300μm x400μm with access resistance less than 1kW. The control circuit was<br />
implemented on a polyimide film using <strong>of</strong>fthe- shelf surface mounted electronic components. The electronic module was<br />
mounted directly onto the electrode’s flat substrate. Two control circuit implementations for the control <strong>of</strong> the thirty two<br />
channels were designed, built and tested; 1) a single supply design with only two wires but limited to cathodic-first pulse and<br />
2) a dual-supply design requiring three lead wires but an arbitrary stimulation waveform. These circuit designs allow a<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
significant reduction <strong>of</strong> the number <strong>of</strong> leads required for the control <strong>of</strong> the electrode. However, the hermetic sealing <strong>of</strong> the<br />
devices has not yet been implemented. (Funding was provided the National Institutes <strong>of</strong> Health, grant number<br />
5R01NS032845-14.)<br />
No.686<br />
Author (s) : Lertsukprasert, K., Kasemkosin, N., Cheewareungroj, W., Kasemsuwan, L.<br />
Title : Listening and speaking ability <strong>of</strong> Thai deaf children in preschool aural rehabilitation program<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 4, April <strong>2010</strong>, Pages 474-480<br />
Document Type : Article<br />
Keywords : Hearing-impaired children; Listening and speaking ability; Preschool aural rehabilitation program<br />
<strong>Abstract</strong> : Background: An auditory-oral approach can help deaf children achieve success in oral communication. Many studies confirm<br />
that deaf children with access to sound through high-powered and appropriate hearing aids at the youngest age possible have<br />
the capability to acquire communication skills similar to their hearing peers. Objective: Evaluate the listening and speaking<br />
progress made by 27 Thai hearing-impaired children who attended a preschool aural rehabilitation program, which was<br />
established at Audiology and Speech clinic. After hearing aids fitting, deaf children were enrolled to the preschool aural<br />
rehabilitation program after receiving their parents consent. Material and Method: Hearing impaired children were divided into<br />
groups <strong>of</strong> 4-6 children with approximately the same level <strong>of</strong> performance. The listening and speaking performance at the<br />
initial period were recorded. Each group participated in the 3-hour-program once a week, included auditory training,<br />
conversation (maternal reflexive method), and speech stimulation. The improvements and problems <strong>of</strong> each child were<br />
recorded at the end <strong>of</strong> session. Listening and speaking performance evaluation were recorded at six months intervals. Results:<br />
There were 12 boys and 15 girls. The average hearing loss in the better ear was 104 dBHL, range from 83-117 dBHL, SD = 8.33.<br />
The mean age <strong>of</strong> enrollment was 2 years and 10 months. The majority gradually developed listening skills and speaking<br />
ability. There was no relationship between age <strong>of</strong> enrollment and the listening and speaking ability (p > 0.05). However,<br />
listening skills had positive relationship with length <strong>of</strong> speech (r = 0.685), number <strong>of</strong> spoken vocabulary (r = 0.665), and<br />
speech character (r = 0.598); p < 0.01. Conclusion: Auditory training is an important task to develop listening skills and<br />
improve length <strong>of</strong> speech, speaking vocabulary, and speech character. Other benefits from the aural rehabilitation program<br />
included monitoring the auditory progression after hearing aid fitting, parents meeting, and promotion a better quality <strong>of</strong> life<br />
by enabling hearing impaired children to participate in hearing society.<br />
No.687<br />
Author (s) : Lertwanich, P., Martins, C.A.Q., Kato, Y., Ingham, S.J.M., Kramer, S., Linde-Rosen, M., Smolinski, P., Fu, F.H.<br />
Title : Contribution <strong>of</strong> the menisc<strong>of</strong>emoral ligament as a restraint to the posterior tibial translation in a porcine knee<br />
Source : Knee Surgery, Sports Traumatology, Arthroscopy, Volume 18, Issue 9, <strong>2010</strong>, Pages 1277-1281<br />
Document Type : Article<br />
Keywords : Biomechanics; In situ force; Menisc<strong>of</strong>emoral ligament; Pig; Porcine; Posterior cruciate ligament<br />
<strong>Abstract</strong> : The menisc<strong>of</strong>emoral ligament (MFL) is a major structure in the posterior aspect <strong>of</strong> the porcine knee together with the<br />
posterior cruciate ligament (PCL). While the porcine knee is a frequently used animal model for biomechanical evaluation <strong>of</strong><br />
PCL reconstruction techniques, the contribution <strong>of</strong> the MFL to stability <strong>of</strong> the porcine knee is not well understood. The<br />
purpose <strong>of</strong> this study is (1) to evaluate the kinematics <strong>of</strong> the knee after sequential cutting <strong>of</strong> the PCL and MFL and (2) to<br />
determine the in situ forces <strong>of</strong> the PCL and MFL in response to a posterior tibial load <strong>of</strong> 89 N using the robotic/universal<br />
force-moment sensor system from 15° to 90° <strong>of</strong> knee flexion. Ten porcine knees were used in this study. The magnitude <strong>of</strong><br />
posterior tibial translation under a posterior tibial load was significantly increased (P < 0.01) after sequential transection <strong>of</strong> the<br />
PCL and the MFL at each testing angle compared to the intact condition. The in situ force <strong>of</strong> the PCL was highest at 60° <strong>of</strong><br />
flexion (82.3 ± 8.6 N) and lowest at 15° <strong>of</strong> flexion (45.1 ± 15.9 N). The in situ force <strong>of</strong> the MFL was highest at 15° <strong>of</strong> flexion<br />
(24.3 ± 6.5 N) and lowest at 90° <strong>of</strong> flexion (12.9 ± 10.5 N). The findings in this study revealed a biomechanical contribution <strong>of</strong><br />
the MFL as the secondary restraint to the posterior tibial translation in conjunction with the PCL especially near full<br />
extension. © <strong>2010</strong> Springer-Verlag.<br />
No.688<br />
Author (s) : Letson, G.W., Singhasivanon, P., Fernandez, E., Abeysinghe, N., Amador, J.J., Margolis, H.S., Edelman, R.<br />
Title : Dengue vaccine trial guidelines and role <strong>of</strong> large-scale, post pro<strong>of</strong>-<strong>of</strong>-concept demonstration projects in bringing a dengue<br />
vaccine to use in dengue endemic areas<br />
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Source : Human Vaccines, Volume 6, Issue 10, October <strong>2010</strong>, Pages 802-809<br />
Document Type : Review<br />
Keywords : Clinical trial phases; Demonstration project; Dengue vaccine; Effectiveness; Efficacy; Pro<strong>of</strong> <strong>of</strong> concept<br />
<strong>Abstract</strong> : In this review, we consider the issues impacting conduct and design <strong>of</strong> dengue vaccine trials with reference to the recently<br />
published World Health Organization "Guidelines for Conduct <strong>of</strong> Clinical Trials <strong>of</strong> Dengue Vaccines in Endemic Areas." We<br />
discuss logistic, scientific and ethical challenges concerning evaluation and introduction <strong>of</strong> dengue vaccines; these range from<br />
randomized trials that establish "pro<strong>of</strong> <strong>of</strong> concept" <strong>of</strong> vaccine efficacy, to post-"pro<strong>of</strong> <strong>of</strong> concept" trials, particularly<br />
demonstration projects likely to be required for licensure or for the introduction <strong>of</strong> an already licensed vaccine into public<br />
use. We clarify and define the meaning <strong>of</strong> "pro<strong>of</strong> <strong>of</strong> concept" in the clinical trial context and the meaning <strong>of</strong> terms "phase 2b",<br />
"phase 3b" and "demonstration project", which are commonly used but have not been defined well in the clinical literature.<br />
© <strong>2010</strong> Landes Bioscience.<br />
No.689<br />
Author (s) : Leungwattanakij, S., Watanachote, D., Noppakulsatit, P., Petchpaibuol, T., Choeypunt, N., Tongbai, T., Wanamkang, T.,<br />
Lojanapiwat, B., Permpongkosol, S., Tantiwong, A., Pripatnanont, C., Akarasakul, D., Kongwiwatanakul, S., Chotikawanich, E.<br />
Title : Sexuality and Management <strong>of</strong> Benign Prostatic Hyperplasia with Alfuzosin: SAMBA Thailand<br />
Source : Journal <strong>of</strong> Sexual Medicine, Volume 7, Issue 9, September <strong>2010</strong>, Pages 3115-3126<br />
Document Type : Article<br />
Keywords : Alpha-blocker; Benign prostatic hyperplasia; Ejaculation; Lower urinary tract symptoms; LUTS<br />
<strong>Abstract</strong> : Introduction: Benign prostatic hyperplasia (BPH) is a common condition among elderly men. The aim <strong>of</strong> therapy is to improve<br />
lower urinary tract symptoms (LUTS) and quality <strong>of</strong> life (QoL) and to prevent complications. Aim: The primary objective was<br />
to assess the effect on ejaculatory dysfunction (EjD) <strong>of</strong> 6 months treatment with alfuzosin (XATRAL) 10 mg once daily (OD) in<br />
men with LUTS suggestive <strong>of</strong> BPH in Thailand. Secondary objectives were to evaluate the efficacy <strong>of</strong> alfuzosin on LUTS,<br />
bother score (<strong>International</strong> Prostate Symptom Score [IPSS] 8th question), erectile dysfunction (ED), onset <strong>of</strong> action, and<br />
tolerability. Methods: Overall, 99 men with moderate to severe LUTS suggestive <strong>of</strong> BPH (mean IPSS 18.9, bother score 4.3)<br />
were enrolled in an open-label study. Sexual function was evaluated at baseline and after 6 months treatment, using the<br />
<strong>International</strong> Index <strong>of</strong> Erectile Function-5 and the Male Sexual Health Questionnaire (MSHQ) ejaculation score, a new<br />
validated questionnaire assessing seven EjD symptoms. Main Outcome Measure: The main outcome measure is mean change<br />
from baseline to the end <strong>of</strong> treatment in the MSHQ Ejaculation score. Results: MHSQ ejaculation score significantly improved<br />
from 23.09 at baseline to 21.54 at 6 months (P = 0.022). Overall, 70% <strong>of</strong> patients perceived an improvement in LUTS within 1<br />
week (36.3% within 3 days). IPSS total score significantly improved from 18.93 at baseline to 9.59 at 6 months (P < 0.001). IPSS<br />
voiding and irritative subscores also significantly improved. The percentage <strong>of</strong> patients with moderate or severe ED decreased<br />
from 35.3% at baseline to 21.8% at 6 months. Most adverse events were dizziness (3%) and orthostatic hypotension (1%)<br />
with minor intensity. No significant change in blood pressure and heart rate was observed. Conclusions: Alfuzosin 10 mg OD<br />
administered for 6 months provides a marked and rapid (within 1 week) improvement in LUTS and bother score while<br />
improving both ED and EjD. © <strong>2010</strong> <strong>International</strong> Society for Sexual Medicine.<br />
No.690<br />
Author (s) : Lévesque, C.A., Brouwer, H., Cano, L., Hamilton, J.P., Holt, C., Huitema, E., Raffaele, S., Robideau, G.P., Thines, M., Win, J.,<br />
Zerillo, M.M., Beakes, G.W., Boore, J.L., Busam, D., Dumas, B., Ferriera, S., Fuerstenberg, S.I., Gachon, C.M.M., Gaulin, E., Govers,<br />
F., Grenville-Briggs, L., Horner, N., Hostetler, J., Jiang, R.H.Y., Johnson, J., Krajaejun, T., Lin, H., Meijer, H.J.G., Moore, B., Morris, P.,<br />
Phuntmart, V., Puiu, D., Shetty, J., Stajich, J.E., Tripathy, S., Wawra, S., van West, P., Whitty, B.R., Coutinho, P.M., Henrissat, B.,<br />
Martin, F., Thomas, P.D., Tyler, B.M., De Vries, R.P., Kamoun, S., Yandell, M., Tisserat, N., Buell, C.R.<br />
Title : Genome sequence <strong>of</strong> the necrotrophic plant pathogen Pythium ultimum reveals original pathogenicity mechanisms and<br />
effector repertoire<br />
Source : Genome Biology, Volume 11, Issue 7, 13 July <strong>2010</strong>, Article number R73<br />
Document Type : Article<br />
Keywords : cadherin, cutinase, antifungal agent, protein; abiotic stress, article, carbohydrate metabolism, controlled study, Crinkler<br />
gene, enzyme activity, fungal gene, gene expression, gene function, gene sequence, nonhuman, nucleotide sequence, plant<br />
pathogen interaction, protein degradation, proteomics, Pythium, pythium ultimum, sequence homology, species<br />
comparison, synteny, virulence, DNA sequence, drug effect, gene order, gene<br />
rearrangement, genetics, genome, genomics, growth, development and aging, host pathogen<br />
252
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
interaction, human, microbiology, mitochondrial genome, multigene family, nucleotide<br />
repeat, pathogenicity, phylogeny, plant, secretion, sequence alignment;<br />
Oomycetes, Peronosporaceae, Phytophthora, Phytophthora infestans, Pythium, Pythium ultimum, Solanum tuberosum;<br />
Antifungal Agents, Base Sequence, Cadherins, Carbohydrate Metabolism, Gene Order, Gene<br />
Rearrangement, Genome, Genome, Mitochondrial, Genomics, Host-Pathogen Interactions, Humans, Multigene<br />
Family, Phylogeny, Plants, Proteins, Pythium, Repetitive Sequences, Nucleic Acid, Sequence Alignment, Sequence Analysis,<br />
DNA, Synteny<br />
<strong>Abstract</strong> : Background: Pythium ultimum is a ubiquitous oomycete plant pathogen responsible for a variety <strong>of</strong> diseases on a broad range<br />
<strong>of</strong> crop and ornamental species.Results: The P. ultimum genome (42.8 Mb) encodes 15,290 genes and has extensive<br />
sequence similarity and synteny with related Phytophthora species, including the potato blight pathogen Phytophthora<br />
infestans. Whole transcriptome sequencing revealed expression <strong>of</strong> 86% <strong>of</strong> genes, with detectable differential expression <strong>of</strong><br />
suites <strong>of</strong> genes under abiotic stress and in the presence <strong>of</strong> a host. The predicted proteome includes a large repertoire <strong>of</strong><br />
proteins involved in plant pathogen interactions, although, surprisingly, the P. ultimum genome does not encode any classical<br />
RXLR effectors and relatively few Crinkler genes in comparison to related phytopathogenic oomycetes. A lower number <strong>of</strong><br />
enzymes involved in carbohydrate metabolism were present compared to Phytophthora species, with the notable absence <strong>of</strong><br />
cutinases, suggesting a significant difference in virulence mechanisms between P. ultimum and more host-specific oomycete<br />
species. Although we observed a high degree <strong>of</strong> orthology with Phytophthora genomes, there were novel features <strong>of</strong> the P.<br />
ultimum proteome, including an expansion <strong>of</strong> genes involved in proteolysis and genes unique to Pythium. We identified a<br />
small gene family <strong>of</strong> cadherins, proteins involved in cell adhesion, the first report <strong>of</strong> these in a genome outside the<br />
metazoans.Conclusions: Access to the P. ultimum genome has revealed not only core pathogenic mechanisms within the<br />
oomycetes but also lineage-specific genes associated with the alternative virulence and lifestyles found within the<br />
pythiaceous lineages compared to the Peronosporaceae. © <strong>2010</strong> Lévesque et al.; licensee BioMed Central Ltd.<br />
No.691<br />
Author (s) : Li, A., Russell, B., Renia, L., Lek-Uthai, U., Nosten, F., Lim, C.T.<br />
Title : High density <strong>of</strong> 'spiky' excrescences covering the surface <strong>of</strong> an erythrocyte infected with Plasmodium malariae<br />
Source : British Journal <strong>of</strong> Haematology, Volume 151, Issue 1, October <strong>2010</strong>, Page 1<br />
Document Type : Article<br />
Keywords : article, atomic force microscopy, cell size, cell structure, endothelium cell, erythrocyte, Plasmodium malariae<br />
infection, polymerase chain reaction, priority journal; Erythrocyte Membrane, Erythrocytes, Humans, Malaria, Microscopy,<br />
Atomic Force, Plasmodium malariae<br />
<strong>Abstract</strong> : –<br />
No.692<br />
Author (s) : Li, L., Borland, R., Yong, H., Fong, G.T., Bansal-travers, M., Quah, A.C.K., Sirirassamee, B., Omar, M., Zanna, M.P., Fotuhi, O.<br />
Title : Predictors <strong>of</strong> smoking cessation among adult smokers in Malaysia and Thailand: Findings from the <strong>International</strong> Tobacco<br />
Control Southeast Asia survey<br />
Source : Nicotine and Tobacco Research, Volume 12, Issue SUPPL. 1, October <strong>2010</strong>, Pages S34-S44<br />
Document Type : Article<br />
Keywords : abstinence, adult, article, demography, female, health survey, human, major clinical study, Malaysia, male, multivariate<br />
analysis, predictor variable, priority journal, self concept, smoking cessation, Thailand, tobacco; Adult, Aged, Attitude to<br />
Health, Female, Follow-Up Studies, Humans, Malaysia, Male, Middle Aged, Motivation, Multivariate<br />
Analysis, Prevalence, Recurrence, Self Efficacy, Smoking, Smoking Cessation, Socioeconomic Factors, Thailand, Tobacco Use<br />
Disorder, Young Adult<br />
<strong>Abstract</strong> : Introduction: Limited longitudinal studies on smoking cessation have been reported in Asia, and it remains unclear whether<br />
determinants <strong>of</strong> quitting are similar to those found in Western countries. This study examined prospective predictors <strong>of</strong><br />
smoking cessation among adult smokers in Thailand and Malaysia. Methods: Four thousand and four smokers were surveyed<br />
in Malaysia and Thailand in 2005. Of these, 2,426 smokers were followed up in 2006 (61% retention). Baseline measures <strong>of</strong><br />
sociodemographics, dependence, and interest in quitting were used to predict both making quit attempts and point<br />
prevalence maintenance <strong>of</strong> cessation. Results: More Thai than Malaysian smokers reported having made quit attempts<br />
between waves, but among those who tried, the rates <strong>of</strong> staying quit were not considerably different between Malaysians<br />
and Thais. Multivariate analyses showed that smoking fewer cigarettes per day, higher levels <strong>of</strong> self-efficacy, and more<br />
253
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
immediate quitting intentions were predictive <strong>of</strong> both making a quit attempt and staying quit in both countries. Previous<br />
shorter quit attempts and higher health concerns about smoking were only predictive <strong>of</strong> making an attempt, whereas prior<br />
abstinence for 6 months or more and older age were associated with maintenance. Discussion: In Malaysia and Thailand,<br />
predictors <strong>of</strong> quitting activity appear to be similar. However, as in the West, predictors <strong>of</strong> making quit attempts are not all the<br />
same as those who predict maintenance. The actual predictors differ in potentially important ways from those found in the<br />
West. We need to determine the relative contributions <strong>of</strong> cultural factors and the shorter history <strong>of</strong> efforts to encourage<br />
quitting in Asia. © The Author <strong>2010</strong>. Published by Oxford <strong>University</strong> Press on behalf <strong>of</strong> the Society for Research on Nicotine<br />
and Tobacco. All rights reserved.<br />
No.693<br />
Author (s) : Li, X., Breukers, C., Ymeti, A., Pattanapanyasat, K., Sukapirom, K., Terstappen, L.W.M.M., Greve, J.<br />
Title : Clinical evaluation <strong>of</strong> a simple image cytometer for CD4 enumeration on hiv-infected patients<br />
Source : Cytometry Part B - Clinical Cytometry, Volume 78, Issue 1, <strong>2010</strong>, Pages 31-36<br />
Document Type : Article<br />
Keywords : AIDS; CD4; CD8; Image cytometry<br />
<strong>Abstract</strong> : Background: Affordable, easy-to-use, and reliable CD4+ T lymphocyte enumeration systems are needed in resourceconstrained<br />
settings to monitor HIV. Methods: A simple image cytometer was used to count fluorescently labeled CD4+ T and<br />
CD8+ T lymphocytes from CD3 immunomagnetically selected cells on blood specimens <strong>of</strong> 460 HIV-1-infected patients in<br />
Siriraj Hospital, Bangkok, Thailand. Results were compared with flow cytometry (FCM). Results: CD4+ T lymphocyte counts by<br />
image cytometer were comparable (R ≥ 0.97) with those by the FACSCount and the FACScan with a bias <strong>of</strong> 7.3 and 9.1%,<br />
respectively. At very low CD4+ T lymphocyte counts (≤50/μl) some over-count outliers were observed by the FACScan and<br />
image cytometer when compared with the FACSCount. For CD8 enumeration, the image cytometer showed a good<br />
correlation (R 5 0.96) and a consistent undercount (-17%) when compared with the FACSCount. Conclusions: Evaluation <strong>of</strong><br />
the image cytometer for CD4 and CD8 enumeration demonstrated comparable results with FCM on a population <strong>of</strong> HIV-1infected<br />
patients. The image cytometer is a good alternative method for point-<strong>of</strong>-care settings in resource-constrained<br />
countries. © 2009 Clinical Cytometry Society.<br />
No.694<br />
Author (s) : Liao, J.-C., Fan, K.-F., Keorochana, G., Chen, W.-J., Chen, L.-H.<br />
Title : Transpedicular grafting after short-segment pedicle instrumentation for thoracolumbar burst fracture: Calcium sulfate cement<br />
versus autogenous iliac bone graft<br />
Source : Spine, Volume 35, Issue 15, 1 July <strong>2010</strong>, Pages 1482-1488<br />
Document Type : Article<br />
Keywords : Autogenous iliac bone graft; Calcium sulfate cement; Thoracolumbar burst fracture<br />
<strong>Abstract</strong> : Study Design. A retrospective clinical and radiographic study was performed. Objective. To compare 2 grafting materials <strong>of</strong><br />
anterior augmentation for thoracolumbar burst fractures: transpedicular cancellous bone (TPCB) grafting and transpedicular<br />
calcium sulfate grafting and to decide whether calcium sulfate cement can replace autogenous cancellous bone applied in<br />
anterior vertebral body augmentation after posterior short-segment instrumentation. Summary <strong>of</strong> Background Data. Additional<br />
TPCB grafting was developed as an alternative to prevent early implant failure. However, the results are inconsistent and<br />
donor-site complications are a major concern. Calcium sulfate has been <strong>of</strong>fered as a bone substitute for treating patients with<br />
metaphysis fractures or bone defect, but the results <strong>of</strong> application in spinal surgeries are uncertain. Methods. Fifty-one<br />
patients with a single-level thoracolumbar burst fracture for treatment with short-segment pedicle screw fixation were<br />
enrolled in the study. Fractures in group 1 patients were reinforced with TPCB (n = 31), and fractures in group 2 patients were<br />
augmented with transpedicular calcium sulfate cement (TPCSC; n = 20). All patients were followed-up at least 2 years after<br />
surgery. Radiographic parameters and clinical outcomes were compared between the 2 groups. Results. The 2 groups were<br />
similar in age, sex, fracture levels, preoperative neurologic status distribution, and the associated injuries. The TPCB group had<br />
a longer period <strong>of</strong> follow-up (52.7 ± 4.9 vs. 28.6 ± 3.5 months, P < 0.001). Blood loss and operation time were less in the<br />
TPCSC group (247.5 ± 164.2 vs. 600.0 ± 403.1 mL, P = 0.001 and 161.7 ± 28.5 vs. 227.2 ± 43.6 minutes, P < 0.001).<br />
Radiographic parameters and clinical outcomes were similar in both groups. The TPCSC group had no surgical complication,<br />
but the TPCB group revealed 2 cases with wound infection after surgery. The failure rate, defined as an increase <strong>of</strong> 10° or<br />
more in loss <strong>of</strong> correction or implant failure, was also not significantly different (TPCB = 9.6% and TPCSC = 15%; P = 0.565).<br />
All patients with partial neurologic deficits initially improved at the final follow-up. Conclusion. Additional TPCB grafting after<br />
254
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
posterior short-segment instrumentation remains a reliable surgical method for correcting and maintaining sagittal alignment<br />
and vertebral body height in thoracolumbar burst fractures. Calcium sulfate cement also proved to be an effective bone<br />
substitute used in spinal surgeries for patients with thoracolumbar burst fractures. © <strong>2010</strong>, Lippincott Williams & Wilkins.<br />
No.695<br />
Author (s) : Liaw, Y. F.; Raptopoulou-Gigi, M.; Cheinquer, H.; Sarin, S. K.; Tanwandee, T.; Leung, N.; Myers, R. P.; Brown, R. S., Jr.; Bialkowska,<br />
J.; Munafo, L.; Chen, Y. C.; Tang, S.; Cooney, E.; Dharane, P.; Newman, J.<br />
Title : Efficacy and safety <strong>of</strong> entecavir (ETV) vs. adefovir (ADV) in chronic hepatitis B (CHB) patients with evidence <strong>of</strong> hepatic<br />
decompensation: ETV-048 study<br />
Source : JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY, Volume: 25, Supplement: 3, Pages: A125-A125, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.696<br />
Author (s) : Liaw, Y. -F.; Raptopoulou-Gigi, M.; Cheinquer, H.; Sarin, S. K.; Tanwandee, T.; Leung, N.; Peng, C. -Y.; Myers, R. P.; Brown, R. S.,<br />
Jr.; Tsai, N.; Bialkowska, J.; Tang, S.; Cooney, E.<br />
Title : RISK AND PREDICTORS OF MORTALITY OR HEPATOCELLULAR CARCINOMA AMONG ENTECAVIR- OR ADEFOVIR-TREATED CHRONIC<br />
HEPATITIS B PATIENTS WITH EVIDENCE OF HEPATIC DECOMPENSATION<br />
Source : JOURNAL OF HEPATOLOGY, Volume: 52, Supplement: 1, Pages: S390-S391, Published: <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.697<br />
Author (s) : Liewluck, T.<br />
Title : Immune-mediated rippling muscle disease: Another inflammatory myopathy in myasthenia gravis<br />
Source : Archives <strong>of</strong> Neurology, Volume 67, Issue 7, July <strong>2010</strong>, Pages 896-897<br />
Document Type : Letter<br />
Keywords : caveolin 3, CD4 antigen, CD8 antigen; autoimmune disease, CD4+ T lymphocyte, CD8+ T lymphocyte, disease<br />
severity, human, immune mediated rippling muscle<br />
disease, immunohistochemistry, immunoreactivity, inflammation, letter, muscle biopsy, muscle necrosis, muscle<br />
stiffness, myalgia, myasthenia gravis, myositis, priority journal, symptom; Caveolin 3, Humans, Muscle, Skeletal, Myasthenia<br />
Gravis, Myositis<br />
<strong>Abstract</strong> : –<br />
No.698<br />
Author (s) : Liewrian, W., Hoonsawat, R., Tang, I.-M.<br />
Title : Spin switching effects in a ferromagnetic graphene junction having a second gate<br />
Source : Physica E: Low-Dimensional Systems and Nanostructures, Volume 42, Issue 5, March <strong>2010</strong>, Pages 1287-1292<br />
Document Type : Article<br />
Keywords : Dirac equation; Ferromagnetic barrier; Graphene; Magnetic tunnel junction<br />
<strong>Abstract</strong> : The effects <strong>of</strong> the bias voltage placed on a second gate which is positioned to the left <strong>of</strong> the ferromagnetic graphene (FG)<br />
layer on the switching <strong>of</strong> the charge and spin transports in a ferromagnetic graphene junction are studied. We show that the<br />
change in the bias voltage from positive to negative (while maintaining the positive voltage on the first gate above the FG<br />
layer) can induce a switching from high to low conductance states in the junction. The voltage on the first gate is used to<br />
shift the Fermi level in the FG layer. Enhancement <strong>of</strong> the on/<strong>of</strong>f conductance ratio on the switching is shown to be inversely<br />
proportional to the thickness <strong>of</strong> the second gate barrier. We show that by biasing the nanostructures with the voltage U2, the<br />
spin polarization can be increased and that as a consequence, the thickness <strong>of</strong> the FG barrier layer needed for spin-polarized<br />
transport can be reduced. © 2009 Elsevier B.V. All rights reserved.<br />
255
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.699<br />
Author (s) : Likidlilid, A., Akrawinthawong, K., Poldee, S., Sriratanasathavorn, C.<br />
Title : Paraoxonase 1 polymorphisms as the risk factor <strong>of</strong> coronary heart disease in a Thai population<br />
Source : Acta Cardiologica, Volume 65, Issue 6, <strong>2010</strong>, Pages 681-691<br />
Document Type : Article<br />
Keywords : Atherosclerosis; Coronary heart disease; Paraoxonase 1; Polymorphism<br />
<strong>Abstract</strong> : Objective - Two common polymorphisms <strong>of</strong> the paraoxonase (PON1) gene, L55M and Q192R, were proven to mitigate<br />
atherosclerosis pathogenesis by protecting lipoproteins against peroxidation. This study was to evaluate the associations<br />
between both PON1 gene polymorphisms in Thai hyper-lipidaemia with and without coronary heart disease (CHD). Methods -<br />
Both PON1 genotypes were determined using PCR-RFLP in 103 healthy control subjects, 103 primary hyperlipidaemia without<br />
history <strong>of</strong> such diseases and 106 angiographically documented CHD patients. Results - The frequencies <strong>of</strong> PON1 192R allele<br />
and 192RR genotype were significantly higher in CHD patients than in normal control subjects (P = 0.009 and 0.037,<br />
respectively). The significantly higher frequencies <strong>of</strong> 55M allele and 55LM genotype were also observed in CHD patients (P =<br />
0.037 and P = 0.034, respectively). The frequencies <strong>of</strong> both PON1 polymorphisms were not different in primary<br />
hyperlipidaemia as compared to the normal control subjects. The odds ratio (OR) <strong>of</strong> 192RR genotype and 192R allele for CHD<br />
were 2.84 (1.17-6.99, P = 0.011) and 1.70 (1.11-2.61, P = 0.009), respectively. The age-adjusted OR for CHD was 2.72 (1.25-5.94,<br />
P = 0.012). These frequencies <strong>of</strong> both PON1 alleles were similar to those seen in other Asian populations. Conclusions - The<br />
association between PON1 polymorphisms and CHD risk was demonstrated in a Thai population.These new data underscore<br />
the essence <strong>of</strong> ethnic variations in the interpretation <strong>of</strong> CHD associated with PON1 polymorphism.<br />
No.700<br />
Author (s) : Likidlilid, A., Patchanans, N., Peerapatdit, T., Sriratanasathavorn, C.<br />
Title : Lipid peroxidation and antioxidant enzyme activities in erythrocytes <strong>of</strong> type 2 diabetic patients<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 6, June <strong>2010</strong>, Pages 682-693<br />
Document Type : Article<br />
Keywords : Antioxidant; Coronary heart disease; Lipid peroxidation; Oxidative stress; Type 2 diabetes<br />
<strong>Abstract</strong> : Background and Objective: Although diabetes mellitus (DM) patients are claimed to be under oxidative stress because <strong>of</strong><br />
prolonged exposure to hyperglycemia, the influence <strong>of</strong> glycemic control and cardiovascular complication in diabetes on<br />
oxidative stress parameters has not been fully studied. The present study aimed to investigate lipid peroxidation end product<br />
(malondialdehyde, MDA) and antioxidant enzymes in fairly controlled type 2 DM (fasting plasma glucose [FPG] ≤ 180 mg/ dl)<br />
or type 2 DM complicated with coronary heart disease (CHD) and poorly controlled type 2 DM (FPG > 180 mg/dl) in<br />
comparison to a normal healthy group (FPG < 110 mg/dl). Material and Method: MDA and antioxidant enzymes such as<br />
superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) were determined in the red cell <strong>of</strong> 19 subjects<br />
with poorly controlled type 2 DM, 26 subjects with fairly controlled type 2 DM and 20 subjects with type 2 DM complicated<br />
with CHD who were matched for age and gender. Twenty healthy subjects with normal plasma glucose level and matched<br />
for age and gender were served as a control group. In all groups <strong>of</strong> DM these oxidative stress parameters were compared to a<br />
control group by one-way ANOVA test. Pearson rank correlation coefficient was used to compare the relationship between<br />
FPG and oxidative stress status in type 2 DM and normal controls. Results: The red cell MDA levels were significantly higher in<br />
all types <strong>of</strong> diabetes compared to age-matched normal controls. The mean <strong>of</strong> red cell MDA level was highest in type 2 DM<br />
complicated with CHD. Red cell antioxidant enzyme activities were also significantly increased except for SOD and GPx<br />
activities in fairly controlled type 2 DM. The significant positive correlation between oxidative stress status (as MDA and CAT)<br />
and FPG was found in poorly controlled type 2 DM and type 2 DM complicated with CHD whereas in fairly controlled type 2<br />
DM the significant positive correlation between CAT and FPG was only observed. Conclusion: These findings strongly<br />
confirmed the evidence that diabetic patients were susceptible to oxidative stress and higher blood glucose level had an<br />
association with free radical-mediated lipid peroxidation. The highest level <strong>of</strong> MDA in type 2 DM complicated with CHD<br />
suggested that oxidative stress played an important role in the pathogenesis <strong>of</strong> cardiovascular complication. The results also<br />
showed the increase in antioxidant enzymes. These could probably be due to adaptive response to pro-oxidant in diabetic<br />
state. Hence, there seems to be imbalance between oxidant and antioxidant systems in type 2 diabetic patients.<br />
No.701<br />
Author (s) : Likidlilid, A.; Peerapatdit, T.; Hobang, N.; Tresukosol, D.; Ratanamaneechat, S.<br />
256
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : THE A148G POLYMORPHISM IN THE PARAOXONASE-2 GENE INCREASES THE RISK OF TYPE 2 DIABETIC MELLITUS AND<br />
CORONARY HEART DISEASE<br />
Source : ATHEROSCLEROSIS SUPPLEMENTS, Volume: 11, Issue: 2, Pages: 199-199, Meeting <strong>Abstract</strong>: MS442, Published: JUN 10 <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.702<br />
Author (s) : Likitsuntonwong, W.; Ampawong, S.<br />
Title : The Effects <strong>of</strong> Using Tramadol Hydrochloride as Preoperative Analgesia on the Anesthetic Impact <strong>of</strong> Tiletamine/Zolazepam in<br />
Sprague-Dawley Rat<br />
Source : JOURNAL OF THE AMERICAN ASSOCIATION FOR LABORATORY ANIMAL SCIENCE, Volume: 49, Issue: 5, Pages: 748-748,<br />
Published: SEP <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.703<br />
Author (s) : Limmathurotsakul, D., Jamsen, K., Arayawichanont, A., Simpson, J.A., White, L.J., Lee, S.J., Wuthiekanun, V., Chantratita, N.,<br />
Cheng, A., Day, N.P.J., Verzilli, C., Peacock, S.J.<br />
Title : Defining the true sensitivity <strong>of</strong> culture for the diagnosis <strong>of</strong> melioidosis using Bayesian latent class models<br />
Source : PLoS ONE, 5 (8), art. no. e12485, .<br />
Document Type : Article<br />
Keywords : antiinfective agent; adult, aged, article, bacterium culture, Bayes theorem, controlled study, culture technique, diabetes<br />
mellitus, diagnostic accuracy, diagnostic value, echography, female, human, major clinical study, male, melioidosis, sensitivity<br />
and specificity, serology, treatment response, factual database, methodology, microbiology, middle<br />
aged, reproducibility, standard; Bacteria (microorganisms), Negibacteria, Nucleopolyhedrovirus; Adult, Aged, Bayes<br />
Theorem, Culture Techniques, Databases, Factual, Female, Humans, Male, Melioidosis, Middle Aged, Reference<br />
Standards, Reproducibility <strong>of</strong> Results, Sensitivity and Specificity<br />
<strong>Abstract</strong> : Background: Culture remains the diagnostic gold standard for many bacterial infections, and the method against which other<br />
tests are <strong>of</strong>ten evaluated. Specificity <strong>of</strong> culture is 100% if the pathogenic organism is not found in healthy subjects, but the<br />
sensitivity <strong>of</strong> culture is more difficult to determine and may be low. Here, we apply Bayesian latent class models (LCMs) to<br />
data from patients with a single Gram-negative bacterial infection and define the true sensitivity <strong>of</strong> culture together with the<br />
impact <strong>of</strong> misclassification by culture on the reported accuracy <strong>of</strong> alternative diagnostic tests. Methods/Principal Findings:<br />
Data from published studies describing the application <strong>of</strong> five diagnostic tests (culture and four serological tests) to a patient<br />
cohort with suspected melioidosis were re-analysed using several Bayesian LCMs. Sensitivities, specificities, and positive and<br />
negative predictive values (PPVs and NPVs) were calculated. Of 320 patients with suspected melioidosis, 119 (37%) had<br />
culture confirmed melioidosis. Using the final model (Bayesian LCM with conditional dependence between serological tests),<br />
the sensitivity <strong>of</strong> culture was estimated to be 60.2%. Prediction accuracy <strong>of</strong> the final model was assessed using a classification<br />
tool to grade patients according to the likelihood <strong>of</strong> melioidosis, which indicated that an estimated disease prevalence <strong>of</strong><br />
61.6% was credible. Estimates <strong>of</strong> sensitivities, specificities, PPVs and NPVs <strong>of</strong> four serological tests were significantly different<br />
from previously published values in which culture was used as the gold standard. Conclusions/Significance: Culture has low<br />
sensitivity and low NPV for the diagnosis <strong>of</strong> melioidosis and is an imperfect gold standard against which to evaluate<br />
alternative tests. Models should be used to support the evaluation <strong>of</strong> diagnostic tests with an imperfect gold standard. It is<br />
likely that the poor sensitivity/specificity <strong>of</strong> culture is not specific for melioidosis, but rather a generic problem for many<br />
bacterial and fungal infections. © <strong>2010</strong> Limmathurotsakul et al.<br />
No.704<br />
Author (s) : Limmathurotsakul, D., Wongratanacheewin, S., Teerawattanasook, N., Wongsuvan, G., Chaisuksant, S., Chetchotisakd, P.,<br />
Chaowagul, W., Day, N.P.J., Peacock, S.J.<br />
Title : Increasing incidence <strong>of</strong> human melioidosis in northeast Thailand<br />
Source : American Journal <strong>of</strong> Tropical Medicine and Hygiene, 82 (6), pp. 1113-1117.<br />
257
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Document Type : Article<br />
Keywords : adult, article, bacterium culture, Burkholderia infection, cause <strong>of</strong> death, cohort analysis, diabetes<br />
mellitus, female, human, incidence, major clinical study, male, melioidosis, prospective study, risk factor, sex<br />
difference, Thailand; Age Distribution, Diabetes Mellitus, Female, Humans, Incidence, Male, Melioidosis, Middle Aged, Risk<br />
Factors, Thailand; Bacteria (microorganisms), Burkholderia pseudomallei, Human immunodeficiency virus, Negibacteria<br />
<strong>Abstract</strong> : Melioidosis is a serious community-acquired infectious disease caused by the Gram-negative environmental bacterium<br />
Burkholderia pseudomallei. A prospective cohort study identified 2,243 patients admitted to Sappasithiprasong Hospital in<br />
northeast Thailand with culture-confirmed melioidosis between 1997 and 2006. These data were used to calculate an<br />
average incidence rate for the province <strong>of</strong> 12.7 cases <strong>of</strong> melioidosis per 100,000 people per year. Incidence increased<br />
incrementally from 8.0 (95% confidence interval [CI] = 7.2-10.0) in 2000 to 21.3 (95% CI = 19.2-23.6) in 2006 (P < 0.001; χ2<br />
test for trend). Male sex, age ≥ 45 years, and either known or undiagnosed diabetes were independent risk factors for<br />
melioidosis. The average mortality rate from melioidosis over the study period was 42.6%. The minimum estimated<br />
population mortality rate from melioidosis in 2006 was 8.63 per 100,000 people (95% CI = 7.33-10.11), the third most<br />
common cause <strong>of</strong> death from infectious diseases in northeast Thailand after human immunodeficiency virus (HIV)/acquired<br />
immunodeficiency syndrome (AIDS) and tuberculosis. Copyright © <strong>2010</strong> by The American Society <strong>of</strong> Tropical Medicine and<br />
Hygiene.<br />
No.705<br />
Author (s) : Limmathurotsakul, D., Wuthiekanun, V., Chantratita, N., Wongsuvan, G., Amornchai, P., Day, N.P.J., Peacock, S.J.<br />
Title : Burkholderia pseudomallei is spatially distributed in soil in Northeast Thailand<br />
Source : PLoS Neglected Tropical Diseases, 4 (6), art. no. e694, .<br />
Document Type : Article<br />
Keywords : accuracy, agricultural land, article, bacterial count, bacterium culture, Burkholderia pseudomallei, controlled<br />
study, geographic distribution, mathematical model, nonhuman, quantitative analysis, sampling, soil analysis, soil<br />
micr<strong>of</strong>lora, species distribution, surface soil, Thailand, Burkholderia, demography, geography, isolation and<br />
purification, microbiology, normal distribution; Burkholderia, Colony Count, Microbial, Demography, Geography, Normal<br />
Distribution, Soil Microbiology, Thailand<br />
<strong>Abstract</strong> : Background: Melioidosis is a frequently fatal infectious disease caused by the soil dwelling Gram-negative bacterium<br />
Burkholderia pseudomallei. Environmental sampling is important to identify geographical distribution <strong>of</strong> the organism and<br />
related risk <strong>of</strong> infection to humans and livestock. The aim <strong>of</strong> this study was to evaluate spatial distribution <strong>of</strong> B. pseudomallei<br />
in soil and consider the implications <strong>of</strong> this for soil sampling strategies. Methods and Findings: A fixed-interval sampling<br />
strategy was used as the basis for detection and quantitation by culture <strong>of</strong> B. pseudomallei in soil in two environmental sites<br />
(disused land covered with low-lying scrub and rice field) in northeast Thailand. Semivariogram and indicator semivariogram<br />
were used to evaluate the distribution <strong>of</strong> B. pseudomallei and its relationship with range between sampling points. B.<br />
pseudomallei was present on culture <strong>of</strong> 80/100 sampling points taken from the disused land and 28/100 sampling points<br />
from the rice field. The median B. pseudomallei cfu/gram from positive sampling points was 378 and 700 for the disused land<br />
and the rice field, respectively (p = 0.17). Spatial autocorrelation <strong>of</strong> B. pseudomallei was present, in that samples taken from<br />
areas adjacent to sampling points that were culture positive (negative) for B. pseudomallei were also likely to be culture<br />
positive (negative), and samples taken from areas adjacent to sampling points with a high (low) B. pseudomallei count were<br />
also likely to yield a high (low) count. Ranges <strong>of</strong> spatial autocorrelation in quantitative B. pseudomallei count were 11.4<br />
meters in the disused land and 7.6 meters in the rice field. Conclusions: We discuss the implications <strong>of</strong> the uneven<br />
distribution <strong>of</strong> B. pseudomallei in soil for future environmental studies, and describe a range <strong>of</strong> established geostatistical<br />
sampling approaches that would be suitable for the study <strong>of</strong> B. pseudomallei that take account <strong>of</strong> our findings. © <strong>2010</strong><br />
Limmathurotsakul et al.<br />
No.706<br />
Author (s) : Limpanuparb, T., Punyain, K., Tantirungrotechai, Y.<br />
Title : A DFT investigation <strong>of</strong> methanolysis and hydrolysis <strong>of</strong> triacetin<br />
Source : Journal <strong>of</strong> Molecular Structure: THEOCHEM, 955 (1-3), pp. 23-32.<br />
Document Type : Article<br />
Keywords : Biodiesel; Density Functional Theory; Hydrolysis; Methanolysis; Triacetin<br />
258
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : The thermodynamic and kinetic aspects <strong>of</strong> the methanolysis and hydrolysis reactions <strong>of</strong> glycerol triacetate or triacetin, a<br />
model triacylglycerol compound, were investigated by using Density Functional Theory (DFT) at the B3LYP/6-31++G(d,p) level<br />
<strong>of</strong> calculation. Twelve elementary steps <strong>of</strong> triacetin methanolysis were studied under acid-catalyzed and base-catalyzed<br />
conditions. The mechanism <strong>of</strong> acid-catalyzed methanolysis reaction which has not been reported yet for any esters was<br />
proposed. The effects <strong>of</strong> substitution, methanolysis/hydrolysis position, solvent and face <strong>of</strong> nucleophilic attack on the free<br />
energy <strong>of</strong> reaction and activation energy were examined. The prediction confirmed the facile position at the middle position<br />
<strong>of</strong> glycerol observed by NMR techniques. The calculated activation energy and the trends <strong>of</strong> those factors agree with existing<br />
experimental observations in biodiesel production. © <strong>2010</strong> Elsevier B.V.<br />
No.707<br />
Author (s) : Limroongreungrat, W., Jamkrajang, P., Tongaim, R.<br />
Title : Upper extremity kinematics during free throw shooting <strong>of</strong> thai wheelchair basketball players<br />
Source : IFMBE Proceedings, 31 IFMBE, pp. 213-215.<br />
Document Type : Conference Paper<br />
Keywords : Free-throw shooting; Kinematics; Wheelchair Basketball<br />
<strong>Abstract</strong> : Wheelchair basketball (WCB) is one <strong>of</strong> the fastest growing competitive team sports. To score points, free-throw shooting (FTS)<br />
is the easiest shot since it is an uncontested shot. Although previous studies have been investigated kinematic <strong>of</strong> FTS,<br />
kinematics <strong>of</strong> upper extremity has never been reported in Thai WCB players. Understanding upper extremity kinematics is<br />
valuable since it can help improve shooting performance. Thus, the purpose <strong>of</strong> this study was to study upper extremity<br />
kinematic during free throws <strong>of</strong> advanced WCB players. Five male WCB athletes (meanage = 27 + 2.9 yrs, meanweight 61 +<br />
4.2 Kg) volunteered in the study. Participants were divided into two groups: high class (4-4.5 points) and low class (3 - 3.5<br />
points). Seven retroreflective markers were placed on the right side <strong>of</strong> second metacarpophalangeal joint, the fifth<br />
metacarpophalangeal joint, and the radial and ulna styloid processes, the medial and lateral epicondyles and acromion<br />
process. Three video cameras were simultaneously recorded FTS motion at the sampling frequency <strong>of</strong> 30 Hz and<br />
synchronized by the light switch. Raw coordinate data were filtered with 4 th order Butterworth with a cut-<strong>of</strong>f frequency <strong>of</strong> 4<br />
Hz. Each participant performed FTS for 10 trials which two successful trials <strong>of</strong> clean shot were averaged and analyzed. Upper<br />
extremity angles including shoulder, elbow and wrist were obtained. The Mann-Whitney U test was employed to determine<br />
statistical significance between the groups. The results showed that the high class had a greater range <strong>of</strong> motion <strong>of</strong> shoulder<br />
and elbow than the low class group whereas the low class group used a larger range <strong>of</strong> motion than the high group. Since<br />
the low class group used smaller range <strong>of</strong> motion at the shoulder and elbow joint, they may compensate at the wrist joint.<br />
Nevertheless, no statistical differences <strong>of</strong> upper extremity angles between the two groups were found (p<0.05). Due to the<br />
small sample size, future investigation with larger sample size is warranted. © <strong>2010</strong> <strong>International</strong> Federation for Medical and<br />
Biological Engineering.<br />
No.708<br />
Author (s) : Limroongreungrat, W.; Sutthajinda, P.; Tongaim, R.; Wang, Y.T.<br />
Title : Muscle Activation Patterns <strong>of</strong> during a Straight Punch <strong>of</strong> Young Thai National Amateur Boxers<br />
Source : MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, Volume: 42, Issue: 5, Supplement: 1, Pages: 678-678, Meeting <strong>Abstract</strong>:<br />
2585, Published: MAY <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.709<br />
Author (s) : Limsuwan, A.<br />
Title : Correlation between the 6-min walk test and exercise stress test<br />
Source : Acta Paediatrica, <strong>International</strong> Journal <strong>of</strong> Paediatrics, 99 (7), pp. 958-959.<br />
Document Type : Letter<br />
Keywords : body weight, child, exercise test, human, letter, oxygen consumption, priority journal, school child, screening test, six minute<br />
walk test<br />
<strong>Abstract</strong> : –<br />
259
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.710<br />
Author (s) : Limsuwan, A., Pienvichit, P., Limpijankit, T., Khowsathit, P., Hongeng, S., Pornkul, R., Siripornpitak, S., Boonbaichaiyapruk, S.<br />
Title : Transcoronary bone marrow-derived progenitor cells in a child with myocardial infarction: First pediatric experience<br />
Source : Clinical Cardiology, 33 (8), pp. E7-E12.<br />
Document Type : Article<br />
Keywords : CD133 antigen, CD34 antibody, granulocyte colony stimulating factor; article, bone marrow cell, case report, child, clinical<br />
feature, congestive heart failure, echocardiography, female, heart infarction, heart left ventricle ejection<br />
fraction, human, nuclear magnetic resonance imaging, outcome assessment, school child, stem cell mobilization, stem cell<br />
transplantation; Antigens, CD, Antigens, CD34, Bone Marrow Cells, Bone Marrow Transplantation, Cell<br />
Separation, Child, Echocardiography, Female, Flow Cytometry, Glycoproteins, Granulocyte Colony-Stimulating Factor, Heart<br />
Catheterization, Heart Failure, Humans, Magnetic Resonance Imaging, Myocardial Infarction, Peptides, Recovery <strong>of</strong><br />
Function, Stem Cell Transplantation, Stroke Volume, Time Factors, Treatment Outcome, Ventricular Function, Left<br />
<strong>Abstract</strong> : Background: Recent advances in stem cell therapy to restore cardiac function have great promise for patients with congestive<br />
heart failure aftermyocardial infarction in an adult population. Objective: We examined the benefits <strong>of</strong> bone marrow-derived<br />
progenitor cells treatment modality for the pediatric patient. Methods and Results: We present our first case <strong>of</strong> transcoronary<br />
autologous stem cell transplantation in a 9-year-old girl with refractory congestive heart failure secondary to myocardial<br />
infarction 1 year after transcatheter revascularization. The child received daily injections <strong>of</strong> granulocyte colony-stimulating<br />
factor for 3 days prior to the bone marrow aspiration. The bone marrow cells were isolated to constitute CD133+/CD34+<br />
more than 90% <strong>of</strong> the total number. Subsequently, the progenitor cell suspension was injected via a transcoronary catheter<br />
without any complication. Three months after stem cell therapy, her cardiac function, assessed by both cardiac magnetic<br />
resonance and echocardiogram, has been improvedwith the left ventricular ejection fraction at 47% compared to the<br />
baseline <strong>of</strong> 30%. Conclusion: This is the first reported pediatric case <strong>of</strong> successful transcoronary injection <strong>of</strong> bone marrowderived<br />
progenitor cells for end-stage heart disease. The procedure is considered safe and feasible for the pediatric<br />
population. © <strong>2010</strong> Wiley Periodicals, Inc.<br />
No.711<br />
Author (s) : Limsuwan, T., Castells, M.C.<br />
Title : Outcomes and safety <strong>of</strong> rapid desensitization for chemotherapy hypersensitivity<br />
Source : Expert Opinion on Drug Safety, 9 (1), pp. 39-53.<br />
Document Type : Review<br />
Keywords : Adverse drug reactions; Anaphylaxis; Carboplatin; Chemotherapy agents; Hypersensitivity reactions; Paclitaxel; Rapid<br />
desensitization<br />
<strong>Abstract</strong> : Importance <strong>of</strong> the field: The incidence <strong>of</strong> hypersensitivity reactions (HSRs) to chemotherapy agents has increased because <strong>of</strong><br />
increasing number <strong>of</strong> cancer survivors are exposed to repeated courses <strong>of</strong> sensitizing agents. Replacement with an alternative<br />
chemotherapy regimen is <strong>of</strong>ten limited by tumor sensitivity. Rapid desensitization <strong>of</strong>fers an effective mean to allow<br />
continuation <strong>of</strong> the treatment to which patients have presented HSRs. Areas covered in this review: We review the methods,<br />
outcome and safety <strong>of</strong> the rapid desensitization protocol developed at Brigham and Women's Hospital, Harvard Medical<br />
School Affiliate, based on our recent publication "'Hypersensitivity reactions to chemotherapy: outcome and safety <strong>of</strong> rapid<br />
desensitization in 413 cases". Literature search was conducted through Medline (from January 1976 to September 2009), using<br />
PubMed. What the reader will gain: The article will give insight to clinical manifestations <strong>of</strong> immediate HSR to various<br />
chemotherapy agents and their presumably different immunopathomechanism. Risk assessment, including skin testing in<br />
those presented HSRs to platins and details on rapid desensitization process and its pitfalls will be discussed. Take home<br />
message: Standa"rd protocol <strong>of</strong> rapid desensitization, administering under multidisciplinary team approach, is safe and<br />
effective in overcoming immediate HSRs to platins, taxanes, doxorubicin and rituximab via both intravenous and<br />
intraperitoneal routes. © <strong>2010</strong> Informa UK Ltd ISSN.<br />
No.712<br />
Author (s) : Limsuwan, T., Demoly, P.<br />
Title : Acute Symptoms <strong>of</strong> Drug Hypersensitivity (Urticaria, Angioedema, Anaphylaxis, Anaphylactic Shock)<br />
Source : Medical Clinics <strong>of</strong> North America, 94 (4), pp. 691-710.<br />
Document Type : Review<br />
Keywords : Anaphylactic shock; Anaphylaxis; Angioedema; Drug hypersensitivity; Urticaria<br />
260
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : Drug hypersensitivity reactions (HSRs) are the adverse effects <strong>of</strong> drugs which, when taken at doses generally tolerated by<br />
normal subjects, clinically resemble allergy. Immediate-reaction <strong>of</strong> drug HSRs are those that occur less than 1 hour after the<br />
last drug intake, usually in the form <strong>of</strong> urticaria, angioedema, rhinitis, conjunctivitis, bronchospasm, and anaphylaxis or<br />
anaphylactic shock. Acute urticarial and angioedema reactions are common clinical problems frequently encountered by<br />
internists and general practitioners. They are not specific to drug allergic reaction, and can be caused by various pathogenic<br />
mechanisms. Despite the benign course <strong>of</strong> urticaria and angioedema, a mucocutaneous swelling <strong>of</strong> the upper respiratory tract<br />
could be life-threatening by itself or a feature <strong>of</strong> anaphylaxis. This article reviews acute symptoms <strong>of</strong> drug HSR-related<br />
urticaria, angioedema, anaphylaxis, and anaphylactic shock, and how clinicians should approach these problems. © <strong>2010</strong><br />
Elsevier Inc. All rights reserved.<br />
No.713<br />
Author (s) : Limsuwan, T., Thakkinstian, A., Verasertniyom, O., Vanichapuntu, M., Attia, J., Janwityanujit, S., Nantiruj, K.<br />
Title : Possible protective effects <strong>of</strong> the Glu27 allele <strong>of</strong> ß2- Adrenergic receptor polymorphism in Thai asthmatic patients<br />
Source : Asian Pacific Journal <strong>of</strong> Allergy and Immunology, 28 (2-3), pp. 107-114.<br />
Document Type : Article<br />
Keywords : Adrenergic receptor-ß2; Asthma; Genetic polymorphism; Haplotype; Lung function<br />
<strong>Abstract</strong> : The genetic polymorphisms at the 16th (Arg→ Gly) and 27th (Gln→Glu) amino acid positions <strong>of</strong> the ß2-adrenergic receptor<br />
(ADRB2) may be linked to various asthma-related phenotypes. These include the adverse effects on lung function known to<br />
occur following the regular use <strong>of</strong> albuterol. The study aimed to determine the association between these two ADRB2 SNPs,<br />
their haplotypes and the phenotypes in Thai asthmatic patients. One-hundred and thirty asthmatic patients were genotyped<br />
at the Arg16Gly and Gln27Glu polymorphisms. Demographic data, disease severities, pulmonary function tests and medication<br />
usages were recorded for each patient. The frequencies <strong>of</strong> the Arg16 and Gln27 alleles were found to be 56.9% and 91.2%,<br />
respectively, while the linkage disequilibrium coefficient between the two SNPs was 0.36. Three haplotypes were estimated,<br />
i.e. Arg-Gln, Gly-Gln and Gly-Glu with frequencies <strong>of</strong> 148 (56.9%), 89 (34.2%) and 23 (8.9%), respectively. The mean<br />
percentages for predicted FEV1 (%FEV1) for these corresponding haplotypes were 73.5 (SD = 16.3), 72.4 (SD = 17.4) and 80.7<br />
(SD = 13.1), respectively (p = 0.258). Additionally, the number <strong>of</strong> hospitalizations, emergency visits and inhaled<br />
corticosteroid/longacting ß2-agonist (ICS/ LABA) usages were lower in Gln/Glu subjects than for Gln/Gln genotyped patients,<br />
with values <strong>of</strong> 0% versus 11.9% (p = 0.122) for hospitalizations;4.5% versus 18.8% (p = 0.121) for emergency visits; and 50%<br />
versus 76.6%, (p = 0.042) for ICS/LABA usages. The presence <strong>of</strong> the Glu27 allele in Thai asthmatic patients is associated with a<br />
decreased asthma severity, higher %FEV1 values, less frequent hospitalizations and emergency visits, and decreased ICS/LABA<br />
usage.<br />
No.714<br />
Author (s) : Limsuwan, A.; Wongwandee, R.; Khowsathit, P.<br />
Title : Correlation between 6-min walk test and exercise stress test in healthy children<br />
Source : ACTA PAEDIATRICA, Volume: 99, Issue: 3, Pages: 438-441, DOI: 10.1111/j.1651-2227.2009.01602.x, Published: MAR <strong>2010</strong><br />
Document Type : Article<br />
Keywords : Exercise stress test; Functional capacity; Healthy children; Six-minute walk test<br />
<strong>Abstract</strong> : Aim: To investigate the correlation between 6-min walk test (SMWT) and incremental treadmill exercise stress test (EST) as<br />
indicators for the functional capacity in children. Methods: Healthy children aged 9-12 years were included. The<br />
anthropometric data, SMWT and EST were prospectively measured using the standard protocols. Various parameters were<br />
analysed to define the correlation between SMWT and EST. Results: A total <strong>of</strong> 100 subjects (53 boys) aged 10.3 +/- 1.0 years<br />
participated in the study. The SMWT distance was 586.1 +/- 44.0 m. Height (r = 0.59, R(2) = 35%), length <strong>of</strong> the leg (r = 0.64,<br />
R(2) = 41%), heart rate at the end <strong>of</strong> SMWT (r = 0.59, R(2) = 35%) and heart rate difference at the end <strong>of</strong> SMWT (r = 0.71, R(2)<br />
= 50%) were found to have significant correlation with SMWT distance. The estimated maximal oxygen consumption (eVO2)<br />
obtained during the EST tended to be greater in boys than in girls. Among the parameters obtained during EST, maximal heart<br />
rate (r = 0.33, R(2) = 11%) and the eVO2 (r = 0.54, R(2) = 53%) were found to have significant correlation with SMWT.<br />
Conclusions: SMWT distance is significantly correlated with the eVO2 obtained during the EST. This indicates that SMWT is also<br />
one <strong>of</strong> the predictive markers for EST performance.<br />
No.715<br />
Author (s) : Lirdprapamongkol, K., Sakurai, H., Suzuki, S., Koizumi, K., Prangsaengtong, O., Viriyaroj, A., Ruchirawat, S., Svasti, J., Saiki, I.<br />
261
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Vanillin enhances TRAIL-induced apoptosis in cancer cells through inhibition <strong>of</strong> NF-κB activation<br />
Source : In Vivo, Volume 24, Issue 4, July <strong>2010</strong>, Pages 501-506<br />
Document Type : Article<br />
Keywords : Apoptosis; Cervical cancer; NF-κB; TRAIL; Vanillin<br />
<strong>Abstract</strong> : Background: Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a promising anticancer agent which selectively<br />
kills cancer cells with little effect on normal cells. However, TRAIL resistance is widely found in cancer cells. We have<br />
previously reported antimetatstatic and antiangiogenic effects <strong>of</strong> vanillin, a flavoring agent from vanilla. Here we have<br />
evaluated the sensitizing effect <strong>of</strong> vanillin on a TRAIL-resistant human cervical cancer cell line, HeLa. Materials and Methods:<br />
Cell viability after treatments was determined by the WST-1 cell counting kit. Apoptosis was demonstrated by detection <strong>of</strong><br />
caspase-3 activation and cleavage <strong>of</strong> poly (ADP-ribose) polymerase using immunoblot analysis. Effect <strong>of</strong> treatments on TRAIL<br />
signaling pathway and nuclear factor κB (FN-κB) activation was studied using immunoblot analysis and luciferase reporter<br />
assay. Results: Pretreatment <strong>of</strong> HeLa cells with vanillin enhanced TRAIL-induced cell death through the apoptosis pathway.<br />
Vanillin pretreatment inhibited TRAIL-induced phosphorylation <strong>of</strong> p65 and transcriptional activity <strong>of</strong> NF-κB. Conclusion:<br />
Vanillin sensitizes HeLa cells to TRAIL-induced apoptosis by inhibiting NF-κB activation.<br />
No.716<br />
Author (s) : Lithanatudom, P., Leecharoenkiat, A., Wannatung, T., Svasti, S., Fucharoen, S., Smith, D.R.<br />
Title : A mechanism <strong>of</strong> ineffective erythropoiesis in β-thalassemia/Hb E disease<br />
Source : Haematologica, Volume 95, Issue 5, May <strong>2010</strong>, Pages 716-723<br />
Document Type : Article<br />
Keywords : Apoptosis; Calcium; Cd34+ erythroid progenitor cells; Erythroblasts; Stress; Thalassemia; Unfolded protein response; XBP-<br />
1<br />
<strong>Abstract</strong> : Background Cells respond to stress stimuli through a number <strong>of</strong> response pathways, <strong>of</strong> which one <strong>of</strong> the most important and<br />
well characterized is the unfolded protein response. Despite a large body <strong>of</strong> work which suggests that stress in erythroblasts<br />
may play a pivotal role in the pathogenesis <strong>of</strong> β-thalassemia/Hb E disease, this pathway remains uninvestigated. Design and<br />
Methods Day 10 erythroblasts from normal controls and β-thalassemia/Hb E patients were subjected to internal (treatment<br />
with tunicamycin) and external (serum and growth factor withdrawal) stress stimuli and the activation <strong>of</strong> the unfolded protein<br />
response pathway was investigated. Results Normal erythroblasts responded to both internal and external stress by activating<br />
the unfolded protein response (UPR) pathway while in contrast, erythroblasts from β-thalassemia/Hb E patients only showed<br />
activation <strong>of</strong> the unfolded protein response pathway in response to internal stress. This was reflected by a markedly<br />
increased induction <strong>of</strong> apoptosis in serum and growth factor deprived β-thalassemia/Hb E erythroblasts as compared to<br />
control cells. Modulation <strong>of</strong> the levels <strong>of</strong> intracellular Ca2+ in thalassemic erythroblasts restored UPR activation during serum<br />
deprivation and significantly reduced the level <strong>of</strong> serum deprivation induced apoptosis to control levels. Conclusions These<br />
results suggest the failure <strong>of</strong> thalassemic erythroblasts to cope with cellular stress caused by an impaired UPR function as a<br />
result <strong>of</strong> high Ca2+ levels may exacerbate thalassemic cell death during erythropoiesis. © <strong>2010</strong> Ferrata Storti Foundation.<br />
No.717<br />
Author (s) : Liu, L., Mondal, M.M.H., Idris, M.A., Lokman, H.S., Rajapakse, P.R.V.J., Satrija, F., Diaz, J.L., Upatham, E.S., Attwood, S.W.<br />
Title : The phylogeography <strong>of</strong> Indoplanorbis exustus (Gastropoda: Planorbidae) in Asia<br />
Source : Parasites and Vectors, Volume 3, Issue 1, <strong>2010</strong>, Article number 57<br />
Document Type : Article<br />
Keywords : article, Asia, evolution, gastropod, indoplanorbis exustus, nonhuman, nucleotide sequence, phylogenetic<br />
tree, phylogeography; Basommatophora, Bos, Gastropoda, Indoplanorbis, Indoplanorbis exustus, Planorbidae, Schistosoma<br />
<strong>Abstract</strong> : Background: The freshwater snail Indoplanorbis exustus is found across India, Southeast Asia, central Asia (Afghanistan), Arabia<br />
and Africa. Indoplanorbis is <strong>of</strong> economic importance in that it is responsible for the transmission <strong>of</strong> several species <strong>of</strong> the<br />
genus Schistosoma which infect cattle and cause reduced livestock productivity. The snail is also <strong>of</strong> medical importance as a<br />
source <strong>of</strong> cercarial dermatitis among rural workers, particularly in India. In spite <strong>of</strong> its long history and wide geographical range,<br />
it is thought that Indoplanorbis includes only a single species. The aims <strong>of</strong> the present study were to date the radiation <strong>of</strong><br />
Indoplanorbis across Asia so that the factors involved in its dispersal in the region could be tested, to reveal potential<br />
historical biogeographical events shaping the phylogeny <strong>of</strong> the snail, and to look for signs that I. exustus might be<br />
polyphyletic. Results: The results indicated a radiation beginning in the late Miocene with a divergence <strong>of</strong> an ancestral<br />
bulinine lineage into Assam and peninsular India clades. A Southeast Asian clade diverged from the peninsular India clade<br />
262
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
late-Pliocene; this clade then radiated at a much more rapid pace to colonize all <strong>of</strong> the sampled range <strong>of</strong> Indoplanorbis in<br />
the mid-Pleistocene. Conclusions: The phylogenetic depth <strong>of</strong> divergences between the Indian clades and Southeast Asian<br />
clades, together with habitat and parasitological differences suggest that I. exustus may comprise more than one species. The<br />
timescale estimated for the radiation suggests that the dispersal to Arabia and to Southeast Asia was facilitated by<br />
palaeogeographical events and climate change, and did not require human involvement. Further samples from Afghanistan,<br />
Africa and western India are required to refine the phylogeographical hypothesis and to include the African Recent dispersal.<br />
© <strong>2010</strong> Liu et al; licensee BioMed Central Ltd.<br />
No.718<br />
Author (s) : Lohsiriwat, S., Rukkiat, M., Chaikomin, R., Leelakusolvong, S.<br />
Title : Effect <strong>of</strong> ginger on lower esophageal sphincter pressure<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 3, March <strong>2010</strong>, Pages 366-372<br />
Document Type : Article<br />
Keywords : Dyspepsia; Esophageal manometry; Ginger; Lower esophageal sphincter; Wet swallowing; Zingiber <strong>of</strong>ficinale<br />
<strong>Abstract</strong> : Objective: Ginger has been traditionally used to reduce intestinal gas and flatulence. The present study examined the effects<br />
<strong>of</strong> ginger on the esophagus and lower esophageal sphincter (LES) function by esophageal manometry. Study design: A<br />
randomized controlled trial. Setting: Departments <strong>of</strong> Physiology and Medicine, Faculty <strong>of</strong> Medicine Siriraj Hospital. Subjects:<br />
Fourteen healthy young male volunteers. Material and Method: The effect <strong>of</strong> ginger (1 gram <strong>of</strong> dried powder suspended in<br />
100 ml water) on LES and esophageal peristalsis were studied by manometry in 14 healthy young men. Subjects drank 100<br />
ml <strong>of</strong> water as a control, then performed five wet swallows at 30 minutes after the drink, followed by drinking a ginger<br />
suspension and performed five wet swallows at every 30 minutes thereafter for 180 minutes. The esophageal manometry was<br />
performed throughout 180 minutes after ginger consumption. The manometric parameters before and after water and ginger<br />
intake were compared. Results: The present study showed that after 1 gram-ginger consumption, the LES resting pressures<br />
remained unchanged but the percent relaxation at swallowing was increased throughout the 180 minutes with statistical<br />
significance at 90, 150 and 180 minutes. The amplitude and duration <strong>of</strong> esophageal contraction were not changed, while the<br />
velocity <strong>of</strong> contraction waves was decreased at 30, 120, 150 and 180 minutes after the drinks. Conclusion: Ginger did not<br />
affect LES pressure at rest or esophageal contractile amplitude and duration when swallowing, but caused more relaxation <strong>of</strong><br />
the LES and decreased the esophageal contraction velocity, which may cause more chance <strong>of</strong> gastric gas expel or<br />
antiflatulant effect.<br />
No.719<br />
Author (s) : Lohsiriwat, V.<br />
Title : Colonoscopic perforation: Incidence, risk factors, management and outcome<br />
Source : World Journal <strong>of</strong> Gastroenterology, Volume 16, Issue 4, 28 January <strong>2010</strong>, Pages 425-430<br />
Document Type : Review<br />
Keywords : Colonoscopic perforation; Colonoscopy; Complication; Incidence; Management; Outcome; Postpolypectomy<br />
syndrome; Risk factors; Sigmoidoscopy; Treatment<br />
<strong>Abstract</strong> : This review discusses the incidence, risk factors, management and outcome <strong>of</strong> colonoscopic perforation (CP). The incidence<br />
<strong>of</strong> CP ranges from 0.016% to 0.2% following diagnostic colonoscopies and could be up to 5% following some colonoscopic<br />
interventions. The perforations are frequently related to therapeutic colonoscopies and are associated with patients <strong>of</strong><br />
advanced age or with multiple comorbidities. Management <strong>of</strong> CP is mainly based on patients' clinical grounds and their<br />
underlying colorectal diseases. Current therapeutic approaches include conservative management (bowel rest plus the<br />
administration <strong>of</strong> broadspectrum antibiotics), endoscopic management, and operative management (open or laparoscopic<br />
approach). The applications <strong>of</strong> each treatment are discussed. Overall outcomes <strong>of</strong> patients with CP are also addressed. ©<br />
<strong>2010</strong> Baishideng. All rights reserved.<br />
No.720<br />
Author (s) : Lohsiriwat, V., Pongsanguansuk, W., Lertakyamanee, N., Lohsiriwat, D.<br />
Title : Impact <strong>of</strong> metabolic syndrome on the short-term outcomes <strong>of</strong> colorectal cancer surgery<br />
Source : Diseases <strong>of</strong> the Colon and Rectum, Volume 53, Issue 2, February <strong>2010</strong>, Pages 186-191<br />
Document Type : Article<br />
Keywords : Colon cancer; Complication; Metabolic syndrome; Outcome; Rectal cancer<br />
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<strong>Abstract</strong> : Purpose: The purpose <strong>of</strong> this study was to determine the effects <strong>of</strong> metabolic syndrome on the short-term outcomes <strong>of</strong><br />
colorectal cancer surgery. Methods: A prospective, observational study <strong>of</strong> colorectal cancer patients who underwent elective<br />
oncological resection was conducted between June 2007 and August 2008 at the Faculty <strong>of</strong> Medicine, Siriraj Hospital,<br />
Bangkok. Each patient received preoperative screening for metabolic syndrome using the criteria <strong>of</strong> the American Heart<br />
Association and the National Heart, Lung, and Blood Institute. Factors influencing 30-day postoperative morbidity and<br />
mortality were analyzed. Results: The study included 114 patients with colorectal cancer, with an average age <strong>of</strong> 61 years<br />
(range 29-91). Forty-two (36.8%) patients had metabolic syndrome. There was no significant difference in age, sex, tumor, or<br />
operative parameters between patients with or without metabolic syndrome. There was no postoperative mortality, and<br />
overall postoperative morbidity was 21.9%. Patients with metabolic syndrome had a higher rate <strong>of</strong> complications and a longer<br />
length <strong>of</strong> hospital stay than those without metabolic syndrome (40.5% vs 11.1%, P < .001 and 11.2 vs 8.1 day, P = .006,<br />
respectively). The presence <strong>of</strong> metabolic syndrome, tumor location, American Society <strong>of</strong> Anesthesiologists' score III, elevated<br />
blood pressure, and high triglycerides level were risk factors for postoperative complications in the univariate analysis;<br />
however, in the multivariate analysis, the presence <strong>of</strong> metabolic syndrome and rectal cancer surgery were the only 2<br />
independent factors for the development <strong>of</strong> complications. Conclusions: Patients with metabolic syndrome had a higher rate<br />
<strong>of</strong> postoperative complication and a longer length <strong>of</strong> hospital stay than patients without metabolic syndrome. © The ASCRS<br />
<strong>2010</strong>.<br />
No.721<br />
Author (s) : Lohsiriwat, V., Yodying, H., Lohsiriwat, D.<br />
Title : Incidence and factors influencing the development <strong>of</strong> fistula-in-ano after incision and drainage <strong>of</strong> perianal abscesses<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 1, January <strong>2010</strong>, Pages 61-65<br />
Document Type : Article<br />
Keywords : Anal fistula; Anal sepsis; Antibiotics; Incidence; Perianal abscess; Risk factors<br />
<strong>Abstract</strong> : Objective: The aims <strong>of</strong> the present study were to evaluate the incidence <strong>of</strong> fistula-in-ano following incision and drainage <strong>of</strong><br />
acute perianal abscess and to determine factors influencing the fistula formation. Material and Method: Patients with a firsttime<br />
perianal abscess or intersphincteric abscess who underwent incision and drainage at Siriraj Hospital from January 2005 to<br />
June 2008 were reviewed. Patients with coexisting fistula or perianal Crohn's disease were excluded. Incidence and risk factors<br />
for fistula formation were analyzed. Results: Sixty-four patients were reviewed (50 males, 14 females). The average age <strong>of</strong> the<br />
patients was 44 years (range 19-82). The average follow-up period was 30 months (range 10-53). Twenty patients (31%)<br />
developed fistula-in-ano following incision and drainage. Gender, smoking, alcohol consumption, fever, leukocytosis, and<br />
location <strong>of</strong> abscess were not predictive <strong>of</strong> fistula formation. Univariate analysis showed that patients aged under 40 years and<br />
non-diabetic patients tended to have a higher risk for developing the fistula (43% vs. 21%, OR 2.95, 95% CI 0.98-8.85; p = 0.05<br />
and 38% vs. 13%, OR 4.2, 95% CI 0.85-20.83; p = 0.071, respectively). However, patients receiving perioperative antibiotics<br />
(ATB) were less likely to develop subsequent fistula in both univariate and multivariate analysis. The FIA rate in non-ATB<br />
group was 48% and only 17% in ATB group (OR 4.5, 95% CI 1.44-14.13; p = 0.01). Conclusion: The incidence <strong>of</strong> fistula-in-ano<br />
following incision and drainage <strong>of</strong> perianal abscess was 31%. Patients aged under 40 years and non-diabetic patients appeared<br />
to have a higher risk for fistula formation. Administration <strong>of</strong> perioperative antibiotics significantly reduced the rate <strong>of</strong><br />
subsequent fistula formation.<br />
No.722<br />
Author (s) : Lolekha, R., Chunwimaleung, S., Hansudewechakul, R., Leawsrisook, P., Prasitsuebsai, W., Srisamang, P., Wongsawat, J.,<br />
Faikratok, W., Pattanasin, S., Agins, B.D., Fox, K.K., McConnell, M.S.<br />
Title : Pediatric HIVQUAL-T: Measuring and improving the quality <strong>of</strong> pediatric HIV care in Thailand, 2005-2007<br />
Source : Joint Commission Journal on Quality and Patient Safety, Volume 36, Issue 12, December <strong>2010</strong>, Pages 541-551<br />
Document Type : Article<br />
Keywords : antiretrovirus agent; adolescent, article, child, clinical trial, health care quality, hospital management, human, Human<br />
immunodeficiency virus infection, infant, information system, multicenter study, newborn, organization and<br />
management, preschool child, Thailand; Adolescent, Anti-Retroviral Agents, Child, Child, Preschool, HIV Infections, Hospital<br />
Administration, Humans, Infant, Infant, Newborn, Information Systems, Process Assessment (Health Care), Quality Indicators,<br />
Health Care, Quality <strong>of</strong> Health Care, Thailand<br />
<strong>Abstract</strong> : Background: As increasing numbers <strong>of</strong> children initiate antiretroviral treatment (ART), a systematic process is needed to<br />
measure and improve pediatric HIV care quality. Methods: Pediatric HIVQUAL-T, a model for performance measurement and<br />
264
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quality Improvement (QI), was adapted from the U.S. HIVQUAL model by incorporating Thai national guidelines as standards.<br />
In each <strong>of</strong> five pilotsite hospitals in Thailand in 2005-2007, clinical data abstracted from parient records were used to identify<br />
priority areas for QI. Improvement strategies were designed by clinic teams in different care system areas, and indicators were<br />
remeasured in 2006 and 2007. Results: At the five hospitals, 1,119 HIV-infected children younger than 15 years <strong>of</strong> age received<br />
care in 2005, 1,183 in 2006, and 1,341 in 2007-<strong>of</strong> whom 460, 435, and 418, respectively, were selected for chart abstraction.<br />
Of the eligible children, ≥ 95% received clinical monitoring, annual CD4 count monitoring, ART, and adherence and growth<br />
assessments; 60%-90% received Pneumocystis jiroveci pneumonia (PCP) prophylaxis, tuberculosis (TB) screening, oral health<br />
assessments, and HIV disclosure. Indicators with a score ≤ 40% in 2005 but with significant improvement (p
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
quality inside sampled swine buildings. Based on the results given in this paper, recommendations on appropriate feeding<br />
menu, buildings' cleaning schedule and emission control program have been made.<br />
No.725<br />
Author (s) : Lourens, C., Watkins, W.M., Barnes, K.I., Sibley, C.H., Guerin, P.J., White, N.J., Lindegardh, N.<br />
Title : Implementation <strong>of</strong> a reference standard and pr<strong>of</strong>iciency testing programme by the World Wide Antimalarial Resistance<br />
Network (WWARN)<br />
Source : Malaria Journal, Volume 9, Issue 1, <strong>2010</strong>, Article number 375<br />
Document Type : Article<br />
Keywords : antimalarial agent, drug metabolite; accuracy, article, drug efficacy, drug safety, in vitro study, laboratory, malaria, quality<br />
control, standard, drug effect, drug sensitivity, health care quality, human, international cooperation, isolation and<br />
purification, methodology, parasitology, Plasmodium; Antimalarials, Humans, <strong>International</strong> Cooperation, Malaria, Parasitic<br />
Sensitivity Tests, Plasmodium, Quality Assurance, Health Care, Reference Standards<br />
<strong>Abstract</strong> : Background. The Worldwide Antimalarial Resistance Network (WWARN) is a global collaboration to support the objective that<br />
anyone affected by malaria receives effective and safe drug treatment. The Pharmacology module aims to inform optimal<br />
anti-malarial drug selection. There is an urgent need to define the drug exposure - effect relationship for most anti-malarial<br />
drugs. Few anti-malarials have had their therapeutic blood concentration levels defined. One <strong>of</strong> the main challenges in<br />
assessing safety and efficacy data in relation to drug concentrations is the comparability <strong>of</strong> data generated from different<br />
laboratories. To explain differences in anti-malarial pharmacokinetics in studies with different measurement laboratories it is<br />
necessary to confirm the accuracy <strong>of</strong> the assay methods. This requires the establishment <strong>of</strong> an external quality assurance<br />
process to assure results that can be compared. This paper describes this process. Methods. The pharmacology module <strong>of</strong><br />
WWARN has established a quality assurance/quality control (QA/QC) programme consisting <strong>of</strong> two separate components:. 1. A<br />
pr<strong>of</strong>iciency testing programme where blank human plasma spiked with certified reference material (CRM) in different<br />
concentrations is sent out to participating bioanalytical laboratories. 2. A certified reference standard programme where<br />
accurately weighed amounts <strong>of</strong> certified anti-malarial reference standards, metabolites, and internal standards are sent to<br />
participating bioanalytical and in vitro laboratories. Conclusion. The pr<strong>of</strong>iciency testing programme is designed as a<br />
cooperative effort to help participating laboratories assess their ability to carry out drug analysis, resolve any potential<br />
problem areas and to improve their results - and, in so doing, to improve the quality <strong>of</strong> anti-malarial pharmacokinetic data<br />
published and shared with WWARN. By utilizing the same source <strong>of</strong> standards for all laboratories, it is possible to minimize<br />
bias arising from poor quality reference standards. By providing anti-malarial drug standards from a central point, it is possible<br />
to lower the cost <strong>of</strong> these standards. © <strong>2010</strong> Lourens et al; licensee BioMed Central Ltd.<br />
No.726<br />
Author (s) : Lowanitchapat, A., Payungporn, S., Sereemaspun, A., Ekpo, P., Phulsuksombati, D., Poovorawan, Y., Chirathaworn, C.<br />
Title : Expression <strong>of</strong> TNF-α, TGF-β, IP-10 and IL-10 mRNA in kidneys <strong>of</strong> hamsters infected with pathogenic Leptospira<br />
Source : Comparative Immunology, Microbiology and Infectious Diseases, Volume 33, Issue 5, September <strong>2010</strong>, Pages 423-434<br />
Document Type : Article<br />
Keywords : IL-10; IP-10; Leptospirosis; TGF; TNF<br />
<strong>Abstract</strong> : Leptospirosis is a worldwide zoonosis caused by pathogenic Leptospira. Although several components <strong>of</strong> this organism have<br />
been identified, the molecular mechanisms underlying pathogenesis <strong>of</strong> this infectious disease are still poorly understood.<br />
Besides, direct injury by microbial factors, cytokines produced in response to infection have been proposed to be involved in<br />
pathogenesis <strong>of</strong> leptospirosis. In this study, cytokine gene expression in kidneys was investigated. Hamsters were injected with<br />
pathogenic Leptospira interrogans serovar Pyrogenes and were sacrificed on days 3, 5 and 7 after infection. RNA was extracted<br />
from kidney tissues. Real-time PCR was performed to demonstrate expression <strong>of</strong> TNF-α, TGF-β, IP-10 and IL-10 mRNA in<br />
kidneys.TNF-α, TGF-β and IP-10 expression could be demonstrated since day 3 post-infection whereas IL-10 expression was<br />
detected later on day 5. Leptospira infection resulted in not only expression <strong>of</strong> a proinflammatory cytokine, TNF-α, but also<br />
a T cell chemokine, IP-10. Detection <strong>of</strong> IP-10 suggested the involvement <strong>of</strong> T cell recruitment in the immune response or<br />
pathology in infected kidneys. Expressions <strong>of</strong> anti-inflammatory cytokines, TGF-β and IL-10 were also observed. However, the<br />
level <strong>of</strong> TGF-β expression was prominent since day 3 post-infection whereas IL-10 expression was clearly observed on day 5.<br />
Further experiments will provide additional information whether there is a correlation between the expression <strong>of</strong> these<br />
cytokines and pathologies found in an affected organ. © 2009 Elsevier Ltd.<br />
266
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.727<br />
Author (s) : Löwenberg, E.C., Charunwatthana, P., Cohen, S., Van Den Born, B.-J., Meijers, J.C.M., Yunus, E.B., Hassan, M.U., Hoque, G.,<br />
Maude, R.J., Nuchsongsin, F., Levi, M., Dondorp, A.M.<br />
Title : Severe malaria is associated with a deficiency <strong>of</strong> von Willebrand factor cleaving protease, ADAMTS13<br />
Source : Thrombosis and Haemostasis, Volume 103, Issue 1, January <strong>2010</strong>, Pages 181-187<br />
Document Type : Article<br />
Keywords : ADAMTS13; Falciparum malaria; Thrombotic microangiopathy; Thrombotic thrombocytopenic purpura; Von willebrand<br />
factor cleaving protease<br />
<strong>Abstract</strong> : Severe falciparum malaria remains a major killer in tropical countries. Central in the pathophysiology is mechanical<br />
obstruction in the microcirculation caused by cytoadherence and sequestration <strong>of</strong> parasitized erythrocytes. However, the<br />
pathogenesis <strong>of</strong> many features complicating severe malaria, including coma, renal failure and thrombocytopenia, remains<br />
incompletely understood. These disease manifestations are also key features <strong>of</strong> thrombotic thrombocytopenic purpura, a lifethreatening<br />
disease strongly associated with a deficiency <strong>of</strong> the von Willebrand factor (VWF) cleaving protease, ADAMTS13. We<br />
measured plasma ADAMTS13 activity, VWF antigen and VWF propeptide levels in 30 patients with severe falciparum malaria,<br />
12 patients with uncomplicated falciparum malaria and 14 healthy Bangladeshi controls. In patients with severe malaria<br />
ADAMTS13 activity levels were markedly decreased in comparison to normal controls (mean [95%CI]: 23% [20-26] vs. 64%<br />
[55-72]) and VWF antigen and propeptide concentrations were significantly elevated (VWF antigen: 439% [396-481] vs. 64%<br />
[46-83]; VWF propeptide: 576% [481-671] vs. 69% [59-78]). In uncomplicated malaria VWF levels were also increased<br />
compared to healthy controls but ADAMTS13 activity was normal. The results suggest that decreased ADAMTS13 activity in<br />
combination with increased VWF concentrations may contribute to the complications in severe malaria. © Schattauer <strong>2010</strong>.<br />
No.728<br />
Author (s) : Lu, Y., Xiao, P., Shao, Y., Zhang, G., Thanyasiriwat, T., Bao, J.<br />
Title : Development <strong>of</strong> new markers to genotype the functional SNPs <strong>of</strong> SSIIa, a gene responsible for gelatinization temperature <strong>of</strong><br />
rice starch<br />
Source : Journal <strong>of</strong> Cereal Science, Volume 52, Issue 3, November <strong>2010</strong>, Pages 438-443<br />
Document Type : Article<br />
Keywords : Gelatinization temperature; Rice; Single nucleotide polymorphism; SSIIa<br />
<strong>Abstract</strong> : Gelatinization temperature (GT) is an important quality predictor that determines the cooking quality <strong>of</strong> rice. GT is genetically<br />
controlled by the starch synthase IIa (SSIIa) gene. Two functional single nucleotide polymorphisms (SNPs) inside the SSIIa have<br />
already been found to be responsible for the variation <strong>of</strong> GT. One <strong>of</strong> these, GC/TT SNP at 4329/4330 bp, could be genotyped<br />
by four primers in a single PCR (Bao et al., 2006a), but another one, G/A SNP at 4198 bp, has not been detected by a PCRbased<br />
marker. Here, we developed cleaved amplified polymorphic sequence (CAPS) and derived cleaved amplified<br />
polymorphic sequence (dCAPS) markers to detect these SNPs. A dCAPS marker that the PCR products were cleaved by the<br />
BseR I restriction endonuclease was designed to detect GC/TT SNP. Both CAPS and dCAPS markers were designed to detect<br />
G/A SNP using the restriction endonuclease Nla III and Tsp45 I, respectively. All the markers developed were co-dominant. It<br />
was known that the A allele <strong>of</strong> G/A SNP was rare among rice germplasm, but it was still in use by rice breeders. 11 rice<br />
accessions including landrace and breeding lines with A allele <strong>of</strong> G/A SNP were detected. The F2 individuals from two crosses<br />
were used to analyze the co-segregation between the SNP alleles and the GT. The segregation ratio <strong>of</strong> two SNPs did not<br />
conform to the expected Mendelian ratio <strong>of</strong> 1:2:1, but the SNPs were co-segregated with GT. The markers developed in the<br />
present study would be useful in molecular breeding for the improvement <strong>of</strong> the quality <strong>of</strong> rice grain. © <strong>2010</strong> Elsevier Ltd.<br />
No.729<br />
Author (s) : Lubell, Y.<br />
Title : Cost-effective use <strong>of</strong> prereferral treatment for severe malaria<br />
Source : The Lancet, Volume 376, Issue 9756, 4 December <strong>2010</strong>, Pages 1880-1881<br />
Document Type : Note<br />
Keywords : antibiotic agent, artesunate; antibiotic therapy, cost effectiveness analysis, drug cost, drug efficacy, health care access, health<br />
program, malaria, note, priority journal; Administration, Rectal, Antimalarials, Artemisinins, Community Networks, Cost-Benefit<br />
Analysis, Delivery <strong>of</strong> Health Care, Endemic Diseases, Health Services Accessibility, Humans, Malaria<br />
<strong>Abstract</strong> : –<br />
267
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.730<br />
Author (s) : Lubell, Y., Mills, A.J., Whitty, C.J.M., Staedke, S.G.<br />
Title : An economic evaluation <strong>of</strong> home management <strong>of</strong> malaria in uganda: An interactive markov model<br />
Source : PLoS ONE, Volume 5, Issue 8, <strong>2010</strong>, Article number e12439<br />
Document Type : Article<br />
Keywords : amodiaquine plus artesunate, artemether plus benflumetol, chloroquine, dihydroartemisinin plus<br />
piperaquine, fansidar, antimalarial agent; article, cost effectiveness analysis, disability, disease severity, drug cost, drug<br />
efficacy, economic evaluation, fever, health care facility, health service, hidden Markov model, home<br />
care, human, malaria, probability, Uganda, cost benefit analysis, drug combination, economics, manpower, nursing, preschool<br />
child, theoretical model; Antimalarials, Child, Preschool, Cost-Benefit Analysis, Drug Combinations, Home Care<br />
Services, Humans, Malaria, Markov Chains, Models, Theoretical, Uganda<br />
<strong>Abstract</strong> : Background: Home management <strong>of</strong> malaria (HMM), promoting presumptive treatment <strong>of</strong> febrile children in the community, is<br />
advocated to improve prompt appropriate treatment <strong>of</strong> malaria in Africa. The cost-effectiveness <strong>of</strong> HMM is likely to vary<br />
widely in different settings and with the antimalarial drugs used. However, no data on the cost-effectiveness <strong>of</strong> HMM<br />
programmes are available. Methods/Principal Findings: A Markov model was constructed to estimate the cost-effectiveness <strong>of</strong><br />
HMM as compared to conventional care for febrile illnesses in children without HMM. The model was populated with data<br />
from Uganda, but is designed to be interactive, allowing the user to adjust certain parameters, including the antimalarials<br />
distributed. The model calculates the cost per disability adjusted life year averted and presents the incremental costeffectiveness<br />
ratio compared to a threshold value. Model output is stratified by level <strong>of</strong> malaria transmission and the<br />
probability that a child would receive appropriate care from a health facility, to indicate the circumstances in which HMM is<br />
likely to be cost-effective. The model output suggests that the cost-effectiveness <strong>of</strong> HMM varies with malaria transmission,<br />
the probability <strong>of</strong> appropriate care, and the drug distributed. Where transmission is high and the probability <strong>of</strong> appropriate<br />
care is limited, HMM is likely to be cost-effective from a provider perspective. Even with the most effective antimalarials,<br />
HMM remains an attractive intervention only in areas <strong>of</strong> high malaria transmission and in medium transmission areas with a<br />
lower probability <strong>of</strong> appropriate care. HMM is generally not cost-effective in low transmission areas, regardless <strong>of</strong> which<br />
antimalarial is distributed. Considering the analysis from the societal perspective decreases the attractiveness <strong>of</strong> HMM.<br />
Conclusion: Syndromic HMM for children with fever may be a useful strategy for higher transmission settings with limited<br />
health care and diagnosis, but is not appropriate for all settings. HMM may need to be tailored to specific settings, accounting<br />
for local malaria transmission intensity and availability <strong>of</strong> health services. © <strong>2010</strong> Lubell et al.<br />
No.731<br />
Author (s) : Luealamai, S., Panijpan, B., Ruenwongsa, P.<br />
Title : Using computer simulation and game module to enhance undergraduate students' 3D-visualization<br />
Source : <strong>International</strong> Journal <strong>of</strong> Learning, Volume 17, Issue 5, <strong>2010</strong>, Pages 355-370<br />
Document Type : Article<br />
Keywords : 3D Computer Module; Crystal Lattice; Undergraduate Students; Unit Cell; Visualization<br />
<strong>Abstract</strong> : This study aimed to enhance students' visualization <strong>of</strong> the micro-scale structure <strong>of</strong> the solid crystal using computer<br />
simulation and game for undergraduate science students. A computer teaching module, which comprised 3D models, various<br />
computer simulations and an instructional game, was developed. Two groups <strong>of</strong> students participated in the learning unit: the<br />
traditional group was taught in the traditional lecture format, whereas the experimental group received instructions that<br />
incorporated the computer module activities. Our research instruments consisted <strong>of</strong> a test, a rating scale questionnaire and<br />
personal interviews. Both groups took the pretest and posttest. The research results indicated that students who were<br />
supplemented with the computer module improved their knowledge and visualization ability <strong>of</strong> the micro- structure <strong>of</strong> solid<br />
crystals more than the traditional group. © Common Ground, Sutha Luealamai, Bhinyo Panijpan, Pintip Ruenwongsa.<br />
No.732<br />
Author (s) : Luksamijarulkul, P., Kongtip, P.<br />
Title : Microbial counts and particulate matter levels in roadside air samples under skytrain stations, Bangkok, Thailand<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 3, May <strong>2010</strong>, Pages 678-684<br />
Document Type : Article<br />
Keywords : Air quality; Microbial counts; Particulate matter levels; Roadside air samples<br />
268
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : In conditions with heavy traffic and crowds <strong>of</strong> people on roadside areas under skytrain stations in Bangkok, the natural air<br />
ventilation may be insufficient and air quality may be poor. A study <strong>of</strong> 350 air samples collected from the road-side, under<br />
skytrain stations in Bangkok, was carried out to assess microbial counts (210 air samples) and particulate matter (PM10) levels<br />
(140 samples). The results reveal the mean ± standard deviation bacterial counts and fungal counts were 406.8±302.7 cfu/m3<br />
and 128.9±89.7 cfu/m3, respectively. The PM10 level was 186.1±188.1 μg/m3. When compared to recommended levels,<br />
4.8% <strong>of</strong> air samples (10/210 samples) had bacterial counts more than recommended levels (>1,000 cfu/ m3) and 27.1%<br />
(38/140 samples) had PM10 levels more than recommended levels (>120 μg/m3). These may affect human health,<br />
especially <strong>of</strong> street venders who spend most <strong>of</strong> their working time in these areas.<br />
No.733<br />
Author (s) : Lulitanond, A., Chanawong, A., Sribenjalux, P., Wilailuckana, C., Kaewkes, W., Vorachit, M., Ito, T., Hiramatsu, K.<br />
Title : Preliminary report <strong>of</strong> SCCmec-types and antimicrobial susceptibilities <strong>of</strong> methicillin-resistant Staphylococcus aureus isolates<br />
from a university hospital in Thailand<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 4, July <strong>2010</strong>, Pages 920-927<br />
Document Type : Article<br />
Keywords : Antimicrobial susceptibility; MRSA; SCCmec; Thailand<br />
<strong>Abstract</strong> : Methicillin-resistant Staphylococcus aureus (MRSA) has spread worldwide. It is a major cause <strong>of</strong> hospital-acquired infections in<br />
most hospitals for nearly half century. The present study was conducted to examine the antimicrobial susceptibilities and<br />
staphylococcal cassette chromosome mec (SCCmec)-type for MRSA isolates from 237 patients treated at Srinagarind Hospital<br />
between September 2002 and August 2003. Antimicrobial susceptibility testing for all isolates was performed using an agar<br />
dilution method and SCCmec-types <strong>of</strong> 81 representatives from 237 isolates were determined using multiplex PCR. The<br />
minimum inhibitory concentration (MIC) ranges for the MRSA isolates were as follows: cefazolin 8 to ≥ 64; erythromycin ≤ 0.5<br />
to ≥ 64; gentamicin ≤ 0.5 to ≥ 64; imipenem ≤ 0.5 to > 16; <strong>of</strong>loxacin ≤ 0.5 to ≥ 64; oxacillin 16 to ≥ 64; tetracycline 2 to ≥ 64<br />
and vancomycin ≤ 0.5 to 2 μg/ml. All MRSA isolates were susceptible to vancomycin, but only 0.4% to 8.9% was susceptible<br />
to the remaining antimicrobial agents. Of the 81 isolates tested, 2 types <strong>of</strong> SCCmec were found (76 with type III and 2 with<br />
type II) and no mecA gene was detected in 3 isolates. Sixty-seven <strong>of</strong> the 78 isolates carried the mercury-resistant operon. The<br />
multilocus sequence type in isolates with type III SCCmec was ST239 and in isolates with type II SCCmec was ST5.<br />
No.734<br />
Author (s) : Lundberg, P.C., Kerdonfag, P.<br />
Title : Spiritual care provided by Thai nurses in intensive care units<br />
Source : Journal <strong>of</strong> Clinical Nursing, Volume 19, Issue 7-8, April <strong>2010</strong>, Pages 1121-1128<br />
Document Type : Article<br />
Keywords : Intensive care; Nurses; Nursing; Spiritual care; Thailand<br />
<strong>Abstract</strong> : Aim: The aim <strong>of</strong> this study was to explore how Thai nurses in intensive care units <strong>of</strong> a university hospital in Bangkok provided<br />
spiritual care to their patients. Background: The function <strong>of</strong> nursing is to promote health, prevent illness, restore health and<br />
alleviate suffering. An holistic approach to this promotion includes spirituality. Design: An explorative qualitative study was<br />
used. Method: Thirty Thai nurses, selected through purposive sampling with the snowball technique, participated voluntarily.<br />
Semi-structured interviews with open-ended questions were carried out, taped-recorded, transcribed verbatim and subjected<br />
to content analysis. Results: Five themes related to the provision <strong>of</strong> spiritual care emerged: giving mental support, facilitating<br />
religious rituals and cultural beliefs, communicating with patients and patients' families, assessing the spiritual needs <strong>of</strong><br />
patients and showing respect and facilitating family participation in care. Several ways <strong>of</strong> improving the spiritual care were<br />
suggested by the nurses. Conclusions: Spirituality was an important part <strong>of</strong> the care for the nurses when meeting the needs <strong>of</strong><br />
their patients and the patients' families. Therefore, nursing education should enhance nurses' understanding and awareness <strong>of</strong><br />
spiritual issues and prepare them to respond to human spiritual needs. Relevance to clinical practice: Nurses should consider<br />
spirituality as an important component <strong>of</strong> holistic care. During their pr<strong>of</strong>essional career, they should expand their knowledge<br />
and understanding <strong>of</strong> spirituality and develop tools for assessment <strong>of</strong> spiritual needs. © <strong>2010</strong> The Authors. Journal<br />
compilation © <strong>2010</strong> Blackwell Publishing Ltd.<br />
No.735<br />
Author (s) : Luppanapornlarp S., Leelataweewud P., Putongkam P., Ketanont S.<br />
Title : Periodontal status and orthodontic treatment need <strong>of</strong> autistic children.<br />
269
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Source : World Journal <strong>of</strong> Orthodontics, <strong>2010</strong>, Volume: 11, Issue: 3, Pages: 256-261<br />
Document Type : Article<br />
Keywords : –<br />
<strong>Abstract</strong> : AIM: To evaluate the periodontal status and orthodontic treatment need <strong>of</strong> autistic children and compare these findings to<br />
nonaffected, same-age individuals. METHOD: The periodontal status and orthodontic treatment need were evaluated in 32<br />
autistic and 48 nonautistic boys and girls age 8 years to 12 years (mean 9.7 ± 1.2 years and 9.9 ± 1.1 years, respectively). The<br />
periodontal status <strong>of</strong> all subjects was recorded using the Community Periodontal Index <strong>of</strong> Treatment Need (CPITN) with a<br />
slight modification. The orthodontic treatment need was determined using the Dental Aesthetic Index (DAI). Chi-square test<br />
and odds ratio were used for statistical analysis. RESULTS: No significant sex differences were found in the autistic or<br />
nonautistic groups. The autistic children presented with a significantly poorer periodontal status than the nonautistic children<br />
(P.05);<br />
however, children with autism showed missing teeth, spacing, diastemas, reverse overjets, open bites, and Class II molar<br />
relationship tendencies in a higher percentage than nonautistic individuals. In all, autistic children and nonautistic children<br />
frequently needed orthodontic treatment. CONCLUSION: This study suggests that children with autism require special dental<br />
management to improve their oral hygiene as well as their dental esthetics. More care from parents, general dentists, and<br />
pedodontists/orthodontists should be provided routinely to autistic children. © <strong>2010</strong> BY QUINTESSENCE PUBLISHING CO, INC.<br />
No.736<br />
Author (s) : Luppanapornlarp, S., Kajii, T.S., Surarit, R., Iida, J.<br />
Title : Interleukin-1β levels, pain intensity, and tooth movement using two different magnitudes <strong>of</strong> continuous orthodontic force<br />
Source : European Journal <strong>of</strong> Orthodontics, Volume 32, Issue 5, October <strong>2010</strong>, Pages 596-601<br />
Document Type : Article<br />
Keywords : interleukin 1beta; adolescent, adult, article, canine tooth, comparative study, female, gingivitis, human, instrumentation, male,<br />
malocclusion, mechanical stress, metabolism, orthodontic device, orthodontics, pain, periodontal disease; Adolescent,<br />
Cuspid, Female, Gingival Crevicular Fluid, Humans, Interleukin-1beta, Male, Malocclusion, Angle Class I, Orthodontic Appliance<br />
Design, Orthodontic Wires, Pain, Stress, Mechanical, Tooth Movement, Young Adult<br />
<strong>Abstract</strong> : This study aimed to determine the optimum orthodontic force from a broader perspective. Interleukin (IL)-1β levels in<br />
human gingival crevicular fluid (GCF), pain intensity, and the amount <strong>of</strong> tooth movement were measured during canine<br />
retraction using different magnitudes <strong>of</strong> continuous orthodontic force.Sixteen subjects (two males and 14 females), aged 18-<br />
24 years, diagnosed with Class I bimaxillary protrusion and treated with first premolar extractions participated in this study.<br />
The upper canines were retracted with continuous forces <strong>of</strong> 50 or 150 g using nickel-titanium coil springs on segmented<br />
archwires. One <strong>of</strong> the lower canines was used as a control. GCF was collected from the distal site <strong>of</strong> each tooth at specific<br />
time points. IL-1β concentrations, pain intensity, using the visual analogue scale (VAS), and the amount <strong>of</strong> tooth movement<br />
were evaluated. One-way analysis <strong>of</strong> variance, Friedman, and paired t-tests were used for comparisons <strong>of</strong> IL-1β in GCF, the<br />
plaque and gingival indices, and the efficiency <strong>of</strong> tooth movement on pain perception, respectively.IL-1β concentration in<br />
the 150 g group showed the highest level at 24 hours and 2 months with significant differences compared with the control<br />
group (P < 0.05). The mean VAS score <strong>of</strong> pain intensity from the 150 g force was significantly greater than from the 50 g force<br />
at 24 hours (P < 0.01). However, no significant difference in the amount <strong>of</strong> tooth movement was found between these two<br />
different magnitudes <strong>of</strong> continuous force at 2 months. A 50 g force could effectively induce tooth movement similar to 150 g<br />
with less pain and less inflammation. © The Author <strong>2010</strong>.<br />
No.737<br />
Author (s) : Lwin, KM., Zwang, J., Phyo, AP., Ohn, M., Proux, S., Leimanis, M., Tarning, J., Lindegardh, N., Cheah, P., Singhasivanon, P., White,<br />
NJ., Nosten, F.<br />
Title : A RANDOMISED, DOUBLE-BLIND PLACEBO CONTROLLED TRIAL OF TWO DIFFERENT DOSING REGIMENS OF DIHYDROARTEMISININ-<br />
PIPERAQUINE FOR INTERMITTENT PREVENTIVE TREATMENT OF ADULTS AT HIGH RISK OF MALARIA IN THAILAND<br />
Source : AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, Volume: 83, Issue: 5, Pages: 372-372, Supplement: S, Published:<br />
NOV <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
270
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.738<br />
Author (s) : Maek-a-nantawat, W.; Huntrup, A.; Malakul, K.; Silachamroon, U.<br />
Title : Atopic Diseases Share the Cake <strong>of</strong> Association with Chronic Urticaria<br />
Source : JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, Volume: 125, Issue: 2, Supplement: 1, Pages: AB94-AB94, Published: FEB<br />
<strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.739<br />
Author (s) : Maguire, J.; Thakkinstian, A.; Levi, C.; Lincz, L.; Bissett, L.; Sturm, J.; Scott, R.; Attia, J.<br />
Title : Genetic Influences on Ischemic Stroke 90-day Functional Outcome: a Novel Association<br />
Source : CIRCULATION, Volume: 122, Issue: 2, Pages: E175-E175, Meeting <strong>Abstract</strong>: P368, Published: JUL 13 <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.740<br />
Author (s) : Mahachoklertwattana, P., Suthutvoravut, U., Poomthavorn, P., Charoenkiatkul, S., Udomsubpayakul, U., Rajatanavin, R.<br />
Title : Sexual maturation in Thai boys<br />
Source : Journal <strong>of</strong> Pediatric Endocrinology and Metabolism, Volume 23, Issue 1-2, January <strong>2010</strong>, Pages 65-71<br />
Document Type : Article<br />
Keywords : Puberty; Sexual maturation<br />
<strong>Abstract</strong> : Background: A worldwide secular trend towards earlier onset <strong>of</strong> puberty in girls has been noted during recent years. However,<br />
the data on sexual maturation <strong>of</strong> boys are relatively scarce and normative data <strong>of</strong> sexual maturation in Thai boys are still<br />
lacking. Aim: To determine the age <strong>of</strong> secondary sexual maturation in normal Thai boys. Methods: Three hundred healthy<br />
urban boys aged 9-18 years were recruited during January 1997 to December 1999. Genital and pubic hair maturity staging<br />
was determined using the method <strong>of</strong> Marshall and Tanner. Testicular size was assessed by Prader orchidometer. Probit<br />
analysis was used to analyze the onset <strong>of</strong> puberty (gonadarche) and pubarche. Results: Median (range) ages <strong>of</strong> the onset <strong>of</strong><br />
puberty and pubarche were 10.8 (9.5-12) and 12.4 (10.9-13.9) years, respectively. Conclusion: The age <strong>of</strong> onset <strong>of</strong> genital<br />
development in boys living in Bangkok seems to be slightly earlier than that <strong>of</strong> boys in other countries. However, the onset <strong>of</strong><br />
pubic hair development is comparable. © Freund Publishing House Ltd.<br />
No.741<br />
Author (s) : Mahanam, T.; Sangdee, A.; Govitrapong, P.; Tongjaroenbuangam, W.<br />
Title : MELATONIN IMPROVES LEARNING AND MEMORY PERFORMANCES IMPAIRED BY DEXAMETHASONE-INDUCED STRESS MICE<br />
Source : JOURNAL OF NEUROCHEMISTRY, Volume: 115, Supplement: 1, Pages: 76-77, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.742<br />
Author (s) : Mahasaksawat, N.; Mukda, S.; Wongchitrat, P.; Govitrapong, P.<br />
Title : AMPHETAMINE-INDUCED CHANGE IN CIRCADIAN RHYTHMS OF DOPAMINERGIC SYSTEM IN RAT STRIATUM<br />
Source : JOURNAL OF NEUROCHEMISTRY, Volume: 115, Supplement: 1, Pages: 82-82, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.743<br />
Author (s) : Mahittikorn, A., Wickert, H., Sukthana, Y.<br />
271
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Toxoplasma gondii: Simple duplex RT-PCR assay for detecting SAG1 and BAG1 genes during stage conversion in<br />
immunosuppressed mice<br />
Source : Experimental Parasitology, Volume 124, Issue 2, February <strong>2010</strong>, Pages 225-231<br />
Document Type : Article<br />
Keywords : Duplex RT-PCR; Mice; Stage conversion; Toxoplasma gondii<br />
<strong>Abstract</strong> : Toxoplasmic encephalitis (TE) is caused by reactivation <strong>of</strong> dormant bradyzoites into rapidly dividing tachyzoites <strong>of</strong> the<br />
apicomplexan parasite Toxoplasma gondii in immune-compromised hosts. Diagnosis <strong>of</strong> this life-threatening disease is<br />
complicated, since it is difficult to distinguish between these two stages. It is, therefore, mainly based on a test positive for T.<br />
gondii antibodies, and specific clinical symptoms. We developed a duplex RT-PCR to detect the expression <strong>of</strong> bradyzoite<br />
(BAG1) and tachyzoite (SAG1) specific genes simultaneously during tachyzoite/bradyzoite stage conversion. The conversion<br />
reaction was observed in many organs <strong>of</strong> experimental mice, indicated by tachyzoites in the cerebrum, cerebellum, heart and<br />
lung, beginning in week 1 after the suppression period, and continuing until the end. Bradyzoites were also detected in nearly<br />
all organs throughout the study, suggesting that during the reactivation period, bradyzoites not only escape from cysts and<br />
reinvade neighboring cells as tachyzoites, but are also driven into developing new bradyzoites. The results <strong>of</strong> our study show<br />
that duplex RT-PCR is an easy, rapid, sensitive, and reproducible method, which is particularly valuable when numerous<br />
samples must be analyzed. This technique may usefully serve as an alternate tool for diagnosing TE in severely<br />
immunocompromised patients. © 2009 Elsevier Inc. All rights reserved.<br />
No.744<br />
Author (s) : Maingat, F., Viappiani, S., Zhu, Y., Vivithanaporn, P., Ellestad, K.K., Holden, J., Silva, C., Power, C.<br />
Title : Regulation <strong>of</strong> lentivirus neurovirulence by lipopolysaccharide conditioning: Suppression <strong>of</strong> CXCL10 in the brain by IL-10<br />
Source : Journal <strong>of</strong> Immunology, Volume 184, Issue 3, 1 February <strong>2010</strong>, Pages 1566-1574<br />
Document Type : Article<br />
Keywords : CD3 antigen, gamma interferon inducible protein 10, granzyme A, lipopolysaccharide, recombinant interleukin 10, interleukin<br />
10, neuroprotective agent; animal experiment, animal model, animal tissue, article, brain, cognition, controlled study, Feline<br />
immunodeficiency virus, gene expression, genetic analysis, human, human cell, Human immunodeficiency virus<br />
infection, human tissue, immunocytochemistry, immunosuppressive treatment, innate immunity, Lentivirinae, Lentivirus<br />
infection, lymphocytic infiltration, macrophage, monocyte, nerve cell, nonhuman, priority journal, real time polymerase chain<br />
reaction, supernatant, T lymphocyte, virus cell interaction, virus load, virus virulence, animal, cat, cell culture, cell<br />
motion, drug antagonism, growth, development and aging, Human immunodeficiency virus<br />
1, immunology, pathogenicity, pathology, physiology, tumor cell line, virology, virulence; Animals, Brain, Cats, Cell Line,<br />
Tumor, Cell Movement, Cells, Cultured, Chemokine CXCL10, HIV Infections, HIV-1, Humans, Immunodeficiency Virus,<br />
Feline, Interleukin-10, Lentivirus Infections, Lipopolysaccharides, Neurons, Neuroprotective Agents, Viral Load, Virulence<br />
<strong>Abstract</strong> : Lentivirus infections including HIV and feline immunodeficiency virus (FIV) cause neurovirulence, which is largely mediated by<br />
innate immunity. To investigate the interactions between neurovirulence and repeated conditioning by innate immune<br />
activation, models <strong>of</strong> lentivirus infection were exposed to LPS. Gene expression in HIV-infected (HIV+) and control (HIV-)<br />
patient brains was compared by real time RT-PCR and immunocytochemistry. Supernatants from mock and HIV-infected<br />
monocyte-derived macrophages exposed to LPS were applied to human neurons. FIV-infected (FIV+) and control (FIV-)<br />
animals were exposed repeatedly to LPS postinfection together with concurrent neurobehavioral testing, viral load, and host<br />
gene analyses. Brains from HIV+ individuals exhibited induction <strong>of</strong> CD3ε, CXCL10, and granzyme A expression (p < 0.05).<br />
Supernatants from HIV+ monocyte-derived macrophages induced CXCL10 expression in neurons, which was diminished by IL-<br />
10 treatment (p < 0.05). LPS-exposed FIV+ animals demonstrated lower plasma and brain viral loads (p < 0.05). Neuronal<br />
CXCL10 expression was increased in FIV+ animals but was suppressed by LPS exposure, together with reduced brain CD3ε<br />
and granzyme A expression (p < 0.05). In conjunction with preserved NeuN-positive neuronal counts in parietal cortex (p <<br />
0.05), FIV+ animals exposed to LPS also showed less severe neurobehavioral deficits (p < 0.05). Repeated LPS exposures<br />
suppressed CXCL10 in the brain and ensuing T cell infiltration with a concomitant reduction in neurovirulence. Thus, innate<br />
immune chronic conditioning exerted beneficial effects on neurovirulence through suppression <strong>of</strong> a specific chemotactic<br />
factor, CXCL10, mediated by IL-10, leading to reduced leukocyte infiltration and release <strong>of</strong> neurotoxic factors. Copyright ©<br />
<strong>2010</strong> by The American Association <strong>of</strong> Immunologists, Inc.<br />
No.745<br />
Author (s) : Maksymowych, W.P.; Chiowchanwisawakit, P.; Lambert, R.G.W.<br />
272
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : TNF BLOCKING AGENTS PROMOTE RESOLUTION OF EROSIONS IN PATIENTS WITH SPONDYLOARTHRITIS<br />
Source : CLINICAL AND EXPERIMENTAL RHEUMATOLOGY, Volume: 28, Issue: 4, Pages: 636-636, Published: JUL-AUG <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.746<br />
Author (s) : Malaithong, N., Polsomboon, S., Poolprasert, P., Parbaripai, A., Bangs, M.J., Suwonkerd, W., Pothikasikorn, J., Akratanakul, P.,<br />
Chareonviriyaphap, T.<br />
Title : Human-landing patterns <strong>of</strong> anopheles dirus sensu lato (Diptera: Culicidae) in experimental huts treated with DDT or<br />
deltamethrin<br />
Source : Journal <strong>of</strong> Medical Entomology, Volume 47, Issue 5, September <strong>2010</strong>, Pages 823-832<br />
Document Type : Article<br />
Keywords : Anopheles dirus; behavioral response; DDT; deltamethrin; experimental hut<br />
<strong>Abstract</strong> : Anopheles dirus females landing on humans inside experimental huts treated with residual applications <strong>of</strong> DDT or<br />
deltamethrin were observed during the wet season in Pu Teuy Village, Kanchanaburi Province, western Thailand. Two<br />
identical experimental huts were constructed in the fashion <strong>of</strong> typical local rural Thai homes. Pretreatment (baseline) humanlanding<br />
collections (HLC) in both huts showed an early evening peak <strong>of</strong> activity between 1900 and 2000 h with no significant<br />
difference in numbers <strong>of</strong> mosquitoes captured between huts over a period <strong>of</strong> 30 collection nights. During posttreatment HLC,<br />
female mosquitoes continued to show greater landing activity inside huts fitted with insecticide-treated panels during the first<br />
half <strong>of</strong> the evening compared with the second half. A greater number (proportion) <strong>of</strong> An. dims females landed on humans in<br />
the hut treated with deltamethrin compared with DDT. Comparing pre- and posttreatment HLC, the DDT-treated hut showed<br />
a 79.4% decline in attempted blood feeding, whereas exposure to deltamethrin resulted in a 56.3% human-landing<br />
reduction. An odds ratio was performed to demonstrate the relative probability (risk) <strong>of</strong> mosquitoes entering and attempting<br />
to blood feed in the two treated huts compared with untreated control huts. Mosquitoes were ≈times less likely to land on<br />
humans inside a DDT-treated hut compared with the deltamethrin-treated hut. Although both chemicals exerted strong<br />
excitatory responses, DDT appears to have a more pronounced and significant (P = 0.002) effect on behavior than<br />
deltamethrin, resulting in greater movement away from the insecticide source and thus potential reduction <strong>of</strong> blood-feeding<br />
activity. © <strong>2010</strong> Entomological Society <strong>of</strong> America.<br />
No.747<br />
Author (s) : Malisorn, N., Morales, N.P., Sanvarinda, Y., Wanikiat, P.<br />
Title : 17β-estradiol attenuates LPS-induced interleukin-8 production by human peripheral blood monocytes through estrogen<br />
receptor-αactivation<br />
Source : African Journal <strong>of</strong> Pharmacy and Pharmacology, Volume 4, Issue 11, November <strong>2010</strong>, Pages 806-810<br />
Document Type : Article<br />
Keywords : Estrogen receptor; Interleukin 8; Monocytes<br />
<strong>Abstract</strong> : Proper regulation <strong>of</strong> the immune response is essential for immune homeostasis. Several proinflammatory cytokines released<br />
from activated monocytes mediate inflammation, including interleukine-8 (IL-8) which recruits neutrophils to the site <strong>of</strong><br />
inflammation. 17β-Estradiol (E2) has a direct role in the modulation <strong>of</strong> the innate immune function and mediates pr<strong>of</strong>ound<br />
effects on immune function <strong>of</strong> the monocytes. The effects <strong>of</strong> 17β-E2 are mediated principally by two receptor subtypes, ER<br />
and ER; both are expressed in monocytes. The aim <strong>of</strong> this study was, therefore, to characterize the estrogen receptor<br />
subtypes that mediate the estrogen effects on LPS-activated IL-8 production by human peripheral blood monocytes. 17β-E2<br />
and PPT attenuated the production <strong>of</strong> IL-8 by LPS-activated monocytes in a dose-dependent manner and these effects can<br />
be reversed by ICI182, 780. These results suggested a role <strong>of</strong> ER on the attenuating effect <strong>of</strong> 17β-E2 on IL-8 production by<br />
human peripheral blood monocytes. © <strong>2010</strong> Academic Journals.<br />
No.748<br />
Author (s) : Maltezos, G., Johnston, M., Taganov, K., Srichantaratsamee, C., Gorman, J., Baltimore, D., Chantratita, W., Scherer, A.<br />
Title : Exploring the limits <strong>of</strong> ultrafast polymerase chain reaction using liquid for thermal heat exchange: A pro<strong>of</strong> <strong>of</strong> principle<br />
Source : Applied Physics Letters, Volume 97, Issue 26, 27 December <strong>2010</strong>, Article number 264101<br />
Document Type : Article<br />
273
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : Avian influenza, Base pairs, Chemical systems, Clinical settings, E.coli O157:H7, Human immunodeficiency virus, Limiting<br />
factors, PCR amplification, Pro<strong>of</strong> <strong>of</strong> principles, Real-time polymerase chain reaction, Shiga toxin, State-<strong>of</strong>-the-art<br />
devices, Thermal exchange, Thermal heat, Thermal ramp rates, Thermococcus kodakaraensis, Traditional devices, Ultra-fast;<br />
Escherichia coli, Liquids, Metal testing, Polymerization, Polymers, Viruses; Polymerase chain reaction<br />
<strong>Abstract</strong> : Thermal ramp rate is a major limiting factor in using real-time polymerase chain reaction (PCR) for routine diagnostics. We<br />
explored the limits <strong>of</strong> speed by using liquid for thermal exchange rather than metal as in traditional devices, and by testing<br />
different polymerases. In a clinical setting, our system equaled or surpassed state-<strong>of</strong>-the-art devices for accuracy in amplifying<br />
DNA/RNA <strong>of</strong> avian influenza, cytomegalovirus, and human immunodeficiency virus. Using Thermococcus kodakaraensis<br />
polymerase and optimizing both electrical and chemical systems, we obtained an accurate, 35 cycle amplification <strong>of</strong> an 85base<br />
pair fragment <strong>of</strong> E. coli O157:H7 Shiga toxin gene in as little as 94.1 s, a significant improvement over a typical 1 h PCR<br />
amplification. © <strong>2010</strong> American Institute <strong>of</strong> Physics.<br />
No.749<br />
Author (s) : Manatsathit, W., Sermsathanasawadi, R., Wongjittraporn, S., Chavalitdhamrong, D., Kijsirichareanchai, K., Manuyakorn, A.,<br />
Pongprasobchai, S.<br />
Title : A Case Series <strong>of</strong> Eosinophilic Gastroenteritis and Eosinophilic Colitis in Thailand<br />
Source : AMERICAN JOURNAL OF GASTROENTEROLOGY, Volume: 105, Supplement: 1, Pages: S76-S76, Meeting <strong>Abstract</strong>: 202, Published:<br />
OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.750<br />
Author (s) : Mandee, S., Siriussawakul, A., Suraseranivongse, S., Khanwilai, J., Nitigarun, P.<br />
Title : Time duration to safety sitting in parturient receiving spinal anesthesia for cesarean section with 0.5% Bupivacaine and<br />
morphine<br />
Source : ASIAN BIOMEDICINE, Volume: 4, Issue: 3, Pages: 485-489, Published: JUN <strong>2010</strong><br />
Document Type : Article<br />
Keywords : Ambulation; Cesarean section; Spinal anesthesia<br />
<strong>Abstract</strong> : Background: Spinal anesthesia has been used for cesarean section for a long time. However, the proportion <strong>of</strong> post-cesarean<br />
paturients who were able to sit at the fourth hour still remains unclear. Objective: Investigate the proportion <strong>of</strong> post-cesarean<br />
paturients that were able to sit at the fourth hour following spinal anesthesia with 0.5% hyperbaric bupivacaine and<br />
morphine. Furthermore, investigate the optimum time to encourage ambulation, and the risk factors delaying time duration<br />
to sit. Methods: A prospective observational study was conducted in 240 patients with American Society <strong>of</strong> Anesthesiologists<br />
physical status classification I and II, and single pregnancy parturients undergoing cesarean section. The patients who had<br />
body mass index (BMI) >35, estimated blood loss >1000 mL, needed postoperative bed rest, or received postoperative<br />
sedation were excluded. Hyperbaric bupivacaine 8-11 mg and morphine 0.2-0.3 mg were used. The patients were evaluated<br />
at the fourth hour until they could sit without adverse events or complete the sixth hour. All patients were evaluated for risk<br />
factors delaying the time duration to sit. Results: Out <strong>of</strong> 240 patients, 77.0%, 90.9%, and 98.4% were able to sit at the fourth,<br />
fifth, and sixth hour, respectively. The risk factors that delayed time to sit were Bromage scale >1 and pain score >3 by the<br />
univariate analysis, and were Bromage scale >1 and pain score >3 by the multivariate analysis. Conclusion: Seventy-seven<br />
percent <strong>of</strong> the patients could sit at the fourth hour, and most patients (98%) could sit at the sixth hour. The risk factors that<br />
delayed the time duration to sit were Bromage score >1 and pain score >3.<br />
No.751<br />
Author (s) : Maneechotesuwan, K., Ekjiratrakul, W., Kasetsinsombat, K., Wongkajornsilp, A., Barnes, P.J.<br />
Title : Statins enhance the anti-inflammatory effects <strong>of</strong> inhaled corticosteroids in asthmatic patients through increased induction <strong>of</strong><br />
indoleamine 2, 3-dioxygenase<br />
Source : Journal <strong>of</strong> Allergy and Clinical Immunology, Volume 126, Issue 4, October <strong>2010</strong>, Pages 754-762.e1<br />
Document Type : Article<br />
Keywords : asthma; Indoleamine 2, 3-dioxygenase; inhaled corticosteroid; kynurenine; simvastatin<br />
274
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : Background: We have previously shown that inhaled corticosteroids activate indoleamine 2, 3-dioxygenase (IDO) activity<br />
through increased IL-10 secretion. Statins might enhance the anti-inflammatory effects <strong>of</strong> corticosteroids. Objective: In a<br />
double-blind study we added simvastatin to patients with mild asthma receiving a low dose <strong>of</strong> inhaled budesonide and<br />
evaluated sputum eosinophil counts, IL-10 secretion, and IDO activity, as well as their putative signaling pathways. Methods:<br />
After a 2-week run-in period without treatment, 50 asthmatic patients were treated with 200 μg <strong>of</strong> budesonide and randomly<br />
assigned to either 10 mg <strong>of</strong> simvastatin or matched placebo for 8 weeks. Inflammation was evaluated through eosinophil<br />
counts, secretory signaling molecules, and immunocytochemistry <strong>of</strong> macrophages in sputum. Results: Sputum eosinophil<br />
percentages were reduced significantly by the combined therapy with budesonide and simvastatin compared with<br />
budesonide alone (P = .02). Corticosteroids activated glucocorticoid-induced TNF receptor ligand, which induces activation <strong>of</strong><br />
p52 through the noncanonical nuclear factor κB pathway, leading to the increased transcription and activation <strong>of</strong> IDO.<br />
Simvastatin enhanced corticosteroid-activated noncanonical nuclear factor κB-dependent induction <strong>of</strong> IDO by activating type<br />
I interferons and also enhanced the effect <strong>of</strong> corticosteroid on IL-10 release. Conclusion: A statin enhances the antiinflammatory<br />
effect <strong>of</strong> an inhaled corticosteroid in asthma, and this was mediated through the alteration <strong>of</strong> IDO activity in<br />
macrophages. © <strong>2010</strong> American Academy <strong>of</strong> Allergy, Asthma & Immunology.<br />
No.752<br />
Author (s) : Maneechotesuwan, K., Sujaritwongsanon, P., Suthamsmai, T.<br />
Title : IgE production in allergic asthmatic patients with different asthma control status.<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand = Chotmaihet thangphaet, Volume 93 Suppl 1, January <strong>2010</strong>, Pages S71-78<br />
Document Type : Article<br />
Keywords : diagnostic agent, immunoglobulin E, methacholine chloride;<br />
adult, aged, article, asthma, blood, eosinophil, female, human, immunology, inhalation test, male, metabolism, middle<br />
aged, pathophysiology, skin test, spirometry; Adult, Aged, Asthma, Bronchial Provocation<br />
Tests, Eosinophils, Female, Humans, Immunoglobulin E, Male, Methacholine Chloride, Middle Aged, Skin Tests, Spirometry<br />
<strong>Abstract</strong> : BACKGROUND: Although much is known about the fact that IgE-mediated allergic inflammatory response contributes to airway<br />
inflammation, bronchial hyperresponsiveness, and asthma severity, little is known about the degree <strong>of</strong> IgE response in allergic<br />
asthmatics during treatment. OBJECTIVE: To determine the amount <strong>of</strong> total serum IgE among allergic asthmatic patients with<br />
various asthma controls. MATERIAL AND METHOD: A total <strong>of</strong> 190 non-smoking patients with allergic asthma were divided into<br />
three groups by using the asthma control definition according to the GINA 2006 criteria. There were 64 well-controlled, 88<br />
partly-controlled, and 38 uncontrolled. After study entry, patients underwent lung function test, methacholine challenge and<br />
skin prick test to establish allergic status. Peripheral venous blood specimens were collected to measure total IgE and<br />
absolute eosinophil numbers. The data are expressed as mean +/- SD. RESULTS: The logarithm <strong>of</strong> total serum IgE was<br />
significantly higher in subjects with uncontrolled allergic asthma than in those with well-controlled disease (p < .0001). IgE<br />
response in uncontrolled asthmatics was still high despite having been treated with ICS at a dose which was significantly high<br />
when compared with well-controlled subjects (1075.4 +/- 420 vs. 703.5 +/- 355, p < .0001). The logarithm <strong>of</strong> total serum IgE<br />
was associated with increased blood eosinophil counts (r = 0.25, p .0007) among three asthmatic groups and with decreased<br />
prebronchodilator FEV1 (r = -0.42, p = .0075) and PC20 (r = -0.36, p = .04) only in uncontrolled group. CONCLUSION: In allergic<br />
asthmatic patients with various disease control stages, there are differences in IgE immune response. Both high and nonsuppressible<br />
total serum IgE response may be involved in the development <strong>of</strong> uncontrolled asthma.<br />
No.753<br />
Author (s) : Maneechotesuwan, K., Wongkajornsilp, A., Huabprasert, S., Kasetsinsombat, K., Suthumansami, T.<br />
Title : Differential expression <strong>of</strong> Th2 chemokine receptors on T cells from atopic and nonatopic asthmatics in response to Der p 1pulsed<br />
dendritic cells.<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand = Chotmaihet thangphaet, Volume 93 Suppl 1, January <strong>2010</strong>, Pages S62-70<br />
Document Type : Article<br />
Keywords : allergen, CCL17 protein, human, CCL22 protein, human, chemokine receptor CCR3, chemokine receptor<br />
CCR4, Dermatophagoides pteronyssinus antigen p 1, house dust allergen, macrophage derived chemokine, messenger<br />
RNA, thymus and activation regulated chemokine; animal, article, asthma, CD4+ T lymphocyte, dendritic cell, flow<br />
cytometry, genetics, human, immunology, metabolism, Pyroglyphidae, reverse transcription polymerase chain reaction, Th2<br />
cell; Allergens, Animals, Antigens, Dermatophagoides, Asthma, CD4-Positive T-Lymphocytes, Chemokine CCL17, Chemokine<br />
275
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
CCL22, Dendritic Cells, Flow Cytometry, Humans, Pyroglyphidae, Receptors, CCR3, Receptors, CCR4, Reverse Transcriptase<br />
Polymerase Chain Reaction, RNA, Messenger, Th2 Cells<br />
<strong>Abstract</strong> : BACKGROUND: In vitro polarized human Th2 cells preferentially express the chemokine receptors CCR3 and CCR4 and migrate<br />
to their ligands: eotaxin, monocyte-derived chemokine (MDC) or CCL22, thymus- and activation-regulated chemokine (TARC)<br />
or CCL17. However little is known about the regulation <strong>of</strong> these chemokine receptor axes by Der p 1-pulsed dendritic cells in<br />
house dust mite (HDM)-sensitive and non-atopic asthmatics. OBJECTIVE: The aim was to investigate the modulatory effects <strong>of</strong><br />
Der p 1-pulsed DCs on the expression <strong>of</strong> CCR3 and CCR4 on CD4+ T cells <strong>of</strong> HDM-sensitive and non-atopic asthmatics.<br />
MATERIAL AND METHOD: Using real-time RT-PCR and flow cytometry analysis, the expression <strong>of</strong> CCR3 and CCR4 were assessed<br />
in autologous CD4+ T cells after co-incubation with Der p 1-pulsed DCs from these two asthmatic groups. We also<br />
determined the mRNA expression <strong>of</strong> CCR4 ligands TARC/CCL17 and MDC/CCL22 in monocyte-derived DCs after Der p 1<br />
pulsation. RESULTS: We performed flow cytometry analysis <strong>of</strong> CD4+ T cells from HDM-sensitive and non-atopic asthmatics,<br />
taken 24 and 48 h after co-incubation with Der p 1-pulsed DCs. We demonstrated that after co-incubation, there was a<br />
significant increase in CCR3+ and CCR4+ CD4+ T cells from HDM-sensitive asthmatics, which began to occur at 24 h and 48 h<br />
respectively, and corresponded to their expression at mRNA levels. In contrast, only CCR4 mRNA but not protein expression<br />
was increased in non-atopic CD4+ T cells. After Der p 1 pulsation, mRNA expression <strong>of</strong> CCR4-specific ligands (CCL17 and<br />
CCL22) was also markedly upregulated in HDM-sensitive DCs whereas only CCL17 gene expression was increased in nonatopic<br />
DCs. CONCLUSION: These data support the role <strong>of</strong> DCs in differential regulation <strong>of</strong> CCR3 and CCR4 on CD4+ T cells<br />
from HDM-sensitive and non-atopic asthmatics after Der p 1 exposure.<br />
No.754<br />
Author (s) : Maneerat, Y.; Khachansaksumeth, V.; Nintasen, R.; Chaisri, U.; Viriyavejakul, P.; Chartiburus, P.<br />
Title : HUMAN SAPHENOUS VEIN CULTURE IN CONDITIONS OF ENDOTHELIUM DYSFUNCTION AND HYPERLIPIDEMIA: AN EX VIVO<br />
MODEL FOR EARLY DEVELOPMENT OF ATHEROSCLEROSIS<br />
Source : ATHEROSCLEROSIS SUPPLEMENTS, Volume: 11, Issue: 2, Pages: 140-140, Meeting <strong>Abstract</strong>: MS151, Published: JUN 10 <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.755<br />
Author (s) : Manguin, S., Bangs, M.J., Pothikasikorn, J., Chareonviriyaphap, T.<br />
Title : Review on global co-transmission <strong>of</strong> human Plasmodium species and Wuchereria bancr<strong>of</strong>ti by Anopheles mosquitoes<br />
Source : Infection, Genetics and Evolution, Volume 10, Issue 2, March <strong>2010</strong>, Pages 159-177<br />
Document Type : Review<br />
Keywords : Africa; Americas; Anopheles; Asia; Australasia; Bancr<strong>of</strong>tian filariasis; Malaria; Plasmodium; Western Pacific<br />
<strong>Abstract</strong> : Malaria and lymphatic filariasis are two <strong>of</strong> the most common mosquito-borne parasitic diseases worldwide which can occur as<br />
concomitant human infections while also sharing common mosquito vectors. This review presents the most recent available<br />
information on the co-transmission <strong>of</strong> human Plasmodium species and Wuchereria bancr<strong>of</strong>ti by Anopheles mosquitoes.<br />
Important biological and epidemiological aspects are also described including the lifecycle <strong>of</strong> each parasite species and their<br />
specificities, the geographical biodiversity <strong>of</strong> each pathogen and their vectors where the parasites are co-endemic, and<br />
biological, environmental and climatic determinants influencing transmission. The co-transmission <strong>of</strong> each disease is<br />
illustrated from both a global perspective and a country level using Thailand as a study case. Different diagnostic methods<br />
are provided for the detection <strong>of</strong> the parasites in biological samples ranging from traditional to more recent molecular<br />
methods, including methodologies employing concomitant detection assays <strong>of</strong> W. bancr<strong>of</strong>ti and Plasmodium spp. parasites.<br />
The relevant issues <strong>of</strong> combined malaria and Bancr<strong>of</strong>tian filariasis control strategies are reviewed and discussed. © 2009<br />
Elsevier B.V. All rights reserved.<br />
No.756<br />
Author (s) : Manojlovic, N.T., Vasiljevic, P.J., Gritsanapan, W., Supabphol, R., Manojlovic, I.<br />
Title : Phytochemical and antioxidant studies <strong>of</strong> Laurera benguelensis growing in Thailand<br />
Source : Biological Research, Volume 43, Issue 2, <strong>2010</strong>, Pages 169-176<br />
Document Type : Article<br />
Keywords : Anthraquinones; Antioxidant activity; Laurera benguelensis; Xanthones<br />
276
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : The aim <strong>of</strong> this study was to investigate metabolites <strong>of</strong> the lichen Laurera benguelensis. A high-performance liquid<br />
chromatographic (HPLC) method has been developed for the characterization <strong>of</strong> xanthones and anthraquinones in extracts <strong>of</strong><br />
this lichen. Lichexanthone, secalonic acid D, norlichexanthon, parietin, emodin, teloschistin and citreorosein were detected in<br />
the lichen samples, which were collected from two places in Thailand. Components <strong>of</strong> the lichen were identified by relative<br />
retention time and spectral data. This is the first time that a detailed phytochemical analysis <strong>of</strong> the lichen L. benguelensis<br />
was reported and this paper has chemotaxonomic significance because very little has been published on the secondary<br />
metabolites present in Laurera species. Some <strong>of</strong> the metabolites were detected for the first time in the family<br />
Trypetheliaceae. The results <strong>of</strong> preliminary testing <strong>of</strong> benzene extract and its chlor<strong>of</strong>orm and methanol fractions showed that<br />
all samples showed a weak radical scavenging activity. The chlor<strong>of</strong>orm extract showed the highest antioxidant activity.<br />
No.757<br />
Author (s) : Manonai, J., Wattanayingcharoenchai, R., Sarit-Apirak, S., Vannatim, N., Chittacharoen, A.<br />
Title : Prevalence and risk factors <strong>of</strong> anorectal dysfunction in women with urinary incontinence<br />
Source : Archives <strong>of</strong> Gynecology and Obstetrics, Volume 281, Issue 6, June <strong>2010</strong>, Pages 1003-1007<br />
Document Type : Article<br />
Keywords : Anorectal dysfunction; Risk factor; Urinary incontinence<br />
<strong>Abstract</strong> : Purpose: To evaluate the prevalence and risk factors <strong>of</strong> anorectal dysfunction among women with urinary incontinence.<br />
Method: This cross-sectional study was conducted among women attending the urogynecology clinic. Women with symptoms<br />
<strong>of</strong> urinary incontinence were requested to complete a structured questionnaire. Results: Four hundred and thirteen women<br />
participated in the study and 43.8% had at least one anorectal symptom. The prevalence rates <strong>of</strong> anal incontinence,<br />
constipation, and difficult defecation were 8.0, 38.0, and 25.7%, respectively. The risk factors associated with anorectal<br />
dysfunction were age and number <strong>of</strong> parity. Conclusion: Anorectal dysfunction was prevalent among women with urinary<br />
incontinence; age and number <strong>of</strong> parity were the risk factors. All women with symptoms <strong>of</strong> urinary incontinence should be<br />
evaluated about anorectal symptoms. © 2009 Springer-Verlag.<br />
No.758<br />
Author (s) : Manonukul, J., Kajornvuthidej, S.<br />
Title : Sebaceous neoplasms in siriraj hospital, <strong>Mahidol</strong> university: A 9-year-retrospective study<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 8, August <strong>2010</strong>, Pages 978-991<br />
Document Type : Review<br />
Keywords : Retrospective study; Sebaceous neoplasms<br />
<strong>Abstract</strong> : Background: Sebaceous neoplasms are adnexal neoplasms that contain a varying number <strong>of</strong> sebocytes, i.e. large cells with<br />
lipid-laden vacuolated cytoplasm, soap-bubble in appearance, and crenate nuclei. They are uncommon compared to other<br />
adnexal neoplasms. Various sebaceous neoplasms with complex histopathologic features and varying degree <strong>of</strong> sebaceous<br />
cells differentiation have been described in the literature. Objectives: To study the prevalence <strong>of</strong> ebaceous neoplasms, i.e.<br />
nevus sebaceus, sebaceous hyperplasia, sebaceous adenoma, sebaceoma, sebaceous pithelioma, superficial epithelioma with<br />
sebaceous differentiation, and sebaceous carcinoma diagnosed in the Department <strong>of</strong> Pathology, Siriraj Hospital, <strong>Mahidol</strong><br />
<strong>University</strong> during the 9-year-period between 1997 and 2005. To study the prevalence <strong>of</strong> tumor transformation that occurs in<br />
nevus sebaceus. Material and Method: A retrospective study <strong>of</strong> all sebaceous neoplasms including Nevus sebaceous,<br />
sebaceous hyperplasia, sebaceous adenoma, sebaceoma, sebaceous epithelioma, superficial epithelioma with sebaceous<br />
differentiation, sebaceous carcinoma, and all neoplasms containing the term "sebaceous" was performed. All slides were reanalyzed<br />
and re-diagnosed, without knowledge <strong>of</strong> the previous diagnosis or any clinical data, according to the criteria<br />
described in the standard textbooks <strong>of</strong> dermatopathology by Elder, McKee. Comparison between the previous diagnoses and<br />
the reviewed diagnoses was performed to assess the initial accuracy <strong>of</strong> all sebaceous neoplasms diagnosed. Small-sized<br />
biopsies or biopsies that possess incomplete sebaceous differentiation, in which the sebocytes were few and subtle,<br />
sometimes are difficult to diagnose. In these instances, the clinical correlation was needed for positive diagnosis. Afterwards,<br />
these reviewed diagnoses were recorded and classified according to the patient's age, gender, and localization. Results: Two<br />
hundred seven sebaceous neoplasms (2.34%) from the 8819 skin biopsies that were taken in the Department <strong>of</strong> Pathology,<br />
Siriraj Hospital during the 9-year-period, were included. After exclusion <strong>of</strong> some authentically non-sebaceous neoplasms, 182<br />
sebaceous neoplasms were found. Nevus sebaceus (n = 85, 46.7%) and sebaceous hyperplasia (n = 64, 35.1%) were the two<br />
most common benign lesions. The others were sebaceoma (n = 3, 1.6%), sebaceous adenoma (n = 2, 1.1%), sebaceous<br />
epithelioma (n = 1. 0.5%), sebaceous carcinoma (n = 26, 14.3%), and one unclassified sebaceous lesion that could not be<br />
277
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
considered a neoplasm. Tumor degeneration was found in 14 nevus sebaceus in which 21 neoplasms existed, namely,<br />
trichilemmoma (wart)-like lesion (n = 4), primitive follicular induction (n = 7), syringocystadenoma papilliferum (n = 3),<br />
trichoblastoma (n = 3), and one <strong>of</strong> each <strong>of</strong> trichoepithelioma, sebaceous adenoma, tumor <strong>of</strong> follicular infundiculum, and<br />
mucoepidermoid carcinoma. Conclusion: Twenty-six sebaceous carcinomas out <strong>of</strong> 182 sebaceous neoplasms, occurring<br />
mostly on the patients' eyelids, were found. The most common sebaceous neoplasm was nevus sebaceus (n = 85); the<br />
prophylactic excision <strong>of</strong> this lesion was recommended as tumor degeneration was frequent (14 out <strong>of</strong> 85 cases). Epithelial<br />
membrane antigen (EMA) usually decorated both normal and abnormal sebocytes. It was very helpful in the detection <strong>of</strong><br />
sebocytes in basaloid cells in sebaceous neoplasms and among lymphoid cells within metastasized lymph nodes and a<br />
discriminant between sebaceous and onsebaceousneoplasms.<br />
No.759<br />
Author (s) : Manosuthi, W., Butler, D.M., Chantratita, W., Sukasem, C., Richman, D.D., Smith, D.M.<br />
Title : Patients infected with HIV type 1 subtype CRF01-AE and failing first-line nevirapine- and efavirenz-based regimens<br />
demonstrate considerable cross-resistance to etravirine<br />
Source : AIDS Research and Human Retroviruses, Volume 26, Issue 6, 1 June <strong>2010</strong>, Pages 609-611<br />
Document Type : Letter<br />
Keywords : didanosine, efavirenz, etravirine, lamivudine, lamivudine plus stavudine, nevirapine, stavudine, ten<strong>of</strong>ovir, virus<br />
RNA, zidovudine; clinical practice, codon, cross resistance, demography, drug efficacy, gene sequence, genetic<br />
analysis, genotype, highly active antiretroviral therapy, human, Human immunodeficiency virus 1, Human immunodeficiency<br />
virus infected patient, Human immunodeficiency virus infection, letter, priority journal, sequence analysis, Southeast<br />
Asia, Thailand, treatment failure, treatment outcome, viral genetics, virus gene, virus load, virus mutation, virus strain, world<br />
health organization; Anti-HIV Agents, Benzoxazines, Drug Resistance, Viral, HIV Infections, HIV-<br />
1, Humans, Nevirapine, Pyridazines, Treatment Failure<br />
<strong>Abstract</strong> : –<br />
No.760<br />
Author (s) : Manosuthi, W., Chetchotisakd, P., Nolen, T.L., Wallace, D., Sungkanuparph, S., Anekthananon, T., Supparatpinyo, K., Pappas,<br />
P.G., Larsen, R.A., Filler, S.G., Andes, D.<br />
Title : Monitoring and impact <strong>of</strong> fluconazole serum and cerebrospinal fluid concentration in HIV-associated cryptococcal meningitisinfected<br />
patients<br />
Source : HIV Medicine, Volume 11, Issue 4, April <strong>2010</strong>, Pages 276-281<br />
Document Type : Article<br />
Keywords : Antifungal; Cryptococcosis; High dose; Pharmacokinetic; Treatment<br />
<strong>Abstract</strong> : Objectives:The aim <strong>of</strong> the present study was to assess fluconazole pharmacokinetic measures in serum and cerebrospinal<br />
fluid (CSF); and the correlation <strong>of</strong> these measures with clinical outcomes <strong>of</strong> invasive fungal infections. Methods:A randomized<br />
trial was conducted in HIV-infected patients receiving three different regimens <strong>of</strong> fluconazole plus amphotericin B (AmB) for<br />
the treatment <strong>of</strong> cryptococcal meningitis. Regimens included fluconazole 400 mg/day+AmB (AmB+Fluc400) or fluconazole<br />
800 mg/day+AmB (AmB+Fluc800) (14 days followed by fluconazole alone at the randomized dose for 56 days); or AmB alone<br />
for 14 days followed by fluconazole 400 mg/day for 56 days. Serum (at 24 h after dosing) and CSF samples were taken at<br />
baseline and days 14 and 70 (serum only) for fluconazole measurement, using gas-liquid chromatography. Results: Sixty-four<br />
treated patients had fluconazole measurements: 11 in the AmB group, 12 in the AmB+Fluc400 group and 41 in the<br />
AmB+Fluc800 group. Day 14 serum concentration geometric means were 24.7 mg/L for AmB+Fluc400 and 37.0 mg/L for<br />
AmB+Fluc800. Correspondingly, CSF concentration geometric means were 25.1 mg/L and 32.7 mg/L. Day 14 Serum and CSF<br />
concentrations were highly correlated with AmB+Fluc800 (P
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.761<br />
Author (s) : Manosuthi, W., Mankatitham, W., Lueangniyomkul, A., Prasithsirikul, W., Tantanathip, P., Suntisuklappon, B., Narkksoksung, A.,<br />
Nilkamhang, S., Sungkanuparph, S.<br />
Title : Renal impairment after switching from stavudine/lamivudine to ten<strong>of</strong>ovir/lamivudine in NNRTI-based antiretroviral regimens<br />
Source : AIDS Research and Therapy, Volume 7, 11 October <strong>2010</strong>, Article number 37<br />
Document Type : Article<br />
Keywords : creatinine, efavirenz, lamivudine, nevirapine, phosphorus, stavudine, ten<strong>of</strong>ovir, ten<strong>of</strong>ovir disoproxil, uric acid;<br />
adult, article, clinical trial, creatinine blood level, drug substitution, female, glomerulus filtration rate, human, Human<br />
immunodeficiency virus infection, hypophosphatemia, kidney disease, major clinical study, male, phosphate blood<br />
level, priority journal, prospective study, proteinuria, side effect, uric acid blood level, urinalysis<br />
<strong>Abstract</strong> : Background: During stavudine phase-out plan in developing countries, ten<strong>of</strong>ovir is used to substitute stavudine. However,<br />
knowledge regarding whether there is any difference <strong>of</strong> the frequency <strong>of</strong> renal injury between ten<strong>of</strong>ovir/lamivudine/efavirenz<br />
and ten<strong>of</strong>ovir/lamivudine/nevirapine is lacking.Methods: This prospective study was conducted among HIV-infected patients<br />
who were switched NRTI from stavudine/lamivudine to ten<strong>of</strong>ovir/lamivudine in efavirenz-based (EFV group) and nevirapinebased<br />
regimen (NVP group) after two years <strong>of</strong> an ongoing randomized trial. All patients were assessed for serum phosphorus,<br />
uric acid, creatinine, estimated glomerular filtration rate (eGFR), and urinalysis at time <strong>of</strong> switching, 12 and 24 weeks.Results:<br />
Of 62 patients, 28 were in EFV group and 34 were in NVP group. Baseline characteristics and eGFR were not different between<br />
two groups. At 12 weeks, comparing mean ± SD measures between EFV group and NVP group were: phosphorus <strong>of</strong> 3.16 ±<br />
0.53 vs. 2.81 ± 0.42 mg/dL (P = 0.005), %patients with proteinuria were 15% vs. 38% (P = 0.050). At 24 weeks, mean ± SD<br />
phosphorus and median (IQR) eGFR between the corresponding groups were 3.26 ± 0.78 vs. 2.84 ± 0.47 mg/dL (P = 0.011) and<br />
110 (99-121) vs. 98 (83-112) mL/min (P = 0.008). In NVP group, comparing week 12 to time <strong>of</strong> switching, there was a<br />
decrement <strong>of</strong> phosphorus (P = 0.007) and eGFR (P = 0.034). By multivariate analysis, 'receiving nevirapine', 'old age' and 'low<br />
baseline serum phosphorus' were associated with hypophosphatemia at 24 weeks (P < 0.05). Receiving nevirapine and low<br />
baseline eGFR were associated with lower eGFR at 24 weeks (P < 0.05).Conclusion: The frequency <strong>of</strong> ten<strong>of</strong>ovir-associated<br />
renal impairment was higher in patients receiving ten<strong>of</strong>ovir/lamivudine/nevirapine compared to<br />
ten<strong>of</strong>ovir/lamivudine/efavirenz. Further studies regarding patho-physiology are warranted. © <strong>2010</strong> Manosuthi et al; licensee<br />
BioMed Central Ltd.<br />
No.762<br />
Author (s) : Manosuthi, W., Tantanathip, P., Chimsuntorn, S., Eampokarap, B., Thongyen, S., Nilkamhang, S., Sungkanuparph, S.<br />
Title : Treatment outcomes <strong>of</strong> patients co-infected with HIV and tuberculosis who received a nevirapine-based antiretroviral<br />
regimen: A four-year prospective study<br />
Source : <strong>International</strong> Journal <strong>of</strong> Infectious Diseases, Volume 14, Issue 11, November <strong>2010</strong>, Pages e1013-e1017<br />
Document Type : Article<br />
Keywords : HIV; Nevirapine; Rifampin; Thailand; Tuberculosis<br />
<strong>Abstract</strong> : Background: The concurrent use <strong>of</strong> nevirapine-based antiretroviral therapy (ART) and rifampin-containing anti-tuberculosis<br />
regimens for the treatment <strong>of</strong> HIV and tuberculosis (TB) is common in resource-limited countries. Long-term outcomes <strong>of</strong> this<br />
concurrent treatment are unknown. Methods: Seventy HIV-infected patients receiving rifampin for active TB (TB group) and 70<br />
HIV-mono-infected patients (control group) were enrolled to receive nevirapine 400. mg/day-based ART. All were followed<br />
through 4 years <strong>of</strong> ART. Plasma HIV-1 RNA and CD4 cell counts were monitored every 12 weeks until 96 weeks, and every 24<br />
weeks thereafter. Results: Of the 140 patients, the median (interquartile range (IQR)) CD4 count was 31 (14-79) cells/mm3 and<br />
median (IQR) plasma HIV-1 RNA was 5.6 (5.2-5.9) log copies/ml at baseline Thirty-nine (55.7%) patients in the TB group were<br />
diagnosed with extrapulmonary/disseminated TB. The median duration <strong>of</strong> concurrent administration <strong>of</strong> nevirapine and<br />
rifampin was 5.4 (4.6-6.1) months. By intention-to-treat analysis, the percentage <strong>of</strong> patients who achieved HIV-1 RNA <50<br />
copies/ml was 52.9% in the TB group and 50% in control group (p=0.866; odds ratio 1.121, 95% confidence interval 0.578-<br />
2.176); median (IQR) CD4 counts were 352 (271-580) cells/mm3 and 425 (308-615) cells/mm3 in the corresponding groups<br />
(p=0.238). The proportion <strong>of</strong> ART discontinuation due to any reason at 1, 2, 3, and 4 years was 21%, 34%, 37%, and 46% in<br />
the TB group and 21%, 36%, 43%, and 49% in the control group, respectively (p=0.651). The 4-year mortality rate was 6.4% in<br />
both groups. Conclusions: Nevirapine-based ART is an option for HIV-infected patients who receive rifampin in resourcelimited<br />
countries or those who cannot tolerate efavirenz. © <strong>2010</strong> <strong>International</strong> Society for Infectious Diseases.<br />
279
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.763<br />
Author (s) : Manuskiatti, W., Siriphukpong, S., Varothai, S., Wanitphakdeedecha, R., Fitzpatrick, R.E.<br />
Title : Effect <strong>of</strong> pulse width <strong>of</strong> a variable square pulse (VSP) erbium:YAG laser on the treatment outcome <strong>of</strong> periorbital wrinkles in<br />
Asians<br />
Source : <strong>International</strong> Journal <strong>of</strong> Dermatology, Volume 49, Issue 2, February <strong>2010</strong>, Pages 200-206<br />
Document Type : Article<br />
Keywords : adult, article, Asian, controlled study, cutaneous parameters, erbium YAG laser, esthetics, female, follow up, human, low<br />
level laser therapy, outcome assessment, rejuvenation, wrinkle; Adult, Esthetics, Female, Follow-Up Studies, Humans, Laser<br />
Therapy, Lasers, Solid-State, Middle Aged, Pain, Postoperative, Patient Satisfaction, Postoperative Care, Rejuvenation, Risk<br />
Assessment, Skin Aging, Thailand, Treatment Outcome<br />
<strong>Abstract</strong> : Background: Uses <strong>of</strong> appropriate laser parameters are essential for an optimum outcome with minimal risks <strong>of</strong> complications.<br />
Objective: To evaluate the effect <strong>of</strong> pulse width <strong>of</strong> a variable square pulse (VSP) Er:YAG laser on the treatment outcome.<br />
Methods: Twenty-four Thais with periorbital wrinkles received two treatments with a low-fluence, VSP Er:YAG laser resurfacing<br />
1 month apart. Subjects were randomly divided into two groups and treated with two different parameters including a pulse<br />
width <strong>of</strong> 0.3 ms (short pulse, SP) for one group and a pulse width <strong>of</strong> 250 ms (super-long pulse, SL) for the other group.<br />
Assessments were evaluated at baseline, 1-month and 3-month follow-up visits. Results: After one treatment, 63.7% and<br />
33.4% <strong>of</strong> the subjects in the SP and SL groups, respectively, were assessed to have obvious to marked improvement <strong>of</strong> their<br />
wrinkles. After two treatments, a higher percentage <strong>of</strong> the subjects were rated to have obvious to marked improvement<br />
including 91% and 66.7% in the SP and SL groups, respectively. There was no significant difference in therapeutic outcome<br />
between the SP and SL groups. Conclusions: There was no significant difference in efficacy and side effects, between the SP<br />
and SL groups. However, a trend toward better response with the shorter pulse width was observed. © <strong>2010</strong> The<br />
<strong>International</strong> Society <strong>of</strong> Dermatology.<br />
No.764<br />
Author (s) : Manuskiatti, W., Triwongwaranat, D., Varothai, S., Eimpunth, S., Wanitphakdeedecha, R.<br />
Title : Efficacy and safety <strong>of</strong> a carbon-dioxide ablative fractional resurfacing device for treatment <strong>of</strong> atrophic acne scars in Asians<br />
Source : Journal <strong>of</strong> the American Academy <strong>of</strong> Dermatology, Volume 63, Issue 2, <strong>2010</strong>, Pages 274-283<br />
Document Type : Article<br />
Keywords : ablative fractional resurfacing; Asians; atrophic acne scars; carbon dioxide; fractional photothermolysis<br />
<strong>Abstract</strong> : Background: Treatment <strong>of</strong> atrophic scars with a fractional laser resurfacing technique has demonstrated favorable outcomes,<br />
although data on the efficacy and adverse effects <strong>of</strong> this procedure in persons with dark-skinned phototypes are limited.<br />
Objective: This study was conducted to evaluate the efficacy and safety <strong>of</strong> carbon-dioxide ablative fractional resurfacing on<br />
atrophic acne scars in Asian individuals. Methods: Thirteen subjects (8 female and 5 male, aged 25-52 years) with skin<br />
phototype IV and atrophic acne scars were treated with 3 sessions <strong>of</strong> carbon-dioxide ablative fractional resurfacing laser on an<br />
average <strong>of</strong> 7-week interval. Objective (ultraviolet A-light video camera) and subjective (clinical evaluation by two blinded<br />
dermatologists) assessments were obtained at baseline and at 1, 3, and 6 months after the final treatment. Results: At the 6month<br />
follow-up, 85% <strong>of</strong> the subjects were rated as having at least 25% to 50% improvement <strong>of</strong> scars. Improvement<br />
significantly progressed from the 1-month follow-up to the 6-month follow-up (P = .002). At 1 month after 3 treatments,<br />
surface smoothness (P = .03) and scar volume (P < .001) significantly improved, compared with baseline measurements. Of<br />
the subjects, 62% rated themselves as having at least 50% improvement in their scars. Mild postinflammatory<br />
hyperpigmentation was the most common adverse effect observed in 92% <strong>of</strong> the subjects or 51% <strong>of</strong> treatment sessions, and<br />
was completely resolved in an average <strong>of</strong> 5 weeks. Limitation: The small sample size was a study limitation. Conclusions:<br />
Carbon-dioxide ablative fractional resurfacing appears to be effective and well tolerated for the treatment <strong>of</strong> atrophic acne<br />
scars in Asians. © 2009 by the American Academy <strong>of</strong> Dermatology, Inc.<br />
No.765<br />
Author (s) : Manuyakorn, W., Direkwattanachai, C., Benjaponpitak, S., Kamchaisatian, W., Sasisakulporn, C., Teawsomboonkit, W.<br />
Title : Sensitivity <strong>of</strong> Turbutester and Accuhaler tester in asthmatic children and adolescents<br />
Source : Pediatrics <strong>International</strong>, Volume 52, Issue 1, February <strong>2010</strong>, Pages 118-125<br />
Document Type : Article<br />
Keywords : Accuhaler-tester; Childhood asthma; Dry powder inhaler; Peak inspiratory flow rate; Turbutester<br />
280
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : Background: Dry powder inhalers (DPI) are alternative devices for delivering medication for treatment <strong>of</strong> asthma. The amount<br />
<strong>of</strong> drug delivery to the lungs is directly influenced by peak inspiratory flow rate (PIFR). A minimum PIFR <strong>of</strong>-30 L/min is needed<br />
for the Turbuhaler and Accuhaler. Methods: In order to evaluate the sensitivity <strong>of</strong> the Turbutester and Accuhaler tester in<br />
detecting the minimum and optimum PIFR for the Turbuhaler and Accuhaler in asthmatic children, PIFR was measured using<br />
the In-Check Dial through the internal resistance <strong>of</strong> the Turbuhaler and Accuhaler and compared according to the child's<br />
ability to make a whistle sound via both testers. Results: A total <strong>of</strong> 259 asthmatic children were studied: 20 pre-school<br />
children, aged 5-6 years; 174 school-age children, aged 7-12 years; and 65 adolescents, aged 13-18 years. The sensitivity <strong>of</strong><br />
the Turbutester and Accuhaler tester to detect optimum PIFR were 98.40% and 97.2%, respectively. In the comparison<br />
among age groups, the sensitivity <strong>of</strong> the Accuhaler tester to detect optimum or minimum PIFR for the Accuhaler was 95%,<br />
97.7% and 95.4%, respectively. The sensitivity <strong>of</strong> the Turbutester to detect optimum PIFR for the Turbuhaler was 94.4%,<br />
98.8% and 98.5%, respectively. The sensitivity <strong>of</strong> the Turbutester to detect minimum PIFR for the Turbuhaler was 94.7%,<br />
100% and 100%, respectively. There were no significant differences in percentage <strong>of</strong> having optimum or minimum PIFR<br />
among asthma severity and current device usage in all age groups. Conclusions: Most children aged at least 5 years could<br />
generate enough PIFR to use dry powder inhaler devices. Both the Turbutester and Accuhaler tester were found to have high<br />
sensitivity in detecting optimum and minimum required PIFR. © <strong>2010</strong> Japan Pediatric Society.<br />
No.766<br />
Author (s) : Manuyakorn, A., Paulus, R., Farrell, J., Dawson, N.A., Tze, S., Cheung-Lau, G., Hines, O.J., Reber, H., Seligson, D.B., Horvath, S.,<br />
Kurdistani, S.K., Guha, C., Dawson, D.W.<br />
Title : Cellular Histone Modification Patterns Predict Prognosis and Treatment Response in Resectable Pancreatic Adenocarcinoma:<br />
Results From RTOG 9704<br />
Source : JOURNAL OF CLINICAL ONCOLOGY, Volume: 28, Issue: 8, Pages: 1358-1365, DOI: 10.1200/JCO.2009.24.5639, Published: MAR 10<br />
<strong>2010</strong><br />
Document Type : Article<br />
Keywords : NUCLEOSIDE TRANSPORTER 1; CANCER-CELLS; THYMIDYLATE SYNTHASE; GEMCITABINE; H3; TRIMETHYLATION; 5-<br />
FLUOROURACIL; EXPRESSION; SURVIVAL; METHYLATION<br />
<strong>Abstract</strong> : Purpose Differences in cellular levels <strong>of</strong> histone modifications have predicted clinical outcome in certain cancers. Here, we<br />
studied the prognostic and predictive value <strong>of</strong> three histone modifications in pancreatic adenocarcinoma. Methods Tissue<br />
microarrays (TMAs) from two pancreatic adenocarcinoma cohorts were examined, including those from a 195-patient cohort<br />
from Radiation Therapy Oncology Group trial RTOG 9704, a multicenter, phase III, randomized treatment trial comparing<br />
adjuvant gemcitabine with fluorouracil and a 140-patient cohort <strong>of</strong> patients with stage I or II cancer from <strong>University</strong> <strong>of</strong><br />
California, Los Angeles Medical Center. Immunohistochemistry was performed for histone H3 lysine 4 dimethylation<br />
(H3K4me2), histone H3 lysine 9 dimethylation (H3K9me2), and histone H3 lysine 18 acetylation (H3K18ac). Positive tumor cell<br />
staining for each histone modification was used to classify patients into low-and high-staining groups, which were related to<br />
clinicopathologic parameters and clinical outcome measures. Results Low cellular levels <strong>of</strong> H3K4me2, H3K9me2, or H3K18ac<br />
were each significant and independent predictors <strong>of</strong> poor survival in univariate and multivariate models, and combined low<br />
levels <strong>of</strong> H3K4me2 and/or H3K18ac were the most significant predictor <strong>of</strong> overall survival (hazard ratio, 2.93; 95% Cl, 1.78 to<br />
4.82) in the <strong>University</strong> <strong>of</strong> California, Los Angeles cohort. In subgroup analyses, histone levels were predictive <strong>of</strong> survival<br />
specifically for those patients with node-negative cancer or for those patients receiving adjuvant fluorouracil, but not<br />
gemcitabine, in RTOG 9704. Conclusion Cellular levels <strong>of</strong> histone modifications define previously unrecognized subsets <strong>of</strong><br />
patients with pancreatic adenocarcinoma with distinct epigenetic phenotypes and clinical outcomes and represent prognostic<br />
and predictive biomarkers that could inform clinical decisions, including the use <strong>of</strong> fluorouracil chemotherapy.<br />
No.767<br />
Author (s) : Martella, S., Rietjens, M., Lohsiriwat, V., Lazzari, R., Vavassori, A., Jereczek, B.A., Lazzati, V., Leonardi, M.C., Petit, J.Y.<br />
Title : Acute radiation dermatitis in breast cancer: Topical therapy with vitamin E acetate in lipophilic gel base<br />
Source : ecancermedicalscience, Volume 4, Issue 1, 23 December <strong>2010</strong>, Article number 190<br />
Document Type : Article<br />
Keywords : Breast cancer; Radiation dermatitis; Vitamin E<br />
<strong>Abstract</strong> : Background: Radiotherapy can cause adverse skin reactions over the course <strong>of</strong> their treatment. Currently, management is<br />
based on several tropical products although there is no gold-standard approach to prevention and management <strong>of</strong> radiation<br />
toxicity. Method: We report our experience <strong>of</strong> vitamin E acetate in the treatment <strong>of</strong> radiation dermatitis in breast cancer<br />
281
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
patients who experienced grade 4 side effects (according to Radiation Therapy Oncology Group criteria). Results: Clinical<br />
management consisted <strong>of</strong> oral antibiotics and local application <strong>of</strong> vitamin E acetate and local escarectomy. All <strong>of</strong> the patients<br />
achieved complete re-epithelialization within 40 days. Conclusion: Skin ulceration and necrosis post-radiation may interrupt<br />
oncological treatment in breast cancer patients. In acute radiodermatitis with skin necrosis, we propose the use <strong>of</strong> oral<br />
antibiotics together with escarectomy and the application <strong>of</strong> vitamin E acetate to facilitate the healing process in order to<br />
minimize the interruption to the oncological treatment. © the authors; licensee ecancermedicalscience.<br />
No.768<br />
Author (s) : Martin, M., Vanichseni, S., Suntharasamai, P., Mock, P.A., van Griensven, F., Pitisuttithum, P., Tappero, J.W., Chiamwongpaet, S.,<br />
Sangkum, U., Kitayaporn, D., Gurwith, M., Choopanya, K.<br />
Title : Drug use and the risk <strong>of</strong> HIV infection amongst injection drug users participating in an HIV vaccine trial in Bangkok, 1999-2003<br />
Source : <strong>International</strong> Journal <strong>of</strong> Drug Policy, Volume 21, Issue 4, July <strong>2010</strong>, Pages 296-301<br />
Document Type : Article<br />
Keywords : Asia; Heroin; HIV infection; HIV vaccine; Methamphetamine<br />
<strong>Abstract</strong> : Background: HIV spread rapidly amongst injecting drug users (IDUs) in Bangkok in the late 1980s. In recent years, changes in<br />
the drugs injected by IDUs have been observed. We examined data from an HIV vaccine trial conducted amongst IDUs in<br />
Bangkok during 1999-2003 to describe drug injection practices, drugs injected, and determine if drug use choices altered the<br />
risk <strong>of</strong> incident HIV infection. Methods: The AIDSVAX B/E HIV vaccine trial was a randomized, double-blind, placebo-controlled<br />
trial. At enrolment and every 6 months thereafter, HIV status and risk behaviour were assessed. A proportional hazards model<br />
was used to evaluate demographic characteristics, incarceration, drug injection practices, sexual activity, and drugs injected<br />
during follow-up as independent predictors <strong>of</strong> HIV infection. Results: The proportion <strong>of</strong> participants injecting drugs, sharing<br />
needles, and injecting daily declined from baseline to month 36. Amongst participants who injected, the proportion injecting<br />
heroin declined (98.6-91.9%), whilst the proportions injecting methamphetamine (16.2-19.6%) and midazolam (9.9-31.9%)<br />
increased. HIV incidence was highest amongst participants injecting methamphetamine, 7.1 (95% CI, 5.4-9.2) per 100 person<br />
years. Injecting heroin and injecting methamphetamine were independently associated with incident HIV infection.<br />
Conclusions: Amongst AIDSVAX B/E vaccine trial participants who injected drugs during follow-up, the proportion injecting<br />
heroin declined whilst the proportion injecting methamphetamine, midazolam, or combinations <strong>of</strong> these drugs increased.<br />
Controlling for heroin use and other risk factors, participants injecting methamphetamine were more likely to become HIVinfected<br />
than participants not injecting methamphetamine. Additional HIV prevention tools are urgently needed including<br />
tools that address methamphetamine use. © <strong>2010</strong>.<br />
No.769<br />
Author (s) : Masa-Ah, P., Tuntawiroon, M., Soongsathitanon, S.<br />
Title : A novel scheme for standardized uptake value (SUV) calculation in PET scans<br />
Source : <strong>International</strong> Journal <strong>of</strong> Mathematical Models and Methods in Applied Sciences, Volume 4, Issue 4, <strong>2010</strong>, Pages 291-299<br />
Document Type : Article<br />
Keywords : Positron emission tomography combined computed tomography (PET/CT); Standardized uptake value (SUV); The digital<br />
imaging and communications in medicine (DICOM)<br />
<strong>Abstract</strong> : This paper presents a novel scheme for Standardized Uptake Value (SUV) calculation in PET scans. The results are compared<br />
with the SUV taken from the well-known application s<strong>of</strong>tware from GE healthcare. The performance <strong>of</strong> the scheme is<br />
evaluated by using the DICOM files taken from the widely used standard PET phantom scan. The comparison was performed<br />
with 2 different ROI sizes in 45 image slices and the Pearson's correlation is used. The results show that both systems are<br />
correlated significantly with 95% confidence. So the results ensure that his novel scheme for Standardized Uptake Value<br />
(SUV) calculation provides the correct SUV value. The strength <strong>of</strong> this scheme is that the interchangeability <strong>of</strong> the DICOM files<br />
can be done conveniently without the special application s<strong>of</strong>tware from any vendors.<br />
No.770<br />
Author (s) : Mast, T.C., Kierstead, L., Gupta, S.B., Nikas, A.A., Kallas, E.G., Novitsky, V., Mbewe, B., Pitisuttithum, P., Schechter, M., Vardas, E.,<br />
Wolfe, N.D., Aste-Amezaga, M., Casimiro, D.R., Coplan, P., Straus, W.L., Shiver, J.W.<br />
Title : <strong>International</strong> epidemiology <strong>of</strong> human pre-existing adenovirus (Ad) type-5, type-6, type-26 and type-36 neutralizing antibodies:<br />
Correlates <strong>of</strong> high Ad5 titers and implications for potential HIV vaccine trials<br />
Source : Vaccine, Volume 28, Issue 4, 22 January <strong>2010</strong>, Pages 950-957<br />
282
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Document Type : Article<br />
Keywords : Adenovirus; HIV; Vaccine; Vector<br />
<strong>Abstract</strong> : Replication-defective adenoviruses have been utilized as candidate HIV vaccine vectors. Few studies have described the<br />
international epidemiology <strong>of</strong> pre-existing immunity to adenoviruses. We enrolled 1904 participants in a cross-sectional<br />
serological survey at seven sites in Africa, Brazil, and Thailand to assess neutralizing antibodies (NA) for adenovirus types Ad5,<br />
Ad6, Ad26 and Ad36. Clinical trial samples were used to assess NA titers from the US and Europe. The proportions <strong>of</strong><br />
participants that were negative were 14.8% (Ad5), 31.5% (Ad6); 41.2% (Ad26) and 53.6% (Ad36). Adenovirus NA titers varied by<br />
geographic location and were higher in non-US and non-European settings, especially Thailand. In multivariate logistic<br />
regression analysis, geographic setting (non-US and non-European settings) was statistically significantly associated with having<br />
higher Ad5 titers; participants from Thailand had the highest odds <strong>of</strong> having high Ad5 titers (adjusted OR = 3.53, 95% CI: 2.24,<br />
5.57). Regardless <strong>of</strong> location, titers <strong>of</strong> Ad5NA were the highest and Ad36 NA were the lowest. Coincident Ad5/6 titers were<br />
lower than either Ad5 or Ad6 titers alone. Understanding pre-existing immunity to candidate vaccine vectors may contribute<br />
to the evaluation <strong>of</strong> vaccines in international populations. © 2009.<br />
No.771<br />
Author (s) : Matan, K., Ibuka, S., Morinaga, R., Chi, S., Lynn, J.W., Christianson, A.D., Lumsden, M.D., Sato, T.J.<br />
Title : Doping dependence <strong>of</strong> spin dynamics in electron-doped Ba( Fe 1-xCox)2As2<br />
Source : Physical Review B - Condensed Matter and Materials Physics, Volume 82, Issue 5, 18 August <strong>2010</strong>, Article number 054515<br />
Document Type : Article<br />
Keywords : –<br />
<strong>Abstract</strong> : The spin dynamics in single crystal, electron-doped Ba( Fe 1-xCox)2As2 has been investigated by inelastic neutron scattering<br />
over the full range from undoped to the overdoped regime. We observe damped magnetic fluctuations in the normal state<br />
<strong>of</strong> the optimally doped compound (x=0.06) that share a remarkable similarity with those in the paramagnetic state <strong>of</strong> the<br />
parent compound (x=0). In the overdoped superconducting compound (x=0.14), magnetic excitations show a gaplike<br />
behavior, possibly related to a topological change in the hole Fermi surface (Lifshitz transition) while the imaginary part <strong>of</strong> the<br />
spin susceptibility χ″ prominently resembles that <strong>of</strong> the overdoped cuprates. For the heavily overdoped,<br />
nonsuperconducting compound (x=0.24) the magnetic scattering disappears, which could be attributed to the absence <strong>of</strong> a<br />
hole Fermi-surface pocket observed by photoemission. © <strong>2010</strong> The American Physical Society.<br />
No.772<br />
Author (s) : Matan, K., Ono, T., Fukumoto, Y., Sato, T.J., Yamaura, J., Yano, M., Morita, K., Tanaka, H.<br />
Title : Pinwheel valence-bond solid and triplet excitations in the two-dimensional deformed kagome lattice<br />
Source : Nature Physics, Volume 6, Issue 11, November <strong>2010</strong>, Pages 865-869<br />
Document Type : Article<br />
Keywords : –<br />
<strong>Abstract</strong> : Determining ground states <strong>of</strong> correlated electron systems is fundamental to understanding unusual phenomena in<br />
condensed-matter physics. A difficulty, however, arises in a geometrically frustrated system in which the incompatibility<br />
between the global topology <strong>of</strong> an underlying lattice and local spin interactions gives rise to macroscopically degenerate<br />
ground states 1, potentially prompting the emergence <strong>of</strong> quantum spin states, such as resonating valence bond2-7 and<br />
valence-bond solid8-11 (VBS). Although theoretically proposed to exist in a kagome lattice-one <strong>of</strong> the most highly frustrated<br />
lattices in two dimensions being comprised <strong>of</strong> corner-sharing triangles-such quantum-fluctuation-induced states have not<br />
been observed experimentally. Here we report the first realization <strong>of</strong> the 'pinwheel' VBS ground state in the S=1/2 deformed<br />
kagome lattice antiferromagnet Rb 2 Cu 3 SnF 12 (refs 12, 13). In this system, a lattice distortion breaks the translational<br />
symmetry <strong>of</strong> the ideal kagome lattice and stabilizes the VBS state. © <strong>2010</strong> Macmillan Publishers Limited. All rights reserved.<br />
No.773<br />
Author (s) : Matangkasombut, O., Wattanawaraporn, R., Tsuruda, K., Ohara, M., Sugai, M., Mongkolsuk, S.<br />
Title : Cytolethal distending toxin from Aggregatibacter actinomycetemcomitans induces DNA damage, S/G2 cell cycle arrest, and<br />
caspase-independent death in a Saccharomyces cerevisiae model<br />
Source : Infection and Immunity, Volume 78, Issue 2, February <strong>2010</strong>, Pages 783-792<br />
Document Type : Article<br />
283
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : bacterial toxin, caspase, cytolethal distending toxin b, histone H2B, unclassified drug; Aggregatibacter<br />
actinomycetemcomitans, apoptosis, article, cell cycle arrest, cell cycle G2 phase, cell cycle S phase, cell death, DNA<br />
damage, Gram negative bacterium, homologous recombination, nonhuman, periodontitis, priority journal, protein<br />
expression, protein function, protein phosphorylation, Saccharomyces cerevisiae; Bacterial Toxins, Blotting,<br />
Western, Caspases, Cell Cycle, DNA Damage, Flow Cytometry, Gram-Negative Bacteria, Saccharomyces cerevisiae<br />
<strong>Abstract</strong> : Cytolethal distending toxin (CDT) is a bacterial toxin that induces G 2/M cell cycle arrest, cell distension, and/or apoptosis in<br />
mammalian cells. It is produced by several Gram-negative species and may contribute to their pathogenicity. The catalytic<br />
subunit CdtB has homology with DNase I and may act as a genotoxin. However, the mechanism by which CdtB leads to cell<br />
death is not yet clearly understood. Here, we used Saccharomyces cerevisiae as a model to study the molecular pathways<br />
involved in the function <strong>of</strong> CdtB from Aggregatibacter actinomycetemcomitans, a cause <strong>of</strong> aggressive periodontitis. We show<br />
that A. actinomycetemcomitans CdtB (AaCdtB) expression induces S/G 2 arrest and death in a DNase-catalytic residue and<br />
nuclear localization-dependent manner in haploid yeasts. Yeast strains defective in homologous recombination (HR) repair,<br />
but not other DNA repair pathways, are hypersensitive to AaCdtB, suggesting that HR is required for survival upon CdtB<br />
expression. In addition, yeast does not harbor the substrate for the other activity proposed for CdtB function, which is<br />
phosphatidylinositol-3,4,5- triphosphate phosphatase. Thus, these results suggest that direct DNA-damaging activity alone is<br />
sufficient for CdtB toxicity. To investigate how CdtB induces cell death, we examined the effect <strong>of</strong> CdtB in yeast strains with<br />
mutations in apoptotic regulators. Our results suggest that yeast death occurs independently <strong>of</strong> the yeast metacaspase gene<br />
YCA1 and the apoptosis-inducing factor AIF1 but is partially dependent on histone H2B serine 10 phosphorylation. Therefore,<br />
we report here the evidence that AaCdtB causes DNA damage that leads to nonapoptotic death in yeast and the first<br />
mutation that confers resistance to CdtB. Copyright © <strong>2010</strong>, American Society for Microbiology. All Rights Reserved.<br />
No.774<br />
Author (s) : Maude, R.J., Hassan, M.U., Ghose, A., Douthwaite, S.T., Abul Faiz, M., Dondorp, A.M.<br />
Title : Studies on severe Malaria are still possible and essential<br />
Source : Clinical Infectious Diseases, Volume 50, Issue 2, 15 January <strong>2010</strong>, Pages 281-282<br />
Document Type : Letter<br />
Keywords : artesunate, quinine; Africa, Cambodia, clinical research, clinical trial, cost effectiveness analysis, developing country, disease<br />
control, disease transmission, endemic disease, human, incidence, intensive care<br />
unit, letter, malaria, morbidity, mortality, multicenter study, population, priority journal, Thailand, Viet Nam;<br />
Antimalarials, Biomedical Research, Humans, Malaria, Severity <strong>of</strong> Illness Index<br />
<strong>Abstract</strong> : –<br />
No.775<br />
Author (s) : Maude, R.J., Lubell, Y., Socheat, D., Yeung, S., Saralamba, S., Pongtavornpinyo, W., Cooper, B.S., Dondorp, A.M., White, N.J.,<br />
White, L.J.<br />
Title : The role <strong>of</strong> mathematical modelling in guiding the science and economics <strong>of</strong> malaria elimination<br />
Source : <strong>International</strong> Health, Volume 2, Issue 4, December <strong>2010</strong>, Pages 239-246<br />
Document Type : Review<br />
Keywords : Control; Economic; Malaria; Mathematical modelling; Model; Policy<br />
<strong>Abstract</strong> : Unprecedented efforts are now underway to eliminate malaria from many regions. Despite the enormous financial resources<br />
committed, if malaria elimination is perceived as failing it is likely that this funding will not be sustained. It is imperative that<br />
methods are developed to use the limited data available to design site-specific, cost-effective elimination programmes.<br />
Mathematical modelling is a way <strong>of</strong> including mechanistic understanding to use available data to make predictions. Different<br />
strategies can be evaluated much more rapidly than is possible through trial and error in the field. Mathematical modelling<br />
has great potential as a tool to guide and inform current elimination efforts. Economic modelling weighs costs against<br />
characterised effects or predicted benefits in order to determine the most cost-efficient strategy but has traditionally used<br />
static models <strong>of</strong> disease not suitable for elimination. Dynamic mathematical modelling and economic modelling techniques<br />
need to be combined to contribute most effectively to ongoing policy discussions. We review the role <strong>of</strong> modelling in<br />
previous malaria control efforts as well as the unique nature <strong>of</strong> elimination and the consequent need for its explicit<br />
modelling, and emphasise the importance <strong>of</strong> good disease surveillance. The difficulties and complexities <strong>of</strong> economic<br />
evaluation <strong>of</strong> malaria control, particularly the end stages <strong>of</strong> elimination, are discussed. © <strong>2010</strong> Royal Society <strong>of</strong> Tropical<br />
Medicine and Hygiene.<br />
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No.776<br />
Author (s) : Maude, R.J., Woodrow, C.J., White, L.J.<br />
Title : Artemisinin antimalarials: Preserving the "magic bullet"<br />
Source : Drug Development Research, Volume 71, Issue 1, February <strong>2010</strong>, Pages 12-19<br />
Document Type : Article<br />
Keywords : Antimalarial; Artemisinin; Malaria; Resistance<br />
<strong>Abstract</strong> : The artemisinins are the most effective antimalarial drugs known. They possess a remarkably wide therapeutic index. These<br />
agents have been used in traditional Chinese herbal medicine for more than 2,000 years but were not subjected to scientific<br />
scrutiny until the 1970s. The first formal clinical trials <strong>of</strong> the artemisinins, and the development <strong>of</strong> methods for their industrial<br />
scale production, followed rapidly. A decade later, Chinese scientists shared their findings with the rest <strong>of</strong> the world; since<br />
then, a significant body <strong>of</strong> international trial evidence has confirmed these drugs to be far superior to any available<br />
alternatives. In particular, they have the ability to rapidly kill a broad range <strong>of</strong> asexual parasite stages at safe concentrations<br />
that are consistently achievable via standard dosing regimens. As their half-life is very short, there was also thought to be a<br />
low risk <strong>of</strong> resistance. These discoveries coincided with the appearance and spread <strong>of</strong> resistance to all the other major classes<br />
<strong>of</strong> antimalarials. As a result, the artemisinins now form an essential element <strong>of</strong> recommended first-line antimalarial treatment<br />
regimens worldwide. To minimize the risk <strong>of</strong> artemisinin resistance, they are recommended to be used to treat<br />
uncomplicated malaria in combination with other antimalarials as artemisinin combination therapies (ACTs). Their rollout has<br />
resulted in documented reductions in malaria prevalence in a number <strong>of</strong> African and Asian countries. Unfortunately, there are<br />
already worrisome early signs <strong>of</strong> artemisinin resistance appearing in western Cambodia. If this resistance were to spread, it<br />
would be disastrous for malaria control efforts worldwide. The enormous challenge for the international community is how to<br />
avert this catastrophe and preserve the effectiveness <strong>of</strong> this antimalarial "magic bullet". © 2009 Wiley-Liss, Inc.<br />
No.777<br />
Author (s) : Mayurasakorn, K., Sitphahul, P., Hongto, P.<br />
Title : Supplement <strong>of</strong> three eggs a week improves protein malnutrition in thai children from rural areas<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 3, March <strong>2010</strong>, Pages 301-309<br />
Document Type : Article<br />
Keywords : Egg supplements; Lipid pr<strong>of</strong>iles; Pre albumin; Protein malnutrition; Rural areas; Thai children<br />
<strong>Abstract</strong> : Background: Protein Malnutrition is one <strong>of</strong> the most important health indexes that affect children's growth and development.<br />
In Thailand National Health Survey 2004, 21.5% <strong>of</strong> primary school students living in the rural area were below the 90th<br />
percentile <strong>of</strong> the standard weight for age. Objective: To compare nutritional status with serum proteins and lipids, and to<br />
determine the effect <strong>of</strong> egg supplement for primary school students aged 6-15 years. Material and Method: A randomized<br />
experimental study was performed in 417 participants received an addition <strong>of</strong> either three or ten eggs per week for 12<br />
consecutive weeks to basal diet. Results: The anthropometric and biochemical indexes were measured, 29.1% and 20.8% <strong>of</strong><br />
whom were Protein Malnutrition according to serum albumin and PreAlbumin's criteria, respectively. Albumin and PreAlbumin<br />
levels were positively correlated with Total Cholesterol and LDL-C levels. No difference in any biochemical index has been<br />
found between 3 eggs/wk group and 10 eggs/wk group. Besides, due to continuing egg supplement, Total Cholesterol, LDL<br />
and ratio <strong>of</strong> Total Cholesterol to HDL level have decreased (p < 0.001) but albumin, PreAlbumin and HDL levels have<br />
demonstrated significantly increasing levels (p < 0.001). Conclusion: Nowadays in rural areas <strong>of</strong> Thailand there are still<br />
primary school students with protein malnutrition especially in rural area. An addition <strong>of</strong> at least 3 eggs/wk supplements can<br />
effectively correct the problem <strong>of</strong> protein malnutrition among primary school students at risk as shown by biochemical<br />
indices, and it benefits the blood cholesterol level as well.<br />
No.778<br />
Author (s) : Mayxay, M., Keomany, S., Khanthavong, M., Souvannasing, P., Stepniewska, K., Khomthilath, T., Keola, S., Pongvongsa, T.,<br />
Phompida, S., Ubben, D., Valecha, N., White, N.J., Newton, P.N.<br />
Title : A phase III, randomized, non-inferiority trial to assess the efficacy and safety <strong>of</strong> dihydroartemisinin-piperaquine in comparison<br />
with artesunate- mefloquine in patients with uncomplicated Plasmodium falciparum Malaria in Southern Laos<br />
Source : American Journal <strong>of</strong> Tropical Medicine and Hygiene, Volume 83, Issue 6, December <strong>2010</strong>, Pages 1221-1229<br />
Document Type : Article<br />
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Keywords : artesunate, artesunate lactab, artesunate plus mefloquine, dihydroartemisinin plus<br />
piperaquine, doxycycline, mefloquine, unclassified drug; abdominal<br />
pain, adolescent, adult, anemia, anorexia, article, cardiovascular symptom, chickenpox, child, clinical<br />
trial, coma, convulsion, coughing, dehydration, diarrhea, dizziness, drug efficacy, drug fever, drug induced headache, drug<br />
safety, drug treatment failure, epistaxis, female, fever, heart ventricle extrasystole, herpes<br />
labialis, human, hypertension, impetigo, influenza, insomnia, leukocyte count, lung, lymph node, major clinical study, malaria<br />
falciparum, male, multicenter study, mumps, nausea, nephrolithiasis, parasite clearance, parasitemia, phase 3 clinical<br />
trial, Plasmodium falciparum, preschool child, pruritus, QT prolongation, randomized controlled trial, reinfection, risk<br />
factor, school child, side effect, single drug dose, sinus arrhythmia, sinus bradycardia, sinus tachycardia, sore<br />
throat, splenomegaly, ST segment, survival, T wave, tinnitus, tonsillitis, upper respiratory tract<br />
infection, vertigo, vomiting, weakness; Adolescent, Adult, Antimalarials, Artemisinins, Child, Child, Preschool, Drug Therapy,<br />
Combination, Female, Humans, Laos, Malaria, Falciparum, Male, Mefloquine, Quinolines, Young Adult;<br />
Artesunate, Plasmodium falciparum<br />
<strong>Abstract</strong> : We conducted an open, randomized clinical trial <strong>of</strong> oral dihydroartemisinin-piperaquine (DP) versus artesunate-mefloquine<br />
(AM) in 300 patients in Laos with uncomplicated Plasmodium falciparum malaria as part <strong>of</strong> a multicentre study in Asia.<br />
Survival analysis and adjustment for re-infection showed that the 63-day cure rates (95% confidence interval [CI]) were 100%<br />
for AM and 99.5% (96.4-99.8%) for DP. The 63-day cure rates per protocol were 99% (97 <strong>of</strong> 98) for AM and 99.5% (196 <strong>of</strong> 197)<br />
for DP (P = 0.55).The difference (AM minus DP) in cure rates (95% CI) was -0.5% (-5.1 to 2.0%), which is within the 5% noninferiority<br />
margin. The median fever and parasite clearance times were also similar for AM and DP. The proportion <strong>of</strong> patients<br />
with at least one recorded potential adverse event was significantly higher in the AM group (38 <strong>of</strong> 87,44%) than in the DP<br />
group (57 <strong>of</strong> 182, 31%) (relative risk = 0.6, 95% CI = 0.4-0.9; P = 0.04). Dihydroartemisinin-piperaquine is not inferior to AM in<br />
the treatment <strong>of</strong> uncomplicated P. falciparum malaria in Laos and is associated with fewer adverse effects. The results <strong>of</strong> this<br />
study were similar to those <strong>of</strong> the larger multicentre study. Copyright © <strong>2010</strong> by The American Society <strong>of</strong> Tropical Medicine<br />
and Hygiene.<br />
No.779<br />
Author (s) : Mcgready, R., Ashley, E.A., Wuthiekanun, V., Tan, S.O., Pimanpanarak, M., Viladpai-Nguen, S.J., Jesadapanpong, W., Blacksell,<br />
S.D., Peacock, S.J., Paris, D.H., Day, N.P., Singhasivanon, P., White, N.J., Nosten, F.<br />
Title : Arthropod borne disease: The leading cause <strong>of</strong> fever in pregnancy on the thai-burmese border<br />
Source : PLoS Neglected Tropical Diseases, Volume 4, Issue 11, November <strong>2010</strong>, Article number e888<br />
Document Type : Article<br />
Keywords : adolescent, adult, anorexia, arthralgia, arthropod disease, article, blood cell count, cholecystitis, chorioamnionitis, cohort<br />
analysis, convalescence, dengue, dizziness, dysuria, endometritis, fatality, female, fever, gastroenteritis, gestational<br />
age, headache, human, leptospirosis, low birth weight, major clinical study, malaria, mixed infection, myalgia, newborn<br />
death, prediction, pregnancy, pregnancy outcome, prenatal care, pyelonephritis, recurrent disease, respiratory tract<br />
infection, rickettsiosis, scrub typhus, spontaneous abortion, stillbirth, symptomatology, typhoid<br />
fever, typhus, animal, communicable disease, disease transmission, microbiology, Myanmar, parasite<br />
vector, parasitology, pregnancy complication, refugee, Thailand, virology; Adolescent, Adult, Animals, Arthropod<br />
Vectors, Cohort Studies, Communicable Diseases, Female, Fever, Humans, Myanmar, Pregnancy, Pregnancy Complications,<br />
Infectious, Pregnancy Outcome, Refugees, Thailand, Young Adult<br />
<strong>Abstract</strong> : Background: Fever in pregnancy is dangerous for both mother and foetus. In the 1980's malaria was the leading cause <strong>of</strong><br />
death in pregnant women in refugee camps on the Thai-Burmese border. Artemisinin combination therapy has significantly<br />
reduced the incidence <strong>of</strong> malaria in the population. The remaining causes <strong>of</strong> fever in pregnancy are not well documented.<br />
Methodology: Pregnant women attending antenatal care, where weekly screening for malaria is routine, were invited to have<br />
a comprehensive clinical and laboratory screen if they had fever. Women were admitted to hospital, treated and followed up<br />
weekly until delivery. A convalescent serum was collected on day 21. Delivery outcomes were recorded. Principal Findings:<br />
Febrile episodes (n = 438) occurred in 5.0% (409/8,117) <strong>of</strong> pregnant women attending antenatal clinics from 7-Jan-2004 to 17-<br />
May-2006. The main cause was malaria in 55.5% (227/409). A cohort <strong>of</strong> 203 (49.6% <strong>of</strong> 409) women had detailed fever<br />
investigations and follow up. Arthropod-borne (malaria, rickettsial infections, and dengue) and zoonotic disease (leptospirosis)<br />
accounted for nearly half <strong>of</strong> all febrile illnesses, 47.3% (96/203). Coinfection was observed in 3.9% (8/ 203) <strong>of</strong> women, mostly<br />
malaria and rickettsia. Pyelonephritis, 19.7% (40/203), was also a common cause <strong>of</strong> fever. Once malaria, pyelonephritis and<br />
acute respiratory illness are excluded by microscopy and/or clinical findings, one-third <strong>of</strong> the remaining febrile infections will<br />
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be caused by rickettsia or leptospirosis. Scrub and murine typhus were associated with poor pregnancy outcomes including<br />
stillbirth and low birth weight. One woman died (no positive laboratory tests). Conclusion/Significance: Malaria remains the<br />
leading cause <strong>of</strong> fever in pregnancy on the Thai-Burmese border. Scrub and murine typhus were also important causes <strong>of</strong><br />
fever associated with poor pregnancy outcomes. Febrile pregnant women on the Thai-Burmese border who do not have<br />
malaria, pyelonephritis or respiratory tract infection should be treated with azithromycin, effective for typhus and<br />
leptospirosis. © <strong>2010</strong> McGready et al.<br />
No.780<br />
Author (s) : McGready, R., Blacksell, S.D., Luksameetanasan, R., Wuthiekanun, V., Jedsadapanpong, W., Day, N.P.J., Nosten, F.<br />
Title : First report <strong>of</strong> an orientia tsutsugamushi type TA716-related scrub typhus infection in Thailand<br />
Source : Vector-Borne and Zoonotic Diseases, Volume 10, Issue 2, 1 March <strong>2010</strong>, Pages 191-193<br />
Document Type : Article<br />
Keywords : Orientia tsutsugamushi; Pregnancy; Scrub typhus; TA716<br />
<strong>Abstract</strong> : Orientia tsutsugamushi causes scrub typhus and is a rural zoonosis endemic in the Asia Pacific region. This is the first report <strong>of</strong><br />
O. tsutsugamushi TA716-like strain in a human in Thailand. The patient was in the 1st trimester <strong>of</strong> pregnancy when she<br />
developed scrub typhus. The O. tsutsugamushi strain TA716 was detected from her admission blood sample, and the<br />
pregnancy ended in spontaneous abortion. The effects <strong>of</strong> scrub typhus in pregnant women and the pregnancy outcome are<br />
sparsely documented in the published medical literature. Improved clinical recognition and laboratory diagnosis will be<br />
essential to better define the morbidity caused by this zoonosis especially in pregnancy. © <strong>2010</strong>, Mary Ann Liebert, Inc.<br />
No.781<br />
Author (s) : McGready, R., Nosten, F.<br />
Title : Which drug is effective and safe for acute malaria in pregnancy? Reviewing the evidence<br />
Source : Drug Development Research, Volume 71, Issue 1, February <strong>2010</strong>, Pages 56-68<br />
Document Type : Review<br />
Keywords : Malaria; P. falciparum; Pregnancy<br />
<strong>Abstract</strong> : During pregnancy, a woman living or travelling in a malaria endemic area is more at risk <strong>of</strong> contracting the disease and<br />
developing a severe infection and dying than a non-pregnant woman. Despite this increased morbidity and mortality in<br />
pregnancy, there are almost no studies on which to base recommendations on the use <strong>of</strong> antimalarial drugs in this<br />
vulnerable group. This is because, paradoxically, the emphasis is <strong>of</strong>ten put on the safety <strong>of</strong> the unborn child rather than that<br />
<strong>of</strong> the infested mother. As a result <strong>of</strong> this neglect, tens <strong>of</strong> thousands <strong>of</strong> pregnant women (and their fetuses) are dying every<br />
year <strong>of</strong> a very preventable and treatable infection. In recent years, some trials have been conducted, especially in areas <strong>of</strong><br />
high resistance in Plasmodium falciparum in South East Asia. The results show that quinine plus clindamycin is the treatment<br />
<strong>of</strong> choice in the first trimester, while artemisinin treatment should be used in the second and third trimesters in the<br />
treatment <strong>of</strong> uncomplicated malaria. For severe malaria, parenteral artesunate is the treatment <strong>of</strong> choice. However these<br />
studies have also shown that the pharmacokinetic properties <strong>of</strong> most antimalarials are altered during gestation and that the<br />
doses used in non-pregnant adults are <strong>of</strong>ten not adapted to pregnancy. Urgent efforts are required to optimize the treatment<br />
<strong>of</strong> malaria in pregnancy. © 2009 Wiley-Liss, Inc.<br />
No.782<br />
Author (s) : McGready, R., Wuthiekanun, V., Ashley, E.A., Tan, S.O., Pimanpanarak, M., Viladpai-nguen, S.J., Jesadapanpong, W., Blacksell,<br />
S.D., Proux, S., Day, N.P., Singhasivanon, P., White, N.J., Nosten, F., Peacock, S.J.<br />
Title : Diagnostic and treatment difficulties <strong>of</strong> pyelonephritis in pregnancy in resource-limited settings<br />
Source : American Journal <strong>of</strong> Tropical Medicine and Hygiene, Volume 83, Issue 6, December <strong>2010</strong>, Pages 1322-1329<br />
Document Type : Article<br />
Keywords : amoxicillin plus clavulanic acid, ampicillin, artesunate, ceftriaxone, cipr<strong>of</strong>loxacin, cotrimoxazole, extended spectrum beta<br />
lactamase, ketone, nitrite, paracetamol, protein; adult, antibiotic sensitivity, article, bacterial count, blood cell count, blood<br />
culture, Citrobacter, Enterococcus, epithelium cell, Escherichia coli, female, fever, human, Klebsiella, Klebsiella<br />
oxytoca, leukocyte, major clinical study, malaria falciparum, microscopy, pregnancy, pregnant woman, pyelonephritis, single<br />
drug dose, urinary tract infection, urine culture, urine sediment; Anti-Bacterial Agents, Bacteria, Drug Resistance,<br />
Bacterial, Female, Humans, Pregnancy, Pregnancy Complications, Infectious, Pyelonephritis, Risk Factors, Thailand; Escherichia<br />
coli<br />
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<strong>Abstract</strong> : Limited microbiology services impede adequate diagnosis and treatment <strong>of</strong> common infections such as pyelonephritis in<br />
resource-limited settings. Febrile pregnant women attending antenatal clinics at Shoklo Malaria Research Unit were <strong>of</strong>fered<br />
urine dipstick, sediment microscopy, urine culture, and a 5-mL blood culture. The incidence <strong>of</strong> pyelonephritis was 11/1,000<br />
deliveries (N = 53 in 4,819 pregnancies) between January 7,2004 and May 17,2006. Pyelonephritis accounted for 20.2%<br />
(41/203) <strong>of</strong> fever cases in pregnancy. Escherichia coli was the most commonly isolated pathogen: 87.5% (28/32) <strong>of</strong> organisms<br />
cultured. Susceptibility <strong>of</strong> E. coli to ampicillin (14%), cotrimoxazole (21%), and amoxicillin-clavulanic acid (48%) was very low.<br />
E. coli was susceptible to ceftriaxone and cipr<strong>of</strong>loxacin. The rate <strong>of</strong> extended spectrum β-lactamase (4.2%; 95% confidence<br />
interval = 0.7-19.5) was low. The rate and causes <strong>of</strong> pyelonephritis in pregnant refugee and migrant women were comparable<br />
with those described in developed countries. Diagnostic innovation in microbiology that permits affordable access is a high<br />
priority for resource-poor settings. Copyright © <strong>2010</strong> by The American Society <strong>of</strong> Tropical Medicine and Hygiene.<br />
No.783<br />
Author (s) : McKnight-Eily, L., Arrazola, R., Merritt, R., Malarcher, A., Sirichotiratana, N.<br />
Title : Prevalence and Psychosocial Correlates <strong>of</strong> Current Smoking Among Adolescent Students in Thailand, 2005<br />
Source : Health Education and Behavior, Volume 37, Issue 6, December <strong>2010</strong>, Pages 863-878<br />
Document Type : Article<br />
Keywords : adolescents; psychosocial factors; smoking; Thailand; tobacco<br />
<strong>Abstract</strong> : This article examines the prevalence <strong>of</strong> current smoking and associated psychosocial correlates and whether these correlates<br />
differ by sex among adolescent students in Thailand. Data were analyzed from the Thailand Global Youth Tobacco Survey<br />
(GYTS), a school-based, cross-sectional survey conducted in 2005 and completed by Mathayom 1, 2, and 3 (U.S. seventh<br />
through ninth grades) students. Weighted prevalence estimates <strong>of</strong> the percentage <strong>of</strong> students who were current smokers<br />
(smoked on ≥1 day during the past 30 days) and noncurrent smokers were calculated for the sample and for each<br />
psychosocial variable. Separate logistic regression models were calculated for males and females to examine the<br />
independent association <strong>of</strong> the psychosocial correlates <strong>of</strong> current smoking. Significant correlates for both males and females<br />
included close peer smoking, secondhand smoke exposure, being <strong>of</strong>fered a free cigarette by a tobacco industry<br />
representative, and belief that smoking is not harmful. These correlates are examined in the context <strong>of</strong> comprehensive<br />
tobacco control laws in Thailand. © <strong>2010</strong> by SOPHE.<br />
No.784<br />
Author (s) : Meankaew, P., Kaewkungwal, J., Khamsiriwatchara, A., Khunthong, P., Singhasivanon, P., Satimai, W.<br />
Title : Application <strong>of</strong> mobile-technology for disease and treatment monitoring <strong>of</strong> malaria in the "Better Border Healthcare<br />
Programme"<br />
Source : Malaria Journal, Volume 9, Issue 1, <strong>2010</strong>, Article number 237<br />
Document Type : Article<br />
Keywords : antimalarial agent; adolescent, adult, antimicrobial therapy, article, child, chill, controlled study, data base, diarrhea, disease<br />
surveillance, disease transmission, drug induced headache, fatigue, female, fever, follow up, geographic information<br />
system, health care, health care personnel, health care utilization, health program, human, immigrant, integrated health care<br />
system, major clinical study, malaria, malaria control, malaria falciparum, male, medical information system, medical<br />
practice, medical technology, minority group, mobile phone, multidrug resistance, myalgia, outpatient department, patient<br />
compliance, patient monitoring, Plasmodium falciparum, Plasmodium vivax, Plasmodium vivax malaria, preventive health<br />
service, pr<strong>of</strong>essional practice, school child, self report, symptom, Thailand, vomiting, world health<br />
organization, aged, infant, Internet, methodology, middle aged, newborn, preschool child, public health service, rural<br />
population, statistics, telemedicine; Adolescent, Adult, Aged, Aged, 80 and over, Cellular Phone, Child, Child,<br />
Preschool, Female, Humans, Infant, Infant, Newborn, Internet, Malaria, Falciparum, Malaria, Vivax, Male, Medication<br />
Adherence, Middle Aged, Public Health Administration, Rural Population, Telemedicine, Thailand, Young Adult<br />
<strong>Abstract</strong> : Background: The main objective <strong>of</strong> this study was to assess the effectiveness <strong>of</strong> integrating the use <strong>of</strong> cell-phones into a<br />
routine malaria prevention and control programme, to improve the management <strong>of</strong> malaria cases among an under-served<br />
population in a border area. The module for disease and treatment monitoring <strong>of</strong> malaria (DTMM) consisted <strong>of</strong> case<br />
investigation and case follow-up for treatment compliance and patients' symptoms. Methods: The module combining webbased<br />
and mobile technologies was developed as a pro<strong>of</strong> <strong>of</strong> concept, in an attempt to replace the existing manual, paperbased<br />
activities that malaria staff used in treating and caring for malaria patients in the villages for which they were<br />
responsible. After a patient was detected and registered onto the system, case-investigation and treatment details were<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
recorded into the malaria database. A follow-up schedule was generated, and the patient's status was updated when the<br />
malaria staff conducted their routine home visits, using mobile phones loaded with the follow-up application module. The<br />
module also generated text and graph messages for a summary <strong>of</strong> malaria cases and basic statistics, and automatically fed to<br />
predetermined malaria personnel for situation analysis. Following standard public-health practices, access to the patient<br />
database was strictly limited to authorized personnel in charge <strong>of</strong> patient case management. Results: The DTMM module was<br />
developed and implemented at the trial site in late November 2008, and was fully functioning in 2009. The system captured<br />
534 malaria patients in 2009. Compared to paper-based data in 2004-2008, the mobile-phone-based case follow-up rates by<br />
malaria staff improved significantly. The follow-up rates for both Thai and migrant patients were about 94-99% on Day 7<br />
(Plasmodium falciparum) and Day 14 (Plasmodium vivax) and maintained at 84-93% on Day 90. Adherence to anti-malarial<br />
drug therapy, based on self-reporting, showed high completion rate for P. falciparum-infected cases, but lower rate for P.<br />
vivax cases. Patients' symptoms were captured onto the mobile phone during each follow-up visit, either during the home<br />
visit or at Malaria Clinic; most patients had headache, muscle pain, and fatigue, and some had fever within the first follow-up<br />
day (day7/14) after the first anti-malarial drug dose. Conclusions: The module was successfully integrated and functioned as<br />
part <strong>of</strong> the malaria prevention and control programme. Despite the bias inherent in sensitizing malaria workers to perform<br />
active case follow-up using the mobile device, the study proved for its feasibility and the extent to which community<br />
healthcare personnel in the low resource settings could potentially utilize it efficiently to perform routine duties, even in<br />
remote areas. The DTMM has been modified and is currently functioning in seven provinces in a project supported by the<br />
WHO and the Bill & Melinda Gates Foundation, to contain multi-drug resistant malaria on the Thai-Cambodian border. © <strong>2010</strong><br />
Meankaew et al; licensee BioMed Central Ltd.<br />
No.785<br />
Author (s) : Medana, I.M., Day, N.P.J., Roberts, R., Sachanonta, N., Turley, H., Pongponratn, E., Hien, T.T., White, N.J., Turner, G.D.H.<br />
Title : Induction <strong>of</strong> the vascular endothelial growth factor pathway in the brain <strong>of</strong> adults with fatal falciparum malaria is a nonspecific<br />
response to severe disease<br />
Source : Histopathology, Volume 57, Issue 2, August <strong>2010</strong>, Pages 282-294<br />
Document Type : Article<br />
Keywords : cerebral; hypoxia; immunohistochemistry; malaria; pathophysiology; Plasmodium falciparum<br />
<strong>Abstract</strong> : Aims: Pathological or neuroprotective mechanisms in the brain in severe malaria may arise from microvascular obstruction<br />
with malaria-parasitized erythrocytes. This study aimed to investigate the role <strong>of</strong> hypoxia and induction <strong>of</strong> the vascular<br />
endothelial growth factor (VEGF) pathway in the neuropathophysiology <strong>of</strong> severe malaria. Methods and results:<br />
Immunohistochemistry was performed on post mortem brain tissue sections from 20 cases <strong>of</strong> severe malaria and examined<br />
for the expression <strong>of</strong> transcriptional regulators <strong>of</strong> VEGF [hypoxia-inducible factor-1 alpha (HIF-1), HIF-2], DEC-1, VEGF, VEGF<br />
receptors 1 and 2, and the activated, phosphorylated VEGF receptor 2 (pKDR). HIFs showed limited protein expression andor<br />
translocation to cell nuclei in severe malaria, but DEC-1, which is more stable and regulated by HIF-1, was observed. There<br />
was heterogeneous expression <strong>of</strong> VEGF and its receptors in severe malaria and non-malarial disease controls. pKDR expression<br />
on vessels was greater in malaria cases than in controls but did not correlate with parasite sequestration. VEGF uptake by<br />
malaria parasites was observed. Conclusions: VEGF and its receptor expression levels in severe malaria reflect a non-specific<br />
response to severe systemic disease. Potential manipulation <strong>of</strong> events at the vasculature by the parasite requires further<br />
investigation. © <strong>2010</strong> Blackwell Publishing Limited.<br />
No.786<br />
Author (s) : Meechonkit, P., Kovitvadhi, U., Chatchavalvanich, K., Sretarugsa, P., Weerachatyanukul, W.<br />
Title : Localization <strong>of</strong> serotonin in neuronal ganglia <strong>of</strong> the freshwater pearl mussel, Hyriopsis (Hyriopsis) bialata<br />
Source : Journal <strong>of</strong> Molluscan Studies, Volume 76, Issue 3, August <strong>2010</strong>, Pages 267-274<br />
Document Type : Article<br />
Keywords : bivalve, endocrine disruptor, larva, neurology, organic compound, physiological response, survival;<br />
Bivalvia, Hyriopsis, Pyrus, Unionidae<br />
<strong>Abstract</strong> : We investigated the histological features <strong>of</strong> neuronal ganglia and the localization <strong>of</strong> serotonin in the unionid mussel Hyriopsis<br />
(Hyriopsis) bialata in Thailand. The long-term goal <strong>of</strong> this study is to apply endocrine manipulation to enhance the<br />
synchronous release <strong>of</strong> larvae for in vitro culture <strong>of</strong> this freshwater mussel and to increase larval survival during settlement.<br />
Unlike the case in other bivalves, the cerebropleural ganglia in H. bialata were completely fused to form a single pear-shaped<br />
structure, and the pedal ganglia were situated within the visceral mass rather than in the foot. In all three ganglia <strong>of</strong> this<br />
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mussel, the neuronal cell bodies were clustered at the periphery <strong>of</strong> the ganglia, and the nerve fibres were bundled in the<br />
central region. Of the three types <strong>of</strong> cells present in the ganglia, the large neurons were the only ones to stain with<br />
paraldehyde fuchsin, indicating a neurosecretory function. These same neurons were also intensely reactive with antiserotonin,<br />
demonstrating their dual function. An intense staining <strong>of</strong> anti-serotonin was also observed in the nerve fibres and<br />
termini, particularly in the visceral ganglia. This suggests that serotonin may be conveyed from the large neurons within the<br />
visceral ganglion via an axonal route to reach the target reproductive organs, one <strong>of</strong> which is the demibranch, to stimulate<br />
larval release in this mussel. © The Author <strong>2010</strong>.<br />
No.787<br />
Author (s) : Meemon, K., Khawsuk, W., Sriburee, S., Meepool, A., Sethadavit, M., Sansri, V., Wanichanon, C., Sobhon, P.<br />
Title : Fasciola gigantica: Histology <strong>of</strong> the digestive tract and the expression <strong>of</strong> cathepsin L<br />
Source : Experimental Parasitology, Volume 125, Issue 4, August <strong>2010</strong>, Pages 371-379<br />
Document Type : Article<br />
Keywords : Cathepsin L; Digestive tract; Expression; Fasciola gigantica; Histology; Immun<strong>of</strong>luorescence; Immunogold; In situ<br />
hybridization; Trematode<br />
<strong>Abstract</strong> : The digestive tract <strong>of</strong> Fasciola gigantica is composed <strong>of</strong> the oral sucker, buccal tube, pharynx, esophagus, and caecum. The<br />
tegumental-type epithelium lines the first four parts <strong>of</strong> the digestive tract while the caecal-type epithelium lines the<br />
remaining parts from the caecal bifurcation. The caecal-epithelial cells are classified into 3 types according to their staining<br />
properties and ultrastructural characteristics, as related to the amount <strong>of</strong> food contents in the caecal lumen. All caecal-type<br />
epithelial cells synthesize and secrete cathepsin L, a major group <strong>of</strong> enzymes in the digestive tract, as detected by in situ<br />
hybridization and immunolocalization. Moreover, the secreted cathepsin L is also adsorbed on the outer surface <strong>of</strong> the<br />
tegument and the glycocalyx coating <strong>of</strong> the surface <strong>of</strong> the tegument, whereas the tegumental cells and tegumental<br />
syncytium covering the parasite's body and lining the proximal part <strong>of</strong> the digestive tract exhibit no in situ hybridization signal<br />
and immunostaining for cathepsin L. © <strong>2010</strong> Elsevier Inc.<br />
No.788<br />
Author (s) : Meksiri, S., Vichienchom, K., Wilairat, D.<br />
Title : A 1 GHz CMOS analog equalizer for perpendicular magnetic recording<br />
Source : IEEE Region 10 Annual <strong>International</strong> Conference, Proceedings/TENCON, <strong>2010</strong>, Article number 5686144, Pages 1521-1524<br />
Document Type : Conference Paper<br />
Keywords : Analog signal processing; CMOS analog circuit; Discrete-time equalizer; FIR; Perpendicular magnetic recording<br />
<strong>Abstract</strong> : This paper describes the design <strong>of</strong> a CMOS analog discrete-time equalizer for perpendicular magnetic recording (PMR) read<br />
channel. In this design the structure <strong>of</strong> analog FIR filter that places rotating switch matrix between DAC and multiplier has<br />
been proposed. It reduces an accumulative switching error in analog samples due to rotating switch matrix. A 7-tap filter<br />
circuit based on GPR2 target was designed and simulated using TSMC 0.18 μm CMOS process parameters. Simulation results<br />
show good agreement with the results <strong>of</strong> the system level simulation. At 1 GHz sampling frequency the equalizer dissipates<br />
1.5 mW. ©<strong>2010</strong> IEEE.<br />
No.789<br />
Author (s) : Menakongka, A., Suthiphongchai, T.<br />
Title : Involvement <strong>of</strong> PI3K and ERK1/2 pathways in hepatocyte growth factor-induced cholangiocarcinoma cell invasion<br />
Source : World Journal <strong>of</strong> Gastroenterology, Volume 16, Issue 6, 14 February <strong>2010</strong>, Pages 713-722<br />
Document Type : Article<br />
Keywords : Cholangiocarcinoma; Extracellular signal-regulated kinase; Hepatocyte growth factor; Invasion; Phosphoinositide 3-kinase<br />
<strong>Abstract</strong> : AIM: To investigate the role <strong>of</strong> hepatocyte growth factor (HGF) in cholangiocarcinoma (CCA) cell invasiveness and the<br />
mechanisms underlying such cellular responses. METHODS: Effects <strong>of</strong> HGF on cell invasion and motility were investigated in<br />
two human CCA cell lines, HuCCA-1 and KKU-M213, using Transwell in vitro assay. Levels <strong>of</strong> proteins <strong>of</strong> interest and their<br />
phosphorylated forms were determined by Western blotting. Localization <strong>of</strong> E-cadherin was analyzed by<br />
immun<strong>of</strong>luorescence staining and visualized under confocal microscope. Activities <strong>of</strong> matrix degrading enzymes were<br />
determined by zymography. RESULTS: Both CCA cell lines expressed higher Met levels than the H69 immortalized<br />
cholangiocyte cell line. HGF induced invasion and motility <strong>of</strong> the cell lines and altered E-cadherin from membrane to<br />
cytoplasm localization, but did not affect the levels <strong>of</strong> secreted matrix metalloproteinase (MMP)-2, MMP-9 and urokinase<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
plasminogen activator, key matrix degrading enzymes involved in cell invasion. Concomitantly, HGF stimulated Akt and<br />
extracellular signal-regulated kinase (ERK)1/2 phosphorylation but with slightly different kinetic pr<strong>of</strong>iles in the two cell lines.<br />
Inhibition <strong>of</strong> the phosphoinositide 3-kinase (PI3K)/Akt pathway by the PI3K inhibitor, LY294002, markedly suppressed HGFstimulated<br />
invasion <strong>of</strong> both CCA cell lines, and inhibition <strong>of</strong> the ERK pathway by U0126 suppressed HGF-induced invasion <strong>of</strong><br />
the KKU-M213 cell line but had a moderate effect on HuCCA-1 cells. CONCLUSION: These data indicate that HGF promotes<br />
CCA cell invasiveness through dys-localization <strong>of</strong> E-cadherin and induction <strong>of</strong> cell motility by distinct signaling pathways<br />
depending on cell line type. © <strong>2010</strong> Baishideng. All rights reserved.<br />
No.790<br />
Author (s) : Meng, X., Tancharoen, S., Kawahara, K.-I., Nawa, Y., Taniguchi, S., Hashiguchi, T., Maruyama, I.<br />
Title : 1,5-Anhydroglucitol attenuates cytokine release and protects mice with type 2 diabetes from inflammatory reactions<br />
Source : <strong>International</strong> Journal <strong>of</strong> Immunopathology and Pharmacology, Volume 23, Issue 1, January <strong>2010</strong>, Pages 105-119<br />
Document Type : Article<br />
Keywords : 1,5-Anhydroglucitol (1,5-AG); NF-κB; Proinflammatory cytokine; Type 2 diabetes<br />
<strong>Abstract</strong> : 1,5-anhydrogIucitol (1,5-AG) decreases in diabetic patients and is used as a marker <strong>of</strong> glycemic control. Type 2 diabetic<br />
patients are susceptibile to lipopolysaccharides (LPS), which stimulate macrophages to release large quantities <strong>of</strong> tumor<br />
necrosis factor (TNF)-α and interleukin (IL)-6. This study examines the effects <strong>of</strong> 1,5-AG on lung inflammation induced by LPS<br />
and consequent systemic inflammation to determine whether the decrease <strong>of</strong> 1,5-AG concentration induces susceptibility to<br />
LPS. Before the challenge with LPS (1 mg/kg in vivo and 500 ng/ml in vitro), we pretreated db/db mice and RAW264.7 cells<br />
with 1,5-AG at 38.5 mg/kg and 500 μg/mI, respectively. The levels <strong>of</strong> IL-6, TNF-α, macrophage chemoattractant protein<br />
(MCP)-1 and IL-1β in the serum and in the cell supernatants were measured. We also measured macrophage recruitment and<br />
the expression <strong>of</strong> inducible nitric oxide synthase (iNOS) in pulmonary tissues. We found that 1,5-AG attenuated serum<br />
cytokine release and protected db/db mice from LPS-induced pulmonary inflammation. In addition, 1,5-AG suppressed<br />
cytokine release and iNOS expression by suppressing Akt/NF-κB activity in RAW264.7 cells. These results suggest that 1,5-AG<br />
may be a mediator in, as well as marker for diabetes, and 1,5-AG intake may confer tolerance to LPS in patients with type 2<br />
diabetes. © by Biolife, s.a.s.<br />
No.791<br />
Author (s) : Mensing, J.P., Lomas, T., Kerdcharoen, T., Tuantranonta, A.<br />
Title : Inkjet-printed optical gas sensor based on metallo-phthalocyanine layers<br />
Source : ECTI-CON <strong>2010</strong> - The <strong>2010</strong> ECTI <strong>International</strong> Conference on Electrical Engineering/Electronics, Computer,<br />
Telecommunications and Information Technology, <strong>2010</strong>, Article number 5491560, Pages 966-969<br />
Document Type : Conference Paper<br />
Keywords : Absorbances, Analytes, Different solvents, Dynamic gas flow, Ink jet, Ink systems, Iso-propanols, Optical gas sensors, Printing<br />
performance, Solvent mixtures; Acetone, Information technology, Ink, Nitrogen compounds, Printing, Sensors, Surface active<br />
agents; Optical properties<br />
<strong>Abstract</strong> : Copper(II)-2,9,16,23-tetra-tertbutyl-29H,31H-phthalocyanine (CuTTBPc) containing inks based on different solvents and solvent<br />
mixtures are prepared. Surfactant and different polymers have been added to the ink systems in order to optimize the<br />
printing performance. Printability and printing performance have been evaluated. Furthermore the optical properties, namely<br />
absorbance in the UV/Vis range, <strong>of</strong> the printed films have been investigated in order to assess the applicability <strong>of</strong> ink jet<br />
printed phthalocyanine thin-films as transducers for optical sensors. Basic sensing experiments were performed under<br />
dynamic gas flow conditions, employing acetone and isopropanol as analytes.<br />
No.792<br />
Author (s) : Methasate, A., Trakarnsanga, A., Akaraviputh, T., Chinsawangwathanakol, V., Lohsiriwat, D.<br />
Title : Lymph node metastasis in gastric cancer: Result <strong>of</strong> D2 dissection<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 3, March <strong>2010</strong>, Pages 310-317<br />
Document Type : Article<br />
Keywords : D2 dissection; Gastrectomy; Gastric cancer<br />
<strong>Abstract</strong> : Background: Extent <strong>of</strong> lymph node dissection still remains one <strong>of</strong> the most controversial issues regarding radical gastrectomy.<br />
Knowledge <strong>of</strong> the pattern and incidence <strong>of</strong> lymph node metastasis may help to define the optimal extent <strong>of</strong> lymph node<br />
dissection. Material and Method: The authors analyzed lymph node metastasis and survival rate in 130 consecutive gastric<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
cancer patients who underwent radical gastrectomy with D2 dissection between June 2001 and October, 2008 at the<br />
Department <strong>of</strong> Surgery, Faculty <strong>of</strong> Medicine, Siriraj Hospital, <strong>Mahidol</strong> <strong>University</strong>, Thailand. Results: For N staging, 28.5% <strong>of</strong> the<br />
patients were N0 while N1 was 40% and N2 was 31.5%. 44% <strong>of</strong> the patients with lymph node positive had metastasis up to<br />
group 2 lymph nodes. The patients with node positive had 5 year survival <strong>of</strong> 39% while the patients with node negative had<br />
survival <strong>of</strong> 73% (p = 0.003). Tumor at the middle part <strong>of</strong> the stomach had the most widespread lymph node metastasis<br />
compared to other regions. Lymph node group 7, 8 and 9 had a high incidence <strong>of</strong> lymph node metastasis especially for distal<br />
cancer while lymph node group 10, 11, 12 had lower incidence <strong>of</strong> metastasis. No mortality was seen in the present study.<br />
Conclusion: N staging, number <strong>of</strong> metastatic node > 5 and angiolymphatic invasion were the lymph node related factors<br />
contributing to survival. For radical gastrectomy, D2 dissection is required for adequate clearance <strong>of</strong> metastatic lymph nodes,<br />
which can be done without mortality.<br />
No.793<br />
Author (s) : Methasate, A., Trakarnsanga, A., Akaraviputh, T., Chinsawangwathanakol, V., Lohsiriwat, D.<br />
Title : Radical esophagectomy for esophageal cancer: Results in Thai patients<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 11, November <strong>2010</strong>, Pages 1256-1261<br />
Document Type : Article<br />
Keywords : Esophageal cancer; Lymph node dissection; Radical esophagectomy<br />
<strong>Abstract</strong> : Background: Radical esophagectomy was reported to have prolonged survival in patients with esophageal cancer. However, it<br />
is associated with high morbidity and mortality. Controversy still exists regarding value <strong>of</strong> radical esophagectomy. Material and<br />
Method: The authors analyzed the results, including complications and survival in 68 consecutive patients with esophageal<br />
cancer who were treated with transthoracic radical esophagectomy at the Department <strong>of</strong> Surgery, Siriraj hospital, <strong>Mahidol</strong><br />
<strong>University</strong> between June 2002 and June 2008. Results: There were 57 males and eight females with a mean age <strong>of</strong> 59.28 +<br />
11.25 years. Regarding T staging, 81.5% <strong>of</strong> the patients were in T3 stage while 1.5% <strong>of</strong> the patients had T1 stage, 15.4% had<br />
T2 stage, and 1.5% had T4 stage. Lymph node metastasis (N1) was found in 63.1% <strong>of</strong> the patients and N0 was in 36.9%. Most<br />
<strong>of</strong> the patients were in advanced stages with 53.8% in stage III, 44.6% in stage II, and 1.5% in stage I. The 5-year survival rate<br />
<strong>of</strong> the patients with node positive was significantly lower than the patients with node negative (p = 0.018). The survival was<br />
significantly better in stage II compared to stage III (p = 0.012). Overall 5-year survival rate was 28.5%. Most common<br />
complications were from pulmonary causes (22.1%), anastomotic leakage (8.8%), and wound infection (8.8%). Mortality rate<br />
was 4.41%. Conclusion: Radical esophagectomy was associated with relatively low mortality and acceptable survival. It should<br />
be considered in surgical treatment <strong>of</strong> patients with esophageal cancer.<br />
No.794<br />
Author (s) : Millet, Y.A., Danna, C.H., Clay, N.K., Songnuan, W., Simon, M.D., Werck-Reichhart, D., Ausubel, F.M.<br />
Title : Innate immune responses activated in Arabidopsis roots by microbe-associated molecular patterns<br />
Source : Plant Cell, Volume 22, Issue 3, March <strong>2010</strong>, Pages 973-990<br />
Document Type : Article<br />
Keywords : Arabidopsis protein, callose, camalexin, chitin, cyclopentane derivative, cytochrome P450, ethylene, ethylene<br />
derivative, glucan, glycosidase, indole derivative, jasmonic acid, oxylipin, PEN2 protein, Arabidopsis, peptidoglycan, plant<br />
RNA, salicylic acid, thiazole derivative; Arabidopsis, article, flagellum, genetics, host pathogen<br />
interaction, immunology, metabolism, plant root, Pseudomonas, signal transduction, transgenic plant; Arabidopsis, Arabidopsis<br />
Proteins, Chitin, Cyclopentanes, Cytochrome P-450 Enzyme System, Ethylenes, Flagella, Glucans, Host-Pathogen<br />
Interactions, Indoles, N-Glycosyl Hydrolases, Oxylipins, Peptidoglycan, Plant Roots, Plants, Genetically<br />
Modified, Pseudomonas, RNA, Plant, Salicylic Acid, Signal Transduction, Thiazoles; Arabidopsis, Arabidopsis<br />
thaliana, Pseudomonas syringae<br />
<strong>Abstract</strong> : Despite the fact that roots are the organs most subject to microbial interactions, very little is known about the response <strong>of</strong><br />
roots to microbe-associated molecular patterns (MAMPs). By monitoring transcriptional activation <strong>of</strong> b-glucuronidase reporters<br />
and MAMP-elicited callose deposition, we show that three MAMPs, the flagellar peptide Flg22, peptidoglycan, and chitin,<br />
trigger a strong tissue-specific response in Arabidopsis thaliana roots, either at the elongation zone for Flg22 and<br />
peptidoglycan or in the mature parts <strong>of</strong> the roots for chitin. Ethylene signaling, the 4-methoxy-indole-3-ylmethylglucosinolate<br />
biosynthetic pathway, and the PEN2 myrosinase, but not salicylic acid or jasmonic acid signaling, play major roles in this<br />
MAMP response. We also show that Flg22 induces the cytochrome P450 CYP71A12-dependent exudation <strong>of</strong> the phytoalexin<br />
camalexin by Arabidopsis roots. The phytotoxin coronatine, an Ile-jasmonic acid mimic produced by Pseudomonas syringae<br />
292
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
pathovars, suppresses MAMP-activated responses in the roots. This suppression requires the E3 ubiquitin ligase COI1 as well as<br />
the transcription factor JIN1/MYC2 but does not rely on salicylic acid-jasmonic acid antagonism. These experiments<br />
demonstrate the presence <strong>of</strong> highly orchestrated and tissue-specific MAMP responses in roots and potential pathogenencoded<br />
mechanisms to block these MAMP-elicited signaling pathways. © <strong>2010</strong> American Society <strong>of</strong> Plant Biologists.<br />
No.795<br />
Author (s) : Minami, A., Chaicumpa, W., Chongsa-Nguan, M., Samosornsuk, S., Monden, S., Takeshi, K., Makino, S.-i., Kawamoto, K.<br />
Title : Prevalence <strong>of</strong> foodborne pathogens in open markets and supermarkets in Thailand<br />
Source : Food Control, Volume 21, Issue 3, March <strong>2010</strong>, Pages 221-226<br />
Document Type : Article<br />
Keywords : Foodborne pathogens distribution; Listeria monocytogenes; Retail market contamination; Salmonella; Thailand<br />
<strong>Abstract</strong> : This study was conducted in Thailand (Bangkok and Pathum Thani provinces), from June 2006 to July 2007, in order to assess<br />
the prevalence <strong>of</strong> Listeria monocytogenes, Escherichia coli O157, Salmonella, Shigella and Vibrio parahaemolyticus in foods.<br />
Retail raw meats and seafood, including chicken (n = 109), pork (n = 80), beef (n = 108), shrimp (n = 43) and oysters (n = 48),<br />
from open markets and supermarkets were analyzed. Salmonella was found in 22 <strong>of</strong> 61 (36%) open market samples (48% <strong>of</strong><br />
chicken, none <strong>of</strong> pork and beef, and 53% <strong>of</strong> shrimp) and in 12 <strong>of</strong> 75 (16%) samples from supermarkets (57%, 12%, 24%, 0%<br />
respectively). However, a small number <strong>of</strong> L. monocytogenes were isolated, where 6 <strong>of</strong> 217 (3%) were samples from open<br />
markets (6% <strong>of</strong> chicken and 3% <strong>of</strong> pork) and 17 <strong>of</strong> 171 (10%) were from supermarkets (3% <strong>of</strong> beef, 4% <strong>of</strong> chicken, and 32% <strong>of</strong><br />
pork). In both markets, L. monocytogenes was not detected from shrimps, neither from oysters. E. coli O157, Shigella and<br />
tdh-positive V. parahaemolyticus were not isolated in this collection. Several Salmonella and L. monocytogenes isolates were<br />
multidrug-resistant. Both markets would need better assessment, since multidrug-resistant strains have been isolated and<br />
they may lead to therapeutic failure. © 2009 Elsevier Ltd. All rights reserved.<br />
No.796<br />
Author (s) : Mingkumlert, S., Vanasant, T., Limroongreungrat, W., Chanchaiyakul, R., Wang, Y.T.<br />
Title : Effect Of Core Strength And Stability Training On The X-factor Stretch In Amateur Golfers<br />
Source : MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, Volume: 42, Issue: 5, Supplement: 1, Pages: 677-678, Meeting <strong>Abstract</strong>:<br />
2584, Published: MAY <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.797<br />
Author (s) : Mingthong, P., Veerasai, W., Aeimbhu, A.<br />
Title : Fabrication <strong>of</strong> titanium oxide nanotube arrays on titanium implants: The effect <strong>of</strong> electrolytes conditions<br />
Source : IFMBE Proceedings, Volume 31 IFMBE, <strong>2010</strong>, Pages 1208-1211<br />
Document Type : Conference Paper<br />
Keywords : Anodic oxidation; Biomaterial; Titanium; Titanium oxide nanotubes<br />
<strong>Abstract</strong> : The primary aim <strong>of</strong> this study is to observe the influence <strong>of</strong> an applied voltages and a concentration <strong>of</strong> electrolyte on anodic<br />
oxide layer properties such as morphology, wettability and microstructural. First, sample was anodised under different<br />
voltages ranging from 10-40 volts in 0.1M H2SO 4/0.075wt% HF. Anodised samples were characterised by Scanning Electron<br />
Microscope (SEM) and X-ray Diffraction technique (XRD). It was observed that an applied voltage at 20 volts, an<br />
interconnected pore network can be fabricated. Second, sample was prepared in 0.1M H2SO4 with the concentration <strong>of</strong> HF<br />
range from 0.075 -0.5 wt% at the applied potential <strong>of</strong> 20 volts. Based on experimental results, the microstructure <strong>of</strong> anodic<br />
oxide layers depends strongly on the formation potential and the HF concentration. Nanopores can be formed under a range<br />
<strong>of</strong> experimental conditions. Moreover, it was found that titanium oxide nanotube arrays are formed in 0.1M H2SO4/0.4wt%<br />
HF. XRD patterns <strong>of</strong> anodised samples showed the tubes crystallised in a coexistent <strong>of</strong> titanium and titanium oxide phases.<br />
The anodised surfaces are more hydrophobic than the untreated surface. © <strong>2010</strong> <strong>International</strong> Federation for Medical and<br />
Biological Engineering.<br />
No.798<br />
Author (s) : Miotto, O., Heiny, A.T., Albrecht, R., García-Sastre, A., Tan, T.W., August, J.T., Brusic, V.<br />
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Title : Complete-proteome mapping <strong>of</strong> human influenza A adaptive mutations: Implications for human transmissibility <strong>of</strong> zoonotic<br />
strains<br />
Source : PLoS ONE, Volume 5, Issue 2, 3 February <strong>2010</strong>, Article number e9025<br />
Document Type : Article<br />
Keywords : hemagglutinin, ribonucleoprotein, proteome, virus protein; amino acid sequence, article, comparative study, controlled<br />
study, Influenza virus A H5N1, nonhuman, virus isolation, virus mutation, virus strain, adaptation, animal, avian<br />
influenza, bird, classification, disease transmission, genetics, horse, human, influenza, Influenza virus<br />
A, metabolism, methodology, molecular genetics, mutation, orthomyxovirus infection, protein<br />
database, proteomics, sequence homology, swine, time, virology, zoonosis; Aves, Influenza A virus, Suidae; Adaptation,<br />
Physiological, Amino Acid Sequence, Animals, Birds, Databases, Protein, Horses, Humans, Influenza A virus, Influenza A Virus,<br />
H5N1 Subtype, Influenza in Birds, Influenza, Human, Molecular Sequence Data, Mutation, Orthomyxoviridae<br />
Infections, Proteome, Proteomics, Sequence Homology, Amino Acid, Swine, Time Factors, Viral Proteins, Zoonoses<br />
<strong>Abstract</strong> : Background: There is widespread concern that H5N1 avian influenza A viruses will emerge as a pandemic threat, if they<br />
become capable <strong>of</strong> human-to-human (H2H) transmission. Avian strains lack this capability, which suggests that it requires<br />
important adaptive mutations. We performed a large-scale comparative analysis <strong>of</strong> proteins from avian and human strains, to<br />
produce a catalogue <strong>of</strong> mutations associated with H2H transmissibility, and to detect their presence in avian isolates.<br />
Methodology/Principal Findings:We constructed a dataset <strong>of</strong> influenza A protein sequences from 92,343 public database<br />
records. Human and avian sequence subsets were compared, using a method based on mutual information, to identify<br />
characteristic sites where human isolates present conserved mutations. The resulting catalogue comprises 68 characteristic<br />
sites in eight internal proteins. Subtype variability prevented the identification <strong>of</strong> adaptive mutations in the hemagglutinin and<br />
neuraminidase proteins. The high number <strong>of</strong> sites in the ribonucleoprotein complex suggests interdependence between<br />
mutations in multiple proteins. Characteristic sites are <strong>of</strong>ten clustered within known functional regions, suggesting their<br />
functional roles in cellular processes. By isolating and concatenating characteristic site residues, we defined adaptation<br />
signatures, which summarize the adaptive potential <strong>of</strong> specific isolates. Most adaptive mutations emerged within three<br />
decades after the 1918 pandemic, and have remained remarkably stable thereafter. Two lineages with stable internal protein<br />
constellations have circulated among humans without reassorting. On the contrary, H5N1 avian and swine viruses reassort<br />
frequently, causing both gains and losses <strong>of</strong> adaptive mutations. Conclusions: Human host adaptation appears to be complex<br />
and systemic, involving nearly all influenza proteins. Adaptation signatures suggest that the ability <strong>of</strong> H5N1 strains to infect<br />
humans is related to the presence <strong>of</strong> an unusually high number <strong>of</strong> adaptive mutations. However, these mutations appear<br />
unstable, suggesting low pandemic potential <strong>of</strong> H5N1 in its current form. In addition, adaptation signatures indicate that<br />
pandemic H1N1/09 strain possesses multiple human-transmissibility mutations, though not an unusually high number with<br />
respect to swine strains that infected humans in the past. Adaptation signatures provide a novel tool for identifying zoonotic<br />
strains with the potential to infect humans. © <strong>2010</strong> Miotto et al.<br />
No.799<br />
Author (s) : Miriyala, S., Thipakkom, C., Xu, Y., Prachayasittikul, V., Noel, T., St Clair, D.<br />
Title : 4-hydroxy-2-nonenal Mediates AIFm2 Release from Mitochondria: An Insight into the Mechanism <strong>of</strong> Oxidative Stress Mediated<br />
Retrograde Signaling<br />
Source : FREE RADICAL BIOLOGY AND MEDICINE, Volume: 49, Supplement: 1, Pages: S170-S170, DOI:<br />
10.1016/j.freeradbiomed.<strong>2010</strong>.10.482, Published: <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.800<br />
Author (s) : Mischak, H., Kolch, W., Aivaliotis, M., Bouyssie, D., Court, M., Dihazi, H., Dihazi, GH., Franke, J., Garin, J., de Peredo, AG., Iph<strong>of</strong>er,<br />
A., Jansch, L., Lacroix, C., Makridakis, M., Masselon, C., Metzger, J., Monsarrat, B., Mrug, M., Norling, M., Novak, J., Pich, A., Pitt,<br />
A., Bongcam-Rudl<strong>of</strong>f, E., Siwy, J., Suzuki, H., Thongboonkerd, V., Wang, LS., Zoidakis, J., Zurbig, P., Schanstra, JP., Vlahou, A.<br />
Title : Comprehensive human urine standards for comparability and standardization in clinical proteome analysis<br />
Source : PROTEOMICS CLINICAL APPLICATIONS, Volume: 4, Issue: 4, Pages: 464-478, DOI: 10.1002/prca.200900189, Published: APR<br />
<strong>2010</strong><br />
Document Type : Article<br />
294
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : Clinical proteomics; Proteome; Standard; Urine<br />
<strong>Abstract</strong> : Purpose: Urine proteomics is emerging as a powerful tool for biomarker discovery. The purpose <strong>of</strong> this study is the<br />
development <strong>of</strong> a well-characterized "real life" sample that can be used as reference standard in urine clinical proteomics<br />
studies. Experimental design: We report on the generation <strong>of</strong> male and female urine samples that are extensively<br />
characterized by different platforms and methods (CE-MS, LC-MS, LC-MS/MS, 1-D gel analysis in combination with nano-LC<br />
MS/MS (using LTQ-FT ultra), and 2-DE-MS) for their proteome and peptidome. In several cases analysis involved a definition <strong>of</strong><br />
the actual biochemical entities, i.e. proteins/peptides associated with molecular mass and detected PTMs and the relative<br />
abundance <strong>of</strong> these compounds. Results: The combination <strong>of</strong> different technologies allowed coverage <strong>of</strong> a wide mass range<br />
revealing the advantages and complementarities <strong>of</strong> the different technologies. Application <strong>of</strong> these samples in "interlaboratory"<br />
and "inter-platform" data comparison is also demonstrated. Conclusions and clinical relevance: These wellcharacterized<br />
urine samples are freely available upon request to enable data comparison especially in the context <strong>of</strong><br />
biomarker discovery and validation studies. It is also expected that they will provide the basis for the comprehensive<br />
characterization <strong>of</strong> the urinary proteome.<br />
No.801<br />
Author (s) : Mitrevski, B.S., Wilairat, P., Marriott, P.J.<br />
Title : Comprehensive two-dimensional gas chromatography improves separation and identification <strong>of</strong> anabolic agents in doping<br />
control<br />
Source : Journal <strong>of</strong> Chromatography A, Volume 1217, Issue 1, 1 January <strong>2010</strong>, Pages 127-135<br />
Document Type : Article<br />
Keywords : Anabolic agents; Comprehensive two-dimensional gas chromatography; Doping control; Full scan analysis; GC ×<br />
GC; Similarity matching; Time-<strong>of</strong>-flight mass spectrometry<br />
<strong>Abstract</strong> : The application <strong>of</strong> comprehensive two-dimensional gas chromatography coupled to time-<strong>of</strong>-flight mass spectrometry (GC ×<br />
GC-TOFMS) for the analysis <strong>of</strong> six anabolic agents (AAs) in doping control is investigated in this work. A non-polar-polar<br />
column configuration with 0.2 μm film thickness (df) second dimension (2D) column was employed, <strong>of</strong>fering much better<br />
spread <strong>of</strong> the components on 2D when compared to the alternative 0.1 μm df2D column. The proposed method was tested<br />
on the "key" AA that the World Anti-Doping Agency (WADA) had listed at the low ng mL-1 levels (clenbuterol, 19norandrosterone,<br />
epimethendiol, 17α-methyl-5α-androstane-3α,17β-diol, 17α-methyl-5β-androstane-3α,17β-diol and 3′-<br />
OH-stanozolol). The compounds were spiked in a blank urine extract obtained by solid-phase extraction, hydrolysis and<br />
liquid-liquid extraction; prior to analysis they were converted to the corresponding trimethylsilyl (TMS) derivatives. The limit<br />
<strong>of</strong> detection (LOD) was below or equal to the minimum required performance limit (MRPL) <strong>of</strong> 2 ng mL-1 defined by WADA,<br />
and the correlation coefficient was in the range from 0.995 to 0.999. The method allows choosing an ion from the full mass<br />
spectra which shows the least interference from the matrix and/or the best sensitivity; this can only be done if full scan mass<br />
spectral data are available. The advantage <strong>of</strong> GC × GC over classical one-dimensional GC (1D GC), in terms <strong>of</strong> separation<br />
efficiency and sensitivity, is demonstrated on a positive urine control sample at a concentration <strong>of</strong> 5 ng mL-1. The obtained<br />
similarity to the in-house created TOFMS spectra library at this level <strong>of</strong> concentration was in the range from 822 to 932 (on<br />
the scale from 0 to 999). Since full mass spectral information are recorded, the method allows the retro-search <strong>of</strong> non-target<br />
compounds or new "designer steroids", which cannot be detected with established GC-MS methods that use selected ion<br />
monitoring (SIM) mode. © 2009 Elsevier B.V. All rights reserved.<br />
No.802<br />
Author (s) : Mitrevski, B.S., Wilairat, P., Marriott, P.J.<br />
Title : Evaluation <strong>of</strong> World Anti-Doping Agency criteria for anabolic agent analysis by using comprehensive two-dimensional gas<br />
chromatography-mass spectrometry<br />
Source : Analytical and Bioanalytical Chemistry, Volume 396, Issue 7, April <strong>2010</strong>, Pages 2503-2511<br />
Document Type : Conference Paper<br />
Keywords : Anabolic-androgenic steroids (AAS); Deconvolution; GC×GC; Relative abundance ratio; Tolerance window; WADA criteria<br />
<strong>Abstract</strong> : This work presents the validation study <strong>of</strong> the comprehensive two-dimensional gas chromatography (GC×GC)-time-<strong>of</strong>-flight<br />
mass spectrometry method performance in the analysis <strong>of</strong> the key World Anti-Doping Agency (WADA) anabolic agents in<br />
doping control. The relative abundance ratio, retention time, identification and other method performance criteria have been<br />
tested in the GC×GC format to confirm that they comply with those set by WADA. Furthermore, tens <strong>of</strong> other components<br />
were identified with an average similarity <strong>of</strong> >920 (on the 0-999 scale), including 10 other endogenous sterols, and full mass<br />
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spectra <strong>of</strong> 5,000+ compounds were retained. The testosterone/epitestosterone ratio was obtained from the same run. A new<br />
dimension in doping analysis has been implemented by addressing separation improvement. Instead <strong>of</strong> increasing the<br />
method sensitivity, which is accompanied by making the detector increasingly "blind" to the matrix (as represented by<br />
selected ion monitoring mode, high-resolution mass spectrometry (MS) and tandem MS), the method capabilities have been<br />
improved by adding a new "separation" dimension while retaining full mass spectral scan information. Apart from the<br />
requirement for the mass spectral domain that a minimum <strong>of</strong> three diagnostic ions with relative abundance <strong>of</strong> 5% or higher in<br />
the MS spectra, all other WADA criteria are satisfied by GC×GC operation. The minimum <strong>of</strong> three diagnostic ions arises from<br />
the need to add some degree <strong>of</strong> specificity to the acquired mass spectrometry data; however, under the proposed full MS<br />
scan method, the high MS similarity to the reference compounds <strong>of</strong>fers more than the required three diagnostic ions for an<br />
unambiguous identification. This should be viewed as an extension <strong>of</strong> the present criteria to a full-scan MS method. © <strong>2010</strong><br />
Springer-Verlag.<br />
No.803<br />
Author (s) : Mitrpanont, J.L., Fugkeaw, S., Pachimkul, T., Sawangphol, W.<br />
Title : Designing intelligent Web-based e-learning based on Web 2.0 and agents-based model<br />
Source : Proceedings <strong>of</strong> the IADIS <strong>International</strong> Conference e-Learning <strong>2010</strong>, Part <strong>of</strong> the IADIS Multi Conference on Computer Science<br />
and Information Systems <strong>2010</strong>, MCCSIS <strong>2010</strong>, Volume 2, <strong>2010</strong>, Pages 130-134<br />
Document Type : Conference Paper<br />
Keywords : Agents; E-learning web-based design; Web 2.0; Web service<br />
<strong>Abstract</strong> : In this paper, we propose the E-Learning Web-based Design Model based on Web Service and Web 2.0 model, extending<br />
Moodle open s<strong>of</strong>tware to construct additional supporting features <strong>of</strong> e-Learning system. The basic features <strong>of</strong> Moodle are<br />
adopted and extended to support advanced functions <strong>of</strong> the e-learning. To this end, Web 2.0 renders social network features<br />
and provides a promising channel to students, instructors, and administrators. Therefore, the customized Moodle system and<br />
Web 2.0 E-learning are integrated to provide a more accessible and fine-grained design <strong>of</strong> E-Learning system. To handle the<br />
multiple users using several services, we apply agents system to serve the tasks <strong>of</strong> each service cooperatively. In addition, we<br />
accommodate user feedback management (UFM) function to improve the maintenance process and the functionality <strong>of</strong> e-<br />
Learning system by allowing users to propose any ideas about their view on the E-learning system they want via the social<br />
networking channel. To incorporate the feedback and requirements from users, such information is retained and analyzed to<br />
generate the recommendation pr<strong>of</strong>iles which will be used for further improvement <strong>of</strong> design and performance <strong>of</strong> the system.<br />
© <strong>2010</strong> IADIS.<br />
No.804<br />
Author (s) : Modchang, C., Nadkarni, S., Bartol, T.M., Triampo, W., Sejnowski, T.J., Levine, H., Rappel, W.-J.<br />
Title : A comparison <strong>of</strong> deterministic and stochastic simulations <strong>of</strong> neuronal vesicle release models<br />
Source : Physical Biology, Volume 7, Issue 2, <strong>2010</strong>, Article number 026008<br />
Document Type : Article<br />
Keywords : calcium; algorithm, article, biological model, comparative study, computer simulation, metabolism, Monte Carlo<br />
method, nerve cell, statistics, synapse; Algorithms, Calcium, Computer Simulation, Models, Neurological, Monte Carlo<br />
Method, Neurons, Stochastic Processes, Synapses<br />
<strong>Abstract</strong> : We study the calcium-induced vesicle release into the synaptic cleft using a deterministic algorithm and MCell, a Monte Carlo<br />
algorithm that tracks individual molecules. We compare the average vesicle release probability obtained using both<br />
algorithms and investigate the effect <strong>of</strong> the three main sources <strong>of</strong> noise: diffusion, sensor kinetics and fluctuations from the<br />
voltage-dependent calcium channels (VDCCs). We find that the stochastic opening kinetics <strong>of</strong> the VDCCs are the main<br />
contributors to differences in the release probability. Our results show that the deterministic calculations lead to reliable<br />
results, with an error <strong>of</strong> less than 20%, when the sensor is located at least 50 nm from the VDCCs, corresponding to<br />
microdomain signaling. For smaller distances, i.e. nanodomain signaling, the error becomes larger and a stochastic algorithm is<br />
necessary. © <strong>2010</strong> IOP Publishing Ltd.<br />
No.805<br />
Author (s) : Modhiran, N., Kalayanarooj, S., Ubol, S.<br />
Title : Subversion <strong>of</strong> innate defenses by the interplay between DENV and pre-existing enhancing antibodies: TLRs signaling collapse<br />
Source : PLoS Neglected Tropical Diseases, Volume 4, Issue 12, December <strong>2010</strong>, Article number e924, Pages 1-12<br />
296
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Document Type : Article<br />
Keywords : beta interferon, Fc receptor, Fc receptor IIa, immunoglobulin enhancer binding protein, myeloid differentiation factor 88, toll<br />
like receptor, virus antibody; antigen antibody complex, article, child, controlled study, cytokine production, dengue, Dengue<br />
virus, down regulation, gene expression regulation, human, human cell, immunoblotting, innate<br />
immunity, nonhuman, peripheral blood mononuclear cell, preschool child, protein expression, protein function, reverse<br />
transcription polymerase chain reaction, school child, signal transduction, virus immunity<br />
<strong>Abstract</strong> : Background: The phenomenon <strong>of</strong> antibody dependent enhancement as a major determinant that exacerbates disease<br />
severity in DENV infections is well accepted. While the detailed mechanism <strong>of</strong> antibody enhanced disease severity is unclear,<br />
evidence suggests that it is associated with both increased DENV infectivity and suppression <strong>of</strong> the type I IFN and proinflammatory<br />
cytokine responses. Therefore, it is imperative for us to understand the intracellular mechanisms altered during<br />
ADE infection to decipher the mechanism <strong>of</strong> severe pathogenesis. Methodology/Principal Findings:In this present work, qRT-<br />
PCR, immunoblotting and gene array analysis were conducted to determine whether DENV-antibody complex infection exerts<br />
a suppressive effect on the expression and/or function <strong>of</strong> the pathogen recognition patterns, focusing on the TLR-signaling<br />
pathway. We show here that FccRI and FccRIIa synergistically facilitated entry <strong>of</strong> DENV-antibody complexes into monocytic<br />
THP-1 cells. Ligation between DENV-antibody complexes and FcR not only down regulated TLRs gene expression but also up<br />
regulated SARM, TANK, and negative regulators <strong>of</strong> the NF-kB pathway, resulting in suppression <strong>of</strong> innate responses but<br />
increased viral production. These results were confirmed by blocking with anti-FcγRI or anti-FcγRIIa antibodies which reduced<br />
viral production, up-regulated IFN-β synthesis, and increased gene expression in the TLR-dependent signaling pathway. The<br />
negative impact <strong>of</strong> DENV-ADE infection on the TLR-dependent pathway was strongly supported by gene array screening which<br />
revealed that both MyD88-dependent and -independent signaling molecules were down regulated during DENV-ADE infection.<br />
Importantly, the same phenomenon was seen in PBMC <strong>of</strong> secondary DHF/DSS patients but not in PBMC <strong>of</strong> DF patients.<br />
Conclusions/Significance: Our present work demonstrates the mechanism by which DENV uses pre-existing immune mediators<br />
to defeat the principal activating pathway <strong>of</strong> innate defense resulting in suppression <strong>of</strong> an array <strong>of</strong> innate immune responses.<br />
Interestingly, this phenomenon specifically occurred during the severe form <strong>of</strong> DENV infection but not in the mild form <strong>of</strong><br />
disease. © <strong>2010</strong> Modhiran et al.<br />
No.806<br />
Author (s) : Monatrakul, P., Mungthin, M., Dondorp, A.M., Krudsood, S., Udomsangpetch, R., Wilairatana, P., White, N.J., Chotivanich, K.<br />
Title : Modulating effects <strong>of</strong> plasma containing anti-malarial antibodies on in vitro anti-malarial drug susceptibility in Plasmodium<br />
falciparum<br />
Source : Malaria Journal, Volume 9, Issue 1, <strong>2010</strong>, Article number 326<br />
Document Type : Article<br />
Keywords : antiserum, artesunate, hypoxanthine, quinine, antimalarial agent, artemisinin derivative, protozoon antibody; acute<br />
disease, antibody titer, antimalarial drug susceptibility, article, blood sampling, controlled study, dose response, drug<br />
effect, fungal strain, human, IC 50, in vitro study, incubation time, malaria falciparum, microscopy, parasite<br />
development, Plasmodium falciparum, chemistry, drug sensitivity, immunology, methodology, plasma; Antibodies,<br />
Protozoan, Antimalarials, Artemisinins, Humans, Inhibitory Concentration 50, Parasitic Sensitivity Tests, Plasma, Plasmodium<br />
falciparum, Quinine<br />
<strong>Abstract</strong> : Background: The efficacy <strong>of</strong> anti-malarial drugs is determined by the level <strong>of</strong> parasite susceptibility, anti-malarial drug<br />
bioavailability and pharmacokinetics, and host factors including immunity. Host immunity improves the in vivo therapeutic<br />
efficacy <strong>of</strong> anti-malarial drugs, but the mechanism and magnitude <strong>of</strong> this effect has not been characterized. This study<br />
characterized the effects <strong>of</strong> 'immune' plasma to Plasmodium falciparumon the in vitro susceptibility <strong>of</strong> P. falciparum to antimalarial<br />
drugs. Methods. Titres <strong>of</strong> antibodies against blood stage antigens (mainly the ring-infected erythrocyte surface antigen<br />
[RESA]) were measured in plasma samples obtained from Thai patients with acute falciparum malaria. 'Immune' plasma was<br />
selected and its effects on in vitro parasite growth and multiplication <strong>of</strong> the Thai P. falciparum laboratory strain TM267 were<br />
assessed by light microscopy. The in vitro susceptibility to quinine and artesunate was then determined in the presence and<br />
absence <strong>of</strong> 'immune' plasma using the 3H-hypoxanthine uptake inhibition method. Drug susceptibility was expressed as the<br />
concentrations causing 50% and 90% inhibition (IC50and IC90), <strong>of</strong> 3H-hypoxanthine uptake. Results. Incubation with 'immune'<br />
plasma reduced parasite maturation and decreased parasite multiplication in a dose dependent manner. 3H-hypoxanthine<br />
incorporation after incubation with 'immune' plasma was decreased significantly compared to controls (median [range]; 181.5<br />
[0 to 3,269] cpm versus 1,222.5 [388 to 5,932] cpm) (p= 0.001). As a result 'immune' plasma reduced apparent susceptibility to<br />
quinine substantially; median (range) IC506.4 (0.5 to 23.8) ng/ml versus 221.5 (174.4 to 250.4) ng/ml (p = 0.02), and also had a<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
borderline effect on artesunate susceptibility; IC500.2 (0.02 to 0.3) ng/ml versus 0.8 (0.2 to 2.3) ng/ml (p = 0.08). Effects were<br />
greatest at low concentrations, changing the shape <strong>of</strong> the concentration-effect relationship. IC 90values were not significantly<br />
affected; median (range) IC 90448.0 (65 to > 500) ng/ml versus 368.8 (261 to 501) ng/ml for quinine (p > 0.05) and 17.0<br />
(0.1 to 29.5) ng/ml versus 7.6 (2.3 to 19.5) ng/ml for artesunate (p = 0.4). Conclusions. 'Immune' plasma containing antimalarial<br />
antibodies inhibits parasite development and multiplication and increases apparent in vitro anti-malarial drug<br />
susceptibility <strong>of</strong> P. falciparum. The IC90was much less affected than the IC50measurement. © <strong>2010</strong> Monatrakul et al; licensee<br />
BioMed Central Ltd.<br />
No.807<br />
Author (s) : Mongkolchati, A., Thinkhamrop, B., Mo-Suwan, L., Chittchang, U., Choprapawon, C.<br />
Title : Prevalence and incidence <strong>of</strong> child stunting from birth to two years <strong>of</strong> life in Thai children: Based on the prospective cohort<br />
study <strong>of</strong> Thai children (PCTC)<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 12, December <strong>2010</strong>, Pages 1368-1378<br />
Document Type : Article<br />
Keywords : Child stunting; Children growth; Cohort study; Incidence; Prevalence<br />
<strong>Abstract</strong> : Objective: To determine the percentages <strong>of</strong> prevalence and incidence in child stunting at birth, 6, 12, 18, and 24 months <strong>of</strong><br />
age and to investigate the association between factors and child stunting outcome Material and Method: The Prospective<br />
Cohort Study <strong>of</strong> Thai Children (PCTC) was carried out during 2000-2002, data from five districts were examined, and<br />
anthropometric measurements were performed by the physician and research assistants. WHO's growth reference standard<br />
year 2005 was used. Results: Four thousand two hundred forty five children were included at the start <strong>of</strong> the present study <strong>of</strong><br />
which 3,898 were in the final analysis. The prevalence in child stunting presented an increasing percentage at birth 6, 12, 18,<br />
and 24 months was 6.0, 6.9, 9.5, 14.6, and 16.6%, and incidence indicated decreasing at birth,6,12,18, and 24 mon ths was 6.0,<br />
4.3, 4.1, 5.2, and 3.2% respectively. The GEE analysis showed that gender, mother height, mother education, income, and<br />
Nan-Hill Tribe areas were significantly correlated with child stunting (p < 0.001). Conclusion: Finding from the present study<br />
indicates that to reduce child stunting in Thailand in early infant's life, early nutritional interventions and quality antenatal<br />
care are vital.<br />
No.808<br />
Author (s) : Monteerarat, Y., Sakabe, S., Ngamurulert, S., Srichatraphimuk, S., Jiamtom, W., Chaichuen, K., Thitithanyanont, A., Permpikul, P.,<br />
Songserm, T., Puthavathana, P., Nidom, C.A., Mai, L.Q., Iwatsuki-Horimoto, K., Kawaoka, Y., Auewarakul, P.<br />
Title : Induction <strong>of</strong> TNF-α in human macrophages by avian and human influenza viruses<br />
Source : Archives <strong>of</strong> Virology, 155 (8), pp. 1273-1279.<br />
Document Type : Article<br />
Keywords : NS protein, influenza virus, tumor necrosis factor alpha, virus protein; animal, article, avian<br />
influenza, biosynthesis, bird, human, immunology, influenza, Influenza virus A, Influenza virus A H1N1, Influenza virus A<br />
H3N2, Influenza virus A H5N1, macrophage, virology; Animals, Birds, Humans, Influenza A virus, Influenza A Virus, H1N1<br />
Subtype, Influenza A Virus, H3N2 Subtype, Influenza A Virus, H5N1 Subtype, Influenza in Birds, Influenza,<br />
Human, Macrophages, Tumor Necrosis Factor-alpha, Viral Nonstructural Proteins; Aves, Avian influenza virus, human influenza<br />
virus, Orthomyxoviridae<br />
<strong>Abstract</strong> : The highly pathogenic avian influenza virus H5N1 is known to induce high level <strong>of</strong> tumor necrosis factor α (TNF-α) from<br />
primary macrophages. However, it is still unclear whether current H5N1 strains also induce high TNF-α production, as most <strong>of</strong><br />
the data were derived from extinct clade 0 H5N1 strain. Here, we show that current clade 1 and 2 H5N1 strains induce<br />
variable levels <strong>of</strong> TNF-α that are not necessarily higher than those induced by seasonal influenza viruses. The result suggests<br />
that hyper-induction <strong>of</strong> TNF-α in human macrophages is not always associated with a highly pathogenic phenotype. We<br />
further tested the contribution <strong>of</strong> the NS gene segment from H5N1 isolates to TNF-α induction by using reverse genetics.<br />
While NS conferred some variation in TNF-α induction when incorporated into an H1N1 virus genetic background, it did not<br />
affect TNF-α induction in an H5N1 virus genetic background, suggesting that other viral genes are involved. © <strong>2010</strong> Springer-<br />
Verlag.<br />
No.809<br />
Author (s) : Monteerarat, Y., Suptawiwat, O., Boonarkart, C., Uiprasertkul, M., Auewarakul, P., Viprakasit, V.<br />
Title : Inhibition <strong>of</strong> H5N1 highly pathogenic influenza virus by suppressing a specific sialyltransferase<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Source : Archives <strong>of</strong> Virology, Volume 155, Issue 6, <strong>2010</strong>, Pages 889-893<br />
Document Type : Article<br />
Keywords : beta galactoside alpha 2,3 sialyltransferase, beta-galactoside alpha-2,3-sialyltransferase, n acetylneuraminic<br />
acid, sialyltransferase, small interfering RNA, virus receptor; animal, article, bronchus, cell line, dog, down<br />
regulation, enzymology, genetics, human, Influenza virus A H5N1, lung, metabolism, pathogenicity, pharynx, respiratory<br />
system, RNA interference, trachea; Animals, Bronchi, Cell Line, Dogs, Down-Regulation, Humans, Influenza A Virus, H5N1<br />
Subtype, Lung, N-Acetylneuraminic Acid, Pharynx, Receptors, Virus, Respiratory System, RNA Interference, RNA, Small<br />
Interfering, Sialyltransferases, Trachea; Avian influenza virus, Orthomyxoviridae<br />
<strong>Abstract</strong> : Avian influenza viruses preferentially use α2,3-linked sialic acid as a receptor for binding and entry into target cells. The sialic<br />
acid is the terminal residue <strong>of</strong> various types <strong>of</strong> glycan. There are two major types <strong>of</strong> α2,3-linked sialic acid differing in the<br />
penultimate bond: Neu5Acα2-3Galβ1-3GalNAc and Neu5Acα2-3Galβ1-4GlcNAc. In the human airway, while Neu5Acα2-<br />
3Galβ1-3GalNAc is present only in alveolar epithelial cells, the Neu5Acα2-3Galβ1-4GlcNAc is expressed in both the upper<br />
and lower airway. Previous data showed preferential binding <strong>of</strong> hemagglutinin from H5N1 highly pathogenic influenza virus to<br />
Neu5Acα2-3Galβ1-4GlcNAc. We further show here that suppression <strong>of</strong> this sialic acid by siRNA against a sialyltransferase,<br />
ST3GAL4, can inhibit H5N1 avian influenza virus infection and that this gene is abundantly expressed in human pharynx,<br />
trachea and bronchus. These data suggest that the ST3GAL4 gene is responsible for biosynthesis <strong>of</strong> the viral receptor and may<br />
play a crucial role in infection <strong>of</strong> H5N1 avian influenza virus in humans. © <strong>2010</strong> Springer-Verlag.<br />
No.810<br />
Author (s) : Monvises, A., Ruenwongsa, P., Panijpan, B., Sriwattanarothai, N.<br />
Title : A siamese fighting fish learning unit for cooperative learning among primary students<br />
Source : <strong>International</strong> Journal <strong>of</strong> Learning, Volume 17, Issue 5, <strong>2010</strong>, Pages 231-246<br />
Document Type : Article<br />
Keywords : Cooperative learning; Fighting fish; Learning center; Primary students<br />
<strong>Abstract</strong> : The study focused on the effects <strong>of</strong> the cooperative learning on students' achievement. A learning unit on the fighting fish<br />
living process was used as a case study. The participants, 30 grade-4 students, were randomly divided into five groups <strong>of</strong> six<br />
students each. A learning center on fighting fish was established and equipped with learning resources in the school. Students<br />
worked together in each topic which differed from group to group. After the activity each group shared their findings to the<br />
class. Following extensive discussion all students were expected to gain knowledge from both ingroup activity and activities<br />
<strong>of</strong> other groups. For the overall knowledge gained, the students were assessed both individually and as a group. The results<br />
showed improvement in the level <strong>of</strong> knowledge <strong>of</strong> the students from the first week to the third week. Results from their<br />
mind maps indicated that almost all students were able to conceptualize about various aspects <strong>of</strong> the life <strong>of</strong> the fighting fish.<br />
This can be explained by the self-accountability <strong>of</strong> individual students as well as their responsibility for the achievement <strong>of</strong><br />
their friends. The students showed positive attitude toward the cooperative learning. © Common Ground, Adisorn Monvises,<br />
Pintip Ruenwongsa, Bhinyo Panijpan, Namkang Sriwattanarothai.<br />
No.811<br />
Author (s) : Mookum, T., Wiwatanapataphee, B., Wu, Y.H.<br />
Title : Modeling <strong>of</strong> two-fluid flow and heat transfer with solidification in continuous steel casting process under electromagnetic<br />
force<br />
Source : <strong>International</strong> Journal <strong>of</strong> Pure and Applied Mathematics, Volume 63, Issue 2, <strong>2010</strong>, Pages 183-195<br />
Document Type : Article<br />
Keywords : Continuous steel casting process; Electromagnetic stirring; Heat transfer; Level set finite element method; Tw<strong>of</strong>luid flow<br />
<strong>Abstract</strong> : This paper is concerned with the two-fluid flow and heat transfer in the continuous steel casting process under<br />
electromagnetic (EM) force. The governing equations consist <strong>of</strong> the Navier-Stokes equations, the continuity equation, and the<br />
energy equation. The influence <strong>of</strong> the EM field on the flow pattern, the meniscus shape, and temperature distribution in the<br />
EM caster is modeled by the addition <strong>of</strong> the EM force and the surface tension force in the Navier-Stokes equations. The EM<br />
force is defined by the cross product <strong>of</strong> current density and magnetic flux density obtained from the Maxwell's equations. A<br />
surface tension force is a function <strong>of</strong> the level set function which can be solved from the level set equation. A complete set<br />
<strong>of</strong> governing equations is solved by the level set finite element method. The numerical results demonstrate that the EM field<br />
applied to the system has significant effect on the two-fluid flow, meniscus pr<strong>of</strong>ile, and temperature distribution. © <strong>2010</strong><br />
Academic <strong>Publications</strong>.<br />
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No.812<br />
Author (s) : Moonchai, S., Lenbury, Y., Triampo, W.<br />
Title : Cellular automata simulation modeling <strong>of</strong> HIV infection in Lymph Node and peripheral blood compartments<br />
Source : <strong>International</strong> Journal <strong>of</strong> Mathematics and Computers in Simulation, Volume 4, Issue 4, <strong>2010</strong>, Pages 124-134<br />
Document Type : Article<br />
Keywords : Cellular automata; Double compartments simulation; HIV proliferation<br />
<strong>Abstract</strong> : Acquired immune deficiency syndrome (AIDS) has been widely considered as the most devastating epidemic. To discover<br />
effective therapy for HIV infection, the dynamics <strong>of</strong> the virus-immune system in the human body have been the subject <strong>of</strong><br />
intense studies. Since the development <strong>of</strong> the disease typically exhibits a three phase evolution, that is, an acute phase<br />
(measured in days), a chronic phase (measured in weeks) and AIDS (measured in years), the use <strong>of</strong> ordinary or partial<br />
differential equations are inadequate in our attempt to describe the three different time scales in order to simulate the entire<br />
course <strong>of</strong> the HIV infection. Cellular automata simulation approach has become well known as a useful technique to<br />
investigate complex biomedical systems in situations where traditional methodologies are difficult or too costly to employ.<br />
So far, relatively simple cellular automata models have been proposed to simulate the dynamics <strong>of</strong> HIV infection in human.<br />
Most cellular automata models only considered viral proliferation in the lymph node. However, most clinical indications <strong>of</strong><br />
AIDS progression are based on blood data, because these data are most easily obtained. Since viral population circulates<br />
between lymph node and plasma, viral load in the two compartments are important for the description <strong>of</strong> HIV infection<br />
dynamics. We present here cellular automata simulations <strong>of</strong> a two-compartment model <strong>of</strong> HIV proliferation with delay.<br />
No.813<br />
Author (s) : Moonchai, S., Lenbury, Y., Triampo, W.<br />
Title : Multiple latticed cellular automata: HIV dynamics in coupled lymph node and peripheral blood compartments<br />
Source : <strong>International</strong> Conference on Applied Computer Science - Proceedings, <strong>2010</strong>, Pages 56-61<br />
Document Type : Conference Paper<br />
Keywords : Cellular automata; Double compartments simulation; HIV proliferation<br />
<strong>Abstract</strong> : Cellular automata simulation approach has become well known as a useful technique to investigate complex biomedical<br />
systems in situations where traditional methodologies are difficult or too costly to employ. So far, relatively simple cellular<br />
automata models have been proposed to simulate the dynamics <strong>of</strong> HIV infection in human. Most cellular automata models<br />
only considered viral proliferation in the lymph node. However, most clinical indications <strong>of</strong> AIDS progression are based on<br />
blood data, because these data are most easily obtained. Since viral population circulates between lymph node and plasma,<br />
viral load in the two compartments are important for the description <strong>of</strong> HIV infection dynamics. We present here cellular<br />
automata simulations <strong>of</strong> a two-compartment model <strong>of</strong> HIV proliferation with delay.<br />
No.814<br />
Author (s) : Moongkarndi, P., Srisawat, C., Saetun, P., Jantaravinid, J., Peerapittayamongkol, C., Soi-Ampornkul, R., Junnu, S., Sinchaikul, S.,<br />
Chen, S.-T., Charoensilp, P., Thongboonkerd, V., Neungton, N.<br />
Title : Protective effect <strong>of</strong> mangosteen extract against β-amyloid-induced cytotoxicity, oxidative stress and altered proteome in SK-<br />
N-SH cells<br />
Source : Journal <strong>of</strong> Proteome Research, Volume 9, Issue 5, 7 May <strong>2010</strong>, Pages 2076-2086<br />
Document Type : Article<br />
Keywords : Alzheimers disease; Amyloid; Cytotoxicity; Mangosteen extract; Proteome; Proteomics<br />
<strong>Abstract</strong> : β-amyloid (Aβ) plays a key role in the pathogenesis <strong>of</strong> Alzheimers disease (AD) by inducing neurotoxicity and cell death<br />
mainly through production <strong>of</strong> reactive oxygen species (ROS). Garcinia mangostana L. (mangosteen) has been recognized as a<br />
major source <strong>of</strong> natural antioxidants that could decrease ROS. However, its role in protection <strong>of</strong> Aβ-induced cytotoxicity and<br />
apoptosis in neuronal cells remains unclear. We therefore examined such a protective effect <strong>of</strong> mangosteen extract (ME) by<br />
evaluating cell viability using MTT test, ROS level, caspase-3 activity, and cellular proteome. Treating SK-N-SH cells with 5-20<br />
μM Aβ(1-42) for 24 h caused morphologically cytotoxic changes, decreased cell viability and increased ROS level, whereas<br />
preincubation with 50-400 μg/mL ME 30 min before the induction by Aβ(1-42) successfully prevented such cytotoxic effects<br />
in a dose-dependent manner (completely at 400 μg/mL). The Aβ-induced increase in caspase-3 activity was also preventable<br />
by 400 μg/mL ME. Proteomic analysis using 2-D gel electrophoresis (n = 5 gels/group) followed by mass spectrometry<br />
revealed 63 proteins whose levels were significantly altered by Aβ(1-42) induction. Interestingly, changes in 10 proteins were<br />
successfully prevented by the ME pretreatment. In summary, we report herein the significant protective effects <strong>of</strong> ME against<br />
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Aβ-induced cytotoxicity, increased ROS, and increased caspase activity in SK-N-SH cells. Moreover, proteomic analysis<br />
revealed some proteins that might be responsible for these protective effects by ME. Further characterizations <strong>of</strong> these<br />
proteins may lead to identification <strong>of</strong> novel therapeutic targets for successful prevention and/or decreasing the severity <strong>of</strong> AD.<br />
© <strong>2010</strong> American Chemical Society.<br />
No.815<br />
Author (s) : Moore, C.E.; Sengduangphachanh, A.; Thaojaikong, T.; Sirisouk, J.; Foster, D.; Phetsouvanh, R.; McGee, L.; Crook, D.W.; Newton,<br />
P.N.; Peacock, S.J.<br />
Title : Enhanced Determination <strong>of</strong> Streptococcus pneumoniae Serotypes Associated with Invasive Disease in Laos by Using a Real-<br />
Time Polymerase Chain Reaction Serotyping Assay with Cerebrospinal Fluid<br />
Source : AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, Volume: 83, Issue: 3, Pages: 451-457, DOI: 10.4269/ajtmh.<strong>2010</strong>.10-<br />
0225, Published: SEP <strong>2010</strong><br />
Document Type : Article<br />
Keywords : PNEUMOCOCCAL CONJUGATE VACCINE; PLACEBO-CONTROLLED-TRIAL; MULTIPLEX PCR; ANTIMICROBIAL SUSCEPTIBILITY;<br />
QUELLUNG REACTION; DEVELOPING-WORLD; DOUBLE-BLIND; CHILDREN; SURVEILLANCE; 6C<br />
<strong>Abstract</strong> : A prospective hospital-based study was undertaken to define the incidence <strong>of</strong> invasive pneumococcal disease (IPD) and<br />
circulating serotypes in Laos Of 10.799 patients with hemocultures and 353 patients with cerebrospinal fluid samples. 0 21%<br />
and 5 4%, respectively, were positive for Streptococcus pneumoniae. giving a total <strong>of</strong> 35 IPD patients We developed a realtime<br />
polymerase chain reaction to detect serotypes represented in the 13-valent pneumococcal vaccine A blinded<br />
evaluation comparing serotype as defined by the Quellung reaction versus the polymerase chain reaction demonstrated<br />
100% concordance The most frequent serotype (n = 33 patients) was 1 (n = 6), followed by serotypes 5, 6A/B/C, 14, and 23F.<br />
Serotypes represented in the 7-valent polysaccharide-protein conjugate vaccine (PCV-7) infected 39% <strong>of</strong> patients with 73%<br />
coverage for the PCV-10 and PCV-13 vaccines. Although the sample size is small, these data suggest that the PCV-7 vaccine<br />
may have relatively low efficacy in Laos Further studies are urgently needed to guide pneumococcal vaccine policy in Laos<br />
No.816<br />
Author (s) : Mophan, N., Vinitnantharat, S., Somsook, E.<br />
Title : Enhancing iron(III) solubility using cassava and arrowroot starch<br />
Source : ScienceAsia, Volume 36, Issue 2, June <strong>2010</strong>, Pages 172-173<br />
Document Type : Article<br />
Keywords : Iron oxide; Iron-saccharide; Iron-starch<br />
<strong>Abstract</strong> : Iron(III) is sparsely soluble in aqueous solutions even in an acidic condition. The solubility <strong>of</strong> iron(III) can be enhanced by<br />
complexing it with saccharides. Iron-dextran and iron-sucrose have been used to treat iron-deficiency anaemia. This work<br />
investigates the enhancement <strong>of</strong> the solubility <strong>of</strong> iron(III) in aqueous solution by complexing it with polysaccharides from<br />
locally available starch. Iron(III) was complexed with cassava and arrowroot starch and the state <strong>of</strong> iron(III) in the solutions<br />
was examined by UV-Vis spectrophotometry. The results showed that the solubility <strong>of</strong> iron(III) could be enhanced by cassava<br />
and arrowroot starch. It was asserted that these polysaccharides prevent the hydrolysis and precipitation <strong>of</strong> the iron(III) as<br />
iron-oxide. These finding might lead to an alternative treatment for iron-deficiency anaemia.<br />
No.817<br />
Author (s) : Morales Vargas, R.E., Ya-umphan, P., Phumala-Morales, N., Komalamisra, N., Dujardin, J.-P.<br />
Title : Climate associated size and shape changes in Aedes aegypti (Diptera: Culicidae) populations from Thailand<br />
Source : Infection, Genetics and Evolution, Volume 10, Issue 4, May <strong>2010</strong>, Pages 580-585<br />
Document Type : Article<br />
Keywords : Aedes aegypti; Climate; Dengue; Relative humidity; Shape; Size; Thailand<br />
<strong>Abstract</strong> : In spite <strong>of</strong> the adult body size variability <strong>of</strong> Aedes aegypti (Linnaeus) and its likely association with life history and vectorial<br />
capacity, the causes <strong>of</strong> size variation itself have been only partially identified. In particular, possible important factors such as<br />
climatic variation have not received much attention.The objective <strong>of</strong> this 2-year study was to describe from field collections<br />
the relationship <strong>of</strong> Ae. aegypti metric properties with available climatic data. The study took place in a dengue hyperendemic<br />
area <strong>of</strong> Thailand. Fourth instar larvae (L4) and pupae were collected from the same breeding places allowing the comparisons<br />
between seven successive collections, four in 2007 and three in 2008. Climatic data were relative humidity (RH) and<br />
temperature (T). They were considered for the periods covering either the pre-imaginal development or, assuming heritability<br />
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<strong>of</strong> size, the previous generation. The pre-imaginal period was further subdivided into embryonic and larval phases <strong>of</strong><br />
development. Size was estimated by traditional and geometric techniques, the latter based on 18 landmarks collected at the<br />
intersections <strong>of</strong> veins also allowing estimation <strong>of</strong> shape.The shape variation <strong>of</strong> the wing followed similar patterns as for size<br />
and was shown to be a passive allometric change. No significant correlation <strong>of</strong> size or shape could be disclosed with T. In<br />
contrast, significant correlation with RH was found during two periods <strong>of</strong> examination: (i) the period affecting the generation<br />
previous to the time <strong>of</strong> collection, suggesting possible selective mechanisms on genitors, and (ii) the one occurring during preimaginal<br />
development. The subdivision <strong>of</strong> the latter into embryonic and larval phases allowed to evidence a possible<br />
selecting effect on embryonic development. The selection would act through the resistance to water loss which is known to<br />
depend on the relative surface <strong>of</strong> the cuticle.In conclusion, our data highlight the importance <strong>of</strong> the emerged period <strong>of</strong> Ae.<br />
aegypti eggs as a critical time for the size <strong>of</strong> future adults, and point to the relative humidity as the likely selecting factor. ©<br />
<strong>2010</strong> Elsevier B.V.<br />
No.818<br />
Author (s) : Moreno, C.; Berg, T.; Tanwandee, T.; Thongsawat, S.; Van V.H.; Zeuzem, S.; Lenz, O.; Peeters, M.; Sekar, V.J.; De S.G.<br />
Title : A PHASE IIA, OPEN-LABEL STUDY TO ASSESS THE ANTIVIRAL ACTIVITY OF TMC435 MONOTHERAPY IN PATIENTS INFECTED WITH<br />
HCV GENOTYPES 2-6<br />
Source : HEPATOLOGY, Volume: 52, Issue: 4, Supplement: S, Pages: 749A-750A, Meeting <strong>Abstract</strong>: 895, Published: OCT <strong>2010</strong><br />
Document Type : Meeting Abstraact<br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.819<br />
Author (s) : Morrison, D., Legg, T.J., Billings, C.W., Forrat, R., Yoksan, S., Lang, J.<br />
Title : A novel tetravalent dengue vaccine is well tolerated and immunogenic against all 4 serotypes in flavivirus-naive adults<br />
Source : Journal <strong>of</strong> Infectious Diseases, Volume 201, Issue 3, 1 February <strong>2010</strong>, Pages 370-377<br />
Document Type : Article<br />
Keywords : dengue vaccine, neutralizing antibody, placebo, sodium chloride, virus antibody; adult, article, asthenia, clinical<br />
trial, controlled clinical trial, controlled study, dengue, Dengue virus, drug fever, drug induced headache, drug safety, drug<br />
tolerability, drug withdrawal, female, human, immune response, immunogenicity, injection site erythema, injection site<br />
pain, injection site swelling, major clinical study, malaise, male, myalgia, nausea, neutrophil count, phase 1 clinical<br />
trial, priority journal, randomized controlled trial, rhinopharyngitis, seroconversion, serotype, side<br />
effect, vaccination, viremia, virus strain, world health organization, blood, classification, drug<br />
administration, immunology, middle aged, serotyping, virology; Adult, Antibodies, Viral, Dengue, Dengue Vaccines, Dengue<br />
Virus, Drug Administration Schedule, Female, Humans, Male, Middle Aged, Serotyping, Viremia, Young Adult<br />
<strong>Abstract</strong> : Background. San<strong>of</strong>i Pasteur has developed a tetravalent dengue vaccine (TDV) against the world's most common arbovirus<br />
infection. Methods. We assessed the safety and immunogenicity <strong>of</strong> the TDV in healthy adults randomized into 2 groups.<br />
Group 1 received 3 TDV injections at months 0, 4, and 12-15; group 2 received saline placebo at month 0 and then 2 TDV<br />
injections at months 4 and 12-15. Adverse events were recorded, and biological parameters and viremia levels were<br />
measured. Neutralizing antibodies against 4 World Health Organization (WHO) reference strains were measured before and<br />
after vaccinations. Results. A total <strong>of</strong> 33 participants were enrolled in each group. Demographic characteristics were<br />
comparable. No vaccine-related serious adverse event was reported. The most common systemic reactions were headache,<br />
malaise, and myalgia. Low viremia levels were detected, mainly <strong>of</strong> serotype 4. Immune response increased with successive<br />
vaccine doses. All participants seroconverted against all 4 serotypes after receiving 3 doses at 0, 4, and 12-15-months, and<br />
almost all seroconverted after 2 doses given 8-11 months apart. Conclusions. San<strong>of</strong>i Pasteur's TDV was well tolerated and<br />
induced full seroconversion against all WHO reference strain serotypes after 3 doses. © <strong>2010</strong> by the Infectious Diseases<br />
Society <strong>of</strong> America. All rights reserved.<br />
No.820<br />
Author (s) : Moukamnerd, C., Kino-Oka, M., Sugiyama, M., Kaneko, Y., Boonchird, C., Harashima, S., Noda, H., Ninomiya, K., Shioya, S.,<br />
Katakura, Y.<br />
Title : Ethanol production from biomass by repetitive solid-state fed-batch fermentation with continuous recovery <strong>of</strong> ethanol<br />
Source : Applied Microbiology and Biotechnology, Volume 88, Issue 1, September <strong>2010</strong>, Pages 87-94<br />
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Document Type : Article<br />
Keywords : Bioethanol; Continuous recovery <strong>of</strong> ethanol; Simultaneous saccharification and fermentation; Solid-state fermentation<br />
<strong>Abstract</strong> : To save cost and input energy for bioethanol production, a consolidated continuous solid-state fermentation system<br />
composed <strong>of</strong> a rotating drum reactor, a humidifier, and a condenser was developed. Biomass, saccharifying enzymes, yeast,<br />
and a minimum amount <strong>of</strong> water are introduced into the system. Ethanol produced by simultaneous saccharification and<br />
fermentation is continuously recovered as vapor from the headspace <strong>of</strong> the reactor, while the humidifier compensates for<br />
the water loss. From raw corn starch as a biomass model, 95∈±∈3, 226∈±∈9, 458∈±∈26, and 509∈±∈64 g∈l-1 <strong>of</strong><br />
ethanol solutions were recovered continuously when the ethanol content in reactor was controlled at 10-20, 30-50, 50-70<br />
and 75-85 g∈kg-mixture-1, respectively. The residue showed a lesser volume and higher solid content than that obtained by<br />
conventional liquid fermentation. The cost and energy for intensive waste water treatment are decreased, and the<br />
continuous fermentation enabled the sustainability <strong>of</strong> enzyme activity and yeast in the system. © <strong>2010</strong> Springer-Verlag.<br />
No.821<br />
Author (s) : Mu, J., Myers, R.A., Jiang, H., Liu, S., Ricklefs, S., Waisberg, M., Chotivanich, K., Wilairatana, P., Krudsood, S., White, N.J.,<br />
Udomsangpetch, R., Cui, L., Ho, M., Ou, F., Li, H., Song, J., Li, G., Wang, X., Seila, S., Sokunthea, S., Socheat, D., Sturdevant, D.E.,<br />
Porcella, S.F., Fairhurst, R.M., Wellems, T.E., Awadalla, P., Su, X.-Z.<br />
Title : Plasmodium falciparum genome-wide scans for positive selection, recombination hot spots and resistance to antimalarial<br />
drugs<br />
Source : Nature Genetics, Volume 42, Issue 3, March <strong>2010</strong>, Pages 268-271<br />
Document Type : Article<br />
Keywords : amodiaquine, antimalarial agent, chloroquine, dihydroartemisinin, fansidar, mefloquine, piperaquine, quinine; antimalarial drug<br />
resistance, article, genetic recombination, genetic selection, genetic trait, IC 50, molecular probe, nonhuman, nucleotide<br />
sequence, pharmacogenetics, Plasmodium falciparum, population structure, priority journal, single nucleotide polymorphism;<br />
Antimalarials, Chromosome Mapping, Cluster Analysis, Comparative Genomic Hybridization, DNA, Protozoan, Drug<br />
Resistance, Genetic Loci, Genome-Wide Association Study, Geography, Inhibitory Concentration 50, Oligonucleotide Array<br />
Sequence Analysis, Plasmodium falciparum, Recombination, Genetic, Selection, Genetic; Plasmodium falciparum<br />
<strong>Abstract</strong> : Antimalarial drugs impose strong selective pressure on Plasmodium falciparum parasites and leave signatures <strong>of</strong> selection in<br />
the parasite genome; screening for genes under selection may suggest potential drug or immune targets. Genome-wide<br />
association studies (GWAS) <strong>of</strong> parasite traits have been hampered by the lack <strong>of</strong> high-throughput genotyping methods,<br />
inadequate knowledge <strong>of</strong> parasite population history and time-consuming adaptations <strong>of</strong> parasites to in vitro culture. Here we<br />
report the first Plasmodium GWAS, which included 189 culture-adapted P. falciparum parasites genotyped using a custombuilt<br />
Affymetrix molecular inversion probe 3K malaria panel array with a coverage <strong>of</strong> 1 SNP per 7 kb. Population structure,<br />
variation in recombination rate and loci under recent positive selection were detected. Parasite half-maximum inhibitory<br />
concentrations for seven antimalarial drugs were obtained and used in GWAS to identify genes associated with drug<br />
responses. This study provides valuable tools and insight into the P. falciparum genome. © <strong>2010</strong> Nature America, Inc. All<br />
rights reserved.<br />
No.822<br />
Author (s) : Muangman, P., Pundee, C., Opasanon, S., Muangman, S.<br />
Title : A prospective, randomized trial <strong>of</strong> silver containing hydr<strong>of</strong>iber dressing versus 1% silver sulfadiazine for the treatment <strong>of</strong><br />
partial thickness burns<br />
Source : <strong>International</strong> Wound Journal, <strong>2010</strong>, 7 (4), pp. 271 – 276.<br />
Document Type : Article<br />
Keywords : Burns; Dressing; Hydr<strong>of</strong>iber; Silver sulfadiazine<br />
<strong>Abstract</strong> : Silver sulfadiazine has been used as a topical burn wound treatment for many years. Pain associated with dressing changes is<br />
a common problem in burn wounds. Aquacel Ag, a hydr<strong>of</strong>iber dressing coated with ionic silver has been reported to reduce<br />
burn wound infection and promote antimicrobial activity. The purpose <strong>of</strong> this study was to show the benefits <strong>of</strong> Aquacel Ag<br />
for the treatment <strong>of</strong> partial thickness burns. This prospective randomized study was conducted in 70 patients who had partial<br />
thickness burns less than 15% <strong>of</strong> total body surface area and were treated at Siriraj outpatient burn clinic during December<br />
2006-February 2008. Patients were divided into two groups: Aquacel Ag-treated group with dressing changes every 3 days (35<br />
patients) and 1% silver sulfadiazine-treated group, with daily dressing changes (35 patients). There was no difference in<br />
demographic data including age, gender, burn percentage between groups. Time-to-wound healing pain score during dressing<br />
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change and cost <strong>of</strong> treatment were compared between both groups. Time-to-wound closure was significantly shorter in the<br />
Aquacel Ag-treated group (10 ± 3 versus 13.7 ± 4 days, P < 0·02) as well as pain scores at days 1, 3 and 7 (4·1 ± 2·1, 2·1 ± 1·8,<br />
0·9 ± 1·4 versus 6·1 ± 2·3, 5·2 ± 2·1, 3·3 ± 1·9, respectively, P < 0·02). Total cost <strong>of</strong> treatment was 52 ± 29 US dollars for the<br />
Aquacel Ag-treated group versus 93 ± 36 US dollars for the silver sulfadiazine-treated group. This study showed that Aquacel<br />
Ag increased time to healing, decreased pain symptoms and increased patient convenience because <strong>of</strong> limiting the frequency<br />
<strong>of</strong> replacement <strong>of</strong> the dressing at lower total cost. This study confirms the efficacy <strong>of</strong> Aquacel Ag for the treatment <strong>of</strong> partial<br />
thickness burns at an outpatient clinic. © <strong>2010</strong> Blackwell Publishing Ltd and Medicalhelplines.com Inc.<br />
No.823<br />
Author (s) : Muangpaisan, W., Brayne, C.<br />
Title : Systematic review <strong>of</strong> statins for the prevention <strong>of</strong> vascular dementia or dementia<br />
Source : Geriatrics and Gerontology <strong>International</strong>, Volume 10, Issue 2, April <strong>2010</strong>, Pages 199-208<br />
Document Type : Article<br />
Keywords : Dementia; Prevention; Statins; Vascular dementia<br />
<strong>Abstract</strong> : Aim: Non-hypolipidemic effects <strong>of</strong> statins, known as pleiotropic effects, are likely to explain the effect <strong>of</strong> statins on dementia.<br />
Results <strong>of</strong> the relationship between statins and dementia in previous studies are conflicting. There is no systematic review<br />
investigating the effect <strong>of</strong> statins on vascular dementia (VaD). This systematic review evaluates the role <strong>of</strong> statins in the<br />
prevention <strong>of</strong> VaD or dementia. The possible causes <strong>of</strong> conflicting results in the existing published work will be explored.<br />
Methods: Relevant studies were systematically identified and reviewed. The Cochrane Controlled Trials and three electronic<br />
databases (MEDLINE, EMBASE and PsycInfo) were searched. The selection criteria were defined a priori. Included studies were<br />
rated by quality assessment checklists and two independent reviewers. Results: Six studies in dementia, two studies in VaD<br />
(one study reported both dementia and VaD) and two meta-analyses met the selection criteria. The studies covered 1372<br />
cases <strong>of</strong> dementia from 14 430 participants and 116 cases <strong>of</strong> VaD from 4924 participants from the USA and UK. There was no<br />
association between statin use and risk <strong>of</strong> VaD. The protective effect <strong>of</strong> statins on dementia was demonstrated only in a<br />
nested case-control study <strong>of</strong> lower quality and one recently published cohort study. In most other cohort and high quality<br />
studies, statin use did not show a beneficial effect. Conclusion: Study design differences among the studies and<br />
methodological shortcomings may have resulted in different outcomes. On the basis <strong>of</strong> these conflicting results, statins could<br />
not be recommended as a preventative treatment for dementia. © <strong>2010</strong> Japan Geriatrics Society.<br />
No.824<br />
Author (s) : Muangpaisan, W., Intalapaporn, S., Assantachai, P.<br />
Title : Digit span and verbal fluency tests in patients with mild cognitive impairment and normal subjects in Thai-community<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 2, February <strong>2010</strong>, Pages 224-230<br />
Document Type : Conference Paper<br />
Keywords : Community; Digit span; Elderly; Mild cognitive impairment; Verbal fluency<br />
<strong>Abstract</strong> : Background: Far too little attention has been paid to the difference <strong>of</strong> Digit Span test and category verbal fluency test (CVFT)<br />
between normal and mild cognitive impairment (MCI) subjects. Objective: To investigate the difference <strong>of</strong> Digit Span test and<br />
CVFT between normal subjects and patients with MCI and study the influence <strong>of</strong> age, gender, and education on the task<br />
performance. Material and Method: The authors collected data <strong>of</strong> 77 participants diagnosed with amnestic MCI (from 517<br />
participants screened) and 30 normal subjects aged 50 or over enrolled from communities in Bangkok. The Digit Span test and<br />
CVFT (semantic fluency and Controlled word association test for letter fluency) were used to evaluate the subjects. Results:<br />
MCI patients had significantly lower digit span score, in both Digits Forward and Digits Backward, poorer performance on<br />
semantic fluency for animals and fruits and letter fluency test. The logistic regression model <strong>of</strong> MCI diagnosis showed that<br />
only Digits Backward score was a predictor <strong>of</strong> MCI diagnosis (OR 0.643 for each increment <strong>of</strong> 1 digit, p = 0.009, 95% confidence<br />
interval 0.462-0.896). The cut-<strong>of</strong>f point <strong>of</strong> Digit Backward score was 4 and yielded sensitivity <strong>of</strong> 77% and specificity <strong>of</strong> 57%.<br />
Females had lower scores than males in every test except semantic fluency for fruits. The digit span and semantic fluency<br />
scores decreased as age increased but letter fluency increased correspondently with age. The digit span and CVFT scores<br />
increased in parallel with the increase <strong>of</strong> education. Conclusion: MCI patients had poorer performance on the Digit Span and<br />
CVFT tests than normal age and education matched subjects. Digits Backward test can predict the MCI diagnosis. Age, gender<br />
and education have an impact on the performance <strong>of</strong> the tests.<br />
304
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.825<br />
Author (s) : Muangpaisan, W., Praditsuwan, R., Assanasen, J., Srinonprasert, V., Assantachai, P., Intalapaporn, S., Chatthanawaree, W.,<br />
Dajpratham, P., Kuptniratsaikul, V., Pisansalakij, D.<br />
Title : Caregiver burden and needs <strong>of</strong> dementia caregivers in Thailand: A cross-sectional study<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 5, May <strong>2010</strong>, Pages 601-607<br />
Document Type : Article<br />
Keywords : Caregiver burden; Dementia<br />
<strong>Abstract</strong> : Objective: To identify the burdens <strong>of</strong> Thai dementia caregivers and to determine the services that could support them in this<br />
function. Material and Method: The authors surveyed 88 dementia caregivers attending "Caregiver Day". The questionnaire<br />
contained Caregiver Burden Inventory. The answers range from "not at all descriptive" (zero) to "very descriptive" (4). The<br />
authors also explored baseline characteristics <strong>of</strong> caregivers and care recipients as well as caregiver's needs <strong>of</strong> a supporting<br />
system. Results: There was an 82% response rate. Responses in time-dependence burden distributed almost equally in the<br />
five possible scales. In developmental and physical burden, caregivers rate scores mainly from 0-2. The scores in social and<br />
emotional burden ranged mainly between 0-1. Dependency in basic activities <strong>of</strong> daily living correlated with higher caregiver<br />
burden (odd ratio 7.48, 95% confidence interval 1.42-39.53, p = 0.02), while sex and kinship did not. The top three caregiver's<br />
needs were 1) caregiver education and training, 2) telephone line provided for caregiver consultation and 3) special system in<br />
a hospital provided for dementia patients to have rapid access to see a doctor. Conclusion: Caring for dementia patients can<br />
lead to high caregiver burden, particularly those caring for dependent patients. Physical and developmental burdens are<br />
affected more than social and emotional burdens. Culture, relationship quality and resources (coping, outlook on life and<br />
social support) might be contributing factors <strong>of</strong> this difference.<br />
No.826<br />
Author (s) : Mungnirandr, A., Khorana, J., Ruangtrakool, R.<br />
Title : Correlation between duration <strong>of</strong> postoperative parenteral nutrition and incidence <strong>of</strong> postoperative complication in<br />
gastroschisis patients<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 4, April <strong>2010</strong>, Pages 443-448<br />
Document Type : Review<br />
Keywords : Gastroschisis; Necrotizing enterocolitis; Post operative complications; Total parenteral nutrition<br />
<strong>Abstract</strong> : Background: Gastroschisis is the most common neonatal anterior abdominal wall defect. Since all neonatal infants with<br />
gastroschisis require total parenteral nutrition (TPN), the authors retrospectively review was to find correlation between<br />
duration <strong>of</strong> TPN and postoperative complications including necrotizing enterocolitis (NEC) and poor feeding in gastroschisis<br />
patients. The secondary objective <strong>of</strong> the present study was to find correlation(s) among length <strong>of</strong> stay (LOS), gestational age<br />
(GA), birthweight(BW), type <strong>of</strong> operation and postoperative complications. Material and Method: Forty-one gastroschisis<br />
patients were reviewed during 2001 to 2006. Postoperative complications were analysed among duration <strong>of</strong> TPN, LOS, GA,<br />
BW, and type <strong>of</strong> operation. Multiple parameters affecting gastroschisis patients with NEC were compared with those without<br />
NEC. Results: There were 21 male and 20 female infants. Normal labour was the major route <strong>of</strong> delivery (31 in 39 infants).<br />
Complications from TPN usage included line infection 5/40, central line requirement 9/40, cutdown requirement 5/40, sepsis<br />
7/40, and TPN leakage 1/40. NEC and poor feeding were 6/40 and 9/40 respectively. In the present study, the Incidence <strong>of</strong><br />
NEC in gastroschisis was 15%. NEC and poor feeding were significantly correlated with longer TPN administration (p = 0.0046 &<br />
p = 0.0042). Lowest bodyweight <strong>of</strong> gastroschisis infants occured in the 5.39 + 3.19 days after birth. Gastroschisis patients with<br />
NEC were frequently found with low birthweight and premature (p = 0.007 & 0.028). All gastroschisis infants with NEC were<br />
delivered vaginally. No correlation was shown between time to first oral feeding and development <strong>of</strong> NEC (p = 0.964).<br />
Conclusion: Longer TPN administration time was found in gastroschisis patients with NEC and poor feeding. Duration <strong>of</strong> NPO<br />
after operation did not associate with NEC development. NEC was more likely to occur in gastroschisis patients with low<br />
birthweight and prematurity. All <strong>of</strong> the gastroschisis infants with NEC were born through the vaginal route. Like other newborn<br />
babies, gastroschisis patients lose their bodyweight during the first week <strong>of</strong> life and gradually gain bodyweight thereafter.<br />
No.827<br />
Author (s) : Munkongdee, T., Vattanaviboon, P., Thummarati, P., Sewamart, P., Winichagoon, P., Fucharoen, S., Svasti, S.<br />
Title : Rapid diagnosis <strong>of</strong> α-thalassemia by melting curve analysis<br />
Source : Journal <strong>of</strong> Molecular Diagnostics, Volume 12, Issue 3, 1 May <strong>2010</strong>, Pages 354-358<br />
Document Type : Article<br />
305
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : alpha globin, hemoglobin; alpha thalassemia, article, controlled study, diagnostic accuracy, gene amplification, gene<br />
deletion, heterozygosity, homozygosity, human, major clinical study, multiplex polymerase chain reaction, temperature<br />
sensitivity; alpha-Thalassemia, Genotype, Hemoglobins, Humans, Nucleic Acid Denaturation, Polymerase Chain Reaction<br />
<strong>Abstract</strong> : α-Thalassemia is an inherited hemoglobin disorder that results from defective synthesis <strong>of</strong> α-globin protein. Couples who<br />
both carry the α-thalassemia-1 gene are at risk <strong>of</strong> having a fetus with Hb Bart's hydrops fetalis. Rapid and accurate screening<br />
for individuals carrying the α-thalassemia-1 gene is the most effective strategy to prevent and control this severe form <strong>of</strong><br />
thalassemia. In this study, a new and accurate method for α-thalassemia diagnosis was developed by genotyping αthalassemia-1,<br />
the Southeast Asian type (--SEA) and Thai type (--THAI) deletions, using multiplex PCR followed by a melting<br />
curve analysis. Primers were designed to specifically amplify two deletion fragments, the -- SEA and --THAI deletions and two<br />
normal fragments, ψζ- and α2-globin gene. The primers were capable <strong>of</strong> distinguishing α-thalassemia 1 heterozygotes from<br />
α-thalassemia 2 homozygotes, which are unable to be diagnosed by standard hematological data and hemoglobin typing.<br />
The melting temperatures <strong>of</strong> the --THAI, -- SEA, ψζ-globin, and α2-globin gene fragments were 79.9 ± 0.2, 89.4 ± 0.5, 92.8 ±<br />
0.2, and 85.0 ± 0.2°C, respectively. Melting curve analysis was performed in 130 subjects in parallel with conventional gap-PCR<br />
analysis, and results showed 100% concordance. This method eliminates the post-PCR electrophoresis process, which is<br />
laborious, and allows high throughput screening suitable for large population screening for prevention and control <strong>of</strong><br />
thalassemia. Copyright © American Society for Investigative Pathology and the Association for Molecular Pathology.<br />
No.828<br />
Author (s) : Mwesigwa, J., Parikh, S., McGee, B., German, P., Drysdale, T., Kalyango, J.N., Clark, T.D., Dorsey, G., Lindegardh, N., Annerberg, A.,<br />
Rosenthal, P.J., Kamya, M.R., Aweeka, F.<br />
Title : Pharmacokinetics <strong>of</strong> artemether-lumefantrine and artesunate-amodiaquine in children in Kampala, Uganda<br />
Source : Antimicrobial Agents and Chemotherapy, Volume 54, Issue 1, January <strong>2010</strong>, Pages 52-59<br />
Document Type : Article<br />
Keywords : amodiaquine, artemether plus benflumetol, artesunate, deethylamodiaquine, dihydroartemisinin; adolescent, area under the<br />
curve, article, child, clinical trial, controlled clinical trial, controlled study, dose response, drug elimination, drug half life, drug<br />
safety, human, major clinical study, malaria, maximum plasma concentration, preschool child, priority journal, randomized<br />
controlled trial, Uganda, vomiting; Amodiaquine, Antimalarials, Area Under Curve, Artemisinins, Biotransformation, Body<br />
Weight, Child, Child, Preschool, Chromatography, Liquid, Drug Combinations, Ethanolamines, Female, Fluorenes, Half-<br />
Life, Humans, Longitudinal Studies, Male, Spectrophotometry, Ultraviolet, Uganda<br />
<strong>Abstract</strong> : The World Health Organization recommends the use <strong>of</strong> artemisinin-based combination therapies (ACTs) for the treatment <strong>of</strong><br />
uncomplicated malaria. The two most widely adopted ACT regimens are artemether (AR)-lumefantrine (LR) (the combination<br />
is abbreviated AL) and amodiaquine (AQ)-artesunate (AS). Pharmacokinetic (PK) data informing the optimum dosing <strong>of</strong> these<br />
drug regimens is limited, especially in children. We evaluated PK parameters in Ugandan children aged 5 to 13 years with<br />
uncomplicated malaria treated with AL (n = 20) or AQ-AS (n = 21), with intensive venous sampling occurring at 0, 2, 4, 8, 24,<br />
and 120 h following administration <strong>of</strong> the last dose <strong>of</strong> 3-day regimens <strong>of</strong> AL (twice daily) or AQ-AS (once daily). AS achieved<br />
an estimated maximum concentration in plasma (Cmax) <strong>of</strong> 51 ng/ml and an area under the concentration-time curve from<br />
time zero to infinity (AUC0-∞) <strong>of</strong> 113 ng · h/ml; and its active metabolite, dihydroartemisinin (DHA), achieved a geometric<br />
mean Cmax <strong>of</strong> 473 ng/ml and an AUC0-∞ <strong>of</strong> 1,404 ng · h/ml. AR-DHA exhibited a Cmax <strong>of</strong> 34/119 ng/ml and an AUC 0-∞ <strong>of</strong><br />
168/382 ng · h/ml, respectively. For LR, Cmax and AUC0-∞ were 6,757 ng/ml and 210 μg · h/ml, respectively. For AQ and its<br />
active metabolite, desethylamodiaquine (DEAQ), the Cmaxs were 5.2 ng/ml and 235 ng/ml, respectively, and the AUC 0-∞s<br />
were 39.3 ng · h/ml and 148 μg · h/ml, respectively. Comparison <strong>of</strong> the findings <strong>of</strong> the present study to previously published<br />
data for adults suggests that the level <strong>of</strong> exposure to LR is lower in children than in adults and that the level <strong>of</strong> AQ-DEAQ<br />
exposure is similar in children and adults. For the artemisinin derivatives, differences between children and adults were<br />
variable and drug specific. The PK results generated for children must be considered to optimize the dosing strategies for<br />
these widely utilized ACT regimens. Copyright © <strong>2010</strong>, American Society for Microbiology. All Rights Reserved.<br />
No.829<br />
Author (s) : Myint, H.Y., Ohrt, C., Walker, L., White, N., Magill, A.<br />
Title : A SURVEY ON CLINICAL SAFETY OF 8-AMINOQUINOLINES<br />
Source : AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, Volume: 83, Issue: 5, Supplement: S, Pages: 371-372, Meeting<br />
<strong>Abstract</strong>: 1249, Published: NOV <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
306
Keywords : –<br />
<strong>Abstract</strong> : –<br />
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.830<br />
Author (s) : Naenna, P., Noisumdaeng, P., Pongpech, P., Tribuddharat, C.<br />
Title : Detection <strong>of</strong> outer membrane porin protein, an imipenem influx channel, in Pseudomonas aeruginosa clinical isolates<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 3, May <strong>2010</strong>, Pages 614-624<br />
Document Type : Article<br />
Keywords : Carbapenem resistance; OprD porin protein; Pseudomonas aeruginosa<br />
<strong>Abstract</strong> : Decreased permeability to imipenem is the most frequent mechanism <strong>of</strong> imipenem resistance in Pseudomonas aeruginosa.<br />
We have determined the presence <strong>of</strong> OprD porin protein, an imipenem influx channel, in 70 carbapenem-resistant P.<br />
aeruginosa clinical isolates by Western blot analysis using rabbit anti-OprD polyclonal antibody. Ninety-eight percent (54 <strong>of</strong> 55<br />
isolates) <strong>of</strong> imipenem-and meropenem-resistant P. aeruginosa clinical isolates were negative for OprD porin production. A<br />
small group <strong>of</strong> isolates resistant to imipenem but susceptible to meropenem (2 isolates) produced OprD protein but at a<br />
level 3-5 times lower than the wild type P. aeruginosa ATCC27853 strains. This study indicates that the loss <strong>of</strong> OprD porin<br />
protein was the main mechanism for imipenem resistance in P. aeruginosa clinical isolates. Determination <strong>of</strong> the status <strong>of</strong><br />
OprD level in P. aeruginosa may help in the better selection <strong>of</strong> appropriate carbapenem antibiotics.<br />
No.831<br />
Author (s) : Nair, S., Nkhoma, S., Nosten, F., Mayxay, M., French, N., Whitworth, J., Anderson, T.<br />
Title : Genetic changes during laboratory propagation: Copy number At the reticulocyte-binding protein 1 locus <strong>of</strong> Plasmodium<br />
falciparum<br />
Source : Molecular and Biochemical Parasitology, Volume 172, Issue 2, August <strong>2010</strong>, Pages 145-148<br />
Document Type : Article<br />
Keywords : Cell invasion; FCR3; Growth rate; Malaria; Microsatellite; Structural variation<br />
<strong>Abstract</strong> : Comparative genomic hybridization studies have revealed elevated copy number (CN) at the reticulocyte-binding protein 1<br />
gene (PfRh1) in fast growing lab-adapted parasites, while genetic manipulation demonstrates a causal link between cell<br />
invasion and PfRh1 CN. We therefore examined PfRh1 copy number variation (CNV) in 202 single clone parasite isolates from<br />
four countries to quantify the extent <strong>of</strong> CNV within natural populations. Surprisingly, we found that no natural parasite<br />
infections showed elevated CN. In contrast, 4/28 independent laboratory reference strains show elevated CN. One possibility<br />
is that amplification <strong>of</strong> PfRh1 (or neighboring loci) is selected during laboratory culture. In the case <strong>of</strong> FCR3 group <strong>of</strong> parasites,<br />
clone trees show that PfRh1 amplification arose in laboratory lines following establishment in culture. These data show that<br />
CNV at PfRh1 is rare or non-existent in natural populations, but can arise during laboratory propagation. We conclude that<br />
PfRh1 CNV is not an important determinant <strong>of</strong> gene expression, cell invasion or growth rate in natural parasite populations. ©<br />
<strong>2010</strong> Elsevier B.V.<br />
No.832<br />
Author (s) : Nakamura, K., Schwartz, B.S., Lindegårdh, N., Keh, C., Guglielmo, B.J.<br />
Title : Possible neuropsychiatric reaction to high-dose oseltamivir during acute 2009 H1N1 influenza a infection<br />
Source : Clinical Infectious Diseases, Volume 50, Issue 7, 1 April <strong>2010</strong>, Pages e47-e49<br />
Document Type : Article<br />
<strong>Abstract</strong> : The safety <strong>of</strong> high-dose oseltamivir during treatment <strong>of</strong> 2009 H1N1 influenza A infection for critically ill patients is unknown.<br />
Here we report on a case patient with severe, delayed-onset neuropsychiatric symptoms after administration <strong>of</strong> high-dose<br />
oseltamivir. Clinicians should be vigilant to the possible increased risk <strong>of</strong> complications associated with high-dose oseltamivir<br />
therapy for 2009 H1N1 influenza A infection. © <strong>2010</strong> by the Infectious Diseases Society <strong>of</strong> America. All rights reserved.<br />
Keywords : 4 acetamido 5 amino 3 (1 ethylpropoxy) 1 cyclohexene 1 carboxylic acid, aciclovir, cefepime, docusate<br />
sodium, electrolyte, entecavir, heparin, oseltamivir, pantoprazole, recombinant granulocyte colony stimulating factor;<br />
adult, arterial gas, article, autologous stem cell transplantation, case report, computer assisted<br />
tomography, coughing, critically ill patient, drug blood level, drug dose escalation, drug efficacy, drug<br />
safety, fever, human, hypoxia, influenza, Influenza virus A H1N1, male, mental<br />
health, neuropsychiatry, neurotoxicity, neutropenia, nonhuman, pneumonia, polymerase chain reaction, priority journal, risk<br />
factor, Senna, sinus congestion, sinusitis, sphenoid sinus, thrombocytopenia, treatment response, visual hallucination;<br />
307
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Adult, Antiviral Agents, Confusion, Hallucinations, Humans, Influenza A Virus, H1N1 Subtype, Influenza, Human, Leukemia,<br />
Promyelocytic, Acute, Male, Neurotoxicity Syndromes, Oseltamivir, Transplantation, Autologous<br />
No.833<br />
Author (s) : Nakano, K., Nomura, R., Taniguchi, N., Lapirattanakul, J., Kojima, A., Naka, S., Senawongse, P., Srisatjaluk, R., Grönroos, L.,<br />
Alaluusua, S., Matsumoto, M., Ooshima, T.<br />
Title : Molecular characterization <strong>of</strong> Streptococcus mutans strains containing the cnm gene encoding a collagen-binding adhesion<br />
Source : Archives <strong>of</strong> Oral Biology, Volume 55, Issue 1, January <strong>2010</strong>, Pages 34-39<br />
Document Type : Article<br />
Keywords : Clinical isolates; Collagen-binding adhesin; Glucan-binding activity; Streptococcus mutans<br />
<strong>Abstract</strong> : Objective: Streptococcus mutans, known to be a major pathogen <strong>of</strong> dental caries, is also considered to cause infective<br />
endocarditis. Its 120-kDa Cnm protein binds to type I collagen, which may be a potential virulence factor. In this study, we<br />
characterized S. mutans clinical strains focusing on the cnm gene encoding Cnm. Design: A total <strong>of</strong> 528 S. mutans strains<br />
isolated from Japanese, Finnish, and Thai subjects were investigated. Using molecular techniques, the distribution frequency<br />
<strong>of</strong> cnm-positive strains and location <strong>of</strong> the inserted cnm were analyzed. Furthermore, isogenic mutant strains were<br />
constructed by inactivation <strong>of</strong> the cnm gene, then their biological properties <strong>of</strong> collagen-binding and glucan-binding were<br />
evaluated. Southern hybridization <strong>of</strong> the genes encoding glucan-binding proteins was also performed. Results: The distribution<br />
frequency <strong>of</strong> cnm-positive strains from Thai subjects was 12%, similar to that previously reported for Japanese and Finnish<br />
subjects. Furthermore, the location <strong>of</strong> insertion <strong>of</strong> cnm was the same in all cnm-positive clinical isolates. As for the cnminactivated<br />
mutant strains constructed from 28 clinical isolates, their collagen-binding activity was negligible. In addition,<br />
glucan-binding activity in the cnm-positive clinical isolates was significantly reduced and corresponded to a lack <strong>of</strong> gbpA<br />
encoding glucan-binding protein A. Conclusions: Our results indicate that strains with cnm genes, the most crucial factor for<br />
the collagen-binding property <strong>of</strong> S. mutans, are detectable at similar frequencies over several different geographic locations.<br />
In addition, the common properties <strong>of</strong> these strains are a high level <strong>of</strong> collagen-binding activity and tendency for a low level<br />
<strong>of</strong> glucan-binding activity. © 2009 Elsevier Ltd. All rights reserved.<br />
No.834<br />
Author (s) : Nakkrasae, L.-I., Thongon, N., Thongbunchoo, J., Krishnamra, N., Charoenphandhu, N.<br />
Title : Transepithelial calcium transport in prolactin-exposed intestine-like Caco-2 monolayer after combinatorial knockdown <strong>of</strong><br />
TRPV5, TRPV6 and Ca v1.3<br />
Source : Journal <strong>of</strong> Physiological Sciences, Volume 60, Issue 1, January <strong>2010</strong>, Pages 9-17<br />
Document Type : Article<br />
Keywords : Calcium absorption; Small interfering RNA (siRNA); Transcellular transport; Triple knockdown; Voltage-dependent calcium<br />
channel (Cav)<br />
<strong>Abstract</strong> : The milk-producing hormone prolactin (PRL) increases the transcellular intestinal calcium absorption by enhancing apical<br />
calcium uptake through voltage-dependent L-type calcium channel (Cav) 1.3. However, the redundancy <strong>of</strong> apical calcium<br />
channels raised the possibility that Ca v1.3 may operate with other channels, especially transient receptor potential vanilloid<br />
family calcium channels (TRPV) 5 or 6, in an interdependent manner. Herein, TRPV5 knockdown (KD), TRPV5/TRPV6,<br />
TRPV5/Cav1.3, and TRPV6/Cav1.3 double KD, and TRPV5/TRPV6/Cav1.3 triple KD Caco-2 monolayers were generated by<br />
transfecting cells with small interfering RNAs (siRNA). siRNAs downregulated only the target mRNAs, and did not induce<br />
compensatory upregulation <strong>of</strong> the remaining channels. After exposure to 600 ng/mL PRL, the transcellular calcium transport<br />
was increased by ~2-fold in scrambled siRNA-treated, TRPV5 KD and TRPV5/TRPV6 KD monolayers, but not in TRPV5/Ca v1.3,<br />
TRPV6/Cav1.3 and TRPV5/TRPV6/Cav1.3 KD monolayers. The results suggested that Cav1.3 was the sole apical channel<br />
responsible for the PRL-stimulated transcellular calcium transport in intestine-like Caco-2 monolayer. © 2009 The<br />
Physiological Society <strong>of</strong> Japan and Springer.<br />
No.835<br />
Author (s) : Nakkrasae, S., Kraipeerapun, P., Amornsamankul, S., Fung, C.C.<br />
Title : Bagging <strong>of</strong> complementary neural networks with double dynamic weight averaging<br />
Source : Proceedings - 11th ACIS <strong>International</strong> Conference on S<strong>of</strong>tware Engineering, Artificial Intelligence, Networking and<br />
Parallel/Distributed Computing, SNPD<strong>2010</strong>, <strong>2010</strong>, Article number 5521520, Pages 173-178<br />
Document Type : Conference Paper<br />
308
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : Backpropagation neural network; Bagging; Complementary Neural Networks; Diversity; Ensemble<br />
<strong>Abstract</strong> : Ensemble technique has been widely applied in regression problems. This paper proposes a novel approach <strong>of</strong> the ensemble<br />
<strong>of</strong> Complementary Neural Network (CMTNN) using double dynamic weight averaging. In order to enhance the diversity in the<br />
ensemble, different training datasets created based on bagging technique are applied to an ensemble <strong>of</strong> pairs <strong>of</strong> feed-forward<br />
back-propagation neural networks created to predict the level <strong>of</strong> truth and falsity values. In order to obtain more accuracy,<br />
uncertainties in the prediction <strong>of</strong> truth and falsity values are used to weight the prediction results in two steps. In the first<br />
step, the weight is used to average the truth and the falsity values whereas the weight in the second step is used to calculate<br />
the final regression output. The proposed approach has been tested with benchmarking UCI data sets. The results derived<br />
from our technique improve the prediction performance while compared to the traditional ensemble <strong>of</strong> neural networks<br />
which is predicted based on only the truth values. Furthermore, the obtained results from our novel approach outperform<br />
the results from the existing ensemble <strong>of</strong> complementary neural network. © <strong>2010</strong> IEEE.<br />
No.836<br />
Author (s) : Nakornchai, S., Banditsing, P., Visetratana, N.<br />
Title : Clinical evaluation <strong>of</strong> 3Mix and Vitapex® as treatment options for pulpally involved primary molars<br />
Source : <strong>International</strong> Journal <strong>of</strong> Paediatric Dentistry, Volume 20, Issue 3, May <strong>2010</strong>, Pages 214-221<br />
Document Type : Article<br />
Keywords : 3Mix ointment, antiinfective agent, calcium hydroxide, cipr<strong>of</strong>loxacin, macrogol derivative, metronidazole, minocycline,<br />
propylene glycol, root canal filling material, silicone derivative, Vitapex; article, chemistry, child, clinical trial, controlled<br />
clinical trial, controlled study, deciduous tooth, drug combination, drug effect, endodontics, follow up, human, methodology,<br />
molar tooth, ointment, preschool child, prospective study, radiography, randomized controlled trial, single blind procedure,<br />
tooth pulp disease, treatment outcome; Anti-Bacterial Agents, Calcium Hydroxide, Child, Child, Preschool, Cipr<strong>of</strong>loxacin,<br />
Dental Pulp Necrosis, Drug Combinations, Follow-Up Studies, Humans, Metronidazole, Minocycline, Molar, Ointments,<br />
Polyethylene Glycols, Propylene Glycol, Prospective Studies, Root Canal Filling Materials, Root Canal Therapy, Silicones,<br />
Single-Blind Method, Tooth, Deciduous, Tooth, Nonvital, Treatment Outcome<br />
<strong>Abstract</strong> : The aim <strong>of</strong> this study was to compare the clinical and radiographic success <strong>of</strong> 3Mix and Vitapex® for root canal treatment <strong>of</strong><br />
pulpally involved primary molars. Methods.: Fifty teeth from 37 healthy children aged 3-8 years with pulpally involved<br />
primary molars needing root canal procedures were treated with 3Mix or Vitapex® before restoration with stainless steel<br />
crowns. The research employed a prospective single-blinded randomized design. The subjects were followed up clinically<br />
and radiographically at 6 and 12 months, respectively. The outcome was compared using a Z-test with a significance level <strong>of</strong><br />
0.05. Results.: Both groups showed 100% and 96% clinical success at 6 and 12 months, respectively. At 6 months,<br />
radiographic success <strong>of</strong> 3Mix and Vitapex® was 84% and 80%, respectively, and at 12 months, radiographic success <strong>of</strong> 3Mix<br />
and Vitapex® was 76% and 56%, respectively. Considering the radiographic findings at the end <strong>of</strong> 6 and 12 months, no<br />
statistically significant differences were found between the two groups (P = 0.356 and 0.068, respectively). Conclusion.: 3Mix<br />
and Vitapex® can be used as a root canal treatment agent in pulpally involved primary teeth. © <strong>2010</strong> The Authors. Journal<br />
compilation © <strong>2010</strong> BSPD, IAPD and Blackwell Publishing Ltd.<br />
No.837<br />
Author (s) : Nakponetong, K.; Srisawat, C.; Benjasupattananun, P.; Suratannon, C.; Wachairutmanggur, L.; Boonchoo, S.; Pankaew, D.;<br />
Laochareonkait, A.; Pacharn, P.; Jirapongsananuruk, O.; Visitsunthorn, N.; Vichyanond, P.<br />
Title : Pharmacokinetics Of Epinephrine Absorption via Intranasal Administration: A Preliminary Report<br />
Source : JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, Volume: 125, Issue: 2, Supplement: 1, Pages: AB219-AB219, Published:<br />
FEB <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.838<br />
Author (s) : Nakwan, N., Nakwan, N., Chokephaibulkit, K.<br />
Title : Pasteurella multocida infection in the neonates<br />
Source : Pediatric Infectious Disease Journal, Volume 29, Issue 2, February <strong>2010</strong>, Page 192<br />
Document Type : Letter<br />
309
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : ampicillin; bacterium culture, drug megadose, human, infection risk, letter, newborn, newborn<br />
mortality, nonhuman, Pasteurella multocida, pasteurellosis, priority journal, vertical transmission; Animals, Humans, Infant,<br />
Newborn, Pasteurella Infections, Pasteurella multocida, Zoonoses<br />
<strong>Abstract</strong> : –<br />
No.839<br />
Author (s) : Nakwanit S., Visoottiviseth P., Khokiattiwong S., Sangchoom W.<br />
Title : Management <strong>of</strong> arsenic-accumulated waste from constructed wetland treatment <strong>of</strong> mountain tap-water<br />
Source : Journal <strong>of</strong> Hazardous Materials, Volume 185, Issue 2-3, 30 January 2011, Pages 1081-1085<br />
Document Type : Article<br />
Keywords : Arsenic; Constructed wetland plant; Degradation; Leaching test; Solidification/stabilization<br />
<strong>Abstract</strong> : Arsenic-contaminated mountain tap water supply in Ron Phibun District, Nakorn Si Thammarat Province, Thailand poses a<br />
health hazard. Arsenic was removed using a constructed wetland (CW) system, in which the treated contaminated tap water<br />
was sedimented in 3 consecutive ponds before entering into the CW ponds, containing either Cyperus spp. or Colocasia<br />
esculenta. Following 1 year <strong>of</strong> operation both plants had similar ability to remove arsenic from mountain tap water. Arsenic<br />
was mostly concentrated at the roots <strong>of</strong> both plants. Arsenic in C. esculenta leaves was allowed to leach out in freshwater<br />
for 149 days, but the level (0.05mgL-1) was much lower than standard guidelines for industrial discharge. For Cyperus spp.,<br />
young shoots were utilized as ornamental plants. As the sediments contained high arsenic levels, they were converted by a<br />
solidification/stabilization (S/S) system into cement-containing blocks, which after curing for 21 days produced arsenic<br />
leaching at levels that did not require a secure landfill for storage. The success <strong>of</strong> this study demonstrated that CW combined<br />
with appropriate S/S system is a suitable approach for Thailand in removing arsenic from contaminated water. © <strong>2010</strong> Elsevier<br />
B.V.<br />
No.840<br />
Author (s) : Namyen, J.; Buntup, D.; Sitdhiraksa, N.; Prapunpoj, V; Govitrapong, P.<br />
Title : Association <strong>of</strong> the 5-HTTLPR polymorphism in Thai amphetamine users<br />
Source : JOURNAL OF NEUROCHEMISTRY, Volume: 115, Supplement: 1, Pages: 83-83, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.841<br />
Author (s) : Nantakomol, D., Chanprasert, S., Soogarun, S., Imwong, M., Ohashi, J., Nuchnoi, P.<br />
Title : Affordable technology for enumeration <strong>of</strong> the absolute CD4 T-lymphocyte count by cell bead assay<br />
Source : Laboratory Medicine, Volume 41, Issue 7, July <strong>2010</strong>, Pages 423-428<br />
Document Type : Article<br />
Keywords : Absolute count; Cell bead; Flow cytometry; Flow rate based calibration; Trucount<br />
<strong>Abstract</strong> : The quantitative BD Trucount (San Jose, CA) tube method is the conventional but expensive method to quantitate CD4+Tlymphocyte<br />
(CD4) counts, and this may be beyond the means <strong>of</strong> countries with limited resources. In this study, we validated<br />
a quantitative method known as a cell-bead (CB) assay to quantitate CD4 counts in the peripheral blood <strong>of</strong> healthy subjects.<br />
The absolute CD4 count obtained from the CB method was highly correlated with those obtained from the Trucount tube<br />
(r2=0.98, y=26.73+1.01x, P<0.0001 and a mean bias <strong>of</strong> 34.8 cell/μL, limit <strong>of</strong> agreement [LOA] -34.8-104.4 cell/μL) and flow<br />
rate-based assay method (r2=0.97; y=69.51 + 0.88x, P<0.0001 and a mean bias -53.5 cell/μL, LOA -149.4-42.3 cell/μL). This<br />
study demonstrates that the CB method is suitable and more affordable for CD4 quantitation. This method is inexpensive and<br />
interchangeable with the latex bead-based methods for generating absolute counts in resource-limited areas.<br />
No.842<br />
Author (s) : Nantakomol, D., Nuchnoi, P.<br />
Title : Response from the authors<br />
Source : Laboratory Medicine, Volume 41, Issue 9, September <strong>2010</strong>, Page 567<br />
Document Type : Letter<br />
310
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : calibration, CD4 lymphocyte count, cell assay, human, Human immunodeficiency virus infected patient, letter, quantitative<br />
assay<br />
<strong>Abstract</strong> : –<br />
No.843<br />
Author (s) : Nantakomol, D., Nuchnoi, P., Noulsri, E., Lerdwana, S., Krisin, S., Chanprasert, S., Pattanapanyasat, K.<br />
Title : Enumeration <strong>of</strong> the absolute CD4 T-lymphocyte count by cell-bead assay<br />
Source : Cytometry Part B - Clinical Cytometry, Volume 78, Issue 4, July <strong>2010</strong>, Pages 260-266<br />
Document Type : Article<br />
Keywords : CD4 T-lymphocyte; Cell bead; Flow cytometry; Flow rate-based calibration; Trucount<br />
<strong>Abstract</strong> : Background: We have previously developed an alternative approach for undertaking absolute cell counting based upon flowrate<br />
calibration using cell bead (FCB), in which cell bead (CB) can be used as a flow-rate calibration material for generating the<br />
absolute microparticle counts. Here, we extended our work <strong>of</strong> counting CD4+ T-lymphocytes in HIV-infected blood samples<br />
with the FCB method. Methods: CD4+ T-lymphocyte counts in EDTA blood samples from 30 healthy subjects and 80 HIV-1infected<br />
patients were determined using TriTEST reagent. The absolute CD4+ T-lymphocytes were measured by FCB, and the<br />
results were compared with the absolute counting by commercial latex bead (CLB) or with flow rate-based calibration<br />
method (FR). Statistical correlation and agreement were analyzed using linear regression and Bland-Altman analysis. Results:<br />
There was no significant difference in the absolute number <strong>of</strong> CD4+ T-lymphocyte counts enumerated by FCB when<br />
compared with those two reference methods (CLB and FR). The absolute CD4+ T-lymphocyte counts obtained from FCB<br />
method was highly correlated with those obtained from CLB [r2= 0.99, y = 1.04x - 12.37, P < 0.001, and mean bias 11.96<br />
cell/μl, limit <strong>of</strong> agreement (LOA) -57.82 - 81.74 cell/μl], FR method (r2 = 0.98; y = 0.97x - 3.13, P < 0.001, and mean bias -<br />
24.15 cell/μl, LOA -114.44 - 66.13 cell/μl). Conclusions: The use <strong>of</strong> FCB is comparable with the use <strong>of</strong> CLB and FR. This<br />
approach showed the effective in reducing cost for generating the absolute CD4+ T-lymphocyte counts. Such an approach<br />
should facilitate and ensure the success <strong>of</strong> the ongoing antiretroviral therapy program in resource-limited countries. © <strong>2010</strong><br />
Clinical Cytometry Society.<br />
No.844<br />
Author (s) : Nantasenamat, C., Isarankura-Na-Ayudhya, C., Prachayasittikul, V.<br />
Title : Advances in computational methods to predict the biological activity <strong>of</strong> compounds<br />
Source : Expert Opinion on Drug Discovery, Volume 5, Issue 7, July <strong>2010</strong>, Pages 633-654<br />
Document Type : Review<br />
Keywords : Cheminformatics; Computational methods; Data mining; Machine learning; QSAR; Structure activity relationship<br />
<strong>Abstract</strong> : Importance <strong>of</strong> the field: The past decade had witnessed remarkable advances in computer science which had given rise to<br />
many new possibilities including the ability to simulate and model life's phenomena. Among one <strong>of</strong> the greatest gifts<br />
computer science had contributed to drug discovery is the ability to predict the biological activity <strong>of</strong> compounds and in doing<br />
so drives new prospects and possibilities for the development <strong>of</strong> novel drugs with robust properties. Areas covered in this<br />
review: This review presents an overview <strong>of</strong> the advances in the computational methods utilized for predicting the biological<br />
activity <strong>of</strong> compounds. What the reader will gain: The reader will gain a conceptual view <strong>of</strong> the quantitative structureactivity<br />
relationship paradigm and the methodological overview <strong>of</strong> commonly used machine learning algorithms. Take home<br />
message: Great advancements in computational methods have now made it possible to model the biological activity <strong>of</strong><br />
compounds in an accurate manner. To obtain such a feat, it is <strong>of</strong>ten necessary to forgo several data pre-processing and postprocessing<br />
procedures. A wide range <strong>of</strong> tools are available to perform such tasks; however, the proper selection and piecing<br />
together <strong>of</strong> complementary components in the prediction workflow remains a challenging and highly subjective task that<br />
heavily relies on the experience and judgment <strong>of</strong> the practitioner. © <strong>2010</strong> Informa UK Ltd.<br />
No.845<br />
Author (s) : Nantawanit, N., Chanchaichaovivat, A., Panijpan, B., Ruenwongsa, P.<br />
Title : Induction <strong>of</strong> defense response against Colletotrichum capsici in chili fruit by the yeast Pichia guilliermondii strain R13<br />
Source : Biological Control, Volume 52, Issue 2, February <strong>2010</strong>, Pages 145-152<br />
Document Type : Article<br />
Keywords : Biocontrol; Chili fruits; Colletotrichum capsici; Defense response; Induced resistance; Pichia guilliermondii<br />
311
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : Pichia guilliermondii strain R13, a yeast isolated from Thai rambutan, has been shown to suppress the fungal pathogen<br />
Colletotrichum capsici in harvested chili. Its multiple modes <strong>of</strong> action include nutrient competition, tight attachment to the<br />
fungus, and hydrolytic enzyme secretion. This study investigated the ability <strong>of</strong> the P. guilliermondii strain R13 to induce<br />
resistance against C. capsici in chili fruit. The pretreatment <strong>of</strong> chili with the yeast antagonist, physically separated from the<br />
fungus by known distances, significantly reduced disease incidence and lesion diameter caused by C. capsici. Compared to<br />
the controls, the yeast treatment also significantly enhanced the activities <strong>of</strong> phenylalanine ammonia-lyase, chitinase, and β-<br />
1,3-glucanase, and the accumulation <strong>of</strong> capsidiol phytoalexin in chili tissue. Scanning electron micrographs showed that the<br />
morphology <strong>of</strong> C. capsici spores and hyphae were abnormal and that the pathogen had restricted growth on chili tissue<br />
adjacent to the yeast-inoculated sites. The results indicate that the induction <strong>of</strong> resistance may be another mechanism by<br />
which the yeast antagonist suppresses C. capsici. © 2009 Elsevier Inc. All rights reserved.<br />
No.846<br />
Author (s) : Napolitano, M., Giansily-Blaizot, M., Dolce, A., Knudsen, J.B., Schved, J.F., Auerswald, G., Ingerslev, J., Shamsi, T.S., Altisent, C.,<br />
Chararoenkwan, P., Michaels, L., Chuansumrit, A., di Minno, G., Caliskan, U., Mariani, G.<br />
Title : Prophylaxis In Congenital Factor VII Deficiency, Indications, Efficacy and Safety: Results <strong>of</strong> the STER<br />
Source : BLOOD, Volume: 116, Issue: 21, Pages: 290-291, Published: NOV 19 <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.847<br />
Author (s) : Narjaikaew, P., Emarat, N., Arayathanitkul, K., Cowie, B.<br />
Title : MAGNETISM TEACHING SEQUENCES BASED ON AN INDUCTIVE APPROACH FOR FIRST-YEAR THAI UNIVERSITY SCIENCE STUDENTS<br />
Source : <strong>International</strong> Journal <strong>of</strong> Science and Mathematics Education, Volume 8, Issue 5, <strong>2010</strong>, Pages 891-910<br />
Document Type : Article<br />
Keywords : inductive approach; large class; students' understanding; teaching sequences<br />
<strong>Abstract</strong> : The study investigated the impact on student motivation and understanding <strong>of</strong> magnetism <strong>of</strong> teaching sequences based on<br />
an inductive approach. The study was conducted in large lecture classes. A pre- and post-Conceptual Survey <strong>of</strong> Electricity<br />
and Magnetism was conducted with just fewer than 700 Thai undergraduate science students, before and after being taught<br />
the concepts, in three academic years. For 2005 and 2006, overall, the students had a better understanding <strong>of</strong> concepts<br />
associated with electricity, but a majority <strong>of</strong> the students seemed not to understand magnetism after the teaching. Drawing<br />
on these findings, the teaching sequences <strong>of</strong> the magnetism topic were developed, and then implemented in the academic<br />
year 2007. The teaching sequences included demonstrations and visuals to help students infer rules and theories for<br />
themselves (inductive method). In addition, interactive notes, information on historical science discoveries about magnetic<br />
phenomenon, questions, student discussions, and magnetism problems were used to support student learning during<br />
lectures. Students in the academic year 2007 performed significantly better after the teaching. In addition, the students had a<br />
positive perception towards the teaching sequences, which allowed them to be involved more actively during lectures. ©<br />
<strong>2010</strong> National Science Council, Taiwan.<br />
No.848<br />
Author (s) : Nartthanarung, A., Thanapprapasr, D.<br />
Title : Comparison <strong>of</strong> outcomes for patients with cervical cancer who developed bone metastasis after the primary treatment with<br />
concurrent chemoradiation versus radiation therapy alone.<br />
Source : <strong>International</strong> journal <strong>of</strong> gynecological cancer : <strong>of</strong>ficial journal <strong>of</strong> the <strong>International</strong> Gynecological Cancer Society, Volume 20,<br />
Issue 8, November <strong>2010</strong>, Pages 1386-1390<br />
Document Type : Article<br />
Keywords : antineoplastic agent; adenocarcinoma, adjuvant therapy, adult, aged, article, bone tumor, comparative<br />
study, evaluation, female, human, metastasis, middle aged, multimodality cancer therapy, pathology, recurrent<br />
disease, retrospective study, squamous cell carcinoma, treatment outcome, uterine cervix tumor;<br />
Adenocarcinoma, Adult, Aged, Antineoplastic Combined Chemotherapy Protocols, Bone Neoplasms, Carcinoma, Squamous<br />
Cell, Combined Modality Therapy, Female, Humans, Middle Aged, Neoadjuvant Therapy, Radiotherapy,<br />
Adjuvant, Recurrence, Retrospective Studies, Treatment Outcome, Uterine Cervical Neoplasms<br />
312
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : The aims <strong>of</strong> this study were to retrospectively compare outcomes for patients with cervical cancer who developed bone<br />
metastasis later after the primary treatment at the time <strong>of</strong> diagnosis <strong>of</strong> cervical cancer with concurrent chemoradiation (CCRT)<br />
to radiation therapy alone (RT). We retrospectively analyzed the patients with cervical cancer during the period from January<br />
1998 to December 2007. Of these, 11 patients who received CCRT and 24 patients who received RT went on to develop bone<br />
metastasis. Among 4620 patients with cervical cancer, 51 patients had bone metastases. Sixteen patients were excluded<br />
including 10 patients with unavailable records and 6 patients who did not receive CCRT or RT at the time <strong>of</strong> diagnosis <strong>of</strong><br />
cervical cancer. Thirty-five patients who had bone metastasis received primary treatment with CCRT or RT. The 2 groups <strong>of</strong><br />
patients (CCRT vs RT) were similar in age, histologic cell type, and the <strong>International</strong> Federation <strong>of</strong> Gynecology and Obstetrics<br />
stages. The characteristics <strong>of</strong> bone metastasis in both groups were also not significantly different. The patients who received<br />
CCRT did not have a better overall survival than the patients who received RT (median, 19 vs 22 months; 95% confidence<br />
interval [CI], 14.68-23.32 vs 8.56-35.44). They were comparable in the interval from cervical cancer diagnoses to diagnoses <strong>of</strong><br />
bone metastasis (CCRT group: median, 14 months; 95% CI, 9.14-18.86; RT group: median; 15 months; 95% CI, 10.20-19.80) and<br />
the survival after diagnosis <strong>of</strong> bone metastasis between both groups (CCRT group: median, 4 months; 95% CI, 0.76-7.24; RT<br />
group: median, 7 months; 95% CI, 4.70-9.30). Our retrospective analysis showed that there were no differences in survival<br />
benefits observed between the patients with cervical cancer who developed bone metastases later after the primary<br />
treatment with CCRT and RT.<br />
No.849<br />
Author (s) : Nasveld, P.E., Ebringer, A., Elmes, N., Bennett, S., Yoksan, S., Aaskov, J., McCarthy, K., Kanesa-thasan, N., Meric, C., Reid, M.<br />
Title : Long term immunity to live attenuated Japanese encephalitis chimeric virus vaccine: Randomized, double-blind, 5-year phase<br />
II study in healthy adults<br />
Source : Human Vaccines, Volume 6, Issue 12, December <strong>2010</strong>, Pages 1038-1046<br />
Document Type : Article<br />
Keywords : Antibody persistence; Chimeric virus vaccine; Clinical study; Immunogenicity; Japanese encephalitis vaccine; Safety<br />
<strong>Abstract</strong> : In a randomized, double-blind study, 202 healthy adults were randomized to receive a live, attenuated Japanese encephalitis<br />
chimeric virus vaccine (JE-CV) and placebo 28 days apart in a cross-over design. A subgroup <strong>of</strong> 98 volunteers received a JE-CV<br />
booster at month 6. Safety, immunogenicity and persistence <strong>of</strong> antibodies to month 60 were evaluated. There were no<br />
unexpected adverse events (AEs) and the incidence <strong>of</strong> AEs between JE-CV and placebo were similar. There were three serious<br />
adverse events (SAE ) and no deaths. A moderately severe case <strong>of</strong> acute viral illness commencing 39 days after placebo<br />
administration was the only SAE considered possibly related to immunization. 99% <strong>of</strong> vaccine recipients achieved a<br />
seroprotective antibody titer ≥10 to JE-CV 28 days following the single dose <strong>of</strong> JE-CV and 97% were seroprotected at month<br />
6. Kaplan Meier analysis showed that after a single dose <strong>of</strong> JE-CV, 87% <strong>of</strong> the participants who were seroprotected at month<br />
6 were still protected at month 60. This rate was 96% among those who received a booster immunization at month 6. 95%<br />
<strong>of</strong> subjects developed a neutralizing titer ≥10 against at least three <strong>of</strong> the four strains <strong>of</strong> a panel <strong>of</strong> wild-type Japanese<br />
encephalitis virus (JEV) strains on day 28 after immunization. At month 60, that proportion was 65% for participants who<br />
received a single dose <strong>of</strong> JE-CV and 75% for the booster group. These results suggest that JE-CV is safe, well tolerated and<br />
that a single dose provides long-lasting immunity to wild-type strains. © <strong>2010</strong> Landes Bioscience.<br />
No.850<br />
Author (s) : Nateewattana, J., Trichaiyaporn, S., Saouy, M., Nateewattana, J., Thavornyutikarn, P., Pengchai, P., Choonluchanon, S.<br />
Title : Monitoring <strong>of</strong> arsenic in aquatic plants, water, and sediment <strong>of</strong> wastewater treatment ponds at the Mae Moh Lignite power<br />
plant, Thailand<br />
Source : Environmental Monitoring and Assessment, Volume 165, Issue 1-4, June <strong>2010</strong>, Pages 585-594<br />
Document Type : Article<br />
Keywords : Arsenic; Canna glauca (L.); Colocasia esculenta (L.) Schott; Eichhornia crassipes (Mart.) Solms.; Ipomoea aquatica<br />
Forsk.; Mae Moh; Phytoremediation; Typha angustifolia (L.); Wetland pond<br />
<strong>Abstract</strong> : Mae Moh is a risky area for arsenic contamination caused by the effluent from biowetland ponds in Mae Moh lignite-fuelled<br />
power plant. The objective <strong>of</strong> this study was to investigate the arsenic concentrations <strong>of</strong> Mae Moh biowetland ponds and<br />
determine the main factors which are important for arsenic phytoremediation in the treatment system. The result revealed<br />
that arsenic concentrations in the supernant were in the range <strong>of</strong> less than 1.0 μg As L-1 to 2.0 μg As L-1 while those in the<br />
sediment were in the range <strong>of</strong> 25-200 μg As kg soil-1. Both values were below the Thailand national standard <strong>of</strong> 0.25 mg As<br />
L-1 for water and 27 mg As kg soil-1 for the soil. Arsenic accumulation in the biomass <strong>of</strong> 5 aquatic plants at the biowetland<br />
313
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
ponds ranged from 123.83 to 280.53 mg As kgPlant-1. Regarding the result <strong>of</strong> regression analysis (R 2= 0.474 to 0.954), high<br />
concentrations <strong>of</strong> organic matter and other soluble ions as well as high pH value in the sediment could significantly enhance<br />
the removal <strong>of</strong> soluble arsenic in the wetland ponds. From the regression equation <strong>of</strong> accumulated arsenic concentration in<br />
each aquatic plant, Eichhornia crassipes (Mart.) Solms. (R 2= 0.954), Ipomoea aquatica Forsk. (R 2= 0.850), and Typha<br />
angustifolia (L.) (R 2= 0.841) were found to be preferable arsenic removers for wastewater treatment pond in the condition <strong>of</strong><br />
low Eh value and high content <strong>of</strong> solid phase EC and phosphorus. On the other hand, Canna glauca (L.) (R 2= 0.749)<br />
appeared to be favorable arsenic accumulator for the treatment pond in the condition <strong>of</strong> high Eh value and high<br />
concentration <strong>of</strong> soluble EC. © 2009 Springer Science+Business Media B.V.<br />
No.851<br />
Author (s) : Nawamawat, K., Sakdapipanich, J.T., Ho, C.C.<br />
Title : Effect <strong>of</strong> deproteinized methods on the proteins and properties <strong>of</strong> natural rubber latex during storage<br />
Source : Macromolecular Symposia, Volume 288, Issue 1, February <strong>2010</strong>, Pages 95-103<br />
Document Type : Conference Paper<br />
Keywords : Deproteinization; Gel formation; Natural rubber latex particle; Proteins<br />
<strong>Abstract</strong> : Three different methods <strong>of</strong> deproteinization, i.e. saponification, surfactant washing and enzymatic treatment were employed<br />
to unravel the effect <strong>of</strong> deproteinized on the properties <strong>of</strong> natural rubber (NR) latex. The cleavage <strong>of</strong> proteins in NR latex was<br />
found to proceed with concomitant formation <strong>of</strong> low molecular weight polypeptides. This results in a lowering in gel<br />
formation <strong>of</strong> the enzyme-treated latex, indicating modification <strong>of</strong> the remaining proteins at the rubber chain-end. Washing NR<br />
latex with surfactant would efficiently reduce and remove proteins from NR latex particles through denaturation and<br />
transferring them to the serum phase. The relatively stable gel formed during storage <strong>of</strong> surfactant-washed NR latex is an<br />
indication <strong>of</strong> the absence <strong>of</strong> branch formation <strong>of</strong> proteins at the rubber molecule terminal. Saponification by strong alkali<br />
would hydrolyze the proteins and phospholipids adsorbed on the latex particle surface. The reason <strong>of</strong> the significantly higher<br />
gel formed in saponified NR latex is still not clear. The present study shows that deproteinization treatments result in<br />
modification <strong>of</strong> the proteins at the surface <strong>of</strong> NR latex particles and also those freely-suspended in the serum. The cleavage<br />
or the denaturation <strong>of</strong> the rubber proteins during purification by washing has a pr<strong>of</strong>ound effect on the properties <strong>of</strong> the<br />
deproteinized NR latex upon storage, in particular the thermal oxidative aging properties <strong>of</strong> the rubber obtained. Copyright ©<br />
<strong>2010</strong> WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.<br />
No.852<br />
Author (s) : Nerungsi, C., Wanitchang, P., Sahasithiwat, S., Sadorn, K., Kerdcharoen, T., Thongpanchang, T.<br />
Title : Organic electroluminescence devices based on anthracene sulfide derivatives<br />
Source : Tetrahedron Letters, Volume 51, Issue 49, 8 December <strong>2010</strong>, Pages 6392-6395<br />
Document Type : Article<br />
Keywords : 9,10 bis(1 dodecylthio)anthracene, anthracene derivative, chalcogen, disulfide, oxygen, sulfur, unclassified drug;<br />
article, electrochemical analysis, light emitting diode, luminescence, synthesis<br />
<strong>Abstract</strong> : A series <strong>of</strong> anthracene derivatives are synthesized and fabricated as light-emitting materials in OLED devices. The<br />
incorporation <strong>of</strong> the chalcogen atoms, either oxygen or sulfur, in between the anthracene moiety and the alkyl or aryl<br />
substituents affected drastically the photo- and electroluminescence properties <strong>of</strong> the materials, especially the HOMO-LUMO<br />
band gap and the emitting color <strong>of</strong> the devices. The new anthracene sulfide derivatives represent a new design for further<br />
modification <strong>of</strong> other light-emitting doped materials. © <strong>2010</strong> Elsevier Ltd. All rights reserved.<br />
No.853<br />
Author (s) : Netcharoen, N., Limroongreungrat, W., Chottidao, M.<br />
Title : Comparison <strong>of</strong> Anaerobic Power between Weight Sled Resistance and Interval Trainings in Thai Athletes<br />
Source : MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, Volume: 42, Issue: 5, Supplement: 1, Pages: 291-292, Meeting <strong>Abstract</strong>:<br />
1487, Published: MAY <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
314
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.854<br />
Author (s) : Netikul, T., Leelaporn, A., Leelarasmee, A., Kiratisin, P.<br />
Title : In vitro activities <strong>of</strong> fosfomycin and carbapenem combinations against carbapenem non-susceptible Escherichia coli and<br />
Klebsiella pneumonia<br />
Source : <strong>International</strong> Journal <strong>of</strong> Antimicrobial Agents, Volume 35, Issue 6, June <strong>2010</strong>, Pages 609-610<br />
Document Type : Letter<br />
Keywords : carbapenem, doripenem, ertapenem, fosfomycin, imipenem, meropenem; antibiotic sensitivity, antimicrobial activity, bacterial<br />
strain, bacterium isolate, controlled study, disk diffusion, Escherichia coli, in vitro study, Klebsiella<br />
pneumoniae, letter, minimum inhibitory concentration, nonhuman, priority journal; Anti-Bacterial Agents, Carbapenems, Drug<br />
Resistance, Bacterial, Drug Synergism, Escherichia coli, Escherichia coli Infections, Fosfomycin, Hospitals, Humans, Klebsiella<br />
Infections, Klebsiella pneumoniae, Microbial Sensitivity Tests<br />
<strong>Abstract</strong> : –<br />
No.855<br />
Author (s) : Netsawang, J., Noisakran, S., Puttikhunt, C., Kasinrerk, W., Wongwiwat, W., Malasit, P., Yenchitsomanus, P.-t., Limjindaporn, T.<br />
Title : Nuclear localization <strong>of</strong> dengue virus capsid protein is required for DAXX interaction and apoptosis<br />
Source : Virus Research, Volume 147, Issue 2, February <strong>2010</strong>, Pages 275-283<br />
Document Type : Article<br />
Keywords : Apoptosis; Daxx; Dengue virus capsid protein; Nuclear localization<br />
<strong>Abstract</strong> : Dengue virus capsid protein (DENVC) localizes to both the cytoplasm and nucleus <strong>of</strong> dengue virus-infected cells. DENV C<br />
contains three nuclear localization signals (NLS), 6KKAR9, 73KKSK76, and the bipartite signal 85RKeigrmlnilnRRRR100. Stable<br />
HepG2 cells constitutively expressing DENV C, DENV C (∆85-100) and DENV C (∆73-100) were constructed to clarify whether<br />
nuclear translocation <strong>of</strong> DENV C affected apoptosis in liver cell line. While the wild-type DENV C could translocate into the<br />
nuclei <strong>of</strong> HepG2 cells, the mutant DENV Cs were restricted to the cytoplasm. The loss <strong>of</strong> nuclear localization <strong>of</strong> both mutant<br />
DENV Cs resulted in the disruption <strong>of</strong> their interactions with the apoptotic protein Daxx. Interestingly, upon treatment with<br />
anti-Fas antibody, the HepG2 cells expressing the wild-type DENV C showed significantly more apoptosis compared with the<br />
HepG2 cells expressing either mutant DENV C. To identify the amino acids required for DAXX interaction and apoptosis,<br />
substitution mutations either (K73A/K74A) or (R85A/K86A) were introduced into the C-terminal region <strong>of</strong> DENV C, and tested<br />
whether these mutations affected its interaction with Daxx and apoptosis. The results demonstrate that 73KK and 85RK <strong>of</strong><br />
DENV C are important for its nuclear localization, interaction with DAXX and induction <strong>of</strong> apoptosis. This work is the first to<br />
demonstrate that nuclear localization <strong>of</strong> DENV C is required for DAXX interaction and apoptosis. © 2009 Elsevier B.V. All rights<br />
reserved.<br />
No.856<br />
Author (s) : Nettuwakul, C., Sawasdee, N., Yenchitsomanus, P.<br />
Title : Rapid detection <strong>of</strong> solute carrier family 4, member 1 (SLC4A1) mutations and polymorphisms by high-resolution melting<br />
analysis<br />
Source : CLINICAL BIOCHEMISTRY, Volume: 43, Issue: 4-5, Pages: 497-504, DOI: 10.1016/j.clinbiochem.2009.12.010, Published: MAR <strong>2010</strong><br />
Document Type : Article<br />
Keywords : Solute carrier family 4 member A; SLC4A1; Anion exchanger 1; AE1; Band 3; Spherocytosis; Ovalocytosis; Distal renal tubular<br />
acidosis; dRTA; Real-time PCR; Single nuclectide polymorphism; SNP; Mutation; High-resolution melting; HRM analysis<br />
<strong>Abstract</strong> : Objective: The objective <strong>of</strong> this study is to develop and evaluate a high-resolution melting (HRM) method for detection <strong>of</strong><br />
SLC4A1 Mutations and polymorphisms. Design and methods: The HRM method was optimized for detection <strong>of</strong> 18 known<br />
SLC4A1 variants. It was then used for analysis <strong>of</strong> 16 blind DNA samples highly enriched with two common mutations,<br />
Southeast Asian ovalocytosis (SAO) and band 3 Bangkok 1 (G701D), to compare the results with that <strong>of</strong> the conventional<br />
procedures. Results: The HRM method was able to detect all IS SLC4A1 variants. In the samples in which homozygous wildtype<br />
and homozygous variant could not be distinguished by difference plots, they were spiked with a sample carrying known<br />
homozygous genotype, resulting in their clear differentiation. The HRM method had 100% efficiency for detection <strong>of</strong><br />
mutations in the blind DNA samples, when compared with that <strong>of</strong> the conventional techniques. Conclusions: The developed<br />
HRM method is efficient and reproducible for detection <strong>of</strong> SLC4A1 mutations and polymorphisms. (C) 2009 The Canadian<br />
Society <strong>of</strong> Clinical Chemists. Published by Elsevier Inc. All rights reserved.<br />
315
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.857<br />
Author (s) : Ngamjanyaporn P., Thakkinstian A., Verasertniyom O., Chatchaipun P., Vanichapuntu M., Nantiruj K., Totemchokchyakarn K.,<br />
Attia J., Janwityanujit S.<br />
Title : Pharmacogenetics <strong>of</strong> cyclophosphamide and CYP2C19 polymorphism in Thai systemic lupus erythematosus<br />
Source : Rheumatology <strong>International</strong>, Volume 31, Issue 9, September 2011, Pages 1215-1218<br />
Document Type : Article<br />
Keywords : Cyclophosphamide; CYP2C19; Ovarian toxicity; Pharmacogenetics; SLE (systemic lupus erythematosus)<br />
<strong>Abstract</strong> : To assess whether the CYP2C19 polymorphism modified the effect <strong>of</strong> cyclophosphamide on ovarian toxicity in Thai patients<br />
with SLE. We performed a case-control study <strong>of</strong> female patients with SLE who were treated with cyclophosphamide at<br />
Ramathibodi Hospital, Bangkok, Thailand. Cases were patient who had ovarian toxicity (sustained amenorrhoea >12 months or<br />
lack <strong>of</strong> menstruation for >4 months). CYP2C19 polymorphism was genotyped using PCR-RFLP method. Logistic regression was<br />
applied to assess CYP2C19 polymorphism as an effect modifier <strong>of</strong> cyclophosphamide. Seventy-one patients with SLE were<br />
enrolled, <strong>of</strong> which 36 (59.7%) had ovarian toxicity. CYP2C19*2 allele frequencies were 27.8 and 21.4% in the ovarian and nonovarian<br />
toxicity groups. Patients with CYP2C19*1/*1 genotype and higher cumulative dose <strong>of</strong> cyclophosphamide (>23.75 g)<br />
had the highest odds <strong>of</strong> ovarian toxicity, i.e. 11.0 (95% CI: 1.2-99.1) times higher than patients with the CYP2C19*1/ *2 or*2/*2<br />
genotypes who received less cyclophosphamide (
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
investigated. It was found that the mirror-image BC is a suitable boundary treatment for this Min-protein model. The physical<br />
significance <strong>of</strong> the results is extensively discussed. © <strong>2010</strong> Elsevier Inc. All rights reserved.<br />
No.860<br />
Author (s) : Ngamsaad, W., Yojina, J., Triampo, W.<br />
Title : Theoretical studies <strong>of</strong> phase-separation kinetics in a Brinkman porous medium<br />
Source : Journal <strong>of</strong> Physics A: Mathematical and Theoretical, Volume 43, Issue 20, <strong>2010</strong>, Article number 202001<br />
Document Type : Article<br />
Keywords : –<br />
<strong>Abstract</strong> : Although the coarsening <strong>of</strong> binary fluid mixtures in porous media has been <strong>of</strong> great interest for some time, there are still no<br />
complete theories for describing the relevant mechanisms, and more theoretical work needs to be carried out. In this work,<br />
we have proposed a simple model for phase separation <strong>of</strong> binary fluids in a porous medium, where the Brinkman-extended-<br />
Darcy equation and Cahn-Hilliard equation are the dynamical constitutions. Using the dimensional analysis approach, our<br />
findings lead to the prediction <strong>of</strong> domain coarsening in a porous medium for several regimes, including the conventional<br />
power laws. In addition, we have found that slowed-down coarsening dynamics are caused by the hydrodynamic screening<br />
effect, which is governed by the logarithmic law for this regime. Our theoretical results are at least qualitatively consistent<br />
with previous reports using simulations or experiments. © <strong>2010</strong> IOP Publishing Ltd.<br />
No.861<br />
Author (s) : Ngamsuriyaroj, S., Pornpattana, R.<br />
Title : Performance evaluation <strong>of</strong> TPC-H queries on MySQL cluster<br />
Source : 24th IEEE <strong>International</strong> Conference on Advanced Information Networking and Applications Workshops, WAINA <strong>2010</strong>, <strong>2010</strong>,<br />
Article number 5480961, Pages 1035-1040<br />
Document Type : Conference Paper<br />
Keywords : MySQL cluster; Query transformation; TPC-H benchmark<br />
<strong>Abstract</strong> : This paper evaluates the performance <strong>of</strong> TPC-H queries on MySQL cluster via our designed vParNDB middleware based on the<br />
storage engine called NDB. The middleware combines both inter-query and intra-query parallelism to provide better query<br />
performance over the original MySQL cluster. Many strategies such as query rewriting and SQL hints are applied to optimize<br />
query execution plans <strong>of</strong> all queries as they can be simultaneously executed as subqueries on each MySQL node before the<br />
final results are aggregated at the front end node. The performance <strong>of</strong> all 22 TPC-H queries is evaluated, and the results<br />
illustrate significant speedup as well as better throughput. © <strong>2010</strong> IEEE.<br />
No.862<br />
Author (s) : Ngaosuwankul, N., Noisumdaeng, P., Komolsiri, P., Pooruk, P., Chokephaibulkit, K., Chotpitayasunondh, T., Sangsajja, C.,<br />
Chuchottaworn, C., Farrar, J., Puthavathana, P.<br />
Title : Influenza A viral loads in respiratory samples collected from patients infected with pandemic H1N1, seasonal H1N1 and H3N2<br />
viruses<br />
Source : VIROLOGY JOURNAL, Volume: 7, Article Number: 75, DOI: 10.1186/1743-422X-7-75, Published: APR 20 <strong>2010</strong><br />
Document Type : Article<br />
Keywords : –<br />
<strong>Abstract</strong> : Background: Nasopharyngeal aspirate (NPA), nasal swab (NS), and throat swab (TS) are common specimens used for diagnosis<br />
<strong>of</strong> respiratory virus infections based on the detection <strong>of</strong> viral genomes, viral antigens and viral isolation. However, there is no<br />
documented data regarding the type <strong>of</strong> specimen that yields the best result <strong>of</strong> viral detection. In this study, quantitative real<br />
time RT-PCR specific for M gene was used to determine influenza A viral loads present in NS, NPA and TS samples collected<br />
from patients infected with the 2009 pandemic H1N1, seasonal H1N1 and H3N2 viruses. Various copy numbers <strong>of</strong> RNA<br />
transcripts derived from recombinant plasmids containing complete M gene insert <strong>of</strong> each virus strain were assayed by RT-<br />
PCR. A standard curve for viral RNA quantification was constructed by plotting each Ct value against the log quantity <strong>of</strong> each<br />
standard RNA copy number. Results: Copy numbers <strong>of</strong> M gene were obtained through the extrapolation <strong>of</strong> Ct values <strong>of</strong> the<br />
test samples against the corresponding standard curve. Among a total <strong>of</strong> 29 patients with severe influenza enrolled in this<br />
study ( 12 cases <strong>of</strong> the 2009 pandemic influenza, 5 cases <strong>of</strong> seasonal H1N1 and 12 cases <strong>of</strong> seasonal H3N2 virus), NPA was<br />
found to contain significantly highest amount <strong>of</strong> viral loads and followed in order by NS and TS specimen. Viral loads among<br />
patients infected with those viruses were comparable regarding type <strong>of</strong> specimen analyzed. Conclusion: Based on M gene<br />
317
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
copy numbers, we conclude that NPA is the best specimen for detection <strong>of</strong> influenza A viruses, and followed in order by NS<br />
and TS.<br />
No.863<br />
Author (s) : Ngarmukos, C., Chailurkit, L., Chanprasertyothin, S., Hengprasith, B., Sritara, P., Ongphiphadhanakul, B.<br />
Title : Baseline osteocalcin inversely predicts development <strong>of</strong> diabetes in a long-term follow-up cohort<br />
Source : ENDOCRINE JOURNAL, Volume: 57, Supplement: 2, Pages: S326-S326, Published: MAR <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.864<br />
Author (s) : Nguyen, T.H., Petchsuk, A., Tangboriboonrat, P., Opaprakasit, M., Sharp, A., Opaprakasit, P.<br />
Title : Synthesis and characterizations <strong>of</strong> PLLA/PEG block copolymers<br />
Source : Advanced Materials Research, Volume 93-94, <strong>2010</strong>, Pages 198-201<br />
Document Type : Conference Paper<br />
Keywords : Chain extending; Copolymer; Degradable polymer; PEG; PLLA<br />
<strong>Abstract</strong> : Poly(lactic acid-co-ethylene glycol) (PLLA/PEG) copolymers were synthesized and their properties were characterized. The<br />
PLLA/PEG/PLLA triblock copolymers were synthesized by ring-opening polymerization from l-lactide (LLA) and PEG<br />
macroinitiator. Stannous octoate, Sn(Oct)2 was used as a catalyst. Effects <strong>of</strong> molecular weight <strong>of</strong> PEG (600, 2000 and 4000),<br />
LLA/OH molar ratios (95:5, 98:2) and a sequence <strong>of</strong> addition <strong>of</strong> the reactants on properties <strong>of</strong> the copolymers were<br />
investigated. The triblock copolymers were subsequently used in a production <strong>of</strong> multiblock copolymers by reacting with a<br />
chain-extending agent, hexamethylene diisocyanate (HMDI). Chemical structure and molecular weight <strong>of</strong> the copolymers were<br />
characterized by 1HNMR, FTIR and GPC. The results showed that molecular weight <strong>of</strong> triblock copolymers varied from 4,500<br />
to 10,200. After chain extension, multiblock copolymer with molecular weight <strong>of</strong> 16,490 was produced. Thermal properties <strong>of</strong><br />
the copolymers were also examined by DSC. © (<strong>2010</strong>) Trans Tech <strong>Publications</strong>.<br />
No.865<br />
Author (s) : Nicolini, F.; Kim, D. W.; Turkina, A.; Alimena, G.; Al-Ali, H. K.; Shen, Z.; Jootar, S.; Smith, G.; De Souza, C.; Dorlhiac-Llacer, P.;<br />
Rizzieri, D.; Lyon-Caen, S.; Berton, M.; Szczudlo, T.; le Coutre, P.<br />
Title : ACTIVE DOSE RE-ESCALATION OF NILOTINIB FOLLOWING DOSE REDUCTION IS ASSOCIATED WITH IMPROVED EFFICACY IN<br />
PATIENTS WITH CHRONIC PHASE CHRONIC MYELOID LEUKEMIA (CML-CP) WHO FAIL IMATINIB<br />
Source : HAEMATOLOGICA-THE HEMATOLOGY JOURNAL, Volume: 95, Supplement: 2, Pages: 55-55, Meeting <strong>Abstract</strong>: 0137, Published:<br />
JUN <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.866<br />
Author (s) : Nijvipakul, S., Ballou, D.P., Chaiyen, P.<br />
Title : Reduction kinetics <strong>of</strong> a flavin oxidoreductase LuxG from photobacterium leiognathi (TH1): Half-sites reactivity<br />
Source : Biochemistry, Volume 49, Issue 43, 2 November <strong>2010</strong>, Pages 9241-9248<br />
Document Type : Article<br />
Keywords : Absorbances, Bi-Phasic, Burst kinetics, Flavin reductase, Functional forms, Kinetic data, Kinetic isotope effects, Labeled<br />
substrates, Monooxygenases, Overall reactions, Oxidoreductases, Photobacterium leiognathi, Pyridine nucleotides, Reactivity<br />
models, Reduction kinetics, Reduction phase, Second phase, Steady-state kinetics, Stopped-flow<br />
spectrophotometry, Transient kinetics, Turnover number, Two-component; Aldehydes, Bacteriology, Charge<br />
transfer, Coenzymes, Isotopes, Nucleotides, Plants (botany), Rate constants, Reaction intermediates, Spectrophotometry;<br />
Reaction kinetics; alkanal monooxygenase (FMN linked), flavine mononucleotide, isotope, oxidoreductase, pyridine<br />
nucleotide, quercetin, reduced nicotinamide adenine dinucleotide; article, bioluminescence, enzyme binding, enzyme<br />
kinetics, kinetics, nonhuman, operon, oxidation, Photobacterium leiognathi, priority journal, reduction<br />
kinetics, spectrophotometry, steady state; Bacterial Proteins, Catalysis, Flavin<br />
318
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Mononucleotide, Flavins, Kinetics, Luciferases, Luminescent Proteins, NAD, Oxidoreductases, Photobacterium, Spectrum<br />
Analysis; Bacteria (microorganisms), Photobacterium leiognathi<br />
<strong>Abstract</strong> : Bacterial bioluminescence is a phenomenon resulting from the reaction <strong>of</strong> a two-component FMN-dependent aldehyde<br />
monooxygenase system, which comprises a bacterial luciferase and a flavin reductase. Bacterial luciferase (LuxAB) is one <strong>of</strong><br />
the most extensively investigated two-component monooxygenases, while its reductase partner, the flavin reductase (LuxG)<br />
from the same operon, has only been recently expressed in a functional form. This work reports transient kinetics<br />
identification <strong>of</strong> intermediates in the LuxG reaction using stopped-flow spectrophotometry. The results indicate that the<br />
overall reaction follows a sequential-ordered mechanism in which NADH binds first to the enzyme, followed by FMN,<br />
resulting in the formation <strong>of</strong> charge-transfer intermediate 1 (CT-1) typical <strong>of</strong> those between reduced pyridine nucleotides and<br />
oxidized flavins. The next step is the reduction <strong>of</strong> FMN as indicated by a large decrease in absorbance at 450 nm. The<br />
reduction <strong>of</strong> FMN is biphasic. The first phase <strong>of</strong> FMN reduction occurs concurrently with formation <strong>of</strong> charge-transfer<br />
intermediate 2 (CT-2), while the second phase is synchronous with the decay <strong>of</strong> CT-2. When the isotope-labeled substrate,<br />
4(R)-[2H]NADH, was used, the first reduction phase showed a primary kinetic isotope effect (Dk red) <strong>of</strong> ≥3.9 and resulted in<br />
greater accumulation <strong>of</strong> CT-1. These results are consistent with CT-1 being the FMNox:NADH complex, while CT-2 is the<br />
FMNred:NAD+ complex. Because CT-2 decays with a rate constant <strong>of</strong> 2.8 ± 0.2 s-1, while the turnover number obtained from<br />
the steady-steady-state kinetics is 1.7 s-1, it is likely that the CT-2 decay step largely controls the overall reaction rate. All<br />
kinetic data are consistent with a half-sites reactivity model in which flavin reduction occurs at only one subunit at a time.<br />
The first reduction phase is due to the reduction <strong>of</strong> FMN in the first subunit, while the second phase is due to the reduction<br />
<strong>of</strong> FMN in the second subunit. The latter phase is limited by the rate <strong>of</strong> decay <strong>of</strong> CT-2 in the first subunit. The half-sites<br />
reactivity model is also supported by detection <strong>of</strong> burst kinetics during the pre-steady-state period that is correlated with 0.5<br />
mol <strong>of</strong> the FMN being reduced/mol <strong>of</strong> the LuxG:NADH complex. The functional importance <strong>of</strong> this half-site reactivity<br />
phenomenon is still unclear. © <strong>2010</strong> American Chemical Society.<br />
No.867<br />
Author (s) : Nilanont, Y., Komoltri, C., Saposnik, G., Côté, R., Di Legge, S., Jin, Y., Prayoonwiwat, N., Poungvarin, N., Hachinski, V.<br />
Title : The canadian neurological scale and the NIHSS: Development and validation <strong>of</strong> a simple conversion model<br />
Source : Cerebrovascular Diseases, Volume 30, Issue 2, July <strong>2010</strong>, Pages 120-126<br />
Document Type : Article<br />
Keywords : Acute stroke; Canadian Neurological Scale; Conversion model; National Institutes <strong>of</strong> Health Stroke Scale; Neurological<br />
scales; Stroke severity assessment<br />
<strong>Abstract</strong> : Background: The Canadian Neurological Scale (CNS) and the National Institutes <strong>of</strong> Health Stroke Scale (NIHSS) are among the<br />
most reliable stroke severity assessment scales. The CNS requires less extensive neurological evaluation and is quicker and<br />
simpler to administer. Objective: Our aim was to develop and validate a simple conversion model from the CNS to the NIHSS.<br />
Methods: A conversion model was developed using data from a consecutive series <strong>of</strong> acute-stroke patients who were scored<br />
using both scales. The model was then validated in an external dataset in which all patients were prospectively assessed for<br />
stroke severity using both scales by different observers which consisted <strong>of</strong> neurology residents or stroke fellows. Results: In<br />
all, 168 patients were included in the model development, with a median age <strong>of</strong> 73 years (20-94). Men constituted 51.8%.<br />
The median NIHSS score was 6 (0-31). The median CNS score was 8.5 (1.5-11.5). The relationship between CNS and NIHSS<br />
could be expressed as the formula: NIHSS = 23 - 2 × CNS. A cohort <strong>of</strong> 350 acute-stroke patients with similar characteristics<br />
was used for model validation. There was a highly significant positive correlation between the observed and predicted NIHSS<br />
score (r = 0.87, p < 0.001). The predicted NIHSS score was on average 0.61 higher than the observed NIHSS score (95% CI =<br />
0.31-0.91). Conclusions: The CNS can be reliably converted to the NIHSS using a simple conversion formula: NIHSS = 23 - 2 ×<br />
CNS. This finding may have a practical impact by permitting reliable comparisons with NIHSS-based evaluations and<br />
simplifying the routine assessment <strong>of</strong> acute-stroke patients in more diverse settings. Copyright © <strong>2010</strong> S. Karger AG, Basel.<br />
No.868<br />
Author (s) : Nilanont, Y., Phattharayuttawat, S., Chiewit, P., Chotikanuchit, S., Limsriwilai, J., Chalernpong, L., Yamkaew, N., Lirathpong, N.,<br />
Anprasertporn, P., Komoltri, C., Prayoonwiwat, N., Poungvarin, N.<br />
Title : Establishment <strong>of</strong> the Thai version <strong>of</strong> National Institute <strong>of</strong> Health Stroke Scale (NIHSS) and a validation study.<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand = Chotmaihet thangphaet, Volume 93 Suppl 1, January <strong>2010</strong>, Pages S171-178<br />
Document Type : Article<br />
319
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : adult, aged, article, Asian, brain ischemia, classification, cultural factor, ethnology, female, hospitalization, human, language,<br />
male, middle aged, national health organization, nomenclature, nuclear magnetic resonance imaging, observer variation,<br />
pathophysiology, prospective study, reproducibility, stroke, Thailand, United States, validation study; Adult, Aged, Aged, 80<br />
and over, Asian Continental Ancestry Group, Brain Ischemia, Cross-Cultural Comparison, Female, Humans, Language, Magnetic<br />
Resonance Imaging, Male, Middle Aged, National Institutes <strong>of</strong> Health (U.S.), Observer Variation, Prospective<br />
Studies, Reproducibility <strong>of</strong> Results, Severity <strong>of</strong> Illness Index, Stroke, Terminology as Topic, Thailand, United States<br />
<strong>Abstract</strong> : BACKGROUND: The National Institute <strong>of</strong> Health Stroke Scale (NIHSS) is a well validated stroke severity assessment scale. The<br />
scale is used to assess neurological deficits, progression and degree <strong>of</strong> recovery in acute stroke settings. To date, no Thai<br />
version exists. OBJECTIVE: This study aimed to adapt and validate a Thai version <strong>of</strong> the NIHSS (NIHSS-T). MATERIAL AND<br />
METHOD: A cross-cultural adaptation <strong>of</strong> the NIHSS was developed according to the methods recommended by the<br />
<strong>International</strong> Quality <strong>of</strong> Life Assessment Project Group. Forward and backward translations were performed. A final version <strong>of</strong><br />
the NIHSS-T was validated against initial MRI infarction volume and modified Rankin Scale (mRS) at 3 months in a consecutive<br />
series <strong>of</strong> acute stroke patients. The patients were prospectively evaluated by 3 different types <strong>of</strong> health care providers: 2<br />
stroke fellows, 2 internists, and 2 stroke nurses. Mean NIHSS-T scores from all raters were used in the analysis. RESULTS: The<br />
study included 32 acute ischemic stroke patients with a mean age (+/- SD) <strong>of</strong> 64.53 +/- 14.97 years <strong>of</strong> age. Men comprised<br />
71.9%. Mean NIHSS-T score (+/- SD) was 7.49 +/- 7.02. Intra-observer reliability demonstrated a high agreement with an<br />
intraclass correlation (ICC) <strong>of</strong> 0.98, 0.98, 0.96, 0.98, 0.90 and 0.98 for 2 stroke fellows, 2 internists and 2 stroke nurses<br />
respectively. Inter-observer reliability between 6 raters was excellent, i.e.; ICC, 0.99 (0.98, 0.99). Spearman rank correlation<br />
coefficients between the initial NIHSS-T score versus initial MRI lesion volume and mRS at 3 months were 0.53 and 0.69 with a<br />
p-value <strong>of</strong> 0.002 and < 0.001 respectively. CONCLUSION: The Thai version <strong>of</strong> NIHSS is valid for assessing acute stroke severity.<br />
The scale is also reliable when administered in a Thai-speaking setting by trained healthcare pr<strong>of</strong>essionals.<br />
No.869<br />
Author (s) : Nillawong, M., Sombatsompop, N., Sirisinha, C.<br />
Title : Viscoelastic properties <strong>of</strong> nitrile rubber filled with lignite fly ash<br />
Source : Journal <strong>of</strong> Applied Polymer Science, Volume 116, Issue 6, 15 June <strong>2010</strong>, Pages 3497-3502<br />
Document Type : Article<br />
Keywords : Ash; Nitrile rubber; Reinforcement; Rheology; Viscoelastic behavior<br />
<strong>Abstract</strong> : Nitrile rubber (NBR) compounds containing various loadings <strong>of</strong> fly ash (FA) were prepared, and their viscoelastic properties and<br />
reinforcement mechanism were investigated and compared with those <strong>of</strong> natural rubber (NR) compounds reported<br />
previously. The results obtained exhibit an increase in storage modulus (G′) with increasing FA particularly at high FA loading.<br />
By contrast, the broadness <strong>of</strong> linear viscoelastic (LVE) region is found to decrease. With the use <strong>of</strong> Guth-Gold equation, the<br />
positive deviation <strong>of</strong> experimental values <strong>of</strong> relative modulus outward the theoretical values is observed at low strain <strong>of</strong><br />
deformation. However, at high deformation strain the negative deviation is found. The results imply that the presence <strong>of</strong><br />
pseudo-network (as formed via FA-FA and FA-NBR interactions) and the ball bearing effect provided by FA having spherical<br />
shape are responsible for the reinforcement in FA filled NBR compounds. © <strong>2010</strong> Wiley Periodicals, Inc.<br />
No.870<br />
Author (s) : Nimanong, S.; Siwaporn, C.; Sansak, I.; Tanwandee, T.; Charatcharoenwitthaya, P.; Chotiyaputta, W.; Boonyapisit, K.; Pausawasdi,<br />
N.; Prachayakul, W.; Pongprasobchai, S.; Leelakusolvong, S.; Manatsathit, S.; Kachintorn, U.<br />
Title : ORAL L-ORNITHINE-L-ASPARTATE (LOLA) FOR PATIENTS WITH OVERT HEPATIC ENCEPHALOPATHY TREATED WITH LACTULOSE; A<br />
RANDOMIZED, DOUBLE-BLINDED, PLACEBO-CONTROLLED TRIAL<br />
Source : JOURNAL OF HEPATOLOGY, Volume: 52, Supplement: 1, Pages: S78-S78, Published: <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.871<br />
Author (s) : Nimitphak, T., Meemetta, W., Arunrut, N., Senapin, S., Kiatpathomchai, W.<br />
Title : Rapid and sensitive detection <strong>of</strong> Penaeus monodon nucleopolyhedrovirus (PemoNPV) by loop-mediated isothermal<br />
amplification combined with a lateral-flow dipstick<br />
Source : Molecular and Cellular Probes, Volume 24, Issue 1, February <strong>2010</strong>, Pages 1-5<br />
320
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Document Type : Review<br />
Keywords : LAMP; Lateral-flow dipstick; LFD; Loop-mediated isothermal amplification; MBV; Monodon<br />
baculovirus; PCR; PemoNPV; Penaeus monodon; Penaeus monodon nucleopolyhedrovirus; Shrimp<br />
<strong>Abstract</strong> : Several methods such as traditional PCR or nested-PCR, immuno assay and histopathology have been developed for<br />
detection <strong>of</strong> Penaeus monodon nucleopolyhedrovirus (PemoNPV) formerly called monodon baculovirus (MBV). However,<br />
these methods have various disadvantages including low sensitivity, long assay time, use <strong>of</strong> toxic substances or unsuitability<br />
for field diagnosis. Loop-mediated isothermal amplification <strong>of</strong> target nucleotide sequences under isothermal conditions,<br />
combined with amplicon detection by chromatographic lateral-flow dipsticks allows for more efficient, field friendly detection<br />
within 75 min (not including DNA preparation time). In this study, the LAMP amplicon was biotinylated via an inner LAMP<br />
primer designed from a BamHI fragment B, a hypothetical protein gene <strong>of</strong> PemoNPV under isothermal condition at 63 °C for<br />
1 h. Next, the LAMP product was hybridized at 63 °C for 5 min with an optimal FITC-labeled probe that was designed<br />
specifically for the LAMP amplicons. The FITC-labeled biotinylated LAMP product picked up gold-labeled, anti-FITC near the<br />
LFD origin and the whole, triple-labeled complex was captured by an immobilized biotin-binding protein to yield a red nanogold<br />
stripe at the LFD test line. With a DNA template extracted from PemoNPV-infected shrimp, the LAMP-LFD detection limit<br />
was 0.1 pg, whereas one-step PCR and nested-PCR followed with gel electrophoresis was 1 pg. The LAMP-LFD method gave<br />
negative test results with buffer and DNA from shrimp infected with other common shrimp DNA viruses including, Penaeus<br />
monodon densovirus (PmDNV) formerly called hepatopancreatic parvovirus (HPV), white spot syndrome virus (WSSV) and<br />
Penaeus stylirostris densovirus (PstDNV) formerly called infectious hypodermal and hematopoietic necrosis virus (IHHNV). The<br />
test platform can be adapted easily for rapid detection <strong>of</strong> other shrimp viruses, since the LAMP-LFD combination system was<br />
a highly sensitive, specific, convenient, and does not require sophisticated instruments. © 2009 Elsevier Ltd. All rights<br />
reserved.<br />
No.872<br />
Author (s) : Nintasen, R.; Maneerat, Y.; Chartiburus, P.; Punsawad, C.; Khachansaksumeth, V.; Viriyavejakul, P.; Chaisri, U.<br />
Title : EFFECTS OF TUMOR NECROSIS FACTOR (TNF)-ALPHA AND 17-BETA ESTRADIOL (E(2)) ON PATHOLOGICAL CHANGES OF INTIMAL<br />
HYPERPLASIA<br />
Source : ATHEROSCLEROSIS SUPPLEMENTS, Volume: 11, Issue: 2, Pages: 144-144, Meeting <strong>Abstract</strong>: MS173, Published: JUN 10 <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.873<br />
Author (s) : Niparuck, P., Kanoksil, W., Chuncharunee, S., Boonsakan, P., Ungkanont, A., Angchaisuksiri, P., Karntisaviwat, K., Apilugsanachit,<br />
A., Rerkamnuatchoke, B., Jootar, S., Nitiyanant, P., Atichartakarn, V.<br />
Title : Therapy-related myelodysplastic syndrome/acute myeloid leukemia following fludarabine therapy for non-Hodgkin<br />
lymphoma and chronic lymphocytic leukemia in Thai patients<br />
Source : Leukemia and Lymphoma, Volume 51, Issue 11, November <strong>2010</strong>, Pages 2120-2125<br />
Document Type : Letter<br />
Keywords : 5 aza 2' deoxycytidine, carboplatin, cisplatin, cyclophosphamide, cytarabine, dexamethasone, doxorubicin, etoposide,<br />
fludarabine, ifosfamide, methotrexate, methylprednisolone, rituximab, vincristine; acute granulocytic leukemia, adult, anemia,<br />
cancer combination chemotherapy, cancer patient, cancer radiotherapy, cancer relapse, cancer staging, chronic lymphatic<br />
leukemia, clinical article, dyserythropoiesis, eosinophilia, female, follicular lymphoma, hairy cell leukemia, human, letter,<br />
lymphocytoma, male, mantle cell lymphoma, marginal zone lymphoma, mean corpuscular volume, myelodysplastic<br />
syndrome, neutropenia, nonhodgkin lymphoma, pancytopenia, priority journal, Thailand, thrombocytopenia, treatment<br />
duration; Adult, Aged, Antineoplastic Agents, Female, Humans, Leukemia, Lymphocytic, Chronic, B-Cell, Leukemia, Myeloid,<br />
Acute, Lymphoma, Non-Hodgkin, Male, Middle Aged, Myelodysplastic Syndromes, Neoplasms, Second Primary, Prognosis,<br />
Retrospective Studies, Thailand, Vidarabine, Young Adult<br />
<strong>Abstract</strong> : –<br />
No.874<br />
Author (s) : Niparuck, P., Sorakhunpipitkul, L., Atichartakarn, V., Chuncharunee, S., Ungkanont, A., Aungchaisuksiri, P., Puavilai, T., Jootar, S.<br />
321
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Treatment outcome <strong>of</strong> thalidomide based regimens in newly diagnosed and relapsed/refractory non-transplant multiple<br />
myeloma patients: A single center experience from Thailand<br />
Source : Journal <strong>of</strong> Hematology and Oncology, Volume 3, <strong>2010</strong>, Article number 1<br />
Document Type : Article<br />
Keywords : acetylsalicylic acid, dexamethasone, doxorubicin, melphalan, prednisolone, thalidomide, warfarin, antineoplastic agent;<br />
adult, article, clinical article, constipation, controlled study, deep vein thrombosis, disease course, drug<br />
mechanism, female, follow up, human, infection, male, multiple myeloma, muscle<br />
weakness, neuropathy, relapse, remission, Thailand, thrombosis, treatment duration, treatment outcome, adjuvant<br />
therapy, aged, drug effect, drug resistance, evaluation, middle aged, mortality, pathology, recurrent disease, retrospective<br />
study, salvage therapy, survival, transplantation; Adult, Aged, Antineoplastic Combined Chemotherapy Protocols, Drug<br />
Resistance, Neoplasm, Female, Humans, Male, Middle Aged, Multiple Myeloma, Neoadjuvant<br />
Therapy, Recurrence, Retrospective Studies, Salvage Therapy, Survival<br />
Analysis, Thailand, Thalidomide, Transplantation, Treatment Outcome<br />
<strong>Abstract</strong> : Background. Thalidomide based regimen is an effective and well tolerated therapy in multiple myeloma (MM) patients,<br />
however, there were a small number <strong>of</strong> studies written about the results <strong>of</strong> thalidomide therapy in non-transplant MM<br />
patients. We therefore conducted a retrospective study <strong>of</strong> 42 consecutive patients with newly diagnosed and<br />
relapsed/refractory MM treated with thalidomide- based induction regimens followed by thalidomide maintenance therapy.<br />
Results. Induction regimens with thalidomide and dexamethasone, and the oral combination <strong>of</strong> melphalan, prednisolone and<br />
thalidomide were administrated in 22 and 16 patients, respectively. The remaining 4 patients received other thalidomide-<br />
containing regimens. Twenty-nine patients received thalidomide as a salvage regimen. Twenty-three out <strong>of</strong> 26 patients<br />
achieving complete remission (CR) and very good partial remission (VGPR) received thalidomide maintenance. Of the 41<br />
evaluable patients, median time <strong>of</strong> treatment was 21 months (3- 45 months), ORR was 92.7% with a 63.4% CR/VGPR. With a<br />
median follow up <strong>of</strong> 23 months, 3-year- PFS and 3-year-OS were 58.6 and 72.6%, respectively. Median time to progression<br />
was 42 months. While 3-year-PFS and 3-year-OS in non-transplant patients receiving thalidomide maintenance therapy were<br />
67 and 80%, respectively. Conclusions. Prolonged thalidomide therapy enhanced survival rate and less frequently developed<br />
serious toxicity in non-transplant multiple myeloma patients. © <strong>2010</strong> Niparuck et al; licensee BioMed Central Ltd.<br />
No.875<br />
Author (s) : Nirachanon, A., Smith, D.R.<br />
Title : A case study <strong>of</strong> factors influencing employment satisfaction in a thai life sciences research institute<br />
Source : Scientific Research and Essays, Volume 5, Issue 13, 4 July <strong>2010</strong>, Pages 1697-1701<br />
Document Type : Article<br />
Keywords : Biotechnology; Questionnaire; Research; Support staff; Thailand<br />
<strong>Abstract</strong> : The objective <strong>of</strong> this study was to find the level <strong>of</strong> employment satisfaction in a life sciences research institute staffed by a<br />
mix <strong>of</strong> academic and non-academic staff. Both academic and non-academic staff members were included in the survey. The<br />
study population comprised 121 employees at the Institute <strong>of</strong> Science and Technology for Research and Development,<br />
<strong>Mahidol</strong> <strong>University</strong>. The instrument for gathering data was in the form <strong>of</strong> a questionnaire consisting <strong>of</strong> 27 questions as well as<br />
5 basic demographic questions determining sex, age, level <strong>of</strong> education, rate <strong>of</strong> salary and working duration. Employment<br />
satisfaction was measured on a five point scale in terms <strong>of</strong> career progression, employment stability, job characteristic,<br />
relationship with colleagues, relationship to immediate supervisor and the physical work environment. Data were analyzed in<br />
both univariate and multivariate analyses. From the 121 questionnaires given out, 105 completed questionnaires were<br />
returned (86.78%). The majorities <strong>of</strong> the study population were females, and aged between 31 - 40 years old. In terms <strong>of</strong><br />
education, the majority <strong>of</strong> respondents had a Bachelor's degree, earned between 10,001 - 20,000 Baht and had been<br />
employed for between 11 and 20 years. Overall, it was determined that the level <strong>of</strong> satisfaction was moderate, since the<br />
relationship with colleagues showed the highest level <strong>of</strong> satisfaction and career progression showed the lowest. In particular,<br />
longer serving staff members were more likely to feel that they were underserved in the provision <strong>of</strong> short, pr<strong>of</strong>essional<br />
training sources. This is the first analysis <strong>of</strong> staff from a research institute in a developing country. Although, relatively simple<br />
in format, it may have application in other developing and under-developed countries as a quick and simple screening tool to<br />
determine the well being <strong>of</strong> the employees in research institutes. © <strong>2010</strong> Academic Journals.<br />
No.876<br />
Author (s) : Nitiyanant, W.<br />
322
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Epidemiology <strong>of</strong> diabetes and metabolic syndrome in Thailand<br />
Source : ENDOCRINE JOURNAL, Volume: 57, Supplement: 2, Pages: S313-S313, Published: MAR <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.877<br />
Author (s) : Niwattayakul, K., Kaewtasi, S., Chueasuwanchai, S., Hoontrakul, S., Chareonwat, S., Suttinont, C., Phimda, K., Chierakul, W.,<br />
Silpasakorn, S., Suputtamongkol, Y.<br />
Title : An open randomized controlled trial <strong>of</strong> desmopressin and pulse dexamethasone as adjunct therapy in patients with<br />
pulmonary involvement associated with severe leptospirosis<br />
Source : Clinical Microbiology and Infection, Volume 16, Issue 8, August <strong>2010</strong>, Pages 1207-1212<br />
Document Type : Article<br />
Keywords : Desmopressin; Dexamethasone; Leptospirosis; Pulmonary involvement<br />
<strong>Abstract</strong> : Pulmonary involvement in leptospirosis is emerging as a common complication <strong>of</strong> severe leptospirosis. A prospective<br />
randomized controlled trial <strong>of</strong> desmopressin or high-dose (pulse) dexamethasone as adjunctive therapy in 68 patients with<br />
pulmonary involvement associated with severe leptospirosis was conducted between July 2003 and October 2006 at five<br />
hospitals in Thailand. There were 23 patients in the desmopressin group, 22 in the pulse dexamethasone group, and 23 in a<br />
control group who received standard critical care alone. The diagnosis <strong>of</strong> leptospirosis was confirmed in 52 patients (77%).<br />
There were 15 deaths (22%), <strong>of</strong> which eight patients received desmopressin, four patients received pulse dexamethasone,<br />
and three patients received critical care alone (p 0.19). Eight patients with confirmed leptospirosis died (five patients in the<br />
desmopressin group, one in the pulse dexamethasone group and two in the control group). The mortality was not<br />
significantly different in the desmopressin group or pulse dexamethasone group compared to the control group in both<br />
intention-to-treat patients, and in patients with confirmed leptospirosis. There were no serious events associated with<br />
desmopressin treatment, although pulse dexamethasone treatment was associated with a significant increase in nosocomial<br />
infection. The results <strong>of</strong> logistic regression analysis revealed that serum bilirubin level was the only significant risk factor<br />
associated with mortality (OR 0.759, 95% CI 0.598-0.965, p 0.024). The results obtained in the present study do not support<br />
the use <strong>of</strong> either pulse dexamethasone or desmopressin as adjunct therapy for pulmonary involvement associated with<br />
severe leptospirosis. © <strong>2010</strong> The Authors. Journal Compilation © <strong>2010</strong> European Society <strong>of</strong> Clinical Microbiology and<br />
Infectious Diseases.<br />
No.878<br />
Author (s) : Niyomphol, W., Ruenwongsa, P.<br />
Title : Application <strong>of</strong> text-based math braille translation s<strong>of</strong>tware in learning mathematics<br />
Source : <strong>International</strong> Journal <strong>of</strong> Learning, Volume 17, Issue 3, <strong>2010</strong>, Pages 597-608<br />
Document Type : Article<br />
Keywords : Braille; Mathematics; NEMETH; Translation; Visually impaired<br />
<strong>Abstract</strong> : This research project involves the development <strong>of</strong> a text-based math Braille translation s<strong>of</strong>tware to support learning in<br />
mathematics. The researcher collected 192 math symbols from Nemeth Braille code resources. Nemeth code was used as<br />
the basis <strong>of</strong> text-based math symbols design for regular print text entry in any text editor. The math translation s<strong>of</strong>tware was<br />
developed to convert these text-based math symbols to Braille. This s<strong>of</strong>tware can be interfaced to Braille s<strong>of</strong>tware for other<br />
languages. The s<strong>of</strong>tware was tested for its performance with 10 subjects who are math teachers and material production staff<br />
in Thailand. They were given 3 hours <strong>of</strong> training on the use <strong>of</strong> the s<strong>of</strong>tware and 2 hours to work on an exercise. Overall<br />
performance <strong>of</strong> the exercise was 95.32 percent accuracy. Based on the questionnaire after the exercise, overall satisfaction<br />
level <strong>of</strong> using this method was between the range <strong>of</strong> "acceptable and "good". A follow-up interview reflects the opinion that<br />
this method would be most appropriate for math teachers and material production staff who are not familiar with Braille.<br />
This s<strong>of</strong>tware would provide greater access toward math education <strong>of</strong> children with visual impairment. © Common Ground,<br />
Wiraman Niyomphol, Pintip Ruenwongsa.<br />
No.879<br />
Author (s) : Noisakran, S., Onlamoon, N., Pattanapanyasat, K., Hsiao, H.M., Chokephaibulkit, K., Perng, G.C.<br />
Title : THROMBOCYTOPENIA IN DENGUE PATIENTS: THE EFFECTS OF BODY TEMPERATURE AND CIRCULATING CD41(+)CD61(+) CELLS<br />
323
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Source : AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, Volume: 83, Issue: 5, Supplement: S, Pages: 153-154, Meeting<br />
<strong>Abstract</strong>: 511, Published: NOV <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.880<br />
Author (s) : Noisopa, C., Prapagdee, B., Navanugraha, C., Hutacharoen, R.<br />
Title : Effects <strong>of</strong> bio-extracts on the growth <strong>of</strong> Chinese kale<br />
Source : Kasetsart Journal - Natural Science, Volume 44, Issue 5, September <strong>2010</strong>, Pages 808-815<br />
Document Type : Article<br />
Keywords : Bio-extract; Chinese cabbage; Chinese kale; Growth promotion; Soybean<br />
<strong>Abstract</strong> : The effectiveness <strong>of</strong> three bio-extracts: Chinese kale, Chinese cabbage and soybean on Chinese kale growth was compared.<br />
The nutrient composition and chemical properties <strong>of</strong> the three bio-extracts were investigated. The various concentrations <strong>of</strong><br />
each bio-extract were applied daily to the supporting media, to enhance Chinese kale growth. The height, fresh weight and<br />
dry weight <strong>of</strong> the Chinese kale were measured for both stems and leaves. The results showed that the acidic condition <strong>of</strong> all<br />
bio-extracts had a high electrical conductivity (EC). However, the nutrient concentration in each bio-extract was noted to be<br />
less than that <strong>of</strong> the complete nutrient solution. Furthermore, soybean bio-extract performed the best in the most acidic<br />
conditions, had the highest EC values, and the greatest amount <strong>of</strong> total nutrients. Chinese kale and Chinese cabbage bioextracts<br />
performed similarly in terms <strong>of</strong> chemical characteristics. With regard to growth enhancement using these bio-extracts<br />
with determined concentrations, a complete nutrient solution gave the best height and both fresh and dry weights, and<br />
showed a significant statistical difference from the other two bio-extracts. Soybean bio-extract was the most effective bioextract<br />
to enhance growth.<br />
No.881<br />
Author (s) : Nopparat, C., Porter, J.E., Ebadi, M., Govitrapong, P.<br />
Title : The mechanism for the neuroprotective effect <strong>of</strong> melatonin against methamphetamine-induced autophagy<br />
Source : Journal <strong>of</strong> Pineal Research, Volume 49, Issue 4, November <strong>2010</strong>, Pages 382-389<br />
Document Type : Article<br />
Keywords : apoptosis; autophagy; Bcl-2; Beclin 1; melatonin; methamphetamine; reactive oxygen species<br />
<strong>Abstract</strong> : Methamphetamine (METH) is a common drug <strong>of</strong> abuse that induces toxicity in the central nervous system and is connected<br />
to neurological disorders such as Parkinsons disease. METH neurotoxicity is induced by reactive oxygen species (ROS)<br />
production and apoptosis. Moreover, autophagy is an alternative to cell death and a means for eliminating dysfunctional<br />
organelles. In other cases, autophagy can end up in cell death. Nonetheless, it is not clear whether autophagy is also<br />
correlated with apoptotic signaling in drug-induced neurotoxicity. Therefore, we hypothesized that METH-generated toxicity<br />
associated with initiating the apoptotic signaling cascade can also increase the autophagic phenotype in neuronal cells. Using<br />
the SK-N-SH dopaminergic cell line as our model system, we found that METH-induced autophagy by inhibiting dissociation <strong>of</strong><br />
Bcl-2/Beclin 1 complex and its upstream pathway that thereby led to cell death. We uncovered a novel function for the antiapoptotic<br />
protein Bcl-2, as it played a role in negatively regulating autophagy by blocking an essential protein in the signaling<br />
pathway, Beclin 1. Furthermore, Bcl-2 was activated by c-Jun N-terminal kinase 1 (JNK 1), which is upstream <strong>of</strong> Bcl-2<br />
phosphorylation, to induce Bcl-2/Beclin 1 dissociation. Furthermore, we demonstrated a novel role for melatonin in<br />
protecting cells from autophagic cell death triggered by the Bcl-2/Beclin 1 pathway by inhibiting the activation <strong>of</strong> the JNK 1,<br />
Bcl-2 upstream pathway. This study provides information regarding the link between apoptosis and autophagy signaling, which<br />
could lead to the development <strong>of</strong> therapeutic strategies that exploit the neurotoxicity <strong>of</strong> drugs <strong>of</strong> abuse. © <strong>2010</strong> The<br />
Authors.<br />
No.882<br />
Author (s) : Nopparatjamjomras, S., Panijpan, B.<br />
Title : Emperor's crown model teaches fluidics<br />
Source : Physics Education, Volume 45, Issue 2, <strong>2010</strong>, Pages 137-138<br />
Document Type : Article<br />
Keywords : –<br />
324
<strong>Abstract</strong> : –<br />
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.883<br />
Author (s) : Norbu, Dilokwanich, S., Uyasatian, U., Saguanwong, P.<br />
Title : Municipal solid waste management in Phuntsholing city, Bhutan<br />
Source : EnvironmentAsia, Volume 3, Issue 1, January <strong>2010</strong>, Pages 111-116<br />
Document Type : Article<br />
Keywords : Bhutan; Management; Municipal solid waste; Phuntsholing city<br />
<strong>Abstract</strong> : Municipal solid waste problem is a major concern in major cities in Bhutan. Despite the lack <strong>of</strong> reliable data on both waste<br />
composition and quantity, no studies have been conducted to identify problems and alternatives to improve the current<br />
system. The study objectives are: 1) to determine solid waste composition and generation rate; and 2) to investigate current<br />
solid waste management system. Six waste samples were selected in Phuntsholing city from three designated collection<br />
spots and from three collection vehicles and analyzed for their composition. Waste generation rate was computed from<br />
waste collected by collection vehicles. The investigation was carried out through interviews with municipal authorities,<br />
existing document reviews, and field observations. The organic fraction <strong>of</strong> solid waste composition comprised about 71<br />
percent. The waste generation rate was estimated to 0.40 kg/capita.day. The current management system is inefficient, and<br />
recommendations are given to improve the current situation.<br />
No.884<br />
Author (s) : Nosten, F.<br />
Title : Waking the sleeping beauty<br />
Source : Journal <strong>of</strong> Infectious Diseases, Volume 202, Issue 9, 1 November <strong>2010</strong>, Pages 1300-1301<br />
Document Type : Note<br />
Keywords : antimalarial agent, artemether, artemisinin, artesunate; antimalarial activity, antimicrobial therapy, dormancy, drug<br />
absorption, drug exposure, drug metabolism, gametocyte, gametogenesis, host parasite interaction, human, malaria<br />
falciparum, medical information, mitochondrial genome, nonhuman, note, phenotype, Plasmodium falciparum, priority<br />
journal, reinfection; Antimalarials, Artemisinins, Humans, Malaria, Falciparum, Microbial Viability, Plasmodium<br />
falciparum, Recurrence<br />
<strong>Abstract</strong> : –<br />
No.885<br />
Author (s) : Novaprateep, B., Khompurngson, K., Poltem, D.<br />
Title : Learning the value <strong>of</strong> a function by using hypercircle inequality for data error<br />
Source : <strong>International</strong> Journal <strong>of</strong> Mathematics and Computers in Simulation, Volume 5, Issue 1, <strong>2010</strong>, Pages 1-8<br />
Document Type : Article<br />
Keywords : Convex optimization and noise data; Hypercircle inequality; Regularization; Reproducing kernel hilbert space<br />
<strong>Abstract</strong> : In this paper, we briefly review Hypercircle inequality for data error (Hide) measured with square loss. We provide it in the<br />
case that the unit ball B is replaced by δB where δ is any positive number. Moreover, we also discuss some important facts<br />
<strong>of</strong> Hide for practical computation and study the problem in learning the value <strong>of</strong> a function in reproducing kernel Hilbert<br />
space (RKHS) by using the available material from Hide with different values <strong>of</strong> δ We compare our numerical experiment to<br />
the method <strong>of</strong> regularization, which is the standard method for learning problem.<br />
No.886<br />
Author (s) : Nuchprayoon, S.; Sungpradit, S.; Jedlicka, A. E.; Bailey, J.; Scott, A. L.<br />
Title : Blood micr<strong>of</strong>ilarial-stage specific gene expression pr<strong>of</strong>ile <strong>of</strong> Brugia malayi<br />
Source : INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, Volume: 14, Supplement: 1, Pages: E111-E111, DOI:<br />
10.1016/j.ijid.<strong>2010</strong>.02.1733, Published: MAR <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
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No.887<br />
Author (s) : Nugultham, K., Shiowatana, J.<br />
Title : Teaching science in Thai secondary schools using experimental kits<br />
Source : <strong>International</strong> Journal <strong>of</strong> Learning, Volume 16, Issue 12, <strong>2010</strong>, Pages 307-316<br />
Document Type : Article<br />
Keywords : Experimental kit; Hands-on; Inquiry-based learning; Test kit<br />
<strong>Abstract</strong> : This study was to develop low-cost experimental kits and to design learning tools for Thai secondary school science teaching.<br />
Test kits provide a rapid testing device. The test kits used in the study were based on colorimetric analyses. The experimental<br />
kit was designed to support students exploring and learning issues associated with substances, solution and the properties <strong>of</strong><br />
consumer products and the environment. It consisted <strong>of</strong> instructional manuals for teacher and student activities, lesson<br />
plans, assessment items in addition to the test kits for pH, phosphate and dissolved oxygen provided to help students'<br />
learning effectively. Pre-knowledge and post tests were conducted before and after using the experimental kit. The data were<br />
analyzed both in quantitative and qualitative views. Findings showed that the experimental kits could increase understanding<br />
<strong>of</strong> chemistry concepts and students enjoyed using the experimental kits while learning essential concepts and making more<br />
discussion in science. © Common Ground, Kulthida Nugultham, Juwadee Shiowatana.<br />
No.888<br />
Author (s) : Nuinoon, M., Makarasara, W., Mushiroda, T., Setianingsih, I., Wahidiyat, P.A., Sripichai, O., Kumasaka, N., Takahashi, A., Svasti, S.,<br />
Munkongdee, T., Mahasirimongkol, S., Peerapittayamongkol, C., Viprakasit, V., Kamatani, N., Winichagoon, P., Kubo, M.,<br />
Nakamura, Y., Fucharoen, S.<br />
Title : A genome-wide association identified the common genetic variants influence disease severity in β0-thalassemia/hemoglobin<br />
e<br />
Source : Human Genetics, Volume 127, Issue 3, <strong>2010</strong>, Pages 303-314<br />
Document Type : Article<br />
Keywords : alpha globin, beta globin, DNA, guanine nucleotide binding protein, hemoglobin E, protein bcl 11a, protein Myb, transcription<br />
factor, unclassified drug; adolescent, adult, article, beta thalassemia, blood transfusion, chromosome 2p, chromosome<br />
6q, controlled study, disease severity, DNA extraction, DNA microarray, dot hybridization, female, gene cluster, gene<br />
locus, gene replication, genetic association, genetic variability, genotype, heredity, human, human cell, Indonesia, major<br />
clinical study, male, phenotype, polymerase chain reaction, priority journal, restriction fragment length polymorphism, scoring<br />
system, spleen size, splenectomy; Adolescent, Adult, alpha-Globins, beta-Globins, beta-Thalassemia, Child, Child,<br />
Preschool, DNA Mutational Analysis, Female, Gene Frequency, Genome-Wide Association Study, Hemoglobin<br />
E, Humans, Male, Phenotype, Polymorphism, Single Nucleotide, Severity <strong>of</strong> Illness Index, Young Adult<br />
<strong>Abstract</strong> : β-Thalassemia/HbE disease is clinically variable. In searching for genetic factors modifying the disease severity, patients were<br />
selected based on their disease severities, and a genome-wide association study (GWAS) was performed. Genotyping was<br />
conducted with the Illumina Human 610-Quad BeadChips array using DNAs from 618 Thai β0-thalassemia/HbE patients who<br />
were classified as 383 severe and 235 mild phenotypes by a validated scoring system. Twenty-three SNPs in three<br />
independent genes/regions were identified as being significantly associated with the disease severity. The highest association<br />
was observed with SNPs in the β-globin gene cluster (chr.11p15), and rs2071348 <strong>of</strong> the HBBP1 gene revealed the most<br />
significant association [P = 2.96 × 10-13, odds ratio (OR) = 4.33 (95% confidence interval (CI), 2.74-6.84)]. The second was<br />
identified in the intergenic region between the HBS1L and MYB genes (chr.6q23), among which rs9376092 was the most<br />
significant [P = 2.36 × 10-10, OR = 3.07 (95% CI, 2.16-4.38)]. The third region was located in the BCL11A gene (chr.2p16.1), and<br />
rs766432 showed the most significant association [P = 5.87 × 10-10, OR = 3.06 (95% CI, 2.15-4.37)]. All three loci were<br />
replicated in an independent cohort <strong>of</strong> 174 Indonesian patients. The associations to fetal hemoglobin levels were also<br />
observed with SNPs on these three regions. Our data indicate that several genetic loci act in concert to influence HbF levels<br />
<strong>of</strong> β0-thalassemia/HbE patients. This study revealed that all the three reported loci and the α-globin gene locus are the best<br />
and common predictors <strong>of</strong> the disease severity in β-thalassemia. © 2009 Springer-Verlag.<br />
No.889<br />
Author (s) : Numbenjapon N., Nakavachara P., Santiprabhob J., Kiattisakthavee P., Wongarn R., Likitmaskul S.<br />
Title : Successful strategy to improve glucose tolerance in Thai obese youth.<br />
Source : J Med Assoc Thai, <strong>2010</strong>; 93 (Suppl. 6): S131-S138<br />
Document Type : Article<br />
326
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : Obesity, Impaired glucose tolerance, Diabetes mellitus, Lifestyle modification, Metformin, Children, Adolescents<br />
<strong>Abstract</strong> : BACKGROUND: Childhood obesity is an emerging national health problem in Thailand. Our previous study found that one<br />
third <strong>of</strong> obese children and adolescents had impaired glucose tolerance (IGT) and 2.6 percent had already developed type 2<br />
diabetes mellitus. An immediate strategy needs to be established in order to improve these metabolic problems. OBJECTIVE:<br />
To determine whether diet and exercise education for lifestyle modification with or without metformin therapy in our<br />
diabetes clinic is enable to improve these metabolic problems. MATERIAL AND METHOD: Twenty-six Thai obese children and<br />
adolescents with IGT, who received at least 6 months <strong>of</strong> treatment consisting <strong>of</strong> lifestyle modification alone or lifestyle<br />
modification and metformin (combined treatment) were enrolled into this study. Each patient underwent the second 2-hour<br />
oral glucose tolerance test (OGTT). Plasma glucose, insulin levels, HbA1C and lipid pr<strong>of</strong>iles were measured. The results were<br />
compared with historical pre-treatment data. RESULTS: Approximately 1 year after intervention, 19 out <strong>of</strong> 26 patients with IGT<br />
completed the second 2-hour OGTT. Sixteen patients (84.2%) successfully reversed to be normal glucose tolerance whereas<br />
3 patients (15.8%) remained IGT. Body mass index (BMI), BMISDS, 2-hour plasma glucose, basal insulin level, 2-hour insulin<br />
level were significantly decreased after treatment in normal OGTT group (Ps < 0.05). Treatment with lifestyle modification<br />
alone and combined treatment indifferently improved the abnormal glucose tolerance in our patient (83.3% vs. 84.6%).<br />
CONCLUSION: Impaired glucose tolerance in obese youth is a reversible abnormality by lifestyle modification with or without<br />
metformin.<br />
No.890<br />
Author (s) : Nunta-Aree, S., Sitthinamsuwan, B., Boonyapisit, K., Pisarnpong, A.<br />
Title : Sw2-year outcomes <strong>of</strong> subthalamic deep brain stimulation for idiopathic Parkinson's disease<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 5, May <strong>2010</strong>, Pages 529-540<br />
Document Type : Article<br />
Keywords : Deep brain stimulation; Parkinson's disease; Subthalamic nucleus<br />
<strong>Abstract</strong> : Background: Deep brain stimulation <strong>of</strong> the subthalamic nucleus (STN-DBS) is the recent surgical treatment <strong>of</strong> choice for<br />
patients with idiopathic Parkinson's disease (PD) complicated by motor fluctuation and disabling dyskinesia. Objective: To<br />
study 2 years clinical outcomes, changes <strong>of</strong> medication and complications following STN-DBS in patients with advanced PD.<br />
Material and Method: Twenty-seven patients with 2-year follow-up and complete data were enrolled for retrospective<br />
evaluation <strong>of</strong> Unified Parkinson's Disease Rating Scale (UPDRS) and levodopa equivalent dose (LED). Postoperative UPDRS at 6month,<br />
1-year and 2-years were compared with the preoperative corresponding UPDRS. Postoperative LED at 2 years was<br />
compared with the preoperative baseline. Statistical analysis was performed with paired t-test. Additionally, 62 patients with<br />
STN-DBS were enrolled for evaluation <strong>of</strong> treatment complications. Results: Of 27 patients with complete 2-years follow-up,<br />
preoperative dopamine challenge test showed 50.6% improvement <strong>of</strong> motor score (UPDRS axis III). Mentation, behavior and<br />
mood (UPDRS axis I) were not significantly improved in each subscore, but significantly improved in the total score. Marked<br />
improvement <strong>of</strong> activities <strong>of</strong> daily living (UPDRS axis II) and complications <strong>of</strong> therapy (UPDRS IV) was found. Two-year<br />
postoperative motor score (UPDRS axis III) during "<strong>of</strong>f medication-on stimulator" showed progressive and dramatic<br />
improvement by mean <strong>of</strong> 59.83%. The present study also revealed significant improvement <strong>of</strong> motor score (UPDRS axis III)<br />
during "on medication-on stimulator" in some items. A significant 33.4% reduction <strong>of</strong> LED was noted. Of 62 patients with<br />
bilateral STN-DBS, there was 1 asymptomatic intracerebral hemorrhage (0.8% per side), 2 speech difficulty (3.2%), 1 transient<br />
confusion (1.6%), 2 transient hypomania (3.2%), 1 stimulation induced hemiballism (1.6%), 1 wound infection (1.6%) and 1<br />
lead malposition (0.8% per side). Conclusion STN-DBS is a safe and effective treatment for PD complicated by motor<br />
fluctuation or dyskinesia. The operative outcomes show long-term improvement <strong>of</strong> activities <strong>of</strong> daily living, motor function<br />
and reduction <strong>of</strong> medication and drugrelated complications.<br />
No.891<br />
Author (s) : Nunta-Aree, S., Tuntongtip, D., Sitthinamsuwan, B., Srirabheebhat, P., Nitising, A., Witthiwej, T.<br />
Title : Accuracy <strong>of</strong> various subthalamic nucleus targeting methods and an appropriated formula for Thai patients<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 9, September <strong>2010</strong>, Pages 1043-1049<br />
Document Type : Article<br />
Keywords : Deep brain stimulation; Subthalamic nucleus; Targeting method<br />
<strong>Abstract</strong> : Objective: Deep brain stimulation (DBS) <strong>of</strong> the subthalamic nucleus (STN) has recently been the standard surgical treatment<br />
for Parkinson's disease. Besides appropriate selection <strong>of</strong> patients, precise STN targeting is the most crucial factor for good<br />
surgical outcomes. However, there is no single targeting method universally accepted as the most accurate technique. Thus,<br />
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the authors studied the accuracy <strong>of</strong> various STN targeting methods and proposed a formula for Thai patients. Material and<br />
Method: Sixteen patients with Parkinson's disease who fulfilled the intraoperative criteria <strong>of</strong> good STN targeting and had<br />
significant clinical improvements, without any stimulation-induced adverse effects, were included in the present study.<br />
Positions <strong>of</strong> the STN were determined by four targeting methods, direct targeting by axial T2W MRI, direct targeting by coronal<br />
T2W MRI, indirect targeting by anterior commissure-posterior commissure based formula and indirect targeting by adjustable<br />
digital Schaltenbrand-Wahren (SW) atlas, were compared with the final lead positions. The final lead positions by the four<br />
targeting methods were averaged to get an appropriate AC-PC based formula for Thai patients. Results: The most accurate<br />
STN targeting method was axial T2W MRI followed by coronal T2W MRI, AC-PC based formula and the SW atlas, respectively.<br />
The averaged final lead positions obtained from the four methods was X = 11.5 ± 1.0, Y = -3.3 ± 1.0 and Z = -4.8 ± 0.42 mm.<br />
Conclusion: The direct targeting by axial T2W MRI yielded the highest accuracy and the appropriate STN formula for Thai<br />
patients appeared to be X = ±11.5, Y = -3.5 (-3.3) and Z = -5.0 (-4.8) mm.<br />
No.892<br />
Author (s) : Nuntagowat, C., Leelawat, K., Tohtong, R.<br />
Title : NGAL knockdown by siRNA in human cholangiocarcinoma cells suppressed invasion by reducing NGAL/MMP-9 complex<br />
formation<br />
Source : Clinical and Experimental Metastasis, Volume 27, Issue 5, May <strong>2010</strong>, Pages 295-305<br />
Document Type : Article<br />
Keywords : Cholangiocarcinoma; Invasion; MMP-9; NGAL; siRNA<br />
<strong>Abstract</strong> : We studied the role <strong>of</strong> Neutrophil Gelatinase-associated Lipocalin (NGAL, lipocalin 2) in regulating the invasiveness <strong>of</strong> a<br />
cholangiocarcinoma cell line, RMCCA-1. RMCCA-1 cells expressed multiple forms <strong>of</strong> 25, 40, 75 and 115/135 kDa NGAL which<br />
were detected in the conditioned medium, whereas only the 25 kDa form was detected in the cell lysates. NGAL expression<br />
was induced by serum deprivation. NGAL downregulation by siRNA suppressed NGAL mRNA and protein expression by about<br />
70-80%, concommittant with a significant reduction <strong>of</strong> in vitro invasiveness, migration and pro-MMP-9 activity, but not cell<br />
proliferation. Suppression <strong>of</strong> pro-MMP-9 activity paralleled a reduction <strong>of</strong> NGAL/MMP-9 complex in the conditioned medium,<br />
although MMP-9 mRNA expression was unaffected. Our data suggest that NGAL promotes the invasiveness <strong>of</strong> the<br />
cholangiocarcinoma cells by forming complex with MMP-9, stabilizing its activity and rendering the cancer cells to be more<br />
invasive. © <strong>2010</strong> Springer Science+Business Media B.V.<br />
No.893<br />
Author (s) : Nuntapornsak, A., Wongdee, K., Thongbunchoo, J., Krishnamra, N., Charoenphandhu, N.<br />
Title : Changes in the mRNA expression <strong>of</strong> osteoblast-related genes in response to β3-adrenergic agonist in UMR106 cells<br />
Source : Cell Biochemistry and Function, Volume 28, Issue 1, January <strong>2010</strong>, Pages 45-51<br />
Document Type : Article<br />
Keywords : Adrenergic receptor; Alkaline phosphatase; Bone remodeling; Osteocalcin; Osteoprotegerin; Real-time PCR<br />
<strong>Abstract</strong> : Activation <strong>of</strong> adrenergic receptors (AR) was demonstrated to result in either bone gain or bone loss depending on the<br />
activated AR subtypes and concentrations <strong>of</strong> agonists used. While β2-AR agonist was extensively investigated as an<br />
osteopenic agent, effects <strong>of</strong> β3-AR activation on osteoblasts were still elusive. Rat osteoblast-like UMR106 cells were herein<br />
found to express several AR subtypes, including β3-AR. After exposure to a low-dose β3-AR agonist BRL37344 (10 nmol L-1),<br />
UMR106 cells downregulated the mRNA expression <strong>of</strong> transcription factors Runx2 and Dlx5, which are important for initiation<br />
<strong>of</strong> osteoblast differentiation. Low-dose BRL37344 also decreased the expression ratio <strong>of</strong> receptor activator <strong>of</strong> nuclear factor<br />
kB ligand (RANKL) over osteoprotegerin (OPG), suggesting the protective effect <strong>of</strong> β3-AR agonist against bone resorption.<br />
Alkaline phosphatase expression was markedly decreased, whereas expressions <strong>of</strong> osteocalcin and osteopontin were<br />
increased by 100 nmol L-1 BRL37344, indicating that β3-AR activation could accelerate the transition <strong>of</strong> matrix maturation<br />
stage to mineralization stage. In conclusion, b3-AR activation in rat osteoblasts induced alteration in the expression <strong>of</strong><br />
osteoblast-related transcription factor genes as well as genes required for bone formation and resorption. The present results<br />
also suggest that, besides β2-AR, β3-AR is another AR subtype responsible for the sympathetic nervous system-induced bone<br />
remodeling. Copyright © 2009 John Wiley & Sons, Ltd.<br />
No.894<br />
Author (s) : Nuntasukkasame, N.<br />
Title : PRESSURE ULCER PREVENTION AND CURE OF PRESSURE ULCER INPATIENTS IN SIRIRAJ HOSPITAL (THAILAND)<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Source : JOURNAL OF WOUND OSTOMY AND CONTINENCE NURSING, Volume: 37, Issue: 3, Supplement: S, Pages: S104-S105, Published:<br />
MAY-JUN <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.895<br />
Author (s) : Nuntnarumit, P., Rojnueangnit, K., Tangnoo, A.<br />
Title : Oxygen saturation trends in preterm infants during the first 15 min after birth<br />
Source : Journal <strong>of</strong> Perinatology, Volume 30, Issue 6, June <strong>2010</strong>, Pages 399-402<br />
Document Type : Article<br />
Keywords : After birth; Oxygen saturation; Preterm infants<br />
<strong>Abstract</strong> : Objective:To determine oxygen saturation (SpO2) trends in healthy preterm newborns during the first 15 min after birth and<br />
to ascertain factors affecting SpO 2 changes during that period.Study Design:An observational study was conducted. Preterm<br />
newborns with gestational age (GA) less than 35 weeks, who did not require oxygen supplementation during the first 15 min<br />
<strong>of</strong> life, were enrolled. Pulse oximetry was applied to the right hand immediately after birth. SpO2 data were recorded<br />
continuously every 2 s and were then transferred to a Micros<strong>of</strong>t Excel spreadsheet for analysis. The time to reach a stable<br />
SpO2 85 ≥or ≥90% was described by the Kaplan-Meier method and compared using log-rank test.Result:A total <strong>of</strong> 102<br />
preterm newborns were eligible, <strong>of</strong> whom 27 were excluded, resulting in 75 newborns enrolled into the study with a median<br />
(range) GA <strong>of</strong> 35 (29 to 35) weeks, median (range) birth weight <strong>of</strong> 2390 (1270 to 2990) g and median (range) Apgar scores at 1<br />
and 5 min <strong>of</strong> 9 (5 to 10) and 10 (7 to 10), respectively. There was a gradual increase in SpO 2 with time. The median<br />
(interquartile range, IQR) SpO 2 at 2, 3, 4, 5 and 6 min was 77% (72 to 92), 84% (75 to 94), 88% (80 to 94), 90% (79 to 95) and<br />
95% (85 to 97), respectively. Newborns with a lower GA seemed to have lower SpO 2 values. The median (IQR) time to reach<br />
a stable SpO2 ≥85 or ≥90% was 4 (3 to 6) and 5 (3 to 57) min, respectively. The mode <strong>of</strong> delivery was a significant factor<br />
affecting SpO 2; infants delivered by cesarean section had a significantly lower SpO2 and took a longer time to reach SpO2<br />
≥85% compared with those delivered by vaginal delivery (HR1.96, 95% CI1.11 to 3.49; P0.02). All enrolled newborns had no<br />
serious complications and they survived to discharge.Conclusion:SpO 2 in preterm newborns gradually increased with time.<br />
Approximately half <strong>of</strong> the preterm newborns had an SpO2 <90% during the first 5 min <strong>of</strong> life. Infants delivered by cesarean<br />
section had a lower SpO2 than those delivered by vaginal delivery. © <strong>2010</strong> Nature Publishing Group All rights reserved.<br />
No.896<br />
Author (s) : Nuurai, P., Engsusophon, A., Poomtong, T., Sretarugsa, P., Hanna, P., Sobhon, P., Wanichanon, C.<br />
Title : Stimulatory effects <strong>of</strong> egg-laying hormone and gonadotropin-releasing hormone on reproduction <strong>of</strong> the tropical abalone,<br />
Haliotis asinina Linnaeus<br />
Source : Journal <strong>of</strong> Shellfish Research, Volume 29, Issue 3, November <strong>2010</strong>, Pages 627-635<br />
Document Type : Conference Paper<br />
Keywords : abalone; egg laying hormone; gonad maturation; gonadotropin releasing hormone; Haliotis asinina; sexual<br />
differentiation; spawning<br />
<strong>Abstract</strong> : Egg-laying hormone (ELH) is a neuropeptide hormone that stimulates ovulation <strong>of</strong> gastropods, including Aplysia californica and<br />
Lymnaea stagnalis. Other neuropeptides, gonadotropin releasing hormones (GnRHs), also play important roles in controlling<br />
reproduction in both vertebrates and invertebrates. In the current study, the effects <strong>of</strong> abalone ELH (aELH) and several GnRHs<br />
on somatic growth, sex differentiation, gonad maturation, and spawning <strong>of</strong> Haliotis asinina were investigated in 3 experiments.<br />
In experiment 1, groups <strong>of</strong> 4-mo-old juveniles (11.8 ± 0.03 mm shell length (SL) and 0.33 ± 0.04 g body weight (BW)) were<br />
injected with aELH and GnRHs, including buserelin (mammalian GnRH analogue), octopus GnRH (octGnRH), and tunicate GnRH-<br />
I (tGnRH-I), at doses <strong>of</strong> 20 ng/g BW and 200 ng/g BW. The aELH induced early sex differentiation with a bias toward females,<br />
but with normal somatic growth, whereas the different is<strong>of</strong>orms <strong>of</strong> GnRH had no effect on sexual differentiation or somatic<br />
growth. In experiment 2, groups <strong>of</strong> 1-y-old-abalone (SL, 4.04 ± 0.02 cm; BW, 20.15 ± 0.25 g) were injected with aELH and the 3<br />
is<strong>of</strong>orms <strong>of</strong> GnRH including buserelin, octGnRH, and lamprey GnRH (IGnRH-I) at doses <strong>of</strong> 500 ng/g BW and 1,000 ng/g BW, and<br />
all produced stimulatory effects. For each peptide treatment, the gonads reached full maturation within 56 wk and spawning<br />
occurred, whereas control groups took 8 wk to reach maturity. In experiment 3, injections <strong>of</strong> ripe abalone with aELH<br />
stimulated spawning <strong>of</strong> both sexes in a dose-dependent manner. Buserelin had a lesser effect on inducing spawning, and<br />
octGnRH had no apparent effect. The gametes released from induced spawnings by aELH and GnRH showed normal<br />
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fertilization and development <strong>of</strong> larvae. Altogether, these findings provide further knowledge on manipulating abalone<br />
reproduction, which is important in improving abalone aquaculture.<br />
No.897<br />
Author (s) : Nuurai, P., Poljaroen, J., Tinikul, Y., Cummins, S., Sretarugsa, P., Hanna, P., Wanichanon, C., Sobhon, P.<br />
Title : The existence <strong>of</strong> gonadotropin-releasing hormone-like peptides in the neural ganglia and ovary <strong>of</strong> the abalone, Haliotis asinina<br />
L.<br />
Source : Acta Histochemica, Volume 112, Issue 6, November <strong>2010</strong>, Pages 557-566<br />
Document Type : Article<br />
Keywords : Abalone; Gnrh; Gonadotropin-releasing hormone; Haliotis asinina; Immunohistochemistry; Neural ganglia; Ovary<br />
<strong>Abstract</strong> : Gonadotropin-releasing hormone (GnRH) is a neuropeptide that is conserved in both vertebrate and invertebrate species. In<br />
this study, we have demonstrated the presence and distribution <strong>of</strong> two is<strong>of</strong>orms <strong>of</strong> GnRH-like peptides in neural ganglia and<br />
ovary <strong>of</strong> reproductively mature female abalone, Haliotis asinina, using immunohistochemistry. We found significant<br />
immunoreactivities (ir) <strong>of</strong> anti-lamprey(l) GnRH-III and anti-tunicate(t) GnRH, but with variation <strong>of</strong> labeling intensity by each<br />
anti-GnRH type. lGnRH-III-ir was detected in numerous type 1 neurosecretory cells (NS1) throughout the cerebral and<br />
pleuropedal ganglia, whereas tGnRH-I-ir was detected in only a few NS1 cells in the dorsal region <strong>of</strong> cerebral and pleuropedal<br />
ganglia. In addition, a small number <strong>of</strong> type 2 neurosecretory cells (NS2) in cerebral ganglion showed lGnRH-III-ir. Long nerve<br />
fibers in the neuropil <strong>of</strong> ventral regions <strong>of</strong> the cerebral and pluropedal ganglia showed strong tGnRH-I-ir. In the ovary, lGnRH-<br />
III-ir was found primarily in oogonia and stage I oocytes, whereas tGnRH-ir was observed in stage I oocytes and some stage II<br />
oocytes. These results indicate that GnRH produced in neural ganglia may act in neural signaling. Alternatively, GnRH may also<br />
be synthesized locally in the ovary where it could induce oocyte development. © 2009 Elsevier GmbH.<br />
No.898<br />
Author (s) : Nyunt, S.W., Howteerakul, N., Suwannapong, N., Rajatanun, T.<br />
Title : Self-efficacy, self-care behaviors and glycemic control among type-2 diabetes patients attending two private clinics in Yangon,<br />
Myanmar<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 4, July <strong>2010</strong>, Pages 943-951<br />
Document Type : Article<br />
Keywords : Diabetes; Glycemic control; Myanmar; Self efficacy; Self-care behavior<br />
<strong>Abstract</strong> : This cross-sectional study aimed to estimate the prevalence <strong>of</strong> glycemic control and its associated factors among type-2<br />
diabetes patients attending two private clinics in Yangon, Myanmar. Two hundred sixty-six diabetes patients attending two<br />
private diabetes clinics in Yangon during February and March, 2009 were included in the study. The participants completed a<br />
structured questionnaire. HbA1c was used as the index for glycemic control. The prevalence <strong>of</strong> successful glycemic control<br />
(HbA1c≤7%) was 27.1%. The median HbA1c value was 7.8%. About 62.0% <strong>of</strong> patients had high self-efficacy levels, and 30.8%<br />
had good self-care behavior. Multiple logistic regression analysis revealed four variables associated with glycemic control: age<br />
≥60 years (OR 2.46, 95%CI 1.17-5.21), taking one oral hypoglycemic agent (OHA) (OR 2.56, 95%CI 1.26-5.19), being overweight<br />
(OR 2.01, 95%CI 1.02-3.95) and having a high self-efficacy level (OR 5.29, 95%CI 2.20-12.75). Interventions to increase diabetic<br />
patient self-efficacy levels and selfcare behavior, especially related to diet and exercise, are needed to reduce poor glycemic<br />
control.<br />
No.899<br />
Author (s) : Okamoto, M., Nakao, M., Blair, D., Anantaphruti, M.T., Waikagul, J., Ito, A.<br />
Title : Evidence <strong>of</strong> hybridization between Taenia saginata and Taenia asiatica<br />
Source : Parasitology <strong>International</strong>, Volume 59, Issue 1, March <strong>2010</strong>, Pages 70-74<br />
Document Type : Article<br />
Keywords : Hybridization; Outcrossing; Taenia asiatica; Taenia saginata<br />
<strong>Abstract</strong> : There has long been a debate as to the specific status <strong>of</strong> the cestode Taenia asiatica, with some people regarding it as a<br />
distinct species and some preferring to recognize it as a strain <strong>of</strong> Taenia saginata. The balance <strong>of</strong> current opinion seems to be<br />
that T. asiatica is a distinct species. In this study we performed an allelic analysis to explore the possibility <strong>of</strong> gene exchange<br />
between these closely related taxa. In total, 38 taeniid tapeworms were collected from humans living in many localities<br />
including Kanchanaburi Province, Thailand where the two species are sympatric. A mitochondrial DNA (mtDNA)-based<br />
multiplex PCR tentatively identified those parasites as T. asiatica (n = 20) and T. saginata (n = 18). Phylogenetic analyses <strong>of</strong> a<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
mitochondrial cytochrome c oxidase subunit 1 (cox1) gene and two nuclear loci, for elongation factor-1 alpha (ef1) and ezrinradixin-moesin<br />
(ERM)-like protein (elp), assigned all except two individual parasites to the species indicated by multiplex PCR.<br />
The two exceptional individuals, from Kanchanaburi Province, showed a discrepancy between the mtDNA and nuclear DNA<br />
phylogenies. In spite <strong>of</strong> their possession <strong>of</strong> sequences typical <strong>of</strong> the T. saginata cox1 gene, both were homozygous at the elp<br />
locus for one <strong>of</strong> the alleles found in T. asiatica. At the ef1 locus, one individual was homozygous for the allele found at high<br />
frequency in T. asiatica while the other was homozygous for the major allele in T. saginata. These findings are evidence <strong>of</strong><br />
occasional hybridization between the two species, although the possibility <strong>of</strong> retention <strong>of</strong> ancestral polymorphism cannot be<br />
excluded. © 2009 Elsevier Ireland Ltd. All rights reserved.<br />
No.900<br />
Author (s) : Okiro, E.A., White, L.J., Ngama, M., Cane, P.A., Medley, G.F., Nokes, D.J.<br />
Title : Duration <strong>of</strong> shedding <strong>of</strong> respiratory syncytial virus in a community study <strong>of</strong> Kenyan children<br />
Source : BMC Infectious Diseases, Volume 10, 22 January <strong>2010</strong>, Article number 15<br />
Document Type : Article<br />
Keywords : adolescent, age, article, child, controlled study, disease duration, disease severity, female, human, immun<strong>of</strong>luorescence<br />
test, infant, Kenya, lower respiratory tract infection, major clinical study, male, newborn, preschool child, prognosis, public<br />
health, reinfection, Respiratory syncytial pneumovirus, school child, sex difference, viral upper respiratory tract infection, virus<br />
infection, virus shedding, virus transmission, physiology, proportional hazards model, respiratory syncytial virus<br />
infection, virology; Adolescent, Child, Child, Preschool, Female, Humans, Infant, Kenya, Male, Proportional Hazards<br />
Models, Respiratory Syncytial Virus Infections, Respiratory Syncytial Viruses, Virus Shedding<br />
<strong>Abstract</strong> : Background: Our understanding <strong>of</strong> the transmission dynamics <strong>of</strong> respiratory syncytial virus (RSV) infection will be better<br />
informed with improved data on the patterns <strong>of</strong> shedding in cases not limited only to hospital admissions.Methods: In a<br />
household study, children testing RSV positive by direct immun<strong>of</strong>luorescent antibody test (DFA) were enrolled. Nasal<br />
washings were scheduled right away, then every three days until day 14, every 7 days until day 28 and every 2 weeks until a<br />
maximum <strong>of</strong> 16 weeks, or until the first DFA negative RSV specimen. The relationship between host factors, illness severity<br />
and viral shedding was investigated using Cox regression methods.Results: From 151 families a total <strong>of</strong> 193 children were<br />
enrolled with a median age <strong>of</strong> 21 months (range 1-164 months), 10% infants and 46% male. The rate <strong>of</strong> recovery from<br />
infection was 0.22/person/day (95% CI 0.19-0.25) equivalent to a mean duration <strong>of</strong> shedding <strong>of</strong> 4.5 days (95%CI 4.0-5.3), with<br />
a median duration <strong>of</strong> shedding <strong>of</strong> 4 days (IQR 2-6, range 1-14). Children with a history <strong>of</strong> RSV infection had a 40% increased<br />
rate <strong>of</strong> recovery i.e. shorter duration <strong>of</strong> viral shedding (hazard ratio 1.4, 95% CI 1.01-1.86). The rate <strong>of</strong> cessation <strong>of</strong> shedding<br />
did not differ significantly between males and females, by severity <strong>of</strong> infection or by age.Conclusion: We provide evidence <strong>of</strong><br />
a relationship between the duration <strong>of</strong> shedding and history <strong>of</strong> infection, which may have a bearing on the relative role <strong>of</strong><br />
primary versus re-infections in RSV transmission in the community. © <strong>2010</strong> Okiro et al; licensee BioMed Central Ltd.<br />
No.901<br />
Author (s) : Olsen, S.J., Supawat, K., Campbell, A.P., Anantapreecha, S., Liamsuwan, S., Tunlayadechanont, S., Visudtibhan, A.,<br />
Lupthikulthum, S., Dhiravibulya, K., Viriyavejakul, A., Vasiknanonte, P., Rajborirug, K., Watanaveeradej, V., Nabangchang, C.,<br />
Laven, J., Kosoy, O., Panella, A., Ellis, C., Henchaichon, S., Khetsuriani, N., Powers, A.M., Dowell, S.F., Fischer, M.<br />
Title : Japanese encephalitis virus remains an important cause <strong>of</strong> encephalitis in Thailand<br />
Source : <strong>International</strong> Journal <strong>of</strong> Infectious Diseases, Volume 14, Issue 10, October <strong>2010</strong>, Pages e888-e892<br />
Document Type : Article<br />
Keywords : Encephalitis; Japanese encephalitis; Surveillance; Vaccine<br />
<strong>Abstract</strong> : Background: Japanese encephalitis virus (JEV) is endemic in Thailand and prevention strategies include vaccination, vector<br />
control, and health education. Methods: Between July 2003 and August 2005, we conducted hospital-based surveillance for<br />
encephalitis at seven hospitals in Bangkok and Hat Yai. Serum and cerebrospinal (CSF) specimens were tested for evidence <strong>of</strong><br />
recent JEV infection by immunoglobulin M (IgM) enzyme-linked immunosorbent assay (ELISA) and a plaque reduction<br />
neutralization test (PRNT). Results: Of the 147 patients enrolled and tested, 24 (16%) had evidence <strong>of</strong> acute flavivirus<br />
infection: 22 (15%) with JEV and two (1%) with dengue virus. Of the 22 Japanese encephalitis (JE) cases, 10 (46%) were aged<br />
≤15 years. The median length <strong>of</strong> hospital stay was 13 days; one 13-year-old child died. Ten percent <strong>of</strong> encephalitis patients<br />
enrolled in Bangkok hospitals were found to have JEV infection compared to 28% <strong>of</strong> patients enrolled in hospitals in<br />
southern Thailand (p< 0.01). Four (40%) <strong>of</strong> the 10 children with JE were reported as being vaccinated. Conclusions: JEV<br />
remains an important cause <strong>of</strong> encephalitis among hospitalized patients in Thailand. The high proportion <strong>of</strong> JE among<br />
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encephalitis cases is concerning and additional public health prevention efforts or expanded vaccination may be needed. ©<br />
<strong>2010</strong>.<br />
No.902<br />
Author (s) : Omodeo-Salè, F., Cortelezzi, L., Vommaro, Z., Scaccabarozzi, D., Dondorp, A.M.<br />
Title : Dysregulation <strong>of</strong> L-arginine metabolism and bioavailability associated to free plasma heme<br />
Source : American Journal <strong>of</strong> Physiology - Cell Physiology, Volume 299, Issue 1, July <strong>2010</strong>, Pages C148-C154<br />
Document Type : Article<br />
Keywords : Arginase; Ferriprotoporphyrin IX; Malaria<br />
<strong>Abstract</strong> : Severe Plasmodium falciparum malaria is associated with hypoargininemia, which contributes to impaired systemic and<br />
pulmonary nitric oxide (NO) production and endothelial dysfunction. Since intravascular hemolysis is an intrinsic feature <strong>of</strong><br />
severe malaria, we investigated whether and by which mechanisms free heme [Fe(III)-protoporphyrin IX (FP)] might contribute<br />
to the dysregulation <strong>of</strong> L-arginine (L-Arg) metabolism and bioavailability. Carrier systems "y+" [or cationic amino acid<br />
transporter (CAT)] and "y+L" transport L-Arg into red blood cells (RBC), where it is hydrolyzed to ornithine and urea by<br />
arginase (is<strong>of</strong>orm I) or converted to NO • and citrulline by endothelial nitric oxide synthase (eNOS). Our results show a<br />
significant and dose-dependent impairment <strong>of</strong> L-Arg transport into RBC pretreated with FP, with a strong inhibition <strong>of</strong> the<br />
system carrier y+L. Despite the impaired L-Arg influx, higher amounts <strong>of</strong> L-Arg-derived urea are produced by RBC preexposed<br />
to FP caused by activation <strong>of</strong> RBC arginase I. This activation appeared not to be mediated by oxidative modifications <strong>of</strong> the<br />
enzyme. We conclude that L-Arg transport across RBC membrane is impaired and arginase-mediated L-Arg consumption<br />
enhanced by free heme. This could contribute to reduced NO production in severe malaria. Copyright © <strong>2010</strong> the American<br />
Physiological Society.<br />
No.903<br />
Author (s) : Ongvarrasopone, C., Chomchay, E., Panyim, S.<br />
Title : Antiviral effect <strong>of</strong> PmRab7 knock-down on inhibition <strong>of</strong> Laem-Singh virus replication in black tiger shrimp<br />
Source : Antiviral Research, Volume 88, Issue 1, October <strong>2010</strong>, Pages 116-118<br />
Document Type : Article<br />
Keywords : Double-stranded RNA; Penaeus monodon; Rab7; RNAi<br />
<strong>Abstract</strong> : PmRab7 is a Penaeus monodon small GTPase protein possibly involved in replication <strong>of</strong> several shrimp viruses. In this study<br />
RNA interference (RNAi) using double-stranded RNA (dsRNA) targeting PmRab7 gene (dsRNA-PmRab7) was employed to silence<br />
the expression <strong>of</strong> PmRab7 to investigate the inhibitory effect on Laem-Singh virus (LSNV) replication. Injection <strong>of</strong> dsRNA-<br />
PmRab7 24. h before challenge with the virus resulted in a drastic decrease <strong>of</strong> PmRab7 mRNA and complete inhibition <strong>of</strong><br />
LSNV replication at 3 days post-challenge. In a therapeutic mode, shrimp injected with dsRNA-PmRab7 1 day but not at 3 or 5<br />
days post-LSNV challenge resulted in inhibition <strong>of</strong> LSNV replication. These results pave the way to use dsRNA-PmRab7 to<br />
prevent or cure LSNV infection in shrimp. © <strong>2010</strong> Elsevier B.V.<br />
No.904<br />
Author (s) : Onlamoon, N., Noisakran, S., Hsiao, H.-M., Duncan, A., Villinger, F., Ansari, A.A., Perng, G.C.<br />
Title : Dengue virus - Induced hemorrhage in a nonhuman primate model<br />
Source : Blood, Volume 115, Issue 9, 4 March <strong>2010</strong>, Pages 1823-1834<br />
Document Type : Article<br />
Keywords : creatine kinase, D dimer, hemoglobin; animal experiment, animal model, animal tissue, article, B lymphocyte, bleeding, blood<br />
chemistry, blood component, concentration (parameters), controlled study, Dengue<br />
virus, female, hematocrit, Macaca, male, monocyte, neutropenia, nonhuman, pathogenesis, primate, priority journal, T<br />
lymphocyte, thrombocyte, thrombocyte aggregation, thrombocytopenia, viremia, virus infection, virus<br />
strain, animal, blood, dengue, disease model, human, leukocyte, pathogenicity, pathology, time, virology, virus load;<br />
Animals, Dengue Hemorrhagic Fever, Dengue Virus, Disease Models, Animal, Female, Humans, Leukocytes, Macaca<br />
mulatta, Male, Platelet Aggregation, Time Factors, Viral Load<br />
<strong>Abstract</strong> : Lack <strong>of</strong> a dengue hemorrhagic animal model recapitulating human dengue virus infection has been a significant impediment<br />
in advancing our understanding <strong>of</strong> the early events involved in the pathogenesis <strong>of</strong> dengue disease. In efforts to address this<br />
issue, a group <strong>of</strong> rhesus macaques were intravenously infected with dengue virus serotype 2 (strain 16 681) at 1 × 107<br />
PFU/animal. A classic dengue hemorrhage developed 3 to 5 days after infection in 6 <strong>of</strong> 6 animals. Blood chemistry appeared<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
to be normal with exception <strong>of</strong> creatine phosphokinase, which peaked at 7 days after infection. A modest thrombocytopenia<br />
and noticeable neutropenia concomitant with slight decrease <strong>of</strong> hemoglobin and hematocrit were registered. In addition, the<br />
concentration <strong>of</strong> D-dimer was elevated significantly. Viremia peaked at 3 to 5 days after infection followed by an inverse<br />
relationship between T and B lymphocytes and a bimodal pattern for platelet-monocytes and platelet-neutrophil aggregates.<br />
Dengue virus containing platelets engulfed by monocytes was noted at 8 or 9 days after infection. Thus, rhesus macaques<br />
inoculated intravenously with a high dose <strong>of</strong> dengue virus produced dengue hemorrhage, which may provide a unique<br />
platform to define the early events in dengue virus infection and help identify which blood components contribute to the<br />
pathogenesis <strong>of</strong> dengue disease. © <strong>2010</strong> by The American Society <strong>of</strong> Hematology.<br />
No.905<br />
Author (s) : Ooi, C.J., Fock, K.M., Makharia, G.K., Goh, K.L., Ling, K.L., Hilmi, I., Lim, W.C., Kelvin, T., Gibson, P.R., Gearry, R.B., Ouyang, Q.,<br />
Sollano, J., Manatsathit, S., Rerknimitr, R., Wei, S.-C., Leung, W.K., De Silva, H.J., Leong, R.W.<br />
Title : The Asia-Pacific consensus on ulcerative colitis<br />
Source : Journal <strong>of</strong> Gastroenterology and Hepatology, Volume 25, Issue 3, March <strong>2010</strong>, Pages 453-468<br />
Document Type : Review<br />
Keywords : Asia Pacific; Biologic agents; Consensus statements; Delphi; Diagnosis; Epidemiology; Inflammatory bowel<br />
disease; Leukocytapheresis; Management; Ulcerative colitis<br />
<strong>Abstract</strong> : Inflammatory bowel disease (IBD) is increasing in many parts <strong>of</strong> the Asia-Pacific region. There is a need to improve the<br />
awareness <strong>of</strong> IBD and develop diagnostic and management recommendations relevant to the region. This evidence-based<br />
consensus focuses on the definition, epidemiology and management <strong>of</strong> ulcerative colitis (UC) in Asia. A multi-disciplinary<br />
group developed the consensus statements, reviewed the relevant literature, and voted on them anonymously using the<br />
Delphi method. The finalized statements were reviewed to determine the level <strong>of</strong> consensus, evidence quality and strength<br />
<strong>of</strong> recommendation. Infectious colitis must be excluded prior to diagnosing UC. Typical histology and macroscopic extent <strong>of</strong><br />
the disease seen in the West is found in the Asia-Pacific region. Ulcerative colitis is increasing in many parts <strong>of</strong> Asia with<br />
gender distribution and age <strong>of</strong> diagnosis similar to the West. Extra-intestinal manifestations including primary sclerosing<br />
cholangitis are rarer than in the West. Clinical stratification <strong>of</strong> disease severity guides management. In Japan, leukocytapheresis<br />
is a treatment option. Access to biologic agents remains limited due to high cost and concern over opportunistic infections.<br />
The high endemic rates <strong>of</strong> hepatitis B virus infection require stringent screening before initiating immune-suppressive agents.<br />
Vaccination and prophylactic therapies should be initiated on a case-by-case basis and in accordance with local practice.<br />
Colorectal cancer complicates chronic colitis. A recent increase in UC is reported in the Asia-Pacific region. These consensus<br />
statements aim to improve the recognition <strong>of</strong> UC and assist clinicians in its management with particular relevance to the<br />
region. © <strong>2010</strong> Journal <strong>of</strong> Gastroenterology and Hepatology Foundation and Blackwell Publishing Asia Pty Ltd.<br />
No.906<br />
Author (s) : Opasanon, S., Muangman, P., Namviriyachote, N.<br />
Title : Clinical effectiveness <strong>of</strong> alginate silver dressing in outpatient management <strong>of</strong> partial-thickness burns<br />
Source : <strong>International</strong> Wound Journal, Volume 7, Issue 6, December <strong>2010</strong>, Pages 467-471<br />
Document Type : Article<br />
Keywords : Alginate silver dressing; Askina Calgitrol Ag®; Burn; Silver sulfadiazine<br />
<strong>Abstract</strong> : Askina Calgitrol Ag® (B. Braun Hospicare Ltd, Collooney Co. Sligo, Ireland), alginate silver wound dressing, is an advanced<br />
wound dressing which combines the potent broad-spectrum antimicrobial action <strong>of</strong> silver with enhanced exudate<br />
management properties <strong>of</strong> calcium alginate and polyurethane foam. The purpose <strong>of</strong> this study was to compare the efficacy<br />
<strong>of</strong> Askina Calgitrol Ag® and 1% silver sulfadiazine (1% AgSD) in the outpatient management <strong>of</strong> partial-thickness burn wounds<br />
at Burn Unit, Siriraj Hospital. A prospective descriptive study was conducted between January 2008 and January 2009 in Burn<br />
Unit, Division <strong>of</strong> Trauma Surgery, Siriraj Hospital, <strong>Mahidol</strong> <strong>University</strong>, Thailand. The 65 patients with partial-thickness burn<br />
wounds, less than 24 hours post-burn injury, had a total body surface area (TBSA%) less than 15% were treated at Siriraj<br />
Outpatient Burn Clinic. All patients were divided into Askina Calgitrol Ag® treated group (30 patients) and 1% AgSD treated<br />
group (35 patients). The data were compared by the demographics including age, gender, % TBSA burn, pain score, number <strong>of</strong><br />
wound dressing change, nursing time and time <strong>of</strong> wound healing. Patients included in both groups were comparable with no<br />
significant differences in demographic data <strong>of</strong> age, gender, location <strong>of</strong> burn and type <strong>of</strong> burn injury (P > 0·05 evaluated by<br />
paired Student's t-test) between both group. The present results showed that average pain scores in the Askina Calgitrol Ag®<br />
treated group were significantly lower than the 1% AgSD treated group (2·23 ± 1·87 versus 6·08 ± 2·33, respectively) between<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
both groups (P < 0·02). Patients treated with Askina Calgitrol Ag® had significantly lower number <strong>of</strong> wound dressing change (P<br />
< 0·02) and nursing time (P < 0·02) compared with 1% AgSD treated group. The Askina Calgitrol Ag® group needed less<br />
frequent wound dressing. Healing time was 7 ± 3·51 days after the application <strong>of</strong> Askina Calgitrol Ag®. This was significantly<br />
shorter than that <strong>of</strong> control wounds (14 ± 4·18 days). Application <strong>of</strong> Askina Calgitrol Ag® leads to a good burn wound<br />
outcome. The present study confirms the effectiveness <strong>of</strong> Askina Calgitrol Ag® in the outpatient management <strong>of</strong> partialthickness<br />
burn wounds. © <strong>2010</strong> Blackwell Publishing Ltd and Medicalhelplines.com Inc.<br />
No.907<br />
Author (s) : Opatrná, S., Chiangjong, W., Korabečná, M., Sintiprungrat, K., Sinchaikul, S., Chen, S.-T., Matějovič, M., Thongboonkerd, V.<br />
Title : Plasma proteome pr<strong>of</strong>iling <strong>of</strong> von Hippel-Lindau disease after total and subtotal nephrectomy: A preliminary study<br />
Source : Clinical Biochemistry, Volume 43, Issue 1-2, January <strong>2010</strong>, Pages 142-149<br />
Document Type : Article<br />
Keywords : Chronic kidney disease; Nephrectomy; Plasma; Proteome; Proteomics; Renal cell carcinoma; Renal failure; von Hippel-<br />
Lindau<br />
<strong>Abstract</strong> : Objectives: Common treatment <strong>of</strong> renal cell carcinoma associated with von Hippel-Lindau (VHL) disease is total (bilateral) or<br />
subtotal nephrectomy. Whereas total nephrectomy is associated with absolutely no residual renal function, subtotal<br />
nephrectomy frequently leads to chronic kidney disease (CKD) with some residual renal functions. However, molecular<br />
mechanisms underlying CKD remain unclear and the diagnosis <strong>of</strong> CKD is frequently accomplished at its late stage. Design and<br />
methods: We performed a plasma proteomics study to compare the plasma proteome pr<strong>of</strong>ile <strong>of</strong> VHL patient who underwent<br />
total nephrectomy to the pr<strong>of</strong>iles <strong>of</strong> VHL patient with subtotal nephrectomy and healthy control. Totally 100 μg proteins<br />
from each sample was resolved by two-dimensional electrophoresis (2-DE) in triplicate and visualized with SYPRO Ruby<br />
fluorescence stain. Results: The normal plasma proteome pr<strong>of</strong>ile markedly differed from the pr<strong>of</strong>iles <strong>of</strong> VHL patients.<br />
Comparative analysis between total versus subtotal nephrectomized patients revealed significant differences in levels <strong>of</strong> 20<br />
plasma proteins. Pathway analysis revealed two important networks involving in lipid metabolism, molecular transport,<br />
carbohydrate metabolism, cellular growth and proliferation, and small molecule biochemistry, in which these identified and<br />
other proteins interplayed. Conclusions: Our data identified potential biomarkers for CKD. Further characterization <strong>of</strong> these<br />
identified proteins might also lead to better understanding <strong>of</strong> molecular mechanisms underlying CKD. © 2009 The Canadian<br />
Society <strong>of</strong> Clinical Chemists.<br />
No.908<br />
Author (s) : Overman, A., Bumrungpert, A., Kennedy, A., Martinez, K., Chuang, C.-C., West, T., Dawson, B., Jia, W., McIntosh, M.<br />
Title : Polyphenol-rich grape powder extract (GPE) attenuates inflammation in human macrophages and in human adipocytes<br />
exposed to macrophage-conditioned media<br />
Source : <strong>International</strong> Journal <strong>of</strong> Obesity, Volume 34, Issue 5, May <strong>2010</strong>, Pages 800-808<br />
Document Type : Article<br />
Keywords : Adipocytes; Grape powder extract; Inflammation; Insulin resistance; Macrophages<br />
<strong>Abstract</strong> : Background:Obesity-associated inflammation is characterized by an increased abundance <strong>of</strong> macrophages (Ms) in white<br />
adipose tissue (WAT), leading to the production <strong>of</strong> inflammatory cytokines, chemokines and prostaglandins (PGs) that can<br />
cause insulin resistance. Grape powder extract (GPE) is rich in phenolic phytochemicals that possess anti-oxidant and antiinflammatory<br />
properties.Objective:We examined the ability <strong>of</strong> GPE to prevent lipopolysaccharide (LPS)-mediated inflammation<br />
in human Ms and silence the cross-talk between human Ms and adipocytes.Design:We investigated the effect <strong>of</strong> GPE<br />
pretreatment on LPS-mediated activation <strong>of</strong> mitogen activated protein kinases (MAPKs), nuclear factor kappa B (NF-B) and<br />
activator protein-1 (AP-1), and induction <strong>of</strong> inflammatory genes in human Ms (that is, differentiated U937 cells). In addition,<br />
we determined the effect <strong>of</strong> GPE pretreatment <strong>of</strong> Ms on inflammation and insulin resistance in primary human adipocytes<br />
incubated with LPS-challenged M-conditioned medium (M-CM).Methods and Results:Pretreatment <strong>of</strong> Ms with GPE attenuated<br />
LPS-induction <strong>of</strong> inflammatory cytokines, such as tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and IL-1Β; chemokines,<br />
such as IL-8 and interferon-γ inducible protein-10 (IP-10); and a marker <strong>of</strong> PG production, cyclooxygenase-2 (COX-2). Grape<br />
powder extract also attenuated LPS activation <strong>of</strong> MAPKs, NF-B and AP-1 (c-Jun), as evidenced by decreased (1)<br />
phosphorylation <strong>of</strong> c-Jun NH 2-terminal kinase (JNK) and p38; (2) degradation <strong>of</strong> IBα and activation <strong>of</strong> an NF-B reporter<br />
construct; and (3) phosphorylation <strong>of</strong> c-Jun and Elk-1. Using LPS-challenged M-CM, GPE pretreatment attenuated M-mediated<br />
inflammatory gene expression, activation <strong>of</strong> an NF-B reporter and suppression <strong>of</strong> insulin-stimulated glucose uptake in human<br />
adipocytes.Conclusion:Collectively, these data demonstrate that GPE attenuates LPS-mediated inflammation in Ms, possibly<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
by decreasing the activation <strong>of</strong> MAPKs, NF-B and AP-1, and that GPE decreases the capacity <strong>of</strong> LPS-stimulated Ms to inflame<br />
adipocytes and cause insulin resistance. © <strong>2010</strong> Macmillan Publishers Limited All rights reserved.<br />
No.909<br />
Author (s) : Oyama, Y., Hashiguchi, T., Taniguchi, N., Tancharoen, S., Uchimura, T., Biswas, KK., Kawahara, K., Nitanda, T., Umekita, Y., Lotz,<br />
M., Maruyama, I.<br />
Title : High-mobility group box-1 protein promotes granulomatous nephritis in adenine-induced nephropathy<br />
Source : LABORATORY INVESTIGATION, Volume: 90, Issue: 6, Pages: 853-866, DOI: 10.1038/labinvest.<strong>2010</strong>.64, Published: JUN <strong>2010</strong><br />
Document Type : Article<br />
Keywords : chronic inflammation; granulomatous nephritis; HMGB1; macrophages; MCP-1; renal tubular epithelial cells<br />
<strong>Abstract</strong> : Granulomatous nephritis can be triggered by diverse factors and results in kidney failure. However, despite accumulating data<br />
about granulomatous inflammation, pathogenetic mechanisms in nephritis remain unclear. The DNA-binding high-mobility<br />
group box-1 protein (HMGB1) initiates and propagates inflammation when released by activated macrophages, and functions<br />
as an 'alarm cytokine' signaling tissue damage. In this study, we showed elevated HMGB1 expression in renal granulomas in<br />
rats with crystal-induced granulomatous nephritis caused by feeding an adenine-rich diet. HMGB1 levels were also raised in<br />
urine and serum, as well as in monocyte chemoattractant protein-1 (MCP-1), a mediator <strong>of</strong> granulomatous inflammation.<br />
Injection <strong>of</strong> HMGB1 worsened renal function and upregulated MCP-1 in rats with crystal-induced granulomatous nephritis.<br />
HMGB1 also induced MCP-1 secretion through mitogen-activated protein kinase (MAPK) and phosphoinositide-3-kinase (PI3K)<br />
pathways in rat renal tubular epithelial cells in vitro. Hmgb1(+/-) mice with crystal-induced nephritis displayed reduced MCP-1<br />
expression in the kidneys and in urine and the number <strong>of</strong> macrophages in the kidneys was significantly decreased. We<br />
conclude that HMGB1 is a new mediator involved in crystal-induced nephritis that amplifies granulomatous inflammation in a<br />
cycle where MCP-1 attracts activated macrophages, resulting in excessive and sustained HMGB1 release. HMGB1 could be a<br />
novel target for inhibiting chronic granulomatous diseases. Laboratory Investigation (<strong>2010</strong>) 90, 853-866;<br />
doi:10.1038/labinvest.<strong>2010</strong>.64; published online 15 March <strong>2010</strong><br />
No.910<br />
Author (s) : Oyomopito, R., Lee, M.P., Phanuphak, P., Lim, P.L., Ditangco, R., Zhou, J., Sirisanthana, T., Chen, Y.M.A., Pujari, S., Kumarasamy,<br />
N., Sungkanuparph, S., Lee, C.K.C., Kamarulzaman, A., Oka, S., Zhang, F.J., Mean, C.V., Merati, T., Tau, G., Smith, J., Li, P.C.K.<br />
Title : Measures <strong>of</strong> site resourcing predict virologic suppression, immunologic response and HIV disease progression following highly<br />
active antiretroviral therapy (HAART) in the TREAT Asia HIV Observational Database (TAHOD)<br />
Source : HIV Medicine, Volume 11, Issue 8, September <strong>2010</strong>, Pages 519-529<br />
Document Type : Article<br />
Keywords : Antiretroviral therapy; Asia; CD4 counts; Diagnostic monitoring; Viral load<br />
<strong>Abstract</strong> : Objectives: Surrogate markers <strong>of</strong> HIV disease progression are HIV RNA in plasma viral load (VL) and CD4 cell count (immune<br />
function). Despite improved international access to antiretrovirals, surrogate marker diagnostics are not routinely available in<br />
resource-limited settings. Therefore, the objective was to assess effects <strong>of</strong> economic and diagnostic resourcing on patient<br />
treatment outcomes. Methods: Analyses were based on 2333 patients initiating highly active antiretroviral therapy (HAART)<br />
from 2000 onwards. Sites were categorized by World Bank country income criteria (high/low) and annual frequency <strong>of</strong> VL (≥3,<br />
1-2 or
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.911<br />
Author (s) : Pagès, M., Chaval, Y., Herbreteau, V., Waengsothorn, S., Cosson, J.-F., Hugot, J.-P., Morand, S., Michaux, J.<br />
Title : Revisiting the taxonomy <strong>of</strong> the Rattini tribe: A phylogeny-based delimitation <strong>of</strong> species boundaries<br />
Source : BMC Evolutionary Biology, Volume 10, Issue 1, <strong>2010</strong>, Article number 184<br />
Document Type : Article<br />
Keywords : biodiversity, data set, disease transmission, DNA fingerprinting, economic development, global change, host-pathogen<br />
interaction, morphology, phylogenetics, phylogeny, rodent, taxonomy, type specimen; Southeast Asia; mitochondrial DNA;<br />
animal, article, Bayes theorem, cell nucleus, classification, DNA sequence, genetics, molecular<br />
evolution, phylogeny, rat, sequence alignment, Southeast Asia, species difference, statistical model; Animals, Asia,<br />
Southeastern, Bayes Theorem, Cell Nucleus, DNA, Mitochondrial, Evolution, Molecular, Likelihood<br />
Functions, Phylogeny, Rats, Sequence Alignment, Sequence Analysis, DNA, Species Specificity<br />
<strong>Abstract</strong> : Background. Rodents are recognized as hosts for at least 60 zoonotic diseases and may represent a serious threat for human<br />
health. In the context <strong>of</strong> global environmental changes and increasing mobility <strong>of</strong> humans and animals, contacts between<br />
pathogens and potential animal hosts and vectors are modified, amplifying the risk <strong>of</strong> disease emergence. An accurate<br />
identification <strong>of</strong> each rodent at a specific level is needed in order to understand their implications in the transmission <strong>of</strong><br />
diseases. Among the Muridae, the Rattini tribe encompasses 167 species inhabiting South East Asia, a hotspot <strong>of</strong> both<br />
biodiversity and emerging and re-emerging diseases. The region faces growing economical development that affects habitats,<br />
biodiversity and health. Rat species have been demonstrated as significant hosts <strong>of</strong> pathogens but are still difficult to<br />
recognize at a specific level using morphological criteria. DNA-barcoding methods appear as accurate tools for rat species<br />
identification but their use is hampered by the need <strong>of</strong> reliable identification <strong>of</strong> reference specimens. In this study, we<br />
explore and highlight the limits <strong>of</strong> the current taxonomy <strong>of</strong> the Rattini tribe. Results. We used the DNA sequence information<br />
itself as the primary information source to establish group membership and estimate putative species boundaries. We<br />
sequenced two mitochondrial and one nuclear genes from 122 rat samples to perform phylogenetic reconstructions. The<br />
method <strong>of</strong> Pons and colleagues (2006) that determines, with no prior expectations, the locations <strong>of</strong> ancestral nodes defining<br />
putative species was then applied to our dataset. To give an appropriate name to each cluster recognized as a putative<br />
species, we reviewed information from the literature and obtained sequences from a museum holotype specimen following<br />
the ancient DNA criteria. Conclusions. Using a recently developed methodology, this study succeeds in refining the taxonomy<br />
<strong>of</strong> one <strong>of</strong> the most difficult groups <strong>of</strong> mammals. Most <strong>of</strong> the species expected within the area were retrieved but new<br />
putative species limits were also indicated, in particular within Berylmys and Rattus genera, where future taxonomic studies<br />
should be directed. Our study lays the foundations to better investigate rodent-born diseases in South East Asia and<br />
illustrates the relevance <strong>of</strong> evolutionary studies for health and medical sciences. © <strong>2010</strong> Pagès et al; licensee BioMed Central<br />
Ltd.<br />
No.912<br />
Author (s) : Pagnarith, Y., Kumar, V., Thaipadungpanit, J., Wuthiekanun, V., Amornchai, P., Sin, L., Day, N.P., Peacock, S.J.<br />
Title : Emergence <strong>of</strong> pediatric melioidosis in Siem Reap, Cambodia<br />
Source : American Journal <strong>of</strong> Tropical Medicine and Hygiene, Volume 82, Issue 6, June <strong>2010</strong>, Pages 1106-1112<br />
Document Type : Article<br />
Keywords : amoxicillin plus clavulanic acid, ceftazidime, ceftriaxone, cipr<strong>of</strong>loxacin, cloxacillin; adolescent, antimicrobial<br />
therapy, article, awareness, bacterial infection, bacterial meningitis, bacterial pneumonia, bacterium culture, Burkholderia<br />
infection, Burkholderia pseudomallei, Cambodia, cause <strong>of</strong> death, child, childhood disease, childhood mortality, chronic otitis<br />
media, chronic suppurative otitis media, clinical article, drug substitution, drug withdrawal, female, hospital<br />
admission, human, juvenile rheumatoid arthritis, kidney disease, male, melioidosis, parotitis, preschool child, respiratory<br />
failure, school child, thalassemia, tuberculous spondylitis; Adolescent, Anti-Bacterial Agents, Bacterial Typing<br />
Techniques, Burkholderia pseudomallei, Cambodia, Child, Child,<br />
Preschool, Female, Genotype, Humans, Male, Melioidosis, Phylogeny<br />
<strong>Abstract</strong> : We describe the first cases <strong>of</strong> pediatric melioidosis in Cambodia. Thirty-nine cases were diagnosed at the Angkor Hospital for<br />
Children, Siem Reap, between October 2005 and December 2008 after the introduction <strong>of</strong> microbiology capabilities. Median<br />
age was 7.8 years (range = 1.6-16.2 years), 15 cases were male (38%), and 4 cases had pre-existing conditions that may have<br />
pre-disposed the patient to melioidosis. Infection was localized in 27 cases (69%) and disseminated in 12 cases (31%). Eleven<br />
cases (28%) were treated as outpatients, and 28 (72%) cases were admitted. Eight children (21%) died a median <strong>of</strong> 2 days<br />
after admission; seven deaths were attributable to melioidosis, all <strong>of</strong> which occurred in children receiving suboptimal<br />
336
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
antimicrobial therapy and before bacteriological culture results were available. Our findings indicate the need for heightened<br />
awareness <strong>of</strong> melioidosis in Cambodia, and they have led us to review microbiology procedures and antimicrobial prescribing<br />
<strong>of</strong> suspected and confirmed cases. Copyright © <strong>2010</strong> by The American Society <strong>of</strong> Tropical Medicine and Hygiene.<br />
No.913<br />
Author (s) : Pakakasama, S., Veerakul, G., Sosothikul, D., Chainansamit, S.-O., Laosombat, V., Thanarattanakorn, P., Lumkul, R., Wiangnon, S.,<br />
Wangruangsathit, S., Narkbunnam, N., Kanjanapongkul, S.<br />
Title : Late effects in survivors <strong>of</strong> childhood acute lymphoblastic leukemia: A study from Thai Pediatric Oncology Group<br />
Source : <strong>International</strong> Journal <strong>of</strong> Hematology, Volume 91, Issue 5, June <strong>2010</strong>, Pages 850-854<br />
Document Type : Article<br />
Keywords : Acute lymphoblastic leukemia; ALL; Late effects; Survivors; Thai<br />
<strong>Abstract</strong> : Survivors <strong>of</strong> childhood acute lymphoblastic leukemia (ALL) are at risk <strong>of</strong> long-term late effects. Therefore, systematic<br />
screenings <strong>of</strong> the late complications are essential. The objective <strong>of</strong> this study was to determine the prevalence <strong>of</strong> late effects<br />
<strong>of</strong> Thai children and adolescents after completion <strong>of</strong> ALL therapy. We performed a cross-sectional study for evaluation <strong>of</strong> the<br />
late effects in ALL survivors who came for follow-up at 10 pediatric oncology centers in Thailand. We evaluated the<br />
treatment-related late complications <strong>of</strong> children and adolescents who had finished ALL treatment for at least 2 years.<br />
Demographic data, treatment modalities, and late effects were recorded and analyzed. There were 258 survivors with a<br />
median age <strong>of</strong> 12.2 years (range 3.6-23.3 years). The median follow-up time was 7.2 years (range 2-17.5 years). Forty-seven<br />
percent (122 cases) suffered from at least one late effect. Overweight/obesity was the most common late effect. Radiation <strong>of</strong><br />
central nervous system was a significant risk factor for overweight/obesity (OR 1.97, 95% CI 1.02-3.81) and educational<br />
problems (OR 4.3, 95% CI 1.32-14.02). Our data have demonstrated a significant prevalence <strong>of</strong> late effects after childhood ALL<br />
therapy. A long-term follow-up program for survivors <strong>of</strong> childhood cancer is therefore needed in our country. © <strong>2010</strong> The<br />
Japanese Society <strong>of</strong> Hematology.<br />
No.914<br />
Author (s) : Pakakasama, S.; Surayuthpreecha, K.; Pandee, U.; Anurathpan, U.; Maleevan, V.; Udomsubprayakul, U.; Butthep, P.;<br />
Santanirand, P.; Sirachainan, N.; Hongeng, S.<br />
Title : IMPLEMENTATION OF A CLINICAL PRACTICE GUIDELINE FOR CHILDREN WITH CANCER PRESENTING WITH FEVER AT EMERGENCY<br />
ROOM<br />
Source : HAEMATOLOGICA-THE HEMATOLOGY JOURNAL, Volume: 95, Supplement: 2, Pages: 101-101, Meeting <strong>Abstract</strong>: 0258,<br />
Published: JUN <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.915<br />
Author (s) : Pakdee, W., Waikagul, J., Kalambaheti, T., Ito, A., Dekumyoy, P.<br />
Title : Iso-electricfocusing <strong>of</strong> bithynia snail antigens for IGG-and IGG1-4-elisa detection <strong>of</strong> human opisthorchiasis<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 4, July <strong>2010</strong>, Pages 813-820<br />
Document Type : Article<br />
Keywords : Bithynia antigen; IGG1-elisa; Liquid-phase iso-electricfocusing; Opisthorchiasis<br />
<strong>Abstract</strong> : Diagnosis <strong>of</strong> opisthorchiasis is confirmed by the presence <strong>of</strong> characteristic eggs and worms. However, misdiagnosis may occur<br />
in light infections, and also due to the morphological similarity <strong>of</strong> opisthorchid eggs to other species. A finding <strong>of</strong> specific<br />
immune mediators can help confirm infection. This study used indirect ELISA to detect total IGG and IGG1-4 with selected<br />
antigens <strong>of</strong> Bithynia siamensis goniomphalos extract, which were derived by liquid-phase isoelectricfocusing (IFE). Antigens<br />
(Iso-AgF) from 20 IEF fractionated fractions were selected based on a high ELISA-OD ratio between pooled-positive and<br />
poolednegative sera. Iso-AgF 7, 7, 6, 2, and 10 resulted in high OD-ratio to total IGG, IGG1, 2, 3, and 4, respectively. A full-scale<br />
ELISA was conducted with sera from 50 opisthorchiasis cases, 196 from other parasitic-disease cases, and 35 healthy controls.<br />
Iso-AgF7 to IGG1 showed the best result, with sensitivity, specificity, positive and negative predictive value <strong>of</strong> 100, 96, 86, and<br />
100%, respectively, at a cut-<strong>of</strong>f 0.221. Low cross-reactivity to IGG1 was found in one case each <strong>of</strong> gnathostomiasis,<br />
trichinellosis, toxocariasis, angiostrongyliasis, bancr<strong>of</strong>tian filariasis, enterobiasis, neurocysticercosis, and taeniasis. Thus, Iso-AgF7<br />
to IGG1 was a good candidate antigen to be developed for detection <strong>of</strong> antibodies against Opisthorchis viverrini.<br />
337
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.916<br />
Author (s) : Panchan, W., Chiampanichayakul, S., Snyder, D.L., Yodbuntung, S., Pohmakotr, M., Reutrakul, V., Jaipetch, T., Kuhakarn, C.<br />
Title : Facile oxidative hydrolysis <strong>of</strong> acetals to esters using hypervalent iodine(III)/LiBr combination in water<br />
Source : Tetrahedron, Volume 66, Issue 14, 3 April <strong>2010</strong>, Pages 2732-2735<br />
Document Type : Article<br />
Keywords : (Diacetoxy)iodobenzene; Acetal; DIB; Hydroxyalkyl carboxylic esters; Oxidative hydrolysis<br />
<strong>Abstract</strong> : The combination <strong>of</strong> (diacetoxy)iodobenzene (PhI(OAc)2, DIB) and lithium bromide (LiBr) efficiently oxidized cyclic and acyclic<br />
acetals to the corresponding hydroxyalkyl carboxylic esters and simple esters in good to excellent yields. The merits <strong>of</strong> this<br />
reaction are that it employs commercially available and non-explosive hypervalent iodine(III) reagent, water as the solvent, a<br />
short reaction time, and mild reaction conditions. © <strong>2010</strong> Elsevier Ltd. All rights reserved.<br />
No.917<br />
Author (s) : Pandee, U.; Rungryeangworanon, K.<br />
Title : RISK FACTORS AND PROGNOSTIC PREDICTORS OF UNEXPECTED ADMISSION WITHIN 24 HOURS AFTER EMERGENCY<br />
DEPARTMENT DISCHARGE<br />
Source : CHILD CARE HEALTH AND DEVELOPMENT, Volume: 36, Supplement: 1, Pages: 95-96, Published: JAN <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.918<br />
Author (s) : Pandee, U.; Savatmongkornkul, S.; Arj-Ong, S.<br />
Title : THE RESPONSE OF HEART RATE IN ACUTE FEBRILE CHILDREN AT EMERGENCY ROOM<br />
Source : ACTA PAEDIATRICA, Volume: 99, Supplement: 462, Pages: 54-54, Published: DEC <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.919<br />
Author (s) : Pandian, G.N., Ishikawa, T., Vaijayanthi, T., Hossain, D.M., Yamamoto, S., Nishiumi, T., Angsuthanasombat, C., Haginoya, K.,<br />
Mitsui, T., Hori, H.<br />
Title : Formation <strong>of</strong> macromolecule complex with bacillus thuringiensis Cry1A toxins and chlorophyllide binding 252-kDa lipocalinlike<br />
protein locating on bombyx mori midgut membrane<br />
Source : Journal <strong>of</strong> Membrane Biology, Volume 237, Issue 2-3, October <strong>2010</strong>, Pages 125-136<br />
Document Type : Article<br />
Keywords : Antimicrobial activity; Bacillus thuringiensis Cry1A toxin; Chlorophyllide-binding protein; Insect immunity; Insect midgut<br />
membrane; Red fluorescent protein<br />
<strong>Abstract</strong> : P252, a 252-kDa Bombyx mori protein located on the larval midgut membrane, has been shown to bind strongly with Bacillus<br />
thuringiensis Cry1A toxins (Hossain et al. Appl Environ Microbiol 70:4604-4612, 2004). P252 was also shown to bind<br />
chlorophyllide (Chlide) to form red fluorescence-emitting complex Bm252RFP with significant antimicrobial activity (Pandian et<br />
al. Appl Environ Microbiol 74:1324-1331, 2008). In this article, we show that Cry1A toxin bound with Bm252RFP and<br />
Bm252RFP-Cry1A macrocomplex, with both antimicrobial and insecticidal activities, was formed. The insecticidal activity <strong>of</strong><br />
Bm252RFP-Cry1Ab was reduced from an LD50 <strong>of</strong> 1.62 to 5.05 μg, but Bm252RFP-Cry1Aa and Bm252RFP-Cry1Ac did not show<br />
such reduction. On the other hand, the antimicrobial activity <strong>of</strong> Bm252RFP-Cry1Ab was shown to retain almost the same<br />
activity as Bm252RFP, while the other two complexes lost around 30% activity. The intensity <strong>of</strong> photo absorbance and<br />
fluorescence emission <strong>of</strong> Bm252RFP-Cry1Ab were significantly reduced compared to those <strong>of</strong> the other two complexes.<br />
Circular dichroism showed that the contents <strong>of</strong> Cry1Ab α-helix was significantly decreased in Bm252RFP-Cry1Ab but not in<br />
the other two toxins. These data suggested that the reduction <strong>of</strong> contents <strong>of</strong> α-helix in Cry1Ab affected the insecticidal<br />
activity <strong>of</strong> the macrocomplex but did not alter the antimicrobial moiety in the macrocomplex <strong>of</strong> Bm252RFP-Cry1Ab. © <strong>2010</strong><br />
Springer Science+Business Media, LLC.<br />
338
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.920<br />
Author (s) : Panich, U., Kongtaphan, K., Onkoksoong, T., Jaemsak, K., Phadungrakwittaya, R., Thaworn, A., Akarasereenont, P.,<br />
Wongkajornsilp, A.<br />
Title : Modulation <strong>of</strong> antioxidant defense by Alpinia galanga and Curcuma aromatica extracts correlates with their inhibition <strong>of</strong> UVAinduced<br />
melanogenesis<br />
Source : Cell Biology and Toxicology, Volume 26, Issue 2, April <strong>2010</strong>, Pages 103-116<br />
Document Type : Article<br />
Keywords : Antioxidant defenses; Medicinal plants; Melanogenesis; Ultraviolet A<br />
<strong>Abstract</strong> : Ultraviolet A (UVA) irradiation is suggested to contribute to melanogenesis through promoting cellular oxidative stress and<br />
impairing antioxidant defenses. An overproduction <strong>of</strong> melanin can be associated with melanoma skin cancer and<br />
hyperpigmentation. Therefore, developing effective antimelanogenic agents is <strong>of</strong> importance. Alpinia galanga (AG) and<br />
Curcuma aromatica (CA) are traditional medicinal plants widely used for skin problems. Hence, this study investigated the<br />
antimelanogenic effects <strong>of</strong> AG and CA extracts (3.8-30 μg/ml) by assessing tyrosinase activity, tyrosinase mRNA levels, and<br />
melanin content in human melanoma cells (G361) exposed to UVA. The roles in protecting against melanogenesis were<br />
examined by evaluating their inhibitory effects on UVA-induced cellular oxidative stress and modulation <strong>of</strong> antioxidant<br />
defenses including antioxidant enzymes, catalase (CAT) and glutathione peroxidase (GPx), and intracellular glutathione (GSH).<br />
In addition, possible active compounds accountable for biological activities <strong>of</strong> the extracts were identified by thin layer<br />
chromatography (TLC)-densitometric analysis. Our study demonstrated that UVA (8 J/cm2) induced both tyrosinase activity<br />
and mRNA levels and UVA (16 J/cm2)-mediated melanin production were suppressed by the AG or CA extracts at<br />
noncytotoxic concentrations. Both extracts were able to protect against UVA-induced cellular oxidant formation and<br />
depletion <strong>of</strong> CAT and GPx activities and GSH content in a dose-dependent manner. Moreover, TLC-densitometric analysis<br />
detected the presence <strong>of</strong> eugenol and curcuminoids in AG and CA, respectively. This is the first report representing promising<br />
findings on AG and CA extract-derived antityrosinase properties correlated with their antioxidant potential. Inhibiting cellular<br />
oxidative stress and improving antioxidant defenses might be the mechanisms by which the extracts yield the protective<br />
effects on UVA-dependent melanogenesis. © 2009 Springer Science+Business Media B.V.<br />
No.921<br />
Author (s) : Panich, U.; Tangsupa-a-nan, V.; Onkoksoong, T.; Limsaengurai, S.; Akarasereenont, P.<br />
Title : Gallic acid Inhibits UVA-induced Melanogenesis Possibly Through Up-regulation <strong>of</strong> Glutathione-dependent Antioxidant System<br />
Source : FREE RADICAL BIOLOGY AND MEDICINE, Volume: 49, Supplement: 1, Pages: S193-S193, DOI:<br />
10.1016/j.freeradbiomed.<strong>2010</strong>.10.556, Published: <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.922<br />
Author (s) : Panijpan, B.<br />
Title : Biophysics education<br />
Source : AIP Conference Proceedings, Volume 1263, <strong>2010</strong>, Pages 9-11<br />
Document Type : Conference Paper<br />
Keywords : Biomolecular Structure; Biophysical Chemistry; Education; Molecular Biophysics<br />
<strong>Abstract</strong> : Many topics in basic physics are presented to the students based on examples that they find too intangible, uninteresting and<br />
unrelated to the real world. We propose here some examples from the biological world that can equally be used to<br />
illustrate seemingly arcane physical principles and laws [1] [2]. Energetic aspects <strong>of</strong> the Krebs Cycle, the Calvin Cycle, and the<br />
ion pump can make learning <strong>of</strong> thermodynamics and electricity much more realistic. The contraction relaxation cycle <strong>of</strong> a<br />
muscle sarcomere can be used to represent that <strong>of</strong> a machine "photographs" <strong>of</strong> the sarcomere and large biopolymers such as<br />
ordered protein and nucleic acid structures illustrate Bragg's Law about regular submicron distances as well as those from<br />
crystals <strong>of</strong> inorganic salts. Movements <strong>of</strong> nanostructures <strong>of</strong> the proton pump subunits and sliding filaments are just as good<br />
for calculation <strong>of</strong> the moment <strong>of</strong> inertia and mechanical energy involved. Transport <strong>of</strong> sugars made from photosynthesis in<br />
the leaf into the fruit can pose problems about concentration gradient very realistically. Aspect about light such as absorption<br />
and fluorescence <strong>of</strong> biological molecules such as chlorophylls and rhodopsins should make such phenomena more<br />
interesting and real. Interference <strong>of</strong> visible light from feathers and scales should also be used to showchanges <strong>of</strong> apparent<br />
339
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
colors and patterns when viewed from different angles. Circular dichroism and streaming birefringence <strong>of</strong> biological<br />
macromolecules should make students appreciate polarization better. © <strong>2010</strong> American Institute <strong>of</strong> Physics.<br />
No.923<br />
Author (s) : Panjaburee, P., Hwang, G.-J., Triampo, W., Shih, B.-Y.<br />
Title : A multi-expert approach for developing testing and diagnostic systems based on the concept-effect model<br />
Source : Computers and Education, Volume 55, Issue 2, September <strong>2010</strong>, Pages 527-540<br />
Document Type : Article<br />
Keywords : Computer-assisted learning; Computer-based testing; Concept-effect relationships; Multiple expert systems<br />
<strong>Abstract</strong> : With the popularization <strong>of</strong> computer and communication technologies, researchers have attempted to develop computerassisted<br />
testing and diagnostic systems to help students improve their learning performance on the Internet. In developing a<br />
diagnostic system for detecting students' learning problems, it is difficult for individual teachers to address the exact<br />
relationships between the test items and the concepts. To cope with this problem, this study proposes an innovative<br />
approach to eliciting and integrating the weightings <strong>of</strong> test item-concept relationships from multiple experts. Based on the<br />
proposed approach, a testing and diagnostic system has been implemented; moreover, an experiment was conducted to<br />
evaluate the performance <strong>of</strong> our approach. By analyzing the results from four groups <strong>of</strong> students using learning suggestions<br />
provided by different models, it was found that the learning performance <strong>of</strong> the students who received learning suggestions<br />
by applying the innovative approach was significantly better than for those who received guidance based on the original<br />
model. © <strong>2010</strong> Elsevier Ltd. All rights reserved.<br />
No.924<br />
Author (s) : Pannakapitak, N.; Pacharn, P.; Visitsunthorn, N.; Jirapongsananuruk, O.; Vichyanond, P.<br />
Title : Accuracy Of Skin Prick Test Using In-house Wheat Extract For The Diagnosis Of Ige-mediated Wheat Allergy: A Pilot Study<br />
Source : JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, Volume: 125, Issue: 2, Supplement: 1, Pages: AB84-AB84, Published: FEB<br />
<strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.925<br />
Author (s) : Panunto, W., Jaijoy, K., Lerdvuthisopon, N., Lertprasertsuke, N., Jiruntanat, N., Soonthornchareonnon, N., Sireeratawong, S.<br />
Title : Acute and chronic toxicity studies <strong>of</strong> the water extract from dried fruits <strong>of</strong> Terminalia chebula Rezt. in rats<br />
Source : <strong>International</strong> Journal <strong>of</strong> Applied Research in Natural Products, Volume 3, Issue 4, <strong>2010</strong>, Pages 36-43<br />
Document Type : Article<br />
Keywords : Acute toxicity; Chronic toxicity; Terminalia chebula Retz.<br />
<strong>Abstract</strong> : Terminalia chebula Retz. has been extensively used in traditional medicine for laxative, carminative, astringent, expectorant,<br />
and tonic. The chronic toxicity <strong>of</strong> water extract has not yet been studied. To evaluate its safety, the water extract from the<br />
dried fruits <strong>of</strong> T. chebula was orally administrated in rats. For acute toxicity, a single oral administration was performed at a<br />
dose <strong>of</strong> 5,000 mg/kg body weight (five females, five males). The study <strong>of</strong> chronic toxicity was determined by oral feeding both<br />
female and male rats (ten females, ten males) daily with the test substance at the dose <strong>of</strong> 300, 600 and 1,200 mg/kg body<br />
weight continuously for 270 days. The results <strong>of</strong> acute toxicity showed no signs <strong>of</strong> toxicity such as general behavior changes,<br />
mortality, changes on gross appearance or histophatological changes <strong>of</strong> the internal organs <strong>of</strong> rats. The examinations <strong>of</strong> signs<br />
<strong>of</strong> chronic toxicity showed no abnormalities in the test groups as compared to the controls. Hematological and blood<br />
chemical values in treated groups were normal in comparison with the control group. Non-toxicity effect <strong>of</strong> T. chebula was<br />
present as no changes in body weight, internal organ weight, and general behaviors. Macroscopic or microscopic <strong>of</strong> internal<br />
organs or tissues in treated rats showed no changes. Therefore, the water extract <strong>of</strong> T. chebula given orally to female and<br />
male rats did not produce both acute and chronic toxicities in rats. Industrial relevance: The use <strong>of</strong> medicinal plants and<br />
preparations derived from them as dietary supplements, nutraceuticals, functional foods and herbal medicinal products has<br />
become more widely accepted in developing countries. Therefore, it is important to evaluate the adverse effects <strong>of</strong> these<br />
plants and their preparations. Among them, T. chebula has received high attention because decoction <strong>of</strong> its fresh and dried<br />
fruits is used in traditional medicine for the treatment <strong>of</strong> several illnesses such as laxative, carminative, expectorant,<br />
astringent and tonic. The data <strong>of</strong> acute and chronic toxicity on T. chebula should be obtained in order to increase the<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
confidence in its safety to human, particularly for use in the development <strong>of</strong> pharmaceutical products. © <strong>2010</strong>-2011. IJARNP-<br />
HS <strong>Publications</strong>.<br />
No.926<br />
Author (s) : Panya, A., Sawasdee, N., Srisawat, C., Yenchitsomanus, P.-T., Peerapittayamongkol, C.<br />
Title : Expression <strong>of</strong> zinc finger and homeobox 2 in erythroleukaemic cells and gamma-globin expression<br />
Source : ScienceAsia, Volume 36, Issue 4, December <strong>2010</strong>, Pages 342-345<br />
Document Type : Article<br />
Keywords : γ; -globin; Foetal haemoglobin; K562; QTL; ZHX2<br />
<strong>Abstract</strong> : During the last few decades, foetal haemoglobin reactivation has been considered as a promising intervention to treat βthalassaemia<br />
and sickle-cell anaemia. Variable foetal haemoglobin (Hb F, α 2γ2) production among individuals is under<br />
control <strong>of</strong> distinct chromosome regions, namely, the quantitative trait loci (QTL). One <strong>of</strong> the QTL affecting Hb F levels on<br />
chromosome 8q has never been explored. The zinc fingers and homeodomains 2 (ZHX2) transcription factor located at 8q<br />
position is remarkably down-regulated in hereditary persistence <strong>of</strong> foetal haemoglobin, indicating an inverse correlation<br />
between the γ-globin and ZHX2 expression. Here we studied the effect <strong>of</strong> ZHX2 over the γ-globin expression in K562<br />
erythroleukaemic cell line by transient transfection with a ZHX2 expression plasmid. At 24 h after transfection, the relative<br />
expression <strong>of</strong> endogenous γ-globin mRNA had been reduced to 0.401 ± 0.08 as assessed by real-time PCR. Our finding<br />
suggests that the repressive effect on γ-globin probably results from the presence <strong>of</strong> ZHX2 and supports its possible<br />
involvement in the regulation <strong>of</strong> γ-globin expression.<br />
No.927<br />
Author (s) : Panyarachun, B., Sobhon, P., Tinikul, Y., Chotwiwatthanakun, C., Anupunpisit, V., Anuracpreeda, P.<br />
Title : Paramphistomum cervi: Surface topography <strong>of</strong> the tegument <strong>of</strong> adult fluke<br />
Source : Experimental Parasitology, Volume 125, Issue 2, June <strong>2010</strong>, Pages 95-99<br />
Document Type : Article<br />
Keywords : Paramphistomum cervi; Scanning electron microscopy; Tegument<br />
<strong>Abstract</strong> : Adult Paramphistomum cervi or rumen fluke are pear-shaped, slightly concave ventrally and convex dorsally. The worm<br />
measures about 5-13. mm in length and 2-5. mm in width across the mid-section. As observed by scanning electron<br />
microscopy (SEM), the tegumental surface in all part <strong>of</strong> the body, appears highly corrugated with transverse folds alternating<br />
with grooves and is spineless. At high magnification, the surface <strong>of</strong> the fold is composed <strong>of</strong> micr<strong>of</strong>olds or ridges separated by<br />
microgrooves or pits. Corrugations and invaginations <strong>of</strong> the ventral surface are also more extensive than on the dorsal surface<br />
<strong>of</strong> the body. Both anterior and posterior suckers have thick rims covered with transverse folds without spine. The genital pore<br />
is situated at the anterior third <strong>of</strong> the body. There are two types <strong>of</strong> sensory papillae on the surface: type 1 is bulbous in<br />
shape, measuring 10-15. μm in diameter at the base with nipple-like tips, and type 2 has a similar shape and size and also a<br />
short cilia on top. These sensory papillae usually occur in large clusters, each having between 5 and 20 units depending on<br />
the region <strong>of</strong> the body. Clusters <strong>of</strong> papillae on the ventral surface and around the anterior suckers tend to be more<br />
numerous and larger in size. The dorsal surface <strong>of</strong> the body has the least number <strong>of</strong> papillae. © 2009 Elsevier Inc.<br />
No.928<br />
Author (s) : Paobthong, N., Boonsinsukh, R., Saengsirisuwan, V., Sirisup, S.<br />
Title : Application <strong>of</strong> Wavelet Transform to identify motor unit recruitment pattern<br />
Source : Proceedings <strong>of</strong> <strong>2010</strong> IEEE EMBS Conference on Biomedical Engineering and Sciences, IECBES <strong>2010</strong>, <strong>2010</strong>, Article number<br />
5742236, Pages 242-245<br />
Document Type : Conference Paper<br />
Keywords : aging; motor unit recruitment pattern; wavelet<br />
<strong>Abstract</strong> : Motor unit recruitment pattern represents the time-frequency characteristics <strong>of</strong> muscle contraction. The Continuous Wavelet<br />
Transform (CWT) is a time-frequency signal analysis but its ability to identify motor unit recruitment pattern is still unclear.<br />
The purpose <strong>of</strong> this study was to validate the CWT in identifying the differences in motor unit recruitment pattern, regarding<br />
time-space domains, between young and elderly healthy subjects during maximum voluntary knee extension. Ten elderly<br />
and 10 young subjects participated in this study. They were asked to perform isometric knee extension at 100% maximum<br />
voluntary contraction for 10 seconds. CWT analysis was presented as scalogram. Results showed that the presence <strong>of</strong> red<br />
color spectrum in the young subjects was less than that in the elderly group, indicating that the major recruitments occur<br />
341
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
more frequently in the elderly. When considering the neuronal firing frequency, the elderly subject operates the firing<br />
frequency at a lower range than the young group. The findings in this study confirmed that the CWT can be used to identify<br />
the age-related changes in motor unit recruitment pattern. © <strong>2010</strong> IEEE.<br />
No.929<br />
Author (s) : Parengam, M., Judprasong, K., Srianujata, S., Jittinandana, S., Laoharojanaphand, S., Busamongko, A.<br />
Title : Study <strong>of</strong> nutrients and toxic minerals in rice and legumes by instrumental neutron activation analysis and graphite furnace<br />
atomic absorption spectrophotometry<br />
Source : Journal <strong>of</strong> Food Composition and Analysis, Volume 23, Issue 4, June <strong>2010</strong>, Pages 340-345<br />
Document Type : Article<br />
Keywords : Food analysis; Food composition; Graphite furnace atomic absorption spectrophotometry; Instrumental neutron activation<br />
analysis; Legumes; Nutrient minerals; Rice; Toxic minerals<br />
<strong>Abstract</strong> : Instrumental neutron activation analysis (INAA) is one <strong>of</strong> the high metrological techniques for mineral determination.<br />
Nevertheless, it has some limitations for minerals with low detection limits, such as lead and cadmium, for which graphite<br />
furnace atomic absorption spectrophotometry (GF-AAS) provides reliable outcomes. This study was conducted to determine<br />
nutrients and toxic elements in four varieties <strong>of</strong> rice and six varieties <strong>of</strong> legumes. Rice and legume samples were collected<br />
from Talad-Thai and Yoawarat markets, which were representatives <strong>of</strong> rural and Bangkok areas, respectively. INAA was used<br />
for determination <strong>of</strong> various minerals whereas GF-AAS was used to determine Cd and Pb. In-house reference materials (RMs:<br />
white jasmine rice and soybeans) were prepared and their mineral contents were assigned, along with certified reference<br />
materials, by INAA and GF-AAS with standard addition technique. The INAA and GF-AAS methods were validated by various<br />
certified reference materials which showed a fairly good accuracy, 0.7-4.5% bias for INAA; 1.54 and 6.06% bias for AAS. The<br />
precision <strong>of</strong> INAA and GF-AAS, relative standard deviation (RSD) <strong>of</strong> all studied minerals was good (RSD less than 8.9%). All<br />
varieties <strong>of</strong> rice and legume seeds contain high amounts <strong>of</strong> Ca, K and Mg, whereas trace amounts <strong>of</strong> Al, Br, Cl, Fe, Mn, Zn. Cr,<br />
Cu, I, Mo, Se and Sn were found. Trace amounts <strong>of</strong> As (0.029-0.181. mg/kg) and Cd (0.010-0.025. mg/kg) were detected in all<br />
varieties <strong>of</strong> rice, but not detected in legume seeds, except that slightly higher level <strong>of</strong> Cd were found in soybeans and peanut<br />
(0.022 and 0.085. mg/kg, respectively). Low levels <strong>of</strong> Hg, ranging from 0.196 to 0.352. mg/kg, were detected in all studied<br />
sample, whereas level <strong>of</strong> Pb less than LOD (1. mcg/100. g) was found. To improve the quality <strong>of</strong> analytical mineral data,<br />
these in-house reference materials can be used as a reference material for internal and external quality control systems. ©<br />
<strong>2010</strong> Elsevier Inc.<br />
No.930<br />
Author (s) : Parichatikanond, W., Suthisisang, C., Dhepakson, P., Herunsalee, A.<br />
Title : Study <strong>of</strong> anti-inflammatory activities <strong>of</strong> the pure compounds from Andrographis paniculata (burm.f.) Nees and their effects on<br />
gene expression<br />
Source : <strong>International</strong> Immunopharmacology, Volume 10, Issue 11, November <strong>2010</strong>, Pages 1361-1373<br />
Document Type : Article<br />
Keywords : Andrographis paniculata; Andrographolide; Dehydroandrographolide; Gene expression; Inflammation; Neoandrographolide<br />
<strong>Abstract</strong> : In inflammation, the responses to noxious stimuli are controlled by the highly modulated interactions between various<br />
immune cells and chemical mediators. The purpose <strong>of</strong> this study is to evaluate and compare the anti-inflammatory effect <strong>of</strong><br />
diterpenoids isolated from Andrographis paniculata, including dehydroandrographolide (AP1), andrographolide (AP2) and<br />
neoandrographolide (AP3), on the production <strong>of</strong> inflammatory cytokines and COX activities. Furthermore, the alteration <strong>of</strong><br />
gene expression involved in this activity was investigated in the most potent compound to elucidate the other possible<br />
molecular mechanisms. AP1 (30.1μM; 10μg/ml) and AP2 (28.5μM; 10μg/ml) markedly inhibited COX-1 in ionophore A23187induced<br />
human platelets. AP2 (28.5μM) and AP3 (20.8μM; 10μg/ml) strongly suppressed the LPS-stimulated COX-2 activity in<br />
human blood. In addition, AP2 modulated the level <strong>of</strong> LPS-induced TNF-α, IL-6, IL-1β and IL-10 secretion in human blood in<br />
a concentration-dependent manner. The results revealed that AP2 exhibited the highest efficacy. Therefore, changes in the<br />
levels <strong>of</strong> mRNA transcripts by AP2 were further measured using human cDNA microarrays. The molecular response to AP2 was<br />
complex and mediated by various processes. Among the altered gene expressions, the genes involved in immune and<br />
inflammation processes were selectively down-regulated, such as cytokines and cytokine receptors (TNFSF14, TNF, TNFRSF6,<br />
and IL1A), chemokines (CCL8 and CXCL11), JAK/STAT signaling (JAK3 and STAT5A), TLRs family (TLR4 and TLR8) and NF-κB<br />
(NFKB1). Expression <strong>of</strong> some genes was validated using RT-PCR. The results demonstrated that AP1, AP2 and AP3 exhibited<br />
342
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
the anti-inflammatory effect by interfering COX and inflammatory cytokines and the underlying mechanisms <strong>of</strong> AP2 may be<br />
related to down-expression <strong>of</strong> genes involved in inflammatory cascade. © <strong>2010</strong> Elsevier B.V.<br />
No.931<br />
Author (s) : Pariwatcharakul, P., Chantarat, W., Singhakant, S., Jantarak, L., Suriyachaiwattana, T., Sakulisariyaporn, C., Sitdhiraksa, N.,<br />
Chantakarn, S., Ketumarn, P., Yantadilok, S.<br />
Title : Antipsychotic prescribing pattern for geriatric patients with delirium in Thailand<br />
Source : Clinical Psychopharmacology and Neuroscience, Volume 8, Issue 3, December <strong>2010</strong>, Pages 144-148<br />
Document Type : Conference Paper<br />
Keywords : Antipsychotics; Delirium; Elderly; Geriatric; Prescription pattern<br />
<strong>Abstract</strong> : Objective: To describe the antipsychotic prescribing pattern for the treatment <strong>of</strong> delirium among hospitalized elderly patients<br />
when compared to those younger adults referred to a psychiatric consultation-liaison service in Thailand Methods: All<br />
psychiatric consultations performed upon hospitalized patients at a university hospital in Bangkok, Thailand, during a one-year<br />
period were reviewed. We collected the data from consultation request forms and medical records. Results: Among all 840<br />
patients referred for psychiatric consultation, 656 <strong>of</strong> them had complete medical records available. The consultant<br />
psychiatrists diagnosed 172 (26.2%) <strong>of</strong> them with delirium. After excluding 16 patients with substance-withdrawal delirium,<br />
data on antipsychotics prescription <strong>of</strong> 156 (23.8%) patients were analyzed. The mean age was 61.3+17.6 years old (22-95); 91<br />
(61.9%) were 60 years old or more, and 103 (70.1%) were male. Antipsychotics were prescribed for 85.3% <strong>of</strong> all patients.<br />
Atypical antipsychotics were more commonly prescribed for the elderly (60 years or older) than for the young (51.6% vs<br />
35.4%, p=0.038). Risperidone was the most frequently prescribed antipsychotic for elderly patients accounting for 44% <strong>of</strong><br />
prescriptions. We found negative correlation between age <strong>of</strong> the patients and the dose <strong>of</strong> haloperidol; r=0.233, p=0.043.<br />
Conclusion: To our knowledge, this is the first study on current antipsychotics prescribing practice for the hospitalized elderly<br />
with delirium in a consultation-liaison service in Asia. Although the current evidence show no superiority for atypical<br />
antipsychotics over haloperidol, as well as limited evidence <strong>of</strong> their efficacy and safety in managing delirium, most doctors<br />
had the tendency to prescribe atypical antipsychotics for the hospitalized elderly patients with delirium. Further study is<br />
required to determine the optimal pharmacological therapies for delirium in the elderly. Copyright ©<strong>2010</strong>, Korean College <strong>of</strong><br />
Neuropsychopharmacology.<br />
No.932<br />
Author (s) : Pariwatcharakul, P.; Ratta-apha, W.; Sitdhiraksa, N.; Pornnoppadol, C.; Ketumarn, P.<br />
Title : Benzodiazepine and antidepressant use in geriatric outpatients with generalized anxiety disorder in Thailand<br />
Source : INTERNATIONAL JOURNAL OF NEUROPSYCHOPHARMACOLOGY, Volume: 13, Supplement: 1, Pages: 121-121, Published: <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.933<br />
Author (s) : Park, D.M., Sathornsumetee, S., Rich, J.N.<br />
Title : Medical oncology: Treatment and management <strong>of</strong> malignant gliomas<br />
Source : Nature Reviews Clinical Oncology, Volume 7, Issue 2, February <strong>2010</strong>, Pages 75-77<br />
Document Type : Short Survey<br />
Keywords : angiogenesis inhibitor, bevacizumab, cediranib, irinotecan, isocitrate dehydrogenase, lomustine, phosphatidylinositol 3<br />
kinase, phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase, temozolomide, tirapazamine; cancer genetics, cancer<br />
growth, cancer radiotherapy, cancer stem cell, cancer surgery, clinical trial, gene<br />
mutation, glioblastoma, human, monotherapy, multimodality cancer therapy, nonhuman, priority journal, recurrent<br />
cancer, short survey, treatment failure, treatment response; Angiogenesis Inhibitors, Central Nervous System<br />
Neoplasms, Chemotherapy, Adjuvant, Dacarbazine, Glioblastoma, Humans, Salvage Therapy, Treatment Outcome<br />
<strong>Abstract</strong> : –<br />
No.934<br />
Author (s) : Parks, J., Gyeltshen, T., Prachyawarakorn, V., <strong>Mahidol</strong>, C., Ruchirawat, S., Kittakoop, P.<br />
Title : Glutarimide alkaloids and a terpenoid benzoquinone from Cordia globifera<br />
343
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Source : Journal <strong>of</strong> Natural Products, Volume 73, Issue 5, 28 May <strong>2010</strong>, Pages 992-994<br />
Document Type : Article<br />
Keywords : 4 o methylphorbol 13 acetate 12 myristate, alkaloid derivative, benzoquinone, cordiarimide, cordiarimide B, cyclopropane<br />
derivative, globiferane, glutarimide derivative, meroterpene, scavenger, superoxide, terpenoid derivative, unclassified drug;<br />
article, cell strain HL 60, Cordia, Cordia globifera, cytotoxicity, human, human cell, leukemia cell, nonhuman, plant<br />
root, promyelocytic leukemia; Alkaloids, Benzoquinones, Cordia, Free Radical Scavengers, HL-60 Cells, Humans, Molecular<br />
Structure, Piperidones, Plants, Medicinal, Superoxides, Terpenes, Tetradecanoylphorbol Acetate, Thailand, Xanthine Oxidase;<br />
Cordia<br />
<strong>Abstract</strong> : Three new compounds, a meroterpene (2) having a cyclopropane moiety named globiferane and glutarimide alkaloids named<br />
cordiarimides A (3) and B (4), were isolated from the roots <strong>of</strong> Cordia globifera. Compounds 2-4 exhibited weak cytotoxic<br />
activity. Cordiarimide B (4) exhibited radical scavenging activity, as it inhibited superoxide anion radical formation in the<br />
xanthine/xanthine oxidase (XXO) assay, and also suppressed superoxide anion generation in differentiated HL-60 human<br />
promyelocytic leukemia cells when induced by 12-O-tetradecanoylphorbol-13-acetate (TPA). This is the first report on the<br />
presence <strong>of</strong> glutarimide alkaloids in the genus Cordia. © <strong>2010</strong> The American Chemical Society and American Society <strong>of</strong><br />
Pharmacognosy.<br />
No.935<br />
Author (s) : Pasomsub, E., Sukasem, C., Sungkanuparph, S., Kijsirikul, B., Chantratita, W.<br />
Title : The application <strong>of</strong> artificial neural networks for phenotypic drug resistance prediction: Evaluation and comparison with other<br />
interpretation systems<br />
Source : Japanese Journal <strong>of</strong> Infectious Diseases, Volume 63, Issue 2, <strong>2010</strong>, Pages 87-94<br />
Document Type : Article<br />
Keywords : abacavir, amino acid, amprenavir, antiretrovirus agent, delavirdine, didanosine, efavirenz, indinavir, lamivudine,<br />
lopinavir, nelfinavir, nevirapine, ritonavir, saquinavir, stavudine, zidovudine; antiviral resistance, article, artificial neural<br />
network, controlled study, correlation coefficient, genotype, Human immunodeficiency virus 1, information<br />
system, intermethod comparison, mutational analysis, nonhuman, phenotype, prediction, scoring system, sequence<br />
analysis, support vector machine, validation study; Anti-HIV Agents, Drug Resistance, Viral, Genotype, HIV Infections, HIV-<br />
1, Humans, Microbial Sensitivity Tests, Neural Networks (Computer)<br />
<strong>Abstract</strong> : Although phenotypic resistance testing provides more direct measurement <strong>of</strong> antiretroviral drug resistance than genotypic<br />
testing, it is costly and time-consuming. However, genotypic resistance testing has the advantages <strong>of</strong> being simpler and more<br />
accessible, and it might be possible to use the data obtained for predicting quantitative drug susceptibility to interpret<br />
complex mutation combinations. This study applied the Artificial Neural Network (ANN) system to predict the HIV-1 resistance<br />
phenotype from the genotype. A total <strong>of</strong> 7,598 pairs <strong>of</strong> HIV-1 sequences, with their corresponding phenotypic fold change<br />
values for 14 antiretroviral drugs, were trained, validated, and tested in ANN modeling. The results were compared with the<br />
HIV-SEQ and Geno2pheno interpretation systems. The prediction performance <strong>of</strong> the ANN models was measured by 10-fold<br />
cross-validation. The results indicated that by using the ANN, with an associated set <strong>of</strong> amino acid positions known to<br />
influence drug resistance for individual antiretroviral drugs, drug resistance was accurately predicted and generalized for<br />
individual HIV-1 subtypes. Therefore, high correlation with the experimental phenotype may help physicians choose optimal<br />
therapeutic regimens that might be an option, or supporting system, <strong>of</strong> FDA-approved genotypic resistance testing in heavily<br />
treatment-experienced patients.<br />
No.936<br />
Author (s) : Pathomwichaiwat, T.; Suvitayavat, W.; Sailasuta, A.; Piyachaturawat, P.; Soonthornchareonnon, N.; Prathanturarug, S.<br />
Title : Antiosteoporotic effect <strong>of</strong> Cissus quadrangularis L. extracts in ovariectomized mice<br />
Source : PLANTA MEDICA, Volume: 76, Issue: 12, Pages: 1382-1382, Published: AUG <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.937<br />
Author (s) : Patikarnmonthon, N., Nawapan, S., Buranajitpakorn, S., Charoenlap, N., Mongkolsuk, S., Vattanaviboon, P.<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Copper ions potentiate organic hydroperoxide and hydrogen peroxide toxicity through different mechanisms in Xanthomonas<br />
campestris pv. Campestris<br />
Source : FEMS Microbiology Letters, Volume 313, Issue 1, December <strong>2010</strong>, Pages 75-80<br />
Document Type : Letter<br />
Keywords : AhpC; Copper; Peroxide; Xanthomonas<br />
<strong>Abstract</strong> : Copper (Cu)-based biocides are important chemical controls for both fungal and bacterial diseases in crop fields. Here, we<br />
showed that Cu ions at a concentration <strong>of</strong> 100 μM enhanced t-butyl hydroperoxide (tBOOH) and hydrogen peroxide (H2O2)<br />
killing <strong>of</strong> Xanthomonas campestris pv. campestris through different mechanisms. The addition <strong>of</strong> an antilipid peroxidation<br />
agent (α-tocopherol) and hydroxyl radical scavengers (glycerol and dimethyl sulphoxide) partially protected the bacteria from<br />
the Cu-enhanced tBOOH and H2O2 killing, respectively. Inactivation <strong>of</strong> the alkyl hydroperoxide reductase gene rendered the<br />
mutant vulnerable to lethal doses <strong>of</strong> copper sulphate, which could be alleviated by the addition <strong>of</strong> an H2O2 scavenger<br />
(pyruvate) and α-tocopherol. Taken together, the data suggest that Cu ions influence the killing effect <strong>of</strong> tBOOH through the<br />
stimulation <strong>of</strong> lipid peroxidation, while hydroxyl radical production is the underlying mechanism responsible for the Cu-ionenhanced<br />
H2O2 killing effects. © <strong>2010</strong> Federation <strong>of</strong> European Microbiological Societies.<br />
No.938<br />
Author (s) : Pattanakitsakul, S.-N., Poungsawai, J., Kanlaya, R., Sinchaikul, S., Chen, S.-T., Thongboonkerd, V.<br />
Title : Association <strong>of</strong> Alix with late endosomal lysobisphosphatidic acid is important for dengue virus infection in human endothelial<br />
cells<br />
Source : Journal <strong>of</strong> Proteome Research, Volume 9, Issue 9, 3 September <strong>2010</strong>, Pages 4640-4648<br />
Document Type : Article<br />
Keywords : Alix; dengue; endothelial cells; host responses; LBPA; proteome; proteomics<br />
<strong>Abstract</strong> : The most severe form <strong>of</strong> dengue virus (DENV) infection is dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS),<br />
which is accompanied by increased vascular permeability indicating that endothelial cells are the targets <strong>of</strong> DENV infection.<br />
However, molecular mechanisms underlying DENV replication in endothelial cells remained poorly understood. We therefore<br />
examined changes in subcellular proteomes <strong>of</strong> different cellular compartments (including cytosolic, membrane/organelle,<br />
nucleus, and cytoskeleton) <strong>of</strong> human endothelial (EA.hy926) cells upon DENV2 infection using a 2-DE-based proteomics<br />
approach followed by Q-TOF MS and MS/MS. A total <strong>of</strong> 35 altered proteins were identified in these subcellular locales,<br />
including an increase in the level <strong>of</strong> Alix (apoptosis-linked gene-2-interacting protein X) in the cytosolic fraction <strong>of</strong> DENV2infected<br />
cells compared to mock control cells. Double immun<strong>of</strong>luorescence staining revealed colocalization <strong>of</strong> Alix with late<br />
endosomal lysobisphosphatidic acid (LBPA). This complex has been proposed to be involved in the export <strong>of</strong> DENV proteins<br />
from late endosomes to the cytoplasm. Subsequent functional study revealed that pretreatment with an anti-LBPA antibody<br />
prior to DENV challenge significantly reduced the level <strong>of</strong> viral envelope protein synthesis and DENV replication. Our data<br />
indicate that Alix plays a pivotal role in the early phase <strong>of</strong> DENV replication, particularly when it arrives at the late endosome<br />
stage. Blocking this step may lead to a novel therapeutic approach to reducing the level <strong>of</strong> DENV replication in vivo. © <strong>2010</strong><br />
American Chemical Society.<br />
No.939<br />
Author (s) : Pattanapanyasat, K., Noulsri, E., Lerdwana, S., Sukapirom, K., Onlamoon, N., Tassaneetrithep, B.<br />
Title : The use <strong>of</strong> glutaraldehyde-fixed chicken red blood cells as counting beads for performing affordable single-platform CD4+ tlymphocyte<br />
count in HIV-1-infected patients<br />
Source : Journal <strong>of</strong> Acquired Immune Deficiency Syndromes, Volume 53, Issue 1, January <strong>2010</strong>, Pages 47-54<br />
Document Type : Article<br />
Keywords : AIDS; CD4 T-lymphocyte count; Chicken red blood cell; Flow cytometry; HIV; Microbeads; Single platform<br />
<strong>Abstract</strong> : CD4 T-lymphocyte count is an important marker in management <strong>of</strong> HIV-1-infected patients. The standard single-platform (SP)<br />
flow cytometric (FCM) CD4 testing that uses the known reference microbeads is expensive; more affordable alternatives are<br />
therefore needed. We evaluated the use <strong>of</strong> glutaraldehyde-fixed chicken red blood cells (CRBCs) as counting beads as an<br />
alternative for enumerating CD4 T-lymphocyte counts in 87 HIV-1-infected patients. Linear regression analyses revealed an<br />
excellent correlation <strong>of</strong> the SP FCM using CRBCs with the standard SP bead-based FCM method (percentages, r > 0.99;<br />
absolute counts, r > 0.98) over the entire range including the clinically relevant range. Mean percent bias for the CRBC<br />
method was +0.35% [limits <strong>of</strong> agreement (LOA):-1.86% to +2.57%]. For absolute CD4 T-lymphocytes, the mean biases was-<br />
47.76 cells per microliter (LOA:-191.34 to +98.81 cells/μL) with much lower bias for CD4 T-lymphocyte counts
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
microliter (LOA:-31.92 to +22.95 cells/μL). The use <strong>of</strong> CRBCs is comparable with the use <strong>of</strong> commercial microbeads. This has<br />
resulted in major cost savings to resource-limited countries where the health care system is under increasing pressure to<br />
operate cost effectively. This can greatly facilitate and ensure the success <strong>of</strong> the ongoing antiretroviral therapy program in<br />
these countries. Copyright © 2009 by Lippincott Williams & Wilkins.<br />
No.940<br />
Author (s) : Pattanapanyasat, K., Sratongno, P., Chimma, P., Chitjamnongchai, S., Polsrila, K., Chotivanich, K.<br />
Title : Febrile temperature but not proinflammatory cytokines promotes phosphatidylserine expression on plasmodium falciparum<br />
malaria-infected red blood cells during parasite maturation<br />
Source : Cytometry Part A, Volume 77, Issue 6, June <strong>2010</strong>, Pages 515-523<br />
Document Type : Article<br />
Keywords : Annexin V; Flow cytometry; Hydroethidine; Malaria; Phosphatidylserine; Phospholipids; Plasmodium<br />
falciparum; Proinflammatory cytokine<br />
<strong>Abstract</strong> : Intraerythrocytic maturation <strong>of</strong> the malaria parasite Plasmodium falciparum is associated with pr<strong>of</strong>ound changes in the<br />
asymmetry <strong>of</strong> phospholipids in the lipid bilayer <strong>of</strong> the parasitized red blood cells (pRBCs). These changes may contribute to<br />
adherence <strong>of</strong> pRBCs to endothelial cells. This study investigates the effect <strong>of</strong> febrile temperature and proinflammatory<br />
cytokines on phosphatidylserine (PS) expression on the ex<strong>of</strong>acial surface <strong>of</strong> pRBCs during parasite maturation. The expression<br />
<strong>of</strong> PS on the pRBCs was determined by flow cytometry using fluorescein-labeled annexin V, which specifically binds to PS and<br />
a vital nucleic acid fluorochrome for parasite staining. The results showed that PS expression on the surface <strong>of</strong> pRBCs<br />
increased in association with parasite maturation, especially at the late parasite stage. Furthermore, the growth <strong>of</strong> P.<br />
falciparum also accelerated senescence <strong>of</strong> the uninfected RBCs in parasite cultures. Exposure to febrile temperature led to<br />
significant increases in the expression <strong>of</strong> PS on the surface <strong>of</strong> pRBCs, particularly at the late parasite stage associated with the<br />
virulence strain <strong>of</strong> the parasite. In contrast, proinflammatory cytokines had no detectable effect on PS expression on pRBCs.<br />
These data suggest that PS molecule expression is more dependent on fever, parasitemia, parasite strain, and virulence than<br />
on cytokine exposure. These findings contribute to our understanding <strong>of</strong> the factors that are involved in malaria pathogenesis.<br />
© <strong>2010</strong> <strong>International</strong> Society for Advancement <strong>of</strong> Cytometry.<br />
No.941<br />
Author (s) : Pattanapanyasat, K.; Sratongno, P.; Chimma, P.; Chitjamnongchai, S.; Polsrila, K.; Chotivanich, K.<br />
Title : EFFECT OF TEMPERATURE AND PROINFLAMMATORY CYTOKINES ON PHOSPHATIDYLSERINE EXPRESSION ON PLASMODIUM<br />
FALCIPARUM MALARIA-INFECTED RED BLOOD CELLS DURING PARASITE MATURATION<br />
Source : AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, Volume: 83, Issue: 5, Supplement: S, Pages: 162-162, Meeting<br />
<strong>Abstract</strong>: 540, Published: NOV <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.942<br />
Author (s) : Pattanaprateep, O., Pongcharoensuk, P., Suvanakoot, P., Kaojarern, S.<br />
Title : Pattern <strong>of</strong> statins' utilization at Ramathobodi Hospital, 2005 to 2007<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 10, October <strong>2010</strong>, Pages 1223-1231<br />
Document Type : Article<br />
Keywords : Drug expenditure; Price; Quantity; Statins<br />
<strong>Abstract</strong> : Background: The use <strong>of</strong> statins at Ramathibodi, a teaching hospital, has been rising rapidly. Objective: Determine how price,<br />
volume, and drug mix (use <strong>of</strong> five brands and one generic) affect expenditure. Material and Method: Dispensing records <strong>of</strong> the<br />
six statins were retrieved from database and analyzed for factors contributing to increased drug expense. Results: Overall,<br />
statins' expenditure rose 35.6% and 6.4% in 2006 and 2007 respectively, mainly from civil servant outpatients who are more<br />
likely to be prescribed with brand drugs, even among new patients. In all schemes, volume effect was positive, more people<br />
use statins, and each one used a larger quantity <strong>of</strong> drug in 2007 than in 2005. The price effect was negative. Drug mix effect<br />
indicated that there was a shift from higher to lower priced drugs in capitation scheme and from lower to higher priced<br />
original drugs in a fee-for-service scheme. Conclusion: There is a trend <strong>of</strong> more statins utilization but with a different pattern<br />
<strong>of</strong> drug prescribed between health schemes and possible drug over-prescribed in some patients. However, indication for<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
statin use was not studied, which needs to be considered. With high use <strong>of</strong> original drugs, effective policy initiative to<br />
promote generic drug use should be implemented in order to efficiently use the limited health care resources.<br />
No.943<br />
Author (s) : Pattarapanitchai, N., Tiensuwan, M., Riengrojpitak, S.<br />
Title : A retrospective study on homicidal autopsy cases at ramathibodi hospital in bangkok Thailand<br />
Source : Chiang Mai Journal <strong>of</strong> Science, Volume 37, Issue 2, May <strong>2010</strong>, Pages 282-292<br />
Document Type : Article<br />
Keywords : Homicide autopsy; Homicide methods; Homicide victims<br />
<strong>Abstract</strong> : Nowadays, the increasing incidence <strong>of</strong> homicide is worldwide and it is a matter <strong>of</strong> great concern all over the world including<br />
Thailand. The present study is aimed at a description <strong>of</strong> distributions <strong>of</strong> homicide and to find the association and risk ratio <strong>of</strong><br />
personal characteristics <strong>of</strong> homicide victim cases for Thai people. A 5-year retrospective review <strong>of</strong> personal and medicolegal<br />
autopsy records was conducted at the Forensic Medicine Division in the Department <strong>of</strong> Pathology, Faculty <strong>of</strong> Medicine,<br />
Ramathibodi Hospital, <strong>Mahidol</strong> <strong>University</strong>. A total <strong>of</strong> 218 cases <strong>of</strong> homicide victims autopsied during the study period spanning<br />
from January 2003 to December 2007 were identified. Data concerning age, gender, marital status, day and time <strong>of</strong> death,<br />
homicide methods, area <strong>of</strong> death, and the involvement <strong>of</strong> alcohol were analyzed. The results showed that homicidal victims<br />
were more likely to be male than female. The mean age <strong>of</strong> the victims was 32.14 years with standard deviation 13.67 years,<br />
although the most frequent age range was early adulthood (21-30 years). Blood alcohol was found in 102 cases (46.79%).<br />
Mean blood alcohol concentration (BAC) was 71.59 mg/dL with standard deviation 101.59 mg/dL. Approximately 35% <strong>of</strong> all<br />
homicide victims had BAC >50 mg/dL (the legal limit). Gun shot was the favorite homicide method used for both male and<br />
female victims (32.11%, 3.21%). Most <strong>of</strong> the crimes were occurred during the night (33.94%) and evening (26.15%). The rainy<br />
season and Sundays were the most common times for homicide. There was significant association between gender and<br />
homicide methods at p-value < 0.01. In addition, night time proved to be a significant factor in relation to the occurrence <strong>of</strong><br />
homicide at p-value < 0.01. Marital status associated homicide had more victims <strong>of</strong> the single and widowed status (OR = 1.63,<br />
2.29). The daytime <strong>of</strong> weekdays showed that the number <strong>of</strong> homicide victims was nearly 1.2 times <strong>of</strong> weekends (OR = 1.197).<br />
No.944<br />
Author (s) : Paz Diaz-Camacho, S.; Delgado-Vargas, F.; Willms, K.; del Carmen de la Cruz-Otero, M.; Guadalupe Rendon-Maldonado, J.;<br />
Robert, L.; Antuna, S.; Nawa, Y.<br />
Title : Intrahepatic growth and maturation <strong>of</strong> Gnathostoma turgidum in the natural definitive opossum host, Didelphis virginiana<br />
Source : PARASITOLOGY INTERNATIONAL, Volume: 59, Issue: 3, Pages: 338-343, DOI: 10.1016/j.parint.<strong>2010</strong>.04.004, Published: SEP <strong>2010</strong><br />
Document Type : Article<br />
Keywords : Gnathostoma turgidum; Advanced 3rd stage larvae; Opossum; Didelphis virginiana<br />
<strong>Abstract</strong> : Gnathostoma turgidum is a gastric nematode parasite <strong>of</strong> opossums found in the Americas. We recently found that G. turgidum<br />
juveniles appear in the liver <strong>of</strong> the opossums where they become mature adults and almost synchronously move to the<br />
stomach during certain months <strong>of</strong> the year, suggesting the importance <strong>of</strong> the liver for the growth and maturation <strong>of</strong> this<br />
species in the final hosts. In this study we attempted to detect G. turgidum larvae in the liver <strong>of</strong> opossums, Didelphis<br />
virginiana that are the natural final hosts. The results show that tiny (
<strong>Abstract</strong> : –<br />
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.946<br />
Author (s) : Peerapatdit, T.; Likidlilid, A.; Hobang, N.; Tresukosol, D.; Ratanamaneechat, S.<br />
Title : PARAOXONASE-2 GENE (PON2) S311C POLYMORPHISM ASSOCIATED WITH TYPE 2 DIABETIC MELLITUS AND CORONARY HEART<br />
DISEASE<br />
Source : ATHEROSCLEROSIS SUPPLEMENTS, Volume: 11, Issue: 2, Pages: 34-34, Meeting <strong>Abstract</strong>: P86, Published: JUN 10 <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.947<br />
Author (s) : Peng, LF.; Kapp, EA.; Fenyo, D.; Kwon, MS.; Jiang, P.; Wu, SF.; Jiang, Y.; Aguilar, MI.; Ahmed, N.; Baker, MS.; Cai, ZW.; Chen, YJ.;<br />
Van Chi, P.; Chung, MCM.; He, FC., Len, ACL.; Liao, PC.; Nakamura, K.; Ngai, SM.; Paik, YK.; Pan, TL.; Poon, TCW.; Salekdeh, GH.;<br />
Simpson, RJ.; Sirdeshmukh, R.; Srisomsap, C.; Svasti, J.; Tyan, YC.; Dreyer, FS.; McLauchlan, D.; Rawson, P.; Jordan, TW.<br />
Title : The Asia Oceania Human Proteome Organisation Membrane Proteomics Initiative. Preparation and characterisation <strong>of</strong> the<br />
carbonate-washed membrane standard<br />
Source : PROTEOMICS, Volume: 10, Issue: 22, Pages: 4142-4148, DOI: 10.1002/pmic.<strong>2010</strong>00126, Published: NOV <strong>2010</strong><br />
Document Type : Article<br />
Keywords : Asia Oceania Human Proteome Organisation; Cell Biology; Integral membrane proteins; Liver; Membrane proteomics<br />
<strong>Abstract</strong> : The Asia Oceania Human Proteome Organisation (AOHUPO) has embarked on a Membrane Proteomics Initiative with goals <strong>of</strong><br />
systematic comparison <strong>of</strong> strategies for analysis <strong>of</strong> membrane proteomes and discovery <strong>of</strong> membrane proteins. This<br />
multilaboratory project is based on the analysis <strong>of</strong> a subcellular fraction from mouse liver that contains endoplasmic<br />
reticulum and other organelles. In this study, we present the strategy used for the preparation and initial characterization <strong>of</strong><br />
the membrane sample, including validation that the carbonate-washing step enriches for integral and lipid-anchored<br />
membrane proteins. Analysis <strong>of</strong> 17 independent data sets from five types <strong>of</strong> proteomic workflows is in progress.<br />
No.948<br />
Author (s) : Pennell, D.; Porter, J.; Cappellini, M.; Chan, L. L.; El-Beshlawy, A.; Aydinok, Y.; Ibrahim, H.; Li, C. K.; Viprakasit, V.; Elalfy, M.;<br />
Kattamis, A.; Smith, G.; Habr, D.; Roubert, B.; Taher, A.<br />
Title : CONTINUED IMPROVEMENT IN CARDIAC T2*WITH DEFERASIROX TREATMENT OVER 2 YEARS: RESULTS FROM THE EXTENSION OF<br />
EPIC CARDIAC SUBSTUDY IN BETA-THALASSAEMIA PATIENTS WITH MYOCARDIAL SIDEROSIS<br />
Source : HAEMATOLOGICA-THE HEMATOLOGY JOURNAL, Volume: 95, Supplement: 2, Pages: 202-203, Meeting <strong>Abstract</strong>: 0498,<br />
Published: JUN <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.949<br />
Author (s) : Pennell, D.; Porter, J.; Cappellini, M.; Chan, L. L.; El-Beshlawy, A.; Aydinok, Y.; Ibrahim, H.; Li, C. K.; Viprakasit, V.; Elalfy, M.;<br />
Kattamis, A.; Smith, G.; Habr, D.; Roubert, B.; Taher, A.<br />
Title : DEFERASIROX EFFECTIVELY REMOVES CARDIAC IRON IN beta-THALASSAEMIA PATIENTS WITH MYOCARDIAL SIDEROSIS<br />
PREVIOUSLY CHELATED WITH DEFEROXAMINE MONOTHERAPY OR DEFEROXAMINE-DEFERIPRONE THERAPY<br />
Source : HAEMATOLOGICA-THE HEMATOLOGY JOURNAL, Volume: 95, Supplement: 2, Pages: 699-699, Meeting <strong>Abstract</strong>: 1805,<br />
Published: JUN <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.950<br />
Author (s) : Pennell, D.; Porter, JB.; Cappellini, MD.; Chan, LL.; El-Beshlawy, A.; Aydinok, Y.; Ibrahim, H.; Li, CK.; Viprakasit, V.; Elalfy, MS.;<br />
Kattamis, A.; Smith, G.; Habr, D.; Domokos, G.; Roubert, B.; Taher, A.<br />
348
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Continued Improvement and Normalization <strong>of</strong> Myocardial T2*In Patients with beta-thalassemia Major Treated With Deferasirox<br />
(Exjade (R)) for up to 3 Years<br />
Source : BLOOD, Volume: 116, Issue: 21, Pages: 1735-1736, Published: NOV 19 <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.951<br />
Author (s) : Peonim, V., Udnoon, J.<br />
Title : Left subclavian arterioesophageal fistula induced by chicken bone with upper gastrointestinal hemorrhage and unexpected<br />
death: Report <strong>of</strong> a case<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 11, November <strong>2010</strong>, Pages 1332-1335<br />
Document Type : Article<br />
Keywords : Chicken bone ingestion; Foreign body in the esophagus; Left subclavian arterioesophageal fistula; Unexpected<br />
death; Upper gastrointestinal hemorrhage<br />
<strong>Abstract</strong> : Left subclavian arterioesophageal fistula resulting from chicken bone ingestion is a rare occurrence. The authors report the<br />
death <strong>of</strong> a 42-year-old Thai female with mental retardation who presented to the hospital with severe hematemesis and<br />
arrested. Death occurred about 24 hours after laparotomy due to hypovolemic shock. Postmortem examination revealed a<br />
chicken bone embedded in middle part <strong>of</strong> esophagus with fistula between the esophagus and the left subclavian artery.<br />
No.952<br />
Author (s) : Perbangkhem, T., Polprasert, C.<br />
Title : Biomass production <strong>of</strong> papyrus (Cyperus papyrus) in constructed wetland treating low-strength domestic wastewater<br />
Source : Bioresource Technology, Volume 101, Issue 2, January <strong>2010</strong>, Pages 833-835<br />
Document Type : Article<br />
Keywords : Biomass productivity; Constructed wetland; Energy-capturing efficiency; Papyrus<br />
<strong>Abstract</strong> : In this study, the pilot-scale constructed wetlands were fed with low-strength domestic wastewater to investigate the energycapturing<br />
efficiency and plant productivity. Papyrus was a selected emergent macrophyte planted in the systems. The<br />
wastewater was intermittently fed to the systems, corresponding to the organic loading rates <strong>of</strong> 10, 16, 31, and 63 kg BOD/had.<br />
With abundant sunshine in the tropical-climate area, papyrus converted solar radiation to biomass <strong>of</strong> about 2200-3100 g<br />
dry weight/m2 from the two-month period <strong>of</strong> the experiments. Furthermore, the energy contents <strong>of</strong> papyrus are 16.2, 17.2,<br />
and 16.8 MJ/kg for culms, umbels, and total above-ground parts, respectively. From the plant productivity and the energy<br />
contents <strong>of</strong> papyrus obtained from this study, the energy capturing efficiencies can be estimated to be in the range <strong>of</strong> 4.4-<br />
6.0%, which are relatively high, compared with those <strong>of</strong> other plants. © 2009 Elsevier Ltd. All rights reserved.<br />
No.953<br />
Author (s) : Permpikul, C., Tongyoo, S., Ratanarat, R., Wilachone, W., Poompichet, A.<br />
Title : Impact <strong>of</strong> septic shock hemodynamic resuscitation guidelines on rapid early volume replacement and reduced mortality.<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand = Chotmaihet thangphaet, Volume 93 Suppl 1, January <strong>2010</strong>, Pages S102-109<br />
Document Type : Article<br />
Keywords : adult, aged, APACHE, article, female, fluid therapy, hospital department, human, intensive care unit, length <strong>of</strong><br />
stay, male, methodology, middle aged, mortality, practice guideline, resuscitation, retrospective study, septic<br />
shock, standard, survival, time, treatment outcome; Adult, Aged, Aged, 80 and over, APACHE, Female, Fluid Therapy, Hospital<br />
Departments, Hospital Mortality, Humans, Intensive Care Units, Length <strong>of</strong> Stay, Male, Middle Aged, Outcome and Process<br />
Assessment (Health Care), Practice Guidelines as Topic, Resuscitation, Retrospective Studies, Shock, Septic, Survival<br />
Analysis, Time Factors, Treatment Outcome<br />
<strong>Abstract</strong> : BACKGROUND: Septic shock is one <strong>of</strong> the most serious conditions associated with high mortality. We recently developed a<br />
modified septic shock management guideline focusing on rapid restoration <strong>of</strong> hemodynamics by using clinical endpoint. Our<br />
aim was to analyze patients' outcomes following the guideline implementation. MATERIAL AND METHOD: A retrospective<br />
review <strong>of</strong> hemodynamic data sheet and clinical outcomes <strong>of</strong> patients admitted to medical ICU and medical Wards and during<br />
June 2004 and February 2006. RESULTS: One hundred and four patients' records were retrieved. The patients' mean age was<br />
62.5 +/- 18.6 year. Their mean APACHE II score were 24.9 +/- 6.7 and the overall mortality was 59%. Sixty eight patients<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
(65.4%) underwent guideline directed therapy (guideline group). The guideline group received higher volume resuscitation<br />
from the first hour <strong>of</strong> resuscitation (1,016.3 + 675.0 ml vs. 521.4 + 359.2 ml, p < 0.001) to the forty eighth hour (10,096.9 +/-<br />
3,256.1 ml vs. 8,067.3 +/- 2,591.9 ml, p = 0.006). More <strong>of</strong> them achieved the therapeutic goal within 6 hours (86.8% vs. 44.4%,<br />
p < 0.001) and their hospital mortality was lower (41.2% vs. 69.4%, p = 0.008). When analyzing differences between those<br />
who survived and those who died, more <strong>of</strong> the surviving patients underwent guideline directed treatment (79.5% vs. 55%, p =<br />
0.012). They received higher volume replacement from the first hour to the end <strong>of</strong> the twelfth hour (first hour 1,098.0 +/-<br />
723.0 vs. 660.9 +/- 478.9 ml, p < 0.001; the end <strong>of</strong> the twelfth hour 3,746.6 +/- 1,799 vs. 3,014.1 +/- 1,579.9 ml, p = 0.038)<br />
and more <strong>of</strong> them achieved the therapeutic goal within 6 hours (95.5% vs. 55%, p < 0.001). Multivariate analysis <strong>of</strong> factors<br />
associated with mortality disclosed APACHE II score, volume resuscitation more than 800 ml in the first hour and achievement<br />
<strong>of</strong> the therapeutic goal within 6 hours. CONCLUSION: Implementation <strong>of</strong> our modified septic shock guideline is associated<br />
with rapid initial volume replacement, prompt achievement <strong>of</strong> therapeutic goal and improved outcomes. Volume<br />
resuscitation greater than 800 ml in the first hour is associated with better survival.<br />
No.954<br />
Author (s) : Permpikul, P.; Senawong, S.; Panchavinnin, W.<br />
Title : MUKLTIPLEX PCR OF PREVIOUS DONATION REPOSITORY SAMPLES OF POSITIVE HBV NAT DONATIONS: EXPERIENCE FROM<br />
THAILAND<br />
Source : VOX SANGUINIS, Volume: 99, Supplement: 1, Pages: 296-296, Published: JUL <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.955<br />
Author (s) : Permpongkosol, S., Bella, A.J., Suntisevee, S., Leenanupunth, C., Stoller, M.L.<br />
Title : Laparoscopic excision <strong>of</strong> urachal cysts in elderly men and woman following pregnancy<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 1, January <strong>2010</strong>, Pages 132-136<br />
Document Type : Article<br />
Keywords : Laparoscopy; Remnant; Surgery; Urachal cyst<br />
<strong>Abstract</strong> : Background and Objective: Persistent urachus rarely presents in the aging male or during pregnancy. The authors report their<br />
experience with the laparoscopic excision <strong>of</strong> urachal cysts in two elderly men with significant co-morbidities and following<br />
pregnancy in a 32-year old female. Material and Method: The two male patients (65 and 70 years old, respectively) presented<br />
with a lower abdominal mass and umbilical discharge, while persistent urachus was identified incidentally during pregnancy;<br />
patients were managed with laparoscopic excision at 4 weeks, 6 weeks, and one year after diagnosis, respectively. Using 3<br />
port accesses, the urachus and medial umbilical ligament were clipped and divided. In 2 cases, specimens were separated<br />
from the bladder dome with a bladder cuff. In one patient, an additional port was required to facilitate intracorporeal<br />
freehand suturing <strong>of</strong> the bladder defect. Results: All procedures were completed successfully via laparoscopy. No<br />
intraoperative or postoperative complications were reported. Operative time ranged from 120,180 and 160 minutes,<br />
respectively; in-hospital convalescence was 1, 7, and 6 days, respectively. Pathological evaluation revealed a benign urachal<br />
remnant in each case. Conclusion: Laparoscopic excision <strong>of</strong> urachal cysts in the aging male or following pregnancy is safe and<br />
effective.<br />
No.956<br />
Author (s) : Permpongkosol, S., Nontakaew, K., Liranupat, C.<br />
Title : Laparoendoscopic Single-Site (LESS) nephrectomy in a patient undergoing continuous ambulatory peritoneal dialysis (CAPD)<br />
Source : <strong>International</strong> Braz J Urol, Volume 36, Issue 6, December <strong>2010</strong>, Page 781<br />
Document Type : Note<br />
Keywords : –<br />
<strong>Abstract</strong> : Purpose: To assess the feasibility, safety, and outcomes <strong>of</strong> transumbilical single port laparoscopic nephrectomy in a patient<br />
undergoing continuous ambulatory peritoneal dialysis (CAPD) treatment. Materials and Methods: A patient was a 62-year-old<br />
woman who had been using CAPD for 4 months because <strong>of</strong> end-stage renal disease (ESRD) secondary to diabetic<br />
nephropathy. She was referred to the urology clinic, due to suffering from the left pyonephrosis and non-functioning kidney<br />
with a proximal ureteric stone. Then she underwent percutaneous nephrostomy to drain the pus. After this resolved, she<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
underwent LESS nephrectomy. Medical records were reviewed, and laboratory values and outcomes were analyzed. Results:<br />
The procedure was successfully completed without conversion to conventional laparoscopic or open surgery. Operative time<br />
was 160 minutes. Moreover, the specimen can be easily and rapidly extracted through the TriPort without the use <strong>of</strong> an<br />
additional entrapment bag. Estimated blood loss was 200 mL and pain scale was 0-1, with no morphine requirements. On the<br />
same day <strong>of</strong> the operative procedure, a double-lumen hemodialysis (HD) catheter was introduced for postoperative HD. The<br />
patient underwent hemodialysis treatment via a subclavian catheter after the operation and she was discharged home on the<br />
sixth postoperative day with a functioning CAPD catheter, with no further problems. After 2 weeks, the patient switched from<br />
HD to CAPD without complications. Pathological analysis revealed chronic pyelonephritis. Postoperative hematocrit, blood<br />
urea nitrogen, and creatinine were 39.3%, 22 mg/dL, 2.3 mg/dL respectively. The scars receded into the umbilicus and were<br />
hardly visible. Conclusion: LESS nephrectomy is a feasible technique with advantages <strong>of</strong> less pain, shortened convalescence,<br />
improved cosmesis, and absence <strong>of</strong> wound complications.<br />
No.957<br />
Author (s) : Permpongkosol, S., Tantirangsee, N., Ratana-Olarn, K.<br />
Title : Treatment <strong>of</strong> 161 men with symptomatic late onset hypogonadism with long-acting parenteral testosterone undecanoate:<br />
Effects on body composition, lipids, and psychosexual complaints<br />
Source : Journal <strong>of</strong> Sexual Medicine, Volume 7, Issue 11, November <strong>2010</strong>, Pages 3765-3774<br />
Document Type : Article<br />
Keywords : <strong>International</strong> Index <strong>of</strong> Erectile Function; Late-Onset Hypogonadism; Prostate Safety; Sex Hormone Binding<br />
Globulin; Testosterone Deficiency Syndrome; Testosterone Undecanoate; Thai; Waist Circumference<br />
<strong>Abstract</strong> : Introduction. Elderly men may suffer from late-onset hypogonadism (LOH). The long-term effects <strong>of</strong> long-acting testosterone<br />
undecanoate (TU) in a large number <strong>of</strong> LOH men have not yet been reported. Aims. We analyzed the effects <strong>of</strong> normalization<br />
<strong>of</strong> plasma testosterone (T) in LOH men. Methods. The records <strong>of</strong> 161 men with LOH (baseline T < 300 ng/dL) were reviewed<br />
and 100 men had used parenteral TU for >12 months. The mean duration <strong>of</strong> treatment was 90.6 weeks (54 to 150 weeks).<br />
Main Outcome Measures. Body mass index (BMI), waist circumference, percentage body fat, total cholesterol, high-density<br />
lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL) cholesterol, triglycerides, prostate-specific antigen (PSA), and<br />
hematocrit were measured. Further the Aging Male Symptoms' scale (AMS) and the <strong>International</strong> Index <strong>of</strong> Erectile Function<br />
(IIEF-5 and 15) were scored. Results. T therapy was associated with a significant decline in waist circumference (P = 0.028) and<br />
percentage body fat (P < 0.001), but no change <strong>of</strong> BMI. Total cholesterol and LDL cholesterol declined significantly (P = 0.005<br />
and P = 0.024, respectively), with no significant changes <strong>of</strong> HDL cholesterol and triglycerides. The scores <strong>of</strong> sub-scales <strong>of</strong> AMS<br />
(psychological, somotovegetative and sexual factors) decreased (P = 0.044, P = 0.200 and P = 0.071, respectively). The mean<br />
IIEF-5 (P = 0.011) and IIEF-15 scores (P = 0.021) improved significantly. Erectile function domain, orgasmic function domain,<br />
sexual desire domain, intercourse satisfaction domain, and overall satisfaction domain improved. Median PSA rose from 0.95<br />
(0.640; 1.558) ng/mL to 1.480 (1.015; 2.275) ng/mL (P < 0.001), with 11 patients >4 ng/mL (4.01-13.21). On biopsy there was no<br />
evidence for malignancy. The mean hematocrit level increased significantly from 42.3 ± 3.4% to 47.1 ± 3.8%. Conclusions.<br />
Normalizing serum T in men with LOH resulted in improvement <strong>of</strong> the metabolic syndrome, mood and sexual functions and<br />
appeared acceptably safe. © <strong>2010</strong> <strong>International</strong> Society for Sexual Medicine.<br />
No.958<br />
Author (s) : Permpongkosol, S.; Rata-Olarn, K.<br />
Title : ORAL TESTOSTERONE USE BEHAVIOR AMONG 254 SYMPTOMATIC LATE-ONSET HYPOGONADAL MEN<br />
Source : JOURNAL OF SEXUAL MEDICINE, Volume: 7, Special Issue: SI, Supplement: 4, Pages: 221-221, Meeting <strong>Abstract</strong>: 232, Published:<br />
SEP <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.959<br />
Author (s) : Permpongkosol, S.; Tantirangsee, N.; Liranupat, C.<br />
Title : SINGLE PORT BOWEL RESECTION AND ANASTOMOSIS FOR ILEAL CONDUIT: AN EXPERIMENT TECHNIQUE IN A PORCINE MODEL<br />
Source : JOURNAL OF ENDOUROLOGY, Volume: 24, Supplement: 1, Pages: A383-A383, Published: SEP <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
351
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<strong>Abstract</strong> : –<br />
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No.960<br />
Author (s) : Permpongkosol, S.; Veraasertniyom, O.; Tongprsdit, S.; Pettong, T.; Putthapiban, Y.; Ratana-olarn, K.<br />
Title : THE RELATIONSHIP BETWEEN ANDROGEN RECEPTOR GENE CAG REPEATS LENGTH AND LONG TERM OUTCOME OF<br />
INTRAMUSCULAR TESTOSTERONE UNDECANOATE THERAPY IN 190 THAI LATE ONSET HYPOGONADAL MEN<br />
Source : INTERNATIONAL JOURNAL OF UROLOGY, Volume: 17, Supplement: 1, Pages: A100-A100, Published: AUG <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.961<br />
Author (s) : Permpongkosol, S.<br />
Title : An improvement <strong>of</strong> both voiding and sexual dysfunction in 313 Thai men with lower urinary tract symptoms after an<br />
uroselective alpha 1-blocker treatment<br />
Source : INTERNATIONAL JOURNAL OF UROLOGY, Volume: 17, Supplement: 1, Pages: A332-A332, Published: AUG <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.962<br />
Author (s) : Permpongkosol, S.; Chaimuangraj, S.; Piyasoonthawon, N.; Leenanupunth, C.<br />
Title : Laparo-endoscopic single site (LESS) decortication <strong>of</strong> symptomatic renal cysts with sils ports and roticulator instruments<br />
Source : INTERNATIONAL JOURNAL OF UROLOGY, Volume: 17, Supplement: 1, Pages: A398-A398, Published: AUG <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.963<br />
Author (s) : Permpongkosol, S.; Chanprasopon, P.; Leenanupunth, C.; Wachira, K.; Chaimuangraj, S.; Ratana-olarn, K.<br />
Title : Long-term study <strong>of</strong> quality <strong>of</strong> life <strong>of</strong> 186 living kidney donors: an evaluation by using the 36-item health questionnaire survey<br />
Source : INTERNATIONAL JOURNAL OF UROLOGY, Volume: 17, Supplement: 1, Pages: A331-A332, Published: AUG <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.964<br />
Author (s) : Permpongkosol, S.; Phanpradit, O.; Leenanupunth, C.<br />
Title : Laparo-endoscopic single site (LESS) management <strong>of</strong> benign kidney diseases: evaluation <strong>of</strong> complications<br />
Source : INTERNATIONAL JOURNAL OF UROLOGY, Volume: 17, Supplement: 1, Pages: A332-A333, Published: AUG <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.965<br />
Author (s) : Permpongkosol, S.; Tantirangsee, N.; Chaimuangraj, S.; Leenanupunt, C.<br />
Title : Hybrid transvaginal laparoendoscopic single site (LESS) nephrectomy in a porcine model<br />
Source : INTERNATIONAL JOURNAL OF UROLOGY, Volume: 17, Supplement: 1, Pages: A399-A399, Published: AUG <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
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No.966<br />
Author (s) : Permpongkosol, S.; Tantirangsee, N.; Ratana-olarn, K.<br />
Title : The relationship between androgen receptor gene CAG repeats length and long term outcome <strong>of</strong> intramuscular testosterone<br />
undecanoate therapy in 183 Thai late onset hypogonadal men<br />
Source : INTERNATIONAL JOURNAL OF UROLOGY, Volume: 17, Supplement: 1, Pages: A330-A331, Published: AUG <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.967<br />
Author (s) : Permpongkosol, S.; Ungnhakorn, P.; Leenanupunth, C.<br />
Title : Laparoendoscopic single site ureteral reimplantation as a treatment <strong>of</strong> the single ectopic ureter<br />
Source : INTERNATIONAL JOURNAL OF UROLOGY, Volume: 17, Supplement: 1, Pages: A398-A399, Published: AUG <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.968<br />
Author (s) : Permpoon, R., Thanaphum, S.<br />
Title : Isolation and characterization <strong>of</strong> oligomerization domain I and II coding regions <strong>of</strong> doublesex genes in agricultural fruit flies<br />
(Diptera: Tephritidae)<br />
Source : European Journal <strong>of</strong> Entomology, Volume 107, Issue 1, <strong>2010</strong>, Pages 121-126<br />
Document Type : Article<br />
Keywords : Bactrocera; Doublesex genes; OD1 and OD2 domains; Phylogenetic relationships<br />
<strong>Abstract</strong> : Bactrocera fruit flies are ranked among the most destructive pests <strong>of</strong> the worldwide fruit and vegetable trades. Coding regions<br />
<strong>of</strong> two oligomerization domains within doublesex (dsx) genes were determined in Bactrocera dorsalis (oriental fruit fly) and B.<br />
correcta (guava fruit fly). Resulting sequences revealed a high degree <strong>of</strong> similarity at both nucleotide and putative amino acid<br />
levels in the genus Bactrocera. Conservation <strong>of</strong> the DNA-binding DM motif and several known molecular features within the<br />
domains suggest a presence <strong>of</strong> strong purifying selection on the DSX proteins. Topology <strong>of</strong> the phylogenetic gene trees and<br />
deduced amino acid substitution patterns suggest that the coding region sequences <strong>of</strong> the two domains are diversified in<br />
concert parallel with the species differentiation. © 2003 Institute <strong>of</strong> Entomology.<br />
No.969<br />
Author (s) : Permpoonputtana, K.; Govitrapong, P.<br />
Title : INVOLVEMENT OF MITOGEN-ACTIVATED PROTEIN KINASE IN METHAMPHETAMINE-INDUCED NEUROINFLAMMATION OF HUMAN<br />
NEUROBLASTOMA SH-SY5Y CELLS<br />
Source : JOURNAL OF NEUROCHEMISTRY, Volume: 115, Supplement: 1, Pages: 82-83, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.970<br />
Author (s) : Perrine, S.P., Castaneda, S.A., Chui, D.H.K., Faller, D.V., Berenson, R.J., Siritanaratku, N., Fucharoen, S.<br />
Title : Fetal globin gene inducers: Novel agents and new potential<br />
Source : Annals <strong>of</strong> the New York Academy <strong>of</strong> Sciences, Volume 1202, August <strong>2010</strong>, Pages 158-164<br />
Document Type : Conference Paper<br />
Keywords : erythropoiesis; fetal globin; quantitative trait loci; short chain fatty acids; thalassemia<br />
<strong>Abstract</strong> : Inducing expression <strong>of</strong> endogenous fetal globin (γ-globin) gene expression to 60-70% <strong>of</strong> alpha globin synthesis produces βthalassemia<br />
trait globin synthetic ratios and can reduce anemia to a mild level. Several classes <strong>of</strong> therapeutics have induced<br />
γ-globin expression in beta-thalassemia patients and subsequently raised total hemoglobin levels, demonstrating pro<strong>of</strong>-<strong>of</strong>concept<br />
<strong>of</strong> the approach. Butyrate treatment eliminated transfusion requirements in formerly transfusion-dependent patients<br />
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with treatment for as long as seven years. However, prior generation inducers were not readily applicable for widespread use.<br />
Currently, a novel oral dual-action therapeutic, sodium 2,2-dimethylbutyrate, is in clinical trials, an oral decitabine formulation<br />
is under development, and agents with complementary mechanisms <strong>of</strong> action can be applied in combined regimens.<br />
Identification <strong>of</strong> three major genetic trait loci which modulate clinical severity provides avenues for developing tailored<br />
regimens. These refinements <strong>of</strong>fer renewed potential to apply fetal globin induction as a treatment approach in patientfriendly<br />
regimens that can be used worldwide. © <strong>2010</strong> New York Academy <strong>of</strong> Sciences.<br />
No.971<br />
Author (s) : Peters, P.J., Stringer, J., McConnell, M.S., Kiarie, J., Ratanasuwan, W., Intalapaporn, P., Potter, D., Mutsotso, W., Zulu, I., Borkowf,<br />
C.B., Bolu, O., Brooks, J.T., Weidle, P.J.<br />
Title : Nevirapine-associated hepatotoxicity was not predicted by CD4 count ≥250 cells/μL among women in Zambia, Thailand and<br />
Kenya<br />
Source : HIV Medicine, Volume 11, Issue 10, November <strong>2010</strong>, Pages 650-660<br />
Document Type : Article<br />
Keywords : Antiretroviral therapy; Drug hypersensitivity; Drug-induced hepatitis; HIV; Nevirapine; Skin rash<br />
<strong>Abstract</strong> : Objective The aim <strong>of</strong> the study was to determine risk factors for developing severe hepatotoxicity (grade 3 or 4<br />
hepatotoxicity) and rash-associated hepatotoxicity (rash with ≥grade 2 hepatotoxicity) among women initiating nevirapinebased<br />
antiretroviral therapy (ART).Methods The Non-Nucleoside Reverse Transcriptase Inhibitor Response Study was a<br />
prospective cohort study carried out in Zambia, Thailand and Kenya. Between May 2005 and January 2007, we enrolled<br />
antiretroviral-naïve HIV-infected women initiating nevirapine-based ART. At enrolment and at weeks 2, 4, 8, 16 and 24,<br />
participants had serum alanine transferase (ALT) and aspartate transaminase (AST) measured and were evaluated clinically for<br />
hepatitis and rash.Results Nevirapine-based ART was initiated in 820 women and baseline ALT or AST results were abnormal<br />
(≥grade 1) in 113 (14%) women. After initiating nevirapine-based ART, severe hepatotoxicity occurred in 41 (5%) women and<br />
rash-associated hepatotoxicity occurred in 27 (3%) women. In a multivariate logistic regression model, severe hepatotoxicity<br />
and rash-associated hepatotoxicity were both associated with baseline abnormal (≥grade 1) ALT or AST results, but not with a<br />
baseline CD4 cell count ≥250 cells/μL. Three participants (0.4%) died with symptoms suggestive <strong>of</strong> fatal hepatotoxicity; all<br />
three women had baseline CD4 count
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
categorized as being infection related (NADC-IR) and unrelated (NADC-IUR). Results. A total <strong>of</strong> 617 patients were included in<br />
this study: 215 cancer cases and 402 controls from 13 sites. The majority <strong>of</strong> cancer cases were male (71%). The mean age<br />
(SD) for cases was 39 (10.6), 46 (11.5) and 44 (13.7) for ADCs, NADC-IURs and NADCs-IR, respectively. The majority (66%) <strong>of</strong><br />
cancers were ADCs (16% Kaposi sarcoma, 40% non-Hodgkin's lymphoma, and 9% cervical cancer). The most common NADCs<br />
were lung (6%), breast (5%) and hepatocellular carcinoma and Hodgkin's lymphoma (2% each). There were also three (1.4%)<br />
cases <strong>of</strong> leiomyosarcoma reported in this study. In multivariate analyses, individuals with CD4 counts above 200 cells/mm3<br />
were approximately 80% less likely to be diagnosed with an ADC (p < 0.001). Older age (OR: 1.39, p = 0.001) and currently<br />
not receiving antiretroviral treatment (OR: 0.29, p = 0.006) were independent predictors <strong>of</strong> NADCs overall, and similarly for<br />
NADCs-IUR. Lower CD4 cell count and higher CDC stage (p = 0.041) were the only independent predictors <strong>of</strong> NADCs-IR.<br />
Conclusions. The spectrum <strong>of</strong> cancer diagnoses in the Asia region currently does not appear dissimilar to that observed in<br />
non-Asian HIV populations. One interesting finding was the cases <strong>of</strong> leiomyosarcoma, a smooth-muscle tumour, usually seen<br />
in children and young adults with AIDS, yet overall quite rare. Further detailed studies are required to better describe the<br />
range <strong>of</strong> cancers in this region, and to help guide the development <strong>of</strong> screening programmes. © <strong>2010</strong> Petoumenos et al;<br />
licensee BioMed Central Ltd.<br />
No.973<br />
Author (s) : Pewnim, K., Ketpichainarong, W., Ruenwongsa, P.<br />
Title : Bringing science to community: A STS-based learning unit on biocontrol for secondary school students<br />
Source : <strong>International</strong> Journal <strong>of</strong> Learning, Volume 17, Issue 4, <strong>2010</strong>, Pages 29-44<br />
Document Type : Article<br />
Keywords : Biocontrol; Environmental awareness; Local wisdom; Secondary student; STS approach<br />
<strong>Abstract</strong> : This study aimed to develop a STS-based learning unit on biocontrol for secondary school students to enhance their<br />
understanding <strong>of</strong> biocontrol as well as raise awareness in their local environment. They should be able to connect science in<br />
classroom to local problems in their community. This study focused on the collaboration between students, school teachers,<br />
and local sages in supporting the authentic learning <strong>of</strong> students by using biocontrol experiment as a case study. The teachers<br />
used STS approach (Science, Technology and Society) to construct the novel lesson plan on biocontrol by using local<br />
materials from school vicinity and/or student's farm. The students learned basic techniques in controlling plant pathogens<br />
and insect pests by biological agents, both from school and from visiting the local farm and discussing with local experts.<br />
Teachers then encouraged them to design and conduct their own projects to solve problems on plants, fruits and vegetables<br />
diseases in their own area. Teachers supported them with certain laboratory materials and equipment as well as necessary<br />
textbooks and journals. Four instruments were used to assess students' achievement: pretest and posttest, questionnaire on<br />
awareness, semi-structured interview, and classroom observation. The results showed a significant increase in students'<br />
understanding <strong>of</strong> biocontrol concepts and awareness <strong>of</strong> the local problem. The students applied and integrated the<br />
knowledge learned to help solving problem on plants' diseases in their community. © Common Ground, Kanrawee Pewnim,<br />
Watcharee Ketpichainarong*, Pintip Ruenwongsa.<br />
No.974<br />
Author (s) : Phaiboon, S.<br />
Title : RF macro-cell prediction using fuzzy logic: Case study in Bangkok City –Thailand<br />
Source : <strong>International</strong> Conference on Communications and Information Technology - Proceedings, <strong>2010</strong>, Pages 105-109<br />
Document Type : Conference Paper<br />
Keywords : Fuzzy logic approximation; Macro cell path loss; The 1 km radius<br />
<strong>Abstract</strong> : This paper proposes an simple method to predict macro cell design using fuzzy logic approximation. The concept <strong>of</strong> Fuzzy<br />
logic was applied to predict path loss at 1 km interception point. The input fuzzy sets were classified into three sets namely,<br />
percent <strong>of</strong> buildings, houses and tree within 1 km radius area. A inference engine was then applied via linguistic rules which<br />
were trained by conventional point to point model in realistic environment. To check the proposed model, we compared the<br />
fuzzy prediction with other areas. The results shown that the fuzzy logic approximation provided an accuracy prediction and<br />
a user friendly prediction.<br />
No.975<br />
Author (s) : Phaiboon, S., Phokharatkul, P.<br />
Title : Comparison between Xia and Walfisch-Ikegami models for low-rise building path loss prediction<br />
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Source : IEEE Region 10 Annual <strong>International</strong> Conference, Proceedings/TENCON, <strong>2010</strong>, Article number 5686558, Pages 889-892<br />
Document Type : Conference Paper<br />
Keywords : Low rise buildings; Path loss measurements; WI model; Xia model<br />
<strong>Abstract</strong> : This paper presents path loss prediction on 2-D aerial images taken from the actual areas. The original Xia path loss model<br />
and the Walfisch-Ikegami model were applied for predicting in low rise building area. In case <strong>of</strong> the Xia model for non line <strong>of</strong><br />
sight (NLOS), it requires a set <strong>of</strong> model parameters such as distance from the last ro<strong>of</strong> top to receiver, average building height,<br />
and antenna heights. While the Walfisch-Ikegami (WI) model requires road parameters such as road width and road<br />
orientation. We performed measurements at frequencies <strong>of</strong> 900 and 1800 MHz for comparison between the two approaches.<br />
From the results it is found that the Xia model provides good agreement, while the WI model provides good agreement<br />
especially when the receiver is on the street between two buildings. © <strong>2010</strong> IEEE.<br />
No.976<br />
Author (s) : Phanuphak, N., Lolekha, R., Chokephaibulkit, K., Voramongkol, N., Boonsuk, S., Limtrakul, A., Limpanyalert, P., Chasombat, S.,<br />
Thanprasertsuk, S., Leechawengwong, M.<br />
Title : Thai national guidelines for the prevention <strong>of</strong> motherto-child transmission <strong>of</strong> HIV: March <strong>2010</strong><br />
Source : Asian Biomedicine, Volume 4, Issue 4, August <strong>2010</strong>, Pages 529-540<br />
Document Type : Review<br />
Keywords : HIV; Mother-to-child transmission; Thai guidelines<br />
<strong>Abstract</strong> : Thailand has been one <strong>of</strong> the leading developing countries to implement a national program to prevent mother-to-child<br />
transmission (MTCT) <strong>of</strong> HIV. Although the recent transmission rate has been low, the goal is to eliminate MTCT altogether. The<br />
Thai National HIV Guidelines Working Group issued treatment guidelines to prevent MTCT in Thailand in March <strong>2010</strong>. These<br />
guidelines will be implemented nationwide within a year. The most important aspects <strong>of</strong> these new guidelines are as follows:<br />
Treatment in HIV-infected pregnant women who have not been on antiretroviral treatment prior to pregnancy. Antepartum<br />
treatment is recommended for all pregnant women regardless <strong>of</strong> CD4 count with highly active antiretroviral therapy (HAART)<br />
containing zidovudine (AZT) + lamivudine (3TC) + lopinavir/ritonavir (LPV/r). Treatment should be started immediately<br />
irrespective <strong>of</strong> gestational age in women with CD4 count ≤350 cells/ mm 3, and as early as 14 weeks <strong>of</strong> gestation in those<br />
with CD4 count >350 cells/mm3. After delivery, women with baseline CD4 count ≤350 cells/mm3 are referred for longterm<br />
care and HAART according to the National Adult HIV Treatment and Care Guidelines <strong>2010</strong>. Women with CD4 count<br />
>350 cells/mm3 do not need HAART and can stop all drugs after delivery. The treatment in infants includes AZT syrup for<br />
four weeks and exclusive formula feeding. Treatment in HIV-infected pregnant women who conceive while on HAART.<br />
Women who are stable on HAART should continue the treatment during the whole period <strong>of</strong> pregnancy. Those who are<br />
taking efavirenz (EFV) and present during the first trimester should have EFV switched to another drug. Whenever possible,<br />
AZT should be used during pregnancy. Treatment in infants is similar to the above scenario. Treatment in women who<br />
present in labor without antenatal care. Single-dose nevirapine (SD-NVP) 200 mg must be given immediately along with oral<br />
AZT 300 mg every three hours until delivery, or oral AZT 600 mg given as a single dose. The tail therapy <strong>of</strong> AZT + 3TC + LPV/r<br />
for four weeks should be given unless these women have a CD4 count <strong>of</strong> ≤350 cells/mm 3 and therefore require life-long<br />
HAART. SD-NVP should not be given if the women are to deliver within two hours. The infants in this situation should receive<br />
AZT + 3TC + NVP for four weeks. Treatment during delivery and mode <strong>of</strong> delivery. During labor, oral AZT 300 mg every three<br />
hours or oral AZT 600 mg given as a single dose is recommended regardless <strong>of</strong> antepartum antiretroviral (ARV) regimen or the<br />
woman's history <strong>of</strong> AZT resistance. Elective caesarean section is suggested in women who did not receive HAART (including<br />
those without antenatal care), received HAART for less than four weeks prior to delivery, had poor adherence, or had<br />
incomplete viral suppression at 36 weeks <strong>of</strong> gestation.<br />
No.977<br />
Author (s) : Phanwichien, K., Pradermwong, A., Keenan, H.E., Masawang, K., Lauhachinda, N., Songsasen, A., Sakultantimetha, A.,<br />
Bangkedphol, S., Gaines, A.F.<br />
Title : The ecological complexity <strong>of</strong> the Thai-Laos Mekong River: III. Health status <strong>of</strong> Mekong catfish and cyprinids, evidence <strong>of</strong><br />
bioaccumulative effects<br />
Source : Journal <strong>of</strong> Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering, Volume<br />
45, Issue 13, November <strong>2010</strong>, Pages 1681-1688<br />
Document Type : Article<br />
Keywords : bioaccumulation; catfish; cyrinids; Histopathology; Mekong River; metals; PAHs<br />
356
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : Histopathology <strong>of</strong> fish organs was used as biomarkers <strong>of</strong> toxicity from environmental pollutants. A total <strong>of</strong> 117 fishes<br />
comprising <strong>of</strong> 52 cyprinids and 65 catfishes were randomly collected from the Mekong River from 5 stations: Chiang Rai, Loei,<br />
Nongkhai, Nakhon Phanom and Ubonratchathani. The health <strong>of</strong> the fish collected in December and April, winter and summer<br />
respectively, was evaluated. All fish from the 5 stations developed pathologic lesions with the same characteristics in their<br />
livers, kidneys and spleen. In the liver, there was vacuolation <strong>of</strong> hepatocytes, accumulation <strong>of</strong> brownish-green granules in the<br />
cytoplasm <strong>of</strong> hepatocytes, necrosis <strong>of</strong> hepatocytes, granuloma formation and angiogenesis. Kidney lesions consisted <strong>of</strong><br />
glomerular degeneration, necrosis and focal hyperplasia <strong>of</strong> renal tubules. In the spleen, there were haemorrhage,<br />
melanomacrophage centre infiltration and necrosis <strong>of</strong> the red pulp and white pulp. The pathologic severity <strong>of</strong> the catfish was<br />
found to be more severe than in the cyprinids and the catfish collected in summer were less healthy than the catfish<br />
collected in the winter. These histopathological appearances might arise from the fish feeding on the benthos and thereby<br />
accumulating toxic pollutants in their organs. The activities <strong>of</strong> the serum enzymes, Glutamic Oxalacetic Transaminase (GOT)<br />
and Glutamic Pyruvic Transaminase (GPT), were markedly increased, indicating detoxification activity. The highest activity <strong>of</strong><br />
GOT found in the cyprinids from Chiang Rai 365.30 U/L whereas in the catfish from Nakhon Phanom the activity was 300.73<br />
U/L. The highest GPT activity found in the cyprinids from Nakhon Phanom was 203.23 U/L where as in the catfish from the<br />
same station was GPT 389.77 U/L. According to the results from this study, catfish collected from Chiang Rai, Nakhon Phanom<br />
and Ubonratchathani showed more severe pathological changes than catfish from the other stations. Fish organs and river<br />
water were analysed for Polyaromatic Hydrocarbons (PAHs) and metals. The fish organs showed bioaccumulation <strong>of</strong> these<br />
toxic pollutants. BioConcentration Factors (BCFs) were calculated. Therefore an attempt is made to correlate these findings to<br />
the Mekong study in general. Copyright © Taylor & Francis Group, LLC.<br />
No.978<br />
Author (s) : Phasukkijwatana, N., Kunhapan, B., Stankovich, J., Chuenkongkaew, W.L., Thomson, R., Thornton, T., Bahlo, M., Mushiroda, T.,<br />
Nakamura, Y., Mahasirimongkol, S., Tun, A.W., Srisawat, C., Limwongse, C., Peerapittayamongkol, C., Sura, T., Suthammarak, W.,<br />
Lertrit, P.<br />
Title : Genome-wide linkage scan and association study <strong>of</strong> PARL to the expression <strong>of</strong> LHON families in Thailand<br />
Source : Human Genetics, Volume 128, Issue 1, July <strong>2010</strong>, Pages 39-49<br />
Document Type : Article<br />
Keywords : cytochrome c, mit<strong>of</strong>usin 1, translocase <strong>of</strong> outer mitochondrial membrane 20, metalloproteinase, mitochondrial protein, PARL<br />
protein, human; allele, apoptosis, article, cell death, chromosome 12, chromosome 13, chromosome 18, chromosome<br />
3, chromosome 3q, data base, family, female, gene expression, genetic association, genetic<br />
linkage, genotype, heterozygote, human, Leber hereditary optic neuropathy, major clinical study, male, microsatellite<br />
marker, nerve degeneration, pedigree, priority journal, protein degradation, single nucleotide<br />
polymorphism, Thailand, adult, genetics, X chromosome linked disorder; Adult, Female, Genetic Diseases, X-Linked, Genome-<br />
Wide Association Study, Humans, Linkage (Genetics), Male, Metalloproteases, Mitochondrial Proteins, Optic Atrophy,<br />
Hereditary, Leber, Polymorphism, Single Nucleotide, Thailand<br />
<strong>Abstract</strong> : Leber hereditary optic neuropathy (LHON) is the most common mitochondrially inherited disease causing blindness,<br />
preferentially in young adult males. Most <strong>of</strong> the patients carry the G11778A mitochondrial DNA (mtDNA) mutation. However,<br />
the marked incomplete penetrance and the gender bias indicate some additional genetic and/or environmental factors to<br />
disease expression. Herein, we first conducted a genome-wide linkage scan with 400 microsatellite markers in 9 large Thai<br />
LHON G11778A pedigrees. Using an affecteds-only nonparametric linkage analysis, 4 regions on chromosomes 3, 12, 13 and 18<br />
showed Zlr scores greater than 2 (P<0.025), which is consistently significant across several linkage statistics. The most<br />
suggestive marker D3S1565 (Zlr>2 in 10 <strong>of</strong> 16 allele sharing models tested) was then expanded to include the region<br />
3q26.2-3q28 covering SLC7A14 (3q26.2), MFN1 (3q26.32), MRPL47 (3q26.33), MCCC1 (3q27.1), PARL (3q27.1) and OPA1 (3q28q29).<br />
All <strong>of</strong> these candidate genes were selected from the Maestro database and had known to be localized in mitochondria.<br />
Sixty tag SNPs were genotyped in 86 cases, 211 <strong>of</strong> their relatives and 32 unrelated Thai controls, by multiplex-PCR-based<br />
Invader assay. Analyses using a powerful association testing tool that adjusts for relatedness (the MQLS statistic) showed the<br />
most evidence <strong>of</strong> association between two SNPs, rs3749446 and rs1402000 (located in PARL presenilins-associated rhomboidlike)<br />
and LHON expression (both P = 8.8×10-5). The mitochondrial PARL protease has been recently known to play a role with<br />
a dynamin-related OPA1 protein in preventing apoptotic events by slowing down the release <strong>of</strong> cytochrome c out <strong>of</strong><br />
mitochondrial cristae junctions. Moreover, PARL is required to activate the intramembranous proteolyses resulting in the<br />
degradation <strong>of</strong> an accumulated proapoptotic protein in the outer mitochondrial membrane. Under these circumstances,<br />
357
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
variants <strong>of</strong> PARL are suggested to influence cell death by apoptosis which has long been believed to intrigue the<br />
neurodegeneration <strong>of</strong> LHON. © <strong>2010</strong> Springer-Verlag.<br />
No.979<br />
Author (s) : Phattanachindakun, B., Boonyagulsrirung, T., Chanprapaph, P.<br />
Title : The correlation in antepartum fetal test between full fetal biophysical pr<strong>of</strong>ile (FBP) and rapid biophysical pr<strong>of</strong>ile (rBPP)<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 7, July <strong>2010</strong>, Pages 759-764<br />
Document Type : Article<br />
Keywords : Amniotic fluid index; Fetal surveillance; Full biophysical pr<strong>of</strong>ile; Rapid biophysical pr<strong>of</strong>ile; Sound-provoked fetal movement<br />
<strong>Abstract</strong> : To determine the correlation between the rapid biophysical pr<strong>of</strong>ile (rBPP), the combination <strong>of</strong> amniotic fluid index (AFI), and<br />
sound-provoked fetal movement (SPFM) detected by ultrasound, and the full biophysical pr<strong>of</strong>ile (FBP) in terms <strong>of</strong> abnormal<br />
and normal result. Material and Method: A prospective study was performed in 200 singleton pregnancies with no fetal<br />
anomalies between 30-42 weeks <strong>of</strong> gestation indicated for non-stress test (NST). All participants received both the standard<br />
(FBP) and the new rBPP examinations. Abnormal fetal test was defined as having a score <strong>of</strong> ≤ 6 for FBP or ≤ 2 for rBPP. The<br />
main outcome measurement was Spearman's correlation coefficient (rs) between both examinations. Results: The incidences<br />
<strong>of</strong> the abnormal tests were 1.5% and 6.0% in FBP and rBPP, respectively. The data showed a positive correlation between the<br />
two tests (rs = 0.67; p < 0.01). Regarding the operative time, FBP assessment was 25.56 ± 8.75 times longer than rBPP. The<br />
number <strong>of</strong> abnormal NST was remarked at 1.5% while oligohydramnios and abnormal SPFM were detected at 5% and 2%,<br />
respectively. Compared to the standard NST, rBPP test was significantly superior in terms <strong>of</strong> correlation with FBP (rs =0.67 vs.<br />
0.33) and shorter duration <strong>of</strong> test (1.21 ± 0.32 min. vs. 21.65 ± 5.47). Conclusion: The statistically significant positive<br />
correlation between rBPP and FBP has been revealed. Due to its simplicity, rapidity, and no need <strong>of</strong> expensive equipment or<br />
experienced interpreter, the rBPP may be alternatively used as a primary antepartum fetal test in the overcrowded obstetric<br />
center or when fetal surveillance tests are limited.<br />
No.980<br />
Author (s) : Phatthiyaphaibun, K., Som-Aum, W., Srisa-Ard, M., Threeprom, J.<br />
Title : Chiral separation <strong>of</strong> pheniramine by capillary electrophoresis partial-filling technique using hydroxypropyl-β-cyclodextrin as<br />
chiral selector<br />
Source : Journal <strong>of</strong> Analytical Chemistry, Volume 65, Issue 8, August <strong>2010</strong>, Pages 803-808<br />
Document Type : Article<br />
Keywords : Applied voltages, Cd concentrations, Chiral selector, Chiral separations, Enantioseparations, Experimental parameters, Eye<br />
drops, Limit <strong>of</strong> detection, Limit <strong>of</strong> quantitations, Pharmaceutical preparations, Phosphate buffers; Capillary<br />
electrophoresis, Chirality, Electrochemistry, Enantiomers; Quality control<br />
<strong>Abstract</strong> : A simple capillary electrophoresis partial-filling technique for the enantioseparation <strong>of</strong> pheniramine is presented. Phosphate<br />
buffer and hydroxypropyl-β-cyclodextrin (HP-β-CD) were used as the electrolyte and chiral selector, respectively. Several<br />
experimental parameters such as HP-β-CD concentration, HP-β-CD plug length, CE temperature and applied voltage were<br />
studied. Under the selected conditions, pheniramine enantiomers can be separated within less than 14 min. The assay was<br />
validated for linearity (5.0 × 10-6-5.0 × 10-4 M; R2 = 0.9987), limit <strong>of</strong> detection (5.0 × 10-7 M), limit <strong>of</strong> quantitation (5.0 × 10-6<br />
M), analytical precision (%RSD ≤ 9.8) and accuracy (%recovery = 101 ± 3). The proposed methodology was then applied to<br />
the analysis <strong>of</strong> a commercially available pharmaceutical eye drop preparation. The results are in good agreement with that<br />
declared by the manufacturer. The proposed methodology provides adequate results in terms <strong>of</strong> simplicity, cost, sample<br />
throughput, repeatability and accuracy for quality control <strong>of</strong> pheniramine enantiomers in pharmaceutical preparations. ©<br />
Pleiades Publishing, Ltd., <strong>2010</strong>.<br />
No.981<br />
Author (s) : Phatthiyaphaibun, K., Som-Aum, W., Srisa-Ard, M., Threeprom, J.<br />
Title : Determination <strong>of</strong> pheniramine enantiomers in eye drop by capillary electrophoresis using hydroxypropyl-β-cyclodextrin as<br />
chiral selector<br />
Source : Journal <strong>of</strong> Analytical Chemistry, Volume 65, Issue 7, July <strong>2010</strong>, Pages 755-759<br />
Document Type : Article<br />
Keywords : Capillary zone electrophoresis, CdS, Chiral selector, Eye drops, Limit <strong>of</strong> detection, Limit <strong>of</strong> quantifications, Phosphate buffers;<br />
Blood vessels, Capillary electrophoresis, Cyclodextrins, Drops, Electrochemistry, Silanes; Data storage equipment<br />
358
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : A capillary zone electrophoresis procedure has been developed for the chiral determination <strong>of</strong> pheniramine in eye drop.<br />
Native and derivative cyclodextrins (CDs) including γ-CD, β-CD, hydroxypropyl-β-CD and dimethyl-β-CD were tested as chiral<br />
selectors. Using 30 mM hydroxypropyl-β-CD in 50 mM phosphate buffer (pH 3.0), the acceptable resolution value (R = 1.55)<br />
was obtained. The assay was validated for linearity (3.3 × 10-6-5.0 × 10-4 M; R 2 = 0.9996), limit <strong>of</strong> detection (3.3 × 10-6 M),<br />
limit <strong>of</strong> quantification (8.5 × 10-6 M), analytical precision by terms <strong>of</strong> intra- and inter-day variability (RSD ≤ 2.57%), and<br />
accuracy (recovery ≥ 89.3%). The content <strong>of</strong> pheniramine in eye drop obtained by the proposed method was in good<br />
agreement with the declared value. The results indicated that pheniramine in the eye drop was present as the racemate. ©<br />
<strong>2010</strong> Pleiades Publishing, Ltd.<br />
No.982<br />
Author (s) : Phawong, C., Ouma, C., Tangteerawatana, P., Thongshoob, J., Were, T., Mahakunkijcharoen, Y., Wattanasirichaigoon, D., Perkins,<br />
D.J., Khusmith, S.<br />
Title : Haplotypes <strong>of</strong> IL12B promoter polymorphisms condition susceptibility to severe malaria and functional changes in cytokine<br />
levels in Thai adults<br />
Source : Immunogenetics, Volume 62, Issue 6, June <strong>2010</strong>, Pages 345-356<br />
Document Type : Article<br />
Keywords : Haplotype; IL12B; P. falciparum; Severe malaria; Single nucleotide polymorphism<br />
<strong>Abstract</strong> : Polymorphic variability in immune response genes, such as IL12B, encoding the IL-12p40 subunit is associated with<br />
susceptibility to severe malaria in African populations. Since the role <strong>of</strong> genetic variation in conditioning severe malaria in Thai<br />
adults is largely unexplored, the functional association between IL12B polymorphisms [i.e. IL12Bpro (rs17860508) and IL12B 3′<br />
UTR T/G (rs3212227)], severe malaria and cytokine production was examined in patients with Plasmodium falciparum<br />
infections (n=355) recruited from malaria endemic areas along the Thai-Myanmar border in northwest Thailand. Circulating IL-<br />
12p40 (p=0.049) and IFN-γ (p=0.051) were elevated in patients with severe malaria, while only IL-12p40 was significantly<br />
higher in severe malaria patients with hyperparasitaemia (p=0.046). Carriage <strong>of</strong> the IL12Bpro1.1 genotype was associated with<br />
enhanced severity <strong>of</strong> malaria (OR, 2.34; 95% CI, 0.94-5.81; p=0.066) and hyperparasitaemia (OR, 3.42; 95% CI, 1.17-9.87;<br />
p=0.025) relative to the IL12Bpro2.2 genotype (wild type). Individuals with the IL12Bpro1.1 genotype also had the lowest IL-<br />
12p40 (p=0.002) and the highest IFN-γ (p=0.004) levels. Construction <strong>of</strong> haplotypes revealed that carriage <strong>of</strong> the IL12Bpro-2/3′<br />
UTR-T haplotype was associated with protection against severe malaria (OR, 0.51; 95% CI, 0.29-0.90; p=0.020) and reduced<br />
circulating IFN-γ (p=0.06). Thus, genotypic and haplotypic variation at IL12Bpro and IL12B 3′ UTR in this population influences<br />
susceptibility to severe malaria and functional changes in circulating IL-12p40 and IFN-γ levels. Results presented here<br />
suggest that protection against severe malaria in Thai adults is associated with genotypic variants that condition enhanced IL-<br />
12p40 and reduced IFN-γ levels. © <strong>2010</strong> Springer-Verlag.<br />
No.983<br />
Author (s) : Phayuhasena, S., Colgan, D.J., Kuvangkadilok, C., Pramual, P., Baimai, V.<br />
Title : Phylogenetic relationships among the black fly species (Diptera: Simuliidae) <strong>of</strong> Thailand based on multiple gene sequences<br />
Source : Genetica, Volume 138, Issue 6, June <strong>2010</strong>, Pages 633-648<br />
Document Type : Article<br />
Keywords : Black fly; Gomphostilbia; Multigene analyses; Nevermannia; Simulium<br />
<strong>Abstract</strong> : Simulium is a very speciose genus <strong>of</strong> the black fly family Simuliidae that includes many important pests <strong>of</strong> humans and<br />
animals. Cytotaxonomic and morphological studies have made substantial progress in Simulium systematics. 16S rRNA and<br />
ITS-1 DNA sequence studies have assisted this progress. Intensive multi-gene molecular systematic investigations will,<br />
however, be required for a comprehensive understanding <strong>of</strong> the genus' taxonomy and evolution. Our research was conducted<br />
to investigate the relationships <strong>of</strong> Thai Simulium at the subgeneric, species group and species levels. We also examined the<br />
possibility <strong>of</strong> using mitochondrial DNA sequences to facilitate Simulium species identification. Data were collected from three<br />
mitochondrial genes (COI, ND4 and 16S rRNA) and two segments <strong>of</strong> the nuclear 28S ribosomal RNA (the D1 to D2 and the D4<br />
expansion regions). The subgenera Simulium and Gomphostilbia were monophyletic in most analyses. Nevermannia included<br />
Montisimulium but was otherwise monophyletic in multigene analyses. In most analyses, Simulium and Nevermannia were<br />
more closely related to each other than to Gomphostilbia which was usually basal. Species groups were generally<br />
monophyletic. Within Gomphostilbia, however, the batoense species group was always paraphyletic to the other two species<br />
groups found in Thailand. Three species groups in Simulium were not monophyletic. The tendency to gill filament number<br />
reduction for some species groups in the subgenus Simulium was associated with a derived position in multigene analyses.<br />
359
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Most species were monophyletic with two exceptions that probably represent species complexes and will present difficulties<br />
for rapid mitochondrial DNA identification. © Springer Science+Business Media B.V. <strong>2010</strong>.<br />
No.984<br />
Author (s) : Phermthai, T., Odglun, Y., Julavijitphong, S., Titapant, V., Chuenwattana, P., Vantanasiri, C., Pattanapanyasat, K.<br />
Title : A novel method to derive amniotic fluid stem cells for therapeutic purposes<br />
Source : BMC Cell Biology, Volume 11, 19 October <strong>2010</strong>, Article number 79<br />
Document Type : Article<br />
Keywords : 5' nucleotidase, beta actin, beta1 integrin, CD133 antigen, endoglin, Hermes antigen, HLA DR antigen, nestin, octamer<br />
transcription factor 4, stage specific embryo antigen 4, Thy 1 antigen, transcription factor NANOG, transcription factor<br />
Sox2, intermediate filament protein, nerve protein, tubulin; adipocyte, amniotic fluid stem cell, article, cell culture, cell<br />
differentiation, cell division, cell isolation, cell lineage, cell population, cell proliferation, colony formation, controlled<br />
study, cytochemistry, human, human cell, intermethod comparison, karyotype, nerve cell, osteocyte, protein<br />
expression, quality control, stem cell, amnion fluid, cell separation, chromosomal<br />
instability, cytology, female, genetics, gestational age, karyotyping, metabolism, methodology, pregnancy; Animalia; Amniotic<br />
Fluid, Cell Separation, Chromosomal Instability, Female, Gestational Age, Humans, Intermediate Filament<br />
Proteins, Karyotyping, Nerve Tissue Proteins, Pregnancy, Stem Cells, Tubulin<br />
<strong>Abstract</strong> : Background: Human amniotic fluid stem (hAFS) cells have become an attractive stem cell source for medical therapy due to<br />
both their ability to propagate as stem cells and the lack <strong>of</strong> ethical debate that comes with the use <strong>of</strong> embryonic stem cells.<br />
Although techniques to derive stem cells from amniotic fluid are available, the techniques have limitations for clinical uses,<br />
including a requirement <strong>of</strong> long periods <strong>of</strong> time for stem cell production, population heterogeneity and xeno-contamination<br />
from using animal antibody-coated magnetic beads. Herein we describe a novel isolation method that fits for hAFS derivation<br />
for cell-based therapy.Methods and Results: With our method, single hAFS cells generate colonies in a primary culture <strong>of</strong><br />
amniotic fluid cells. Individual hAFS colonies are then expanded by subculturing in order to make a clonal hAFS cell line. This<br />
method allows derivation <strong>of</strong> a substantial amount <strong>of</strong> a pure stem cell population within a short period <strong>of</strong> time. Indeed,<br />
108cells from a clonal hAFS line can be derived in two weeks using our method, while previous techniques require two<br />
months. The resultant hAFS cells show a 2-5 times greater proliferative ability than with previous techniques and a<br />
population doubling time <strong>of</strong> 0.8 days. The hAFS cells exhibit typical hAFS cell characteristics including the ability to<br />
differentiate into adipogenic-, osteogenic- and neurogenic lineages, expression <strong>of</strong> specific stem cell markers including Oct4,<br />
SSEA4, CD29, CD44, CD73, CD90, CD105 and CD133, and maintenance <strong>of</strong> a normal karyotype over long culture<br />
periods.Conclusions: We have created a novel hAFS cell derivation method that can produce a vast amount <strong>of</strong> high quality<br />
stem cells within a short period <strong>of</strong> time. Our technique makes possibility for providing autogenic fetal stem cells and<br />
allogeneic cells for future cell-based therapy. © <strong>2010</strong> Phermthai et al; licensee BioMed Central Ltd.<br />
No.985<br />
Author (s) : Phisalprapa, P.; Charatcharoenwitthaya, P.; Chainuvati, S.; Nimanong, S.; Prachayakul, V.; Pausawasdi, N.; Pongprasobchai, S.;<br />
Leelakusolvong, S.; Manatsathit, S.; Kachintorn, U.; Tanwandee, T.<br />
Title : ON-TREATMENT HBSAG LEVEL AND HBEAG INDEX CAN EARLY PREDICT HBEAG SEROCONVERSION IN CHRONIC HEPATITIS B,<br />
HBEAG POSITIVE PATIENTS TREATED WITH PEGINTERFERON ALPHA-2A<br />
Source : JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY, Volume: 25, Supplement: 2, Pages: A94-A94, Published: SEP <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.986<br />
Author (s) : Phokharatkul, P., Nantanitikorn, K., Phaiboon, S.<br />
Title : Thai speech recognition using double filter banks for basic voice commanding<br />
Source : <strong>2010</strong> <strong>International</strong> Conference on Computer, Mechatronics, Control and Electronic Engineering, CMCE <strong>2010</strong>, Volume 6, <strong>2010</strong>,<br />
Article number 5609930, Pages 33-36<br />
Document Type : Conference Paper<br />
Keywords : Double filter banks; Euclidian distance; Thai speech recognition<br />
360
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : This paper describes the methodology to recognize Thai speech words by integrating two approaches e.g., Double filter banks<br />
and Euclidian distance in a feature extraction and the recognition processes, respectively. Firstly, the speech signals are<br />
transformed into the 3-dimension <strong>of</strong> signal or spectrogram. The spectrogram displays energy information along both time and<br />
frequency axes. Secondly, the frequencies to be within the bin spread and correlated them with each triangular filter. Thus,<br />
each bin holds a weighted sum, and represents the spectral magnitude in that filter bank channel. Finally, the filter banks are<br />
normalized into the normalized bank for comparison between the entry signal <strong>of</strong> word and various words <strong>of</strong> dictionary. The<br />
Euclidian distance is used to measure the similarity between them. The system was evaluated for its accuracy and stability in<br />
performing various conditions. The accuracy was tested with 9, 000 speeches from several volunteers. The average accuracy<br />
rate is about 96.3 %. The results show that the evaluation was beyond satisfaction for every aspect. © <strong>2010</strong> IEEE.<br />
No.987<br />
Author (s) : Phomphrai, K., Chumsaeng, P., Sangtrirutnugul, P., Kongsaeree, P., Pohmakotr, M.<br />
Title : Reverse orders <strong>of</strong> reactivities in the polymerization <strong>of</strong> cyclic esters using N2O2 aluminium alkoxide complexes<br />
Source : Dalton Transactions, Volume 39, Issue 7, <strong>2010</strong>, Pages 1865-1871<br />
Document Type : Article<br />
Keywords : Alkoxide complexes, Amine ligand, Caprolactone, Complex 1, Cyclic esters, Electronic effects, H NMR spectroscopy, Lactide<br />
polymerization, Lactides, Polymerization conditions, Polymerization <strong>of</strong> lactide, Polymerization rates, Potassium salts, Relative<br />
reactivities, Reverse order, Ring-opened products, Side chains, Steric hindrances; Aluminum, Dyes, Esters, Nuclear magnetic<br />
resonance spectroscopy, Polymerization, Synthesis (chemical), Toluene, X ray crystallography; Ligands;<br />
aluminum, ester, nitrogen oxide, organometallic compound; article, chemical<br />
structure, chemistry, cyclization, stereoisomerism, synthesis, X ray crystallography; Aluminum, Crystallography, X-<br />
Ray, Cyclization, Esters, Models, Molecular, Molecular Structure, Nitrogen Oxides, Organometallic<br />
Compounds, Stereoisomerism<br />
<strong>Abstract</strong> : Three aluminium alkoxide complexes containing N2O2 bis(phenoxy)-amine ligands were synthesized from reactions <strong>of</strong> the<br />
corresponding N2O2 ligands with Al(OiPr)3 in toluene. Different amine side chains <strong>of</strong> the ligands included pyridine (1),<br />
CH2NMe2 (2), and CH2NEt2 (3). The related chloro aluminium analog (4) was prepared from a reaction between AlCl3 and the<br />
potassium salt <strong>of</strong> the N2O2 ligand having CH2NMe2 side chain. X-Ray crystallography reveals that complexes 3 and 4 have a<br />
monomeric five-coordinate aluminium center. Complexes 1-3 catalyzed the polymerization <strong>of</strong> ε-caprolactone (ε-CL) at 70 °C<br />
in toluene with the relative reactivities <strong>of</strong> 1 < 2 < 3. In contrary, only complex 1 was active for the polymerization <strong>of</strong><br />
lactide under the same polymerization conditions. 1H NMR spectroscopy shows that treatment <strong>of</strong> 2 with 1 equivalent <strong>of</strong><br />
lactide afforded the ring-opened product L2Al-OCH(Me)C(O)OCH(Me)C(O)OiPr. Electronic effects are believed to be<br />
responsible for the observed trend in the ε-CL polymerization rates. On the other hand, steric hindrance at the amine side<br />
chain is the main contributor to the observed rates <strong>of</strong> lactide polymerization. © The Royal Society <strong>of</strong> Chemistry <strong>2010</strong>.<br />
No.988<br />
Author (s) : Phornphisutthimas, S., Sudtachat, N., Bunyoo, C., Chotewutmontri, P., Panijpan, B., Thamchaipenet, A.<br />
Title : Development <strong>of</strong> an intergeneric conjugal transfer system for rimocidin-producing Streptomyces rimosus<br />
Source : Letters in Applied Microbiology, Volume 50, Issue 5, May <strong>2010</strong>, Pages 530-536<br />
Document Type : Article<br />
Keywords : Actinomycetes; Antibiotics; Molecular genetics; Plasmids; Streptomycetes<br />
<strong>Abstract</strong> : Aims: To develop an intergeneric conjugation system for rimocidin-producing Streptomyces rimosus. Methods and Results:<br />
High efficiencies <strong>of</strong> conjugation [10-2-10-3 transconjugants/recipient colony forming units (CFU)] were obtained when spores <strong>of</strong><br />
S. rimosus were heat treated at 40°C for 10 min prior to mixing with E. coli ET12567(pUZ8002/pIJ8600) as donor. Mycelium<br />
from liquid grown cultures <strong>of</strong> S. rimosus could also be used as recipient instead <strong>of</strong> spores, with 24-h cultures giving optimal<br />
results. TSA (Oxoid) medium containing 10 m mol l-1 MgCl2 was the preferred medium for conjugation. Southern<br />
hybridization was used to confirm that transconjugants <strong>of</strong> S. rimosus contained a single copy <strong>of</strong> pIJ8600 integrated at a unique<br />
chromosomal attachment site (attB). The transconjugants exhibited a high stability <strong>of</strong> plasmid integration and showed strong<br />
expression <strong>of</strong> green fluorescent protein when using pIJ8655 as the conjugative vector. Conclusion: Intergeneric conjugation<br />
between E. coli and S. rimosus was achieved at high efficiency using both spores and mycelium. Significance and Impact <strong>of</strong><br />
the Study: The conjugation system developed provides a convenient gene expression system for S. rimosus R7 and will<br />
enable the genetic manipulation <strong>of</strong> the rimocidin gene cluster. © <strong>2010</strong> The Authors. Journal compilation © <strong>2010</strong> The Society<br />
for Applied Microbiology.<br />
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No.989<br />
Author (s) : Phosrithong, N., Ungwitayatorn, J.<br />
Title : Molecular docking study on anticancer activity <strong>of</strong> plant-derived natural products<br />
Source : Medicinal Chemistry Research, Volume 19, Issue 8, November <strong>2010</strong>, Pages 817-835<br />
Document Type : Article<br />
Keywords : Anticancer; Docking; Natural products<br />
<strong>Abstract</strong> : A variety <strong>of</strong> compounds from plant sources have been reported to possess substantial anticancer properties; however, their<br />
modes <strong>of</strong> action have not been clearly defined. Selected plant-derived compounds that exhibit anticancer activity were<br />
subjected to docking simulations using AutoDock 3.0.5. To preliminarily investigate the potential molecular targets and to<br />
confirm the experimental activity testing for these anticancer compounds, the docking was performed using different<br />
enzymes and receptor proteins involved with cell cycle, cell growth, and DNA replication, i.e., cyclin-dependent protein<br />
kinase 2 (CDK-2), CDK-6, DNA topoisomerases I and II, B-cell lymphoma 2 (Bcl-2), vascular endothelial growth factor receptor 2<br />
(VEGFR-2), and the telomere: G-quadruplexes. The docking results revealed that lupeol exhibited better binding interaction to<br />
CDK-2 and Bcl-2 than the known CDK-2 and Bcl-2 inhibitors. Epigallocatechin gallate (EGCG) was found to bind to CDK-6 with<br />
tighter interaction than several reported CDK-6 inhibitors. Flavopiridol, a synthetic flavonoid, was best bound to DNA<br />
topoisomerase I. Green tea catechin was best docked with topoisomerase II and VEGFR-2 and quercetin showed very good<br />
binding interaction with telomere: G-quadruplex. The experimental-derived inhibition constant (Ki) against Bcl-2 and Ki<br />
calculated from docking energy were well correlated. Therefore, the calculated Ki could be used as a preliminary tool for<br />
screening <strong>of</strong> Bcl-2 inhibitors before performing experimental activity assay. © Birkhaüser Boston 2009.<br />
No.990<br />
Author (s) : Phusaanantakul, P., Promsonthi, P., Chanrachakul, B.<br />
Title : Effect <strong>of</strong> isosorbide mononitrate for cervical ripening before surgical termination <strong>of</strong> pregnancy in the first trimester<br />
Source : <strong>International</strong> Journal <strong>of</strong> Gynecology and Obstetrics, Volume 110, Issue 2, August <strong>2010</strong>, Pages 145-148<br />
Document Type : Article<br />
Keywords : Cervical ripening; First trimester termination; Isosorbide mononitrate; Nitric oxide; Surgical abortion<br />
<strong>Abstract</strong> : Objective: To evaluate the efficacy <strong>of</strong> isosorbide mononitrate (IMN) for cervical ripening prior to first trimester surgical<br />
termination <strong>of</strong> pregnancy. Methods: A prospective, double-blind, randomized, placebo-controlled trial. Women scheduled for<br />
surgical termination <strong>of</strong> a nonviable fetus before 12 weeks <strong>of</strong> gestation from October 2008 to June 2009 were enrolled and<br />
randomly assigned to receive either 20 mg vaginally <strong>of</strong> IMN (n = 24) or a placebo (n = 24) 4 hours before suction evacuation.<br />
Cervical dilation before evacuation was assessed with 10-mm Hegar dilators followed by smaller sizes that were measured<br />
until the instrument passed freely through the internal os. Cervical dilation, adverse effects, termination complications, and<br />
patient satisfaction were the main outcomes. Results: Mean cervical dilation was not significantly different between the IMN<br />
and placebo groups (6.29 ± 0.99 mm vs 5.71 ± 1.04 mm; P = 0.05). Mean operative time did not differ between the groups<br />
(16 ± 0.07 min vs 18 ± 0.06 min; P = 0.55), nor did patient satisfaction measured by visual analogue scale (7.04 ± 1.68 vs 6.54<br />
± 1.22; P = 0.24). Conclusion: IMN was comparable to placebo in terms <strong>of</strong> efficacy and patient satisfaction for cervical priming<br />
prior to first-trimester termination <strong>of</strong> pregnancy. © <strong>2010</strong> Published by Elsevier Ireland Ltd.<br />
No.991<br />
Author (s) : Phuwapraisirisan, S., Lim, M., Suwanthanma, W.<br />
Title : Surgical reduction in a delayed case <strong>of</strong> traumatic testicular dislocation<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 11, November <strong>2010</strong>, Pages 1317-1320<br />
Document Type : Article<br />
Keywords : Dislocated testis; Surgical reduction; Traumatic testicular dislocation<br />
<strong>Abstract</strong> : A 27-year-old Thai male was admitted with left groin pain and he felt that he lost his left testis three weeks after his<br />
motorcycle accident-car collision. He had not been diagnosed after four days <strong>of</strong> admission at the previous hospital. Surgical<br />
reduction was performed without acute and delayed complications after three months <strong>of</strong> follow-up. Traumatic testicular<br />
dislocation is an uncommon sequel <strong>of</strong> trauma usually related to straddle injury from motorcycle accidents. Diagnosis<br />
depends on the awareness <strong>of</strong> the physician <strong>of</strong> its possibility <strong>of</strong> occurrence. Physical examination is the most helpful in<br />
diagnosis. CT scan or Doppler ultrasound, if available, may be helpful in diagnosis, particularly locating the extrascrotal<br />
testicle and detecting testicular viability. Manual reduction is the treatment <strong>of</strong> choice for acute traumatic dislocation <strong>of</strong> testis.<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Open reduction is indicated for the delayed case, or if there are 1) difficulty in determining the integrity <strong>of</strong> dislocated testis, 2)<br />
possibility <strong>of</strong> torsion, 3) failure <strong>of</strong> close reduction, or 4) the minimal morbidity <strong>of</strong> an inguinal exploration.<br />
No.992<br />
Author (s) : Piaseu, N., Komindr, S., Belza, B.<br />
Title : Understanding food insecurity among Thai older women in an urban community<br />
Source : Health Care for Women <strong>International</strong>, Volume 31, Issue 12, December <strong>2010</strong>, Pages 1110-1127<br />
Document Type : Article<br />
Keywords : aged, article, Asian, attitude to health, catering service, demography, economics, ethnology, family<br />
size, female, food, human, interview, middle aged, psychological aspect, qualitative<br />
research, questionnaire, socioeconomics, Thailand, urban population; Aged, Asian Continental Ancestry Group, Family<br />
Characteristics, Female, Food, Food Supply, Health Knowledge, Attitudes, Practice, Humans, Interviews as Topic, Middle<br />
Aged, Qualitative Research, Questionnaires, Residence Characteristics, Socioeconomic Factors, Thailand, Urban<br />
Population, Women<br />
<strong>Abstract</strong> : In this qualitative study we aimed to describe how older women in a crowded urban community perceive the food insecurity<br />
experience and deal with it. In-depth interviews were conducted among 30 Thai older women. Results revealed that older<br />
women perceived their food insecurity experience as a negative effect <strong>of</strong> the current economic downturn globally. They felt<br />
that they were confronting a crisis. Problems they dealt with included six issues. The women employed management<br />
strategies around food, health, money, and family. Our results suggest a need for welfare reform that facilitates management<br />
strategies aimed to meet food security. © Taylor & Francis Group, LLC.<br />
No.993<br />
Author (s) : Piboonpocanun, S., Boonchoo, S., Pariyaprasert, W., Visitsunthorn, N., Jirapongsananuruk, O.<br />
Title : Determination <strong>of</strong> storage conditions for shrimp extracts: Analysis <strong>of</strong> specific IgE-Allergen pr<strong>of</strong>iles<br />
Source : Asian Pacific Journal <strong>of</strong> Allergy and Immunology, Volume 28, Issue 1, March <strong>2010</strong>, Pages 47-52<br />
Document Type : Article<br />
Keywords : allergen, immunoglobulin E, shrimp extract, unclassified drug; adolescent, allergenicity, article, child, clinical article, food<br />
allergy, food storage, human, immunoblotting, informed consent, patient selection, polyacrylamide gel<br />
electrophoresis, preschool child, prick test, school child, serology, shrimp, skin test, storage, storage temperature;<br />
Adolescent, Allergens, Animals, Blotting, Western, Cell Extracts, Child, Child, Preschool, Cryopreservation, Food<br />
Hypersensitivity, Humans, Immunoglobulin E, Penaeidae, Reagent Kits, Diagnostic, Shellfish<br />
<strong>Abstract</strong> : The consumption <strong>of</strong> shrimp is a common cause <strong>of</strong> food hypersensitivity reactions. Shrimp allergy is diagnosed using a skin<br />
prick test (SPT) as well as by food challenges. Due to the lack <strong>of</strong> a wide variety <strong>of</strong> commercial shrimp extracts for SPTs, we<br />
selected various shrimp species for the preparation <strong>of</strong> local shrimp extracts. However, optimal storage conditions for the<br />
shrimp extracts which also maintains allergenic potency has not yet been identified. The objective <strong>of</strong> the present study was<br />
to determine the potency <strong>of</strong> the shrimp extracts under different storage conditions and durations. Specific IgE-Allergen<br />
pr<strong>of</strong>iles <strong>of</strong> eight shrimp-allergic patients were investigated by using sera incubated with extracts prepared from lyophilized raw<br />
or boiled shrimp, which were stored at 4°C or -20°C for up to 4 weeks. When stored at -20°C, most allergens were preserved<br />
after 4 weeks. However, storage at 4°C results in few allergens remaining after 2 weeks. Boiled-shrimp extracts stored at 4°C<br />
and -20°C contained higher amounts <strong>of</strong> IgE-allergen complexes than raw-shrimp extracts. Moreover, in both raw and boiled<br />
shrimp extracts, the IgE bound 36-40 kDa allergens constituted the major proteins since they were observed in all IgE-allergen<br />
pr<strong>of</strong>iles. In conclusion, we recommend that shrimp extracts are stored at -20°C for 4 weeks to prevent the loss <strong>of</strong> allergens.<br />
No.994<br />
Author (s) : Pichainarong, N.; Chaveepojnkamjorn, W.<br />
Title : YOUTH AND ALCOHOLIC BEVERAGES DRINKING PATTERNS AMONG HIGH SCHOOL STUDENTS IN CENTRAL THAILAND<br />
Source : SOUTHEAST ASIAN JOURNAL OF TROPICAL MEDICINE AND PUBLIC HEALTH, Volume: 41, Issue: 6, Pages: 1467-1474, Published:<br />
NOV <strong>2010</strong><br />
Document Type : Article<br />
Keywords : drinking patterns; high school students; central Thailand<br />
<strong>Abstract</strong> : The objective <strong>of</strong> this study was to determine the drinking patterns <strong>of</strong> high school students in central Thailand Eleven<br />
thousand three hundred sixty high school students from central Thailand were divided into 2 groups (drinkers and<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
nondrinkers) according to their alcohol consumption Information was obtained by an anonymous self-reporting questionnaire<br />
which consisted <strong>of</strong> 2 parts general characteristics, and characteristics <strong>of</strong> alcohol drinking behavior Data were analyzed using<br />
descriptive and inferential statistics by a computerized statistical package The socio-demographic factors related to the<br />
student's alcohol consumption during the previous 12 months were age > 15 years old, male sex, grades 9 and 11 education<br />
level, living in a private dormitory, staying with a relative or a friend, having a grade point average 30, having a job<br />
earning money and having family members with alcohol/drug problems (pCAA, α2) and Paksé (codon 142, TAA>TAT, α2) mutations in<br />
Thailand<br />
Source : Hemoglobin, Volume 34, Issue 6, December <strong>2010</strong>, Pages 582-586<br />
Document Type : Article<br />
Keywords : Dot-blot hybridization; Elongated α-globin chain; Gene frequency; Hb Constant Spring (Hb CS); Hb Paksé<br />
<strong>Abstract</strong> : Hb Constant Spring [Hb CS, α142(H19)Term] and Hb Paksé [α142(H19)Term] occur from the mutation in the termination<br />
codon <strong>of</strong> the α2-globin gene, TAA>CAA (→Gln) and TAA>TAT (→Tyr), respectively. They are the most common<br />
nondeletional α-thalassemia (α-thal) variants causing Hb H disease in Southeast Asia. In this study, 587 cord blood samples<br />
were screened for the Hb CS and Hb Paksé mutations by a dot-blot hybridization technique using oligonucleotide probes<br />
specific for each mutation. The results showed that the prevalence <strong>of</strong> Hb CS and Hb Paksé in Central Thailand are 5.80 and<br />
0.51%, respectively, which is in concordance with the results from previous studies. © <strong>2010</strong> Informa Healthcare USA, Inc.<br />
No.996<br />
Author (s) : Pillas, D., Hoggart, C.J., Evans, D.M., O'Reilly, P.F., Sipilä, K., Lähdesmäki, R., Millwood, I.Y., Kaakinen, M., Netuveli, G., Blane, D.,<br />
Charoen, P., Sovio, U., Pouta, A., Freimer, N., Hartikainen, A.-L., Laitinen, J., Vaara, S., Glaser, B., Crawford, P., Timpson, N.J.,<br />
Ring, S.M., Deng, G., Zhang, W., McCarthy, M.I., Deloukas, P., Peltonen, L., Elliott, P., Coin, L.J.M., Smith, G.D., Jarvelin, M.-R.<br />
Title : Genome-wide association study reveals multiple loci associated with primary tooth development during infancy<br />
Source : PLoS Genetics, Volume 6, Issue 2, February <strong>2010</strong>, Article number e1000856<br />
Document Type : Article<br />
Keywords : ectodysplasin A, high mobility group A2 protein, Hox protein, insulin like growth factor 2 binding protein 1, inwardly rectifying<br />
potassium channel subunit Kir2.1, methionine sulfoxide reductase B, methionine sulfoxide reductase B3, nucleic acid binding<br />
protein, protein HoxB2, protein rad51l1, somatomedin binding protein, unclassified drug; article, chromosomal<br />
localization, gene cluster, gene frequency, gene linkage disequilibrium, gene locus, human, infancy, organogenesis, single<br />
nucleotide polymorphism, tooth development, tooth eruption, allele, birth, deciduous tooth, female, Finland, genetic<br />
association, genetics, genotype, growth, development and aging, infant, longitudinal study, male, meta analysis, United<br />
Kingdom; Alleles, England, Female, Finland, Genetic Loci, Genome-Wide Association Study, Genotype, Humans, Infant, Linkage<br />
Disequilibrium, Longitudinal Studies, Male, Meta-Analysis as Topic, Parturition, Polymorphism, Single Nucleotide, Tooth<br />
Eruption, Tooth, Deciduous<br />
<strong>Abstract</strong> : Tooth development is a highly heritable process which relates to other growth and developmental processes, and which<br />
interacts with the development <strong>of</strong> the entire crani<strong>of</strong>acial complex. Abnormalities <strong>of</strong> tooth development are common, with<br />
tooth agenesis being the most common developmental anomaly in humans. We performed a genome-wide association study<br />
<strong>of</strong> time to first tooth eruption and number <strong>of</strong> teeth at one year in 4,564 individuals from the 1966 Northern Finland Birth<br />
Cohort (NFBC1966) and 1,518 individuals from the Avon Longitudinal Study <strong>of</strong> Parents and Children (ALSPAC). We identified 5<br />
loci at P<5×10 -8, and 5 with suggestive association (P<5×10-6). The loci included several genes with links to tooth and<br />
other organ development (KCNJ2, EDA, HOXB2, RAD51L1, IGF2BP1, HMGA2, MSRB3). Genes at four <strong>of</strong> the identified loci are<br />
364
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
implicated in the development <strong>of</strong> cancer. A variant within the HOXB gene cluster associated with occlusion defects requiring<br />
orthodontic treatment by age 31 years. © <strong>2010</strong> Pillas et al.<br />
No.997<br />
Author (s) : Pimpha, N., Sunintaboon, P., Inphonlek, S., Tabata, Y.<br />
Title : Gene delivery efficacy <strong>of</strong> polyethyleneimine-introduced chitosan shell/poly(methyl methacrylate) core nanoparticles for rat<br />
mesenchymal stem cells<br />
Source : Journal <strong>of</strong> Biomaterials Science, Polymer Edition, Volume 21, Issue 2, 1 January <strong>2010</strong>, Pages 205-223<br />
Document Type : Article<br />
Keywords : Chitosan; Mesenchymal stem cells; Nanoparticles; Non-viral carrier; Polyethyleneimine<br />
<strong>Abstract</strong> : This work investigated polyethyleneimine (PEI)-introduced chitosan (CS) (CS/PEI) nanoparticles as non-viral carrier <strong>of</strong> plasmid<br />
DNA for rat mesenchymal stem cells (MSCs). The CS/PEI nanoparticles were prepared by the emulsifier-free emulsion<br />
polymerization <strong>of</strong> methyl methacrylate monomer induced by a small amount <strong>of</strong> t-butyl hydroperxide in the presence <strong>of</strong><br />
different concentrations <strong>of</strong> PEI mixed with CS. The resulting nanoparticles were characterized by their surface properties and<br />
buffering capacity. In vitro gene transfection was also evaluated. The introduction <strong>of</strong> PEI affected the surface charge,<br />
dispersing stability and buffering capacity <strong>of</strong> the nanoparticles. The CS/PEI nanoparticles formed a complex upon mixing with<br />
a plasmid DNA <strong>of</strong> luciferase. The complex enhanced the level <strong>of</strong> gene transfection and prolonged the time period <strong>of</strong><br />
expression for MSCs, compared with those <strong>of</strong> plasmid DNA-original CS and PEI nanoparticles. Cytotoxicity <strong>of</strong> CS/PEI complexes<br />
with plasmid DNA was significantly low, depending on the amount <strong>of</strong> PEI introduced. It is concluded that the CS/PEI<br />
nanoparticle was a promising carrier for gene delivery <strong>of</strong> MSCs. © <strong>2010</strong> Koninklijke Brill NV, Leiden.<br />
No.998<br />
Author (s) : Pinchai, N., Juvvadi, P.R., Fortwendel, J.R., Zachary Perfect, B., Rogg, L.E., Asfaw, Y.G., Steinbach, W.J.<br />
Title : The aspergillus fumigatus p-type golgi apparatus Ca2+/Mn2+ ATPase pmra is involved in cation homeostasis and cell wall<br />
integrity but is not essential for pathogenesis<br />
Source : Eukaryotic Cell, Volume 9, Issue 3, March <strong>2010</strong>, Pages 472-476<br />
Document Type : Note<br />
Keywords : adenosine triphosphatase (calcium), aminoglycoside, antifungal agent, beta<br />
glucan, casp<strong>of</strong>ungin, cation, chitin, echinocandin, egtazic acid, fungal protein, nikkomycin, sodium chloride, sorbitol;<br />
animal, antifungal resistance, Aspergillus fumigatus, cell proliferation, cell wall, drug effect, enzymology, gene<br />
expression, genetics, Golgi complex, homeostasis, Kaplan Meier method, lung, lung<br />
aspergillosis, male, metabolism, mouse, mouse strain, note, pathogenicity, pathology, pH, physiological stress, upregulation;<br />
Aminoglycosides, Animals, Antifungal Agents, Aspergillus fumigatus, beta-Glucans, Calcium-Transporting ATPases, Cations, Cell<br />
Proliferation, Cell Wall, Chitin, Drug Resistance, Fungal, Echinocandins, Egtazic Acid, Fungal Proteins, Gene Expression, Golgi<br />
Apparatus, Homeostasis, Hydrogen-Ion Concentration, Invasive Pulmonary Aspergillosis, Kaplan-Meiers<br />
Estimate, Lung, Male, Mice, Mice, Inbred Strains, Saline Solution, Hypertonic, Sorbitol, Stress, Physiological, Up-Regulation;<br />
Aspergillus fumigatus<br />
<strong>Abstract</strong> : The Aspergillus fumigatus δpmrA (Golgi apparatus Ca2+/Mn2+ P-type ATPase) strain has osmotically suppressible basal<br />
growth defects and cationic tolerance associated with increased expression <strong>of</strong> calcineurin pathway genes. Despite increased<br />
β-glucan and chitin content, it is hypersensitive to cell wall inhibitors but remains virulent, suggesting a role for PmrA in<br />
cation homeostasis and cell wall integrity. © <strong>2010</strong>, American Society for Microbiology. All Rights Reserved.<br />
No.999<br />
Author (s) : Pinpathomrat, N.; Kraweefrengfu, T.; Laphodom, A.; Islam, N.E.; Kirawanich, P.<br />
Title : Mosquito larva inviability study through pulsed electric field exposures<br />
Source : Plasma Science, <strong>2010</strong> <strong>Abstract</strong>s IEEE <strong>International</strong> Conference on Issue Date: 20-24 June <strong>2010</strong> On page(s): 1 - 1<br />
Document Type : Conference Paper<br />
Keywords : –<br />
<strong>Abstract</strong> : There are many methods used for mosquito control, for example, habitat modification, such as removing stagnant water from<br />
potential breeding areas, chemical pesticide, natural predators, such as dragonflies and larvae-eating fish, and trapping. The<br />
principal objective <strong>of</strong> this work is to study the effects <strong>of</strong> pulsed electric fields (PEFs) on mosquito larva inviability. Fourth<br />
instar larvae <strong>of</strong> Culex quinquefasciatus were hand-graded from the same stagnant water supply and divided into 24 groups <strong>of</strong><br />
365
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
20 larvae for inviability testing. There were a total <strong>of</strong> 4 experimental sets for 4 different combinations <strong>of</strong> exposure parameters.<br />
Each set consists <strong>of</strong> three groups <strong>of</strong> treatment replications and three groups <strong>of</strong> non-treated larvae as associated controls.<br />
Each group was placed in transparent plastic cup and firmly situated inside the laboratory-constructed treatment chamber<br />
except the control. The pulse forming line (PFL) was used as a pulse generator. The cups were incubated for 5 days, i.e. close<br />
to normal hatching time for mosquito larvae, at room temperature <strong>of</strong> approximately 28°C for normal morphogenesis to take<br />
place. The inviability was determined at 24, 48, 72, 96 and 120 hours. The mosquito larvae were considered inviable if<br />
deceased. The effects <strong>of</strong> PEFs can be observed when the exposure parameters were 5 kV-15 min and 15 kV-5 min.<br />
No.1000<br />
Author (s) : Piola, P., Nabasumba, C., Turyakira, E., Dhorda, M., Lindegardh, N., Nyehangane, D., Snounou, G., Ashley, E.A., McGready, R.,<br />
Nosten, F., Guerin, P.J.<br />
Title : Efficacy and safety <strong>of</strong> artemether-lumefantrine compared with quinine in pregnant women with uncomplicated Plasmodium<br />
falciparum malaria: an open-label, randomised, non-inferiority trial<br />
Source : The Lancet Infectious Diseases, Volume 10, Issue 11, November <strong>2010</strong>, Pages 762-769<br />
Document Type : Article<br />
Keywords : artemether plus benflumetol, quinine; abdominal pain, adolescent, adult, anorexia, article, clinical trial, controlled clinical<br />
trial, controlled study, dizziness, drug efficacy, drug induced headache, drug safety, eosinophil, female, flu like syndrome,<br />
follow up, heart disease, human, lymphocytopenia, major clinical study, malaria falciparum, maternal<br />
mortality, nausea, newborn death, polydactyly, polymerase chain reaction, pregnant woman, prenatal care, priority journal,<br />
QT prolongation, randomization, randomized controlled trial, second trimester pregnancy, side effect, spontaneous abortion,<br />
third trimester pregnancy, thrombocyte, tinnitus, treatment outcome, vomiting, weakness; Adolescent, Adult, Animals,<br />
Antimalarials, Artemisinins, Drug Combinations, Ethanolamines, Female, Fluorenes, Humans, Malaria, Falciparum, Plasmodium<br />
falciparum, Polymerase Chain Reaction, Pregnancy, Pregnancy Complications, Infectious, Quinine, Treatment Outcome,<br />
Uganda, Young Adult<br />
<strong>Abstract</strong> : Background: Malaria in pregnancy is associated with maternal and fetal morbidity and mortality. In 2006, WHO recommended<br />
use <strong>of</strong> artemisinin-based combination treatments during the second or third trimesters, but data on efficacy and safety in<br />
Africa were scarce. We aimed to assess whether artemether-lumefantrine was at least as efficacious as oral quinine for the<br />
treatment <strong>of</strong> uncomplicated falciparum malaria during the second and third trimesters <strong>of</strong> pregnancy in Mbarara, Uganda.<br />
Methods: We did an open-label, randomised, non-inferiority trial between October, 2006, and May, 2009, at the antenatal<br />
clinics <strong>of</strong> the Mbarara <strong>University</strong> <strong>of</strong> Science and Technology Hospital in Uganda. Pregnant women were randomly assigned<br />
(1:1) by computer generated sequence to receive either quinine hydrochloride or artemether-lumefantrine, and were<br />
followed up weekly until delivery. Our primary endpoint was cure rate at day 42, confirmed by PCR. The non-inferiority<br />
margin was a difference in cure rate <strong>of</strong> 5%. Analysis <strong>of</strong> efficacy was for all randomised patients without study deviations that<br />
could have affected the efficacy outcome. This study was registered with ClinicalTrials.gov, number NCT00495508. Findings:<br />
304 women were randomly assigned, 152 to each treatment group. By day 42, 16 patients were lost to follow-up and 25<br />
were excluded from the analysis. At day 42, 137 (99·3%) <strong>of</strong> 138 patients taking artemether-lumefantrine and 122 (97·6%) <strong>of</strong><br />
125 taking quinine were cured-difference 1·7% (lower limit <strong>of</strong> 95% CI -0·9). There were 290 adverse events in the quinine<br />
group and 141 in the artemether-lumefantrine group. Interpretation: Artemisinin derivatives are not inferior to oral quinine for<br />
the treatment <strong>of</strong> uncomplicated malaria in pregnancy and might be preferable on the basis <strong>of</strong> safety and efficacy. Funding:<br />
Médecins Sans Frontières and the European Commission. © <strong>2010</strong> Elsevier Ltd.<br />
No.1001<br />
Author (s) : Piriyapatsom, A., Pranootnarabha, T., Uerpairojkit, K., Punjasawadwong, Y., Chumnanvej, D., Tanudsintum, S.<br />
Title : Difficult intubation in the adult patients undergoing oropharygolaryngeal, neck, and maxill<strong>of</strong>acial procedures: Thai anesthesia<br />
incident monitoring study (Thai AIMS)<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 12, December <strong>2010</strong>, Pages 1391-1398<br />
Document Type : Article<br />
Keywords : Difficult intubation; Maxill<strong>of</strong>acial procedure; Neck; Oropharyngeal; Thai AIMS<br />
<strong>Abstract</strong> : Background: Difficult intubation is one <strong>of</strong> the common anesthetic related complications during the perioperative period. In<br />
the patients with pathology or disease involving the oropharyngolarynx, neck, or maxillo-facial region, they might have a<br />
potentially higher risk than the general population. Objective: To determine the characteristics and the contributing factors <strong>of</strong><br />
difficult intubation in the adult patients undergoing oropharyngolaryngeal, neck, and maxill<strong>of</strong>acial procedures, and the factors<br />
366
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
minimizing this incidence and the suggested corrective strategies. Material and Method: All incident reports <strong>of</strong> difficult<br />
intubation in the adult patients who received general anesthesia for the procedure involving the oropharyngolarynx, neck,<br />
and maxill<strong>of</strong>acial region from the Thai Anesthesia Incident Monitoring Study (Thai AIMS) database were identified. The details<br />
<strong>of</strong> the reports, the contributing factors, the factors those minimizing the incident, and the suggestive corrective strategies were<br />
studied. Results: There were 26 (1.3%) incident reports <strong>of</strong> difficult intubation from the database. This occurred in 35%, 58%,<br />
and 15% <strong>of</strong> the patients with Mallampati class 1-2, thyromental distance equal or more than 5 cm or 3 fingerbreadths, and<br />
combined both parameters, respectively. Forty two percent <strong>of</strong> cases were judged as an unplanned difficult intubation.<br />
Twentyseven, 23, and 19 percent <strong>of</strong> the patients had tumor or carcinoma at the oropharyngolarynx, deep neck infection, and<br />
maxill<strong>of</strong>acial fracture, respectively. Nearly half <strong>of</strong> the adverse events accompanied with difficult intubation were desaturation.<br />
No immediate fatally and late outcome was reported. Patients' disease/anatomy was the major contributing factor that might<br />
relate to the incidence. Previous experience, experienced assistance, and high vigilance were the factors minimizing incidence.<br />
Suggestive corrective strategies were guideline practices, additional training, and improved supervision. Conclusion: Carefully<br />
preoperative airway assessment and additional attention focused on the pathology or disease were the principle tasks.<br />
Algorithms for both anticipated and unanticipated difficult airway as well as alternative airway equipments should be<br />
implemented.<br />
No.1002<br />
Author (s) : Pirojsakul, K.; Tangnararatchakit, K.; Kositwattanarerk, A.; Chouplywech, P.; Anuratthapan, U.; Tapaneya-Olarn, W.<br />
Title : Assessment <strong>of</strong> Renal Function by Cystatin C Level in Pediatric Patients before and after Induction Phase <strong>of</strong> Chemotherapy<br />
Source : PEDIATRIC NEPHROLOGY, Volume: 25, Issue: 9, Pages: 1859-1859, Meeting <strong>Abstract</strong>: 323, Published: SEP <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1003<br />
Author (s) : Pirompul, N.; Govitrapong, P.; Chetsawang, B.<br />
Title : FARNESYLTRANSFERASE INHIBITOR ATTENUATES METHAMPHETAMINE-INDUCED CELL DEATH IN NEUROBLASTOMA SH-SY5Y<br />
CULTURED CELLS<br />
Source : JOURNAL OF NEUROCHEMISTRY, Volume: 115, Supplement: 1, Pages: 80-81, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1004<br />
Author (s) : Piroonratana, T., Wongseree, W., Usavanarong, T., Assawamakin, A., Limwongse, C., Chaiyaratana, N.<br />
Title : Identification <strong>of</strong> ancestry informative markers from chromosome-wide single nucleotide polymorphisms using symmetrical<br />
uncertainty ranking<br />
Source : Proceedings - <strong>International</strong> Conference on Pattern Recognition, <strong>2010</strong>, Article number 5595752, Pages 2448-2451<br />
Document Type : Conference Paper<br />
Keywords : Ancestry informative marker; Attribute selection; HapMap; Pattern recognition; Single nucleotide polymorphism<br />
<strong>Abstract</strong> : Ancestry informative markers (AIMs) have been proven to contain necessary information for population classification. In this<br />
article, round robin symmetrical uncertainty ranking for preliminary AIM screening is proposed. Each single nucleotide<br />
polymorphism (SNP) is assigned a rank based on its ability to separate two populations from each other. In a multi-population<br />
scenario, all possible population pairs are considered and the screened SNP set incorporates top-ranked SNPs from every<br />
pair-wise comparison. After the preliminary screening, SNPs are further screened by a wrapper which is embedded with a<br />
naive Bayes classifier. A classification model is subsequently constructed from the finally screened SNPs via a naive Bayes<br />
classifier. The application <strong>of</strong> the proposed procedure to the HapMap data indicates that AIM panels can be found on all<br />
chromosomes. Each panel consists <strong>of</strong> 11 to 24 SNPs and can be used to completely classify the CEU, CHB, JPT and YRI<br />
populations. Moreover, all panels are smaller than the AIM panels reported in previous studies. © <strong>2010</strong> IEEE.<br />
No.1005<br />
Author (s) : Pisetkul, C., Chanchairujira, K., Chotipanvittayakul, N., Ong-Ajyooth, L., Chanchairujira, T.<br />
367
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Malnutrition-inflammation score associated with atherosclerosis, inflammation and short-term outcome in hemodialysis<br />
patients.<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand = Chotmaihet thangphaet, Volume 93 Suppl 1, January <strong>2010</strong>, Pages S147-156<br />
Document Type : Article<br />
Keywords : C reactive protein; adult, aged, article, atherosclerosis, chronic kidney failure, echography, female, follow<br />
up, hospitalization, human, inflammation, male, malnutrition, methodology, middle aged, morbidity, mortality, nutritional<br />
status, outcome assessment, receiver operating characteristic, renal replacement therapy, Thailand;<br />
Adult, Aged, Atherosclerosis, C-Reactive Protein, Female, Follow-Up Studies, Hospitalization, Humans, Inflammation, Kidney<br />
Failure, Chronic, Male, Malnutrition, Middle Aged, Morbidity, Nutritional Status, Outcome Assessment (Health Care), Renal<br />
Dialysis, ROC Curve, Thailand<br />
<strong>Abstract</strong> : BACKGROUND: Malnutrition-Inflammation Score (MIS) has been proposed as a new quantitative system for assessment <strong>of</strong><br />
malnutrition and inflammation, which are common important risk factors for increased morbidity and mortality in<br />
maintenance hemodialysis (MHD) patients. OBJECTIVE: To determine the MIS and related it to the presence <strong>of</strong> atherosclerosis,<br />
and the morbidity and mortality rate. MATERIAL AND METHOD: The inflammatory and nutritional status in 100 MHD patients<br />
was evaluated by serum high-sensitivity C-reactive protein (hs-CRP), Subjective Global Assessment (SGA), and MIS.<br />
Atherosclerosis was defined by a history <strong>of</strong> cardiovascular disease or presence <strong>of</strong> carotid plaque by B-mode ultrasonography.<br />
Twelve-month prospective hospitalization and mortality rates were recorded. RESULTS: The MIS score was significantly higher<br />
in patients with atherosclerosis (5.5 +/- 2.3 vs. 3.0 +/- 1.7, p = 0.003) and modestly correlated with serum ferritin level (r =<br />
0.304, p = 0.03), but did not correlated with hs-CRP. The SGA was not associated with hs-CRP level and atherosclerosis. Over<br />
a 12-month follow-up period, 4 patients died and 28 were hospitalized at least once. Compared to the survivor group, MIS in<br />
the deceased group was significantly higher (8.0 +/- 1.4 vs. 5.1 +/- 2.3, p = 0.01) while SGA, hs-CRP and other biochemical<br />
markers were not significantly different. The Receiver Operating Characteristics Curves for the prediction <strong>of</strong> 1-year mortality<br />
from the MIS score identified the optimal cut-<strong>of</strong>f value <strong>of</strong> 7.5 with sensitivity <strong>of</strong> 75% and specificity <strong>of</strong> 88%. There was no<br />
association between MIS or SGA and hospitalization. CONCLUSION: MIS is a useful tool for the assessment <strong>of</strong> malnutrition and<br />
inflammatory status. It is superior to the conventional SGA as a predictor <strong>of</strong> short-term outcome in MHD patients.<br />
No.1006<br />
Author (s) : Pitaksajjakul, Pannamthip; Lekcharoensuk, Porntippa; Upragarin, Narin; Barbas, Carlos F., III; Ibrahim, Madiha Salah; Ikuta,<br />
Kazuyoshi; Ramasoota, Pongrama<br />
Title : Fab MAbs specific to HA <strong>of</strong> influenza virus with H5N1 neutralizing activity selected from immunized chicken phage library<br />
Source : BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Volume: 395, Issue: 4, Pages: 496-501, DOI:<br />
10.1016/j.bbrc.<strong>2010</strong>.04.040, Published: MAY 14 <strong>2010</strong><br />
Document Type : Article<br />
Keywords : H5N1 virus; Phage display technology; Monoclonal antibody production<br />
<strong>Abstract</strong> : Hemagglutinin protein (HA) was considered to be the primary target for monoclonal antibody production. This protein not<br />
only plays an important role in viral infections, but can also be used to differentiate H5N1 virus from other influenza A<br />
viruses. Hence, for diagnostic and therapeutic applications, it is important to develop anti-HA monoclonal antibody (MAb) with<br />
high sensitivity, specificity, stability, and productivity. Nine unique Fab MAbs were generated from chimeric chicken/human<br />
Fab phage display library constructed from cDNA derived from chickens immunized with recombinant hemagglutinin protein<br />
constructed from H5N1 avian influenza virus (A/Vietnam/1203/04). The obtained Fab MAbs showed several characteristics for<br />
further optimization and development-three clones were highly specific to only H5N1 virus. This finding can be applied to the<br />
development <strong>of</strong> H5N1 diagnostic testing. Another clone showed neutralization activity that inhibited H5N1 influenza virus<br />
infection in Madin-Darby canine kidney (MDCK) cells. In addition, one clone showed strong reactivity with several <strong>of</strong> the<br />
influenza A virus subtypes tested. The conversion <strong>of</strong> this clone to whole IgG is a promising study for a cross-neutralization<br />
activity test. (C) <strong>2010</strong> Elsevier Inc. All rights reserved.<br />
No.1007<br />
Author (s) : Pithayanukul, P., Leanpolchareanchai, J., Bavovada, R.<br />
Title : Inhibitory effect <strong>of</strong> tea polyphenols on local tissue damage induced by snake venoms<br />
Source : Phytotherapy Research, Volume 24, Issue SUPPL. 1, January <strong>2010</strong>, Pages S56-S62<br />
Document Type : Article<br />
Keywords : Anti-snake venoms; Calloselasma rhodostoma; Camellia sinensis L.; Naja naja kaouthia; Necrosis; Plant polyphenols<br />
368
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : The methanolic extract <strong>of</strong> fresh tea leaves <strong>of</strong> Camellia sinensis L. (Theaceae) (CS) was assayed for its potential to inhibit<br />
enzymes with hydrolytic activity in Naja naja kaouthia Lesson (Elapidae) and Calloselasma rhodostoma Kuhl (Viperidae)<br />
venoms. These snake venom enzymes are responsible for the early effects <strong>of</strong> envenomation, such as local tissue damage<br />
and infl ammation. The CS extract inhibited phospholipase A2, proteases, hyaluronidase and L-amino acid oxidase in both<br />
venoms by in vitro neutralization and inhibited the hemorrhagic and the dermonecrotic activities <strong>of</strong> the venoms in vivo. It is<br />
suggested that the inhibitory potential <strong>of</strong> the CS extract against local tissue damage induced by snake venoms may be<br />
attributed to complexation and chelation between the venom proteins and the phenolic contents <strong>of</strong> the extract. Copyright ©<br />
2009 John Wiley & Sons, Ltd.<br />
No.1008<br />
Author (s) : Pithukpakorn, M., Chaowalit, N.<br />
Title : Clinical presentation and echocardiographic findings <strong>of</strong> Thai patients with Marfan syndrome.<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand = Chotmaihet thangphaet, Volume 93 Suppl 1, January <strong>2010</strong>, Pages S43-47<br />
Document Type : Article<br />
Keywords : adolescent, adult, aorta aneurysm, article, Asian, echocardiography, ectopia lentis, female, genetics, human, male, Marfan<br />
syndrome, middle aged, pedigree, Thailand; Adolescent, Adult, Aortic Aneurysm, Asian Continental Ancestry<br />
Group, Echocardiography, Ectopia Lentis, Female, Humans, Male, Marfan Syndrome, Middle Aged, Pedigree, Thailand, Young<br />
Adult<br />
<strong>Abstract</strong> : OBJECTIVE: Marfan syndrome is an inherited disorder with systemic connective tissue involvement. The condition is clinically<br />
diverse and extremely pleiotropic. Though several articles had been published, no data from Thai individuals has ever been<br />
reported. Here we presented clinical manifestations and echocardiographic findings <strong>of</strong> Thai patients with Marfan syndrome.<br />
MATERIAL AND METHOD: Clinical and echocardiographic data were collected from series <strong>of</strong> Thai index cases with diagnosis <strong>of</strong><br />
Marfan syndrome at Siriraj Hospital between 1995 and 2009. Demographic data, clinical presentations, and echocardiographic<br />
data were reviewed and analyzed. RESULTS: Fifty individuals and thirty echocardiograms were identified. Thirty eight<br />
individuals (76%) fulfilled clinical criteria for definite Marfan syndrome and twelve individuals (24%) were incomplete Marfan<br />
syndrome. The majority <strong>of</strong> those individuals had significant organ involvement including dilatation <strong>of</strong> ascending aorta (78%),<br />
ectopia lentis (54%), and skeletal involvement (96%). CONCLUSIONS: In summary, this is the first report <strong>of</strong> clinical<br />
manifestation and echocardiographic findings <strong>of</strong> Thai patients with Marfan syndrome.<br />
No.1009<br />
Author (s) : Pitisuttithum, P., Choopanya, K., Rerk-Ngnam, S.<br />
Title : HIV-vaccine Research and Development in Thailand: Evolution and challenges<br />
Source : Vaccine, Volume 28, Issue SUPPL. 2, 26 May <strong>2010</strong>, Pages B45-B49<br />
Document Type : Article<br />
Keywords : Challenges; HIV-vaccine trials; Thailand<br />
<strong>Abstract</strong> : Thailand has been committed to HIV-Vaccine Research and Development since the launch <strong>of</strong> its National Plan, in 1993. Series<br />
<strong>of</strong> phase I/II trials, and two phase III clinical trials, have been conducted. The first efficacy trial was conducted with a high-risk<br />
group, injecting drug users (IDUs), and the second involved communities in the Eastern-seaboard provinces <strong>of</strong> Thailand. Both<br />
involved similar challenges in terms <strong>of</strong> infrastructure strengthening, capacity building, implementing the trials into the existing<br />
health system, staffing, and commitments. However, there were different issues in recruitment process, follow up, social<br />
impact events and community involvement since the target participants were different. © 2009 Elsevier Ltd. All rights<br />
reserved.<br />
No.1010<br />
Author (s) : Pitisuttithum, P.; Rerks-Ngarm, S.; Bussaratid, V.; Dhitavat, J.; Maek-a-nantawat, W.; Pungpak, S.; Suntharasamai, P.; Vanijanonta,<br />
S.; Nitayaphan, S.; Kaewkungwal, J.; Benenson, M. W.; Chiu, J.; Robb, M. L.; Gurunathan, S.; Francis, D.; Stablein, D. M.; Kim, J.<br />
Title : Safety Pr<strong>of</strong>iles <strong>of</strong> ALVAC HIV and AIDSVAX Vaccines in the Phase III Community Based Trial in Thailand<br />
Source : AIDS RESEARCH AND HUMAN RETROVIRUSES, Volume: 26, Issue: 10, Pages: A93-A94, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
369
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.1011<br />
Author (s) : Pitsawong, W., Sucharitakul, J., Prongjit, M., Tan, T.-C., Spadiut, O., Haltrich, D., Divne, C., Chaiyen, P.<br />
Title : A conserved active-site threonine is important for both sugar and flavin oxidations <strong>of</strong> pyranose 2-oxidase<br />
Source : Journal <strong>of</strong> Biological Chemistry, Volume 285, Issue 13, 26 March <strong>2010</strong>, Pages 9697-9705<br />
Document Type : Article<br />
<strong>Abstract</strong> : Pyranose 2-oxidase (P2O) catalyzes the oxidation by O2 <strong>of</strong> D-glucose and several aldopyranoses to yield the 2-ketoaldoses<br />
and H 2O2. Based on crystal structures, in one rotamer conformation, the threonine hydroxyl <strong>of</strong> Thr169 forms H-bonds to the<br />
flavin-N5/O4 locus, whereas, in a different rotamer, it may interact with either sugar or other parts <strong>of</strong> the P2O·sugar complex.<br />
Transient kinetics <strong>of</strong> wild-type (WT) and Thr169 → S/N/G/A replacement variants show that D-Glc binds to T169S, T169N, and<br />
WT with the same Kd (45-47mM), and the hydride transfer rate constants (kred) are similar (15.3-9.7 s -1 at 4 °C ). kred <strong>of</strong><br />
T169G with D-glucose (0.7 s -1, 4 °C) is significantly less than that <strong>of</strong> WT but not as severely affected as in T169A (kred <strong>of</strong> 0.03<br />
s-1 at 25 °C). Transient kinetics <strong>of</strong> WT and mutants using D-galactose show that P2O binds D-galactose with a one-step binding<br />
process, different from binding <strong>of</strong> D-glucose. In T169S, T169N, and T169G, the overall turnover with D-Gal is faster than that <strong>of</strong><br />
WT due to an increase <strong>of</strong> kred. In the crystal structure <strong>of</strong> T169S, Ser169 Oγassumes a position identical to that <strong>of</strong> Oγ1 in<br />
Thr169; in T169G, solvent molecules may be able to rescue H-bonding. Our data suggest that a competent reductive halfreaction<br />
requires a side chain at position 169 that is able to form an H-bond within the ES complex. During the oxidative halfreaction,<br />
all mutants failed to stabilize a C4a-hydroperoxyflavin intermediate, thus suggesting that the precise position and<br />
geometry <strong>of</strong> the Thr169 side chain are required for intermediate stabilization. © <strong>2010</strong> by The American Society for<br />
Biochemistry and Molecular Biology, Inc.<br />
Keywords : Binding process, D-galactose, D-glucose, Flavin oxidation, H-bonding, H-bonds, Hydride transfer rates, Precise<br />
position, Pyranose, Rotamers, Side chains, Solvent molecules, Transient kinetics, Wild types;<br />
Glucose, Oxidation, Phosphatases, Rate constants, Reaction intermediates, Sugar (sucrose); Crystal structure; flavine<br />
nucleotide, galactose, glucose, oxidoreductase, pyranose 2 oxidase, sugar, threonine, unclassified<br />
drug, carbohydrate, hydrogen peroxide, oxygen, pyranose oxidase, rib<strong>of</strong>lavin derivative; amino acid sequence, article, binding<br />
kinetics, complex formation, controlled study, crystal structure, enzyme active site, enzyme binding, hydrogen<br />
bond, oxidation, priority journal, wild type, chemistry, enzymology, kinetics, methodology, site directed<br />
mutagenesis, temperature, Trametes, X ray crystallography; Carbohydrate Dehydrogenases, Carbohydrates, Catalytic<br />
Domain, Crystallography, X-Ray, Flavins, Galactose, Glucose, Hydrogen Bonding, Hydrogen Peroxide, Kinetics, Mutagenesis, Site-<br />
Directed, Oxygen, Temperature, Threonine, Trametes<br />
No.1012<br />
Author (s) : Piwpankaew, Y., Monteerarat, Y., Suptawiwat, O., Puthavathana, P., Uipresertkul, M., Auewarakul, P.<br />
Title : Distribution <strong>of</strong> viral RNA, sialic acid receptor, and pathology in H5N1 avian influenza patients<br />
Source : APMIS, Volume 118, Issue 11, November <strong>2010</strong>, Pages 895-902<br />
Document Type : Article<br />
Keywords : H5N1 avian influenza virus; In situ hybridization; Sialic acid receptor; Viral RNA<br />
<strong>Abstract</strong> : Avian influenza virus H5N1 infects and causes severe disease in humans. Despite the increasing number <strong>of</strong> fatal cases in 15<br />
countries and the threat <strong>of</strong> influenza pandemic, detailed pathologic and virologic findings in humans are limited. In this study,<br />
we describe histopathologic findings, distributions <strong>of</strong> viral RNA and the Neu5Acα2-3Galβ1-4 sialic acid receptor in human<br />
tissues from three fatal avian influenza cases. Many <strong>of</strong> our findings are in agreement with previously published autopsy<br />
studies, that is, viral RNA and receptor were detected in type II pneumocytes, tracheal epithelium, intestinal epithelium,<br />
spleen, lymph nodes, and Kupffer's cells. In addition, we detected the viral RNA in glomeruli and renal tubules. There was a<br />
good correlation between viral RNA distribution and the presence <strong>of</strong> the sialic acid receptor. Despite the wide distribution <strong>of</strong><br />
viral RNA, tissue damage and inflammation were mainly limited to pulmonary tissue. © <strong>2010</strong> The Authors. Journal<br />
Compilation © <strong>2010</strong> APMIS.<br />
No.1013<br />
Author (s) : Piyaphanee, W., Shantavasinkul, P., Phumratanaprapin, W., Udomchaisakul, P., Wichianprasat, P., Benjavongkulchai, M., Ponam,<br />
T., Tantawichian, T.<br />
Title : Rabies exposure risk among foreign backpackers in Southeast Asia<br />
Source : American Journal <strong>of</strong> Tropical Medicine and Hygiene, Volume 82, Issue 6, June <strong>2010</strong>, Pages 1168-1171<br />
Document Type : Article<br />
370
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : rabies vaccine; adolescent, adult, aged, article, bite, Caucasian, cross-sectional study, environmental<br />
exposure, female, human, incidence, infection risk, licking, major clinical study, male, medical<br />
information, questionnaire, rabies, Rabies virus, Southeast Asia, travel, vaccination; Adolescent, Adult, Animals, Asia,<br />
Southeastern, Cross-Sectional Studies, Dog Diseases, Dogs, Health Knowledge, Attitudes, Practice, Humans, Middle<br />
Aged, Questionnaires, Rabies, Rabies Vaccines, Risk Factors, Travel, Travel Medicine, Young Adult<br />
<strong>Abstract</strong> : Rabies remains a problem in Southeast Asia where large numbers <strong>of</strong> backpackers visit each year. During May-June 2008, a<br />
survey study was conducted <strong>of</strong> foreign backpackers in Bangkok, Thailand to assess their risk <strong>of</strong> rabies exposure. Eight hundred<br />
seventy (870) questionnaires were collected and analyzed. The median age <strong>of</strong> the backpackers was 25.5 years. Most <strong>of</strong> them<br />
were European (68.4%), followed by North American (13.2%). Although 80.7% had sought health information before traveling,<br />
only 55.6% had received information about rabies. Only 18.1% had completed pre-exposure rabies vaccination (3 shots)<br />
before travel, whereas 70.9% had not been vaccinated for rabies at all. In this study, the incidence <strong>of</strong> being licked was 3.56%,<br />
and <strong>of</strong> being bitten 0.69%, on average stays <strong>of</strong> 30.06 days in Southeast Asia. More than a half (54%) <strong>of</strong> exposures occurred in<br />
the first 10 days after arrival in Southeast Asia. Copyright © <strong>2010</strong> by The American Society <strong>of</strong> Tropical Medicine and Hygiene.<br />
No.1014<br />
Author (s) : Piyaphanee, W.; Kusolsuk, T.; Kittitrakul, C.; Suttithum, W.; Ponam, T.; Wilairatana, P.<br />
Title : Incidence and impact <strong>of</strong> travelers' diarrhea among foreign backpackers in Southeast Asia<br />
Source : INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, Volume: 14, Supplement: 1, Pages: E133-E133, DOI:<br />
10.1016/j.ijid.<strong>2010</strong>.02.1779, Published: MAR <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1015<br />
Author (s) : Piyasirisilp, S., Premprawat, W., Thamlikitkul, V.<br />
Title : Therapeutic equivalence <strong>of</strong> generic imipenem/cilastatin for therapy <strong>of</strong> infections at Siriraj Hospital.<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand = Chotmaihet thangphaet, Volume 93 Suppl 1, January <strong>2010</strong>, Pages S117-125<br />
Document Type : Article<br />
Keywords : antiinfective agent, cilastatin, generic drug, imipenem; adult, aged, article, bacterial infection, comparative study, cross<br />
infection, female, hospitalization, human, male, medical record, middle aged, pharmacokinetics, Thailand, treatment<br />
outcome, university hospital; Adult, Aged, Aged, 80 and over, Anti-Bacterial Agents, Bacterial Infections, Cilastatin, Cross<br />
Infection, Drugs, Generic, Female, Hospitalization, Hospitals, <strong>University</strong>, Humans, Imipenem, Male, Medical Records, Middle<br />
Aged, Thailand, Therapeutic Equivalency, Treatment Outcome<br />
<strong>Abstract</strong> : BACKGROUND: Several generic imipenem/cilastatin formulations have been approved by Thai FDA and a generic<br />
imipenem/cilastatin (Yungjin) has been available in SirirajHospital since 2007. Since imipenem/cilastatin is usually given to the<br />
patients with serious hospital-acquired infections, the generic imipenem/cilastatin must be therapeutically equivalent to the<br />
original imipenem/cilastatin. The objective <strong>of</strong> the study was to compare effectiveness and safety <strong>of</strong> generic<br />
imipenem/cilastatin with original imipenem/cilastatin for therapy <strong>of</strong> infections in hospitalized patients at Siriraj Hospital.<br />
MATERIAL AND METHOD: Medical records <strong>of</strong> adult hospitalized patients at Siriraj Hospital who received imipenem/cilastatin at<br />
least 48 hours during June 2007 to September 2008 were reviewed. The effectiveness data <strong>of</strong> 300 patients who received<br />
original imipenem/cilastatin were compared with those <strong>of</strong> 300 patients who received generic imipenem/cilastatin in order to<br />
determine if a difference in composite favorable outcome <strong>of</strong> both formulations was within 10%. RESULTS: The demographics,<br />
clinical features <strong>of</strong> infections, site <strong>of</strong> infections, type <strong>of</strong> causative organisms and concomitant antibiotics <strong>of</strong> the patients in<br />
both groups were not significantly different. The overall favorable outcomes in the original imipenem/cilastatin and the<br />
generic imipenem/cilastatin groups were 65% and 58.7% respectively (absolute difference 6.3%, 95% CI -1.4% to 14%). Cure<br />
rates <strong>of</strong> infections in the original imipenem/cilastatin and the generic imipenem/cilastatin groups were 35% and 28.7%<br />
respectively (absolute difference 6.3%, 95% CI -1.1% to 13.7%). Super-infection rates in the original imipenem/cilastatin and<br />
the generic imipenem/cilastatin groups were 4.7% and 9% respectively (absolute difference -4.3%, 95% CI -8.5% to 0.3%).<br />
Mortality due to infections in the original imipenem/cilastatin and the generic imipenem/cilastatin groups were 18.3% and<br />
21.3% respectively (absolute difference -3%, 95% CI -9.4% to 3.4%). Overall mortality in the original imipenem/cilastatin and<br />
the generic imipenem/cilastatin groups were 35.3% and 43% respectively (absolute difference -7.7%, 95% CI -15.3% to 0.1%).<br />
The occurrence <strong>of</strong> adverse events in the patients in both groups was not significantly different. CONCLUSION: Although the<br />
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point estimate <strong>of</strong> composite favorable outcome <strong>of</strong> the patients who received generic imipenem/cilastatin (Yungjin) was <<br />
10% <strong>of</strong> those who received original imipenem/cilastatin (Tienam), generic imipenem/cilastatin showed a trend for therapeutic<br />
non-equivalence to original imipenem/cilastatin because the upper limits <strong>of</strong> 95% confidence interval <strong>of</strong> differences <strong>of</strong> several<br />
important clinical outcomes were more than 10%.<br />
No.1016<br />
Author (s) : Piyatanont, K., Bamrungrak, K., Watcharananan, S., Sathapatayavongs, B., Chuncharunee, S., Larbcharoensub, N., Witoonpanich,<br />
R.<br />
Title : Intravascular B-cell lymphoma presenting with cauda equina syndrome: The role <strong>of</strong> skin biopsy<br />
Source : European Journal <strong>of</strong> Dermatology, Volume 20, Issue 6, November <strong>2010</strong>, Pages 821-822<br />
Document Type : Letter<br />
Keywords : methotrexate; aged, anamnesis, B cell lymphoma, bone marrow biopsy, case report, cauda equina syndrome, clinical<br />
feature, drug megadose, echography, histopathology, human, human tissue, intravascular b cell<br />
lymphoma, letter, male, neurologic examination, nuclear magnetic resonance imaging, pancytopenia, skin biopsy;<br />
Aged, Biopsy, Diagnostic Imaging, Fatal Outcome, Humans, Lymphoma, Large B-Cell, Diffuse, Male, Polyradiculopathy, Vascular<br />
Neoplasms<br />
<strong>Abstract</strong> : –<br />
No.1017<br />
Author (s) : Piyawinijwong, S., Sirisathira, N.<br />
Title : Supernumerary subclavius muscle in Thais: Predisposing cause <strong>of</strong> thoracic outlet syndrome<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 9, September <strong>2010</strong>, Pages 1065-1069<br />
Document Type : Article<br />
Keywords : Subclavius muscle; Subclavius posticus; Supernumerary; Thoracic outlet syndrome<br />
<strong>Abstract</strong> : Objective: To demonstrate and classify the variation <strong>of</strong> the subclavius muscle according to its insertion in the Thais. Material<br />
and Method: One hundred and twenty eight upper limbs were dissected out to expose the scapular region. The attachments<br />
<strong>of</strong> subclavius muscles were examined and recorded. Results: The subclavius muscle was categorized into 4 types according to<br />
its insertion. There are 64.06% <strong>of</strong> type I, 17.96% <strong>of</strong> type II, 15.62% <strong>of</strong> type III and 2.34% <strong>of</strong> type IV. The insertion <strong>of</strong> subclavius<br />
muscle is gradually extended from the shallow groove on the inferior surface <strong>of</strong> the clavicle towards the conoid ligament and<br />
corocoid process, to the superior transverse scapular ligament and to the superior border <strong>of</strong> the scapula adjacent to the<br />
insertion <strong>of</strong> inferior belly <strong>of</strong> omohyoid muscle. Conclusion: The present study prevailed 64% normal subclavius muscle and<br />
other 36% <strong>of</strong> varied supernumerary subclavius muscle. The presence <strong>of</strong> supernumerary subclavius muscle could be a<br />
predisposing causative factor <strong>of</strong> thoracic outlet syndrome.<br />
No.1018<br />
Author (s) : Plasen, D., Akarasereenont, P., Techatraisak, K., Chotewuttakorn, S., Thaworn, A.<br />
Title : Initial response <strong>of</strong> endothelial cells to acute stimulation with a lipid component: increase cyclooxygenase activity by<br />
induction <strong>of</strong> COX-2 through activation <strong>of</strong> tyrosine kinase.<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand = Chotmaihet thangphaet, Volume 93 Suppl 2, February <strong>2010</strong>, Pages S59-67<br />
Document Type : Article<br />
Keywords : 25 hydroxycholesterol, 25-hydroxycholesterol, 6 oxoprostaglandin F1 alpha, antihypertensive agent, arachidonic acid<br />
derivative, cholesterol derivative, cyclooxygenase 2, genistein, prostacyclin, prostaglandin synthase, protein kinase<br />
inhibitor, protein tyrosine kinase, PTGS2 protein, human; article, biosynthesis, cytology, dose response, drug<br />
effect, endothelium cell, enzyme immunoassay, enzymology, human, metabolism, newborn, time, umbilical vein, vascular<br />
endothelium, Western blotting; 6-Ketoprostaglandin F1 alpha, Antihypertensive Agents, Arachidonic Acids, Blotting,<br />
Western, Cyclooxygenase 2, Dose-Response Relationship, Drug, Endothelial Cells, Endothelium,<br />
Vascular, Epoprostenol, Genistein, Humans, Hydroxycholesterols, Immunoenzyme Techniques, Infant, Newborn, Prostaglandin-<br />
Endoperoxide Synthases, Protein Kinase Inhibitors, Protein-Tyrosine Kinases, Time Factors, Umbilical Veins<br />
<strong>Abstract</strong> : To study the initial response <strong>of</strong> endothelial cells acutely stimulated with a lipid component in the aspect <strong>of</strong> cyclooxygenase<br />
(COX) function which needed for prostacyclin synthesis, an endogenous antiatherogenic agent secreted from endothelial<br />
cells. 25 hydroxycholesterol (25OHC) was used as a representative lipid component for stimulating human umbilical vein<br />
endothelial cell (HUVEC) obtained from umbilical cords <strong>of</strong> healthy newborns with informed consent <strong>of</strong> their mothers. HUVEC<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
were treated with 25OHC (0.1, 1 or 10 microgram/mL) at times 6, or 24 h. COX activity was measured from amount <strong>of</strong> 6-keto-<br />
PGF(1 alfa) production in the presence <strong>of</strong> exogenous arachidonic acids (10 micromolar; 10 min) by enzyme immunoassay. The<br />
amount <strong>of</strong> COX-1 and COX-2 protein were detected by Western blot. Cell viability was assessed by using MTT assay. 25OHC<br />
induced COX-2 protein production with increasing the activity <strong>of</strong> COX enzyme in HUVEC without change in amount <strong>of</strong> COX-1<br />
protein. The induction <strong>of</strong> COX-2 or increasing in COX activity depended on concentration <strong>of</strong> 25OHC and time to exposure<br />
which seemed to be inhibited by genistein, a specific tyrosine kinase inhibitor. Acute stimulation <strong>of</strong> HUVEC with 25OHC, an<br />
atherosclerotic lipid component, increases the activity <strong>of</strong> COX by inducing COX-2 expression in a manner that depended on<br />
concentration and time. The induction <strong>of</strong> COX-2 expression might possibly mediated through activation protein tyrosine<br />
kinase. These responses may be an initial defensive mechanism <strong>of</strong> endothelial cells from lipid component attack.<br />
No.1019<br />
Author (s) : Pohl, P., Dural, J., Vorapalawut, N., Merdrignac, I., Lienemann, C.P., Carrier, H., Grassl, B., Bouyssiere, B., Lobinski, R.<br />
Title : Multielement molecular size fractionation in crude oil and oil residue by size exclusion microchromatography with high<br />
resolution inductively coupled plasma mass spectrometric detection (HR ICP MS)<br />
Source : Journal <strong>of</strong> Analytical Atomic Spectrometry, Volume 25, Issue 12, December <strong>2010</strong>, Pages 1974-1977<br />
Document Type : Article<br />
Keywords : Certified reference materials, Existing method, Flow injection, High resolution, High sensitivity, HR-ICP-MS, Mass spectrometric<br />
detection, Microchromatography, Molecular mass fractions, Molecular size fractionation, Multi-element, Multielement<br />
determination, Oil residues, Size exclusion, Vacuum distillation; Chromatographic analysis, Chromium, Crude<br />
oil, Distillation, Electromagnetic induction, Inductively coupled plasma; Mass spectrometers<br />
<strong>Abstract</strong> : Microchromatography using permeation through gels with the increasing exclusion limit (5000, 400000 and 20000000 Da) was<br />
combined with high resolution (R = 4000) ICP MS to measure the distribution <strong>of</strong> Co, Cr, Fe, Ni, S, Si, V and Zn as a function <strong>of</strong><br />
the molecular mass fraction <strong>of</strong> crude oil and oil vacuum distillation residue. The accuracy <strong>of</strong> the simultaneous multielement<br />
determination was verified by the flow-injection-ICP MS analysis <strong>of</strong> certified reference materials (wear-metals in lubricating<br />
oils: SRM 1084a and SRM 1085b). The method allowed the acquisition <strong>of</strong> chromatograms with high sensitivity competitive<br />
existing methods, showing element- and sample origin-dependent morphology. © <strong>2010</strong> The Royal Society <strong>of</strong> Chemistry.<br />
No.1020<br />
Author (s) : Pohl, P., Vorapalawut, N., Bouyssiere, B., Carrier, H., Lobinski, R.<br />
Title : Direct multi-element analysis <strong>of</strong> crude oils and gas condensates by double-focusing sector field inductively coupled plasma<br />
mass spectrometry (ICP MS)<br />
Source : Journal <strong>of</strong> Analytical Atomic Spectrometry, Volume 25, Issue 5, <strong>2010</strong>, Pages 704-709<br />
Document Type : Article<br />
Keywords : Carbon rich, Certified reference materials, Detection limits, Different origins, Double focusing, External<br />
calibration, matrix, Multielement analysis, Oil samples, Order <strong>of</strong> magnitude, Organic solutions, Polyatomic<br />
interferences, Quadrupoles, Sector field, Sector field inductively coupled plasma mass spectrometries, Simultaneous<br />
determinations, Trace element analysis; Aromatic hydrocarbons, Barium, Calcium, Chromium, Electromagnetic induction, Gas<br />
oils, Inductively coupled plasma, Inductively coupled plasma mass spectrometry, Manganese, Manganese compounds, Metal<br />
analysis, Silver, Tin, Trace analysis, Trace elements, Xylene; Gas condensates<br />
<strong>Abstract</strong> : A double-focusing sector field ICP MS was optimized for the direct simultaneous determination <strong>of</strong> Ag, Al, Ba, Ca, Cd, Co, Cr,<br />
Cu, Fe, Mg, Mn, Mo, Ni, Pb, Sn, Ti, V in organic solutions. Polyatomic interferences originating from the carbon-rich matrix were<br />
completely eliminated at a resolution <strong>of</strong> 4000 allowing the detection limits at the low pg g-1 level to be obtained (typically<br />
one order <strong>of</strong> magnitude lower than using a quadrupole ICP MS). A method for the routine comprehensive trace element<br />
analysis <strong>of</strong> xylene solutions <strong>of</strong> oil samples using external calibration was developed. It was validated by the analysis <strong>of</strong> three<br />
certified reference materials and applied to the analysis <strong>of</strong> 2 gas condensate samples and 4 oil samples <strong>of</strong> different origins. ©<br />
The Royal Society <strong>of</strong> Chemistry <strong>2010</strong>.<br />
No.1021<br />
Author (s) : Pohl, P., Vorapalawut, N., Bouyssiere, B., Lobinski, R.<br />
Title : Trace-level determination and insight in speciation <strong>of</strong> silicon in petrochemical samples by flow-injection high resolution ICP<br />
MS and HPLC-high resolution ICP MS<br />
Source : Journal <strong>of</strong> Analytical Atomic Spectrometry, Volume 25, Issue 9, September <strong>2010</strong>, Pages 1461-1466<br />
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Document Type : Conference Paper<br />
Keywords : Calibration graphs, Chemical forms, Detection limits, Double focusing, Flow injection, High-resolution ICP-MS, Magnetic<br />
sectors, Micro-flow, Normal phase HPLC, Orders <strong>of</strong> magnitude, Organic solutions, Related matrices, Sample dilution, Sample<br />
introduction, Sample matrix, Signal-to-background ratio, Spray chamber, Standard samples, Trace-level determination; Trace<br />
analysis; Silicon compounds<br />
<strong>Abstract</strong> : A method for the determination <strong>of</strong> silicon in organic solutions was developed using a double focusing magnetic sector ICP MS<br />
operating at a resolution <strong>of</strong> 4000. The signal-to-background ratio was improved 10-fold over standard sample introduction<br />
systems by the use <strong>of</strong> micro-flow injection total consumption sample introduction. The detection limits down to 1 ng g -1,<br />
the calibration graph linear over 4 orders <strong>of</strong> magnitude and precision to within a few percent were obtained. The effects <strong>of</strong><br />
the sample matrix and <strong>of</strong> the chemical form <strong>of</strong> silicon on the sensitivity were investigated and alleviated when necessary by<br />
heating the spray chamber and sample dilution. Normal phase HPLC-ICP MS and size-exclusion ICP MS were proposed to gain<br />
an insight into the purity <strong>of</strong> the silicon standard compounds, their reactivity with different petroleum-related matrices and<br />
speciation <strong>of</strong> silicon. © <strong>2010</strong> The Royal Society <strong>of</strong> Chemistry.<br />
No.1022<br />
Author (s) : Poljaroen, J., Vanichviriyakit, R., Tinikul, Y., Phoungpetchara, I., Linthong, V., Weerachatyanukul, W., Sobhon, P.<br />
Title : Spermatogenesis and distinctive mature sperm in the giant freshwater prawn, Macrobrachium rosenbergii (De Man, 1879)<br />
Source : Zoologischer Anzeiger, Volume 249, Issue 2, August <strong>2010</strong>, Pages 81-94<br />
Document Type : Article<br />
Keywords : Basic nuclear proteins; Chromatin condensation; Macrobrachium rosenbergii; Spermatogenesis; Spermiogenesis; Testis;<br />
Ultrastructure<br />
<strong>Abstract</strong> : The structures <strong>of</strong> differentiating male germ cells in the testis <strong>of</strong> the giant freshwater prawn, Macrobrachium rosenbergii, were<br />
studied by light and electron microscopy. Based on ultrastructural characteristics, the developing male germ cells are<br />
classified into 12 stages, including spermatogonia, six phases <strong>of</strong> primary spermatocytes (leptotene, zygotene, pachytene,<br />
diplotene, diakinesis and metaphase), secondary spermatocyte, three stages <strong>of</strong> spermatids and mature sperm. During<br />
spermatogenesis, the differentiating germ cells have characteristics similar to those <strong>of</strong> other invertebrates, but they exhibit<br />
some unique characteristics during spermiogenesis. In particular, an early spermatid has a round nucleus with highly<br />
condensed heterochromatin, appearing as thick interconnecting cords throughout the nucleus. In contrast to most<br />
invertebrates and vertebrates, the chromatin begins to decondense in one-half <strong>of</strong> the nucleus at the mid spermatid stage. In<br />
the late spermatid, the chromatin becomes almost entirely decondensed with only a small crescent-shaped heterochromatin<br />
patch remaining at the anterior pole <strong>of</strong> the nucleus. Mature sperm possess an everted umbrella-shaped plate with a spike<br />
covering the anterior pole <strong>of</strong> the nucleus, whose chromatin is totally decondensed as only small traces <strong>of</strong> histones H3 and<br />
H2B remain. The acrosome appears at the ruffled border <strong>of</strong> the spike plate as small sac-like structures. Few mitochondria<br />
remain in the cytoplasm at the posterior pole. © <strong>2010</strong> Elsevier GmbH.<br />
No.1023<br />
Author (s) : Polsomboon, S.; Tainchum, K.; Sungvornyothin, S.; Suwonkerd, W.; Achee, N.L.; Grieco, J.P.; Chareonviriyaphap, T.<br />
Title : DIFFERENTIAL BEHAVIORAL RESPONSES OBSERVED IN AEDES AEGYPTI IN RESPONSE TO REDUCED COVERAGE AND DOSE OF<br />
STANDARD VECTOR CONTROL CHEMICALS IN THAILAND<br />
Source : AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, Volume: 83, Issue: 5, Supplement: S, Pages: 53-53, Meeting<br />
<strong>Abstract</strong>: 176, Published: NOV <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1024<br />
Author (s) : Pongcharoen, P.; Tohtong, R.<br />
Title : THE ROLE OF CD44 IN HUMAN CHOLANGIOCARCINOMA CELLS<br />
Source : ANNALS OF ONCOLOGY, Volume: 21, Supplement: 6, Pages: 54-55, Published: <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
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No.1025<br />
Author (s) : Pongkumpai, M., Trakulsomboon, S., Suankratay, C.<br />
Title : An evaluation <strong>of</strong> 2.0 McFarland Etest method for detection <strong>of</strong> heterogeneous vancomycin-intermediate Staphylococcus<br />
aureus<br />
Source : Asian Biomedicine, Volume 4, Issue 1, February <strong>2010</strong>, Pages 141-145<br />
Document Type : Article<br />
Keywords : Etest; Heterogeneous vancomycin-intermediate S. aureus; Population analysis pr<strong>of</strong>iles; Staphylococcus<br />
aureus; Susceptibility<br />
<strong>Abstract</strong> : Background: Staphylococcus aureus with reduced susceptibility to vancomycin or heterogeneous vancomycinintermediate S.<br />
aureus (hVISA) have become increasingly reported from various parts <strong>of</strong> the world. hVISA cannot be detected by routine test<br />
for minimal inhibitory concentration (MIC) for vancomycin. The gold standard method for detection, population analysis<br />
pr<strong>of</strong>iles (PAP) method, is complicated, time-consuming, expensive, and needs well-trained microbiologists. Objective: Evaluate<br />
<strong>of</strong> 2.0 McFarland Etest method, in comparison with the PAP method, for detection <strong>of</strong> hVISA in clinical specimens. Methods:<br />
All methicillin-resistant S. aureus strains from clinical specimens isolated from consecutive patients attended at King<br />
Chulalongkorn Memorial Hospital and Siriraj Hospital, Bangkok between 2006 and 2007 were studied. 1 hundred nineteen<br />
specimens were obtained. The PAP method detected six hVISA strains 5 from blood and from cultures) from four patients at<br />
King Chulalongkorn Memorial Hospital, accounting for a prevalence <strong>of</strong> 6.35%. The MIC determined by agar dilution method<br />
was in the range <strong>of</strong> 2-3 ìg/mL. Results: 2.0 McFarland Etest method detected no false positive and five false negatives (42%),<br />
and gave a sensitivity and a specificity <strong>of</strong> 16.7% and 100%, respectively. The one-point population analysis screening method<br />
detected two false positives and 1 false negative, and gave a sensitivity <strong>of</strong> 83.3% and a specificity and 98.2%. Conclusion: The<br />
2.0 McFarland Etest method had a very good specificity but a poor sensitivity for detecting hVISA. It may be used as an<br />
alternative method to confirm detection <strong>of</strong> hVISA.<br />
No.1026<br />
Author (s) : Pongpech, P., Amornnopparattanakul, S., Panapakdee, S., Fungwithaya, S., Nannha, P., Dhiraputra, C., Leelarasamee, A.<br />
Title : Antibacterial activity <strong>of</strong> carbapenem-based combinations againts multidrug-resistant Acinetobacter baumannii<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 2, February <strong>2010</strong>, Pages 161-171<br />
Document Type : Article<br />
Keywords : Acinetobacter baumannii; Antimicrobial combination; Colistin; Imipenem; Meropenem; Multidrugresistance;<br />
Sulbactam; Synergy<br />
<strong>Abstract</strong> : Background: Multidrug-resistant (MDR) Acinetobacter baumannii are increasingly encountered and frequently susceptible only<br />
to colistin with their MIC values close to resistance breakpoint. Antibacterial activity <strong>of</strong> two carbapenem-based combinations<br />
were explored in order to overcome the bacterial resistance. Material and Method: Thirty clinical isolates <strong>of</strong> MDR A.<br />
baumannii were employed to assess in vitro antibacterial activity <strong>of</strong> two carbapenem-based regimens. Imipenem combined<br />
with colistin and meropenem combined with colistin and sulbactam were the first and second regimens, respectively. All<br />
isolates were resistant to imipenem (MIC range: 8-128 ?g/ml) and meropenem (MIC range: 64-256 μg/ml) but still susceptible<br />
to colistin (MIC range: 0.5-2 μg/ml). The MIC range <strong>of</strong> sulbactam was 4-64 μg/ml. None <strong>of</strong> the isolates produced metallo-βlactamase.<br />
Results: Synergistic antibacterial effect <strong>of</strong> imipenem combined with colistin was observed against 100 percent <strong>of</strong> A.<br />
baumannii isolates by the checkerboard microdilution panel method. In a subsequent time kill study, the most active<br />
concentration <strong>of</strong> this regimen was the combination <strong>of</strong> imipenem at the fixed concentration <strong>of</strong> 32 μg/ml and colistin at the<br />
1/4 <strong>of</strong> the MIC values <strong>of</strong> each isolate that exerted significantly higher bactericidal activity than imipenem at 32 μg/ml alone<br />
and colistin alone at the 1/4 <strong>of</strong> the MIC values. The scanning electron micrographs demonstrated major cell morphological<br />
change and cell wall destruction after 2-hour exposure to this combination. The triple combinations <strong>of</strong> meropenem,<br />
sulbactam and colistin showed synergy against 96.7 percent <strong>of</strong> MDR A. baumannii while double combinations <strong>of</strong> either<br />
meropenem and sulbactam, meropenem and colistin, and sulbactam and colistin showed synergy effects <strong>of</strong> 70%, 73.3% and<br />
53.3%, respectively. The time kill study using ten isolates also showed better killing effect by the triple combination than any<br />
<strong>of</strong> the double combinations. Conclusion: Antibacterial activity against MDR A. baumannii <strong>of</strong> imipenem plus colistin was<br />
superior over any single <strong>of</strong> the two agents. The addition <strong>of</strong> sulbactam to meropenem and colistin may further improve their<br />
antibacterial activity. The double or triple carbapenem-based combinations <strong>of</strong>fer promising alternatives in the treatment <strong>of</strong><br />
infections due to MDR A. baumannii.<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.1027<br />
Author (s) : Pongpech, S., Aurboonyawat, T., Visudibhan, A., Jiarakongmun, P.<br />
Title : Endovascular management in children with vein <strong>of</strong> Galen aneurysmal malformation<br />
Source : Minimally Invasive Neurosurgery, Volume 53, Issue 4, <strong>2010</strong>, Pages 169-174<br />
Document Type : Article<br />
Keywords : choroidal type; embolization; mural type; vein <strong>of</strong> galen aneurysmal malformation<br />
<strong>Abstract</strong> : Background: A vein <strong>of</strong> Galen aneurysmal malformation (VGAM) is a relatively rare vascular malformation, <strong>of</strong>ten resulting in<br />
high morbidity and mortality. While surgical arterial clipping has been reported for decades, results in the literature have<br />
recently favored endovascular treatment. Methods: During a 10-year period, all children who were diagnosed with VGAM were<br />
included in our follow-up study. Clinical and radiological records <strong>of</strong> 5 consecutive patients were reviewed. Results: 5 children<br />
(4 infants and 1 child) who suffered from symptoms caused by VGAM were treated by means <strong>of</strong> transarterial embolization<br />
with n-butyl cyanoacrylate (NBCA) alone. Their age at the time <strong>of</strong> diagnosis ranged from 4 months to 3 years. 4 <strong>of</strong> the 5<br />
patients presented with macrocranium, and 3 <strong>of</strong> those 4 patients were infants. The fifth patient presented with seizures. None<br />
<strong>of</strong> the patients presented with hemorrhage. 2 <strong>of</strong> the 5 VGAM patients were classified as the mural type, while the others 3<br />
were <strong>of</strong> the choroidal type. Both mural type patients achieved total obliteration <strong>of</strong> lesions with good outcomes. One <strong>of</strong> the<br />
remaining 3 patients exhibited autistic behavior during late follow-up, whereas the other 2 had good outcomes. 2 patients<br />
suffered from asymptomatic ruptured arterial feeders during embolization, which were treated immediately with glue<br />
embolization. Conclusion: Our purpose in treating a patient with VGAM is to achieve normal brain development using<br />
minimally invasive interventions. Our treatment strategies were influenced by each patient's clinical status, their ages, and<br />
varying radiographic features. We achieved considerable successful in treating our patients by means <strong>of</strong> transarterial<br />
embolization alone. © Georg Thieme Verlag KG Stuttgart - New York.<br />
No.1028<br />
Author (s) : Pongpruttipan, T., Treetipsatit, J., Amornpichetkul, K., Kummalue, T., Chuphrom, A., Khuhapinant, A., Siritanaratkul, N.,<br />
Sukpanichnant, S.<br />
Title : EBV-positive diffuse large B-cell lymphoma <strong>of</strong> the elderly with dual genotype and aberrant TIA-1 expression: A case report<br />
and review <strong>of</strong> literature<br />
Source : Journal <strong>of</strong> Hematopathology,<strong>2010</strong>, 3 (2), pp. 83 – 89.<br />
Document Type : Article<br />
Keywords : Cytotoxic protein; Diffuse large B-cell lymphoma; EBV; Immunoglobulin heavy chain gene; T-cell receptor gene; TIA-1<br />
<strong>Abstract</strong> : B-cell lymphomas generally do not express T-cell intracellular antigen-1 (TIA-1), a cytotoxic granule-associated protein. We<br />
report a case <strong>of</strong> EBV-positive diffuse large B-cell lymphoma (DLBCL) <strong>of</strong> the elderly with aberrant expression <strong>of</strong> TIA-1. The<br />
patient was an 84-year-old man with a history <strong>of</strong> progressively enlarged left parotid mass and mild facial paresis for 2 months.<br />
The incisional biopsy revealed sheets <strong>of</strong> medium to large lymphoma cells with focal angiocentricity and extensive tumor<br />
necrosis; only a few scattered small lymphocytes were noted among the lymphoma cells. Immunohistochemistry in formalinfixed<br />
paraffin-embedded tissue demonstrated that the lymphoma cells marked with CD20, CD30, CD45 (focal), CD79a, BCL2<br />
(focal and weak), PAX5, Oct2, BOB. 1, IRF4/MUM1, EBV-LMP1, and TIA-1, but they did not mark with CD3, CD4, CD5, CD7, CD8,<br />
CD10, CD15, CD23, CD56, CD57, CD138, kappa and lambda immunoglobulin light chains, BCL6, βF1, granzyme B, or perforin.<br />
The aberrant TIA-1 expression was then confirmed by two different autostainers. EBV-encoded RNA (EBER) in situ hybridization<br />
was positive in the lymphoma cells. Gel electrophoresis for immunoglobulin heavy chain (IgH) and T-cell receptor (TCR) gene<br />
rearrangements revealed no definite clonal band, but fluorescence capillary electrophoresis demonstrated positive results for<br />
both IgH and TCR genes. Finally, we concluded this case as an EBV-positive DLBCL <strong>of</strong> the elderly with dual genotype<br />
(rearrangements <strong>of</strong> both IgH and TCR genes) and aberrant TIA-1 protein expression. © <strong>2010</strong> Springer-Verlag.<br />
No.1029<br />
Author (s) : Pongsai, P., Atamasirikul, K., Sungkanuparph, S.<br />
Title : The role <strong>of</strong> serum cryptococcal antigen screening for the early diagnosis <strong>of</strong> cryptococcosis in HIV-infected patients with<br />
different ranges <strong>of</strong> CD4 cell counts<br />
Source : Journal <strong>of</strong> Infection, Volume 60, Issue 6, June <strong>2010</strong>, Pages 474-477<br />
Document Type : Article<br />
Keywords : AIDS; Cryptococcosis; HIV; Screening; Serum cryptococcal antigen<br />
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<strong>Abstract</strong> : Objective: To determine the role <strong>of</strong> serum cryptococcal antigen (SCA) for the screening <strong>of</strong> cryptococcosis in HIV-infected<br />
patients with different ranges <strong>of</strong> CD4 cell counts. Methods: A retrospective cohort study was conducted in antiretroviral-naïve<br />
HIV-infected patients who had no symptom and had been screened with SCA in a tertiary-care hospital. Prevalence <strong>of</strong><br />
positive SCA at different ranges <strong>of</strong> CD4 cell counts, risk factor <strong>of</strong> positive SCA, and incidence <strong>of</strong> cryptococcosis during one-year<br />
follow-up period after negative SCA were determined. Results: There were 131 patients with a mean age <strong>of</strong> 38.5 years; 61.8%<br />
were male. Median (range) CD4 was 62 (3-590) cells/mm3. The overall prevalence <strong>of</strong> positive SCA was 9.2%. This prevalence<br />
in patients with CD4 < 100, 100-199, and ≥200 cells/mm3 were 12.9%, 3.6%, and 0%, respectively (P = 0.041). In<br />
multivariate analysis, CD4 < 100 cells/mm3 was associated with positive SCA (OR = 6.69; 95% CI, 1.03-23.56). Four (33.3%) <strong>of</strong><br />
12 patients with positive SCA had cryptococcosis whereas one (0.8%) <strong>of</strong> 119 patients with negative SCA developed meningitis<br />
at one-year follow-up. Conclusions: SCA screening has a substantial role for the early detection <strong>of</strong> cryptococcal infection in<br />
HIV-infected patients with low CD4 cell counts. Routine screening with SCA should be performed in patients with CD4 < 100<br />
cells/mm3. © <strong>2010</strong> The British Infection Society.<br />
No.1030<br />
Author (s) : Pongsiri, P., Auksornkitti, V., Theamboonlers, A., Luplertlop, N., Rianthavorn, P., Poovorawan, Y.<br />
Title : Entire genome characterization <strong>of</strong> Chikungunya virus from the 2008-2009 outbreaks in Thailand<br />
Source : Tropical Biomedicine, Volume 27, Issue 2, <strong>2010</strong>, Pages 167-176<br />
Document Type : Article<br />
Keywords : Alphavirus infection, article, Chikungunya alphavirus, epidemic, genetics, genotype, human, phylogeny, Thailand, virology, virus<br />
genome; Alphavirus Infections, Chikungunya virus, Disease Outbreaks, Genome, Viral, Genotype, Humans, Phylogeny, Thailand<br />
<strong>Abstract</strong> : The resurgence <strong>of</strong> Chikungunya virus (CHIKV) in the southern, northeastern and northern parts <strong>of</strong> Thailand, inflicting<br />
approximately 46,000 reported cases since October 2008 until December 2009, has raised public health concerns. In the<br />
present study, we characterized nearly complete genome sequences <strong>of</strong> four CHIKV isolates obtained from 2008 to 2009<br />
outbreaks in Thailand. Phylogenetic analysis was performed to determine the relationships <strong>of</strong> the study viruses with<br />
previously reported isolates. Results showed that 2008-2009 Thailand isolates belonged to the East, Central and South African<br />
genotype and were most closely related to isolates detected in Malaysia and Singapore in 2008. This was in contrast to<br />
isolates from all previous outbreaks in Thailand which were caused by an Asian genotype. We describe several novel<br />
mutations in Thailand isolates that warrants further investigation on characterization <strong>of</strong> CHIKV from different parts <strong>of</strong> the<br />
country to better understand the molecular epidemiology <strong>of</strong> Chikungunya fever outbreaks in Thailand.<br />
No.1031<br />
Author (s) : Pongsthorn, C., Ozawa, H., Aizawa, T., Kusakabe, T., Nakamura, T., Itoi, E.<br />
Title : Giant sacral schwannoma: A report <strong>of</strong> six cases<br />
Source : Upsala Journal <strong>of</strong> Medical Sciences, Volume 115, Issue 2, 12 May <strong>2010</strong>, Pages 146-152<br />
Document Type : Article<br />
Keywords : Pelvis; Sacrum; Schwannoma; Subtotal excision<br />
<strong>Abstract</strong> : Sacral and presacral schwannomas are <strong>of</strong>ten found incidentally, because they present with vague symptoms or symptomless.<br />
Schwannoma occurring in this area occasionally presents with enormous dimensions, known as a giant schwannoma. The<br />
tumor removal is a surgical challenge due to the difficult approach and abundant vascularity. The aim <strong>of</strong> this study is to<br />
review cases <strong>of</strong> giant sacral schwannomas focusing the surgical management and outcome. Six patients with sacral and<br />
presacral schwannoma were treated surgically. The patients included two males and four females, and the mean age was<br />
47.8 years. All patients experienced pain at the time <strong>of</strong> presentation. The tumors were classified as intraosseous type in one<br />
case, dumb-bell type in four cases, and retroperitoneal type in one case. The tumors were removed with a piecemeal<br />
subtotal excision in three patients, a partial excision in two patients, and enucleation in one patient. The surgeries were<br />
performed by the combination <strong>of</strong> an anterior and posterior approach in three patients, a posterior approach in two patients,<br />
and an anterior approach in one patient. The mean surgical time was 7.8 hrs, and the mean blood loss was 2572 g. The<br />
tumor recurred in one patient after the partial excision and was removed completely in a second surgery. No patient,<br />
including the patient who underwent the second surgery, presented with pain and obvious neurological deficit at the final<br />
follow-up. The surgical treatment <strong>of</strong> the giant sacral schwannoma with a piecemeal subtotal excision can achieve a good<br />
outcome, avoiding unnecessary neurological deficit. © <strong>2010</strong> Nordic Orthopedic Federation.<br />
377
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.1032<br />
Author (s) : Pongsumpun, P., Tang, I.-M.<br />
Title : Impact <strong>of</strong> cross-border migration on disease epidemics: Case <strong>of</strong> the P. falciparum and P. vivax malaria epidemic along the<br />
Thai-Myanmar border<br />
Source : Journal <strong>of</strong> Biological Systems, Volume 18, Issue 1, March <strong>2010</strong>, Pages 55-73<br />
Document Type : Article<br />
Keywords : Basic Reproduction Number; Burmese; Equilibrium States; Local Stability; Plasmodium vivax Malaria; Thai; Transmission<br />
Models<br />
<strong>Abstract</strong> : The transmission <strong>of</strong> Plasmodium falciparum and Plasmodium vivax malaria in a mixed population <strong>of</strong> Thais and migrant<br />
Burmese living along the Thai-Myanmar border is studied through a mathematical model. The population is separated into<br />
two groups: Thai and Burmese. Each population in turn is divided into susceptible, infected, recovered and in case <strong>of</strong> vivax<br />
infection, a dormant subclass. The model is then modified to allow for some <strong>of</strong> the Burmese (given as a fraction P) to be<br />
infectious when they enter into Thailand. The behaviour <strong>of</strong> the modified model is obtained using a standard dynamical<br />
analysis. A new basic reproduction number is obtained. Numerical simulations <strong>of</strong> the modified model show that when P ≠ 0<br />
and the same set <strong>of</strong> parameter values used in the initial model are used, the Thai population will be in the epidemic state. In<br />
other words, the repeated introduction <strong>of</strong> infectious Burmese (no matter how small <strong>of</strong> a number) will result in a malaria<br />
epidemic among the Thais irregardless <strong>of</strong> the public health practice undertaken by the Thai government. In the presence <strong>of</strong><br />
the infected Burmese, the Thai government would have to increase the facilitites to treat the people who are infected by the<br />
malaria. © <strong>2010</strong> World Scientific Publishing Company.<br />
No.1033<br />
Author (s) : Pongtanakul, B.; Narkbunnam, N.; Mahasandana, C.<br />
Title : Epitope mapping <strong>of</strong> factor VIII inhibitor antibodies in Thai hemophilia patients with inhibitors<br />
Source : HAEMOPHILIA, Volume: 16, Supplement: 4, Pages: 87-87, Published: JUL <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1034<br />
Author (s) : Pongtanakul, B.; Sangruchi, T.; Nunta-Aree, S.; Chawalparit, O.; Suntornpong, N.; Veerakul, G.<br />
Title : OUTCOME OF CHILDHOOD MEDULLOBLASTOMA IN THAILAND<br />
Source : NEURO-ONCOLOGY, Volume: 12, Issue: 6, Pages: II61-II61, Published: JUN <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1035<br />
Author (s) : Pongtippatee, P., Luppanakane, R., Thaweethamsewee, P., Kirirat, P., Weerachatyanukul, W., Withyachumnarnkul, B.<br />
Title : Delay <strong>of</strong> the egg activation process in the Black Tiger Shrimp Penaeus monodon by manipulation <strong>of</strong> magnesium levels in<br />
spawning water<br />
Source : Aquaculture Research, Volume 41, Issue 2, January <strong>2010</strong>, Pages 227-232<br />
Document Type : Article<br />
Keywords : Cortical rod; Egg activation; Magnesium effects; Penaeus monodon; Polyploidy<br />
<strong>Abstract</strong> : The aim <strong>of</strong> this study was to determine whether magnesium (Mg2+) in seawater is required for egg activation <strong>of</strong> the black tiger<br />
shrimp Penaeus monodon and whether manipulation <strong>of</strong> Mg2+ levels can be used to delay the process and thereby<br />
synchronize egg activation. Female P. monodon broodstock were allowed to spawn in artificial seawater containing Mg2+ at<br />
varying levels with respect to the normal (100%) level: 100%, 50%, 20% and 0%. Egg activation occurred normally at 100%<br />
Mg2+, incompletely at 50% and 20% Mg2+ levels and did not occur at all with 0% Mg2+. The fertilization rate with 100%<br />
Mg2+ was observed to be 83%, but fertilization failed to take place in all the other groups. The fertilization rate was restored<br />
from 0% to 76% following the 20% Mg2+ level treatment when Mg2+ levels returned to normal (100%) as soon as spawning<br />
was completed. This study suggests that the level <strong>of</strong> Mg2+ in seawater plays a vital role in P. monodon egg activation, and<br />
378
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
that commencement <strong>of</strong> this process could be delayed by manipulation <strong>of</strong> the Mg2+ level during and immediately after<br />
spawning. © 2009 Blackwell Publishing Ltd.<br />
No.1036<br />
Author (s) : Ponkham, P., Daduang, S., Kitimasak, W., Krittanai, C., Chokchaichamnankit, D., Srisomsap, C., Svasti, J., Kawamura, S., Araki, T.,<br />
Thammasirirak, S.<br />
Title : Complete amino acid sequence <strong>of</strong> three reptile lysozymes<br />
Source : Comparative Biochemistry and Physiology - C Toxicology and Pharmacology, Volume 151, Issue 1, January <strong>2010</strong>, Pages 75-83<br />
Document Type : Article<br />
Keywords : Amino acid sequence; Amino acid substitution; Amyda cartilaginea; C-type lysozyme; Complete amino acid<br />
sequence; Peptide mapping; Reptile lysozyme; S<strong>of</strong>t-shelled turtle lysozyme<br />
<strong>Abstract</strong> : To study the structure and function <strong>of</strong> reptile lysozymes, we have reported their purification, and in this study we have<br />
established the amino acid sequence <strong>of</strong> three egg white lysozymes in s<strong>of</strong>t-shelled turtle eggs (SSTL A and SSTL B from<br />
Trionyx sinensis, ASTL from Amyda cartilaginea) by using the rapid peptide mapping method. The established amino acid<br />
sequence <strong>of</strong> SSTL A, SSTL B, and ASTL showed substitutions <strong>of</strong> 43, 42, and 44 residues respectively when compared with the<br />
HEWL (hen egg white lysozyme) sequence. In these reptile lysozymes, SSTL A had one substitution compared with SSTL B<br />
(Gly126Asp) and had an N-terminal extra Gly and 11 substitutions compared with ASTL. SSTL B had an N-terminal extra Gly<br />
and 10 residues different from ASTL. The sequence <strong>of</strong> SSTL B was identical to s<strong>of</strong>t-shelled turtle lysozyme from STL (Trionyx<br />
sinensis japonicus). The Ile residue at position 93 <strong>of</strong> ASTL is the first report in all C-type lysozymes. Furthermore, amino acid<br />
substitutions (Phe34His, Arg45Tyr, Thr47Arg, and Arg114Tyr) were also found at subsites E and F when compared with HEWL.<br />
The time course using N-acetylglucosamine pentamer as a substrate exhibited a reduction <strong>of</strong> the rate constant <strong>of</strong> glycosidic<br />
cleavage and increase <strong>of</strong> binding free energy for subsites E and F, which proved the contribution for amino acids mentioned<br />
above for substrate binding at subsites E and F. Interestingly, the variable binding free energy values occurred on ASTL, may<br />
be contributed from substitutions at outside <strong>of</strong> subsites E and F. © 2009 Elsevier Inc. All rights reserved.<br />
No.1037<br />
Author (s) : Ponla, S., Auethavekiat, S., Phongsuphap, S.<br />
Title : Frame reconstruction by double motion estimation <strong>of</strong> affine parameters<br />
Source : ISCIT <strong>2010</strong> - <strong>2010</strong> 10th <strong>International</strong> Symposium on Communications and Information Technologies, <strong>2010</strong>, Article number<br />
5665136, Pages 1007-1012<br />
Document Type : Conference Paper<br />
Keywords :<br />
<strong>Abstract</strong> : In video frame rate conversion, unavailable frames are reconstructed from existing frames based on interpolated motion<br />
vectors. Due to an error in estimating motion vectors and an occlusion effect, it is possible that intensity information is not<br />
provided to every pixel in the reconstructed frame. Pixels whose intensity is missing become holes in the reconstructed<br />
frame. This paper proposes the reconstruction without distinct holes by double motion vector estimation. Motion is described<br />
by affine model instead <strong>of</strong> the conventional 2D translation model. The proposed method applies block based motion<br />
estimation. It is divided into 2 stages: frame reconstruction and hole filling. In the reconstruction stage, a new frame is<br />
reconstructed by moving blocks in previous frames according to their interpolated motion vectors. Holes in the newly<br />
reconstructed frame are then filled with the corresponding pixels in the subsequent frame. The proposed method searches<br />
for the corresponding pixels by looking for the region in the subsequent frames that are similar to the surrounding region <strong>of</strong><br />
each hole. The computation cost in the proposed method is low because the motion estimation is simple and applied only<br />
twice. It does not use any complex processes to fill holes. Experiments on three standard video sequences showed that our<br />
proposed method reconstructed frames in complex video sequence without noticeable holes. ©<strong>2010</strong> IEEE.<br />
No.1038<br />
Author (s) : Ponpuak, M., Davis, A.S., Roberts, E.A., Delgado, M.A., Dinkins, C., Zhao, Z., Virgin, H.W., Kyei, G.B., Johansen, T., Vergne, I.,<br />
Deretic, V.<br />
Title : Delivery <strong>of</strong> Cytosolic Components by Autophagic Adaptor Protein p62 Endows Autophagosomes with Unique Antimicrobial<br />
Properties<br />
Source : Immunity, Volume 32, Issue 3, March <strong>2010</strong>, Pages 329-341<br />
Document Type : Article<br />
379
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : MOLIMMUNO<br />
<strong>Abstract</strong> : Autophagy allows cells to self-digest portions <strong>of</strong> their own cytoplasm for a multitude <strong>of</strong> physiological purposes, including<br />
innate and adaptive immunity functions. In one <strong>of</strong> its innate immunity manifestations, autophagy, is known to contribute to<br />
the killing <strong>of</strong> intracellular microbes, including Mycobacterium tuberculosis, although the molecular mechanisms have been<br />
unclear. Here, we delineated sequential steps <strong>of</strong> the autophagic pathway necessary to control intracellular M. tuberculosis<br />
and found that in addition to autophagy initiation and maturation, an accessory autophagy-targeting molecule p62 (A170 or<br />
SQSTM1) was required for mycobactericidal activity. The p62 adaptor protein delivered specific ribosomal and bulk<br />
ubiquitinated cytosolic proteins to autolysosomes where they were proteolytically converted into products capable <strong>of</strong> killing<br />
M. tuberculosis. Thus, p62 brings cytosolic proteins to autolysosomes where they are processed from innocuous precursors<br />
into neo-antimicrobial peptides, explaining in part the unique bactericidal properties <strong>of</strong> autophagic organelles. © <strong>2010</strong> Elsevier<br />
Inc.<br />
No.1039<br />
Author (s) : Poomsawat, S., Buajeeb, W., Khovidhunkit, S., Punyasingh, J.<br />
Title : Alteration in the expression <strong>of</strong> cdk4 and cdk6 proteins in oral cancer and premalignant lesions<br />
Source : Journal <strong>of</strong> Oral Pathology and Medicine, Volume 39, Issue 10, November <strong>2010</strong>, Pages 793-799<br />
Document Type : Article<br />
Keywords : Cdk4; Cdk6; Dysplasia; Oral cancer; Oral leukoplakia<br />
<strong>Abstract</strong> : Background: Cdk4 and cdk6, key players in G1 phase, have been shown to play an important role in the development <strong>of</strong> oral<br />
squamous cell carcinoma (OSCC). This study investigated the expression <strong>of</strong> these two proteins in OSCC and premalignant<br />
lesions including oral leukoplakia (OL) with and without dysplasia and determined if alterations in the expression <strong>of</strong> these two<br />
proteins could be used as markers <strong>of</strong> malignant transformation.Methods: Expressions <strong>of</strong> cdk4 and cdk6 were evaluated in 61<br />
samples including OSCC, OL with and without dysplasia and normal oral mucosa using immunohistochemistry method.<br />
Nuclear staining <strong>of</strong> the keratinocytes was considered positive and the percentage <strong>of</strong> positive cells was calculated.Results:<br />
Expression <strong>of</strong> cdk4 was found in 11/15 (73.33%) OSCC, 13/14 (92.85%) OL with dysplasia, 13/20 (65%) OL without dysplasia<br />
and 3/12 (25%) normal mucosa. Expression <strong>of</strong> cdk6 was detected in 9/15 (60%) OSCC, 3/14 (21.43%) OL with dysplasia, 5/20<br />
(25%) OL without dysplasia and 1/12 (8.33%) normal mucosa. In cdk4 stained specimens, the frequency <strong>of</strong> positive cases and<br />
the percentage <strong>of</strong> positive cells in normal mucosa was significantly lower than OL with dysplasia and OSCC. For cdk6 staining,<br />
the prevalence <strong>of</strong> positive cases and the percentage <strong>of</strong> positive cells in normal mucosa were significantly lower than<br />
OSCC.Conclusions: Overexpressions <strong>of</strong> cdk4 and cdk6 were observed in OSCC, indicating that these two proteins play a crucial<br />
role in OSCC. The aberrant expression <strong>of</strong> cdk4 was found in OL with dysplasia, suggesting that cdk4 may be involved in the<br />
early event <strong>of</strong> carcinogenesis. © <strong>2010</strong> John Wiley & Sons A/S.<br />
No.1040<br />
Author (s) : Poomthavorn, P., Isaradisaikul, B., Chuansumrit, A., Khlairit, P., Sriphrapradang, A., Mahachoklertwattana, P.<br />
Title : High prevalence <strong>of</strong> "biochemical" adrenal insufficiency in thalassemics: Is it a matter <strong>of</strong> different testings or decreased cortisol<br />
binding globulin?<br />
Source : Journal <strong>of</strong> Clinical Endocrinology and Metabolism, Volume 95, Issue 10, October <strong>2010</strong>, Pages 4609-4615<br />
Document Type : Article<br />
Keywords : alanine aminotransferase, aspartate aminotransferase, corticotropin, gamma glutamyltransferase, globulin, hydrocortisone,<br />
hydrocortisone binding globulin, insulin, tetracosactide, unclassified drug; adolescent, adrenal function, adrenal<br />
insufficiency, article, beta thalassemia, blood sampling, blood transfusion, calculated free cortisol, clinical<br />
assessment, controlled study, cross-sectional study, disease marker, endocrine function test, female, free cortisol<br />
index, human, insulin tolerance test, major clinical study, male, prevalence, priority journal, protein blood level, screening<br />
test, Thailand, thalassemia, thalassemia major; Adolescent, Adrenal Insufficiency, Biological Markers, Carrier<br />
Proteins, Child, Child, Preschool, Cosyntropin, Cross-Sectional Studies, Diagnosis, Differential, Down-Regulation, Humans,<br />
Hydrocortisone, Pituitary-Adrenal Function Tests, Prevalence, Thalassemia<br />
<strong>Abstract</strong> : Context: High prevalence <strong>of</strong> "biochemical" adrenal insufficiency (AI) in thalassemics has been reported. However, "clinical" AI is<br />
rare. Aim: The aim was to determine whether cortisol binding globulin (CBG) or tests used in assessing adrenal function<br />
contributed to the abnormally high prevalence <strong>of</strong> biochemical AI. Setting: The study was conducted at Ramathibodi Hospital,<br />
<strong>Mahidol</strong> <strong>University</strong>, Bangkok, Thailand. Participants: Participants included 56 children and adolescents with thalassemia and 44<br />
controls. Main Outcome Measures: Serum CBG and adrenal function test results assessed by 1 μg cosyntropin test and insulin<br />
380
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
tolerance test (ITT) were measured. Free cortisol index (FCI) calculated by total cortisol (TC)/CBG and calculated free cortisol<br />
(cFC) were determined. Results: Mean (SD) CBG levels were comparable between patients and controls [45.2 (11.0) vs. 47.0<br />
(8.6) mg/liter]. Peak TC, FCI, and cFC after cosyntropin test were lower in thalassemics [TC, 15.2 (4.0) vs. 18.9 (3.1) μg/dl; FCI,<br />
3.4 (0.8) vs. 4.2 (1.2) μg/mg, P
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Human face recognition by Euclidean distance and neural network<br />
Source : Proceedings <strong>of</strong> SPIE - The <strong>International</strong> Society for Optical Engineering, Volume 7546, <strong>2010</strong>, Article number 754603<br />
Document Type : Conference Paper<br />
Keywords : Euclidean distance; Face extraction; Face recognition; Neural network<br />
<strong>Abstract</strong> : The idea <strong>of</strong> this project development is to improve the concept <strong>of</strong> human face recognition that has been studied in order to<br />
apply it for a more precise and effective recognition <strong>of</strong> human faces, and <strong>of</strong>fered an alternative to agencies with respect to<br />
their access-departure control system. To accomplish this, a technique <strong>of</strong> calculation <strong>of</strong> distances between face features,<br />
including efficient face recognition though a neural network, is used. The system uses a technique <strong>of</strong> image processing<br />
consisting <strong>of</strong> 3 major processes: 1) preprocessing or preparation <strong>of</strong> images, 2) feature extraction from images <strong>of</strong> eyes, ears,<br />
nose and mouth, used for a calculation <strong>of</strong> Euclidean distances between each organ; and 3) face recognition using a neural<br />
network method. Based on the experimental results from reading image <strong>of</strong> a total <strong>of</strong> 200 images from 100 human faces, the<br />
system can correctly recognize 96 % with average access time <strong>of</strong> 3.304 sec per image. © <strong>2010</strong> Copyright SPIE - The<br />
<strong>International</strong> Society for Optical Engineering.<br />
No.1045<br />
Author (s) : Pornpanomchai, C., Jurangboon, K., Jantarasee, K.<br />
Title : Instant c<strong>of</strong>fee classification by electronic noses<br />
Source : ICMEE <strong>2010</strong> - <strong>2010</strong> 2nd <strong>International</strong> Conference on Mechanical and Electronics Engineering, Proceedings, Volume 1, <strong>2010</strong>,<br />
Article number 5558605, Pages V110-V113<br />
Document Type : Conference Paper<br />
Keywords : C<strong>of</strong>fee identification; Electronic noses; Neural network<br />
<strong>Abstract</strong> : Normally, an electronic nose project uses two researches areas which are hardware for developing sensors to detect<br />
substance smell and s<strong>of</strong>tware using pattern matching theorem for recognizing substance. For this research, the operation<br />
begins with sensors hit the c<strong>of</strong>fee smell. The result is converted from analog to digital representation. An artificial intelligence<br />
is a tool <strong>of</strong> a thinking system which can create knowledge as if a human does. The objective <strong>of</strong> this research is to classify<br />
instant c<strong>of</strong>fee by using electronic noses. We used eight types <strong>of</strong> c<strong>of</strong>fee in Thailand market for this project which are 1)<br />
Moccona-select, 2) Moccona-royal gold, 3) Nescafe redcup, 4) Nescafe gold, 5) Khao Shong brown, 6) Khao Shong red, 7)<br />
Oem-Big C and 8) Superclass. We compared four structures <strong>of</strong> neural network to classify the c<strong>of</strong>fee data. The precision <strong>of</strong><br />
correctness is equal to 65.63 for a neural network structure as 7 input-layer nodes, 14 hidden-layerl nodes, 48 hidden-layer2<br />
nodes and 8 output-layer nodes. © <strong>2010</strong> IEEE.<br />
No.1046<br />
Author (s) : Pornpanomchai, C., Panyasrivarom, P., Pisitviroj, N., Prutkraiwat, P.<br />
Title : Thai handwritten character recognition by Euclidean distance<br />
Source : Proceedings <strong>of</strong> SPIE - The <strong>International</strong> Society for Optical Engineering, Volume 7546, <strong>2010</strong>, Article number 75460A<br />
Document Type : Conference Paper<br />
Keywords : Character recognition; Euclidean distance; Thai handwritten character<br />
<strong>Abstract</strong> : This research applied the Euclidean distance technique to generate a system <strong>of</strong> Thai handwritten character recognition. The<br />
system consists <strong>of</strong> four main components which include: 1) Image Acquisition, 2) Image Pre-processing, 3) Recognition, and 4)<br />
Display Result. All training and testing handwritten characters in this research used all Thai native people to write them for<br />
avoiding invalid shape <strong>of</strong> Thai character. The character images fed to the training part totaling 3,513 characters. Out <strong>of</strong> 878<br />
Thai handwritten characters tested, it was found that the system could recognize (accept) 716 characters or 81.55%, while<br />
rejecting 61 characters or 6.95% and misrecognizing 101 characters or 11.50%. We tested the system with 50 Japanese<br />
handwritten characters and 25 invalid Thai handwritten character shape, it was found that the system could reject 47<br />
characters or 62.67% while misrecognizing 28 characters or 37.33%. © <strong>2010</strong> Copyright SPIE - The <strong>International</strong> Society for<br />
Optical Engineering.<br />
No.1047<br />
Author (s) : Pornpanomchai, C., Phaisitkulwiwat, A.<br />
Title : Fingerprint recognition by euclidean distance<br />
Source : 2nd <strong>International</strong> Conference on Computer and Network Technology, ICCNT <strong>2010</strong>, <strong>2010</strong>, Article number 5474459, Pages 437-<br />
441<br />
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Document Type : Conference Paper<br />
Keywords : Euclidean distance; Fingerprint recognition<br />
<strong>Abstract</strong> : This research proposed the fingerprint recognition by euclidean distance method. the system uses a technique <strong>of</strong> image<br />
processing, which consists <strong>of</strong> 3 major components, which are: 1) preprocessing component, the module that reduces the<br />
noise <strong>of</strong> the original images and adjusts the sharpness <strong>of</strong> the lined pattern that is the components <strong>of</strong> the fingerprint, 2)<br />
feature extraction component, the module that defines the position <strong>of</strong> the core point used as a reference point and finds out<br />
the position <strong>of</strong> the bifurcation points, and 3) fingerprint recognition component, the module to compare the shape context <strong>of</strong><br />
training and testing data sets. based on the experimental results, the system has acceptable accuracy with average access<br />
time <strong>of</strong> around 19.68 seconds per image. © <strong>2010</strong> IEEE.<br />
No.1048<br />
Author (s) : Pornpanomchai, C., Sakunreraratsame, P., Wongsasirinart, R., Youngtavichavhart, N.<br />
Title : Herb flower recognition system (HFRS)<br />
Source : ICEIE <strong>2010</strong> - <strong>2010</strong> <strong>International</strong> Conference on Electronics and Information Engineering, Proceedings, Volume 1, <strong>2010</strong>, Article<br />
number 5559906, Pages V1123-V1127<br />
Document Type : Conference Paper<br />
Keywords : Flower features extraction; Herb flower recognition; Minimum distance method<br />
<strong>Abstract</strong> : The objective <strong>of</strong> this research is to build an automatic method for recognizing a Thai herb flower based on the Minimum<br />
Distance Method. The herb flower images, acquired from a digital camera, are taken in the real environment. We use the<br />
characteristics <strong>of</strong> herb flowers to design our classification algorithms, which consist <strong>of</strong> the average red, green and blue (RGB)<br />
colors, the herb flower size and the edge <strong>of</strong> petals feature. The experiments are conducted on more than 380 pictures from<br />
16 species <strong>of</strong> herb flowers. The training data set is around 220 pictures. We test the system by using 110 pictures for a training<br />
data set and 50 pictures for an un-training data set. The precision rates <strong>of</strong> the recognition system are 98.18 percent and 94<br />
percent, respectively. The average access time is 0.87 seconds per image. © <strong>2010</strong> IEEE.<br />
No.1049<br />
Author (s) : Pornpanomchai, C., Wiranurak, B., Weerawut, W., Kawrungruang, L.<br />
Title : Operation <strong>of</strong> a radio-controlled car using face images<br />
Source : ICMEE <strong>2010</strong> - <strong>2010</strong> 2nd <strong>International</strong> Conference on Mechanical and Electronics Engineering, Proceedings, Volume 1, <strong>2010</strong>,<br />
Article number 5558586, Pages V1108-V1112<br />
Document Type : Conference Paper<br />
Keywords : Face commands; Face image; Radio-controlled car (RC car)<br />
<strong>Abstract</strong> : The objective <strong>of</strong> this research is to operate a radio-controlled car (RC car) by using facial images. The research was conducted<br />
by taking a face image by a webcam and sending the image to a computer machine, and then the computer converted the<br />
face image into digital signal. After that, the digital signal was converted into a radio wave command. Finally, an RC car<br />
operated based on the radio wave commands. The experiment was conducted on the RC car with nine facial commands,<br />
which were: 1) Stop, 2) Forward, 3) Reverse, 4) Turn Left, 5) Turn Right, 6) Forward & Turn Left, 7) Reverse & Turn Left, 8)<br />
Forward & Turn Right, and 9) Reverse & Turn Right. The experimental precisions are 84.11%, 10.33% and 5.56% for match,<br />
mismatch and reject, respectively in the sufficiently lighted environment and 39.11%, 18.00% and 42.89% for match,<br />
mismatch and reject, respectively in an insufficiently lighted environment. © <strong>2010</strong> IEEE.<br />
No.1050<br />
Author (s) : Pornpanomchai, C., Wongkorsub, J., Pornaudomdaj, T., Vessawasdi, P.<br />
Title : Buddhist Amulet Recognition System (BARS)<br />
Source : 2nd <strong>International</strong> Conference on Computer and Network Technology, ICCNT <strong>2010</strong>, <strong>2010</strong>, Article number 5474450, Pages 495-<br />
499<br />
Document Type : Conference Paper<br />
Keywords : Buddhist Amulet Recognition; Image processing; Template matching<br />
<strong>Abstract</strong> : There are many kinds <strong>of</strong> Buddha amulets, which are difficult for people who want to recognize all <strong>of</strong> them. The objective <strong>of</strong><br />
this research is to build the computer system that can recognize some Thai Buddhist amulets. Our system is called "Buddhist<br />
Amulet Recognition System or BARS". It consists <strong>of</strong> 4 main components; 1) image acquisition, 2) image preprocessing, 3) image<br />
recognition, and 4) display result. In image acquisition component, the Buddhist amulet picture is taken by a digital camera<br />
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with a white paper for the picture background. In image preprocessing component, the Buddhist amulet image is enhanced<br />
for improving a recognition precision rate in the next stage. In recognition component, a template matching technique is<br />
applied to recognize the Buddhist amulet image. In display result component, a graphic user interface (GUI) is created for<br />
displaying the recognition results. We have collected 52 kinds <strong>of</strong> Buddhist amulets with the total <strong>of</strong> 318 images for the<br />
training data set. We determine correlation value equal to 500 and this program precision equal to 80 percent and take 0.76<br />
milliseconds per image. © <strong>2010</strong> IEEE.<br />
No.1051<br />
Author (s) : Pornratanarangsi, S., Boonlert, S., Duangprateep, A., Wiratpintu, P., Waree, W., Tresukosol, D., Panchavinnin, P.<br />
Title : The effectiveness <strong>of</strong> "Siriraj Leg Lock" brace on back pain after percutaneous coronary intervention: PCI.<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand = Chotmaihet thangphaet, Volume 93 Suppl 1, January <strong>2010</strong>, Pages S35-42<br />
Document Type : Article<br />
Keywords : adult, aged, angiocardiography, article, backache, bed rest, brace, clinical trial, controlled clinical trial, controlled<br />
study, female, hematoma, human, male, middle aged, nursing, pain assessment, patient satisfaction, postoperative<br />
care, randomized controlled trial, transluminal coronary angioplasty, treatment outcome; Adult, Aged, Aged, 80 and<br />
over, Angioplasty, Transluminal, Percutaneous Coronary, Back Pain, Bed Rest, Braces, Coronary<br />
Angiography, Female, Hematoma, Humans, Male, Middle Aged, Pain Measurement, Patient Satisfaction, Postoperative<br />
Care, Treatment Outcome<br />
<strong>Abstract</strong> : BACKGROUND: The most common problem experienced by patients after Percutaneous Coronary Intervention (PCI) is back<br />
pain. After the procedure, patients are restricted to complete bed rest with no hip flexion for up to 10 hours, causing much<br />
discomfort, especially back pain. In some patients, anxiety arises due to limited movement, with the belief that movement<br />
might cause re-bleeding from the wound. To alleviate these problems, the "Siriraj Leg Lock" brace or SLL was initiated to<br />
confine the hip while allowing patients to freely move other parts <strong>of</strong> their body without complicating the wound. Hence,<br />
anxiety is relieved and mobility can lower the chance <strong>of</strong> getting back pain with more comfort. OBJECTIVE: The aims <strong>of</strong> this<br />
experimental study were to investigate the effect and satisfaction <strong>of</strong> SLL on back pain in post-PCI patients, and to compare<br />
bleeding and/or hematoma occurrences at the site <strong>of</strong> incision between experimental and control groups. MATERIAL AND<br />
METHOD: The randomized controlled trial (RCT) was conducted in 100 patients who underwent coronary angioplasty and/or<br />
stent placement interventions and received post procedural care at the intermediate cardiac care ward, Her Majesty Cardiac<br />
Center from December 2006 to February 2007. The control group (49 patients) was to get standard care after the intervention,<br />
whereas the experimental group (51 patients) was fitted with the SLL device to allow free mobility right after the procedure.<br />
RESULTS: Lower maximum back pain scores and mean back pain scores in the experimental group than in the control group,<br />
with statistical significance (p < 0.001). Back pain score reduced from the day <strong>of</strong> admission in the experimental group after<br />
applying SLL, but increased in the control group. The occurrence <strong>of</strong> hematoma in both groups was not significantly different<br />
(p = 0.114). The experimental group with the SLL could freely change positions from upright to decubitus without any effects<br />
to the wound, feeling more comfortable and experiencing less back pain. Patients' satisfaction towards the SLL from 1 to 5<br />
scale was 4.3. The satisfaction was evident especially in patients who had previous PCI experience, and desired to ask for SLL<br />
application if a future procedure to be needed. CONCLUSION: Using SLL after sheath removal post PCI allows the patient to<br />
freely change position without any effects to the wound and reduces back pain with more patients' satisfaction.<br />
No.1052<br />
Author (s) : Porntadavity, S., Permpongpaiboon, T., Sukketsiri, W.<br />
Title : Human paraoxonase 2<br />
Source : EXCLI Journal, Volume 9, <strong>2010</strong>, Pages 159-172<br />
Document Type : Review<br />
Keywords : Antioxidant; Atherosclerosis; Oxidative stress; Paraoxonase 2<br />
<strong>Abstract</strong> : Human paraoxonase 2 (PON2), which is a member <strong>of</strong> the paraoxonase family, possesses unique properties that distinguish it<br />
from PON1 and PON3. PON2 is ubiquitously expressed in many different tissue types and is highly expressed in the vital<br />
organs, such as the heart and lungs. Early research revealed that PON2 is exclusively intracellularly found, wherein it functions<br />
as an anti-oxidative protein by reducing intracellular and local oxidative stress. Studies in the last five years have<br />
demonstrated that PON2 protects against atherosclerosis by preventing low-density lipoprotein (LDL) oxidation, reversing the<br />
oxidation <strong>of</strong> mildly oxidised LDL, inhibiting monocyte chemotaxis, and increasing cholesterol efflux. Recently, emerging<br />
evidence has proposed that PON2 is an anti-atherosclerotic and may be associated with cardiovascular disease (CVD). The<br />
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number <strong>of</strong> investigations concerning the relationship between two common PON2 polymorphisms and CVD among different<br />
ethnic groups and regions is rapidly growing. Here, we briefly review the developments in PON2 research by focusing on past<br />
and recent findings.<br />
No.1053<br />
Author (s) : Pothavorn, P., Kitdamrongsont, K., Swangpol, S., Wongniam, S., Atawongsa, K., Savasti, J., Somana, J.<br />
Title : Sap phytochemical compositions <strong>of</strong> some bananas in Thailand<br />
Source : Journal <strong>of</strong> Agricultural and Food Chemistry, Volume 58, Issue 15, 11 August <strong>2010</strong>, Pages 8782-8787<br />
Document Type : Article<br />
Keywords : Banana sap; flavonoids; LC-ESI-MS; Musa; polyphenols<br />
<strong>Abstract</strong> : Banana sap has some special properties relating to various phenomena such as browning <strong>of</strong> fruits after harvesting, permanent<br />
staining <strong>of</strong> cloth and fibers, and antioxidant and antibleeding properties. Analysis <strong>of</strong> banana sap using high-performance liquid<br />
chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS) indicated the presence <strong>of</strong> phenolic and aromatic<br />
amino compounds <strong>of</strong> interest due to their special properties. With the online positive electrospray ionization mode (ESI), the<br />
possible structures <strong>of</strong> specific compounds were determined from the fragmentation patterns <strong>of</strong> each particular ion appearing<br />
in the mass spectra. The major compounds revealed from the sap <strong>of</strong> banana accessions, namely, Musa balbisiana, Musa<br />
laterita, Musa ornata, and Musa acuminata, and some cultivars were apigenin glycosides, myricetin glycoside, myricetin-3-Orutinoside,<br />
naringenin glycosides, kaempferol-3-O-rutinoside, quercetin-3-O-rutinoside, dopamine, and N-acetylserotonin. The<br />
results indicated that there was a variety <strong>of</strong> phenolic and aromatic amino contents in many banana species. These<br />
compounds were reported to relate with biological activities. Moreover, the identities <strong>of</strong> these phytochemical compositions<br />
may be used as markers for banana diet, the assessment <strong>of</strong> physiochemical status, or the classification <strong>of</strong> banana clones. ©<br />
<strong>2010</strong> American Chemical Society.<br />
No.1054<br />
Author (s) : Pothikasikorn, J., Boonplueang, R., Suebsaeng, C., Khaengraeng, R., Chareonviriyaphap, T.<br />
Title : Feeding response <strong>of</strong> Aedes aegypti and Anopheles dirus (Diptera: Culicidae) using out-<strong>of</strong>-date human blood in a membrane<br />
feeding apparatus<br />
Source : Journal <strong>of</strong> Vector Ecology, Volume 35, Issue 1, June <strong>2010</strong>, Pages 149-155<br />
Document Type : Article<br />
Keywords : Aedes aegypti; Anopheles dirus; Artificial feeding; Colonization; Out-<strong>of</strong>-date blood; Thailand<br />
<strong>Abstract</strong> : The colonization <strong>of</strong> Aedes aegypti and Anopheles dirus was performed using out-<strong>of</strong>-date human blood from a blood bank as<br />
a nutritional supply dispensed from a common artificial feeder. Preserved human blood was collected and used for feeding<br />
on days 5, 15, and 25 after date <strong>of</strong> expiration and dispensed from a common artificial feeder to rear the mosquitoes. Ae.<br />
aegypti had a feeding rate <strong>of</strong> 78.7, 62, and 18% at the respective intervals while An. dirus had a rate <strong>of</strong> 80, 56.8, and 7.3% on<br />
the same respective days. Direct feeding on live hamsters resulted in a rate <strong>of</strong> 96 and 90% for Ae. aegypti and An. dirus,<br />
respectively. Although egg production rates decreased from the day 5 feeding to the day 25 feeding, all <strong>of</strong> the<br />
developmental stages resulting from An. dirus fed at day 5 and 15 showed insignificant differences when compared with<br />
direct feeding on the blood <strong>of</strong> a hamster.<br />
No.1055<br />
Author (s) : Pothitirat, W., Chomnawang, M.T., Gritsanapan, W.<br />
Title : Anti-acne-inducing bacterial activity <strong>of</strong> mangosteen fruit rind extracts<br />
Source : Medical Principles and Practice, Volume 19, Issue 4, May <strong>2010</strong>, Pages 281-286<br />
Document Type : Article<br />
Keywords : Acne vulgaris; Antiacne; Mangosteen; Mangostin; Propionibacterium acnes; Staphylococcus epidermidis<br />
<strong>Abstract</strong> : Objective: The aims <strong>of</strong> this study were to determine the most effective solvent extract <strong>of</strong> mangosteen, anti-acne- inducing<br />
bacterial activity and the amount <strong>of</strong> α-mangostin, a major active component in each mangosteen fruit rind extract, using<br />
high-performance liquid chromatography (HPLC). Materials and Methods: The fruit rinds <strong>of</strong> mangosteen were extracted with<br />
hexane, dichloromethane, ethanol and water. The extracts were tested for antibacterial activity against bacteria that induce<br />
acne, including Propionibacterium acnes and Staphylococcus epidermidis. Thin-layer chromatographic autobiography against<br />
these bacteria was also performed for each extract, while the α-mangostin content was analyzed using a validated HPLC<br />
method. Results: The dichloromethane extract exhibited the strongest antibacterial effect with minimum inhibitory<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
concentration values for both bacterial species at 3.91 μg/ml, while the minimum bactericidal concentration values against P.<br />
acnes and S. epidermidis were 3.91 and 15.63 μg/ml, respectively. Thin-layer chromatographic autobiography indicated that<br />
α-mangostin was present in all extracts, except the water extract, and is a major active component against both P. acnes and<br />
S. epidermidis. Using HPLC, the dichloromethane extract yielded the highest content (46.21% w/w) <strong>of</strong> α-mangostin followed<br />
by the ethanol extract (18.03% w/w), the hexane extract (17.21% w/w) and the water extract (0.54% w/w). Conclusions:<br />
Dichloromethane extract exhibited the strongest anti-acne-inducing bacterial effect and this extract yielded the highest<br />
amount <strong>of</strong> α-mangostin. © <strong>2010</strong> S. Karger AG.<br />
No.1056<br />
Author (s) : Pothitirat, W., Chomnawang, M.T., Supabphol, R., Gritsanapan, W.<br />
Title : Free radical scavenging and anti-acne activities <strong>of</strong> mangosteen fruit rind extracts prepared by different extraction methods<br />
Source : Pharmaceutical Biology, Volume 48, Issue 2, February <strong>2010</strong>, Pages 182-186<br />
Document Type : Article<br />
Keywords : Acne vulgaris; Anti-acne; Free radical scavenging activity; Garcinia mangostana; Mangostin; Tannin<br />
<strong>Abstract</strong> : The ethanol extracts <strong>of</strong> mangosteen fruit rinds prepared by several extraction methods were examined for their contents <strong>of</strong><br />
bioactive compounds, DPPH-scavenging activity, and anti-acne producing bacteria against Propionibacterium acnes and<br />
Staphylococcus epidermidis. The dried powder <strong>of</strong> the fruit rind was extracted with 95% ethanol by maceration, percolation,<br />
Soxhlet extraction, ultrasonic extraction, and extraction using a magnetic stirrer. Soxhlet extraction promoted the maximum<br />
contents <strong>of</strong> crude extract (26.60% dry weight) and α-mangostin (13.51%, w/w <strong>of</strong> crude extract), and also gave the highest<br />
anti-acne activity with MIC 7.81 and 15.63μg/mL and MBC 15.53 and 31.25μg/mL against P. acnes and S. epidermidis,<br />
respectively. Ethanol 70% and 50% (v/v) were also compared in Soxhlet extraction. Ethanol 50% promoted the extract with<br />
maximum amounts <strong>of</strong> total phenolic compounds (26.96g gallic acid equivalents/100g extract) and total tannins (46.83g tannic<br />
acid equivalents/100g extract), and also exhibited the most effective DPPH-scavenging activity (EC50 12.84μg/mL). Considering<br />
various factors involved in the process, Soxhlet extraction carried a low cost in terms <strong>of</strong> reagents and extraction time. It<br />
appears to be the recommended extraction method for mangosteen fruit rind. Ethanol 50% should be the appropriate<br />
solvent for extracting free radical-scavenging components, phenolic compounds, and tannins, while 95% ethanol is<br />
recommended for extraction <strong>of</strong> α-mangostin, a major anti-acne component from this plant. © <strong>2010</strong> Informa UK Ltd.<br />
No.1057<br />
Author (s) : Pothitirat, W.; Gritsanapan, W.<br />
Title : Assessment <strong>of</strong> the phenolic content and free radical scavenging capacity <strong>of</strong> extracts obtained from the pericarp <strong>of</strong> Garcinia<br />
mangostana L.<br />
Source : PLANTA MEDICA, Volume: 76, Issue: 12, Pages: 1189-1189, Published: AUG <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1058<br />
Author (s) : Pothitirat, W.; Pluemlamai, J.; Satniyom, S.; Leelamanitaya, W.; Gritsanapan, W.<br />
Title : Development <strong>of</strong> whitening skin lotion from selected medicinal plants and its antityrosinase activity<br />
Source : PLANTA MEDICA, Volume: 76, Issue: 12, Pages: 1236-1236, Published: AUG <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1059<br />
Author (s) : Poungpair, O., Pootong, A., Maneewatch, S., Srimanote, P., Tongtawe, P., Songserm, T., Tapchaisri, P., Chaicumpa, W.<br />
Title : A human single chain transbody specific to matrix protein (M1) interferes with the replication <strong>of</strong> influenza a virus<br />
Source : Bioconjugate Chemistry, Volume 21, Issue 7, 21 July <strong>2010</strong>, Pages 1134-1141<br />
Document Type : Article<br />
Keywords : amino acid, antivirus agent, bacterial vector, DNA, hybrid protein, matrix protein, penetratin, penetratin single chain fragment<br />
variable antibody, single chain fragment variable antibody, unclassified drug; animal cell, antigen binding, article, cell<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
membrane, cell strain, confocal microscopy, controlled study, cytotoxicity, DNA template, Drosophila, gene<br />
sequence, immun<strong>of</strong>luorescence, influenza, Influenza virus A, life cycle, mammal cell, nonhuman, nucleotide<br />
sequence, phagemid, polymerase chain reaction, protein domain, virus replication; Antibody Specificity, Carrier Proteins, Cell<br />
Line, Humans, Influenza A virus, Single-Chain Antibodies, Viral Matrix Proteins, Virus Replication; Animalia, Influenza A<br />
virus, Mammalia, Orthomyxoviridae<br />
<strong>Abstract</strong> : A cell penetrating format <strong>of</strong> human single chain antibody (HuScFv) specific to matrix protein (M1) <strong>of</strong> influenza A virus was<br />
produced by molecular linking <strong>of</strong> the gene sequence encoding the HuScFv (huscfv) to a protein transduction domain, i.e.,<br />
penetratin (PEN) <strong>of</strong> the Drosophila homeodomain. DNA <strong>of</strong> a recombinant phagemid vector carrying the huscfv was used as a<br />
platform template in a three-step PCR for generating a nucleotide sequence encoding a 16 amino acid PEN peptide. The PEN-<br />
HuScFv had negligible cytotoxicity on living MDCK cells. They were readily translocated across the cell membrane and bound<br />
to native M1 in the A/H5N1-infected cells as revealed by immun<strong>of</strong>luorescent confocal microscopy. The PEN-HuScFv, when<br />
used to treat the influenza virus infected cells, reduced the number <strong>of</strong> viruses released from the cells. In conclusion, the cell<br />
penetrating M1-specific HuScFv, a transbody, produced in this study affected the influenza A virus life cycle in living<br />
mammalian cells. While the molecular mechanisms <strong>of</strong> the PEN-HuScFv need more investigation, the reagent warrants further<br />
testing in animals before developing it into a human immunotherapeutic anti-influenza formula. © <strong>2010</strong> American Chemical<br />
Society.<br />
No.1060<br />
Author (s) : Pourrut, X., Nkoghé, D., Souris, M., Paupy, C., Paweska, J., Padilla, C., Moussavou, G., Leroy, E.M.<br />
Title : Rift valley fever virus seroprevalence in human rural populations <strong>of</strong> Gabon<br />
Source : PLoS Neglected Tropical Diseases, Volume 4, Issue 7, July <strong>2010</strong>, Article number e763<br />
Document Type : Article<br />
Keywords : immunoglobulin G, virus antibody; adolescent, adult, aged, agricultural worker, article, blood sampling, controlled<br />
study, demography, ecosystem, female, forest, Gabon, human, lake, major clinical study, male, mosquito, Rift Valley fever<br />
bunyavirus, risk factor, rural population, savanna, seroprevalence, virus<br />
isolation, animal, blood, epidemiology, immunology, middle aged, Rift Valley fever; Adolescent, Adult, Aged, Aged, 80 and<br />
over, Animals, Antibodies, Viral, Female, Gabon, Humans, Immunoglobulin G, Male, Middle Aged, Rift Valley Fever, Rift Valley<br />
fever virus, Rural Population, Seroepidemiologic Studies, Young Adult<br />
<strong>Abstract</strong> : Background: Rift Valley fever (RVF) is a mosquito-borne viral zoonosis caused by a phlebovirus and transmitted by Aedes<br />
mosquitoes. Humans can also be infected through direct contact with blood (aerosols) or tissues (placenta, stillborn) <strong>of</strong><br />
infected animals. Although severe clinical cases can be observed, infection with RVF virus (RVFV) in humans is, in most cases,<br />
asymptomatic or causes a febrile illness without serious symptoms. In small ruminants RVFV mainly causes abortion and<br />
neonatal death. The distribution <strong>of</strong> RVFV has been well documented in many African countries, particularly in the north<br />
(Egypt, Sudan), east (Kenya, Tanzania, Somalia), west (Senegal, Mauritania) and south (South Africa), but also in the Indian<br />
Ocean (Madagascar, Mayotte) and the Arabian Peninsula. In contrast, the prevalence <strong>of</strong> RVFV has rarely been investigated in<br />
central African countries. Methodology/Principal Findings: We therefore conducted a large serological survey <strong>of</strong> rural<br />
populations in Gabon, involving 4,323 individuals from 212 randomly selected villages (10.3% <strong>of</strong> all Gabonese villages). RVFVspecific<br />
IgG was found in a total <strong>of</strong> 145 individuals (3.3%) suggesting the wide circulation <strong>of</strong> Rift Valley fever virus in Gabon.<br />
The seroprevalence was significantly higher in the lakes region than in forest and savannas zones, with respective rates <strong>of</strong><br />
8.3%, 2.9% and 2.2%. In the lakes region, RVFV-specific IgG was significantly more prevalent in males than in females<br />
(respectively 12.8% and 3.8%) and the seroprevalence increased gradually with age in males but not in females.<br />
Conclusions/Significance: Although RVFV was suggested to circulate at a relatively high level in Gabon, no outbreaks or even<br />
isolated cases have been documented in the country. The higher prevalence in the lakes region is likely to be driven by<br />
specific ecologic conditions favorable to certain mosquito vector species. Males may be more at risk <strong>of</strong> infection than females<br />
because they spend more time farming and hunting outside the villages, where they may be more exposed to mosquito bites<br />
and infected animals. Further investigations are needed to determine the putative sylvan cycle <strong>of</strong> RVFV, including the<br />
mosquito species and the reservoir role <strong>of</strong> wild animals in the viral maintenance cycle. © <strong>2010</strong> Pourrut et al.<br />
No.1061<br />
Author (s) : Prabhasawat, P., Leelaporn, A., Tesavibul, N., Uiprasertkul, M., Chirapapaisan, C.<br />
Title : Molecular identification by 16S rDNA sequencing using excised corneal tissues: A useful diagnostic tool for refractory keratitis<br />
Source : Japanese Journal <strong>of</strong> Ophthalmology, Volume 54, Issue 1, January <strong>2010</strong>, Pages 97-100<br />
387
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Document Type : Letter<br />
Keywords : Cellulosimicrobium; Corneal ulcer; Infectious keratitis; Molecular identification; Stenotrophomonas<br />
<strong>Abstract</strong> : –<br />
No.1062<br />
Author (s) : Prachayasittikul, S., Pingaew, R., Worachartcheewan, A., Ruchirawat, S., Prachayasittikul, V.<br />
Title : A new sulfoxide analog <strong>of</strong> 1,2,3,6-tetrahydrophenylpyridine and antimicrobial activity<br />
Source : EXCLI Journal, Volume 9, <strong>2010</strong>, Pages 102-107<br />
Document Type : Article<br />
Keywords : 4-phenylpyridine l-oxide; Antimicrobial activity; Sulfonium ion; Sulfoxide; Tetrahydropyridine<br />
<strong>Abstract</strong> : Bioactivities <strong>of</strong> thiotetrahydropyridines were previously described. Herein, a novel bioactive sulfoxide analog; N-acetyl-2-(1adamantylsulfoxo)-3-acetoxy-4-phenyl-6-hydroxy-1,2,3,6-tet<br />
rahydropyridine (3) from the deoxydative substitution <strong>of</strong> 4phenylpyridine<br />
1-oxide is reported. Its structure was elucidated using spectral data including 2D-NMR, MS, IR and UV. The<br />
sulfoxide 3 exhibited antibacterial activity against Moraxella catarrhalis and Streptococcus pyogenes with minimum inhibitory<br />
concentration <strong>of</strong> 128 and 256 μg/mL, respectively.<br />
No.1063<br />
Author (s) : Prachayasittikul, S., Saraban, P., Cherdtrakulkiat, R., Ruchirawat, S., Prachayasittikul, V.<br />
Title : New bioactive triterpenoids and antimalarial activity <strong>of</strong> Diospyros rubra Lec.<br />
Source : EXCLI Journal, Volume 9, <strong>2010</strong>, Pages 1-10<br />
Document Type : Article<br />
Keywords : Antimalarial and cytotoxic activities; Antimicrobial; Diospyros rubra Lec.; Triterpenoids<br />
<strong>Abstract</strong> : The first investigation <strong>of</strong> the chemical constituents and bioactivities <strong>of</strong> Diospyros rubra Lec. is reported. D. rubra extracts were<br />
screened for antimicrobial, antimalarial and cytotoxic activities. They were only shown to be active antimalarials. The extracts<br />
with good antimalarial activity were isolated and extensively purified to give lupeol (1), lupenone (2), betulin (3), lupeol<br />
acetate (4), 28-O-acetylbetulin (5), β-sitosteryl-3-O-β-D-glucopyranoside (6) and a mixture <strong>of</strong> β-sitosterol and stigmasterol.<br />
Some <strong>of</strong> the isolates were tested for antimicrobial and cytotoxic actions. Betulin (3) displayed antimicrobial activity against<br />
Streptococcus pyogenes with a minimum inhibitory concentration (MIC) <strong>of</strong> 85 μg/mL. Interestingly, bioactive fractions all<br />
selectively exerted some antimicrobial activity against Corynebacterium diphtheriae NCTC 10356 with the MIC range <strong>of</strong> 64-256<br />
μg/mL. The study provides data to support the medicinal importance <strong>of</strong> the D. rubra.<br />
No.1064<br />
Author (s) : Prachayasittikul, S., Wongsawatkul, O., Suksrichavalit, T., Ruchirawat, S., Prachayasittikul, V.<br />
Title : Bioactivity evaluation <strong>of</strong> eclipta prostrata linn: A potential vasorelaxant<br />
Source : European Journal <strong>of</strong> Scientific Research, Volume 44, Issue 2, August <strong>2010</strong>, Pages 167-176<br />
Document Type : Article<br />
Keywords : Antimicrobials; Antioxidants; Cytotoxic effect; Eclipta prostrata Linn; Nitric oxide; Thoracic aorta; Vasorelaxation<br />
<strong>Abstract</strong> : Eclipta prostrata Linn (Compositae), a traditional medicine has been used for treatment <strong>of</strong> diverse symptoms e.g.<br />
hyperlipidemia, atherosclerosis and skin diseases. This study, hexane extract <strong>of</strong> E. prostrata Linn was evaluated for<br />
bioactivities. The extract showed its new action as a potential vasorelaxant. Moreover, antioxidative (SOD and DPPH assays)<br />
and cytotoxic activities <strong>of</strong> the extract were also observed. Isolation <strong>of</strong> the extract provided a stigmasterol including eight<br />
fractions <strong>of</strong> triterpenoids along with long chain hydrocarbons. All the fractions exhibited antimicrobial activity with minimum<br />
inhibitory concentration (MIC) range <strong>of</strong> 64-256 μg/mL. The results imply therapeutic value <strong>of</strong> the plant species as an<br />
alternative medicinal source <strong>of</strong> potential vasorelaxants. © EuroJournals Publishing, Inc. <strong>2010</strong>.<br />
No.1065<br />
Author (s) : Prachayasittikul, S., Wongsawatkul, O., Worachartcheewan, A., Nantasenamat, C., Ruchirawat, S., Prachayasittikul, V.<br />
Title : Elucidating the Structure-Activity relationships <strong>of</strong> the vasorelaxation and antioxidation properties <strong>of</strong> thionicotinic acid<br />
derivatives<br />
Source : Molecules, Volume 15, Issue 1, January <strong>2010</strong>, Pages 198-214<br />
Document Type : Article<br />
Keywords : 1-adamantylthionicotinic acid and derivatives; Antioxidants; Molecular modeling; Nitric oxide; Prostacyclin; Vasorelaxants<br />
388
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : Nicotinic acid, known as vitamin B3, is an effective lipid lowering drug and intense cutaneous vasodilator. This study reports<br />
the effect <strong>of</strong> 2-(1-adamantylthio)nicotinic acid (6) and its amide 7 and nitrile analog 8 on phenylephrine-induced contraction<br />
<strong>of</strong> rat thoracic aorta as well as antioxidative activity. It was found that the tested thionicotinic acid analogs 6-8 exerted<br />
maximal vasorelaxation in a dose-dependent manner, but their effects were less than acetylcholine (ACh)-induced nitric oxide<br />
(NO) vasorelaxation. The vasorelaxations were reduced, apparently, in both NG-nitro-L- arginine methyl ester (L-NAME) and<br />
indomethacin (INDO). Synergistic effects were observed in the presence <strong>of</strong> L-NAME plus INDO, leading to loss <strong>of</strong><br />
vasorelaxation <strong>of</strong> both the ACh and the tested nicotinic acids. Complete loss <strong>of</strong> the vasorelaxation was noted under removal<br />
<strong>of</strong> endothelial cells. This infers that the vasorelaxations are mediated partially by endothelium-induced NO and prostacyclin.<br />
The thionicotinic acid analogs all exhibited antioxidant properties in both 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and<br />
superoxide dismutase (SOD) assays. Significantly, the thionicotinic acid 6 is the most potent vasorelaxant with ED50 <strong>of</strong> 21.3 nM<br />
and is the most potent antioxidant (as discerned from DPPH assay). Molecular modeling was also used to provide mechanistic<br />
insights into the vasorelaxant and antioxidative activities. The findings reveal that the thionicotinic acid analogs are a novel<br />
class <strong>of</strong> vasorelaxant and antioxidant compounds which have potential to be further developed as promising therapeutics.<br />
No.1066<br />
Author (s) : Prachayasittikul, S., Wongsawatkul, O., Worachartcheewan, A., Ruchirawat, S., Prachayasittikul, V.<br />
Title : Vasorelaxation and superoxide scavenging activities <strong>of</strong> orotic acid<br />
Source : <strong>International</strong> Journal <strong>of</strong> Pharmacology, Volume 6, Issue 4, <strong>2010</strong>, Pages 375-380<br />
Document Type : Article<br />
Keywords : Antioxidants; Indomethacin; L-NAME; Nitric oxide; Orotic acid; Prostacyclin; Vasorelaxants<br />
<strong>Abstract</strong> : The aim <strong>of</strong> study is to investigate effects <strong>of</strong> orotic acid (OA) on phenylephrine-induced contraction <strong>of</strong> rat thoracic aorta and its<br />
antioxidative activity. Results showed that the OA exhibited maximal vasorelaxation in dose-dependent manner with ED50 <strong>of</strong><br />
3.1?×10-7 M, but the effect was less than those <strong>of</strong> acetylcholine (ACh)-induced nitric oxide (NO) vasorelaxation. Significant<br />
reductions <strong>of</strong> the vasorelaxations were found in the presence <strong>of</strong> either NG-nitro-L-arginine methyl ester (L-NAME) or<br />
indomethacin (INDO). Synergistic effects were observed in the presence <strong>of</strong> L-NAME plus INDO that led to loss <strong>of</strong><br />
vasorelaxation <strong>of</strong> both the ACh and the OA. In addition, complete loss <strong>of</strong> the vasorelaxation was manifested under removal<br />
<strong>of</strong> endothelial cells. This implies that the vasorelaxations are mediated by partially endothelium-induced NO and<br />
prostacyclin. The OA exhibited antioxidative activity in both DPPH and SOD assays. The significant results reveal novel actions<br />
<strong>of</strong> the OA as vasorelaxants and superoxide scavenger which are benefits as therapeutic uses and health supplements. © <strong>2010</strong><br />
Asian Network for Scientific Information.<br />
No.1067<br />
Author (s) : Prachopchok, P., Thanachayanont, C., Sriprapha, K., Heawchin, A., Kaewket, S., Vijitjanya, P., Sac-kung, C., Osotchan, T.<br />
Title : Effect <strong>of</strong> annealing on material properties <strong>of</strong> both electrodes in dye sensitized solar cell structure<br />
Source : Advanced Materials Research, Volume 93-94, <strong>2010</strong>, Pages 587-590<br />
Document Type : Conference Paper<br />
Keywords : Annealing; Dye sensitized solar cell; Transparent electrodes<br />
<strong>Abstract</strong> : In dye sensitized solar cell structure, the transparent electrodes <strong>of</strong> tin oxide doped fluorine (SnO2:F) was coated by titanium<br />
dioxide (TiO2) and platinum (Pt) for cathode and anode electrodes, respectively. In order to achieve high efficiency solar cell,<br />
both electrodes are required to have proper crystal structure size and morphology. These can be modified during the<br />
annealing process therefore the effects <strong>of</strong> electrode annealing on their crystal structure and surface modification were<br />
investigated in this study. Thick films <strong>of</strong> TiO2 and Pt were deposited by screen printing method on 3mm thick glass substrate<br />
(Nippon Sheet Glass) coated with 500nm thick SnO2:F. The glass substrate has sheet resistance <strong>of</strong> 20 ohm/square with the<br />
optical transmission <strong>of</strong> about 70%. The mixed TiO2 powder has the diameter <strong>of</strong> about 20 nm. The screen print structure was<br />
heated for drying in the oven at 150°C for 1 hour. Then the TiO2 thick films were annealed at various temperatures from 400<br />
to 550°C for 2 hours, while the Pt films were annealed at lower temperature from 300 to 500oC. The obtained thickness <strong>of</strong><br />
TiO2 and Pt film after annealing become about 10 and 3 μm, respectively. The crystallinity <strong>of</strong> the films was examined by xray<br />
diffraction while the surface morphology <strong>of</strong> both films was determined by atom force microscopy. To investigate the<br />
relation between material structure and the performance <strong>of</strong> the solar cell, the annealed electrodes at different temperature<br />
were used to fabricate the dye sensitized solar cell structure with standard rutherium(II) (N719) dye and then the current<br />
voltage characteristic was measured under light with air mass <strong>of</strong> 1.5. It found that the structure with higher anneal<br />
389
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
temperature electrode exhibited higher power conversion efficiency originating from the higher short circuit current density <strong>of</strong><br />
better crystallinity and higher surface area. © (<strong>2010</strong>) Trans Tech <strong>Publications</strong>.<br />
No.1068<br />
Author (s) : Pramong, R.; Wongchitrat, P.; Govitrapong, P.; Phansuwan-Pujito, P.<br />
Title : EXPRESSIONS OF PERIOD1 AND BRAIN-DERIVED NEUROTROPHIC FACTOR IN THE HIPPOCAMPUS OF POSTNATAL RAT<br />
Source : JOURNAL OF NEUROCHEMISTRY, Volume: 115, Supplement: 1, Pages: 15-15, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1069<br />
Author (s) : Pramyothin, P., Pithukpakorn, M., Arakaki, R.F.<br />
Title : A 47, XXY patient and Xq21.31 duplication with features <strong>of</strong> Prader-Willi syndrome: Results <strong>of</strong> array-based comparative<br />
genomic hybridization<br />
Source : Endocrine, Volume 37, Issue 3, June <strong>2010</strong>, Pages 379-382<br />
Document Type : Article<br />
Keywords : DNA microarrays; Klinefelter syndrome; Prader-Willi-like syndrome; Xq duplication<br />
<strong>Abstract</strong> : A man diagnosed with 47, XXY during childhood presents an appearance similar to that <strong>of</strong> Prader-Willi syndrome with<br />
hypogonadism and gynecomastia, developmental delay, and short stature and obesity. Array-based comparative genomic<br />
hybridization revealed duplication at Xq21.31 in addition to his abnormal karyotype. This duplication was also found in his<br />
mother who appeared normal. We raise the possibility that the phenotype in this patient is a combination <strong>of</strong> both extra X<br />
chromosome and Xq21 duplication. © <strong>2010</strong> Springer Science+Business Media, LLC.<br />
No.1070<br />
Author (s) : Prasansiang, C.; Sujjanant, J.; Anamnart, C.; Jitpratoom, P.; Siritho, S.; Prayoonwiwat, N.<br />
Title : Clinical features <strong>of</strong> multiple sclerosis in Thailand<br />
Source : MULTIPLE SCLEROSIS, Volume: 16, Issue: 10, Pages: 1274-1274, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1071<br />
Author (s) : Prasitpong, S., Chitaree, R.<br />
Title : What Thai students think about directions and types <strong>of</strong> frictional forces<br />
Source : AIP Conference Proceedings, Volume 1263, <strong>2010</strong>, Pages 66-69<br />
Document Type : Conference Paper<br />
Keywords : Alternative conceptions; Forces; The frictional force<br />
<strong>Abstract</strong> : The frictional force is one <strong>of</strong> the obstacles to learning in physics. By itself, this topic consists <strong>of</strong> complicated ideas. In this<br />
study, we have investigated Thai students' ideas about directions and types <strong>of</strong> frictional forces by using open-ended questions<br />
and interviews. For example, these students believed that the direction <strong>of</strong> the frictional force always opposes to that <strong>of</strong> the<br />
motion or that <strong>of</strong> the external force exerted to such object. Moreover, most students thought that the frictional forces are<br />
resistant forces involving the object movement. They did not realize that sometimes the frictional force causes the object to<br />
move; has the same direction <strong>of</strong> the motion as well. About the kinetic friction, most students thought that if an object<br />
moves, it will always have the kinetic friction at the contact areas. From what we have found, we suggest that important<br />
steps for improving students' understanding <strong>of</strong> frictional forces is to teach students to draw free body diagrams, which can<br />
help students to visualize all forces acting on a single object. © <strong>2010</strong> American Institute <strong>of</strong> Physics.<br />
No.1072<br />
Author (s) : Prasitpong, S., Chitaree, R., Rakkapao, S.<br />
Title : Studying the frictional force directions via bristles<br />
Source : Physics Education, Volume 45, Issue 6, November <strong>2010</strong>, Pages 602-610<br />
390
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Document Type : Article<br />
Keywords : –<br />
<strong>Abstract</strong> : We present simple apparatus designed to help Thai high school students visualize the directions <strong>of</strong> frictional forces. Bristles <strong>of</strong><br />
toothbrushes, paintbrushes and scrubbing brushes are used to demonstrate the frictional forces acting in a variety <strong>of</strong><br />
situations. These demonstrations, when followed by discussion <strong>of</strong> free-body diagrams, were found to be effective in teaching<br />
frictional force directions. © <strong>2010</strong> IOP Publishing Ltd.<br />
No.1073<br />
Author (s) : Prasopkittikun, T., Tilokskulchai, F.<br />
Title : Self-efficacy in Infant Care Scale: Revision and further psychometric testing<br />
Source : Nursing and Health Sciences, Volume 12, Issue 4, December <strong>2010</strong>, Pages 450-455<br />
Document Type : Article<br />
Keywords : Infant care; Instrument development; Psychometric testing; Self-efficacy; Thailand<br />
<strong>Abstract</strong> : Maternal self-efficacy is an important factor in parenting behaviors and skills. In order to assess maternal self-efficacy in infant<br />
care, a solid measure is needed. The integrity <strong>of</strong> the assessment <strong>of</strong> self-efficacy in infant care depends upon whether or not<br />
an instrument possesses proper measurement properties. The purpose <strong>of</strong> this study was to revise the scale items and<br />
examine the further psychometric properties <strong>of</strong> the Self-efficacy in Infant Care Scale. Using a sample <strong>of</strong> 235 Thai mothers, the<br />
revised Scale, with 44 items, was found to have internal consistency and test-retest reliability values <strong>of</strong> 0.96 and 0.93,<br />
respectively. A confirmatory factor analysis supported the hypothesized structure. Also, the revised Scale was found to be<br />
correlated with a measure <strong>of</strong> a theoretically related construct; that is, the Parenting Stress Index/Short Form. The<br />
psychometric testing results suggest that the revised Scale can be used as an assessment tool for both research and clinical<br />
purposes. © <strong>2010</strong> Blackwell Publishing Asia Pty Ltd.<br />
No.1074<br />
Author (s) : Prathanturarug, S.; Thirawarapan, S.; Suvitayavat, W.; Soonthornchareonnon, N.; Piyachaturawat, P.; Saralamp, P.<br />
Title : Standardization and pharmacological activities <strong>of</strong> a Curcuma comosa traditional formula for menopausal women<br />
Source : PLANTA MEDICA, Volume: 76, Issue: 12, Pages: 1218-1218, Published: AUG <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1075<br />
Author (s) : Prayoonwiwat, N.<br />
Title : Complementary Approaches to MS and Practical Management <strong>of</strong> MS in Asia: Southeast Asia<br />
Source : MULTIPLE SCLEROSIS, Volume: 16, Issue: 2, Pages: 261-262, Published: FEB <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1076<br />
Author (s) : Prayoonwiwat, N.; Nidhinandana, S.; Chankrachang, S.; Asawavichienjinda, T.; Tantirittisak, T.; Fredrikson, S.; Wicklein, E. M.;<br />
Scherer, P.; Langdon, D.<br />
Title : Psychosocial Aspects <strong>of</strong> Clinically Isolated Syndrome (CIS) in Asia: Baseline Data from the CogniCIS Study Asian Cohort<br />
Source : MULTIPLE SCLEROSIS, Volume: 16, Issue: 2, Pages: 266-267, Published: FEB <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1077<br />
Author (s) : Prayoonwiwat, N.; Siritho, S.; Chankrachang, S.; Nidhinandana, S.; Asawavichienjinda, T.; Tantirittisak, T.; Fredrikson, S.; Wicklein,<br />
E. M.; Langdon, D.<br />
391
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Cognitive function in patients with clinically isolated syndrome suggestive <strong>of</strong> multiple sclerosis in Thailand: 2-year data from<br />
CogniCIS, a worldwide longitudinal study<br />
Source : MULTIPLE SCLEROSIS, Volume: 16, Issue: 10, Pages: 1276-1276, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1078<br />
Author (s) : Prayoonwiwat, N.; Tiamkao, S.; Asawavichienjinda, T.; Tantirittisak, T.; Chankrachang, S.; Tintore, M.; Hagstromer, M.; Wicklein, E.<br />
M.; Penner, I. K.; Sjostrom, M.<br />
Title : Assessment <strong>of</strong> Physical Activity and Fitness in Patients with Early Multiple Sclerosis from Southeast Asia: Baseline Data from<br />
the Betaferon (R) Treatment and Exercise Data Gathering IN Early MS (BEGIN) Study<br />
Source : MULTIPLE SCLEROSIS, Volume: 16, Issue: 2, Pages: 264-264, Published: FEB <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1079<br />
Author (s) : Preativatanyou, K., Sirisup, N., Payungporn, S., Poovorawan, Y., Thavara, U., Tawatsin, A., Sungpradit, S., Siriyasatien, P.<br />
Title : Mitochondrial DNA-based identification <strong>of</strong> some forensically important blowflies in Thailand<br />
Source : Forensic Science <strong>International</strong>, Volume 202, Issue 1-3, October <strong>2010</strong>, Pages 97-101<br />
Document Type : Article<br />
Keywords : Blowflies; Cytochrome oxidase; Mitochondrial DNA; PCR-RFLP; Phylogenetic analysis<br />
<strong>Abstract</strong> : Accurate identification <strong>of</strong> insects collected from death scenes provides not only specific developmental data assisting<br />
forensic entomologists to determine the postmortem interval more precisely but also other kinds <strong>of</strong> forensic evidence.<br />
However, morphological identification can be complicated due to the similarity among species, especially in the early larval<br />
stages. To simplify and make the species identification more practical and reliable, DNA-based identification is preferentially<br />
considered. In this study, we demonstrate the application <strong>of</strong> partial mitochondrial cytochrome oxidase I (COI) and<br />
cytochrome oxidase II (COII) sequences for differentiation <strong>of</strong> forensically important blowflies in Thailand; Chrysomya<br />
megacephala, Chrysomya rufifacies and Lucilia cuprina by polymerase chain reaction-restriction fragment length<br />
polymorphism (PCR-RFLP). The PCR yields a single 1324bp-sized amplicon in all blowfly specimens, followed by direct DNA<br />
sequencing. TaqαI and VspI predicted from the sequencing data provide different RFLP pr<strong>of</strong>iles among these three species.<br />
Sequence analysis reveals no significant intraspecific divergence in blowfly specimens captured from different geographical<br />
regions in Thailand. Accordingly, neighbor-joining tree using Kimura's 2-parameter model illustrates reciprocal monophyly<br />
between species. Thus, these approaches serve as promising tools for molecular identification <strong>of</strong> these three common<br />
forensically important blowfly species in Thailand. © <strong>2010</strong> Elsevier Ireland Ltd.<br />
No.1080<br />
Author (s) : Precharattana, M., Triampo, W., Modchang, C., Triampo, D., Lenbury, Y.<br />
Title : Investigation <strong>of</strong> spatial pattern formation involving CD4+ T cells in HIV/AIDS dynamics by a stochastic cellular automata<br />
model<br />
Source : <strong>International</strong> Journal <strong>of</strong> Mathematics and Computers in Simulation, Volume 4, Issue 4, <strong>2010</strong>, Pages 135-143<br />
Document Type : Article<br />
Keywords : Cellular automata simulation; Hiv; Leukapheresis; Monte carlo; Stochastic process<br />
<strong>Abstract</strong> : In recent years, discrete models have emerged to play an important role in the study <strong>of</strong> immune response especially in the<br />
problem involving human immunodeficiency virus (HIV) infection, leading to AIDS. As infection <strong>of</strong> target immune cells by HIV<br />
mainly takes place in the lymphoid tissue, cellular automata (CA) models thus represent a significant step toward<br />
understanding how the infected population is dispersed. Motivated by these considerations, we introduce a stochastic CA<br />
model for HIV dynamics and explore the spatiotemporal pattern <strong>of</strong> infection. The model is successful in reproducing typical<br />
evolution <strong>of</strong> HIV which is observed in the dynamics <strong>of</strong> CD4+T cells and infected CD+T cells in infected patients. The<br />
geographical result on cell distributions illustrates how infected cells can be dispersed by spatial communities. We have<br />
found the pattern formation is based on the relationship among cell states, the set <strong>of</strong> local transition rules, the conditions<br />
392
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
and the parameters in the systems. The main finding is that the emergence <strong>of</strong> dead cells barriers greatly controls the pattern<br />
formation in our system, by limiting infections and the manner in which the infection dynamics is brought to the last phase<br />
after the barrier is destroyed.<br />
No.1081<br />
Author (s) : Precharattana, M., Triampo, W., Modchang, C., Triampo, D., Lenbury, Y.<br />
Title : Simulation <strong>of</strong> a stochastic cellular automata HIV/AIDS model for investigation <strong>of</strong> spatial pattern formation mediated by CD4+<br />
T cells and HIV dynamics<br />
Source : <strong>International</strong> Conference on Applied Computer Science - Proceedings, <strong>2010</strong>, Pages 375-380<br />
Document Type : Conference Paper<br />
Keywords : Cellular automata; HIV; Leukapheresis; Monte Carlo simulation; Stochastic process<br />
<strong>Abstract</strong> : As infection <strong>of</strong> target immune cells by HIV mainly takes place in the lymphoid tissue, cellular automata (CA) models thus<br />
represent a significant step <strong>of</strong> understanding when the infected population is dispersed over the tissue. Motivated by these<br />
considerations, we have introduced a stochastic CA model for HIV dynamics and, particularly, explored its spatiotemporal<br />
pattern <strong>of</strong> infection. In good agreement, the model is successful to reproduce the typical evolution <strong>of</strong> HIV which is observed<br />
in the dynamics <strong>of</strong> CD4+T cells and infected CD+T cells in infected patients. The geographical result illustrates how infected<br />
cell distributions can be dispersed by spatial community. We have found that pattern formation is based on the relationship<br />
among cell states, the set <strong>of</strong> local transition rules, the conditions and the parameters in the system. The main finding is that<br />
the characteristics <strong>of</strong> dead cells barriers, which greatly control pattern formation in our system, take part in limiting the<br />
spread <strong>of</strong> infection, as well as in bringing the system dynamics toward the end phase <strong>of</strong> the time course <strong>of</strong> infection.<br />
No.1082<br />
Author (s) : Preechagoon, D., Sumyai, V., Chulavatnatol, S., Kulvanich, P., Tessiri, T., Tontisirin, K., Pongjanyakul, T., Uchaipichat, V.,<br />
Aumpon, S., Wongvipaporn, C.<br />
Title : Formulation development <strong>of</strong> morphine sulfate sustained-release tablets and its bioequivalence study in healthy thai<br />
volunteers<br />
Source : AAPS PharmSciTech, Volume 11, Issue 3, September <strong>2010</strong>, Pages 1449-1455<br />
Document Type : Article<br />
Keywords : bioequivalence; formulation development; morphine sulfate; sustained-release tablet<br />
<strong>Abstract</strong> : The objectives <strong>of</strong> this study were to develop morphine sulfate sustained-release tablet formulations and to evaluate the<br />
bioequivalence compared with a commercial brand. The physicochemical properties <strong>of</strong> the formulated and commercial<br />
tablets were determined and compared. The bioequivalence investigation was carried out in 15 healthy male volunteers who<br />
received a single dose in a randomized two-way crossover design. After dosing, serial blood samples were collected for a<br />
period <strong>of</strong> 24 h. Morphine concentration was assayed by high-performance liquid chromatography with electrochemical<br />
detector. The log-transformed C max and AUCs were statistically compared by analysis <strong>of</strong> variance, and the 90% confidence<br />
intervals (CIs) <strong>of</strong> the ratio <strong>of</strong> the log-transformed C max and AUCs between the most promising developed formulation and<br />
the commercial product were determined. It was found that the dissolution rate pr<strong>of</strong>ile <strong>of</strong> a developed formulation was<br />
similar to the commercial brand. Their similarity and difference factors were well within limits. In the bioequivalence study,<br />
the AUC last and AUCinf between the test and the reference products were not statistically different (p=0.227 and p=0.468,<br />
respectively), with the 90% CIs <strong>of</strong> 83.4-102.6% and 87.7-139.4%, respectively. However, the C max <strong>of</strong> the two formulations<br />
was significantly different (p=0.019). The 90% CI <strong>of</strong> the developed formulation was 72.0-93.0% compared to the commercial<br />
product. In vitro dissolution <strong>of</strong> locally prepared morphine sulfate sustained-release tablets was comparable to commercial<br />
brand. However, the results justified the conclusion <strong>of</strong> lack <strong>of</strong> bioequivalence <strong>of</strong> the developed product to the commercial<br />
one. © <strong>2010</strong> American Association <strong>of</strong> Pharmaceutical Scientists.<br />
No.1083<br />
Author (s) : Preechapornkul, P., Chotivanich, K., Imwong, M., Dondorp, A.M., Lee, S.J., Day, N.P.J., White, N.J., Pukrittayakamee, S.<br />
Title : Optimizing the culture <strong>of</strong> plasmodium falciparum in hollow fiber bioreactors<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 4, July <strong>2010</strong>, Pages 761-769<br />
Document Type : Article<br />
Keywords : Ex vivo culture; Hollow fiber bioreactor; Plasmodium falciparum<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : The hollow fiber bioreactor (HFBR) is a cell culturing system allowing continuous perfusion <strong>of</strong> medium. It was designed to<br />
grow microorganisms in a dynamically altering medium mimicking change in the in vivo intravascular and extravascular<br />
compartments. The cell compartment (extra capillary space) and medium compartment (intra capillary space) are connected<br />
through pores <strong>of</strong> semipermeable fiber membranes. These membranes allow exchange <strong>of</strong> gas and nutrients. We have adapted<br />
this system for the ex vivo culture <strong>of</strong> Plasmodium falciparum at high parasite densities. A Thai P. falciparum isolate (TM036)<br />
cultured in RPMI, supplemented with 0.5% Albumax II, could be maintained continuously in the system by daily changes <strong>of</strong> a<br />
small volumes <strong>of</strong> medium. Under optimized conditions the HFBR cultures attained 8% parasitemia in 40% hematocrit,<br />
thereby providing a total parasite biomass <strong>of</strong> 6.0x109 parasitized erythrocytes. The main problem encountered was clogging<br />
<strong>of</strong> micropores in the hollow fiber system by cellular debris over time. Although 'reverse flushing' partly prevented this, a<br />
larger pore size might be needed to overcome this problem. The system opens new possibilities for the study <strong>of</strong> in vitro drug<br />
sensitivity under conditions mimicking in vivo pharmacokinetics, and the selection <strong>of</strong> anti-malarial drug resistance and<br />
associated parasite biological and genomic changes.<br />
No.1084<br />
Author (s) : Preecharram, S., Jearranaiprepame, P., Daduang, S., Temsiripong, Y., Somdee, T., Fukamizo, T., Svasti, J., Araki, T.,<br />
Thammasirirak, S.<br />
Title : Isolation and characterisation <strong>of</strong> crocosin, an antibacterial compound from crocodile (Crocodylus siamensis) plasma<br />
Source : Animal Science Journal, Volume 81, Issue 3, June <strong>2010</strong>, Pages 393-401<br />
Document Type : Article<br />
Keywords : Antibacterial compound; Crocodile; Crocodylus siamensis; Crocosin; Reptile<br />
<strong>Abstract</strong> : An antibacterial compound from crocodile blood was partially purified and functionally characterised. The freshwater<br />
crocodile (Crocodylus siamensis) plasma with antibacterial activity was partially purified by using a centrifugal concentrator<br />
and reverse phase high powered liquid chromatography, and designated as crocosin. Crocosin exhibits antibacterial activity<br />
toward Salmonella typhi and Staphylococcus aureus. Crocosin is thermostable and resistant to pronase digestion. The<br />
structure <strong>of</strong> crocosin analyzed by mass spectrometry contains repeating units <strong>of</strong> 94 and 136 m/z. Scanning electron<br />
microscopy indicates that crocosin probably penetrates progressively into cytoplasm space, perturbing and damaging bacterial<br />
membranes. Crocosin may provide an early defense mechanism toward bacterial infection in freshwater. © <strong>2010</strong> The Authors.<br />
Journal compilation © <strong>2010</strong> Japanese Society <strong>of</strong> Animal Science.<br />
No.1085<br />
Author (s) : Premasathian, N., Avihingsanon, Y., Ingsathit, A., Pongskul, C., Jittiganont, S., Sumethkul, V.<br />
Title : Risk factors and outcome <strong>of</strong> delayed graft function after cadaveric kidney transplantation: A report from the Thai transplant<br />
registry<br />
Source : Transplantation Proceedings, Volume 42, Issue 10, December <strong>2010</strong>, Pages 4017-4020<br />
Document Type : Conference Paper<br />
Keywords : creatinine, interleukin 2 receptor antibody, thymocyte antibody; brain death, cadaver kidney, cerebrovascular accident, cold<br />
ischemia, conference paper, controlled study, creatinine blood level, delayed graft function, dialysis, gender, graft<br />
survival, human, kidney failure, major clinical study, peritoneal dialysis, priority journal, resuscitation, risk<br />
factor, Thailand, treatment outcome<br />
<strong>Abstract</strong> : Introduction Kidney transplantation is the best treatment for end-stage renal disease patients. Delayed graft function (DGF)<br />
remains one <strong>of</strong> the major problems after cadaveric kidney transplantation. This study has reported the risk factors and<br />
outcomes <strong>of</strong> DGF using data from Thai Transplant Registry Database. Methods The data <strong>of</strong> all cadaveric kidney<br />
transplantations (CD-KT) were retrieved from the database. DGF was defined as a failure to decrease the serum creatinine<br />
within 72 hours or a requirement for dialysis within the first week after transplantation. We performed logistic regression<br />
analysis to correlate donor features (age, sex, cardio-pulmonary resuscitation (CPR), brain death from a cerebrovascular<br />
accident (CVA), best and last serum creatinine) with recipient demographics (age, sex) and clinical outcomes cold ischemic<br />
time [CIT] and DGF. Results We analyzed 756 CD-KT including 320 (42%) patients experiencing DGF. Upon multivariate<br />
analysis, factors significantly correlated with DGF were CIT (P < .001), donor last serum creatinine (P < .001), interleukin 2<br />
monoclonal antibody induction (P = .004), donor age (P = .017), donor CVA (P = .012), and prior peritoneal dialysis (PD) (P =<br />
.012). There was no significant correlation between DGF and donor height, weight, sex, CPR, brain death from CVA, best serum<br />
creatinine, recipient age, or sex in multivariate analysis. Graft survivals at 1 and 5 years after transplantation were significantly<br />
lower among the DGF group namely, 91.0% vs. 95.2% and 78.7% vs. 86.0%, respectively (P = .006). Patient survival was also<br />
394
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
significantly lower 94.1% vs. 96.4% and 82.1% vs. 92.2%, respectively, (P = .001). Conclusion A higher value <strong>of</strong> the donor's<br />
terminal serum creatinine, CIT, IL2mAb induction, PD prior to KT and donor age increased the risk for DGF after CD-KT. DGF<br />
significantly lowered kidney allograft and patient survivals at 1 and 5 years after transplantation among the Thai population.<br />
© <strong>2010</strong> Elsevier Inc. All rights reserved.<br />
No.1086<br />
Author (s) : Premasathian, N., Vongwiwatana, A., Taweemonkongsap, T., Amornvesukit, T., Limsrichamrern, S., Jitpraphai, S., Kositamongkol,<br />
P., Mahawithitwong, P., Sritippayawan, S., Chanchairujira, T., Nualyong, C., Vareesangthip, K., Vasuvattakul, S., Sirivatanauksorn,<br />
Y.<br />
Title : The 37-year kidney transplantation experience at Siriraj Hospital.<br />
Source : Clinical transplants, <strong>2010</strong>, Pages 141-148<br />
Document Type : Article<br />
Keywords : immunosuppressive agent; adolescent, adult, aged, article, child, chronic kidney failure, delayed graft<br />
function, donor, female, graft rejection, graft survival, histocompatibility test, hospital, hospital<br />
admission, human, immunology, infant, kidney transplantation, laparoscopy, living donor, male, middle<br />
aged, mortality, nephrectomy, newborn, preschool child, statistics, Thailand, time, treatment outcome;<br />
Adolescent, Adult, Aged, Child, Child, Preschool, Delayed Graft Function, Female, Graft Rejection, Graft<br />
Survival, Histocompatibility Testing, Hospitals, Humans, Immunosuppressive Agents, Infant, Infant, Newborn, Kidney Failure,<br />
Chronic, Kidney Transplantation, Laparoscopy, Living Donors, Male, Middle Aged, Nephrectomy, Thailand, Time Factors, Tissue<br />
Donors, Treatment Outcome, Waiting Lists, Young Adult<br />
<strong>Abstract</strong> : With 37-years <strong>of</strong> experience, a total <strong>of</strong> 801 kidney transplantations (59.4% were deceased donors and 40.6% were living<br />
donors) performed at Siriraj hospital were reported. The point system parallel to OPTN/UNOS for waitlists was utilized. Most<br />
<strong>of</strong> the recipients <strong>of</strong> deceased donor kidney transplantations had 3 HLA mismatches. Due to the point allocation system, none<br />
<strong>of</strong> them had 6 HLA mismatches. Extended criteria donor comprised 7.8% <strong>of</strong> all deceased donors. Mean duration <strong>of</strong> dialysis<br />
prior to deceased donor transplant was 53 +/- 34 months. Delayed graft function (DGF) was found in 54% <strong>of</strong> deceased donor<br />
kidney transplantation and resulted in significantly higher rate <strong>of</strong> 1 year biopsy-proven acute rejection, longer duration <strong>of</strong><br />
kidney transplant admission, higher admission cost and lower patient survival compared to those with immediate graft<br />
function. Most <strong>of</strong> living donor kidney transplant recipient had 1 haplotype match. Mean donor age was 35.9 +/- 9.8 years.<br />
95.6% <strong>of</strong> the recipients were on hemodialysis prior to transplantation. The current standard regimen includes calcineurin<br />
inhibitor, Mycophenolic acid and prednisolone. Interleukin-2 receptor monoclonal antibody has been used in the high<br />
immunological risk or high risk for DGF recipients that were 50% <strong>of</strong> the recipients. There was no statistically significant<br />
difference in the biopsy-proven acute rejection (BPAR) free survival between deceased and living donor transplantation.<br />
Proportion <strong>of</strong> cases with the diagnosis <strong>of</strong> acute rejection according to Banff 2007 classification is as follows: 32.4% acute<br />
cellular rejection (ACR), 39.4% antibody-mediated rejection (AMR) and 21.1% mixed cellular and antibody-mediated rejection.<br />
Seventy two patients, 35 deceased donor and 37 living donor kidney transplant recipients, had biopsy-proven glomerular<br />
disease after transplantation which IgA nephropathy is the most common form <strong>of</strong> glomerulonephritis. Median graft survival<br />
was 7.6 and 13.2 years and median patient survival was 12.1 and 15.5 years for recipient <strong>of</strong> deceased and living donor<br />
transplant respectively. The follow up program <strong>of</strong> living donors was introduced in 2003 and there were not any donors who<br />
required renal replacement therapy.<br />
No.1087<br />
Author (s) : Preutthipan, S., Herabutya, Y.<br />
Title : Hysteroscopic rollerball endometrial ablation as an alternative treatment for adenomyosis with menorrhagia and/or<br />
dysmenorrhea<br />
Source : Journal <strong>of</strong> Obstetrics and Gynaecology Research, Volume 36, Issue 5, October <strong>2010</strong>, Pages 1031-1036<br />
Document Type : Article<br />
Keywords : adenomyosis; dysmenorrhea; endometrial ablation; hysteroscopic surgery; menorrhagia; rollerball ablation<br />
<strong>Abstract</strong> : Aim: The aim <strong>of</strong> this study was to assess the long-term effectiveness and safety <strong>of</strong> hysteroscopic rollerball endometrial<br />
ablation as a surgical management <strong>of</strong> adenomyosis with menorrhagia and/or dysmenorrhea. We compared the results <strong>of</strong><br />
patients who underwent pretreatment with gonadotropin-releasing hormone (GnRH) agonist with the results <strong>of</strong> those who did<br />
not. Methods: A retrospective study included 190 adenomyotic patients who suffered from menorrhagia and/or<br />
dysmenorrhea and underwent hysteroscopic rollerball endometrial ablation. Main outcome measures were rates <strong>of</strong><br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
successful operation, complications, improvement <strong>of</strong> abnormal uterine bleeding and pelvic pain after the surgery. Results:<br />
The majority <strong>of</strong> the patients (142, 74.7%) underwent hysteroscopic rollerball endometrial ablation during the early<br />
proliferative phase <strong>of</strong> the menstrual cycle. The rest were operated on after GnRH agonist pretreatment for 6-8 weeks.<br />
Ablations were successfully performed on all patients in a day surgery setting. The average operation time was 36.3 ± 7.1 min.<br />
The mean glycine deficit was 583.4 ± 247.3 mL. The ablation in the no-pretreatment group took a significantly longer time<br />
and had more glycine absorption compared to the GnRH agonist pretreatment group (P < 0.0001). Mean postoperative followup<br />
duration was five years (range 1-10 years). A total <strong>of</strong> 187 patients (98.4%) who had undergone hysteroscopic endometrial<br />
ablation reported decreased bleeding: amenorrhea in 58 (30.5%), hypomenorrhea in 78 (41.1%), and eumenorrhea in 51<br />
(26.8%) patients. Three patients (1.6%) underwent hysterectomy due to symptoms recurrence. A total <strong>of</strong> 165 (86.8%) patients<br />
with dysmenorrhea reported either reduced or no dysmenorrhea. There was no significant difference in the efficacy <strong>of</strong><br />
hysteroscopic rollerball endometrial ablation between patients who underwent pretreatment with GnRH agonists and those<br />
who did not. No major complications related to the procedure were reported. Conclusions: Hysteroscopic rollerball<br />
endometrial ablation as a surgical management <strong>of</strong> menorrhagia and dysmenorrhea that develops in patients with<br />
adenomyosis is an effective and safe procedure. It can reduce the need for the unnecessary major surgery <strong>of</strong> hysterectomy.<br />
© <strong>2010</strong> Japan Society <strong>of</strong> Obstetrics and Gynecology.<br />
No.1088<br />
Author (s) : Price, E.P., Hornstra, H.M., Limmathurotsakul, D., Max, T.L., Sarovich, D.S., Vogler, A.J., Dale, J.L., Ginther, J.L., Leadem, B.,<br />
Colman, R.E., Foster, J.T., Tuanyok, A., Wagner, D.M., Peacock, S.J., Pearson, T., Keim, P.<br />
Title : Within-host evolution <strong>of</strong> Burkholderia pseudomallei in four cases <strong>of</strong> acute melioidosis<br />
Source : PLoS Pathogens, Volume 6, Issue 1, January <strong>2010</strong>, Article number e1000725<br />
Document Type : Article<br />
Keywords : acute disease, article, bacterial colonization, bacterium culture, bacterium isolate, Burkholderia pseudomallei, case<br />
report, clinical feature, controlled study, disease course, evolutionary adaptation, genetic variability, genotype, host pathogen<br />
interaction, human, melioidosis, mutational analysis, nonhuman, phenotype, phylogeny, variable number <strong>of</strong> tandem<br />
repeat, adult, bacterial gene, female, genetics, male, middle aged, molecular evolution, molecular<br />
genetics, mutation, nucleotide sequence, pulsed field gel electrophoresis; Bacteria (microorganisms), Burkholderia<br />
pseudomallei; Adult, Base Sequence, Burkholderia pseudomallei, Electrophoresis, Gel, Pulsed-Field, Evolution,<br />
Molecular, Female, Genes, Bacterial, Genotype, Humans, Male, Melioidosis, Middle Aged, Minisatellite Repeats, Molecular<br />
Sequence Data, Mutation, Phylogeny<br />
<strong>Abstract</strong> : Little is currently known about bacterial pathogen evolution and adaptation within the host during acute infection. Previous<br />
studies <strong>of</strong> Burkholderia pseudomallei, the etiologic agent <strong>of</strong> melioidosis, have shown that this opportunistic pathogen<br />
mutates rapidly both in vitro and in vivo at tandemly repeated loci, making this organism a relevant model for studying shortterm<br />
evolution. In the current study, B. pseudomallei isolates cultured from multiple body sites from four Thai patients with<br />
disseminated melioidosis were subjected to fine-scale genotyping using multilocus variable-number tandem repeat analysis<br />
(MLVA). In order to understand and model the in vivo variable-number tandem repeat (VNTR) mutational process, we<br />
characterized the patterns and rates <strong>of</strong> mutations in vitro through parallel serial passage experiments <strong>of</strong> B. pseudomallei.<br />
Despite the short period <strong>of</strong> infection, substantial divergence from the putative founder genotype was observed in all four<br />
melioidosis cases. This study presents a paradigm for examining bacterial evolution over the short timescale <strong>of</strong> an acute<br />
infection. Further studies are required to determine whether the mutational process leads to phenotypic alterations that<br />
impact upon bacterial fitness in vivo. Our findings have important implications for future sampling strategies, since colonies in<br />
a single clinical sample may be genetically heterogeneous, and organisms in a culture taken late in the infective process may<br />
have undergone considerable genetic change compared with the founder inoculum. © <strong>2010</strong> Price et al.<br />
No.1089<br />
Author (s) : Promklay, A., Fuangtharnthip, P., Surarit, R., Atsawasuwan, P.<br />
Title : Response <strong>of</strong> dental pulp cells to Er:YAG irradiation<br />
Source : Photomedicine and Laser Surgery, Volume 28, Issue 6, 1 December <strong>2010</strong>, Pages 793-799<br />
Document Type : Article<br />
Keywords : Cavity preparations, Cell surfaces, Critical thickness, Dental pulp, Dental pulp cells, Enzyme immunoassay, Er:YAG<br />
irradiation, Er:YAG lasers, Laser irradiations, Low energies, Minimal damage, Output energy, Repetition rate, Scanning electron<br />
micrographs, Type I procollagen; Cell membranes, Enzyme activity, Erbium, Irradiation, Irrigation, Laser beam<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
effects, Morphology, Pulp materials, Scanning electron microscopy; Cytology;<br />
Anatomy, Cells, Cytology, Enzymes, Irrigation, Lasers, Membranes, Radiation Effects, Scanning Electron Microscopy; collagen<br />
type 1; adult, article, biosynthesis, cell culture, cytology, enzyme linked immunosorbent assay, human, male, radiation<br />
exposure, scanning electron microscopy, solid state laser, tooth pulp; Cells, Cultured, Collagen Type I, Dental Pulp, Enzyme-<br />
Linked Immunosorbent Assay, Humans, Lasers, Solid-State, Male, Microscopy, Electron, Scanning, Young Adult<br />
<strong>Abstract</strong> : Objective: This study aimed to investigate the response <strong>of</strong> human dental pulp cells lying on a thin dentin disc to Er:YAG<br />
irradiation. Background Data: Er:YAG laser irradiation has been effectively used for tooth cavity preparation with minimal<br />
damage to the dental pulp tissue. However, study <strong>of</strong> its direct effect on pulp cells has been limited. Materials and Methods:<br />
Primary human dental pulp cells were cultured and allowed to grow on one side <strong>of</strong> 500-μm-thick dentin discs. An Er:YAG<br />
laser at output energies <strong>of</strong> a 120, 300, or 500mJ/pulse with a repetition rate <strong>of</strong> 10Hz was used to ablate the non-cell surface<br />
<strong>of</strong> the dentin disc for 10s with cooling irrigation. Results: Twenty-four hours after laser irradiation, light and scanning electron<br />
micrographs revealed pulp cells with a normal fibroblastic morphology for the 120 and 300mJ laser-treated groups. In the<br />
500mJ laser-treated group, many pyknotic cells with knob-like projections on the cell surface were mostly observed: the<br />
number <strong>of</strong> cells with normal morphology decreased compared to that <strong>of</strong> the other groups. However, the production <strong>of</strong> type I<br />
procollagen assessed by the enzyme immunoassay increased in the 500mJ laser-treated group significantly (p
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Transabdominal sonography revealed a male fetus with bilateral hydroureter, hydronephrosis, dilated bladder and normal<br />
amniotic fluid. The fetal penis was enlarged and the penile urethra was dilated with ballooning at the distal end. The urethral<br />
meatus was identified. The fetus was delivered at term with a favorable outcome. Copyright (c) <strong>2010</strong> S. Karger AG, Basel.<br />
No.1092<br />
Author (s) : Prueksaritanond, S., Prueksaritanond, S., Ratanarapee, S., Prueksaritanond, C.<br />
Title : Scrotal pain as the first manifestation <strong>of</strong> scrotal leiomyoma: A case report<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 5, May <strong>2010</strong>, Pages 633-636<br />
Document Type : Article<br />
Keywords : Leiomyoma; Pain; Scrotum; Smooth muscle tumor; Tunica dartos<br />
<strong>Abstract</strong> : Primary scrotal leiomyoma is derived from smooth muscle cells <strong>of</strong> tunica dartos. A 37-year-old Thai man, barber by<br />
pr<strong>of</strong>ession, presented with left scrotal pain and subsequently, a solid mass is reported. As a relatively rare neoplasm, the<br />
initial diagnosis and differential diagnosis are intricate. The management was surgical excision. The well circumscribed 1 cm<br />
mass appeared as homogeneous, whorled-like, with interlacing fascicles <strong>of</strong> spindled muscle cells, and a strong positive<br />
desmin immunoreactivity. Upon follow-up, pain had disappeared one year after surgery. Reassurance and careful reexamination<br />
to reduce anxiety was also needed.<br />
No.1093<br />
Author (s) : Pruksakorn, D., Lirdprapamongkol, K., Chokchaichamnankit, D., Subhasitanont, P., Chiablaem, K., Svasti, J., Srisomsap, C.<br />
Title : Metabolic alteration <strong>of</strong> HepG2 in scaffold-based 3-D culture: Proteomic approach<br />
Source : Proteomics, Volume 10, Issue 21, November <strong>2010</strong>, Pages 3896-3904<br />
Document Type : Article<br />
Keywords : 3-D culture; Cell biology; Creatine kinase; HepG2; Hypoxia<br />
<strong>Abstract</strong> : 3-D cell culture models are important in cancer biology since they provide improved understanding <strong>of</strong> tumor<br />
microenvironment. We have established a 3-D culture model using HepG2 in natural collagen-based scaffold to mimic the<br />
development <strong>of</strong> small avascular tumor in vivo. Morphological characterization showed that HepG2 colonies grew within the<br />
interior <strong>of</strong> the scaffold and showed enhanced extracellular matrix deposition. High levels <strong>of</strong> cell proliferation in the<br />
outermost regions <strong>of</strong> the scaffold created a hypoxic microenvironment in the 3-D culture system, as indicated by hypoxiainducible<br />
factor-1α stabilization, detectable by Western blotting and immunohistochemistry. Proteomic studies showed<br />
decreased expression <strong>of</strong> several mitochondrial proteins and increased expression <strong>of</strong> proteins in anaerobic glycolysis under 3-D<br />
culture compared to monolayer culture. Creatine kinase was also upregulated in 3-D culture, indicating its possible role as an<br />
important energy buffer system under hypoxic microenvironment. Increased levels <strong>of</strong> proteins in nucleotide metabolism may<br />
relate to cellular energy. Thus, our results suggest that HepG2 cells under 3-D culture adapt their energy metabolism in<br />
response to hypoxic conditions. Metabolic alterations in the 3-D culture model may relate to physiological changes relevant<br />
to development <strong>of</strong> small avascular tumor in vivo and their study may improve future therapeutic strategies. © <strong>2010</strong> WILEY-<br />
VCH Verlag GmbH & Co. KGaA, Weinheim.<br />
No.1094<br />
Author (s) : Prutthiwanasan, B., Suntornsuk, L.<br />
Title : Rapid analysis <strong>of</strong> alkylphosphonate drugs by capillary zone electrophoresis using indirect ultraviolet detection<br />
Source : Journal <strong>of</strong> Separation Science, Volume 33, Issue 2, February <strong>2010</strong>, Pages 228-234<br />
Document Type : Article<br />
Keywords : Alendronate; Clodronate; CZE; Fosfomycin; Indirect UV detection<br />
<strong>Abstract</strong> : A rapid capillary electrophoretic method for the analysis <strong>of</strong> three alkylphosphonate drugs (i.e. fosfomycin disodium (FOS),<br />
clodronate disodium (CLO) and alendronate sodium (ALN)) was developed by using multiple probe BGE and indirect UV<br />
detection. BGE containing 30 mM benzoic acid, 5 mM salicylic acid and 0.5 mM CTAB (pH 3.8), temperature <strong>of</strong> 30°1C, applied<br />
voltage <strong>of</strong> -30 kV and detection at 220 nm provided baseline separation <strong>of</strong> all analytes (resolution (R)>2.2) in 3.2 min. EOF<br />
reversal by addition <strong>of</strong> CTAB and negative voltage polarity leading to the co-EOF flow and short analysis time. Two probe BGE<br />
greatly improved peak symmetry. The method showed good linearity (r2>0.999 in ranges <strong>of</strong> 20-1000 μg/mL for FOS, 100-<br />
1000 μg/mL for CLO and 100-750 μg/mL for ALN) repeatablitiy (RSD<2.15%), recovery (99.3-101.1%) and sensitivity<br />
(LOD<50 μg/mL). Freshly prepared BGE and sample solutions are essential for the method precision and accuracy. This<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
new method can be utilized for routine analysis <strong>of</strong> FOS, CLO and ALN in dosage forms because <strong>of</strong> its efficiency, reliability,<br />
speed and simplicity. © <strong>2010</strong> Wiley-VCH Verlag GmbH & Co. KGaA.<br />
No.1095<br />
Author (s) : Pukrittayakamee, S., Imwong, M., Chotivanich, K., Singhasivanon, P., Day, N.P.J., White, N.J.<br />
Title : A comparison <strong>of</strong> two short-course primaquine regimens for the treatment and radical cure <strong>of</strong> Plasmodium vivax malaria in<br />
Thailand<br />
Source : American Journal <strong>of</strong> Tropical Medicine and Hygiene, Volume 82, Issue 4, April <strong>2010</strong>, Pages 542-547<br />
Document Type : Article<br />
Keywords : chloroquine, paracetamol, primaquine, quinine, tetracycline; adolescent, adult, article, clinical trial, comparative<br />
study, controlled clinical trial, controlled study, dose response, drug tolerability, fever, gametocyte, hematocrit, human, major<br />
clinical study, malaria falciparum, male, parasite, parasitemia, Plasmodium vivax malaria, randomized controlled<br />
trial, recurrent disease, relapse, side effect, survival, Thailand, unspecified side effect;<br />
Adolescent, Adult, Animals, Antimalarials, Dose-Response Relationship, Drug, Drug Administration Schedule, Humans, Malaria,<br />
Vivax, Male, Middle Aged, Plasmodium vivax, Primaquine, Thailand, Young Adult; Plasmodium vivax<br />
<strong>Abstract</strong> : Thai adult males (N = 85) with acute Plasmodium vivax malaria and normal glucose-6-phosphate dehydrogenase screening<br />
were randomized to receive 30 mg or 60 mg primaquine daily for 7 days (N = 43 and 42, respectively). The regimens were<br />
well tolerated and all patients recovered fully. Median fever clearance (47 hours; range 4 to 130 hours), mean ± SD parasite<br />
clearance times (87.7 ± 25.3 hours), gametocyte clearance, and adverse effects were similar in the 2 groups. Two patients, 1<br />
from each group, had a 30% reduction in hematocrit. The cumulative 28 day relapse rate (95% confidence interval) by Kaplan<br />
Meier survival analysis was 29% (16-49%) in the 30 mg group compared with 7% (2-24%) in the 60 mg group; P = 0.027.<br />
Comparison with previous data obtained at this same site suggests that the recurrences comprised approximately 17%<br />
recrudescences and 12% relapses in the 30 mg/day group compared with 3% recrudescences and 4% relapses in the 60<br />
mg/day group. These data suggest that the dose-response relationships for primaquine's asexual stage and hypnozoitocidal<br />
activities in-vivo are different. A 1 week course <strong>of</strong> primaquine 60 mg daily is an effective treatment <strong>of</strong> vivax malaria in this<br />
region. Copyright © <strong>2010</strong> by The American Society <strong>of</strong> Tropical Medicine and Hygiene.<br />
No.1096<br />
Author (s) : Pulmanausahakul, R., Khakpoor, A., Smith, D.R.<br />
Title : The development <strong>of</strong> flavivirus vaccines<br />
Source : African Journal <strong>of</strong> Biotechnology, Volume 9, Issue 4, 25 January <strong>2010</strong>, Pages 409-415<br />
Document Type : Review<br />
Keywords : Attenuation; Encephalitis; Fever; Flavivirus; Hemorrhagic; Vaccine<br />
<strong>Abstract</strong> : Mosquito and tick-borne flaviviruses are the causative agents <strong>of</strong> some <strong>of</strong> the world's most important diseases, including<br />
dengue fever, yellow fever, Japanese encephalitis, tick-borne encephalitis and West Nile fever. Cumulatively, these viruses<br />
cause many millions <strong>of</strong> infections each year and impose a significant burden on public health resources, particularly in<br />
developing and newly developed countries. Vaccine development to eliminate flaviviral infections has been marked by<br />
uneven progress and a large number <strong>of</strong> setbacks. To date, no single approach has proved successful in leading to vaccine<br />
development against a wide range <strong>of</strong> flaviviruses, but the application <strong>of</strong> modern techniques to the problem is opening up<br />
new avenues <strong>of</strong> approach. This review summarizes some <strong>of</strong> the developments in vaccine research aimed at inducing<br />
protective immunity against flaviviral infections. © <strong>2010</strong> Academic Journals.<br />
No.1097<br />
Author (s) : Pulsawat, P., Piboonpocanun, S., Sirivichayakul, S., Buranapraditkun, S., Jacquet, A., Shimada, M., Okuda, K., Ruxrungtham, K.<br />
Title : Production and immunogenicity <strong>of</strong> hypoallergenic codon-optimized DNA vaccine encoding mature Der p 1 allergen<br />
Source : Journal <strong>of</strong> Investigational Allergology and Clinical Immunology, Volume 20, Issue 7, <strong>2010</strong>, Pages 582-590<br />
Document Type : Article<br />
Keywords : Codon-optimized DNA vaccine; Der p 1; Hypoallergen; Mature Der p 1<br />
<strong>Abstract</strong> : Background: Genetic vaccination with plasmid DNA encoding allergens is a promising potential approach for the treatment or<br />
prevention <strong>of</strong> allergy. Nonetheless, because the allergens expressed can display immunoglobulin (Ig) E reactivity, methods to<br />
deliver hypoallergenic variants can minimize the risk <strong>of</strong> type 2 helper (TH2) cell priming after DNA immunization. Methods: A<br />
humanized synthetic gene encoding mature Dermatophagoides pteronyssinus group 1 (Der p 1) allergen was cloned into the<br />
pHIS expression vector carrying unmethylated CpG 2006 (CpG 2006) motif but devoid <strong>of</strong> signal sequence. The immunogenicity<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>of</strong> this DNA construct was compared in naïve mice with that <strong>of</strong> recombinant ProDer p 1 protein adjuvanted with alum.<br />
Results: Codon optimization <strong>of</strong> the cDNA encoding mature Der p 1 markedly improved allergen expression. Mature Der p 1,<br />
expressed intracellularly in Human Embryonic Kidney 293 cells (HEK 293 cells) transfected with codon-optimized Der p 1<br />
cDNA (pHIS-mHuDer p 1), was shown to be hypoallergenic as it displayed no IgE reactivity. Intradermal vaccinations <strong>of</strong> naïve<br />
Balb/C mice with pHIS-mHuDer p 1 elicited an allergen-specific TH1 response characterized by the production <strong>of</strong> specific<br />
IgG2a, a very low amount <strong>of</strong> specific IgG1, and no specific IgE. Lipoplex formulation with cationic liposome composed <strong>of</strong><br />
lecithin, N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium methylsulfate (DOTAP) and cholesterol not only accelerated<br />
the induction <strong>of</strong> TH1 response but also increased its intensity. Conclusion: A codon-optimized DNA vaccine encoding mature<br />
Der p 1 in a lipoplex formulation could represent a promising hypoallergenic vaccine candidate for safer immunotherapy<br />
against house dust mite allergy. © <strong>2010</strong> Esmon Publicidad.<br />
No.1098<br />
Author (s) : Pumirat, P., Cuccui, J., Stabler, R.A., Stevens, J.M., Muangsombut, V., Singsuksawat, E., Stevens, M.P., Wren, B.W., Korbsrisate, S.<br />
Title : Global transcriptional pr<strong>of</strong>iling <strong>of</strong> Burkholderia pseudomallei under salt stress reveals differential effects on the Bsa type III<br />
secretion system<br />
Source : BMC Microbiology, Volume 10, <strong>2010</strong>, Article number 171<br />
Document Type : Article<br />
Keywords : article, bacterial gene, bacterial growth, bacterium culture, Burkholderia pseudomallei, controlled study, DNA<br />
fingerprinting, genetic transcription, human, human cell, lung alveolus epithelium, microarray analysis, nonhuman, protein<br />
expression, reverse transcription polymerase chain reaction, salinity, salt stress, upregulation, Western blotting, culture<br />
medium, DNA microarray, drug effect, gene expression pr<strong>of</strong>iling, gene expression<br />
regulation, genetics, metabolism, physiological stress; Burkholderia pseudomallei; sodium chloride; Burkholderia<br />
pseudomallei, Culture Media, Gene Expression Pr<strong>of</strong>iling, Gene Expression Regulation, Bacterial, Oligonucleotide Array<br />
Sequence Analysis, Reverse Transcriptase Polymerase Chain Reaction, Sodium Chloride, Stress, Physiological, Transcription,<br />
Genetic<br />
<strong>Abstract</strong> : Background. Burkholderia pseudomallei is the causative agent <strong>of</strong> melioidosis where the highest reported incidence world wide<br />
is in the Northeast <strong>of</strong> Thailand, where saline soil and water are prevalent. Moreover, recent reports indicate a potential<br />
pathogenic role for B. pseudomallei in cystic fibrosis lung disease, where an increased sodium chloride (NaCl) concentration in<br />
airway surface liquid has been proposed. These observations raise the possibility that high salinity may represent a favorable<br />
niche for B. pseudomallei. We therefore investigated the global transcriptional response <strong>of</strong> B. pseudomallei to increased<br />
salinity using microarray analysis. Results. Transcriptome analysis <strong>of</strong> B. pseudomallei under salt stress revealed several genes<br />
significantly up-regulated in the presence <strong>of</strong> 320 mM NaCl including genes associated with the bsa-derived Type III secretion<br />
system (T3SS). Microarray data were verified by reverse transcriptase-polymerase chain reactions (RT-PCR). Western blot<br />
analysis confirmed the increased expression and secretion <strong>of</strong> the invasion-associated type III secreted proteins BipD and BopE<br />
in B. pseudomallei cultures at 170 and 320 mM NaCl relative to salt-free medium. Furthermore, salt-treated B. pseudomallei<br />
exhibited greater invasion efficiency into the lung epithelial cell line A549 in a manner partly dependent on a functional Bsa<br />
system. Conclusions. B. pseudomallei responds to salt stress by modulating the transcription <strong>of</strong> a relatively small set <strong>of</strong><br />
genes, among which is the bsa locus associated with invasion and virulence. Expression and secretion <strong>of</strong> Bsa-secreted proteins<br />
was elevated in the presence <strong>of</strong> exogenous salt and the invasion efficiency was enhanced. Our data indicate that salinity has<br />
the potential to influence the virulence <strong>of</strong> B. pseudomallei. © <strong>2010</strong> Pumirat et al; licensee BioMed Central Ltd.<br />
No.1099<br />
Author (s) : Punnakitikashem, P., Intarakumnerd, P., Laosirihongthong, T.<br />
Title : Performance assessment <strong>of</strong> technology transfer project: An application <strong>of</strong> DEA technique<br />
Source : IEEM<strong>2010</strong> - IEEE <strong>International</strong> Conference on Industrial Engineering and Engineering Management, <strong>2010</strong>, Article number<br />
5674233, Pages 1027-1031<br />
Document Type : Conference Paper<br />
Keywords : Chief technology <strong>of</strong>ficer; Data envelopment analysis; DEA; Performance measurement; Technology transfer<br />
<strong>Abstract</strong> : Technology transfer is a mechanism to acquire technology and helps company to enhance their technology capabilities. The<br />
purpose <strong>of</strong> this study is to demonstrate how Data Envelopment Analysis (DEA) can be used to assess performance <strong>of</strong><br />
technology transfer project at firm level. Data collected from transferring the robotics operating technology project <strong>of</strong> 12<br />
automotive parts/components manufacturing companies are examined by using the DEA model. The results <strong>of</strong> this study<br />
400
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
show that six companies carried out efficient technology transferring. The average efficiency score <strong>of</strong> technology transfer<br />
project is 93.3%. This finding confirms that absorptive capacity <strong>of</strong> recipient firms is a critical success factor underlying<br />
differences between efficient and inefficient firms. DEA method provides Chief Technology Officers (CTO) or project leaders a<br />
framework to determine appropriate transferring mechanisms and necessary internal and external resources. ©<strong>2010</strong> IEEE.<br />
No.1100<br />
Author (s) : Punnakitikashem, P., Laosirihongthong, T., Adebanjo, D., McLean, M.W.<br />
Title : A study <strong>of</strong> quality management practices in TQM and non-TQM firms: Findings from the ASEAN automotive industry<br />
Source : <strong>International</strong> Journal <strong>of</strong> Quality and Reliability Management, Volume 27, Issue 9, October <strong>2010</strong>, Pages 1021-1035<br />
Document Type : Article<br />
Keywords : Automotive industry; ISO 9000 series; Organizational restructuring; Quality management; South East Asia; Total quality<br />
management<br />
<strong>Abstract</strong> : Purpose: The primary objective <strong>of</strong> this research is to explore whether total quality management (TQM) firms execute various<br />
quality management practices significantly differently from non-TQM firms in the Association <strong>of</strong> South East Asian Nations<br />
(ASEAN) automotive supply chain. The study also aims to analyze differences between different tiers <strong>of</strong> this supply chain and<br />
to examine the relationship between the implementation <strong>of</strong> quality management systems and adoption <strong>of</strong> TQM.<br />
Design/methodology/approach: A total <strong>of</strong> 165 datasets collected from ASEAN automotive Original Equipment Manufacturers<br />
(OEMs) and their tier 1 and 2 suppliers in five ASEAN countries were tested by using cross-tabulation analysis and ANOVA with<br />
post hoc test. Findings: The results show that firms that have successfully implemented the concept <strong>of</strong> work standardization<br />
or process-approach through quality management systems (QMS) certification have tended to pursue TQM as the subsequent<br />
stage in their quality journey. In addition, the study found that all seven TQM practices - leadership; strategy and planning;<br />
customer focus; information and analysis; people management; process management; and supplier involvement - were<br />
significantly higher in TQM firms than in non-TQM firms. Finally, the study found that tier 3 suppliers were less likely to<br />
implement TQM practices compared with higher tiers (1 and 2), except in supplier involvement. Originality/value: The study<br />
presents an insight into TQM constructs evolution in the ASEAN region, which has gained increased prominence and world<br />
impact as a result <strong>of</strong> international outsourcing. It therefore addresses a significant gap in the literature about how quality<br />
management is deployed in this important region <strong>of</strong> the world. © Emerald Group Publishing Limited.<br />
No.1101<br />
Author (s) : Punnakitikashem, P., Somsuk, N., Mclean, M.W., Laosirihongthong, T.<br />
Title : Linkage between continual improvement and knowledge-based view theory<br />
Source : Proceedings - <strong>2010</strong> IEEE 17th <strong>International</strong> Conference on Industrial Engineering and Engineering Management, IE and EM<strong>2010</strong>,<br />
<strong>2010</strong>, Article number 5646081, Pages 1689-1694<br />
Document Type : Conference Paper<br />
Keywords : Continual improvement; Embedded operations learning; Knowledge creation; Knowledge-based view (KBV) theory<br />
<strong>Abstract</strong> : This paper reviews the literature and exhibits finding from case studies to provide a broad overview <strong>of</strong> learning and<br />
knowledge creation in the context <strong>of</strong> continual improvement. First, we describe Knowledge-Based View (KBV) theory and its<br />
application in the context <strong>of</strong> Continual Improvement (CI) initiatives such as Quality Control Circles (QCC), Kaizen, Business<br />
Process Engineering, and Six Sigma from literature. We then propose an integrated view <strong>of</strong> knowledge creation using<br />
Nanoka's theory <strong>of</strong> knowledge creation to provide insights on how the CI initiatives are supported by this theory, which later<br />
lead to improve the operational and organization outcomes. The case studies <strong>of</strong> three manufacturing companies, which<br />
implemented CI program and multi-certified management systems, are then presented to illustrate how KBV can be applied<br />
to support CI initiative. This embedding operations learning is a complementary to the organization's knowledge management<br />
system facilitating sustainable CI projects. © <strong>2010</strong> IEEE.<br />
No.1102<br />
Author (s) : Punpanich, W., Boon-Yasidhi, V., Chokephaibulkit, K., Prasitsuebsai, W., Chantbuddhiwet, U., Leowsrisook, P., Hays, R.D., Detels,<br />
R.<br />
Title : Health-related Quality <strong>of</strong> Life <strong>of</strong> Thai children with HIV infection: A comparison <strong>of</strong> the Thai Quality <strong>of</strong> Life in Children (ThQLC)<br />
with the Pediatric Quality <strong>of</strong> Life Inventory version 4.0 (PedsQL 4.0) Generic Core Scales<br />
Source : Quality <strong>of</strong> Life Research, Volume 19, Issue 10, December <strong>2010</strong>, Pages 1509-1516<br />
Document Type : Article<br />
401
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : HAART; HIV infection; HRQoL; Pediatric; PedsQL 4.0; QoL; ThQLC<br />
<strong>Abstract</strong> : Purpose: The purpose <strong>of</strong> this study was to evaluate the reliability and validity <strong>of</strong> the Thai Quality <strong>of</strong> Life in Children (ThQLC)<br />
and compare it with the Pediatric Quality <strong>of</strong> Life Inventory (PedsQL 4.0) in a sample <strong>of</strong> children receiving long-term HIV care<br />
in Thailand. Methods: The ThQLC and the PedsQL 4.0 were administered to 292 children with HIV infection aged 8-16 years.<br />
Clinical parameters such as the current viral load, CD4 percent, and clinical staging were obtained by medical record review.<br />
Results: Three out <strong>of</strong> five ThQLC scales and three out <strong>of</strong> four PedsQL 4.0 scales had acceptable internal consistency<br />
reliability (i.e., Cronbach's alpha >0.70). Cronbach's alpha values <strong>of</strong> each scale ranged from 0.52 to 0.75 and 0.57 to 0.75 for<br />
the ThQLC and the PedsQL 4.0, respectively. Corresponding scales (physical functioning, emotional well-being, social<br />
functioning, and school functioning) <strong>of</strong> the ThQLC and the PedsQL 4.0 correlated substantially with one another (r = 0.47,<br />
0.67, 0.59 and 0.56, respectively). Both ThQLC and PedsQL 4.0 overall scores significantly correlated with the child's selfrated<br />
severity <strong>of</strong> the illness (r = -0.23 for the ThQLC and -0.28 for the PedsQL 4.0) and the caregiver's rated overall quality <strong>of</strong><br />
life (r = 0.07 for the ThQLC and 0.13 for the PedsQL 4.0). The overall score <strong>of</strong> the ThQLC correlated with clinical and<br />
immunologic categories <strong>of</strong> the United State-Centers for Disease Control and Prevention (US-CDC) classification system (r = -<br />
0.12), while the overall score <strong>of</strong> the PedsQL 4.0 significantly correlated with the number <strong>of</strong> disability days (r = -0.12) and<br />
CD4 percent (r = -0.15). However, the overall score from both instruments were not significantly different by clinical stages <strong>of</strong><br />
HIV disease. A multitrait-multimethod analysis results demonstrated that the average convergent validity and <strong>of</strong>f-diagonal<br />
correlations were 0.58 and 0.45, respectively. Discriminant validity was partially supported with 62% <strong>of</strong> validity diagonal<br />
correlations exceeding correlations between different domains (discriminant validity successes). The Hays-Hayashi MTMM<br />
quality index was 0.61. Multivariate regression analysis revealed that the ThQLC physical functioning scale provided unique<br />
information in predicting child self-rated severity <strong>of</strong> the illness and overall quality <strong>of</strong> life beyond that explained by the<br />
PedsQL 4.0 in Thai children with HIV infection. Conclusions: We found evidence in support <strong>of</strong> the reliability and validity <strong>of</strong><br />
the ThQLC and the PedsQL 4.0 for measuring the health-related quality <strong>of</strong> life <strong>of</strong> Thai children with HIV infection. © <strong>2010</strong><br />
The Author(s).<br />
No.1103<br />
Author (s) : Punsawad, Y., Aempedchr, S., Wongsawat, Y., Parnichkun, M.<br />
Title : EEG-based mental fatigue alarm system using weighted-frequency index<br />
Source : APSIPA ASC <strong>2010</strong> - Asia-Pacific Signal and Information Processing Association Annual Summit and Conference, <strong>2010</strong>, Pages 193-<br />
196<br />
Document Type : Conference Paper<br />
Keywords : Beta rhythms, Car accidents, Linear combinations, Mental fatigue, Prediction accuracy, Simulated driving; Alarm systems, Data<br />
processing, Interfaces (computer); Brain computer interface<br />
<strong>Abstract</strong> : Mental fatigue is another major cause <strong>of</strong> the serious car accidents. Ability to early predict the mental fatigue phenomenon is<br />
hence one <strong>of</strong> the challenging problems in brain-computer interface (BCI). In this paper, we propose the practical EEG-based<br />
mental fatigue alarm system including the weighted-frequency index <strong>of</strong> the linear combination among EEG theta, alpha and<br />
beta rhythms. The proposed system is tested with the simulated driving situations. By using only 1-channel EEG at the<br />
temporal area <strong>of</strong> the brain, more than 90% <strong>of</strong> prediction accuracies are reported compared to the opinion scores <strong>of</strong> the<br />
users.<br />
No.1104<br />
Author (s) : Punsawad, Y., Wongsawat, Y., Parnichkun, M.<br />
Title : Hybrid EEG-EOG brain-computer interface system for practical machine control<br />
Source : <strong>2010</strong> Annual <strong>International</strong> Conference <strong>of</strong> the IEEE Engineering in Medicine and Biology Society, EMBC'10, <strong>2010</strong>, Article number<br />
5626745, Pages 1360-1363<br />
Document Type : Conference Paper<br />
Keywords : Biosignals, Classification accuracy, Electro-oculogram, Hybrid framework, Machine controls, Practical issues; Computer control<br />
systems, Electroencephalography, Interfaces (computer), Optical sensors; Brain computer interface<br />
<strong>Abstract</strong> : Practical issues such as accuracy with various subjects, number <strong>of</strong> sensors, and time for training are important problems <strong>of</strong><br />
existing brain-computer interface (BCI) systems. In this paper, we propose a hybrid framework for the BCI system that can<br />
make machine control more practical. The electrooculogram (EOG) is employed to control the machine in the left and right<br />
directions while the electroencephalogram (EEG) is employed to control the forword, no action, and complete stop motions<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>of</strong> the machine. By using only 2-channel biosignals, the average classification accuracy <strong>of</strong> more than 95% can be achieved. ©<br />
<strong>2010</strong> IEEE.<br />
No.1105<br />
Author (s) : Punurai, W., Tongpool, W., Saardwong, W.<br />
Title : An extended nonlinear-based method for optimum cutting pattern generation <strong>of</strong> membrane structures<br />
Source : AIP Conference Proceedings, Volume 1233, Issue PART 1, <strong>2010</strong>, Pages 970-975<br />
Document Type : Conference Paper<br />
Keywords : cutting pattern; finite element method; genetic algorithm; membrane structures<br />
<strong>Abstract</strong> : The cutting pattern for membrane structures obtained by using the geodesic line method or flattening technique does not<br />
allow the actual stresses in the structures to be specified. As a result, the occurrence <strong>of</strong> surface wrinkles may form under<br />
service loading. In this paper, a method for determining an optimum cutting pattern which accounts for the designed stresses<br />
<strong>of</strong> the membrane structures is presented. Using the displacements <strong>of</strong> the 3-D surface as the key variables, the method<br />
proposed utilizes a nonlinear analysis technique based upon the second Piola-Kirchh<strong>of</strong>f stress tensor, Green strain tensor and<br />
Saint Venant-Kirchh<strong>of</strong>f hyperelastic material. The genetic algorithm is also adopted to solve the optimization problem. To<br />
verify the validation <strong>of</strong> the existing method, a cone shape membrane structure was analyzed. By using the proposed method,<br />
the deviation <strong>of</strong> the actual membrane stresses from the assumed design stresses could be reduced. © <strong>2010</strong> American<br />
Institute <strong>of</strong> Physics.<br />
No.1106<br />
Author (s) : Pusoonthornthum, R., Pusoonthornthum, P., Krishnamra, N.<br />
Title : Calcium-phosphorus homeostasis and changes in parathyroid hormone secretion in cats with various stages <strong>of</strong> spontaneous<br />
chronic renal failure<br />
Source : Comparative Clinical Pathology, Volume 19, Issue 3, June <strong>2010</strong>, Pages 287-293<br />
Document Type : Article<br />
Keywords : Cats; Chronic renal failure; Parathyroid hormone<br />
<strong>Abstract</strong> : Feline chronic renal failure was recognized with increased frequency in Maine coon, Abyssinian, Siamese, Russian blue, and<br />
Burmese cats. The objective <strong>of</strong> this study was to investigate the relationship between parathyroid hormone (PTH) level,<br />
calcium, and phosphorus homeostasis and the development <strong>of</strong> various stages <strong>of</strong> the naturally occurring chronic renal failure<br />
(CRF) in cats. Thirty-two CRF cats without history <strong>of</strong> receiving special diet for renal diseases that were presented to the Small<br />
Animal Hospital, Faculty <strong>of</strong> Veterinary Science, Chulalongkorn <strong>University</strong> were studied. Nineteen CRF cats were followed<br />
prospectively for 60 days and divided into two groups: uremic group (11 cats) and end-stage group (eight cats). The control<br />
group (13 cats) were normal cats, which were brought for vaccination at the same hospital within the same period. CRF cats<br />
with blood urea nitrogen concentrations <strong>of</strong> more than 50 mg/dl, serum creatinine level <strong>of</strong> more than 2. 1 mg/dl, and urine<br />
specific gravity <strong>of</strong> between 1. 008 and 1. 014 were included into the study. Completed blood count, blood chemistry,<br />
electrolytes, including sodium, potassium, total calcium, and phosphorus, and PTH levels were measured on days 0, 14, 30,<br />
and 60 after the first diagnosis. The results showed that cats with CRF had significantly lower red blood cells, hemoglobin,<br />
and pack cell volume than control cats (p < 0. 01) on days 0, 14, 30, and 60. PTH levels on first day <strong>of</strong> diagnosis were 50. 51<br />
± 19. 65, 79. 41 ± 28. 12, and 183. 37 ± 50. 12 pg/ml in controls, uremic, and end-stage groups, respectively. Cats in end-stage<br />
group had significantly increased levels <strong>of</strong> PTH when compared to control (p < 0. 01) and uremic groups (p < 0. 05) on days 0,<br />
14, and 30. Serum phosphorus levels also increased significantly in end-stage group (p < 0. 001), indicating the presence <strong>of</strong><br />
renal secondary hyperparathyroidism. This study reveals that PTH level is significantly increased in end-stage CRF cats who did<br />
not received special diet for renal diseases. The development <strong>of</strong> renal secondary hyperparathyroidism in end-stage CRF cats<br />
significantly decreased its survival rate. © 2009 Springer-Verlag London Limited.<br />
No.1107<br />
Author (s) : Putaporntip, C., Udomsangpetch, R., Pattanawong, U., Cui, L., Jongwutiwes, S.<br />
Title : Genetic diversity <strong>of</strong> the Plasmodium vivax merozoite surface protein-5 locus from diverse geographic origins<br />
Source : Gene, Volume 456, Issue 1-2, May <strong>2010</strong>, Pages 24-35<br />
Document Type : Article<br />
Keywords : Genetic differentiation; Genetic diversity; Malaria; Merozoite surface protein-5; Natural selection; Plasmodium knowlesi;<br />
Plasmodium vivax<br />
403
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : Plasmodium vivax merozoite surface protein-5 (PvMsp-5), a potential vaccine candidate, is encoded by a two-exon single<br />
copy gene. We have conducted a comprehensive analysis <strong>of</strong> PvMsp-5 by sequencing the entire gene <strong>of</strong> four parasite<br />
populations from northwestern Thailand (n=73), southern Thailand (n=53), Indonesia (n=25) and Brazil (n=24), and five<br />
isolates from other endemic areas. Results reveal that exon I exhibits a significantly higher level <strong>of</strong> nucleotide diversity at<br />
both synonymous and nonsynonymous sites than exon II (p<0.01). Neutrality tests based on both intraspecific and<br />
interspecific nucleotide polymorphism have detected a signature <strong>of</strong> positive selection in exon I <strong>of</strong> all populations while<br />
substitutions in exon II mainly followed neutral expectation except that three residues in exon II <strong>of</strong> northwestern Thailand<br />
population appear to be positively selected using the Bayes Empirical Bayes method. Short imperfect repeats were identified<br />
in exon I at an equivalent region to its orthologue in P. knowlesi, supporting their close genetic relatedness. Significant levels<br />
<strong>of</strong> population subdivision were detected among most populations including those between northwestern and southern<br />
Thailand (p<10-5), implying absent or minimal gene flow between these populations. Importantly, evidences for intragenic<br />
recombination in PvMsp-5 were found in most populations except that from southern Thailand in which haplotype diversity<br />
and nucleotide diversity were exceptionally low. Results from Fu and Li's D, F and D and F tests suggested that PvMsp-5 <strong>of</strong><br />
most P. vivax populations have been maintained by balancing selection whereas southern Thailand population could have<br />
gone through recent bottleneck events. These findings are concordant with a substantial reduction in the number <strong>of</strong> P. vivax<br />
cases in southern Thailand during the past decade, followed by a very recent population expansion. Therefore, spatiotemporal<br />
monitoring <strong>of</strong> parasite population genetics provides important implications for disease control. © <strong>2010</strong> Elsevier B.V.<br />
No.1108<br />
Author (s) : Puthanakit, T., Aurpibul, L., Yoksan, S., Sirisanthana, T., Sirisanthana Virat, V.<br />
Title : A 3-year follow-up <strong>of</strong> antibody response in HIV-infected children with immune recovery vaccinated with inactivated Japanese<br />
encephalitis vaccine<br />
Source : Vaccine, Volume 28, Issue 36, August <strong>2010</strong>, Pages 5900-5902<br />
Document Type : Article<br />
Keywords : Human immunodeficiency virus (HIV); Japanese encephalitis virus; Protective antibody<br />
<strong>Abstract</strong> : Among HIV-infected children who had immune recovery after received antiretroviral therapy (ART), good responses to<br />
revaccination with childhood vaccines have been observed. However, the rate <strong>of</strong> long-term persistence <strong>of</strong> antibody response<br />
remains unknown. The objective <strong>of</strong> this study is to determine whether HIV-infected children still have protective antibody<br />
against Japanese encephalitis virus (JE) 3 years after receiving revaccination with two doses <strong>of</strong> inactivated JE vaccine. Plasma<br />
JE neutralizing antibody titer was determined by a plaque reduction neutralization assay. An antibody titer <strong>of</strong> more than 1:10<br />
was defined as being protective. Fifty HIV-infected children with a mean age <strong>of</strong> 10.3 years (SD 2.2) and mean CD4 percentage<br />
<strong>of</strong> 25 (SD 5) were revaccinated with two doses <strong>of</strong> inactivated JE vaccine. Forty-three children had been followed-up for 3<br />
years. The JE neutralizing antibody at 1 month and 3 years after revaccination were detected among 38 (88%) and 35 (81%)<br />
children, respectively. The geometric means titer significantly dropped from <strong>of</strong> 306 (min 13-max 163,617) to 106 (min 11-max<br />
4645). This data show that the majority <strong>of</strong> HIV-infected children had persistent antibody 3 years after revaccination. JE<br />
revaccination in HIV-infected children with immune recovery after ART should be carried out in endemic areas. © <strong>2010</strong><br />
Elsevier Ltd.<br />
No.1109<br />
Author (s) : Puthanakit, T., Chokephaibulkit, K., Suntarattiwong, P., Gorowara, M., Vanprapar, N., Leawsrisuk, P., Suwanlerk, T., Boonrak, P.,<br />
Ruxrungtham, K.<br />
Title : Therapeutic drug monitoring <strong>of</strong> lopinavir in human immunodeficiency virus-infected children receiving adult tablets<br />
Source : Pediatric Infectious Disease Journal, Volume 29, Issue 1, January <strong>2010</strong>, Pages 79-82<br />
Document Type : Article<br />
Keywords : Children; Generic drug tablets; HIV; Lopinavir; Therapeutic drug monitoring<br />
<strong>Abstract</strong> : Because <strong>of</strong> the lack <strong>of</strong> a lopinavir/ritonavir (LPV/r) pediatric formulation, 54 HIV-infected children were given generic LPV/r<br />
adult tablets. Of 54 children, 21 took cut pills to get the appropriate dose. The median (interquartile range) LPV trough serum<br />
concentration (Ctrough) was 6.7 (5.0-9.9) mg/L. All the children had Ctrough >1.0 mg/L and 96% had values >4.0 mg/L. LPV/r<br />
adult tablets can be used in children when it is necessary. © 2009 by Lippincott Williams & Wilkins.<br />
404
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.1110<br />
Author (s) : Puthanakit, T., Jourdain, G., Hongsiriwon, S., Suntarattiwong, P., Chokephaibulkit, K., Sirisanthana, V., Kosalaraksa, P., Petdachai,<br />
W., Hansudewechakul, R., Siangphoe, U., Suwanlerk, T., Ananworanich, J.<br />
Title : HIV-1 drug resistance mutations in children after failure <strong>of</strong> first-line nonnucleoside reverse transcriptase inhibitor-based<br />
antiretroviral therapy<br />
Source : HIV Medicine, Volume 11, Issue 9, October <strong>2010</strong>, Pages 565-572<br />
Document Type : Article<br />
Keywords : Children; HIV; Nonnucleoside reverse transcriptase inhibitor; Resistance mutations; Treatment failure<br />
<strong>Abstract</strong> : Objectives: The aim <strong>of</strong> the study was to assess the prevalence, predictors and patterns <strong>of</strong> genotypic resistance mutations in<br />
children after failure <strong>of</strong> World Health Organization-recommended initial nonnucleoside reverse transcriptase inhibitor (NNRTI)based<br />
treatment regimens. Methods: We carried out a multicentre retrospective study <strong>of</strong> genotyping tests performed for all<br />
HIV-infected children at eight paediatric centres in Thailand who experienced failure <strong>of</strong> NNRTI therapy at a time when<br />
virological monitoring was not routinely available. Results: One hundred and twenty children were included in the study.<br />
Their median age (interquartile range) was 9.1 (6.8-11.0) years, the median duration <strong>of</strong> their NNRTI regimens was 23.7 (15.7-<br />
32.6) months, their median CD4 percentage was 12% (4-20%), and their median plasma HIV RNA at the time <strong>of</strong> genotype<br />
testing was 4.8 (4.3-5.2) log10 HIV-1 RNA copies/mL. The nucleoside reverse transcriptase inhibitor (NRTI) resistance mutations<br />
found were as follows: 85% <strong>of</strong> the children had M184V/I, 23% had at least four thymidine analogue mutations, 12% had the<br />
Q151M complex, 5% had K65R, and 1% had the 69 insertion. Ninety-eight per cent <strong>of</strong> the children had at least one NNRTI<br />
resistance mutation, and 48% had etravirine mutation-weighted scores ≥4. CD4 percentage <15% prior to switching<br />
regimens [odds ratio (OR) 5.49; 95% confidence interval (CI) 2.02-14.93] and plasma HIV RNA>5 log10 copies/mL (OR 2.46;<br />
95% CI 1.04-5.82) were independent predictors <strong>of</strong> at least four thymidine analogue mutations, the Q151M complex or the 69<br />
insertion. Conclusions: In settings without routine viral load monitoring, second-line antiretroviral therapy regimens should be<br />
designed assuming that clinical or immunological failure is associated with high rates <strong>of</strong> multi-NRTI resistance and NNRTI<br />
resistance, including resistance to etravirine. © <strong>2010</strong> British HIV Association.<br />
No.1111<br />
Author (s) : Puthanakit, T., Tangsathapornpong, A., Ananworanich, J., Wongsawat, J., Suntrattiwong, P., Wittawatmongkol, O., Mekmullica, J.,<br />
Waidab, W., Bhakeecheep, S., Chokephaibulkit, K.<br />
Title : Thai national guidelines for the use <strong>of</strong> antiretroviral therapy in pediatric HIV infection in <strong>2010</strong><br />
Source : Asian Biomedicine, Volume 4, Issue 4, August <strong>2010</strong>, Pages 505-513<br />
Document Type : Review<br />
Keywords : HIV; Pediatrics; Thai guidelines<br />
<strong>Abstract</strong> : With better knowledge and availability <strong>of</strong> antiretroviral treatments, the Thai National HIV Guidelines Working Group has issued<br />
treatment guidelines for children in Thailand in March <strong>2010</strong>. The most important aspects <strong>of</strong> these new guidelines are detailed<br />
below. ART should be initiated in infants less than 12 months <strong>of</strong> age at any CD4 level regardless <strong>of</strong> symptoms and in all<br />
children at CDC clinical stage B and C or WHO clinical stages 3 and 4. For children with no or mild symptoms consider CD4guided<br />
thresholds <strong>of</strong> CD4 <25% (children aged one to five years) or CD4 <350 cells/mm3 (children 5 years or older). The<br />
preferred first-line regimen in children aged < 3 years is AZT+3TC+NVP. For children ≥3 years <strong>of</strong> age the preferred regimen is<br />
AZT+3TC+EFV. If an infant has previously been exposed to NVP perinatally, use AZT+3TC+LPV/r as empirical first regimen. In<br />
adolescents, consider TDF+3TC+EFV. The preferred ARV treatment in children who failed first line regimens <strong>of</strong> 2NRTI+NNRTI<br />
(Salvage treatment) comprises 2NRTI (guided by genotype) +LPV/r, and an alternative regimen is 2NRTI (guided by genotype)<br />
+ATV/ r (use in cases with dyslipidemia who are six years or older). In cases with extensive NRTI resistance with no effective<br />
NRTI option available, double boosted PI with LPV/r+SQV or LPV/r+IDV can be considered. Consultation with an expert is<br />
recommended. Laboratory monitoring is recommended for CD4 and every six months. Viral load at least at 6 and 12 months<br />
after initiation or change <strong>of</strong> regimen, then yearly thereafter. More frequent viral load monitoring is advised for cases with<br />
unsuccessful virologic response, infants, children with imperfect adherence, or those using <strong>of</strong> third line regimens. Toxicity<br />
monitoring depends on the drug received, at least every six months, and more <strong>of</strong>ten as clinically indicated. These include,<br />
but are not limited to, complete blood count, renal function tests, liver function tests, urinanalysis, and lipid pr<strong>of</strong>iles.<br />
Therapeutic drug monitoring is recommended in cases that have ARV-related toxicity, receiving non-standard dosing or<br />
regimens, using double boosted PI, and in those with renal or hepatic impairment.<br />
405
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.1112<br />
Author (s) : Puthawibool, T., Senapin, S., Flegel, T.W., Kiatpathomchai Wansika, W.<br />
Title : Rapid and sensitive detection <strong>of</strong> Macrobrachium rosenbergii nodavirus in giant freshwater prawns by reverse transcription<br />
loop-mediated isothermal amplification combined with a lateral flow dipstick<br />
Source : Molecular and Cellular Probes, Volume 24, Issue 5, October <strong>2010</strong>, Pages 244-249<br />
Document Type : Review<br />
Keywords : Giant freshwater prawn; LAMP; Lateral flow dipstick; LFD; Loop-mediated isothermal amplification; Macrobrachium<br />
rosenbergii; Macrobrachium rosenbergii nodavirus; MrNV; PCR,; White tail disease<br />
<strong>Abstract</strong> : Loop-mediated isothermal amplification (LAMP) allows rapid amplification <strong>of</strong> nucleic acids under isothermal conditions. It can<br />
be combined with a chromatographic lateral flow dipstick (LFD) for much more efficient, field-friendly detection <strong>of</strong> MrNV. In<br />
this work, RT-LAMP was performed at 65 °C for 40 min, followed by 5 min for hybridization with an FITC-labeled DNA probe<br />
and 5 min for LFD resulted in visualization <strong>of</strong> DNA amplicons trapped at the LFD test line. Thus, total assay time, including 10<br />
min for rapid RNA extraction was approximately 60 min. In addition to advantages <strong>of</strong> short assay time, confirmation <strong>of</strong><br />
amplicon identity by hybridization and elimination <strong>of</strong> electrophoresis with carcinogenic ethidium bromide, the RT-LAMP-LFD<br />
was more sensitive than an existing RT-PCR method for detection <strong>of</strong> MrNV. The RT-LAMP-LFD method gave negative test<br />
results with nucleic acid extracts from normal shrimp and from shrimp infected with other viruses including DNA viruses<br />
[PstDNV (IHHNV), PemoNPV (MBV), PmDNV (HPV), WSSV] and RNA viruses (TSV, IMNV, YHV/GAV). © <strong>2010</strong> Elsevier Ltd.<br />
No.1113<br />
Author (s) : Putri, S.P., Kinoshita, H., Ihara, F., Igarashi, Y., Nihira, T.<br />
Title : Ophiosetin, a new tetramic acid derivative from the mycopathogenic fungus Elaphocordyceps ophioglossoides<br />
Source : Journal <strong>of</strong> Antibiotics, Volume 63, Issue 4, April <strong>2010</strong>, Pages 195-198<br />
Document Type : Article<br />
Keywords : Elaphocordyceps ophioglossoides; Mycopathogenic fungus; Ophiosetin; Tetramic acid<br />
<strong>Abstract</strong> : –<br />
No.1114<br />
Author (s) : Putwattana, N., Kruatrachue, M., Pokethitiyook, P., Chaiyarat, R.<br />
Title : Immobilization <strong>of</strong> cadmium in soil by cow manure and silicate fertilizer, and reduced accumulation <strong>of</strong> cadmium in sweet basil<br />
(Ocimum basilicum)<br />
Source : ScienceAsia, Volume 36, Issue 4, December <strong>2010</strong>, Pages 349-354<br />
Document Type : Article<br />
Keywords : Cadmium accumulation; Dry biomass production; Pot study; Soil amendments<br />
<strong>Abstract</strong> : This study was conducted to determine the effects <strong>of</strong> organic and inorganic soil additives in reducing Cd concentration in the<br />
edible parts <strong>of</strong> plants. A pot study was performed by growing Ocimum basilicum (sweet basil) in Cd contaminated soil (20<br />
mg/kg Cd) and soils amended with cow manure and silicate fertilizer for 3 months. The results showed an increase in dry<br />
biomass production by factors <strong>of</strong> 4.7 and 1.7 in plants grown in soil supplemented with cow manure (20% w/w) and silicate<br />
fertilizer (20% w/w), respectively. Shoot Cd accumulation doubled in plants grown in cow manure treated soil. In contrast,<br />
the silicate fertilizer resulted in a 3-fold decrease in leaf Cd accumulation. The results <strong>of</strong> this investigation demonstrated the<br />
potential <strong>of</strong> silicate fertilizer in reducing Cd transport from roots to shoots, resulting in decreased Cd concentration in the<br />
edible parts <strong>of</strong> plants.<br />
No.1115<br />
Author (s) : Rachadech, W., Navacharoen, A., Ruangsit, W., Pongtharangkul, T., Vangnai, A.S.<br />
Title : An organic solvent-, detergent-, and thermostable alkaline protease from the mesophilic, organic solvent-tolerant Bacillus<br />
licheniformis 3C51<br />
Source : Microbiology, Volume 79, Issue 5, <strong>2010</strong>, Pages 620-629<br />
Document Type : Article<br />
Keywords : alkaline protease; Bacillus licheniformis; organic-solvent tolerant bacterium; thermo-, organic-, solvent-, detergentstable<br />
protease<br />
<strong>Abstract</strong> : Bacillus licheniformis 3C5, isolated as mesophilic bacterium, exhibited tolerance towards a wide range <strong>of</strong> non-polar and polar<br />
organic solvents at 45°C. It produced an extracellular organic solvent-stable protease with an apparent molecular mass <strong>of</strong><br />
406
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
approximately 32 kDa. The inhibitory effect <strong>of</strong> PMSF and EDTA suggested it is likely to be an alkaline serine protease. The<br />
protease was active over a broad range <strong>of</strong> temperatures (45-70°C) and pH (8-10) range with an optimum activity at pH 10 and<br />
65°C. It was comparatively stable in the presence <strong>of</strong> a relatively high concentration (35% (v/v)) <strong>of</strong> organic solvents and various<br />
types <strong>of</strong> detergents even at a relatively high temperature (45°C). The protease production by B. licheniformis 3C5 was growthdependent.<br />
The optimization <strong>of</strong> carbon and nitrogen sources for cell growth and protease production revealed that yeast<br />
extract was an important medium component to support both cell growth and the protease production. The overall<br />
properties <strong>of</strong> the protease produced by B. licheniformis 3C5 suggested that this thermo-stable, solvent-stable, detergentstable<br />
alkaline protease is a promising potential biocatalyst for industrial and environmental applications. © <strong>2010</strong> Pleiades<br />
Publishing, Ltd.<br />
No.1116<br />
Author (s) : Rahman, W., Chotivanich, K., Silamut, K., Tanomsing, N., Hossain, A., Faiz, M.A., Dondorp, A.M., Maude, R.J.<br />
Title : Plasmodium malariae in Bangladesh<br />
Source : Transactions <strong>of</strong> the Royal Society <strong>of</strong> Tropical Medicine and Hygiene, Volume 104, Issue 1, January <strong>2010</strong>, Pages 78-80<br />
Document Type : Article<br />
Keywords : Bangladesh; Malaria; PCR; Plasmodium malariae; Rosetting; severe<br />
<strong>Abstract</strong> : We describe a 32-year-old Bangladeshi male presenting with severe malaria caused by a mono-infection with Plasmodium<br />
malariae. Rosetting <strong>of</strong> infected and uninfected erythrocytes, a putative virulence factor in falciparum malaria, was observed in<br />
the blood slide. Severe disease caused by P. malariae is extremely rare. The patient made a rapid recovery with intravenous<br />
quinine treatment. © 2009 Royal Society <strong>of</strong> Tropical Medicine and Hygiene.<br />
No.1117<br />
Author (s) : Rakbud, J., Chaisuriya, P.<br />
Title : Schatten classes <strong>of</strong> matrices in a generalized B(l2)<br />
Source : Journal <strong>of</strong> the Korean Mathematical Society, Volume 47, Issue 1, <strong>2010</strong>, Pages 29-40<br />
Document Type : Article<br />
Keywords : Compact operator; Schatten p-class; Singular values<br />
<strong>Abstract</strong> : In this paper, we study a generalization <strong>of</strong> the Banach space B(l2) <strong>of</strong> all bounded linear operators on l2. Over this space, we<br />
present some reasonable ways to define Schatten-type classes which are general- izations <strong>of</strong> the classical Schatten classes <strong>of</strong><br />
compact operators on l2. © <strong>2010</strong> The Korean Mathematical Society.<br />
No.1118<br />
Author (s) : Rakkhong, K.; Kamchaisatian, W.; Vilaiyuk, S.; Benjaponpitak, S.<br />
Title : Clinical Manifestation and Natural Course <strong>of</strong> Cow's Milk Allergy in Ramathibodi Hospital, Bangkok<br />
Source : JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, Volume: 125, Issue: 2, Supplement: 1, Pages: AB25-AB25, Published: FEB<br />
<strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1119<br />
Author (s) : Rakthong, P., Intaramat, A., Ratanabanangkoon, K.<br />
Title : Luminol encapsulated liposome as a signal generator for the detection <strong>of</strong> specific antigen-antibody reactions and nucleotide<br />
hybridization<br />
Source : Analytical Sciences, Volume 26, Issue 7, July <strong>2010</strong>, Pages 767-772<br />
Document Type : Article<br />
Keywords : 1,2 distearoylphosphatidylethanolamine, 1,2-distearoylphosphatidylethanolamine, immobilized protein, liposome, luminol,<br />
macrogol derivative, oligonucleotide, phosphatidylethanolamine, streptavidin; antibody specificity, antigen antibody complex,<br />
antigen antibody reaction, article, biotinylation, chemistry, feasibility study, genetic procedures, genetics, immunology, light,<br />
methodology, nucleic acid hybridization, nucleotide sequence, radiation scattering, surface property; Antibody Specificity,<br />
Antigen-Antibody Complex, Antigen-Antibody Reactions, Base Sequence, Biosensing Techniques, Biotinylation, Feasibility<br />
407
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Studies, Immobilized Proteins, Light, Liposomes, Luminol, Nucleic Acid Hybridization, Oligonucleotides,<br />
Phosphatidylethanolamines, Polyethylene Glycols, Scattering, Radiation, Streptavidin, Surface Properties<br />
<strong>Abstract</strong> : Liposomes prepared with biotinylated phospholipids and luminol entrapped were shown to be <strong>of</strong> 187 nm in size, 59% <strong>of</strong><br />
which were unilamellar and with 43% luminol trapping efficiency. Liposome prepared from biotinylated phospholipids with a<br />
longer hydrophilic PEG2000 spacer, but not with the shorter hydrophobic caproyl one, bound efficiently and specifically with<br />
immobilized streptavidin in a microplate assay. The interactions <strong>of</strong> dinitrophenol and tobramycin with their respective<br />
antibodies, and the hybridization <strong>of</strong> 20-mers oligonucleotides were studied using the liposome as a signal generator. These<br />
reactions were shown to be specific with limits <strong>of</strong> detection <strong>of</strong> 0.58 μM, 0.96 μM and 18 nM, respectively. <strong>2010</strong> © The Japan<br />
Society for Analytical Chemistry.<br />
No.1120<br />
Author (s) : Rangkadilok, N.; Pholphana, N.; <strong>Mahidol</strong>, C.; Wongyai, W.; Saengsooksree, K.; Nookabkaew, S.; Satayavivad, J.<br />
Title : Variation <strong>of</strong> sesamin, sesamolin and tocopherols in sesame (Sesamum indicum L.) seeds and oil products in Thailand<br />
Source : FOOD CHEMISTRY, Volume: 122, Issue: 3, Pages: 724-730, DOI: 10.1016/j.foodchem.<strong>2010</strong>.03.044, Published: OCT 1 <strong>2010</strong><br />
Document Type : Article<br />
Keywords : Sesame seed; Sesamin; Sesamolin; Tocopherol; HPLC; Sesame oil<br />
<strong>Abstract</strong> : Sesame (Sesamum indicum L) seed and oil contain abundant lignans, including sesamin, sesamolin and lignan glycosides. The<br />
aim <strong>of</strong> the present study was to determine sesamin, sesamol in and tocopherol contents in sesame seed and oil available in<br />
Thailand. The results showed that there was a large variation <strong>of</strong> sesamin and sesamolin contents in products. The distribution<br />
plot <strong>of</strong> sesamin and sesamolin contents in seeds showed that the mean values <strong>of</strong> sesamin and sesamolin were 1.55 mg/g<br />
(SD= 1.63; range n.d.-7.23 mg/g) and 0.62 mg/g (SD = 0.48; range n.d.-2.25 mg/g), respectively. The range <strong>of</strong> total tocopherols<br />
<strong>of</strong> these sesame lines was 50.9-211 mu g/g seed. In commercial sesame oils, the ranges <strong>of</strong> sesamin and sesamolin were 0.93-<br />
2.89 mg/g oil and 0.30-0.74 mg/g oil, respectively, and tocopherol contents were 304-647 mu g oil. The study reveals the<br />
extensive variability in sesamin, sesamolin and tocopherol contents among sesame products. (C) <strong>2010</strong> Elsevier Ltd. All rights<br />
reserved.<br />
No.1121<br />
Author (s) : Ratanamart, J., Huggins, C.G., Shaw, J.A.M.<br />
Titl : Transgene expression in mononuclear muscle cells not infiltrating inflammatory cells following intramuscular plasmid gene<br />
electrotransfer<br />
Source : Journal <strong>of</strong> Gene Medicine, Volume 12, Issue 4, April <strong>2010</strong>, Pages 377-384<br />
Document Type : Article<br />
Keywords : Electroporation; Immune response; Inflammation; Muscle-targeted gene transfer; Plasmid<br />
<strong>Abstract</strong> : Background: In situ electroporation-assisted intramuscular plasmid DNA delivery <strong>of</strong>fers high efficiency for therapeutic protein<br />
replacement. Expression may be impaired by an immune response against the plasmid or transgenic protein. Expression <strong>of</strong><br />
the transgene in non-muscle cells may increase the immune response. Gene transfer efficiency and phenotypic identification<br />
<strong>of</strong> intramuscular transgene-expressing mononuclear cells was studied following electroporation-mediated plasmid delivery.<br />
Methods: Plasmids expressing β-galactosidase (pVR1012-βgal) or enhanced green fluorescent protein (eGFP) (pVR1012-eGFP)<br />
were electrotransferred into rat tibialis anterior muscles. Both transfection efficiency and the inflammatory response were<br />
determined in pVR1012-βgal-injected muscles by β-galactosidase and haematoxylin and eosin staining <strong>of</strong> muscles 7 days<br />
post-plasmid injection. Muscles injected with pVR1012-eGFP were stained for CD3, CD68 and desmin at 24 and 48 h postinjection<br />
to determine whether mononuclear cells expressing eGFP were <strong>of</strong> immune or myogenic origin. Results: With<br />
electroporation, β-galactosidase expression was significantly enhanced by up to ten-fold compared to plasmid injection<br />
without electroporation. A large area <strong>of</strong> regenerating muscle fibres and inflammatory cell infiltration was found in<br />
electroporated plasmid-injected muscle. No eGFP expression was found in CD3- or CD68-positive cells. Small mononuclear<br />
cells expressing eGFP showed negative staining for CD3 and CD68, but all stained positive for desmin. Conclusions: In situ<br />
electroporation enhanced transfection efficiency <strong>of</strong> plasmid DNA delivery into muscle. Alongside its advantage for improving<br />
gene transfer, electroporation led to an increased inflammatory response and muscle damage. Mononuclear cells in muscle<br />
were transfected with plasmid and expressed the transgene. These cells were <strong>of</strong> myogenic origin with no evidence <strong>of</strong><br />
transgene expression in infiltrating inflammatory cells. Copyright © <strong>2010</strong> John Wiley & Sons, Ltd.<br />
408
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.1122<br />
Author (s) : Ratanarapee, S., Nualyong, C.<br />
Title : Acute appendicitis as primary symptom <strong>of</strong> prostatic adenocarcinoma: Report <strong>of</strong> a case<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 11, November <strong>2010</strong>, Pages 1327-1331<br />
Document Type : Article<br />
Keywords : Appendicitis; Metastatic prostatic carcinoma; Prostatic adenocarcinoma<br />
<strong>Abstract</strong> : The most common disease <strong>of</strong> appendix is acute appendicitis. There have been at least 20 case reports <strong>of</strong> metastasisinduced<br />
appendicitis in English literature. The authors reported a 62-year-old Thai man who underwent appendectomy due to<br />
classical symptoms and signs <strong>of</strong> acute appendicitis and was subsequently referred to Siriraj Hospital for proper management<br />
after a pathological report <strong>of</strong> metastatic adenocarcinoma to the appendix. By morphology, the primary site was thought to be<br />
the prostate gland. Confirmation was done based on serum PSA study, transrectal ultrasound-guided core needle biopsy, and<br />
review <strong>of</strong> the material from Hua Hin Hospital. Orchidectomy was performed with satisfactory outcome. The patient was doing<br />
well for two years before upper gastrointestinal hemorrhage and obstructive jaundice caused by another aggressive tumor<br />
developed. He died <strong>of</strong> the second tumor associated with subsequent renal failure in spite <strong>of</strong> good response to orchidectomy.<br />
No.1123<br />
Author (s) : Ratanarapee, S., Uiprasertkul, M., Pradniwat, K., Soontrapa, S.<br />
Title : Villous adenoma <strong>of</strong> the urinary bladder: A case report<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 11, November <strong>2010</strong>, Pages 1336-1339<br />
Document Type : Article<br />
Keywords : Bladder adenoma; Cystitis glandularis; Villous adenoma<br />
<strong>Abstract</strong> : Villous adenomas <strong>of</strong> the urinary tract are rare, in contrast to urothelial neoplasms. Most reports were scattered individual<br />
cases. Only two case series <strong>of</strong> this entity have been published. The histopathology is identical to that <strong>of</strong> the much more<br />
common villous adenoma <strong>of</strong> the gastrointestinal tract. The authors reported a case <strong>of</strong> urinary bladder villous adenoma in a<br />
41-year-old Thai patient who complained <strong>of</strong> hematuria for one day without any other symptom. Cystoscopic examination<br />
revealed a papillary growth at the bladder neck associated with marked degree <strong>of</strong> bullous edema and bilateral mild<br />
hydroureters. The clinical diagnosis was urothelial carcinoma. Transurethral resection was performed. Histologic examination<br />
revealed typical features <strong>of</strong> villous adenoma. The tumor showed identical immunohistochemical pr<strong>of</strong>ile to colonic villous<br />
adenoma. The patient has been well for more than a year after tumor removal.<br />
No.1124<br />
Author (s) : Ratanarat, R., Promsin, P., Srivijitkamol, A., Leemingsawat, C., Permpikul, C.<br />
Title : Diagnosis <strong>of</strong> corticosteroid insufficiency in Thai patients with septic shock.<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand = Chotmaihet thangphaet, Volume 93 Suppl 1, January <strong>2010</strong>, Pages S187-195<br />
Document Type : Article<br />
Keywords : corticotropin, diagnostic agent, hydrocortisone, vasoconstrictor agent; adrenal<br />
insufficiency, adult, aged, article, blood, endocrine function test, female, fluid therapy, follow up, human, hypophysis adrenal<br />
system, hypothalamus hypophysis system, male, methodology, middle aged, mortality, pathophysiology, prospective<br />
study, sensitivity and specificity, septic shock, Thailand; Adrenal Cortex Function Tests, Adrenal<br />
Insufficiency, Adrenocorticotropic Hormone, Adult, Aged, Female, Fluid Therapy, Follow-Up Studies, Hospital<br />
Mortality, Humans, Hydrocortisone, Hypothalamo-Hypophyseal System, Male, Middle Aged, Pituitary-Adrenal<br />
System, Prospective Studies, Sensitivity and Specificity, Shock, Septic, Thailand, Vasoconstrictor Agents<br />
<strong>Abstract</strong> : BACKGROUND: The reported incidence <strong>of</strong> critical illness-related corticosteroid insufficiency (CIRCI) varies widely, depending on<br />
the patient population studied and the diagnostic criteria used. Surviving Sepsis Campaign guidelines suggest that<br />
corticosteroid therapy should be considered for adult septic shock when hypotension responds poorly to adequate fluid<br />
resuscitation and vasopressors, regardless <strong>of</strong> any results <strong>of</strong> diagnostic tests. However, steroid treatment may be associated<br />
with an increase risk <strong>of</strong> infection. This study aims to identify the best diagnostic tool for predicting responsiveness to<br />
corticosteroid therapy in Thai septic shock patients with poorly responsive to fluid resuscitation and vasopressors. MATERIAL<br />
AND METHOD: Twenty-nine septic shock patients who were poorly responsive to fluid therapy and vasopressors were studied.<br />
A baseline serum total cortisol was measured in all patients and then 250 mcg corticotropin was injected to patients. Cortisol<br />
level was obtained 30 and 60 minutes after injection. All patients were given hydrocortisone (100 mg i.v., then 200 mg i.v. in<br />
24 hrs for at least 5 days). Patients were considered steroid responsive if vasopressor agent could be discontinued within 48<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
hrs after the first dose <strong>of</strong> hydrocortisone. RESULTS: Hospital mortality was 62% in which 45% <strong>of</strong> the patients were steroid<br />
responsive. Baseline serum cortisol was 27.6 +/- 11.4 microg/dl in the steroid-responsive patients compared with 40 +/- 16.9<br />
microg/dl in the steroid-nonresponsive patients (p = 0.03). The area under the ROC curves for predicting steroid<br />
responsiveness was 0.72 for baseline cortisol level. Serum cortisol level <strong>of</strong> 35 microg/dl or less was the most accurate<br />
diagnostic threshold to determine hemodynamic response to hydrocortisone treatment (p = 0.04). Using baseline cortisol<br />
level <strong>of</strong> < or = 35 microg/dl to diagnose adrenal insufficiency, the sensitivity was 85%, the specificity was 62% and the<br />
accuracy was 72%. A use <strong>of</strong> (delta cortisol) showed sensitivity <strong>of</strong> 50%, specificity <strong>of</strong> 30% and accuracy <strong>of</strong> 41%. CONCLUSION:<br />
Baseline cortisol level < or = 35 microg/dl is a useful diagnostic threshold for diagnosis <strong>of</strong> steroid responsiveness in Thai<br />
patients with septic shock and ACTH stimulation test should not be used.<br />
No.1125<br />
Author (s) : Rattanachitthawat, S., Suwannalert, P., Riengrojpitak, S., Chaiyasut, C., Pantuwatana, S.<br />
Title : Phenolic content and antioxidant activities in red unpolished Thai rice prevents oxidative stress in rats<br />
Source : Journal <strong>of</strong> Medicinal Plant Research, Volume 4, Issue 9, May <strong>2010</strong>, Pages 796-801<br />
Document Type : Article<br />
Keywords : Antioxidant; Oxidative stress; Phenolic compounds; Unpolished Thai rice<br />
<strong>Abstract</strong> : Radicals cause cellular damage and eventually progress to chronic diseases. Phenolic compounds play a crucial role in<br />
radicals scavenging. In this study, we investigated total anti-oxidant activities, total phenolic content and pr<strong>of</strong>iles in color<br />
strains <strong>of</strong> unpolished Thai rice. The level <strong>of</strong> malondialdehyde was also assayed in rats that consumed unpolished Thai rice.<br />
Red color strain had the highest antioxidant activities in all tests. It was also showed the highest phenolic content.<br />
Interestingly, total phenolic content was strongly correlated with all anti-oxidant in the methods used: 1,1-diphenyl-2picrylhydrazyl<br />
(r = 0.958, p < 0.01), 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (r = 0.966, p < 0.01) and ferric reducing<br />
antioxidant power (r = 0.992, p < 0.01). Malondialdehyde level in high and low dose treated groups were significantly lower<br />
than that in the control group <strong>of</strong> rats that consumed unpolished Thai rice. Red color <strong>of</strong> unpolished Thai rice, source <strong>of</strong><br />
phenolic compounds, may play a crucial role in oxidative stress prevention. © <strong>2010</strong> Academic Journals.<br />
No.1126<br />
Author (s) : Rattanakul, C.<br />
Title : Effects <strong>of</strong> prolactin and time delay on bone resorption: Mathematical modeling approach<br />
Source : <strong>International</strong> Journal <strong>of</strong> Mathematical Models and Methods in Applied Sciences, 4 (3), pp. 203-211.<br />
Document Type : Article<br />
Keywords : Bone resorption; Estrogen; Parathyroid hormone; Prolactin<br />
<strong>Abstract</strong> : A mathematical model is developed in order to investigate the effect <strong>of</strong> prolactin on the mechanism <strong>of</strong> bone formation and<br />
resorption. By applying the singular perturbation technique to our model, we then obtain the explicit conditions on the<br />
system parameters which ensure the existence <strong>of</strong> limit cycle behavior corresponding to the oscillatory behavior observed in<br />
the clinical data. Numerical simulations are then carried out to support our theoretical analysis. In addition, we extend our<br />
model to investigate the effects <strong>of</strong> estrogen and parathyroid hormone supplements in patients with osteoporosis.<br />
No.1127<br />
Author (s) : Rattanakul, C.<br />
Title : Mathematical modeling <strong>of</strong> bone formation and resorption: Effects <strong>of</strong> parathyroid hormone and prolactin<br />
Source : <strong>International</strong> Conference on Applied Mathematics, Simulation, Modelling - Proceedings, pp. 143-149.<br />
Document Type : Conference Paper<br />
Keywords : Bone resorption; Mathematical model; Parathyroid hormone; Prolactin<br />
<strong>Abstract</strong> : We propose a mathematical model to describe the mechanism <strong>of</strong> bone formation and resorption based on the effects <strong>of</strong><br />
parathyroid hormone and prolactin. The singular perturbation technique is then applied to analyze our model. We derived<br />
the explicit conditions on the system parameters which detect limit cycle behavior which mimics the oscillatory behavior<br />
observed in the clinical data. Finally, numerical investigation is carried out to support our theoretical analysis.<br />
No.1128<br />
Author (s) : Rattanakul, C., Lenbury, Y., Kongson, J., Triampo, W.<br />
Title : The dynamics <strong>of</strong> a nonlinear model <strong>of</strong> signal transduction in human under impulsive depressant drug treatment<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Source : Dynamic Systems and Applications, Volume 19, Issue 3-4, September <strong>2010</strong>, Pages 651-666<br />
Document Type : Article<br />
Keywords : –<br />
<strong>Abstract</strong> : A mathematical model <strong>of</strong> the signal transduction process, involving hormone coupled receptors and an inhibiting enzyme,<br />
under impulsive depressant treatment, is proposed and analyzed. We show that there is a stable periodic solution, at the<br />
vanishing density <strong>of</strong> the ligand bound receptors on the cell membrane and plasmalemma, when the impulsive period is less<br />
than some critical value. The conditions for permanence <strong>of</strong> the system are then given. Finally, it is shown that as the<br />
impulsive period increases beyond a certain critical value, the emergence <strong>of</strong> stable positive periodic solution may be<br />
observed under appropriate conditions on the system parameters. © Dynamic Publishers, Inc.<br />
No.1129<br />
Author (s) : Rattanapan, C., Kantachote, D., Yan, R., Boonsawang, P.<br />
Title : Hydrogen sulfide removal using granular activated carbon bi<strong>of</strong>iltration inoculated with Alcaligenes faecalis T307 isolated from<br />
concentrated latex wastewater<br />
Source : <strong>International</strong> Biodeterioration and Biodegradation, Volume 64, Issue 5, August <strong>2010</strong>, Pages 383-387<br />
Document Type : Article<br />
Keywords : Alcaligenes faecalis; Bi<strong>of</strong>iltration; Granular activated carbon; Hydrogen sulfide<br />
<strong>Abstract</strong> : A novel bi<strong>of</strong>iltration <strong>of</strong> pure culture was developed for H2S removal from synthetic biogas using Alcaligenes faecalis T307<br />
immobilized on granular activated carbon (GAC) as packing materials. The effect <strong>of</strong> operating parameters, including inlet H2S<br />
concentration, H2S flow rate, air flow rate, and height <strong>of</strong> media on the removal efficiency, was evaluated, together with a<br />
product analysis during long-term operation. Besides, the kinetics analysis <strong>of</strong> bi<strong>of</strong>iltration was also studied. The experiment<br />
was performed in three bi<strong>of</strong>ilters. Bi<strong>of</strong>ilters A and B were packed with 20 and 40 cm <strong>of</strong> A. faecalis T307 immobilized GAC,<br />
respectively. Bi<strong>of</strong>ilter C as a control set was packed with 40 cm <strong>of</strong> the GAC without cell immobilization. The result showed<br />
that H2S removal efficiency <strong>of</strong> bi<strong>of</strong>ilters with A. faecalis T307 immobilized GAC was higher than 95% at high inlet<br />
concentrations (200-4000 ppm). Moreover, the complete H2S removal (100%) was found in bi<strong>of</strong>ilters A and B at H2S and air<br />
flow rate <strong>of</strong> 35 and 5.86 l h-1, respectively. Product analysis <strong>of</strong> bi<strong>of</strong>ilters with A. faecalis T307 immobilized GAC was<br />
performed during the long-term operation (60 days). It was found that the major product was elemental sulfur and there was<br />
only a little <strong>of</strong> sulfate ions formed. In addition, the height <strong>of</strong> media did not significantly affect the H2S removal efficiency<br />
when the oxygen was sufficient at air flow rate <strong>of</strong> 5.8 l h-1. Furthermore, in kinetic analysis, the maximum removal rate and<br />
saturation constant <strong>of</strong> H2S were calculated to be Vm = 1.1 kg m-3 s-1 and Ks = 5.2 g m-3, respectively. © <strong>2010</strong> Elsevier Ltd.<br />
No.1130<br />
Author (s) : Rattanaumpawan, P., Lorsutthitham, J., Ungprasert, P., Angkasekwinai, N., Thamlikitkul, V.<br />
Title : Randomized controlled trial <strong>of</strong> nebulized colistimethate sodium as adjunctive therapy <strong>of</strong> ventilator-associated pneumonia<br />
caused by gram-negative bacteria<br />
Source : Journal <strong>of</strong> Antimicrobial Chemotherapy, Volume 65, Issue 12, December <strong>2010</strong>, Article number dkq360, Pages 2645-2649<br />
Document Type : Article<br />
Keywords : Colistin; Healthcare-associated pneumonia; Inhaled therapy<br />
<strong>Abstract</strong> : Background: Cases <strong>of</strong> ventilator-associated pneumonia (VAP) due to multidrug-resistant (MDR) Acinetobacter baumannii and<br />
Pseudomonas aeruginosa are common in hospitalized patients at Siriraj Hospital, Bangkok, Thailand. Parenteral colistimethate<br />
sodium (CMS) has been used for therapy <strong>of</strong> VAP caused by MDR A. baumannii and P. aeruginosa at Siriraj Hospital over the<br />
past few years, with modest favourable outcomes. Objectives: To determine whether nebulized CMS as adjunctive therapy <strong>of</strong><br />
Gram-negative VAP was safe and beneficial. Methods: This was a randomized controlled study in 100 adults who developed<br />
Gram-negative VAP (clinical trial registration number: NCT00920270). All patients received systemic antibiotics according to the<br />
decisions <strong>of</strong> their responsible physicians. The patients were randomized to receive an additional 4 mL <strong>of</strong> nebulized sterile<br />
normal saline (NSS) (n=49) or nebulized CMS equivalent to 75 mg <strong>of</strong> colistin base in 4 mL <strong>of</strong> NSS (n=51) every 12 h until<br />
systemic antibiotic therapy <strong>of</strong> VAP was ended. Results: The baseline characteristics <strong>of</strong> the patients and conventional therapy<br />
<strong>of</strong> VAP in both groups were comparable. Most <strong>of</strong> the cases <strong>of</strong> VAP were caused by MDR A. baumannii and/or P. aeruginosa.<br />
All isolates <strong>of</strong> Gram-negative bacteria were susceptible to colistin. Favourable clinical outcome was 51.0% in the CMS group<br />
and 53.1% in the control group (P=0.84). Patients in the CMS group had significantly more favourable microbiological<br />
outcome when compared with patients in the control group (60.9% versus 38.2%, P=0.03). Bronchospasm was observed in<br />
7.8% <strong>of</strong> patients in the CMS group and in 2.0% <strong>of</strong> patients in the control group (P=0.36). Renal impairment was observed in<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
25.5% <strong>of</strong> patients in the CMS group and in 22.4% <strong>of</strong> patients in the NSS group (P=0.82). Conclusions: Nebulized CMS as<br />
adjunctive therapy <strong>of</strong> Gram-negative VAP seems to be safe. However, a beneficial effect on clinical outcomes <strong>of</strong> adjunctive<br />
nebulized CMS for therapy <strong>of</strong> Gram-negative VAP was not ascertained. © The Author <strong>2010</strong>. Published by Oxford <strong>University</strong><br />
Press on behalf <strong>of</strong> the British Society for Antimicrobial Chemotherapy. All rights reserved.<br />
No.1131<br />
Author (s) : Rattanaumpawan, P.; Sutha, P.; Thamlikitkul, V.<br />
Title : Effectiveness <strong>of</strong> drug use evaluation and antibiotic authorization on patients' clinical outcomes, antibiotic consumption, and<br />
antibiotic expenditures<br />
Source : AMERICAN JOURNAL OF INFECTION CONTROL, Volume: 38, Issue: 1, Pages: 38-43, DOI: 10.1016/j.ajic.2009.04.288, Published:<br />
FEB <strong>2010</strong><br />
Document Type : Article<br />
Keywords : Drug use evaluation; antibiotic authorization; antibiotic consumption; piperacillin/tazobactam; imipenem; meropenem<br />
<strong>Abstract</strong> : Background: Piperacillin/tazobactam, imipenem, and meropenem were inappropriately used in 50% <strong>of</strong> hospitalized patients<br />
at Siriraj Hospital. Siriraj Hospital administrators implemented drug use evaluation ( DUE) and antibiotic authorization for the<br />
aforementioned antibiotics beginning in August 2007. The objective <strong>of</strong> the study was to determine the effectiveness <strong>of</strong><br />
antibiotic authorization on patients' clinical outcomes, antibiotic consumption, and antibiotic expenditures. Methods:<br />
Hospitalized patients who were prescribed piperacillin/tazobactam, imipenem, or meropenem from August to November<br />
2007 were randomly allocated to antibiotic authorization group and no-authorization group. The data on clinical outcomes,<br />
antibiotic consumption, and antibiotic expenditures <strong>of</strong> the patients who received and who did not receive antibiotic<br />
authorization were compared. Results: The patients who received antibiotic authorization (512 prescriptions) had more<br />
favorable clinical outcomes (68.9% vs 60.5%, respectively, P < .01), shorter duration <strong>of</strong> target antibiotics (7.5 days vs 9.3 days,<br />
respectively, P < .01), shorter duration <strong>of</strong> all antibiotics (12.7 days vs 16.4 days, respectively, P < .01), and lower mortality<br />
because <strong>of</strong> infections (29.4% vs 35.4%, respectively, P = .05) than those who did not receive antibiotic authorization (516<br />
prescriptions). The costs <strong>of</strong> target antibiotics and all antibiotics in the authorization group were much less than those in the<br />
no-authorization group. The annual antibiotic cost savings from DUE and antibiotic authorization requirement could be<br />
extrapolated to US $862,704. Conclusion: DUE and antibiotic authorization are effective strategies in reducing antibiotic<br />
consumption and antibiotic expenditures without compromising the patients' clinical outcomes.<br />
No.1132<br />
Author (s) : Rattarasarn, C.; Leelawattana, R.; Soonthornpun, S.<br />
Title : Contribution <strong>of</strong> skeletal muscle mass on sex differences in 2-hour plasma glucose levels after oral glucose load in Thai<br />
subjects with normal glucose tolerance<br />
Source : METABOLISM-CLINICAL AND EXPERIMENTAL, Volume: 59, Issue: 2, Pages: 172-176, DOI: 10.1016/j.metabol.2009.06.029,<br />
Published: FEB <strong>2010</strong><br />
Document Type : Article<br />
Keywords : CARDIOVASCULAR RISK-FACTORS; X-RAY ABSORPTIOMETRY; INSULIN-RESISTANCE; GENDER-DIFFERENCES; WOMEN; SENSITIVITY;<br />
METABOLISM; SECRETION; DISPOSAL; CRITERIA<br />
<strong>Abstract</strong> : Women have higher 2-hour plasma glucose levels after oral glucose challenge than men The smaller skeletal muscle mass in<br />
women may contribute to the higher postload glucose levels The objective <strong>of</strong> this study was to test the hypothesis that the<br />
different amount <strong>of</strong> skeletal muscle mass between men and women contributed to sex difference in postload plasma<br />
glucose levels in subjects with normal glucose tolerance Forty-seven Thai subjects with normal glucose tolerance, 23 women<br />
and 24 age- and body mass index-matched men, were studied Body fat, abdominal fat, and appendages lean mass were<br />
measured by dual-energy x-ray absorptiometry Skeletal muscle insulin sensitivity was determined by euglycemichyperinsulinemic<br />
clamp First-phase insulin secretion and hepatic insulin sensitivity were determined from oral glucose<br />
tolerance data beta-Cell function was estimated from the homeostasis model assessment <strong>of</strong> %B by the homeostasis model<br />
assessment 2 model Correlation and linear regression analysis were performed to identify factors contributing to variances <strong>of</strong><br />
postload 2-hour plasma glucose levels This study showed that women had significantly higher 2-hour plasma glucose levels<br />
and smaller skeletal muscle mass than men. Measures <strong>of</strong> insulin secretion and insulin sensitivity were not different between<br />
men and women. Male sex (r = -0.360, P = 013) and appendages lean mass (r = -0.411, P = 004) were negatively correlated<br />
with 2-hour plasma glucose, whereas log 2-hour insulin (r = 0571, P < 0001), total body fat (r = 0348, P = 016), and log<br />
abdominal fat (r = 0298, P = 042) were positively correlated with 2-hour plasma glucose The correlation <strong>of</strong> 2-hour plasma<br />
412
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
glucose and sex disappeared after adjustment for appendages lean mass By multivariate linear regression analysis, log 2-hour<br />
insulin (beta = 18 9, P < 0001), log 30-minute insulin (beta = -36.3, P = .001), appendages lean mass (beta = -1.0 x 10(-3), P =<br />
018), and hepatic insulin sensitivity index (beta = -17.3, P = -041) explained 54.2% <strong>of</strong> the variance <strong>of</strong> 2-hour plasma glucose In<br />
conclusion, the higher postload 2-hour plasma glucose levels in women was not sex specific but was in part a result <strong>of</strong> the<br />
smaller skeletal muscle mass The early insulin secretion, hepatic insulin sensitivity, and skeletal muscle mass were the<br />
significant factors negatively predicting 2-hour postload plasma glucose levels in Thai subjects with normal glucose tolerance<br />
(C) <strong>2010</strong> Elsevier Inc All rights reserved.<br />
No.1133<br />
Author (s) : Ravinetto, R., Mbonile, L., White, N.<br />
Title : Ethical criteria in clinical research in developing countries: Is there a global standard?<br />
Source : Giornale Italiano di Medicina Tropicale, Volume 15, Issue 1-4, January <strong>2010</strong>, Pages 1-8<br />
Document Type : Review<br />
Keywords : Clinical research; Developing countries; Ethics; Standards<br />
<strong>Abstract</strong> : Clinical researchers in developing countries face multiple challenges related to contextual constraints, poor regulation and<br />
vulnerability <strong>of</strong> trials' subjects. The World Health Organization issued in 1995 its Good Clinical Practices (GCP) Guidelines,<br />
setting globally applicable standards for clinical trials. Non-compliance with GCP principles leaves room for misconduct and<br />
abuse, while a rigid interpretation <strong>of</strong> GCP processes and procedures may unnecessarily increase the research costs and even<br />
prevent research relevant to public health from being carried out. Ethical principles and scientific standards governing<br />
research are universal and should be adopted everywhere, to ensure persons' protection and data's reliability, while avoiding<br />
any North-South ethical divide. However, principles should be translated into simple and effective processes and procedures,<br />
which ensure quality <strong>of</strong> the research and subjects' protection, without putting unnecessary obstacles to public-health<br />
oriented research. It is time to "reinvent" GCP, by updating the 1995 WHO Guidelines in light <strong>of</strong> the 15-year experience <strong>of</strong><br />
worldwide implementation. The revision should include old and new stakeholders (including academic institutions from the<br />
South and the North, NGOs, public-private partnerships, donors, patients associations etc.) and could, by making clearer<br />
distinction between essential and procedural requirements, help researchers and sponsors to design new patient-centered<br />
tools and practices.<br />
No.1134<br />
Author (s) : Rawdaree, P., Deerochanawong, C., Peerapatdit, T., Thongtang, N., Suwanwalaikorn, S., Khemkha, A., Benjasuratwong, Y.,<br />
Boonyavarakul, A., Chetthakul, T., Leelawattana, R., Ngarmukos, C., Viwatwongkasem, C., Pratipanawatr, T., Kosachunhanun, N.<br />
Title : Efficacy and safety <strong>of</strong> generic and original pioglitazone in type 2 diabetes mellitus: A multicenter, a double-blinded,<br />
randomized-controlled study<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 11, November <strong>2010</strong>, Pages 1249-1255<br />
Document Type : Article<br />
Keywords : Diabetes mellitus; Generic; Pioglitazone; Thiazolidinedione; Type 2<br />
<strong>Abstract</strong> : Objective: To compare the efficacy and safety <strong>of</strong> generic (Utmos®) and original (Actos®) 30 mg Pioglitazone tablets. Study<br />
design: A multicenter, parallel randomized, double-blinded, controlled study. Material and Method: Type 2 diabetic patients,<br />
with glycosylated hemoglobin (HbA1c) ≥ 7.0%, who received Metformin not less than 1,000 mg/day over three months were<br />
recruited. Patients were randomized to receive either generic or original Pioglitazone 30 mg/day for 24 weeks. Results: Eightyfive<br />
patients were enrolled, forty-four patients received generic Pioglitazone and forty-one received original Pioglitazone.<br />
There were no significant differences in baseline characteristics between generic and original Pioglitazone group. There were<br />
significantly reduced HbA1c, fasting plasma glucose (FPG) and significantly increased HDL-cholesterol from baseline (p <<br />
0.0001) without statistically differences between the two groups. Headache and edema were found in both groups at<br />
comparable rates (p > 0.05). Conclusion: Generic Pioglitazone (Utmos®) is effective in controlling blood glucose and has<br />
similar effects on lipid pr<strong>of</strong>ile as the original one. Both generic (Utmos®) and original (Actos®) 30 mg Pioglitazone tablets<br />
were not different in the efficacy and safety pr<strong>of</strong>iles.<br />
No.1135<br />
Author (s) : Reiling, L., Richards, J.S., Fowkes, F.J.I., Barry, A.E., Triglia, T., Chokejindachai, W., Michon, P., Tavul, L., Siba, P.M., Cowman, A.F.,<br />
Mueller, I., Beeson, J.G.<br />
Title : Evidence that the erythrocyte invasion ligand PfRh2 is a target <strong>of</strong> protective immunity against Plasmodium falciparum malaria<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Source : Journal <strong>of</strong> Immunology, Volume 185, Issue 10, 15 November <strong>2010</strong>, Pages 6157-6167<br />
Document Type : Article<br />
Keywords : antimalarial agent, genomic DNA, immunoglobulin G, immunoglobulin G1, immunoglobulin G2, immunoglobulin<br />
G3, immunoglobulin G4, ligand, merozoite surface protein 2, monoclonal antibody, Plasmodium falciparum reticulocyte<br />
binding homolog 2a protein, Plasmodium falciparum reticulocyte binding homolog 2b protein, protozoal protein, recombinant<br />
protein, unclassified drug; amino terminal sequence, antigenicity, article, cell invasion, cell mediated cytotoxicity, cluster<br />
analysis, cohort analysis, controlled study, erythrocyte, genetic polymorphism, haplotype, immunity, malaria<br />
falciparum, nonhuman, nucleotide sequence, Papua New Guinea, parasitemia, phagocytosis, Plasmodium falciparum, priority<br />
journal, protection, protein expression, protein function, protein targeting, reinfection, sequence analysis; Adolescent, Amino<br />
Acid Sequence, Antibodies, Protozoan, Antigens, Protozoan, Base Sequence, Child, Cluster Analysis, Enzyme-Linked<br />
Immunosorbent Assay, Genes, Protozoan, Haplotypes, Humans, Malaria, Falciparum, Molecular Sequence Data, Polymerase<br />
Chain Reaction, Polymorphism, Genetic, Protozoan Proteins<br />
<strong>Abstract</strong> : Abs targeting blood-stage Ags <strong>of</strong> Plasmodium falciparum are important in acquired immunity to malaria, but major targets<br />
remain unclear. The P. falciparum reticulocyte-binding homologs (PfRh) are key ligands used by merozoites during invasion <strong>of</strong><br />
erythrocytes. PfRh2a and PfRh2b are functionally important members <strong>of</strong> this family and may be targets <strong>of</strong> protective<br />
immunity, but their potential role in human immunity has not been examined. We expressed eight recombinant proteins<br />
covering the entire PfRh2 common region, as well as PfRh2a-and PfRh2b-specific regions. Abs were measured among a cohort<br />
<strong>of</strong> 206 Papua New Guinean children who were followed prospectively for 6 mo for reinfection and malaria. At baseline, Abs<br />
were associated with increasing age and active infection. High levels <strong>of</strong> IgG to all PfRh2 protein constructs were strongly<br />
associated with protection from symptomatic malaria and high-density parasitemia. The predominant IgG subclasses were IgG1<br />
and IgG3, with little IgG2 and IgG4 detected. To further understand the significance <strong>of</strong> PfRh2 as an immune target, we<br />
analyzed PfRh2 sequences and found that polymorphisms are concentrated in an N-terminal region <strong>of</strong> the protein and seem<br />
to be under diversifying selection, suggesting immune pressure. Cluster analysis arranged the sequences into two main groups,<br />
suggesting that many <strong>of</strong> the haplotypes identified may be antigenically similar. These findings provide evidence suggesting<br />
that PfRh2 is an important target <strong>of</strong> protective immunity in humans and that Abs act by controlling blood-stage parasitemia<br />
and support its potential for vaccine development. Copyright © <strong>2010</strong> by The American Association <strong>of</strong> Immunologists, Inc.<br />
No.1136<br />
Author (s) : Rerks-Ngarm, S., Pitisuttithum, P., Ganguly, N., Zhang, L., Tamashiro, H., Cooper, D.A., Vun, M.C., Bela, B., Ditangco, R., Van Kinh,<br />
N., Bernstein, A., Osmanov, S., Mathieson, B., Kent, S.J., Shao, Y.<br />
Title : Defining the objectives <strong>of</strong> the AIDS vaccine for Asia network: Report <strong>of</strong> the WHO-UNAIDS/Global HIV vaccine enterprise<br />
regional consultation on expanding AIDS vaccine research and development capacity in Asia<br />
Source : Current Opinion in HIV and AIDS, Volume 5, Issue 5, September <strong>2010</strong>, Pages 435-452<br />
Document Type : Review<br />
Keywords : Human immunodeficiency virus vaccine; acquired immune deficiency syndrome, Asia, cellular immunity, clinical trial, health<br />
care planning, health care policy, human, Human immunodeficiency virus prevalence, priority journal, review, vulnerable<br />
population, world health organization; AIDS Vaccines, Asia, Biomedical Research, Clinical Trials as Topic, Drug Discovery, HIV<br />
Infections, Humans, <strong>International</strong> Cooperation<br />
<strong>Abstract</strong> : –<br />
No.1137<br />
Author (s) : Rerks-Ngarm, S.; Premsri, N.; Namwat, C.; Pitisutthithum, P.; Nitayaphan, S.; Kaewkungwal, J.; Kingko, P.; Jirasethasiri, M.; Paris, R.;<br />
Chiu, J.; Kim, J.; Khamboonruang, C.; Thongchareon, P.; Kunasol, P.<br />
Title : Community Engagement: Experience from the Thai Phase III Prime-Boost HIV Vaccine Trial, RV144<br />
Source : AIDS RESEARCH AND HUMAN RETROVIRUSES, Volume: 26, Issue: 10, Pages: A99-A99, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1138<br />
Author (s) : Reutrakul, V., Anantachoke, N., Pohmakotr, M., Jaipetch, T., Yoosook, C., Kasisit, J., Napaswa, C., Panthong, A., Santisuk, T.,<br />
Prabpai, S., Kongsaeree, P., Tuchinda, P.<br />
414
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Anti-HIV1 and anti-inflammatory lupanes from the leaves, twigs, and resin <strong>of</strong> garcinia hanburyi<br />
Source : Planta Medica, Volume 76, Issue 4, <strong>2010</strong>, Pages 368-371<br />
Document Type : Article<br />
Keywords : AntiHIV1; Antiinflammatory; Garcinia hanburyi; Guttiferae; Lupanes<br />
<strong>Abstract</strong> : Two new lupanes, 2-acetoxy-3-hydroxy-19-hydrogen-lup-20(29)-en-28-oic acid (2-acetoxyalphitolic acid) (1) and 2-hydroxy-3acetoxy-19-hydrogen-lup-20(29)-<br />
en-28-oic acid (3-acetoxyalphitolic acid) (2), together with the known betulinic acid (3),<br />
betulin (4), and stimasterol-3-O - D-glucopyranoside (5), were isolated from the leaves and twigs <strong>of</strong> Garcinia hanburyi.<br />
Compounds 13 were also isolated from the resin <strong>of</strong> this plant. The structure <strong>of</strong> 2 was confirmed by single-crystal X-ray<br />
diffraction analysis. All <strong>of</strong> the lupanes (14) displayed anti-HIV1 activities in the anti-HIV1 reverse transcriptase (ICvalues<br />
16.3116.9g/mL) and syncytium assays (EC5.673.6g/mL, SI 1.73.3). Moreover compounds 14 exhibited anti-inflammatory activity<br />
in an ethyl phenylpropiolate (EPP)-induced ear edema model. © Georg Thieme Verlag KG Stuttgart New York.<br />
No.1139<br />
Author (s) : Rey, D.A., Strickland, A.D., Kirui, D., Niamsiri, N., Batt, C.A.<br />
Title : In vitro self-assembly <strong>of</strong> gold nanoparticle-coated poly(3-hydroxybutyrate) granules exhibiting plasmon-induced thermooptical<br />
enhancements<br />
Source : ACS Applied Materials and Interfaces, Volume 2, Issue 7, 28 July <strong>2010</strong>, Pages 1804-1810<br />
Document Type : Article<br />
Keywords : enhanced fluorescence; gold nanoparticles; near-infrared; poly(3-hydroxybutyrate); polyhydroxyalkanoate synthase; selfassembly;<br />
surface enhanced Raman scattering; thermo-optical<br />
<strong>Abstract</strong> : Polyhydroxyalkanoate (PHA) synthase attached to gold nanoparticles (AuNP) produce poly(3-hydroxybutyrate) (PHB) upon the<br />
addition <strong>of</strong> 3-hydroxybutyrate- CoA, and then coalesce to form micrometer-sized AuNP-coated PHB granules. These AuNPcoated<br />
PHB granules are potential theranostic agents that have enhanced imaging capabilities and are capable <strong>of</strong> heating<br />
upon near-infrared laser irradiation. The AuNP-coated PHB exhibited 11-fold enhancement in surface-enhanced Raman<br />
scattering over particles prior polymerization. Stained AuNP-coated PHB exhibited a 6-fold enhancement in fluorescence<br />
intensity as well as a 1.3-fold decrease in photobleaching rate compared to PHB granules alone. The granules were also<br />
shown to emit heat when illuminated at 808 nm with a 3.9-fold increase in heating rate compared to particles alone. © <strong>2010</strong><br />
American Chemical Society.<br />
No.1140<br />
Author (s) : Rhucharoenpornpanich, O., Chamratrithirong, A., Fongkaew, W., Rosati, M.J., Miller, B.A., Cupp, P.K.<br />
Title : Parenting and adolescent problem behaviors: A comparative study <strong>of</strong> sons and daughters in Thailand<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 3, March <strong>2010</strong>, Pages 293-300<br />
Document Type : Article<br />
Keywords : Delinquency; Parenting; Problem behavior; Risk behavior; Thai adolescents<br />
<strong>Abstract</strong> : Objective: Investigates parenting practices and the reported sexual and delinquent behaviors among Thai adolescents, by<br />
focusing on the difference between sons and daughters. Material and Method: Data were derived from 420 families whose<br />
adolescents aged 13-14 were randomly selected from seven districts in Bangkok using the probability proportional to size<br />
(PPS) method. Interviews were conducted with one parent and one adolescent. Results: Female adolescents reported higher<br />
levels <strong>of</strong> parental monitoring, parental rules, communication about sex, and parental disapproval <strong>of</strong> sex, as compared to<br />
males. There were no gender differences in the reported sexual and delinquent behaviors among the adolescents. For males,<br />
high levels <strong>of</strong> parental monitoring, greater perception <strong>of</strong> parent disapproval <strong>of</strong> sex, and being raised by authoritative parents<br />
were associated with less delinquent behaviors. Among females, parental monitoring and parental closeness served as<br />
protective factors against sexual and delinquent behaviors. Conclusion: The findings from the present study point to the<br />
importance <strong>of</strong> promoting family institution and parents' childrearing. These findings should be useful for developing a body <strong>of</strong><br />
knowledge and understanding on adolescent rearing among Thai parents.<br />
No.1141<br />
Author (s) : Ridruechai, C., Mahasirimongkol, S., Phromjai, J., Yanai, H., Nishida, N., Matsushita, I., Ohashi, J., Yamada, N., Moolphate, S.,<br />
Summanapan, S., Chuchottaworn, C., Manosuthi, W., Kantipong, P., Kanitvittaya, S., Sawanpanyalert, P., Keicho, N., Khusmith,<br />
S., Tokunaga, K.<br />
Title : Association analysis <strong>of</strong> susceptibility candidate region on chromosome 5q31 for tuberculosis<br />
415
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Source : Genes and Immunity, Volume 11, Issue 5, July <strong>2010</strong>, Pages 416-422<br />
Document Type : Article<br />
Keywords : chromosome 5q31; family-based association test; Thais; tuberculosis<br />
<strong>Abstract</strong> : Chromosome 5q31 spans the T helper (Th) 2-related cytokine gene cluster, which is potentially important in Th1/Th2<br />
immune responses. The chromosome 5q23.2-31.3 has been recently identified as a region with suggestive evidence <strong>of</strong> linkage<br />
to tuberculosis in the Asian population. With the aim <strong>of</strong> fine-mapping a putative tuberculosis susceptibility locus, we<br />
investigated a family-based association test between the dense single nucleotide polymorphism (SNP) markers within<br />
chromosome 5q31 and tuberculosis in 205 Thai trio families. Of these, 75 SNPs located within candidate genes covering<br />
SLC22A4, SLC22A5, IRF1, IL5, RAD50, IL13, IL4, KIF3A and SEPT8 were genotyped using the DigiTag2 assay. Association analysis<br />
revealed the most significant association with tuberculosis in haplotypes comprising SNPs rs274559, rs274554 and rs274553 <strong>of</strong><br />
SLC22A5 gene (PGlobal 2.02 × 10-6), which remained significant after multiple testing correction. In addition, two haplotypes<br />
within the SLC22A4 and KIF3A region were associated with tuberculosis. Haplotypes <strong>of</strong> SLC22A5 were significantly associated<br />
with the expression levels <strong>of</strong> RAD50 and IL13. The results show that the variants carried by the haplotypes <strong>of</strong> SLC22A4,<br />
SLC22A5 and KIF3A region potentially contribute to tuberculosis susceptibility among the Thai population. © <strong>2010</strong> Macmillan<br />
Publishers Limited All rights reserved.<br />
No.1142<br />
Author (s) : Riedl, J., Wernsdorfer, G., Congpuong, K., Wiedermann, U., Sirichaisinthop, J., Wernsdorfer, W.H.<br />
Title : Synergism between pyronaridine and retinol in Plasmodium vivax in vitro<br />
Source : Wiener Klinische Wochenschrift, Volume 122, Issue SUPPL. 3, October <strong>2010</strong>, Pages 66-70<br />
Document Type : Article<br />
Keywords : Plasmodium vivax; Pyronaridine; Retinol; Synergism<br />
<strong>Abstract</strong> : Estimates <strong>of</strong> the annual number <strong>of</strong> infections with Plasmodium vivax reach 391 million. So far the blood-schizontocidal<br />
therapy with chloroquine remained effective in most parts <strong>of</strong> the world, but reports about emerging resistance are increasing.<br />
The study had the objective <strong>of</strong> determining the pharmacodynamic interaction between pyronaridine and retinol in<br />
Plasmodium vivax, since pyronaridine is a potential alternative for chloroquine and an earlier study had shown strong<br />
synergism between pyronaridine and retinol in Plasmodium falciparum. The study was conducted at the Malaria Clinic <strong>of</strong> Mae<br />
Sot, Tak Province, Thailand, near the border to Myanmar. The in vitro observations followed the method <strong>of</strong> Tasanor.<br />
Successful tests were performed with 44 isolates. The mean IC50, IC90 and IC99 values for pyronaridine were 9.8, 2069.6 and<br />
162446.5 nM. The mean IC50, IC90 and IC99 values for the combinations with retinol (corresponding to the 50th, 65th and<br />
80th percentile <strong>of</strong> the physiological retinol levels in healthy adults) were 1.7, 542.8 and 59379.5 nM for pyronaridine + retinol<br />
"low", for the combination with retinol "medium" they were 0.5, 313.7 and 58891.4 nM and for the combination with retinol<br />
"high" they were 0.2, 96.7 and 16754.3 nM. These results suggest strong synergism between the two substances. © <strong>2010</strong><br />
Springer-Verlag.<br />
No.1143<br />
Author (s) : Rienh<strong>of</strong>f, Hugh Y., Jr.; Viprakasit, Vip; Tay, Lay H.; Harmatz, Paul; Vichinsky, Elliott; Chirnomas, Deborah; Kwiatkowski, Janet;<br />
Tapper, Amy E.; Porter, John B.; Neufeld, Ellis J.<br />
Title : A Phase 1B Dose-Escalation Study to Assess the Safety, Tolerability, Pharmacokinetics and Pharmacodynamics <strong>of</strong> FBS0701, a<br />
Novel Oral Iron Chelator for the Treatment <strong>of</strong> Chronic Iron Overload.<br />
Source : BLOOD, Volume: 116, Issue: 21, Pages: 853-854, Published: NOV 19 <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1144<br />
Author (s) : Riewpaiboon, A., Nuchprayoon, I., Torcharus, K., Indaratna, K., Thavorncharoensap, M., Ubol, B.-O.<br />
Title : Economic burden <strong>of</strong> beta-thalassemia/Hb e and beta-thalassemia major in Thai children<br />
Source : BMC Research Notes, Volume 3, <strong>2010</strong>, Article number 29<br />
Document Type : Article<br />
<strong>Abstract</strong> : Background. Hemoglobin E beta-thalassemia (-thalassemia/Hb E) has a variable severity, and the cost <strong>of</strong> treatment has not<br />
been well studied. The aim <strong>of</strong> this study was to analyze the societal cost <strong>of</strong> caring for children with -thalassemias in Thailand.<br />
416
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
The study was designed as a prevalence-based cost-<strong>of</strong>-illness analysis in a societal perspective. Medical records from three<br />
public hospitals <strong>of</strong> children aged 2-18 years with -thalassemia/Hb E and homozygous -thalassemia were reviewed for direct<br />
medical cost determination. For direct non-medical cost and indirect cost, a family member was interviewed. Findings. It was<br />
found that 201 patients with -thalassemia/Hb E (91%) and homozygous -thalassemia (9%) were recruited for this study.<br />
Ninety-two (46%) were severe thalassemia and 109 (54%) were mild to moderate severity. The annual average cost <strong>of</strong><br />
treatment was US$950; 59% was direct medical cost, 17% direct non-medical cost, and 24% indirect cost. The costs were<br />
differentiated by some potential predictors. Significant predictor variables were: Hospital, health insurance scheme, blood<br />
transfusion pattern, and iron chelation drug use. Conclusions. The average annual cost per patient was calculated, and the<br />
cost model was estimated. These would be applied for national planning, economic evaluation <strong>of</strong> treatment and prevention<br />
interventions, and budget impact analysis. © <strong>2010</strong> Riewpaiboon et al; licensee BioMed Central Ltd.<br />
No.1145<br />
Author (s) : Riewpaiboon, A.; Koopitakkajorn, T.; Kumluang, S.; Chaikledkaew, U.; Khiaocharoen, O.<br />
Title : DEVELOPMENT OF STANDARD COST LIST FOR ECONOMIC EVALUATION IN THAILAND<br />
Source : VALUE IN HEALTH, Volume: 13, Issue: 7, Pages: A506-A506, Published: NOV <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1146<br />
Author (s) : Rirattanapong, P., Smutkeeree, A., Surarit, R., Saendsirinavin, C., Kunanantsak, V.<br />
Title : Effects <strong>of</strong> fluoride dentifrice on remineralization <strong>of</strong> demineralized primary enamel<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 1, January <strong>2010</strong>, Pages 243-249<br />
Document Type : Article<br />
Keywords : Demineralization; Dentrifice; Fluoride; Remineralization<br />
<strong>Abstract</strong> : This study was performed to compare the remineralizing effects <strong>of</strong> various concentrations <strong>of</strong> fluoride containing dentifrices<br />
against artificial demineralization <strong>of</strong> primary enamel. One hundred twenty primary incisors were partly covered with a nail<br />
varnish, leaving a lxl mm window, then placed in demineralizing solution for 96 hours to produce artificial carious lesions 60-<br />
100 μm in depth. They were assigned to 8 groups (A to H; n=15). Groups A-D were exposed to a half pea-sized portion <strong>of</strong><br />
dentifrice (0.16 g) and groups E-H were exposed to a pea-sized portion <strong>of</strong> dentifrice (0.32 g), both groups with fluoride<br />
concentrations <strong>of</strong> 0, 250, 500 and 1,000 ppm. The pH-cycling method was carried out for 7 days, then the teeth were cut<br />
through the lesions and examined under a polarized light microscope; photographs were taken and analyzed. Lesion depth<br />
was measured using a computerized method using the Image-Pro ® Plus Program. The results were analyzed using the One<br />
way ANOVA and LSD tests. The mean lesion depth in the 2 non-fluoridated control groups (A and E) were significantly deeper<br />
than in the fluoridated groups. There were no differences found between the half peasized and pea-sized dentifrice.<br />
No.1147<br />
Author (s) : Ritprajak, P., Hashiguchi, M., Tsushima, F., Chalermsarp, N., Azuma, M.<br />
Title : Keratinocyte-associated B7-H1 directly regulates cutaneous effector CD8+ T cell responses<br />
Source : Journal <strong>of</strong> Immunology, Volume 184, Issue 9, 1 May <strong>2010</strong>, Pages 4918-4925<br />
Document Type : Article<br />
Keywords : cytokeratin 14, gamma interferon, hapten, programmed death 1 ligand 1, apoptosis regulatory protein, autacoid, B7<br />
antigen, leukocyte antigen, membrane protein, PDCD1 protein, human, Pdcd1lg1 protein, mouse, peptide; animal cell, animal<br />
experiment, animal model, apoptosis, article, CD4+ T lymphocyte, CD8+ T lymphocyte, cell migration, cell proliferation, cell<br />
stimulation, contact allergy, controlled study, cytokine production, dendritic cell, down regulation, gene<br />
overexpression, immune response, immunoregulation, keratinocyte, lymph node, lymphocyte<br />
function, mouse, nonhuman, priority journal, promoter region, protein expression, skin sensitization, transgenic<br />
mouse, animal, Bagg albino mouse, biosynthesis, C57BL mouse, cell culture, contact<br />
dermatitis, genetics, human, immunology, leukemia cell line, metabolism, pathology, physiology, skin; Animals, Antigens,<br />
CD, Antigens, CD80, Apoptosis Regulatory Proteins, CD8-Positive T-Lymphocytes, Cells, Cultured, Dermatitis, Contact, Down-<br />
Regulation, Humans, Inflammation Mediators, Jurkat Cells, Keratin-14, Keratinocytes, Membrane Glycoproteins, Mice, Mice,<br />
Inbred BALB C, Mice, Inbred C57BL, Mice, Transgenic, Peptides, Promoter Regions, Genetic, Skin<br />
417
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : Keratinocytes (KCs) may play important roles for maintenance <strong>of</strong> peripheral tolerance in the upper layers <strong>of</strong> the skin.<br />
Coinhibitory signals mediated via the programmed death (PD)-1 and its ligand B7-H1 (PD-L1/CD274) are crucial for the<br />
downregulation <strong>of</strong> T cell immune responses and for the maintenance <strong>of</strong> peripheral tolerance. In this study, to investigate the<br />
role <strong>of</strong> KC-expressed B7-H1 in the regulation <strong>of</strong> T cell immune responses, we generated transgenic (tg) mice overexpressing<br />
B7-H1 under the control <strong>of</strong> keratin 14 (K14) promoter (K14-B7-H1 tg). K14-B7-H1 tg mice displayed impaired contact<br />
hypersensitivity (CH) responses to primary and secondary hapten challenges. The K14-B7-H1 tg mice did not exhibit<br />
substantial impairment <strong>of</strong> cutaneous dendritic cell migration after sensitization and <strong>of</strong> hapten-specific proliferation and IFN-γ<br />
production <strong>of</strong> CD4+ and CD8+ T cells in the draining lymph nodes, suggesting that overexpression <strong>of</strong> B7-H1 on KCs did not<br />
affect the induction phase <strong>of</strong> the CH response. The systemic or s.c. injection <strong>of</strong> hapten-sensitized T cells into the K14-B7-H1<br />
tg mice did not efficiently induce the CH response. IFN-γ expression and apoptosis <strong>of</strong> KCs in the challenged ears were<br />
impaired in K14-B7-H1 tg mice. IFN-γ production by presensitized CD8+ T cells stimulated with hapten-pulsed KCs was<br />
markedly impaired for the KCs obtained from the K14-B7-H1 tg mice but was restored by the addition <strong>of</strong> an anti-B7-H1 mAb.<br />
These results suggest that KC-associated B7-H1 directly downregulates the effector function <strong>of</strong> CD8+ T cells by associating<br />
with PD-1 at local inflammatory sites and that it plays a role in peripheral T cell tolerance against exogenous Ags. Copyright ©<br />
<strong>2010</strong> by The American Association <strong>of</strong> Immunologists, Inc.<br />
No.1148<br />
Author (s) : Riyajan, S.-A., Sakdapipanich, J.T.<br />
Title : Characterization <strong>of</strong> biodegradable semi-interpenetrating polymer based on poly(vinyl alcohol) and sodium alginate containing<br />
natural neem (Azadirachta indica) for controlled release application<br />
Source : Polymer <strong>International</strong>, Volume 59, Issue 8, August <strong>2010</strong>, Pages 1130-1140<br />
Document Type : Article<br />
Keywords : Biodegradable polymer; Encapsulation; IPN; Neem; PVA; Sodium alginate<br />
<strong>Abstract</strong> : A biodegradable novel semi-interpenetrating polymer network based on poly(vinyl alcohol) (PVA) and sodium alginate<br />
containing neem (Azadirachta indica) in the presence <strong>of</strong> azadirachtin-A (neem Aza-A) as well as glutaraldehyde as a<br />
crosslinking agent was prepared for use in the controlled released <strong>of</strong> neem Aza-A. This is necessary because neem Aza-A is<br />
not stable in the environment. The neem Aza-A-containing beads were prepared using various experimental parameters, such<br />
as the extent <strong>of</strong> crosslinking and the amount <strong>of</strong> loading, in order to optimize the process variables. The chemical structure <strong>of</strong><br />
the capsule wall was evaluated through X-ray diffraction. In addition, the swelling behaviour <strong>of</strong> the capsules and their<br />
thermal stability were investigated. The strength <strong>of</strong> the capsule wall depended on the PVA in the matrix and the crosslinking<br />
density. Scanning electron microscopy, electron probe microanalysis and atomic force microscopy data indicated that the<br />
structure <strong>of</strong> the bead walls is rough and nonporous. Swelling results indicated that swelling <strong>of</strong> the polymeric beads decreases<br />
with increasing exposure time to the crosslinking agent. At particular intervals, the remaining concentration <strong>of</strong> neem Aza-A was<br />
analysed using high-performance liquid chromatography. The release data were fitted to an empirical equation to estimate<br />
the kinetic parameters. The degree <strong>of</strong> release <strong>of</strong> neem Aza-A was controlled by the parameter conditions. © <strong>2010</strong> Society <strong>of</strong><br />
Chemical Industry.<br />
No.1149<br />
Author (s) : Riyong, D., Waikagul, J., Panasoponkul, C., Choochote, W., Ito, A., Dekumyoy, P.<br />
Title : Size and charge antigens <strong>of</strong> Dir<strong>of</strong>ilaria immitis adult worm for IgG-ELISA diagnosis <strong>of</strong> bancr<strong>of</strong>tian filariasis<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 2, March <strong>2010</strong>, Pages 285-297<br />
Document Type : Article<br />
Keywords : Column chromatography; Dir<strong>of</strong>ilaria antigen; IgG-ELISA; Iso-electric focusing; Og4C3-ELISA<br />
<strong>Abstract</strong> : We used Dir<strong>of</strong>ilaria immitis adult worm antigens to develop an IgG-ELISA, then used this to evaluate 30 serum samples <strong>of</strong><br />
patients with proven Wuchereria bancr<strong>of</strong>ti infection, 131 samples <strong>of</strong> patients with other parasitic diseases and 30 serum<br />
samples <strong>of</strong> healthy controls. The D. immitis antigen was prepared using two methods: Sephacryl S-200 chromatography and<br />
iso-electric focusing with a Rot<strong>of</strong>or cell. This was done to determine the best method for diagnosing W. bancr<strong>of</strong>ti filariasis.<br />
Before fractionation, crude male D. immitis antigen yielded 100% sensitivity and 60.8% specificity, and crude female antigen<br />
yielded 80% sensitivity and 52.8% specificity, respectively, to detect W. bancr<strong>of</strong>ti infection. After gel filtration chromatography,<br />
the male D. immitis antigen, called MP1, yielded 100% sensitivity and 95% specificity, and female D. immitis antigen, called<br />
FmP1, gave 100% sensitivity and 59.6% specificity, to detect W. bancr<strong>of</strong>ti infection. Using iso-electricfocusing, both male and<br />
female crude D. immitis antigens (Iso-MF and Iso-FmF, respectively) were separated mechanically into 20 iso-fractions (F1-F20)<br />
418
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
each. By preliminary screening with ELISA, using pooled positive and negative sera, Iso-MF10, pH 7.5, and Iso-FmF14, pH 7.6,<br />
were selected. Iso-MF10 gave 100% sensitivity and 96.9% specificity, and Iso-FmF14 gave 100% sensitivity and 64% specificity.<br />
In the study, Og4C3-ELISA, for the detection <strong>of</strong> circulating filarial antigen, was also used to analyze these serum samples, it<br />
gave 87.6% sensitivity and 99.4% specificity to detect W. bancr<strong>of</strong>ti infection. Male D. immitis antigens, MP1 and Iso-MF10, gave<br />
high sensitivity and specificity, and appear to be the best choices for use in an ELISA to diagnose bancr<strong>of</strong>tian filariasis.<br />
No.1150<br />
Author (s) : Robinson, M.J., Erlwein, O.W., Kaye, S., Weber, J., Cingoz, O., Patel, A., Walker, M.M., Kim, W.-J., Uiprasertkul, M., C<strong>of</strong>fin, J.M.,<br />
McClure, M.O.<br />
Title : Mouse DNA contamination in human tissue tested for XMRV<br />
Source : Retrovirology, Volume 7, 20 December <strong>2010</strong>, Article number 108<br />
Document Type : Article<br />
Keywords : mitochondrial DNA; article, controlled study, DNA contamination, DNA extraction, gene sequence, human, human tissue, long<br />
terminal repeat, major clinical study, male, mouse, nonhuman, nucleotide sequence, polymerase chain<br />
reaction, prevalence, prostate cancer, United States, Xenotropic MuLV related virus, animal, genetics, isolation and<br />
purification, molecular genetics, oncogene, prostate, prostate tumor, virology; Gammaretrovirus, Intracisternal Aparticles,<br />
Murinae, Xenotropic murine leukemia virus; Animals, Base Sequence, DNA Contamination, DNA,<br />
Mitochondrial, Genes, Intracisternal A-Particle, Humans, Male, Mice, Molecular Sequence Data, Polymerase Chain<br />
Reaction, Prostate, Prostatic Neoplasms, Xenotropic murine leukemia virus-related virus<br />
<strong>Abstract</strong> : Background: We used a PCR-based approach to study the prevalence <strong>of</strong> genetic sequences related to a gammaretrovirus,<br />
xenotropic murine leukemia virus-related virus, XMRV, in human prostate cancer. This virus has been identified in the US in<br />
prostate cancer patients and in those with chronic fatigue syndrome. However, with the exception <strong>of</strong> two patients in<br />
Germany, XMRV has not been identified in prostate cancer tissue in Europe. Most putative associations <strong>of</strong> new or old human<br />
retroviruses with diseases have turned out to be due to contamination. We have looked for XMRV sequences in DNA<br />
extracted from formalin-fixed paraffin- embedded prostate tissues. To control for contamination, PCR assays to detect either<br />
mouse mitochondrial DNA (mtDNA) or intracisternal A particle (IAP) long terminal repeat DNA were run on all samples, owing<br />
to their very high copy number in mouse cells.Results: In general agreement with the US prevalence, XMRV-like sequences<br />
were found in 4.8% <strong>of</strong> prostate cancers. However, these were also positive, as were 21.5% <strong>of</strong> XMRV-negative cases, for IAP<br />
sequences, and many, but not all were positive for mtDNA sequences.Conclusions: These results show that contamination<br />
with mouse DNA is widespread and detectable by the highly sensitive IAP assay, but not always with less sensitive assays,<br />
such as murine mtDNA PCR. This study highlights the ubiquitous presence <strong>of</strong> mouse DNA in laboratory specimens and <strong>of</strong>fers a<br />
means <strong>of</strong> rigorous validation for future studies <strong>of</strong> murine retroviruses in human disease. © <strong>2010</strong> Robinson et al; licensee<br />
BioMed Central Ltd.<br />
No.1151<br />
Author (s) : Roekring, S., Smith, D.R.<br />
Title : Induction <strong>of</strong> apoptosis in densovirus infected Aedes aegypti mosquitoes<br />
Source : Journal <strong>of</strong> Invertebrate Pathology, Volume 104, Issue 3, July <strong>2010</strong>, Pages 239-241<br />
Document Type : Article<br />
Keywords : Aedes; Apoptosis; Brevidensovirus; Caspase; Densovirus<br />
<strong>Abstract</strong> : The mechanism <strong>of</strong> death in densovirus infected mosquitoes remains unexplored. This study investigated the cellular<br />
consequences <strong>of</strong> densovirus infection in Aedes aegypti mosquitoes after a second generation challenge with a densovirus<br />
isolated from adult Aedes albopictus mosquitoes in Thailand (AThDNV). Specimens were analyzed by TUNEL assay,<br />
fluorescent in situ hybridization (FISH) and a calorimic assay to detect activation <strong>of</strong> caspase 3-like activity. After challenge,<br />
moribund mosquitoes showed considerable evidence <strong>of</strong> TUNEL positive cells. The caspase 3-like activity assay showed that<br />
the presence <strong>of</strong> TUNEL positive cells was associated with increased levels <strong>of</strong> activated caspase 3-like activity in AThDNV<br />
infected mosquitoes. © <strong>2010</strong> Elsevier Inc.<br />
No.1152<br />
Author (s) : Rojanarata, T., Opanasopit, P., Ngawhirunpat, T., Saehuan, C., Wiyakrutta, S., Meevootisom, V.<br />
Title : A simple, sensitive and green bienzymatic UV-spectrophotometric assay <strong>of</strong> amoxicillin formulations<br />
Source : Enzyme and Microbial Technology, Volume 46, Issue 3-4, 5 March <strong>2010</strong>, Pages 292-296<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Document Type : Article<br />
Keywords : Amoxicillin; Enzymatic method; Spectrophotometry<br />
<strong>Abstract</strong> : A simple, fast, sensitive and inexpensive UV-spectrophotometric method for the determination <strong>of</strong> amoxicillin in<br />
pharmaceutical preparations has been developed based on two enzymatic reactions. In this method, d-4hydroxyphenylglycine<br />
side chain <strong>of</strong> amoxicillin was selectively cleaved <strong>of</strong>f by penicillin acylase. Subsequently, it was reacted<br />
with 2-oxoglutarate, by the catalysis <strong>of</strong> d-phenylglycine aminotransferase, to yield the product with high UV absorption<br />
namely 4-hydroxybenzoylformate. The amount <strong>of</strong> amoxicillin was then determined as a change in absorbance at 335 nm. In<br />
this work, the assay conditions were studied and optimized and the method was validated. The calibration curve presented<br />
an excellent linearity with r2 <strong>of</strong> 0.9998 (0-100 μM amoxicillin). Detection and quantitation limits were 0.77 and 2.55 μM,<br />
respectively. Good accuracy and precision were obtained when the method was tested with amoxicillin capsules and powder<br />
for oral suspension. No interference from common excipients in the formulations or degradation products was observed.<br />
Finally, since all procedures were performed without the use <strong>of</strong> any organic solvents or hazardous chemicals which were<br />
detrimental to the environment and had a low consumption <strong>of</strong> reagents, this proposed assay was an ideal green analytical<br />
method suitable for the quality control <strong>of</strong> amoxicillin in pharmaceuticals. © 2009 Elsevier Inc. All rights reserved.<br />
No.1153<br />
Author (s) : Rojanarata, T.; Opanasopit, P.; Ngawhirunpat, T.; Saehuan, C.; Wiyakrutta, S.; Meevootisom, V.<br />
Title : Amoxicillin Assay Based on Penicillin Acylase and D-Phenylglycine Aminotransferase<br />
Source : JOURNAL OF BIOTECHNOLOGY, Volume: 150, Supplement: 1, Pages: S427-S427, DOI: 10.1016/j.jbiotec.<strong>2010</strong>.09.596, Published:<br />
NOV <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1154<br />
Author (s) : Rojchanaprasart, N., Chai-aroon, T., Phlainoi, N., Juntarashote, K.<br />
Title : Competency <strong>of</strong> small-scale fishers in fishery co-management<br />
Source : Kasetsart Journal - Social Sciences, Volume 31, Issue 3, September <strong>2010</strong>, Pages 424-435<br />
Document Type : Article<br />
Keywords : Competency; Fishery co-management; Small-scale fishers<br />
<strong>Abstract</strong> : This study aims to demonstrate the competency level <strong>of</strong> small-scale fishers for operating fishery comanagement. The study<br />
was conducted in three phases. The first phase deals with constructing and verifying competency model by reviewing<br />
literature to construct the initial model and in-depth interviewing with the fishery co-management experts. Phase II is<br />
competency assessment using questionnaires to interview smallscale fishers. A total <strong>of</strong> 392 fishers which comprised <strong>of</strong> 204<br />
and 188 persons from the upper and lower provinces on the Andaman coastline, respectively, were sampled. Finally, Phase III<br />
concerns with verifying the assessed results by expert focus-groups. Examination <strong>of</strong> each competency aspect revealed that,<br />
from the nine competencies, small-scale fishers in the upper province had knowledge <strong>of</strong> geo-ecological and local resource at<br />
a leading level which was higher than small-scale fishers in the lower province whose knowledge was at the application level.<br />
However, smallscale fishers in these provinces had the learning person competency at the beginner level. Considering all<br />
aspects <strong>of</strong> competency simultaneously by cluster analysis, it was found small-scale fishers could be divided into three<br />
groups: those who had knowledge <strong>of</strong> fishery law and geo-ecology but weak in experience; those who were learning person<br />
and had holistic thinking; and those who lacked volunteer spirit, leadership, and communication. The study suggests that the<br />
competency <strong>of</strong> small-scale fishers in fishery co-management which should be developed primarily is learning person, follow<br />
by holistic thinking, volunteer spirit, leadership, and communication competencies.<br />
No.1155<br />
Author (s) : Rojruthai, P., Sakdapipanich, J.T., Takahashi, S., Hyegin, L., Noike, M., Koyama, T., Tanaka, Y.<br />
Title : In vitro synthesis <strong>of</strong> high molecular weight rubber by Hevea small rubber particles<br />
Source : Journal <strong>of</strong> Bioscience and Bioengineering, Volume 109, Issue 2, February <strong>2010</strong>, Pages 107-114<br />
Document Type : Article<br />
Keywords : Biosynthesis; Hevea brasiliensis; Latex; Natural rubber; Rubber transferases; Small rubber particles<br />
<strong>Abstract</strong> : Hevea brasiliensis is one <strong>of</strong> few higher plants producing the commercial natural rubber used in many significant applications.<br />
The biosynthesis <strong>of</strong> high molecular weight rubber molecules by the higher plants has not been clarified yet. Here, the in vitro<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
rubber biosynthesis was performed by using enzymatically active small rubber particles (SRP) from Hevea. The mechanism <strong>of</strong><br />
the in vitro rubber synthesis was investigated by the molecular weight distribution (MWD). The highly purified SRP prepared by<br />
gel filtration and centrifugation in the presence <strong>of</strong> Triton® X-100 showed the low isopentenyl diphosphate (IPP) incorporation<br />
for the chain extension mechanism <strong>of</strong> pre-existing rubber. The MWD <strong>of</strong> in vitro rubber elongated from the pre-existing rubber<br />
chains in SRP was analyzed for the first time in the case <strong>of</strong> H. brasiliensis by incubating without the addition <strong>of</strong> any initiator.<br />
The rubber transferase activity <strong>of</strong> 70% incorporation <strong>of</strong> the added IPP (w/w) was obtained when farnesyl diphosphate was<br />
present as the allylic diphosphate initiator. The in vitro synthesized rubber showed a typical bimodal MWD <strong>of</strong> high and low<br />
molecular weight fractions in GPC analysis, which was similar to that <strong>of</strong> the in vivo rubber with peaks at around 106 and<br />
105 Da or lower. The reaction time independence and dependence <strong>of</strong> molecular weight <strong>of</strong> high and low molecular weight<br />
fractions, respectively, indicated that the high molecular weight rubber was synthesized from the chain extension <strong>of</strong> preexisting<br />
rubber molecules whereas the lower one was from the chain elongation <strong>of</strong> rubber molecules newly synthesized from<br />
the added allylic substrates. © 2009 The Society for Biotechnology, Japan.<br />
No.1156<br />
Author (s) : Rojsanga, P., Sukhthankar, M., Krisanapun, C., Gritsanapan, W., Lawson, D.B., Baek, S.J.<br />
Title : In vitro anti-proliferative activity <strong>of</strong> alcoholic stem extract <strong>of</strong> coscinium fenestratum in human colorectal cancer cells<br />
Source : Experimental and Therapeutic Medicine, Volume 1, Issue 1, January <strong>2010</strong>, Pages 181-186<br />
Document Type : Article<br />
Keywords : Activating transcription factor 3; Coscinium fenestratum; Cyclin D1; Non-steroidal anti-inflammatory drug-activated gene-<br />
1; Peroxisome proliferator-activated receptor γ<br />
<strong>Abstract</strong> : Coscinium fenestratum (Gaertn.) Colebr. is traditionally used for the treatment <strong>of</strong> cancer, arthritis and diabetes mellitus. the<br />
purpose <strong>of</strong> this study was to determine the molecular mechanisms by which this plant shows benefcial effects. an 80%<br />
ethanolic extract <strong>of</strong> C. fenestratum (80et) was separated by its polarity into dichloromethane (DCM) and aqueous fractions<br />
(WF), and the anti-proliferative effects <strong>of</strong> 80et, dcm and WF were investigated. Berberine, one <strong>of</strong> the major components <strong>of</strong> C.<br />
fenestratum, was used as a control. the 80ET, DCM, WF and berberine showed anti-proliferative activity as assessed by cell<br />
growth assay. Subsequently, the pro-apoptotic proteins NAG-1 and ATF3 were increased and the cell cycle protein cyclin D1<br />
was decreased by the extract and its fractions. interestingly, only the DCM fraction exhibited the induction <strong>of</strong> peroxisome<br />
proliferator-activated receptor γ (PPARγ) binding activity, which represents a pro-apoptotic activity in colorectal cancer cells.<br />
the overall results <strong>of</strong> this study indicate that the extract from this plant has anti-proliferative activity through the activation <strong>of</strong><br />
pro-apoptotic proteins and PPARγ, and may have potential as a preventive regimen in the treatment <strong>of</strong> cancer.<br />
No.1157<br />
Author (s) : Romphruk, A.V., Romphruk, A., Kongmaroeng, C., Klumkrathok, K., Paupairoj, C., Leelayuwat, C.<br />
Title : HLA class i and II alleles and haplotypes in ethnic Northeast Thais<br />
Source : Tissue Antigens, Volume 75, Issue 6, June <strong>2010</strong>, Pages 701-711<br />
Document Type : Article<br />
Keywords : Ethnic Northeast Thais; Haplotypes; Human leukocyte antigen; Major histocompatibility complex<br />
<strong>Abstract</strong> : Allele frequencies (AFs) and haplotypic associations <strong>of</strong> human leukocyte antigen (HLA) class I and II were investigated in 400<br />
unrelated, healthy, ethnic Northeast Thais. HLA-A, -B, -Cw, -DRB1 and -DQB1 were typed by polymerase chain reactionsequence<br />
specific primer, -sequence specific oligonucleotide probe and -single-strand conformation polymorphism methods.<br />
In this population, 17 HLA-A, 26 HLA-B, 15 HLA-Cw, 26 HLA-DRB1 and 13 HLA-DQB1 alleles (or groups <strong>of</strong> alleles) were found.<br />
AFs > 10% included A*11 (23.3%), 24 (18.8%), 0207 (14.4%), 33 (11.5%), 0203 (10.6%); B*4601 (13.9%); Cw*07(01-03) (18.5%),<br />
01 (15.9%), 04 (12.0%), 0304 (10.6%); DRB1*1502 (18.5%), 1202 (13.4%); DQB1*0502 (20.3%), 0501 (16.3%), 0301 (14.1%) and<br />
02 (10.9%). The most common <strong>of</strong> 2-locus haplotypes included A*0207-B*4601 (9.3%), B*4601-Cw*01 (13.5%), B*5801-<br />
DRB1*0301 (5.8%) and DRB1*1502-DQB1*0501 (14.1%). Of the 49 five-locus HLA haplotypes identified, 24 were confirmed in<br />
31 family studies: the most common being; A*33-Cw*0302-B*5801-DRB1*0301-DQB1*02 (4.6%), A*0207-Cw*01-B*4601-<br />
DRB1*09-DQB1*0303 (3.4%) and A*33-Cw*07(01-03)-B*44-DRB1*07-DQB1*02 (2.6%). Apparently, the HLA-B*46-carrying<br />
haplotype is fragmented in ethnic Northeast Thais, including seven haplotypes with different HLA-A and HLA-DR/DQ<br />
combinations. One <strong>of</strong> these haplotypes (A*11-Cw*01-B*4601- DRB1*1202-DQB1*0502) has not been reported in other Asians.<br />
The results indicated that there were marked differences in the distribution <strong>of</strong> HLA alleles and haplotypes between ethnic<br />
Northeast Thais and other ethnic groups in Southeast and East Asia. These results also dictate that future studies <strong>of</strong> HLA<br />
alleles and diseases need precise identification <strong>of</strong> ethnically and geographically matched controls. The HLA allele and<br />
421
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
haplotype analyses in this large sample provide baseline information on ethnic Northeast Thais for anthropological studies<br />
and for determining HLA allele/haplotype frequencies when searching for HLA-compatible donors for unrelated bone marrow<br />
transplantation. © <strong>2010</strong> John Wiley & Sons A/S.<br />
No.1158<br />
Author (s) : Ronpirin, C.; Achariyakul, M.; Tencomnao, T.; Wongpiyabovorn, J.; Chaicumpa, W.<br />
Title : Up-regulation <strong>of</strong> Id1 in peripheral blood <strong>of</strong> psoriatic patients<br />
Source : GENETICS AND MOLECULAR RESEARCH, Volume: 9, Issue: 4, Pages: 2239-2247, DOI: 10.4238/vol9-4gmr963, Published: <strong>2010</strong><br />
Document Type : Article<br />
Keywords : PBMCs; Psoriasis; Gene expression analysis; Proliferation; Id1; mRNA<br />
<strong>Abstract</strong> : Although the precise causes <strong>of</strong> psoriasis are unclear, it is widely accepted that psoriasis is a disorder in which factors in the<br />
immune system, enzymes, and other biochemical substances that regulate skin cell division are impaired, leading to rapid<br />
proliferation <strong>of</strong> keratinocytes and incomplete keratinization. Expression <strong>of</strong> the helix-loop-helix transcription factor Id1<br />
(inhibitor <strong>of</strong> differentiation/DNA binding), functioning as an inhibitor <strong>of</strong> differentiation, is known to increase in psoriatic skin.<br />
However, the molecular involvement <strong>of</strong> this particular biomarker in the psoriatic immune system remains to be elucidated.<br />
We measured Id1 mRNA expression in peripheral blood mononuclear cells (PBMCs) <strong>of</strong> psoriatic patients and healthy controls<br />
using semi-quantitative reverse transcriptase-PCR. The normalized level <strong>of</strong> Id1 transcripts in psoriatic patients was about 2-fold<br />
higher than that in controls (P < 0.05). When we examined the proliferation rate <strong>of</strong> PBMCs, the stimulation index obtained<br />
from the phytohemagglutinin stimulation assay was not significantly different in psoriatic patients. In patients with psoriasis,<br />
there was no correlation between the stimulation index and the psoriasis area severity index. We suggest that Id1 has a role<br />
in causing psoriatic immune cell symptoms.<br />
No.1159<br />
Author (s) : Roongpisuthipong, A., Chalermchockcharoenkit, A., Sirimai, K., Wanitpongpan, P., Jaishuen, A., Foongladda, S., Kongkergkiat, N.,<br />
Prymanee, J.<br />
Title : Safety and efficacy <strong>of</strong> a new imidazole fungicide, Sertaconazole, in the treatment <strong>of</strong> fungal vulvo-vaginitis: A comparative<br />
study using Fluconazole and Clotrimazole<br />
Source : Asian Biomedicine, Volume 4, Issue 3, June <strong>2010</strong>, Pages 443-448<br />
Document Type : Article<br />
Keywords : Clotrimazole; Efficacy; Fluconazole; Fungal vulvo-vaginitis; Recurrence; Risk factors; Sertaconazole<br />
<strong>Abstract</strong> : Background: Sertaconazole is a new imidazole fungicide introduced for vulvo-vaginal candidiasis. It has an azole group with<br />
benzothiophene that inhibits biosynthesis <strong>of</strong> ergosterol and brings about a massive leak <strong>of</strong> cytoplasm with consequent fungal<br />
cell death. Objective: Assess the safety and efficacy <strong>of</strong> Sertaconazole in the treatment <strong>of</strong> fungal vulvo-vaginitis for comparison<br />
with Fluconazole and Clotrimazole. Subjects and methods: One-hundred eighty-eight outpatients with fungal vulvo-vaginitis<br />
were recruited at Siriraj Hospital, Thailand between August 31, 2004 and January 30, 2006. The patients were given<br />
Sertaconazole, Fluconazole, or Clotrimazole, and received vaginal swab and culture for fungus at seventh and 28th days after<br />
treatment. Results: Out <strong>of</strong> 188 cases, 177 cases were followed-up completely. Sertaconazole group included 66 cases where<br />
35 cure, 20 fail, and 7 recurrent cases. Fluconazole group included 60 cases and had 37 cure, six fail, and 20 recurrence cases.<br />
Clotrimazole group included 55 cases and had 32 cure, nine fail and 11 recurrent cases. There were risk factors <strong>of</strong> fungal<br />
vulvo-vaginitis, including frequent micturition and small toilet shower flushing. Conclusion: Sertaconazole had similar<br />
effectiveness and less side-effect as compared with Fluconazole and Clotrimazole. It appeared to work well with lowest<br />
recurrence.<br />
No.1160<br />
Author (s) : Roongruangchai, K., Kummalue, T., Sookkua, T., Roongruangchai, J.<br />
Title : Comparison <strong>of</strong> pouzolzia indica methanolic extract and virkon® against cysts <strong>of</strong> acanthamoeba SPP<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 4, July <strong>2010</strong>, Pages 776-784<br />
Document Type : Article<br />
Keywords : Acanthamoeba spp; Pouzolzia indica benn; Sem; Tem; Virkon®<br />
<strong>Abstract</strong> : The present study was conducted to investigate the morphological and structural changes <strong>of</strong> Acanthamoeba cysts after being<br />
treated with various concentrations <strong>of</strong> Pouzolzia indica methanolic extract fraction 3 (methanol eluted) and Virkon® solution.<br />
Changes in the Acanthamoeba cysts were detected by light microscopy, scanning electron microscopy and transmission<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
electron microscopy. The results show Acanthamoeba cysts were killed by Pouzolzia indica methanolic extract fraction 3 at a<br />
concentration <strong>of</strong> 1:8 and by Virkon® solution at a concentration <strong>of</strong> 0.25%, with a minimal cysticidal concentration (MCC) by<br />
24 hours. Both agents caused similar structural damage to Acanthamoeba cysts in the same sequence. Step by step structural<br />
alterations occurred within the cyst. First, the cyst shrank, collapsed and had clumping <strong>of</strong> cytoplasmic stuctures inside the<br />
cyst walls. Second, the cysts began to bulge, swell, have a decrease in wrinkles in the cyst walls and spill the cytoplasmic<br />
contents into the environment. Finally, the cyst walls broke into small pieces. This study may be beneficial to compare with<br />
future studies <strong>of</strong> pharmaceutical agents against Acanthamoeba keratitis.<br />
No.1161<br />
Author (s) : Roop-Ngam, P., Thongboonkerd, V.<br />
Title : Development <strong>of</strong> an oxalate-affinity chromatographic column to isolate oxalate-binding proteins<br />
Source : Analytical Methods, Volume 2, Issue 8, August <strong>2010</strong>, Pages 1051-1055<br />
Document Type : Article<br />
Keywords : Affinity column, Amino group, Binding proteins, Calcium binding proteins, Calcium oxalates, Carbodiimide<br />
hydrochlorides, Carbodiimides, Chromatographic column, Crystalline components, High efficiency, Kidney<br />
stone, Ninhydrin, Oxalic Acid, Sepharose 4B, SIMPLE method, Specific binding; Carbonic anhydrase, Enzyme inhibition, Gel<br />
permeation chromatography, Organic acids; Proteins<br />
<strong>Abstract</strong> : Calcium oxalate (CaOx) is the major crystalline component in kidney stones. Many previous studies on stone modulation<br />
have focused mainly to calcium-binding proteins, whereas oxalate-binding proteins remain under-investigated due to a lack <strong>of</strong><br />
a simple method to purify oxalate-binding proteins. Therefore, we have developed an affinity chromatographic column to<br />
isolate or purify oxalate-binding proteins. The oxalate-affinity column developed contained EAH-Sepharose 4B beads<br />
conjugated with oxalic acid by carbodiimide reaction using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride<br />
(EDC) as an activator. The coupling efficacy <strong>of</strong> coupled beads (oxalic acid-EAH Sepharose 4B) was determined by a<br />
competitive ninhydrin assay to quantify the remaining amino group on EAH-Sepharose 4B. In addition, the oxalate-affinity<br />
column showed high specific binding to a known oxalate-binding protein (p62) but not to an irrelevant protein (carbonic<br />
anhydrase). In conclusion, we have established the oxalate-affinity chromatographic column, which showed the high<br />
efficiency to isolate oxalate-binding proteins. This novel chromatographic column will be very useful for future stone<br />
research, particularly in the area <strong>of</strong> stone modulation by oxalate-binding proteins. © <strong>2010</strong> The Royal Society <strong>of</strong> Chemistry.<br />
No.1162<br />
Author (s) : Rooppakhun, S., Chantarapanich, N., Chernchujit, B., Mahaisavariya, B., Sucharitpwatskul, S., Sitthiseripratip, K.<br />
Title : Mechanical evaluation <strong>of</strong> stainless steel and titanium dynamic hip screws for trochanteric fracture<br />
Source : Proceedings <strong>of</strong> World Academy <strong>of</strong> Science, Engineering and Technology, Volume 70, September <strong>2010</strong>, Pages 662-665<br />
Document Type : Article<br />
Keywords : Dynamic hip screw (DHS); Finite element analysis; Trochanteric fracture<br />
<strong>Abstract</strong> : This study aimed to present the mechanical performance evaluation <strong>of</strong> the dynamic hip screw (DHS) for trochanteric fracture<br />
by means <strong>of</strong> finite element method. The analyses were performed based on stainless steel and titanium implant material<br />
definitions at various stages <strong>of</strong> bone healing and including implant removal. The assessment <strong>of</strong> the mechanical performance<br />
used two parameters, von Mises stress to evaluate the strength <strong>of</strong> bone and implant and elastic strain to evaluate fracture<br />
stability. The results show several critical aspects <strong>of</strong> dynamic hip screw for trochanteric fracture stabilization. In the initial<br />
stage <strong>of</strong> bone healing process, partial weight bearing should be applied to avoid the implant failure. In the late stage <strong>of</strong> bone<br />
healing, stainless steel implant should be removed.<br />
No.1163<br />
Author (s) : Rosenfeld, L., Reddi, A.R., Leung, E., Aranda, K., Jensen, L.T., Culotta, V.C.<br />
Title : The effect <strong>of</strong> phosphate accumulation on metal ion homeostasis in Saccharomyces cerevisiae<br />
Source : Journal <strong>of</strong> Biological Inorganic Chemistry, Volume 15, Issue 7, September <strong>2010</strong>, Pages 1051-1062<br />
Document Type : Article<br />
Keywords : Aft1; Manganese; Pho80; Polyphosphate; Yeast<br />
<strong>Abstract</strong> : Much <strong>of</strong> what is currently understood about the cell biology <strong>of</strong> metals involves their interactions with proteins. By<br />
comparison, little is known about interactions <strong>of</strong> metals with intracellular inorganic compounds such as phosphate. Here we<br />
examined the role <strong>of</strong> phosphate in metal metabolism in vivo by genetically perturbing the phosphate content <strong>of</strong><br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Saccharomyces cerevisiae cells. Yeast pho80 mutants cannot sense phosphate and have lost control <strong>of</strong> phosphate uptake,<br />
storage, and metabolism. We report here that pho80 mutants specifically elevate cytosolic and nonvacuolar levels <strong>of</strong><br />
phosphate and this in turn causes a wide range <strong>of</strong> metal homeostasis defects. Intracellular levels <strong>of</strong> the hard-metal cations<br />
sodium and calcium increase dramatically, and cells become susceptible to toxicity from the transition metals manganese,<br />
cobalt, zinc, and copper. Disruptions in phosphate control also elicit an iron starvation response, as pho80 mutants were seen<br />
to upregulate iron transport genes. The iron-responsive transcription factor Aft1p appears activated in cells with high<br />
phosphate content in spite <strong>of</strong> normal intracellular iron levels. The high phosphate content <strong>of</strong> pho80 mutants can be lowered<br />
by mutating Pho4p, the transcription factor for phosphate uptake and storage genes. Such lowering <strong>of</strong> phosphate content by<br />
pho4 mutations reversed the high calcium and sodium content <strong>of</strong> pho80 mutants and prevented the iron starvation<br />
response. However, pho4 mutations only partially reversed toxicity from heavy metals, representing a novel outcome <strong>of</strong><br />
phosphate dysregulation. Overall, these studies underscore the importance <strong>of</strong> maintaining a charge balance in the cell; a<br />
disruption in phosphate metabolism can dramatically impact on metal homeostasis. © <strong>2010</strong> SBIC.<br />
No.1164<br />
Author (s) : Rotkittikhun, P., Kruatrachue, M., Pokethitiyook, P., Baker, A.J.M.<br />
Title : Tolerance and accumulation <strong>of</strong> lead in Vetiveria zizanioides and its effect on oil production<br />
Source : Journal <strong>of</strong> Environmental Biology, Volume 31, Issue 3, May <strong>2010</strong>, Pages 329-334<br />
Document Type : Article<br />
Keywords : Accumulation; Lead; Tolerance; Vetiver oil; Vetiveria zizanioides<br />
<strong>Abstract</strong> : Experiments were conducted to evaluate lead tolerance and accumulation in vetiver grass Vetiveria zizanioides (L), grown in<br />
hydroponics and a pot study and to examine the effect <strong>of</strong> lead on vetiver oil production. Elevated concentrations <strong>of</strong> lead<br />
decreased the length <strong>of</strong> shoots and roots <strong>of</strong> plants. However, vetiver grown in highly contaminated soils showed no apparent<br />
phytotoxicity symptoms. Lead concentrations in the shoots and roots <strong>of</strong> vetiver plants grown in hydroponics were up to 144<br />
and 19530 mg kg-1 and those grown in soil were 38 and 629 mg kg-1, respectively. Lead had an effect on vetiver oil<br />
production and composition by stimulating oil yield and the number <strong>of</strong> its constituents. Oil yield ranged from 0.4-1.3%; the<br />
highest yields were found in plants grown in nutrient solution with 100 mg Pb l -1 for 5 weeks (1.29%) and 7 weeks (1.22%).<br />
The number <strong>of</strong> total constituents <strong>of</strong> vetiver oil also variedbetween 47-143 compounds when lead was present in the growth<br />
medium. The highest number (143) was found in plants grown in soil spiked with 1000 mg Pb kg-1. The predominant<br />
compound was khusimol (10.7-18.1%) followed by (E)-isovalencenol (10.3-15.6%). Our results indicated that lead could<br />
increase the oil production <strong>of</strong> vetiver. © Triveni Enterprises, Lucknow (India).<br />
No.1165<br />
Author (s) : Rottmann, M., McNamara, C., Yeung, B.K.S., Lee, M.C.S., Zou, B., Russell, B., Seitz, P., Plouffe, D.M., Dharia, N.V., Tan, J., Cohen,<br />
S.B., Spencer, K.R., González-Páez, G.E., Lakshminarayana, S.B., Goh, A., Suwanarusk, R., Jegla, T., Schmitt, E.K., Beck, H.-P., Brun,<br />
R., Nosten, F., Renia, L., Dartois, V., Keller, T.H., Fidock, D.A., Winzeler, E.A., Diagana, T.T.<br />
Title : Spiroindolones, a potent compound class for the treatment <strong>of</strong> malaria<br />
Source : Science, Volume 329, Issue 5996, 3 September <strong>2010</strong>, Pages 1175-1180<br />
Document Type : Article<br />
Keywords : adenosine triphosphatase, antimalarial agent, artemether, artesunate, chloroquine, mefloquine, nitd 609, p type cation<br />
transporter atpase4, spiroindolone derivative, unclassified drug; antimalarial agent, blood, cation, drug, enzyme<br />
activity, experimental study, malaria, protein, rodent, tolerance; animal experiment, animal model, antimalarial activity, area<br />
under the curve, article, cell proliferation, concentration response, controlled study, cytotoxicity, drug bioavailability, drug<br />
clearance, drug distribution, drug efficacy, drug half life, drug metabolism, drug safety, drug structure, drug synthesis, drug<br />
tolerability, gene mutation, malaria, male, mouse, nonhuman, Plasmodium falciparum, Plasmodium vivax, priority<br />
journal, protein synthesis, rat, screening, survival rate; Adenosine Triphosphatases, Animals, Antimalarials, Cell Line, Drug<br />
Discovery, Drug Resistance, Erythrocytes, Female, Genes, Protozoan, Humans, Indoles, Malaria, Male, Mice, Models,<br />
Molecular, Mutant Proteins, Mutation, Parasitic Sensitivity Tests, Plasmodium berghei, Plasmodium falciparum, Plasmodium<br />
vivax, Protein Synthesis Inhibitors, Protozoan Proteins, Rats, Rats, Wistar, Spiro Compounds; Plasmodium<br />
falciparum, Plasmodium vivax, Rodentia<br />
<strong>Abstract</strong> : Recent reports <strong>of</strong> increased tolerance to artemisinin derivatives - the most recently adopted class <strong>of</strong> antimalarials - have<br />
prompted a need for new treatments. The spirotetrahydro-β-carbolines, or spiroindolones, are potent drugs that kill the<br />
blood stages <strong>of</strong> Plasmodium falciparum and Plasmodium vivax clinical isolates at low nanomolar concentration.<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Spiroindolones rapidly inhibit protein synthesis in P. falciparum, an effect that is ablated in parasites bearing nonsynonymous<br />
mutations in the gene encoding the P-type cation-transporter ATPase4 (PfATP4). The optimized spiroindolone NITD609 shows<br />
pharmacokinetic properties compatible with once-daily oral dosing and has single-dose efficacy in a rodent malaria model.<br />
No.1166<br />
Author (s) : Roubsanthisuk, W., Wongsurin, U., Buranakitjaroen, P.<br />
Title : Hypertensive emergencies remain a clinical problem and are associated with high mortality.<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand = Chotmaihet thangphaet, Volume 93 Suppl 1, January <strong>2010</strong>, Pages S54-61<br />
Document Type : Article<br />
Keywords : antihypertensive agent; adult, aged, article, blood pressure, cause <strong>of</strong> death, chronic kidney failure, diabetes<br />
mellitus, emergency, female, follow up, human, hypertension, length <strong>of</strong> stay, male, middle aged, mortality, risk factor, stroke;<br />
Adult, Aged, Aged, 80 and over, Antihypertensive Agents, Blood Pressure, Cause <strong>of</strong> Death, Diabetes<br />
Complications, Emergencies, Female, Follow-Up Studies, Hospital Mortality, Humans, Hypertension, Kidney Failure,<br />
Chronic, Length <strong>of</strong> Stay, Male, Middle Aged, Risk Factors, Stroke, Young Adult<br />
<strong>Abstract</strong> : OBJECTIVE: We suspect that hypertensive emergencies remain a clinical problem and data on their long-term prognosis are<br />
lacking. This study was conducted in order to determine the frequency, management, and outcome <strong>of</strong> hypertensive<br />
emergencies in this era, in which hypertension treatment is more effective than in the past. MATERIAL AND METHOD: We<br />
reviewed the medical records <strong>of</strong> patients with hypertensive emergencies admitted to the medical wards <strong>of</strong> Siriraj Hospital in<br />
2003 and collected data on their characteristic, management, investigations, and follow-ups through 31 December 2007.<br />
RESULTS: There were 184 patients included. Hypertension has been previously diagnosed in 89% <strong>of</strong> cases. Nearly half also<br />
had diabetes mellitus and around a quarter had chronic kidney failure. Mean +/- SD <strong>of</strong> blood pressure at presentation was<br />
205.96 +/- 21.36/114.60 +/- 20.59 mmHg. Cardiac complications and stroke accounted for 71% and 23% <strong>of</strong> all target organ<br />
damage, respectively. Intravenous nitroglycerine and furosemide were most frequently prescribed. Additional investigations to<br />
search for the causes <strong>of</strong> hypertension were performed in only 55 cases. The average hospital stay was 9.8 days. The inhospital<br />
mortality rate was 15%. Some 26% <strong>of</strong> patients were lost to follow-up and another 20% died later. Only 19% <strong>of</strong><br />
patients had regular follow-ups until the end <strong>of</strong> 2007 and remained on an average <strong>of</strong> 2.4 antihypertensive drugs.<br />
CONCLUSION: Hypertensive emergencies are usually found in patients with a history <strong>of</strong> hypertension and diabetes mellitus or<br />
kidney failure. Recommended investigations usually failed to identify the cause <strong>of</strong> hypertension. The mortality rate <strong>of</strong> these<br />
patients was extremely high while their adherence to treatment was extremely poor.<br />
No.1167<br />
Author (s) : Round, P.D.<br />
Title : An analysis <strong>of</strong> records <strong>of</strong> three passage migrants in Thailand: Tiger Shrike Lantus tigrinus, Yellow-rumped Flycatcher Ficedula<br />
zanthopygia and Mugimaki Flycatcher Emugimaki<br />
Source : Forktail, Issue 26, August <strong>2010</strong>, Pages 24-30<br />
Document Type : Article<br />
Keywords : Aves, Ficedula zanthopygia, Laniidae, Lanius tigrinus<br />
<strong>Abstract</strong> : Records <strong>of</strong> three passage migrants in Thailand are analysed to show seasonal and geographical differences in their<br />
distribution. While two <strong>of</strong> these, Tiger Shrike Lanius tigrinus and Yellow-rumped Flycatcher Ficedula zanthopygia, are early<br />
autumn migrants, the third species, Mugimaki Flycatcher F. mugimaki, is a much later autumn migrant and, in contrast to the<br />
other two, comprises birds that winter in Thailand as well as passage migrant individuals that winter beyond the country's<br />
southern border. Disproportionately more Mugimaki Flycatchers are recorded on spring migration than the other two species.<br />
Definitive-plumaged males <strong>of</strong> both flycatcher species were recorded earlier on spring and autumn migration than others<br />
(grouped female or immature birds). Although in recent decades the numbers <strong>of</strong> migrants reported have increased due to<br />
increased ornithological recording, the numbers <strong>of</strong> Tiger Shrikes have not increased in proportion to those <strong>of</strong> Yellow-rumped<br />
Flycatchers, possibly indicating a decline in numbers.<br />
No.1168<br />
Author (s) : Round, P.D., Allen, D.<br />
Title : A record <strong>of</strong> active moult in the Streaked Reed Warbler Acrocephalus sorghophilus<br />
Source : Bulletin <strong>of</strong> the British Ornithologists' Club, Volume 130, Issue 2, 14 June <strong>2010</strong>, Pages 145-147<br />
Document Type : Article<br />
425
Keywords : Acrocephalus; Acrocephalus sorghophilus<br />
<strong>Abstract</strong> : –<br />
No.1169<br />
Author (s) : Round, P.D., Leader, P.J., Waengsothorn, S.<br />
Title : Removal <strong>of</strong> Pink-rumped Rosefinch Carpodacus eos from the Thai avifaunal list<br />
Source : Forktail, Issue 26, August <strong>2010</strong>, Pages 145-147<br />
Document Type : Article<br />
Keywords : Carpodacus eos<br />
<strong>Abstract</strong> : –<br />
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.1170<br />
Author (s) : Ruamsup, S., Wiratkapun, C., Wibulpolprasert, B., Lertsithichai, P.<br />
Title : A comparison between short-interval and regular-interval follow-up for BI-RADS category 3 lesions<br />
Source : Singapore Medical Journal, Volume 51, Issue 2, February <strong>2010</strong>, Pages 120-125<br />
Document Type : Article<br />
Keywords : Bi-RADS category 3; Breast cancer; Regular-interval follow-up; Screen imaging studies; Short-interval follow-up<br />
<strong>Abstract</strong> : Introduction: The aim <strong>of</strong> this study was to compare the short-interval and regular-interval follow-up in women with Breast<br />
Imaging Reporting and Data System (BI-RADS) category 3 screen imaging studies. The image stability, rate <strong>of</strong> image -detected<br />
breast cancer and stage <strong>of</strong> cancer are studied. Methods: Women who had BI-RADS 3 screen imaging studies (mammography<br />
and ultrasonography) conducted between the period January 2003 and December 2005 were retrospectively identified using<br />
the computerised database at the Department <strong>of</strong> Radiology, Ramathibodi Hospital, Thailand. Women who had known breast<br />
cancer status at two years after screening were included in the study and divided into two groups: short-interval (six months<br />
after screening) or regular-interval (one year after screening) follow-up. The two groups were compared in terms <strong>of</strong> the<br />
baseline clinico- radiologic characteristics and outcomes, including the image stability, image-detected breast cancer and the<br />
cancer stage at detection. Results: A total <strong>of</strong> 10,086 women underwent screen imaging studies within the study period. Of<br />
these, 1,541 (15 percent) were categorised as BI-RADS 3. Only 1,036 women (67 percent) had follow-up images done six to 12<br />
months after screening, and 846 (82 percent <strong>of</strong> 1,036 women) also had known cancer status two years after the screening.<br />
Breast cancer was noted in seven women (a positive predictive value <strong>of</strong> 0.7 percent). There were no significant differences<br />
between the two groups <strong>of</strong> women in terms <strong>of</strong> their baseline characteristics, image stability at the initial follow-up, the rate <strong>of</strong><br />
image-detected breast cancer and the stage <strong>of</strong> cancer at detection. Conclusion: There were no significant differences in the<br />
effectiveness <strong>of</strong> short-interval versus regular-interval follow-up in women with BI- RADS 3 screen imaging studies.<br />
No.1171<br />
Author (s) : Ruamviboonsuk, P., Tadarati, M., Vanichvaranont, S., Hanutsaha, P., Pokawattana, N.<br />
Title : Photodynamic therapy combined with ranibizumab for polypoidal choroidal vasculopathy: Results <strong>of</strong> a 1-year preliminary<br />
study<br />
Source : British Journal <strong>of</strong> Ophthalmology, Volume 94, Issue 8, August <strong>2010</strong>, Pages 1045-1051<br />
Document Type : Article<br />
Keywords : ranibizumab; adult, aged, article, clinical article, clinical trial, female, human, indocyanine green angiography, male, optical<br />
coherence tomography, photodynamic therapy, polyp, polypoidal choroidal vasculopathy, priority journal, retina fluorescein<br />
angiography, retina hemorrhage, treatment outcome, visual acuity; Aged, Aged, 80 and over, Angiogenesis<br />
Inhibitors, Antibodies, Monoclonal, Choroidal Neovascularization, Combined Modality Therapy, Diagnostic Techniques,<br />
Ophthalmological, Female, Humans, Male, Middle Aged, Photochemotherapy, Photosensitizing Agents, Porphyrins, Prospective<br />
Studies, Retinal Hemorrhage, Treatment Outcome, Visual Acuity<br />
<strong>Abstract</strong> : Background/aims: To determine the potential efficacy and safety <strong>of</strong> combined verteporfin photodynamic therapy (PDT) with<br />
ranibizumab for the treatment <strong>of</strong> polypoidal choroidal vasculopathy (PCV). Methods: In this prospective, non-comparative,<br />
interventional study, 12 eyes from 12 patients that had active PCV were treated with PDT combined with three monthly<br />
intravitreal injections <strong>of</strong> ranibizumab. The patients were then monitored monthly with measurements <strong>of</strong> best-corrected visual<br />
acuity (BCVA) and central retinal thickness quantified by optical coherence tomography for 1 year. Indocyanine green<br />
angiography (ICGA) and fluorescein angiography were performed every 3 months. The eyes were re-treated with PDT and a<br />
ranibizumab injection, or with solely ranibizumab injection when indicated. Results: At month 12, the mean BCVA change<br />
426
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
from baseline was +12.3 letters (p=0.04). Eight patients (58.3%, p=0.02) had a BCVA gain <strong>of</strong> 15 letters or more. One patient<br />
(8.3%, p=1.0) had a BCVA loss <strong>of</strong> 15 letters or more. All patients underwent regression <strong>of</strong> polyps without recurrence. One<br />
patient experienced an insignificant subretinal haemorrhage. No other adverse event that could be attributed to the<br />
treatment was observed. Conclusions: This combination therapy showed encouraging results concerning improving vision,<br />
reducing the incidence <strong>of</strong> subretinal haemorrhage and reducing the recurrence <strong>of</strong> polyps when compared to previously<br />
published data that reported PDT monotherapy for PCV.<br />
No.1172<br />
Author (s) : Ruangsetakit, C., Chinsakchai, K., Mahawongkajit, P., Wongwanit, C., Mutirangura, P.<br />
Title : Transcutaneous oxygen tension: A useful predictor <strong>of</strong> ulcer healing in critical limb ischaemia<br />
Source : Journal <strong>of</strong> Wound Care, Volume 19, Issue 5, May <strong>2010</strong>, Pages 202-206<br />
Document Type : Article<br />
Keywords : Critical limb ischaemia; Transcutaneous oxygen tension; Ulcer healing<br />
<strong>Abstract</strong> : Objective: To investigate the threshold <strong>of</strong> transcutaneous oxygen tension (TcPO2) values in predicting ulcer healing in patients<br />
with critical limb ischaemia in a prospective study. Method: 50 patients suffering from critical limb ischaemia with chronic<br />
ischemic ulcers or gangrenous toes were enrolled in this study between January and December 2008. Their demographic data<br />
and ankle brachial pressure index (ABPI) were collected. Baseline ulcers were measured with a wound measurement system<br />
(Visitrak, Smith & Nephew). TcPO2 was measured at rest in the supine position and with 30° leg elevation. The patients<br />
with infective and ischemic ulcers underwent debridement and gangrenous toes were amputated. Ulcer outcome was classifi<br />
ed as either: (1) A healing ulcer, showing good epithelialisation or granulation at both base and edges, or a decrease in ulcer<br />
area during the study; or (2) A non-healing ulcer, showing poor granulation tissue formation or a pale base and necrotic edges,<br />
or deterioration in an ischaemic ulcer. Results: The mean age <strong>of</strong> the patients was 67.6 ± 10.8 years. The most common risk<br />
factor was hypertension (90%). Mean ABPI was 0.75 ± 0.39. 13 patients (26%) had a TcPO2 <strong>of</strong> less than 20mmHg, <strong>of</strong> which<br />
none showed any improvement in ulcer healing (p<0.001). 15 patients (30%) had a TcPO2 <strong>of</strong> more than 40mmHg, <strong>of</strong> which<br />
all progressed to complete ulcer healing (p<0.001). In the borderline group (20-40mmHg, 22 patients, 44%), 10 patients<br />
(45%) had a TcPO2 drop <strong>of</strong> ≤10mmHg with 30° leg elevation, <strong>of</strong> which 8 achieved complete ulcer healing (p<0.001). 12<br />
patients (55%) had a TcPO 2 drop <strong>of</strong> >10mmHg with 30° leg elevation, <strong>of</strong> which 11 showed no ulcer healing (p<0.001).<br />
Conclusion: TcPO2 measurement is an accurate, non-invasive, and good predictor <strong>of</strong> ischemic ulcer healing, for cut-<strong>of</strong>f TcPO2<br />
values <strong>of</strong> less than 20mmHg and more than 40mmHg. In addition, the leg elevation method for TcPO2 might provide an<br />
important adjunct in the assessment <strong>of</strong> patients with borderline values. Declaration <strong>of</strong> interest: None.<br />
No.1173<br />
Author (s) : Ruangvutilert, P., Chaemsaithong, P., Ruangrongmorakot, K., Kanokpongsakdi, S., Sunsaneevithayakul, P.<br />
Title : Development <strong>of</strong> a modified 100-gram oral glucose tolerance test for diagnosis <strong>of</strong> gestational diabetes mellitus and its<br />
diagnostic accuracy<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 10, October <strong>2010</strong>, Pages 1121-1127<br />
Document Type : Article<br />
Keywords : Gestational diabetes mellitus; Modified oral glucose tolerance test<br />
<strong>Abstract</strong> : Objective: To develop a modified 100-g oral glucose tolerance test (100-g OGTT) for the diagnosis <strong>of</strong> gestational diabetes<br />
mellitus (GDM) in order to reduce the number <strong>of</strong> values needed for the test. Materials and Methods: Patient charts <strong>of</strong><br />
pregnant women who completed the 100-g OGTT test at the antenatal clinic, Siriraj Hospital between 2005 and 2006 were<br />
reviewed. Cases diagnosed with GDM using standard 100-g OGTT were selected. In non-GDM cases, the last 100-g OGTT test<br />
was selected. Diagnostic performances <strong>of</strong> each glucose value and two or three values in various combinations were<br />
determined. Results: One thousand seven hundred sixty three women completed GDM diagnosis throughout their pregnancy.<br />
Four hundred three women had GDM while 1,360 subjects were non-GDM. Considering single glucose values, the highest level<br />
<strong>of</strong> accuracy, and the best ROC curve were obtained from the value at 2 hours after glucose ingestion (2-h glucose value) with<br />
93.00% accuracy and the area under the ROC curve <strong>of</strong> 0.961. The combination <strong>of</strong> 2-h glucose value with fasting plasma<br />
glucose (FPG) showed 93.25% accuracy. FPG combined with 1-h and 2-h glucose values achieved 100% sensitivity with 92%<br />
accuracy. Conclusion: FPG in combined with 1-h and 2-h glucose values is an interesting alternative for the diagnosis <strong>of</strong> GDM.<br />
No.1174<br />
Author (s) : Ruangwises, N., Ruangwises, S.<br />
427
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Aflatoxin M1 contamination in raw milk within the central region <strong>of</strong> Thailand<br />
Source : Bulletin <strong>of</strong> Environmental Contamination and Toxicology, Volume 85, Issue 2, August <strong>2010</strong>, Pages 195-198<br />
Document Type : Article<br />
Keywords : Aflatoxin M1; HPLC; Immunoaffinity column; Raw milk<br />
<strong>Abstract</strong> : Aflatoxin M1 (AFM1) was found in all <strong>of</strong> the 240 raw milk samples collected from milk tanks <strong>of</strong> 80 dairy farms at a collecting<br />
center in the central region <strong>of</strong> Thailand. Milk samples from individual farms were collected in three seasons. The average<br />
concentration <strong>of</strong> AFM1 in milk samples collected in winter (0.089 ± 0.034 μg/L) was significantly higher than those in rainy<br />
season (0.071 ± 0.028 μg/L) and summer (0.050 ± 0.021 μg/L). The present study suggests that regulatory limits for AFM1 are<br />
needed to regulate and ensure the quality <strong>of</strong> raw milk and milk products in Thailand. © <strong>2010</strong> Springer Science+Business<br />
Media, LLC.<br />
No.1175<br />
Author (s) : Ruchirawat, M., Navasumrit, P., Settachan, D.<br />
Title : Exposure to benzene in various susceptible populations: Co-exposures to 1,3-butadiene and PAHs and implications for<br />
carcinogenic risk<br />
Source : Chemico-Biological Interactions, Volume 184, Issue 1-2, March <strong>2010</strong>, Pages 67-76<br />
Document Type : Article<br />
Keywords : 1,3-Butadiene; Benzene; Correlation; DNA damage; DNA repair capacity; PAHs<br />
<strong>Abstract</strong> : Exposure to benzene in human populations can occur in various work-related settings in which benzene is used or produced,<br />
or from traffic emissions resulting from incomplete combustion <strong>of</strong> fossil fuel, or from other sources. Two scenarios <strong>of</strong><br />
benzene exposure were studied in 4 susceptible groups in Thailand. The first scenario is work-related exposures primarily to<br />
benzene, with the study subjects consisting <strong>of</strong> petrochemical laboratory workers and gasoline service station attendants, who<br />
are exposed at levels <strong>of</strong> 78.32 and 360.84μg/m3, respectively. The second scenario is traffic-related exposure and exposure<br />
to incense smoke, where co-exposures to other pollutants occurs, with the study groups consisting <strong>of</strong> school children<br />
attending schools in the city center and exposed to traffic emissions, and temple workers exposed to incense smoke. The<br />
individual benzene exposure levels were approximately 19.38μg/m3 in city school children and 45.90μg/m3 in temple<br />
workers. Co-exposures to 1,3-butadiene and polycyclic aromatic hydrocarbons (PAHs) generated from the same sources<br />
occurred in the second exposure scenario. 8-OHdG, DNA strand breaks and DNA repair capacity were measured as biomarkers<br />
<strong>of</strong> early effects <strong>of</strong> carcinogenic compound exposure. Petrochemical laboratory workers and gasoline service stations<br />
attendants had significantly higher levels <strong>of</strong> DNA strand breaks and significantly lower DNA repair capacity compared to<br />
controls, while gasoline service station attendants also had significantly higher levels <strong>of</strong> 8-OHdG than controls. City school<br />
children had significantly higher levels <strong>of</strong> PAH-DNA adducts, 8-OHdG, and DNA strand breaks and significantly lower levels <strong>of</strong><br />
DNA repair capacity compared to rural children. Temple workers also had significantly higher levels <strong>of</strong> 8-OHdG and DNA<br />
strand breaks and significantly lower levels <strong>of</strong> DNA repair capacity compared to controls. In all <strong>of</strong> the study groups, the levels<br />
<strong>of</strong> benzene exposure correlated significantly with 8-OHdG levels, DNA strand breaks, and DNA repair capacity. In school<br />
children, PAH levels also correlated significantly with 8-OHdG levels, DNA strand breaks and DNA repair capacity. In temple<br />
workers, 1,3-butadiene levels correlated significantly with 8-OHdG and DNA strand breaks, but not with DNA repair capacity,<br />
while in the school children they did not correlate significantly with 8-OHdG or DNA strand breaks, and correlated marginally<br />
significantly with DNA repair capacity (deletions per metaphase). Multivariate regression analysis identified total PAHs<br />
concentrations converted to B[a]P equivalents as the only factor significantly affecting 8-OHdG levels, and total PAHs<br />
concentrations converted to B[a]P equivalents, as well as 1,3-butadiene concentrations as the factors significantly affecting<br />
DNA repair capacity in the school children. PAHs concentration was identified as the factor most significantly affecting DNA<br />
strand breaks in temple workers, followed by benzene concentrations, while DNA repair capacity was also significantly<br />
influenced by PAHs concentrations. © <strong>2010</strong> Elsevier Ireland Ltd.<br />
No.1176<br />
Author (s) : Rudge, J.W., Phuanakoonon, S., Nema, K.H., Mounier-Jack, S., Coker, R.<br />
Title : Critical interactions between Global Fund-supported programmes and health systems: a case study in Papua New Guinea.<br />
Source : Health policy and planning, Volume 25 Suppl 1, November <strong>2010</strong>, Pages i48-52<br />
Document Type : Article<br />
Keywords : article, economics, female, financial management, government, health care delivery, health services<br />
research, human, infection control, international cooperation, interview, male, organization and management, Papua New<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Guinea, system analysis; Communicable Disease Control, Delivery <strong>of</strong> Health Care, Female, Financing,<br />
Government, Government Programs, Humans, <strong>International</strong> Cooperation, Interviews as Topic, Male, Organizational Case<br />
Studies, Papua New Guinea, Systems Integration<br />
<strong>Abstract</strong> : In Papua New Guinea, investment by the Global Fund to Fight AIDS, Tuberculosis and Malaria (the Global Fund) has played an<br />
important role in scaling up the response to HIV and tuberculosis (TB). As part <strong>of</strong> a series <strong>of</strong> case studies on how Global Fundsupported<br />
programmes interact with national health systems, we assessed the nature and extent <strong>of</strong> integration <strong>of</strong> the Global<br />
Fund portfolios within the national HIV and TB programmes, the integration <strong>of</strong> the HIV and TB programmes within the general<br />
health system, and system-wide effects <strong>of</strong> Global Fund support in Papua New Guinea. The study relied on a literature review<br />
and 30 interviews with key stakeholders using the Systemic Rapid Assessment Toolkit and thematic analysis. Global Fundsupported<br />
activities were found to be largely integrated, or at least coordinated, with the national HIV and TB programmes.<br />
However, this has reinforced the vertical nature <strong>of</strong> these programmes with respect to the general health system, with parallel<br />
systems established to meet the demands <strong>of</strong> programme scale-up and the performance-based nature <strong>of</strong> Global Fund<br />
investment in the weak health system context <strong>of</strong> Papua New Guinea. The more parallel functions include monitoring and<br />
evaluation, and procurement and supply chain systems, while human resources and infrastructure for service delivery are<br />
increasingly integrated at more local levels. Positive synergies <strong>of</strong> Global Fund support include engagement <strong>of</strong> civil-society<br />
partners, and a reliable supply <strong>of</strong> high-quality drugs which may have increased patient confidence in the health system.<br />
However, the severely limited and overburdened pool <strong>of</strong> human resources has been skewed towards the three diseases,<br />
both at management and service delivery levels. There is also concern surrounding the sustainability <strong>of</strong> the disease<br />
programmes, given their dependence on donors. Increasing Global Fund attention towards health system strengthening was<br />
viewed positively, but should acknowledge that system changes are slow, difficult to measure and require long-term support.<br />
No.1177<br />
Author (s) : Rujkorakarn, R., Nunthaboot, N., Tanaka, F., Chaiyen, P., Chosrowjan, H., Taniguchi, S., Mataga, N.<br />
Title : Time-resolved Stokes shift in proteins with continuum model: Slow dynamics in proteins<br />
Source : Journal <strong>of</strong> Photochemistry and Photobiology A: Chemistry, Volume 215, Issue 1, 5 September <strong>2010</strong>, Pages 38-45<br />
Document Type : Article<br />
Keywords : C1 protein; Continuum model; Flavin; Protein; Theoretical analyses; Time-resolved Stokes shift; Tryptophan<br />
<strong>Abstract</strong> : Reported time-resolved Stokes shifts (TRSS) <strong>of</strong> free tryptophan (Trp) and free p-coumaric acid (CA) in water, and Trp in<br />
monellin, apomyoglobin, and isoalloxazine (Iso) <strong>of</strong> flavin mononucleotide (FMN) in the reductase component (C1 protein) <strong>of</strong><br />
p-hydroxyphenylacetate hydroxylase were analyzed with continuum model. All unknown parameters <strong>of</strong> these systems in the<br />
theoretical equations were determined to obtain the best fit between the observed and calculated TRSS, according to a nonlinear<br />
least square method. TRSS <strong>of</strong> free Trp at 295 K was also analyzed with four sets <strong>of</strong> reported dielectric constants and<br />
solvent relaxation times <strong>of</strong> water. Agreement between the observed and calculated TRSS <strong>of</strong> the free Trp was excellent. In CA<br />
the calculated TRSS could satisfactorily reproduce the observed one. Frequency-dependent dielectric constants <strong>of</strong> Trp in the<br />
proteins and Iso in C1 protein were expressed with 2- and 3-relaxation times. Static dielectric constant, ε0, intermediate<br />
permittivity, ε1, dielectric constant <strong>of</strong> Iso, εc, 2-relaxation times, τ1 and τ2, μe and D0 in the 2-relaxation time analyses were<br />
determined by the best-fit procedures. Agreements between the observed and calculated TRSS <strong>of</strong> Trp in native, denatured<br />
monellins, apomyoglobin, and Iso in C1 protein were excellent. No further improvements were obtained with 3-relaxation<br />
time analyses. Origin <strong>of</strong> the slow decaying component <strong>of</strong> TRSS in apomyoglobin was interpreted with continuum model and<br />
compared with molecular dynamics (MD) simulation model and a continuum model by Halle and Nilsson [J. Phys. Chem. B<br />
113 (2009) 8210]. Frozen states revealed with MD model were reproduced with the 3-relaxation time analysis. © <strong>2010</strong> Elsevier<br />
B.V.<br />
No.1178<br />
Author (s) : Ruksee, N.; Govitrapong, P.<br />
Title : THE EFFECT OF MELATONIN ON NR2A/B AND BDNF IN DEXAMETHASONE-INDUCED CHRONIC STRESS OF ADULT MICE<br />
HIPPOCAMPAL BRAIN AREA<br />
Source : JOURNAL OF NEUROCHEMISTRY, Volume: 115, Supplement: 1, Pages: 77-77, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
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No.1179<br />
Author (s) : Rung-Arunwan, T., Siripunvaraporn, W.<br />
Title : An efficient modified hierarchical domain decomposition for two-dimensional magnetotelluric forward modeling<br />
Source : Geophysical Journal <strong>International</strong>, Volume 183, Issue 2, November <strong>2010</strong>, Pages 634-644<br />
Document Type : Article<br />
Keywords : Electromagnetic theory; Inverse theory; Magnetotelluric; Numerical approximations and analysis; Numerical solutions<br />
<strong>Abstract</strong> : We use 2-D magnetotelluric (MT) problems as a feasibility study to demonstrate that 3-D MT problems can be solved with a<br />
direct solver, even on a standard single processor PC. The scheme used is a hierarchical domain decomposition (HDD)<br />
method in which a global computational domain is uniformly split into many smaller non-overlapping subdomains. To make<br />
it more efficient, two modifications are made to the standard HDD method. Instead <strong>of</strong> three levels as in the standard HDD<br />
method, we classify the unknowns into four classes: the interiors, the horizontal interfaces, the vertical interfaces and the<br />
intersections. Four sets <strong>of</strong> smaller systems <strong>of</strong> equations are successively solved with a direct method (an LU factorization).<br />
The separation significantly reduces the large memory requirements <strong>of</strong> a direct solver. It also reduces the CPU time to almost<br />
half that <strong>of</strong> the standard HDD method although it is still slower than the conventional finite difference (FD) method. To<br />
further enhance the speed <strong>of</strong> the code, a red-black ordering is applied to solve the horizontal and vertical interface reduced<br />
systems. Numerical experiments on a 2-D MT problem <strong>of</strong> a given size running on a single processor machine shows that CPU<br />
time and memory used are almost constant for any resistivity models, frequencies and modes. This is a clear advantage <strong>of</strong><br />
our algorithm and is <strong>of</strong> particular importance if the method is applied to 3-D problems. We show that our new method<br />
results in reductions in both memory usage and CPU time for large enough domains when compared to the standard FD and<br />
HDD schemes. In addition, we also introduce a 'memory minimization map', a graphical tool we can use instead <strong>of</strong> trial-anderror<br />
to pre-select the optimal size <strong>of</strong> subdomains, which yield the best performance in both CPU time and memory even<br />
running on a serial machine. © <strong>2010</strong> The Authors Geophysical Journal <strong>International</strong> © <strong>2010</strong> RAS.<br />
No.1180<br />
Author (s) : Rungruang, T., Chaweeborisuit, P., Klosek, S.K.<br />
Title : Effect <strong>of</strong> malaria infection and dexamethasone on spleen morphology and histology<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 6, November <strong>2010</strong>, Pages 1290-1296<br />
Document Type : Article<br />
Keywords : Dexamethasone; Histology; Malaria; Spleen<br />
<strong>Abstract</strong> : The purpose <strong>of</strong> this study was to determine the histopathological changes <strong>of</strong> the spleen caused by parasite infection and<br />
steroid use to investigate pathological effects due to infection in ICR mice. The mice were divided into 5 groups: nonmalaria<br />
infected mice served as controls, mice with parasite infection only, and the other three groups; mice that were injected with<br />
dexamethasone (Dex) only, mice injected with Dex prior to and mice injected with Dex after malaria inoculation. Differences<br />
in spleen color between the groups were found. Compared to controls, malaria infected mice, and those injected with Dex<br />
only were significantly different (p < 0.05) in spleen weights and sizes. Histological changes were also seen in these two<br />
groups. Fused white pulps were found in the spleens <strong>of</strong> mice infected with malaria only, clear zones <strong>of</strong> white and red pulp<br />
were observed in the spleens <strong>of</strong> mice treated only with Dex; fibrinoids were also found in this group. The histology <strong>of</strong> spleens<br />
appeared normal except for infiltration by numerous megakaryocytes in the spleens <strong>of</strong> mice given Dex before or after<br />
parasite inoculation. Infection with malaria and use <strong>of</strong> Dex leads to destruction <strong>of</strong> typical features <strong>of</strong> spleen morphology and<br />
histology. However, uptake <strong>of</strong> Dex after malaria infection seems to reverse the pathology <strong>of</strong> the spleen.<br />
No.1181<br />
Author (s) : Ryan, D.P., Dias da Silva, M.R., Soong, T.W., Fontaine, B., Donaldson, M.R., Kung, A.W.C., Jongjaroenprasert, W., Liang, M.C.,<br />
Khoo, D.H.C., Cheah, J.S., Ho, S.C., Bernstein, H.S., Maciel, R.M.B., Brown Jr., R.H., Ptáček, L.J.<br />
Title : Mutations in Potassium Channel Kir2.6 Cause Susceptibility to Thyrotoxic Hypokalemic Periodic Paralysis<br />
Source : Cell, Volume 140, Issue 1, 8 January <strong>2010</strong>, Pages 88-98<br />
Document Type : Article<br />
Keywords : HUMDISEASE; PROTEINS; SIGNALING<br />
<strong>Abstract</strong> : Thyrotoxic hypokalemic periodic paralysis (TPP) is characterized by acute attacks <strong>of</strong> weakness, hypokalemia, and<br />
thyrotoxicosis <strong>of</strong> various etiologies. These transient attacks resemble those <strong>of</strong> patients with familial hypokalemic periodic<br />
paralysis (hypoKPP) and resolve with treatment <strong>of</strong> the underlying hyperthyroidism. Because <strong>of</strong> the phenotypic similarity <strong>of</strong><br />
these conditions, we hypothesized that TPP might also be a channelopathy. While sequencing candidate genes, we identified<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
a previously unreported gene (not present in human sequence databases) that encodes an inwardly rectifying potassium (Kir)<br />
channel, Kir2.6. This channel, nearly identical to Kir2.2, is expressed in skeletal muscle and is transcriptionally regulated by<br />
thyroid hormone. Expression <strong>of</strong> Kir2.6 in mammalian cells revealed normal Kir currents in whole-cell and single-channel<br />
recordings. Kir2.6 mutations were present in up to 33% <strong>of</strong> the unrelated TPP patients in our collection. Some <strong>of</strong> these<br />
mutations clearly alter a variety <strong>of</strong> Kir2.6 properties, all altering muscle membrane excitability leading to paralysis. © <strong>2010</strong><br />
Elsevier Inc. All rights reserved.<br />
No.1182<br />
Author (s) : Saechan, V., Nopparatana, C., Nopparatana, C., Fucharoen, S.<br />
Title : Molecular basis and hematological features <strong>of</strong> hemoglobin variants in Southern Thailand<br />
Source : <strong>International</strong> Journal <strong>of</strong> Hematology, Volume 92, Issue 3, October <strong>2010</strong>, Pages 445-450<br />
Document Type : Article<br />
Keywords : Abnormal hemoglobin; Hemoglobin variant; Molecular diagnosis; Thailand<br />
<strong>Abstract</strong> : Hemoglobinopathy (abnormal hemoglobin or hemoglobin variant) is an inherited disorder that results in the abnormal<br />
structure <strong>of</strong> globin chains <strong>of</strong> the hemoglobin (Hb) molecule. Many abnormal Hbs have been characterized worldwide,<br />
including more than 20 variants in Thailand. The Bio-Rad Variant II HPLC system is used for investigating hemoglobin variants<br />
at Songklanagarind Hospital. This system has been shown to be a sensitive, specific, and reproducible method, but some<br />
hemoglobin variants such as Hb Tak and Hb D-Punjab cannot, as yet, be clearly separated by this method. The aim <strong>of</strong> this<br />
study was to investigate the prevalence <strong>of</strong> hemoglobinopathy in southern Thailand using DNA sequencing and study the<br />
severity <strong>of</strong> each hemoglobin variant. A total <strong>of</strong> 58 hemoglobin variant samples were obtained from blood samples undergoing<br />
routine hemoglobin typing at Songklanagarind Hospital. Genomic DNAs were extracted from the samples, and the globin<br />
genes were analyzed by using PCR-direct sequencing. The molecular analysis revealed eight hemoglobin variants: 28 Hb C, 12<br />
Hb D-Punjab, 7 Hb Tak, 4 Hb G-Makassar, 2 Hb Lepore-Hollandia, 2 Hb Q-Thailand, 2 Hb O-Indonesia, and 1 Hb Hope. The<br />
distribution <strong>of</strong> hemoglobin variants in southern Thailand is associated with geographic and/or ethnic backgrounds. This study<br />
may help hematologists understand better the prevalence <strong>of</strong> hemoglobin variants and their hematological features in this<br />
region. © <strong>2010</strong> The Japanese Society <strong>of</strong> Hematology.<br />
No.1183<br />
Author (s) : Sae-Jie, W., Bunwong, K., Moore, E.J.<br />
Title : Qualitative behavior <strong>of</strong> SIS epidemic model on time scales<br />
Source : <strong>International</strong> Conference on Applied Mathematics, Simulation, Modelling - Proceedings, <strong>2010</strong>, Pages 159-164<br />
Document Type : Conference Paper<br />
Keywords : Bifurcation; Chaos; Limit cycles; Period doubling; SIS epidemic model; Time scales analysis<br />
<strong>Abstract</strong> : Mathematical models in continuous or discrete time are widely used to simplify real-world systems in order to understand<br />
their mechanisms for a particular purpose. Consequently, a welldefined model should be able to carry out some predictions<br />
and be fitted to observational data in a variety <strong>of</strong> time measurements (seconds, hours, days, weeks, months, or years).<br />
Therefore, the time scales approach also plays an important role in the model. In this paper, we construct a time scales<br />
version <strong>of</strong> a simple epidemic model (SIS) and explore the variety <strong>of</strong> its qualitative behavior. For each parameter value, the<br />
theory <strong>of</strong> time scales allows the discovery <strong>of</strong> similar and dissimilar behavior <strong>of</strong> SIS epidemic models on different time scales.<br />
Finally, the dynamic behavior shows a period doubling bifurcation path to chaos as the distance <strong>of</strong> equally spaced points in<br />
time increases.<br />
No.1184<br />
Author (s) : Sae-Jie, W., Bunwong, K., Moore, E.J.<br />
Title : The effect <strong>of</strong> time scales on SIS epidemic model<br />
Source : WSEAS Transactions on Mathematics, Volume 9, Issue 10, October <strong>2010</strong>, Pages 757-767<br />
Document Type : Article<br />
Keywords : Bifurcation; Chaos; Limit cycles; Period doubling; SIS epidemic model; Time scales calculus<br />
<strong>Abstract</strong> : The distribution <strong>of</strong> diseases is one <strong>of</strong> the most interesting real-world phenomena which can be systematically studied through<br />
a mathematical model. A well-known simple epidemic model with surprising dynamics is the SIS model. Usually, the time<br />
domains that are widely used in mathematical models are limited to real numbers for the case <strong>of</strong> continuous time or to<br />
integers for the case <strong>of</strong> discrete time. However, a disease pandemic such as an influenza pandemic regularly disappears from<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
a population and then recurs after a period <strong>of</strong> time. Additionally, collecting actual data continuously is time-consuming,<br />
relatively expensive, and really impractical. It seems that using a continuous-time model to describe observed data may not<br />
always be possible due to time domain conflict. The purpose <strong>of</strong> this paper is, therefore, to study the qualitative behavior <strong>of</strong><br />
SIS models on continuous, discrete, and mixed continuous-discrete time scales. We investigate their dynamic behavior and<br />
examine how this behavior changes in the different time scale domains. We show that the dynamic behavior can change in a<br />
systematic manner from simple stable steady-state solutions for the continuous time domain to complicated chaotic<br />
solutions for the discrete-time domain.<br />
No.1185<br />
Author (s) : Saelee, P., Wongkham, S., Chariyalertsak, S., Petmitr, S., Chuensumran, U.<br />
Title : RASSF1A promoter hypermethylation as a prognostic marker for hepatocellular carcinoma<br />
Source : Asian Pacific Journal <strong>of</strong> Cancer Prevention, Volume 11, Issue 6, <strong>2010</strong>, Pages 1677-1681<br />
Document Type : Article<br />
Keywords : DNA methylation; Hepatocellular carcinoma; Methylation-specific PCR; RASSF1A; Thailand<br />
<strong>Abstract</strong> : This study was performed to determine whether epigenetic aberrant methylation <strong>of</strong> RASSF1A might be associated with<br />
hepatocarcinogenesis. Methylation specific-PCR was performed to identify RASSF1A promoter hypermethylation in 29 tumors<br />
and corresponding normal liver tissues. In addition, RASSF1A mRNA levels were analyzed by quantitative real-time reverse<br />
transcription-PCR. Aberrant methylation <strong>of</strong> RASSF1A was detected in 25 <strong>of</strong> 29 cases (86%), with loss <strong>of</strong> RASSF1A expression<br />
evident in 8 <strong>of</strong> 22 cases (36%). No correlation between loss <strong>of</strong> RASSF1A mRNA and promoter hypermethylation <strong>of</strong> the<br />
RASSF1A gene was observed. There was a significant correlation between the methylation status <strong>of</strong> RASSF1A and<br />
hepatocellular carcinoma (HCC) patients who did not undergo chemotherapy (P = 0.03). Multivariate analysis, adjusted for<br />
tumor size, treatment, RASSF1A hypermethylation, and RASSF1A under-expression, showed RASSF1A hypermethylation to be<br />
assocaited with a better prognosis for HCC patients (HR= 0.089, 95%CI = 0.013-0.578; P = 0.012). Our findings showed that<br />
RASSF1A promoter hypermethylation occurs frequently, and may serve as a good prognostic factor.<br />
No.1186<br />
Author (s) : Sae-Oui, P., Sirisinha, C., Hatthapanit, K.<br />
Title : Properties <strong>of</strong> natural rubber filled with ultra fine acrylate rubber powder<br />
Source : Journal <strong>of</strong> Elastomers and Plastics, Volume 42, Issue 2, March <strong>2010</strong>, Pages 139-150<br />
Document Type : Article<br />
Keywords : acrylate rubber; natural rubber; rubber blend; rubber powder<br />
<strong>Abstract</strong> : Properties <strong>of</strong> natural rubber (NR) filled with various loadings <strong>of</strong> ultra-fine vulcanized acrylate rubber powder (ACMP) were<br />
investigated. ACMP loading was varied from 0 to 20 phr and, after compounding, the compound properties were determined.<br />
Results reveal that increasing ACMP loading leads to improved processability, as evidenced by the reduction <strong>of</strong> both mixing<br />
energy and Mooney viscosity. ACMP, however, has negative effect on cure, that is, both scorch time and optimum cure time<br />
are prolonged while the state <strong>of</strong> cure is reduced with increasing ACMP loading. Due to the reinforcing effect <strong>of</strong> the fine ACMP<br />
particles, both modulus and hardness are found to increase consecutively with increasing ACMP loading. The tensile strength<br />
is also found to improve with increasing ACMP loading up to 10 phr. However, due to the cure retardation effect and the high<br />
thermoplastic nature, the presence <strong>of</strong> ACMP causes deterioration <strong>of</strong> elasticity. As ACMP is highly polar and fully saturated, the<br />
addition <strong>of</strong> ACMP enhances the resistance to oil and thermal aging <strong>of</strong> the NR vulcanizate. Significant improvement <strong>of</strong> thermal<br />
aging resistance is found when 10 phr or more <strong>of</strong> ACMP is added. © The Author(s), <strong>2010</strong>.<br />
No.1187<br />
Author (s) : Sae-Oui, P., Sirisinha, C., Sa-nguanthammarong, P., Thaptong, P.<br />
Title : Properties and recyclability <strong>of</strong> thermoplastic elastomer prepared from natural rubber powder (NRP) and high density<br />
polyethylene (HDPE)<br />
Source : Polymer Testing, Volume 29, Issue 3, May <strong>2010</strong>, Pages 346-351<br />
Document Type : Article<br />
Keywords : High density polyethylene; Mechanical properties; Natural rubber powder; Recyclability; Thermoplastic natural rubber<br />
<strong>Abstract</strong> : Preparation <strong>of</strong> thermoplastic natural rubber (TPNR) was carried out by blending high density polyethylene (HDPE) with natural<br />
rubber powder (NRP) obtained from spray drying <strong>of</strong> pre-vulcanized natural rubber latex. The blend ratio <strong>of</strong> NRP/HDPE was<br />
varied and the properties and recyclability <strong>of</strong> the TPNRs were investigated. The results reveal that, due to flow restriction <strong>of</strong><br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
the crosslinked NRP, viscosity <strong>of</strong> the TPNR increases continuously with increasing NRP content. As expected, when NRP<br />
content is increased, properties <strong>of</strong> the TPNR are more rubber-like, as clearly observed from the stress-strain curve<br />
characteristics, the reductions in modulus and hardness and, most importantly, the significant improvement <strong>of</strong> permanent set.<br />
Although phase size <strong>of</strong> the dispersed NRP is relatively large, the tensile strength tends to increase gradually with increasing<br />
NRP content. The phenomenon <strong>of</strong> strain-induced crystallization is proposed to explain the results. It is also found that<br />
recycling has a noticeably detrimental influence on most properties <strong>of</strong> the TPNRs such as tensile strength, tear strength,<br />
hardness and tension set. This is thought to be a consequence <strong>of</strong> thermal degradation <strong>of</strong> the NRP during the recycling<br />
process. © <strong>2010</strong> Elsevier Ltd. All rights reserved.<br />
No.1188<br />
Author (s) : Sae-Oui, P., Sirisinha, C., Thaptong, P., Intiya, W.<br />
Title : Possible use <strong>of</strong> ultra-fine acrylonitrile butadiene rubber powder as filler in natural rubber vulcanizates<br />
Source : Journal <strong>of</strong> Applied Polymer Science, Volume 118, Issue 1, 5 October <strong>2010</strong>, Pages 353-358<br />
Document Type : Article<br />
Keywords : Aging; Blends; Fillers; Mechanical properties; Rubber<br />
<strong>Abstract</strong> : Possible use <strong>of</strong> ultra-fine acrylonitrile butadiene rubber powder (UFNBRP) as a filler for natural rubber (NR) was investigated.<br />
The UFNBRP was added into NR at various concentrations, and the compound properties were determined. It is found that,<br />
with increasing UFNBRP loading, the compound viscosity is increased, whereas both scorch time and optimum curing time are<br />
significantly reduced. The results also reveal that UFNBRP has negative effect not only on crosslink density but also on most<br />
mechanical properties <strong>of</strong> the vulcanizate, such as tensile strength, tear strength, compression set, and abrasion resistance.<br />
The deterioration <strong>of</strong> these mechanical properties is thought to arise mainly from the combined effect <strong>of</strong> large phase size <strong>of</strong><br />
the dispersed UFNBRP and low interfacial adhesion taking place from the polarity difference between UFNBRP and NR.<br />
Interestingly, it is found that, after aging, UFNBRP could promote postcuring phenomenon leading to increases <strong>of</strong> both relative<br />
100% modulus and relative tensile strength. Oil resistance is also found to improve considerably with increasing UFNBRP<br />
loading. This improvement is mainly attributed to the dilution effect, i.e., the higher the UFNBRP loading, the lower the NR<br />
portion and, thus, the greater the oil resistance <strong>of</strong> the vulcanizate. © <strong>2010</strong> Wiley Periodicals, Inc.<br />
No.1189<br />
Author (s) : Saepou, S., Pohmakotr, M., Reutrakul, V., Yoosook, C., Kasisit, J., Napaswad, C., Tuchinda, P.<br />
Title : Anti-HIV1 Diterpenoids from Leaves and Twigs <strong>of</strong> Polyalthia sclerophylla<br />
Source : Planta Medica, Volume 76, Issue 7, <strong>2010</strong>, Pages 721-725<br />
Document Type : Article<br />
Keywords : Annonaceae; AntiHIV1 activity; Ent kuarane diterpenoids; Polyalthia sclerophylla<br />
<strong>Abstract</strong> : Bioassay-guided fractionation and purification <strong>of</strong> the anti-HIV-1-active MeOH extract from the leaves and twigs <strong>of</strong> Polyalthia<br />
sclerophylla led to the isolation <strong>of</strong> two new compounds, ent-kaur-sclerodimer (1) and cyclotucanol 3-palmitate (2), along<br />
with the known ent-kaur-16-en-19-oic acid (3), 15-hydroxy-ent-kaur-16-en-19-oic acid (4), 15-acetoxy-ent-kaur-16-en-19-oic acid<br />
(5), 15-oxo-ent-kaur-16-en-19-oic acid (6), 16,17-dihydroxy-ent-kauran-19-oic acid (7), 16-hydroxy-ent-kauran-19-oic acid<br />
(xylopic acid) (8), a pseudodimer (15-hydroxy-ent-kaur-16-en-19-oic acid/17-hydroxy-ent-kaur-15-en-19-oic acid) (9), ermanin,<br />
nicotiflorin, and allantoin. Among these isolates, compound 3 was the most active in both anti-syncytium (EC50 13.7μg/mL<br />
and selectivity index 3.1) and HIV-1 reverse transcriptase (IC50 34.1μg/mL) assays. © Georg Thieme Verlag KG Stuttgart.<br />
No.1190<br />
Author (s) : Saetung, A., Rungvichaniwat, A., Campistron, I., Klinpituksa, P., Laguerre, A., Phinyocheep, P., Doutres, O., Pilard, J.-F.<br />
Title : Preparation and physico-mechanical, thermal and acoustic properties <strong>of</strong> flexible polyurethane foams basedon<br />
hydroxytelechelic natural rubber<br />
Source : Journal <strong>of</strong> Applied Polymer Science, Volume 117, Issue 2, 15 July <strong>2010</strong>, Pages 828-837<br />
Document Type : Article<br />
Keywords : Acoustic properties; Natural rubber; PHYSICO-mechanical properties; Polyurethane foams; Telechelic<br />
<strong>Abstract</strong> : Novel flexible polyurethane foams were successfully prepared from a renewable source, hydroxyte-lechelic natural rubber<br />
(HTNR) having different molecular weights (1000-3400 g mol-1) and variation <strong>of</strong> epoxide contents (EHTNR, 0-35% epoxidation)<br />
by a one-shot technique. The chemical and cell structures as well as physico-mechanical, thermal, and acoustic properties<br />
were characterized and compared with commercial polyol analogs. The obtained HTNR based foams are open cell structures<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
with cell dimensions between 0.38 and 0.47 mm. The HTNR1000 based foam exhibits better mechanical properties but lower<br />
elongation at break than those <strong>of</strong> commercial polyol analog. However, the HTNR3400 based foam shows the best elastic<br />
properties. In a series <strong>of</strong> EHTNR based foams, the tensile and compressive strengths show a tendency to increase with<br />
increasing epoxide content and amount <strong>of</strong> 1,4-butanediol (BD). The HTNR based foams demonstrate better low temperature<br />
flexibility than that <strong>of</strong> the foam based on commercial polyol. Moreover, the HTNR based polyurethane foams was found to<br />
be an excellent absorber <strong>of</strong> acoustics. © <strong>2010</strong> Wiley Periodicals, Inc.<br />
No.1191<br />
Author (s) : Saetung, A., Rungvichaniwat, A., Campistron, I., Klinpituksa, P., Laguerre, A., Phinyocheep, P., Pilard, J.-F.<br />
Title : Controlled degradation <strong>of</strong> natural rubber and modification <strong>of</strong> the obtained telechelic oligoisoprenes: Preliminary study <strong>of</strong><br />
their potentiality as polyurethane foam precursors<br />
Source : Journal <strong>of</strong> Applied Polymer Science, Volume 117, Issue 3, 1 August <strong>2010</strong>, Pages 1279-1289<br />
Document Type : Article<br />
Keywords : Controlled degradation; Natural rubber; Polyurethane foam oligoisoprenes; Telechelic<br />
<strong>Abstract</strong> : Telechelic oligoisoprenes were successfully prepared by the selective controlled degradation <strong>of</strong> natural rubber, a renewable<br />
source, via epoxidation and cleavage reactions. The molar mass <strong>of</strong> the oligoisoprene product obtained depends on the<br />
degree <strong>of</strong> epoxidation <strong>of</strong> the starting materials. The chemically modified structures obtained via epoxidation, hydrogenation,<br />
and ring opening <strong>of</strong> epoxide groups were also studied, and the chemical structures and thermal properties <strong>of</strong> the<br />
oligoisoprene products were determined. Moreover, the preliminary study <strong>of</strong> preparation <strong>of</strong> hydroxytelechelic natural rubber<br />
(HTNR)-based polyurethane foam was performed. A novel HTNR-based polyurethane foam was successfully prepared and its<br />
thermal properties were investigated and the results indicated that the HTNR-based polyurethane foam has a good low<br />
temperature flexibility. © <strong>2010</strong> Wiley Periodicals, Inc.<br />
No.1192<br />
Author (s) : Saetung, S., Chailurkit, L., Ongphiphadhanakul, B.<br />
Title : Acute changes in biochemical markers <strong>of</strong> bone resorption and formation after Thai traditional massage<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 7, July <strong>2010</strong>, Pages 771-775<br />
Document Type : Article<br />
Keywords : Bone formation; Bone resorption; Thai traditional massage<br />
<strong>Abstract</strong> : Background: Mechanical loadings by active exercise or passive low amplitude vibration have been demonstrated to enhance<br />
bone mass or delay bone loss. Traditional Thai massage can be anabolic to bone due to the application <strong>of</strong> physical loading<br />
on the body in a rhythmic fashion. Objective: To explore the skeletal effect <strong>of</strong> Thai traditional massage by examining the<br />
changes in biochemical markers <strong>of</strong> bone turnover immediately after the massage. Material and Method: Subjects consisted <strong>of</strong><br />
30 healthy females aged 20-40 years. Each subject received Thai traditional massage for 2 hours by a single masseuse. Bone<br />
mineral density (BMD) at baseline was measured by dual-energy X-ray absorptiometry (DEXA). C-terminal telopeptide <strong>of</strong> type I<br />
collagen (CTx-I) and total procollagen type 1 amino-terminal propeptide (P1NP) were determined by<br />
electrochemiluminescence immunoassay. Results: There was a 4.8% increase in serum P1NP concentrations after massage<br />
(median 43.4 ng/ml vs. 41.3 ng/ml, p < 0.05). Serum CTx-I also decreased after massage (median 2-hour vs. baseline 0.29<br />
ng/ml vs. 0.31 ng/ml, p < 0.05). There was a nearly significant negative correlation between the percentage change in serum<br />
P1NP and BMD at the total femur (r =-0.37, p = 0.056) whereas the statistically significant correlation disappeared between<br />
percentage change in bone turnover and the other sites <strong>of</strong> BMD. Conclusion: Thai traditional massage induces acute changes<br />
in bone formation and resorption markers. Study on the more prolonged effects <strong>of</strong> Thai traditional massage is warranted to<br />
explore its implication in the enhancement <strong>of</strong> bone health.<br />
No.1193<br />
Author (s) : Sae-Ung, K.; Govitrapong, P.; Ueda, K.; Phansuwan-Pujito, P.<br />
Title : MELATONIN DECREASES ALPHA-SYNUCLEIN IN MESOLIMBIC PATHWAY OF AMPHETAMINE-TREATED POSTNATAL RATS<br />
Source : JOURNAL OF NEUROCHEMISTRY, Volume: 115, Supplement: 1, Pages: 81-82, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
434
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No.1194<br />
Author (s) : Saewong, T., Ounjaijean, S., Mundee, Y., Pattanapanyasat, K., Fucharoen, S., Porter, J.B., Srichairatanakool, S.<br />
Title : Effects <strong>of</strong> green tea on iron accumulation and oxidative stress in livers <strong>of</strong> iron-challenged thalassemic mice<br />
Source : Medicinal Chemistry, Volume 6, Issue 2, <strong>2010</strong>, Pages 57-64<br />
Document Type : Article<br />
Keywords : Collagen; Green tea; Iron overload; Liver; Malondialdehyde; Thalassemia<br />
<strong>Abstract</strong> : Liver is affected by secondary iron overload in transfusions dependent β-thalassemia patients. The redox iron can generate<br />
reactive oxidants that damage biomolecules, leading to liver fibrosis and cirrhosis. Iron chelators are used to treat<br />
thalassemias to achieve negative iron balance and relieve oxidant-induced organ dysfunctions. Green tea (GT) (Camellia<br />
sinensis) catechins exhibit anti-oxidation, the inhibition <strong>of</strong> carcinogenesis, the detoxification <strong>of</strong> CYP2E1-catalyzed HepG2 cells<br />
and iron chelation. The purpose <strong>of</strong> this study was to investigate the effectiveness <strong>of</strong> GT in iron-challenged thalassemic mice.<br />
Heterozygous BKO type-thalassemia (BKO) mice (C57BL/6) experienced induced iron overload by being fed a ferrocenesupplemented<br />
diet (Fe diet) for 8 weeks, and by orally being given GT extract (300 mg/kg) and deferiprone (DFP) (50 mg/kg)<br />
for a further 8 weeks. Liver iron content (LIC) was analyzed by TPTZ colorimetric and Perl's staining techniques.<br />
Concentrations <strong>of</strong> liver reduced glutathione (GSH), collagen and malondialdehyde (MDA) were also measured. Dosages <strong>of</strong> the<br />
GT extract and DFP lowered LIC in the Fe diet-fed BKO mice effectively. The extract did not change any concentrations <strong>of</strong><br />
liver glutathione, collagen and MDA in the BKO mice. Histochemical examination showed leukocyte infiltration in the nearby<br />
hepatic portal vein and high iron accumulation in the livers <strong>of</strong> the iron-loaded BKO mice, however GT treatment lowered the<br />
elevated iron deposition. In conclusion, green tea inhibits or delays the deposition <strong>of</strong> hepatic iron in regularly iron-loaded<br />
thalassemic mice effectively. This will prevent the iron-induced generation <strong>of</strong> free radicals via Haber-Weiss and Fenton<br />
reactions, and consequently liver damage and fibrosis. Combined chelation with green tea would be investigated in βthalassemia<br />
patients with iron overload. © <strong>2010</strong> Bentham Science Publishers Ltd.<br />
No.1195<br />
Author (s) : Saglio, G., Kim, D.-W., Issaragrisil, S., Le Coutre, P., Etienne, G., Lobo, C., Pasquini, R., Clark, R.E., Hochhaus, A., Hughes, T.P.,<br />
Gallagher, N., Hoenekopp, A., Dong, M., Haque, A., Larson, R.A., Kantarjian, H.M.<br />
Title : Nilotinib versus imatinib for newly diagnosed chronic myeloid leukemia<br />
Source : New England Journal <strong>of</strong> Medicine, Volume 362, Issue 24, 17 June <strong>2010</strong>, Pages 2251-2259<br />
Document Type : Article<br />
Keywords : imatinib, nilotinib, 4 methyl N (3 (4 methylimidazol 1 yl) 5 (trifluoromethyl)phenyl) 3 ((4 pyridin 3 ylpyrimidin 2<br />
yl)amino)benzamide, 4-methyl-N-(3-(4-methylimidazol-1-yl)-5-(trifluoromethyl)phenyl)-3-((4-pyridin-3-ylpyrimidin-2yl)amino)benzamide,<br />
antineoplastic agent, BCR ABL protein, piperazine derivative, protein kinase inhibitor, pyrimidine<br />
derivative; adult, aged, alanine aminotransferase blood level, alkaline phosphatase blood<br />
level, alopecia, anemia, article, aspartate aminotransferase blood level, bilirubin blood level, blast cell crisis, cancer<br />
growth, chronic myeloid leukemia, clinical trial, controlled clinical trial, controlled study, creatinine blood<br />
level, cytogenetics, diarrhea, drug dose reduction, drug eruption, drug withdrawal, eyelid<br />
edema, fatigue, female, gastrointestinal disease, glucose blood level, headache, human, major clinical study, male, molecular<br />
mechanics, multicenter study, muscle spasm, myalgia, nausea, neutropenia, open study, periorbital edema, peripheral<br />
edema, Philadelphia chromosome positive cell, phosphate blood level, priority journal, pruritus, randomized controlled<br />
trial, side effect, skin disease, thrombocytopenia, treatment response, triacylglycerol lipase blood<br />
level, vomiting, adolescent, comparative study, disease course, drug antagonism, Kaplan Meier method, middle aged, myeloid<br />
leukemia, pathology, phase 3 clinical trial; Adolescent, Adult, Aged, Aged, 80 and over, Antineoplastic Agents, Blast<br />
Crisis, Disease Progression, Female, Fusion Proteins, bcr-abl, Humans, Kaplan-Meiers Estimate, Leukemia, Myeloid, Chronic-<br />
Phase, Male, Middle Aged, Piperazines, Protein Kinase Inhibitors, Pyrimidines, Young Adult<br />
<strong>Abstract</strong> : BACKGROUND: Nilotinib has been shown to be a more potent inhibitor <strong>of</strong> BCR-ABL than imatinib. We evaluated the efficacy<br />
and safety <strong>of</strong> nilotinib, as compared with imatinib, in patients with newly diagnosed Philadelphia chromosome-positive<br />
chronic myeloid leukemia (CML) in the chronic phase. METHODS: In this phase 3, randomized, open-label, multicenter study,<br />
we assigned 846 patients with chronic-phase Philadelphia chromosome-positive CML in a 1:1:1 ratio to receive nilotinib (at a<br />
dose <strong>of</strong> either 300 mg or 400 mg twice daily) or imatinib (at a dose <strong>of</strong> 400 mg once daily). The primary end point was the rate<br />
<strong>of</strong> major molecular response at 12 months. RESULTS: At 12 months, the rates <strong>of</strong> major molecular response for nilotinib (44%<br />
for the 300-mg dose and 43% for the 400-mg dose) were nearly twice that for imatinib (22%) (P
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and 78% for the 400-mg dose) than for imatinib (65%) (P
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No.1198<br />
Author (s) : Saiyood, S., Vangnai, A.S., Thiravetyan, P., Inthorn, D.<br />
Title : Bisphenol A removal by the Dracaena plant and the role <strong>of</strong> plant-associating bacteria<br />
Source : Journal <strong>of</strong> Hazardous Materials, Volume 178, Issue 1-3, June <strong>2010</strong>, Pages 777-785<br />
Document Type : Article<br />
Keywords : Bisphenol A; Dracaena plant; Plant-associating Bacillus sp; Plant-associating bacteria; Plant-associating Enterobacter sp.<br />
<strong>Abstract</strong> : Dracaena sanderiana and Dracaena fragrans plants, as representatives <strong>of</strong> native, tropical, evergreen plants with fibrous root<br />
systems, were evaluated for bisphenol A (BPA) tolerance and uptake capability. D. sanderiana demonstrated significantly<br />
higher BPA removal capability than D. fragrans. Therefore, it was chosen for further study. D. sanderiana tolerated BPA toxicity<br />
levels up to 80μM, while higher BPA concentrations damaged the plant. In the sterile hydroponic system with an initial BPA<br />
concentration <strong>of</strong> 20μM, the plant could uptake approximately 50% <strong>of</strong> the BPA. The plant's ability to translocate BPA was<br />
confirmed by the detection <strong>of</strong> BPA that accumulated at the roots and stems, but not at the leaves <strong>of</strong> the plant. Upon BPA<br />
exposure, the D. sanderiana secreted extracellular plant mucilage as a protective barrier to the toxic compound. In the nonsterile<br />
treatment, the BPA dissipation was contributed not only by the D. sanderiana plant, but also by the co-existing<br />
microbes. The BPA reached 85% <strong>of</strong> the initial concentration at 20μM. Among the six plant-associating bacterial isolates,<br />
Bacillus cereus strain BPW4 and Enterobacter sp. strain BPW5 colonized the D. sanderiana root surface and facilitated BPA<br />
dissipation in the hydroponic treatment system. In addition, the success <strong>of</strong> the BPA treatment in the hazardous waste landfill<br />
leachate demonstrated the potential application <strong>of</strong> D. sanderiana plant in the phytoremediation <strong>of</strong> BPA contaminated<br />
wastewater or industrial leachate. © <strong>2010</strong> Elsevier B.V.<br />
No.1199<br />
Author (s) : Sajan, C.P., Shahmoradi, B., Shivaraju, H.P., Rai, K.M.L., Ananda, S., Shayan, M.B., Thonthai, T., Rao, G.V.N., Byrappa, K.<br />
Title : Photocatalytic degradation <strong>of</strong> textile effluent using hydrothermally synthesised titania supported molybdenum oxide<br />
photocatalyst<br />
Source : Materials Research Innovations, Volume 14, Issue 1, 1 February <strong>2010</strong>, Pages 89-94<br />
Document Type : Conference Paper<br />
Keywords : Chemical oxygen demand; Hydrothermal; Photocatalytic; Textile effluent<br />
<strong>Abstract</strong> : The photocatalytic degradation <strong>of</strong> textile effluent using hydrothermally synthesised titania supported molybdenum oxide<br />
photocatalyst at 200°C with an autogenous pressure and experimental duration <strong>of</strong> 24 h has been reported. A control over the<br />
particle size, morphology and crystallinity <strong>of</strong> the photocatalyst has been studied with respect to the experimental parameters<br />
such as nutrient composition, solvent, pH, experimental duration, temperature and pressure. The products synthesised were<br />
characterised using XRD, SEM, FTIR, etc. The photodegradation <strong>of</strong> textile effluent using these composite was investigated<br />
under both solar and ultraviolet irradiation. The degradation <strong>of</strong> textile effluent was checked by the following parameters:<br />
chemical oxygen demand (COD), percentage transmission (%T), irradiation time and duration. The preliminary results are<br />
highly encouraging and further work is being carried out for the use <strong>of</strong> these photocatalytic compounds for other organic<br />
decomposition. © <strong>2010</strong> W. S. Maney & Son Ltd.<br />
No.1200<br />
Author (s) : Saksiri, B., Ruenwongsa, P.<br />
Title : Effects <strong>of</strong> a virtual sign animated pedagogical agent on ICT learning<br />
Source : <strong>International</strong> Journal <strong>of</strong> Learning, Volume 17, Issue 3, <strong>2010</strong>, Pages 37-50<br />
Document Type : Article<br />
Keywords : Deaf and hard <strong>of</strong> hearing; ICT learning; Sign language avatar<br />
<strong>Abstract</strong> : This study explored the use <strong>of</strong> a Virtual Sign Animated Pedagogical Agent (VSAPA) designed to assist Deaf and Hard <strong>of</strong> Hearing<br />
(DHH) college students' learning with a multimedia on Information and Computer Technology (ICT). VSAPA is a s<strong>of</strong>tware tool<br />
(avatar) designed to be the substitute for the sign language interpreter. Participants in this study were forty DHH college<br />
students randomly divided into four groups: one control group and three experimental groups. The first experimental group<br />
received ICT learning via the VSAPA learning environment. The second group was exposed to the same ICT content via a<br />
Hearing Teacher via Sign Language Interpreter (HTSLI). The third group studied by themselves using Text-Assisted (TA)<br />
instruction. The control group received none <strong>of</strong> the above treatment. Before the intervention, all groups were given the pretest<br />
on principles <strong>of</strong> computer and communication fundamental s<strong>of</strong>tware and hardware. One week later all groups received<br />
the post-test. The students in the three experimental groups were randomly selected for semi-structured interview. All the<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
teaching-learning processes were videotape-recorded and analyzed for students' response to the treatments. The results<br />
showed that students exposed to the VSAPA learning environment had more percentage gain in the post-test when<br />
compared those in the HTSLI and TA groups. The DHH students also had high positive attitudes toward the VSAPA learning<br />
environment. This study suggests that incorporating a virtual sign animated pedagogical agent into a multimedia learning<br />
environment can enhance DHH students' learning. © Common Ground, Benjaporn Saksiri, Pintip Ruenwongsa.<br />
No.1201<br />
Author (s) : Saksmerprome, V., Puiprom, O., Noonin, C., Flegel, T.W.<br />
Title : Detection <strong>of</strong> infectious hypodermal and haematopoietic necrosis virus (IHHNV) in farmed Australian Penaeus monodon by PCR<br />
analysis and DNA sequencing<br />
Source : Aquaculture, Volume 298, Issue 3-4, 7 January <strong>2010</strong>, Pages 190-193<br />
Document Type : Article<br />
Keywords : Australia; IHHNV; Infectious; Parvoviridae; PstDNV; Shrimp; Virus<br />
<strong>Abstract</strong> : Infectious hypodermal and haematopoietic necrosis virus (IHHNV) <strong>of</strong> shrimp has recently been classified as Penaeus stylirostris<br />
brevidensovirus (PstDNV) in the family Parvoviridae. Genetic variants have been reported in the Black Tiger shrimp Penaeus<br />
monodon over its natural range from East Africa to Australasia. Previous reports from Australia have suggested that a distinct,<br />
non-infectious type <strong>of</strong> IHHNV that is inserted into the shrimp host genome is the only type <strong>of</strong> IHHNV that occurs there. Here<br />
we describe an infectious type <strong>of</strong> IHHNV identified from farmed P. monodon in Australia in April 2008 by PCR amplification<br />
from two regions <strong>of</strong> the IHHNV genome, one reported to be discriminatory for the infectious type <strong>of</strong> IHHNV and the other<br />
(from IHHNV ORF1/2) not described in the non-infectious, inserted type. In addition, we used overlapping PCR primers to<br />
amplify all <strong>of</strong> the ORFs <strong>of</strong> the IHHNV genome (approximately 3.6 kb) from the Australian samples, except for the hairpin loop<br />
ends (GQ475529). Comparison <strong>of</strong> the maximum possible portion <strong>of</strong> this sequence with 8 GenBank records <strong>of</strong> IHHNV isolates<br />
reported from Asia (2814 bases relative to positions 588 to 3413 <strong>of</strong> GenBank AF273215) revealed 94-95% identity in nucleic<br />
acid sequence and 96 to 97% identity in amino acid sequence. These results were in agreement with an <strong>of</strong>ficial report by the<br />
World Organization for Animal Health in July 2008 for the presence <strong>of</strong> infectious IHHNV in Australia. © 2009 Elsevier B.V. All<br />
rights reserved.<br />
No.1202<br />
Author (s) : Salaemae, W., Junaid, M., Angsuthanasombat, C., Katzenmeier, G.<br />
Title : Structure-guided mutagenesis <strong>of</strong> active site residues in the dengue virus two-component protease NS2B-NS3<br />
Source : Journal <strong>of</strong> Biomedical Science, Volume 17, Issue 1, <strong>2010</strong>, Article number 68<br />
Document Type : Article<br />
Keywords : alanine, antivirus agent, mutant protein, nonstructural protein 2B, nonstructural protein 3, proteinase, recombinant<br />
protein, unclassified drug, virus protein, multiprotein complex, NS2B protein, flavivirus, NS3 protease, dengue virus, serine<br />
proteinase; amino acid sequence, amino acid substitution, article, catalysis, controlled study, Dengue virus, Dengue virus<br />
1, Dengue virus 2, Dengue virus 3, Dengue virus 4, enzyme active site, enzyme activity, enzyme assay, enzyme<br />
binding, enzyme conformation, enzyme kinetics, enzyme release, enzyme substrate, Escherichia coli, fluorescence<br />
analysis, fluorometry, gene mutation, nonhuman, priority journal, protein expression, protein purification, protein<br />
structure, protein targeting, site directed mutagenesis, structure activity relation, structure analysis, West Nile flavivirus, wild<br />
type, comparative study, enzyme specificity, enzymology, genetics, kinetics, methodology, protein conformation; Dengue<br />
virus; Catalytic Domain, Dengue Virus, Fluorometry, Kinetics, Multiprotein Complexes, Mutagenesis, Site-Directed, Protein<br />
Conformation, Serine Endopeptidases, Substrate Specificity, Viral Nonstructural Proteins<br />
<strong>Abstract</strong> : Background. The dengue virus two-component protease NS2B/NS3 mediates processing <strong>of</strong> the viral polyprotein precursor and<br />
is therefore an important determinant <strong>of</strong> virus replication. The enzyme is now intensively studied with a view to the<br />
structure-based development <strong>of</strong> antiviral inhibitors. Although 3-dimensional structures have now been elucidated for a<br />
number <strong>of</strong> flaviviral proteases, enzyme-substrate interactions are characterized only to a limited extend. The high selectivity<br />
<strong>of</strong> the dengue virus protease for the polyprotein precursor <strong>of</strong>fers the distinct advantage <strong>of</strong> designing inhibitors with exquisite<br />
specificity for the viral enzyme. To identify important determinants <strong>of</strong> substrate binding and catalysis in the active site <strong>of</strong> the<br />
dengue virus NS3 protease, nine residues, L115, D129, G133, T134, Y150, G151, N152, S163 and I165, located within the S1<br />
and S2 pockets <strong>of</strong> the enzyme were targeted by alanine substitution mutagenesis and effects on enzyme activity were<br />
fluorometrically assayed. Methods. Alanine substitutions were introduced by site-directed mutagenesis at residues L115, D129,<br />
G133, T134, Y150, G151, N152, S163 and I165 and recombinant proteins were purified from overexpressing E. coli. Effects <strong>of</strong><br />
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these substitutions on enzymatic activity <strong>of</strong> the NS3 protease were assayed by fluorescence release from the synthetic model<br />
substrate GRR-amc and kinetic parameters Km, kcatand kcat/K mwere determined. Results. Kinetic data for mutant derivatives<br />
in the active site <strong>of</strong> the dengue virus NS3 protease were essentially in agreement with a functional role <strong>of</strong> the selected<br />
residues for substrate binding and/or catalysis. Only the L115A mutant displayed activity comparable to the wild-type<br />
enzyme, whereas mutation <strong>of</strong> residues Y150 and G151 to alanine completely abrogated enzyme activity. A G133A mutant had<br />
an approximately 10-fold reduced catalytic efficiency thus suggesting a critical role for this residue seemingly as part <strong>of</strong> the<br />
oxyanion binding hole. Conclusions. Kinetic data obtained for mutants in the NS3 protease have confirmed predictions for the<br />
conformation <strong>of</strong> the active site S1 and S2 pockets based on earlier observations. The data presented herein will be useful to<br />
further explore structure-activity relationships <strong>of</strong> the flaviviral proteases important for the structure-guided design <strong>of</strong> novel<br />
antiviral therapeutics. © <strong>2010</strong> Salaemae et al; licensee BioMed Central Ltd.<br />
No.1203<br />
Author (s) : Saleesung, T., Saeoui, P., Sirisinha, C.<br />
Title : Mechanical and thermal properties <strong>of</strong> thermoplastic elastomer based on low density polyethylene and ultra-fine fullyvulcanized<br />
acrylonitrile butadiene rubber powder (UFNBRP)<br />
Source : Polymer Testing, Volume 29, Issue 8, December <strong>2010</strong>, Pages 977-983<br />
Document Type : Article<br />
Keywords : Low density polyethylene; Nitrile rubber; Rubber powder; Thermoplastic elastomer<br />
<strong>Abstract</strong> : Ultra-fine fully vulcanized acrylonitrile butadiene rubber powder (UFNBRP) and low density polyethylene (LDPE) were used<br />
for preparing thermoplastic vulcanizates (TPVs). A simple blending technique was utilized as an alternative to a complex<br />
dynamic vulcanization process typically used for the preparation <strong>of</strong> TPVs. Blend composition ratio <strong>of</strong> UFNBRP/LDPE was<br />
varied, and the properties <strong>of</strong> the TPVs were investigated. Morphological result reveals that all blends exhibit a two-phase<br />
morphology in which the UFNBRP particles form the dispersed phase in the LDPE matrix. This implies successful TPV<br />
preparation. Viscoelastic behavior <strong>of</strong> the TPVs was found to be governed by UFNBRP loading. As expected, with increasing<br />
UFNBRP loading, the elastic contribution increases as evidenced by the decreases in sensitivity <strong>of</strong> storage modulus to test<br />
frequency and in creep compliance. The rheological results are in line with the improvements in rebound resilience and<br />
compression set. The presence <strong>of</strong> chemical crosslinks in UFNBRP in conjunction with the formation <strong>of</strong> a transient network <strong>of</strong><br />
rubber particles is thought to be responsible for such increase in elastic response. Additionally, degree <strong>of</strong> crystallinity and oil<br />
resistance <strong>of</strong> the TPVs were found to decrease with an increase in UFNBRP loading. The suppression <strong>of</strong> LDPE crystallization is<br />
probably caused by the UFNBRP particles locating in the interlamellar spaces <strong>of</strong> the LDPE matrix. © <strong>2010</strong> Elsevier Ltd. All<br />
rights reserved.<br />
No.1204<br />
Author (s) : Saletta, F., Rahmanto, Y.S., Noulsri, E., Richardson, D.R.<br />
Title : Iron chelator-mediated alterations in gene expression: Identification <strong>of</strong> novel iron-regulated molecules that are molecular<br />
targets <strong>of</strong> hypoxia-inducible factor-1α and p53<br />
Source : Molecular Pharmacology, Volume 77, Issue 3, March <strong>2010</strong>, Pages 443-458<br />
Document Type : Article<br />
Keywords : 2 hydroxy 1 naphthylaldehyde isonicotinoyl hydrazone, amyloid precursor protein, chelating agent, complementary<br />
DNA, complementary RNA, deferoxamine, early growth response factor 1, growth differentiation factor 15, hydrazone<br />
derivative, hypoxia inducible factor 1alpha, iron, ligand, messenger RNA, protein p53, transcription factor, unclassified drug;<br />
apoptosis, article, cancer inhibition, cell strain MCF 7, chemical structure, controlled study, gene expression<br />
regulation, genome, human, human cell, iron chelation, iron deficiency, microarray analysis, nucleotide sequence, priority<br />
journal, reverse transcription polymerase chain reaction, tumor cell, Western blotting; Animals, Apoptosis, Cell<br />
Proliferation, Cells, Cultured, Gene Expression Regulation, Gene Targeting, Genes, p53, Growth Inhibitors, Humans, Hypoxia-<br />
Inducible Factor 1, alpha Subunit, Iron, Iron Chelating Agents, Iron-Regulatory Proteins, Mice, Mice, Knockout, Tumor Cells,<br />
Cultured<br />
<strong>Abstract</strong> : Iron deficiency affects 500 million people, yet the molecular role <strong>of</strong> iron in gene expression remains poorly characterized. In<br />
addition, the alterations in global gene expression after iron chelation remain unclear and are important to assess for<br />
understanding the molecular pathology <strong>of</strong> iron deficiency and the biological effects <strong>of</strong> chelators. Considering this, we<br />
assessed the effect on whole genome gene expression <strong>of</strong> two iron chelators (desferrioxamine and 2-hydroxy-1napthylaldehyde<br />
isonicotinoyl hydrazone) that have markedly different permeability properties. Sixteen genes were<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
significantly regulated by both ligands, whereas a further 50 genes were significantly regulated by either compound. Apart<br />
from ironmediated regulation <strong>of</strong> expression via hypoxia inducible factor-1α, it was noteworthy that the transcription factor<br />
p53 was also involved in iron-regulated gene expression. Examining 16 genes regulated by both chelators in normal and<br />
neoplastic cells, five genes (APP, GDF15, CITED2, EGR1, and PNRC1) were significantly differentially expressed between the cell<br />
types. In view <strong>of</strong> their functions in tumor suppression, proliferation, and apoptosis, these findings are important for<br />
understanding the selective antiproliferative effects <strong>of</strong> chelators against neoplastic cells. Most <strong>of</strong> the genes identified have<br />
not been described previously to be iron-regulated and are important for understanding the molecular and cellular effects <strong>of</strong><br />
iron depletion. Copyright © <strong>2010</strong> The American Society for Pharmacology and Experimental Therapeutics.<br />
No.1205<br />
Author (s) : Sangjan, S., Traiphol, N., Traiphol, R.<br />
Title : Improvement <strong>of</strong> ultrathin polystyrene film stability by addition <strong>of</strong> poly(styrene-stat-chloromethylstyrene) copolymer: An<br />
atomic force microscopy study<br />
Source : Thin Solid Films, Volume 518, Issue 17, 30 June <strong>2010</strong>, Pages 4879-4883<br />
Document Type : Article<br />
Keywords : Additive; Atomic force microscopy; Dewetting; Interface structure; Polystyrene; Thin films<br />
<strong>Abstract</strong> : A method to improve the stability <strong>of</strong> ultrathin polystyrene (PS) films on SiOx/Si substrate is introduced. In this method,<br />
interfacial interactions between PS film and substrate are enhanced by addition <strong>of</strong> poly(styrene-statchloromethylstyrene(ClMS))<br />
copolymer containing 5 mol% <strong>of</strong> ClMS group. The resulting slight structural modification <strong>of</strong> the<br />
copolymer does not cause phase separation in the polymer blend. On the other hand, the existence <strong>of</strong> polar ClMS groups<br />
provides anchoring sites on the polar SiOx surface via dipolar interactions. In this study, ratios <strong>of</strong> the copolymers are varied<br />
from 0 to 40 wt.% in the thin films resulting in a systematic increase <strong>of</strong> the interfacial interactions. The dewetting behaviors <strong>of</strong><br />
all films subjected to the same annealing conditions are explored via atomic force microscopy. The analyses <strong>of</strong> root mean<br />
square roughness and dewetting area as a function <strong>of</strong> annealing time and copolymer ratio provide information about the film<br />
stability. Our results indicate that blending small quantity <strong>of</strong> the copolymer with PS significantly increases the stability <strong>of</strong><br />
ultrathin films. © <strong>2010</strong> Elsevier B.V. All rights reserved.<br />
No.1206<br />
Author (s) : Sangket, U., Mahasirimongkol, S., Chantratita, W., Tandayya, P., Aulchenko, Y.S.<br />
Title : ParallABEL: An R library for generalized parallelization <strong>of</strong> genome-wide association studies<br />
Source : BMC Bioinformatics, Volume 11, 29 April <strong>2010</strong>, Article number 217<br />
Document Type : Article<br />
Keywords : article, computer program, gene library, genetic association, genome, genomics, methodology, single nucleotide<br />
polymorphism; Gene Library, Genome, Genome-Wide Association Study, Genomics, Polymorphism, Single<br />
Nucleotide, S<strong>of</strong>tware<br />
<strong>Abstract</strong> : Background: Genome-Wide Association (GWA) analysis is a powerful method for identifying loci associated with complex traits<br />
and drug response. Parts <strong>of</strong> GWA analyses, especially those involving thousands <strong>of</strong> individuals and consuming hours to<br />
months, will benefit from parallel computation. It is arduous acquiring the necessary programming skills to correctly partition<br />
and distribute data, control and monitor tasks on clustered computers, and merge output files.Results: Most components <strong>of</strong><br />
GWA analysis can be divided into four groups based on the types <strong>of</strong> input data and statistical outputs. The first group<br />
contains statistics computed for a particular Single Nucleotide Polymorphism (SNP), or trait, such as SNP characterization<br />
statistics or association test statistics. The input data <strong>of</strong> this group includes the SNPs/traits. The second group concerns<br />
statistics characterizing an individual in a study, for example, the summary statistics <strong>of</strong> genotype quality for each sample. The<br />
input data <strong>of</strong> this group includes individuals. The third group consists <strong>of</strong> pair-wise statistics derived from analyses between<br />
each pair <strong>of</strong> individuals in the study, for example genome-wide identity-by-state or genomic kinship analyses. The input data<br />
<strong>of</strong> this group includes pairs <strong>of</strong> SNPs/traits. The final group concerns pair-wise statistics derived for pairs <strong>of</strong> SNPs, such as the<br />
linkage disequilibrium characterisation. The input data <strong>of</strong> this group includes pairs <strong>of</strong> individuals. We developed the<br />
ParallABEL library, which utilizes the Rmpi library, to parallelize these four types <strong>of</strong> computations. ParallABEL library is not<br />
only aimed at GenABEL, but may also be employed to parallelize various GWA packages in R. The data set from the North<br />
American Rheumatoid Arthritis Consortium (NARAC) includes 2,062 individuals with 545,080, SNPs' genotyping, was used to<br />
measure ParallABEL performance. Almost perfect speed-up was achieved for many types <strong>of</strong> analyses. For example, the<br />
computing time for the identity-by-state matrix was linearly reduced from approximately eight hours to one hour when<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
ParallABEL employed eight processors.Conclusions: Executing genome-wide association analysis using the ParallABEL library<br />
on a computer cluster is an effective way to boost performance, and simplify the parallelization <strong>of</strong> GWA studies. ParallABEL is<br />
a user-friendly parallelization <strong>of</strong> GenABEL. © <strong>2010</strong> Sangket et al; licensee BioMed Central Ltd.<br />
No.1207<br />
Author (s) : Sangruchi, T.; Phukaoloun, M.; Manonukul, J.; Sithinamsuwan, P.<br />
Title : Pathology <strong>of</strong> pythium arteritis<br />
Source : HISTOPATHOLOGY, Volume: 57, Supplement: 1, Pages: 176-176, Meeting <strong>Abstract</strong>: 491, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1208<br />
Author (s) : Sangsupawanich, P., Mahakittikun, V., Chongsuvivatwong, V., Mo-suwan, L., Choprapawon, C.<br />
Title : Effect <strong>of</strong> helminthic infections together with mite allergen exposure on the risk <strong>of</strong> wheeze in preschool children<br />
Source : Asian Pacific Journal <strong>of</strong> Allergy and Immunology, Volume 28, Issue 1, March <strong>2010</strong>, Pages 29-34<br />
Document Type : Article<br />
Keywords : house dust allergen; article, Ascaris lumbricoides, child, cohort analysis, controlled study, disease association, disease<br />
predisposition, dust exposure, environmental exposure, feces analysis, female, helminthiasis, hookworm, hookworm<br />
infection, human, information processing, informed consent, major clinical study, male, parasite transmission, population<br />
based case control study, preschool child, prevalence, prospective study, questionnaire, Thailand, Trichuris, wheezing;<br />
Animals, Antigens, Dermatophagoides, Ascariasis, Ascaris lumbricoides, Child,<br />
Preschool, Female, Hospitalization, Humans, Hypersensitivity, Infant, Male, Prospective<br />
Studies, Pyroglyphidae, Questionnaires, Respiratory Sounds, Risk Factors, Skin Tests, Thailand<br />
<strong>Abstract</strong> : Although some studies have indicated that helminthic infections and house dust mite exposure may have an alleviating<br />
effect on wheeze, an interaction effect <strong>of</strong> both risk factors has not been examined in Thailand. The objective <strong>of</strong> this study<br />
was to investigate whether exposure to helminthic infections together with house dust mite allergen was associated with<br />
wheeze in children aged 18-36 months, living in an area <strong>of</strong> southern Thailand where helminthic infections are endemic. This<br />
study was undertaken as a part <strong>of</strong> The Prospective Cohort Study <strong>of</strong> Thai Children which recruited 1,076 children born<br />
between December 2000 and November 2001. A home dust sample was collected once when the infants were 12-18 months<br />
old to measure house dust mite allergen (Der p1). Questionnaire data on wheeze and a stool specimen for soil-transmitted<br />
helminth analysis were collected at age 18-36 months. Prevalence <strong>of</strong> exposure to house dust mite allergen (Der p1) > 10<br />
μg/g dust was 31.8%. Soiltransmitted helminths were present in 25.0%, predominately Ascaris lumbricoides. Hookworm<br />
infection was associated with a physician's diagnosis <strong>of</strong> wheeze (OR 4.20, 95% CI 1.45-12.10) and hospitalized wheeze (OR<br />
5.40, 95% CI 1.26-23.01). Hospitalized wheeze was significantly higher in subjects exposed to Der p1 2-10 μg/g dust.<br />
Helminthic infections were not associated with a significant interaction effect with mite allergen against a risk <strong>of</strong> wheeze. Our<br />
survey confirms that hookworm infection and mite allergen exposure are independent risk factors for childhood wheeze and<br />
there is no evidence <strong>of</strong> important interaction between the two.<br />
No.1209<br />
Author (s) : Sangsuriya, P., Rojtinnakorn, J., Senapin, S., Flegel, T.W.<br />
Title : Identification and characterization <strong>of</strong> Alix/AIP1 interacting proteins from the black tiger shrimp, Penaeus monodon<br />
Source : Journal <strong>of</strong> Fish Diseases, Volume 33, Issue 7, July <strong>2010</strong>, Pages 571-581<br />
Document Type : Article<br />
Keywords : Alix; Apoptosis; Guanylyl cyclase; Penaeus monodon; Ubiquitin; White spot syndrome virus<br />
<strong>Abstract</strong> : Apoptosis is proposed to be a major cause <strong>of</strong> death in shrimp viral infections. From our previous study, an apoptosis-related<br />
gene, Pm-Alix, was identified from the black tiger shrimp. Its expression was high in defence-related tissues including<br />
haemocytes and the lymphoid organ. To clarify its possible role in shrimp, we used Pm-Alix as bait in a yeast two-hybrid<br />
analysis to search for Alix interacting proteins in shrimp. Two cDNA sequences discovered had homology to a predicted<br />
ubiquitin C <strong>of</strong> the purple sea urchin, Strongylocentrotus purpuratus, and to a guanylyl cyclase <strong>of</strong> the red swamp crayfish,<br />
Procambarus clarkii. In vitro pull-down assays confirmed positive interaction between Pm-Alix and both proteins. Tissue<br />
distribution analysis revealed that Pm-Alix and the two binding partners were widely expressed in various tissues but more<br />
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highly expressed in haemocytes. However, no significant positive or negative correlation was found in the expression <strong>of</strong> these<br />
genes as shrimp approached morbidity and death after challenge with white spot syndrome virus. Thus, the results suggested<br />
that Alix and its interacting partners did not play a direct role related to shrimp death. © <strong>2010</strong> Blackwell Publishing Ltd.<br />
No.1210<br />
Author (s) : Sa-nguanmoo, P.; Thawornsuk, N.; Rianthavorn, P.; Sommanustweechai, A.; Ratanakorn, P.; Poovorawan, Y.<br />
Title : High prevalence <strong>of</strong> antibodies against hepatitis A virus among captive nonhuman primates<br />
Source : PRIMATES, Volume: 51, Issue: 2, Pages: 167-170, DOI: 10.1007/s10329-009-0172-z, Published: APR <strong>2010</strong><br />
Document Type : Article<br />
Keywords : Hepatitis A virus; Captive nonhuman primates; EIA; HAV antibodies<br />
<strong>Abstract</strong> : Hepatitis A virus (HAV) can infect not only humans but also several other nonhuman primates. This study has been<br />
conducted to evaluate the comprehensive anti-HAV seroprevalence in captive nonhuman primate populations in Thailand.<br />
The prevalence <strong>of</strong> antibodies against HAV in 96 captive nonhuman primates <strong>of</strong> 11 species was evaluated by competitive<br />
enzyme immunoassay (EIA). HAV antibodies were found in 64.7% (11/17) <strong>of</strong> macaques, 85.7% (6/7) <strong>of</strong> langurs, 28.4% (10/35)<br />
<strong>of</strong> gibbons, and 94.6% (35/37) <strong>of</strong> orangutans. However, anti-HAV IgM was not found in any sera. These results indicate that the<br />
majority <strong>of</strong> captive nonhuman primates in Thailand were exposed to HAV. It is possible that some <strong>of</strong> the animals were<br />
infected prior to capture.<br />
No.1211<br />
Author (s) : Sansukcharearnpon, A., Wanichwecharungruang, S., Leepipatpaiboon, N., Kerdcharoen, T., Arayachukeat, S.<br />
Title : High loading fragrance encapsulation based on a polymer-blend: Preparation and release behavior<br />
Source : <strong>International</strong> Journal <strong>of</strong> Pharmaceutics, Volume 391, Issue 1-2, May <strong>2010</strong>, Pages 267-273<br />
Document Type : Article<br />
Keywords : Controlled release; Encapsulation; Essential oil; Fragrance; Nanoparticles; TGA<br />
<strong>Abstract</strong> : The six fragrances, camphor, citronellal, eucalyptol, limonene, menthol and 4-tert-butylcyclohexyl acetate, which represent<br />
different chemical functionalities, were encapsulated with a polymer-blend <strong>of</strong> ethylcellulose (EC), hydroxypropyl<br />
methylcellulose (HPMC) and poly(vinyl alcohol) (PV(OH)) using solvent displacement (ethanol displaced by water). The<br />
process gave ≥40% fragrance loading capacity with ≥80% encapsulation efficiency at the fragrance to polymer weight ratio <strong>of</strong><br />
1:1 and at initial polymer concentrations <strong>of</strong> 2000-16,000. ppm and the obtained fragrance-encapsulated spheres showed<br />
hydrodynamic diameters <strong>of</strong> less than 450. nm. The release pr<strong>of</strong>ile <strong>of</strong> the encapsulated fragrances, evaluated by both thermal<br />
gravimetric and electronic nose techniques, indicated different release characteristics amongst the six encapsulated<br />
fragrances. Limonene showed the fastest release with essentially no retention by the nanoparticles, while eucalyptol and<br />
menthol showed the slowest release. © <strong>2010</strong> Elsevier B.V.<br />
No.1212<br />
Author (s) : Santawisuk, W., Kanchanavasita, W., Sirisinha, C., Harnirattisai, C.<br />
Title : Dynamic viscoelastic properties <strong>of</strong> experimental silicone s<strong>of</strong>t lining materials<br />
Source : Dental Materials Journal, Volume 29, Issue 4, <strong>2010</strong>, Pages 454-460<br />
Document Type : Article<br />
Keywords : Dynamic viscoelastic properties; Silicone elastomer; S<strong>of</strong>t lining material<br />
<strong>Abstract</strong> : The purpose <strong>of</strong> this study was to evaluate the dynamic viscoelastic properties <strong>of</strong> experimental silicone s<strong>of</strong>t lining materials,<br />
Silastic® 4-4210 reinforced with silica fillers. Storage modulus (E'), loss modulus (E") and damping factor (tan δ) were<br />
determined using mechanical analyzer under a deformation strain level <strong>of</strong> 0.27% at test frequency and a temperature range<br />
<strong>of</strong> 1 Hz and 0 to respectively. The degree <strong>of</strong> silica dispersion was also studied using a field emission scanning electron<br />
microscopy (FE-SEM). ANOVA and Tukey's HSD test results indicated that the prepared silicone elastomers provided a<br />
significantly greater factor, but less storage modulus than GC Reline S<strong>of</strong>t and Tokuyama S<strong>of</strong>reliner Tough (p
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Effectiveness <strong>of</strong> pharmacist-participated warfarin therapy management: A systematic review and meta-analysis<br />
Source : Journal <strong>of</strong> Thrombosis and Haemostasis, Volume 8, Issue 11, November <strong>2010</strong>, Pages 2418-2427<br />
Document Type : Article<br />
Keywords : Anticoagulation clinic; Bleeding; Meta-analysis; Pharmacist; Systematic review; Thromboembolism; Warfarin<br />
<strong>Abstract</strong> : Objective: Although pharmacist-participated warfarin therapy management (PWTM) has been accepted and implemented in<br />
various parts <strong>of</strong> the world, the evidence demonstrating the effects <strong>of</strong> PWTM compared with usual care on clinical outcomes<br />
is lacking. We performed a systematic review and meta-analysis to compare the effects <strong>of</strong> PWTM with usual care on bleeding<br />
and thromboembolic outcomes. Methods: We searched MEDLINE, SCOPUS, EMBASE, IPA, CINAHL, Cochrane CENTRAL, Thai<br />
Index Medicus and Thai Medical Index, and reference lists <strong>of</strong> studies, without language restriction. Databases were searched<br />
from their inception to July 2009. The studies using warfarin as an anticoagulant with sufficient data for compilation <strong>of</strong> 2 × 2<br />
tables were included. Both randomized controlled trials (RCTs) and non-RCTs were considered. Two authors independently<br />
reviewed each study, assigned quality scores and extracted data for all outcomes using a standardized form. Pooled effect<br />
estimates (risk ratio; RR) were obtained using a random effects model. Result: Of 661 articles identified, 24 studies with<br />
728,377 patients were included. In the random-effects meta-analysis <strong>of</strong> RCTs, the PWTM group had statistically significant<br />
effects on the prevention <strong>of</strong> total bleeding [RR, 0.51; 95% confidence interval (CI), 0.28-0.94]. However, the effects on major<br />
bleeding (RR, 0.64; 95% CI, 0.18-2.36), thromboembolic events (RR, 0.79; 95% CI, 0.33-1.93), all-cause mortality (RR, 0.93; 95%<br />
CI, 0.41-2.13) and warfarin-related mortality (RR, 0.65; 95% CI, 0.18-2.42) were not significant. Conclusion: Pharmacist's<br />
participation in the management <strong>of</strong> warfarin therapy significantly reduces total bleeding, with a non-significant trend towards<br />
decreases in other warfarin-related complications. © <strong>2010</strong> <strong>International</strong> Society on Thrombosis and Haemostasis.<br />
No.1214<br />
Author (s) : Saowakon, N.; Chaichanasak, P.; Wanichanon, C.; Reutrakul, V; Sobhon, P.<br />
Title : In vitro effect <strong>of</strong> an aqueous extract <strong>of</strong> Artocarpus lakoocha on the intestinal parasites in cattle<br />
Source : PLANTA MEDICA, Volume: 76, Issue: 12, Pages: 1298-1298, Published: AUG <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1215<br />
Author (s) : Sappat, A., Jaroenram, W., Kiatpathomchai, W., Lomas, T., Tuantranont, A.<br />
Title : Turbidity detection <strong>of</strong> shrimp taura syndrome virus by loop-mediated isothermal amplification reaction<br />
Source : INEC <strong>2010</strong> - <strong>2010</strong> 3rd <strong>International</strong> Nanoelectronics Conference, Proceedings, <strong>2010</strong>, Article number 5424675, Pages 265-266<br />
Document Type : Conference Paper<br />
Keywords : Agarose gel electrophoresis, Detection limits, Disease diagnosis, Economic loss, Gene amplification, Novel methods, Optimal<br />
temperature, Reverse transcription, Sample solution, Shrimp aquaculture, Spectroscopic measurements, Thailand, Turbidity<br />
measurements; Agriculture, Aquaculture, Computer viruses, Detectors, Diagnosis, Electrophoresis, Gels, Light emitting<br />
diodes, Magnesium, Nanoelectronics, Nucleic acids, Physical optics, Synthesis (chemical), Transcription, Turbidity, Viruses;<br />
Amplification<br />
<strong>Abstract</strong> : Reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay is a novel method <strong>of</strong> gene amplification that<br />
amplifies nucleic acid, which can be applied for disease diagnosis in shrimp aquaculture. During the LAMP reaction, the white<br />
precipitate <strong>of</strong> magnesium pyrophosphate (Mg2P 2O7) is formed correlates with the amount <strong>of</strong> synthesized DNA. So, the<br />
turbidity can be measured. In this study, a portable turbidimeter has been developed for field to detection <strong>of</strong> Taura<br />
Syndrome Virus (TSV) that causes large economic losses to most major shrimp-producing countries including Thailand. The<br />
device could maintain an optimal temperature (63 °C) for 25 μl <strong>of</strong> LAMP sample solution contained in a 0.2 ml commercial<br />
PCR tube. We also applied the spectroscopic measurement technique to monitor a by-product <strong>of</strong> LAMP reaction, light<br />
emitting diode (LED) was used as a light source. Light dependent resistance (LDR) was used as detector. The results obtained<br />
from turbidity measurement revealed the same detection limit to those from agarose gel electrophoresis method. ©<strong>2010</strong><br />
IEEE.<br />
No.1216<br />
Author (s) : Sarapusit, S., Pethuan, S., Rongnoparut, P.<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Mosquito NADPH-cytochrome P450 oxidoreductase: Kinetics and role <strong>of</strong> phenylalanine amino acid substitutions at leu86 and<br />
leu219 in CYP6AA3-mediated deltamethrin metabolism<br />
Source : Archives <strong>of</strong> Insect Biochemistry and Physiology, Volume 73, Issue 4, April <strong>2010</strong>, Pages 232-244<br />
Document Type : Article<br />
Keywords : Anopheles minimus; CYP6AA3; CYPOR; Deltamethrin; Flavin c<strong>of</strong>actors; Kinetic study<br />
<strong>Abstract</strong> : The NADPH-cytochrome P450 oxidoreductase (CYPOR) enzyme is a membrane-bound protein and contains both FAD and<br />
FMN c<strong>of</strong>actors. The enzyme transfers two electrons, one at a time, from NADPH to cytochrome P450 enzymes to function in<br />
the enzymatic reactions. We previously expressed in Escherichia coli the membrane-bound CYPOR (flAnCYPOR) from<br />
Anopheles minimus mosquito. We demonstrated the ability <strong>of</strong> flAnCYPOR to support the An. minimus CYP6AA3 enzyme<br />
activity in deltamethrin degradation in vitro. The present study revealed that the flAnCYPOR purified enzyme, analyzed by a<br />
fluorometric method, readily lost its flavin c<strong>of</strong>actors. When supplemented with exogenous flavin c<strong>of</strong>actors, the activity <strong>of</strong><br />
flAnCYPOR-mediated cytochrome c reduction was increased. Mutant enzymes containing phenylalanine substitutions at<br />
leucine residues 86 and 219 were constructed and found to increase retention <strong>of</strong> FMN c<strong>of</strong>actor in the flAnCYPOR enzymes.<br />
Kinetic study by measuring cytochrome c-reducing activity indicated that the wild-type and mutant flAnCYPORs followed a<br />
non-classical two-site Ping-Pong mechanism, similar to rat CYPOR. The single mutant (L86F or L219F) and double mutant<br />
(L86F/L219F) flAnCYPOR enzymes, upon reconstitution with the An. minimus cytochrome P450 CYP6AA3 and a NADPHregenerating<br />
system, increased CYP6AA3-mediated deltamethrin degradation compared to the wild-type flAnCYPOR enzyme.<br />
The increased enzyme activity could illustrate a more efficient electron transfer <strong>of</strong> AnCYPOR to CYP6AA3 cytochrome P450<br />
enzyme. Addition <strong>of</strong> extra flavin c<strong>of</strong>actors could increase CYP6AA3-mediated activity supported by wild-type and mutant<br />
flAnCYPOR enzymes. Thus, both leucine to phenylalanine substitutions are essential for flAnCYPOR enzyme in supporting<br />
CYP6AA3-mediated metabolism. © <strong>2010</strong> Wiley Periodicals, Inc.<br />
No.1217<br />
Author (s) : Sasaki, T., Hirai, I., Niki, M., Nakamura, T., Komalamisra, C., Maipanich, W., Kusolsuk, T., Sa-nguankiat, S., Pubampen, S.,<br />
Yamamoto, Y.<br />
Title : High prevalence <strong>of</strong> CTX-M β-lactamase-producing enterobacteriaceae in stool specimens obtained from healthy individuals in<br />
Thailand<br />
Source : Journal <strong>of</strong> Antimicrobial Chemotherapy, Volume 65, Issue 4, 27 January <strong>2010</strong>, Article number dkq008, Pages 666-668<br />
Document Type : Article<br />
Keywords : ESBLs; Faecal carriage; Genotypes; Rural areas<br />
<strong>Abstract</strong> : Objectives: To determine the prevalence <strong>of</strong> CTX-M β-lactamase-producing Enterobacteriaceae in stool specimens obtained<br />
from healthy individuals in a rural area <strong>of</strong> Thailand. Methods: Bacteria in stool specimens were screened for extendedspectrum<br />
β-lactamase (ESBL) production on McConkey agar with cefotaxime and confirmed by the double-disc synergy test.<br />
Genetic detection and genotyping <strong>of</strong> CTX-M-type ESBL was performed by PCR with bacterial DNA extracted from isolates.<br />
Results: A markedly high number (82 <strong>of</strong> 141, 58.2%) <strong>of</strong> the specimens showed the presence <strong>of</strong> CTX-M β-lactamase-producing<br />
Enterobacteriaceae, as confirmed by both phenotypic and genetic examinations. The majority <strong>of</strong> the CTX-M β-lactamaseproducing<br />
bacteria were Escherichia coli (85.1%). Conclusions: The study revealed the wide dissemination <strong>of</strong> CTX-M βlactamase-producing<br />
Enterobacteriaceae in the healthy population. © The Author <strong>2010</strong>. Published by Oxford <strong>University</strong> Press<br />
on behalf <strong>of</strong> the British Society for Antimicrobial Chemotherapy.<br />
No.1218<br />
Author (s) : Sasanakul, W.; Chuansumrit, A.; Kadegasem, P.; Chaiyaratana, W.<br />
Title : Simple and accurate bedside diagnostic kit for determining hemophilia A and B<br />
Source : HAEMOPHILIA, Volume: 16, Supplement: 4, Pages: 53-53, Published: JUL <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1219<br />
Author (s) : Satal<strong>of</strong>f, R.T., Praneetvatakul, P., Heuer, R.J., Hawkshaw, M.J., Heman-Ackah, Y.D., Schneider, S.M., Mandel, S.<br />
Title : Laryngeal Electromyography: Clinical Application<br />
Source : Journal <strong>of</strong> Voice, Volume 24, Issue 2, March <strong>2010</strong>, Pages 228-234<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Document Type : Article<br />
Keywords : Electromyography; Laryngeal electromyography; Vocal fold fixation; Vocal fold paralysis; Vocal fold paresis<br />
<strong>Abstract</strong> : Laryngeal electromyography (LEMG) is a valuable adjunct in clinical management <strong>of</strong> patients with voice disorders. LEMG is<br />
valuable in differentiating vocal fold paresis/paralysis from cricoarytenoid joint fixation. Our data indicate that visual<br />
assessment alone is inadequate to diagnose neuromuscular dysfunction in the larynx and that diagnoses based on vocal<br />
dynamics assessment and strobovideolaryngoscopy are wrong in nearly one-third <strong>of</strong> cases, based on LEMG results. LEMG has<br />
also proven valuable in diagnosing neuromuscular dysfunction in some dysphonic patients with no obvious vocal fold<br />
movement abnormalities observed during strobovideolaryngoscopy. Review <strong>of</strong> 751 patients suggests that there is a<br />
correlation between the severity <strong>of</strong> paresis and treatment required to achieve satisfactory outcomes; that is, LEMG allows us<br />
to predict whether patients will probably require therapy alone or therapy combined with surgery. Additional evidence-based<br />
research should be encouraged to evaluate efficacy further. © <strong>2010</strong> The Voice Foundation.<br />
No.1220<br />
Author (s) : Satangput, P., Gerdsri, N., Damrongchai, N.<br />
Title : Scenario analysis for identifying the development areas <strong>of</strong> future technologies to combat emerging infectious diseases: APEC<br />
efforts<br />
Source : 5th IEEE <strong>International</strong> Conference on Management <strong>of</strong> Innovation and Technology, ICMIT<strong>2010</strong>, art. no. 5492840, pp. 45-50.<br />
Document Type : Conference Paper<br />
Keywords : EIDs; Emerging infectious diseases; Scenario analysis; Technology roadmapping<br />
<strong>Abstract</strong> : This paper presents the key findings from the collaborative study among APEC members to address the possibilities on the<br />
future development <strong>of</strong> emerging infectious diseases and the opportunities/challenges that technologies can be effectively<br />
exploited to deal with diseases. The scenario planning technique has been applied in the study and integrated with<br />
bibliometric analysis and technology roadmapping. As a result, experts highlighted the future development <strong>of</strong> key<br />
technological domains in the areas <strong>of</strong> modeling, tracking and vaccine development. © <strong>2010</strong> IEEE.<br />
No.1221<br />
Author (s) : Sathornsumetee, S., Desjardins, A., Vredenburgh, J.J., McLendon, R.E., Marcello, J., Herndon, J.E., Mathe, A., Hamilton, M., Rich,<br />
J.N., Norfleet, J.A., Gururangan, S., Friedman, H.S., Reardon, D.A.<br />
Title : Phase II trial <strong>of</strong> bevacizumab and erlotinib in patients with recurrent malignant glioma<br />
Source : Neuro-Oncology, 12 (12), pp. 1300-1310.<br />
Document Type : Article<br />
Keywords : Antiangiogenesis; Bevacizumab; EGFR; Glioblastoma; Malignant glioma; VEGF<br />
<strong>Abstract</strong> : Vascular endothelial growth factor (VEGF) and epidermal growth factor receptor (EGFR) signaling are established contributors<br />
to malignant glioma (MG) biology. We, therefore, evaluated bevacizumab, a humanized anti-VEGF monoclonal antibody, in<br />
combination with the EGFR tyrosine kinase inhibitor erlotinib, in this phase 2 study for recurrent MG patients (www.<br />
ClinicalTrials.gov, NCT00671970). Fifty-seven patients(n 5 25, glioblastoma [GBM]; n 5 32, anaplastic glioma [AG]) were<br />
enrolled. The primary endpoint was 6-month progression-free survival (PFS-6). Overall survival (OS), radiographic response,<br />
pharmacokinetics, and correlative biomarkers were the secondary endpoints. Patients were stratified based on the concurrent<br />
use <strong>of</strong> enzyme-inducing antiepileptic drugs (EIAEDs). Bevacizumab (10 mg/kg) was given intravenously every 2 weeks. Erlotinib<br />
was orally administered daily at 200 mg/day for patients not on EIAEDs and 500 mg/day for patients on EIAEDs. PFS-6 and<br />
median OS were 28% and 42 weeks for GBM patients and 44% and 71 weeks for AG patients, respectively. Twelve (48%) GBM<br />
patients and 10 (31%) AG patients achieved a radiographic response. Erlotinib pharmacokinetic exposures were comparable<br />
between EIAED and non-EIAED groups. Rash, mucositis, diarrhea, and fatigue were common but mostly grades 1 and 2. Among<br />
GBM patients, grade 3 rash, observed in 32%, was associated with survival benefit, whereas elevated hypoxia-inducible factor-<br />
2 alpha and VEGF receptor- 2 levels were associated with poor survival. Bevacizumab plus erlotinib was adequately tolerated<br />
in recurrent MG patients. However, this regimen was associated with similar PFS benefit and radiographic response when<br />
compared with other historical bevacizumab- containing regimens. © The Author(s) <strong>2010</strong>.<br />
No.1222<br />
Author (s) : Sato, M., Pongvongsa, T., Sanguankiat, S., Yoonuan, T., Dekumyoy, P., Kalambaheti, T., Keomoungkhoun, M., Phimmayoi, I.,<br />
Boupha, B., Moji, K., Waikagul, J.<br />
Title : Copro-DNA diagnosis <strong>of</strong> Opisthorchis viverrini and Haplorchis taichui infection in an endemic area <strong>of</strong> LAO PDR<br />
445
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, 41 (1), pp. 28-35.<br />
Document Type : Article<br />
Keywords : Copro-DNA diagnosis; Haplorchis taichui; Lao PDR; Opisthorchis viverrini<br />
<strong>Abstract</strong> : The utility <strong>of</strong> differential copro-DNA diagnosis using modified sample preparation steps <strong>of</strong> small liver and minute intestinal<br />
fluke infections was tested. Fecal samples containing parasite eggs were washed extensively with diluted detergent solution.<br />
Parasite eggs were concentrated by sedimentation and broken by microwaving before DNA extraction. PCR targeting ITS1 and<br />
ITS2 regions were performed using primer specific for Opisthorchis viverrini, Haplorchis taichui and other related species. Of<br />
125 fecal samples, 94 were positive for small trematode eggs by a modified cellophane thick smear method. By ITS1-PCR, 52<br />
samples were positive for O. viverrini, 12 H. taichui and 7 mixed infection. By ITS2-PCR, 63 were positive for O. viverini, 17 H.<br />
taichui, and 19 mixed infection. The ITS-PCR assay identified a higher number <strong>of</strong> opisthorchiasis cases than those with O.<br />
viverrini expelled after treatment, but for H. taichui, ITS-PCR identified less than half <strong>of</strong> the worm expelled cases. These<br />
results showed that copro-DNA diagnosis was useful for the differential diagnosis <strong>of</strong> O. viverrini and H. taichui infection, which<br />
could not be discriminated by microscopy.<br />
No.1223<br />
Author (s) : Sato, M., Sanguankiat, S., Yoonuan, T., Pongvongsa, T., Keomoungkhoun, M., Phimmayoi, I., Boupa, B., Moji, K., Waikagul, J.<br />
Title : Copro-molecular identification <strong>of</strong> infections with hookworm eggs in rural Lao PDR<br />
Source : Transactions <strong>of</strong> the Royal Society <strong>of</strong> Tropical Medicine and Hygiene, 104 (9), pp. 617-622.<br />
Document Type : Article<br />
Keywords : Animal hookworm; Copro-PCR; Hookworm eggs; Molecular species identification; Trichostrongylus colubriformis; Zoonosis<br />
<strong>Abstract</strong> : The prevalence <strong>of</strong> hookworm eggs in fecal samples, by modified cellophane thick-smear technique, was found to be 30.0%<br />
(61/203 samples) in Lahanam Village, Savannakhet Province, Lao PDR. The hookworm eggs were morphologically<br />
heterogeneous, so that identification was confirmed by copro-PCR with specific primers for hookworms and Trichostrongylus<br />
spp.; 12 samples were positive for Necator americanus, 19 for Ancylostoma spp., with one mixed infection <strong>of</strong> both.<br />
Sequencing <strong>of</strong> the Ancylostoma spp. copro-PCR products found A. duodenale, and also the animal hookworms, A. caninum<br />
and A. ceylanicum. Moreover, Trichostrongylus spp.-specific copro-PCR revealed a significant rate <strong>of</strong> infection (43/203; 21.2%).<br />
Sequencing confirmed the zoonotic species, T. colubriformis. PCR/sequencing is useful for differentiating parasite species. The<br />
positives by the Kato-Katz method, and by the combined hookworm/. Trichostrongylus specific-PCR, were comparable,<br />
although the agreement between the two methodologies was only 50%. © <strong>2010</strong> Royal Society <strong>of</strong> Tropical Medicine and<br />
Hygiene.<br />
No.1224<br />
Author (s) : Satoh, E., Roachanakanan, R., Nishiura, S., Kishita, M.<br />
Title : A proposal for agricultural land use simulation in Thailand<br />
Source : Journal <strong>of</strong> Asian Architecture and Building Engineering, 9 (1), pp. 39-46.<br />
Document Type : Article<br />
Keywords : Agricultural land use; Cost benefit; Reduction <strong>of</strong> environmental loads; Simulation<br />
<strong>Abstract</strong> : This study considers an agricultural land use plan using multi-agent simulation in the agricultural area <strong>of</strong> Nakhon Pathom<br />
Prefecture, Thailand, where small-scale croplands <strong>of</strong> various products exist. In formulating the simulation, interviews with<br />
farmers were conducted and a research index <strong>of</strong> the area was used so that the opinion <strong>of</strong> the stakeholders will be reflected<br />
in the plan. This study is comprised <strong>of</strong> the following research and analysis. Interviews with farmers concerning future land use<br />
Field study <strong>of</strong> the size <strong>of</strong> the croplands and the kinds <strong>of</strong> products Construction <strong>of</strong> the simulation Calculation <strong>of</strong> the<br />
transportation distance, transportation cost and impact on the environment As a result <strong>of</strong> the simulation to integrate the<br />
croplands <strong>of</strong> the same products, it was found that pursuing integration to the end does not necessarily reduce the<br />
transportation cost and environmental impact. It is effective to conduct integration while paying attention to the cost<br />
effectiveness.<br />
No.1225<br />
Author (s) : Satumanatpan, S., Henocque, Y.<br />
Title : Tracking progress in coastal management-across the integrated coastal management cycle and indicators<br />
Source : EnvironmentAsia, 3 (1), pp. 39-46.<br />
Document Type : Article<br />
446
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : Assessment; Coastal management; Integrated coastal management; Project cycle<br />
<strong>Abstract</strong> : This paper presents the framework for assessing progress <strong>of</strong> coastal management initiatives. The framework is based on the<br />
cycle <strong>of</strong> integrated coastal management (ICM). Each step in the cycle suggests the indicators or self-assessment questions by<br />
which progress and learning can be assessed. We worked out and tested in the field self-assessment questions through<br />
participation techniques and meetings with a number <strong>of</strong> stakeholders among local coastal management projects in Thailand.<br />
The prime stakeholders comprise community members, local government <strong>of</strong>ficers, and coastal management managers. More<br />
senior levels <strong>of</strong> government need to be involved in the progress assessment as well, as they potentially have the capacity for<br />
making policy changes and resource allocation decisions that will aid the local stakeholders in achieving integrated coastal<br />
management.<br />
No.1226<br />
Author (s) : Sawangtong, P., Jumpen, W.<br />
Title : Blow-up solutions <strong>of</strong> degenerate parabolic problems<br />
Source : WSEAS Transactions on Mathematics, 9 (9), pp. 723-733.<br />
Document Type : Article<br />
Keywords : Blow-up set; Blow-up solution; Degenerate parabolic problems; Finite time blow-up<br />
<strong>Abstract</strong> : In this article, we study the degenerate parabolic problem, x qut-(x βu x) x = x qf(u), satisfying the Dirichlet boundary<br />
condition and a nonnegative initial condition where q and β are given constants and f is a suitable function. We show that<br />
under certain conditions the degenerate parabolic problem has a blow-up solution and the blow-up set <strong>of</strong> such a blow-up<br />
solution is the whole domain <strong>of</strong> x. Furthermore, we give the sufficient condition to blow-up in finite time. Finally, we<br />
generalize the degenerate parabolic problem into the general form, k(x)u t-(p(x)u x) x =k(x)f(u). Under appropriate assumptions<br />
on functions k, p and f, we still obtain the same results as the previous problem.<br />
No.1227<br />
Author (s) : Sawangtong, P., Jumpen, W.<br />
Title : Existence <strong>of</strong> a blow-up solution for a degenerate parabolic initial-boundary value problem<br />
Source : <strong>International</strong> Conference on Applied Mathematics, Simulation, Modelling - Proceedings, pp. 13-18.<br />
Document Type : Conference Paper<br />
Keywords : Blow-up set; Degenerate parabolic problems; Finite time blow-up<br />
<strong>Abstract</strong> : Here, before blow-up occurs, we establish the existence and uniqueness <strong>of</strong> a blow-up solution <strong>of</strong> a degenerate parabolic<br />
initial-boundary value problem,xqut-(xβux) x=xq(u) where q and β are given constants and f is a suitable function. We further<br />
give the sufficient condition to blow-up in finite time. Finally the blow-up set <strong>of</strong> a blow-up solution u is shown.<br />
No.1228<br />
Author (s) : Sawangtong, P., Novaprateep, B., Jumpen, W.<br />
Title : Complete blow-up for a degenerate semilinear parabolic problem with a localized nonlinear term<br />
Source : <strong>International</strong> Conference on Theoretical and Applied Mechanics, <strong>International</strong> Conference on Fluid Mechanics and Heat and<br />
Mass Transfer - Proceedings, pp. 94-99.<br />
Document Type : Conference Paper<br />
Keywords : Blow-up in finite time; Blow-up set; Complete blowup; Localized nonlinear terms; Semilinear parabolic problems<br />
<strong>Abstract</strong> : We here establish the local existence and uniqueness <strong>of</strong> a continuous solution under certain conditions <strong>of</strong> a degenerate<br />
semilinear parabolic problem with a localized nonlinear term: let T be any positive real number and x 0 be a fixed number in<br />
the interval (0,1), ut - 1/k(x)(p(x)ux)x = f(u(x0,t)) for (x,t) ε (0,1) × (0,T), u(0,t) = 0 = u(1,t) for t ε (0,T), u(x,0) = u 0(x) for x ε [0,1],<br />
where k, p, f and u0 are given functions. Moreover, the sufficient condition to blow-up in finite time and the blow-up set <strong>of</strong> a<br />
such solution u are shown.<br />
No.1229<br />
Author (s) : Sawasdee, N., Junking, M., Ngaojanlar, P., Sukomon, N., Ungsupravate, D., Limjindaporn, T., Akkarapatumwong, V., Noisakran, S.,<br />
Yenchitsomanus, P.-T.<br />
Title : Human kidney anion exchanger 1 interacts with adaptor-related protein complex 1 μ1A (AP-1 mu1A)<br />
Source : Biochemical and Biophysical Research Communications, 401 (1), pp. 85-91.<br />
Document Type : Article<br />
447
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : Adaptor-related protein complex 1 mu1A; Band 3; Distal renal tubular acidosis; Kidney anion exchanger 1; Protein<br />
trafficking; Protein-protein interaction<br />
<strong>Abstract</strong> : Kidney anion exchanger 1 (kAE1) mediates chloride (Cl-) and bicarbonate (HCO3-) exchange at the basolateral membrane <strong>of</strong><br />
kidney α-intercalated cells. Impaired trafficking <strong>of</strong> kAE1 leads to defect <strong>of</strong> the Cl-/HCO3- exchange at the basolateral<br />
membrane and failure <strong>of</strong> proton (H+) secretion at the apical membrane, causing a kidney disease - distal renal tubular<br />
acidosis (dRTA). To gain a better insight into kAE1 trafficking, we searched for proteins physically interacting with the Cterminal<br />
region <strong>of</strong> kAE1 (Ct-kAE1), which contains motifs crucial for intracellular trafficking, by a yeast two-hybrid (Y2H) system.<br />
An adaptor-related protein complex 1 μ1A (AP-1 mu1A) subunit was found to interact with Ct-kAE1. The interaction between<br />
either Ct-kAE1 or full-length kAE1 and AP-1 mu1A were confirmed in human embryonic kidney (HEK) 293T by coimmunoprecipitation,<br />
affinity co-purification, co-localization, yellow fluorescent protein (YFP)-based protein fragment<br />
complementation assay (PCA) and GST pull-down assay. The interacting site for AP-1 mu1A on Ct-kAE1 was found to be<br />
Y904DEV907, a subset <strong>of</strong> YXXØ motif. Interestingly, suppression <strong>of</strong> endogenous AP-1 mu1A in HEK 293T by small interfering<br />
RNA (siRNA) decreased membrane localization <strong>of</strong> kAE1 and increased its intracellular accumulation, suggesting for the first<br />
time that AP-1 mu1A is involved in the kAE1 trafficking <strong>of</strong> kidney α-intercalated cells. © <strong>2010</strong> Elsevier Inc.<br />
No.1230<br />
Author (s) : Sawasdidoln, C., Taweechaisupapong, S., Sermswan, R.W., Tattawasart, U., Tungpradabkul, S., Wongratanacheewin, S.<br />
Title : Growing Burkholderia pseudomallei in bi<strong>of</strong>ilm stimulating conditions significantly induces antimicrobial resistance<br />
Source : PLoS ONE, 5 (2), art. no. e9196, .<br />
Document Type : Article<br />
Keywords : antiinfective agent, ceftazidime, cotrimoxazole, doxycycline, flagellin, imipenem, n acylhomoserine lactone, gamma<br />
butyrolactone derivative, lipopolysaccharide; antibiotic resistance, antibiotic sensitivity, article, bacterial growth, bacterial<br />
membrane, bacterium isolate, bi<strong>of</strong>ilm, Burkholderia pseudomallei, controlled study, nonhuman, genetics, growth,<br />
development and aging, human, melioidosis, metabolism, microbiological examination, microbiology, multidrug<br />
resistance, mutation, physiology, Thailand; Bacteria (microorganisms), Burkholderia pseudomallei, Negibacteria; Acyl-<br />
Butyrolactones, Anti-Infective Agents, Bi<strong>of</strong>ilms, Burkholderia pseudomallei, Ceftazidime, Doxycycline, Drug Resistance, Multiple,<br />
Bacterial, Flagellin, Humans, Imipenem, Lipopolysaccharides, Melioidosis, Microbial Sensitivity Tests, Mutation, Soil<br />
Microbiology, Thailand, Trimethoprim-Sulfamethoxazole Combination<br />
<strong>Abstract</strong> : Background: Burkholderia pseudomallei, a Gram-negative bacterium that causes melioidosis, was reported to produce bi<strong>of</strong>ilm.<br />
As the disease causes high relapse rate when compared to other bacterial infections, it therefore might be due to the<br />
reactivation <strong>of</strong> the bi<strong>of</strong>ilm forming bacteria which also provided resistance to antimicrobial agents. However, the mechanism<br />
on how bi<strong>of</strong>ilm can provide tolerance to antimicrobials is still unclear. Methodology/Principal Findings:The change in<br />
resistance <strong>of</strong> B. pseudomallei to doxycycline, ceftazidime, imipenem, and trimethoprim/sulfamethoxazole during bi<strong>of</strong>ilm<br />
formation were measured as minimum bi<strong>of</strong>ilm elimination concentration (MBEC) in 50 soil and clinical isolates and also in<br />
capsule, flagellin, LPS and bi<strong>of</strong>ilm mutants. Almost all planktonic isolates were susceptible to all agents studied. In contrast,<br />
when they were grown in the condition that induced bi<strong>of</strong>ilm formation, they were markedly resistant to all antimicrobial<br />
agents even though the amount <strong>of</strong> bi<strong>of</strong>ilm production was not the same. The capsule and O-side chains <strong>of</strong> LPS mutants had<br />
no effect on bi<strong>of</strong>ilm formation whereas the flagellin-defective mutant markedly reduced in bi<strong>of</strong>ilm production. No alteration<br />
<strong>of</strong> LPS pr<strong>of</strong>iles was observed when susceptible form was changed to resistance. The higher amount <strong>of</strong> N-acyl homoserine<br />
lactones (AHLs) was detected in the high bi<strong>of</strong>ilm-producing isolates. Interestingly, the bi<strong>of</strong>ilm mutant which produced a very<br />
low amount <strong>of</strong> bi<strong>of</strong>ilm and was sensitive to antimicrobial agents significantly resisted those agents when grown in bi<strong>of</strong>ilm<br />
inducing condition. Conclusions/Significance:The possible drug resistance mechanism <strong>of</strong> bi<strong>of</strong>ilm mutants and other isolates is<br />
not by having bi<strong>of</strong>ilm but rather from some factors that up-regulated when bi<strong>of</strong>ilm formation genes were stimulated. The<br />
understanding <strong>of</strong> genes related to this situation may lead us to prevent B. pseudomallei bi<strong>of</strong>ilms leading to the relapse <strong>of</strong><br />
melioidosis. © <strong>2010</strong> Sawasdidoln et al.<br />
No.1231<br />
Author (s) : Scaria, J., Ponnala, L., Janvilisri, T., Yan, W., Mueller, L.A., Chang, Y.-F.<br />
Title : Analysis <strong>of</strong> ultra low genome conservation in Clostridium difficile<br />
Source : PLoS ONE, 5 (12), art. no. e15147, .<br />
Document Type : Article<br />
448
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : Clostridium toxin, genomic DNA, tetrahydr<strong>of</strong>olic acid, antiinfective agent, bacterial DNA; adaptation, antibiotic<br />
resistance, article, bacterial genome, bacterial strain, bacterial virulence, bacterium isolate, Clostridium difficile, comparative<br />
genomic hybridization, controlled study, genetic conservation, genetic variability, genome size, microarray<br />
analysis, nonhuman, nucleotide sequence, phenotype, sequence analysis, signal transduction, strain difference, toxin<br />
synthesis, animal, biological model, cattle, DNA sequence, genetics, horse, human, species difference, virulence; Clostridium<br />
difficile; Animals, Anti-Bacterial Agents, Cattle, Clostridium difficile, Comparative Genomic Hybridization, DNA, Bacterial, Drug<br />
Resistance, Bacterial, Genetic Variation, Genome, Bacterial, Horses, Humans, Models, Genetic, Sequence Analysis, DNA, Species<br />
Specificity, Virulence<br />
<strong>Abstract</strong> : Microarray-based comparative genome hybridisations (CGH) and genome sequencing <strong>of</strong> Clostridium difficile isolates have<br />
shown that the genomes <strong>of</strong> this species are highly variable. To further characterize their genome variation, we employed<br />
integration <strong>of</strong> data from CGH, genome sequencing and putative cellular pathways. Transcontinental strain comparison using<br />
CGH data confirmed the emergence <strong>of</strong> a human-specific hypervirulent cluster. However, there was no correlation between<br />
total toxin production and hypervirulent phenotype, indicating the possibility <strong>of</strong> involvement <strong>of</strong> additional factors towards<br />
hypervirulence. Calculation <strong>of</strong> C. difficile core and pan genome size using CGH and sequence data estimated that the core<br />
genome is composed <strong>of</strong> 947 to 1,033 genes and a pan genome comprised <strong>of</strong> 9,640 genes. The reconstruction, annotation and<br />
analysis <strong>of</strong> cellular pathways revealed highly conserved pathways despite large genome variation. However, few pathways<br />
such as tetrahydr<strong>of</strong>olate biosynthesis were found to be variable and could be contributing to adaptation towards virulence<br />
such as antibiotic resistance. © <strong>2010</strong> Scaria et al.<br />
No.1232<br />
Author (s) : Schechter, A.N.; Srihirun, S.; Sriwantana, T.; Unchern, S.; Noulsri, E.; Pattanapanyasat, K.; Fucharoen, S.; Piknova, B.; Sibmooh, N.<br />
Title : Platelet Aggregation Is Inhibited by Nitrite Reduction to Nitric Oxide In Blood<br />
Source : BLOOD, Volume: 116, Issue: 21, Pages: 1310-1311, Published: NOV 19 <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1233<br />
Author (s) : Schumann, J.H., Wangenheim, F.V., Stringfellow, A., Yang, Z., Blazevic, V., Praxmarer, S., Shainesh, G., Komor, M., Shannon, R.M.,<br />
Jiménez, F.R.<br />
Title : Cross-cultural differences in the effect <strong>of</strong> received word-<strong>of</strong>-mouth referral in relational service exchange<br />
Source : Journal <strong>of</strong> <strong>International</strong> Marketing, 18 (3), pp. 62-80.<br />
Document Type : Article<br />
Keywords : Consumer behavior; Culture; Service; Service quality; Word <strong>of</strong> mouth<br />
<strong>Abstract</strong> : Because <strong>of</strong> suggested beneficial effects <strong>of</strong> word-<strong>of</strong>-mouth (WOM) referral, service companies have invested large amounts <strong>of</strong><br />
money in customer referral programs as well as programs aimed at fostering positive communication among their existing<br />
customers. The question <strong>of</strong> cross-cultural differences in the effectiveness <strong>of</strong> WOM has recently gained increased prominence.<br />
The authors contribute to research on this topic by proposing a positive effect <strong>of</strong> received WOM on service quality<br />
perceptions among existing customers. Moreover, they predict that cultural values moderate this effect. They test the model<br />
on 1910 bank customers in 11 countries. The results show that received WOM has a positive effect on customer service<br />
quality perceptions. Furthermore, received WOM has a stronger effect on the evaluation <strong>of</strong> customers in high-uncertainty-<br />
avoidance than in low-uncertainty-avoidance cultures. No other cultural value is a significant moderator. The results imply<br />
that received WOM is also important to existing customers and that managers should adjust their strategy <strong>of</strong> referral<br />
marketing to match their target group's uncertainty-avoidance level. © <strong>2010</strong>, American Marketing Association.<br />
No.1234<br />
Author (s) : Schumann, J.H., Wangenheim, F.V., Stringfellow, A., Yang, Z., Praxmarer, S., Jiménez, F.R., Blazevic, V., Shannon, R.M., Shainesh,<br />
G., Komor, M.<br />
Title : Drivers <strong>of</strong> trust in relational service exchange: Understanding the importance <strong>of</strong> cross-cultural differences<br />
Source : Journal <strong>of</strong> Service Research, 13 (4), pp. 453-468.<br />
Document Type : Article<br />
Keywords : cross-cultural; pr<strong>of</strong>essional services; relationship marketing; trust<br />
449
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : Customer trust is <strong>of</strong> vital importance for relationship marketing in services. Service providers increasingly market their services<br />
globally, yet few researchers have addressed differences in customer trust across cultures. Our research fills this void by<br />
proposing a model, based on existing trust literature, that suggests the overall feeling <strong>of</strong> trust in the service provider depends<br />
on customers' beliefs about service providers' ability, benevolence, predictability, and integrity. The model, tested in a<br />
banking context with data from 2,284 customers in 11 countries, explains trust well across culturally diverse countries. The<br />
results <strong>of</strong> a hierarchical linear model, however, show that customers differ in the way they build trust in their service provider<br />
across cultures. Moderating effects <strong>of</strong> the cultural values <strong>of</strong> the target group largely explain this variation. Only the effect <strong>of</strong><br />
ability on trust is robust across countries. Global service firms should consider all four trust drivers when striving to build trust.<br />
The emphasis they put on each <strong>of</strong> these trust drivers, however, should differ across countries. When applying these principles<br />
to the design <strong>of</strong> marketing activities or market segmentation, marketing managers should collect data on the cultural values<br />
<strong>of</strong> their specific target groups in particular countries or cultural milieus. © The Author(s) <strong>2010</strong>.<br />
No.1235<br />
Author (s) : Sciortino, R., Ridarineni, N., Marjadi, B.<br />
Title : Caught between social and market considerations: A case study <strong>of</strong> Muhammadiyah charitable health services<br />
Source : Reproductive Health Matters, 18 (36), pp. 25-34.<br />
Document Type : Article<br />
Keywords : Faith-based health services; Family planning services; Indonesia; Maternal and child health care; Privatization and<br />
commercialization <strong>of</strong> services<br />
<strong>Abstract</strong> : A case study <strong>of</strong> Muhammadiyah's Islamic charitable health services in the islands <strong>of</strong> Java and Sumatra, Indonesia, was<br />
undertaken in 2008, to assess the impact <strong>of</strong> privatization <strong>of</strong> health care on this socially-oriented service provider, especially in<br />
terms <strong>of</strong> access for the poor. Findings presented here relate primarily to the effects on Muhammadiyah's maternal and child<br />
health and contraceptive services. In order to survive and thrive amidst private and public competitors, Muhammadiyah's<br />
primary care units, mostly consisting <strong>of</strong> maternal and child health centres and maternity clinics, when not closed altogether,<br />
have been directed toward providing curative hospital services, and more expensive and sometimes unnecessary treatment.<br />
A shift in the patient population away from the poor has also occurred, as market pressures transform this charitable<br />
enterprise into a commercial one, prejudicing reproductive health care and reducing access for those most in need. An<br />
improved stewardship role by government is needed to regulate the private sector, along with serious thinking about the<br />
future <strong>of</strong> primary and preventive care and health promotion, including for comprehensive reproductive health care. The<br />
neglect <strong>of</strong> these core primary care elements in Indonesia may worsen as privatization proceeds and pr<strong>of</strong>it considerations<br />
become more pressing with increased competition. © <strong>2010</strong> Reproductive Health Matters.<br />
No.1236<br />
Author (s) : Sedtheetorn, P.<br />
Title : Accurate BER analysis <strong>of</strong> downlink DS/CDMA multicellular systems in Nakagami fading<br />
Source : ECTI-CON <strong>2010</strong> - The <strong>2010</strong> ECTI <strong>International</strong> Conference on Electrical Engineering/Electronics, Computer,<br />
Telecommunications and Information Technology, art. no. 5491660, pp. 1164-1167.<br />
Document Type : Conference Paper<br />
Keywords : Accurate analysis, Additive White Gaussian noise, BER analysis, Bit-errors, Closed-form expression, DS/CDMA, Error rate, Fading<br />
levels, Gaussian approximations, Intercell interference, Intra-cell interference, Multicellular systems, Nakagami<br />
fading, Nakagami fading channel, Orthogonality; Fading (radio), Fading channels, Information technology, Optical<br />
communication, White noise; Bit error rate<br />
<strong>Abstract</strong> : This paper presents an accurate analysis <strong>of</strong> bit error rate for a downlink DS/CDMA multicellular system in Additive White<br />
Gaussian Noise (AWGN) Nakagami fading channel. Based on Improved Gaussian Approximation (IGA) technique, we propose an<br />
exact bit error rate expression. In addition, for a special case <strong>of</strong> Nakagami fading when the fading index becomes unity, we<br />
extend the analysis to include the effect <strong>of</strong> intracell interference, which is the consequence <strong>of</strong> losing orthogonality among<br />
the transmissions within the same cell. This facilitates us to introduce an explicit closed-form expression <strong>of</strong> the error rate that<br />
accounts for both intracell as well as intercell interferences. The proposed expressions are very useful for accurately<br />
investigating the impacts <strong>of</strong> orthogonality, propagational condition and severity <strong>of</strong> fading level on bit error rate.<br />
No.1237<br />
Author (s) : Seetapan, N., Bejrapha, P., Srinuanchai, W., Puttipipatkhachorn, S., Ruktanonchai, U.<br />
450
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Nondestructive rheological measurement <strong>of</strong> aqueous dispersions <strong>of</strong> solid lipid nanoparticles: Effects <strong>of</strong> lipid types and<br />
concentrations on dispersion consistency<br />
Source : Drug Development and Industrial Pharmacy, 36 (9), pp. 1005-1015.<br />
Document Type : Article<br />
Keywords : Lipid structure; Rheology; Solid lipid nanoparticles; Triglyceride; Viscoelastic<br />
<strong>Abstract</strong> : Purpose: To investigate dispersion consistency <strong>of</strong> solid lipid nanoparticles as functions <strong>of</strong> lipid types and concentrations.<br />
Methods: Viscoelastic measurement at an application <strong>of</strong> low stress was employed to characterize the internal microstructure<br />
developed within the dispersions. Pure triglycerides with different length <strong>of</strong> fatty acid chains, trimyristin (C14), tripalmitin (C16),<br />
and tristearin (C18) were studied with respect to the partial triglyceride with C22 chain length (Compritol 888 ATO), and cetyl<br />
palmitate wax (C16). Results and discussion: Increasing fatty acid chain length <strong>of</strong> triglycerides induced more particle shape<br />
anisometry; therefore, elastic behavior <strong>of</strong> triglyceride dispersion increased in sequence <strong>of</strong> trimyristin < tripalmitin < tristearin.<br />
Because <strong>of</strong> an imperfect crystalline structure, Compritol 888 ATO particles yielded the dispersion with a less elastic behavior.<br />
Despite having an equal fatty acid chain length (C16), cetyl palmitate wax provided the dispersion with lower network<br />
strength than tripalmitin as a result <strong>of</strong> the lower ordered crystal packing <strong>of</strong> fatty acid chains in the wax particle. Increasing<br />
lipid concentration improved the dispersion consistency owing to the more pronounced interaction between lipid particles.<br />
Data obtained from particle size analysis did not help explain the resulting microstructures in relation to the types and<br />
concentrations <strong>of</strong> lipid. Conclusions: A nondestructive rheological experiment is a powerful tool in revealing the microscopic<br />
structures <strong>of</strong> SLNs, which provides the information on viscous and elastic behaviors, corresponding to the internal structure <strong>of</strong><br />
the dispersions. Consequently, viscoelastic data might assist pharmaceutical industry in selecting type <strong>of</strong> lipid appropriate for<br />
developing SLN formulations with the desired consistency. © <strong>2010</strong> Informa UK, Ltd.<br />
No.1238<br />
Author (s) : Sein, M.M., Howteerakul, N., Suwannapong, N., Jirachewee, J.<br />
Title : Job strain among rubber-glove-factory workers in central Thailand<br />
Source : Industrial Health, 48 (4), pp. 503-510.<br />
Document Type : Article<br />
Keywords : Factory workers; Job strain; Occupational health; Psychosocial work factor; Rubber glove; Thailand<br />
<strong>Abstract</strong> : Job strain has become a major concern because <strong>of</strong> its potential impacts on worker well-being and performance. This crosssectional<br />
study aimed to assess the prevalence <strong>of</strong>, and examine factors associated with, job strain among workers in a rubberglove<br />
factory, in a central province <strong>of</strong> Thailand. A total <strong>of</strong> 200 workers aged 18-55 yr, who had worked at the factory for at<br />
least 6 months, completed the Job Content Questionnaire (JCQ) (Thai Version). Two <strong>of</strong> 5 scales in the JCQ were used to<br />
measure job strain, i.e.; job control and psychological job demand. The prevalence <strong>of</strong> job strain was 27.5%. Multiple logistic<br />
regression analysis indicated two variables significantly associated with job strain: low supervisor social support (adjusted<br />
OR=3.08; 95%CI: 1.38-6.91) and high job insecurity (adjusted OR=2.25; 95%CI: 1.04-.88). Effective training for supervisors, to<br />
create good relationships among workers and supervisors, and ensuring steady and secure jobs for good employees, are<br />
necessary.<br />
No.1239<br />
Author (s) : Senanarong, V.; Harnphadungkit, K.; Chawalparit, O.; Poungvarin, N.; Udomphanthurak, S.; Cummings, J.; Scheltens, P.<br />
Title : White matter lesions (WML) in a community <strong>of</strong> Thai elders with MCI and dementia<br />
Source : EUROPEAN JOURNAL OF NEUROLOGY, Volume: 17, Special Issue: SI, Supplement: 3, Pages: 76-76, Published: SEP <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1240<br />
Author (s) : Senapin, S., Molthathong, S., Phiwsaiya, K., Jaengsanong, C., Chuchird, N.<br />
Title : Application <strong>of</strong> high resolution melt (HRM) analysis for duplex detection <strong>of</strong> Macrobrachium rosenbergii nodavirus (MrNV) and<br />
extra small virus (XSV) in shrimp<br />
Source : Molecular and Cellular Probes, 24 (5), pp. 291-297.<br />
Document Type : Article<br />
Keywords : Detection; High resolution melt; HRM; MrNV; Multiplex; Nested-PCR; RT-PCR; Shrimp; Virus; XSV<br />
451
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : In this work, a probe-free, multiplex RT-PCR was combined with high resolution melt (HRM) analysis for the simultaneous<br />
detection <strong>of</strong> Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV) infection in the freshwater prawn<br />
Macrobrachium rosenbergii. This first application <strong>of</strong> HRM multiplex RT-PCR in shrimp reveals a new potential for rapid and<br />
sensitive detection <strong>of</strong> multiple pathogens. In addition, sequence variation in XSV could be observed from the high resolution<br />
melt peaks, as confirmed by sequence analysis. In 19 field samples <strong>of</strong> the freshwater prawn M. rosenbergii the technique<br />
revealed samples negative for both viruses, positive for both viruses or positive for MrNV alone. No sample was found<br />
positive for XSV alone. Comparison <strong>of</strong> these results to those obtained using the same samples in analysis by traditional<br />
nested RT-PCR combined with gel electrophoresis revealed that HRM multiplex RT-PCR was more sensitive. Thus, the latter<br />
technique allows for rapid and sensitive, simultaneous detection <strong>of</strong> MrNV and XSV and also has the potential to be adapted<br />
for simultaneous detection <strong>of</strong> other mixed viral infections in shrimp. © <strong>2010</strong> Elsevier Ltd.<br />
No.1241<br />
Author (s) : Senapin, S., Phiwsaiya, K., Anantasomboon, G., Sriphaijit, T., Browdy, C.L., Flegel, T.W.<br />
Title : Knocking down a Taura syndrome virus (TSV) binding protein Lamr is lethal for the whiteleg shrimp Penaeus vannamei<br />
Source : Fish and Shellfish Immunology, 29 (3), pp. 422-429.<br />
Document Type : Article<br />
Keywords : Laminin receptor; RNA interference; Shrimp; Taura syndrome virus; Viability<br />
<strong>Abstract</strong> : A cDNA encoding a laminin receptor protein (Lamr) has been isolated from hemocytes <strong>of</strong> the Pacific white shrimp Penaeus<br />
(Litopenaeus) vannamei (Pv), based on primers designed from a previously published Lamr sequence <strong>of</strong> a Taura syndrome<br />
virus (TSV) binding protein <strong>of</strong> the black tiger shrimp Penaeus monodon (Pm). The deduced amino acid sequence <strong>of</strong> PvLamr<br />
shares 97% identity with PmLamr and has significant homology to laminin receptors and ribosomal protein p40 from various<br />
organisms. Tissue distribution analysis by RT-PCR revealed that Lamr transcripts were widely expressed in all tested tissues <strong>of</strong><br />
P. monodon and Penaeus vannamei. PmLamr was constructed and expressed in Escherichia coli, and the recombinant<br />
protein was purified and used to raise a polyclonal antibody. The antiserum reacted with purified recombinant PmLamr and<br />
crude muscle tissue proteins from both P. monodon and P. vannamei, but not with hemocyte-free shrimp hemolymph.<br />
Examination <strong>of</strong> protein localization by immunohistochemical analysis revealed the presence <strong>of</strong> Lamr positive cytoplasm in<br />
subcuticular epithelial cells, hematopoietic tissues, epithelial cells <strong>of</strong> the stomach, epithelial cells <strong>of</strong> the anterior midgut<br />
cecum, antennal gland epithelial cells, F cells <strong>of</strong> the hepatopancreas, cells in the ovarian zone <strong>of</strong> proliferation and spheroid<br />
cells in the lymphoid organ. RNA interference-mediated silencing <strong>of</strong> the messenger from Lamr in P. vannamei led to shrimp<br />
mortality and indicated an essential function <strong>of</strong> Lamr for shrimp viability. A negative consequence was that the effect <strong>of</strong> Lamr<br />
knockdown on shrimp infection by Taura syndrome virus could not be assessed. © <strong>2010</strong> Elsevier Ltd.<br />
No.1242<br />
Author (s) : Senapin, S., Thaowbut, Y., Gangnonngiw, W., Chuchird, N., Sriurairatana, S., Flegel, T.W.<br />
Title : Impact <strong>of</strong> yellow head virus outbreaks in the whiteleg shrimp, Penaeus vannamei (boone), in Thailand<br />
Source : Journal <strong>of</strong> Fish Diseases, 33 (5), pp. 421-430.<br />
Document Type : Article<br />
Keywords : Detection; Penaeus vannamei; Shrimp; Thailand; Virus; Yellow head virus<br />
<strong>Abstract</strong> : Yellow head virus (YHV) is known as a major pathogen in the black tiger shrimp, Penaeus (Penaeus) monodon. It can also<br />
cause serious mortality in farmed whiteleg shrimp, Penaeus (Litopenaeus) vannamei. However, there is no published<br />
information on the economic and/or production impact <strong>of</strong> the disease in P. vannamei. Shrimp with gross signs <strong>of</strong> YHV disease<br />
(faded body colour and 60-70% mortality) were observed in 20 study farms rearing P. vannamei in the central part <strong>of</strong><br />
Thailand from the end <strong>of</strong> 2007 through early 2008. The estimated economic loss for these farms according to the Thai Animal<br />
Aquaculture Association was approximately US$3 million. Detailed sequence analysis <strong>of</strong> RT-PCR amplicons from shrimp in all<br />
the study ponds revealed the presence <strong>of</strong> YHV Type 1b (YHV-1b) alone (characterized by a 162-bp deletion in the ORF3<br />
region encoding the structural gene for gp116) and the absence <strong>of</strong> YHV Type 1a (YHV-1a), the original YHV type reported from<br />
Thailand. Despite the large 162-bp deletion (= 54 deduced amino acids) in the gp116 structural gene, histopathology <strong>of</strong> YHV-<br />
1b infections was identical to that <strong>of</strong> YHV-1a infections, and electron microscopy revealed that YHV-1b virions were<br />
morphologically indistinguishable from those previously reported for YHV-1a. In addition, an existing commercial RT-PCR<br />
detection kit and an immunochromatographic test strip for the detection <strong>of</strong> YHV were proven to have been valid tests for<br />
both YHV-1b and YHV-1a. The source <strong>of</strong> the virus for these outbreaks was unlikely to have been the post-larvae used to stock<br />
452
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
the ponds, as they were derived from domesticated specific pathogen-free stocks free <strong>of</strong> YHV. Thus, it is possible that they<br />
originated from an unknown, natural reservoir. © <strong>2010</strong> Blackwell Publishing Ltd.<br />
No.1243<br />
Author (s) : Senawongse, P., Pongprueksa, P., Tagami, J.<br />
Title : The effect <strong>of</strong> the elastic modulus <strong>of</strong> low-viscosity resins on the microleakage <strong>of</strong> class V resin composite restorations under<br />
occlusal loading<br />
Source : Dental Materials Journal, 29 (3), pp. 324-329.<br />
Document Type : Article<br />
Keywords : Elastic cavity wall; Elastic modulus; Microleakage<br />
<strong>Abstract</strong> : This study aimed to evaluate the effectiveness <strong>of</strong> various intermediate layers on the microleakage <strong>of</strong> Class V restorations<br />
under an occlusal load. Wedge-shaped cavities were prepared on the buccal surface <strong>of</strong> 72 extracted premolars, which were<br />
then treated with an adhesive system (One Up Bond F Plus), divided into three groups, and restored with: 1) Estelite Sigma<br />
resin composite, 2) a resin composite with Low Flow flowable composite, or 3) a resin composite with High Flow flowable<br />
composite. The specimens were subjected to a nano-indentation test to evaluate the elastic modulus <strong>of</strong> successive layers at<br />
the resin-dentine interface and were subjected to a microleakage test under either unloaded or loaded conditions. The<br />
elastic moduli were significantly different among substrates (p
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
upper right and left lateral incisors, lower molars, upper molars, upper canines, lower canines and lower central incisors. The<br />
least affected were the lower right and left lateral incisors. Factors that affected dmft were tooth brushing frequency,<br />
brushing with parental assistance or supervision, time brushing started, feeding pattern, and the type <strong>of</strong> milk, candy and<br />
sweet beverages consumption. It can be concluded that children in the studied area had a high prevalence <strong>of</strong> caries and a<br />
high level <strong>of</strong> severity. Thus, oral health care programs should be promoted in Vientiane.<br />
No.1246<br />
Author (s) : Sereenonchai, K., Teerasong, S., Chan-Eam, S., Saetear, P., Choengchan, N., Uraisin, K., Amornthammarong, N., Motomizu, S.,<br />
Nacapricha, D.<br />
Title : A low-cost method for determination <strong>of</strong> calcium carbonate in cement by membraneless vaporization with capacitively<br />
coupled contactless conductivity detection<br />
Source : Talanta, 81 (3), pp. 1040-1044.<br />
Document Type : Article<br />
Keywords : Carbon dioxide; Carbonate analysis; Cement; Contactless conductivity; Membraneless vaporization<br />
<strong>Abstract</strong> : This work presents a flow analysis method for direct quantitation <strong>of</strong> calcium carbonate in cement without pretreatment <strong>of</strong><br />
the sample. The method is based on online vaporization <strong>of</strong> CO2 gas following acidification <strong>of</strong> the sample inside a small<br />
chamber that has a flow <strong>of</strong> acceptor solution passing around it. Solubilization <strong>of</strong> the CO2 gas into the acceptor stream<br />
changes the conductivity <strong>of</strong> the acceptor solution causing an increase <strong>of</strong> signal at the capacitively coupled contactless<br />
conductivity detection (C4D) placed at the outlet <strong>of</strong> the vaporization chamber. This chamber is an adaption from previous<br />
work reported on 'membraneless vaporization' (MBL-VP). The method can be used in the quality control <strong>of</strong> production <strong>of</strong><br />
mixed cement. These cement materials usually have calcium carbonate contents at high concentration range (e.g., 33-99%<br />
(w/w) CaCO3). Analysis <strong>of</strong> samples by this method is direct and convenient as it requires no sample pretreatment. The<br />
method is low-cost with satisfactory accuracy and acceptable precision. © <strong>2010</strong> Elsevier B.V.<br />
No.1247<br />
Author (s) : Seripienlert, A., Ruffolo, D., Matthaeus, W.H., Chuychai, P.<br />
Title : Dropouts in solar energetic particles: Associated with local trapping boundaries or current sheets?<br />
Source : Astrophysical Journal Letters, 711 (2), pp. 980-989.<br />
Document Type : Article<br />
Keywords : Interplanetary medium; Magnetic fields; Magnetohydrodynamics (MHD); Sun: particle emission; Turbulence<br />
<strong>Abstract</strong> : In recent observations by the Advanced Composition Explorer, the intensity <strong>of</strong> solar energetic particles exhibits sudden, large<br />
changes known as dropouts. These have been explained in terms <strong>of</strong> turbulence or a flux tube structure in the solar wind.<br />
Dropouts are believed to indicate filamentary magnetic connection to a localized particle source near the solar surface, and<br />
computer simulations <strong>of</strong> a random-phase model <strong>of</strong> magnetic turbulence have indicated a spatial association between<br />
dropout features and local trapping boundaries (LTBs) defined for a two-dimensional (2D) + slab model <strong>of</strong> turbulence.<br />
Previous observations have shown that dropout features are not well associated with sharp magnetic field changes, as might<br />
be expected in the flux tube model. Random-phase turbulence models do not properly treat sharp changes in the magnetic<br />
field, such as current sheets, and thus cannot be tested in this way. Here, we explore the properties <strong>of</strong> a more realistic<br />
magnetohydrodynamic (MHD) turbulence model (2D MHD), in which current sheets develop and the current and magnetic<br />
field have characteristic non-Gaussian statistical properties. For this model, computer simulations that trace field lines to<br />
determine magnetic connection from a localized particle source indicate that sharp particle gradients should frequently be<br />
associated with LTBs, sometimes with strong 2D magnetic fluctuations, and infrequently with current sheets. Thus, the 2D<br />
MHD + slab model <strong>of</strong> turbulent fluctuations includes some realistic features <strong>of</strong> the flux tube view and is consistent with the<br />
lack <strong>of</strong> an observed association between dropouts and intense magnetic fields or currents. © <strong>2010</strong>. The American<br />
Astronomical Society.<br />
No.1248<br />
Author (s) : Seriwatanachai, D.; Krishnamra, N.; Leeuwen, J.<br />
Title : PROLACTIN REGULATES OSTEOBLAST AND OSTEOCLAST INTERACTION IN BONE REMODELING<br />
Source : OSTEOPOROSIS INTERNATIONAL, Volume: 21, Supplement: 5, Pages: S730-S730, Published: DEC <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
454
<strong>Abstract</strong> : –<br />
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.1249<br />
Author (s) : Sermsri, W., Jarujamrus, P., Shiowatana, J., Siripinyanond, A.<br />
Title : Flow field-flow fractionation: A versatile approach for size characterization <strong>of</strong> α-tocopherol-induced enlargement <strong>of</strong> gold<br />
nanoparticles<br />
Source : Analytical and Bioanalytical Chemistry, 396 (8), pp. 3079-3085.<br />
Document Type : Article<br />
Keywords : α-Tocopherol; Field-flow fractionation; Gold nanoparticles; Particle size<br />
<strong>Abstract</strong> : Flow field-flow fractionation (FlFFF) was used for size characterization <strong>of</strong> gold nanoparticles. The measured particle sizes<br />
obtained from FlFFF for the commercial 10 nm gold nanoparticle standard and the gold nanoparticles synthesized in the<br />
laboratory were in good agreement with those measured by transmission electron microscopy (TEM). Further, the capability<br />
<strong>of</strong> α-tocopherol to induce enlargement <strong>of</strong> gold nanoparticles by catalysis <strong>of</strong> the reduction <strong>of</strong> AuCl 4- by citrate was observed<br />
by monitoring the changes in particle size <strong>of</strong> gold nanoparticles using FlFFF. The effects <strong>of</strong> α-tocopherol and incubation time<br />
on enlargement <strong>of</strong> the gold nanoparticles were examined. Higher concentrations <strong>of</strong> α-tocopherol resulted in larger<br />
nanoparticles. At fixed α-tocopherol concentration, larger nanoparticles were formed at longer incubation times. © <strong>2010</strong><br />
Springer-Verlag.<br />
No.1250<br />
Author (s) : Setou, M., Shrivas, K., Sroyraya, M., Yang, H., Sugiura, Y., Moribe, J., Kondo, A., Tsutsumi, K., Kimura, Y., Kurabe, N., Hayasaka, T.,<br />
Goto-Inoue, N., Zaima, N., Ikegami, K., Sobhon, P., Konishi, Y.<br />
Title : Developments and applications <strong>of</strong> mass microscopy<br />
Source : Medical Molecular Morphology, 43 (1), pp. 1-5.<br />
Document Type : Review<br />
Keywords : Biomolecules; Clinical samples; Imaging mass spectrometry; Mass microscope; Matrix-assisted laser desorption/ionization<br />
(MALDI); Nanoparticle-based laser desorption/ionization (nano-PALDI)<br />
<strong>Abstract</strong> : We have developed a mass microscopy technique, i.e., a microscope combined with high-resolution matrixassisted laser<br />
desorption/ionization-imaging mass spectrometry (MALDI-IMS), which is a powerful tool for investigating the spatial distribution<br />
<strong>of</strong> biomolecules without any time-consuming extraction, purification, and separation procedures for biological tissue sections.<br />
Mass microscopy provides clear images about the distribution <strong>of</strong> hundreds <strong>of</strong> biomolecules in a single measurement and also<br />
helps in understanding the cellular pr<strong>of</strong>ile <strong>of</strong> the biological system. The sample preparation and the spatial resolution and<br />
speed <strong>of</strong> the technique are all important steps that affect the identification <strong>of</strong> biomolecules in mass microscopy. In this<br />
Award Lecture Review, we focus on some <strong>of</strong> the recent developments in clinical applications to show how mass microscopy<br />
can be employed to assess medical molecular morphology. © The Japanese Society for Clinical Molecular Morphology <strong>2010</strong>.<br />
No.1251<br />
Author (s) : Seubsman, S.-A., Lim, L.L.-Y., Banwell, C., Sripaiboonkit, N., Kelly, M., Bain, C., Sleigh, A.C.<br />
Title : Socioeconomic status, sex, and obesity in a large national cohort <strong>of</strong> 15-87-year-old Open <strong>University</strong> students in Thailand<br />
Source : Journal <strong>of</strong> Epidemiology, 20 (1), pp. 13-20.<br />
Document Type : Article<br />
Keywords : Body mass index; Obesity; Socioeconomic status; Thailand; Weight<br />
<strong>Abstract</strong> : Background: As obesity increases, middle-income countries are undergoing a health-risk transition. We examine the association<br />
between socioeconomic status (SES) and emerging obesity in Thailand, and ascertain if an inverse relationship between SES<br />
and obesity has appeared. Methods: The data derived from 87 134 individuals (54% female; median age, 29 years) in a<br />
national cohort <strong>of</strong> distance-learning Open <strong>University</strong> students aged 15-87 years and living throughout Thailand. We calculated<br />
adjusted odds ratios for associations <strong>of</strong> SES with obesity (body mass index, ≥25) across 3 age groups by sex, after controlling<br />
for marital status, age, and urbanization. Results: Obesity increased with age and was more prevalent among males than<br />
females (22.7% vs 9.9%); more females were underweight (21.8% vs 6.2%). Annual income was 2000 to 3000 US dollars for<br />
most participants. High SES, defined by education, income, household assets, and housing type, associated strongly with<br />
obesity-positively for males and inversely for females-especially for participants younger than 40 years. The OR for obesity<br />
associated with income was as high as 1.54 for males and as low as 0.68 for females (P for trend
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
between SES and obesity in females. We expect the overall population <strong>of</strong> Thailand to follow this pattern, as education<br />
spreads and incomes rise. A public health problem <strong>of</strong> underweight females could emerge. Recognition <strong>of</strong> these patterns is<br />
important for programs combating obesity. Many middle income countries are undergoing similar transitions. © 2009 by the<br />
Japan Epidemiological Association.<br />
No.1252<br />
Author (s) : Sheehan, W.J., Rangsithienchai, P.A., Baxi, S.N., Gardynski, A., Bharmanee, A., Israel, E., Phipatanakul, W.<br />
Title : Age-specific prevalence <strong>of</strong> outdoor and indoor aeroallergen sensitization in boston<br />
Source : Clinical Pediatrics, 49 (6), pp. 579-585.<br />
Document Type : Article<br />
Keywords : Allergy; Atopy; Children; Skin prick test<br />
<strong>Abstract</strong> : Questions exist regarding the appropriate age for referral <strong>of</strong> an atopic child to an allergist for environmental skin prick testing.<br />
This study evaluates age-specific prevalence <strong>of</strong> sensitization to aeroallergens from infancy through adolescence. A total <strong>of</strong><br />
1394 patients were skin tested, with 57.2% being sensitized to at least 1 aeroallergen. In children younger than 2, the authors<br />
found that 26.5% were sensitized, including to dogs (15.5%) and cats (9.2%). Additionally, tree sensitization was demonstrated<br />
in the youngest age group (7.8% at 0-2 years; 17.1% at 2-4 years), including in 3 infants less than 1 year old. Sensitization rates<br />
to dust mites and trees were the highest in all ages above 4 years, with a peak tree sensitization <strong>of</strong> 56.4% at 10 to 12 years<br />
and a peak dust mite sensitization <strong>of</strong> 56.8% in the >12 group. Overall, the authors observed increasing sensitization rates<br />
throughout childhood for indoor and outdoor aeroallergens (P
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Document Type : Article<br />
Keywords : foam cells; granuloma formation; Schistosoma mekongi<br />
<strong>Abstract</strong> : A histopathological study was performed to clarify the characteristics <strong>of</strong> granuloma formation and liver fibrosis in Schistosoma<br />
mekongi infection in comparison with S. japonicum infection. Mice were exposed to S. mekongi (Laotian strain) and S.<br />
japonicum (Japanese strain) cercariae, and were dissected at 6, 8, 12, 16, and 20 weeks post-exposure. In the liver,<br />
granulomas in S. mekongi infection were cellular, initially organized with foam cells, and continuously appeared in the<br />
intralobular area, while granulomas in S. japonicum infection were fibrous and did not continuously appear in the intralobular<br />
area. Portal fibrosis was not seen in S. mekongi infection, but was commonly seen in S. japonicum infection in the later<br />
weeks. Granulomas in the small intestine were seen mainly in the submucosa with foam cells in S. mekongi infection and<br />
without foam cells in S. japonicum infection. The lung granulomas contained mainly histiocytes in both S. mekongi and S.<br />
japonicum infection. The absence <strong>of</strong> portal fibrosis in S. mekongi infection allows schistosome eggs to infiltrate into the<br />
intralobular area continuously, which can be what lies behind the ultrasonographic differences; the echogenic network<br />
pattern as was seen in S. japonicum infection, has not been noted in S. mekongi infection. © Cambridge <strong>University</strong> Press <strong>2010</strong>.<br />
No.1255<br />
Author (s) : Shivakoti, B.R., Tanaka, S., Fujii, S., Kunacheva, C., Boontanon, S.K., Musirat, C., Seneviratne, S.T.M.L.D., Tanaka, H.<br />
Title : Occurrences and behavior <strong>of</strong> perfluorinated compounds (PFCs) in several wastewater treatment plants (WWTPs) in Japan and<br />
Thailand<br />
Source : Journal <strong>of</strong> Environmental Monitoring, 12 (6), pp. 1255-1264.<br />
Document Type : Article<br />
Keywords : activated carbon, perfluoro compound; activated sludge, adsorption, aeration, article, chlorination, Japan, ozonation, priority<br />
journal, tank, Thailand, waste water management; Alkanesulfonic Acids, Environmental<br />
Monitoring, Fluorocarbons, Japan, Octanoic Acids, Thailand, Waste Disposal, Fluid, Water Pollutants, Chemical<br />
<strong>Abstract</strong> : This study examines occurrences <strong>of</strong> 11 perfluorinated compounds (PFCs) in several wastewater treatment plants in Japan and<br />
Thailand. Surveys are conducted in eight wastewater treatment plants (WWTPs) in Japan and central WWTPs <strong>of</strong> five industrial<br />
estates (IEs) in Thailand. Samples are collected from all major treatment processes in order to understand the behavior <strong>of</strong><br />
PFCs in WWTPs. PFCs are detected in all WWTPs in Japan and Thailand. Concentrations <strong>of</strong> PFCs even exceed several<br />
thousands ng/L in some WWTPs. PFOS, PFOA, and PFNA are mainly detected in WWTPs in Japan, while PFBuS, PFOA, and<br />
PFHxA are mainly detected in WWTP <strong>of</strong> IEs in Thailand. Even though some <strong>of</strong> the investigated WWTPs utilize biological<br />
treatment processes coupled with chlorination, ozonation, or activated carbon adsorption, they are found ineffective to<br />
remove PFCs. During the treatment process, PFCs are found to accumulate at exceptionally high concentration levels in the<br />
activated sludge <strong>of</strong> an aeration tank and returned activated sludge. Overall, the estimated total daily mass <strong>of</strong> discharged PFCs<br />
is 124.95 g/d (PFASs: 49.81 g/d; PFCAs: 75.14 g/d) from eight WWTPs in Japan and 55.04 g/d (PFASs: 12 g/d; PFCAs: 43.04 g/d)<br />
from five WWTPs in Thailand. Although the presented data are from a single observation in each WWTP, the results indicate<br />
that certain industries using PFCs in manufacturing processes could be the principle point source, while domestic activities<br />
could be releasing PFCs at detectable levels causing environmental concern. © <strong>2010</strong> The Royal Society <strong>of</strong> Chemistry.<br />
No.1256<br />
Author (s) : Shukla, M.; Mukda, S.; Sotthibundhu, A.; Wongchitrat, P.; Piyarat, G.<br />
Title : Melatonin prevents methamphetamine-induced reduction <strong>of</strong> VMAT2 protein and mRNA level in SK-N-SH cell line<br />
Source : JOURNAL OF NEUROCHEMISTRY, Volume: 115, Supplement: 1, Pages: 83-83, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1257<br />
Author (s) : Sihabut, T., Laemsak, N.<br />
Title : Feasibility <strong>of</strong> producing insulation boards from oil palm fronds and empty fruit bunches<br />
Source : Songklanakarin Journal <strong>of</strong> Science and Technology, 32 (1), pp. 63-69.<br />
Document Type : Article<br />
Keywords : Empty fruit bunch; Frond; Insulation board; Oil palm; Thermal conductivity<br />
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<strong>Abstract</strong> : This research focused on the feasibility <strong>of</strong> producing insulation boards made from oil palm fronds and empty fruit bunches<br />
via the wet forming process. Results confirmed no difference in the visual appearance between both board types. Both<br />
displayed low thermal conductivity, <strong>of</strong>fering evidence <strong>of</strong> being good insulators. Boards made from empty fruit bunch weighed<br />
less than boards made from oil palm fronds. Other properties such as fire retardant, water absorption and strength still need<br />
to be investigated.<br />
No.1258<br />
Author (s) : Sim, B.M.Qi; Chantratita, N.; Ooi, W.F.; Nandi, T.; Tewhey, R.; Wuthiekanun, V.; Thaipadungpanit, J.; Tumapa, S.; Ariyaratne, P.;<br />
Sung, Wing-Kin; S., Xiao H.; Chua, H.H.; Ramnarayanan, K.; Lin, C.H.; Liu, Y.; Feil, E.J.; Glass, M.B.; Tan, G.; Peacock, S.J.; Tan, P.<br />
Title : Genomic acquisition <strong>of</strong> a capsular polysaccharide virulence cluster by non- pathogenic Burkholderia isolates<br />
Source : GENOME BIOLOGY, Volume: 11, Issue: 8, Article Number: R89, DOI: 10.1186/gb-<strong>2010</strong>-11-8-r89, Published: <strong>2010</strong><br />
Document Type : Article<br />
Keywords : III SECRETION SYSTEM; SIGNATURE-TAGGED MUTAGENESIS; TIME PCR ASSAY; RAPID IDENTIFICATION; BACTERIAL VIRULENCE;<br />
SUBTRACTIVE HYBRIDIZATION; PSEUDOMALLEI INFECTION; MONOCLONAL-ANTIBODY; GENES; THAILANDENSIS<br />
<strong>Abstract</strong> : Background: Burkholderia thailandensis is a non-pathogenic environmental saprophyte closely related to Burkholderia<br />
pseudomallei, the causative agent <strong>of</strong> the <strong>of</strong>ten fatal animal and human disease melioidosis. To study B. thailandensis<br />
genomic variation, we pr<strong>of</strong>iled 50 isolates using a pan-genome microarray comprising genomic elements from 28 Burkholderia<br />
strains and species. Results: Of 39 genomic regions variably present across the B. thailandensis strains, 13 regions<br />
corresponded to known genomic islands, while 26 regions were novel. Variant B. thailandensis isolates exhibited isolated<br />
acquisition <strong>of</strong> a capsular polysaccharide biosynthesis gene cluster (B. pseudomallei-like capsular polysaccharide) closely<br />
resembling a similar cluster in B. pseudomallei that is essential for virulence in mammals; presence <strong>of</strong> this cluster was<br />
confirmed by whole genome sequencing <strong>of</strong> a representative variant strain (B. thailandensis E555). Both whole-genome<br />
microarray and multi-locus sequence typing analysis revealed that the variant strains formed part <strong>of</strong> a phylogenetic subgroup<br />
distinct from the ancestral B. thailandensis population and were associated with atypical isolation sources when compared to<br />
the majority <strong>of</strong> previously described B. thailandensis strains. In functional assays, B. thailandensis E555 exhibited several B.<br />
pseudomallei-like phenotypes, including colony wrinkling, resistance to human complement binding, and intracellular<br />
macrophage survival. However, in murine infection assays, B. thailandensis E555 did not exhibit enhanced virulence relative to<br />
other B. thailandensis strains, suggesting that additional factors are required to successfully colonize and infect mammals.<br />
Conclusions: The discovery <strong>of</strong> such novel variant strains demonstrates how unbiased genomic surveys <strong>of</strong> nonpathogenic<br />
isolates can reveal insights into the development and emergence <strong>of</strong> new pathogenic species.<br />
No.1259<br />
Author (s) : Sindhu, S., Pholpet, C., Puttapitukpol, S.<br />
Title : Meeting the challenges <strong>of</strong> chronic illness: A nurse-led collaborative community care program in Thailand<br />
Source : Collegian, 17 (2), pp. 93-99.<br />
Document Type : Article<br />
Keywords : Chronic heart failure; Chronic illness; Community care; Continuing care; Coronary heart disease<br />
<strong>Abstract</strong> : Background: Chronic illness is <strong>of</strong> concern to health care systems globally. Although a significant evidence base supports the<br />
concept <strong>of</strong> nurse-led interventions, less data is available to address unique features <strong>of</strong> health care systems in the developing<br />
world. Aim: The purpose <strong>of</strong> this study aimed to undertake preliminary testing <strong>of</strong> an intervention <strong>of</strong> nurse-led community care<br />
program, the Network Collaborative Action Plan (N-CAP), to assess the impact on disease severity and patient satisfaction.<br />
Method: A quasi-experimental study, using historical controls, evaluated a collaborative nurse-led intervention to promote<br />
coordination and continuity <strong>of</strong> care for patients with chronic illness. Results: Participants, diagnosed with chronic obstructive<br />
lung disease (COPD), coronary heart disease (CHD) and chronic heart failure (CHF) were recruited. Prospective consecutive<br />
patient meeting the study criteria (n= 47) were assigned into the control group and following development and<br />
implementation <strong>of</strong> the intervention eligible consenting patients were enrolled in the experimental group (n= 44). Participants<br />
in the experimental group had significantly lower scores on severity <strong>of</strong> disease measurements during the third week (F= 4.61,<br />
p= 0.035) and the eighth week hospital (F= 4.30, p= .041) following hospital discharge than those in the control group.<br />
Participants in the experimental group expressed significantly higher scores on satisfaction with community care than those in<br />
the control group. Conclusions: A nurse-led, collaboratively developed program has potential to improve satisfaction and<br />
decrease symptom development in people with chronic illnesses in Thailand. © <strong>2010</strong> Royal College <strong>of</strong> Nursing, Australia.<br />
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No.1260<br />
Author (s) : Singalavanija, A., Amornrattanapan, C., Nitiruangjarus, K., Tongsai, S.<br />
Title : Risk factors for retinal breaks in patients with symptom <strong>of</strong> floaters<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, 93 (6), pp. 708-713.<br />
Document Type : Article<br />
Keywords : Flashing; Floaters; Posterior vitreous detachment; Retinal break; Risk factors<br />
<strong>Abstract</strong> : Objective: To identify the risk factors <strong>of</strong> retinal breaks in patients with the symptom <strong>of</strong> floaters, and to determine the<br />
association between those risk factors and retinal breaks. Material and Method: A retrospective analytic study <strong>of</strong> 184 patients<br />
(55 males and 129 females) that included 220 eyes was conducted. Patient information such as age, symptoms (multiple<br />
floaters, flashing), duration <strong>of</strong> symptom, refractive error, history <strong>of</strong> cataract surgery, family history <strong>of</strong> retinal detachment, and<br />
complete eye examination were recorded. The patients were divided into two groups, the first group (control group) had<br />
symptoms <strong>of</strong> floaters and no retinal breaks, the second group (retinal breaks group) had symptoms <strong>of</strong> floaters with retinal<br />
breaks. Chi-square test, and the multiple logistic regression were used for statistical analysis. Results: Two hundred twenty<br />
eyes, 175 eyes <strong>of</strong> the control group and 45 eyes <strong>of</strong> the retinal breaks group were examined and included in this study. The<br />
multiple logistic regression analysis revealed that patients with multiple floaters, and floaters and flashing increased the risk<br />
<strong>of</strong> retinal breaks to 5.8 and 4.3 times, respectively, when compared to patients with single floater or floaters alone. Lattice<br />
degeneration increased the risk <strong>of</strong> retinal breaks to 5.9 times when compared to eyes that did not have lattice degeneration.<br />
Conclusion: Multiple floaters, flashing and lattice degeneration are risk factors <strong>of</strong> retinal breaks in patients with symptoms <strong>of</strong><br />
floaters. Therefore, it is important for the ophthalmologists to be aware <strong>of</strong> these risk factors and the patients at risk should<br />
have follow-up examinations.<br />
No.1261<br />
Author (s) : Singh, S.B., Ornatowski, W., Vergne, I., Naylor, J., Delgado, M., Roberts, E., Ponpuak, M., Master, S., Pilli, M., White, E., Komatsu,<br />
M., Deretic, V.<br />
Title : Human IRGM regulates autophagy and cell-autonomous immunity functions through mitochondria<br />
Source : Nature Cell Biology, 12 (12), pp. 1154-1165.<br />
Document Type : Article<br />
Keywords : cardiolipin, guanosine triphosphatase, immunity related guanosine triphosphatase, protein Bak, protein Bax, protein<br />
IRGM, protein IRGMb, protein IRGMd, unclassified drug; animal cell, animal cell culture, article, autophagy, binding affinity, cell<br />
death, cell fractionation, cell membrane depolarization, cellular distribution, cellular immunity, controlled study, enzyme<br />
activity, gene overexpression, genetic transfection, human, human cell, human cell<br />
culture, mitochondrion, mouse, nonhuman, polarization, priority journal, protein analysis, protein localization;<br />
Animals, Autophagy, Cardiolipins, Cell Line, GTP Phosphohydrolases, GTP-Binding Proteins, Humans, Mice, Microtubule-<br />
Associated Proteins, Mitochondria, Mitochondrial Proteins, Protein Is<strong>of</strong>orms; Corynebacterineae<br />
<strong>Abstract</strong> : IRGM, a human immunity-related GTPase, confers autophagic defence against intracellular pathogens by an unknown<br />
mechanism. Here, we report an unexpected mode <strong>of</strong> IRGM action. IRGM demonstrated differential affinity for the<br />
mitochondrial lipid cardiolipin, translocated to mitochondria, affected mitochondrial fission and induced autophagy.<br />
Mitochondrial fission was necessary for autophagic control <strong>of</strong> intracellular mycobacteria by IRGM. IRGM influenced<br />
mitochondrial membrane polarization and cell death. Overexpression <strong>of</strong> IRGMd, but not IRGMb splice is<strong>of</strong>orms, caused<br />
mitochondrial depolarization and autophagy-independent, but Bax/Bak-dependent, cell death. By acting on mitochondria,<br />
IRGM confers autophagic protection or cell death, explaining IRGM action both in defence against tuberculosis and in the<br />
damaging inflammation caused by Crohn's disease. © <strong>2010</strong> Macmillan Publishers Limited. All rights reserved.<br />
No.1262<br />
Author (s) : Singhakant, C., Tipprasertsin, K., Inthorn, D.<br />
Title : The possible mechanism and factors affecting synthetic reactive dye removal by treated flute reed<br />
Source : EnvironmentAsia, 3 (2), pp. 90-96.<br />
Document Type : Article<br />
Keywords : Adsorption; Adsorption isotherm; Desorption; Reactive dye; Treated flute-reed<br />
<strong>Abstract</strong> : This study investigated the ability <strong>of</strong> treated flute-reed to adsorb synthetic reactive dye solution in a batch system. The<br />
effects <strong>of</strong> particle size, contact time and adsorption isotherms at various particle sizes and temperatures were investigated.<br />
Desorption was studied to confirm the mechanism <strong>of</strong> adsorption. The results showed that the adsorption capacity increased<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
as the particle size decreased. The smaller particle size required less contact time to reach equilibrium because it had a<br />
higher rate <strong>of</strong> adsorption. Adsorption isotherms at various particle sizes indicated that the equilibrium data fitted well with<br />
the Langmuir isotherm. The maximum adsorption capacity (qmax) <strong>of</strong> particle size ranges <strong>of</strong>: less than 420, 420-1190, 1190-<br />
2000 and 2000-2800 μm, was 7.58, 4.42, 3.83 and 3.12 mg/g, respectively. For adsorption isotherms at various temperatures,<br />
the amounts <strong>of</strong> adsorption increased with increasing temperature. This is because the kinetic energy probably increased at<br />
higher temperatures resulting in increasing reactive dye removal. The results from the desorption studies confirmed that the<br />
adsorption <strong>of</strong> reactive dye by treated flute-reed was due to ion exchange and the adsorption mechanism was mainly<br />
chemical adsorption.<br />
No.1263<br />
Author (s) : Shukla, M.; Mukda, S.; Sotthibundhu, A.; Wongchitrat, P.; Piyarat, G.<br />
Title : Melatonin prevents methamphetamine-induced reduction <strong>of</strong> VMAT2 protein and mRNA level in SK-N-SH cell line<br />
Source : JOURNAL OF NEUROCHEMISTRY, Volume: 115, Supplement: 1, Pages: 81-81, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1264<br />
Author (s) : Singhto, N., Sintiprungrat, K., Sinchaikul, S., Chen, S.-T., Thongboonkerd, V.<br />
Title : Proteome changes in human monocytes upon interaction with calcium oxalate monohydrate crystals<br />
Source : Journal <strong>of</strong> Proteome Research, 9 (8), pp. 3980-3988.<br />
Document Type : Article<br />
Keywords : calcium oxalate; CaOx; COM; monocytes; proteome; proteomics<br />
<strong>Abstract</strong> : Monocytic infiltration in renal interstitium is commonly found surrounding the site <strong>of</strong> calcium oxalate (CaOx) crystal<br />
deposition in the kidney. Monocytes are supposed to eliminate the deposited crystals. However, effects <strong>of</strong> CaOx crystals on<br />
the infiltrating monocytes remain unknown. Therefore, this study investigated the altered cellular proteome <strong>of</strong> human<br />
monocytes in response to interaction with CaOx monohydrate (COM) crystals. After 24-h culture with or without 100 μg/mL<br />
COM crystals, U937 cells were harvested and subjected to 2-DE analysis with Deep Purple fluorescence staining (n = 5<br />
gels/group; each was derived from independent culture). Spot matching, quantitative intensity analysis, and statistics revealed<br />
22 differentially expressed proteins (9 up-regulated and 13 down-regulated proteins), which were successfully identified by Q-<br />
TOF MS and MS/MS analyses, including those involved in cell cycle, cellular structure, carbohydrate metabolism, lipid<br />
metabolism, mRNA processing, and protein synthesis, stabilization, and degradation. Randomly selected changes [upregulated<br />
ALG-2 interacting protein 1 (Alix), elongation factor-2 (EF-2), and down-regulated β-actin] were confirmed by<br />
Western blot analysis. Our data may help to understand how monocytes interact with COM crystals. These processes are<br />
proposed to cause subsequent inflammatory response in kidney stone disease through oxidative stress pathway(s). © <strong>2010</strong><br />
American Chemical Society.<br />
No.1265<br />
Author (s) : Singkaravanit, S., Kinoshita, H., Ihara, F., Nihira, T.<br />
Title : Cloning and functional analysis <strong>of</strong> the second geranylgeranyl diphosphate synthase gene influencing helvolic acid biosynthesis<br />
in Metarhizium anisopliae<br />
Source : Applied Microbiology and Biotechnology, 87 (3), pp. 1077-1088.<br />
Document Type : Article<br />
Keywords : Geranylgeranyl diphosphate synthase; Helvolic acid; Metarhizium anisopliae; Secondary metabolism<br />
<strong>Abstract</strong> : A gene (ggs2) having high similarity to the geranylgeranyl diphosphate synthase (GGPP synthase) gene was cloned from<br />
Metarhizium anisopliae NAFF635007. The ggs2 gene (1,239-bp open reading frame with no intron) encoded a protein <strong>of</strong> 412<br />
amino acids, and the transcription occurred only after late log-phase during the growth. Gene disruption <strong>of</strong> ggs2, performed to<br />
clarify the function in M. anisopliae, resulted in decreased GGPP synthase activity together with a slight delay <strong>of</strong> sporulation.<br />
An high performance liquid chromatography (HPLC) comparison <strong>of</strong> compound pr<strong>of</strong>iles between the wild-type strain and the<br />
disruptant revealed that a compound was abolished by the ggs2 disruption. Purification and structural elucidation by 1H-NMR<br />
and mass spectrometry analyses revealed that the lost compound is helvolic acid. Furthermore, the pathogenic- ity assay<br />
against two species <strong>of</strong> insect larvae revealed that the ggs2-disruptant possessed much weaker toxicity than the wild-type<br />
460
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
strain. Based on these results, it was concluded that ggs2 encodes the GGPP synthase influencing the biosynthesis <strong>of</strong><br />
secondary metabolites in various species, including helvolic acid in M. anisopliae. To the best <strong>of</strong> our knowledge, this is the<br />
first report to identify a GGPP synthase gene related to secondary metabolism in entomopathogenic fungi. © Springer-Verlag<br />
<strong>2010</strong>.<br />
No.1266<br />
Author (s) : Singkaravanit, S., Kinoshita, H., Ihara, F., Nihira, T.<br />
Title : Geranylgeranyl diphosphate synthase genes in entomopathogenic fungi<br />
Source : Applied Microbiology and Biotechnology, 85 (5), pp. 1463-1472.<br />
Document Type : Article<br />
Keywords : Geranylgeranyl diphosphate synthase; Metarhizium anisopliae<br />
<strong>Abstract</strong> : Based on comparative amino-acid sequence alignment <strong>of</strong> geranylgeranyl diphosphate (GGPP) synthase from filamentous fungi,<br />
degenerated oligonucleotide primers were designed for searching GGPP synthase gene(s) in entomopathogenic fungi.<br />
Polymerase chain reaction with the designed primers amplified GGPP synthase homologues from five representative<br />
entomopathogenic fungi: Metarhizium anisopliae, Beauveria bassiana, Verticillium lecanii, Paecilomyces farinosus, and<br />
Nomuraea rileyi. Sequence comparison <strong>of</strong> the amplified <strong>of</strong> GGPP synthase homologue fragments revealed that M. anisopliae<br />
and B. bassiana have at least two different types <strong>of</strong> the GGPP synthase gene homologues. The first type (designated as ggs1),<br />
which is highly conserved among the five strains, has a unique Ser-rich region, SSXSSVSGSSS (X refers to L, A, V, or S), and is<br />
constitutively expressed throughout growth. In contrast, the second type <strong>of</strong> GGPP synthase gene homologue (ggs2) was<br />
discovered only in some strains, and genes <strong>of</strong> this type possessed high similarity to each other but showed relatively weak<br />
similarity to the ggs1 genes, with no detectable transcription under the cultivation conditions applied in this experiment. The<br />
ggs1 cloned from M. anisopliae, which encoded a putative protein <strong>of</strong> 359 amino acid residues, was heterologously expressed<br />
in E. coli. The recombinant protein showed activity to synthesize GGPP from farnesyl diphosphate and isopentenyl<br />
diphosphate. These results strongly suggested that the ggs1 gene encodes a GGPP synthase involved in primary metabolism.<br />
© 2009 Springer-Verlag.<br />
No.1267<br />
Author (s) : Singkhamanan, K., Promdonkoy, B., Chaisri, U., Boonserm, P.<br />
Title : Identification <strong>of</strong> amino acids required for receptor binding and toxicity <strong>of</strong> the Bacillus sphaericus binary toxin<br />
Source : FEMS Microbiology Letters, 303 (1), pp. 84-91.<br />
Document Type : Article<br />
Keywords : Bacillus sphaericus; Binary toxin; BinB; Mutagenesis; Receptor binding<br />
<strong>Abstract</strong> : Bacillus sphaericus produces a mosquito-larvicidal binary toxin composed <strong>of</strong> BinB and BinA subunits. BinA is important for<br />
toxicity, whereas BinB acts as a specific receptor-binding component. To study the functional significance <strong>of</strong> two regions that<br />
are only present in BinB, four block mutations and two single mutations were initially introduced: 111YLD113→ 111AAA113,<br />
115NNH117→ 115AAA117, 143GEQ145 →143AAA145, 147FQFY150→ 147AAAA150, N114A and F146A. Only the<br />
replacements at 147FQFY150 resulted in a total loss <strong>of</strong> toxicity to Culex quinquefasciatus larvae. Further single alanine<br />
substitutions in this region, F147A, Q148A, F149A and Y150A, were introduced to identify residues playing a critical role in<br />
mosquito-larvicidal activity. Larvicidal activity assays revealed that only F149A and Y150A mutants exhibited a total loss <strong>of</strong><br />
toxicity. The in vitro interaction assays demonstrated that all BinB mutants are able to interact with BinA.<br />
Immunohistochemistry analysis revealed that only the Y150A mutant was unable to bind to the larval midgut, suggesting an<br />
important role <strong>of</strong> this residue in receptor binding <strong>of</strong> the BinB subunit. Conservative aromatic substitutions at F149 and Y150<br />
resulted in full recovery <strong>of</strong> larvicidal activity, indicating that the aromaticity <strong>of</strong> F149 and Y150 is a key determinant <strong>of</strong> larvicidal<br />
activity, possibly playing a key role in the membrane interaction and receptor binding. © 2009 Federation <strong>of</strong> European<br />
Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.<br />
No.1268<br />
Author (s) : Sinsereekul, N., Wangkam, T., Thamchaipenet, A., Srikhirin, T., Eurwilaichitr, L., Champreda, V.<br />
Title : Recombinant expression <strong>of</strong> BTA hydrolase in Streptomyces rimosus and catalytic analysis on polyesters by surface plasmon<br />
resonance<br />
Source : Applied Microbiology and Biotechnology, 86 (6), pp. 1775-1784.<br />
Document Type : Article<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : Aliphatic-aromatic copolyester; Ec<strong>of</strong>lex®; Hydrolase; Streptomyces rimosus; Surface plasmon resonance; Thermobifida sp.<br />
<strong>Abstract</strong> : A recombinant polyester-degrading hydrolase from Thermobifida sp. BCC23166 targeting on aliphatic-aromatic copolyester<br />
(rTfH) was produced in Streptomyces rimosus R7. rTfH was expressed by induction with thiostrepton as a C-terminal His6<br />
fusion from the native gene sequence under the control <strong>of</strong> tipA promoter and purified from the culture supernatant to high<br />
homogeneity by a single step affinity purification on Ni-Sepharose matrix. The enzyme worked optimally at 50-55°C and<br />
showed esterase activity on C3-C16 p-nitrophenyl alkanoates with a specific activity <strong>of</strong> 76.5 U/mg on p-nitrophenyl palmitate.<br />
Study <strong>of</strong> rTfH catalysis on surface degradation <strong>of</strong> polyester films using surface plasmon resonance analysis revealed that the<br />
degradation rates were in the order <strong>of</strong> poly-ε-caprolactone >∈Ec<strong>of</strong>lex®∈>∈ polyhydroxybutyrate. Efficient hydrolysis<br />
<strong>of</strong> Ec<strong>of</strong>lex® by rTfH was observed in mild alkaline conditions, with the highest activity at pH 8.0 and ionic strength at 250 mM<br />
sodium chloride, with the maximal specific activity <strong>of</strong> 0.79 mg-1min-1mg-1 protein. Under the optimal conditions, rTfH<br />
showed a remarkable 110-time higher specific activity on Ec<strong>of</strong>lex® in comparison to a lipase from Thermomyces lanuginosus,<br />
while less difference in degradation efficiency <strong>of</strong> the two enzymes was observed on the aliphatic polyesters, suggesting<br />
greater specificities <strong>of</strong> rTfH to the aliphatic-aromatic copolyester. This study demonstrated the use <strong>of</strong> streptomycetes as an<br />
alternative expression system for production <strong>of</strong> the multi-polyester-degrading enzyme <strong>of</strong> actinomycete origin and provided<br />
insights on its catalytic properties on surface degradation contributing to further biotechnological application <strong>of</strong> this enzyme.<br />
© <strong>2010</strong> Springer-Verlag.<br />
No.1269<br />
Author (s) : Sinthupibulyakit, C., Ittarat, W., St. Clair, W.H., St. Clair, D.K.<br />
Title : p53 protects lung cancer cells against metabolic stress<br />
Source : <strong>International</strong> Journal <strong>of</strong> Oncology, 37 (6), pp. 1575-1581.<br />
Document Type : Article<br />
Keywords : 2-Deoxy-D-glucose; Cancer metabolism; Oxidative stress; p53<br />
<strong>Abstract</strong> : The preferential use <strong>of</strong> aerobic glycolysis for energy production by cancer cells, a phenomenon known as the 'Warburg<br />
effect', is well recognized and is being considered for therapeutic applications. However, whether inhibition <strong>of</strong> glycolysis will<br />
be effective in all types <strong>of</strong> cancer is unclear. The current study shows that a glycolytic inhibitor, 2-deoxy-D-glucose (2DG),<br />
exhibits the cytotoxic effect on non-small cell lung cancer in a p53-dependent manner. 2DG significantly inhibits ATP<br />
production in p53-deficient lung cancer cells (H358) but not in p53-wt cells (A549). In contrast to p53-wt cells, p53-defective<br />
cells are unable to compensate for their need <strong>of</strong> energy via oxidative phosphorylation (OXPHOS) when glycolysis is inhibited.<br />
In the presence <strong>of</strong> p53, increased ROS from OXPHOS increases the expression <strong>of</strong> p53 target genes known to modulate<br />
metabolism, including synthesis <strong>of</strong> cytochrome c oxidase 2 (SCO2) and TP53-induced glycolysis and apoptosis regulator<br />
(TIGAR). Importantly, 2DG selectively induces the expression <strong>of</strong> the antioxidant enzymes manganese superoxide dismutase<br />
(MnSOD) and glutathione peroxidase 1 (GPx1) in a p53-dependent manner. The results demonstrate that the killing <strong>of</strong> cancer<br />
cells by the inhibitor <strong>of</strong> glycolysis is more efficient in cancer cells without functional p53 and that p53 protects against<br />
metabolic stress by up-regulation <strong>of</strong> oxidative phosphorylation and modulation <strong>of</strong> antioxidants.<br />
No.1270<br />
Author (s) : Sintiprungrat, K.; Singhto, N.; Sinchaikul, S.; Chen, S.T.; Thongboonkerd, V.<br />
Title : Alterations in cellular proteome and secretome upon differentiation from monocyte to macrophage by treatment with<br />
phorbol myristate acetate: Insights into biological processes<br />
Source : JOURNAL OF PROTEOMICS, Volume: 73, Issue: 3, Special Issue: SI, Pages: 602-618, DOI: 10.1016/j.jprot.2009.08.001, Published:<br />
JAN 3 <strong>2010</strong><br />
Document Type : Article<br />
Keywords : Cellular proteins; Differentiation; Macrophage; Monocyte; Proteome; Proteomics; Secreted proteins; Secretome<br />
<strong>Abstract</strong> : Monocyte and macrophage are mainly involved in immune response and inflammatory processes. Monocytes circulate in the<br />
bloodstream and migrate to various tissues where they can differentiate to macrophages. However, the molecular basis <strong>of</strong><br />
biological processes involved in this cellular differentiation remains ambiguous. This study was to investigate alterations in<br />
cellular and secreted proteins after this differentiation phase. Macrophage was differentiated from U937 human monocytic<br />
cell line by treatment with 100 ng/ml phorbol myristate acetate (PMA) for 48 h. Cellular and secreted proteins extracted from<br />
PMA-treated cells (macrophages) were compared with those <strong>of</strong> untreated cells (monocytes) using 2-DE (n=5 gels/condition;<br />
stained with Deep Purple fluorescence dye). Quantitative intensity analysis revealed 81 and 67 protein spots whose levels<br />
were significantly altered in cellular proteome and secretome. These proteins were subsequently identified by Q-TOF MS<br />
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and/or MS/MS analyses. The altered levels <strong>of</strong> cellular elongation factor-2 (EF-2) and secreted alpha-tubulin were confirmed<br />
by Western blot analysis. Global protein network analysis demonstrated that these altered proteins were involved in cell<br />
death, lipid metabolism, cell morphology, cellular movement, and protein folding. our data may provide some insights into<br />
molecular mechanisms <strong>of</strong> biological processes upon differentiation from monocytes to macrophages. (C) 2009 Elsevier B.V. All<br />
rights reserved.<br />
No.1271<br />
Author (s) : Sirachainan, N., Sasanakul, W., Parapakpenjune, S., Wongwerawattanakoon, P., Visudibhan, A., Chuansumrit, A.<br />
Title : Protein C deficiency in Thai children with thromboembolism: A report <strong>of</strong> clinical presentations and mutation analysis<br />
Source : Thrombosis Research, 125 (2), pp. 200-202.<br />
Document Type : Letter<br />
Keywords : antigen, arginine, cysteine, fresh frozen plasma, glycine, low molecular weight heparin, nucleotide, protein<br />
C, tryptophan, warfarin; adult, amino acid analysis, amino acid substitution, anticoagulant therapy, apnea, brain edema, brain<br />
infarction, cerebral palsy, cerebrovascular accident, child, clinical article, clinical feature, conformation sensitive gel<br />
electrophoresis, deep vein thrombosis, disease course, drug dose reduction, enzyme activity, epileptic state, exon, eye<br />
examination, female, frameshift mutation, fulminating purpura, gel electrophoresis, genetic<br />
polymorphism, hemiparesis, heteroduplex analysis, human, international normalized ratio, letter, lung<br />
embolism, male, meningitis, missense mutation, mutational analysis, partial protein c deficiency, polymerase chain<br />
reaction, posterior cerebral artery, preschool child, priority journal, protein C deficiency, retina detachment, skin defect, skin<br />
necrosis, thromboembolism, vacuum assisted closure, venous thromboembolism, vitreous hemorrhage, wound care, wound<br />
healing; Asian Continental Ancestry Group, Child, Diseases in Twins, DNA Mutational Analysis, Female, Humans, Male, Protein C<br />
Deficiency, Thailand, Thromboembolism, Twins, Monozygotic<br />
<strong>Abstract</strong> : –<br />
No.1272<br />
Author (s) : Sirachainan, N.; Pakakasama, S.; Anurathapan, U.; Visudhibhan, A.; Boongird, A.; Dhanachai, M.; Larbcharoensub, N.; Hongeng, S.<br />
Title : OUTCOME OF CHILDREN WITH NEWLY OR RECURRENT DIAGNOSED HIGH GRADE CENTRAL NERVOUS SYSTEM GLIOMAS TREATED<br />
WITH CARBOPLATIN AND IRINOTECAN<br />
Source : NEURO-ONCOLOGY, Volume: 12, Issue: 6, Pages: II74-II74, Published: JUN <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1273<br />
Author (s) : Siramolpiwat, S.; Pramoolsinsap, C.; Wisedopas, N.<br />
Title : USEFULNESS OF SERUM HIGH-SENSITIVITY CRP MEASUREMENT IN DIFFERENTIATING NON-ALCOHOLIC STEATOHEPATITIS AND<br />
SIMPLE STEATOSIS IN PATIENTS WITH NON-ALCOHOLIC FATTY LIVER DISEASE<br />
Source : JOURNAL OF HEPATOLOGY, Volume: 52, Supplement: 1, Pages: S154-S154, Published: <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1274<br />
Author (s) : Siriapisith, T., Wasinrat, J., Mutirangura, P., Ruangsetakit, C., Wongwanit, C.<br />
Title : Optimization <strong>of</strong> the table speed <strong>of</strong> lower extremity CT angiography protocols in different patient age groups<br />
Source : Journal <strong>of</strong> Cardiovascular Computed Tomography, 4 (3), pp. 173-183.<br />
Document Type : Article<br />
Keywords : Cardiovascular CT; CT angiography; Lower extremity artery; Multidetector CT<br />
<strong>Abstract</strong> : Background: Scanning with 64-slice multidetector row CT (MDCT) is usually faster than blood flow in peripheral arteries <strong>of</strong> the<br />
lower extremities, and the distal arteries <strong>of</strong> lower extremities are difficult to visualize, particularly in elderly patients. Thus,<br />
the optimal table speed for CT angiography (CTA) studies should be adjusted for appropriate patient age groups. Objective:<br />
We evaluated the relative efficacy <strong>of</strong> different table speeds in several age groups <strong>of</strong> patients with suspected peripheral<br />
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arterial occlusive disease (PAOD) undergoing CTA <strong>of</strong> lower extremity arteries, as a guideline for routine use. Methods: This<br />
retrospective study reviewed routine CTA <strong>of</strong> the lower extremity arteries <strong>of</strong> 107 patients with suspected PAOD to evaluate<br />
vascular opacification in each vascular segment <strong>of</strong> 5 age groups: ≤40 years (group 1), 41-60 years (group 2), 61-70 years (group<br />
3), 71-75 years (group 4), and ≥76 years (group 5). Adequate vascular opacification was measured for attenuation in the<br />
suprarenal and infrarenal abdominal aorta and in the arteries <strong>of</strong> the lower extremity. Venous contamination was also<br />
measured. Results: Adequate vascular opacification from the suprarenal aorta to the level <strong>of</strong> the mid-popliteal artery was<br />
shown in all patients. Arterial opacification at the dorsalis pedis or plantar arteries was visualized in 85.7%-91.7% <strong>of</strong> patients<br />
and at the plantar arch arteries in 84.1%-91.7%. Minimal venous contamination was also shown adjacent to arterial<br />
enhancement, ranging from 0% to 28.6%. Conclusion: Performing CTA <strong>of</strong> the lower extremities with 64-slice MDCT could<br />
reduce the table speed to allow adequate arterial opacification and minimal venous contamination. © <strong>2010</strong> Society <strong>of</strong><br />
Cardiovascular Computed Tomography.<br />
No.1275<br />
Author (s) : Siriapisith, T., Wasinrat, J., Slisatkorn, W.<br />
Title : Computed tomography <strong>of</strong> aortic intramural hematoma and thrombosed dissection<br />
Source : Asian Cardiovascular and Thoracic Annals, 18 (5), pp. 456-463.<br />
Document Type : Article<br />
Keywords : Aneurysm; Angiocardiography; Aortic Aneurysm; Aortic Diseases; Dissecting; Tomography; X-Ray Computed<br />
<strong>Abstract</strong> : A retrospective study was undertaken to evaluate the appearance <strong>of</strong> the aortic wall on computed tomography for the<br />
purpose <strong>of</strong> developing criteria for differentiating acute aortic intramural hematoma from thrombosed false lumen seen in<br />
aortic dissection. Computed tomography angiography findings <strong>of</strong> the thoracoabdominal aorta in 23 patients with suspected<br />
intramural hematoma and 25 with thrombosed false lumen were reviewed. The more common features <strong>of</strong> an intramural<br />
hematoma were hyperattenuation <strong>of</strong> the aortic wall, wall thickness less than a quarter <strong>of</strong> the aortic diameter, intrinsic wall<br />
calcification, a lesion extending around the entire aortic circumference, and ulcer-like projections that may be precursors <strong>of</strong><br />
intramural hematoma. Wall thickness less than a quarter <strong>of</strong> the aortic diameter, lesion extending around the entire aortic<br />
circumference, and ulcer-like projections were the most useful indicators for distinguishing intramural hematoma from the<br />
thrombosed false lumen in aortic dissection. © SAGE <strong>Publications</strong> <strong>2010</strong>.<br />
No.1276<br />
Author (s) : Siriapisith, T., Wasinrat, J., Tresukosol, D.<br />
Title : Uncorrected pink tetralogy <strong>of</strong> Fallot in an adult patient: Incidental CT findings<br />
Source : Journal <strong>of</strong> Cardiovascular Computed Tomography, 4 (1), pp. 58-61.<br />
Document Type : Article<br />
Keywords : Cardiac CT; Computed tomography; Congenital heart disease; Tetralogy <strong>of</strong> Fallot<br />
<strong>Abstract</strong> : Tetralogy <strong>of</strong> Fallot (TOF), one <strong>of</strong> the most common congenital heart diseases, has four major components: right ventricular<br />
hypertrophy, overriding aorta, membranous ventricular septal defect, and right ventricular outflow tract obstruction.1 If not<br />
already present at birth, cyanosis develops in the first year <strong>of</strong> life. Survival <strong>of</strong> the patient depends on the degree <strong>of</strong><br />
pulmonary obstruction and the pulmonary blood supply. Patients rarely survive after the fourth decade <strong>of</strong> life.2-6 Limitation<br />
<strong>of</strong> blood to the lungs combined with ventricular septal defect results in supply <strong>of</strong> oxygen-poor blood to the body, causing<br />
cyanosis (blue coloration) in the patient. If the pulmonary stenosis is mild and ventricular septal defect is in balance,<br />
however, the noncyanotic patient is referred as having "pink tetralogy <strong>of</strong> Fallot.". © <strong>2010</strong> Society <strong>of</strong> Cardiovascular Computed<br />
Tomography.<br />
No.1277<br />
Author (s) : Siriarunrat, S., Lapvongwatana, P., Powwattana, A., Leerapan, P.<br />
Title : Development <strong>of</strong> a model for parent-adolescent daughter communication about sexuality<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, 41 (4), pp. 961-972.<br />
Document Type : Article<br />
Keywords : Guardian-adolescent female; Model sexuality communication tactics<br />
<strong>Abstract</strong> : This study aimed to develop a model for parent-adolescent daughter communication about sexuality (PDCS). The subjects<br />
were 18 mother-doughter pairs. The females were 12-13 years old studying in the 7th grade <strong>of</strong> a secondary school in<br />
Bangkok. The model had 3 parts: 1) the process <strong>of</strong> PDCS development, 2) the essential components, characteristics and<br />
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patterns <strong>of</strong> PDCS and 3) the impact <strong>of</strong> the program. The model was examined using both quantitative (paired ttest) and<br />
qualitative (content analysis) methods. The inputs, assessing the guardian and adolescent female factors, included sexuality<br />
knowledge, understanding <strong>of</strong> the daughter's sexual development, attitudes regarding talking about sexuality, perceptions<br />
regarding communication and the maternal-daughter relationship, and uncomfortable feelings and confidence in ability to<br />
talk about sexuality. The processes included: 1) raising awareness and enhancing positive attitudes about PDCS, 2) establishing<br />
sexual knowledge and development, 3) establishing a maternal- daughter relationship and 4) training in reciprocal PDCS skills.<br />
The output was modification <strong>of</strong> maternal and daughter communication skills that occurred during the shared discussion and<br />
reflection process. The outcomes reflected 4 aspects: feelings toward PDCS, characteristics <strong>of</strong> PDCS, sexual knowledge, and<br />
maternal-daughter relationship. The model was successful in guardian implementation and networking were established on<br />
their own with the cooperation <strong>of</strong> both subjects in accordance with their likenesses, abilities, and competencies. There was<br />
little assistance needed from the researcher or teacher to support self reliance with the PDCS.<br />
No.1278<br />
Author (s) : Siriboonpiputtana, T.; Jomsawat, U.; Rinthachai, T.; Thanakitgosate, J.; Shotivaranon, J.; Limsuwanachot, N.; Polyorat, P.;<br />
Rerkamnuaychoke, B.<br />
Title : Y-chromosomal STR haplotypes in Central Thai population<br />
Source : FORENSIC SCIENCE INTERNATIONAL-GENETICS, Volume: 4, Issue: 3, Pages: E71-E72, DOI: 10.1016/j.fsigen.2009.06.007, Published:<br />
APR <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1279<br />
Author (s) : Sirikulchayanonta, C., Iedsee, K., Shuaytong, P., Srisorrachatr, S.<br />
Title : Using food experience, multimedia and role models for promoting fruit and vegetable consumption in Bangkok kindergarten<br />
children<br />
Source : Nutrition and Dietetics, 67 (2), pp. 97-101.<br />
Document Type : Article<br />
Keywords : Food experience; Fruit and vegetable consumption; Health promotion; Multimedia; Pre-schooler; Role model<br />
<strong>Abstract</strong> : Aim: To evaluate the use <strong>of</strong> food experience, multimedia and role models for promoting fruit and vegetable consumption in<br />
kindergarten children. Methods: A quasi-experimental study was conducted. A Bangkok public primary school was randomly<br />
selected and one <strong>of</strong> the kindergarten levels was purposively chosen. Program implementation consisted <strong>of</strong> 11 activities over<br />
an eight-week period from July to September, 2003. Data on demographic variables, and types and amounts <strong>of</strong> fruit and<br />
vegetables consumed and frequency <strong>of</strong> fruit and vegetables served were collected before and after the intervention. Program<br />
evaluation consisted <strong>of</strong> an analysis <strong>of</strong> the pre- and post-test data. Results: After the intervention, fruit and vegetable eating<br />
behaviour scores (median ± interquartile range) revealed significant changes from 3 ± 8 to 7 ± 8 for vegetables and 6 ± 8 to 9<br />
± 8 for fruit (P-value < 0.001); the different types <strong>of</strong> consumed vegetables were increased from two to four (P-value ≤ 0.001);<br />
and the fruit and vegetable intake was significantly increased from 53 g to 77 g and from 11 g to 23 g respectively (P-value <<br />
0.005). Conclusions: Results <strong>of</strong> this pilot study were effective in increasing fruit and vegetable consumption in the<br />
kindergarten, however, longer-term evaluation and assessment <strong>of</strong> the impact on the home environment are required. The<br />
inclusion <strong>of</strong> nutrition education and instruction on healthy eating habits in the course curriculum combined with social<br />
support from teachers and families may improve and sustain fruit and vegetable eating behaviours. © <strong>2010</strong> The Authors.<br />
Journal compilation © <strong>2010</strong> Dietitians Association <strong>of</strong> Australia.<br />
No.1280<br />
Author (s) : Sirikulchayanonta, V., Arthit, J., Suphaneewan, J.<br />
Title : Epithelioid hemangioma involving three contiguous bones: A case report with a review <strong>of</strong> the literature<br />
Source : Korean Journal <strong>of</strong> Radiology, 11 (6), pp. 692-696.<br />
Document Type : Article<br />
Keywords : Epithelioid hemangioma spine rib imaging osseous bone<br />
<strong>Abstract</strong> : An epithelioid hemangioma involving three contiguous bones in continuity has, to the best <strong>of</strong> our knowledge, not been<br />
reported in the literature. A case <strong>of</strong> a 48- year-old man presented with radiating pain to the lower thoracic region for two<br />
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years. A radiograph and CT scan revealed both permeative osteolytic and multiple trabeculated lesions involving the left<br />
posterior part <strong>of</strong> the 10th rib as well as the 9th and 10th vertebral bodies in continuity and was misled as a malignant or<br />
infectious lesion. The histopathology and immuno-histochemistry <strong>of</strong> the lesion confirmed the diagnosis <strong>of</strong> an epithelioid<br />
hemangioma. The lesion was still stableas <strong>of</strong> three years after surgery.<br />
No.1281<br />
Author (s) : Siriphorn, A., Chompoopong, S., Floyd, C.L.<br />
Title : 17β-Estradiol protects Schwann cells against H2O2-induced cytotoxicity and increases transplanted Schwann cell survival in a<br />
cervical hemicontusion spinal cord injury model<br />
Source : Journal <strong>of</strong> Neurochemistry, 115 (4), pp. 864-872.<br />
Document Type : Article<br />
Keywords : Cell transplantation; Cervical; Estrogen; Oxidative injury; Schwann cell; Spinal cord injury<br />
<strong>Abstract</strong> : Schwann cell (SC) transplantation is a promising repair strategy after spinal cord injury (SCI); however, a large number <strong>of</strong> SCs<br />
do not survive following transplantation. Previous studies have shown that 17β-estradiol (E2) protects several cell types<br />
against cytotoxicity. Thus, this study evaluated the protective potential <strong>of</strong> E2 on SCs in vitro and investigated the effect <strong>of</strong> E2<br />
on transplanted SC survival in a rat model <strong>of</strong> SCI. Primary SC cultures were found to robustly express estrogen receptors (ER)<br />
and incubation with E2 protected SCs against hydrogen peroxide-induced cell death. This protection was not inhibited by the<br />
ER antagonist ICI 182,780, suggesting that genomic signaling is not necessary for protection. In a subsequent experiment,<br />
cervical hemicontusion SCI was induced in male rats followed by sustained administration <strong>of</strong> E2 or placebo. Eight days after<br />
SCI, SCs were transplanted into the injury epicenter. E2 treatment significantly increased the number <strong>of</strong> surviving labeled<br />
transplanted SCs evaluated 7 days after transplantation. These data demonstrate that E2 protects SCs against oxidative stress<br />
and improves transplanted SC survival, which suggests that E2 administration may be an intervention <strong>of</strong> choice for enhancing<br />
survival <strong>of</strong> transplanted SCs after SCI. © <strong>2010</strong> The Authors. Journal Compilation © <strong>2010</strong> <strong>International</strong> Society for<br />
Neurochemistry.<br />
No.1282<br />
Author (s) : Siriphukpong, S., Pattanaprichakul, P., Sitthinamsuwan, P., Karoopongse, E.<br />
Title : Granulomatous mycosis fungoides with large cell transformation misdiagnosed as leprosy<br />
Source : ournal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, 93 (11), pp. 1321-1326.<br />
Document Type : Article<br />
Keywords : Granuloma annulare-like; Granulomatous mycosis fungoides; Large cell transformation; Leprosy<br />
<strong>Abstract</strong> : Background: Granulomatous mycosis fungoides is an unusual histopathological variant <strong>of</strong> cutaneous T-cell lymphoma without<br />
clinical distinction from classic mycosis fungoides. Symptoms associated with peripheral nerve involvement have rarely been<br />
reported in the literature. Case Report: The authors described a case <strong>of</strong> granulomatous MF stage IIB with large cell<br />
transformation who initially presented with leprosy-like condition and chronic left peroneal neuropathy. The patient received<br />
six courses <strong>of</strong> gemcitabine with greater than 90% improvement <strong>of</strong> skin lesions. The rest <strong>of</strong> the lesions were successfully<br />
treated with local electron beam radiation. Conclusion: Granulomatous MF with neuropathy can be clinically misdiagnosed if<br />
there is no histopathological and immunohistochemical finding to support the diagnosis <strong>of</strong> lymphoma.<br />
No.1283<br />
Author (s) : Sirisali, K., Manochiopinij, S., Leelahakul, P., Ruengrai, V., Sattayakom, A., Sirisali, S.<br />
Title : Critical value <strong>of</strong> the clinical laboratory test in Thailand.<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand = Chotmaihet thangphaet, 93 Suppl 6, pp. S22-27.<br />
Document Type : Article<br />
Keywords : article, clinical chemistry, diagnostic procedures, health care quality, hospital, hospital laboratory, human, practice<br />
guideline, questionnaire, standard, Thailand; Chemistry, Clinical, Clinical Laboratory<br />
Techniques, Hospitals, Humans, Laboratories, Hospital, Practice Guidelines as Topic, Quality Assurance, Health<br />
Care, Questionnaires, Reference Standards, Thailand<br />
<strong>Abstract</strong> : The critical values are the values <strong>of</strong> laboratory testing results which required attention or action by the physicians. It is an<br />
essential component <strong>of</strong> good laboratory practice and widely used throughout the world. The present study examined the<br />
current situation on implementing <strong>of</strong> critical value list (CVL) <strong>of</strong> Thai clinical laboratory and what factors were involved in their<br />
consideration. A questionnaire composed <strong>of</strong> 3 main categories made up <strong>of</strong> 34 questions was mailed to 450 Thai clinical<br />
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laboratories. These participated laboratories were randomly selected from both private-and government-hospitals.<br />
Participated ones were requested to answer the questionnaire and return via mail within two months. Data were analyzed by<br />
Chi-square test on Micros<strong>of</strong>t Excel. The results showed that there were only 48.9% <strong>of</strong> Thai laboratories implemented the CVL.<br />
It was found that there were many factors which governed the implementation <strong>of</strong> critical values. These factors were<br />
significantly different between those who implement the critical values and those did not (p < 0.01). In regard to private- and<br />
government-hospital laboratories, implementation <strong>of</strong> CVL was not significant difference (p > 0.1). However, it was found that<br />
assigned persons who responded to notify and act on the critical value was significantly different (p < 0.01). Moreover there<br />
were no significant differences on laboratory policy, communication method as well as standard operating procedures on<br />
critical values between the private- and government-hospital laboratories (p > 0.1). There were only 20.2% <strong>of</strong> those who<br />
implemented the CVL and considered this action as a non-troublesome matter. But, a large group <strong>of</strong> 95.7% considered this<br />
matter as an extra-ordinary tool for quality control <strong>of</strong> result reporting system. Thai laboratories perceived the implementation<br />
<strong>of</strong> critical values list differently. There were some factors beyond their consideration. However utilizing <strong>of</strong> CVL would be an<br />
extra-ordinary tool for assuring test results.<br />
No.1284<br />
Author (s) : Sirisali K., Manochiopinij S., Leelahakul P., Vattanaviboon P., Wonglumsom W., Sirisali S.<br />
Title : A smart model for clinical laboratory personnel development.<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand = Chotmaihet thangphaet, Volume 93 Suppl 6, November <strong>2010</strong>, Pages S203-<br />
207<br />
Document Type : Article<br />
Keywords : article, education, evaluation, human, laboratory, laboratory personnel, organization and management, personnel<br />
management, pilot study, pr<strong>of</strong>essional competence, program development, quality control, research, standard, Thailand;<br />
Humans, Laboratories, Medical Laboratory Personnel, Pilot Projects, Pr<strong>of</strong>essional Competence, Program Development, Quality<br />
Control, Research, Staff Development, Thailand<br />
<strong>Abstract</strong> : To become a quality clinical laboratory, personnel development is the most important factor. In order to achieve this goal, it<br />
should emphasize that clinical laboratory is not only a testing laboratory; it must be a knowledge-based service laboratory. A<br />
smart model for clinical laboratory personnel development under the Human Asset Development (HAD) program had been<br />
launched since 2003. To strengthen the competency <strong>of</strong> clinical laboratory personnel, an appropriate model was developed<br />
and apply to the clinical laboratory personnel. Medical technologist who currently worked in clinical laboratory participated<br />
in this study. The proposed model consisted <strong>of</strong> 3 phases. 1) The knowledge providing via update and refresher courses. 2)<br />
Application <strong>of</strong> learned knowledge to practice under close supervision. 3) Training on special topic and self oriented research<br />
activity. The outcome <strong>of</strong> 5 years project was evaluated. After the first phase, they were able to identify and solve their own<br />
troublesome under ours close supervision. There were 25 projects presented within 3 years. The last phase, they were very<br />
constructive. Nine projects <strong>of</strong> self created had been presented. Those projects contained clear objectives and were able to<br />
implement. The smart model for clinical laboratory personnel development leaded to many self created projects in a few<br />
years. Thus, this implies its important role in human resource development that should be continued. The keys index <strong>of</strong><br />
success were ours strong intention, with providing motivation and periodically encouragement to the participants, and keep<br />
going on consistently.<br />
No.1285<br />
Author (s) : Sirisinha, K., Boonkongkaew, M., Kositchaiyong, S.<br />
Title : The effect <strong>of</strong> silane carriers on silane grafting <strong>of</strong> high-density polyethylene and properties <strong>of</strong> crosslinked products<br />
Source : Polymer Testing, 29 (8), pp. 958-965.<br />
Document Type : Article<br />
Keywords : Crosslinking; Grafting; High-density polyethylene; Silane<br />
<strong>Abstract</strong> : High-density polyethylene (HDPE) was melt-grafted with vinyl trimethoxysilane using dicumyl peroxide as initiator. Three types<br />
<strong>of</strong> ethylene copolymers were used as silane carriers, and were evaluated for their ability to assist the silane-grafting and<br />
water-crosslinking <strong>of</strong> HDPE. FTIR revealed that an increase in the amount <strong>of</strong> silane absorbed in the carriers did not help<br />
improve the silane-grafting efficiency. However, the gel and rheological results showed that those carriers promoted the rate<br />
<strong>of</strong> crosslinking and increased the density <strong>of</strong> the crosslink network. Ethylene vinyl acetate showed a stronger effect in this<br />
respect than ethylene octene and ethylene-butene copolymers. Analysis <strong>of</strong> the crosslinked materials by heat distortion<br />
temperature (HDT) test and thermogravimetric analysis (TGA) indicated a marked increase in distortion and decomposition<br />
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temperatures, as well as thermal activation energy, compared to the neat HDPE. The presence <strong>of</strong> silane crosslink also<br />
resulted in a slight improvement in tensile yield stress and modulus. © <strong>2010</strong> Elsevier Ltd. All rights reserved.<br />
No.1286<br />
Author (s) : Siriswangvat, S., Sansanayudh, N., Nathisuwan, S., Panomvana, D.<br />
Title : Comparison between the effect <strong>of</strong> omeprazole and rabeprazole on the antiplatelet action <strong>of</strong> clopidogrel<br />
Source : Circulation Journal, 74 (10), pp. 2187-2192.<br />
Document Type : Article<br />
Keywords : Clopidogrel; Omeprazole; Platelet aggregation; Rabeprazole<br />
<strong>Abstract</strong> : Background: Emerging data suggests that several proton pump inhibitors (PPIs), including omeprazole, might interfere with the<br />
antiplatelet action <strong>of</strong> clopidogrel. However, there is a lack <strong>of</strong> data for rabeprazole. This study aimed to investigate and<br />
compare the impact <strong>of</strong> omeprazole and rabeprazole on the antiplatelet action <strong>of</strong> clopidogrel among patients with coronary<br />
artery disease (CAD). Methods and Results: A prospective, randomized, open-labeled study was conducted among 87 CAD<br />
patients receiving clopidogrel and aspirin. Forty three and 44 patients were randomized to receive omeprazole 20 mg and<br />
rabeprazole 20 mg once daily, respectively, for at least 2 weeks. Adenosine 5-diphosphate 20 μmol/L-induced platelet<br />
aggregation was performed before and after PPIs treatment. Mean maximal platelet aggregation (MPA) before and after PPIs<br />
treatment <strong>of</strong> both groups were compared. At baseline, there were no significant differences in the mean MPA between the<br />
omeprazole and rabeprazole groups (40.68±18.82% vs 36.42±21.39%; P=0.326). After a 2-week treatment with PPIs, the mean<br />
MPA in both groups significantly increased from baseline and there were no differences between the omeprazole and<br />
rabeprazole groups (55.73±19.66% vs 48.46±18.80%; P=0.141). Conclusions: Both omeprazole and rabeprazole decreased the<br />
antiplatelet effect <strong>of</strong> clopidogrel. Use <strong>of</strong> these agents resulted in a similar degree <strong>of</strong> interference on clopidogrel's action, as<br />
measured by ADP-induced platelet aggregation.<br />
No.1287<br />
Author (s) : Siritho, S., Prayoonwiwat, N., Pulkes, T., Chankrachang, S., Laptikultham, S., Nidhinandana, S., Sethawacharawanit, S.,<br />
Tantirittisak, T., Arayawichanont, A.<br />
Title : A retrospective study <strong>of</strong> multiple sclerosis in Thailand<br />
Source : Neurology Asia, 15 (3), pp. 253-261.<br />
Document Type : Article<br />
Keywords : adult, article, cerebrospinal fluid analysis, clinical feature, controlled study, demography, demyelinating disease, disease<br />
association, disease course, disease duration, Expanded Disability Status Scale, family history, female, human, major clinical<br />
study, male, multiple sclerosis, neuroimaging, onset age, prevalence, recurrence risk, retrospective study, sex<br />
ratio, symptomatology, Thailand, visual impairment<br />
<strong>Abstract</strong> : Objectives: To determine the prevalence <strong>of</strong> Thai demyelinating diseases regarding demographic data, symptoms and signs,<br />
associated diseases, disease progression, cerebrospinal fluid analysis and imaging findings. Methods: A multicenter<br />
retrospective study <strong>of</strong> 107 MS patients attending the Neurological Centers in Thailand during June and December 2004 was<br />
performed. Each had an initial diagnosis <strong>of</strong> demyelinating diseases. Results: From 107 patients, there were 78.5% female and<br />
21.5% male with the female: male ratio <strong>of</strong> 3.7:1. The age at onset was 32.7±11.5 years. The mean disease duration was<br />
3.8±5.1 years and the mean number <strong>of</strong> relapses was 4.6±4.4 with annual relapse rate <strong>of</strong> 1.5±1.3 times. None reported a<br />
family history <strong>of</strong> MS. Recurrent optico-spinal form was 27.1% followed by 17.8% <strong>of</strong> spinal form and 15% <strong>of</strong> western form <strong>of</strong><br />
MS. The most common presenting symptom was visual impairment (51.4%). Only 24.1% demonstrated oligoclonal bands in<br />
CSF. The median score <strong>of</strong> EDSS at their latest visits was 3.0 with mean score <strong>of</strong> 3.8±3.0. Conclusions: MS in Thailand is<br />
different from Western countries. There were no occurrence <strong>of</strong> MS in families, higher incidence <strong>of</strong> visual impairment at onset,<br />
more common recurrent optico-spinal form and lower incidence <strong>of</strong> oligoclonal bands in the CSF.<br />
No.1288<br />
Author (s) : Siritho, S.; Nakashima, I.; Takahashi, T.; Fujihara, K.; Prayoonwiwat, N.<br />
Title : Anti-AQP4 antibody status in Thai demyelinating diseases: A Thai-Japanese collaborative study<br />
Source : MULTIPLE SCLEROSIS, Volume: 16, Issue: 10, Pages: 1289-1289, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.1289<br />
Author (s) : Siriussawakul, A., Mandee, S., Thonsontia, J., Vitayaburananont, P., Areewatana, S., Laonarinthawoot, J.<br />
Title : Obesity, epidural analgesia, and subcostal incision are risk factors for postoperative desaturation<br />
Source : Canadian Journal <strong>of</strong> Anesthesia, 57 (5), pp. 415-422.<br />
Document Type : Article<br />
Keywords : bupivacaine, fentanyl, morphine; abdominal surgery, adult, age, arterial oxygen desaturation, arterial oxygen<br />
saturation, article, bleeding, continuous infusion, descriptive research, drug dose titration, epidural<br />
anesthesia, female, human, hypoxemia, incision, intraoperative period, major clinical study, male, night, obesity, operation<br />
duration, oxygenation, postoperative analgesia, postoperative complication, priority journal, risk factor, smoking, subcostal<br />
incision; Abdomen, Adult, Age Factors, Aged, Analgesia, Epidural, Analgesics, Opioid, Blood Loss, Surgical, Female, Hospitals,<br />
<strong>University</strong>, Humans, Incidence, Male, Middle Aged, Multivariate Analysis, Obesity, Oxygen, Postoperative<br />
Complications, Prospective Studies, Risk Factors, Smoking, Time Factors<br />
<strong>Abstract</strong> : Purpose: This study was designed to determine the incidence <strong>of</strong> oxygen desaturation after upper abdominal surgery during<br />
the first 48 hr on general surgical wards and also to identify risk factors for oxygen desaturation. Methods: This descriptive<br />
study was conducted in 206 patients not expected to receive supplemental oxygen postoperatively who were undergoing<br />
upper abdominal surgery in a tertiary care university hospital. Desaturation was classified either as constant, i.e., oxygen<br />
saturation < 90% for > three minutes or < 85% once or as episodic, i.e., when oxygen saturation is decreased by ≥ 5%<br />
below baseline for one to two minutes ten times or more during the night period. Possible risk factors were elderly patients<br />
(> 70 yr), obesity (body mass index [BMI] > 25 kg.m-2), smoking, surgical time > 180 min, postoperative pain control<br />
methods, intraoperative blood loss, and site <strong>of</strong> incision. Results: Of the 206 patients enrolled, 171 were retained for analysis.<br />
Desaturation occurred in 65 patients (38%). Forty-eight <strong>of</strong> these had constant hypoxemia with nadir oxygen saturation values<br />
ranging from 71-89%. The remaining 17 patients experienced nocturnal episodic hypoxemia. After multivariate analysis, the<br />
three factors that correlated with postoperative desaturation were BMI > 25 (adjusted odds ratio [OR] 3.06; 95% confidence<br />
interval (CI) 1.38-6.79; P = 0.006), subcostal incision (OR 2.68; 95%CI 1.34-5.38; P = 0.005), and neuraxial opioids (OR 2.44;<br />
95%CI 1.21-4.91; P = 0.013). Conclusion: Oxygen desaturation is common after upper abdominal surgery, and the risk factors<br />
are obesity, a subcostal incision, and neuraxial opioid administration. © <strong>2010</strong> Canadian Anesthesiologists' Society.<br />
No.1290<br />
Author (s) : Sirovetnukul, R., Chutima, P.<br />
Title : Multi-objective particle swarm optimization with negative knowledge for U-shaped assembly line worker allocation problems<br />
Source : IEEM<strong>2010</strong> - IEEE <strong>International</strong> Conference on Industrial Engineering and Engineering Management, art. no. 5674252, pp. 2033-<br />
2038.<br />
Document Type : Conference Paper<br />
Keywords : Multiple objectives; Negative knowledge; Particle swarm optimization; U-shaped assembly lines; Worker allocation<br />
<strong>Abstract</strong> : A Single U-shaped Assembly Line (SUAL) is a type <strong>of</strong> Just-In-Time (JIT) production system where a variety <strong>of</strong> product models<br />
with similar product characteristics are assembled. Worker allocation to the SUAL is crucial to achieve the main benefits <strong>of</strong> JIT<br />
with the minimum <strong>of</strong> number <strong>of</strong> workers, equity <strong>of</strong> workload and the shortest walking time. A novel algorithm, named<br />
Particle Swarm Optimization with Negative Knowledge (PSONK), is proposed to find the Pareto-optimal solutions for SUAL<br />
worker allocation problems with from seven to two hundred and ninety-seven tasks. The performance <strong>of</strong> PSONK are<br />
compared with Non-dominated Sorting Genetic Algorithm-II (NSGA-II) against the measures <strong>of</strong> convergence, spread, ratio <strong>of</strong><br />
Pareto-optimal solutions, and CPU time. PSONK outperforms NSGA-II for most performance measures. ©<strong>2010</strong> IEEE.<br />
No.1291<br />
Author (s) : Sithisarn, P.; Jarikasem, S.; Thisayakorn, K.; Supatanakul, W.; Arunpairojana, V.<br />
Title : Adaptogenic-related activity and phenolic content <strong>of</strong> selected ginseng-like herbs in Thailand<br />
Source : PLANTA MEDICA, Volume: 76, Issue: 12, Pages: 1189-1190, Published: AUG <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1292<br />
Author (s) : Sitthidet, C., Stevens, J.M., Field, T.R., Layton, A.N., Korbsrisate, S., Stevens, M.P.<br />
469
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Actin-based motility <strong>of</strong> Burkholderia thailandensis requires a central acidic domain <strong>of</strong> BimA that recruits and activates the<br />
cellular Arp2/3 complex<br />
Source : Journal <strong>of</strong> Bacteriology, 192 (19), pp. 5249-5252.<br />
Document Type : Article<br />
Keywords : actin, actin related protein 2, actin related protein 3, bacterial protein, protein BimA, unclassified drug, actin binding protein;<br />
actin polymerization, animal cell, article, bacterium mutant, Burkholderia, Burkholderia infection, Burkholderia<br />
thailandensis, mouse, nonhuman, priority journal, protein binding, protein domain, confocal<br />
microscopy, genetics, metabolism, polyacrylamide gel electrophoresis; Burkholderia, Burkholderia thailandensis;<br />
Actins, Bacterial Proteins, Burkholderia, Electrophoresis, Polyacrylamide Gel, Micr<strong>of</strong>ilament Proteins, Microscopy, Confocal<br />
<strong>Abstract</strong> : Burkholderia species use BimA for intracellular actin-based motility. Uniquely, Burkholderia thailandensis BimA harbors a<br />
central and acidic (CA) domain. The CA domain was required for actin-based motility, binding to the cellular Arp2/3 complex,<br />
and Arp2/3-dependent polymerization <strong>of</strong> actin monomers. Our data reveal distinct strategies for actin-based motility among<br />
Burkholderia species. Copyright © <strong>2010</strong>, American Society for Microbiology. All Rights Reserved.<br />
No.1293<br />
Author (s) : Sitthinamsuwan, B., Chanvanitkulchai, K., Nunta-Aree, S., Kumthornthip, W., Pisarnpong, A., Ploypetch, T.<br />
Title : Combined ablative neurosurgical procedures in a patient with mixed spastic and dystonic cerebral palsy<br />
Source : Stereotactic and Functional Neurosurgery, 88 (3), pp. 187-192.<br />
Document Type : Article<br />
Keywords : Cerebral palsy; Cervical dystonia; Microsurgical dorsal root entry zone lesion; Selective dorsal rhizotomy; Selective<br />
peripheral denervation; Selective peripheral neurotomy; Spasticity<br />
<strong>Abstract</strong> : Background: Harmful generalized spasticity is an obstacle in rehabilitation and caregiving. Neurosurgical intervention is a<br />
therapeutic option for patients with severe spasticity who do not respond to nonoperative management. Currently,<br />
intrathecal bacl<strong>of</strong>en therapy (ITB) is a good alternative treatment for such patients. However, the ITB device is costly and the<br />
intrathecal drug is not available in Thailand. Case Description: We report a combined use <strong>of</strong> ablative neurosurgical procedures<br />
in a patient with severe generalized spasticity and disabling cervical dystonia (CD). The assembled operations including<br />
selective peripheral denervation for CD, microsurgical dorsal root entry zone lesion for upper limb spasticity, and selective<br />
dorsal rhizotomy for lower limb spasticity were conducted in a single session. Furthermore, recurrent spasticity <strong>of</strong> the upper<br />
extremities was subsequently treated by selective peripheral neurotomy. Results: The spasticity and CD totally disappeared<br />
after all operations. The patient became able to sit and perform head turning. Additionally, we also found an improvement in<br />
swallowing and the voluntary movement <strong>of</strong> the lower limbs. © <strong>2010</strong> S. Karger AG, Basel.<br />
No.1294<br />
Author (s) : Sitthinamsuwan, P., Pongpruttipan, T., Chularojmontri, L., Pattanaprichakul, P., Khuhapinant, A., Sukpanichnant, S.<br />
Title : Extranodal NK/T cell lymphoma, nasal type, presenting with primary Cutaneous lesion mimicking Granulomatous Panniculitis:<br />
A case report and review <strong>of</strong> literature<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, 93 (8), pp. 1001-1007.<br />
Document Type : Article<br />
Keywords : Cutaneous lymphoma; Epstein barr virus; Extranodal NK/T-cell lymphoma<br />
<strong>Abstract</strong> : Background: Cutaneous extranodal NK/T-cell lymphoma, nasal type (NK/T) is relatively rare, associated with aggressive<br />
behavior and poor prognosis. Histopathological findings, immunohistochemical study, and EBV-encoded RNA (EBER) in situ<br />
hybridization are essential for the diagnosis. Case Report: A 54-year-old Thai man with NK/T <strong>of</strong> the nasal cavity initially<br />
presented with cutaneous NK/T mimicking granulomatous panniculitis. The skin biopsies were performed twice due to the<br />
marked necrosis in the first one. The second biopsy revealed small, medium, and large atypical lymphoid cells infiltrating fat<br />
lobules with necrotic foci and granulomatous reaction. Within the ranulomatous inflammation, the atypical lymphoid cells<br />
showing involvement <strong>of</strong> the blood vessel (angiocentricity) were noted. Immunostaining demonstrated that the atypical<br />
lymphoid cells marked with CD3, CD56, and TIA-1, but they did not mark with CD5, CD20, CD15, or CD 30. EBER in situ<br />
hybridization was positive. Conclusion: Cutaneous NK/T can produce granulomatous panniculitis. The recognition <strong>of</strong> atypical<br />
lymphoid cells showing angiocentricity together with immunohistochemistry and EBER in situ hybridization are crucial for the<br />
correct diagnosis.<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.1295<br />
Author (s) : Sitthinamsuwan, P.<br />
Title : Cutaneous involvement by extranodal NK/T-cell lymphoma <strong>of</strong> the colon, histologically mimicking mycosis fungoides: A case<br />
retort<br />
Source : JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY, Volume: 62, Issue: 3, Supplement: S, Pages: AB97-AB97, Published:<br />
MAR <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1296<br />
Author (s) : Sittishoktram, M., Asawapirom, U., Osotchan, T.<br />
Title : Optical and electrical properties <strong>of</strong> modified polythiophene with tetrafluorobenzene thin film<br />
Source : Advanced Materials Research, 93-94, pp. 574-577.<br />
Document Type : Conference Paper<br />
Keywords : Modified poly(3-hexylthiophene); Optical properties; Time <strong>of</strong> flight measurement<br />
<strong>Abstract</strong> : Modified poly(3-hexylthiophene) (P3HT) with molecule <strong>of</strong> 1,4-dithienyl-2,3,5,6-tetrafluorobenzene in the main chain was<br />
developed as a new class <strong>of</strong> conjugated polymer with high stability. The structural and optical characteristics <strong>of</strong> modified<br />
polymer were investigated by x-ray diffraction (XRD) and optical absorption spectroscopy. By comparing to characteristic <strong>of</strong><br />
P3HT, the XRD pattern <strong>of</strong> modified polymer showed the diffraction peak shifted from theta <strong>of</strong> 5.4° to 5.9° with decrease<br />
intensity. This indicates that the modified polymer chain has a reducing in structural coplanarity and crystallinity. Since the<br />
electrical property can be related to the morphology and structure <strong>of</strong> thin film, the electrical conduction <strong>of</strong> modified<br />
polymer was studied with the structure <strong>of</strong> ITO/modified P3HT/Al. The result <strong>of</strong> I-V characteristic measurement <strong>of</strong> modified<br />
polymer as a function <strong>of</strong> temperatures showed that the conductance decreased with decreasing temperature. The mobility <strong>of</strong><br />
copolymer was also evaluated by using time <strong>of</strong> flight measurement and mobility value <strong>of</strong> 5x10-4 cm2/Vs was obtained at<br />
room temperature. © (<strong>2010</strong>) Trans Tech <strong>Publications</strong>.<br />
No.1297<br />
Author (s) : Sittishoktram, M.; Asawapirom, U.; Osotchan, T.<br />
Title : Relationship between electrical properties and structure for modified poly(3-hexylthiophene) copolymer thin films<br />
Source : INEC <strong>2010</strong> - <strong>2010</strong> 3rd <strong>International</strong> Nanoelectronics Conference, Proceedings, <strong>2010</strong>, Article number 5424648, Pages 226-227<br />
Document Type : Conference Paper<br />
Keywords : Acceptor molecules, Aluminum electrodes, Benzothiadiazoles, Carrier conduction, Coplanarity, Copolymer thin films,<br />
Crystallinities, Diffraction peaks, Electrical property, IV characteristics, Main chains, Modified polymer, Molecular<br />
ordering, Optical characteristics, Poly-3-hexylthiophene, Room temperature, Time <strong>of</strong> flight measurements, UV-visible<br />
absorption spectroscopy, XRD patterns; Absorption spectroscopy, Benzene, Carrier mobility, Nanoelectronics, Plastic<br />
films, Polymeric films, Polymers, Thin films, X ray diffraction, X ray diffraction analysis; Electric properties<br />
<strong>Abstract</strong> : Relationship between the changing in structure and the electrical properties <strong>of</strong> modified poly(3-hexylthiophene) (P3HT) was<br />
examined. For modified P3HT two types <strong>of</strong> acceptor molecules including 1,4-Bis(5-brothien-2-yl)-2,3,5,6- tetrafluoro benzene<br />
(TFT) and 2,1,3-benzothiadiazole (Bz) were added into the main chain <strong>of</strong> P3HT. These modifications have the effect to<br />
molecular ordering <strong>of</strong> modified polymer thin film. The structural and optical characteristics <strong>of</strong> modified polymer were<br />
investigated by X-ray diffraction (XRD) and UV-visible absorption spectroscopy. By comparing to characteristic <strong>of</strong> pristine P3HT,<br />
the XRD pattern <strong>of</strong> modified polymer showed the shift <strong>of</strong> diffraction peak with decrease in intensity. These indicate that<br />
modified polymer chain reduced in structural coplanarity and crystallinity. Since the electrical property relates to the<br />
morphology and structure <strong>of</strong> thin film, the I-V characteristic <strong>of</strong> modified polymer thin film was measured with aluminum<br />
electrode and the results show the decrease in current relative to that in pristine P3HT. Furthermore, the mobility <strong>of</strong><br />
modified polymer was also evaluated by using time <strong>of</strong> flight measurement with the structure <strong>of</strong> ITO/modified P3HT/Al at<br />
room temperature. The measured mobilities <strong>of</strong> modified is agree well with the I-V characteristics and this can demonstrate<br />
the effect <strong>of</strong> molecular ordering on its carrier conduction. ©<strong>2010</strong> IEEE.<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.1298<br />
Author (s) : Sivilaikul, S., Jitprom, A., Kularb, A., Kornkaewrut, K., Suthanmaphinuth, P., Mahasawangkul, S., Saikhun, K., Wajjwalku, W.,<br />
Thongtipsiridech, S.<br />
Title : Relationship between seminal and serum calcium concentration with semen quality in the Asian elephant (Elephas maximus)<br />
Source : Thai Journal <strong>of</strong> Veterinary Medicine, 40 (3), pp. 251-255.<br />
Document Type : Article<br />
Keywords : Asian elephant (Elephas maximus); Calcium; Semen quality<br />
<strong>Abstract</strong> : The purpose <strong>of</strong> this study is to identify the relationship <strong>of</strong> calcium concentration in seminal plasma on elephant semen<br />
quality including <strong>of</strong> volume, concentration, pH and percentage <strong>of</strong> progressive motility, dead sperm and abnormal<br />
morphology, respectively. Semen collection and evaluation were done in 9 elephants <strong>of</strong> Thai Elephant Conservation Centre,<br />
National Elephant Institute, Forest Industry Organization at Lampang. Calcium in seminal plasma was measured by using<br />
colorimetric method. Data were analyzed by using Linear Regression. The results revealed that amount <strong>of</strong> calcium in seminal<br />
plasma was negative correlated with only percentage <strong>of</strong> progressive motility (p5-40% and<br />
>50%, respectively) and analyzed by using Repeated measure ANOVA. The results revealed that percentage <strong>of</strong> dead sperm<br />
and abnormal morphology and concentration <strong>of</strong> calcium in seminal plasma <strong>of</strong> all groups were significantly difference<br />
(p
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.1300<br />
Author (s) : Smithuis, F., Kyaw, M.K., Phe, O., Win, T., Aung, P.P., Oo, A.P.P., Naing, A.L., Nyo, M.Y., Myint, N.Z.H., Imwong, M., Ashley, E., Lee,<br />
S.J., White, N.J.<br />
Title : Effectiveness <strong>of</strong> five artemisinin combination regimens with or without primaquine in uncomplicated falciparum malaria: An<br />
open-label randomised trial<br />
Source : The Lancet Infectious Diseases, 10 (10), pp. 673-681.<br />
Document Type : Article<br />
Keywords : amodiaquine plus artesunate, artemether plus benflumetol, artesunate, artesunate plus mefloquine, dihydroartemisinin plus<br />
piperaquine, mefloquine, primaquine; abdominal pain, adolescent, anorexia, article, child, clinical trial, combination<br />
chemotherapy, controlled clinical trial, controlled study, diarrhea, dizziness, drug efficacy, female, follow up, headache, heart<br />
palpitation, human, infant, insomnia, major clinical study, malaria falciparum, male, mixed infection, multicenter<br />
study, nausea, Plasmodium falciparum, preschool child, priority journal, randomized controlled trial, reinfection, school<br />
child, single drug dose, treatment outcome, vomiting; Adolescent, Adult, Aged, Aged, 80 and<br />
over, Antimalarials, Artemisinins, Child, Child, Preschool, Drug Therapy, Combination, Female, Humans, Infant, Malaria,<br />
Falciparum, Malaria, Vivax, Male, Mefloquine, Middle Aged, Myanmar, Plasmodium falciparum, Primaquine, Treatment<br />
Outcome, Young Adult<br />
<strong>Abstract</strong> : Background: Artemisinin-combination therapy (ACT) is recommended as first-line treatment <strong>of</strong> falciparum malaria throughout<br />
the world, and fixed-dose combinations are preferred by WHO; whether a single gametocytocidal dose <strong>of</strong> primaquine should<br />
be added is unknown. We aimed to compare effectiveness <strong>of</strong> four fixed-dose ACTs and a loose tablet combination <strong>of</strong><br />
artesunate and mefloquine, and assess the addition <strong>of</strong> a single gametocytocidal dose <strong>of</strong> primaquine. Methods: In an openlabel<br />
randomised trial in clinics in Rakhine state, Kachin state, and Shan state in Myanmar (Burma) between Dec 30, 2008, and<br />
March 20, 2009, we compared the effectiveness <strong>of</strong> all four WHO-recommended fixed-dose ACTs (artesunate-mefloquine,<br />
artesunate-amodiaquine, dihydroartemisinin-piperaquine, artemether-lumefantrine) and loose artesunate-mefloquine in<br />
Burmese adults and children. Eligible patients were those who presented to the clinics with acute uncomplicated<br />
Plasmodium falciparum malaria or mixed infection, who were older than 6 months, and who weighed more than 5 kg.<br />
Treatments were randomised in equal numbers within blocks <strong>of</strong> 50 and allocation was in sealed envelopes. All patients were<br />
also randomly assigned to receive either a single dose <strong>of</strong> primaquine 0·75 mg base/kg or not. Patients were followed up for<br />
63 days. Treatment groups were compared by analysis <strong>of</strong> variance and multiple logistic regression. The primary outcome was<br />
the 63 day recrudescence rate. This study is registered with clinicaltrials.gov, number NCT00902811. Findings: 155 patients<br />
received artesunate-amodiaquine, 162 artemether-lumefantrine, 169 artesunate-mefloquine, 161 loose artesunatemefloquine,<br />
and 161 dihydroartemisinin-piperaquine. By day 63 <strong>of</strong> follow-up, 14 patients (9·4%; 95% CI 5·7-15·3%) on<br />
artesunate-amodiaquine had recrudescent P falciparum infections, a rate significantly higher than for artemether-lumefantrine<br />
(two patients; 1·4%; 0·3-5·3; p=0·0013), fixed-dose artesunate-mefloquine (0 patients; 0-2·3; p
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Source : Cellular and Molecular Life Sciences, 67 (19), pp. 3371-3387.<br />
Document Type : Article<br />
Keywords : Atypical chemokine receptor; Cancer; Duffy antigen receptor for<br />
chemokines; HIV; Immunoaffinity; Inflammation; Plasmodium vivax; VHH<br />
<strong>Abstract</strong> : Fy blood group antigens are carried by the Duffy antigen receptor for chemokines (DARC), a red cells receptor for Plasmodium<br />
vivax broadly implicated in human health and diseases. Recombinant VHHs, or nanobodies, the smallest intact antigen<br />
binding fragment derivative from the heavy chain-only antibodies present in camelids, were prepared from a dromedary<br />
immunized against DARC N-terminal extracellular domain and selected for DARC binding. A described VHH, CA52, does<br />
recognize native DARC on cells. It inhibits P. vivax invasion <strong>of</strong> erythrocytes and displaces interleukin-8 bound to DARC. The<br />
targeted epitope overlaps the well-defined DARC Fy6 epitope. K D <strong>of</strong> CA52-DARC equilibrium is sub-nanomolar, hence ideal<br />
to develop diagnostic or therapeutic compounds. Immunocapture by immobilized CA52 yielded highly purified DARC from<br />
engineered K562 cells. This first report on a VHH with specificity for a red blood cell protein exemplifies VHHs' potentialities<br />
to target, to purify, and to modulate the function <strong>of</strong> cellular markers. © <strong>2010</strong> Springer Basel AG.<br />
No.1302<br />
Author (s) : Smolarek, D.; Hattab, C.; Hassanzadeh-Ghassabeh, G.; Cochet, S.; Gutierrez, C.; De Brevern, A. G.; Udomsangpetch, R.; Picot, J.;<br />
Grodecka, M.; Wasniowska, K.; Muyldermans, S.; Colin, Y.; Le-Van-Kim, C.; Czerwinski, M.; Bertrand, O.<br />
Title : RECOMBINANT FRAGMENT OF CAMEL HEAVY CHAIN ANTIBODIES (VHH) DIRECTED AGAINST DUFFY ANTIGEN RECEPTOR FOR<br />
CHEMOKINES<br />
Source : VOX SANGUINIS, Volume: 99, Supplement: 1, Pages: 361-361, Published: JUL <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1303<br />
Author (s) : Snabboon, T., Plengpanich, W., Houngngam, N., Buranasupkajorn, P., Plengvidhya, N., Sereepapong, W., Sunthornyothin, S.,<br />
Shotelersuk, V.<br />
Title : Concurrent bilateral pheochromocytoma and thoracic paraganglioma during pregnancy<br />
Source : Endocrine, 37 (2), pp. 261-264.<br />
Document Type : Article<br />
Keywords : Bilateral pheochromocytoma; Paraganglioma; Pregnancy; VHL<br />
<strong>Abstract</strong> : Although hypertension occurring during pregnancies is not uncommon and its prognosis is generally excellent, some <strong>of</strong> its<br />
unusual causes can lead to catastrophic consequences, especially in undiagnosed cases. Here, we report a pregnant woman<br />
who presented with hypertension in her early pregnancy. It was subsequently found to be caused by bilateral<br />
pheochromocytoma. After removal <strong>of</strong> both tumors, catecholamine levels unexpectedly and unexplainably remained<br />
elevated. At 23 weeks <strong>of</strong> gestation, the fetus was found dead in utero. After the fetal death, additional studies were<br />
performed and revealed a thoracic paraganglioma. To our knowledge, this is the first report <strong>of</strong> a case <strong>of</strong> three catecholamineproducing<br />
tumors occurring concurrently during a pregnancy. Genetic analysis helped identify this unprecedented condition;<br />
the patient harbored a heterozygous missense mutation c.482G>A in exon 3 <strong>of</strong> the VHL gene, indicating von Hippel-Lindau<br />
syndrome. Physicians who care for hypertensive pregnant patients should be aware <strong>of</strong> this condition as its diagnosis would<br />
probably lead to a better outcome. © 2009 Springer Science+Business Media, LLC.<br />
No.1304<br />
Author (s) : Sobhonslidsuk, A., Intaraprasong, P., Tongprasert, S.<br />
Title : Evaluation <strong>of</strong> donors for living donor liver transplantation (LDLT)<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, 93 (5), pp. 637-641.<br />
Document Type : Article<br />
Keywords : Deceased donor; Evaluation; Liver transplantation; Living donor<br />
<strong>Abstract</strong> : Liver transplantation has been the last resort <strong>of</strong> definite treatment for decompensate cirrhosis, early-stage <strong>of</strong> hepatocellular<br />
carcinoma, and acute liver failure. Organ shortage is the major obstacle <strong>of</strong> deceased-donor liver transplantation. Since the first<br />
case <strong>of</strong> living-donor liver transplantation (LDLT), many centers around the world started the LDLT program. Living donors<br />
should be informed about the possible risk <strong>of</strong> morbidity and mortality, and later give consent for liver donation without<br />
474
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
coercion. Donor selection and evaluation have become one <strong>of</strong> the important steps prior to LDLT, aiming to exclude donors<br />
who may have high risks from LDLT and to assure that LDLT recipients will receive perfect liver grafts. In Thailand, living<br />
donors must have been blood relatives or be legally married with recipients for at least three years. Donor evaluation can be<br />
divided into three step-by-step phases. Psychological evaluation <strong>of</strong> living donors is also included in pre-transplant<br />
assessment.<br />
No.1305<br />
Author (s) : Soe, A.N., Tansuphasawadikul, S., Phonrat, B., Boonpok, L., Tepsupa, S., Japrasert, C., Maek-a-nantawat, W.<br />
Title : Early viral suppression predicting long-term treatment success among HIV patients commencing NNRTI-based antiretroviral<br />
therapy<br />
Source : Journal <strong>of</strong> Antivirals and Antiretrovirals, 2 (2), pp. 33-37.<br />
Document Type : Article<br />
Keywords : ART; HIV; NNRTI; Toxicity; Treatment outcomes<br />
<strong>Abstract</strong> : Non-nucleoside reverse transcriptase inhibitor (NNRTI) -based antiretroviral therapy (ART) regimens have been recommended<br />
and widely used in resource-limited settings because <strong>of</strong> their reliable efficacy, low pill burden, and low cost. This study<br />
sought to determine outcomes and toxicities <strong>of</strong> NNRTI-based ART over a period <strong>of</strong> 208 weeks. A total <strong>of</strong> 244 HIV/AIDS Thai<br />
patients with a mean (±SD) age <strong>of</strong> 36 (±8.1) years initiated NNRTI-based ART in 2004. The median (inter-quartile range)<br />
baseline CD4 cell counts and HIV RNA levels were 34 (13-101) cells/mm3 and 5.4 (4.96-5.79) log copies/ml, respectively. At<br />
week 208, 84.6% <strong>of</strong> patients achieved HIV RNA loads <50 copies/ml, 88.5% continued NNRTI based regimens, 6.1%<br />
developed virologic resistance to NNRTIs, and 3.3% lost to follow up. Baseline CD4<50 cell/mm3 (p=0.019), and viral load<br />
≥50 copies/ml at 6 months post-ARV (p<0.001) were associated with treatment failure. At the end <strong>of</strong> the study, 39.8%<br />
lipoatrophy and 35.7% hyperlipidemia were identified. In conclusion, NNRTI-based regimens result in high virologic success;<br />
early undetectable viral load is key to predicting long-term virologic success. © <strong>2010</strong> Soe AN, et al.<br />
No.1306<br />
Author (s) : Sojikul, P., Kongsawadworakul, P., Viboonjun, U., Thaiprasit, J., Intawong, B., Narangajavana, J., Svasti, M.R.J.<br />
Title : AFLP-based transcript pr<strong>of</strong>iling for cassava genome-wide expression analysis in the onset <strong>of</strong> storage root formation<br />
Source : Physiologia Plantarum, 140 (2), pp. 189-298.<br />
Document Type : Article<br />
Keywords : complementary DNA; amplified fragment length polymorphism, article, cassava, chemistry, DNA sequence, expressed<br />
sequence tag, gene expression pr<strong>of</strong>iling, gene expression regulation, genetics, growth, development and aging, molecular<br />
genetics, nucleotide sequence, plant genome, plant root, reverse transcription polymerase chain reaction; Amplified Fragment<br />
Length Polymorphism Analysis, DNA, Complementary, Expressed Sequence Tags, Gene Expression Pr<strong>of</strong>iling, Gene Expression<br />
Regulation, Developmental, Gene Expression Regulation, Plant, Genome, Plant, Manihot, Molecular Sequence Data, Plant<br />
Roots, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, DNA; Manihot esculenta, Solanum tuberosum<br />
<strong>Abstract</strong> : Cassava (Manihot esculenta Crantz) is a root crop that accumulates large quantities <strong>of</strong> starch, and it is an important source <strong>of</strong><br />
carbohydrate. Study on gene expressions during storage root development provides important information on storage root<br />
formation and starch accumulation as well as unlock new traits for improving <strong>of</strong> starch yield. cDNA-Amplified Fragment<br />
Length Polymorphism (AFLP) was used to compare gene expression pr<strong>of</strong>iles in fibrous and storage roots <strong>of</strong> cassava cultivar<br />
Kasetsart 50. Total <strong>of</strong> 155 differentially expressed transcript-derived fragments with undetectable or low expression in leaves<br />
were characterized and classified into 11 groups regarding to their functions. The four major groups were no similarity (20%),<br />
hypothetical or unknown proteins (17%), cellular metabolism and biosynthesis (17%) and cellular communication and<br />
signaling (14%). Interestingly, sulfite reductase (MeKD82), calcium-dependent protein kinase (CDPK) (MeKD83), ent-kaurene<br />
synthase (KS) (MeKD106) and hexose transporter (HT) (MeKD154) showed root-specific expression patterns. This finding is<br />
consistent with previously reported genes involved in the initiation <strong>of</strong> potato tuber. Semi-quantitative reverse transcription<br />
polymerase chain reaction <strong>of</strong> early-developed root samples confirmed that those four genes exhibited significant expression<br />
with similar pattern in the storage root initiation and early developmental stages. We proposed that KS and HT may involve in<br />
transient induction <strong>of</strong> CDPK expression, which may play an important role in the signaling pathway <strong>of</strong> storage root initiation.<br />
Sulfite reductase, on the other hand, may involve in storage root development by facilitating sulfur-containing protein<br />
biosynthesis or detoxifying the cyanogenic glucoside content through aspartate biosynthesis. Copyright © Physiologia<br />
Plantarum <strong>2010</strong>.<br />
475
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.1307<br />
Author (s) : Sokal, D.C., Vach, T.H., Nanda, K., McCann, M.F., Weiner, D.H., Drobnes, C., Rochanawutanon, M., Duc, N.B., Loan, N.D.<br />
Title : Quinacrine sterilization and gynecologic cancers: A case-control study in Northern Vietnam<br />
Source : Epidemiology, 21 (2), pp. 164-171.<br />
Document Type : Article<br />
Keywords : mepacrine; adult, article, cancer risk, case control study, controlled study, disease association, drug exposure, female, female<br />
sterilization, gynecologic cancer, human, leiomyosarcoma, major clinical study, ovary cancer, priority journal, uterine cervix<br />
cancer, uterus cancer, Viet Nam, Wart virus; Age Factors, Case-Control Studies, Confidence Intervals, Female, Genital<br />
Neoplasms, Female, Humans, Leiomyosarcoma, Middle Aged, Odds Ratio, Ovarian Neoplasms, Quinacrine, Registries, Risk<br />
Factors, Sterilization, Reproductive, Uterine Cervical Neoplasms, Uterine Neoplasms, Vietnam<br />
<strong>Abstract</strong> : BACKGROUND:: Over 100,000 women worldwide have been sterilized by insertion <strong>of</strong> quinacrine into the uterus to induce<br />
tubal scarring. Concern has been expressed about possible carcinogenicity, and specifically the risk <strong>of</strong> uterine cancer.<br />
METHODS:: From 2001 through 2006, we conducted a population-based, case-control study <strong>of</strong> gynecologic cancers in 12<br />
provinces in northern Vietnam, where relatively large numbers <strong>of</strong> women had received quinacrine. Cases <strong>of</strong> incident cervical,<br />
ovarian, and uterine cancer were identified at provincial hospitals or at referral hospitals in Hanoi. For each case, 3 age-and<br />
residence-matched controls were randomly selected from the population registries <strong>of</strong> the case's home community. RESULTS::<br />
The prevalence <strong>of</strong> quinacrine exposure was 1.2% among cases and 1.1% among controls. For cervical cancer, analysis <strong>of</strong> 606<br />
cases (9 exposed) and their 1774 matched controls (18 exposed) produced an odds ratio <strong>of</strong> 1.44 (95% confidence interval =<br />
0.59-3.48) (adjusted for several covariates including human papillomavirus risk score). For ovarian cancer, based on 262 cases<br />
(3 exposed) and 755 controls (8 exposed) and adjusted for age and number <strong>of</strong> years <strong>of</strong> ovulation, the odds ratio was 1.26<br />
(0.21-5.45). For uterine cancer, none <strong>of</strong> the cases-including 23 cases <strong>of</strong> leiomyosarcoma-was exposed to quinacrine. The 95%<br />
confidence interval, based on 161 cases (none exposed) and 470 controls (7 exposed) and adjusted only for age, was 0-1.85.<br />
CONCLUSION:: We found no evidence <strong>of</strong> a relationship between quinacrine sterilization and gynecologic cancer. Copyright ©<br />
<strong>2010</strong> by Lippincott Williams & Wilkins.<br />
No.1308<br />
Author (s) : Somboonna, N., Mangkalanan, S., Udompetcharaporn, A., Krittanai, C., Sritunyalucksana, K., Flegel, T.W.<br />
Title : Mud crab susceptibility to disease from white spot syndrome virus is species-dependent<br />
Source : BMC Research Notes, 3, art. no. 315, .<br />
Document Type : Article<br />
Keywords : Decapoda (Crustacea), Scylla, Scylla olivacea, Scylla paramamosain, Scylla serrata, Shrimp white spot syndrome virus<br />
<strong>Abstract</strong> : Background: Based on a report for one species (Scylla serrata), it is widely believed that mud crabs are relatively resistant to<br />
disease caused by white spot syndrome virus (WSSV). We tested this hypothesis by determining the degree <strong>of</strong> susceptibility in<br />
two species <strong>of</strong> mud crabs, Scylla olivacea and Scylla paramamosain, both <strong>of</strong> which were identified by mitochondrial 16 S<br />
ribosomal gene analysis. We compared single-dose and serial-dose WSSV challenges on S. olivacea and S. paramamosain.<br />
Findings: In a preliminary test using S. olivacea alone, a dose <strong>of</strong> 1 × 106 WSSV copies/g gave 100% mortality within 7 days. In<br />
a subsequent test, 17 S. olivacea and 13 S. paramamosain were divided into test and control groups for challenge with WSSV<br />
at 5 incremental, biweekly doses starting from 1 × 104 and ending at 5 × 106 copies/g. For 11 S. olivacea challenged, 3<br />
specimens died at doses between 1 × 105 and 5 × 105 copies/g and none died for 2 weeks after the subsequent dose (1 ×<br />
106 copies/g) that was lethal within 7 days in the preliminary test. However, after the final challenge on day 56 (5 × 106<br />
copies/g), the remaining 7 <strong>of</strong> 11 S. olivacea (63.64%) died within 2 weeks. There was no mortality in the buffer-injected<br />
control crabs. For 9 S. paramamosain challenged in the same way, 5 (55.56%) died after challenge doses between 1 × 104<br />
and 5 × 105 copies/g, and none died for 2 weeks after the challenge dose <strong>of</strong> 1 × 106 copies/g. After the final challenge (5 ×<br />
106 copies/g) on day 56, no S. paramamosain died during 2 weeks after the challenge, and 2 <strong>of</strong> 9 WSSV-infected S.<br />
paramamosain (22.22%) remained alive together with the control crabs until the end <strong>of</strong> the test on day 106. Viral loads in<br />
these survivors were low when compared to those in the moribund crabs. Conclusions: S. olivacea and S. paramamosain<br />
show wide variation in response to challenge with WSSV. S. olivacea and S. paramamosain are susceptible to white spot<br />
disease, and S. olivacea is more susceptible than S. paramamosain. Based on our single-challenge and serial challenge results,<br />
and on previous published work showing that S. serrata is relatively unaffected by WSSV infection, we propose that<br />
susceptibility to white spot disease in the genus Scylla is species-dependent and may also be dose-history dependent. In<br />
practical terms for shrimp farmers, it means that S. olivacea and S. paramamosain may pose less threat as WSSV carriers than<br />
S. serrata. For crab farmers, our results suggest that rearing <strong>of</strong> S. serrata would be a better choice than S. paramamosain or S.<br />
476
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
olivacea in terms <strong>of</strong> avoiding losses from seasonal outbreaks <strong>of</strong> white spot disease. © <strong>2010</strong> Somboonna et al; licensee<br />
BioMed Central Ltd.<br />
No.1309<br />
Author (s) : Somchaipeng, T., Kruatong, T.<br />
Title : The development <strong>of</strong> thai pre-service mathematics teachers' pedagogical content knowledge: A series teaching case<br />
Source : <strong>International</strong> Journal <strong>of</strong> Learning, 17 (7), pp. 257-276.<br />
Document Type : Article<br />
Keywords : Pre-service mathematics teacher pedagogical content knowledge; Series<br />
<strong>Abstract</strong> : One important component <strong>of</strong> pedagogical content knowledge (PCK) is knowledge <strong>of</strong> strategies and representations for<br />
teaching particular topics. Aimed to enhance this component <strong>of</strong> pre-service mathematics teachers, the abstract and symbolic<br />
concept <strong>of</strong> series is used as a learning context <strong>of</strong> a learning unit which is called "PCK+series unit", which was developed. The<br />
teaching and learning process <strong>of</strong> this unit was based on the constructivist approach to foster atmospheres <strong>of</strong> mathematics<br />
learning through key features <strong>of</strong> a constructivist view <strong>of</strong> learning, and was implemented while enhancing pre-service teachers'<br />
PCK in instructional designs and teaching <strong>of</strong> series. The unit was implemented (8 weeks) with 23 pre-service mathematics<br />
teachers in the third year <strong>of</strong> attending selected topics in a mathematics instruction course. The collection <strong>of</strong> data involved a<br />
pre-post questionnaire, weekly journals, and video recording <strong>of</strong> series lesson plan presentation sessions with their respective<br />
mentors. At the beginning <strong>of</strong> the PCK+series unit implementation, most pre-service teachers were unable to employ<br />
appropriate teaching strategies required to explain series concepts. Most <strong>of</strong> them planned to teach by showing various series<br />
formulas and giving some examples <strong>of</strong> problem solving through a traditional teaching approach. After the implementation,<br />
learning components <strong>of</strong> PCK and several activities including brief hands-on activities helped pre-service teachers to realize<br />
that moving from the constructivist learning theory into classroom practice is necessary for their future students. The results<br />
support a growing awareness among pre-service teachers concerning the need to understand students' prior knowledge and<br />
relate it with new learning experiences provided in teaching and learning situation; for example using guided questions and<br />
designing series models to help students comprehend and build series' formulas from their own ideas. © Common Ground,<br />
Tongta Somchaipeng, Tussatrin Kruatong.<br />
No.1310<br />
Author (s) : Somcharit L., Keorochana K., Muangman P., Chunhasuwankul R., Siritongtaworn P., Pongnumkul C.<br />
Title : Thoracic trauma at Siriraj Hospital 1997-2006.<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 1, January <strong>2010</strong>, Pages 73-76<br />
Document Type : Article<br />
Keywords : Thoracic trauma<br />
<strong>Abstract</strong> : Thoracic trauma is a common injury that has a high mortality rate. Fortunately, most can be treated by a simple maneuver as<br />
intercostal drainage (79.4%). During the decade 1997-2006, there were 897 admitted patients in the Trauma division <strong>of</strong> Siriraj<br />
Hospital. Most were men (85.5%) and the common age group was 21-30 years. Blunt trauma was the major type <strong>of</strong> injury<br />
(58.9%) and traffic accidents were common causes. Abdominal injury was the most common associated injury. After the<br />
management was improved, the overall mortality rate was reduced from 7.0% to 5.1%. Today, minimally invasive surgery<br />
such as laparoscopy can reduce hospital stays and pain in patients with thoracoabdominal injury.<br />
No.1311<br />
Author (s) : Sompradeekul, S., Minipan, M.<br />
Title : Clinical features and management <strong>of</strong> chronic thromboembolic pulmonary hypertension in Thai.<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand = Chotmaihet thangphaet, 93 Suppl 1, pp. S79-85.<br />
Document Type : Article<br />
Keywords : anticoagulant agent, heparin; adult, aged, article, computer assisted tomography, cross-sectional<br />
study, endarterectomy, female, human, incidence, lung, lung embolism, male, middle aged, pathophysiology, pulmonary<br />
artery, pulmonary hypertension, radiography, retrospective study, risk factor, Thailand, thromboembolism, treatment<br />
outcome, vascularization; Adult, Aged, Anticoagulants, Cross-Sectional<br />
Studies, Endarterectomy, Female, Heparin, Humans, Hypertension, Pulmonary, Incidence, Lung, Male, Middle Aged, Pulmonary<br />
Artery, Pulmonary Embolism, Retrospective Studies, Risk Factors, Thailand, Thromboembolism, Tomography, X-Ray<br />
Computed, Treatment Outcome<br />
477
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : BACKGROUND: The incidence and clinical features <strong>of</strong> chronic thromboembolic pulmonary hypertension (CTEPH) in Thailand<br />
are unknown. This may be due to the difficulties in diagnosis, poor disease recognition and non-specific presenting symptoms<br />
and signs. With the new revolution <strong>of</strong> diagnostic tools and advanced knowledge about this condition, CTEPH is now<br />
discovered much more <strong>of</strong>ten. STUDY DESIGN: Retrospective cross-sectional study. OBJECTIVE: To evaluate common risk<br />
factors, symptoms, signs, commonly used investigations, treatment and results <strong>of</strong> treatment in Thai patients with CTEPH.<br />
MATERIAL AND METHOD: All patients with confirmed CTEPH diagnoses were selected for analysis in this study. We evaluated<br />
characteristics <strong>of</strong> patients with CTEPH at Siriraj hospital. All CTEPH patients' data included demographics, symptoms, signs,<br />
investigations, treatments, outcome and risk factors such as malignancy; previous deep vein thrombosis, immobilization, and<br />
congenital thrombophilia were recorded. STATISTICAL ANALYSIS: The descriptive statistics were used in this study. Categorical<br />
data and continuous data presented in term <strong>of</strong> percent and mean with standard deviation respectively. RESULTS: fourteen<br />
patients had confirmed diagnoses <strong>of</strong> CTEPH, five patients were male and 9 were female. The age <strong>of</strong> patients varied from 28<br />
to 79-years-old. Mean age was 55-years-old. The most common presenting symptoms and signs were dyspnea (100%),<br />
followed by leg edema (78.6%), tachypnea (71.4%) and accentuation <strong>of</strong> pulmonic component <strong>of</strong> second heart sound (loud<br />
P2) (57.1%). At the time <strong>of</strong> diagnosis, four out <strong>of</strong> 14 patients were in NYHA class II, ten were in NYHA class III, and none <strong>of</strong><br />
them was in NYHA class IV. Idiopathic CTEPH was found in 54.1% <strong>of</strong> patients. Congenital and acquired thrombophilia were the<br />
frequent risk factors found in our series. The most frequent radiographic abnormalities included enlarged pulmonary trunk,<br />
right atrium/right ventricle (RA/RV) dilatation, and cardiomegaly followed by prominent pulmonary trunk. Echocardiography<br />
findings were elevated right ventricular systolic pressure (RVSP); ranges varied from 60 mmHg to 137 mmHg (mean was 107.48<br />
mmHg). The most common finding <strong>of</strong> arterial blood gas in APE patients was respiratory alkalosis with hypoxemia. The most<br />
frequent diagnostic tool used was ventilation-perfusion lung scan. Treatment <strong>of</strong> CTEPH patients was by anticoagulant,<br />
pulmonary thromboendarterectomy and inferior vena cava filter insertion. The success rate <strong>of</strong> surgical<br />
thromboendarterectomy in our institute was about 80%. CONCLUSION: Characteristics <strong>of</strong> CTEPH patients are unique and<br />
different from acute pulmonary embolism in aspects <strong>of</strong> clinical presentations, risk factors, investigation, common findings,<br />
treatment and outcome.<br />
No.1312<br />
Author (s) : Somprasong, N., McMillan, I., Karkh<strong>of</strong>f-Schweizer, R.R., Mongkolsuk, S., Schweizer, H.P.<br />
Title : Methods for genetic manipulation <strong>of</strong> Burkholderia gladioli pathovar cocovenenans<br />
Source : BMC Research Notes, 3, art. no. 308, .<br />
Document Type : Article<br />
Keywords : Bacteria (microorganisms), Burkholderia gladioli, Negibacteria<br />
<strong>Abstract</strong> : Background. Burkholderia gladioli pathovar cocovenenans (BGC) is responsible for sporadic food-poisoning outbreaks with high<br />
morbidity and mortality in Asian countries. Little is known about the regulation <strong>of</strong> virulence factor and toxin production in<br />
BGC, and studies in this bacterium have been hampered by lack <strong>of</strong> genetic tools. Findings. Establishment <strong>of</strong> a comprehensive<br />
antibiotic susceptibility pr<strong>of</strong>ile showed that BGC strain ATCC33664 is susceptible to a number <strong>of</strong> antibiotics including<br />
aminoglycosides, carbapenems, fluoroquinolones, tetracyclines and trimethoprim. In this study, we established that<br />
gentamicin, kanamycin and trimethoprim are good selection markers for use in BGC. Using a 10 min method for preparation <strong>of</strong><br />
electrocompetent cells, the bacterium could be transformed by electroporation at high frequencies with replicative plasmids<br />
containing the pRO1600-derived origin <strong>of</strong> replication. These plasmids exhibited a copy number <strong>of</strong> > 100 in BGC. When coconjugated<br />
with a transposase expressing helper plasmid, mini-Tn7 vectors inserted site- and orientation-specifically at a<br />
single glmS-associated insertion site in the BGC genome. Lastly, a Himar1 transposon was used for random transposon<br />
mutagenesis <strong>of</strong> BGC. Conclusions. A series <strong>of</strong> genetic tools previously developed for other Gram-negative bacteria was<br />
adapted for use in BGC. These tools now facilitate genetic studies <strong>of</strong> this pathogen and allow establishment <strong>of</strong> toxin<br />
biosynthetic pathways and their genetic regulation. © <strong>2010</strong> Schweizer et al; licensee BioMed Central Ltd.<br />
No.1313<br />
Author (s) : Somsuk, N., Punnakitikashem, P., Laosirihongthong, T.<br />
Title : Determining enabling factors <strong>of</strong> <strong>University</strong> Technology Business Incubation program: Resource-based view theory<br />
Source : IEEM<strong>2010</strong> - IEEE <strong>International</strong> Conference on Industrial Engineering and Engineering Management, art. no. 5674238, pp. 1032-<br />
1037.<br />
Document Type : Conference Paper<br />
Keywords : Analytic hierarchy process; Resource-based view; <strong>University</strong> Technology Business Incubator<br />
478
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : <strong>University</strong> Technology Business Incubation (UTBI) is a program developed for an entrepreneurial company by providing a<br />
variety <strong>of</strong> resources and services to a selected start-up or entrepreneurial groups in early-stage technology-related ventures.<br />
To promote the success <strong>of</strong> UTBI program, it is important for management team to understand the necessary resources<br />
required by the program. The purpose <strong>of</strong> this paper is to determine enabling factors with respect to company resources in<br />
enhancing the success <strong>of</strong> UTBI. Resource-Based View (RBV) theory was considered as a grounded theory to develop those<br />
enabling factors. After reviewing literature on UTBIs and RBV theory, four categories <strong>of</strong> resources were identified. These are<br />
human, technological, financial, and organizational resources. Then, the attributes representing each category were assigned<br />
using the Q-sort technique. Finally, the model <strong>of</strong> analytical hierarchy decision making process is proposed to examine the<br />
relative importance <strong>of</strong> each resource to the success <strong>of</strong> UTBI program. Panel <strong>of</strong> experts will be carried out in the next stage <strong>of</strong><br />
this research project. ©<strong>2010</strong> IEEE.<br />
No.1314<br />
Author (s) : Songneam, N., Phokharatkul, P., Kimpan, C.<br />
Title : Radial Inverse Force Histograms: A new algorithm for content-based image retrieval<br />
Source : <strong>2010</strong> <strong>International</strong> Conference on Computer, Mechatronics, Control and Electronic Engineering, CMCE <strong>2010</strong>, 6, art.<br />
no. 5609929, pp. 29-32.<br />
Document Type : Conference Paper<br />
Keywords : Content-based image retrieval; Membership matching score; Radial inverse force histogram<br />
<strong>Abstract</strong> : This paper proposes a novel method, called "Radial Inverse Force Histograms (RIFH)". The principle <strong>of</strong> this method uses the<br />
gravitational forces in Newton's theory to represent the texture <strong>of</strong> image in histogram pattern. In a far and away distance the<br />
gravitational forces were reduced. In spite <strong>of</strong> this region has influence to retrieve the image. In order to overcome this<br />
problem, this research uses RIFH. The method can be used for retrieval invariant images (translation, rotation, and scaling). In<br />
experimental with the grey level image, this research studies and compare with the distance histograms method and angular<br />
histograms method. It found that, the RIFH has a better performance than other approaches. © <strong>2010</strong> IEEE.<br />
No.1315<br />
Author (s) : Songsaeng, D., Geibprasert, S., Willinsky, R., Tymianski, M., Terbrugge, K.G., Krings, T.<br />
Title : Impact <strong>of</strong> anatomical variations <strong>of</strong> the circle <strong>of</strong> Willis on the incidence <strong>of</strong> aneurysms and their recurrence rate following<br />
endovascular treatment<br />
Source : Clinical Radiology, 65 (11), pp. 895-901.<br />
Document Type : Article<br />
Keywords : adult, aged, anatomical variation, anterior communicating artery, aplasia, artery diameter, artery dysplasia, article, basilar<br />
artery, brain angiography, brain artery aneurysm, brain circulus arteriosus, coil embolization, female, follow up, human, major<br />
clinical study, male, posterior communicating artery, priority journal, recurrent disease; Aneurysm, Ruptured, Cerebral<br />
Angiography, Circle <strong>of</strong> Willis, Embolization, Therapeutic, Female, Humans, Incidence, Intracranial Aneurysm, Male, Middle<br />
Aged, Recurrence, Treatment Outcome<br />
<strong>Abstract</strong> : Aim: To analyse the impact <strong>of</strong> anatomical variations <strong>of</strong> the parent arteries on the incidence and recurrence rate following coil<br />
embolization <strong>of</strong> aneurysms <strong>of</strong> the anterior (AcoA), posterior communicating artery (PcoA) and basilar artery (BA) tip. Methods:<br />
Two hundred and two (96 AcoA, 67 PcoA, and 29 BA) aneurysms in 200 patients were treated with coil embolization between<br />
January 2000 and April 2008. Parent artery variations at each location were classified as: AcoA: A1 aplasia versus hypoplasia<br />
versus symmetrical size; PcoA: foetal origin versus medium versus small size, BA: cranial versus caudal versus asymmetrical<br />
fusion. The incidence <strong>of</strong> aneurysms and difference between recurrence rates for each group were recorded on follow-up.<br />
Results: AcoA, PcoA, and BA aneurysms were more <strong>of</strong>ten associated with embryonically earlier vessel wall dispositions (A1<br />
aplasia, foetal PcoA, asymmetrical fusion). Two <strong>of</strong> these variations were also associated with aneurysm recurrence following<br />
coil embolization: asymmetrical A1 segment (p = 0.01), and asymmetrical BA tip (p = 0.02). Conclusions: AcoA, PcoA, and BA<br />
tip aneurysms tend to occur more <strong>of</strong>ten in anatomically variant parent artery dispositions, some <strong>of</strong> which are related to<br />
aneurysm recurrence following coil embolization. This may relate to a more fragile vessel disposition as it is not fully matured<br />
or to altered haemodynamics secondary to the anatomical variations. © <strong>2010</strong> The Royal College <strong>of</strong> Radiologists. Published by<br />
Elsevier Ltd. All rights reserved.<br />
No.1316<br />
Author (s) : Songsaeng, D., Srivatanakul, K., Krings, T., Geibprasert, S., Ozanne, A., Lasjaunias, P.<br />
479
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Symptomatic spontaneous vertebrobasilar dissections in children: Review <strong>of</strong> 29 consecutive cases: Clinical article<br />
Source : Journal <strong>of</strong> Neurosurgery: Pediatrics, 6 (3), pp. 233-243.<br />
Document Type : Review<br />
Keywords : Cerebral ischemia; Childhood aneurysm; Dissecting aneurysm; Pediatric stroke; Subarachnoid hemorrhage; Vertebrobasilar<br />
artery dissection<br />
<strong>Abstract</strong> : Object. The purpose <strong>of</strong> this study is to analyze the clinical presentation, morphological characteristics, angio-architecture, and<br />
outcome <strong>of</strong> vertebrobasilar dissection (VBD) in the pediatric population. Methods. The authors retrospectively reviewed 29<br />
consecutive cases involving children younger than 16 years <strong>of</strong> age who were diagnosed with symptomatic VBDs. Data were<br />
gathered with respect to the patient's age, sex, clinical history, associated underlying disease, and symptoms (headache,<br />
vertigo) as well as the location <strong>of</strong> the dissection and the imaging appearance. Results. The patients' mean age was 8.24 years<br />
(range 2 months - 15 years). There was an overall 3:1 male predominance, although among children older than 8 years, girls<br />
and boys were similarly affected. Hemorrhagic dissections occurred in 10 <strong>of</strong> 29 cases. In nonhemorrhagic dissections, stroke<br />
occurred in 16 cases, with the most common presenting symptoms being headaches and vertigo; in the other 3 cases, mass<br />
effect due to a chronic dissecting aneurysm was present. In 7 children an underlying vessel wall disease was found. The<br />
location <strong>of</strong> the dissection was extradural in 11 cases and intradural in the remainder. There was no preference with respect to<br />
side. The basilar artery was affected in 9 patients. Conclusions. The imaging appearance and clinical presentation <strong>of</strong><br />
symptomatic VBDs in the pediatric population differs from that in adults. Boys are more <strong>of</strong>ten affected, especially at younger<br />
ages, and hemorrhagic presentation is more common, presumably owing to the fact that the basilar artery is more commonly<br />
involved. Depending on the pathogenetic mechanism underlying the dissection, different clinical symptoms will evolve,<br />
necessitating individually tailored treatment.<br />
No.1317<br />
Author (s) : Songthaveesin, C.<br />
Title : THE NEURAL STEM CELLS OF THE RETINA IN BOVINE<br />
Source : JOURNAL OF NEUROCHEMISTRY, Volume: 115, Supplement: 1, Pages: 48-48, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1318<br />
Author (s) : Songthaveesin, C.; Kotchabphakdi, N.<br />
Title : SCRATCHING SUBCULTURES OF BOVINE NEURAL STEM CELLS<br />
Source : JOURNAL OF NEUROCHEMISTRY, Volume: 115, Supplement: 1, Pages: 48-48, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1319<br />
Author (s) : Sonthayanon, P., Peacock, S.J., Chierakul, W., Wuthiekanun, V., Blacksell, S.D., Holden, M.T.G., Bentley, S.D., Feil, E.J., Day, N.P.J.<br />
Title : High rates <strong>of</strong> homologous recombination in the mite endosymbiont and opportunistic human pathogen orientia<br />
tsutsugamushi<br />
Source : PLoS Neglected Tropical Diseases, 4 (7), art. no. e752, .<br />
Document Type : Article<br />
Keywords : bacterial DNA, gene product, outer membrane protein A, protein gpsA, protein mdh, protein NrdB, protein nuoF, protein<br />
ppdK, protein sucB, protein sucD, unclassified drug; article, Australia, bacterial strain, bacterium culture, bacterium<br />
isolate, controlled study, DNA extraction, endosymbiont, gene amplification, genetic<br />
variability, genome, genotype, homologous recombination, housekeeping gene, human, major clinical study, microbial<br />
diversity, mite, multilocus sequence typing, nonhuman, nucleotide sequence, Orientia tsutsugamushi, phylogeny, polymerase<br />
chain reaction, scrub typhus, sequence analysis, Thailand, Wolbachia, animal, bacterium identification, chemistry, DNA<br />
fingerprinting, DNA sequence, genetic polymorphism, genetic recombination, genetics, isolation and<br />
purification, methodology, microbiology; Animals, Bacterial Typing Techniques, DNA Fingerprinting, DNA,<br />
480
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Bacterial, Humans, Mites, Orientia tsutsugamushi, Polymorphism, Genetic, Recombination, Genetic, Scrub Typhus, Sequence<br />
Analysis, DNA<br />
<strong>Abstract</strong> : Orientia tsutsugamushi is an intracellular a-proteobacterium which resides in trombiculid mites, and is the causative agent <strong>of</strong><br />
scrub typhus in East Asia. The genome sequence <strong>of</strong> this species has revealed an unprecedented number <strong>of</strong> repeat<br />
sequences, most notably <strong>of</strong> the genes encoding the conjugative properties <strong>of</strong> a type IV secretion system (T4SS). Although this<br />
observation is consistent with frequent intragenomic recombination, the extent <strong>of</strong> homologous recombination (gene<br />
conversion) in this species is unknown. To address this question, and to provide a protocol for the epidemiological<br />
surveillance <strong>of</strong> this important pathogen, we have developed a multilocus sequence typing (MLST) scheme based on 7<br />
housekeeping genes (gpsA, mdh, nrdB, nuoF, ppdK, sucD, sucB). We applied this scheme to the two published genomes, and<br />
to DNA extracted from blood taken from 84 Thai scrub typhus patients, from 20 cultured Thai patient isolates, 1 Australian<br />
patient sample, and from 3 cultured type strains. These data demonstrated that the O. tsutsugamushi population was both<br />
highly diverse [Simpson's index (95% CI) = 0.95 (0.92-0.98)], and highly recombinogenic. These results are surprising given the<br />
intracellular life-style <strong>of</strong> this species, but are broadly consistent with results obtained for Wolbachia, which is an<br />
aproteobacterial reproductive parasite <strong>of</strong> arthropods. We also compared the MLST data with ompA sequence data and noted<br />
low levels <strong>of</strong> consistency and much higher discrimination by MLST. Finally, twenty-five percent <strong>of</strong> patients in this study were<br />
simultaneously infected with multiple sequence types, suggesting multiple infection caused by either multiple mite bites, or<br />
multiple strains co-existing within individual mites. © <strong>2010</strong> Sonthayanon et al.<br />
No.1320<br />
Author (s) : Soodchomshom, B.<br />
Title : Magnetic gap effect on the tunneling conductance in a topological insulator ferromagnet/superconductor junction<br />
Source : Physics Letters, Section A: General, Atomic and Solid State Physics, 374 (34), pp. 3561-3566.<br />
Document Type : Article<br />
Keywords : Andreev reflection; Ferromagnet/superconductor junction; Spintronics; Topological insulator<br />
<strong>Abstract</strong> : The tunneling conductance on the surface <strong>of</strong> a topological-insulator-based ferromagnet/superconductor (F/S) structure is<br />
studied where S is an s-wave superconductor with superconducting order parameter ∼Δ. The conductance is calculated<br />
based on the BTK formalism. The magnetization in F is applied along the z-direction (m→= 〈0, 0, M〉) in order to induce the<br />
energy-mass gaps (m) for the Dirac electrons in the F-region. In this work, the influence <strong>of</strong> energy gap due to the magnetic<br />
field in the F-region on the conductance is emphasized. The Fermi energy mismatch between F (EFF=EF) and S (EFS=EF+U),<br />
where the gate potential U is applied to the electrode on top <strong>of</strong> S, is also considered. As a result, a biased voltage V can<br />
cause the conductance switch at eV=∆, depending on the value <strong>of</strong> the magnetic field. The conductance is found to be<br />
linearly dependent on either m or U. The slope <strong>of</strong> the curve can also be adjusted. This linear behavior in a topologicalinsulator-<br />
based F/S structure may be valuable for electronic applications <strong>of</strong> the linear-control-current devices. The tunneling<br />
conductances <strong>of</strong> the quasi-Dirac-particle in a topological-insulator-based F/S junction are quite different from those <strong>of</strong> a<br />
graphene-based F/S junction. © <strong>2010</strong> Elsevier B.V.<br />
No.1321<br />
Author (s) : Soodchomshom, B.<br />
Title : Magneto transport on the surface <strong>of</strong> a topological insulator spin valve<br />
Source : Physics Letters, Section A: General, Atomic and Solid State Physics, 374 (28), pp. 2894-2899.<br />
Document Type : Article<br />
Keywords : Dirac electron; Magnetoresistance; Spintronics; Topological insulator<br />
<strong>Abstract</strong> : The effects <strong>of</strong> the magnetization on the transport properties <strong>of</strong> a ferromagnet/barrier/ferromagnet spin valve fabricated with a<br />
topological insulator are studied. We consider two types <strong>of</strong> junctions, (i) an F1 / normal barrier (NB) / F2 junction and (ii) an F1<br />
/ magnetic barrier (FB) / F2 junction. The junctions in both cases lie in the xy-plane with the magnetizations in both<br />
ferromagnetic regions, F1 and F2 aligned in the z-direction. The charge carriers in the topological insulator have a Dirac like<br />
energy spectrum <strong>of</strong> a massive relativistic particle with the magnetization M playing the role <strong>of</strong> the mass. The gap opening is a<br />
special magneto feature <strong>of</strong> topological insulators. In an anti parallel alignment <strong>of</strong> the two magnetizations, the mass <strong>of</strong> the<br />
carriers is negative in the region where M is in the negative direction. The negative mass leads the behaviors <strong>of</strong> the magneto<br />
transport properties and the tunneling magneto resistance <strong>of</strong> these junctions to be quite different from those <strong>of</strong> graphenebased<br />
spin values. © <strong>2010</strong> Elsevier B.V. All rights reserved.<br />
481
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.1322<br />
Author (s) : Soodchomshom, B., Tang, I.-M., Hoonsawat, R.<br />
Title : Josephson current in a double ferromagnetic layer SG/F1/F2/SG graphene sandwich<br />
Source : Physica C: Superconductivity and its Applications, 470 (2), pp. 123-128.<br />
Document Type : Article<br />
Keywords : Dirac particle; Double ferromagnetic barriers; Graphene; Josephson current; SG/F/F/SG structure<br />
<strong>Abstract</strong> : The Josephson current in a double ferromagnetic layer SG/F1/F2/SG graphene junction where the SG's are graphene-based swave<br />
superconductors and the F1,2 are ferromagnetic layers <strong>of</strong> thicknesses d1,2 in which the exchange energies are Eex1,2, is<br />
studied. The Josephson current in this junction is composed <strong>of</strong> Cooper pairs formed by quasi particles which are massless<br />
Weyl-Dirac electrons. By switching the alignment <strong>of</strong> the two exchange fields, we find that the Josephson current in the<br />
junction can change from being in the π-state to being in the 0-state. For the case where the magnitudes <strong>of</strong> the exchange<br />
field strength are the same, it is seen that the phase difference dependence <strong>of</strong> the critical Josephson current in a parallel<br />
aligned junction indicated a cross over from a 0-state to π-state whenever the ferromagnetic barrier strength χex is equal to<br />
(n/2 + 0.25)π (with n = 1, 2, ...). The AP-junction for F1 = F2 behaves as if it has no the magnetic barriers, like S/N/S junction.<br />
It is also seen that the temperature dependence <strong>of</strong> the (%) magneto-Josephson current ratio defined as the difference<br />
between the critical currents <strong>of</strong> the graphene-based P-junction and AP-junctions defined as, % PS ∼ (100 %) x (IPc - IAPc) /<br />
IPc, is found to be oscillated under varying the exchange energies. The maximum value <strong>of</strong> %PS, (max)PS → -∞ as T → TC.<br />
The cause <strong>of</strong> these effects is the relativistic nature <strong>of</strong> the quasi particles in the graphene-based junctions. © 2009 Elsevier B.V.<br />
All rights reserved.<br />
No.1323<br />
Author (s) : Soodchomshom, B., Tang, I.-M., Hoonsawat, R.<br />
Title : Perfect switching <strong>of</strong> the spin polarization in a ferromagnetic gapless graphene/superconducting gapped graphene junction<br />
Source : Physica C: Superconductivity and its Applications, 470 (1), pp. 31-36.<br />
Document Type : Article<br />
Keywords : Ferromagnet/superconductor junction; Gapped graphene; Massive Dirac electron; Specular Andreev reflection; Spin<br />
transport<br />
<strong>Abstract</strong> : With the fabrication <strong>of</strong> gapped graphene, interest in the tunneling spectroscopy in graphene-based FG/SG junctions in which<br />
one side consists <strong>of</strong> a gapless ferro-magnetic graphene (FG) and the other side, <strong>of</strong> a gapped superconducting graphene (SG)<br />
has arisen. The carriers in the gapless (gapped) graphene are 2D relativistic particles having an energy spectrum given by E =<br />
sqrt(ℏ2 vF2 k2 + (mvF2)2) (where mvF2 is the gap and vF is the Fermi velocity). The spin currents in this FG/SG junction are<br />
obtained within the framework <strong>of</strong> the extended Blonder-Tinkham-Klapwijk (BTK) formalism. The effects <strong>of</strong> the<br />
superconducting energy gap in SG, <strong>of</strong> the gap mvF2 which opened in the superconducting graphene, <strong>of</strong> the exchange field in<br />
FG, <strong>of</strong> the spin-dependent specular Andreev reflection, <strong>of</strong> the effective Fermi energy (EFF) <strong>of</strong> FG and <strong>of</strong> the bias voltage<br />
across the junction (V) are simulated. It is seen that by adjusting EFF or V, the spin polarization (defined as SP(%) = 100% ×<br />
(G↑ - G↓)/(G↑ + G↓)) can be switched from a pure spin up (SP = +100%) state to pure spin down (SP = -100%) state. © 2009<br />
Elsevier B.V. All rights reserved.<br />
No.1324<br />
Author (s) : Soodchomshom, B., Tang, I.-M., Hoonsawat, R.<br />
Title : Theory <strong>of</strong> large tunneling magnetoresistance in a gapped graphene-based ferromagnetic superconductor F/(FS) junction<br />
Source : Physica C: Superconductivity and its Applications, 470 (7-8), pp. 415-420.<br />
Document Type : Article<br />
Keywords : Ferromagnetic superconductor; Gapped graphene; Massive Dirac electron; Spintronics; Switching effect; Tunneling<br />
magnetoresistance<br />
<strong>Abstract</strong> : Coexistence <strong>of</strong> superconductivity and ferromagnetism in a gapped graphene-based system (FS) is theoretically investigated.<br />
The center-<strong>of</strong>-mass momentum, P, <strong>of</strong> a Cooper pair in FS is found to be P ∼ 2 Eex / fenced(ℏ vF sqrt(1 - (m / EFS)2)), where<br />
m, Eex, EFS are the rest mass energy <strong>of</strong> the Dirac electron, exchange energy and the Fermi energy in the superconductor FS,<br />
respectively. It is unlike the nature in a conventional FFLO state where P ∼ 2 Eex / ℏ vF. This work studies the magneto<br />
effect on the transport property <strong>of</strong> a F/(FS) junction where F is a ferromagnetic gapless graphene. In this work, FS is achieved<br />
by depositing a conventional ferromagnetic s-wave superconductor on the top <strong>of</strong> gapped graphene sheet. The Zeeman<br />
splitting in FS induces spin-dependent Andreev resonance. The conductances effected by both spin-dependent specular<br />
482
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Andreev reflections and spin-dependent Andreev resonances are investigated. The interplay between the spin-dependent<br />
specular Andreev reflection in the F region and the spin-dependent Andreev resonance in the FS region causes a very large<br />
tunneling magnetoresistance |TMR| ∼ 3000% for m → EFS, possibly valuable in the graphene-based spintronic devices. This<br />
is because <strong>of</strong> the coexistence <strong>of</strong> the superconductivity and ferromagnetism in FS and the relativistic nature <strong>of</strong> electrons in<br />
graphene. © <strong>2010</strong>.<br />
No.1325<br />
Author (s) : Sookrung, N., Chaicumpa, W.<br />
Title : A revisit to cockroach allergens<br />
Source : Asian Pacific Journal <strong>of</strong> Allergy and Immunology, Volume 28, Issue 2-3, June <strong>2010</strong>, Pages 95-106<br />
Document Type : Review<br />
Keywords : Airway hyperresposiveness (AHR); Airway remodeling; Allergic rhinitis; Ariginine kinase; Atopic asthma; Atopy; Blattella<br />
germanica; Cockroach; Cockroach allergen detection; Cockroach allergen quantification; Cockroach allergens; Cockroach<br />
allergy; Cockroach avoidance; FcεRI; FcεRII; Gprotein couple receptor; IgE; IL-17; IL-9; Per a 1; Periplaneta<br />
americana; Protease activated receptors (PARs); Proteases; Th17; Th2; Th25; Th9<br />
<strong>Abstract</strong> : Among cockroaches (CR) that live in people's homes, two species, i.e., German CR (Blattella germanica) and American CR<br />
(Periplaneta americana) predominate in temperate and tropical areas, respectively. CR is an important source <strong>of</strong> inhalant<br />
indoor allergens that sensitize atopic subjects to (localized) type I hypersensitivity or atopy including allergic rhinitis and<br />
atopic asthma. In Thailand the predominant CR species is P. americana. CR allergens are found throughout CR infested<br />
houses; the number found in kitchens correlates with the degree <strong>of</strong> CR infestation while sensitization and reactivation <strong>of</strong> the<br />
allergic morbidity are likely to occur in the living room and bedroom. Levels <strong>of</strong> the CR allergens in homes <strong>of</strong> CR allergic Thais,<br />
measured by using locally made quantification test kits, revealed that the highest levels occur in dust samples collected from<br />
the wooden houses <strong>of</strong> urban slums and in the cool and dry season. CR allergens are proteins that may be derived from any<br />
anatomical part <strong>of</strong> the insect at any developmental stage. The allergens may be also from CR secretions, excretions, body<br />
washes or frass. The proteins may be the insect structural proteins, enzymes or hormones. They may exist as<br />
dimers/multimers and/or in different is<strong>of</strong>orms. Exposure to CR allergens in infancy leads to allergic morbidity later in life.<br />
Clinical symptoms <strong>of</strong> CR allergy are usually more severe and prolonged than those caused by other indoor allergens. The<br />
mechanisms <strong>of</strong> acute and chronic airway inflammation and airway hyper-responsiveness (AHR) have been addressed including<br />
specific IgE- and non-IgEmediated mechanisms, i.e., role <strong>of</strong> proteaseactivated receptor-2 (PAR2). Participation <strong>of</strong> various<br />
allergen activated-CD4+ T cells <strong>of</strong> different sublineages, i.e., Th2, Th17, Th22, Th9, Th25, Tregs/Th3 as well as invariant NKT<br />
cells, in asthma pathogenesis have been mentioned. The diagnosis <strong>of</strong> CR allergy and the allergy intervention by CR<br />
population control are also discussed.<br />
No.1326<br />
Author (s) : Sooksriwong, C.O.<br />
Title : WHICH STRATEGY CAN CONTROL THE EXPENDITURE OF HIGH-PRICE DRUGS?<br />
Source : VALUE IN HEALTH, Volume: 13, Issue: 7, Pages: A524-A524, Published: NOV <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1327<br />
Author (s) : Soongsongkiat, M., Puwastien, P., Jittinandana, S., Dee-Uam, A., Sungpuag, P.<br />
Title : Testing <strong>of</strong> folate conjugase from chicken pancreas vs. commercial enzyme and studying the effect <strong>of</strong> cooking on folate<br />
retention in Thai foods<br />
Source : Journal <strong>of</strong> Food Composition and Analysis, Volume 23, Issue 7, November <strong>2010</strong>, Pages 681-688<br />
Document Type : Article<br />
Keywords : Asparagus; Brown rice; Chicken pancreas; Effect <strong>of</strong> cooking; Egg; Folate; Folate conjugase; Folate retention; Food<br />
analysis; Food composition; Soybeans; Steamed-mackerel; Thai foods; Tri-enzyme treatment; Vitamin<br />
<strong>Abstract</strong> : Crude enzyme from chicken pancreas as a source <strong>of</strong> folate conjugase was prepared in a lyophilised form. Homogeneity,<br />
stability and activities were checked against a commercial enzyme. Subsequently, the prepared crude enzyme was used to<br />
investigate the process <strong>of</strong> folate extraction in various food matrices and study the effect <strong>of</strong> cooking on folate retention in<br />
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several Thai foods. The lyophilised enzyme was homogeneous and contained 4 μg endogenous folate per g <strong>of</strong> prepared<br />
lyophilised crude enzyme. The stability <strong>of</strong> the lyophilised enzyme and the diluted enzyme, kept at 4 °C, was at least 12<br />
months and 3 days, respectively. The activity <strong>of</strong> the prepared lyophilised folate conjugase, used at the level <strong>of</strong> 20. mg/g<br />
sample, was evaluated by a microbiological assay <strong>of</strong> total folate in brown rice, egg, whole milk powder, soybeans and<br />
asparagus. It showed comparable results to that <strong>of</strong> the commercial enzyme. Single-enzyme treatment (folate conjugase<br />
alone) was sufficient for the determination <strong>of</strong> folate content in brown rice, soybeans and asparagus. However, for egg and<br />
whole milk powder, it was necessary to apply tri-enzyme treatment as this showed a significantly higher level (at P< 0.05) <strong>of</strong><br />
total folate than that obtained using only single- or di-enzyme (protease. +. folate conjugase) treatments. Among the<br />
representative foods studied, the levels <strong>of</strong> total folate ranked in order <strong>of</strong> concentration, were soybeans > egg > asparagus ><br />
brown rice > steamed-mackerel (common form sold) (305, 117, 95, 41 and 24 μg/100. g wet weight basis, respectively). After<br />
cooking, the true retention <strong>of</strong> total folate in the cooked foods ranged from 60% in brown rice (cooked in an electric rice<br />
cooker) up to 82% in boiled asparagus. On a wet weight basis, the total folate content in boiled soybeans, boiled asparagus,<br />
boiled egg, fried mackerel and cooked brown rice was 100, 84, 72, 22 and 12 μg/100. g, respectively. One boiled egg (50. g)<br />
contributes 36 μg folate, which is equal to 18% <strong>of</strong> the Thai Recommended Daily Intake (RDI), and thus it can be considered<br />
as a good source <strong>of</strong> folate based on one serving size. A serving <strong>of</strong> boiled soybeans (70. g) or boiled asparagus (80. g) is an<br />
excellent source <strong>of</strong> folate, each contributing about 35% <strong>of</strong> the Thai RDI. © <strong>2010</strong> Elsevier Inc.<br />
No.1328<br />
Author (s) : Soonsawad, P., Xing, L., Milla, E., Espinoza, J.M., Kawano, M., Marko, M., Hsieh, C., Furukawa, H., Kawasaki, M.,<br />
Weerachatyanukul, W., Srivastava, R., Barnett, S.W., Srivastava, I.K., Cheng, R.H.<br />
Title : Structural evidence <strong>of</strong> glycoprotein assembly in cellular membrane compartments prior to alphavirus budding<br />
Source : Journal <strong>of</strong> Virology, Volume 84, Issue 21, November <strong>2010</strong>, Pages 11145-11151<br />
Document Type : Article<br />
Keywords : glycoprotein E1, glycoprotein E2; animal cell, animal cell culture, antibody labeling, article, cell structure, cell<br />
vacuole, cytopathic vacuole type II, electron tomography, intracellular space, intracellular transport, nonhuman, priority<br />
journal, protein assembly, replicon, Semliki Forest alphavirus, virion, virus envelope, virus infection, virus isolation, virus<br />
replication; Alphavirus, Animals, Biological Transport, Cell Line, Cell Membrane, Cricetinae, Electron Microscope<br />
Tomography, Glycoproteins, Vacuoles, Viral Proteins, Virus Assembly, Virus Release; Alphavirus<br />
<strong>Abstract</strong> : Membrane glycoproteins <strong>of</strong> alphavirus play a critical role in the assembly and budding <strong>of</strong> progeny virions. However,<br />
knowledge regarding transport <strong>of</strong> viral glycoproteins to the plasma membrane is obscure. In this study, we investigated the<br />
role <strong>of</strong> cytopathic vacuole type II (CPV-II) through in situ electron tomography <strong>of</strong> alphavirus-infected cells. The results<br />
revealed that CPV-II contains viral glycoproteins arranged in helical tubular arrays resembling the basic organization <strong>of</strong><br />
glycoprotein trimers on the envelope <strong>of</strong> the mature virions. The location <strong>of</strong> CPV-II adjacent to the site <strong>of</strong> viral budding<br />
suggests a model for the transport <strong>of</strong> structural components to the site <strong>of</strong> budding. Thus, the structural characteristics <strong>of</strong> CPV-<br />
II can be used in evaluating the design <strong>of</strong> a packaging cell line for replicon production. Copyright © <strong>2010</strong>, American Society for<br />
Microbiology. All Rights Reserved.<br />
No.1329<br />
Author (s) : Soontornchainaksaeng, P., Jenjittikul, T.<br />
Title : Chromosome number variation <strong>of</strong> phytoestrogen-producing Curcuma (Zingiberaceae) from Thailand<br />
Source : Journal <strong>of</strong> Natural Medicines, Volume 64, Issue 3, July <strong>2010</strong>, Pages 370-377<br />
Document Type : Article<br />
Keywords : Celata; Chromosome number; Clatifolia; Curcuma comosa; Phytoestrogen; Wan-chak-motluk<br />
<strong>Abstract</strong> : Wan-chak-motluk is a Thai local name for native species <strong>of</strong> Curcuma (Zingiberaceae) that produce phytoestrogen in their<br />
rhizomes. Wan-chak-motluk has long been used in Thai traditional medicine for treatment <strong>of</strong> illness in the uterus and ovarian<br />
hormone deficit, and therefore it is cultivated for economic purposes throughout Thailand. The rhizome morphology <strong>of</strong> wanchak-motluk<br />
is highly variable and this may be related to its medicinal properties. Thus, this study aimed to identify and<br />
classify wan-chak-motluk collected from cultivation sites in 16 provinces <strong>of</strong> Thailand. Chromosome numbers, together with<br />
inflorescent, floral, and leaf morphology, were used to separate wan-chak-motluk into five cultivars belonging to three<br />
species: C. comosa, C. elata, and C. latifolia. Curcuma comosa has short (2-5 cm) peduncle, glabrous lower leaf, and consists<br />
<strong>of</strong> two cultivars. One cultivar has a cylindrical spike 13-17 cm long and 5-8 cm wide, whereas the cylindrical spike <strong>of</strong> the<br />
other cultivar is shorter (10-15 cm) but larger (8-12 cm). The former cultivar has 2n = 42 and the latter has 2n = 63, seldom<br />
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2n = 62 or 64. Both C. elata and C. latifolia have long (10-25 cm) peduncle and pubescent lower leaf. They differ in that C.<br />
latifolia has 2n = 63 and 84 with a red path along the midrib, whereas C. elata has 2n = 63 without a red path along the<br />
midrib. This study shows that chromosome numbers can be used to accurately verify the taxonomic identification <strong>of</strong> wanchak-motluk.<br />
© The Japanese Society <strong>of</strong> Pharmacognosy and Springer <strong>2010</strong>.<br />
No.1330<br />
Author (s) : Soontrapa, P., Larbcharoensub, N., Luxameechanporn, T., Cheewaruangroj, W., Prakunhungsit, S., Sathapatayavong, B.,<br />
Chongtrakool, P., Leopairut, J.<br />
Title : Fungal rhinosinusitis: A retrospective analysis <strong>of</strong> clinicopathologic features and treatment outcomes at Ramathibodi Hospital<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 2, March <strong>2010</strong>, Pages 442-449<br />
Document Type : Article<br />
Keywords : Clinicopathologic findings; Fungal rhinosinusitis; Invasive; Non-invasive<br />
<strong>Abstract</strong> : The objective <strong>of</strong> this study was to determine the clinicopathologic findings <strong>of</strong> invasive and non-invasive fungal rhinosinusitis<br />
and to compare the features <strong>of</strong> the two diseases. The medical records <strong>of</strong> patients with invasive and noninvasive fungal<br />
rhinosinusitis at Ramathibodi Hospital between July 1999 and June 2009 were analyzed. The criterion for the diagnosis <strong>of</strong><br />
fungal rhinosinusitis was the evidence <strong>of</strong> fungal elements from histopathologic section on sinonasal specimens. The age,<br />
gender, clinical manifestations, duration <strong>of</strong> symptoms, associated diseases, laboratory data, results <strong>of</strong> mycotic culture and<br />
treatment outcomes were analyzed. The relationship between fungal rhinosinusitis and patient characteristics as well as<br />
clinical presentations were assessed. The fungus-attributable mortality rate was determined. The study included 43 cases <strong>of</strong><br />
invasive fungal rhinosinusitis and 68 cases <strong>of</strong> non-invasive fungal rhinosinusitis. There were 44 male, and 67 female patients.<br />
The mean age at diagnosis was 54.6 years (range: 5 to 86 years). A total <strong>of</strong> 70 (63.1%) were attributed to aspergillosis, 8 (7.2%)<br />
to candidiasis, 6 (5.4%) to zygomycosis, 4 (3.6%) to phaeohyphomycosis, 1 (0.9%) to pseudallescheriasis, 1 (0.9%) to<br />
entomophthoromycosis and 21 (18.9%) to nonspecific fungi. Cultures from sinonasal tissues were positive for fungus in 37 <strong>of</strong><br />
87 cases (42.5%). The clinical presentations <strong>of</strong> fungal rhinosinusitis included nasal stuffiness (27.9%), nasal discharge (27.9%),<br />
facial pain (27.9%), fever (24.3%) and headache (19.8%). One-fifth <strong>of</strong> cases had an underlying hematologic malignancy.<br />
Invasive fungal rhinosinusitis was significantly associated with hematologic malignancy and neutropenia. Fungus-attributable<br />
mortality rate was 44.2% in invasive fungal rhinosinusitis. Early antifungal therapy and surgical drainage were associated with a<br />
survival advantage.<br />
No.1331<br />
Author (s) : Soowannayan, C., Cowley, J.A., Pearson, R.D., Wallis, T.P., Gorman, J.J., Michalski, W.P., Walker, P.J.<br />
Title : Glycosylation <strong>of</strong> gp116 and gp64 envelope proteins <strong>of</strong> yellow head virus <strong>of</strong> Penaeus monodon shrimp<br />
Source : Journal <strong>of</strong> General Virology, Volume 91, Issue 10, October <strong>2010</strong>, Pages 2463-2473<br />
Document Type : Article<br />
Keywords : glycan, glycopeptidase, glycoprotein, glycoprotein gp 116, glycoprotein gp 64, lectin, n acetylgalactosamine, n<br />
acetylglucosamine, oligosaccharide, trypsin, unclassified drug, virus envelope protein;<br />
article, Nidovirales, nonhuman, nucleotide sequence, polyacrylamide gel electrophoresis, priority journal, protein<br />
binding, protein glycosylation, protein structure, shrimp, yellow head virus; Amino Acid Sequence, Animals, Electrophoresis,<br />
Polyacrylamide Gel, Glycosylation, Mass Spectrometry, Molecular Sequence Data, Penaeidae, Peptide-N4-(N-acetyl-betaglucosaminyl)<br />
Asparagine Amidase, Protein Processing, Post-Translational, Roniviridae, Staining and Labeling, Viral Envelope<br />
Proteins; Decapoda (Crustacea), Nidovirales, Okavirus, Penaeus monodon, Roniviridae, Yellow head virus<br />
<strong>Abstract</strong> : Yellow head virus (YHV) is a highly virulent pathogen <strong>of</strong> Penaeus monodon shrimp that is classified in the genus Okavirus,<br />
family Roniviridae, in the order Nidovirales. Separation <strong>of</strong> virion proteins treated with peptide-N-glycosidase-F (PNGase-F) in<br />
SDS-polyacrylamide gels and the use <strong>of</strong> glycoprotein-specific staining methods indicated that the gp116 and gp64 envelope<br />
glycoproteins possess N-linked rather than O-linked glycans. Competitive binding inhibition <strong>of</strong> lectins with various<br />
oligosaccharide specificities indicated that glycans linked to gp64 are mannose-rich, whilst glycans linked to gp116 possess<br />
terminal N-acetylgalactosamine and N-acetylglucosamine in addition to terminal mannose-type sugars. Mass spectrometry<br />
analyses <strong>of</strong> peptides generated from YHV proteins before and after deglycosylation with PNGase-F, using combinations <strong>of</strong> the<br />
endoproteinases trypsin, Asp-N and Lys-C, confirmed occupancy <strong>of</strong> six <strong>of</strong> the seven potential N-linked glycosylation sites in<br />
gp116 and three <strong>of</strong> the four potential sites in gp64. © <strong>2010</strong> CSIRO.<br />
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No.1332<br />
Author (s) : Sopalun, K., Thammasiri, K., Ishikawa, K.<br />
Title : Effects <strong>of</strong> chitosan as the growth stimulator for Grammatophyllum speciosum in vitro culture<br />
Source : Proceedings <strong>of</strong> World Academy <strong>of</strong> Science, Engineering and Technology, Volume 71, November <strong>2010</strong>, Pages 449-451<br />
Document Type : Article<br />
Keywords : Chitosan; Grammatophyllum speciosum; Growth stimulator<br />
<strong>Abstract</strong> : The effects <strong>of</strong> chitosan, a biodegradable polymer, were studied in Grammatophyllum speciosum protocorm-like bodies (PLBs)<br />
in vitro culture. The chitosan concentration <strong>of</strong> 0, 5, 10, 15, 20, 25, 50 or 100 mg/l were supplemented in half-strength<br />
Murashige and Skoog (1/2 MS) liquid or on agar media containing 2% (w/v) sucrose. The results showed that liquid medium<br />
supplemented with 15 mg/l chitosan showed the highest relative growth rate (7-fold increase) <strong>of</strong> PLBs. On 1/2 MS agar<br />
medium supplemented with 25 mg/l chitosan gave the highest relative growth rate (4-fold increase). The relative growth rate<br />
<strong>of</strong> G. speciosum PLBs on agar medium was significantly lower than that in liquid medium. Moreover, chitosan, supplemented<br />
to agar medium promoted shoot formation but not rooting. However, supplementation at too high a level, such as 100 mg/l<br />
can inhibit growth and kill PLBs.<br />
No.1333<br />
Author (s) : Sopalun, K., Thammasiri, K., Ishikawa, K.<br />
Title : Micropropagation <strong>of</strong> the Thai orchid Grammatophyllum speciosum blume<br />
Source : Plant Cell, Tissue and Organ Culture, Volume 101, Issue 2, May <strong>2010</strong>, Pages 143-150<br />
Document Type : Article<br />
Keywords : Chitosan; Flow cytometry; Grammatophyllum speciosum; Micropropagation; Protocorm-like bodies<br />
<strong>Abstract</strong> : Protocorm-like bodies (PLBs) were induced from shoot tips <strong>of</strong> Grammatophyllum speciosum, a Thai orchid. The highest<br />
frequency <strong>of</strong> PLBs (93%) were observed on explants incubated on 1/2-Murashige and Skoog (MS) liquid medium containing<br />
2% (w/v) sucrose without any plant growth regulators (PGRs). Tests with different carbon sources compared to sucrose<br />
revealed that maltose promoted the highest relative growth <strong>of</strong> G. speciosum PLBs (7-fold increase), while trehalose and<br />
sucrose yielded 5-fold and 4-fold increases, respectively. In 1/2 MS liquid medium, addition <strong>of</strong> 15 mg/l <strong>of</strong> chitosan promoted<br />
a 7-fold increase in PLB growth while 25 mg/l promoted a 4-fold increase. However, the relative growth rate in solid culture<br />
was significantly lower than that in liquid culture. In addition, chitosan supplementation in solid medium promoted shoot<br />
formation but not rooting. Plantlet regeneration was induced using a combination <strong>of</strong> NAA and BA supplementation in 1/2 MS<br />
solid medium with optimum induction shoot and root formation at 2.0 mg/l NAA and 1.0 mg/l BA. Using this protocol,<br />
approximately 8 months was required to obtain a hundred plantlets from one shoot tip. The plantlets showed no changes in<br />
ploidy when tested by flow cytometry. © <strong>2010</strong> Springer Science+Business Media B.V.<br />
No.1334<br />
Author (s) : Sopalun, K., Thammasiri, K., Ishikawa, K.<br />
Title : Vitrification-based cryopreservation <strong>of</strong> Grammatophyllum speciosum protocorms<br />
Source : Cryo-Letters, Volume 31, Issue 4, July <strong>2010</strong>, Pages 347-357<br />
Document Type : Article<br />
Keywords : Cryopreservation; Droplet-vitrification; Encapsulation-dehydration; Encapsulation-vitrification; Orchid; Protocorms<br />
<strong>Abstract</strong> : Three vitrification-based methods for the cryopreservation <strong>of</strong> Grammatophyllum speciosum protocorms were invesigated:<br />
droplet-vitrification, encapsulation-dehydration and encapsulation-vitrification. Protocorms, 0.1 cm in diameter, developed<br />
from 2-month-old germinating seeds were used. For droplet-vitrification, protocorms were precultured on filter paper soaked<br />
in half strength Murashige and Skoog medium (1/2MS) containing 0.4 M sucrose at 25 ± 2°C for 2 d, followed by soaking in<br />
loading solution (2 M glycerol and 0.4 M sucrose in 1/2MS liquid medium) for 20 min and then dehydrated with PVS2 solution<br />
[30% (w/v) glycerol, 15% (w/v) ethylene glycol and 15% (w/v) dimethyl sulfoxide in 1/2MS liquid medium containing 0.4 M<br />
sucrose at pH 5.7] for 30 min. For encapsulation-dehydration, encapsulated protocorms were precultured in 1/2MS liquid<br />
medium containing 0.4 M sucrose on a shaker (110 rpm) at 25 ± 2°C for 2 d, followed by soaking in the same loading solution<br />
for 20 min and then exposed to a sterile air-flow at 2.5 inches/water column from the laminar air-flow cabinet for 8 h. For<br />
encapsulation- vitrification, encapsulated protocorms were precultured in 1/2MS liquid medium containing 0.4 M sucrose for 1<br />
or 2 d, followed by soaking in the same loading solution for 20 min and then dehydrated with PVS2 solution for 60 min. For<br />
all three methods, preculturing with 0.4 M sucrose for 2 d resulted in a significant induction <strong>of</strong> dehydration and freezing<br />
tolerance. The cryopreservation results showed highest protocorm regrowth after droplet-vitrification (38%), followed by<br />
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encapsulation-dehydration (24%) and encapsulation-vitrification (14%). Plantlets developed from these three methods did<br />
not show any abnormal characteristics or ploidy level change when investigated by flow cytometry. © CryoLetters.<br />
No.1335<br />
Author (s) : Sornmayura, P., Siripornpitak, S., Leela-Udomlipi, S., Bunyaratvej, S.<br />
Title : Hepatocellular adenoma: A case report<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 3, March <strong>2010</strong>, Pages 393-397<br />
Document Type : Article<br />
Keywords : Contraceptive pill; Hepatocellular carcinoma; Liver cell adenoma<br />
<strong>Abstract</strong> : A case <strong>of</strong> hepatocellular adenoma (HCA) was described in a 26-year-old woman, who was a potential kidney donor for her<br />
father and denied taking the oral contraceptive pill. A hypervascular mass <strong>of</strong> 4.1 cm in largest dimension was detected in the<br />
liver segment V by computerized tomography and magnetic resonance imaging (MRI). The normal levels <strong>of</strong> liver enzymes,<br />
negative serum markers for hepatitis viruses, and non-elevated alpha-fetoprotein level were detected. The wedge resection<br />
<strong>of</strong> segment V was done with an uneventful clinical course. In Western countries, HCAs are known to occur in women in their<br />
reproductive periods. It may not be associated with oral contraceptive. Molecular biological studies disclosed three variants<br />
<strong>of</strong> HCAs, i.e., I) with mutation <strong>of</strong> HNF 1-alpha gene, II) with mutation <strong>of</strong> beta-catenin gene, and III) no mutation <strong>of</strong> the two<br />
genes. Histological correlations with the three variants as recommended by the Bordeaux group in 2007 could not be<br />
accomplished in the present study due to overlapping histological features between the variants I and III. The etiological<br />
factors <strong>of</strong> HCA are known to relate to the contraceptive pill usage in female and the anabolic-androgenic-steroid<br />
administration in male. In Thailand, the occurrence <strong>of</strong> HCA is expected to be only 0.3% <strong>of</strong> cases with hepatocellular<br />
carcinoma.<br />
No.1336<br />
Author (s) : Sornmayura, P., Visessiri, Y., Rochanawutanon, M., Sirikulchayanonta, V., Aroonroch, R., Kanoksil, W., Larbcharoensub, N.,<br />
Wongwaisayawan, S., Leopairut, J., Chalermsanyakorn, P., Worawichawong, S., Chanplakorn, N., Leelachaikul, P., Pongtippan, A.<br />
Title : Double check up <strong>of</strong> malignancy biopsy specimens for patient safety<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 11, November <strong>2010</strong>, Pages 1310-1316<br />
Document Type : Article<br />
Keywords : Malignancy; Patient safety; Quality assurance; Quality improvement; Surgical pathology<br />
<strong>Abstract</strong> : Background: The diagnostic <strong>of</strong> malignancy in biopsy specimens is very important because it guides to selected treatment<br />
option and prognostic prediction. However, biopsy specimens usually have small pieces leading to variations <strong>of</strong> the<br />
interpretation by anatomical pathologists. Objective: To detect and correct the errors or the significant discrepancies in the<br />
diagnosis <strong>of</strong> biopsy specimens before signout and to determine the frequency <strong>of</strong> anatomic pathology significant discrepancies.<br />
Design: The application <strong>of</strong> the mutually agreed work instructions (record) for the detection <strong>of</strong> errors or the significant<br />
discrepancies and their process <strong>of</strong> sign-out. The record <strong>of</strong> biopsy specimen that received a secondary check (1,959 cases,<br />
2005-2007) was analyzed. Results: After a secondary check, 53 cases <strong>of</strong> non-malignancy for any reason by a second<br />
pathologist were included. However, when using our definition on significant discrepancies, only 37 cases were considered.<br />
Another seven cases with the opinions with malignancy that were <strong>of</strong> different cell types that do harm to the patients were<br />
added. Therefore, 44 cases (2.25%) had truly significant discrepancies. Conclusion: The truly significant discrepancy frequency<br />
was 2.25% during the process <strong>of</strong> pre-sign-out secondary check <strong>of</strong> malignancy <strong>of</strong> biopsy specimens. The project has been<br />
applied as a routine daily work. It can be an innovative safety program for patient in Thailand.<br />
No.1337<br />
Author (s) : Sotthibundhu, A., Phansuwan-Pujito, P., Govitrapong, P.<br />
Title : Melatonin increases proliferation <strong>of</strong> cultured neural stem cells obtained from adult mouse subventricular zone<br />
Source : Journal <strong>of</strong> Pineal Research, Volume 49, Issue 3, October <strong>2010</strong>, Pages 291-300<br />
Document Type : Article<br />
Keywords : adult stem cell; melatonin; MT1-melatonin receptor; neurogenesis; proliferation; subventricular zone (SVZ)<br />
<strong>Abstract</strong> : Melatonin, a circadian rhythm-promoting molecule secreted mainly by the pineal gland, has a variety <strong>of</strong> biological functions<br />
and neuroprotective effects including control <strong>of</strong> sleep-wake cycle, seasonal reproduction, and body temperature as well as<br />
preventing neuronal cell death induced by neurotoxic substances. Melatonin also modulates neural stem cell (NSC) function<br />
including proliferation and differentiation in embryonic brain tissue. However, the involvement <strong>of</strong> melatonin in adult<br />
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neurogenesis is still not clear. Here, we report that precursor cells from adult mouse subventricular zone (SVZ) <strong>of</strong> the lateral<br />
ventricle, the main neurogenic area <strong>of</strong> the adult brain, express melatonin receptors. In addition, precursor cells derived from<br />
this area treated with melatonin exhibited increased proliferative activity. However, when cells were treated with luzindole, a<br />
competitive inhibitor <strong>of</strong> melatonin receptors, or pertussis toxin, an uncoupler <strong>of</strong> Gi from adenylate cyclase, melatonininduced<br />
proliferation was reduced. Under these conditions, melatonin induced the differentiation <strong>of</strong> precursor cells to<br />
neuronal cells without an upregulation <strong>of</strong> the number <strong>of</strong> glia cells. Because stem cell replacement is thought to play an<br />
important therapeutic role in neurodegenerative diseases, melatonin might be beneficial for stimulating endogenous neural<br />
stem cells. © <strong>2010</strong> John Wiley & Sons A/S.<br />
No.1338<br />
Author (s) : Sotthibundhu, A.; Phansuwan-Pujito, P.; Govitrapong, P.<br />
Title : STIMULATORY EFFECT OF MELATONIN ON NEURONAL PRECURSOR CELLS PROLIFERATION AND DIFFERENTIATION<br />
Source : JOURNAL OF NEUROCHEMISTRY, Volume: 115, Supplement: 1, Pages: 35-35, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1339<br />
Author (s) : Souris, M., Gonzalez, J.-P., Shanmugasundaram, J., Corvest, V., Kittayapong, P.<br />
Title : Retrospective space-time analysis <strong>of</strong> H5N1 Avian Influenza emergence in Thailand<br />
Source : <strong>International</strong> Journal <strong>of</strong> Health Geographics, Volume 9, 27 January <strong>2010</strong>, Article number 3<br />
Document Type : Article<br />
Keywords : avian influenza, disease incidence, disease spread, disease transmission, environmental factor, farm, poultry, spatial<br />
distribution, statistical analysis, temporal distribution, virus; Thailand; Animalia, Aves; agriculture, animal, animal<br />
disease, article, avian influenza, bird disease, cluster analysis, communicable disease, disease transmission, epidemic, health<br />
survey, Influenza virus A H5N1, isolation and purification, methodology, Monte Carlo method, poultry, retrospective<br />
study, standard, Thailand, virology; Agriculture, Animals, Communicable Diseases, Emerging, Disease Outbreaks, Influenza A<br />
Virus, H5N1 Subtype, Influenza in Birds, Monte Carlo Method, Population Surveillance, Poultry, Poultry Diseases, Retrospective<br />
Studies, Space-Time Clustering, Thailand<br />
<strong>Abstract</strong> : Background: The highly pathogenic avian influenza (HPAI) H5N1 virus remains a worldwide threat to human and animal health,<br />
while the mechanisms explaining its epizootic emergence and re-emergence in poultry are largely unknown. Data from<br />
Thailand, a country that experienced significant epidemics in poultry and has recorded suspicious cases <strong>of</strong> HPAI on a daily<br />
basis since 2004, are used here to study the process <strong>of</strong> emergence. A spatial approach is employed to describe all HPAI H5N1<br />
virus epizootics from 2004 to 2008 and to characterize the pattern <strong>of</strong> emergence: multiple independent introductions <strong>of</strong> the<br />
virus followed by moderate local spread vs. very rare emergences followed by strong local spread and rare long range<br />
diffusion jumps. Sites where epizootics originate (by foreign introduction, local persistence, or long range jump) were selected<br />
from those to which the disease subsequently spreads using a filter based on relative date and position. The spatial<br />
distribution <strong>of</strong> these selected foci was statistically analyzed, and to differentiate environmental factors from long range<br />
diffusion, we investigate the relationship <strong>of</strong> these foci with environmental exposure factors and with rearing<br />
characteristics.Results: During each wave <strong>of</strong> epizootics, the temporal occurrence <strong>of</strong> cases did not show a temporal<br />
interruption <strong>of</strong> more than a week. All foci were globally clustered; i.e., more than 90% <strong>of</strong> cases had a previous case within a<br />
10 km range and a 21 day period <strong>of</strong> time, showing a strong local spread. We were able to estimate 60 km as the maximum<br />
distance for the local farm to farm dissemination process. The remaining "emergent" cases have occurred randomly over<br />
Thailand and did not show specific location, clusters, or trends. We found that these foci are not statistically related to<br />
specific environmental conditions or land cover characteristics, and most <strong>of</strong> them may be interpreted as long range diffusion<br />
jumps due to commercial practices.Conclusion: We conclude that only a few foci appear to have been at the origin <strong>of</strong> each<br />
HPAI epidemic wave, leading to the practical action that surveillance and control must focus on farm to farm transmission<br />
rather than on emergence or wild fauna. © <strong>2010</strong> Souris et al; licensee BioMed Central Ltd.<br />
No.1340<br />
Author (s) : Sowattanangoon, N.; Hiengkaew, V.; Vongsirinavarat, M.; Chompikul, J.<br />
Title : ROLE OF BUDDHIST PRACTICES ON EMOTIONAL DISTRESS IN STROKE SURVIVORS<br />
488
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Source : INTERNATIONAL JOURNAL OF BEHAVIORAL MEDICINE, Volume: 17, Supplement: 1, Pages: 254-255, Published: AUG <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1341<br />
Author (s) : Sreta, D., Tantawet, S., Na Ayudhya, S.N., Thontiravong, A., Wongphatcharachai, M., Lapkuntod, J., Bunpapong, N., Tuanudom,<br />
R., Suradhat, S., Vimolket, L., Poovorawan, Y., Thanawongnuwech, R., Amonsin, A., Kitikoon, P.<br />
Title : Pandemic (H1N1) 2009 virus on commercial swine farm, Thailand<br />
Source : Emerging Infectious Diseases, Volume 16, Issue 10, October <strong>2010</strong>, Pages 1587-1590<br />
Document Type : Article<br />
Keywords : virus hemagglutinin; article, controlled study, epidemic, gene sequence, genetic analysis, Influenza virus A H1N1, mixed<br />
infection, nonhuman, nose smear, nucleotide sequence, pandemic influenza, phylogeny, reverse transcription polymerase<br />
chain reaction, seasonal influenza, swine influenza, Thailand, virus isolation, virus transmission; Animal<br />
Husbandry, Animals, Disease Outbreaks, Humans, Influenza A Virus, H1N1 Subtype, Influenza, Human, Orthomyxoviridae<br />
Infections, Pandemics, Seasons, Swine, Swine Diseases, Thailand<br />
<strong>Abstract</strong> : A swine influenza outbreak occurred on a commercial pig farm in Thailand. Outbreak investigation indicated that pigs were<br />
co-infected with pandemic (H1N1) 2009 virus and seasonal influenza (H1N1) viruses. No evidence <strong>of</strong> gene reassortment or pigto-human<br />
transmission <strong>of</strong> pandemic (H1N1) 2009 virus was found during the outbreak.<br />
No.1342<br />
Author (s) : Sri-Aroon, P., Chusongsang, P., Chusongsang, Y., Surinthwong, P., Butraporn, P., Lohachit, C.<br />
Title : Snails and trematode infection after indian ocean tsunami in Phang-Nga Province, southern Thailand<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 1, January <strong>2010</strong>, Pages 48-60<br />
Document Type : Article<br />
Keywords : Snails; Trematode infection; Tsunami<br />
<strong>Abstract</strong> : The tsunami and non-tsunami affected areas <strong>of</strong> Takua Pa District, Phang-Nga Province were investigated for fresh-and brackishwater<br />
snails that transmit human parasitic diseases during 2006 and 2007. Among 46 snail species found, 17 species <strong>of</strong> 8<br />
families were freshwater snails, 28 species <strong>of</strong> another 7 families were brackish-water snails, and 1 species was a land snail. Of<br />
these species, 11 freshwater snails, 4 brackish-water snails and 1 land snail were <strong>of</strong> medical importance. The fresh-water<br />
snails were Pomacea canaliculata, Pila angelica, P. gracilis, P. polita, Filopaludina (S.) martensi, F. (F.) s. polygramtna,<br />
Melatwides tuberculata, Indoplanorbis exuxtus, Radix rubiginosa, Helicorbis umbilicalis, Gyraulus convexiusculus. Four<br />
brackish-water snails were Cerithidea cingulata, C. djadjarensis, C. alata, Sermyla riqueti and Achatina fulica was the land snail.<br />
I. exutus, M. tuberculata and F. (F.) s. polygramtna harbored Xiphidio, Microcercus, Furocercus, Echinostome cercariae, and<br />
cercaria without eyespots or tail with hair. Three species <strong>of</strong> brackish-water snails, Cerithidia cingulata, C. djadjariensis, and C.<br />
alata presented with 6 types <strong>of</strong> trematode cercariae and rediae. Knowledge <strong>of</strong> medically important snails and their parasitic<br />
diseases, and prevention were given to Takua Pa people by poster, pamphlets and broadcasting through community radio.<br />
No.1343<br />
Author (s) : Sribhen, K., Piyophirapong, S., Wannasilp, N.<br />
Title : Cardiac troponin T concentrations in healthy adolescents<br />
Source : Clinica Chimica Acta, Volume 411, Issue 19-20, October <strong>2010</strong>, Pages 1542-1543<br />
Document Type : Letter<br />
Keywords : troponin T; adolescent, blood analysis, blood smear, chemoluminescence, controlled<br />
study, female, human, immunoassay, letter, male, medical student, normal value, priority journal, protein analysis, protein<br />
blood level, sensitivity and specificity, sex difference; Adolescent, Female, Humans, Immunoassay, Male, Sex<br />
Factors, Troponin T, Young Adult<br />
<strong>Abstract</strong> : –<br />
No.1344<br />
Author (s) : Srichaisiriwech, W., Wisitsoaat, A., Phokharatkul, D., Karuwan, C., Tuantranont, A., Kerdcharoen, T.<br />
Title : Electronic tongue based on modified carbon nanotube electrochemical sensor array<br />
489
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Source : ECTI-CON <strong>2010</strong> - The <strong>2010</strong> ECTI <strong>International</strong> Conference on Electrical Engineering/Electronics, Computer,<br />
Telecommunications and Information Technology, <strong>2010</strong>, Article number 5491430, Pages 544-547<br />
Document Type : Conference Paper<br />
Keywords : Current sensitivity, Electronic tongue, Functionalized, High sensitivity, Modified carbon, Oxygen plasmas, Sample pretreatment;<br />
Beverages, Carbon nanotubes, Chronoamperometry, Electrochemical oxidation, Electrochemical sensors, Information<br />
technology, Oxygen, Principal component analysis, Sensor arrays; Electronic tongues<br />
<strong>Abstract</strong> : In this work, carbon nanotube based electrochemical sensor array was developed as electronic tongue for beer discrimination<br />
without sample pretreatment using chronoamperometry. To form the sensor array, carbon nanotubes were modified by<br />
oxygen plasma and electrochemical oxidation using NaOH and KClO3 to yield electrochemical sensors with distinct<br />
characteristics. The current sensitivities from differently functionalized CNTs were analyzed by principal component analysis.<br />
Four different brands <strong>of</strong> beer, including Chang Classic, Heineken, Leo and Cheers, have been successfully distinguished by the<br />
developed electronic tongue system with high sensitivity.<br />
No.1345<br />
Author (s) : Srifeungfung, S., Tribuddharat, C., Comerungsee, S., Chatsuwan, T., Treerauthanaweeraphong, V., Rungnobhakhun, P.,<br />
Nunthapisud, P., Chokephaibulkit, K.<br />
Title : Serotype coverage <strong>of</strong> pneumococcal conjugate vaccine and drug susceptibility <strong>of</strong> Streptococcus pneumoniae isolated from<br />
invasive or non-invasive diseases in central Thailand, 2006-2009<br />
Source : Vaccine, Volume 28, Issue 19, 26 April <strong>2010</strong>, Pages 3440-3444<br />
Document Type : Article<br />
Keywords : Drug susceptibility; Pneumococcal conjugate vaccine; Serotypes; Streptococcus pneumoniae; Thailand<br />
<strong>Abstract</strong> : The serotype <strong>of</strong> 172 S. pneumoniae isolates obtained from normally sterile sites from January 2006 to February 2009 in Thai<br />
patients was evaluated. The most common serotypes were 6B, 23F, 14, 19F, and 19A in patients 65-year old. Seven-valent pneumococcal conjugated vaccine (PCV-7) covered 70.3%, 43.6%, and 43.5%<br />
<strong>of</strong> patients
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Document Type : Review<br />
Keywords : bacterial antigen; bacterial meningitis, cerebrospinal fluid, human, immunology, isolation and purification, latex agglutination<br />
test, methodology, microbiology, polymerase chain reaction, review; Antigens, Bacterial, Cerebrospinal Fluid, Humans, Latex<br />
Fixation Tests, Meningitis, Bacterial, Polymerase Chain Reaction<br />
<strong>Abstract</strong> : Rapid detection <strong>of</strong> bacterial pathogen causing meningitis is very important to guide antimicrobial therapy before the standard<br />
culture result is available. Other than gram stain, one <strong>of</strong> the most useful rapid methods is the detection <strong>of</strong> bacterial antigen<br />
in cerebrospinal fluid. This article reviewed the methods <strong>of</strong> bacterial antigen detection for diagnosis <strong>of</strong> meningitis as well as a<br />
microbiology aspect <strong>of</strong> this life-threatening disease.<br />
No.1348<br />
Author (s) : Srifuengfung, S., Chokephaibulkit, K., Tribuddharat, C., Comerungsee, S.<br />
Title : A description <strong>of</strong> antimicrobial susceptibility <strong>of</strong> Streptococcus pneumoniae-Siriraj Hospital, Thailand: 2008.<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand = Chotmaihet thangphaet, Volume 93 Suppl 5, November <strong>2010</strong>, Pages S27-34<br />
Document Type : Article<br />
Keywords : antiinfective agent; adolescent, adult, age distribution, aged, article, child, disk diffusion, drug<br />
effect, female, human, infant, isolation and purification, male, microbiology, middle aged, multidrug resistance, pneumococcal<br />
infection, preschool child, sentinel surveillance, sex ratio, statistics, Streptococcus pneumoniae, Thailand, university hospital;<br />
Adolescent, Adult, Age Distribution, Aged, Aged, 80 and over, Anti-Infective Agents, Child, Child, Preschool, Disk Diffusion<br />
Antimicrobial Tests, Drug Resistance, Multiple, Female, Hospitals, <strong>University</strong>, Humans, Infant, Male, Middle<br />
Aged, Pneumococcal Infections, Sentinel Surveillance, Sex Distribution, Streptococcus pneumoniae, Thailand, Young Adult<br />
<strong>Abstract</strong> : Streptococcus pneumoniae was isolated from 170 patient specimens at Siriraj Hospital during January-December 2008.<br />
Patients were 66% male and ranged in age from 3 months to 94 years (mean +/- SD = 38.2 +/- 31.7). The largest proportion<br />
(29.4%) <strong>of</strong> isolates were from patients older than 60 years, followed by patients aged 2-5 years (20%) and from patients less<br />
than 2 years (12.4%). Monthly isolation was highest in December (22 isolates in December compared to the average <strong>of</strong> 13<br />
isolates <strong>of</strong> the other months). Antimicrobial susceptibility for eight drugs was determined by the disk diffusion method.<br />
Overall, susceptibility was generally high to chloramphenicol (71.8%), linezolid (100%), <strong>of</strong>loxacin (93.5%) and vancomycin<br />
(100%), but less susceptible to erythromycin (35.3%), penicillin (31.1%), tetracycline (28.8%) and trimethoprim/<br />
sulfamethoxazole (24.1%). Among the 105 (62%) isolates resistant to three or more drugs, the most common resistance<br />
pattern was erythromycin-penicillin-tetracycline-trimethoprim/sulfamethoxazole, accounting for 39% <strong>of</strong> such isolates,<br />
followed by chloramphenicol-erythromycin-penicillin-tetracycline- trimethoprim/sulfamethoxazole (29.5%). The minimal<br />
inhibitory concentrations (MIC) <strong>of</strong> penicillin and cefotaxime were determined by broth microdilution. By 2008 CLSI criteria,<br />
92% and 90% <strong>of</strong> 51 sterile site isolates were penicillin and cefotaxime susceptible, including one <strong>of</strong> two meningitis cases. In<br />
contrast, <strong>of</strong> 26 non-sterile site isolates, only 26.9% and 76.9% were susceptible to penicillin and cefotaxime, respectively. The<br />
MICs <strong>of</strong> penicillin were higher for isolates from non-sterile sites than for those from sterile sites.<br />
No.1349<br />
Author (s) : Srifuengfung, S., Tribuddharat, C., Chokephaibulkit, K., Comerungsee, S.<br />
Title : Fluoroquinolone resistance in Streptococcus pneumoniae from a university hospital, Thailand.<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand = Chotmaihet thangphaet, Volume 93 Suppl 5, November <strong>2010</strong>, Pages S35-39<br />
Document Type : Article<br />
Keywords : antiinfective agent, DNA topoisomerase (ATP hydrolysing), DNA topoisomerase IV, quinolone derivative; adult, aged, amino<br />
acid sequence, antibiotic resistance, article, disk diffusion, DNA sequence, drug effect, female, genetics, human, isolation and<br />
purification, male, middle aged, molecular genetics, mutation, polymerase chain reaction, serotyping, Streptococcus<br />
pneumoniae, Thailand, university hospital; Adult, Aged, Aged, 80 and over, Amino Acid Sequence, Anti-Bacterial Agents, Disk<br />
Diffusion Antimicrobial Tests, DNA Gyrase, DNA Topoisomerase IV, Drug Resistance,<br />
Microbial, Female, Fluoroquinolones, Hospitals, <strong>University</strong>, Humans, Male, Middle Aged, Molecular Sequence<br />
Data, Mutation, Polymerase Chain Reaction, Sequence Analysis, DNA, Serotyping, Streptococcus pneumoniae, Thailand<br />
<strong>Abstract</strong> : The most frequent markers <strong>of</strong> fluoroquinolone resistance in S. pneumoniae are chromosomal mutations in the quinoloneresistance-determining<br />
regions <strong>of</strong> DNA gyrase and topoisomerase IV encoding for the gyrA, gyrB and parC, parE genes. In 2008,<br />
6.5% <strong>of</strong> the Streptococcus pneumoniae isolates in a Bangkok university hospital were resistant to <strong>of</strong>loxacin. Using PCR and<br />
DNA sequencing, we identified mutations in both the gyrA and parC genes <strong>of</strong> four <strong>of</strong>loxacin- and cipr<strong>of</strong>loxacin-resistant S.<br />
pneumoniae isolates (minimum inhibitory concentrations > 32 microg/ml). Mutations were found in the gyrA gene at positions<br />
491
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Ser81Phe, Glu85Gly, Glu85Lys and in the parC gene at position Ser79Tyr. Three isolates had mutations in both genes. Two <strong>of</strong><br />
the isolates were serotype 6B and two were serotypes not contained in currently licensed pneumococcal vaccines. This is the<br />
first report <strong>of</strong> the mechanisms <strong>of</strong> fluoroquinolone resistance in S. pneumoniae in Thailand.<br />
No.1350<br />
Author (s) : Srikuea, R., Pholpramool, C., Kitiyanant, Y., Yimlamai, T.<br />
Title : Satellite cell activity in muscle regeneration after contusion in rats<br />
Source : Clinical and Experimental Pharmacology and Physiology, Volume 37, Issue 11, November <strong>2010</strong>, Pages 1078-1086<br />
Document Type : Article<br />
Keywords : Muscle contusion; Muscle regeneration; MyoD; Myogenin; Myostatin; Satellite cells<br />
<strong>Abstract</strong> : 1. The role <strong>of</strong> satellite cells in muscle growth during development is well documented, but the involvement <strong>of</strong> these cells in<br />
muscle repair after contusion is less well known. In the present study, we investigated the time-course <strong>of</strong> satellite cell activity<br />
(from 3 h to 7 days) after contusion <strong>of</strong> rat gastrocnemius muscle using specific molecular markers for immun<strong>of</strong>luorescence<br />
and real-time polymerase chain reaction (PCR). 2. Inflammation <strong>of</strong> the injured muscle occurred within 6 h, followed by<br />
disintegration <strong>of</strong> the damaged my<strong>of</strong>ibres within 12 h. Newly formed my<strong>of</strong>ibres appeared by Day 7. 3. The number <strong>of</strong> MyoDpositive<br />
nuclei (activated satellite cells) in the injured muscle was significantly increased by 6 h, reaching a maximum by 12 h<br />
after contusion. However, the number <strong>of</strong> MyoD-positive nuclei decreased towards control levels by Day 7. Changes in the<br />
number <strong>of</strong> bromodeoxyuridine-labelled nuclei (proliferating satellite cells) paralleled the changes seen in the number <strong>of</strong><br />
MyoD-positive nuclei. Conversely, expression <strong>of</strong> myogenin protein was not apparent until Day 3 and increased further by Day<br />
7. Colabelling <strong>of</strong> MyoD and myogenin was seen in only a few cells. 4. The time-course <strong>of</strong> MyoD mRNA expression<br />
corresponded with MyoD protein expression. However, there were two peaks in myogenin mRNA expression: 6 h and Day 7<br />
after contusion. The second peak coincided with upregulation <strong>of</strong> myostatin mRNA levels. 5. The results <strong>of</strong> the present study<br />
suggest that contusion activates a homogeneous population <strong>of</strong> satellite cells to proliferate within 3 days, followed by<br />
differentiation to form new my<strong>of</strong>ibres. The latter may be regulated, in part, by myostatin. © <strong>2010</strong> The Authors. Clinical and<br />
Experimental Pharmacology and Physiology © <strong>2010</strong> Blackwell Publishing Asia Pty Ltd.<br />
No.1351<br />
Author (s) : Srinualnad, S.<br />
Title : Passing a learning curve after 200 cases <strong>of</strong> robotic assisted laparoscopic radical prostatectomy: experience from a single<br />
surgeon<br />
Source : INTERNATIONAL JOURNAL OF UROLOGY, Volume: 17, Supplement: 1, Pages: A127-A127, Published: AUG <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1352<br />
Author (s) : Srinualnad, S.<br />
Title : The functional and oncological outcomes <strong>of</strong> 242 cases <strong>of</strong> extraperitoneal laparoscopic radical prostatectomy<br />
Source : INTERNATIONAL JOURNAL OF UROLOGY, Volume: 17, Supplement: 1, Pages: A127-A127, Published: AUG <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1353<br />
Author (s) : Sriphrapradang, C.; Chailurkit, L.; Vanavanan, S.; Jongjaroenprasert, W.<br />
Title : Study <strong>of</strong> the Frequency <strong>of</strong> Thyroid Autoantibodies in Thai Patients with Autoimmune Thyroid Diseases.<br />
Source : ENDOCRINE REVIEWS, Volume: 31, Issue: 3, Supplement: 1, Published: JUN <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
492
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.1354<br />
Author (s) : Srirattana, K., Lorthongpanich, C., Laowtammathron, C., Imsoonthornruksa, S., Ketudat-Cairns, M., Phermthai, T., Nagai, T.,<br />
Parnpai, R.<br />
Title : Effect <strong>of</strong> donor cell types on developmental potential <strong>of</strong> cattle (Bos taurus) and swamp buffalo (Bubalus bubalis) cloned<br />
embryos<br />
Source : Journal <strong>of</strong> Reproduction and Development, Volume 56, Issue 1, February <strong>2010</strong>, Pages 49-54<br />
Document Type : Article<br />
Keywords : Cattle; Differential staining; Embryo development; Nuclear transfer; Swamp buffalo<br />
<strong>Abstract</strong> : This study investigated the effect <strong>of</strong> donor cell types on the developmental potential and quality <strong>of</strong> cloned swamp buffalo<br />
embryos in comparison with cloned cattle embryos. Fetal fibroblasts (FFs), ear fibroblasts (EFs), granulosa cells (GCs) and<br />
cumulus cells (CCs) were used as the donor cells in both buffalo and cattle. The cloned cattle or buffalo embryos were<br />
produced by fusion <strong>of</strong> the individual donor cells with enucleated cattle or buffalo oocytes, respectively. The reconstructed<br />
(cloned) embryos and in vitro matured oocytes without enucleation were parthenogenetically activated (PA) and cultured for<br />
7 days. Their developmental ability to the blastocyst stage was evaluated. The total number <strong>of</strong> trophectoderm (TE) and inner<br />
cell mass (ICM) cells and the ICM ratio in each blastocyst was determined by differential staining as an indicator <strong>of</strong> embryo<br />
quality. The fusion rate <strong>of</strong> CCs with enucleated oocytes was significantly lower than for those <strong>of</strong> other donor cell types both<br />
in cattle and buffalo. The rates <strong>of</strong> cleavage and development to the 8-cell, morula and blastocyst stages <strong>of</strong> cloned embryos<br />
derived from all donor cell types did not significantly differ within the same species. However, the cleavage rate <strong>of</strong> cloned<br />
cattle embryos derived from FFs was significantly higher than those <strong>of</strong> cattle PA and cloned buffalo embryos. The blastocyst<br />
rates <strong>of</strong> cloned cattle embryos, except for the ones derived from CCs, were significantly higher than those <strong>of</strong> cloned buffalo<br />
embryos. In buffalo, only cloned embryos derived from CCs showed a significantly higher blastocyst rate than that <strong>of</strong> PA<br />
embryos. In contrast, all the cloned cattle embryos showed significantly higher blastocyst rates than that <strong>of</strong> PA embryos.<br />
There was no difference in ICM ratio among any <strong>of</strong> the blastocysts derived from any <strong>of</strong> the donor cell types and PA embryos<br />
in both species. FFs, EFs, GCs and CCs had similar potentials to support development <strong>of</strong> cloned cattle and buffalo embryos<br />
to the blastocyst stage with the same quality. © <strong>2010</strong> by the Society for Reproduction and Development.<br />
No.1355<br />
Author (s) : Srisawasdi, P., Vanavanan, S., Charoenpanichkit, C., Kroll, M.<br />
Title : The authors' reply<br />
Source : American Journal <strong>of</strong> Clinical Pathology, Volume 134, Issue 3, September <strong>2010</strong>, Page 517<br />
Document Type : Letter<br />
Keywords : amino terminal pro brain natriuretic peptide, brain natriuretic peptide; glomerulus filtration rate, hemodialysis, human, kidney<br />
failure, kidney function, letter, priority journal<br />
<strong>Abstract</strong> : –<br />
No.1356<br />
Author (s) : Srisawasdi, P., Vanavanan, S., Charoenpanichkit, C., Kroll, M.H.<br />
Title : The effect <strong>of</strong> renal dysfunction on BNP, NT-proBNP, and their ratio<br />
Source : American Journal <strong>of</strong> Clinical Pathology, Volume 133, Issue 1, January <strong>2010</strong>, Pages 14-23<br />
Document Type : Article<br />
Keywords : B-natriuretic peptide; BNP; Estimated glomerular filtration rate; Heart disease; Left ventricular ejection fraction; N-terminal<br />
proBNP; Natriuretic peptide; New York Heart Association class; NT-proBNP; NT-proBNP/BNP ratio; Renal dysfunction<br />
<strong>Abstract</strong> : We examined the effect <strong>of</strong> renal dysfunction on B-natriuretic peptide (BNP), N-terminal (NT)-proBNP, and their molar ratio at<br />
varying severities <strong>of</strong> cardiac function in 94 Thai patients with chest pain (52 men; 32 women), also measuring creatinine and<br />
left ventricular ejection fraction (LVEF). Renal function was classified into 5 stages by estimated glomerular filtration rate. The<br />
molar NT-proBNP/BNP ratio was calculated. Cardiac status was classified by LVEF (normal, >50%; moderate, 35%-50%;<br />
severe, <35%). BNP, NT-proBNP, and their ratio corresponded to renal disease stage exponential (0.51, 1.05, and 0.54,<br />
respectively; correlation coefficients, ≥0.95). BNP and the ratio are affected less than NT-proBNP by renal dysfunction, starting<br />
in stage III; NT-proBNP expresses effects starting in stage II. NT-proBNP is more sensitive than BNP to renal disease stage. For<br />
log <strong>of</strong> geometric means vs stage <strong>of</strong> renal disease, the BNP slopes and correlation coefficients vary considerably (slopes, 0.036-<br />
0.531; r2, 0.017-0.99). The NT-proBNP slopes and regression coefficients vary considerably (slopes, 0.18-0.71; r2, 0.33-0.99). For<br />
the ratio, the slopes show low variation (0.148-0.337), r2 greater than 0.96, women differing from men (P = .012). The effect <strong>of</strong><br />
493
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
renal disease differs by gender. BNP and NT-proBNP increase by stage III for women but not for men. One must consider renal<br />
function, gender, and LVEF when using BNP or NT-proBNP as cardiac biomarkers. The ratio <strong>of</strong> the 2 peptides is the most<br />
consistent marker across LVEFs. © American Society for Clinical Pathology.<br />
No.1357<br />
Author (s) : Srisawasdi, P.; Chaichanajarernkul, U.; Teerakanjana, N.; Vanavanan, S.; Kroll, M.H.<br />
Title : Exogenous Interferences with Jaffe Creatinine Assays: Addition <strong>of</strong> Sodium Dodecyl Sulfate to Reagent Eliminates Bilirubin and<br />
Total Protein Interference with Jaffe Methods<br />
Source : JOURNAL OF CLINICAL LABORATORY ANALYSIS, Volume: 24, Issue: 3, Pages: 123-133, DOI: 10.1002/jcla.20350, Published: <strong>2010</strong><br />
Document Type : Article<br />
Keywords : kinetic Jaffe method; enzymatic method; analytical performance; Commercial; glomerular filtration rate<br />
<strong>Abstract</strong> : Background: The study evaluated the impact <strong>of</strong> interferences on the analytical specificity <strong>of</strong> three commercial and commonly<br />
used creatinine methods (two Jaffe and one enzymatic). Methods: Manufacturer creatinine methods plus modified methods<br />
were tested with the following interferences: spiking serum with bilirubin, albumin, glucose, hemoglobin and lipid, and patient<br />
sera with maximum concentrations <strong>of</strong> bilirubin, 1,090 mu mol/l and protein, 117.8 g/l. Results: Hemoglobin, 7.5 g/l and<br />
lipaemic with triglyceride concentration <strong>of</strong> 6.27 mmol/l, did not interfere with all assays. Glucose >33.3 mmol/l increased<br />
creatinine recovery for Dimension method. Samples spiked with bilirubin imparted a negative bias for Dimension and Architect<br />
methods but imparted a positive bias for Vitros assay. However, using patient sera, negative bias with bilirubin was found for<br />
all methods, from which Architect method gave the highest effect (R(2) = 0.861), followed by Vitros (R(2) = 0.239) and<br />
Dimension (R(2) = 0.163). Protein provided the positive bias for all creatinine measurements that increased with increasing<br />
concentration (R2 ranging from 0.104 to 0.182, P
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
preferred sequences in one strand <strong>of</strong> the DNA, assuming the aptamer invaded the duplex. The aptamer binding sites on the<br />
LTR were experimentally determined to be located preferentially at these sites near the termini <strong>of</strong> double-stranded target<br />
DNA, despite selection schemes that were designed to minimize preferences for termini. The results presented here show<br />
that aptamer RNAs can be selected in vitro that strand-invade at preferred DNA duplex sequences to form stable complexes.<br />
© <strong>2010</strong> The Author(s).<br />
No.1360<br />
Author (s) : Srisawat, R., Komalamisra, N., Eshita, Y., Zheng, M., Ono, K., Itoh, T.Q., Matsumoto, A., Petmitr, S., Rongsriyam, Y.<br />
Title : Point mutations in domain II <strong>of</strong> the voltage-gated sodium channel gene in deltamethrin-resistant aedes aegypti (diptera:<br />
culicidae)<br />
Source : Applied Entomology and Zoology, Volume 45, Issue 2, <strong>2010</strong>, Pages 275-282<br />
Document Type : Article<br />
Keywords : Aedes aegypti; Deltamethrin; Knockdown resistance; Point mutation; Voltage-gated sodium channel<br />
<strong>Abstract</strong> : The continuous usage <strong>of</strong> pyrethroids against insects has provoked the emergence <strong>of</strong> insecticide resistance that has become a<br />
major obstacle to disease vector control. The knockdown resistance (kdr) voltage-gated sodium channel gene is regarded as a<br />
key to understanding the mechanism <strong>of</strong> resistance to pyrethroids. The main purpose <strong>of</strong> this study is to identify point<br />
mutations in the sodium channel gene associated with deltamethrin resistance in Aedes aegypti. Two mutations in the IIS6<br />
domain <strong>of</strong> the channel, S989P and V1016G, were identified as possible candidates responsible for the emergence <strong>of</strong><br />
deltamethrin resistance in Ae. aegypti Khu Bua strain. As S989P and V1016G mutations are located within the IIS5-S6 loop and<br />
IIS6 near the ion filter and binding site, these mutations might enhance pyrethroid resistance. Allelic variation in the sodium<br />
channel gene is thought to be one <strong>of</strong> the principal molecular mechanisms regulating pyrethroid resistance in mosquitoes.<br />
No.1361<br />
Author (s) : Srisomsap, C.; Sawangareetrakul, P.; Subhasitanont, P.; Chokchaichamnankit, D.; Chiablaem, K.; Bhudhisawasdi, V.; Wongkham,<br />
S.; Svasti, J.<br />
Title : Proteomic Studies <strong>of</strong> Cholangiocarcinoma and Hepatocellular Carcinoma Cell Secretomes<br />
Source : JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY, Article Number: 437143, DOI: 10.1155/<strong>2010</strong>/437143, Published: <strong>2010</strong><br />
Document Type : Article<br />
Keywords : CANCER-CELLS; CATHEPSIN-D; NASOPHARYNGEAL CARCINOMA; PROTEIN; EXPRESSION; LINES; ANGIOGENESIS; GELATINASE;<br />
APOPTOSIS; ANNEXINS<br />
<strong>Abstract</strong> : Cholangiocarcinoma (CCA) and hepatocellular carcinoma (HCC) occur with relatively high incidence in Thailand. The<br />
secretome, proteins secreted from cancer cells, are potentially useful as biomarkers <strong>of</strong> the diseases. Proteomic analysis was<br />
performed on the secreted proteins <strong>of</strong> cholangiocarcinoma (HuCCA-1) and hepatocellular carcinoma (HCC-S102, HepG2, SK-<br />
Hep-1, and Alexander) cell lines. The secretomes <strong>of</strong> the five cancer cell lines were analyzed by SDS-PAGE combined with<br />
LC/MS/MS. Sixty-eight proteins were found to be expressed only in HuCCA-1. Examples include neutrophil gelatinaseassociated<br />
lipocalin (lipocalin 2), laminin 5 beta 3, cathepsin D precursor, desmoplakin, annexin IV variant, and annexin A5.<br />
Immunoblotting was used to confirm the presence <strong>of</strong> lipocalin 2 in conditioned media and cell lysate <strong>of</strong> 5 cell lines. The<br />
results showed that lipocalin 2 was a secreted protein which is expressed only in the conditioned media <strong>of</strong> the<br />
cholangiocarcinoma cell line. Study <strong>of</strong> lipocalin 2 expression in different types <strong>of</strong> cancer and normal tissues from<br />
cholangiocarcinoma patients showed that lipocalin 2 was expressed only in the cancer tissues. We suggest that lipocalin 2<br />
may be a potential biomarker for cholangiocarcinoma.<br />
No.1362<br />
Author (s) : Srisont, S., Chirachariyavej, T., Peonim, A.V.M.V.<br />
Title : Application <strong>of</strong> the multiple drugs immunoassay test for rapid detection <strong>of</strong> drug abuse in postmortem urine<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 11, November <strong>2010</strong>, Pages 1301-1309<br />
Document Type : Article<br />
Keywords : Barbiturates; Benzodiazepines; Cocaine; Drugs <strong>of</strong> abuse; Immunoassay test; MDMA; Medico legal<br />
autopsy; Methamphetamine; Opiates; Postmortem urine; REMEDI<br />
<strong>Abstract</strong> : Objective: The ADVANCED QUALITY One Step Multi-Drug Screen test is a new urine on-site immunoassay test that is<br />
designed to detect multiple drugs <strong>of</strong> abuse in one time (barbiturates, benzodiazepines, cocaine,<br />
methylenedioxymethamphetamine (MDMA), methamphetamine, and opiates group). Thus, the present research was done to<br />
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evaluate the diagnostic performance <strong>of</strong> this test. Material and Method: Urine samples obtained from corpses subjected to<br />
medicolegal autopsy at the forensic unit in Ramathibodi Hospital between October 2007 and March 2009 were used for the<br />
present study. The diagnostic performance <strong>of</strong> this immunoassay test was determined by using the results <strong>of</strong> the rapid<br />
emergency drug identification high sensitivity (REMEDi HS) system as the gold standard. Results: Two hundred forty six urine<br />
samples were used in the present study. The sensitivity with their 95% confidence interval <strong>of</strong> cocaine, opiates,<br />
methamphetamine, and benzodiazepines assay was 100% (100-100%) each. The specificity with their 95% confidence interval<br />
<strong>of</strong> these was 100% (100-100%), 98% (96.75-99.94%), 95% (91.70-97.38%), and 93% (89.89-96.24%), respectively. The MDMA<br />
and barbiturates were not evaluated because there was no true positive result. Conclusion: The ADVANCED QUALITY One<br />
Step Multi-Drug Screen test is reliable for drugs <strong>of</strong> abuse screening in postmortem urine.<br />
No.1363<br />
Author (s) : Srisuma, S., Bhattacharya, S., Simon, D.M., Solleti, S.K., Tyagi, S., Starcher, B., Mariani, T.J.<br />
Title : Fibroblast growth factor receptors control epithelial-mesenchymal interactions necessary for alveolar elastogenesis<br />
Source : American Journal <strong>of</strong> Respiratory and Critical Care Medicine, Volume 181, Issue 8, 15 April <strong>2010</strong>, Pages 838-850<br />
Document Type : Article<br />
Keywords : Alveogenesis; Fibroblast growth factor receptor; Insulin-like growth factor-1; Lung development; Microarray<br />
<strong>Abstract</strong> : Rationale: The mechanisms contributing to alveolar formation are poorly understood. A better understanding <strong>of</strong> these<br />
processes will improve efforts to ameliorate lung disease <strong>of</strong> the newborn and promote alveolar repair in the adult. Previous<br />
studies have identified impaired alveogenesis in mice bearing compound mutations <strong>of</strong> fibroblast growth factor (FGF) receptors<br />
(FGFRs) 3 and 4, indicating that these receptors cooperatively promote postnatal alveolar formation. Objectives: To determine<br />
the molecular and cellular mechanisms <strong>of</strong> FGF-mediated alveolar formation. Methods: Compound FGFR3/FGFR4-deficient<br />
mice were assessed for temporal changes in lung growth, airspace morphometry, and genome-wide expression. Observed<br />
gene expression changes were validated using quantitative real-time RT-PCR, tissue biochemistry, histochemistry, and ELISA.<br />
Autocrine and paracrine regulatory mechanisms were investigated using isolated lung mesenchymal cells and type II<br />
pneumocytes. Measurements and Main Results: Quantitative analysis <strong>of</strong> airspace ontogeny confirmed a failure <strong>of</strong> secondary<br />
crest elongation in compound mutant mice. Genome-wide expression pr<strong>of</strong>iling identified molecular alterations in these mice<br />
involving aberrant expression <strong>of</strong> numerous extracellular matrix molecules. Biochemical and histochemical analysis confirmed<br />
changes in elastic fiber gene expression resulted in temporal increases in elastin deposition with the loss <strong>of</strong> typical spatial<br />
restriction. No abnormalities in elastic fiber gene expression were observed in isolated mesenchymal cells, indicating that<br />
abnormal elastogenesis in compound mutant mice is not cell autonomous. Increased expression <strong>of</strong> paracrine factors,<br />
including insulin-like growth factor21, in freshly-isolated type II pneumocytes indicated that these cells contribute to the<br />
observed pathology. Conclusions: Epithelial/mesenchymal signaling mechanisms appear to contribute to FGFR-dependent<br />
alveolar elastogenesis and proper airspace formation.<br />
No.1364<br />
Author (s) : Srisuphanunt, M., Karanis, P., Charoenca, N., Boonkhao, N., Ongerth, J.E.<br />
Title : Cryptosporidium and Giardia detection in environmental waters <strong>of</strong> southwest coastal areas <strong>of</strong> Thailand<br />
Source : Parasitology Research, Volume 106, Issue 6, May <strong>2010</strong>, Pages 1299-1306<br />
Document Type : Article<br />
Keywords : drinking water, lake water, river water, sea water, tap water; article, coastal waters, controlled<br />
study, Cryptosporidium, Giardia, immun<strong>of</strong>luorescence test, nonhuman, oocyst, parasite identification, priority journal, public<br />
health, Thailand, turbidity, water analysis, water contamination, water quality, water sampling, water supply;<br />
Cryptosporidium, Giardia, Humans, Microscopy, Fluorescence, Microscopy, Interference, Seawater, Thailand;<br />
Cryptosporidium, Giardia, Protozoa<br />
<strong>Abstract</strong> : The aim <strong>of</strong> this study was to investigate water samples collected in coastal areas <strong>of</strong> Southern Thailand in the years <strong>of</strong> 2005<br />
and 2008 for their contamination by the protozoan parasites Cryptosporidium and Giardia. One hundred eighteen water<br />
samples <strong>of</strong> different origin were collected from six Tsunami affected southern provinces <strong>of</strong> Thailand in early 2005, and they<br />
have been analyzed using standardized methodology. Fifteen out <strong>of</strong> 118 samples (12.7%) were positive for Cryptosporidium<br />
spp. and nine (7.6%) positive for Giardia spp. Additional 42 samples from two same areas were examined 3 years later, in the<br />
early 2008. Five out <strong>of</strong> 42 (11.9%) samples were positive for Cryptosporidium spp., and three out <strong>of</strong> 42 (7.1%) were positive<br />
for Giardia spp.. Both protozoans were found in reservoir, river/canal, and pond waters. It appears no significant differences<br />
(p
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
the investigated areas and samples in the second period was significantly less than in the first period. This is the first<br />
description on Cryptosporidium and Giardia (oo)cysts in water sources <strong>of</strong> Thailand, and it suggests the need for water quality<br />
control in the interest <strong>of</strong> public health safety. © <strong>2010</strong> Springer-Verlag.<br />
No.1365<br />
Author (s) : Srisuwatanasagul, S., Manee-In, S., Kunkitti, P., Srisuwatanasagul, K.<br />
Title : Localization <strong>of</strong> oestrogen receptors alpha (ERα) and beta (ERβ) within various buffalo ovarian cell types<br />
Source : Thai Journal <strong>of</strong> Veterinary Medicine, Volume 40, Issue 1, March <strong>2010</strong>, Page 106<br />
Document Type : Article<br />
Keywords : Buffalo; ERα; ERβ; Ovary<br />
<strong>Abstract</strong> : –<br />
No.1366<br />
Author (s) : Sritunyalucksana, K., Srisala, J., Wangnai, W., Flegel, T.W.<br />
Title : Yellow head virus (YHV) transmission risk from commodity shrimp is reduced to negligible levels by normal processing<br />
Source : Aquaculture, Volume 300, Issue 1-4, 27 February <strong>2010</strong>, Pages 32-36<br />
Document Type : Article<br />
Keywords : Commodity shrimp; Infectivity; Processing; Shrimp; Transmission; Yellow head virus<br />
<strong>Abstract</strong> : The aim <strong>of</strong> this study was to examine whether shrimp yellow head virus (YHV) from processed shrimp tissue infected at the<br />
pre-patent disease level could be transmitted to naïve shrimp in a laboratory setting. In a preliminary test, 120 YHV-free<br />
shrimp were injected intramuscularly with a virulent YHV stock to yield 5 × 105 (pre-patent disease level) and 2500 (carrier<br />
level) viral copies/g shrimp tissue (60 shrimp each dose). These are possible infection levels for grossly normal shrimp from<br />
normal harvest ponds (i.e., not shrimp from disease outbreak ponds). These yielded 1-step and 2-step positive (nested) RT-<br />
PCR reactions, respectively, in pleopods at 6 h post-injection <strong>of</strong> the viral stock. After being subjected to standard industrial<br />
processing conditions, only fresh frozen whole or peeled shrimp injected with pre-patent dose gave positive RT-PCR test<br />
results for YHV. None <strong>of</strong> the naïve shrimp exposed to the chopped processed products for 24 h and then reared on a<br />
standard diet for 14 days showed any significant mortality or gave any positive test results for YHV using nested RT-PCR<br />
assays. Based on these preliminary test results, a large-scale test was carried out using only the high, pre-patent injection<br />
dose with 1000 fresh frozen whole shrimp. The negative control consisted <strong>of</strong> 1000 buffer-injected shrimp. A random sample<br />
<strong>of</strong> 60 shrimp from the YHV-injected group after processing, revealed 57 positive for YHV by 1-step RT-PCR assay. Of the 3<br />
remaining, 2 were positive and 1 negative by nested RT-PCR assay. All 60 shrimp from the buffer-injected, control group were<br />
negative for YHV by nested RT-PCR assay. Exposure <strong>of</strong> these whole shrimp to naïve shrimp resulted in no significant mortality<br />
and no positive RT-PCR test results for YHV by nested RT-PCR assay in the exposed naïve shrimp. Our results showed that<br />
grossly normal whole shrimp processed by chilling and freezing would present negligible YHV disease transmission risks, even<br />
if they were 1-step RT-PCR positive for YHV. Thus, shrimp subjected to any additional processing steps such as peeling or<br />
cooking should present even lower transmission risks. © <strong>2010</strong> Elsevier B.V. All rights reserved.<br />
No.1367<br />
Author (s) : Srivanich, N., Ngarmukos, C., Sungkanuparph, S.<br />
Title : Prevalence <strong>of</strong> and risk factors for pre-diabetes in HIV-1-infected patients in Bangkok, Thailand<br />
Source : Journal <strong>of</strong> the <strong>International</strong> Association <strong>of</strong> Physicians in AIDS Care, Volume 9, Issue 6, November <strong>2010</strong>, Pages 358-361<br />
Document Type : Article<br />
Keywords : diabetes; HIV-1; nevirapine; pre-diabetes; risk factor<br />
<strong>Abstract</strong> : Pre-diabetes substantially increases the risk <strong>of</strong> developing macrovascular complication and progression to diabetes. This study<br />
aimed to determine the prevalence <strong>of</strong> and risk factors for pre-diabetes in HIV-1-infected patients. A cross-sectional study was<br />
conducted in HIV-1-infected patients who visited the infectious diseases clinic in a university hospital. Fasting plasma glucose<br />
(FPG) was performed. There were 149 patients, mean age 42.2 years, and 65.1% were males. Median CD4 count was 434<br />
cells/mm3. In total, 92% have received antiretroviral therapy (ART), with a median duration <strong>of</strong> 0.8 years. The prevalence <strong>of</strong><br />
pre-diabetes was 27.5%. From multivariate analysis, body weight ([BW] per 5 kg increase, odds ratio [OR] = 1.241; 95%<br />
confidence interval [CI], 1.014-1.518; P =.036) and, tentatively, male gender (OR = 2.906; 95% CI, 0.941-8.976; P =.064) were<br />
risk factors for pre-diabetes. Nevirapine (NVP) use (OR = 0.383; 95% CI, 0.161-0.910; P =.030) was a protective factor for pre-<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
diabetes. Pre-diabetes is common in HIV-1-infected patients receiving ART. Screening for pre-diabetes and active management<br />
should be performed in patients with risk factors. © The Author(s) <strong>2010</strong>.<br />
No.1368<br />
Author (s) : Sriwattanarothai, N., Steinke, D., Ruenwongsa, P., Hanner, R., Panijpan, B.<br />
Title : Molecular and morphological evidence supports the species status <strong>of</strong> the Mahachai fighter Betta sp. Mahachai and reveals<br />
new species <strong>of</strong> Betta from Thailand<br />
Source : Journal <strong>of</strong> Fish Biology, Volume 77, Issue 2, August <strong>2010</strong>, Pages 414-424<br />
Document Type : Article<br />
Keywords : 16S rRNA; Betta smaragdina var.; Betta sp. Mahachai; Betta splendens complex; Cytochrome c oxidase subunit 1; DNA<br />
barcoding<br />
<strong>Abstract</strong> : Two regions <strong>of</strong> mitochondrial (mt) DNA, cytochrome c oxidase subunit 1 (COI) and 16S rRNA, were sequenced in nine species<br />
<strong>of</strong> Betta from Thailand and Indonesia. Most species showed little intraspecific COI variation (adjusted mean = 0.48%) including<br />
the putative species Betta sp. Mahachai, but one species (Betta smaragdina) included three lineages showing much greater<br />
divergence (7.03-13.48%) that probably represent overlooked species. These findings were confirmed by maximum likelihood<br />
analysis and Bayesian inference, which revealed well-supported corresponding monophyletic clades. Based on these results<br />
and morphological differences, the putative species Betta sp. Mahachai from central Thailand is a species distinct from other<br />
members <strong>of</strong> the B. splendens group and represents a new and hitherto undescribed species. Furthermore, this study also<br />
demonstrated the probable existence <strong>of</strong> two overlooked Betta species found in the Khorat plateau basin, illustrating the<br />
utility <strong>of</strong> mitochondrial genetic markers in the revelation <strong>of</strong> overlooked diversity. © <strong>2010</strong> Institute for Innovative Learning,<br />
<strong>Mahidol</strong> <strong>University</strong>. Journal compilation © <strong>2010</strong> The Fisheries Society <strong>of</strong> the British Isles.<br />
No.1369<br />
Author (s) : Sriwilaijaroen, N., Kondo, S., Nanthasri, P., Auparakkitanon, S., Suzuki, Y., Wilairat, P.<br />
Title : Antiplasmodial effects <strong>of</strong> Brucea javanica (L.) merr. and Eurycoma longifolia jack extracts and their combination with<br />
chloroquine and quinine on Plasmodium falciparum in culture<br />
Source : Tropical Medicine and Health, Volume 38, Issue 2, <strong>2010</strong>, Pages 61-68<br />
Document Type : Article<br />
Keywords : Antimalarial activity; Brucea javanica (L.) merr.; Drug combination; Eurycoma longifolia jack; Plasmodium<br />
falciparum; Traditional medicine<br />
<strong>Abstract</strong> : Fruits <strong>of</strong> Brucea javanica (L.) Merr. ("Ratchadad" in Thai) and roots <strong>of</strong> Eurycoma longifolia Jack (Pla-laipeag in Thai) are used as<br />
traditional medicines for the treatment <strong>of</strong> malarial fever. Ethanol, methanol, ethyl acetate, ethyl alcohol and aqueous<br />
extracts were tested against the multidrug-resistant Plasmodium falciparum strain K1. Ethanol and methanol-ethanol extracts,<br />
together with methanol residue, from fruits <strong>of</strong> B. javanica (L.) Merr. showed the highest antiplasmodial activities with IC50<br />
values <strong>of</strong> 0.5 ± 0.3, 0.3 ± 0.1 and 0.3 ± 0.05 μg/mL, respectively, comparable to the IC50 values <strong>of</strong> chloroquine (0.17 ± 0.02<br />
μg/mL) and quinine (0.3 ± 0.1 (μg/mL). Similarly, ethanol and methanol-ethanol extracts <strong>of</strong> roots <strong>of</strong> E. longifolia Jack showed<br />
higher activities than those <strong>of</strong> the other solvent extracts, but their activities were about 10-fold lower than those <strong>of</strong> extracts<br />
from B. javanica (L.) Merr. fruit. In drug combination tests, B. javanica (L.) Merr. and E. longifolia Jack extracts did not appear to<br />
antagonize antiplasmodial activity <strong>of</strong> chloroquine and quinine. Not only well-known quassinoid gly-cosides but also<br />
coumarins and flavonoids identified by thin-layer chromatography in ethanol and methanol-ethanol extracts and in methanol<br />
residue <strong>of</strong> B. javanica (L.) Merr fruit and E. longifolia roots may be responsible for the antimalarial activity. Taken together, our<br />
extraction conditions provided extracts containing novel active compounds that did not antagonize the inhibitory effects <strong>of</strong><br />
the two widely used antimalarials. This finding could lend support to the future discovery <strong>of</strong>active antimalaria compounds<br />
<strong>of</strong>Brucea javanica (L.) Merr. and Eurycoma longifolia Jack as drugs for the treatment <strong>of</strong> malaria that could be employed as<br />
drug combinations in order to delay the onset <strong>of</strong> parasite drug resistance. © <strong>2010</strong> by The Japanese Society <strong>of</strong> Tropical<br />
Medicine.<br />
No.1370<br />
Author (s) : Sroisiri, T., Boonyanit, T.<br />
Title : Inhibition <strong>of</strong> candida adhesion to denture acrylic by Boesenbergia pandurata<br />
Source : Asian Pacific Journal <strong>of</strong> Tropical Medicine, Volume 3, Issue 4, April <strong>2010</strong>, Pages 272-275<br />
Document Type : Article<br />
498
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : Adhesion; Boesenbergia pandurata; Candida; Denture<br />
<strong>Abstract</strong> : Objective: To investigate effect <strong>of</strong> Boesenbergia pandurata (B. pandurata) rhizome extract on adhesion <strong>of</strong> Candida albicans (C.<br />
albicans) to acrylic surface. Methods: Transparent acrylic strips were prepared and divided into three groups with<br />
pretreatment by extract solution <strong>of</strong> B. pandurata rhizome at concentration <strong>of</strong> 25, 50 and 100 mg/mL, respectively. After<br />
washing, the strips were then inoculated with two strains <strong>of</strong> C. albicans (ATCC13803 and the clinical isolate) (107cells/mL).<br />
Normal saline solution and 0.2% chlorhexidine gluconate were used as negative and positive controls, respectively. Stained<br />
the strips with modified Gram stain without counterstain. Adherent yeast cells were direct counted under microscope<br />
(Olympus-CX31, Japan) in 20 randomly selected fields on each strip. The statistical significance was calculated by Kruskal-<br />
Wallis and Mann-Whitney non-parametric tests at a significance level <strong>of</strong> P < 0.05. Results: Pretreatment with B. pandurata<br />
extract significantly reduced the adhesion <strong>of</strong> both strains <strong>of</strong> C. albicans to acrylic surfaces in a dose dependent manner.<br />
Conclusions: This observation indicates that B. pandurata extract has an inhibitory effect on the ability <strong>of</strong> C. albicans to<br />
adhere to denture acrylic and could be employed as an antifungal agent for preventing denture stomatitis. © <strong>2010</strong> Hainan<br />
Medical College.<br />
No.1371<br />
Author (s) : Srosiri, T., Sopee, P., Boonyanit, T.<br />
Title : Effect <strong>of</strong> lead on IL-8 production and cell proliferation in human oral keratinocytes<br />
Source : Asian Pacific Journal <strong>of</strong> Tropical Medicine, Volume 3, Issue 6, June <strong>2010</strong>, Pages 475-478<br />
Document Type : Article<br />
Keywords : Cell proliferation; Interleukin 8; Lead; Oral keratinocytes; Ulcerative stomatitis<br />
<strong>Abstract</strong> : Objective: To investigate the effect <strong>of</strong> lead on the production <strong>of</strong> IL-8 and cell proliferation in normal human oral<br />
keratinocytes (NHKs). Methods: NHKs were prepared as outgrowths from normal human buccal mucosa. The cells were<br />
treated with three concentrations <strong>of</strong> lead glutamate (4.5×10-5M, 4.5×10-6M and 4.5×10-7M). NHKs grown in glutamic acid<br />
were used as control. The amounts <strong>of</strong> IL-8 secreted in the culture supernatants were evaluated at 12 and 24 h using enzymelinked<br />
immunospecific assay (ELISA). Cell proliferation was determined by the MTT colorimetric assay. Three cultures were<br />
used for each experiment, and three independent experiments were performed. Analysis <strong>of</strong> variance and Duncan's multiple<br />
range tests were used for statistical analysis. Results: An elevation <strong>of</strong> IL-8 in culture supernatants <strong>of</strong> NHKs treated with lead at<br />
all concentrations at 12 and 24 h after exposure in a dose-dependent manner was revealed. A significant increase in cell<br />
numbers was observed only at 24 h exposed to 4.5×10-5M lead glutamate. Conclusions: The capacity <strong>of</strong> NHKs, to secrete IL-<br />
8, enhanced by lead glutamate, is demonstrated here. Induction <strong>of</strong> cell proliferation is revealed only after exposure to high<br />
lead concentration. The elevation <strong>of</strong> secreted IL-8 is a probable initial sign for the acute inflammatory response and may be<br />
involved in the pathogenesis <strong>of</strong> lead stomatitis. © <strong>2010</strong> Hainan Medical College.<br />
No.1372<br />
Author (s) : Sroyraya, M., Chotwiwatthanakun, C., Stewart, M.J., Soonklang, N., Kornthong, N., Phoungpetchara, I., Hanna, P.J., Sobhon, P.<br />
Title : Bilateral eyestalk ablation <strong>of</strong> the blue swimmer crab, Portunus pelagicus, produces hypertrophy <strong>of</strong> the androgenic gland and<br />
an increase <strong>of</strong> cells producing insulin-like androgenic gland hormone<br />
Source : Tissue and Cell, Volume 42, Issue 5, October <strong>2010</strong>, Pages 293-300<br />
Document Type : Article<br />
Keywords : Androgenic gland; Eyestalk ablation; Hypertrophy; Insulin-like androgenic gland hormone; Portunus pelagicus<br />
<strong>Abstract</strong> : The androgenic glands (AG) <strong>of</strong> male decapod crustaceans produce insulin-like androgenic gland (IAG) hormone that controls<br />
male sex differentiation, growth and behavior. Functions <strong>of</strong> the AG are inhibited by gonad-inhibiting hormone originating from<br />
X-organ-sinus gland complex in the eyestalk. The AG, and its interaction with the eyestalk, had not been studied in the blue<br />
swimmer crab, Portunus pelagicus, so we investigated the AG structure, and then changes <strong>of</strong> the AG and IAG-producing cells<br />
following eyestalk ablation. The AG <strong>of</strong> P. pelagicus is a small endrocrine organ ensheathed in a connective tissue and<br />
attached to the distal part <strong>of</strong> spermatic duct and ejaculatory bulb. The gland is composed <strong>of</strong> several lobules, each containing<br />
two major cell types. Type I cells are located near the periphery <strong>of</strong> each lobule, and distinguished as small globular cells <strong>of</strong><br />
5-7 μm in diameter, with nuclei containing mostly heterochromatin. Type II cells are 13-15 μm in diameter, with nuclei<br />
containing mostly euchromatin and prominent nucleoli. Both cell types were immunoreactive with anti-IAG. Following<br />
bilateral eyestalk ablation, the AG underwent hypertrophy, and at day 8 had increased approximately 3-fold in size. The<br />
percentage <strong>of</strong> type I cells had increased more than twice compared with controls, while type II cells showed a corresponding<br />
decrease. © <strong>2010</strong> Elsevier Ltd.<br />
499
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.1373<br />
Author (s) : Sroyraya, M., Goto-Inoue, N., Zaima, N., Hayasaka, T., Chansela, P., Tanasawet, S., Shrivas, K., Sobhon, P., Setou, M.<br />
Title : Visualization <strong>of</strong> biomolecules in the eyestalk <strong>of</strong> the blue swimming crab, Portunus pelagicus, by imaging mass spectrometry<br />
using the atmospheric-pressure mass microscope<br />
Source : Surface and Interface Analysis, Volume 42, Issue 10-11, October <strong>2010</strong>, Pages 1589-1592<br />
Document Type : Conference Paper<br />
Keywords : Atmospheric-pressure mass microscope; Eye; Eyestalk; Imaging mass spectrometry; Lipids; Metabolites; Portunus pelagicus<br />
<strong>Abstract</strong> : We have developed an imaging mass spectrometer-the atmospheric-pressure mass microscope-with high spatial resolution,<br />
which is composed <strong>of</strong> an atmospheric pressure ion-source chamber for matrix-assisted laser desorption/ionization (AP-MALDI)<br />
and a quadrupole ion trap time-<strong>of</strong>-flight (QIT-TOF) mass spectrometer. The production <strong>of</strong> 2-dimensional mass spectrum<br />
intensity by the atmospheric-pressure mass microscope with a very fine scan pitch enables us to visualize the distribution <strong>of</strong><br />
lipids and their metabolites in frozen sections without any specific probes or labels. In this study, we applied AP-MALDI-QIT-<br />
TOF imaging mass spectrometry to investigate the distribution <strong>of</strong> lipids in the compound eye-associated eyestalk <strong>of</strong> the blue<br />
swimming crab, Portunus pelagicus. The eyestalk <strong>of</strong> crustaceans responds to many signaling substances, and most <strong>of</strong> these<br />
substances are involved in lipid metabolism. The merged ion image <strong>of</strong> five significant biomolecules clearly showed seven<br />
separate zones in the eye and eyestalk. The results indicate that the lipid components <strong>of</strong> each cell layer <strong>of</strong> the compound<br />
eye are different. This study provides information regarding lipid compositions and locations on real tissue that correlate with<br />
function. Imaging mass spectrometry (IMS) with high spatial resolution could thus be a powerful tool for imaging and<br />
measurement <strong>of</strong> various samples for a first molecular screening. © <strong>2010</strong> John Wiley & Sons, Ltd.<br />
No.1374<br />
Author (s) : Stepniewska, K., Ashley, E., Lee, S.J., Anstey, N., Barnes, K.I., Binh, T.Q., D'Alessandro, U., Day, N.P.J., De Vries, P.J., Dorsey, G.,<br />
Guthmann, J.-P., Mayxay, M., Newton, P.N., Olliaro, P., Osorio, L., Price, R.N., Rowland, M., Smithuis, F., Taylor, W.R.J., Nosten,<br />
F., White, N.J.<br />
Title : In vivo parasitological measures <strong>of</strong> artemisinin susceptibility<br />
Source : Journal <strong>of</strong> Infectious Diseases, Volume 201, Issue 4, 15 February <strong>2010</strong>, Pages 570-579<br />
Document Type : Article<br />
Keywords : amodiaquine, antibiotic agent, artemether, artemisinin, artemisinin derivative, artesunate, atovaquone plus<br />
proguanil, benflumetol, chloroquine, clindamycin, dihydroartemisinin, doxycycline, fansidar, mefloquine, piperaquine, primaqu<br />
ine, quinine, tetracycline, trimethoprim; adolescent, article, blood smear, child, drug dose regimen, drug sensitivity, drug<br />
treatment failure, hospital admission, human, in vivo study, major clinical study, malaria falciparum, parasitology, predictor<br />
variable, preschool child, priority journal, school child, screening, treatment duration; Adolescent, Adult, Aged, Aged, 80 and<br />
over, Antimalarials, Artemisinins, Child, Child, Preschool, Drug Resistance, Endemic Diseases, Humans, Infant, Kaplan-Meiers<br />
Estimate, Malaria, Falciparum, Middle Aged, Parasitemia, Plasmodium falciparum, Recurrence<br />
<strong>Abstract</strong> : Parasite clearance data from 18,699 patients with falciparum malaria treated with an artemisinin derivative in areas <strong>of</strong> low (n<br />
= 14,539), moderate (n = 2077), and high (n = 2083) levels <strong>of</strong> malaria transmission across the world were analyzed to<br />
determine the factors that affect clearance rates and identify a simple in vivo screening measure for artemisinin resistance.<br />
The main factor affecting parasite clearance time was parasite density on admission. Clearance rates were faster in hightransmission<br />
settings and with more effective partner drugs in artemisinin-based combination treatments (ACTs). The result <strong>of</strong><br />
the malaria blood smear on day 3 (72 h) was a good predictor <strong>of</strong> subsequent treatment failure and provides a simple<br />
screening measure for artemisinin resistance. Artemisinin resistance is highly unlikely if the proportion <strong>of</strong> patients with parasite<br />
densities <strong>of</strong>
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : Spermatogenesis in the blue swimming crab, Portunus pelagicus, is described by light and electron microscopy. The testis is<br />
composed <strong>of</strong> anterior (AT) and posterior (PT) lobes, that are partitioned into lobules by connective tissue trabecula, and<br />
further divided into zones (germinal, transformation and evacuation), each with various stages <strong>of</strong> cellular differentiation. The<br />
vas deferens is classified into three distinct regions: anterior (AVD), median (MVD), and posterior (PVD), on the presence <strong>of</strong><br />
spermatophores and two secretions, termed substance I and II. Based on the degree and patterns <strong>of</strong> heterochromatin,<br />
spermatogenesis is classified into 13 stages: two spermatogonia (SgA and SgB), six primary spermatocytes (leptotene,<br />
zygotene, pachytene, diplotene, diakinesis, and metaphase), a secondary spermatocyte (SSc), three spermatids (St 1-3), and a<br />
mature spermatozoon. Spermatid stages are differentiated by chromatin decondensation and the formation <strong>of</strong> an acrosomal<br />
complex, which is unique to brachyurans. Mature spermatozoa are aflagellated, and have a nuclear projection and a spherical<br />
acrosome. AUT-PAGE and Western blots show that, during chromatin decondensation, there is a reduction <strong>of</strong> most histones,<br />
with only small amounts <strong>of</strong> H2B and H3 remaining in mature spermatozoa. © <strong>2010</strong> Elsevier Ltd.<br />
No.1376<br />
Author (s) : Stokes, R.B.<br />
Title : Gustave Cotteau's posthumous 1897 monograph on Miocene Spatangus (Echinoidea) <strong>of</strong> France, the missing plate 13, and the<br />
death <strong>of</strong> Paleontologie francaise<br />
Source : ARCHIVES OF NATURAL HISTORY, Volume: 37, Issue: 2, Pages: 318-324, DOI: 10.3366/E0260954110002032, Published: OCT <strong>2010</strong><br />
Document Type : Article<br />
Keywords : Tertiary; history <strong>of</strong> publication<br />
<strong>Abstract</strong> : The first issue <strong>of</strong> Cotteau's monograph on the post-Eocene echinoids <strong>of</strong> France, destined to be his final contribution to<br />
Paleontologie francaise, had reached pro<strong>of</strong> stage when he died in 1894. A limited number <strong>of</strong> copies <strong>of</strong> this first part, which<br />
included twelve plates, was printed for private circulation. Subsequently the section <strong>of</strong> the genus Spatangus was published in<br />
Isere as a posthumous journal article with the same twelve plates. Plate 13, <strong>of</strong> Spatangus heinzi Gauthier, whilst called for in<br />
the text, was not included. This paper presents the missing plate 13 which was prepared separately in the Yonne and never<br />
previously published; it identifies errors which were not corrected in the published version, and differences between<br />
Cotteau's original pro<strong>of</strong> version and that which was published. It also briefly summarises the history <strong>of</strong> the publication <strong>of</strong> the<br />
echinoid volumes <strong>of</strong> Paleontologie francaise and the reluctance <strong>of</strong> the publisher, George Masson, to continue its production.<br />
No.1377<br />
Author (s) : Stow, N.W., Douglas, R., Tantilipikorn, P., Lacroix, J.S.<br />
Title : Superantigens<br />
Source : Otolaryngologic Clinics <strong>of</strong> North America, Volume 43, Issue 3, June <strong>2010</strong>, Pages 489-502<br />
Document Type : Review<br />
Keywords : Allergy; Enterotoxin; Rhinitis; Rhinosinusitis; Staphylococcus aureus; Superantigen<br />
<strong>Abstract</strong> : Superantigens (SAgs) are derived from diverse sources, including bacteria, viruses, and human hepatic tissue. SAgs initially<br />
cause lymphocyte activation but then result in clonal deletion and anergy, leading to immune tolerance. They can also act<br />
as superallergens by stimulating a broad spectrum <strong>of</strong> mast cells and basophils in patients with allergic conditions. The newly<br />
described staphylococcal SAg-like proteins subvert innate immune function by several mechanisms, which are distinct from<br />
SAgs' effects on lymphocytes and other acquired immune processes. There is mounting evidence to suggest that SAgs play a<br />
role in the pathophysiology <strong>of</strong> inflammatory airway disease. The pathophysiologic role <strong>of</strong> SAg-like proteins awaits clarification.<br />
© <strong>2010</strong> Elsevier Inc.<br />
No.1378<br />
Author (s) : Stringer, J.S.A., McConnell, M.S., Kiarie, J., Bolu, O., Anekthananon, T., Jariyasethpong, T., Potter, D., Mutsotso, W., Borkowf, C.B.,<br />
Mbori-Ngacha, D., Muiruri, P., Ong'ech, J.O., Zulu, I., Njobvu, L., Jetsawang, B., Pathak, S., Bulterys, M., Shaffer, N., Weidle, P.J.<br />
Title : Effectiveness <strong>of</strong> non-nucleoside reverse-transcriptase inhibitor-based antiretroviral therapy in women previously exposed to a<br />
single intrapartum dose <strong>of</strong> nevirapine: A multi-country, prospective cohort study<br />
Source : PLoS Medicine, Volume 7, Issue 2, February <strong>2010</strong>, Article number e1000233<br />
Document Type : Article<br />
Keywords : cotrimoxazole, efavirenz, lamivudine plus zidovudine, nevirapine, rifampicin, ritonavir, stavudine, zidovudine, RNA directed<br />
DNA polymerase inhibitor; adult, article, cohort analysis, drug efficacy, drug treatment failure, environmental<br />
exposure, female, highly active antiretroviral therapy, human, Human immunodeficiency virus<br />
501
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
infection, hypothesis, Kenya, major clinical study, observational study, Pneumocystis pneumonia, prospective study, risk<br />
assessment, single drug dose, Thailand, treatment outcome, tuberculosis, virus load, Zambia, pregnancy, vertical transmission;<br />
Adult, Cohort Studies, Female, HIV Infections, Humans, Infectious Disease Transmission,<br />
Vertical, Kenya, Nevirapine, Pregnancy, Prospective Studies, Reverse Transcriptase Inhibitors, Thailand, Treatment<br />
Outcome, Zambia<br />
<strong>Abstract</strong> : Background: Intrapartum and neonatal single-dose nevirapine (NVP) reduces the risk <strong>of</strong> mother-to-child HIV transmission but<br />
also induces viral resistance to non-nucleoside reverse transcriptase inhibitor (NNRTI) drugs. This drug resistance largely fades<br />
over time. We hypothesized that women with a prior single-dose NVP exposure would have no more than a 10% higher<br />
cumulative prevalence <strong>of</strong> failure <strong>of</strong> their NNRTI-containing antiretroviral therapy (ART) over the first 48 wk <strong>of</strong> therapy than<br />
would women without a prior exposure. Methods and Findings: We enrolled 355 NVP-exposed and 523 NVP-unexposed<br />
women at two sites in Zambia, one site in Kenya, and two sites in Thailand into a prospective, non-inferiority cohort study<br />
and followed them for 48 wk on ART. Those who died, discontinued NNRTI-containing ART, or had a plasma viral load ≥400<br />
copies/ml at either the 24 wk or 48 wk study visits and confirmed on repeat testing were characterized as having failed<br />
therapy. Overall, 114 <strong>of</strong> 355 NVP-exposed women (32.1%) and 132 <strong>of</strong> 523 NVP-unexposed women (25.2%) met criteria for<br />
treatment failure. The difference in failure rates between the exposure groups was 6.9% (95% confidence interval [CI] 0.8%-<br />
13.0%). The failure rates <strong>of</strong> women stratified by our predefined exposure interval categories were as follows: 47 <strong>of</strong> 116<br />
women in whom less than 6 mo elapsed between exposure and starting ART failed therapy (40%; p
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : Antioxidant; Cardiac function; High fat diet; Liver function; Ocimum sanctum L.<br />
<strong>Abstract</strong> : It has been reported that Ocimum sanctum L. (OS) leaves decrease serum lipid pr<strong>of</strong>ile in normal and diabetic animals. No<br />
experimental evidences support the anti-hyperlipidemic and antioxidative actions against hypercholesterolemia. Moreover the<br />
identity <strong>of</strong> the specific chemical ingredients in OS leaves responsible for these pharmacological effects are unknown. Since OS<br />
leaves are rich in essential oil (EO). Therefore the present study was conducted to investigate the anti-hyperlipidemic and<br />
antioxidative activities <strong>of</strong> EO extracted from OS leaves in rats fed with high cholesterol (HC) diet. EO was extracted by the<br />
hydrodistillation method and the chemical constituents were then identified by Gas Chromatography-Mass Spectrometry. The<br />
experiment was performed in Male Wistar rats fed with 2.5 g%(w/w) <strong>of</strong> cholesterol diet for seven weeks. During the last 3<br />
weeks, rats were daily fed with EO. The results showed that phenyl propanoid compounds including eugenol and methyl<br />
eugenol were the major constituents <strong>of</strong> EO. EO suppressed the high serum lipid pr<strong>of</strong>ile and atherogenic index as well as<br />
serum lactate dehydrogenase and creatine kinase MB subunit without significant effect on high serum levels <strong>of</strong> aspartate<br />
aminotransferase, alanine amino-transferase and alkaline phosphatase in rats fed with HC diet. In addition, EO was found to<br />
decrease the high levels <strong>of</strong> thiobarbituric acid reactive substances (TBARS), glutathione peroxidase (GPx) and superoxide<br />
dismutase (SOD) without impacting catalase (CAT) in the cardiac tissue while in the liver, it decreased high level <strong>of</strong> TBARS<br />
without significantly effecting GPx, SOD and CAT. Histopathological results confirmed that EO preserved the myocardial tissue.<br />
It can be concluded that EO extracted from OS leaves has lipid-lowering and antioxidative effects that protect the heart<br />
against hypercholesterolemia. Eugenol that is contained in EO likely contribute to these pharmacological effects.<br />
No.1381<br />
Author (s) : Suantai, S., Cholamjiak, W.<br />
Title : A hybrid method for a countable family <strong>of</strong> multivalued maps, equilibrium problems, and variational inequality problems<br />
Source : Discrete Dynamics in Nature and Society, Volume <strong>2010</strong>, <strong>2010</strong>, Article number 349158<br />
Document Type : Article<br />
Keywords : –<br />
<strong>Abstract</strong> : We introduce a new monotone hybrid iterative scheme for finding a common element <strong>of</strong> the set <strong>of</strong> common fixed points <strong>of</strong><br />
a countable family <strong>of</strong> nonexpansive multivalued maps, the set <strong>of</strong> solutions <strong>of</strong> variational inequality problem, and the set <strong>of</strong><br />
the solutions <strong>of</strong> the equilibrium problem in a Hilbert space. Strong convergence theorems <strong>of</strong> the purposed iteration are<br />
established. Copyright © <strong>2010</strong> W. Cholamjiak and S. Suantai.<br />
No.1382<br />
Author (s) : Suantai, S., Singthong, U.<br />
Title : A new general iterative method for a finite family <strong>of</strong> nonexpansive mappings in hilbert spaces<br />
Source : Fixed Point Theory and Applications, Volume <strong>2010</strong>, <strong>2010</strong>, Article number 262691<br />
Document Type : Article<br />
<strong>Abstract</strong> : We introduce a new general iterative method by using the K -mapping for finding a common fixed point <strong>of</strong> a finite family <strong>of</strong><br />
nonexpansive mappings in the framework <strong>of</strong> Hilbert spaces. A strong convergence theorem <strong>of</strong> the purposed iterative method<br />
is established under some certain control conditions. Our results improve and extend the results announced by many others.<br />
Copyright <strong>2010</strong> Urailuk Singthong and Suthep Suantai.<br />
No.1383<br />
Author (s) : Sucharitakul, J., Wongnate, T., Chaiyen, P.<br />
Title : Kinetic isotope effects on the noncovalent flavin mutant protein <strong>of</strong> pyranose 2-oxidase reveal insights into the flavin<br />
reduction mechanism<br />
Source : Biochemistry, Volume 49, Issue 17, 4 May <strong>2010</strong>, Pages 3753-3765<br />
Document Type : Article<br />
Keywords : Binding modes, C<strong>of</strong>actors, Covalent linkage, D-glucose, Flavin adenine dinucleotide, Gain insight, H-bonds, Halfreactions,<br />
Kinetic constant, Kinetic isotope effects, Kinetic mechanism, Kinetic simulation, Michaelis complex, Mutant<br />
proteins, Noncovalent, OH group, Primary isotope effect, Pyranose, Reaction mechanism, Reduction mechanisms, Solvent<br />
kinetic isotope effects, Steady-state kinetics, Transient kinetic data, Transient kinetics, Wild types, Wild-type enzymes; Binding<br />
energy, Carrier mobility, Enzymes, Isotopes, Molecular oxygen, Oxidation, Rate constants, Reaction intermediates; Glucose;<br />
1,2,3,4,5,6,6 delta7 dextro glucose, 2 delta dextro glucose, 3 delta dextro glucose, 8a n 3 histidyl flavin adenine<br />
dinucleotide, alanine, carbon, enzyme, flavine adenine nucleotide, flavine nucleotide, flavoprotein, glucose, glucose<br />
503
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
derivative, histidine, hydrogen peroxide, hydroxyl group, oxidoreductase, oxygen, pyranose 2 oxidase, solvent, unclassified<br />
drug; amino acid substitution, article, binding affinity, catalysis, chemical bond, complex formation, controlled study, cross<br />
linking, deprotonation, enzyme kinetics, enzyme specificity, isotope analysis, kinetic isotope effect, Michaelis<br />
constant, mutant, oxidation reduction reaction, priority journal, protein cleavage, proton transport, wild type; Amino Acid<br />
Substitution, Binding Sites, Carbohydrate Dehydrogenases, Catalysis, Catalytic Domain, Flavins, Glucose, Kinetics, Models,<br />
Molecular, Molecular Structure, Mutagenesis, Site-Directed, Mutant Proteins, Mutation, Oxidation-Reduction, Trametes;<br />
Trametes ochracea<br />
<strong>Abstract</strong> : Pyranose 2-oxidase (P2O) from Trametes multicolor contains a flavin adenine dinucleotide (FAD) c<strong>of</strong>actor covalently linked to<br />
the N3 atom <strong>of</strong> His167. The enzyme catalyzes the oxidation <strong>of</strong> aldopyranoses by molecular oxygen to generate 2-ketoaldoses<br />
and H2O2 as products. In this study, the transient kinetics and primary and solvent kinetic isotope effects <strong>of</strong> the<br />
mutant in which His167 has been replaced with Ala (H167A) were investigated, to elucidate the functional role <strong>of</strong> the 8a-N3histidyl<br />
FAD linkage and to gain insights into the reaction mechanism <strong>of</strong> P2O. The results indicate that the covalent linkage is<br />
mainly important for a reductive half-reaction in which the FAD c<strong>of</strong>actor is reduced by d-glucose, while it is not important for<br />
an oxidative half-reaction in which oxygen reacts with the reduced FAD to generate H2O2. d-Glucose binds to H167A via<br />
multiple binding modes before the formation <strong>of</strong> the active Michaelis complex, and the rate constant <strong>of</strong> flavin reduction<br />
decreases ∼22-fold compared to that <strong>of</strong> the wild-type enzyme. The reduction <strong>of</strong> H167A using d-glucose isotopes (2-d-dglucose,<br />
3-d-d-glucose, and 1,2,3,4,5,6,6-d7-d-glucose) as substrates indicates that the primary isotope effect results only from<br />
substitution at the C2 position, implying that H167A catalyzes the oxidation <strong>of</strong> d-glucose regiospecifically at this position. No<br />
solvent kinetic isotope effect was detected during the reductive half-reaction <strong>of</strong> the wild-type or H167A enzyme, implying<br />
that the deprotonation <strong>of</strong> the d-glucose C2-OH group may occur readily upon the binding to P2O and is not synchronized<br />
with the cleavage <strong>of</strong> the d-glucose C2′H bond. The mutation has no drastic effect on the oxidative half-reaction <strong>of</strong> P2O, as<br />
H167A is very similar to the wild-type enzyme with respect to the kinetic constants and the formation <strong>of</strong> the C4ahydroperoxyflavin<br />
intermediate. Kinetic mechanisms for both half-reactions <strong>of</strong> H167A were proposed on the basis <strong>of</strong> transient<br />
kinetic data and were verified by kinetic simulations and steady-state kinetic parameters. © <strong>2010</strong> American Chemical Society.<br />
No.1384<br />
Author (s) : Suebsin, C., Gerdsri, N.<br />
Title : Technology adoption: A case study <strong>of</strong> ERP implementation in one <strong>of</strong> healthcare organizations in Thailand<br />
Source : PICMET '10 - Portland <strong>International</strong> Center for Management <strong>of</strong> Engineering and Technology, Proceedings - Technology<br />
Management for Global Economic Growth, <strong>2010</strong>, Article number 5603424, Pages 447-454<br />
Document Type : Conference Paper<br />
Keywords : ERP implementation, Healthcare organizations, In-depth interviews, Private hospitals, Technology adoption, Thailand, Work<br />
process; Economics, Industrial management, Resource allocation, Technology; Enterprise resource planning<br />
<strong>Abstract</strong> : This paper addresses the factors that affect technology adoption within a healthcare organization. A case study <strong>of</strong> the<br />
adoption <strong>of</strong> Enterprise Resource Planning (ERP) is conducted through a series <strong>of</strong> in-depth interviews to explore the situation <strong>of</strong><br />
technology adoption in one <strong>of</strong> the leading private hospitals in Thailand. The five factors were derived from the study<br />
including routine job, user resistance, ERP capability, complexity, and change <strong>of</strong> work process. © <strong>2010</strong> IEEE.<br />
No.1385<br />
Author (s) : Suewattana, M., Singh, D.J.<br />
Title : Local dynamics and structure <strong>of</strong> pure and Ta substituted ( K1-x Nax ) NbO3 from first principles calculations<br />
Source : Physical Review B - Condensed Matter and Materials Physics, Volume 82, Issue 1, 28 July <strong>2010</strong>, Article number 014114<br />
Document Type : Article<br />
Keywords : –<br />
<strong>Abstract</strong> : The local structure and dynamics <strong>of</strong> piezoelectric K1-x Na xNbO3 perovskite solid solutions with and without partial Ta<br />
substitution at x=0.5 are investigated using first principles calculations for supercells. The results are analyzed locally using<br />
the dynamical pair distribution functions. The local structures for Ta-substituted material show smaller <strong>of</strong>f-centering <strong>of</strong> Ta<br />
compared to Nb. In addition, the dynamics <strong>of</strong> the relaxed structure indicate s<strong>of</strong>ter Nb force constants relative to Ta even<br />
though the Nb has shorter O nearest-neighbor distances than Ta. These results are discussed in relation to experimental<br />
measurements which show a decrease in Curie temperature and an increase in dielectric constant when Ta is partially<br />
substituted into K1-x NaxNbO3. © <strong>2010</strong> The American Physical Society.<br />
504
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.1386<br />
Author (s) : Sugiyama, M.; Kaneko, Y.; Boonchird, C.; Harashima, S.<br />
Title : Molecular breeding <strong>of</strong> super yeast as bioethanol producer with tolerance to high temperature and low pH<br />
Source : JOURNAL OF BIOTECHNOLOGY, Volume: 150, Supplement: 1, Pages: S363-S363, DOI: 10.1016/j.jbiotec.<strong>2010</strong>.09.425, Published:<br />
NOV <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1387<br />
Author (s) : Suh, D.H., Kim, J.W., Aziz, M.F., Devi, U.K., Ngan, H.Y.S., Nam, J.-H., Kim, S.C., Kato, T., Ryu, H.S., Fujii, S., Lee, Y.S., Kim, J.H., Kim,<br />
T.-J., Kim, Y.T., Wang, K.-L., Lee, T.S., Ushijima, K., Shin, S.-G., Chia, Y.N., Wilailak, S., Park, S.Y., Katabuchi, H., Kamura, T., Kang,<br />
S.-B.<br />
Title : Asian society <strong>of</strong> gynecologic oncology workshop <strong>2010</strong><br />
Source : Journal <strong>of</strong> Gynecologic Oncology, Volume 21, Issue 3, September <strong>2010</strong>, Pages 137-150<br />
Document Type : Conference Paper<br />
Keywords : ASGO; Cervical caner; Clinical trials; Endometrial cancer; Ovarian cancer; Workshop<br />
<strong>Abstract</strong> : This workshop was held on July 31-August 1, <strong>2010</strong> and was organized to promote the academic environment and to enhance<br />
the communication among Asian countries prior to the 2nd biennial meeting <strong>of</strong> Australian Society <strong>of</strong> Gynaecologic<br />
Oncologists (ASGO), which will be held on November 3-5, 2011. We summarized the whole contents presented at the<br />
workshop. Regarding cervical cancer screening in Asia, particularly in low resource settings, and an update on human<br />
papillomavirus (HPV) vaccination was described for prevention and radical surgery overview, fertility sparing and less radical<br />
surgery, nerve sparing radical surgery and primary chemoradiotherapy in locally advanced cervical cancer, were discussed for<br />
management. As to surgical techniques, nerve sparing radical hysterectomy, optimal staging in early ovarian cancer,<br />
laparoscopic radical hysterectomy, one-port surgery and robotic surgery were introduced. After three topics <strong>of</strong> endometrial<br />
cancer, laparoscopic surgery versus open surgery, role <strong>of</strong> lymphadenectomy and fertility sparing treatment, there was a<br />
special additional time for clinical trials in Asia. Finally, chemotherapy including neo-adjuvant chemotherapy, optimal surgical<br />
management, and the basis <strong>of</strong> targeted therapy in ovarian cancer were presented.<br />
No.1388<br />
Author (s) : Sujjanunt, J.; Prasansiang, C.; Siritho, S.; Anamnart, C.; Jitpratoom, P.; Prayoonwiwat, N.<br />
Title : Azathioprine in Thai multiple sclerosis patients<br />
Source : MULTIPLE SCLEROSIS, Volume: 16, Issue: 10, Pages: 1282-1282, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1389<br />
Author (s) : Sukeeyamanon, W., Sirsiapisith, T., Wasinrat, J.<br />
Title : Preoperative localization <strong>of</strong> adamkiewicz arteries and their origins by using MDCT angiography<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 12, December <strong>2010</strong>, Pages 1430-1436<br />
Document Type : Article<br />
Keywords : Adamkiewicz arteries; MDCT angiography; Preoperative evaluation image visualization<br />
<strong>Abstract</strong> : Objective: To evaluate the ability <strong>of</strong> thoraco-abdominal MDCT angiography to visualize Adamkiewicz arteries for preoperative<br />
planning in patients diagnosed with aortic disease. Material and Method: The present study retrospectively reviewed clinical<br />
data from 73 patients who underwent a thoracoabdominal 64-slice MDCT angiography. The Adamkiewicz artery was evaluated<br />
on multiplanar reformation images in each case. The visualization <strong>of</strong> the Adamkiewicz artery, level <strong>of</strong> origin, side <strong>of</strong> origin and<br />
continuation from an intercostal artery was investigated. Results: The Adamkiewicz arteries were visualized in 52 <strong>of</strong> the 73<br />
patients (71.2%), and the total number <strong>of</strong> the delineated Adamkiewicz arteries was 64. Two Adamkiewicz arteries were found<br />
in nine patients (17.3%). Four Adamkiewicz arteries were found in one patient (1.9%). Most <strong>of</strong> the delineated arteries arose<br />
from the T9-L2 levels (89.1%). A left side <strong>of</strong> origin was found in 41 <strong>of</strong> 64 arteries (64.1%), and a right side <strong>of</strong> origin was found<br />
in 23 <strong>of</strong> 64 arteries (35.9%). Only 12 <strong>of</strong> 64 delineated arteries (18.8%) showed continuity from their origins to the anterior<br />
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radiculomedullary artery. Conclusion: The preoperative detection rate <strong>of</strong> the Adamkiewicz artery with the routine technique<br />
<strong>of</strong> 64-slice MDCT angiography was 71.2%. The preoperative location <strong>of</strong> the Adamkiewicz artery may help to reduce the risk <strong>of</strong><br />
perioperative ischemic changes in the spinal cord.<br />
No.1390<br />
Author (s) : Suksai, P., Sangkachai, N., Chatsiriwech, J., Kanthasaewee, O., Sariya, L., Chaichoun, K.<br />
Title : Development <strong>of</strong> multiplex polymerase chain reaction for detection <strong>of</strong> feline hemotropic mycoplasma in blood and tissue<br />
specimens<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 6, November <strong>2010</strong>, Pages 1447-1453<br />
Document Type : Article<br />
Keywords : Feline; Hemoplasma; Hemotropic mycoplasma; Multiplex PCR<br />
<strong>Abstract</strong> : A multiplex polymerase chain reaction (PCR) was developed for the detection <strong>of</strong> feline hemotropic mycoplasmas which<br />
simultaneously differentiates infections <strong>of</strong> Mycoplasma haem<strong>of</strong>elis (Mhf), Candidatus Mycoplasma haemominutum (CMhm)<br />
and Candidatus Mycoplasma turicensis (CMtc) in feline blood and spleen. These organisms are responsible for the cause <strong>of</strong><br />
various pathogenicity <strong>of</strong> feline infectious anemia. These infections are difficult to be detected by microscopic examination,<br />
the most commonly used method for general laboratory diagnoses. Specific primers were designed by selected consensus<br />
16S rDNA sequences <strong>of</strong> three distinct species. The multiplex PCR assay developed in this study was sensitive and specific with<br />
detection limit 100 copies/μl DNA <strong>of</strong> Mhf and CMhm and 10 copies/μl DNA <strong>of</strong> CMtc. No amplicons could be amplified for<br />
other blood parasites or bacterial pathogens. This multiplex PCR will allow studies <strong>of</strong> pathogenicity and the monitoring <strong>of</strong><br />
clinical treatment.<br />
No.1391<br />
Author (s) : Suksamanapun N., Uiprasertkul M., Ruangtrakool R., Akaraviputh T.<br />
Title : Endoscopic treatment <strong>of</strong> a large colonic polyp as a cause <strong>of</strong> colocolonic intussusception in a child.<br />
Source : World J Gastrointest Endosc, <strong>2010</strong> July 16; 2(7): 268-270<br />
Document Type : Article<br />
Keywords : Colocolonic intussusception; Juvenile polyp; Endoscopic treatment; Large colonic polyp<br />
<strong>Abstract</strong> : Colocolonic intussusception is an uncommon cause <strong>of</strong> intestinal obstruction in children. The most common type is idiopathic<br />
ileocolic intussusception. However, pathologic lead points occur approximately in 5% <strong>of</strong> cases. In pediatric patients, Meckel’s<br />
diverticulum is the most common lead point, followed by polyps and duplication. We present a case <strong>of</strong> recurrent<br />
colocolonic intussusception which caused colonic obstruction in a 10-year-old boy. A barium enema revealed a large<br />
polypoid mass at the transverse colon. Colonoscopy showed a colonic polyp, 3.5 centimeters in diameter, which was<br />
successfully removed by endoscopic polypectomy.<br />
No.1392<br />
Author (s) : Suksiriworapong, J., Sripha, K., Junyaprasert, V.B.<br />
Title : Synthesis and characterization <strong>of</strong> bioactive molecules grafted on poly(e{open}-caprolactone) by " click" chemistry<br />
Source : Polymer, Volume 51, Issue 11, May <strong>2010</strong>, Pages 2286-2295<br />
Document Type : Article<br />
Keywords : Bioactive molecules; Click chemistry; Poly(e{open}-caprolactone)<br />
<strong>Abstract</strong> : A facile and efficient strategy to graft bioactive molecules (nicotinic acid, p-aminobenzoic acid, and phthaloyltryptophan) onto<br />
poly(e{open}-caprolactone) (P(e{open}CL)) was achieved by copper-catalyzed Huisgen's 1,3-dipolar cycloaddition known as<br />
click reaction. P(αCle{open}CL), with 10, 20, and 30% <strong>of</strong> α-chloro-e{open}-caprolactone (αCle{open}CL) units were<br />
copolymerized by ring opening polymerization using e{open}CL and αCle{open}CL as starting materials in the presence <strong>of</strong> 1,4butanediol<br />
and Sn(Oct)2. Subsequently, the chloride pendent was converted to azide followed by cycloaddition with<br />
terminal alkyne derivatives <strong>of</strong> the aforementioned bioactive molecules. The complete addition was accomplished at all<br />
ratios. The characteristic molecular features <strong>of</strong> these copolymers were evaluated by FTIR, NMR, and GPC. Thermal analysis<br />
data indicated that the grafted compounds led to polymorphic alteration and different pattern <strong>of</strong> thermal degradation<br />
depending on the molecular structure and the size <strong>of</strong> the grafted compounds. They are the basis for further development <strong>of</strong><br />
grafted copolymer as drug delivery carriers. © <strong>2010</strong>.<br />
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No.1393<br />
Author (s) : Suksrichavalit, T., Yoshimatsu, K., Prachayasittikul, V., Bülow, L., Ye, L.<br />
Title : "Clickable" affinity ligands for effective separation <strong>of</strong> glycoproteins<br />
Source : Journal <strong>of</strong> Chromatography A, Volume 1217, Issue 23, June <strong>2010</strong>, Pages 3635-3641<br />
Document Type : Article<br />
Keywords : Affinity separation; Boronic acid; Click chemistry; Glycoprotein<br />
<strong>Abstract</strong> : In this paper, we present a new modular approach to immobilize boronic acid ligands that can <strong>of</strong>fer effective separation <strong>of</strong><br />
glycoproteins. A new " clickable" boronic acid ligand was synthesized by introducing a terminal acetylene group into<br />
commercially available 3-aminophenyl boronic acid. The clickable ligand, 3-(prop-2-. ynyloxycarbonylamino). phenylboronic<br />
acid (2) could be easily coupled to azide-functionalized hydrophilic Sepharose using Cu(I)-catalyzed 1,3-dipolar cycloaddition<br />
reaction under mild condition. Compared to other boronic acid affinity gels, the new affinity gel displayed superior<br />
effectiveness in separating model glycoproteins (ovalbumin and RNase B) from closely related bovine serum albumin and<br />
RNase A in the presence <strong>of</strong> crude Escherichia coli proteins. Because <strong>of</strong> the simplicity <strong>of</strong> the immobilization through " click<br />
chemistry" , the new ligand 2 is expected to not only <strong>of</strong>fer improved glycoprotein separation in other formats, but also act as<br />
a useful building block to develop new chemical sensors for analysis <strong>of</strong> other glycan compounds. © <strong>2010</strong> Elsevier B.V.<br />
No.1394<br />
Author (s) : Sumethkul, V., Tankee, P., Chalermsanyakorn, P., Jirasiritham, S.<br />
Title : Long-term outcome <strong>of</strong> very early cyclosporine minimization and de novo everolimus therapy in kidney transplant recipients:<br />
A pharmacokinetic guided approach<br />
Source : Transplantation Proceedings, Volume 42, Issue 10, December <strong>2010</strong>, Pages 4040-4042<br />
Document Type : Conference Paper<br />
Keywords : creatinine, cyclosporin A, everolimus, prednisolone; area under the curve, clinical article, conference paper, creatinine blood<br />
level, drug dose reduction, follow up, glomerulus filtration rate, graft recipient, graft survival, human, kidney graft<br />
rejection, kidney transplantation, maximum plasma concentration, pharmacokinetics, plasma concentration-time<br />
curve, priority journal, survival, time to maximum plasma concentration<br />
<strong>Abstract</strong> : Background Cyclosporine (CsA) nephrotoxicity is an important cause <strong>of</strong> chronic allograft dysfunction. Clinical information<br />
concerning the impact <strong>of</strong> very early CsA dose reduction in kidney transplant recipients is limited. We have examined the longterm<br />
outcomes <strong>of</strong> very early CsA dose reduction. This is synchronized with de novo everolimus and steroid therapy. Methods<br />
We enrolled 10 de novo kidney transplant recipients to receive CsA (target C0 250350 ng/mL) and prednisolone as initial<br />
therapy. CsA dosage was reduced by 50% at posttransplant day 7. Everolimus (target trough level, 38 ng/mL) was<br />
concomitantly started at the day <strong>of</strong> CsA reduction. Full pharmacokinetic studies <strong>of</strong> everolimus and CsA were studied at the<br />
period <strong>of</strong> 48 weeks after CsA reduction. CsA was then gradually reduced to maintain a trough level <strong>of</strong> 50100 ng/mL and/or<br />
Cmax <600 ng/mL. Results The mean follow-up was 51.2 ± 3.45 months. The nadir serum creatinine was 1.03 ± 0.33 mg/dL.<br />
The mean initial estimated glomerular filtration rate (eGFR) was 97.97 ± 23.36 mL/min. The mean initial trough everolimus<br />
was 5.2 ± 1.5 ng/mL. The eGFR at 1 year, 3 years, and last follow-up was 82 ± 25, 80 ± 21, and 80 ± 25 mL/min, respectively.<br />
Patient and graft survival was 100%. Conclusion Very early CsA dose reduction synchronized with de novo everolimus therapy<br />
was associated with good long-term patient and graft survival in kidney transplant recipients. This intervention can lead to<br />
75% CsA minimization and is associated with very good GFR by the modification <strong>of</strong> Diet in Renal Disease Formula at year 4. ©<br />
<strong>2010</strong> Elsevier Inc. All rights reserved.<br />
No.1395<br />
Author (s) : Sumetphong, C., Tangwongsan, S.<br />
Title : Recognizing broken characters in Thai Historical documents<br />
Source : ICACTE <strong>2010</strong> - <strong>2010</strong> 3rd <strong>International</strong> Conference on Advanced Computer Theory and Engineering, Proceedings, Volume 1,<br />
<strong>2010</strong>, Article number 5579053, Pages V199-V1103<br />
Document Type : Conference Paper<br />
Keywords : Broken characters; Error correction; Optical character recognition; Set-partitions; Thai historical documents<br />
<strong>Abstract</strong> : One <strong>of</strong> the biggest challenges in restoring historical documents is to achieve a high level <strong>of</strong> OCR accuracy. The main<br />
characteristic inherent to these valuable but degraded documents is the abundant presence <strong>of</strong> broken characters. This paper<br />
represents this problem as a mathematical model. We also propose a novel solution based on set-partitions to recognize<br />
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broken characters in Thai Historical documents. Experiments based on this solution have been performed and the results are<br />
very promising. © <strong>2010</strong> IEEE.<br />
No.1396<br />
Author (s) : Sumriddetchkajorn, K.; Chailurkit, L.; Thakkinstian, A.; Sritara, P.<br />
Title : HYPOVITAMINOSIS D IN THAILAND WAS ASSOCIATED WITH DECREASED RISK FOR HYPERTENSION<br />
Source : JOURNAL OF HYPERTENSION, Volume: 28, Supplement: A, Pages: E131-E132, Published: JUN <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1397<br />
Author (s) : Sumruayphol, S., Apiwathnasorn, C., Komalamisra, N., Ruangsittichai, J., Samung, Y., Chavalitshewinkoon-Petmitr, P.<br />
Title : Bionomic status <strong>of</strong> Anopheles epiroticus Linton & Harbach, a coastal malaria vector, in Rayong Province, Thailand<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 3, May <strong>2010</strong>, Pages 541-547<br />
Document Type : Article<br />
Keywords : Anopheles epiroticus; Bionomic status; Malaria vector; Thailand<br />
<strong>Abstract</strong> : A longitudinal entomological survey was conducted to provide in-depth information on An. epiroticus and determine whether<br />
ecological and entomological factors could influence malaria transmission in Rayong Province, Thailand. The mosquitoes were<br />
collected monthly from May 2007 to April 2008 by human landing catch technique from 6:00-12:00 PM for 2 consecutive<br />
nights, at 3 collection sites. A total <strong>of</strong> 3,048 mosquitoes within 5 species were captured: An. epiroticus, Culex quinquefasciatus<br />
Say, Cx. sitiens Wiedemann, Aedes aegypti (L.) and Ae. albopictus Skuse. PCR was used for molecular identification <strong>of</strong> An.<br />
sundaicus complex, by determination <strong>of</strong> COI, ITS2, and D3 genes. The target mosquitoes were An. epiroticus, which was the<br />
predominant species, accounting for 43.8% <strong>of</strong> specimens collected. The biting cycle pattern increased during 6:00-8:00 PM<br />
and reached a maximum <strong>of</strong> 6.6 bites/person/hour by 12:00 PM. The mosquitoes varied in population density throughout the<br />
year. The highest biting rate was 37.6 bites/person/ half night in September and the lowest (10.2 bites/person/half night) in<br />
January. Nested PCR and real-time PCR techniques were used to detect the malaria parasite in An. epiroticus adult females.<br />
Nine <strong>of</strong> 926 (0.97%) mosquitoes tested were malaria parasite positive: 6 P. falciparum and 3 P. vivax. The infective mosquitoes<br />
were found in the dry and early rainy seasons. The overall annual entomological inoculation rate (EIR) in the village was 76.6.<br />
The overall parity rate was 74%. A total <strong>of</strong> 38 cement tanks were used to characterize the nature <strong>of</strong> the breeding places <strong>of</strong><br />
An. epiroticus. An. epiroticus larvae coexisted with Aedes and Culex larvae; the maximum larval density was more than 140<br />
larvae per dip in May. Breeding places included fresh, brackish and salt water, typically with full sunlight and mats <strong>of</strong> green<br />
algae on the water surface. The salinity <strong>of</strong> the water ranged from 0.5 to 119.4 g/l, with a narrow pH range <strong>of</strong> 8.2-8.7. Dissolved<br />
oxygen was highest in November (6.27 mg/l) and lowest in March (3.46 mg/l). The water temperature varied between 24.6<br />
and 32.8oC.<br />
No.1398<br />
Author (s) : Sun, W., Mao, S., Wang, Y., Junyaprasert, V.B., Zhang, T., Na, L., Wang, J.<br />
Title : Bioadhesion and oral absorption <strong>of</strong> enoxaparin nanocomplexes<br />
Source : <strong>International</strong> Journal <strong>of</strong> Pharmaceutics, Volume 386, Issue 1-2, 15 February <strong>2010</strong>, Pages 275-281<br />
Document Type : Article<br />
Keywords : Bioadhesion; Chitosan; Enoxaparin; Oral absorption; PEGylation; Polyelectrolyte complex; Thiolated chitosan; Trimethyl<br />
chitosan<br />
<strong>Abstract</strong> : Polyelectrolyte complexes (PEC) formed between chitosan derivatives and enoxaparin were prepared by a self-assembly<br />
process and were characterized in terms <strong>of</strong> particle size and surface charge. The morphology was observed by atomic force<br />
microscopy (AFM). The colloidal stability and bioadhesion <strong>of</strong> the PEC were characterized by dynamic light scattering (DLS).<br />
The absorption <strong>of</strong> enoxaparin in rats was evaluated by activated partial thromboplastin time (APTT) assay. It was shown that<br />
the prepared PEC had a spherical shape with positive charge and a mean diameter in the range <strong>of</strong> 200-600 nm. An increase in<br />
temperature led to a decrease in particle size (ca. 10%) with an increased kcps value (ca. 10-20%) for the PEC studied,<br />
depending on the polymer structure. Thiolation and methylation <strong>of</strong> chitosan could significantly improve the corresponding<br />
PEC's bioadhesion and hence the oral absorption <strong>of</strong> enoxaparin. A good relationship between bioadhesion and in vivo<br />
absorption was established. However, PEC <strong>of</strong> PEGylated chitosan did not display a significantly enhanced permeation <strong>of</strong><br />
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enoxaparin compared with unmodified chitosan. In conclusion, the oral bioavailability <strong>of</strong> enoxaparin can be enhanced by<br />
improving the bioadhesive properties <strong>of</strong> PEC via the chemical modification <strong>of</strong> chitosan employed. © 2009 Elsevier B.V. All<br />
rights reserved.<br />
No.1399<br />
Author (s) : Sundarathiti, P., Pasutharnchat, K., Jommaroeng, P.<br />
Title : Thoracic epidural-general analgesia in scoliosis surgery<br />
Source : Journal <strong>of</strong> Clinical Anesthesia, Volume 22, Issue 6, September <strong>2010</strong>, Pages 410-414<br />
Document Type : Article<br />
Keywords : Epidural; General administration; Thoracic analgesia; Thoracolumbar scoliosis surgery<br />
<strong>Abstract</strong> : Study Objective: To evaluate the efficacy <strong>of</strong> thoracic epidural analgesia (TEA) in scoliosis surgery. Design: Descriptive clinical<br />
study. Setting: <strong>University</strong> hospital. Patients: 15 ASA physical status I, II, and III patients undergoing thoracolumbar scoliosis<br />
correction. Interventions: TEA was performed at three to 5 cm cephalad to the incision, and 5 to 10 mL <strong>of</strong> 0.125% - 0.2%<br />
levobupivacaine was given initially. Then, 5 to 10 mL <strong>of</strong> levobupivacaine was infused hourly throughout the operation.<br />
General anesthesia (GA) was induced with thiopental sodium (5 mg/kg) and fentanyl (one μg/kg) and was maintained with<br />
0.2% sev<strong>of</strong>lurane and 50% nitrous oxide in oxygen. Intraoperative epidural morphine (two to three mg) was administered, and<br />
0.1% levobupivacaine with morphine (0.04 to 0.08 mg/mL) was infused at two to 4 mL/hr for postoperative analgesia.<br />
Measurements: Adequacy <strong>of</strong> anesthesia, postanesthetic recovery and analgesia, adverse effects, and patient satisfaction were<br />
recorded. Main Results: 20% <strong>of</strong> patients underwent more than 10 levels <strong>of</strong> correction, and 53% had coexisting morbid<br />
diseases. All had adequate anesthesia. Immediately in the Postanesthesia Care Unit (PACU), 67% <strong>of</strong> patients reached an<br />
Aldrete score <strong>of</strong> 10, and 40% were fully awake and oriented. All patients were arousable to command and able to flex their<br />
hips and knees. None had intraoperative recall. 73% reported no pain in the PACU or 6 hours postoperatively. No serious<br />
adverse effects occured. 80% <strong>of</strong> patients rated their satisfaction as "good". Conclusions: Preincisional application <strong>of</strong> TEA with<br />
light GA may be used effectively in thoracolumbar scoliosis surgery. © <strong>2010</strong> Elsevier Inc. All rights reserved.<br />
No.1400<br />
Author (s) : Sungkanak, U., Sappat, A., Wisitsoraat, A., Promptmas, C., Tuantranont, A.<br />
Title : Ultrasensitive detection <strong>of</strong> Vibrio cholerae O1 using microcantilever-based biosensor with dynamic force microscopy<br />
Source : Biosensors and Bioelectronics, Volume 26, Issue 2, October <strong>2010</strong>, Pages 784-789<br />
Document Type : Article<br />
Keywords : Biosensor; Dynamic force microscopy; Microcantilever; Vibrio cholerae O1<br />
<strong>Abstract</strong> : This work presents the first demonstration <strong>of</strong> a cantilever based cholerae sensor. Dynamic force microscopy within atomic<br />
force microscope (AFM) is applied to measure the cantilever's resonance frequency shift due to mass <strong>of</strong> cell bound on<br />
microcantilever surface. The Vibrio cholerae O1, a food and waterborne pathogen that caused cholera disease in human, is a<br />
target bacterium cell <strong>of</strong> interest. Commercial gold-coated AFM microcantilevers are immobilized with monoclonal antibody<br />
(anti-V. cholerae O1) by self-assembled monolayer method. V. cholerae O1 detection experiment is then conducted in<br />
concentrations ranging from 1×103 to 1×107CFU/ml. The microcantilever-based sensor has a detection limit <strong>of</strong><br />
~1×103CFU/ml and a mass sensitivity, ∆m/∆F, <strong>of</strong> ~146.5pg/Hz, which is at least two orders <strong>of</strong> magnitude lower than other<br />
reported techniques and sufficient for V. cholerae detection in food products without pre-enrichment steps. In addition, V.<br />
cholerae O1 antigen-antibody binding on microcanilever is confirmed by scanning electron microscopy. The results<br />
demonstrate that the new biosensor is promising for high sensitivity, uncomplicated and rapid detection <strong>of</strong> V. cholerae O1. ©<br />
<strong>2010</strong> Elsevier B.V.<br />
No.1401<br />
Author (s) : Sungkanuparph, S.<br />
Title : Mortality rates in HIV-infected patients with second failure <strong>of</strong> antiretroviral therapy are still high: A lesson from NA-ACCORD<br />
Source : Expert Review <strong>of</strong> Anti-Infective Therapy, Volume 8, Issue 4, April <strong>2010</strong>, Pages 371-373<br />
Document Type : Note<br />
Keywords : Antiretroviral therapy; HIV; Mortality; Treatment failure; Virologic failure<br />
<strong>Abstract</strong> : In clinical practice, a significant proportion <strong>of</strong> HIV-infected patients still experience treatment failure during antiretroviral<br />
therapy (ART). There is limited information regarding the second treatment failure and its mortality rate. This article assessed<br />
the findings <strong>of</strong> a recently published paper describing analyzed data from the North American AIDS Cohort Collaboration on<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Research and Design. A total <strong>of</strong> 7159 out <strong>of</strong> 36,188 patients who received ART had a second virologic failure from ART.<br />
Although the risk <strong>of</strong> second failure decreased from 1996 to 2005 owing to the evolution <strong>of</strong> ART, the cumulative mortality at 5<br />
years after onset <strong>of</strong> second failure was 26%. Strategies to prevent treatment failure are urgently needed in order to minimize<br />
the mortality among HIV-infected patients receiving ART. © <strong>2010</strong> Expert Reviews Ltd.<br />
No.1402<br />
Author (s) : Sungkanuparph, S., Techasathit, W., Utaipiboon, C., Chasombat, S., Bhakeecheep, S., Leechawengwongs, M., Ruxrungtham, K.,<br />
Phanuphak, P.<br />
Title : Thai national guidelines for antiretroviral therapy in HIV-1 infected adults and adolescents <strong>2010</strong><br />
Source : Asian Biomedicine, Volume 4, Issue 4, August <strong>2010</strong>, Pages 515-528<br />
Document Type : Review<br />
Keywords : Adolescent; Adult; Antiretroviral; HIV; Thai guidelines<br />
<strong>Abstract</strong> : In Thailand, more than 150,000 patients are currently treated with antiretroviral drugs under the support <strong>of</strong> the National AIDS<br />
Program (NAP). The appointed Adults and Adolescents Committee consisted <strong>of</strong> 28 members who are experts in HIV research,<br />
patient care or health care policy. Relevant published literature, guidelines, and the most recent relevant clinical trials<br />
presented internationally were reviewed. Several peer review and clinical studies conducted in Thailand were included in the<br />
review process. Special considerations for patients with co-infection <strong>of</strong> tuberculosis or hepatitis B were incorporated.<br />
Appropriate cut-<strong>of</strong>f <strong>of</strong> CD4+ T-cell counts when to commence ART among Thai patients have been considered. It is now<br />
recommended to start ART at CD4+ T-cell count <350 cells/mm3. For treatment-naive patients, the preferred initial therapy<br />
is a nonnucleoside reverse transcriptase inhibitor (NNRTI)-based regimen containing lamivudine plus zidovudine or ten<strong>of</strong>ovir.<br />
Stavudine will be phased out in a two-year plan at the national program level. Viral load and CD4+ T-cell counts should be<br />
monitored at least once and twice a year. To achieve long-term treatment success, enhancing adherence together with the<br />
proper management <strong>of</strong> antiretroviral-related toxicity is critical. In summary, the major changes from the Thai 2008 guidelines<br />
include commencing ART earlier. ART is recommended regardless <strong>of</strong> CD4+ T cell count if patients have an indication to treat<br />
their HBV co-infection. Preferred first regimen uses AZT or TDF, not d4T as the NRTI-backbone. Furthermore, efavirenz is now<br />
considered a preferred NNRTI, along with nevirapine.<br />
No.1403<br />
Author (s) : Sungsuwan, S., Ruangsupapichart, N., Prabpai, S., Kongsaeree, P., Thongpanchang, T.<br />
Title : Tetrahydro-1,4-epoxynaphthalene-1-carboxylic acid: A chiral derivatizing agent for the determination <strong>of</strong> the absolute<br />
configuration <strong>of</strong> secondary alcohols<br />
Source : Tetrahedron Letters, Volume 51, Issue 38, <strong>2010</strong>, Pages 4965-4967<br />
Document Type : Article<br />
Keywords : alcohol, carboxylic acid derivative, proton, tetrahydro 1,4 epoxynaphthalene 1 carboxylic acid, unclassified drug;<br />
article, chemical structure, chirality, derivatization, proton nuclear magnetic resonance, reliability<br />
<strong>Abstract</strong> : A new chiral derivatizing agent, tetrahydro-1,4-epoxynaphthalene-1- carboxylic acid (THENA), with a represented synperiplanar<br />
disposition <strong>of</strong> O-Ca-CαO as a part <strong>of</strong> the bicyclic system to lock the aromatic residue conformation and the<br />
availability <strong>of</strong> an internal reference proton for 1H NMR spectral alignment, is introduced. In the determination <strong>of</strong> the absolute<br />
configuration <strong>of</strong> chiral secondary alcohols, THENA <strong>of</strong>fered good uniformity <strong>of</strong> ∆δ with high reliability, resulting in<br />
unambiguous assignment <strong>of</strong> the absolute configuration. © <strong>2010</strong> Elsevier Ltd. All rights reserved.<br />
No.1404<br />
Author (s) : Suntornsaratoon, P., Wongdee, K., Goswami, S., Krishnamra, N., Charoenphandhu, N.<br />
Title : Bone modeling in bromocriptine-treated pregnant and lactating rats: Possible osteoregulatory role <strong>of</strong> prolactin in lactation<br />
Source : American Journal <strong>of</strong> Physiology - Endocrinology and Metabolism, Volume 299, Issue 3, September <strong>2010</strong>, Pages E426-E436<br />
Document Type : Article<br />
Keywords : Bone histomorphometry; Hyperprolactinemia; Ion chromatography; Osteopenia; Uncoupling<br />
<strong>Abstract</strong> : The lactogenic hormone prolactin (PRL) directly regulates osteoblast functions in vitro and modulates bone remodeling in<br />
nulliparous rats, but its osteoregulatory roles in pregnant and lactating rats with physiological hyperprolactinemia remained<br />
unclear. Herein, bone changes were investigated in rats treated with bromocriptine (Bromo), an inhibitor <strong>of</strong> pituitary PRL<br />
release, or Bromo+PRL at different reproductive phases, from mid-pregnancy to late lactation. PRL receptors were strongly<br />
expressed in osteoblasts lining bone trabeculae, indicating bone as a target <strong>of</strong> PRL actions. By using dual energy X-ray<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
absorptiometry, we found a significant increase in bone mineral density in the femora and vertebrae <strong>of</strong> pregnant rats. Such<br />
pregnancy-induced bone gain was, however, PRL independent and may have resulted from the increased cortical thickness.<br />
Bone trabeculae were modestly changed during pregnancy as evaluated by bone histomorphometry. On the other hand,<br />
lactating rats, especially in late lactation, showed massive bone loss in bone trabeculae but not in cortical shells. Further<br />
study in Bromo- and Bromo+PRL-treated rats suggested that PRL contributed to decreases in trabecular bone volume and<br />
number and increases in trabecular separation and eroded surface, as well as a paradoxical increase in bone formation rate in<br />
late lactation. Uncoupling <strong>of</strong> trabecular bone formation and resorption was evident in lactating rats, with the latter being<br />
predominant. In conclusion, pregnancy mainly induced cortical bone gain, whereas lactation led to trabecular bone loss in<br />
both long bones and vertebrae. Although PRL was not responsible for the pregnancy-induced bone gain, it was an important<br />
regulator <strong>of</strong> bone modeling during lactation. Copyright © <strong>2010</strong> the American Physiological Society.<br />
No.1405<br />
Author (s) : Suntornsaratoon, P., Wongdee, K., Krishnamra, N., Charoenphandhu, N.<br />
Title : Femoral bone mineral density and bone mineral content in bromocriptine-treated pregnant and lactating rats<br />
Source : Journal <strong>of</strong> Physiological Sciences, Volume 60, Issue 1, January <strong>2010</strong>, Pages 1-8<br />
Document Type : Article<br />
Keywords : Bone density; Dual-energy X-ray absorptiometry (DXA); Femur; Lactation; Pregnancy<br />
<strong>Abstract</strong> : Since hyperprolactinemia was found to induce osteopenia in the metaphysis <strong>of</strong> long bone in non-mated female rats,<br />
pregnant and lactating rats with sustainedly high plasma prolactin (PRL) levels might also exhibit some changes in their long<br />
bones. We performed a longitudinal study in pregnant, lactating and post-weaning rats, using dual-energy X-ray<br />
absorptiometry to demonstrate site-specific changes (i.e., metaphysis vs. diaphysis) in femoral bone mineral density (BMD) and<br />
content (BMC). The results showed that femoral metaphyseal BMD and BMC were higher when compared to their agematched<br />
controls during pregnancy, before decreasing in late lactation and post-weaning. On the other hand, femoral<br />
diaphyseal BMC increased during pregnancy, early lactating and mid-lactating periods without change during late lactation and<br />
post-weaning. After 7 days <strong>of</strong> bromocriptine administration which inhibited endogenous PRL secretion, the lactation-induced<br />
increases in BMC during early and mid-lactating periods were abolished. Moreover, a decrease in metaphyseal BMD during<br />
late lactation was restored to the control levels by bromocriptine. However, bromocriptine did not antagonize the pregnancyinduced<br />
increases in BMD and BMC. It could be concluded that the effect <strong>of</strong> PRL on bone was variable during the<br />
reproductive periods. While having no effect on femoral BMD and BMC during pregnancy, PRL was responsible for bone gain<br />
in early and mid-lactating periods, but induced bone loss during late lactating period. © 2009 The Physiological Society <strong>of</strong><br />
Japan and Springer.<br />
No.1406<br />
Author (s) : Suntornsaratoon, P., Wongdee, K., Krishnamra, N., Charoenphandhu, N.<br />
Title : Possible chondroregulatory role <strong>of</strong> prolactin on the tibial growth plate <strong>of</strong> lactating rats<br />
Source : Histochemistry and Cell Biology, Volume 134, Issue 5, November <strong>2010</strong>, Pages 483-491<br />
Document Type : Article<br />
Keywords : Cartilage; Chondrocytes; Goldner's trichrome; Hyperprolactinemia; Immunohistochemistry; Prolactin receptor<br />
<strong>Abstract</strong> : Besides calcium accretion in the cortical envelope, a marked increase in the length <strong>of</strong> long bone was observed in pregnant<br />
and lactating rats, and thus the growth plate change was anticipated. Since several bone changes, such as massive trabecular<br />
bone resorption in late lactation, were found to be prolactin (PRL)-dependent, PRL may also be responsible for the maternal<br />
bone elongation. Herein, we investigated the growth plate change and possible chondroregulatory roles <strong>of</strong> PRL in the tibiae <strong>of</strong><br />
rats at mid-pregnancy until 15 days postweaning. We found that the tibial length <strong>of</strong> lactating rats was increased and was<br />
inversely correlated with the total growth plate height, as well as the heights <strong>of</strong> proliferating zone (PZ) and hypertrophic zone<br />
(HZ), but not the resting zone (RZ). Chondrocytes in all zones expressed PRL receptors as visualized by<br />
immunohistochemistry, suggesting that the growth plate cartilage was a target <strong>of</strong> PRL action. Further investigations in lactating<br />
rats treated with an inhibitor <strong>of</strong> pituitary PRL release, bromocriptine, with or without PRL supplement, revealed the PRLinduced<br />
decreases in total growth plate height and HZ height from early to late lactation. However, decreases in RZ and PZ<br />
heights were observed only in late and mid-lactation, respectively. Thus, this was the first report on the chondroregulatory<br />
action <strong>of</strong> PRL on the growth plate <strong>of</strong> long bone in lactating rats. The results provided better understanding <strong>of</strong> the maternal<br />
bone adaptation during lactation. © <strong>2010</strong> Springer-Verlag.<br />
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No.1407<br />
Author (s) : Suntornsuk, L.<br />
Title : Recent advances <strong>of</strong> capillary electrophoresis in pharmaceutical analysis<br />
Source : Analytical and Bioanalytical Chemistry, Volume 398, Issue 1, September <strong>2010</strong>, Pages 29-52<br />
Document Type : Review<br />
Keywords : Active pharmaceutical ingredient; Biological fluid; Capillary electrophoresis; Chiral separation; Impurity<br />
<strong>Abstract</strong> : This review covers recent advances <strong>of</strong> capillary electrophoresis (CE) in pharmaceutical analysis. The principle, instrumentation,<br />
and conventional modes <strong>of</strong> CE are briefly discussed. Advances in the different CE techniques (non-aqueous CE,<br />
microemulsion electrokinetic chromatography, capillary isotachophoresis, capillary electrochromatography, and<br />
immunoaffinity CE), detection techniques (mass spectrometry, light-emitting diode, fluorescence, chemiluminescence, and<br />
contactless conductivity), on-line sample pretreatment (flow injection) and chiral separation are described. Applications <strong>of</strong> CE<br />
to assay <strong>of</strong> active pharmaceutical ingredients (APIs), drug impurity testing, chiral drug separation, and determination <strong>of</strong> APIs in<br />
biological fluids published from 2008 to 2009 are tabulated. © <strong>2010</strong> Springer-Verlag.<br />
No.1408<br />
Author (s) : Suntornsuk, L., Ployngam, S.<br />
Title : Simultaneous determination <strong>of</strong> R-(-)-, S-(+)-bacl<strong>of</strong>en and impurity A by electrokinetic chromatography<br />
Source : Journal <strong>of</strong> Pharmaceutical and Biomedical Analysis, Volume 51, Issue 3, 5 February <strong>2010</strong>, Pages 541-548<br />
Document Type : Article<br />
Keywords : Bacl<strong>of</strong>en enantiomers; Cyclodextrin; Electrokinetic chromatography; Impurity A; Polyethylene oxide<br />
<strong>Abstract</strong> : A rapid method for the simultaneous analysis <strong>of</strong> R-(-)-, S-(+)-bacl<strong>of</strong>en and impurity A, (4RS)-4-(4-chlorophenyl) pyrrolidin-2one,<br />
by electrokinetic chromatography was established. The optimized condition was in 100 mM sodium borate buffer (pH<br />
9.9) containing 18 mM α-cyclodextrin (CD) and 1% (v/v) ACN using a fused-silica capillary dynamically coated with<br />
polyethylene oxide (PEO), with an effective length <strong>of</strong> 56 cm and an inner diameter <strong>of</strong> 50 μm, hydrodynamic injection at 50<br />
mbar for 6 s, temperature <strong>of</strong> 45 °C, applied voltage <strong>of</strong> 27 kV and UV detection at 220 nm. Baseline separation <strong>of</strong> all analytes<br />
was achieved within 9 min (Rs > 2.7) with the migration order <strong>of</strong> impurity A, S-(+)- and R-(-)-bacl<strong>of</strong>en. The method showed<br />
good linearity (r2 > 0.999 in a range <strong>of</strong> 5-50 μg/mL for impurity A and 50-500 μg/mL for bacl<strong>of</strong>en enantiomers), precision<br />
(%RSDs < 3.37%) and recoveries (100.3% for R-(-)-bacl<strong>of</strong>en, 101.6% for S-(+)-bacl<strong>of</strong>en and 96.1% for impurity A). Detection<br />
and quantitation limits were 10 and 30 μg/mL for both enantiomers, and 2 and 5 μg/mL for the impurity, respectively. The<br />
method was efficient for the determination <strong>of</strong> bacl<strong>of</strong>en enantiomers and impurity A in pharmaceutical raw material and<br />
formulations due to its reliability, speed and simplicity. © 2009 Elsevier B.V. All rights reserved.<br />
No.1409<br />
Author (s) : Supa, W., Naruedomkul, K., Cercone, N.<br />
Title : Computer support for mathematical word problem solving: Guided by Thai teachers' views<br />
Source : CSEDU <strong>2010</strong> - 2nd <strong>International</strong> Conference on Computer Supported Education, Proceedings, Volume 1, <strong>2010</strong>, Pages 245-250<br />
Document Type : Conference Paper<br />
Keywords : Mathematical word problem; Natural language understanding; Teachers' views; Word problem solving<br />
<strong>Abstract</strong> : This study investigates Thai teachers' views in teaching and learning mathematical word problem, and the technology<br />
implementation to enhance teaching and learning mathematical word problem. Data were collected by use <strong>of</strong> semistructured<br />
interviews with 24 mathematics teachers. The result <strong>of</strong> this study showed that the students' major difficulty in<br />
solving mathematical word problem lies in the understanding <strong>of</strong> the problem and the translation <strong>of</strong> the problem into<br />
equations. Therefore, the teachers' suggestions led to the design <strong>of</strong> a tool to help improve students' performance in solving<br />
mathematical word problem.<br />
No.1410<br />
Author (s) : Supakdamrongkul, P., Bhumiratana, A., Wiwat, C.<br />
Title : Characterization <strong>of</strong> an extracellular lipase from the biocontrol fungus, Nomuraea rileyi MJ, and its toxicity toward Spodoptera<br />
litura<br />
Source : Journal <strong>of</strong> Invertebrate Pathology, Volume 105, Issue 3, November <strong>2010</strong>, Pages 228-235<br />
Document Type : Article<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : Biocontrol fungus; Biological control; Conidial germination; Entomopathogenic fungus; Enzymatic<br />
characterization; Extracellular lipase; Hydrolytic enzyme; Nomuraea rileyi; Spodoptera litura; Triacylglycerides<br />
<strong>Abstract</strong> : An extracellular lipase from Nomuraea rileyi MJ was purified 23.9-fold with 1.69% yield by ammonium sulfate precipitation<br />
followed by Sephacryl S-100 HR column chromatography. By mass spectrometry and SDS-polyacrylamide gel electrophoresis,<br />
the molecular weight <strong>of</strong> the homogenous lipase was 81kDa. The N-terminal sequence was determined as<br />
LeuSerValGluGlnThrLysLeuSerLysLeuAlaTyrAsnAsp and it showed no homology to sequences <strong>of</strong> known lipases. The optimum<br />
pH and temperature for activity were 8.0 and 35°C, respectively. The enzyme was stable in the pH range 7.0-9.0 and at 15-<br />
35°C for 1h. Higher activity was observed in the presence <strong>of</strong> surfactants, Na+, NH4+ ions, NaN3 and<br />
ethylenediaminetetraacetic acid (EDTA), while Co2+ and Cu2+ ions, cysteine and dithiothreitol (DTT) strongly inhibited activity.<br />
The purified lipase hydrolyzed both synthetic and natural triglycerides with maximum activity for trilaurin and coconut oil,<br />
respectively. It also hydrolyzed esters <strong>of</strong> p-nitrophenol (pNP) with highest activity for p-nitrophenyl caprate (pNPCA). The<br />
purified lipase was found to promote N. rileyi spore germination in vitro in that germination reached 98% in conidial<br />
suspensions containing purified lipase at 2.75 U. Moreover, it enhanced toxicity <strong>of</strong> N. rileyi toward Spodoptera litura larvae<br />
with mortality via topical application reaching 63.3% at 4-10days post-treatment which calculated to be 2.7 times higher than<br />
the mortality obtained using conidial suspensions alone. © <strong>2010</strong> Elsevier Inc.<br />
No.1411<br />
Author (s) : Supakdamrongkul, P.; Bhumiratana, A.; Wiwat, C.<br />
Title : Optimization <strong>of</strong> extracellular lipase production from the biocontrol fungus Nomuraea rileyi<br />
Source : BIOCONTROL SCIENCE AND TECHNOLOGY, Volume: 20, Issue: 6, Pages: 595-604, DOI: 10.1080/09583151003661177, Published:<br />
<strong>2010</strong><br />
Document Type : Article<br />
Keywords : biocontrol fungus; extracellular lipase; hydrolytic enzyme; lipase activity; Nomuraea rileyi; production<br />
<strong>Abstract</strong> : Lipases are important cuticle-degrading enzymes that hydrolyze the ester bonds <strong>of</strong> waxes, fats and lipoproteins during the<br />
infection <strong>of</strong> insects by the fungus Nomuraea rileyi. Lipase production by the N. rileyi strain MJ was optimized by varying<br />
environmental and nutritional conditions in culture medium containing different vegetable oils at various concentrations with<br />
shaking at 150 rpm for 8 days at 25 degrees C. The maximum lipase production was obtained using castor oil (30.5 +/- 0.6 U<br />
mL(-1)), followed in order by coconut oil (20.8 +/- 0.4 U mL(-1)), olive oil (20.8 +/- 0.4 U mL(-1)) and cottonseed oil (20.6 +/-<br />
0.4 U mL(-1)). The highest lipase activity (37.7 +/- 0.4 U mL(-1)) was obtained when castor oil was used at a concentration <strong>of</strong><br />
4% (v/v) <strong>of</strong> basal medium. When the surfactant Tween 80 was added at the fourth day rather than at the beginning <strong>of</strong><br />
incubation, a maximum lipase activity <strong>of</strong> 44.9 +/- 3.5 U mL(-1) was obtained. The optimal temperature and pH for lipase<br />
production were 25 degrees C and pH 8.0, respectively. This is the first report on lipase production by the biocontrol fungus<br />
N. rileyi.<br />
No.1412<br />
Author (s) : Suparb, A.; Monrudee, P.<br />
Title : Osteoporosis knowledge, health beliefs and preventive behavior in older women living in a rural area<br />
Source : JOURNAL OF CLINICAL NURSING, Volume: 19, Supplement: 1, Pages: 26-26, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1413<br />
Author (s) : Supasai, T., Dangtip, S., Learngarunsri, P., Boonyopakorn, N., Wisitsoraat, A., Hodak, S.K.<br />
Title : Influence <strong>of</strong> temperature annealing on optical properties <strong>of</strong> SrTiO3/BaTiO3 multilayered films on indium tin oxide<br />
Source : Applied Surface Science, Volume 256, Issue 14, 1 May <strong>2010</strong>, Pages 4462-4467<br />
Document Type : Article<br />
Keywords : Annealing; Multilayers; Optical properties; Perovskites; Sol-gel<br />
<strong>Abstract</strong> : We have prepared SrTiO3/BaTiO3 thin films with multilayered structures deposited on indium tin oxide (ITO) coated glass by a<br />
sol-gel deposition and heating at 300-650° C. The optical properties were obtained by UV-vis spectroscopy. The films show a<br />
high transmittance (approximately 85%) in the visible region. The optical band gap <strong>of</strong> the films is tunable in the 3.64-4.19 eV<br />
range by varying the annealing temperature. An abrupt decrease towards the bulk band gap value is observed at annealing<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
temperatures above 600° C. The multilayered film annealed at 650° C exhibited the maximum refractive index <strong>of</strong> 2.09-1.91 in<br />
the 450-750 nm wavelength range. The XRD and AFM results indicate that the films annealed above 600° C are substantially<br />
more crystalline than the films prepared at lower temperatures which were used to change their optical band gap and<br />
complex refractive index to an extent that depended on the annealing temperature. © <strong>2010</strong> Elsevier B.V. All rights reserved.<br />
No.1414<br />
Author (s) : Suphamungmee, W., Chansela, P., Weerachatyanukul, W., Poomtong, T., Vanichviriyakit, R., Sobhon, P.<br />
Title : Ultrastructure, composition, and possible roles <strong>of</strong> the egg coats in haliotis asinine<br />
Source : Journal <strong>of</strong> Shellfish Research, <strong>2010</strong>, November 1, 29 (3), pp. 687-697.<br />
Document Type : Conference Paper<br />
Keywords : abalone; acrosome reaction; egg jelly coat; Haliotis asinina; motility; vitelline envelope<br />
<strong>Abstract</strong> : Spawned eggs <strong>of</strong> a tropical abalone Haliotis asinina have 2 protective barriers: the egg jelly coat and the vitelline envelope. At<br />
the electron microscopic level, the egg jelly is composed <strong>of</strong> a network <strong>of</strong> large fibers (4050 nm thick) cross-linked by smaller<br />
fibers (1520 nm thick), whereas the vitelline envelope is a thin, tough sheet containing pores that might be channels for<br />
sperm contact and entry. Electrophoretically, the egg jelly contains 2 major glycoproteins at 107 kDa and 178 kDa, whereas<br />
the vitelline envelope contains a broad spectrum <strong>of</strong> protein bands ranging from 15200 kDa, which also includes the<br />
corresponding egg jelly protein bands. Glycoproteins <strong>of</strong> egg jelly and vitelline envelope exhibit strong cross-reactivities, and<br />
they appear in late oocytes (Oc4, Oc5). Glucose is the major sugar composition <strong>of</strong> both egg jelly and vitelline envelope<br />
glycoproteins, whereas minor proportions <strong>of</strong> arabinose, fructose, galactose, and fucose are present in both the egg jelly and<br />
vitelline envelope. Our findings suggest that a sperm acrosome reaction could be induced by isolated vitelline envelope<br />
glycoproteins, whereas acceleration <strong>of</strong> sperm motility could be stimulated by egg jelly glycoproteins.<br />
No.1415<br />
Author (s) : Suphamungmee, W., Engsusophon, A., Vanichviriyakit, R., Sretarugsa, P., Chavadej, J., Poomtong, T., Linthong, V., Sobhon, P.<br />
Title : Proportion <strong>of</strong> sperm and eggs for maximal in vitro fertilization in haliotis asinina and the chronology <strong>of</strong> early development<br />
Source : Journal <strong>of</strong> Shellfish Research, Volume 29, Issue 3, November <strong>2010</strong>, Pages 757-763<br />
Document Type : Conference Paper<br />
Keywords : abalone; early development; egg; fertilization; Haliotis asinina; sperm<br />
<strong>Abstract</strong> : To obtain the highest yield during in vitro fertilization <strong>of</strong> tropical abalone Haliotis asinina, optimal proportion <strong>of</strong> the gametes,<br />
the timing <strong>of</strong> sperm-egg interaction, and subsequent development were investigated. The highest yield <strong>of</strong> fertilization (75%)<br />
with fewest abnormal eggs was obtained when incubating eggs and sperm at the ratio <strong>of</strong> 1:100 in seawater with a salinity <strong>of</strong><br />
27.5 ppt, a pH <strong>of</strong> 7.8, and a temperature range from 2729°C. After incubation, sperm swim through the eggjelly coat and<br />
become bound to the vitelline envelope within 30 sec, followed by an acrosomal reaction at 1 min. The fertilized egg<br />
extrudes the first and second polar bodies at 810 min, and then the zygote begins cleavage at 1520 min. This is followed by<br />
the second cleavage, and development through the stages <strong>of</strong> blastula, gastrula, trochophore, veliger, and early creeping<br />
larvae, which were completed within 3 days. Noticeably, occurrence <strong>of</strong> egg jelly condensation after penetration <strong>of</strong> the first<br />
sperm would not allow other sperm bind to the egg jelly and to penetrate through its vitelline envelope. This event is<br />
thought to be a weak blocking against polyspermy, because the classic cortical reaction initiated by cortical granule<br />
exocytosis could not be observed in this species.<br />
No.1416<br />
Author (s) : Suppapittayaporn, D., Emarat, N., Arayathanitkul, K.<br />
Title : The effectiveness <strong>of</strong> peer instruction and structured inquiry on conceptual understanding <strong>of</strong> force and motion: A case study<br />
from Thailand<br />
Source : Research in Science and Technological Education, Volume 28, Issue 1, April <strong>2010</strong>, Pages 63-79<br />
Document Type : Article<br />
Keywords : Conceptual change; Force and motion; Peer instruction; Structured inquiry<br />
<strong>Abstract</strong> : This study proposed to investigate the effectiveness <strong>of</strong> learning activities based on a conceptual change theoretical<br />
framework by embedding a peer instruction method with structured inquiry (PISI) on tenth grade students' understanding <strong>of</strong><br />
force and motion concepts. This teaching method was compared to the existing traditional instruction (TI). Alternative<br />
conceptions <strong>of</strong> force and motion were determined through related literature and a preliminary study, carried out prior to<br />
instruction, was used for designing the teaching plans. A standardized test, Force and Motion Conceptual Evaluation (FMCE),<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
was used to probe students' conceptual understanding <strong>of</strong> the subject. The data were obtained from 156 students in the<br />
treatment group taught with the PISI and 119 students in the control group taught with the TI. Hake's normalized gain<br />
indicated that the effectiveness <strong>of</strong> the TI was low (0.14) while the effectiveness <strong>of</strong> the PISI was medium (0.45). The Repeated<br />
Measures ANOVA comparing the pre- and post-test change in each and overall scales <strong>of</strong> the FMCE between the two groups<br />
was significant (p
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.1419<br />
Author (s) : Suputtamongkol, K., Wonglamsam, A., Eiampongpaiboon, T., Malla, S., Anusavice, K.J.<br />
Title : Surface roughness resulting from wear <strong>of</strong> lithia-disilicate-based posterior crowns<br />
Source : Wear, Volume 269, Issue 3-4, June <strong>2010</strong>, Pages 317-322<br />
Document Type : Article<br />
Keywords : Ceramic crown; Clinical; Roughness; Surface<br />
<strong>Abstract</strong> : The objective <strong>of</strong> this study was to determine the surface roughness that occurred as a result <strong>of</strong> wear <strong>of</strong> all-ceramic crowns<br />
after 2 years <strong>of</strong> intraoral service. The ceramic material used in this study was a lithia-disilicate-based all-ceramic core ceramic<br />
and a glass-phase veneer ceramic. Posterior bilayer crowns were made for 30 subjects, who were recalled annually. At each<br />
recall time, the quality <strong>of</strong> crowns and adjacent gingival tissues were examined for acceptability, and impressions were made<br />
for replica models. The occlusal contacts <strong>of</strong> each ceramic crown were identified and surface roughness (Ra) measurement<br />
was performed on the replica model using a contact pr<strong>of</strong>ilometer. Mean surface roughness values were analyzed using a<br />
repeated measure ANOVA. The wear patterns <strong>of</strong> contact surfaces were also characterized using a scanning electron<br />
microscope. From 30 ceramic crowns, 12 premolars and 13 molars were used for surface roughness measurements. For molar<br />
crowns, the surface roughness (Ra) at baseline was 1.76±0.43μm and the roughness significantly increased after 1 year and 2<br />
years (p<0.05). For premolar crowns, the increase in roughness values was significantly different only after 2 years from<br />
1.49±0.22 to 1.9±0.44μm (p<0.05). Roughening <strong>of</strong> ceramic surfaces was observed over the entire occlusal area, especially in<br />
the contact zones. Loss <strong>of</strong> superficial ceramic material as a result <strong>of</strong> micr<strong>of</strong>racture was the reason for surface roughening. In<br />
conclusion, the surface roughness <strong>of</strong> ceramic crowns was significantly increased after 2 years. The increase in surface<br />
roughness indicated the degradation <strong>of</strong> the ceramic crown surfaces over time that resulted from the wear process. © <strong>2010</strong><br />
Elsevier B.V.<br />
No.1420<br />
Author (s) : Suputtamongkol, Y., Pongtavornpinyo, W., Lubell, Y., Suttinont, C., Hoontrakul, S., Phimda, K., Losuwanaluk, K., Suwancharoen,<br />
D., Silpasakorn, S., Chierakul, W., Day, N.<br />
Title : Strategies for diagnosis and treatment <strong>of</strong> suspected leptospirosis: A cost-benefit analysis<br />
Source : PLoS Neglected Tropical Diseases, Volume 4, Issue 2, February <strong>2010</strong>, Article number e610<br />
Document Type : Article<br />
Keywords : doxycycline, antiinfective agent; article, controlled study, cost benefit analysis, diagnostic procedure, drug<br />
cost, fever, human, lateral flow test, latex test, leptospirosis, micro capsule agglutination test, outcome assessment, sensitivity<br />
and specificity, adolescent, adult, aged, case management, comparative study, economics, female, male, middle<br />
aged, time, treatment outcome; Adolescent, Adult, Aged, Aged, 80 and over, Anti-Bacterial Agents, Case<br />
Management, Doxycycline, Female, Humans, Leptospirosis, Male, Middle Aged, Time Factors, Treatment Outcome, Young<br />
Adult<br />
<strong>Abstract</strong> : Background: Symptoms and signs <strong>of</strong> leptospirosis are non-specific. Several diagnostic tests for leptospirosis are available and<br />
in some instances are being used prior to treatment <strong>of</strong> leptospirosis-suspected patients. There is therefore a need to evaluate<br />
the cost-effectiveness <strong>of</strong> the different treatment strategies in order to avoid misuse <strong>of</strong> scarce resources and ensure best<br />
possible health outcomes for patients. Methods: The study population was adult patients, presented with uncomplicated<br />
acute febrile illness, without an obvious focus <strong>of</strong> infection or malaria or typical dengue infection. We compared the cost and<br />
effectiveness <strong>of</strong> 5 management strategies: 1) no patients tested or given antibiotic treatment; 2) all patients given empirical<br />
doxycycline treatment; patients given doxycycline when a patient is tested positive for leptospirosis using: 3) lateral flow; 4)<br />
MCAT; 5) latex test. The framework used is a cost-benefit analysis, accounting for all direct medical costs in diagnosing and<br />
treating patients suspected <strong>of</strong> leptospirosis. Outcomes are measured in length <strong>of</strong> fever after treatment which is then<br />
converted to productivity losses to capture the full economic costs. Findings: Empirical doxycycline treatment was the most<br />
efficient strategy, being both the least costly alternative and the one that resulted in the shortest duration <strong>of</strong> fever. The<br />
limited sensitivity <strong>of</strong> all three diagnostic tests implied that their use to guide treatment was not cost-effective. The most<br />
influential parameter driving these results was the cost <strong>of</strong> treating patients with complications for patients who did not<br />
receive adequate treatment as a result <strong>of</strong> incorrect diagnosis or a strategy <strong>of</strong> no-antibiotic-treatment. Conclusions: Clinicians<br />
should continue treating suspected cases <strong>of</strong> leptospirosis on an empirical basis. This conclusion holds true as long as policy<br />
makers are not prioritizing the reduction <strong>of</strong> use <strong>of</strong> antibiotics, in which case the use <strong>of</strong> the latex test would be the most<br />
efficient strategy. © <strong>2010</strong> Suputtamongkol et al.<br />
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No.1421<br />
Author (s) : Surakul, P.; Vanichviriyakit, R.; Weerachatyanukul, W. A.; Jutapakdeegul, N.<br />
Title : Repeated maternal restraint stress down-regulated NMDA receptor subunits and scaffolding proteins in the hippocampus <strong>of</strong><br />
postnatal rat<br />
Source : INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE, Volume: 28, Issue: 8, Special Issue: SI, Pages: 686-686, DOI:<br />
10.1016/j.ijdevneu.<strong>2010</strong>.07.133, Published: DEC <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1422<br />
Author (s) : Surapolchai, P., Hongeng, S., Mahachoklertwattana, P., Pakakasama, S., Winaichatsak, A., Wisanuyothin, N., Pasomsub, E.,<br />
Mahasirimongkol, S., Sirachainan, N.<br />
Title : Impaired glucose tolerance and insulin resistance in survivors <strong>of</strong> childhood acute lymphoblastic leukemia: Prevalence and risk<br />
factors<br />
Source : Journal <strong>of</strong> Pediatric Hematology/Oncology, Volume 32, Issue 5, July <strong>2010</strong>, Pages 383-389<br />
Document Type : Article<br />
Keywords : Acute lymphoblastic leukemia; Children; Impaired glucose tolerance; Survivor<br />
<strong>Abstract</strong> : Aim/Purpose: Survivors <strong>of</strong> acute lymphoblastic leukemia (ALL) are at increased risks <strong>of</strong> impaired glucose metabolism, insulin<br />
resistance, and metabolic syndrome. The aim <strong>of</strong> our study was to determine the prevalence <strong>of</strong> alterations in glucose<br />
metabolism and the predisposing factors <strong>of</strong> these disturbances in survivors <strong>of</strong> childhood ALL. Patients and methods: in 131<br />
ALL survivors, an oral glucose tolerance test was conducted to determine β-cell function/insulin sensitivity. The particular risk<br />
factors were analyzed and 6 single nucleotide polymorphisms <strong>of</strong> diabetic predisposing genes: PAX4 and TCF7L2 were<br />
genotyped to evaluate the association between these factors and β-cell function/insulin sensitivity. Results: Ten out <strong>of</strong> 131<br />
survivors (7.6%) had impaired glucose tolerance (IGT) whereas 40 out <strong>of</strong> 131 (30.5%) had insulin resistance (IR) and showed<br />
characteristics <strong>of</strong> the metabolic syndrome (hyperinsulinemia, hypertriglyceridemia, and low HDL-C). in the logistic regression<br />
analysis, the most important factor predicting IGT and IR was older age <strong>of</strong> survivors (p = 0.014 and P < 0.001, respectively)<br />
whereas the PAX4 R192H mutation (rs2233580) was significantly associated with IGT after adjustment for age (P = 0.043)<br />
(adjusted OR 5.28, 95% CI 1.06-26.40). Conclusions: Existing evidence suggests that older age is an independent risk factor for<br />
developing IGT and IR in childhood ALL survivors, emphasizing the need for life-long metabolic screening. The PAX4 variant<br />
might impact individual susceptibility against IGT and diabetes. However, an identification <strong>of</strong> underlying risk(s) is the rational<br />
for future studies. Copyright © <strong>2010</strong> by Lippincott Williams & Wilkins.<br />
No.1423<br />
Author (s) : Suriyaprom, K., Namjuntra, P., Thawnasom, K., Pimainok, Y., Tungtrongchitr, R.<br />
Title : Association between cigarette smoking and metabolic syndrome in Thais<br />
Source : <strong>International</strong> Journal <strong>of</strong> Health Research, Volume 3, Issue 4, December <strong>2010</strong>, Pages 205-212<br />
Document Type : Article<br />
Keywords : Anthropometric variables; Biochemical parameters; Cigarette pack-years; Modified NCEP/ATP III criteria; Smoker<br />
<strong>Abstract</strong> : Purpose: To investigate the relationship between metabolic syndrome as defined by using the modified NCEP/ATP III criteria<br />
[modified the National Cholesterol Education Program (NCEP)/Adult Treatment Panel III (ATP III) criteria] and cigarette smoking<br />
in Thai subjects. Methods: This study was carried out among 254 smokers and 144 nonsmokers from suburban and urban<br />
residential areas in Bangkok, Thailand. All anthropometric variables, blood pressures, resting heart rate and biochemical<br />
parameters in each subject were measured. Results: The anthropometric variables, biochemical parameters, blood pressures<br />
and resting heart rate were not significantly different between smokers and nonsmokers, except for white blood cell count<br />
(WBC). Cigarette smoking was associated with increased risk for metabolic syndrome (OR =1.97; 95% CI=1.11-3.42) and the<br />
percentages <strong>of</strong> metabolic syndrome in smoker and nonsmoker Thais were 22.8% and 13.2%, respectively. Moreover, the<br />
number <strong>of</strong> cigarette smoking per day showed significant association with metabolic syndrome (p=0.047). Logistic regression<br />
analysis revealed that cigarette pack-years, resting heart rate, body mass index (BMI) and total cholesterol were significantly<br />
increased risk factors for metabolic syndrome. Conclusion: The current findings suggest that cigarette smoking is associated<br />
with the increased risk <strong>of</strong> metabolic syndrome by using the modified NCEP/ATP III criteria in Thais. © Poracom Academic<br />
Publishers. All rights reserved.<br />
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No.1424<br />
Author (s) : Suriyaprom, K., Phonrat, B., Namjuntra, P., Harnroongroj, T., Tungtrongchitr, R.<br />
Title : The-11377C > G adiponectin gene polymorphism alters the adiponectin concentration and the susceptibility to type 2<br />
diabetes in Thais<br />
Source : <strong>International</strong> Journal for Vitamin and Nutrition Research, Volume 80, Issue 3, <strong>2010</strong>, Pages 216-224<br />
Document Type : Article<br />
Keywords : Adiponectin; Gene polymorphism; Thais; Type 2 diabetes<br />
<strong>Abstract</strong> : The aims <strong>of</strong> this study were first to detect the levels <strong>of</strong> adiponectin, insulin, albumin, glucose, alanine aminotransferase (ALT),<br />
lipids, homeostasis model assessment for insulin resistance (HOMAIR), and anthropometric variables in type 2 diabetes<br />
mellitus (T2DM) as well as healthy control groups, and to determine whether two adiponectin gene polymorphisms, at the<br />
position-11377C > G as well as +45T > G, are associated with serum levels <strong>of</strong> adiponectin and other variables; then to search<br />
for the association between these two single nucleotide polymorphisms (SNPs) <strong>of</strong> the adiponectin gene and T2DM. We<br />
investigated 93 T2DM patients and 90 healthy volunteers. Compared with the healthy control group, the T2DM group had<br />
significantly lower adiponectin levels. Waist circumference, total cholesterol, ALT, glucose, insulin, and HOMA-IR scores were<br />
significantly higher in the T2DM group than in the control group. The polymorphism <strong>of</strong> the adiponectin gene at position-<br />
11377C > G among type 2 diabetes subjects showed that the adiponectin concentration was significantly lower in CG/GG<br />
genotypes (6.2 μg/mL) than the CC genotype (7.8 μg/mL), whereas SNP +45T > G was not associated with adiponectin levels.<br />
Adiponectin gene polymorphisms at position-11377C > G and +45T > G were significantly more frequent in type 2 diabetes<br />
patients than in the control group (p = 0.022; p = 0.045, respectively). However, multivariate logistic regression analysis<br />
showed that the strong impact on T2DM was found for-11377C > G gene polymorphism (p = 0.023) and waist circumference<br />
(p < 0.001). Therefore, the single nucleotide polymorphism <strong>of</strong>-11377C > G in adiponectin promoter region has impact on the<br />
lower adiponectin concentrations, and may influence susceptibility to T2DM in Thais. © Hogrefe & Huber Publishers.<br />
No.1425<br />
Author (s) : Sutapun, B., Somboonkaew, A., Amrit, R., Porntheeraphat, S., Houngkamhang, N., Srikhirin, T.<br />
Title : A multichannel SPR biosensor with a fixed detection system<br />
Source : Proceedings <strong>of</strong> SPIE - The <strong>International</strong> Society for Optical Engineering, Volume 7853, <strong>2010</strong>, Article number 78530Q<br />
Document Type : Conference Paper<br />
Keywords : Biosensor; Multichannel detection; Optical biosensor; SPR; Surface plasmon resonance<br />
<strong>Abstract</strong> : Surface plasmon resonance (SPR) sensors with spectral interrogation provide a high refractive index resolution, a large<br />
dynamic range and a fixed optical detection module. In this work, we propose a new spectral detection unit that uses only<br />
one spectrometer to measure the SPR spectrum from multiple sensing spots rapidly and serially without any mechanical<br />
movement. This spectral detection unit is designed based on a spatial light modulator (SLM) configured as a programmable<br />
optical aperture for the spectrometer. To demonstrate this concept, a five-channel SPR sensor was built based on the<br />
proposed multichannel detection unit and we evaluated the device performance using a refractive index test. The average<br />
device refractive index resolution was 1.4 × 10 -6 refractive index units (RIU). © <strong>2010</strong> SPIE.<br />
No.1426<br />
Author (s) : Suthammarak, W., Morgan, P.G., Sedensky, M.M.<br />
Title : Mutations in mitochondrial complex III uniquely affect complex I in Caenorhabditis elegans<br />
Source : Journal <strong>of</strong> Biological Chemistry, Volume 285, Issue 52, 24 December <strong>2010</strong>, Pages 40724-40731<br />
Document Type : Article<br />
Keywords : Caenorhabditis elegans, Complex i, Electron transport chain, Enzymatic activities, Mitochondrial complex, Mutant<br />
analysis, Respiratory chains, Stabilizing factors, Supercomplex; Mitochondria; Complexation; iron sulfur protein, iron sulfur<br />
protein 1, mitochondrial complex IV, mitochondrial DNA, protein ctb 1, reduced nicotinamide adenine dinucleotide<br />
dehydrogenase (ubiquinone), ubiquinol cytochrome c reductase, unclassified drug; article, Caenorhabditis elegans, enzyme<br />
activity, enzyme analysis, enzyme stability, gene mutation, mitochondrial respiration, nonhuman, phenotype, priority<br />
journal, protein interaction, proteomics; Animals, Caenorhabditis elegans, Caenorhabditis elegans Proteins, DNA,<br />
Helminth, DNA, Mitochondrial, Electron Transport Complex I, Electron Transport Complex III, Mitochondria, Mutation;<br />
Caenorhabditis elegans<br />
<strong>Abstract</strong> : Mitochondrial supercomplexes containing complexes I, III, and IV <strong>of</strong> the electron transport chain are now regarded as an<br />
established entity. Supercomplex I·III·IV has been theorized to improve respiratory chain function by allowing quinone<br />
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channeling between complexes I and III. Here, we show that the role <strong>of</strong> the supercomplexes extends beyond channeling.<br />
Mutant analysis in Caenorhabditis elegans reveals that complex III affects supercomplex I·III·IV formation by acting as an<br />
assembly or stabilizing factor. Also, a complex III mtDNA mutation, ctb-1, inhibits complex I function by weakening the<br />
interaction <strong>of</strong> complex IV in supercomplex I·III·IV. Other complex III mutations inhibit complex I function either by decreasing<br />
the amount <strong>of</strong> complex I (isp-1), or decreasing the amount <strong>of</strong> complex I in its most active form, the I·III·IV supercomplex (isp-<br />
1;ctb-1). ctb-1 suppresses a nuclear encoded complex III defect, isp-1, without improving complex III function. Allosteric<br />
interactions involve all three complexes within the supercomplex and are necessary for maximal enzymatic activities. © <strong>2010</strong><br />
by The American Society for Biochemistry and Molecular Biology, Inc.<br />
No.1427<br />
Author (s) : Sutherland, C.J., Tanomsing, N., Nolder, D., Oguike, M., Jennison, C., Pukrittayakamee, S., Dolecek, C., Hien, T.T., Do Rosário,<br />
V.E., Arez, A.P., Pinto, J., Michon, P., Escalante, A.A., Nosten, F., Burke, M., Lee, R., Blaze, M., Otto, T.D., Barnwell, J.W., Pain, A.,<br />
Williams, J., White, N.J., Day, N.P.J., Snounou, G., Lockhart, P.J., Chiodini, P.L., Imwong, M., Polley, S.D.<br />
Title : Two nonrecombining sympatric forms <strong>of</strong> the human malaria parasite plasmodium ovale occur globally<br />
Source : Journal <strong>of</strong> Infectious Diseases, Volume 201, Issue 10, 15 May <strong>2010</strong>, Pages 1544-1550<br />
Document Type : Article<br />
Keywords : Africa, article, Asia, cell lineage, controlled study, gene amplification, gene locus, gene segregation, gene sequence, genetic<br />
analysis, genetic trait, hominid, leaf dimorphism, malaria, multilocus sequence<br />
typing, Myanmar, Nigeria, nonhuman, nucleotide sequence, parasite isolation, phylogeny, Plasmodium ovale, priority<br />
journal, Sao Tome and Principe, sequence analysis, Sierra Leone, species identification, sympatry, Uganda, United<br />
Kingdom, animal, classification, genetic variability, genetics, genotype, health, human, parasitology; ribosome RNA;<br />
Animals, Genetic Variation, Genotype, Humans, Malaria, Phylogeny, Plasmodium ovale, RNA, Ribosomal, World Health<br />
<strong>Abstract</strong> : Background. Malaria in humans is caused by apicomplexan parasites belonging to 5 species <strong>of</strong> the genus Plasmodium.<br />
Infections with Plasmodium ovale are widely distributed but rarely investigated, and the resulting burden <strong>of</strong> disease is not<br />
known. Dimorphism in defined genes has led to P ovale parasites being divided into classic and variant types. We<br />
hypothesized that these dimorphs represent distinct parasite species. Methods. Multilocus sequence analysis <strong>of</strong> 6 genetic<br />
characters was carried out among 55 isolates from 12 African and 3 Asia-Pacific countries. Results. Each genetic character<br />
displayed complete dimorphism and segregated perfectly between the 2 types. Both types were identified in samples from<br />
Ghana, Nigeria, São Tomé, Sierra Leone, and Uganda and have been described previously in Myanmar. Splitting <strong>of</strong> the 2<br />
lineages is estimated to have occurred between 1.0 and 3.5 million years ago in hominid hosts. Conclusions. We propose that<br />
P ovale comprises 2 nonrecombining species that are sympatric in Africa and Asia. We speculate on possible scenarios that<br />
could have led to this speciation. Furthermore, the relatively high frequency <strong>of</strong> imported cases <strong>of</strong> symptomatic P ovale<br />
infection in the United Kingdom suggests that the morbidity caused by ovale malaria has been underestimated. © <strong>2010</strong> by<br />
the Infectious Diseases Society <strong>of</strong> America. All rights reserved.<br />
No.1428<br />
Author (s) : Suthikeeree, W., Lertdomrongdej, L., Charoensak, A.<br />
Title : Diagnostic performance <strong>of</strong> CT findings in differentiation <strong>of</strong> perforated from nonperforated appendicitis<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 12, December <strong>2010</strong>, Pages 1422-1429<br />
Document Type : Article<br />
Keywords : CT findings; Perforated appendicitis<br />
<strong>Abstract</strong> : Objective: To assess performance <strong>of</strong> CT finding in differentiation <strong>of</strong> perforated from non-perforated appendicitis in Siriraj<br />
hospital, Bangkok, Thailand and evaluate accuracy <strong>of</strong> each finding by using multidetector row computer tomography (MDCT).<br />
Material and Method: Between June 2006 and May 2009, all adult patients (age more than 15 years) who had a diagnosis <strong>of</strong><br />
acute appendicitis in Siriraj Hospital that was confirmed by pathology or surgical records and received a CT scan whole<br />
abdomen or lower abdomen with contrast administration before surgical or medical treatment were included in this study.<br />
The 48 consecutive CTs <strong>of</strong> whole or lower abdomen examinations were retrospectively reviewed by two radiologists.<br />
Sensitivity, specificity, and accuracy <strong>of</strong> CT compared with pathologic or surgical findings. Results: In 48 patients, 21 patients <strong>of</strong><br />
non-perforated appendicitis and 27 patients <strong>of</strong> perforated appendicitis were enrolled. The CT findings <strong>of</strong> abscess, extraluminal<br />
appendicolith, and extraluminal air had the highest specificities for perforated appendicitis, 95.24%, 100%, 95.24%,<br />
respectively. The best accurate finding was defect in enhancing appendiceal wall. The CT findings <strong>of</strong> enlarged mesenteric<br />
lymph nodes at right lower quadrant region and defect in enhancing appendiceal wall had the highest sensitivities, 88.88%<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
and 88.46%, respectively. Group 3 <strong>of</strong> combined specific findings had the best sensitivity (92.59%) and accuracy (83.3%).<br />
Conclusion: Multi-detector CT can help to differentiate perforated from non-perforated appendicitis.<br />
No.1429<br />
Author (s) : Suthisisang, C.C., Poolsup, N., Suksomboon, N., Lertpipopmetha, V., Tepwitukgid, B.<br />
Title : Meta-analysis <strong>of</strong> the efficacy and safety <strong>of</strong> naproxen sodium in the acute treatment <strong>of</strong> migraine<br />
Source : Headache, Volume 50, Issue 5, May <strong>2010</strong>, Pages 808-818<br />
Document Type : Article<br />
Keywords : Acute treatment; Meta-analysis; Migraine; Naproxen sodium; Systematic review<br />
<strong>Abstract</strong> : Objective. - To assess the efficacy and safety <strong>of</strong> naproxen sodium in the treatment <strong>of</strong> acute migraine attacks. Background. -<br />
Non-steroidal anti-inflammatory drugs including naproxen sodium have been used in treating migraine attack. A number <strong>of</strong><br />
clinical trials <strong>of</strong> naproxen sodium in migraine have been reported. However, it remains to be established whether naproxen<br />
sodium unequivocally <strong>of</strong>fers clinical benefits taken into account the desired outcomes in acute migraine therapy as<br />
recommended by the <strong>International</strong> Headache Society. Methods. - Clinical trials were identified through electronic searches<br />
(MEDLINE, EMBASE, EBM review, and the Cochrane Library) up to June 2009 and historical searches <strong>of</strong> relevant articles. Studies<br />
were included in the meta-analysis if they were (1) double-blind, randomized, placebo-controlled trials that evaluated<br />
naproxen sodium tablet in moderate or severe migraine attacks in adult patients, and (2) reporting the efficacy in terms <strong>of</strong><br />
headache relief, pain-free, relief <strong>of</strong> migraine-associated symptoms, sustained headache relief, sustained pain-free, or headache<br />
recurrence. Data extraction and study quality assessment were performed independently by 2 investigators. Disagreements<br />
were resolved by a third investigator. Treatment effects and adverse effects were expressed as risk ratio. A random effects<br />
model was used when significant heterogeneity existed, otherwise the fixed effects model was performed. Results. - We<br />
identified 16 published randomized controlled trials <strong>of</strong> naproxen in the treatment <strong>of</strong> migraine. Four trials met the inclusion<br />
criteria and were included in the meta-analysis. Naproxen sodium was more effective than placebo in reducing pain intensity<br />
and providing pain-free within 2 hours in adults with moderate or severe migraine attacks. The pooled risk ratios were 1.58<br />
(95% confidence interval 'CI'1.41-1.77, P
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
adolescents. The risk factors for fracture in thalassaemia patients included male gender, beta thalassaemia, splenectomy,<br />
transfusion and a low body mass index. Conclusion: A high prevalence <strong>of</strong> fracture is observed among Thais with thalassaemia.<br />
The aetiology was found to be multifactorial.<br />
No.1431<br />
Author (s) : Suttajit, S., Punpuing, S., Jirapramukpitak, T., Tangchonlatip, K., Darawuttimaprakorn, N., Stewart, R., Dewey, M.E., Prince, M.,<br />
Abas, M.A.<br />
Title : Impairment, disability, social support and depression among older parents in rural Thailand<br />
Source : Psychological Medicine, Volume 40, Issue 10, October <strong>2010</strong>, Pages 1711-1721<br />
Document Type : Article<br />
Keywords : Ageing; Asia; depression; developing countries; disability; social support<br />
<strong>Abstract</strong> : Background It is not known whether social support modifies the association between depression and impairment or disability<br />
in older people from developing countries in Asia. Method We used a Thai version <strong>of</strong> the EURO-D scale to measure<br />
depression in 1104 Thai rural community-dwelling parents aged 60 years. These were all those providing data on depression<br />
who were recruited as part <strong>of</strong> a study <strong>of</strong> older adults with at least one living child (biological, stepchild or adopted child).<br />
Logistic regression modelling was used to determine: (a) whether impairment, disability and social support deficits were<br />
associated with depression; (b) whether social support modified this association. Results There were strong graded<br />
relationships between impairment, disability, social support deficits and EURO-D caseness. Level <strong>of</strong> impairment, but not<br />
disability, interacted with poor social support in that depression was especially likely in those who had more physical<br />
impairments as well as one or more social support deficits (p value for interaction=0.018), even after full adjustment.<br />
Conclusions Social support is important in reducing the association between physical impairment and depression in Thai older<br />
adults, especially for those with a large number <strong>of</strong> impairments. Enhancing social support as well as improving healthcare and<br />
disability facilities should be emphasized in interventions to prevent depression in older adults. © Cambridge <strong>University</strong> Press<br />
<strong>2010</strong>.<br />
No.1432<br />
Author (s) : Sutthirak, P., Assavanig, A., Dharmsthiti, S., Lertsiri, S.<br />
Title : Changes In The Stability And Kinetic Parameters Up On Glycation Of Thermostable A-Amylase From Bacillus Subtilis<br />
Source : Journal <strong>of</strong> Food Biochemistry, Volume 34, Issue 6, December <strong>2010</strong>, Pages 1157-1171<br />
Document Type : Article<br />
Keywords : Acid hydrolysis, Bacillus Subtilis, Glycation, Maltodextrins, pI values, Protein bands; Bacilli, Bacteriology, Hydrolases, Kinetic<br />
parameters, Methanol, Reaction rates, Starch; Amylases; Bacillus subtilis<br />
<strong>Abstract</strong> : Glycation <strong>of</strong> the thermostable α-amylase, KLE, from Bacillus subtilis occurred during incubation with maltodextrin at 95C. This<br />
was revealed by the release <strong>of</strong> 5-hydroxymethyl-2-furfuraldehyde from the acid hydrolysis <strong>of</strong> glycated KLE (gKLE), the<br />
differences in the protein band patterns on SDS and Native-PAGE, and the shifting <strong>of</strong> the pI value from the range <strong>of</strong> 5.6-6.5 to<br />
that <strong>of</strong> 5.2-6.5. After glycation, the activity <strong>of</strong> gKLE was still retained. Furthermore, gKLE was more resistant to heat and pH<br />
compared with the nonglycated enzyme. The Km, reaction rate and efficiency to convert gelatinized cornstarch into<br />
maltodextrin <strong>of</strong> KLE were remained unchanged after glycation. This was different from the result obtained for BAN, another<br />
thermostable α-amylase produced by B. amyloliquefaciens. Glycation in BAN decreased the activity in converting gelatinized<br />
cornstarch into maltodextrin. Moreover, the stability and kinetic parameters <strong>of</strong> BAN were found to be negatively affected by<br />
glycation. © <strong>2010</strong>, Wiley Periodicals, Inc.<br />
No.1433<br />
Author (s) : Suwanjang, W., Phansuwan-Pujito, P., Govitrapong, P., Chetsawang, B.<br />
Title : The protective effect <strong>of</strong> melatonin on methamphetamine-induced calpain-dependent death pathway in human<br />
neuroblastoma SH-SY5Y cultured cells<br />
Source : Journal <strong>of</strong> Pineal Research, Volume 48, Issue 2, March <strong>2010</strong>, Pages 94-101<br />
Document Type : Article<br />
Keywords : Calpain; Calpastatin; Cell death; Melatonin; Methamphetamine; Neuroprotection; SH-SY5Y cells<br />
<strong>Abstract</strong> : Methamphetamine (METH) is a potent psychostimulant drug that may cause neuronal cell degeneration. The underlying<br />
mechanisms <strong>of</strong> METH-induced neuronal toxicity remains poorly understood. In this study, we investigated an important role<br />
<strong>of</strong> calpain-dependent cascades in methamphetamine-induced toxicity in human dopaminergic neuroblastoma SH-SY5Y<br />
521
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
cultured cell lines. In addition, the protective effect <strong>of</strong> melatonin against METH-induced calpain-dependent death pathway<br />
was also investigated. The results <strong>of</strong> this study show that METH significantly decreased cell viability and tyrosine hydroxylase<br />
phosphorylation in SH-SY5Y cultured cells. Melatonin reversed the toxic effect <strong>of</strong> METH by inducing cell viability. In addition,<br />
melatonin was able to restore the reduction in mitochondrial function and phosphorylation <strong>of</strong> tyrosine hydroxylase in SH-<br />
SY5Y treated cells. An induction <strong>of</strong> calpain expression and activity but a reduction <strong>of</strong> calpain inhibitor (calpastatin) protein<br />
levels were observed in SH-SY5Y cells treated with METH but these effects were diminished by melatonin. These results<br />
implicated calpain-dependent death pathways in the processes <strong>of</strong> METH-induced toxicity and also indicated that melatonin<br />
has the capacity to reverse this toxic effect in SH-SY5Y cultured cells. © 2009 John Wiley & Sons A/S.<br />
No.1434<br />
Author (s) : Suwanjang, W.; Phansuwan-Pujito, P.; Govitrapong, P.; Chetsawang, B.<br />
Title : CALPASTATIN RESCUES ACTIVATION OF CALPAIN PATHWAY IN METHAMPHETAMINE-INDUCED CELL DEATH IN SH-SY5Y<br />
CULTURED CELLS<br />
Source : JOURNAL OF NEUROCHEMISTRY, Volume: 115, Supplement: 1, Pages: 41-41, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1435<br />
Author (s) : Suwannalert, P., Rattanachitthawat, S., Chaiyasut, C., Riengrojpitak, S.<br />
Title : High levels <strong>of</strong> 25-hydroxyvitamin D3 [25(OH)D3] and α-tocopherol prevent oxidative stress in rats that consume Thai brown<br />
rice<br />
Source : Journal <strong>of</strong> Medicinal Plant Research, Volume 4, Issue 2, 18 January <strong>2010</strong>, Pages 120-124<br />
Document Type : Article<br />
<strong>Abstract</strong> : Oxidative stress has been proposed to play an important role in the progression <strong>of</strong> chronic diseases. The red color strain <strong>of</strong><br />
Thai brown rice, a high source <strong>of</strong> phenolic compounds, may play a crucial role in oxidative stress prevention. In the present<br />
study, rats were fed with 0% (Control), 10 and 70% Thai brown rice in the mixed food. The serum malondialdehyde (MDA),<br />
ferric reducing antioxidant power (FRAP), storage vitamin D [25(OH)D3] and α-tocopherol were investigated. The mean value<br />
<strong>of</strong> MDA in high and low dose groups was significantly lower than that <strong>of</strong> the controls in both male and female. FRAP in the<br />
high dose males was significantly higher than that in the control. Mean value <strong>of</strong> MDA and α-tocopherol was inversely related<br />
(r = -0.538, p =0.001). Interestingly, serum 25(OH)D3 <strong>of</strong> the high dose group was more significant different than that <strong>of</strong> the<br />
controls in both males (p = 0.001) and females (p = 0.005). Moreover, MDA level was strongly inversely related to that <strong>of</strong><br />
25(OH)D3 (r = -0.656, p < 0.001). The results indicated that the rats consuming Thai brown rice possessed low level <strong>of</strong><br />
oxidative stress marker, MDA, through both radical and non radical defenses. © <strong>2010</strong> Academic Journals.<br />
Keywords : α-tocopherol; Non radical; Oxidative stress; Radical; Vitamin D<br />
No.1436<br />
Author (s) : Suwannuraks, M.; Sri-Udomporn, N.; Im-Erbsin, T.<br />
Title : The strategy for creating the competence dentists for management <strong>of</strong> hemophilia patients in Thailand<br />
Source : HAEMOPHILIA, Volume: 16, Supplement: 4, Pages: 52-52, Published: JUL <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1437<br />
Author (s) : Suwanprathes, P., Won, C., Komoltri, C., Nana, A., Kotchabhakdi, N., Guilleminault, C.<br />
Title : Epidemiology <strong>of</strong> sleep-related complaints associated with sleep-disordered breathing in Bangkok, Thailand<br />
Source : Sleep Medicine, Volume 11, Issue 10, December <strong>2010</strong>, Pages 1025-1030<br />
Document Type : Article<br />
Keywords : Epidemiology; Excessive daytime sleepiness; Habitual snoring; Prevalence; Sleep-disordered breathing; Thailand<br />
<strong>Abstract</strong> : Background: This study assesses the prevalence <strong>of</strong> and risk factors for sleep-related complaints in Bangkok, Thailand.<br />
Methods: A representative sample <strong>of</strong> the Bangkok population was selected based on results <strong>of</strong> the 2000 Census. A total <strong>of</strong><br />
522
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
4680 participants underwent face-to-face interview with a 49-question sleep inventory. Results: Four percent <strong>of</strong> the total<br />
sampled (5.3% <strong>of</strong> men and 3.5% <strong>of</strong> women) complained <strong>of</strong> habitual snoring (>3 nights/week) and excessive daytime<br />
sleepiness (>3days/week) for at least 3months. These subjects were significantly (p<0.0001) older (41.4 vs. 36.7years), had<br />
greater BMI (26.0 vs. 22.8kg/m2), neck size (34.7 vs. 32.5cms), and waist circumference (88.0 vs. 78.7cms). They reported<br />
significantly shorter nocturnal sleep time, greater frequency <strong>of</strong> sleep disturbances and awakenings, unrefreshing sleep, choking<br />
during sleep, night sweats, nocturia, and bruxism. There was also a greater prevalence <strong>of</strong> cardiovascular and endocrine<br />
diseases. Multivariate analysis showed that male gender; BMI; waist size; and reports <strong>of</strong> witnessed apneas, unrefreshing sleep<br />
and night sweats were significant predictors <strong>of</strong> snoring and daytime sleepiness. Conclusion: This is the first epidemiologic<br />
study investigating sleep-related complaints and associated health morbidities in the Thai population. © <strong>2010</strong> Elsevier B.V.<br />
No.1438<br />
Author (s) : Suwansuksree, N., Thamlikitkul, V., Yamwong, P.<br />
Title : Drug use evaluation <strong>of</strong> statins at Siriraj Hospital, 2008.<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand = Chotmaihet thangphaet, Volume 93 Suppl 1, January <strong>2010</strong>, Pages S179-186<br />
Document Type : Article<br />
Keywords : generic drug, hydroxymethylglutaryl coenzyme A reductase inhibitor, simvastatin; adult, aged, article, clinical practice, drug<br />
use, economics, female, follow up, human, hypercholesterolemia, male, medical record, methodology, middle<br />
aged, outpatient, retrospective study, risk factor, statistics, Thailand, treatment outcome; Adult, Aged, Aged, 80 and over, Drug<br />
Utilization Review, Drugs, Generic, Female, Follow-Up Studies, Humans, Hydroxymethylglutaryl-CoA Reductase<br />
Inhibitors, Hypercholesterolemia, Male, Medical Records, Middle Aged, Outpatients, Physician's Practice Patterns, Retrospective<br />
Studies, Risk Factors, Simvastatin, Thailand, Treatment Outcome<br />
<strong>Abstract</strong> : Statins are commonly used for lipid reduction. There is no significant difference in the efficiency <strong>of</strong> each type <strong>of</strong> statins. The<br />
study <strong>of</strong> statins' efficacy shows that only generic simvastatin is cost-effective in coronary heart disease prevention. OBJECTIVE:<br />
To determine the use and appropriateness <strong>of</strong> usage <strong>of</strong> statins in out-patients attending Siriraj Hospital in 2008. MATERIAL AND<br />
METHOD: Medical records <strong>of</strong> all patients in Siriraj Hospital who received statins from January 1st to December 31st, 2008 were<br />
reviewed. The appropriateness <strong>of</strong> statins used was analyzed in 247 medical records based on number <strong>of</strong> risks and 10-year<br />
risk. RESULTS: There were 105,950 patients who received statins with total value <strong>of</strong> 308 million baht in 2008. The major<br />
usages <strong>of</strong> statins were simvastatin (65%), atorvastatin (12%) and rosuvastatin (6%). However, the costs <strong>of</strong> statins were 9%,<br />
42%, and 20% for simvastatin, atorvastatin and rosuvastatin, respectively. Analysis <strong>of</strong> 247 medical records <strong>of</strong> the patients who<br />
received statins showed that statins were appropriately used in 19.4% <strong>of</strong> cases. Inappropriate use <strong>of</strong> statins was due to not<br />
starting drugs treatment with simvastatin, or shifting from simvastatin to other statins inappropriately. CONCLUSION:<br />
Inappropriate use <strong>of</strong> statins at Siriraj Hospital is very common. Interventions for promoting more appropriate use <strong>of</strong> statins<br />
should be implemented.<br />
No.1439<br />
Author (s) : Suwanthanma, W.; Euanorasetr, C.<br />
Title : ANASTOMOSIS LEAKAGE IN COLORECTAL CANCER SURGERY: A PERSONAL SERIES AND LESSON TO LEARN FOR EARLY DIAGNOSIS<br />
USING THE LEAKAGE SCORE<br />
Source : ANNALS OF ONCOLOGY, Volume: 21, Supplement: 6, Pages: 34-34, Published: <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1440<br />
Author (s) : Suwatanapongched, P., Surarit, R., Srisatjaluk, R., Offenbacher, S.<br />
Title : Translocation <strong>of</strong> Porphyromonas gingivalis infected monocytes and sssociated cellular responses<br />
Source : Asian Pacific Journal <strong>of</strong> Allergy and Immunology, Volume 28, Issue 2-3, June <strong>2010</strong>, Pages 192-199<br />
Document Type : Article<br />
Keywords : ELISA; IL-1β; MCP-1; Monocyte; Monocyte migration; Porphyromonas gingivalis; TEM; TNF-α<br />
<strong>Abstract</strong> : Porphyromonas gingivalis (P.gingivalis), an important periodontal pathogen in adult chronic periodontitis, has been reported to<br />
colocalize in human atheromatous lesions. We have studied the phagocytosis and survival <strong>of</strong> P.gingivalis in human<br />
monocytes, together with the cellular responses <strong>of</strong> infected human monocytes. Human monocytes were cocultured with<br />
523
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
P.gingivalis and the external bacteria were killed with metronidazole and gentamycin. Localization <strong>of</strong> P.gingivalis in cells was<br />
studied by transmission electron microscopy (TEM). The survival <strong>of</strong> P. gingivalis was determined by lysing the monocytes and<br />
plating on blood agar under anaerobic conditions. Interleukin-1 beta (IL- 1β) and tumor necrosis factor-alpha (TNF-α) were<br />
determined using specific enzyme-linked immunosorbent assays (ELISAs) kits. The transwell chamber system was used to<br />
investigate the chemotactic response <strong>of</strong> the infected cells. TEM showed that P.gingivalis organisms were localized within the<br />
autophagosome-like structure <strong>of</strong> monocytes. No significant difference on the survival <strong>of</strong> P.gingivalis at 0, 4 and 8 h after<br />
infection was found. IL-1β and TNF-α were present in the cell culture media in response to bacterial challenge. The infected<br />
monocytes showed a normal chemotactic response to monocyte chemotactic protein-1 (MCP-1). The number <strong>of</strong> monocyte<br />
cells migrating through membrane in the presence and absence <strong>of</strong> P.gingivalis were 18.64±2.33×104 cells and 19.11±1.76×104<br />
cells respectively. The number <strong>of</strong> viable P.gingivalis per monocyte following translocation in response to the chemotactic<br />
gradient was 5.83±1.45×10-3 CFU/cell. The results indicate that P.gingivalis can stimulate cytokine production and survive in<br />
monocytes without affecting cell migration.<br />
No.1441<br />
Author (s) : Suzuki, T., Osaka, N., Endo, H., Shibayama, M., Ikeda, Y., Asai, H., Higashitani, N., Kokubo, Y., Kohjiya, S.<br />
Title : Nonuniformity in cross-linked natural rubber as revealed by contrast-variation small-angle neutron scattering<br />
Source : Macromolecules, Volume 43, Issue 3, 9 February <strong>2010</strong>, Pages 1556-1563<br />
Document Type : Article<br />
Keywords : Cluster-like structure, Cross-linked natural rubber, Crosslinker, Deuterated solvents, Di-cumyl peroxides, Elastic<br />
properties, Inhomogeneities, Microscopic structures, Nonuniformity, Partial scattering, Protein aggregates, Small-angle neutron<br />
scattering, Structural aspects; Deuterium, Neutron diffraction, Oxidation, Proteins, Rubber; Neutron scattering<br />
<strong>Abstract</strong> : The microscopic structures <strong>of</strong> cross-linked natural rubber (NR) were investigated by means <strong>of</strong> contrast-variation small-angle<br />
neutron scattering (CV-SANS) coupled with "visualization-by-swelling method" as a function <strong>of</strong> dicumyl peroxide (DCP; crosslinker)<br />
content, where the various types <strong>of</strong> inhomogeneities in the rubber were visualized by swelling with deuterated<br />
solvent. Detailed analyses <strong>of</strong> the partial scattering functions <strong>of</strong> each component confirm the existence <strong>of</strong> network<br />
inhomogeneities due to cluster-like structures <strong>of</strong> polyisoprene chains as well as larger inhomogeneities <strong>of</strong> protein aggregates.<br />
The observed partial scattering functions <strong>of</strong> polyisoprene with different DCP contents clearly exhibited that (1) the network<br />
inhomogeneities were strongly suppressed by DCP addition and (2) the structure <strong>of</strong> protein aggregates was not significantly<br />
influenced by the introduction <strong>of</strong> the peroxide cross-linking. These nanoscopic structural aspects with respect to the content<br />
<strong>of</strong> cross-linker provide better understanding <strong>of</strong> the elastic properties <strong>of</strong> NR. © <strong>2010</strong> American Chemical Society.<br />
No.1442<br />
Author (s) : Svasti, S., Masaki, S., Penglong, T., Abe, Y., Winichagoon, P., Fucharoen, S., Umemura, T.<br />
Title : Expression <strong>of</strong> microRNA-451 in normal and thalassemic erythropoiesis<br />
Source : Annals <strong>of</strong> Hematology, Volume 89, Issue 10, October <strong>2010</strong>, Pages 953-958<br />
Document Type : Article<br />
Keywords : Erythropoiesis; miRNA; Thalassemia<br />
<strong>Abstract</strong> : MicroRNAs (miRNAs) are negative regulators <strong>of</strong> gene expression that play an important role in hematopoiesis. Thalassemia, a<br />
defective globin synthesis leading to precipitate <strong>of</strong> excess unbound globins in red blood cell precursors, results in defective<br />
erythroid precursors and ineffective erythropoiesis. Expression pattern <strong>of</strong> miR-451, an erythroid-specific miRNA, was analyzed<br />
during differentiation <strong>of</strong> erythroid progenitors derived from normal and thalassemic peripheral blood CD34-positive cells, after<br />
14 days <strong>of</strong> culture. A biphasic expression with transient up-regulation <strong>of</strong> miRNA-451 on day 3 <strong>of</strong> cultures was observed during<br />
thalassemic erythroid differentiation. In contrast, the expression pattern <strong>of</strong> the miR-451 in erythroid cells obtained from the<br />
other extravascular hemolytic anemia, i.e., hereditary spherocytosis patients showed no transient up-regulation <strong>of</strong> miR-451 on<br />
day 3 <strong>of</strong> cultures. Our results suggest that early erythroid progenitors in β-thalassemia have a dysregulated miRNA-451<br />
expression program, and analysis <strong>of</strong> microRNA is a relevant approach to determine abnormalities <strong>of</strong> erythropoiesis. © <strong>2010</strong><br />
Springer-Verlag.<br />
No.1443<br />
Author (s) : Syhavong, B., Rasachack, B., Smythe, L., Rolain, J.-M., Roque-Afonso, A.-M., Jenjaroen, K., Soukkhaserm, V., Phongmany, S.,<br />
Phetsouvanh, R., Soukkhaserm, S., Thammavong, T., Mayxay, M., Blacksell, S.D., Barnes, E., Parola, P., Dussaix, E., Raoult, D.,<br />
Humphreys, I., Klenerman, P., White, N.J., Newton, P.N.<br />
524
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : The infective causes <strong>of</strong> hepatitis and jaundice amongst hospitalised patients in Vientiane, Laos<br />
Source : Transactions <strong>of</strong> the Royal Society <strong>of</strong> Tropical Medicine and Hygiene, Volume 104, Issue 7, July <strong>2010</strong>, Pages 475-483<br />
Document Type : Article<br />
Keywords : Hepatitis E virus; Jaundice; Laos; Leptospirosis; Orientia tsutsugamushi; Rickettsia typhi<br />
<strong>Abstract</strong> : There is little information on the diverse infectious causes <strong>of</strong> jaundice and hepatitis in the Asiatic tropics. Serology (hepatitis A,<br />
B, C and E, leptospirosis, dengue, rickettsia), antigen tests (dengue), PCR assays (hepatitis A, C and E) and blood cultures<br />
(septicaemia) were performed on samples from 392 patients admitted with jaundice or raised transaminases (≥×3) to Mahosot<br />
Hospital, Vientiane, Laos over 3 years. Conservative definitions suggested diagnoses <strong>of</strong> dengue (8.4%), rickettsioses (7.3%),<br />
leptospirosis (6.8%), hepatitis B (4.9%), hepatitis C (4.9%), community-acquired septicaemia (3.3%) and hepatitis E (1.6%).<br />
Although anti-hepatitis A virus (HAV) IgM antibody results suggested that 35.8% <strong>of</strong> patients had acute HAV infections, anti-HAV<br />
IgG antibody avidity and HAV PCR suggested that 82% had polyclonal activation and not acute HAV infections. Scrub typhus,<br />
murine typhus or leptospirosis were present in 12.8% <strong>of</strong> patients and were associated with meningism and relatively low AST<br />
and ALT elevation. These patients would be expected to respond to empirical doxycycline therapy which, in the absence <strong>of</strong><br />
virological diagnosis and treatment, may be an appropriate cost-effective intervention in Lao patients with jaundice/hepatitis.<br />
© <strong>2010</strong> Royal Society <strong>of</strong> Tropical Medicine and Hygiene.<br />
No.1444<br />
Author (s) : Tabsan, N., Wirasate, S., Suchiva, K.<br />
Title : Abrasion behavior <strong>of</strong> layered silicate reinforced natural rubber<br />
Source : Wear, Volume 269, Issue 5-6, July <strong>2010</strong>, Pages 394-404<br />
Document Type : Article<br />
Keywords : Abrasion; Layered silicate; Montmorillonite; Natural rubber<br />
<strong>Abstract</strong> : The abrasion resistance and abrasion behavior <strong>of</strong> Montmorillonite (MMT) filled natural rubber (NR) vulcanizates were<br />
investigated. Cure characteristic results indicated that both pristine Montmorillonite (Na+-MMT) and organically modified<br />
Montmorillonite (OMMT) acted as accelerator for the vulcanization reaction <strong>of</strong> NR. The abrasion resistance <strong>of</strong> NR was<br />
improved by the addition <strong>of</strong> either Na+-MMT or OMMT. OMMT filled NR composites showed superior abrasion resistance than<br />
those <strong>of</strong> Na+-MMT filled NR composites due to smaller size particles and better dispersion <strong>of</strong> OMMT in the NR matrix. DIN<br />
volume loss <strong>of</strong> NR was reduced by 91% when only 3phr <strong>of</strong> OMMT was added. An addition <strong>of</strong> MMT resulted in the formation<br />
<strong>of</strong> ridges on the abraded surfaces <strong>of</strong> NR. Finer ridges with narrowed spacing on the abraded surfaces corresponded to better<br />
abrasion resistance <strong>of</strong> NR composites. An addition <strong>of</strong> silane (Si69) resulted in the reduction <strong>of</strong> Na+-MMT particle size with the<br />
improvement <strong>of</strong> the abrasion resistance <strong>of</strong> the NR/Na+-MMT composites and better defined ridges on the abraded surfaces.<br />
Particle size and dispersion <strong>of</strong> OMMT remained the same by the addition <strong>of</strong> Si69, thus, the abrasion resistance, abrasion<br />
pattern and mechanical properties were not affected. © <strong>2010</strong> Elsevier B.V.<br />
No.1445<br />
Author (s) : Taher, A.; Viprakasit, V.; Porter, J.; Kattamis, A.; Lawniczek, T.; Pereno, R.; Schoenborn-Kellenberger, O.; Cappellini, M.D.<br />
Title : FACTORS ASSOCIATED WITH IRON ACCUMULATION IN NON-TRANSFUSION-DEPENDENT THALASSAEMIA SYNDROMES: BASELINE<br />
DATA FROM MULTICENTER INTERNATIONAL DEFERASIROX STUDY (THALASSA)<br />
Source : HAEMATOLOGICA-THE HEMATOLOGY JOURNAL, Volume: 95, Supplement: 2, Pages: 707-707, Meeting <strong>Abstract</strong>: 1827,<br />
Published: JUN <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1446<br />
Author (s) : Takeuchi, R., Lawpoolsri, S., Imwong, M., Kobayashi, J., Kaewkungwal, J., Pukrittayakamee, S., Puangsa-Art, S., Thanyavanich, N.,<br />
Maneeboonyang, W., Day, N.P., Singhasivanon, P.<br />
Title : Directly-observed therapy (DOT) for the radical 14-day primaquine treatment <strong>of</strong> Plasmodium vivax malaria on the Thai-<br />
Myanmar border<br />
Source : Malaria Journal, Volume 9, Issue 1, <strong>2010</strong>, Article number 308<br />
Document Type : Article<br />
525
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : chloroquine, primaquine, antimalarial agent; absence <strong>of</strong> side effects, adolescent, adult, article, child, clinical trial, controlled<br />
clinical trial, controlled study, directly observed therapy, disease duration, drug dose increase, drug efficacy, drug self<br />
administration, female, fever, follow up, genotype, human, infection risk, intermethod comparison, major clinical<br />
study, malaria, male, mixed infection, Myanmar, patient compliance, Plasmodium falciparum, Plasmodium vivax, preschool<br />
child, randomized controlled trial, reinfection, school child, Thailand, Plasmodium vivax malaria, recurrent disease, treatment<br />
outcome; Adolescent, Antimalarials, Child, Child, Preschool, Chloroquine, Directly Observed Therapy, Female, Follow-Up<br />
Studies, Humans, Malaria, Vivax, Male, Myanmar, Primaquine, Recurrence, Thailand, Treatment Outcome<br />
<strong>Abstract</strong> : Background. Plasmodium vivax has a dormant hepatic stage, called the hypnozoite, which can cause relapse months after the<br />
initial attack. For 50 years, primaquine has been used as a hypnozoitocide to radically cure P. vivax infection, but major<br />
concerns remain regarding the side-effects <strong>of</strong> the drug and adherence to the 14-day regimen. This study examined the<br />
effectiveness <strong>of</strong> using the directly-observed therapy (DOT) method for the radical treatment <strong>of</strong> P. vivax malaria infection, to<br />
prevent reappearance <strong>of</strong> the parasite within the 90-day follow-up period. Other potential risk factors for the reappearance <strong>of</strong><br />
P. vivax were also explored. Methods. A randomized trial was conducted from May 2007 to January 2009 in a low malaria<br />
transmission area along the Thai-Myanmar border. Patients aged 3 years diagnosed with P. vivax by microscopy, were<br />
recruited. All patients were treated with the national standard regimen <strong>of</strong> chloroquine for three days followed by primaquine<br />
for 14 days. Patients were randomized to receive DOT or self-administered therapy (SAT). All patients were followed for three<br />
months to check for any reappearance <strong>of</strong> P. vivax. Results. Of the 216 patients enrolled, 109 were randomized to DOT and<br />
107 to SAT. All patients recovered without serious adverse effects. The vivax reappearance rate was significantly lower in the<br />
DOT group than the SAT group (3.4/10,000 person-days vs. 13.5/10,000 person-days, p = 0.021). Factors related to the<br />
reappearance <strong>of</strong> vivax malaria included inadequate total primaquine dosage received (< 2.75 mg/kg), duration <strong>of</strong> fever 2 days<br />
before initiation <strong>of</strong> treatment, parasite count on admission 10,000/νl, multiple P. vivax-genotype infection, and presence <strong>of</strong> P.<br />
falciparum infection during the follow-up period. Conclusions. Adherence to the 14-day primaquine regimen is important for<br />
the radical cure <strong>of</strong> P. vivax malaria infection. Implementation <strong>of</strong> DOT reduces the reappearance rate <strong>of</strong> the parasite, and may<br />
subsequently decrease P. vivax transmission in the area. © <strong>2010</strong> Takeuchi et al; licensee BioMed Central Ltd.<br />
No.1447<br />
Author (s) : Tammawong, S., Ninomiya, S., Kawasaki, H., Boonchird, C., Sumpradit, T.<br />
Title : Millerozyma phetchabunensis sp. nov., a novel ascomycetous yeast species isolated from Nam Nao forest soil in Thailand,<br />
and the transfer <strong>of</strong> Pichia koratensis to the genus Millerozyma<br />
Source : Journal <strong>of</strong> General and Applied Microbiology, Volume 56, Issue 1, 26 March <strong>2010</strong>, Pages 37-42<br />
Document Type : Article<br />
Keywords : D1/D2 domain; Internal transcribed spacer; Millerozyma; Millerozyma phetchabunensis sp. nov.; Novel yeast species<br />
<strong>Abstract</strong> : A total <strong>of</strong> 4 strains (DR2-4, DW2-1, DS2-1 and DS3-1) <strong>of</strong> undescribed ascomycetous yeast, isolated from Nam Nao forest soil,<br />
were identified as a novel species in the genus Millerozyma. The D1/D2 sequences <strong>of</strong> the strains differed from the closely<br />
related species Millerozyma acaciae and Pichia koratensis by 1.2% (7 nucleotide substitutions) and 1.4% (8 nucleotide<br />
substitutions). Phenotypically, all the novel strains were identical, but differed from M. acaciae and P. koratensis by a variety<br />
<strong>of</strong> phenotypic characteristics. Based on phenotypic and phylogenetic data, these four yeast strains were assigned to a single<br />
novel species in the genus Millerozyma and the name Millerozyma phetchabunensis sp. nov. is proposed. In addition, we also<br />
propose the transfer <strong>of</strong> P. koratensis, which was placed in the Millerozyma clade based on the analysis <strong>of</strong> the D1/ D2 and ITS<br />
sequences, to the genus Millerozyma as M. koratensis comb. nov.<br />
No.1448<br />
Author (s) : Tan, P.T., Heiny, A.T., Miotto, O., Salmon, J., Marques Jr., E.T.A., Lemonnier, F., August, J.T.<br />
Title : Conservation and diversity <strong>of</strong> influenza A H1N1 HLA-restricted T cell epitope candidates for epitope-based vaccines<br />
Source : PLoS ONE, Volume 5, Issue 1, 18 January <strong>2010</strong>, Article number e8754<br />
Document Type : Article<br />
Keywords : alanylprolylisoleucylmethionylphenylalanylseryl<br />
asparaginyllysylmethionylalanylarginylleucylglycyllysylglycyltyrosylmethionine, arginylglutaminylthreonyltyrosylaspartyltryptop<br />
hyl threonylleucylasparaginylarginylasparaginylglutaminylprolyl<br />
alanylalanylthreonylalanine, arginylmethionylisoleucylglycylglycylisoleucylglycyl<br />
arginylphenylalanyltyrosylisoleucylglutaminylmethionylcysteinyl<br />
threonylglutamylleucine, asparaginylglutaminylprolylglutamyltryptophylphenyl<br />
526
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
alanylarginylasparaginylisoleucylleucylserylisoleucylalanylprolyl<br />
isoleucylmethionylphenylalanine, aspartyllysylleucylthreonylglutaminylglycylarginyl<br />
glutaminylthreonyltyrosylaspartyltryptophylthreonylleucyl<br />
asparaginylarginylasparaginylglutaminylprolylalanylalanylthreonylalanine, epitope, gamma<br />
interferon, glutamylarginylarginylasparaginyllysyltyrosylleucyl<br />
glutamylglutamylhistidylprolylserylalanylglycyllysylaspartylproline, glutamylvalylhistidylisoleucyltyrosyltyrosylleucyl<br />
glutamyllysylalanylasparaginyllysylisoleucyllysylserylglutamyllysine, glycylarginylphenylalanyltyrosylisoleucylglutaminyl<br />
methionylcysteinylthreonylglutamylleucyllysylleucylasparaginyl aspartyltyrosylglutamic<br />
acid, glycyltyrosylthreonylmethionylaspartylthreonylvalyl<br />
tryptophylarginylthreonylhistidylglutaminylvalylserylglutamylarginylglycine, histidylglutamylasparaginylarginylmethionylvalylleu<br />
cyl alanylserylthreonylthreonylalanyllysylalanylmethionylglutamylglutamine, HLA antigen class 1, HLA antigen class<br />
2, leucylalanylserylthreonylthreonylalanyllysylalanylmethionyl glutamylglutaminylmethionylalanylglycylserylserylglutamic<br />
acid, leucylglutamyllysylalanylasparaginyllysylisoleucyllysylseryl<br />
glutamyllysylthreonylhistidylisoleucylhistidylisoleucylphenylalanine, leucylglutamylvalylcysteinylphenylalanylmethionyl<br />
tyrosylserylaspartylphenylalanylhistidylphenylalanylisoleucyl<br />
asparaginylglutamylglutaminylglycine, leucylthreonylhistidylisoleucylmethionylisoleucyl<br />
tryptophylhistidylserylasparaginylleucylasparaginylaspartyl<br />
threonylthreonyltyrosylglutamine, lysylarginyltyrosylserylglutamylglutaminylmethionyl<br />
glutamylthreonylaspartylglycylglutamylarginylglutaminyl<br />
asparaginylalanylthreonine, lysyltryptophylvalylarginylglutamylleucylvalylleucyltyrosyl<br />
aspartyllysylglutamylglutamylisoleucylarginylarginylisoleucine, proteome, serylalanylglycylglutaminylisoleucylserylthreonyl<br />
glutaminylprolylthreonylphenylalanylserylvalylglutaminyl<br />
arginylasparaginylleucine, serylarginylprolylmethionylphenylalanylleucyltyrosylvalyl<br />
arginylthreonylasparaginylglycylthreonylseryllysylisoleucyllysine, serylhistidylglycylthreonylglycylthreonylglycyltyrosyl<br />
threonylmethionylaspartylthreonylvalylasparaginylarginylthreonylhistidine, threonylasparaginylprolylleucylisoleucylarginylhistid<br />
yl<br />
glutamylasparaginylarginylmethionylvalylleucylalanylserylthreonylthreonine, threonylglutaminylprolylthreonylphenylalanylser<br />
ylvalyl glutaminylarginylasparaginylleucylprolylphenylalanyl aspartyllysylthreonylthreonine, unclassified drug, unindexed<br />
drug, valylleucyltyrosylaspartyllysylglutamylglutamylisoleucyl<br />
arginylarginylisoleucyltryptophylarginylglutaminylalanylasparaginylasparagine, virus protein, virus vaccine, influenza vaccine;<br />
allele, amino acid sequence, animal experiment, animal tissue, antigenic variation, article, avian influenza, cluster<br />
analysis, comparative study, enzyme linked immunospot assay, HLA system, human, immunization, Influenza virus<br />
A, Influenza virus A H1N1, Influenza virus A H2N2, Influenza virus A H3N2, Influenza virus A<br />
H5N1, mouse, nonhuman, nucleotide sequence, T lymphocyte, transgenic mouse, virus<br />
strain, animal, chemistry, immunology, molecular genetics; Aves, Influenza A virus, Mus musculus, Orthomyxoviridae; Amino<br />
Acid Sequence, Animals, Epitopes, Humans, Influenza A Virus, H1N1 Subtype, Influenza Vaccines, Mice, Mice,<br />
Transgenic, Molecular Sequence Data, T-Lymphocytes<br />
<strong>Abstract</strong> : Background: The immune-related evolution <strong>of</strong> influenza viruses is exceedingly complex and current vaccines against influenza<br />
must be reformulated for each influenza season because <strong>of</strong> the high degree <strong>of</strong> antigenic drift among circulating influenza<br />
strains. Delay in vaccine production is a serious problem in responding to a pandemic situation, such as that <strong>of</strong> the current<br />
H1N1 strain. Immune escape is generally attributed to reduced antibody recognition <strong>of</strong> the viral hemagglutinin and<br />
neuraminidase proteins whose rate <strong>of</strong> mutation is much greater than that <strong>of</strong> the internal non-structural proteins. As a possible<br />
alternative, vaccines directed at T cell epitope domains <strong>of</strong> internal influenza proteins, that are less susceptible to antigenic<br />
variation, have been investigated. Methodology/Principal Findings: HLA transgenic mouse strains expressing HLA class I A*0201,<br />
A*2402, and B*0702, and class II DRB1*1501, DRB1*0301 and DRB1*0401 were immunized with 196 influenza H1N1 peptides<br />
that contained residues <strong>of</strong> highly conserved proteome sequences <strong>of</strong> the human H1N1, H3N2, H1N2, H5N1, and avian<br />
influenza A strains. Fifty-four (54) peptides that elicited 63 HLA-restricted peptide-specific T cell epitope responses were<br />
identified by IFN-γ ELISpot assay. The 54 peptides were compared to the 2007-2009 human H1N1 sequences for selection <strong>of</strong><br />
sequences in the design <strong>of</strong> a new candidate H1N1 vaccine, specifically targeted to highly-conserved HLA-restricted T cell<br />
epitopes. Conclusions/Significance: Seventeen (17) T cell epitopes in PB1, PB2, and M1 were selected as vaccine targets based<br />
on sequence conservation over the past 30 years, high functional avidity, non-identity to human peptides, clustered<br />
527
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
localization, and promiscuity to multiple HLA alleles. These candidate vaccine antigen sequences may be applicable to any<br />
avian or human influenza A virus. © <strong>2010</strong> Tan et al.<br />
No.1449<br />
Author (s) : Tan, T.-C., Pitsawong, W., Wongnate, T., Spadiut, O., Haltrich, D., Chaiyen, P., Divne, C.<br />
Title : H-bonding and positive charge at the N(5)/O(4) locus are critical for covalent flavin attachment in Trametes pyranose 2oxidase<br />
Source : Journal <strong>of</strong> Molecular Biology, Volume 402, Issue 3, September <strong>2010</strong>, Pages 578-594<br />
Document Type : Article<br />
Keywords : Flavinylation; Flavoprotein oxidase; GMC oxidoreductase; Pyranose 2-oxidase; Sulfite adduct<br />
<strong>Abstract</strong> : Flavoenzymes perform a wide range <strong>of</strong> redox reactions in nature, and a subclass <strong>of</strong> flavoenzymes carry covalently bound<br />
c<strong>of</strong>actor. The enzyme-flavin bond helps to increase the flavin's redox potential to facilitate substrate oxidation in several<br />
oxidases. The formation <strong>of</strong> the enzyme-flavin covalent bond-the flavinylation reaction-has been studied for the past 40 years.<br />
For the most advocated mechanism <strong>of</strong> autocatalytic flavinylation, the quinone methide mechanism, appropriate stabilization<br />
<strong>of</strong> developing negative charges at the flavin N(1) and N(5) loci is crucial. Whereas the structural basis for stabilization at N(1) is<br />
relatively well studied, the structural requisites for charge stabilization at N(5) remain less clear. Here, we show that<br />
flavinylation <strong>of</strong> histidine 167 <strong>of</strong> pyranose 2-oxidase from Trametes multicolor requires hydrogen bonding at the flavin<br />
N(5)/O(4) locus, which is <strong>of</strong>fered by the side chain <strong>of</strong> Thr169 when the enzyme is in its closed, but not open, state. Moreover,<br />
our data show that additional stabilization at N(5) by histidine 548 is required to ensure high occupancy <strong>of</strong> the histidyl-flavin<br />
bond. The combination <strong>of</strong> structural and spectral data on pyranose 2-oxidase mutants supports the quinone methide<br />
mechanism. Our results demonstrate an elaborate structural fine-tuning <strong>of</strong> the active site to complete its own formation that<br />
couples efficient holoenzyme synthesis to conformational substates <strong>of</strong> the substrate-recognition loop and concerted<br />
movements <strong>of</strong> side chains near the flavinylation ligand. © <strong>2010</strong> Elsevier Ltd.<br />
No.1450<br />
Author (s) : Tananuwong, K., Lertsiri, S.<br />
Title : Changes in volatile aroma compounds <strong>of</strong> organic fragrant rice during storage under different conditions<br />
Source : Journal <strong>of</strong> the Science <strong>of</strong> Food and Agriculture, Volume 90, Issue 10, 15 August <strong>2010</strong>, Pages 1590-1596<br />
Document Type : Article<br />
Keywords : Organic fragrant rice; Packaging; SPME/GC/MS; Storage temperature; Volatile compounds<br />
<strong>Abstract</strong> : BACKGROUND: Aroma <strong>of</strong> fragrant rice greatly affects palatability as well as consumer acceptability. Loss <strong>of</strong> desirable volatile<br />
compounds such as 2-acetyl-1-pyrroline (2-AP) and increase in volatile lipid oxidation products in aged rice have been<br />
reported. The aim <strong>of</strong> this study was to monitor the changes in key volatile compounds <strong>of</strong> organic red fragrant rice cv. Hom<br />
Daeng during storage. Effects <strong>of</strong> packaging material, storage temperature and time were evaluated using solid phase<br />
microextraction/gas chromatography/mass spectrometry. RESULTS: Thirteen key volatile compounds including ten lipid<br />
oxidation productsweremonitored. For samples vacuum packed in Nylon/LLDPE pouches at ambient temperature, significant<br />
increases in hexanal, 2-pentylfuran, 1-octanol and 4-vinyl guaiacol and significant decreases in 2-AP and geranyl acetone were<br />
found after the second month (P ≤ 0.05). Vacuum packing in OPP/Al/LLDPE pouchesor storage at 15 ° Cbetter retarded the<br />
formation<strong>of</strong> volatile lipid oxidationproducts andgreater retained desirable odorants, including 2-AP. However, accumulation <strong>of</strong><br />
lipid oxidation products and 4-vinyl guaiacol was apparent after the sixth month under these storage conditions.<br />
CONCLUSION: A greater extent and higher rate <strong>of</strong> undesirable changes in volatile compounds were found in samples stored<br />
under Nylon/LLDPE/ambient temperature condition. Nevertheless, this condition is acceptable for the retail trade <strong>of</strong> organic<br />
rice in Thailand. Storage conditions using reduced temperature or better packaging materials may be more appropriate for<br />
exported rice or superior-grade fragrant rice to better maintain the desirable rice aroma. © <strong>2010</strong> Society <strong>of</strong> Chemical Industry.<br />
No.1451<br />
Author (s) : Tanasoontornrerk, A., Wasinrat, J., Siriapisith, T., Slisatkorn, W.<br />
Title : CT angiography evaluation <strong>of</strong> endoleak after thoracic endovascular aortic repair in thoracic aortic aneurysm<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 9, September <strong>2010</strong>, Pages 1050-1057<br />
Document Type : Article<br />
Keywords : Aortic aneurysm; CT angiography; Endovascular aortic repair; Multidetector row CT<br />
528
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : Objective: Analyze the incidence and findings <strong>of</strong> endoleak after thoracic endovascular aortic repair by using CT angiography.<br />
Material and method: Between August 2006 and December 2008, 68 patients diagnosed with thoracic aortic aneurysm<br />
underwent thoracic endovascular aortic repair and were included in the present study. The patients were 47 men and 21<br />
women, with a mean age <strong>of</strong> 69.± 9.4 years old. Thoraco-abdominal CT angiographic images (64-slice MDCT) after operation <strong>of</strong><br />
68 patients were retrospectively reviewed to evaluate incidence <strong>of</strong> endoleak and classify findings <strong>of</strong> endoleak. Results:<br />
Endoleaks were detected in 26 patients (38.2%). There were type I endoleaks in three cases (11.5%), type II endoleaks in 22<br />
cases (84.6%), and type III endoleaks in one case (3.9%). Type II endoleaks were detected as peritubular collection, mostly<br />
located at periphery <strong>of</strong> the aneurysm. Eleven cases (50%) <strong>of</strong> type II endoleaks were supplied by left subclavian artery.<br />
Twenty patients who had completed 1, 3, and 6 months follow-up CT angiography were selected for further evaluation <strong>of</strong><br />
changing in size <strong>of</strong> aneurysm. The measurement <strong>of</strong> the thoracic aneurysm showed no decreasing <strong>of</strong> the maximum length <strong>of</strong><br />
diameter and volume <strong>of</strong> the aneurysmal sac in endoleak group. Conclusion: Follow-up CT angiography is useful for detection<br />
and characterization <strong>of</strong> endoleak after endovascular aortic repair <strong>of</strong> thoracic aneurysm. Most <strong>of</strong> type II endoleaks show<br />
peritubular (collection) shape and locate at the periphery. Patients with endoleak after thoracic endovascular aortic repair<br />
tend to continue to have sac expansion.<br />
No.1452<br />
Author (s) : Tanboon, J.<br />
Title : Melanocytic matricoma: a case report with review literature<br />
Source : HISTOPATHOLOGY, Volume: 57, Supplement: 1, Pages: 66-66, Meeting <strong>Abstract</strong>: 181, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1453<br />
Author (s) : Tanboon, J.; Uiprasertkul, M.; Leurmsamran, P.<br />
Title : Histiocytoid carcinoma <strong>of</strong> the eyelid: a case report with review literatures<br />
Source : HISTOPATHOLOGY, Volume: 57, Supplement: 1, Pages: 210-210, Meeting <strong>Abstract</strong>: 584, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1454<br />
Author (s) : Tandhavanant, S., Thanwisai, A., Limmathurotsakul, D., Korbsrisate, S., Day, N.P., Peacock, S.J., Chantratita, N.<br />
Title : Effect <strong>of</strong> colony morphology variation <strong>of</strong> Burkholderia pseudomallei on intracellular survival and resistance to antimicrobial<br />
environments in human macrophages in vitro<br />
Source : BMC Microbiology, Volume 10, <strong>2010</strong>, Article number 303<br />
Document Type : Article<br />
Keywords : beta defensin 2, cathelicidin antimicrobial peptide LL 37, hydrogen peroxide, lact<strong>of</strong>errin, lysozyme, sodium<br />
nitrite, antiinfective agent, antimicrobial peptide LL-37, cathelicidin; antibacterial activity, article, bacterial growth, bacterial<br />
survival, bacterium colony, Burkholderia pseudomallei, controlled study, human, human<br />
cell, macrophage, morphology, morphotype, nonhuman, phenotype, strain difference, antibiotic resistance, cell strain<br />
U937, classification, cytology, drug effect, growth, development and aging, isolation and purification, melioidosis, microbial<br />
viability, microbiology; Burkholderia pseudomallei; Anti-Bacterial Agents, Burkholderia pseudomallei, Cathelicidins, Drug<br />
Resistance, Bacterial, Humans, Hydrogen Peroxide, Macrophages, Melioidosis, Microbial Viability, U937 Cells<br />
<strong>Abstract</strong> : Background. Primary diagnostic cultures from patients with melioidosis demonstrate variation in colony morphology <strong>of</strong> the<br />
causative organism, Burkholderia pseudomallei. Variable morphology is associated with changes in the expression <strong>of</strong> a range<br />
<strong>of</strong> putative virulence factors. This study investigated the effect <strong>of</strong> B. pseudomallei colony variation on survival in the human<br />
macrophage cell line U937 and under laboratory conditions simulating conditions within the macrophage milieu. Isogenic<br />
colony morphology types II and III were generated from 5 parental type I B. pseudomallei isolates using nutritional limitation.<br />
Survival <strong>of</strong> types II and III were compared with type I for all assays. Results. Morphotype was associated with survival in the<br />
presence <strong>of</strong> H2O2and antimicrobial peptide LL-37, but not with susceptibility to acid, acidified sodium nitrite, or resistance to<br />
lysozyme, lact<strong>of</strong>errin, human neutrophil peptide-1 or human beta defensin-2. Incubation under anaerobic conditions was a<br />
529
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
strong driver for switching <strong>of</strong> type III to an alternative morphotype. Differences were noted in the survival and replication <strong>of</strong><br />
the three types following uptake by human macrophages, but marked strain-to strain-variability was observed. Uptake <strong>of</strong> type<br />
III alone was associated with colony morphology switching. Conclusions. Morphotype is associated with phenotypes that alter<br />
the ability <strong>of</strong> B. pseudomallei to survive in adverse environmental conditions. © <strong>2010</strong> Tandhavanant et al.<br />
No.1455<br />
Author (s) : Tangnararatchakit, K., Chuansumrit, A., Chaiyaratana, W., Lertwongrath, S., Gajaseeni, N., Udomchaisakul, R., O-Prasertsawat, P.,<br />
Yoksan, S.<br />
Title : Excessive menstrual bleeding in adolescents with dengue infection<br />
Source : Pediatric Infectious Disease Journal, Volume 29, Issue 1, January <strong>2010</strong>, Pages 92-93<br />
Document Type : Letter<br />
Keywords : conjugated estrogen, norethisterone, oral contraceptive agent; adolescent, child, controlled<br />
study, dengue, female, human, letter, major clinical study, male, menstruation, priority journal, school child;<br />
Adolescent, Dengue, Female, Humans, Male, Menstruation<br />
<strong>Abstract</strong> : –<br />
No.1456<br />
Author (s) : Tangprasittipap, A., Tiensuwan, M., Withyachumnarnkul, B.<br />
Title : Characterization <strong>of</strong> candidate genes involved in growth <strong>of</strong> black tiger shrimp Penaeus monodon<br />
Source : Aquaculture, Volume 307, Issue 1-2, September <strong>2010</strong>, Pages 150-156<br />
Document Type : Article<br />
Keywords : Body weight; Cyclophilin; EST-library; Optic lobe; Penaeus monodon<br />
<strong>Abstract</strong> : The optic lobe <strong>of</strong> the female-shrimp eyestalk was selected as the target tissue for suppressive subtractive hybridization (SSH)<br />
because it is a place for synthesis <strong>of</strong> many hormones and peptides/enzymes involved in molting and growth. SSH was<br />
performed to screen genes differentially expressed in the optic lobe between large female (LF; body weight>90 percentile<br />
<strong>of</strong> weight distribution curve) and small female (SF; body weight<10 percentile). A total <strong>of</strong> 426 recombinant clones were<br />
obtained from the two directions. After sequencing and analysis less than 30% <strong>of</strong> the resulting expressed sequence tag (EST)<br />
exhibited high homology to known records at GenBank (BlastX with E-value<10-4) indicating little available molecular<br />
information on the optic lobe. Among the EST with relatively high homology to GenBank records, 5 candidate ESTs had<br />
homology to records for genes involved in cell differentiation/proliferation, cell cycle and hormone processing. These<br />
included genes resembling cyclophilin, cyclophilin A, fibrillarin, SPARC and PC2. These candidate ESTs were selected to<br />
confirm the reproducibility <strong>of</strong> the SSH data using semi-quantitative RT-PCR normalized with EF-1α. Pearson's correlation<br />
analysis confirmed that the index <strong>of</strong> relative cyclophilin, SPARC and fibrillarin-like expression was negatively correlated with<br />
body weight (p<0.05) and this supported the SSH data indicating their low relative expression in LF shrimp when compared<br />
to SF shrimp. The index <strong>of</strong> relative cyclophilin-like expression showed the highest correlation coefficient with body weight (r=-<br />
0.678). The relationship could be expressed by inverse fitted equation: body weight (g)=38.243/(index <strong>of</strong> relative cyclophilinlike<br />
expression). This fitted inverse model could predict the body weight <strong>of</strong> female shrimp with 91.6 % coefficient <strong>of</strong><br />
determination (R2) based on the index <strong>of</strong> relative cyclophilin-like expression in the optic lobe <strong>of</strong> female P. monodon. © <strong>2010</strong><br />
Elsevier B.V.<br />
No.1457<br />
Author (s) : Tangpukdee, N., Wai, K.M., Muangnoicharoen, S., Kano, S., Phophak, N., Tiemprasert, J., Krudsood, S., Wilairatana, P.<br />
Title : Indicators <strong>of</strong> fatal outcome in severe Plasmodium falciparum malaria: A study in a tertiary-care hospital in Thailand<br />
Source : Asian Pacific Journal <strong>of</strong> Tropical Medicine, Volume 3, Issue 11, November <strong>2010</strong>, Pages 855-859<br />
Document Type : Article<br />
Keywords : Fatal indicator; Plasmodium falciparum; Severe malaria; Tertiary care; Thailand<br />
<strong>Abstract</strong> : Objective: To illustrate the clinical features and investigate the indicators associated with a fatal outcome in adult patients<br />
with severe Plasmodium falciparum malaria admitted to the Hospital for Tropical Diseases, Bangkok, Thailand. Methods: We<br />
studied 202 adult malaria patients admitted to the Intensive Care Unit. A total <strong>of</strong> 43 clinical variables were identified by<br />
univariate and logistic regression analyses, to eliminate confounding factors. Results: Regarding the statistical methods, only 6<br />
variables-jaundice, cerebral malaria, metabolic acidosis, body mass index, initial respiratory rate, and white blood cell countwere<br />
significant indicators <strong>of</strong> death, with adjusted odds ratios (95% CI) <strong>of</strong> 15.2 (2.1-32.3), 4.3 (2.3-12.6), 3.3 (2.3-5.7), 2.4 (1.9-<br />
530
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
3.5), 2.2 (1.5-2.6), and 1.7 (1.2-3.1), respectively. Conclusion: Our study found that jaundice, cerebral malaria, metabolic<br />
acidosis, body mass index, initial respiratory rate and white blood cell count were indicators <strong>of</strong> fatal outcome in severe<br />
Plasmodium falciparum malaria. Further studies on the fatal indicators in severe malaria need to be compared with data from<br />
different geographical areas, to construct practical measures to address potentially fatal indicators in different settings. © <strong>2010</strong><br />
Hainan Medical College.<br />
No.1458<br />
Author (s) : Tangsudjai, S., Pudla, M., Limposuwan, K., Woods, D.E., Sirisinha, S., Utaisincharoen, P.<br />
Title : Involvement <strong>of</strong> the MyD88-independent pathway in controlling the intracellular fate <strong>of</strong> Burkholderia pseudomallei infection in<br />
the mouse macrophage cell line RAW 264.7<br />
Source : Microbiology and Immunology, Volume 54, Issue 5, May <strong>2010</strong>, Pages 282-290<br />
Document Type : Article<br />
Keywords : Burkholderia pseudomallei; Melioidosis; MyD88-independent pathway; TBK1<br />
<strong>Abstract</strong> : Burkholderia pseudomallei is a facultative intracellular Gram-negative bacterium which is capable <strong>of</strong> surviving and multiplying<br />
inside macrophages. B. pseudomallei strain SRM117, a LPS mutant which lacks the O-antigenic polysaccharide moiety, is more<br />
susceptible to macrophage killing during the early phase <strong>of</strong> infection than is its parental wild type strain (1026b). In this study,<br />
it was shown that the wild type is able to induce expression <strong>of</strong> genes downstream <strong>of</strong> the MyD88-dependent (iκbζ, il-6 and<br />
tnf-α), but not <strong>of</strong> the MyD88-independent (inos, ifn-βand irg-1), path ways in the mouse macrophage cell line RAW 264.7. In<br />
contrast, LPS mutant-infected macrophages were able to express genes downstream <strong>of</strong> both pathways. To elucidate the<br />
significance <strong>of</strong> activation <strong>of</strong> the MyD88-independent pathway in B. pseudomallei-infected macrophages, the expression <strong>of</strong><br />
TBK1, an essential protein in the MyD88-independent pathway, was silenced prior to the infection. The results showed that<br />
silencing the tbk1 expression interferes with the gene expression pr<strong>of</strong>ile in LPS mutant-infected macrophages and allows the<br />
bacteria to replicate intracellularly, thus suggesting that the MyD88-independent pathway plays an essential role in<br />
controlling intracellular survival <strong>of</strong> the LPS mutant. Moreover, exogenous IFNγ upregulated gene expression downstream <strong>of</strong><br />
the MyD88-independent pathway, and interfered with intracellular survival in both wild type and tbk1-knockdown<br />
macrophages infected with either the wild type or the LPS mutant. These results suggest that gene expression downstream <strong>of</strong><br />
the MyD88-independent pathway is essential in regulating the intracellular fate <strong>of</strong> B. pseudomallei, and that IFN-γ regulates<br />
gene expression through the TBK1-independent pathway. © <strong>2010</strong> The Societies and Blackwell Publishing Asia Pty Ltd.<br />
No.1459<br />
Author (s) : Tangwongsan, S., Rassameeroj, L.<br />
Title : Echo cancellation in VoIP with improved least square lattice method<br />
Source : World Review <strong>of</strong> Science, Technology and Sustainable Development, Volume 7, Issue 1-2, March <strong>2010</strong>, Pages 141-160<br />
Document Type : Article<br />
Keywords : Adaptive filter; Echo cancellation; Least square lattice; LSL; Voice over internet protocol; VoIP<br />
<strong>Abstract</strong> : In this work, an approach to echo cancellation in VoIP network based on the packet-based echo cancellation model was<br />
employed. In addition, a comparative validation was performed and then analysed specifically in the state <strong>of</strong> echo canceller<br />
as adaptive filtering was done by using three algorithms: normalised least mean square (NLMS), recursive least square (RLS)<br />
and least square lattice (LSL). Communication environments with three different echo strength levels: 20%, 50% and 70% <strong>of</strong><br />
far-end speech were simulated. The result shows that in terms <strong>of</strong> time computation, NLMS is the best, followed by LSL and<br />
RLS as the last. However, NLMS suffers from the measure <strong>of</strong> ERLE, as it falls to the third place, followed by LSL and RLS as<br />
the first. Then, taking them all into consideration, it could be concluded that LSL is the best among all for echo cancellation<br />
as verified in the experimental simulation. Copyright © <strong>2010</strong> Inderscience Enterprises Ltd.<br />
No.1460<br />
Author (s) : Tangwongsan, S., Suvacharakulton, B.<br />
Title : Realization <strong>of</strong> a high performance bilingual OCR system for Thai-English printed documents<br />
Source : Proceedings <strong>of</strong> the 6th <strong>International</strong> Conference on Natural Language Processing and Knowledge Engineering, NLP-KE <strong>2010</strong>,<br />
<strong>2010</strong>, Article number 5587781<br />
Document Type : Conference Paper<br />
Keywords : Bilingual OCR; Dictionary look-up; Language identification; Thai-English character recognition<br />
531
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : This paper presents a high performance bilingual OCR system for printed Thai and English text. With the complex nature <strong>of</strong><br />
both Thai and English languages, the first stage is to identify languages within different zones by using geometric properties for<br />
differentiation. The second stage is the process <strong>of</strong> character recognition, in which the technique developed includes a feature<br />
extractor and a classifier. In the feature extraction, the thinned character image is analyzed and categorized into groups. Next,<br />
the classifier will take in two steps <strong>of</strong> recognition: the coarse level, followed by the fine level with a guide <strong>of</strong> decision trees.<br />
As to obtain an even better result, the final stage attempts to make use <strong>of</strong> dictionary look-up as to check for accuracy<br />
improvement in an overall performance. For verification, the system is tested by a series <strong>of</strong> experiments with printed<br />
documents in 141 pages and over 280,000 characters, the result shows that the system could obtain an accuracy <strong>of</strong> 100% in<br />
Thai monolingual, 98.18% in English monolingual, and 99.85% in bilingual documents on the average. In the final stage with a<br />
dictionary look-up, the system could yield a better accuracy <strong>of</strong> improvement up to 99.98% in bilingual documents as<br />
expected. ©<strong>2010</strong> IEEE.<br />
No.1461<br />
Author (s) : Tangyuenyongwatana, P., Gritsanapan, W.<br />
Title : Quantitative analysis and toxicity determination <strong>of</strong> artifacts originated in a Thai traditional medicine Prasaplai<br />
Source : Pharmaceutical Biology, Volume 48, Issue 5, May <strong>2010</strong>, Pages 584-588<br />
Document Type : Article<br />
Keywords : Acute toxicity; Artifact formation; Cytotoxicity; Fatty acid ester; Prasaplai; Thai traditional medicine<br />
<strong>Abstract</strong> : Prasaplai is a Thai traditional medicine for relieving dysmenorrhea and adjusting the menstrual cycle. Three fatty acid esters,<br />
(E)-4-(3,4- dimethoxyphenyl)but-3-en-1-yl linoleate (1), (E)-4-(3,4-dimethoxyphenyl)but-3- en-1-yl oleate (2) and (E)-4-(3,4dimethoxyphenyl)but-3-en-1-yl<br />
palmitate (3) are formed during storage from the reaction <strong>of</strong> chemical components in two<br />
herbs, i.e., fatty acids in Nigella sativa (L.) (Ranunculaceae) and (E)-4-(3,4-dimethoxyphenyl)but-3-en-1-ol (compound D) in<br />
Zingiber cassumunar (Roxb.) (Zingiberaceae). The formations <strong>of</strong> these artifacts were monitored for 1 year and their amounts<br />
were analyzed by HPLC at certain periods <strong>of</strong> time. The results showed that artifact formation was saturated after 73 days <strong>of</strong><br />
storage. The amount <strong>of</strong> each artifact in the saturation period ranged from 3.93±0.06 to 4.30±0.18% w/w for compound 1,<br />
1.69±0.08 to 1.9±0.13% w/w for compound 2 and 0.09±0.003 to 0.1±0.005% w/w for compound 3. Cytotoxicity <strong>of</strong> the<br />
artifacts was evaluated using NCI-H187, KB, and BC cancer cell lines and found that the IC50 <strong>of</strong> all artifacts in all tests were<br />
higher than 20 μg/mL. For acute toxicity in mice, the LD50 <strong>of</strong> each artifact was more than 300mg/kg. © <strong>2010</strong> Informa UK Ltd.<br />
No.1462<br />
Author (s) : Tanmahasamut, P., Wongwananuruk, T.<br />
Title : Challenging regimen for long-term conservative treatment <strong>of</strong> endometrial adenocarcinoma in young women: A case report<br />
and review <strong>of</strong> the literature<br />
Source : Case Reports in Oncology, Volume 3, Issue 3, September <strong>2010</strong>, Pages 380-385<br />
Document Type : Review<br />
Keywords : Endometrial adenocarcinoma; Levonorgestrel intrauterine system; Polycystic ovary syndrome<br />
<strong>Abstract</strong> : A 20-year-old obese Thai woman with polycystic ovary syndrome and clinical stage I well-differentiated endometrial<br />
carcinoma denied surgical staging. Chest X-ray and magnetic resonance imaging <strong>of</strong> the whole abdomen suggested neither<br />
distance metastasis nor local invasion <strong>of</strong> the cancer. After 3 months <strong>of</strong> systemic progestin therapy with megestrol acetate<br />
(MA) 480 mg/day, the endometrial carcinoma persisted. The treatment was changed to a combination <strong>of</strong> levonorgestrel<br />
intrauterine system (LNG-IUS) and MA with a stepping-up from 160 to 480 mg/day. Complete remission was achieved at<br />
treatment month 9. Prevention <strong>of</strong> recurrence was provided using LNG-IUS plus MA 160 mg/day. Endometrial surveillance<br />
using trimonthly transvaginal ultrasonography and endometrial biopsy suggested no recurrence for at least 24 months after<br />
remission. © <strong>2010</strong> S. Karger AG, Basel.<br />
No.1463<br />
Author (s) : Tanomsing, N., Imwong, M., Theppabutr, S., Pukrittayakamee, S., Day, N.P.J., White, N.J., Snounou, G.<br />
Title : Accurate and sensitive detection <strong>of</strong> Plasmodium species in humans by use <strong>of</strong> the dihydr<strong>of</strong>olate reductase-thymidylate<br />
synthase linker region<br />
Source : Journal <strong>of</strong> Clinical Microbiology, Volume 48, Issue 10, October <strong>2010</strong>, Pages 3735-3737<br />
Document Type : Article<br />
532
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : dihydr<strong>of</strong>olate reductase, thymidylate synthase; article, diagnostic accuracy, human, nucleotide sequence, parasite<br />
identification, Plasmodium, polymerase chain reaction, priority journal, sensitivity analysis<br />
<strong>Abstract</strong> : A nested-PCR protocol based on the linker region <strong>of</strong> the Plasmodium dihydr<strong>of</strong>olate reductase-thymidylate synthase gene<br />
(dhfr-ts) was developed. This provides highly sensitive specific detection and identification <strong>of</strong> the five parasite species that<br />
infect humans. Copyright © <strong>2010</strong>, American Society for Microbiology. All Rights Reserved.<br />
No.1464<br />
Author (s) : Tanpantree, S., Opaprakasit, P., Loykulnant, S., Kangwansupamonkon, W., Tangboriboonrat, P.<br />
Title : Nanocapsules embedded in natural rubber latex gloves<br />
Source : Journal <strong>of</strong> Applied Polymer Science, Volume 117, Issue 3, 1 August <strong>2010</strong>, Pages 1798-1803<br />
Document Type : Article<br />
Keywords : Elastomers; Films; Morphology; Rubber; Surfaces<br />
<strong>Abstract</strong> : To prepare medical gloves containing disinfectant agent, poly(methyl acrylate) (PMA) with M̄w <strong>of</strong> 550 K was synthesized via<br />
the iniferter technique and then used for encapsulating disinfectant agent, i.e., chlorhexidine digluconate (CHD), droplets. The<br />
CHD-PMA nanocapsules suspended in a sodium dodecyl sulfate aqueous solution having 93% encapsulation efficiency were<br />
successfully embedded between the outermost and inner layers <strong>of</strong> γ-radiation vulcanized natural rubber (RVNR) latex films<br />
by the coagulant dipping process. A RVNR/CHD-PMA nanocapsules/RVNR three-layer structure was revealed by the contact<br />
angle measurement, ATR-FTIR and SEM. Both the tensile strength and elongation at break <strong>of</strong> the three-layer film were also<br />
measured. © <strong>2010</strong> Wiley Periodicals, Inc.<br />
No.1465<br />
Author (s) : Tanpantree, S., Opaprakasit, P., Polpanich, D., Smanmoo, S., Tangboriboonrat, P.<br />
Title : Polymeric disinfectant nanocapsules: Effect <strong>of</strong> molecular weight <strong>of</strong> poly(methyl acrylate)<br />
Source : Journal <strong>of</strong> Biomedical Nanotechnology, Volume 6, Issue 4, August <strong>2010</strong>, Pages 385-390<br />
Document Type : Article<br />
Keywords : Disinfectant agent; Iniferter; Nanocapsule; Nanoparticle<br />
<strong>Abstract</strong> : Poly(methyl acrylate) (PMA) <strong>of</strong> various weight average molecular weights (M̄w) were synthesized via the iniferter route and<br />
applied for the encapsulation <strong>of</strong> disinfectant agent (chlorhexidine digluconate; CHD) by using the controlled precipitation<br />
technique. The encapsulation efficiency <strong>of</strong> CHD-PMA nanocapsules suspended in cyclohexane increased from 70 to 100%<br />
when the M̄w <strong>of</strong> PMA increased from 40 K to 550 K. After redispersing the capsules into 0.5% w/v <strong>of</strong> sodium dodecyl sulphate<br />
aqueous solution, the maximum content <strong>of</strong> CHD in the nanocapsules remained >90%. Transmission electron micrographs<br />
showed the spherical nanocapsule with continuous intact shell. Copyright © <strong>2010</strong> American Scientific Publishers. All rights<br />
reserved.<br />
No.1466<br />
Author (s) : Tantai, N.; Chaikledkaew, U.; Werayingyong, P.; Teerawattananon, Y.<br />
Title : A SYSTEMATIC REVIEW AND META-ANALYSIS OF LITERATURE ASSESSING CLINICAL EFFICACY OF TREATMENTS FOR PATIENTS<br />
WITH HBEAG-POSITIVE CHRONIC HEPATITIS B<br />
Source : VALUE IN HEALTH, Volume: 13, Issue: 7, Pages: A531-A532, Published: NOV <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1467<br />
Author (s) : Tantemsapya, N.; Melin-Aldana, H.; Wang, D.; Superina, R.<br />
Title : DIGITAL MORPHOMETRIC STUDY OF IDIOPATHIC EXTRAHEPATIC PORTAL VEIN THROMBOSIS CORRELATED TO CLINICAL<br />
OUTCOME OF MESENTERIC TO LEFT PORTAL VEIN BYPASS IN PEDIATRIC PATIENTS<br />
Source : JOURNAL OF HEPATOLOGY, Volume: 52, Supplement: 1, Pages: S437-S437, Published: <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
533
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.1468<br />
Author (s) : Tantichaiyakul, P., Preutthipan, A.<br />
Title : Ketotifen versus inhaled budesonide for controlling childhood asthma<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 5, May <strong>2010</strong>, Pages 541-549<br />
Document Type : Article<br />
Keywords : Asthma; Budesonide; Children; Ketotifen; Randomized control trial<br />
<strong>Abstract</strong> : Background: <strong>International</strong> asthma guideline recommends inhaled corticosteroids therapy for children <strong>of</strong> all ages as the first<br />
controller. However, in some less developed parts <strong>of</strong> the world, ketotifen, an old inexpensive medicine with antihistaminic<br />
and anti-allergic reactions, has been found to be the most favored prophylactic agents. Objective: To compare the efficacy<br />
and safety <strong>of</strong> ketotifen and inhaled budesonide in asthmatic children aged 8 months to 14 years at Banpong Hospital, located<br />
80km south from Bangkok. Material and Method: Children who had been admitted with acute asthmatic attack in 2008 at<br />
Banpong Hospital and had > 3 episodes <strong>of</strong> wheeze with good response to nebulized bronchodilators were randomized into<br />
two groups. Ketotifen group (n = 16) were given oral ketotifen 0.5 mg or 1 mg twice daily depending on age. Budesonide<br />
group (n = 14) were given as inhaled budesonide 200 μg (MDI) twice daily. Caregivers recorded children's asthmatic symptoms<br />
and nebulized treatments in diaries every day. The enrolled children received these two treatment regimens and were<br />
followed up for 26 weeks. Results: Number <strong>of</strong> ER visits decreased significantly after both treatments (p < 0.005). The<br />
percentage <strong>of</strong> children with reduction in ER visits was comparable between ketotifen and budesonide (p = 0.16). Ketotifen<br />
group also demonstrated a reduction in days <strong>of</strong> hospital stay (p < 0.05). Budesonide treatment resulted in more symptomfree<br />
days (p < 0.05). Both medications were well tolerated and safe. The only demonstrated side effect <strong>of</strong> ketotifen was<br />
weight gain. The growth rate in height for both groups did not differ. Conclusion: Both ketotifen and inhaled budesonide are<br />
effective, safe, and well-tolerated in the prevention <strong>of</strong> asthma exacerbation in children particularly in the country with limited<br />
resource.<br />
No.1469<br />
Author (s) : Tantilipikorn P., Thanaviratananich S., Chusakul S., Benjaponpitak S., Fooanant S., Chintrakarn C., Jirapongsananuruk O.,<br />
Visitsunthorn N., Toler T., Sutton L., Wu W., Lee L.<br />
Title : Efficacy and Safety <strong>of</strong> Once Daily Fluticasone Furoate Nasal Spray for Treatment <strong>of</strong> Irritant (Non-allergic) Rhinitis.<br />
Source : The Open Respiratory Medicine Journal, <strong>2010</strong>, 4, 92 – 99<br />
Document Type : Article<br />
Keywords : Fluticasone furoate, irritant, rhinitis<br />
<strong>Abstract</strong> : Introduction: The safety and efficacy <strong>of</strong> fluticasone furoate nasal spray (FFNS) for the symptoms <strong>of</strong> seasonal and perennial<br />
allergic rhinitis have been previously demonstrated in several clinical studies. The objective <strong>of</strong> this pilot study was to<br />
compare the efficacy and safety <strong>of</strong> FFNS 110 mcg once daily with placebo in patients with irritant (nonallergic) rhinitis<br />
triggered predominantly by air pollution. Methods: This was a randomized, double-blind, placebo-controlled, 4-week study <strong>of</strong><br />
subjects in Thailand with irritant rhinitis who received either FFNS 110 mcg (N=53) or placebo (N=49) once-daily. Subjects with<br />
a ≥2 year history <strong>of</strong> air pollution as their predominant rhinitis trigger, negative skin test to local seasonal/perennial allergens,<br />
positive histamine skin test, and normal sinus radiograph were enrolled if they met minimum reflective total nasal symptom<br />
score (rTNSS) ≥4.5 (maximum=9) and a nasal congestion score <strong>of</strong> ≥2 (maximum =3). All were assessed for nasal eosinophilia<br />
at study entry and completion. Air quality was monitored throughout the study. The primary efficacy endpoint was the mean<br />
change from baseline over the treatment period in daily rTNSS, the average <strong>of</strong> the morning and evening rTNSS. The key<br />
secondary measure was the mean change from baseline over the entire treatment period in morning pre-dose instantaneous<br />
total nasal symptom score (iTNSS). Results: The Air Quality Index (AQI) during the study generally did not reach unhealthy<br />
levels. Baseline daily rTNSS scores were similar between treatment groups (FFNS=6.7; placebo=6.4). The least square mean<br />
change from baseline in rTNSS was -2.17 and -2.10 for FFNS and placebo, respectively, with a difference <strong>of</strong> -0.065 (p=0.845).<br />
Gradual improvements were seen in both treatment groups for iTNSS; however, the treatment difference (-0.075) was not<br />
statistically significant (p=0.827). Additionally, there were no statistically significant differences between treatment groups for<br />
AM and PM rTNSS, individual nasal symptoms, daily reflective, AM and PM reflective and AM pre-dose instantaneous total<br />
ocular symptom scores (TOSS) or individual ocular symptom scores. Nasal cytology at baseline found more than two-thirds <strong>of</strong><br />
subjects had
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.1470<br />
Author (s) : Tantilipikorn, P., Vichyanond, P., Lacroix, J.S.<br />
Title : Nasal provocation test: How to maximize its clinical use?<br />
Source : Asian Pacific Journal <strong>of</strong> Allergy and Immunology, Volume 28, Issue 4, December <strong>2010</strong>, Pages 225-231<br />
Document Type : Review<br />
Keywords : Acoustic rhinometry; Allergic rhinitis; Diagnosis; Nasal provocation test; Peak nasal inspiratory flow; Rhinomanometry; Skin<br />
prick test<br />
<strong>Abstract</strong> : In order to diagnose allergic rhinitis (AR), skin prick tests and serum specific IgE level are the most common used methods.<br />
But there are some conditions which the results <strong>of</strong> both methods do not correlate with the clinical presentation <strong>of</strong> AR. Nasal<br />
provocation test is the method <strong>of</strong> detecting local IgE at the shock organ. There are some variations <strong>of</strong> NPT in terms <strong>of</strong> dosage,<br />
allergen administration, evaluation and scoring system. This article summarized the usefulness <strong>of</strong> NPT, its indication and<br />
contraindication, dosage and instillation techniques for allergens and evaluation <strong>of</strong> outcome in the hope that if we can<br />
standardize the procedure and make it easier to perform, NPT will be applied more in clinical practice. In addition normal<br />
values among Asian ethnics are presented for appropriate interpretation <strong>of</strong> the test.<br />
No.1471<br />
Author (s) : Tantipopipat, S., Boonpraderm, A., Charoenkiatkul, S., Wasantwisut, E., Winichagoon, P.<br />
Title : Dietary intake <strong>of</strong> spices and herbs in Habitual northeast thai diets<br />
Source : Malaysian Journal <strong>of</strong> Nutrition, Volume 16, Issue 1, <strong>2010</strong>, Pages 137-148<br />
Document Type : Article<br />
Keywords : Herbs; Northeast diets; Polyphenols; Portion size; Spices; Thailand<br />
<strong>Abstract</strong> : Spices and herbs are rich in polyphenols and widely used in habitual diets in the tropical regions. To elucidate their effects<br />
on human health, intake <strong>of</strong> the portion <strong>of</strong> spices and herbs from habitual diets should be determined. Consumption patterns<br />
were determined from 24-hour records or recalls <strong>of</strong> 181 men and 370 women in Khonkaen and Ubon Ratchathani provinces,<br />
representing upper and lower northeast Thailand. There was a slight variation in dishes, but twelve spices/herbs were<br />
commonly used in the two areas. The amounts <strong>of</strong> spices/ herbs in the four most common dishes (Somtum, Jaew, Pon and<br />
Kang-Nor-Mai) were estimated by weighing ingredients before and after cooking. The average amount <strong>of</strong> spices/herbs<br />
consumed was 4.9, 26.1, 14 and 11 g/meal, contributing 36.6, 43.1, 20.6 and 29.8 mg polyphenols/meal for Somtum, Jaew,<br />
Pon and Kang-Nor-Mai, respectively. Chili was common in all recipes, with an average amount <strong>of</strong> 8.3-27.5 mg<br />
polyphenols/meal. In conclusion, habitual northeast Thai diets contain several spices/herbs and a substantial amount <strong>of</strong><br />
polyphenols was commonly consumed.<br />
No.1472<br />
Author (s) : Tantirungrotechai, J.; Chotmongkolsap, P.; Pohmakotr, M.<br />
Title : Synthesis, characterization, and activity in transesterification <strong>of</strong> mesoporous Mg-Al mixed-metal oxides<br />
Source : MICROPOROUS AND MESOPOROUS MATERIALS, Volume: 128, Issue: 1-3, Pages: 41-47, DOI: 10.1016/j.micromeso.2009.08.001,<br />
Published: MAR <strong>2010</strong><br />
Document Type : Article<br />
Keywords : Mesoporous metal oxide; Mixed-metal oxide; Solid base; Transesterification; Biodiesel<br />
<strong>Abstract</strong> : In this work, a series <strong>of</strong> Mg-Al mixed-metal oxides with Mg/Al ratios <strong>of</strong> 0.125-8 were synthesized via a sol-gel method using<br />
cetyltrimethylammonium bromide as a template. All oxide samples were characterized by XRD, XRF, IR, DTA-TGA, TEM, and<br />
N(2) adsorption-desorption measurements. The phases <strong>of</strong> the Mg-Al mixed-metal oxides are the mixture <strong>of</strong> gamma-Al(2)O(3)<br />
and periclase (MgO) or the mixture <strong>of</strong> hydrotalcite (Mg(6)Al(2)CO(3)(OH)(16)center dot 4H(2)O) and periclase depending on the<br />
Mg/Al ratio. The Mg-Al oxides have mesoporous structure with surface areas in the range <strong>of</strong> 120-270 m(2)/g. These oxides<br />
were also impregnated with KI to increase their base strength, and their activities for biodiesel production were tested via the<br />
transesterification <strong>of</strong> soybean oil with methanol. The KI impregnated Mg-Al mixed-metal oxide at Mg/Al ratio <strong>of</strong> 4:1 has base<br />
strength in the range <strong>of</strong> 9.8
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Source : Tuberculosis, Volume 90, Issue 5, September <strong>2010</strong>, Pages 311-318<br />
Document Type : Article<br />
Keywords : Mycobacterium; Regulatory element; VNTR (variable number <strong>of</strong> tandem repeat)<br />
<strong>Abstract</strong> : VNTR (variable number <strong>of</strong> tandem repeats) has been used extensively for Mycobacterium tuberculosis strain discrimination. In<br />
contrast, their biological roles have been rarely investigated. We, herewith, studied whether two VNTR could promote<br />
expression <strong>of</strong> a downstream reporter gene, gfp. The VNTR loci, VNTR0960c and VNTR4052, reside upstream <strong>of</strong> the<br />
translational start sites <strong>of</strong> Rv0861c (helicase gene, ercc3) and Rv3610c (ftsH), respectively. Both are highly polymorphic among<br />
clinical strains <strong>of</strong> M. tuberculosis. DNA fragments containing various numbers <strong>of</strong> the repeat units were amplified and inserted<br />
upstream <strong>of</strong> the gfp gene and transformed into M. smegmatis mc2155 and M. tuberculosis H37Ra. The levels <strong>of</strong> fluorescence<br />
were determined by microplate fluorometry and flow cytometry. It was found that VNTR0960c could promote the expression<br />
<strong>of</strong> gfp while VNTR4052 alone could not. However, VNTR4052 was needed for complementing the promoter activity <strong>of</strong> the<br />
downstream sequence. The effects were discernible in M. tuberculosis, even though only the incomplete copy <strong>of</strong> the repeat<br />
was present. The addition <strong>of</strong> a complete copy <strong>of</strong> the repeat augmented the promoter activity. The presence <strong>of</strong> more copies<br />
<strong>of</strong> the repeat had minimal effects or even decreased the expressions. Significance <strong>of</strong> the effects on the cellular metabolisms<br />
<strong>of</strong> M. tuberculosis warrants further studies. © <strong>2010</strong> Elsevier Ltd. All rights reserved.<br />
No.1474<br />
Author (s) : Tantrakarnapa, K., Makkaew, P., Vatanasomboon, P., Kengganpanich, T.<br />
Title : Association <strong>of</strong> sanitary conditions and bacteriological quality <strong>of</strong> tube ice in ice plants in metropolitan Bangkok, Thailand<br />
Source : EnvironmentAsia, Volume 3, Issue 1, January <strong>2010</strong>, Pages 8-12<br />
Document Type : Article<br />
Keywords : Bacteriological quality; Ice plant; Ice tube; Sanitary conditions<br />
<strong>Abstract</strong> : This investigation aimed at studying the correlation between ice plant sanitary conditions and bacteriological quality <strong>of</strong> ice.<br />
The sanitary conditions in accordance with GMP regulations, the bacteriological quality <strong>of</strong> tube ice, and the processing water<br />
in 20 plants in Bangkok were studied. A modified inspection form from the Office <strong>of</strong> Food and Drug Administration was used<br />
for sanitary condition surveys. Ice and processing water samples were collected 2 times per plant with 3 samples each time.<br />
All 120 samples were analyzed for bacteriological quality by means <strong>of</strong> Standard Plate Count technique (Pour plate method)<br />
and Most Probable Number technique (MPN method). The results indicated that forty percent (8 Tube plants) failed and the<br />
remaining passed sanitary condition criteria. 23% <strong>of</strong> Tube Ice samples were acceptable in the standard permission level in<br />
terms <strong>of</strong> Total coliform bacteria and Fecal coliform bacteria. All Tube Ice plant processing water samples were acceptable in<br />
comparison with the standard permission level. Ice plant sanitary conditions were significantly correlated to bacteriological<br />
quality <strong>of</strong> the ice (p
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Despite almost identical IgE binding, rDer p 2 prepared from both E. coli and P. pastoris showed structural differences to nDer<br />
p 2. Der p 2 secreted from P. pastoris lacked most <strong>of</strong> the natural structure, but refolding could induce the natural structure.<br />
Copyright © 2009 S. Karger AG.<br />
No.1476<br />
Author (s) : Taphey, M., Pornkul, R.<br />
Title : Acute suppurative thyroiditis due to pyriform sinus fistula: A case report<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 3, March <strong>2010</strong>, Pages 388-392<br />
Document Type : Article<br />
Keywords : Acute suppurative thyroiditis; Pyriform sinus fistula<br />
<strong>Abstract</strong> : Congenital pyriform sinus fistula is an unusual developmental abnormality, but it is the most common cause <strong>of</strong> acute<br />
suppurative thyroiditis or recurrent lateral neck infection in pediatric group. It is <strong>of</strong>ten over-looked because <strong>of</strong> poor diagnostic<br />
roles. Further investigations are important for diagnosis and evaluation. The authors herein present a case <strong>of</strong> pyriform sinus<br />
fistula and diagnostic roles to confirm the diagnosis.<br />
No.1477<br />
Author (s) : Tatanun, C., Ritthipravat, P., Bhongmakapat, T., Tuntiyatorn, L.<br />
Title : Automatic segmentation <strong>of</strong> nasopharyngeal carcinoma from CT images: Region growing based technique<br />
Source : ICSPS <strong>2010</strong> - Proceedings <strong>of</strong> the <strong>2010</strong> 2nd <strong>International</strong> Conference on Signal Processing Systems, Volume 2, <strong>2010</strong>, Article<br />
number 5555663, Pages V2537-V2541<br />
Document Type : Conference Paper<br />
Keywords : Automatic segmentation; Nasopharyngeal carcinoma; Region growing technique<br />
<strong>Abstract</strong> : This paper describes a framework for automatic nasopharyngeal carcinoma segmentation from CT images. The proposed<br />
technique is based on the Region Growing Method. It is automatic segmentation in which an initial seed is generated without<br />
human intervention. The seed is generated from a probabilistic map representing the chances <strong>of</strong> it being tumor. This map is<br />
created from three probabilistic functions based on location <strong>of</strong> the tumor, intensities, and non-tumor region respectively. The<br />
pixel in which the probability is the highest will be selected as potential seeds. Only one representative <strong>of</strong> these seeds will<br />
be selected as an initial seed. Then the seed will be used for region growing subsequently. The experimental results showed<br />
that the potential seeds and initial seed were correctly determined with a percentage accuracy <strong>of</strong> 81.60% and 95.10%. The<br />
seed was grown in preprocessed CT images for identifying the nasopharyngeal carcinoma region. The results showed that,<br />
perfect match and corresponding ratio were 71.31% and 53.00% respectively © <strong>2010</strong> IEEE.<br />
No.1478<br />
Author (s) : Taveecharoenkool, T., Angsuthanasombat, C., Kanchanawarin, C.<br />
Title : Combined molecular dynamics and continuum solvent studies <strong>of</strong> the pre-pore Cry4Aa trimer suggest its stability in solution<br />
and how it may form pore<br />
Source : PMC Biophysics, Volume 3, Issue 1, 13 May <strong>2010</strong>, Article number 10<br />
Document Type : Article<br />
Keywords : –<br />
<strong>Abstract</strong> : Cry4Aa toxin is one <strong>of</strong> the highly specific mosquito-larvicidal proteins produced by the bacterium Bacillus thuringiensis<br />
subspecies israelensis. It is thought to form pores in the larval midgut membrane that cause membrane leakage and<br />
subsequent insect death. Therefore, Cry4Aa and other Cry toxins have been used as efficient and safe bacterial insecticides to<br />
control the disease-carrying mosquitoes such as Aedes Anopheles, and Culex. However, we still do not clearly understand<br />
how Cry toxins kill mosquito-larvae at molecular details. Recent electron crystallographic images <strong>of</strong> Cry4Ba toxin, another<br />
toxin closely related to Cry4Aa toxin, have suggested that the protein forms trimer in aqueous solution and in lipid<br />
monolayer. Moreover, the unit cell <strong>of</strong> X-ray crystal structure <strong>of</strong> Cry4Ba toxin has been shown to be trimeric. In this study, we<br />
constructed the first full-atom structural model <strong>of</strong> Cry4Aa trimer using the trimeric unit cell structure <strong>of</strong> Cry4Ba toxin as a<br />
template and then used the methods <strong>of</strong> molecular dynamics (MD) and molecular mechanics combined with Poisson-<br />
Boltzmann and surface area (MM-PBSA) to show that the trimeric structure <strong>of</strong> Cry4Aa toxin is stable in 150 mM KCl solution<br />
on 10 ns timescale. The results reveal that Cry4Aa toxins use polar amino acid residues on α-helices 3, 4, and 6 to form<br />
trimer and suggest that the proteins form trimer to reduce their non-polar interactions with surrounding water. Based on the<br />
obtained trimeric structure <strong>of</strong> Cry4Aa toxins, we propose that pore formation <strong>of</strong> Cry toxins may involve a 90°-hairpin rotation<br />
537
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
during the insertion <strong>of</strong> their three α4-α5 hairpins into the membrane. This process may be mediated by water and ions. ©<br />
<strong>2010</strong> Taveecharoenkool et al.<br />
No.1479<br />
Author (s) : Taylor, W.R.J.; Burhan, E.; Wertheim, H.; Soepandi, P.Z.; Horby, P.; Fox, A.; Benamore, R.; de Simone, L.; Hien, T.T.; Chappuis, F.<br />
Title : Avian influenza - A review for doctors in travel medicine<br />
Source : TRAVEL MEDICINE AND INFECTIOUS DISEASE, Volume: 8, Issue: 1, Pages: 1-12, DOI: 10.1016/j.tmaid.2009.11.006, Published: JAN<br />
<strong>2010</strong><br />
Document Type : Article<br />
Keywords : Avian influenza; Travellers; H5N1; ARDS<br />
<strong>Abstract</strong> : First identified in humans in Hong Kong, influenza A/H5N1, known commonly as avian influenza, has caused human disease in<br />
15 countries around the world. Although the current number <strong>of</strong> confirmed patients is tiny compared to seasonal and the<br />
recently emerged H1N1 'swine' influenza, H5N1 remains a candidate for the next highly pathogenic influenza pandemic.<br />
Currently, H5N1 has very limited ability to spread from person-to-person but this may change because <strong>of</strong> mutation or<br />
reassortment with other influenza viruses leading to an influenza pandemic with high mortality. If this occurs travellers are<br />
likely to be affected and travel medicine doctors will need to consider avian influenza in returning febrile travellers. The early<br />
clinical features may be dismissed easily as 'the flu' resulting in delayed treatment. Treatment options are limited. Oral<br />
oseltamivir alone has been the most commonly used drug but mortality remains substantial, up to 80% in Indonesia.<br />
Intravenous peramivir has been filed for registration and IV zanamivir is being developed. This review will focus on the<br />
epidemiological and clinical features <strong>of</strong> influenza A/H5N1 avian influenza and will highlight aspects relevant to travel<br />
medicine doctors. (C) 2009 Elsevier Ltd. All rights reserved.<br />
No.1480<br />
Author (s) : Taytawat, P., Viravud, Y., Plakornkul, V., Roongruangchai, J., Manoonpol, C.<br />
Title : Identification <strong>of</strong> the external laryngeal nerve: Its anatomical relations to inferior constrictor muscle, superior thyroid artery,<br />
and superior pole <strong>of</strong> the thyroid gland in Thais<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 8, August <strong>2010</strong>, Pages 961-968<br />
Document Type : Article<br />
Keywords : External laryngeal nerve; Inferior constrictor muscle; Superior thyroid artery; Thyroid surgery<br />
<strong>Abstract</strong> : Background: The external laryngeal nerve (ELN) is not routinely identified during thyroid surgery despite clear benefits from<br />
doing so. One reason is its anatomical variations, which differ from papers to papers. There were also very few studies <strong>of</strong> ELN<br />
in Thais, especially its relation to inferior constrictor muscle and tip <strong>of</strong> superior pole <strong>of</strong> the thyroid gland, which serve as<br />
important landmarks when identifying the ELN intra operatively. Objective: To describe the course <strong>of</strong> ELN and its relations to<br />
the inferior constrictor muscle, superior thyroid artery (STA), and tip <strong>of</strong> superior pole <strong>of</strong> the thyroid gland in Thais in order to<br />
serve as a guide for identification and preservation <strong>of</strong> the nerve. Material and Method: One hundred thirty four ELNs from 68<br />
cadavers with normal-sized thyroid glands and no signs <strong>of</strong> neck abnormality were studied. Three areas were studied, (1) its<br />
relation to inferior constrictor muscle, (2) the nearest distance from the nerve to tip <strong>of</strong> the superior pole <strong>of</strong> the thyroid gland,<br />
and (3) the crossing point <strong>of</strong> the ELN and STA in relation to the tip <strong>of</strong> the superior pole <strong>of</strong> the thyroid gland. All distances<br />
were measured in mm using avernier caliper. Results: The three areas <strong>of</strong> the study were reported separately using established<br />
classifications and compared to the previous published papers. All ELNs run superficially, at the least on the upper portion <strong>of</strong><br />
the inferior constrict muscle, rendering it more vulnerable to injury in Thais. Conclusion: The course <strong>of</strong> the ELN and its<br />
relations to nearby structures can vary considerably and are influenced by many factors. Nevertheless, these findings should<br />
encourage the surgeons to identify the ELN intraoperatively with meticulous dissection to minimize the risk <strong>of</strong> ELN injury.<br />
No.1481<br />
Author (s) : Teamsuwan, Y., Kaeoket, K., Tienthai, P., Tummaruk, P.<br />
Title : Morphological changes and infiltration <strong>of</strong> immune cells in the endometrium <strong>of</strong> anoestrus gilt in relation to the ovarian<br />
appearance and serum progesterone<br />
Source : Thai Journal <strong>of</strong> Veterinary Medicine, Volume 40, Issue 1, March <strong>2010</strong>, Pages 31-40<br />
Document Type : Article<br />
Keywords : Anoestrus; Endometrium; Gilt; Immune cell; Progesterone<br />
538
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : The present study investigates morphological changes and distribution <strong>of</strong> the leukocyte subpopulation in the endometrium <strong>of</strong><br />
anoestrus gilts in relation to reproductive cycles and serum progesterone (P4). Selected genital organs from 30 gilts culled<br />
due to anoestrus were examined. The genital organs were classified according to the ovarian appearance into 3 groups, i.e.<br />
inactive (n = 10); follicular (n = 10); and luteal phase (n = 10). Blood samples were collected prior to slaughter to determine<br />
serum P4. Seven tissue samples were randomly collected from the uteri <strong>of</strong> the gilts and were examined for histological<br />
structures, i.e. epithelial types and height, number <strong>of</strong> blood vessel, secretory vesicle and endometrial glands. Number <strong>of</strong><br />
leukocyte subsets, i.e. lymphocytes, neutrophils, eosinophils, macrophages and plasma cells were counted. On average, age<br />
and body weight at culling <strong>of</strong> the gilts were 306.4±39.9 d (range 233-407 d) and 150.4±24.8 kg (range 104.0-205.5 kg).<br />
Lymphocyte was the most common immune cell in all tissue layers and in all stages <strong>of</strong> the reproductive cycle. Lymphocytes<br />
in glandular layer in the inactive phase was higher than in the follicular (p=0.02) and luteal phases (p=0.05). Neutrophils in<br />
both epithelial and subepithelial layers in follicular phases was higher than luteal and inactive phases (p<0.001). Eosinophil<br />
in subepithelium in the luteal phase was higher than inactive (p=0.004) and follicular phases (p<0.001). An increase in the<br />
serum P4 resulted in an increase number <strong>of</strong> uterine glands (p<0.001), a decrease number <strong>of</strong> lymphocytes in all tissue layers<br />
(p<0.05), a decrease number <strong>of</strong> neutrophils in subepithelial layers (p=0.03) and an increase in the number <strong>of</strong> eosinophils in<br />
subepithelial layers (p<0.001). In conclusion, the infiltration <strong>of</strong> the leukocyte subpopulation in the endometrium <strong>of</strong><br />
anoestrus gilts is largely dependent on the ovarian function. Neutrophils and eosinophils were common immune cells in<br />
follicular and luteal phases, respectively.<br />
No.1482<br />
Author (s) : Techaprasan, J., Klinbunga, S., Ngamriabsakul, C., Jenjittikul, T.<br />
Title : Genetic variation <strong>of</strong> kaempferia (zingiberaceae) in thailand based on chloroplast dna (psba-trnh and peta-psbj) sequences<br />
Source : Genetics and Molecular Research, Volume 9, Issue 4, <strong>2010</strong>, Pages 1957-1973<br />
Document Type : Article<br />
Keywords : Chloroplast dna; Dna barcode; Kaempferia; Peta-psbj; Psba-trnh<br />
<strong>Abstract</strong> : Genetic variation and species authentication <strong>of</strong> 71 Kaempferia accessions (representing 15 recognized, six new, and four<br />
unidentified species) found indigenously in Thailand were examined by determining chloroplast psbA-trnH and partial petApsbJ<br />
spacer sequences. Ten closely related species (Boesenbergia rotunda, Gagnepainia godefroyi, G. thoreliana, Globba<br />
substrigosa, Smithatris myanmarensis, S. supraneanae, Scaphochlamys biloba, S. minutiflora, S. rubescens, and Stahlianthus<br />
sp) were also included. After sequence alignments, 1010 and 865 bp in length were obtained for the respective chloroplast<br />
DNA sequences. Intraspecific sequence variation was not observed in Kaempferia candida, K. angustifolia, K. laotica, K. galanga,<br />
K. pardi sp nov., K. bambusetorum sp nov., K. albomaculata sp nov., K. minuta sp nov., Kaempferia sp nov. 1, and G.<br />
thoreliana, for which more than one specimen was available. In contrast, intraspecific sequence polymorphisms were<br />
observed in various populations <strong>of</strong> K. fallax, K. filifolia, K. elegans, K. pulchra, K. rotunda, K. marginata, K. parviflora, K. larsenii,<br />
K. roscoeana, K. siamensis, and G. godefroyi A strict consensus tree based on combined psbA-trnH and partial petApsbJ<br />
sequences revealed four major groups <strong>of</strong> Kaempferia species. We suggest that the genus Kaempferia is a polyphyletic group,<br />
as K. candida was distantly related and did not group with other Kaempferia species. Polymorphic sites and indels <strong>of</strong> psbAtrnH<br />
and petA-psbJ can be used as DNA barcodes for species diagnosis <strong>of</strong> most Kaempferia and outgroup species. Nuclear<br />
DNA polymorphism should be examined to determine if there has been interspecific hybridization and chloroplast DNA<br />
introgression in these taxa. © FUNPEC-RP.<br />
No.1483<br />
Author (s) : Techasaensiri, C.; Messina, A.F.; Katz, K.; Ahmad, N.; Huang, R.; McCracken, G.H., Jr.<br />
Title : Epidemiology and Evolution <strong>of</strong> Invasive Pneumococcal Disease Caused by Multidrug Resistant Serotypes <strong>of</strong> 19A in the 8 Years<br />
After Implementation <strong>of</strong> Pneumococcal Conjugate Vaccine Immunization in Dallas, Texas<br />
Source : PEDIATRIC INFECTIOUS DISEASE JOURNAL, Volume: 29, Issue: 4, Pages: 294-300, DOI: 10.1097/INF.0b013e3181c2a229,<br />
Published: APR <strong>2010</strong><br />
Document Type : Article<br />
Keywords : pneumococcus; serotype; invasive pneumococcal disease; 10-year experience after pneumococcal vaccine<br />
<strong>Abstract</strong> : Background: The heptavalent pneumococcal conjugate vaccine (PCV7) has significantly reduced vaccine-type invasive<br />
pneumococcal disease (IPD) in children. An increasing percentage <strong>of</strong> IPD cases are now caused by nonvaccine serotypes. The<br />
purpose <strong>of</strong> our observational study was to define the epidemiology <strong>of</strong> pneumococcal disease in Dallas, TX children for 8<br />
years after implementation <strong>of</strong> PCV7 immunization. Methods: Streptococcus pneumoniae isolates from normally sterile sites<br />
539
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
were collected at Children's Medical Center <strong>of</strong> Dallas from January 1, 1999 to December 31, 2008. Incidence <strong>of</strong> IPD was<br />
calculated using inpatient and emergency center admissions to Children's Medical Center <strong>of</strong> Dallas as the denominator.<br />
Isolates were serotyped and penicillin and cefotaxime susceptibilities were determined. Serotype 19A isolates were further<br />
characterized by multilocus sequence typing. Results: Compared with the prevaccine period <strong>of</strong> 1999-2000, there was a<br />
significant reduction in the incidence <strong>of</strong> IPD from 2002 to 2008 ( P < 0.05), although a significant increase in IPD incidence was<br />
observed from 2006 to 2008 (P = 0.038). The number <strong>of</strong> IPD cases caused by serotype 19A increased from 1999 to 2008 (P <<br />
0.001). There were significant increases in penicillin and cefotaxime nonsusceptibile 19A isolates during this 10-year period (P<br />
< 0.001 and P = 0.004, respectively). The most common sequence type (ST) <strong>of</strong> the 19A isolates was ST-199 (42.7%). Clonal<br />
complex (cc-156) and cc-320 emerged in the period <strong>of</strong> 2005-2008 as penicillin and cefotaxime resistant 19A strains.<br />
Conclusions: In Dallas, PCV7 immunization reduced significantly the incidence <strong>of</strong> IPD caused by vaccine-type strains. A<br />
significant increase in IPD caused by serotype 19A was observed. The penicillin and cefotaxime nonsusceptible STs, not<br />
previously identified in Dallas, have recently become an important cause <strong>of</strong> IPD.<br />
No.1484<br />
Author (s) : Teerasong, S., Amornthammarong, N., Grudpan, K., Teshima, N., Sakai, T., Nacapricha, D., Ratanawimarnwong, N.<br />
Title : A Multiple processing hybrid flow system for analysis <strong>of</strong> formaldehyde contamination in food<br />
Source : Analytical Sciences, Volume 26, Issue 5, May <strong>2010</strong>, Pages 629-633<br />
Document Type : Article<br />
Keywords : formaldehyde; Agaricales, animal, article, calibration, cephalopod, chemistry, flow injection analysis, food<br />
contamination, instrumentation, limit <strong>of</strong> detection, methodology, reproducibility, standard, time, vegetable;<br />
Agaricales, Animals, Calibration, Decapodiformes, Flow Injection Analysis, Food Contamination, Formaldehyde, Limit <strong>of</strong><br />
Detection, Reference Standards, Reproducibility <strong>of</strong> Results, Time Factors, Vegetables<br />
<strong>Abstract</strong> : This work proposes a flow system suitable for the rapid screening <strong>of</strong> formaldehyde contaminated in food. The system is<br />
based on the concept <strong>of</strong> a flow analyzer with a Hantzsch reaction. An operating procedure was developed for multiple<br />
tasking and high sample throughput. This resulted in a significant sample throughput <strong>of</strong> 51 samples h-1. Under the optimized<br />
conditions, linear calibration from 10 to 100 μM was obtained. The system gave a limit <strong>of</strong> detection and a limit <strong>of</strong><br />
quantitation <strong>of</strong> 0.06 and 0.10 mg kg-1, respectively. The system was successfully applied to re-hydrated dry squids,<br />
vegetables and mushrooms. <strong>2010</strong> © The Japan Society for Analytical Chemistry.<br />
No.1485<br />
Author (s) : Teerasong, S., Chan-Eam, S., Sereenonchai, K., Amornthammarong, N., Ratanawimarnwong, N., Nacapricha, D.<br />
Title : A reagent-free SIA module for monitoring <strong>of</strong> sugar, color and dissolved CO2 content in s<strong>of</strong>t drinks<br />
Title : Analytica Chimica Acta, Volume 668, Issue 1, May <strong>2010</strong>, Pages 47-53<br />
Document Type : Article<br />
Keywords : Dissolved CO2; Membraneless; Schlieren effect; Sequential injection analysis; S<strong>of</strong>t drink; Sucrose content<br />
<strong>Abstract</strong> : This work presents a new sequential injection analysis (SIA) method and a module for simultaneous and real-time monitoring<br />
<strong>of</strong> three key parameters for the beverage industry, i.e., the sugar content (measured in Brix), color and dissolved CO2.<br />
Detection <strong>of</strong> the light reflection at the liquid interface (the schlieren effect) <strong>of</strong> sucrose and water was utilized for sucrose<br />
content measurement. A near infrared LED (890±40nm) was chosen as the light source to ensure that all the ingredients and<br />
dyes in s<strong>of</strong>t drinks will not interfere by contributing light absorption. A linear calibration was obtained for sucrose over a wide<br />
concentration range (3.1-46.5Brix). The same module can be used to monitor the color <strong>of</strong> the s<strong>of</strong>t drink as well as the<br />
dissolved CO2 during production. For measuring the color, the sample is segmented between air plugs to avoid dispersion. An<br />
RGB-LED was chosen as the light source in order to make this module applicable to a wide range <strong>of</strong> colored samples. The<br />
module also has a section where dissolved CO2 is measured via vaporization <strong>of</strong> the gas from the liquid phase. Dissolved CO2,<br />
in a flowing acceptor stream <strong>of</strong> water resulting in the change <strong>of</strong> the acceptor conductivity, is detected using an in-house<br />
capacitively coupled contactless conductivity detector (C4D). The module includes a vaporization unit that is also used to<br />
degas the carbonated drink, prior the measurements <strong>of</strong> sucrose and color within the same system. The method requires no<br />
chemicals and is therefore completely friendly to the environment. © <strong>2010</strong> Elsevier B.V.<br />
No.1486<br />
Author (s) : Teerasong, S., Chantore, W., Ruenwongsa, P., Nacapricha, D.<br />
Title : Development <strong>of</strong> a predict-observe-explain strategy for teaching flow injection at undergraduate chemistry<br />
540
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Source : <strong>International</strong> Journal <strong>of</strong> Learning, Volume 17, Issue 8, <strong>2010</strong>, Pages 137-150<br />
Document Type : Article<br />
Keywords : Demonstration; Flow injection; Predict-observe-explain; Undergraduate students<br />
<strong>Abstract</strong> : Although traditional lecture is <strong>of</strong>ten regarded as a passive way <strong>of</strong> learning, most <strong>of</strong> the teachings in university including<br />
science subjects are indeed carried out by this method. Teaching <strong>of</strong> flow injection and flow phenomena to undergraduate<br />
students in chemistry can be boring if traditional lecture is the only method the teacher uses. In this work, a demonstration<br />
together with 'predict-observeexplain' (POE) teaching approach was developed. The demonstration equipment set, assembled<br />
using transparent materials for clear observation <strong>of</strong> the flow phenomena, together with POE teaching strategy was introduced<br />
to the students. The POE approach <strong>of</strong>fers real-time probing <strong>of</strong> student understanding. Students were first asked to predict,<br />
observe the demonstration, and finally to discuss the results with peers. This strategy provided an opportunity for students to<br />
generate their own conceptual knowledge via reconciliation and negotiation between their prior knowledge and new<br />
experience. The results revealed that the new phenomena. The questionnaire results showed that the students had positive<br />
attitude toward the new teaching strategy. The developed activity was well-fitted in a time period <strong>of</strong> classroom lecture. ©<br />
Common Ground, Saowapak Teerasong, Wanrawee Chantore, Pintip Ruenwongsa, Duangjai Nacapricha.<br />
No.1487<br />
Author (s) : Tewthanom, K., Janwitayanujit, S., Totemchockcyakarn, K., Panomvana Na Ayudhya, D.<br />
Title : The effect <strong>of</strong> high dose <strong>of</strong> N-acetylcysteine in lupus nephritis: A case report and literature review<br />
Source : Journal <strong>of</strong> Clinical Pharmacy and Therapeutics, Volume 35, Issue 4, August <strong>2010</strong>, Pages 483-485<br />
Document Type : Article<br />
Keywords : case report; lupus nephritis; N-acetylcysteine<br />
<strong>Abstract</strong> : An imbalance <strong>of</strong> oxidative-antioxidant defence mechanism has been proposed in systemic lupus erythematosus patients. Coadministration<br />
<strong>of</strong> N-acetylcysteine (NAC) which has a strong antioxidant activity may produce a satisfactory therapeutic<br />
outcome when added to standard therapy. We report a case <strong>of</strong> a 46-year-old lupus nephritis patient who received 1800 mg<br />
<strong>of</strong> NAC orally. After NAC, this patient showed a higher glutathione level, and a normal level <strong>of</strong> malondialdehyde, a lipid<br />
peroxidation product. In addition, the urinary protein levels, the complete blood counts and physical examination <strong>of</strong> the<br />
affected organs showed improvement. However, a well-controlled trial is needed to confirm the value <strong>of</strong> high-dose NAC in<br />
lupus nephritis patients. © <strong>2010</strong> Blackwell Publishing Ltd.<br />
No.1488<br />
Author (s) : Thaenkham, U., Dekumyoy, P., Komalamisra, C., Sato, M., Dung, D.T., Waikagul, J.<br />
Title : Systematics <strong>of</strong> the subfamily Haplorchiinae (Trematoda: Heterphyidae), based on nuclear ribosomal DNA genes and ITS2<br />
region<br />
Source : Parasitology <strong>International</strong>, Volume 59, Issue 3, September <strong>2010</strong>, Pages 460-465<br />
Document Type : Article<br />
Keywords : Internal transcribed spacer subunit II; Nuclear ribosomal DNA genes; Subfamily Haplorchiinae; Systematics<br />
<strong>Abstract</strong> : Phylogenetic relationships <strong>of</strong> 6 species in the trematode subfamily Haplorchiinae were analyzed using small and large subunit<br />
<strong>of</strong> ribosomal DNA genes (18S rDNA and 28S rDNA) and internal transcribed spacer subunit II (ITS2) region as molecular markers.<br />
Maximum Likelihood and Bayesian inference analyses <strong>of</strong> combined rDNAs and ITS2 indicated a close relationship between the<br />
genera Haplorchis and Procerovum, while these two genera were distinct from Stellantchasmus falcatus. These phylogenetic<br />
relationships were consistent with the number <strong>of</strong> testes but not with the characters <strong>of</strong> the modification <strong>of</strong> the seminal vesicle<br />
or <strong>of</strong> the ventral sucker. Although three Haplorchis spp. were, together with Procerovum, in the same cluster, their mutual<br />
topology was incongruent between rDNA and ITS2 trees. Phylogenetic analyses using other molecular markers with more<br />
species are necessary to work out solid phylogenetic relationships among the species in this subfamily. © <strong>2010</strong> Elsevier<br />
Ireland Ltd.<br />
No.1489<br />
Author (s) : Thaenkham, U., Nawa, Y.<br />
Title : Double strand problems: Reverse DNA sequences deposited in the DNA database<br />
Source : Korean Journal <strong>of</strong> Parasitology, Volume 48, Issue 1, March <strong>2010</strong>, Pages 89-90<br />
Document Type : Letter<br />
541
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : cyclooxygenase 1; Clonorchis sinensis, DNA sequence, gene sequence, genetic database, Gymnophalloides seoi, Haplorchis<br />
taichui, letter, Metagonimus miyatai, metagonimus takahashii, metagonimus yokogawai, Neodiplostomum<br />
seoulense, nonhuman, nucleotide sequence, Opisthorchis viverrini, Paragonimus bangkokensis, polymerase chain<br />
reaction, trematode; Animals, Base Sequence, Databases, Nucleic Acid, DNA, Helminth, DNA, Mitochondrial, Electron Transport<br />
Complex IV, Helminth Proteins<br />
<strong>Abstract</strong> : –<br />
No.1490<br />
Author (s) : Thaenkham, U., Nuamtanong, S., Sa-nguankiat, S., Yoonuan, T., Touch, S., Manivong, K., Vonghachack, Y., Sato, M., Waikagul, J.<br />
Title : Monophyly <strong>of</strong> Opisthorchis viverrini populations in the lower Mekong Basin, using mitochondrial DNA nad1 gene as the marker<br />
Source : Parasitology <strong>International</strong>, Volume 59, Issue 2, June <strong>2010</strong>, Pages 242-247<br />
Document Type : Article<br />
Keywords : nad1 gene; O. viverrini; Population structure<br />
<strong>Abstract</strong> : The liver fluke, Opisthorchis viverrini, causes serious public-health problems in the Lower Mekong Basin. This study aimed to<br />
clarify whether O. viverrini populations may be genetically divided into sub-specific taxa. We collected 6 populations <strong>of</strong> O.<br />
viverrini from different places in Cambodia, Lao PDR, and Thailand, along both sides <strong>of</strong> the Mekong River, and analyzed the<br />
population structure <strong>of</strong> these using the mitochondrial nad1 gene as a marker. The results <strong>of</strong> the DNA polymorphism<br />
measurements, by theta-w (θw) and -π (θπ) values, neutrality tests, and mismatch distribution, suggested that the<br />
population <strong>of</strong> O. viverrini has expanded under the influence <strong>of</strong> purifying selection and selective sweep. The analysis <strong>of</strong><br />
molecular variance (AMOVA) test revealed no significant genetic differences among the O. viverrini populations on opposite<br />
sides <strong>of</strong> the Mekong River. O. viverrini haplotypes occurred in multiple populations, and no distinct geographical clade. The<br />
star-like haplotype network confirmed a demographic expansion <strong>of</strong> the O. viverrini population. Overall, the genetic data from<br />
these populations suggested that the postulated existence <strong>of</strong> an O. viverrini species complex should be rejected. The biogeographical<br />
diversity <strong>of</strong> O. viverrini populations should be explored further, using other appropriate markers and a wider<br />
range <strong>of</strong> samples from geographically different areas. © <strong>2010</strong> Elsevier Ireland Ltd. All rights reserved.<br />
No.1491<br />
Author (s) : Thaipisuttikul, I; Adams, K.N.; Miller, S., I; Ernst, R.K.<br />
Title : ANAEROBIC GROWTH INDUCES A CYSTIC FIBROSIS SPECIFIC LIPID A MODIFICATION IN PSEUDOMONAS AERUGINOSA<br />
Source : PEDIATRIC PULMONOLOGY, Supplement: 33, Pages: 321-322, Published: <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1492<br />
Author (s) : Thamlikitkul, V., Tiengrim, S., Chalermsri, C., Chinsawangwatanakul, P., Suddhichupaiboon, S.<br />
Title : Microbiological equivalence <strong>of</strong> serum bacteriostatic and bactericidal activities <strong>of</strong> the sera from healthy volunteers receiving<br />
original meropenem (Meronem) and generic meropenem (Mero).<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand = Chotmaihet thangphaet, Volume 93 Suppl 1, January <strong>2010</strong>, Pages S110-116<br />
Document Type : Article<br />
Keywords : antiinfective agent, generic drug, meropenem, thienamycin derivative; adolescent, adult, article, bactericidal<br />
activity, bacterium, blood, clinical trial, controlled clinical trial, controlled study, crossover procedure, drug<br />
administration, drug effect, female, human, intravenous drug administration, male, methodology, middle<br />
aged, pharmacokinetics, randomized controlled trial, Thailand; Adolescent, Adult, Anti-Bacterial Agents, Bacteria, Cross-Over<br />
Studies, Drug Administration Schedule, Drugs, Generic, Female, Humans, Infusions, Intravenous, Male, Middle Aged, Serum<br />
Bactericidal Test, Thailand, Therapeutic Equivalency, Thienamycins, Young Adult<br />
<strong>Abstract</strong> : BACKGROUND: Several generic meropenem formulations have been approved by Thai Food and Drug Administration, Ministry<br />
<strong>of</strong> Public Health since 2008. Meropenem is a parenteral drug. Therefore, a study demonstrating a biological equivalence <strong>of</strong><br />
generic meropenem is not required for drug registration in Thailand. The objective <strong>of</strong> the study was to determine<br />
microbiological equivalence <strong>of</strong> serum bacteriostatic and bactericidal activities <strong>of</strong> the sera from healthy volunteers receiving<br />
original meropenem (Meronem) and generic meropenem (Mero). MATERIAL AND METHOD: This was a randomized crossover<br />
study in 16 adult healthy volunteers. Each subject received one gram <strong>of</strong> Meronem and Mero in 50 ml <strong>of</strong> normal saline via<br />
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intravenous infusion for 30 minutes. The blood samples were drawn at baseline prior to receiving the study drug, at 30<br />
minutes after initiating infusion, and at 8 hours after initiating infusion. The serum bacteriostatic activity against E. coli ATCC<br />
25922, K. pneumoniae, P. aeruginosa ATCC 27853 and A. baumannii was performed by disk diffusion. The serum bactericidal<br />
activity against E. coli ATCC 25922 was performed by Serum Bactericidal Titre. RESULTS: The average inhibition zone diameter<br />
<strong>of</strong> the serum samples from the subjects while receiving Mero against each tested organisms was < 1 mm smaller than that<br />
while receiving Meronem and such difference was not significantly different. All serum samples taken at 30 minutes after<br />
initiating Meronem and Mero had bactericidal titres against E. coli ATCC 25922 > or = 1:256. Only 3 serum samples taken from<br />
the subjects while receiving Mero at 8 hours had less bactericidal titre for 1-fold dilution when compared with that <strong>of</strong><br />
Meronem. CONCLUSION: The sera from healthy volunteers receiving Meronem and Mero had microbiological equivalence in<br />
terms <strong>of</strong> serum bacteriostatic and bactericidal activities.<br />
No.1493<br />
Author (s) : Thamlikitkul, V., Trakulsomboon, S.<br />
Title : In vitro activity <strong>of</strong> biapenem against Burkholderia pseudomallei<br />
Source : <strong>International</strong> Journal <strong>of</strong> Antimicrobial Agents, Volume 35, Issue 5, May <strong>2010</strong>, Page 514<br />
Document Type : Letter<br />
Keywords : biapenem, ceftazidime, doripenem, imipenem, meropenem; antibacterial activity, antibiotic sensitivity, bacterial<br />
strain, bacterium isolate, Burkholderia pseudomallei, controlled study, Escherichia coli, letter, minimum inhibitory<br />
concentration, nonhuman, priority journal, Pseudomonas aeruginosa, quality control; Anti-Bacterial Agents, Burkholderia<br />
pseudomallei, Humans, Melioidosis, Microbial Sensitivity Tests, Thienamycins<br />
<strong>Abstract</strong> : –<br />
No.1494<br />
Author (s) : Thammachat, S., Pungtanom, N., Kidsanguan, S., Pathaichindachote, W., Promdonkoy, B., Krittanai, C.<br />
Title : Amino acid substitution on β1 and αF <strong>of</strong> Cyt2Aa2 affects molecular interaction <strong>of</strong> protoxin<br />
Source : BMB Reports, Volume 43, Issue 6, <strong>2010</strong>, Pages 427-431<br />
Document Type : Article<br />
Keywords : Bacillus thuringiensis; Cytolytic toxin; Mutagenesis; Protein folding; Toxicity<br />
<strong>Abstract</strong> : Cyt2Aa2 is a mosquito-larvicidal protein produced as a 29 kDa crystalline protoxin from Bacillus thuringiensis subsp.<br />
darmstadiensis. To become an active toxin, proteolytic processing is required to remove amino acids from its N- and Ctermini.<br />
This study aims to investigate the functional role <strong>of</strong> amino acid residues on the N-terminal β1 and C-terminal αF <strong>of</strong><br />
Cyt2Aa2 protoxin. Mutant protoxins were constructed, characterized and compared to the wild type Cyt2Aa2. Protein<br />
expression data and SDS-PAGE analysis revealed that substitution at leucine-33 (L33) <strong>of</strong> β1 has a critical effect on dimer<br />
formation and structural stability against proteases. In addition, amino acids N230 and I233-F237 around the C-terminus αF<br />
demonstrated a crucial role in protecting the protoxin from proteolytic digestion. These results suggested that β1 and αF on<br />
the N- and C-terminal ends <strong>of</strong> Cyt2Aa2 protoxin play an important role in the molecular interaction and in maintaining the<br />
structural stability <strong>of</strong> the protoxin.<br />
No.1495<br />
Author (s) : Thammawong, C., Petchsuk, A., Opaprakasit, M., Chanunpanich, N., Tangboriboonrat, P., Opaprakasit, P.<br />
Title : Preparation and characterizations <strong>of</strong> electrospun lactide-based polymeric nan<strong>of</strong>ibers<br />
Source : Advanced Materials Research, Volume 93-94, <strong>2010</strong>, Pages 377-380<br />
Document Type : Conference Paper<br />
Keywords : Copolymer; Electrospinning; Nan<strong>of</strong>iber; Polylactic acid; Porosity<br />
<strong>Abstract</strong> : Poly(L)lactide (PLLA), aliphatic polyester, and poly(LLA-co-DLLA) copolymers consisting <strong>of</strong> 2.5, 7.5, 50% <strong>of</strong> DLLA content were<br />
also synthesized. PLLA was successfully electrospun by using 15wt% solution in (1DMF:3CHCl 3) mixed solvent. 2.5, 7.5, 50%<br />
P(LLA-co-DLLA) copolymers were then spun at 8, 10, and 15wt% concentration in a single chlor<strong>of</strong>orm solvent, respective. The<br />
lactide-based polymeric nan<strong>of</strong>ibers were characterized by Scanning Electron Microscope (SEM). Smooth surface morphology<br />
was observed in nan<strong>of</strong>ibers produced from PLLA and 50% P(LLA-co-DLLA) copolymer. However, surface porosity was<br />
observed in the corresponding fibers from 2.5 and 7.5% P(LLA-co-DLLA) copolymers. These nan<strong>of</strong>ibers have high potential for<br />
wide range <strong>of</strong> applications such as filter media, nano-sensor, drug delivery and tissue scaffold, especially, those derived from<br />
2.5 and 7.5% P(LLA-co-DLLA) copolymers which contain high degree <strong>of</strong> porosity © (<strong>2010</strong>) Trans Tech <strong>Publications</strong>.<br />
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No.1496<br />
Author (s) : Thamwattana, N., Hill, J.M., Baowan, D., Cox, B.J.<br />
Title : A review <strong>of</strong> mathematical and mechanical modelling in nanotechnology<br />
Source : Mathematics and Mechanics <strong>of</strong> Solids, Volume 15, Issue 7, September <strong>2010</strong>, Pages 708-717<br />
Document Type : Review<br />
Keywords : Bionnanotechnolgy; Gas Separation; Hydrogen Storage; Mathematical Modelling; Mechanics; Molecular<br />
Structure; Nanomedicine<br />
<strong>Abstract</strong> : Nanotechnology is currently an area <strong>of</strong> intense research activity. To date there is some limited experimental data, and<br />
considerably more computational data arising from molecular dynamics simulations, but there is very little applied<br />
mathematical modelling from the disciplines associated with modelling, namely mathematics and mechanics. This paper<br />
reviews some <strong>of</strong> the mathematical and mechanical modelling contributions for interacting molecular structures. Modelling<br />
can indicate to the experimentalist materials and geometry where a certain outcome may be anticipated. A combined<br />
modelling-computational approach can make efficient a computational procedure that alone is not practical. The present<br />
review aims to provide an overview <strong>of</strong> contributions to date, but is necessarily focussed on the work <strong>of</strong> the present authors,<br />
since the number <strong>of</strong> mathematically orientated workers in the field is limited. © The Author(s), <strong>2010</strong>.<br />
No.1497<br />
Author (s) : Thamwiriyasati, N., Powthongchin, B., Kittiworakarn, J., Katzenmeier, G., Angsuthanasombat, C.<br />
Title : Esterase activity <strong>of</strong> Bordetella pertussis CyaC-acyltransferase against synthetic substrates: Implications for catalytic mechanism<br />
in vivo<br />
Source : FEMS Microbiology Letters, Volume 304, Issue 2, March <strong>2010</strong>, Pages 183-190<br />
Document Type : Article<br />
Keywords : Acyltransferase; Adenylate cyclase-hemolysin; Bordetella pertussis; Fatty-acyl modification; In vitro activation; Serine<br />
esterase<br />
<strong>Abstract</strong> : Adenylate cyclase-hemolysin toxin (CyaA) produced from the human respiratory tract pathogen Bordetella pertussis requires<br />
fatty-acyl modification by CyaC-acyltransferase to become an active toxin. Previously, the recombinant CyaA pore-forming<br />
(CyaA-PF) fragment expressed in Escherichia coli was shown to be hemolytically active upon palmitoylation in vivo by<br />
cosynthesized CyaC. Here, the 21-kDa CyaC enzyme separately expressed in E. coli as an inclusion body was solubilized in 8<br />
M urea and successfully refolded into an enzymatically active monomer. In addition to the capability <strong>of</strong> activating CyaA-PF in<br />
vitro, CyaC showed esterase activity against p-nitrophenyl acetate (pNPA) and p-nitrophenyl palmitate (pNPP), with<br />
preferential hydrolysis toward pNPP when compared with chymotrypsin. A homology-based CyaC structure suggested a<br />
conceivable role <strong>of</strong> a catalytic triad including Ser30, His33 and Tyr 66 in substrate catalysis. Alanine substitutions <strong>of</strong> these<br />
individual residues caused a drastic decrease in specific activities <strong>of</strong> all three mutant enzymes (S30A, H33A and Y66A) toward<br />
pNPP, signifying that CyaC-acyltransferase shares a similar mechanism <strong>of</strong> hydrolysis with a serine esterase in which Ser30 is<br />
part <strong>of</strong> the catalytic triad. © <strong>2010</strong> Federation <strong>of</strong> European Microbiological Societies. Published by Blackwell Publishing Ltd. All<br />
rights reserved.<br />
No.1498<br />
Author (s) : Thamwiriyasati, N., Sakdee, S., Chuankhayan, P., Katzenmeier, G., Chen, C.-J., Angsuthanasombat, C.<br />
Title : Crystallization and preliminary X-ray crystallographic analysis <strong>of</strong> a full-length active form <strong>of</strong> the Cry4Ba toxin from Bacillus<br />
thuringiensis<br />
Source : Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Volume 66, Issue 6, <strong>2010</strong>, Pages 721-<br />
724<br />
Document Type : Article<br />
Keywords : Bacillus thuringiensis; Cry4Ba mosquito-larvicidal protein<br />
<strong>Abstract</strong> : To obtain a complete structure <strong>of</strong> the Bacillus thuringiensis Cry4Ba mosquito-larvicidal protein, a 65 kDa functional form <strong>of</strong><br />
the Cry4Ba-R203Q mutant toxin was generated for crystallization by eliminating the tryptic cleavage site at Arg203. The 65 kDa<br />
trypsin-resistant fragment was purified and crystallized using the sitting-drop vapour-diffusion method. The crystals belonged<br />
to the rhombohedral space group R32, with unit-cell parameters a = b = 184.62, c = 187.36 Å. Diffraction data were collected<br />
to at least 2.07 Å resolution using synchrotron radiation and gave a data set with an overall R merge <strong>of</strong> 9.1% and a<br />
completeness <strong>of</strong> 99.9%. Preliminary analysis indicated that the asymmetric unit contained one molecule <strong>of</strong> the active full-<br />
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length mutant, with a V M coefficient and solvent content <strong>of</strong> 4.33 Å3 Da-1 and 71%, respectively. © <strong>International</strong> Union <strong>of</strong><br />
Crystallography <strong>2010</strong>.<br />
No.1499<br />
Author (s) : Thanapprapasr, D., Chittithaworn, S., Lertkhachonsuk, A.-A., Udomsubpayakul, U., Wilailak, S.<br />
Title : Female hospital-based healthcare pr<strong>of</strong>essionals' knowledge <strong>of</strong> cervical cancer, HPV and attitudes towards HPV vaccination<br />
Source : Asian Pacific Journal <strong>of</strong> Cancer Prevention, Volume 11, Issue 2, <strong>2010</strong>, Pages 429-433<br />
Document Type : Article<br />
Keywords : Cervical cancer; HPV; Knowledge<br />
<strong>Abstract</strong> : Objective: To find out female hospital-based healthcare pr<strong>of</strong>essionals' knowledge <strong>of</strong> cervical cancer, HPV and attitudes<br />
towards HPV vaccination. Design: A descriptive cross-sectional hospital-based study. Setting: Ramathibodi Hospital, <strong>Mahidol</strong><br />
<strong>University</strong>, Bangkok, Thailand. Sample: A total <strong>of</strong> 350 female hospital-based healthcare pr<strong>of</strong>essionals who had not been<br />
diagnosed as having any cancer and willing to participate in the study. Methods: Participants completed written consent and<br />
an anonymous questionnaire and knowledge <strong>of</strong> cervical cancer, HPV and attitudes towards HPV vaccination were the main<br />
outcome measures. Results: Among 300 responders, the mean age was 36.1 years. Most <strong>of</strong> them were married with children<br />
and had received university education. Nursing assistants accounted for 47.1%, and their income per month was about 5,001-<br />
15,000 baht. Most (56.3%) had only one lifetime sexual partner. Sixty-eight to 85.3% have a good knowledge <strong>of</strong> cervical<br />
cancer and Pap smear. However, only 12.0 to 58.3% have some knowledge <strong>of</strong> HPV, and less than fifty percent <strong>of</strong> them have<br />
knowledge <strong>of</strong> HPV vaccination. Nevertheless, 51.7 to 60.7% <strong>of</strong> them have good attitudes toward vaccination. Their age and<br />
income might influence their attitudes about having themselves vaccinated, and their career might be a factor which altered<br />
their attitudes about having their daughter vaccinated if they have one. Conclusions: Female hospital-based healthcare<br />
pr<strong>of</strong>essionals have a good knowledge about cervical cancer and Pap smears, but they need motivation to have Pap tests<br />
regularly. More information regarding HPV and vaccination is needed to provide to them for cervical cancer prevention and<br />
best practices.<br />
No.1500<br />
Author (s) : Thanapprapasr, D., Nartthanarung, A., Likittanasombut, P., Ayudhya, N.I.N., Charakorn, C., Udomsubpayakul, U.,<br />
Subhadarbandhu, T., Wilailak, S.<br />
Title : Bone metastasis in cervical cancer patients over a 10-year period<br />
Source : <strong>International</strong> Journal <strong>of</strong> Gynecological Cancer, Volume 20, Issue 3, April <strong>2010</strong>, Pages 373-378<br />
Document Type : Article<br />
Keywords : Bone metastasis; Cervical cancer<br />
<strong>Abstract</strong> : Introduction: Cervical cancer is the major cancer burden in developing countries. Bone is the third most common site <strong>of</strong><br />
distant metastasis after the lungs and liver. Therefore, the aims <strong>of</strong> this study were to find the incidence and clinical<br />
characteristics <strong>of</strong> bone metastasis in our hospital. Patients and Methods: Fifty-one cervical cancer patients with bone<br />
metastasis during the period from January 1998 to December 2007 were recruited. All patients' medical records were<br />
reviewed and analyzed. Results: Among 4620 cervical cancer patients, there were 51 patients (1.1%) who had bone<br />
metastases. Ten patients' medical records were not found; thus, 41 patients were available for evaluation. The median age <strong>of</strong><br />
the patients was 49 years. <strong>International</strong> Federation <strong>of</strong> Gynecology and Obstetrics stage IIB was the most common stage<br />
(43.9%). Most patients had squamous cell carcinoma (80.48%) and received radiation therapy alone as their primary<br />
treatment (58.53%). The most common presenting symptom was pain (78.04%). Most <strong>of</strong> the patients had multiple bone<br />
lesions and extrapelvic bone metastases. The lumbar spine was the most common site (36.36%). Sixteen patients (39.02%)<br />
were treated by palliative radiation therapy. The median overall survival was 23 months. Conclusions: Bone metastases could<br />
be found at all stages. Common sites were the bone beyond the radiation field <strong>of</strong> their primary treatment. It was found at a<br />
median <strong>of</strong> 16 months after cervical cancer diagnosis. Currently, there are many varieties <strong>of</strong> treatment that result only in<br />
palliation. This group <strong>of</strong> patients has a poor prognosis. Copyright © <strong>2010</strong> by IGCS and ESGO.<br />
No.1501<br />
Author (s) : Thanapprapasr, D.; Wilailak, S.; Ayudhya, NIN; Lertkhachonsuk, AA; Likittanasombut, P.; Chittithaworn, S.; Charakorn, C.;<br />
Weerakiet, S.<br />
Title : Can Vaginal Misoprostol Effectively Increase Rate <strong>of</strong> a Satisfactory Colposcopy? A Randomized Double-blind Placebocontrolled<br />
Trial*<br />
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Source : JAPANESE JOURNAL OF CLINICAL ONCOLOGY, Volume: 40, Issue: 3, Pages: 203-207, DOI: 10.1093/jjco/hyp140, Published: MAR<br />
<strong>2010</strong><br />
Document Type : Article<br />
Keywords : misoprostol; unsatisfactory colposcopy<br />
<strong>Abstract</strong> : To evaluate the effectiveness <strong>of</strong> vaginal misoprostol in overcoming an unsatisfactory colposcopy in the patients who had<br />
abnormal cervical cytology and to evaluate side effects <strong>of</strong> vaginal misoprostol. Sixty patients with an unsatisfactory<br />
colposcopy during the period <strong>of</strong> September 2007-November 2008 were recruited and randomly allocated to receive either<br />
two tablets <strong>of</strong> 200 mu g misoprostol (400 mu g) or two tablets <strong>of</strong> similar-looking placebo vaginally. Colposcopic reexamination<br />
was performed similar to 6 h later. The results and side effects before and 2 weeks after the colposcopic reexamination<br />
were recorded. Six out <strong>of</strong> 30 patients in the misoprostol group (20.0%) had a satisfactory colposcopic reexamination<br />
compared with 2 out <strong>of</strong> 27 patients (7.4%) in the placebo group without statistically significant difference (P =<br />
0.172). Three patients in the placebo group dropped out due to not present at the appointment time. Six out <strong>of</strong> 30 patients<br />
(20.0%) and 1 out <strong>of</strong> 30 patients (3.3%) in the misoprostol group had side effects before and 2 weeks after the colposcopic<br />
re-examination orderly. Twenty-seven patients in the placebo group did not have any side effects before and 2 weeks after<br />
the colposcopic re-examination. All side effects occurred were minimal and well tolerated. Four hundred micrograms <strong>of</strong><br />
vaginal misoprostol were not proved to be effective in converting an unsatisfactory to a satisfactory colposcopy.<br />
No.1502<br />
Author (s) : Thanasai, J., Limpaiboon, T., Jearanaikoon, P., Sripa, B., Pairojkul, C., Tantimavanich, S., Miwa, M.<br />
Title : Effects <strong>of</strong> thymidine phosphorylase on tumor aggressiveness and 5-fluorouracil sensitivity in cholangiocarcinoma<br />
Source : World Journal <strong>of</strong> Gastroenterology, Volume 16, Issue 13, 7 April <strong>2010</strong>, Pages 1631-1638<br />
Document Type : Article<br />
Keywords : 5-fluorouracil; Cell migration; Cholangiocarcinoma; Liver fluke; siRNA; Thymidine phosphorylase; Tumor aggressive-ness<br />
<strong>Abstract</strong> : Aim: To evaluate the role <strong>of</strong> thymidine phosphorylase (TP) in cholangiocarcinoma using small interfering RNA (siRNA).<br />
Methods: A human cholangiocarcinoma-derived cell line KKU-M139, which has a naturally high level <strong>of</strong> endogenous TP, had<br />
TP expression transiently knocked down using siRNA. Cell growth, migration, in vitro angiogenesis, apoptosis, and cytotoxicity<br />
were assayed in TP knockdown and wild-type cell lines. Results: TP mRNA and protein expression were decreased by 87.1% ±<br />
0.49% and 72.5% ± 3.2%, respectively, compared with control cells. Inhibition <strong>of</strong> TP significantly decreased migration <strong>of</strong> KKU-<br />
M139, and suppressed migration and tube formation <strong>of</strong> human umbilical vein endothelial cells. siRNA also reduced the ability<br />
<strong>of</strong> TP to resist hypoxia-induced apoptosis, while suppression <strong>of</strong> TP reduced the sensitivity <strong>of</strong> KKU-M139 to 5-fluorouracil.<br />
Conclusion: Inhibition <strong>of</strong> TP may be beneficial in decreasing angiogenesis-dependent growth and migration <strong>of</strong><br />
cholangiocarcinoma but may diminish the response to 5-fluorouracil chemotherapy. © <strong>2010</strong> Baishideng.<br />
No.1503<br />
Author (s) : Thanasak, J., Jittakhot, S., Kosulwat, S., Rukkwamsuk, T.<br />
Title : Fatty acid pr<strong>of</strong>ile <strong>of</strong> ruminal fluid, plasma and milk fat <strong>of</strong> dairy cows fed soybean and sunflower oil-rich diets, without effects<br />
on milk production<br />
Source : Kasetsart Journal - Natural Science, Volume 44, Issue 5, September <strong>2010</strong>, Pages 837-849<br />
Document Type : Article<br />
Keywords : Cow; Milk; Polyunsaturated fatty acid; Soybean oil; Sunflower oil<br />
<strong>Abstract</strong> : The objective <strong>of</strong> the study was to evaluate the effect <strong>of</strong> feeding soybean oil (SB) and sunflower oil (SF) on ruminal fluid,<br />
plasma and milk fatty acid pr<strong>of</strong>iles. Eighteen cows were obtained for the experiment and received 20 kg corn silage and 7.5 kg<br />
concentrate as a base diet, containing 6.2% palm oil on a dry matter basis, for four weeks. Subsequently, after the four-week<br />
experimental period, six cows were assigned to each <strong>of</strong> the dietary treatments. The treatments consisted <strong>of</strong> three groups: 1)<br />
the same base diet, containing 6.2% palm oil (control); 2) 6.2% SB; and 3) 6.2% SF. All treatments did not alter milk<br />
production. The milk fat percentages were significantly (P < 0.05) lower in the cows fed the SB- and SF-rich diets at day 7 and<br />
14, whereas milk fat yields were significantly (P < 0.05) lower only in the cows fed the SF-rich diet at day 7. The proportion <strong>of</strong><br />
ruminal C16:0 was significantly (P < 0.001) lower and C 18:0 was significantly (P < 0.05) higher for the SB and SF diets, while in<br />
addition, C18: ln-9 tended to be lower on the SF diet (P = 0.062). The proportions <strong>of</strong> plasma C16:0 and C18: ln-9 were<br />
significantly (P < 0.05) lower for the SB and SF diets, whereas CI8:0 was significantly (P = 0.027) higher with the SB diet. Even<br />
though no differences in polyunsaturated fatty acids, in either the ruminal fluid or plasma, were found among the treatments,<br />
the proportion <strong>of</strong> milk linoleic acids (C18:2n-6) was similar in the SB and SF diets and both diets had proportions that were<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
significantly (P < 0.001) higher than in the control. In addition, milk linolenic acids (C18:3n-3) were significantly (P < 0.05)<br />
greater in the SB diet than for the SF diet and for the control. An increase in the ruminal pH and a reduction in blood nonesterified<br />
fatty acid (NEFA) were detected in cows fed either the SB or SF diet. The results suggested (with the balance<br />
between dietary fatty acids hydrogenated in the rumen and taken up by the mammary gland) that SB is an acceptable fat<br />
source for high linoleic acid and linolenic acid (omega-6 and 3) and that SF is suitable for high linoleic acid (omega-6)produced<br />
milk, without adverse effects on milk yield and composition. This practical feeding trial would reflect the use <strong>of</strong><br />
these oils to produce health-enhancing dairy products.<br />
No.1504<br />
Author (s) : Thani, W., Vallisuta, O., Siripong, P., Ruangwises, N.<br />
Titel : Anti-proliferative and antioxidative activities <strong>of</strong> Thai noni/Yor (Morinda citrifolia Linn.) leaf extract<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 2, March <strong>2010</strong>, Pages 482-489<br />
Document Type : Article<br />
Keywords : Anti-oxidative activity; Anti-proliferative effects; Cancer cell lines; Leaf extract; Morinda citrifolia Linn.; Thai noni<br />
<strong>Abstract</strong> : In this study the leaves <strong>of</strong> the Thai noni/Yor, (Morinda citrifolia Linn.) were extracted by several methods and evaluated<br />
against human cancer cell lines: KB (human epidermoid carcinoma), HeLa (human cervical carcinoma), MCF-7 (human breast<br />
carcinoma) and HepG 2 (human hepatocellular carcinoma) cell lines as well as a Vero (African green monkey kidney) cell line,<br />
employing the MTT colorimetric method, comparing it to damnacanthal, rutin, and scopoletin. The dichloromethane extract<br />
<strong>of</strong> the fresh leaf showed a better inhibitory effect against KB and HeLa cells with IC 50 values <strong>of</strong> 21.67 and 68.50 μg/ml,<br />
respectively. The dichloromethane extract <strong>of</strong> dried leaves revealed cytotoxicity against the KB cell line with an IC 50 value <strong>of</strong><br />
39.00 μg/ml. Other extracts, as well as rutin and scopoletin, showed reduced anti-proliferative effects on all cancer cell lines<br />
(IC 50 103 to over 600 μg/ml). Interestingly, the damnacanthal had potent cytotoxicity against all cancer cell lines and Vero<br />
cell lines. These results suggest Thai noni extracts may be safer than the pure compounds, due to their higher safety ratios,<br />
which is a good indicator for possible cancer treatment. Several non-aqueous extracts from the leaves showed antioxidant<br />
properties, giving IC 50 values <strong>of</strong> 0.20-0.35 mg/ml. It can be concluded the leaves <strong>of</strong> M. citrifolia may have benefit as a food<br />
supplement for chemoprevention against epidermoid and cervical cancers.<br />
No.1505<br />
Author (s) : Thanongsaksrikul, J., Srimanote, P., Maneewatch, S., Choowongkomon, K., Tapchaisri, P., Makino, S.-I., Kurazono, H., Chaicumpa,<br />
W.<br />
Title : AVHH that neutralizes the zinc metalloproteinase activity <strong>of</strong> botulinum neurotoxin type A<br />
Source : Journal <strong>of</strong> Biological Chemistry, Volume 285, Issue 13, 26 March <strong>2010</strong>, Pages 9657-9666<br />
Document Type : Article<br />
Keywords : Amino acid residues, Amino acid sequence, Antibody fragment, Antibody molecules, Botulinum neurotoxin type A, E.<br />
coli, Further development, Heavy-chain antibody fragments, Hydrolytic activities, Immunoglobulin genes, Light<br />
chain, Metalloproteinase activity, Molecular docking, Phage clones, Phage display libraries, Polyclonal, Therapeutic agents;<br />
Amplification, Antibodies, Cloning, Escherichia coli, Organic acids, Zinc; Amino acids; botulinum toxin<br />
A, epitope, immunoglobulin fragment, immunoglobulin heavy chain, immunoglobulin heavy chain<br />
fragment, metalloproteinase, synaptosomal associated protein 25, unclassified drug, zinc<br />
metalloproteinase, antibody, peptide library, recombinant protein, zinc; amino acid sequence, animal cell, antigen<br />
specificity, article, biodegradation, camel, catalysis, complementarity determining region, controlled study, DNA<br />
sequence, enzyme activity, gene construct, gene location, hydrolysis, male, molecular cloning, molecular<br />
docking, nonhuman, nucleotide sequence, phage display, priority journal, toxin<br />
analysis, animal, chemistry, human, immunoglobulin variable region, Institute for Cancer Research<br />
mouse, metabolism, molecular genetics, mouse, protein conformation, sequence homology; Animalia, Camelus dromedarius;<br />
Amino Acid Sequence, Animals, Antibodies, Botulinum Toxin Type A, Camels, Humans, Immunoglobulin Variable<br />
Region, Male, Metalloproteases, Mice, Mice, Inbred ICR, Molecular Sequence Data, Peptide Library, Protein<br />
Conformation, Recombinant Proteins, Sequence Homology, Amino Acid, Zinc<br />
<strong>Abstract</strong> : The current treatment <strong>of</strong> botulism is to administer animal-derived antitoxin, which frequently causes severe adverse reactions<br />
in the recipients. In this study, a heavy chain antibody fragment (VH/VHH) phage display library was constructed by<br />
amplification <strong>of</strong> the immunoglobulin genes <strong>of</strong> a nonimmune camel, Camelus dromedarius, using primers specific to human<br />
VH gene segments. A recombinant light chain <strong>of</strong> type A botulinumtoxin, BoTxA/LC, with zinc endoprotease activity was used<br />
547
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
in phage bio-panning to select phage clones displaying BoTxA/LC-bound VH/VHH. Soluble VH/VHHwere produced and purified<br />
from 10 VH/VHH phagemid-transformed E. coli clones. Complementary determining regions (CDRs) and immunoglobulin<br />
frameworks (FRs) <strong>of</strong> the 10 camel VH/VHH-deduced amino acid sequences were determined. FR2 sequences <strong>of</strong> two clones<br />
showed a hallmark <strong>of</strong> camel V HH, i.e. (F/Y)42E49R50(G/F) 52. The remaining eight clones had an FR2 amino acid tetrad <strong>of</strong><br />
conventional VH, i.e. V42G49L50W52. VHH <strong>of</strong> one clone (VHH17) neutralized the SNAP25 hydrolytic activity <strong>of</strong> BoTxA/LC,<br />
whereas mouse polyclonal anti-BoTxA/LC did not have such activity. Mimotope sequences <strong>of</strong> VHH17 matched with the 194-<br />
206 amino acid residues <strong>of</strong> BoTxA/LC, which are located near the S′1 subsite <strong>of</strong> the catalytic cleft <strong>of</strong> the enzyme. Molecular<br />
docking revealed that CDR3 <strong>of</strong> the VHH17 bound to epitope in the toxin enzymatic cleft. Therefore, the BoTxA/LC<br />
neutralization by the VHH17 should be due to the V HH insertion into the enzymatic cleft <strong>of</strong> the toxin, which is usually<br />
inaccessible to a conventional antibody molecule. This antibody fragment warrants further development as a therapeutic<br />
agent for botulism. © <strong>2010</strong> by The American Society for Biochemistry and Molecular Biology, Inc.<br />
No.1506<br />
Author (s) : Thant, M.M., Charmondusit, K.<br />
Title : Eco-efficiency assessment <strong>of</strong> pulp and paper industry in Myanmar<br />
Source : Clean Technologies and Environmental Policy, Volume 12, Issue 4, August <strong>2010</strong>, Pages 427-439<br />
Document Type : Article<br />
Keywords : Eco-efficiency . Pulp and paper industry Economic pr<strong>of</strong>ile . Environmental pr<strong>of</strong>ile<br />
<strong>Abstract</strong> : This paper presents the eco-efficiency assessment <strong>of</strong> the pulp and paper industry in Myanmar by using the key indicators<br />
such as raw material consumption, energy consumption, total waste output, water consumption, and CO2 emissions. The<br />
study was carried out by using quantitative methods for data analysis <strong>of</strong> the production, consumptions and emissions from<br />
fiscal year 2001-2005. The results revealed that the level <strong>of</strong> economic and environmental performance using the ecoefficiency<br />
ratio for each fiscal year has decreased since year 2002, and factory tried to increase the level <strong>of</strong> eco-efficiency<br />
again in year 2005. There was the positive aspect that factory could optimize the waste utilization by transferring lime mud to<br />
the cement factory in the last two fiscal years. This analysis showed the root causes that led to the losses <strong>of</strong> material, energy<br />
and water consumption and discussed how to conserve those utilities. © Springer-Verlag 2009.<br />
No.1507<br />
Author (s) : Thapa, B., Tribuddharat, C., Srifuengfung, S., Dhiraputra, C.<br />
Title : High prevalence <strong>of</strong> BLAOXA-23 in oligoclonal carbapenem-resistant acinetobacter baumannii from siriraj hospital, <strong>Mahidol</strong><br />
<strong>University</strong>, Bangkok, Thailand<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 3, May <strong>2010</strong>, Pages 625-635<br />
Document Type : Article<br />
Keywords : β-lactamase gene; A. baumannii; Carbapenem resistance; CRAB<br />
<strong>Abstract</strong> : Acinetobacter baumannii has emerged in health care settings as a pandrugresistant pathogen. Carbapenems are ineffective for<br />
treatment <strong>of</strong> this pathogen. Here we explored the molecular epidemiology and mechanism <strong>of</strong> carbapenem resistance in<br />
clinical isolates <strong>of</strong> carbapenem-resistant A. baumannii (CRAB). Antibiotic susceptibility by disk diffusion test was performed<br />
using imipenem and meropenem disk on 200 different clinical CRAB isolates. All isolates were resistant and gave inhibition<br />
zones <strong>of</strong> both antibiotic disks ≤13 mm. Polymerase chain reaction (PCR) was carried out on 37 randomly selected isolates to<br />
amplify the common carbapenem hydrolyzing β-lactamase genes (blaOXA-23-like, blaOXA-24/40like, blaOXA-58, blaIMP, and<br />
blaVIM). Clones were resolved by PCR-randomly amplified polymorphic DNA (PCR-RAPD) and plasmid pr<strong>of</strong>iling. PCR<br />
amplification and DNA sequencing revealed the existence <strong>of</strong> blaOXA-23 downstream <strong>of</strong> the insertion element, ISAba1, in all<br />
37 isolates tested. This segment was present in the carbapenem-resistant genomic resistant island AbaR4. These isolates were<br />
resolved into three RAPD types (Type I, 20 isolates; Type II, 16 isolates; and type III, 1 isolate) and 10 plasmid pr<strong>of</strong>iles. The<br />
CRAB isolates investigated here were oligoclonal and carbapenem resistance was conferred by the presence <strong>of</strong> blaOXA-23.<br />
The presence <strong>of</strong> this β-lactamase gene in many clonal isolates indicated its wide spread.<br />
No.1508<br />
Author (s) : Thasana, N., Prapagdee, B., Rangkadilok, N., Sallabhan, R., Aye, S.L., Ruchirawat, S., Loprasert, S.<br />
Title : Bacillus subtilis SSE4 produces subtulene A, a new lipopeptide antibiotic possessing an unusual C15 unsaturated β-amino<br />
acid<br />
Source : FEBS Letters, Volume 584, Issue 14, July <strong>2010</strong>, Pages 3209-3214<br />
548
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Document Type : Article<br />
Keywords : Antimicrobial activity; Cyclic lipopeptide; Iturin family; Structure elucidation<br />
<strong>Abstract</strong> : Subtulene A, a new cyclic lipopeptide, was isolated from the culture broth <strong>of</strong> Bacillus subtilis SSE4. This antibiotic compound<br />
contained the seven common α-amino acids, l-Asn-1, d-Tyr-2, d-Asn-3, l-Gln-4, l-Pro-5, d-Asn-6, l-Ser-7 and the unique βamino<br />
acid-8 present in the iturin family. 1D and 2D NMR, as well as MS analyses, identified the β-amino acid as 3-amino-13methyltetradec-8-enoic<br />
acid, an Iso C15 long chain β-amino acid. B. subtilis SSE4 was also found to produce iturin A. B.<br />
subtilis SSE4 culture filtrate exhibited both antifungal and antibacterial activities. © <strong>2010</strong> Federation <strong>of</strong> European Biochemical<br />
Societies.<br />
No.1509<br />
Author (s) : Thavorncharoensap, M., Teerawattananon, Y., Yothasamut, J., Lertpitakpong, C., Thitiboonsuwan, K., Neramitpitagkul, P.,<br />
Chaikledkaew, U.<br />
Title : The economic costs <strong>of</strong> alcohol consumption in Thailand, 2006<br />
Source : BMC Public Health, Volume 10, <strong>2010</strong>, Article number 323<br />
Document Type : Article<br />
Keywords : –<br />
<strong>Abstract</strong> : Background. There is evidence that the adverse consequences <strong>of</strong> alcohol impose a substantial economic burden on societies<br />
worldwide. Given the lack <strong>of</strong> generalizability <strong>of</strong> study results across different settings, many attempts have been made to<br />
estimate the economic costs <strong>of</strong> alcohol for various settings; however, these have mostly been confined to industrialized<br />
countries. To our knowledge, there are a very limited number <strong>of</strong> well-designed studies which estimate the economic costs <strong>of</strong><br />
alcohol consumption in developing countries, including Thailand. Therefore, this study aims to estimate these economic<br />
costs, in Thailand, 2006. Methods. This is a prevalence-based, cost-<strong>of</strong>-illness study. The estimated costs in this study included<br />
both direct and indirect costs. Direct costs included health care costs, costs <strong>of</strong> law enforcement, and costs <strong>of</strong> property<br />
damage due to road-traffic accidents. Indirect costs included costs <strong>of</strong> productivity loss due to premature mortality, and costs<br />
<strong>of</strong> reduced productivity due to absenteeism and presenteeism (reduced on-the-job productivity). Results. The total economic<br />
cost <strong>of</strong> alcohol consumption in Thailand in 2006 was estimated at 156,105.4 million baht (9,627 million US$ PPP) or about<br />
1.99% <strong>of</strong> the total Gross Domestic Product (GDP). Indirect costs outweigh direct costs, representing 96% <strong>of</strong> the total cost. The<br />
largest cost attributable to alcohol consumption is that <strong>of</strong> productivity loss due to premature mortality (104,128 million<br />
baht/6,422 million US$ PPP), followed by cost <strong>of</strong> productivity loss due to reduced productivity (45,464.6 million baht/2,804<br />
million US$ PPP), health care cost (5,491.2 million baht/339 million US$ PPP), cost <strong>of</strong> property damage as a result <strong>of</strong> road<br />
traffic accidents (779.4 million baht/48 million US$ PPP), and cost <strong>of</strong> law enforcement (242.4 million baht/15 million US$<br />
PPP), respectively. The results from the sensitivity analysis revealed that the cost ranges from 115,160.4 million baht to<br />
214,053.0 million baht (7,102.1 - 13,201 million US$ PPP) depending on the methods and assumptions employed.<br />
Conclusions. Alcohol imposes a substantial economic burden on Thai society, and according to these findings, the Thai<br />
government needs to pay significantly more attention to implementing more effective alcohol policies/interventions in order<br />
to reduce the negative consequences associated with alcohol. © <strong>2010</strong> Thavorncharoensap et al; licensee BioMed Central Ltd.<br />
No.1510<br />
Author (s) : Thavorncharoensap, M., Torcharus, K., Nuchprayoon, I., Riewpaiboon, A., Indaratna, K., Ubol, B.-O.<br />
Title : Factors affecting health-related quality <strong>of</strong> life in Thai children with thalassemia<br />
Source : BMC Blood Disorders, Volume 10, 21 January <strong>2010</strong>, Article number 1<br />
Document Type : Article<br />
Keywords : ferritin, hemoglobin, iron chelating agent; academic achievement, adolescent, adult, article, beta thalassemia, blood<br />
transfusion, child, controlled study, disease severity, doctor patient relation, family relation, female, ferritin blood<br />
level, health program, health status, health survey, hemoglobin blood level, human, iron chelation, major clinical<br />
study, male, medical record review, onset age, PedsQL 4.0 Generic Core Scale, psychosocial care, quality <strong>of</strong> life, scoring<br />
system, social psychology, Thailand, thalassemia<br />
<strong>Abstract</strong> : Background: Knowledge <strong>of</strong> the factors associated with health-related quality <strong>of</strong> life (HRQOL) among patients with thalassemia<br />
is essential in developing more suitable clinical, counseling, and social support programs to improve treatment outcomes <strong>of</strong><br />
these patients. In light <strong>of</strong> the limited research in this area, this study aims to examine factors associated with HRQOL among<br />
children and adolescents with thalassemia in Thailand.Methods: A cross-sectional survey was conducted in three selected<br />
hospitals in Thailand during June to November 2006. PedsQL 4.0 Generic Core Scale (Thai version) was used to assess<br />
549
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
HRQOL in 315 thalassemia patients between 5 and 18 years <strong>of</strong> age. Other related clinical characteristics <strong>of</strong> the patients were<br />
collected via medical record review.Results: The mean (SD) <strong>of</strong> the total summary score was 76.67 (11.40), while the means<br />
(SD) for the Physical Health Summary score and Psychosocial Health Summary score were 78.24 (14.77) and 75.54 (12.76),<br />
respectively. The school functioning subscale scored the lowest, with a mean <strong>of</strong> 67.89 (SD = 15.92). The following factors<br />
significantly affected the HRQOL <strong>of</strong> the patients: age; age at onset <strong>of</strong> anemia and age at first transfusion; pre-transfusion<br />
hemoglobin (Hb) level; receiving a blood transfusion during the previous three months; and disease severity. In addition, iron<br />
chelation therapy had a significant negative effect on HRQOL in the school functioning subscale. In contrast, serum ferritin<br />
level, frequency <strong>of</strong> blood transfusions per year, and gender were not significantly related to HRQOL among these patients.<br />
The results from multivariate analysis also confirmed these findings.Conclusions: To improve HRQOL <strong>of</strong> thalassemia patients,<br />
suitable programs aimed at providing psychosocial support and a link between the patient, school <strong>of</strong>ficials, the family and the<br />
physician are important, especially in terms <strong>of</strong> improving the school functioning score. The findings also confirmed the<br />
importance <strong>of</strong> maintaining a pre-transfusion Hb level <strong>of</strong> at least 9-10.5 g/dL. In addition, special care and attention should be<br />
given to patients with a severe condition, and those who are receiving subcutaneous iron chelation therapy. © <strong>2010</strong><br />
Thavorncharoensap et al; licensee BioMed Central Ltd.<br />
No.1511<br />
Author (s) : Thaweboon, B., Laohapand, P., Amornchat, C., Matsuyama, J., Sato, T., Nunez, P.P., Uematsu, H., Hoshino, E.<br />
Title : Host β-globin gene fragments in crevicular fluid as a biomarker in periodontal health and disease<br />
Source : Journal <strong>of</strong> Periodontal Research, Volume 45, Issue 1, February <strong>2010</strong>, Pages 38-44<br />
Document Type : Article<br />
Keywords : Cell damage; Gingival crevicular fluid; Host DNA; Periodontal disease<br />
<strong>Abstract</strong> : Background and Objective: Leukocytes and epithelium are the first line <strong>of</strong> defense in preventing bacterial invasion into<br />
periodontium. Some <strong>of</strong> these cells die in gingival crevicular fluid, whereupon their DNA is spilled out. The present study was<br />
designed to investigate the pr<strong>of</strong>ile <strong>of</strong> host β-globin gene fragments in the gingival crevicular fluid <strong>of</strong> various periodontal<br />
conditions. Material and Methods: Gingival crevicular fluid from 40 teeth with chronic periodontitis, 30 with gingivitis and 22<br />
that were clinically healthy were centrifuged (3000g, 10 min). The supernatant (cell-free gingival crevicular fluid) was<br />
centrifuged again (13,000g, 10 min), resulting in the pellet and the supernatant as debris and debris-free fractions,<br />
respectively. Specific primers for amplifying 110 bp, 536 bp and 2 kb amplicons <strong>of</strong> human β-globin gene were used to<br />
investigate host DNA by quantitative and qualitative polymerase chain reaction. Results: The periodontitis group showed the<br />
largest amount <strong>of</strong> host β-globin gene fragments, while the healthy group had the lowest. In the debris and debris-free<br />
fractions, the 536 bp and 2 kb amplicons were more <strong>of</strong>ten detected in the periodontitis group than in the other groups.<br />
Interestingly, the presence <strong>of</strong> 2 kb amplicon in the debris fraction could be used to discriminate periodontitis from gingivitis<br />
and healthy groups because we found it in 85% <strong>of</strong> periodontitis samples but only in 13% <strong>of</strong> gingivitis samples, and it was<br />
absent in the healthy group. Conclusion: This study shows the different DNA pr<strong>of</strong>iles <strong>of</strong> cell-free gingival crevicular fluid in<br />
periodontal health and disease. It suggests that the quantity and quality <strong>of</strong> host DNA are dependent on the disease<br />
conditions. Therefore, the β-globin gene fragments in cell-free gingival crevicular fluid may be a potential biomarker <strong>of</strong><br />
periodontal disease progression. © 2009 John Wiley & Sons A/S.<br />
No.1512<br />
Author (s) : Thaweethamcharoen, T.; Sakulbumrungsil, R.; Vasuvattakul, S.; Nopmaneejumruslers, C.<br />
Title : COMPARISON OF SF-6D, EQ-5D AND VAS IN HEMODIALYSIS THAI PATIENT<br />
Source : VALUE IN HEALTH, Volume: 13, Issue: 7, Pages: A567-A567, Published: NOV <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1513<br />
Author (s) : Thawornwisit, S., Charmondusit, K., Rempel, G.L., Hinchiranan, N., Prasassarakich, P.<br />
Title : Poly(Methyl Methacrylate-co-Styrene) modified with hydrogenated natural rubber<br />
Source : Journal <strong>of</strong> Elastomers and Plastics, Volume 42, Issue 1, January <strong>2010</strong>, Pages 35-47<br />
Document Type : Article<br />
Keywords : Composite materials; Hydrogenation; Natural rubber; Poly(methyl methacrylate); Styrene<br />
550
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : The mechanical properties <strong>of</strong> brittle polymers are improved by blending with rubbers. Due to C=C in the rubber structure,<br />
polymer/rubber blends have poor thermal and oxidative resistance. In this research, the mechanical and physical properties<br />
including the morphology <strong>of</strong> poly(methyl methacrylate-co-styrene) sheet modified by blending with hydrogenated natural<br />
rubber were investigated. The optimum styrene content in copolymer sheets for improving the mechanical properties was<br />
20% (w/w) and inclusion <strong>of</strong> hydrogenated natural rubber at 1% (w/w) exhibited a higher thermal resistance. This implies that<br />
hydrogenated natural rubber can be used as an impact modifier and thermal resistance improver for acrylic plastics.<br />
No.1514<br />
Author (s) : Theerasasawat, S., Papsing, C., Pulkes, T.<br />
Title : CTG repeat lengths <strong>of</strong> the DMPK gene in myotonic dystrophy patients compared to healthy controls in Thailand<br />
Source : Journal <strong>of</strong> Clinical Neuroscience, Volume 17, Issue 12, December <strong>2010</strong>, Pages 1520-1522<br />
Document Type : Article<br />
Keywords : CTG repeat expansion; DMPK gene; Myotonic dystrophy type 1; Prevalence; Thailand<br />
<strong>Abstract</strong> : Myotonic dystrophy (DM) is frequently associated with large expansions <strong>of</strong> the cytosine-thymine-guanine (CTG) repeat in the<br />
myotonic dystrophy protein kinase gene (DMPK). The frequency <strong>of</strong> distribution <strong>of</strong> the CTG repeat length in normal alleles <strong>of</strong><br />
several populations is well correlated with the prevalence <strong>of</strong> DM. Therefore, we studied the CTG repeat length <strong>of</strong> the DMPK<br />
gene in DM patients and controls in Thailand. Only seven typical patients with DM from six unrelated families were identified,<br />
all with large pathological CTG repeat expansions (>400 repeats) in the DMPK gene. Only 2.75% <strong>of</strong> controls had normal CTG<br />
repeat alleles >18 repeats. The frequency distribution <strong>of</strong> the CTG-repeat alleles in the normal Thai population is similar to<br />
that <strong>of</strong> the Taiwanese population (χ2 with Yates correction = 1.393; p = 0.2379). These data suggest that the incidence <strong>of</strong> DM<br />
might be rare in Thailand, where the risk <strong>of</strong> developing DM is possibly similar to that in Taiwan. © <strong>2010</strong> Elsevier Ltd. All rights<br />
reserved.<br />
No.1515<br />
Author (s) : Theerawit, P., Vongsoasup, A., Kiatboonsri, S.<br />
Title : Identifying non-tuberculous mycobacterium lung diseases in acid-fast bacilli positive patients<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 6, November <strong>2010</strong>, Pages 1432-1437<br />
Document Type : Article<br />
Keywords : Acid-fast bacilli; Identification; Non-tuberculous mycobacterium<br />
<strong>Abstract</strong> : We conducted a case-control study by comparing the clinical and radiographic features <strong>of</strong> acid-fast bacilli positive patients<br />
with pulmonary tuberculosis (PTB) to those with non-tuberculous mycobacterium (NTM) lung diseases diagnosed according to<br />
American Thoracic Society (ATS) criteria. The occurrence <strong>of</strong> NTM lung disease was associated with persistence in symptoms<br />
after the fifth month <strong>of</strong> treatment (p=0.018), middle lobe involvement (p
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : Hyperthyroidism; Iodine-131; Radio-iodine uptake; Radioiodine<br />
<strong>Abstract</strong> : Objective: To compare the effectiveness <strong>of</strong> radioiodine therapy with estimated dose and calculated dose in hyperthyroid<br />
patients. Material and Method: A prospective randomized study in 144 hyperthyroid patients referred for 131I treatment was<br />
performed between June and December 2007. The patients were divided into two groups according to the I dose<br />
administered, estimated group using dose based on gland size and calculated group using dose based on both gland size and<br />
24-hour 131I uptake. Outcome assessment was done at 12 months post-treatment. Results: Eleven <strong>of</strong> 144 patients were<br />
excluded due to loss to follow-up and five became euthyroid before 131I treatment. Fifty-six <strong>of</strong> 128 patients (45.3%)<br />
experienced persistent/recurrent hyperthyroidism, 26 (20.3%) developed hypothyroidism, and 44 (34.4%) were euthyroid.<br />
Outcome was unrelated to the methods <strong>of</strong> I-131 dosing. Only gender and goiter size were found to be correlated with the<br />
clinical outcomes. Conclusion: An estimated I-131 dosing method using gland size determined by palpation is as effective as<br />
calculated method using 131I uptake. This method is more cost effective and brings greater patient convenience.<br />
No.1518<br />
Author (s) : Thiramanas, R., Wanotayan, R., Rahong, S., Jangpatarapongsa, K., Tangboriboonrat, P., Polpanich, D.<br />
Title : Improving malaria diagnosis via latex immunoagglutination assay in micr<strong>of</strong>luidic device<br />
Source : Advanced Materials Research, Volume 93-94, <strong>2010</strong>, Pages 292-295<br />
Document Type : Conference Paper<br />
Keywords : Latex immunoagglutination assay; Malaria diagnosis; Micr<strong>of</strong>luidic device<br />
<strong>Abstract</strong> : Attempt to improve latex immunoagglutination assay, a rapid method in medical diagnostics, reporting as quantitative results<br />
was interested in this study by using micr<strong>of</strong>luidic device. Sensitized latex was produced by physical adsorption <strong>of</strong> human<br />
polyclonal IgG antibody to Plasmodium falciparum malaria parasite onto carboxylated polystyrene particle. Conventional<br />
latex agglutination assay was firstly performed to verify specific interaction <strong>of</strong> antibody on the bead surface versus antigen in<br />
malaria plasma. The agglutinate size around 30 μm was observed under optical microscope. The proportion <strong>of</strong> the plasma<br />
and the particle was optimized, and an appropriate ratio was applied in micr<strong>of</strong>luidic device. Three patterns <strong>of</strong> the device<br />
were used with the agglutinate size comparison after 10 min as followed: rapid mixing > U-shaped loop > straight capillary<br />
Yjunction patterns. However, compared with patient plasma, small agglutinates were also observed when using normal<br />
serum. © (<strong>2010</strong>) Trans Tech <strong>Publications</strong>.<br />
No.1519<br />
Author (s) : Thitilertdecha, P.; Akkarasereenont, P.; Laohapand, T.; Rowan, M. G.; Guy, R.H.<br />
Title : Formulation optimisation for the topical delivery <strong>of</strong> active agents in traditional medicines<br />
Source : JOURNAL OF PHARMACY AND PHARMACOLOGY, Volume: 62, Issue: 10, Special Issue: SI, Pages: 1272-1273, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1520<br />
Author (s) : Thitithanyanont, A., Engering, A., Uiprasertkul, M., Ekchariyawat, P., Wiboon-ut, S., Kraivong, R., Limsalakpetch, A., Kum-Arb, U.,<br />
Yongvanitchit, K., Sa-Ard-Iam, N., Rukyen, P., Mahanonda, R., Kawkitinarong, K., Auewarakul, P., Utaisincharoen, P., Sirisinha, S.,<br />
Mason, C.J., Fukuda, M.M., Pichyangkul, S.<br />
Title : Antiviral immune responses in H5N1-infected human lung tissue and possible mechanisms underlying the hyperproduction <strong>of</strong><br />
interferon-inducible protein IP-10<br />
Source : Biochemical and Biophysical Research Communications, Volume 398, Issue 4, August <strong>2010</strong>, Pages 752-758<br />
Document Type : Article<br />
Keywords : H5N1 autopsy; H5N1-infected human pulmonary cells; IFN-α; IP-10; MxA; TNF-α<br />
<strong>Abstract</strong> : Information on the immune response against H5N1 within the lung is lacking. Here we describe the sustained antiviral<br />
immune responses, as indicated by the expression <strong>of</strong> MxA protein and IFN-α mRNA, in autopsy lung tissue from an H5N1infected<br />
patient. H5N1 infection <strong>of</strong> primary bronchial/tracheal epithelial cells and lung microvascular endothelial cells<br />
induced IP-10, and also up-regulated the retinoic acid-inducible gene-I (RIG-I). Down-regulation <strong>of</strong> RIG-I gene expression<br />
decreased IP-10 response. Co-culturing <strong>of</strong> H5N1-infected pulmonary cells with TNF-α led to synergistically enhanced<br />
production <strong>of</strong> IP-10. In the absence <strong>of</strong> viral infection, TNF-α and IFN-α also synergistically enhanced IP-10 response.<br />
Methylprednisolone showed only a partial inhibitory effect on this chemokine response. Our findings strongly suggest that<br />
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both the H5N1 virus and the locally produced antiviral cytokines; IFN-α and TNF-α may have an important role in inducing<br />
IP-10 hyperresponse, leading to inflammatory damage in infected lung. © <strong>2010</strong>.<br />
No.1521<br />
Author (s) : Thongboonkerd, V.<br />
Title : Current status <strong>of</strong> renal and urinary proteomics: Ready for routine clinical application<br />
Source : Nephrology Dialysis Transplantation, Volume 25, Issue 1, January <strong>2010</strong>, Pages 11-16<br />
Document Type : Note<br />
Keywords : Biomarker discovery; Kidney; Proteome; Proteomics; Urine<br />
<strong>Abstract</strong> : –<br />
No.1522<br />
Author (s) : Thongboonkerd, V.<br />
Title : Proteomics in extracorporeal blood purification and peritoneal dialysis<br />
Source : JOURNAL OF PROTEOMICS, Volume: 73, Issue: 3, Special Issue: SI, Pages: 521-526, DOI: 10.1016/j.jprot.2009.06.003, Published:<br />
JAN 3 <strong>2010</strong><br />
Document Type : Review<br />
Keywords : Acute kidney injury; Artificial organ; Blood purification; Dialysate; ESRD; Hemodialysis; Liver failure; Peritoneal dialysis;<br />
Plasmapheresis; Proteome; Proteomics; Renal replacement therapy<br />
<strong>Abstract</strong> : Extracorporeal blood purification and peritoneal dialysis are widely used in renal replacement therapy for patients with endstage<br />
renal disease (ESRD) and acute kidney injury (AKI). Additionally, extracorporeal blood purification can be used also for<br />
treatment <strong>of</strong> non-renal disorders to remove endogenous or exogenous toxins from the blood circulation. Efforts have been<br />
made to characterize these toxins removed by diffusion (dialysis), convection (ultrafiltration), and/or adsorption (toxins are<br />
adsorbed onto the dialysis membrane and are thus removed) using different types <strong>of</strong> dialysis membrane. This review<br />
summarizes important findings obtained from recent proteomic studies applied to extracorporeal blood purification and<br />
peritoneal dialysis in settings <strong>of</strong> ESRD, AKI and hepatic failure. (C) 2009 Elsevier B.V. All rights reserved.<br />
No.1523<br />
Author (s) : Thonglon, T., Chaikum, N.<br />
Title : Magnetic fingerprint powder from a mineral indigenous to Thailand<br />
Source : Journal <strong>of</strong> Forensic Sciences, Volume 55, Issue 5, September <strong>2010</strong>, Pages 1343-1346<br />
Document Type : Article<br />
Keywords : fingerprint minutiae; fingerprint powder; forensic science; magnetic powder; magnetite; mixed magnetic powder; nickel<br />
powder<br />
<strong>Abstract</strong> : A study was conducted to investigate whether natural magnetite (Fe 3O4), which is an abundant mineral in Thailand, could<br />
be used as a magnetic powder in the detection <strong>of</strong> latent fingerprints. Because <strong>of</strong> the presence <strong>of</strong> impurities, powdered<br />
magnetite is only weakly attracted by a magnet and cannot be used as a magnetic fingerprint powder by itself. Mixing a small<br />
amount <strong>of</strong> magnetite powder with nickel powder greatly enhances the magnetic attraction. A mixture <strong>of</strong> magnetite powder<br />
and nickel powder in a mass ratio <strong>of</strong> approximately 1:100 was found to be suitable for use as a magnetic fingerprint powder.<br />
Fingerprints developed using the magnetite/nickel mixture on nonporous surfaces were found to exhibit good adherence and<br />
clarity. Using an automated fingerprint identification system, the number <strong>of</strong> minutiae detected in fingerprints developed by<br />
using the prepared powder on nonporous surfaces was found to be comparable to those detected in fingerprints developed<br />
by using a commercial black magnetic powder. The cost is lowered by more than 60%. © <strong>2010</strong> American Academy <strong>of</strong><br />
Forensic Sciences.<br />
No.1524<br />
Author (s) : Thong-On, A., Smittipat, N., Juthayothin, T., Yanai, H., Yamada, N., Yorsangsukkamol, J., Chaiprasert, A., Rienthong, D., Billamas,<br />
P., Palittapongarnpim, P.<br />
Title : Variable-number tandem repeats typing <strong>of</strong> Mycobacterium tuberculosis isolates with low copy numbers <strong>of</strong> IS6110 in Thailand<br />
Source : Tuberculosis, Volume 90, Issue 1, January <strong>2010</strong>, Pages 9-15<br />
Document Type : Article<br />
Keywords : Genotyping; IS6110-RFLP; Low-copy-number isolates; Mycobacterium tuberculosis; Spoligotyping; VNTR typing<br />
553
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : Spoligotyping and variable-number tandem repeats (VNTR) typing have been increasingly used for differentiating<br />
Mycobacterium tuberculosis strains with low copy numbers <strong>of</strong> IS6110. However, there are few studies comparing their<br />
potential to type the strains originating from South and Southeast Asia where many <strong>of</strong> the isolates have only a few copies, or<br />
even single copy, <strong>of</strong> IS6110. Here, we evaluated the genotyping <strong>of</strong> 187 M. tuberculosis isolates harboring 1-6 copies <strong>of</strong> IS6110,<br />
available from a population-based study in Chiangrai, northern Thailand during 1998-2000, using spoligotyping and VNTR<br />
typing. The low-copy-number isolates constituted about 34% <strong>of</strong> all M. tuberculosis isolated in the province. Discriminating<br />
capacities and cluster identification by the two methods were compared with each other and to those obtained by the<br />
standard IS6110-restriction fragment length polymorphism (RFLP) method. We found that VNTR typing based on the studied<br />
10-loci set generated more distinct patterns (151 patterns) than spoligotyping (54 patterns) and IS6110-RFLP (65 patterns).<br />
Most <strong>of</strong> the RFLP- or spoligotyping-defined clusters were subdivided by VNTR typing. Combining IS6110-RFLP with VNTR typing<br />
produced 164 distinct patterns and 21.9% <strong>of</strong> clustered isolates whereas the combination <strong>of</strong> IS6110-RFLP and spoligotyping<br />
gave 103 different patterns and 59.4% <strong>of</strong> clustered isolates. Our results confirm the utility <strong>of</strong> VNTR typing as the secondary<br />
method <strong>of</strong> choice for investigating the epidemiology <strong>of</strong> M. tuberculosis with low copy numbers <strong>of</strong> IS6110. © 2009 Elsevier Ltd.<br />
All rights reserved.<br />
No.1525<br />
Author (s) : Thongratsakul, S., Suzuki, Y., Hiramatsu, H., Sakpuaram, T., Sirinarumitr, T., Poolkhet, C., Moonjit, P., Yodsheewan, R., Songserm,<br />
T.<br />
Title : Avian and human influenza a virus receptors in trachea and lung <strong>of</strong> animals<br />
Source : Asian Pacific Journal <strong>of</strong> Allergy and Immunology, Volume 28, Issue 4, December <strong>2010</strong>, Pages 294-301<br />
Document Type : Article<br />
Keywords : Host; Influenza a viruses; Lectin histochemistry; Lung; Receptors; Trachea<br />
<strong>Abstract</strong> : Background: Influenza A viruses are capable <strong>of</strong> crossing the specific barrier between human beings and animals resulting in<br />
interspecies transmission. The important factor <strong>of</strong> potential infectivity <strong>of</strong> influenza A viruses is the suitability <strong>of</strong> the receptor<br />
binding site <strong>of</strong> the host and viruses. The affinities <strong>of</strong> avian and human influenza virus to bind with the receptors and the<br />
distributions <strong>of</strong> receptors in animals are different. Objective: This study aims to investigate the anatomical distribution <strong>of</strong> avian<br />
and human influenza virus receptors using the double staining lectin histochemistry method. Methods: Double staining <strong>of</strong><br />
lectin histochemistry was performed to identify both SA α2,3 Gal and SA α2,6 Gal receptors in trachea and lung tissue <strong>of</strong><br />
dogs, cats, tigers, ferret, pigs, ducks and chickens. Results: We have demonstrated that avian and human influenza virus<br />
receptors were abundantly present in trachea, bronchus and bronchiole, but in alveoli <strong>of</strong> dogs, cats and tigers showed SA<br />
α2,6 Gal only. Furthermore, endothelial cells in lung tissues showed presence <strong>of</strong> SA α2,3 Gal. Conclusion: The positive sites<br />
<strong>of</strong> both receptors in respiratory tract, especially in the trachea, suggest that all mammalian species studied can be infected<br />
with avian influenza virus. These findings suggested that dogs and cats in close contact with humans should be <strong>of</strong> greater<br />
concern as an intermediate host for avian influenza A in which there is the potential for viral adaptation and reassortment.<br />
No.1526<br />
Author (s) : Thongtan, T., Cheepsunthorn, P., Chaiworakul, V., Rattanarungsan, C., Wikan, N., Smith, D.R.<br />
Title : Highly permissive infection <strong>of</strong> microglial cells by Japanese encephalitis virus: a possible role as a viral reservoir<br />
Source : Microbes and Infection, Volume 12, Issue 1, January <strong>2010</strong>, Pages 37-45<br />
Document Type : Article<br />
Keywords : nitric oxide; animal cell, apoptosis, article, cell structure, controlled study, epidemic encephalitis, immun<strong>of</strong>luorescence<br />
microscopy, infection sensitivity, Japanese encephalitis virus, microglia, mouse, neuroblastoma cell, nonhuman, nucleotide<br />
sequence, persistent virus infection, priority journal, virion, virus concentration, virus detection, virus replication;<br />
Animals, Apoptosis, Cell Line, Encephalitis Virus, Japanese, Mice, Microglia, Neurons, Nitric Oxide; Flavivirus, Japanese<br />
encephalitis virus<br />
<strong>Abstract</strong> : Japanese encephalitis virus (JEV), a mosquito-borne Flavivirus, is a major cause <strong>of</strong> acute encephalitis, and neurons have been<br />
proposed to be the principle JEV target cells in the central nervous system. However, clinically, infection with JEV leads to<br />
increased levels <strong>of</strong> cytokines and chemokines in the serum and cerebrospinal fluid (CSF) the levels <strong>of</strong> which correlate with<br />
the mortality rate <strong>of</strong> patients. This research aimed to study the role <strong>of</strong> microglial cells in JEV infection. Mouse microglial cells<br />
(BV-2) supported the replication <strong>of</strong> JEV with extracellular production <strong>of</strong> virus by 10 h post-infection, and virus titer reached a<br />
maximum (2.55 × 1010 pfu/ml) by day 3 post-infection. While apoptosis was induced in response to virus infection, no<br />
alteration in nitric oxide production was observed. Microglial cells remained productively infected with JEV for up to 16 weeks<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
without significant morphological alterations, and the released virions were infectious to mouse neuroblastoma (NA) cells.<br />
The high virus production and long persistence <strong>of</strong> JEV in microglial cells suggests that these cells may serve as viral reservoirs<br />
for the infection <strong>of</strong> neurons in the CNS. © 2009 Elsevier Masson SAS. All rights reserved.<br />
No.1527<br />
Author (s) : Thonpho, A.; Sereeruk, C.; Rojvirat, P.; Jitrapakdee, S.<br />
Title : Identification <strong>of</strong> the cyclic AMP responsive element (CRE) that mediates transcriptional regulation <strong>of</strong> the pyruvate carboxylase<br />
gene in HepG2 cells<br />
Source : BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Volume: 393, Issue: 4, Pages: 714-719, DOI:<br />
10.1016/j.bbrc.<strong>2010</strong>.02.067, Published: MAR 19 <strong>2010</strong><br />
Document Type : Article<br />
Keywords : Pyruvate carboxylase; Gluconeogenesis; Biotin-enzyme; cAMP; cAMP-responsive element binding protein; HepG2<br />
<strong>Abstract</strong> : Pyruvate carboxylase (PC) catalyzes the first committed step in gluconeogenesis. Here we investigated the effect <strong>of</strong> various<br />
hormones including cAMP, dexamethasone and insulin on the abundance <strong>of</strong> PC mRNA in the human hepatocyte cell line,<br />
HepG2. Treatment <strong>of</strong> HepG2 cells with 1 mu M <strong>of</strong> glucagon increased the expression <strong>of</strong> PC mRNA threefold within 72 h.<br />
Treatment with 1 mM 8-Br-cAMP caused the abundance <strong>of</strong> PC mRNA to increase by 2-3-fold by 48 h, peak at fourfold at 72 h,<br />
and remain unchanged to 96 h. This is in contrast to phosphoenolpyruvate carboxykinase (PEPCK) for which expression was<br />
decreased after 72 h, suggesting a distinct difference in the control <strong>of</strong> these two enzymes in the long term. Dexamethasone<br />
or insulin alone did not affect the abundance <strong>of</strong> PC mRNA whereas treatment <strong>of</strong> HepG2 cells with the combination <strong>of</strong> 1 mM<br />
8-Br-cAMP and 0.5 mu M dexamethasone further increased the abundance <strong>of</strong> PC mRNA, suggesting the predominant role <strong>of</strong> 8-<br />
Br-cAMP over dexamethasone. Transient transfection <strong>of</strong> the luciferase reporter construct driven by a 1.95 kbp 5'-flanking<br />
sequence <strong>of</strong> the mouse PC gene and a plasmid encoding the human cAMP-responsive element binding protein increased<br />
luciferase reporter activity to 7-fold similar to that observed with a PEPCK promoter-luciferase reporter construct. Deletion <strong>of</strong><br />
the 5'-flanking region <strong>of</strong> the PC gene to 781 bp resulted in the complete loss <strong>of</strong> CREB-mediated induction <strong>of</strong> reporter gene,<br />
suggesting the presence <strong>of</strong> the cAMP-responsive unit is located between 1.95 kbp and 781 bp upstream <strong>of</strong> the mouse PC<br />
gene. Electrophoretic mobility shifted and chromatin immunoprecipitation assays demonstrated that CREB bind to 1639/-1631<br />
CRE <strong>of</strong> mouse PC gene in vitro and in vivo, respectively. (C) <strong>2010</strong> Elsevier Inc. All rights reserved.<br />
No.1528<br />
Author (s) : Thueng-in, K., Maneewatch, S., Srimanote, P., Songserm, T., Tapchaisri, P., Sookrung, N., Tongtawe, P., Channarong, S.,<br />
Chaicumpa, W.<br />
Title : Heterosubtypic immunity to influenza mediated by liposome adjuvanted H5N1 recombinant protein vaccines<br />
Source : Vaccine, Volume 28, Issue 41, September <strong>2010</strong>, Pages 6765-6777<br />
Document Type : Article<br />
Keywords : Cytokines; Cytotoxic lymphocytes; H1N1; H5N1; Heterosubtypic immunity; Immune<br />
response; Immunization; Influenza; Influenza vaccine; Intramuscular; Liposome; M2 protein; Mice; Nucleoprotein<br />
(NP); RT-PCR<br />
<strong>Abstract</strong> : A non-egg, non-culture based influenza vaccine that intervenes large influenza outbreaks and protects against heterosubtypic<br />
infections is needed. Candidates <strong>of</strong> such vaccine are likely to be conserved influenza virus proteins or their coding DNA. The<br />
vaccine must be conveniently produced at reasonable cost, safe, highly immunogenic and should be able to recall rapidly<br />
the immunological memory upon the antigenic re-exposure. In this study vaccines made <strong>of</strong> full length recombinant NP and<br />
M2 <strong>of</strong> the H5N1 influenza A virus were entrapped either alone or together into liposome (L) made <strong>of</strong> phosphatidylcholine<br />
and cholesterol. The vaccines (L-NP, L-M2 or L-NP+M2) and mocks (L or PBS) were safe without causing any adverse reaction<br />
in the intramuscularly injected mice. They were readily immunogenic at a single dose and a recalled response could be<br />
detected within one day post booster. Cytokine and antibody data indicated that the vaccines induced a Th1 bias immune<br />
response. NP containing vaccines stimulated a marked increase <strong>of</strong> cytotoxic lymphocytes, i.e., CD8+, intracellular IFNγ+ cells,<br />
while M2 containing vaccines elicited good antibody response which neutralized infectivity <strong>of</strong> heterologous influenza viruses.<br />
Although the three vaccines elicited different immunological defense factors; nevertheless, they similarly and readily<br />
abrogated lung histopathology mediated by viruses belonging to different H5N1 clade/subclade and heterosubtypes including<br />
swine H1N1 and human H1N1/2009 viruses. They protected the vaccinated mice against lethal challenges with mouse<br />
adapted avian H5N1 virus. The liposome adjuvanted vaccines which demonstrated high protective efficacy in mice warrant<br />
testing further in a non-rodent model as well as in humans. © <strong>2010</strong> Elsevier Ltd.<br />
555
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No.1529<br />
Author (s) : Thumpimukvatana, N., Wongpraparut, C., Lim, H.W.<br />
Title : Scleredema diabeticorum successfully treated with ultraviolet A1 phototherapy<br />
Source : Journal <strong>of</strong> Dermatology, Volume 37, Issue 12, December <strong>2010</strong>, Pages 1036-1039<br />
Document Type : Article<br />
Keywords : diabetes mellitus; phototherapy; scleredema; ultraviolet A1<br />
<strong>Abstract</strong> : Scleredema diabeticorum is an uncommon condition. It is one <strong>of</strong> the cutaneous manifestations in diabetes mellitus that<br />
mainly occurs in obese middle-aged men with insulin-resistant diabetes. This condition is generally recalcitrant to therapy.<br />
Various treatments have been tried with inconsistent results. Here, we describe two cases <strong>of</strong> scleredema diabeticorum with<br />
substantial clinical improvement from a course <strong>of</strong> medium dose (60 J/cm2) ultraviolet A1 radiation therapy. © <strong>2010</strong> Japanese<br />
Dermatological Association.<br />
No.1530<br />
Author (s) : Tirakunwichcha, S., Rengwanidchakul, E., Asawaphureekorn, S., Tengtrisorn, S., Juangphanich, K., Suramethakul, P., Pornpanich,<br />
K.<br />
Title : Incidence <strong>of</strong> acquired lacrimal drainage system obstruction in epiphoric patients in Thailand<br />
Source : Asian Biomedicine, Volume 4, Issue 1, February <strong>2010</strong>, Pages 159-162<br />
Document Type : Article<br />
Keywords : Dacryocystitis; Epidemiology; Epiphora; Incidence; Lacrimal duct obstruction<br />
<strong>Abstract</strong> : Background: There were few reports regarding the incidence <strong>of</strong> the acquired nasolacrimal duct obstruction , and the<br />
incidence in different geography or countries might be alike. Since the nasolacrimal drainage system obstruction is a common<br />
problem in Thailand, knowledge <strong>of</strong> the incidence may disclose the burden <strong>of</strong> the disease that has never been reported.<br />
Objectives: Evaluate the incidence <strong>of</strong> acquired lacrimal drainage system obstruction (ALDO) in Thailand. Methods: This study<br />
was conducted in multi-centers. It was based on prospective and descriptive design. All patients with epiphoric symptoms<br />
visiting the outpatient departments <strong>of</strong> five tertiary eyecare centers were recruited between 2004 and 2007. Features <strong>of</strong><br />
epiphoric symptoms and medical history were recorded, and complete ophthalmic examinations were performed. Results:<br />
Epiphoric patients were enrolled from all medical centers with 245 eligible patients, female:male ratio was 4.2:1. Ninety-nine<br />
patients (40%) had duration <strong>of</strong> epiphoric symptoms between one-five years. ALDO was found in 111 patients (45%) with<br />
female preponderance (6.9:1). Seventy-one patients (64%) had pre-sac obstruction and 40 patients (36%) had post-sac<br />
obstruction. Among other causes <strong>of</strong> epiphora, dry eye was the most common. Conclusion: The most common cause <strong>of</strong><br />
epiphora was ALDO. The four-year incidence rate <strong>of</strong> ALDO among epiphoric patients was 45%.<br />
No.1531<br />
Author (s) : Tocharus, J., Khonthun, C., Chongthammakun, S., Govitrapong, P.<br />
Title : Melatonin attenuates methamphetamine-induced overexpression <strong>of</strong> pro-inflammatory cytokines in microglial cell lines<br />
Source : Journal <strong>of</strong> Pineal Research, Volume 48, Issue 4, May <strong>2010</strong>, Pages 347-352<br />
Document Type : Article<br />
Keywords : Melatonin; Methamphetamine; Microglia; Pro-inflammatory cytokines<br />
<strong>Abstract</strong> : Methamphetamine (METH), the most commonly abused drug, has long been known to induce neurotoxicity. METH causes<br />
oxidative stress and inflammation, as well as the overproduction <strong>of</strong> both reactive oxygen species (ROS) and reactive nitrogen<br />
species (RNS). The role <strong>of</strong> METH-induced brain inflammation remains unclear. Imbroglio activation contributes to the neuronal<br />
damage that accompanies injury, disease and inflammation. METH may activate microglia to produce neuroinflammatory<br />
molecules. In highly aggressively proliferating immortalized (HAPI) cells, a rat microglial cell line, METH reduced cell viability in<br />
a concentration- and time-dependent manner and initiated the expression <strong>of</strong> interleukin 1β (IL-1β), interleukin 6 (IL-6) and<br />
tumor necrosis factor α. METH also induced the production <strong>of</strong> both ROS and RNS in microglial cells. Pretreatment with<br />
melatonin, a major secretory product <strong>of</strong> the pineal gland, abolished METH-induced toxicity, suppressed ROS and RNS<br />
formation and also had an inhibitory effect on cytotoxic factor gene expression. The expression <strong>of</strong> cytotoxic factors produced<br />
by microglia may contribute to central nervous system degeneration in amphetamine abusers. Melatonin attenuates METH<br />
toxicity and inhibits the expression <strong>of</strong> cytotoxic factor genes associated with ROS and RNS neutralization in HAPI microglia.<br />
Thus, melatonin might be one <strong>of</strong> the neuroprotective agents induced by METH toxicity and/or other immunogens. © <strong>2010</strong><br />
John Wiley & Sons A/S.<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.1532<br />
Author (s) : To-im, J., Tianchai, C., Tianchai, N., Ketpichainarong, W., Jittam, P., Sriwattanarothai, N., Ruenwongsa, P.<br />
Title : Using a local water problem as case-based scenario to encourage thai grade 8 students' learning <strong>of</strong> science<br />
Source : <strong>International</strong> Journal <strong>of</strong> Learning, Volume 17, Issue 6, <strong>2010</strong>, Pages 157-164<br />
Document Type : Article<br />
Keywords : Case-based scenario; Learning; Local water problem; Science<br />
<strong>Abstract</strong> : The study reported here encouraged students to learn about water resource by using a case scenario on local water<br />
problems. After each group <strong>of</strong> students studied the case, students were asked to search for more information on local water<br />
resources, water quality, and comparative quality <strong>of</strong> water. A local sage was invited to give a talk to the students who later<br />
used the knowledge gained to discuss the way to solve the case problems. Students reported their ideas to friends as well as<br />
constructed their own webblog to present what they had learned. Students' presentation, report, webblog, concept map,<br />
pre-post test, and observation were used to assess their achievements from this lesson. The average score was 75.38 from<br />
100 points indicating that most students achieved the objectives <strong>of</strong> the lesson. They could build knowledge from the case,<br />
search for information on their own, and discuss with friends as well as present the understanding via the webblog. Some<br />
problems still need to be solved such as allowing for more time and providing computer each for the student who did not<br />
own one. © Common Ground, Jongdee To-im, Chakpet Tianchai, Nantawadee Tianchai, Watcharee Ketpichainarong, Piyachat<br />
Jittam, Namkang Sriwattanarothai, Pintip Ruenwongsa.<br />
No.1533<br />
Author (s) : Tomita, S., Arphorn, S., Muto, T., Koetkhlai, K., Naing, S.S., Chaikittiporn, C.<br />
Title : Prevalence and risk factors <strong>of</strong> low back pain among Thai and Myanmar migrant seafood processing factory workers in samut<br />
sakorn province, Thailand<br />
Source : Industrial Health, Volume 48, Issue 3, May <strong>2010</strong>, Pages 283-291<br />
Document Type : Conference Paper<br />
Keywords : Cross-sectional study; Food processing worker; Low back pain; Migrant worker; Working condition<br />
<strong>Abstract</strong> : This study assessed the prevalence <strong>of</strong> low back pain (LBP) and investigated risk factors for LBP among seafood processing<br />
factory workers in Thailand including migrant workers. The subjects were Thai and Myanmar workers in the typical seafood<br />
processing factory. A cross-sectional study was carried out with a self-administered questionnaire. Prevalence <strong>of</strong> LBP, general<br />
characteristics, life style, and working condition were investigated. The associations between LBP and risk factors were<br />
estimated by multiple logistic regression models. Of 254 workers, 165 completed the questionnaire. Half <strong>of</strong> these workers<br />
were Thai, the others were from Myanmar. The point prevalence <strong>of</strong> LBP was 28.5%. Risk factors for LBP were age over 40 yr,<br />
poor health status, history <strong>of</strong> back injury, twisting posture at work, and slipping on wet floors. The results suggest that health<br />
promotion should focus on working conditions rather than individual life style in order to prevent LBP. Furthermore, greater<br />
attention to other risk factors such as history <strong>of</strong> back injury and perception <strong>of</strong> health status after regular health check up,<br />
especially in older age groups may be needed.<br />
No.1534<br />
Author (s) : Tongdee, T., Amornvittayachan, O., Tongdee, R.<br />
Title : Accuracy <strong>of</strong> multidetector computed tomography cholangiography in evaluation <strong>of</strong> cause <strong>of</strong> biliary tract obstruction<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 5, May <strong>2010</strong>, Pages 566-573<br />
Document Type : Review<br />
Keywords : Biliary tract obstruction; Jaundice; Multidetector computed tomography cholangiography<br />
<strong>Abstract</strong> : Objective: To evaluate accuracy <strong>of</strong> multidetector computed tomography (MDCT) cholangiography in evaluation <strong>of</strong> cause <strong>of</strong><br />
biliary tract obstruction. Material and Method: MDCT cholangiographs <strong>of</strong> 50 patients with clinically suspected biliary tract<br />
obstruction were retrospectively reconstructed and reviewed. The causes <strong>of</strong> obstruction identified by MDCT were divided into<br />
three groups including calculus, benign stricture, and malignancy. Final diagnosis was based on pathological diagnosis or<br />
endoscopic retrograde cholangiopancreatography or follow-up. The MDCT diagnosis and final diagnosis were compared.<br />
Results: The sensitivity, specificity, positive predictive value, and negative redictive value <strong>of</strong> MDCT cholangiography for<br />
detection <strong>of</strong> calculus, benign stricture, and malignancy were 91.7-100%, except for sensitivity and positive predictive value for<br />
detection <strong>of</strong> benign stricture, which were 66.7% and 66.7% respectively. Conclusion: MDCT cholangiography is a fast,<br />
noninvasive technique that <strong>of</strong>fers high diagnostic accuracy in evaluation <strong>of</strong> cause <strong>of</strong> biliary tract obstruction.<br />
557
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.1535<br />
Author (s) : Tongjaroenbuangam, W.; Govitrapong, P.<br />
Title : EXPRESSION OF NMDA RECEPTOR IN PARAVENTRICULAR NUCLEUS AFTER OKRA (ABELMOSCHUS ESCULENTUS LINN.),<br />
QUERCETIN AND RUTIN TREATED IN DEX-INDUCED STRESS MICE<br />
Source : JOURNAL OF NEUROCHEMISTRY, Volume: 115, Supplement: 1, Pages: 78-78, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1536<br />
Author (s) : Tongmee, P., Punnakitidashem, P.<br />
Title : Impact <strong>of</strong> customer relationship management implementation on service operations management<br />
Source : <strong>2010</strong> 7th <strong>International</strong> Conference on Service Systems and Service Management, Proceedings <strong>of</strong> ICSSSM' 10, <strong>2010</strong>, Article<br />
number 5530195, Pages 462-467<br />
Document Type : Conference Paper<br />
Keywords : CRM; Customer relationship management; Operations management; Service encounter; Service operations<br />
<strong>Abstract</strong> : Customer Relationship Management (CRM) is one <strong>of</strong> key strategic tools for companies to enhance their relationship with<br />
customers. There is a significant impact <strong>of</strong> CRM implementation on customer services through improvement <strong>of</strong> customer<br />
service efficiency and effectiveness. Nevertheless, impact <strong>of</strong> CRM implementation on service operations management has not<br />
been addressed in literature. In this paper, the comprehensive review <strong>of</strong> marketing, sales, information technology and service<br />
operations management literature is conducted focusing on CRM implementation and service encounter. The conceptual<br />
model <strong>of</strong> adapted technology-facilitated service encounter is proposed from literature review and in depth study <strong>of</strong> two<br />
models which are service encounter triad and technology in service encounter model. The proposed model indicates that<br />
service culture, empowerment, control system, contact personnel selection, training and ethical climate are the service<br />
components impacted by CRM implementation. In additional, an analysis <strong>of</strong> CRM critical success factors is conducted<br />
together with the impacted components. The result indicates that service culture and contact personnel training are the two<br />
components which should be focused by service operations manager in order to ensure the success <strong>of</strong> CRM implementation<br />
and the fast return on CRM investment. Further discussion is noted to suggest additional components which should be<br />
considered as well as the future research study. ©<strong>2010</strong> IEEE.<br />
No.1537<br />
Author (s) : Tongrod, N., Kerdcharoen, T., Watthanawisuth, N., Tuantranont, A.<br />
Title : A low-cost data-glove for human computer interaction based on ink-jet printed sensors and ZigBee networks<br />
Source : Proceedings - <strong>International</strong> Symposium on Wearable Computers, ISWC, <strong>2010</strong>, Article number 5665850, 2p<br />
Document Type : Conference Paper<br />
Keywords : Conductive Polymer, Cost data, Data glove, Hand tracking, Ink-jet, PEDOT:PSS, Photo papers, Printed sensors, Telerobotics,<br />
ZigBee networks, ZigBee technology; Human computer interaction, Wearable computers; Sensors<br />
<strong>Abstract</strong> : In this paper, a data-glove based on new kind <strong>of</strong> sensors is presented as an alternative to expensive devices. These sensors<br />
were realized using a conductive polymer (PEDOT:PSS) thin film printed on glossy photo paper. To demonstrate the printed<br />
sensors, we constructed a data glove using such sensors and developed s<strong>of</strong>tware for real-time hand tracking. Wireless<br />
networks based on low-cost ZigBee technology were used to transfer data from the glove to a computer. This data-glove is<br />
very useful in many contexts such as telerobotics, rehabilitation and HCI applications.<br />
No.1538<br />
Author (s) : Toniti, P., Sirivisoot, S., Jandee, P., Srimontri, P., Puchadapirom, P., Doungchawee, G., Kasorndorkbua, C.<br />
Title : AE1/AE3, Vimentin and p63 immunolocalization in canine mammary gland tumours: Roles in differentiation between luminal<br />
epithelial and myoepithelial lineages<br />
Source : Asian Pacific Journal <strong>of</strong> Cancer Prevention, Volume 11, Issue 1, <strong>2010</strong>, Pages 227-230<br />
Document Type : Article<br />
Keywords : Canine mammary tumors; Luminal epithelial; Marker immunohistochemistry; Myoepithelial<br />
<strong>Abstract</strong> : Mammary tumors are by far the most common tumors in female dogs and effective treatment relies on prompt and accurate<br />
diagnostic procedures. Canine mammary tumors may originate from various cell types, such as luminal epithelial,<br />
558
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
myoepithelial and stromal cells. This study aimed to differentiate luminal epithelial and myoepithelial lineages, using specific<br />
markers including AE1/AE3, Vimentin, and p63. Such data can be useful for prognosis. Canine mammary tumors were<br />
collected by surgical resection and tissue samples were investigated using the avidin-biotin-immunoperoxidase method with<br />
used primary antibodies against AE1/AE3, vimentin, and p63. Luminal epithelial-origin tumors were found to be<br />
immunoreactive with AE1/AE3 and vimentin monoclonal antibody, while myoepithelial-origin tumors were positive for p63<br />
and vimentin. In addition, canine mixed tumors showed reactivity with all three antibodies. In summary, AE1/AE3, p63 and<br />
vimentin can be used as specific immunohistochemical markers to distinguish lumino-epithelial and myoepithelial lineages <strong>of</strong><br />
canine mammary tumors.<br />
No.1539<br />
Author (s) : Toomtong, P., Suksompong, S.<br />
Title : Intravenous fluids for abdominal aortic surgery.<br />
Source : Cochrane database <strong>of</strong> systematic reviews (Online), Issue 1, <strong>2010</strong>, Pages CD000991<br />
Document Type : Review<br />
Keywords : abdominal aorta, abdominal aorta aneurysm, arteriosclerosis, bleeding, colloid, fluid therapy, human, meta<br />
analysis, randomized controlled trial, review, solution and solubility; Aorta, Abdominal, Aortic Aneurysm,<br />
Abdominal, Arteriosclerosis, Blood Loss, Surgical, Colloids, Fluid Therapy, Humans, Randomized Controlled Trials as<br />
Topic, Solutions<br />
<strong>Abstract</strong> : BACKGROUND: Surgery on the abdominal aorta to treat aneurysms or occlusive disease is a major undertaking which requires<br />
intensive physiological support and fluid management. Blood products are <strong>of</strong>ten used but the main fluid replacement is with<br />
crystalloids or colloids. For years there has been controversy over which fluid is optimal and a number <strong>of</strong> studies have<br />
examined the subject. This is an update <strong>of</strong> a Cochrane review first published in 2000 and previously updated in 2002.<br />
OBJECTIVES: To determine the effectiveness <strong>of</strong> different non-blood replacement fluids used in abdominal aorta procedures<br />
with a view to identifying the optimal fluid for use. SEARCH STRATEGY: The Cochrane Peripheral Vascular Diseases Group<br />
searched their Specialised Register (August 2009) and the Cochrane Central Register <strong>of</strong> Controlled Trials (CENTRAL) (The<br />
Cochrane Library 2009, Issue 3) for publications describing randomised controlled trials <strong>of</strong> non-blood replacement fluids in<br />
abdominal aortic surgery. In addition, the reference lists from retrieved trials were screened for further information about<br />
trials. SELECTION CRITERIA: Randomised controlled trials assessing the effects <strong>of</strong> at least one specific non-blood fluid used for<br />
replacement therapy in operations on, and confined to, the abdominal aorta. DATA COLLECTION AND ANALYSIS: Data were<br />
extracted and then entered into the Review Manager s<strong>of</strong>tware where statistical analyses were performed. MAIN RESULTS:<br />
Thirty-eight trials involving 1589 patients were included. Patients undergoing aortic surgery had various physiological<br />
parameters measured before and after their operation (these were cardiac, respiratory, haematological, and biochemical).<br />
Patients were randomised to a fluid type. This review demonstrated that no single fluid affects any outcome measure<br />
significantly more than another fluid across a range <strong>of</strong> outcomes. The death rate in these studies was 2.45% (39 patients).<br />
AUTHORS' CONCLUSIONS: Despite the confirmed beneficial effects <strong>of</strong> colloids in this review, further studies are still required.<br />
There are no studies examining the effects <strong>of</strong> combination fluid therapy. The primary research outcome was death, for which<br />
results were limited; therefore, future studies should pay more attention to short-term outcomes such as minimising the<br />
need for allogenic blood transfusion, complications (organ failure), and length <strong>of</strong> stay in both the intensive care unit and<br />
hospital.<br />
No.1540<br />
Author (s) : Tosanguan, K.; Kapol, N.; Thavorncharoensap, M.; Suksomboon, N.<br />
Title : PERSPECTIVE OF THAI HEALTH-CARE PROFESSIONALS ON THAI HERBAL MEDICINE POLICY<br />
Source : VALUE IN HEALTH, Volume: 13, Issue: 7, Pages: A544-A544, Published: NOV <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1541<br />
Author (s) : Toskulkao, T., Pornchai, R., Akkarapatumwong, V., Vatanatunyakum, S., Govitrapong, P.<br />
Title : Alteration <strong>of</strong> lymphocyte opioid receptors in methadone maintenance subjects<br />
Source : Neurochemistry <strong>International</strong>, Volume 56, Issue 2, January <strong>2010</strong>, Pages 285-290<br />
559
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Document Type : Article<br />
Keywords : Heroin-abuse; Immune function; Lymphocyte; Methadone; Morphine; Naloxone; Opioid receptor<br />
<strong>Abstract</strong> : Methadone maintenance therapy is the most widely used treatment in patients with heroin addiction. Multiple studies have<br />
suggested that both current and former heroin addicts entering a methadone maintenance treatment program have altered<br />
immune function. Our previous study indicated that heroin addicts have depressed mitogen-stimulated lymphocyte<br />
proliferation and a decrease in the modulation <strong>of</strong> lymphocyte surface markers. This immunosuppression may be mediated via<br />
the direct interaction <strong>of</strong> opiates with lymphocyte opioid receptors. In order to test this hypothesis, the levels <strong>of</strong> opioid<br />
receptors on immune cells obtained from heroin users were determined using saturation binding, and it was found that<br />
former heroin addicts on methadone maintenance treatment had a significantly reduced maximum number (Bmax) <strong>of</strong><br />
[3H]naloxone binding. The Bmax values were 51.3±7.6fmol/mg protein for the non-addicted group and 25.3±3.1fmol/mg<br />
protein for the methadone maintenance group. Opioid receptor gene expression on the immune cell was determined using a<br />
semi-quantitative reverse-transcription polymerase chain reaction technique with specific pairs <strong>of</strong> primers to amplify mu- and<br />
delta-opioid receptor mRNAs. Both types <strong>of</strong> mRNAs were significantly decreased in lymphocytes obtained from the former<br />
heroin addicts on methadone maintenance subjects. Similarly, in an in vitro study, 100μM methadone significantly downregulated<br />
both mu- and delta-opioid receptor mRNA expressions in cultured lymphocytes obtained from naïve subjects. This<br />
effect was prevented by including 100μM naloxone or pretreating with 50ng/ml pertussis toxin. The data presented indicate<br />
that chronic opiate exposure was associated with down-regulation <strong>of</strong> G-protein-coupled opioid receptor gene expression in<br />
human lymphocytes. © 2009 Elsevier Ltd.<br />
No.1542<br />
Author (s) : Totanarungroj, K., Watcharaporn, C., Muangman, N.<br />
Title : Helpful CT findings for giving specific diagnosis <strong>of</strong> anterior mediastinal tumors<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 4, April <strong>2010</strong>, Pages 489-496<br />
Document Type : Review<br />
Keywords : Anterior mediastinal tumor; Computed tomography; Germ cell tumor; Lymphoma; Thymoma<br />
<strong>Abstract</strong> : Objective: To characterize the CT features <strong>of</strong> common anterior mediastinal tumors and evaluate CT findings that may help in<br />
suggesting specific diagnosis among these tumors. Material and Method: Fifty chest CT studies with pathological diagnosis <strong>of</strong><br />
thymoma (n = 28), mediastinal germ cell tumor (n = 14) and lymphoma (n = 8) were retrospectively reviewed by two<br />
radiologists who were blind to the pathological results. The CT findings <strong>of</strong> fat, cyst and calcification within the lesion, contrast<br />
enhancement, associated intrathoracic findings such as mediastinal invasion and lymph node enlargement were evaluated.<br />
Results: Fat density within the mass was present in 57.1% with germ cell tumor, which was significantly higher than other<br />
anterior mediastinal tumors (p < 0.05). The presence <strong>of</strong> associated mediastinal lymphadenopathy was significantly found in<br />
lymphoma (75.0%) compared to other tumors (p < 0.05). The other CT findings showed no significant difference among these<br />
diseases (p > 0.05). Conclusion: The CT findings that help in giving specific diagnosis <strong>of</strong> anterior mediastinal tumors which are<br />
fat attenuation and associated mediastinal lymphadenopathy. The presence <strong>of</strong> fat attenuation is highly suggestive <strong>of</strong> germ<br />
cell tumor and anterior mediastinal mass associated with mediastinal lymphadenopathy elsewhere is indicative <strong>of</strong> lymphoma.<br />
No.1543<br />
Author (s) : Traiperm, P., Boonkerd, T., Chantaranothai, P., Simpson, D.A.<br />
Title : Mnesithea thailandica a new species <strong>of</strong> Poaceae from Thailand<br />
Source : Kew Bulletin, Volume 65, Issue 2, <strong>2010</strong>, Pages 341-343<br />
Document Type : Article<br />
Keywords : Coelorachis; Mnesithea; Mnesithea thailandica; new species; Rottboelliinae; Thailand<br />
<strong>Abstract</strong> : Mnesithea thailandica, a new species <strong>of</strong> Poaceae subtribe Rottboelliinae from Thailand, is described and illustrated. © <strong>2010</strong><br />
The Board <strong>of</strong> Trustees <strong>of</strong> the Royal Botanic Gardens, Kew.<br />
No.1544<br />
Author (s) : Traiphol, R., Charoenthai, N., Srikhirin, T., Perahia, D.<br />
Title : Self-assembling into interconnected nanoribbons in thin films <strong>of</strong> hairy rod poly(9,9-di(2-ethylhexyl)fluorene): Effects <strong>of</strong><br />
concentration, substrate and solvent<br />
Source : Synthetic Metals, Volume 160, Issue 11-12, June <strong>2010</strong>, Pages 1318-1324<br />
Document Type : Article<br />
560
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : Aggregates; Conjugated polymer; Nanostructures; Photophysics; Self-organization<br />
<strong>Abstract</strong> : This study utilizes atomic force microscopy to investigate the self-assembling behaviors from dilute solution into thin film <strong>of</strong> a<br />
well-known conjugated polymer, poly(9,9-di(2-ethylhexyl)fluorene) (PF2/6). We have found that the structures <strong>of</strong> nanoscale<br />
aggregates depend on various experimental parameters including concentration, substrate and solvent. The self-assembling <strong>of</strong><br />
PF2/6 from 0.05 mg/mL solution in toluene onto SiOx/Si substrate results in the formation <strong>of</strong> interconnected nanoribbons<br />
with thickness and width <strong>of</strong> about 20 and 150 nm, respectively. Varying polymer concentration and type <strong>of</strong> substrate (SiOx/Si<br />
or mica) significantly affects the nanoscale structures. The change <strong>of</strong> solvent to chlorobenzene, a slightly more polar solvent<br />
with slower evaporation rate, causes the growth <strong>of</strong> ribbon width to micron size with slight increase <strong>of</strong> the thickness. When the<br />
solvents with higher evaporation rate (i.e. chlor<strong>of</strong>orm and dichloromethane) are used, densely packed nanoribbons are<br />
obtained. Its width also grows to micron size. The measurements <strong>of</strong> UV/vis absorption and photoluminescence spectra detect<br />
some discrepancies in pattern, reflecting the variation <strong>of</strong> local chain organization within thin films prepared by using different<br />
solvents. © <strong>2010</strong> Elsevier B.V. All rights reserved.<br />
No.1545<br />
Author (s) : Traiphol, R., Pattanatornchai, T., Srikhirin, T., Kerdcharoen, T., Osotchan, T.<br />
Title : Effects <strong>of</strong> steric anthracene moieties and keto defects on photophysics and color stability <strong>of</strong> poly(9,9-di(2-ethylhexyl)fluorenestat-anthracene)<br />
in different local environments<br />
Source : Macromolecular Research, Volume 18, Issue 12, December <strong>2010</strong>, Pages 1182-1190<br />
Document Type : Article<br />
Keywords : Aggregates; Conjugated polymers; Excimers; Optical materials; Photoluminescence spectroscopy; Self-assembling<br />
<strong>Abstract</strong> : UV/vis absorption and photoluminescence (PL) spectroscopy were used to examine the photophysical properties <strong>of</strong> statistical<br />
copolymers, poly(9,9-di(2-ethylhexyl)fluorene-stat-anthracene), with the mole ratio <strong>of</strong> the anthracene group ranging from 0 to<br />
20%. The incorporation <strong>of</strong> the anthracene group into the conjugated backbone <strong>of</strong> the polyfluorene affected the PL pattern<br />
and quantum yield <strong>of</strong> the isolated chain in a dilute solution. An investigation <strong>of</strong> the photophysical properties in solventnonsolvent<br />
system revealed the formation <strong>of</strong> low-energy non-emissive and emissive aggregates. The quantity <strong>of</strong> these<br />
aggregated species depends significantly on the mole ratio <strong>of</strong> anthracene group in the polymer backbone. The existence <strong>of</strong><br />
only 5 mol% <strong>of</strong> anthracene in the copolymer causes significant suppression <strong>of</strong> the well-known green emission (λ̃525 nm).<br />
However, a large quantity <strong>of</strong> non-emissive aggregates indicated by the red-shift peak at ̃415 nm in absorption spectra still<br />
formed. Increasing the mole ratio <strong>of</strong> the anthracene group to 20% suppressed both types <strong>of</strong> aggregates. Measurements <strong>of</strong> the<br />
absorption and PL spectra in the thin films showed consistent results. Although annealing <strong>of</strong> the homopolymer polyfluorene<br />
films at 150 °C in a vacuum oven caused a drastic increase in green emission, only a slight change was detected in the PL<br />
spectra <strong>of</strong> the copolymers. The presence <strong>of</strong> oxygen during annealing promoted the green emission <strong>of</strong> the polymer films.<br />
No.1546<br />
Author (s) : Traiphol, R., Potai, R., Charoenthai, N., Srikhirin, T., Kerdcharoen, T., Osotchan, T<br />
Title : Effects <strong>of</strong> Chain conformation and Chain length on degree <strong>of</strong> aggregation in assembled particles <strong>of</strong> conjugated polymer in<br />
solvents-nonsolvent: A spectroscopic study<br />
Source : Journal <strong>of</strong> Polymer Science, Part B: Polymer Physics, Volume 48, Issue 8, 15 April <strong>2010</strong>, Pages 894-904<br />
Document Type : Article<br />
Keywords : Aggregation; Colloids; Conjugated polymers; Photophysics; Self-assembly; Solvent effect<br />
<strong>Abstract</strong> : This article explores photophysical properties and aggregation behaviors <strong>of</strong> conjugated polymer, poly[2-methoxy, 5-(2'ethylhexyloxy)-1,4-phenylenevinylene]<br />
(MEH-PPV), in various solvent-nonsolvent systems by utilizing UV/vis absorption and<br />
photoluminescence (PL) spectroscopy. The isolated chains <strong>of</strong> MEH-PPV dispersed in solvents including dichloromethane,<br />
chlor<strong>of</strong>orm, and tetrahydr<strong>of</strong>uran adopt either extended or collapsed conformations depending on local polymer-solvent<br />
interactions. Aggregation <strong>of</strong> the MEH-PPV in these solvents is induced by addition <strong>of</strong> a poor solvent, cyclohexane. The<br />
formation <strong>of</strong> aggregates is indicated by the appearance <strong>of</strong> distinct red-shift peaks in the absorption and PL spectra. The<br />
degree <strong>of</strong> aggregation in each solvent-nonsolvent system is compared by means <strong>of</strong> absorbance and PL intensity <strong>of</strong> the<br />
aggregate bands. In early stage <strong>of</strong> the aggregation, the amount <strong>of</strong> aggregates in system is controlled by the solubility <strong>of</strong><br />
polymer. When the polymer chains are forced to densely pack within assembled particles by increasing ratio <strong>of</strong> cyclohexane<br />
to 99 v/v %, the conformation <strong>of</strong> individual chain plays important role. We have found that the extended chains facilitate the<br />
aggregation in the assembled particles. Increasing chain length <strong>of</strong> polymer promotes the aggregation in early stage and<br />
561
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
densely packed particles. Size distribution <strong>of</strong> the assembled particles is also found to depend on the choice <strong>of</strong> solvent. ©<br />
<strong>2010</strong> Wiley Periodicals, Inc.<br />
No.1547<br />
Author (s) : Trakarnsanga, A., Sriprayoon, T., Akaraviputh, T., Tongdee, T.<br />
Title : Massive hemobilia from a ruptured hepatic artery aneurysm detected by endoscopic ultrasound (EUS) and successfully<br />
treated<br />
Source : Endoscopy, Volume 42, Issue SUPPL. 2, <strong>2010</strong>, Pages E340-E341<br />
Document Type : Note<br />
Keywords : enbucrilate; aneurysm, artificial embolism, case report, diagnostic test accuracy study, endoscopic echography, endoscopic<br />
retrograde cholangiopancreatography, hemobilia, hepatic artery, human, jaundice, liver artery aneurysm, liver<br />
disease, male, note, nuclear magnetic resonance imaging, priority journal, radiodiagnosis<br />
<strong>Abstract</strong> : –<br />
No.1548<br />
Author (s) : Trakarnvanich, T., Chalapipat, O., Disthabanchong, S., Kurathong, S., Praditpornsilpa, K., Stitchantrakul, W., Chailurkit, L.-O.<br />
Title : Effect <strong>of</strong> high dose ergocalciferol in chronic kidney disease patients with 25-hydroxyvitamin D deficiency<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 8, August <strong>2010</strong>, Pages 885-891<br />
Document Type : Article<br />
Keywords : 25-hydroxyvitamin D; Ergocalciferol; Hyperparathyroidism; PTH; Vitamin D<br />
<strong>Abstract</strong> : Objective: To evaluate 25 hydroxyvitamin D (25-OH-D) deficiency in a cohort <strong>of</strong> predialysis CKD patients and the treatment<br />
effect and safety <strong>of</strong> high dose ergocalciferol supplement in predialysis CKD. Material and Method: Fifty-six predialysis CKD<br />
patients who came for a regular visit at a single hospital with calculated glomerular filtration rate ≤ 60 mL/min/1.73 m2 were<br />
screened for 25-OH-D levels. Forty-four patients with 25-OH-D deficiency were recruited into this prospective observational<br />
study that examined the effect <strong>of</strong> high dose oral ergocalciferol supplementation. After eight weeks, 37 patients completed<br />
the follow-up and biochemical parameters were reevaluated and analyzed. Results: The mean 25-OH-D level <strong>of</strong> 56 patients<br />
was 25.6 ± 8 ng/mL. Forty-four (78.5%) patients had 25-OH-D levels less than 30 ng/mL and four (7.1%) had severe deficiency<br />
with the level less than 15 ng/mL. High dose ergocalciferol upplement successively increased 25-OH-D levels in 35 (95%)<br />
patients. 25-OH-D levels increased significantly from 22 ± 4.8 to 34.5 ± 10.8 ng/mL after eight weeks (p < 0.001). During the<br />
study period, there were no changes in serum calcium, phosphate, and PTH. There was no other side effect associated with<br />
the treatment. Conclusion: 25-OH-D deficiency were found in this cohort <strong>of</strong> predialysis CKD patients. Ergocalciferol was a safe<br />
and effective supplement for the 25-OH-D in predialysis CKD.<br />
No.1549<br />
Author (s) : Treegate, K., Rasamessard, A., Osotchan, T., Hodak, J.H.<br />
Title : X-Y sample scanning stage and calibration method suitable for single-molecule detection<br />
Source : Sensors and Actuators, B: Chemical, Volume 150, Issue 1, 21 October <strong>2010</strong>, Pages 239-246<br />
Document Type : Article<br />
Keywords : Fluorescence spectroscopy; Optical detection; Scanning confocal microscopy; Single molecule spectroscopy<br />
<strong>Abstract</strong> : This paper describes the construction <strong>of</strong> a positioning device for sample scanning in the x and y directions suitable for single<br />
molecule fluorescence experiments. The mechanism uses a simple parallelogram flexure cut out <strong>of</strong> a single aluminum plate<br />
and two amplified piezoelectric actuators <strong>of</strong> the type used for microscope objective focus adjustment. A displacement range<br />
<strong>of</strong> 75 μm on each axis is obtained. The stage can be used to implement a sample scanning confocal microscope for single<br />
molecule spectroscopy applications using either inverted or up-right microscopes. Images with diffraction limited resolution<br />
can be obtained with this scanning stage. This is demonstrated by imaging glass beads labeled with the DY475 fluorescent<br />
dye and single rhodamine molecules. Micron sized range images <strong>of</strong> 256 × 256 pixels can be obtained with dwell times down<br />
to 0.5 ms/pixel. A novel direct calibration in which the mechanical response obtained from the line pr<strong>of</strong>iles for forward and<br />
reverse motion is used to account for the hysteresis <strong>of</strong> the stage. The target molecules are then located within the focus <strong>of</strong><br />
the laser beam by using its corrected position. The performance <strong>of</strong> this scanning device and correction technique are<br />
demonstrated for the acquisition <strong>of</strong> fluorescence trajectories <strong>of</strong> individual rhodamine molecules. © <strong>2010</strong> Published by<br />
Elsevier B.V.<br />
562
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.1550<br />
Author (s) : Treekitkarnmongkol, W., Suthiphongchai, T.<br />
Title : High expression <strong>of</strong> ErbB2 contributes to cholangiocarcinoma cell invasion and proliferation through AKT/p70S6K<br />
Source : World Journal <strong>of</strong> Gastroenterology, Volume 16, Issue 32, 28 August <strong>2010</strong>, Pages 4047-4054<br />
Document Type : Article<br />
Keywords : Akt; Cell proliferation; Cholangiocarcinoma; Erbb2; Invasion; P70s6k<br />
<strong>Abstract</strong> : AIM: To compare the impact <strong>of</strong> ErbB2 on cell invasion and proliferation in cholangiocarcinoma (CCA) cell lines. METHODS:<br />
Level <strong>of</strong> endogenous ErbB2 expression in three CCA cell lines, namely HuCCA-1, KKU-100 and KKU-M213, was determined by<br />
real-time reversetranscriptase polymerase chain reaction. Two ErbB2 inhibitory methods, a small molecule ErbB2 kinase<br />
inhibitor (AG825) and siRNA, were used to disrupt ErbB2 function in the cell lines. CCA cell invasion, motility and proliferation<br />
under ErbB2-disrupted conditions were detected using Transwell and 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium<br />
bromide assays. In addition, ErbB2 downstream effectors were investigated by Western blotting analysis. RESULTS:<br />
Suppression <strong>of</strong> ErbB2 activity, using a specific kinase inhibitor (AG825), reduced invasion, motility and proliferation <strong>of</strong> all three<br />
CCA cell lines. The ability <strong>of</strong> this drug to inhibit neoplastic properties (invasion, motility and proliferation) increased<br />
concomitantly with the level <strong>of</strong> ErbB2 expression. Similarly, knockdown <strong>of</strong> ErbB2 level by siRNA inhibited cell invasion and<br />
proliferation <strong>of</strong> KKU-M213, a high-ErbB2-expressing cell, better than those <strong>of</strong> the lower-ErbB2-expressing cells, HuCCA-1 and<br />
KKU-100. Thus, both inhibitory methods indicated that there is more ErbB2-dependency for malignancy <strong>of</strong> the high-ErbB2expressing<br />
cell, KKU-M213, than for that <strong>of</strong> low-ErbB2-expressing ones. In addition, interrupting ErbB2 activity decreased<br />
phosphorylation <strong>of</strong> AKT and p70S6K, but not extracellular signal-regulated kinase 1/2, in the high-ErbB2-expressing CCA cell<br />
line. CONCLUSION: Our data indicated that high ErbB2 expression enhances CCA invasion, motility and proliferation via the<br />
AKT/p70S6K pathway, which suggests the possibility <strong>of</strong> targeting these molecules for CCA therapy © <strong>2010</strong> Baishideng.<br />
No.1551<br />
Author (s) : Treepongkaruna, S., Pienvichit, P., Phuapradit, P., Kodcharin, P., Wattanasirichaigoon, D.<br />
Title : Mutations <strong>of</strong> ATP7B gene in two Thai siblings with Wilson disease<br />
Source : Asian Biomedicine, Volume 4, Issue 1, February <strong>2010</strong>, Pages 163-169<br />
Document Type : Article<br />
Keywords : ATP7B gene; Cirrhosis; Liver disease; Wilson disease<br />
<strong>Abstract</strong> : Background: Wilson disease (WD) is an autosomal recessive disorder <strong>of</strong> copper metabolism caused by mutations in ATP7B<br />
gene. Objective: Report the clinical data and mutation analysis <strong>of</strong> two Thai siblings suspected <strong>of</strong> WD. Subject and methods: A<br />
13-year-old boy who presented with cirrhosis, arthralgia, hypoalbuminemia, and coagulopathy, and his 11-year-old sister who<br />
was asymptomatic but had hepatomegaly with elevation <strong>of</strong> transaminases, were studied. Mutation analysis <strong>of</strong> ATP7B gene<br />
and mRNA analysis was performed in both patients and their parents. Results: Investigations were consistent with WD, and<br />
their liver diseases improved after standard treatment for WD. DNA analyses in these two patients revealed two novel<br />
mutations, which were a deletion <strong>of</strong> the first 2bp <strong>of</strong> exon 6 (c.1870-1871delGA), and a single base substitution from A to G at<br />
nucleotide 4075 (c.4075A>G) in the exon 20 (p.M1359V). PCR-restriction digestion with NcoI restriction enzyme was employed<br />
as the second method for confirmation <strong>of</strong> the c.4075A>G mutation and for rapid screening in 100 chromosomes from<br />
unrelated healthy controls, and this variant was not present in the controls. The c.1870-1871delGA deletion caused a<br />
frameshift effect, which results in a premature stop codon (p.E624fsX753), and the p.M1359V mutation is a substitution <strong>of</strong><br />
methionine with valine, which may have effects upon its orientation and interaction with other adjacent amino acids.<br />
Conclusion: Two novel mutations <strong>of</strong> ATP7B gene were identified in two Thai siblings with WD.<br />
No.1552<br />
Author (s) : Treeprasertsuk, S., Silachamroon, U., Krudsood, S., Huntrup, A., Suwannakudt, P., Vannaphan, S., Wilairatana, P.<br />
Title : Ursodeoxycholic acid and artesunate in the treatment <strong>of</strong> severe falciparum malaria patients with jaundice<br />
Source : Journal <strong>of</strong> Gastroenterology and Hepatology, Volume 25, Issue 2, February <strong>2010</strong>, Pages 362-368<br />
Document Type : Article<br />
Keywords : Artesunate; Falciparum malaria; Jaundice; Ursodeoxycholic acid<br />
<strong>Abstract</strong> : Background and Aims: Plasmodium falciparum (PF) infection can lead to severe complications. Ursodeoxycholic acid (UDCA) is<br />
increasingly used for the treatment <strong>of</strong> cholestatic liver diseases. The present study aims to determine the effects <strong>of</strong><br />
combined UDCA and artesunate compared to placebo and artesunate on the improvement <strong>of</strong> liver tests in severe PF<br />
jaundiced patients. Methods: All severe PF jaundiced patients, aged ≥ 15 years and diagnosed as having severe malaria<br />
563
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
according to WHO 2000 criteria, were enrolled. Patients with evidence <strong>of</strong> biliary obstruction, other cholestatic liver diseases<br />
and those who were pregnant were excluded. Patients were randomized to receive either oral UDCA or placebo for 2 weeks<br />
in additional to artesunate. All patients were admitted for at least 14 days to monitor the result <strong>of</strong> the treatment. Results:<br />
Seventy-four severe PF malaria patients with jaundice were enrolled. Both groups had similar demographic and laboratory<br />
tests, with the exception being more males in the UDCA group than in the placebo group (P = 0.04). The median <strong>of</strong><br />
percentage change <strong>of</strong> total bilirubin and aminotransferase levels at the end <strong>of</strong> weeks 1, 2, 3 and 4 showed no difference<br />
between the two groups. Only the median <strong>of</strong> percentage change <strong>of</strong> alkaline phosphatase at the end <strong>of</strong> week one compared<br />
with the baseline values showed less increment in the UDCA group than in the placebo group (P = 0.04). No serious adverse<br />
events were seen during the 4 weeks <strong>of</strong> follow up. Conclusions: In severe PF malaria patients with jaundice, combined<br />
therapy with UDCA and artesunate is safe, but does not significantly improve liver tests compared to placebo and artesunate.<br />
© <strong>2010</strong> Journal <strong>of</strong> Gastroenterology and Hepatology Foundation and Blackwell Publishing Asia Pty Ltd.<br />
No.1553<br />
Author (s) : Tresilwised, N., Pithayanukul, P., Mykhaylyk, O., Holm, P.S., Holzmüller, R., Anton, M., Thalhammer, S., Adigüzel, D., Döblinger,<br />
M., Plank, C.<br />
Title : Boosting oncolytic adenovirus potency with magnetic nanoparticles and magnetic force<br />
Source : Molecular Pharmaceutics, Volume 7, Issue 4, 2 August <strong>2010</strong>, Pages 1069-1089<br />
Document Type : Article<br />
Keywords : magnetic drug targeting; magnetic nanoparticles; magnet<strong>of</strong>ection; Oncolytic adenovirus<br />
<strong>Abstract</strong> : Oncolytic adenoviruses rank among the most promising innovative agents in cancer therapy. We examined the potential <strong>of</strong><br />
boosting the efficacy <strong>of</strong> the oncolytic adenovirus dl520 by associating it with magnetic nanoparticles and magnetic-fieldguided<br />
infection in multidrug-resistant (MDR) cancer cells in vitro and upon intratumoral injection in vivo. The virus was<br />
complexed by self-assembly with core-'shell nanoparticles having a magnetite core <strong>of</strong> about 10 nm and stabilized by a shell<br />
containing 68 mass % lithium 3-[2-(perfluoroalkyl) ethylthio]propionate) and 32 mass % 25 kDa branched polyethylenimine.<br />
Optimized virus binding, sufficiently stable in 50% fetal calf serum, was found at nanoparticle-to-virus ratios <strong>of</strong> 5 fg <strong>of</strong> Fe per<br />
physical virus particle (VP) and above. As estimated from magnetophoretic mobility measurements, 3,600 to 4,500 magnetite<br />
nanocrystallites were associated per virus particle. Ultrastructural analysis by electron and atomic force microscopy showed<br />
structurally intact viruses surrounded by magnetic particles that occasionally bridged several virus particles. Viral uptake into<br />
cells at a given virus dose was enhanced 10-fold compared to nonmagnetic virus when infections were carried out under the<br />
influence <strong>of</strong> a magnetic field. Increased virus internalization resulted in a 10-fold enhancement <strong>of</strong> the oncolytic potency in<br />
terms <strong>of</strong> the dose required for killing 50% <strong>of</strong> the target cells (IC50 value) and an enhancement <strong>of</strong> 4 orders <strong>of</strong> magnitude in<br />
virus progeny formation at equal input virus doses compared to nonmagnetic viruses. Furthermore, the full oncolytic effect<br />
developed within two days postinfection compared with six days in a nonmagnetic virus as a reference. Plotting target cell<br />
viability versus internalized virus particles for magnetic and nonmagnetic virus showed that the inherent oncolytic productivity<br />
<strong>of</strong> the virus remained unchanged upon association with magnetic nanoparticles. Hence, we conclude that the mechanism <strong>of</strong><br />
boosting the oncolytic effect by magnetic force is mainly due to the improved internalization <strong>of</strong> magnetic virus complexes<br />
resulting in potentiated virus progeny formation. Upon intratumoral injection and application <strong>of</strong> a gradient magnetic field in a<br />
murine xenograft model, magnetic virus complexes exhibited a stronger oncolytic effect than adenovirus alone. We propose<br />
that this approach would be useful during in vivo administration to tumor-feeding blood vessels to boost the efficacy <strong>of</strong> the<br />
primary infection cycle within the tumor. For systemic application, further modification <strong>of</strong> magnetic adenovirus complexes for<br />
shielding and retargeting <strong>of</strong> the whole magnetic virus complex entity is needed. © <strong>2010</strong> American Chemical Society.<br />
No.1554<br />
Author (s) : Tresukosol, D., Pornratanarangsi, S., Chotinaiwattarakul, C., Tungsubutra, W., Phankingthongkum, R., Wongpraparut, N., Lirdwilai,<br />
T., Dapang, T., Panchavinnin, P.<br />
Title : Evaluation <strong>of</strong> the firebird sirololimus eluting stent in all comers with coronary artery stenosis.<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand = Chotmaihet thangphaet, Volume 93 Suppl 1, January <strong>2010</strong>, Pages S11-20<br />
Document Type : Article<br />
Keywords : immunosuppressive agent, rapamycin; aged, angiocardiography, article, coronary artery disease, coronary artery<br />
obstruction, drug eluting stent, evaluation, female, follow up, human, male, methodology, middle aged, prospective<br />
study, radiography, register, survival, Thailand, transluminal coronary angioplasty, treatment outcome; Aged, Aged, 80 and<br />
over, Angioplasty, Transluminal, Percutaneous Coronary, Coronary Angiography, Coronary Artery Disease, Coronary<br />
564
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Stenosis, Drug-Eluting Stents, Female, Follow-Up Studies, Humans, Immunosuppressive Agents, Male, Middle<br />
Aged, Prospective Studies, Registries, Sirolimus, Survival, Thailand, Treatment Outcome<br />
<strong>Abstract</strong> : OBJECTIVE: Percutaneous coronary intervention (PCI) has been widely used to treat obstructive coronary artery disease. With<br />
the advent <strong>of</strong> drug-eluting stent (DES) in real world registry was proved as promising therapy. The limitation <strong>of</strong> the use <strong>of</strong> DES<br />
is the limited health care expenditure. We propose the use <strong>of</strong> Chinese made DES among Thai patients and that this will solve<br />
the cost issue. The clinical result <strong>of</strong> this DES has not been well known. METHODS: Prospective study from November 2005 to<br />
March 2007 using the structured registry form to evaluate the safety and efficacy <strong>of</strong> new Chinese made Firebird sirolimus<br />
eluting stent (Firebird SES) on clinical parameters from in-hospital, 30 days and 12 months or longer term follow-up. End<br />
point is major adverse cardiac event (MACE) including death, MI, TLR and CABG at 30 day and cumulative MACE at 12 month<br />
follow-up. RESULTS: Ninety consecutive patients who were treated with Firebird stent implantation (107 target lesions) were<br />
analyzed. Angiographic success (defined as < 30% diameter stenosis) was 85%. Procedure success was 77.8%. MACE at 30 day<br />
was 16.6%, cumulative MACE at 12 months was 18.8%. There were total 9 deaths during the study period, two deaths<br />
occurred at before 30 days, 3 deaths occurred before 12 months and other 4 deaths occurred after 12 months to 1305 days.<br />
Eighty patients (88.9%) had either <strong>of</strong>fice visit or telephone call follow-up after 12 months, 38 patients (42.2%) underwent<br />
clinical driven coronary arteriography, binary restenosis was 26.3%. Shock and smoking history was the analyzed predictor <strong>of</strong><br />
MACE at follow-up. CONCLUSION: The implantation <strong>of</strong> Firebird DES in unselected patients (all comers) is safe, effective and<br />
could be an alternative choice <strong>of</strong> stent for Thai patients.<br />
No.1555<br />
Author (s) : Tresukosol, D., Sudjaritruk, S., Pornratanarangsi, S., Chotinaiwattarakul, C., Phankingthongkum, R., Tungsubutra, W.,<br />
Wongpraparat, N., Lerdwilai, T., Panchavinnin, P.<br />
Title : Early and intermediate outcomes <strong>of</strong> left main coronary intervention.<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand = Chotmaihet thangphaet, Volume 93 Suppl 1, January <strong>2010</strong>, Pages S21-28<br />
Document Type : Article<br />
Keywords : acetylsalicylic acid, antithrombocytic agent, clopidogrel, drug derivative, ticlopidine; aged, angiocardiography, article, coronary<br />
artery disease, coronary blood vessel, echography, female, follow up, heart left ventricle<br />
function, human, male, methodology, middle aged, radiography, retrospective study, stent, survival, time, transluminal<br />
coronary angioplasty, treatment outcome; Aged, Angioplasty, Transluminal, Percutaneous Coronary, Aspirin, Coronary<br />
Angiography, Coronary Disease, Coronary Vessels, Female, Follow-Up Studies, Humans, Male, Middle Aged, Platelet<br />
Aggregation Inhibitors, Retrospective Studies, Stents, Survival, Ticlopidine, Time Factors, Treatment Outcome, Ventricular<br />
Function, Left<br />
<strong>Abstract</strong> : OBJECTIVE: We examined the immediate and long-term outcomes after stenting <strong>of</strong> all comers for left main coronary artery<br />
(LMCA) stenoses. BACKGROUND: Left main coronary artery disease is regarded as an absolute contraindication for coronary<br />
angioplasty. Recently, several reports on protected or unprotected LMCA stenting, or both, suggested the possibility <strong>of</strong><br />
percutaneous intervention for this prohibited area. MATERIAL AND METHOD: Eighty-one consecutive patients with LMCA<br />
stenoses were treated with stents. The post-stent antithrombotic regimens were aspirin and clopidogrel. The major adverse<br />
cardiac events (MACE) including death, Q-wave myocardial infarction, or repeat target lesion revascularization were followed.<br />
Patients were followed very closely and all attended <strong>of</strong>fice visit at 12 months. RESULTS: The procedural success rate was<br />
86.4%, with no episodes <strong>of</strong> acute thrombosis. Follow-up angiography was performed in 30 <strong>of</strong> 65 eligible patients (46.2%).<br />
Angiographic restenosis occurred in eight patients (9.9%). Cumulative death occurred in 16 patients (19.7%). MACE at 30 day<br />
and 12-month was 12.3% and 33.3% respectively. From multivariate analysis, dialysis (HR =3.22, p = 0.048), urgent PCI (HR<br />
=2.39, p = 0.036), post-procedure TIMI flow < 3 (HR =25.99, p = 0.001) and final kissing balloon inflation (HR = 0.30, p = 0.04)<br />
were independent predictors <strong>of</strong> MACE at 12-month. There was one definite late stent thrombosis (1.2%). CONCLUSION:<br />
Stenting <strong>of</strong> LMCA stenosis may be a safe and effective alternative to CABG in carefully selected patients. Further studies in<br />
larger patient populations are needed to assess late outcome.<br />
No.1556<br />
Author (s) : Tribuddharat, C., Polwichai, P., Champreeda, P., Srifuengfung, S.<br />
Title : The sequence <strong>of</strong> pbp2b from penicillin-resistant Streptococcus pneumoniae in Thailand.<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand = Chotmaihet thangphaet, Volume 93 Suppl 5, November <strong>2010</strong>, Pages S16-26<br />
Document Type : Article<br />
565
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : aminoacyltransferase, antiinfective agent, bacterial protein, cefotaxime, penicillin binding protein, penicillin binding protein 2b,<br />
Streptococcus, penicillin derivative, penicillin-binding protein 2b, Streptococcus; amino acid sequence, article, bacterial<br />
gene, drug effect, gene amplification, genetics, genotype, human, isolation and purification, microbiological<br />
examination, molecular genetics, mutation, nucleotide sequence, penicillin resistance, polymerase chain<br />
reaction, Streptococcus pneumoniae, Thailand; Amino Acid Sequence, Aminoacyltransferases, Anti-Bacterial Agents, Bacterial<br />
Proteins, Base Sequence, Cefotaxime, Gene Amplification, Genes, Bacterial, Genotype, Humans, Microbial Sensitivity<br />
Tests, Molecular Sequence Data, Mutation, Penicillin Resistance, Penicillin-Binding Proteins, Penicillins, Polymerase Chain<br />
Reaction, Streptococcus pneumoniae, Thailand<br />
<strong>Abstract</strong> : Penicillin resistance in Streptococcus pneumoniae isolates results from altered penicillin-binding proteins (PBPs), especially<br />
PBP2, which has a reduced affinity to penicillin. This study evaluated drug resistance and the gene sequence <strong>of</strong> the conserved<br />
motif pbp2b <strong>of</strong> penicillin-resistant isolates in Thailand. Penicillin-resistant pneumococci with minimum inhibitory<br />
concentrations (MIC) for penicillin > or = microg/ ml and penicillin-susceptible strains were identified from clinical specimens.<br />
The pbp2b genes were amplified by polymerase chain reaction (PCR), and the purified PCR product was cloned into E. coli.<br />
The recombinant plasmid clones containing pbp2b were sequenced and evaluated for mutations corresponding to penicillin<br />
and cefotaxime resistance. Penicillin-susceptible S. pneumoniae isolates were susceptible to 12 other antibiotics tested (range<br />
95-100%) while penicillin-nonsusceptible isolates were resistant to most antibiotics except amoxicillin/clavulanate and<br />
lev<strong>of</strong>loxacin. Sequence analysis <strong>of</strong> pbp2b showed a substitution <strong>of</strong> A for T451 next to the region <strong>of</strong> the SSN triad in all six<br />
resistant isolates tested and mutations clustered around the KTG triad in two isolates. Using the ClustalW alignment program,<br />
Thai isolates differed from those <strong>of</strong> European countries, but were more similar to those from Japan than Korea. Penicillin or<br />
cefotaxime resistance in S. pneumoniae in Thailand was due to affinity reduction <strong>of</strong> PBP2b, similar to changes found in other<br />
Asian isolates.<br />
No.1557<br />
Author (s) : Tricou, V., Minh, N.N., van, T.P., Lee, S.J., Farrar, J., Wills, B., Tran, H.T., Simmons, C.P.<br />
Title : A randomized controlled trial <strong>of</strong> chloroquine for the treatment <strong>of</strong> dengue in vietnamese adults<br />
Source : PLoS Neglected Tropical Diseases, Volume 4, Issue 8, August <strong>2010</strong>, Article number e785<br />
Document Type : Article<br />
Keywords : chloroquine, interleukin 10, interleukin 12p70, interleukin 1beta, interleukin 6, interleukin 8, nonstructural protein<br />
1, placebo, tumor necrosis factor alpha, antivirus agent, virus antigen; adult, anorexia, article, blood transfusion, CD4+ T<br />
lymphocyte, CD8+ T lymphocyte, clinical trial, controlled clinical trial, controlled study, cytokine release, dengue, Dengue<br />
virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4, double blind procedure, drug efficacy, female, fever, fluid<br />
therapy, hematemesis, human, incidence, major clinical study, male, randomized controlled trial, T lymphocyte<br />
activation, thrombocyte count, Viet Nam, viremia, vomiting, adolescent, blood, pathology, time, virology, virus load;<br />
Adolescent, Adult, Antigens, Viral, Antiviral Agents, Chloroquine, Dengue, Double-Blind<br />
Method, Female, Humans, Male, Placebos, Time Factors, Vietnam, Viral Load, Viremia, Young Adult<br />
<strong>Abstract</strong> : Background:There is currently no licensed antiviral drug for treatment <strong>of</strong> dengue. Chloroquine (CQ) inhibits the replication <strong>of</strong><br />
dengue virus (DENV) in vitro. Methods and Findings:A double-blind, randomized, placebo-controlled trial <strong>of</strong> CQ in 307 adults<br />
hospitalized for suspected DENV infection was conducted at the Hospital for Tropical Diseases (Ho Chi Minh City, Vietnam)<br />
between May 2007 and July 2008. Patients with illness histories <strong>of</strong> 72 hours or less were randomized to a 3-day course <strong>of</strong> CQ<br />
(n = 153) or placebo (n = 154). Laboratory-confirmation <strong>of</strong> DENV infection was made in 257 (84%) patients. The primary<br />
endpoints were time to resolution <strong>of</strong> DENV viraemia and time to resolution <strong>of</strong> DENV NS1 antigenaemia. In patients treated<br />
with CQ there was a trend toward a longer duration <strong>of</strong> DENV viraemia (hazard ratio (HR) = 0.80, 95% CI 0.62?1.05), but we did<br />
not find any difference for the time to resolution <strong>of</strong> NS1 antigenaemia (HR = 1.07, 95% CI 0.76?1.51). Interestingly, CQ was<br />
associated with a significant reduction in fever clearance time in the intention-to-treat population (HR = 1.37, 95% CI<br />
1.08?1.74) but not in the per-protocol population. There was also a trend towards a lower incidence <strong>of</strong> dengue hemorrhagic<br />
fever (odds ratio = 0.60, PP 95% CI 0.34-1.04) in patients treated with CQ. Differences in levels <strong>of</strong> T cell activation or pro- or<br />
antiinflammatory plasma cytokine concentrations between CQ- and placebo-treated patients did not explain the trend<br />
towards less dengue hemorrhagic fever in the CQ arm. CQ was associated with significantly more adverse events, primarily<br />
vomiting. Conclusions:CQ does not reduce the durations <strong>of</strong> viraemia and NS1 antigenaemia in dengue patients. Further trials,<br />
with appropriate endpoints, would be required to determine if CQ treatment has any clinical benefit in dengue. © <strong>2010</strong><br />
Tricou et al.<br />
566
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.1558<br />
Author (s) : Tritilanunt, S.<br />
Title : Performance evaluation <strong>of</strong> non-parallelizable client puzzles for defeating DoS attacks in authentication protocols<br />
Source : Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in<br />
Bioinformatics), Volume 6166 LNCS, <strong>2010</strong>, Pages 358-365<br />
Document Type : Conference Paper<br />
Keywords : Authentication protocols, Client puzzles, DoS attacks, Performance analysis, Performance evaluation;<br />
Authentication, Computer crime; Data privacy<br />
<strong>Abstract</strong> : We provides an evaluation <strong>of</strong> non-parallelizable puzzles used to prevent DoS in authentication protocols. With an evaluation<br />
based on a simulation and performance analysis, this approach helps a responder to resist against DoS, as well as improves<br />
the throughput <strong>of</strong> services for legitimate clients. Another key strength is that the construction and verification at the<br />
responder is simple and fast. © <strong>2010</strong> Springer-Verlag Berlin Heidelberg.<br />
No.1559<br />
Author (s) : Tritilanunt, S., Sivakorn, S., Juengjincharoen, C., Siripornpisan, A.<br />
Title : Entropy-based input-output traffic mode detection scheme for DoS/DDoS attacks<br />
Source : ISCIT <strong>2010</strong> - <strong>2010</strong> 10th <strong>International</strong> Symposium on Communications and Information Technologies, <strong>2010</strong>, Article number<br />
5665097, Pages 804-809<br />
Document Type : Conference Paper<br />
Keywords : DoS/DDoS attacks; Entropy-based detection<br />
<strong>Abstract</strong> : Denial-<strong>of</strong>-service attacks (DoS) and distributed denial-<strong>of</strong>-service attacks (DDoS) attempt to temporarily disrupt users or<br />
computer resources to cause service unavailability to legitimate users in the internetworking system. The most common type<br />
<strong>of</strong> DoS attack occurs when adversaries flood a large amount <strong>of</strong> bogus data to interfere or disrupt the service on the server. By<br />
using a volume-based scheme to detect such attacks, this technique would not be able to inspect short-term denial-<strong>of</strong>service<br />
attacks, as well as cannot distinguish between heavy load from legitimate users and huge number <strong>of</strong> bogus messages<br />
from attackers. As a result, this paper provides a detection mechanism based on a technique <strong>of</strong> entropy-based input-output<br />
traffic mode detection scheme. The experimental results demonstrate that our approach is able to detect several kinds <strong>of</strong><br />
denial-<strong>of</strong>-service attacks, even small spike <strong>of</strong> such attacks. ©<strong>2010</strong> IEEE.<br />
No.1560<br />
Author (s) : Tu, H.A.T.; Riewpaiboon, A.; Woerdenbag, H.J.; Postma, M.J.; Li, S.C.<br />
Title : COST-OF-ILLNESS OF CHRONIC HEPATITIS B INFECTION IN VIETNAM<br />
Source : VALUE IN HEALTH, Volume: 13, Issue: 7, Pages: A548-A548, Published: NOV <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1561<br />
Author (s) : Tuchinda, P., Gaspari, A.A.<br />
Title : Langerhans cells in allergic contact dermatitis<br />
Source : Giornale Italiano di Dermatologia e Venereologia, Volume 145, Issue 6, December <strong>2010</strong>, Pages 747-762<br />
Document Type : Review<br />
Keywords : Antigen presentation; Dermatitis, allergic contact; Immune modulation; Langerhans cells<br />
<strong>Abstract</strong> : Allergic contact dermatitis (ACD) is a common skin disease that has significant socio-economic impact ACD is mediated by a Tcell<br />
mediated inflammatory reaction. Langerhans cells (LCs) are an epidermal DCs subset specialized in antigen presentation.<br />
After hapten exposure, LCs play a major role as in induction adaptive immune response against allergens. LCs recognize, take<br />
up and process haptens and migrate to the local draining lymph nodes. However, LCs specific functions and the LCs<br />
migration to local draining lymph nodes are not yet clearly defined. Recent advance in the knowledge <strong>of</strong> LCs function has<br />
increased in the past decades including the evidence for a tolerogenic function <strong>of</strong> LCs. The present review will focus on the<br />
role for LCs response to contact allergens.<br />
567
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.1562<br />
Author (s) : Tummaruk, P., Kesdangsakonwut, S., Prapasarakul, N., Kaeoket, K.<br />
Title : Endometritis in gilts: Reproductive data, bacterial culture, histopathology, and infiltration <strong>of</strong> immune cells in the endometrium<br />
Source : Comparative Clinical Pathology, Volume 19, Issue 6, December <strong>2010</strong>, Pages 575-584<br />
Document Type : Article<br />
Keywords : Bacteria; Endometritis; Gilt; Immune cells; Reproduction<br />
<strong>Abstract</strong> : The aim <strong>of</strong> the present study was to quantify the number <strong>of</strong> immune cells infiltrated in the endometrium <strong>of</strong> endometritis<br />
gilts. Based on gross morphology, a selected 28 genital organs <strong>of</strong> endometritis gilts were investigated. The gilts were classified<br />
according to the ovarian appearance into three groups, i. e. follicular, luteal, and ovarian quiescent phases. Historical data,<br />
reason for culling, histopathology, bacterial identification, and number and type <strong>of</strong> immune cells in different layers <strong>of</strong> the<br />
endometrium were examined. The gilts were culled at 336 ± 63 days <strong>of</strong> age at a body weight <strong>of</strong> 142 ± 20 kg. The culling<br />
reasons included abnormal vaginal discharge (n = 10), repeat breeding (n = 6), anestrus (n = 6), abortion (n = 4), and not<br />
pregnant (n = 2). Bacteria identified from pus exudates included Escherichia coli (33.3%), Staphylococcus sp. (17.5%), αhemolytic<br />
Streptococcus sp. (14.3%), and β-hemolytic Streptococcus sp. (9.5%). Neutrophils were the most common<br />
immune cells in the epithelial and subepithelial tissue layers <strong>of</strong> the endometrium, while lymphocytes were the most<br />
common immune cells in the glandular layer. Neutrophils in the epithelial and subepithelial layers <strong>of</strong> the endometrium in<br />
the luteal phase were lower than in the follicular and ovarian quiescent phase. During the acute stage, neutrophils were the<br />
most common immune cells in the endometrium, while during the chronic stages, lymphocytes, plasma cells, and<br />
eosinophils were the dominant immune cells. In conclusion, the number and type <strong>of</strong> immune cells in the endometrium <strong>of</strong><br />
the endometritis gilts varied according to both the reproductive cycles and the stage <strong>of</strong> endometritis. Neutrophils,<br />
lymphocytes, plasma cells, and eosinophils indicate stages and the severity <strong>of</strong> endometritis. © 2009 Springer-Verlag London<br />
Limited.<br />
No.1563<br />
Author (s) : Tummaruk, P., Tienthai, P., Manee-In, S., Srisuwatanasagul, S.<br />
Title : Expression <strong>of</strong> progesterone receptor in the utero-tubal junction after intra-uterine and deep intra-uterine insemination in sows<br />
Source : Reproduction in Domestic Animals, Volume 45, Issue 5, October <strong>2010</strong>, Pages e26-e31<br />
Document Type : Article<br />
Keywords : progesterone, progesterone receptor; animal, animal disease, article, artificial insemination, female, gene expression<br />
regulation, genetics, metabolism, methodology, physiology, swine, uterine tube, uterus; Animals, Fallopian<br />
Tubes, Female, Gene Expression Regulation, Insemination, Artificial, Progesterone, Receptors, Progesterone, Swine, Uterus;<br />
Suidae<br />
<strong>Abstract</strong> : The aim <strong>of</strong> this study was to investigate the expression <strong>of</strong> progesterone receptor (PR) in the utero-tubal junction (UTJ) <strong>of</strong> sows<br />
at 24 h after intra-uterine insemination (IUI) and deep intra-uterine insemination (DIUI) compared with conventional artificial<br />
insemination (AI) in pigs. Fifteen multiparous sows were used: AI (n = 5), IUI (n = 5) and DIUI (n = 5). The sows were<br />
inseminated with a single dose <strong>of</strong> diluted semen during the second oestrus after weaning at 6-8 h prior to ovulation (AI: 3000<br />
× 106 spermatozoa, IUI: 1000 × 106 spermatozoa and DIUI: 150 × 106 spermatozoa). The UTJ was collected and subject to<br />
immunohistochemical staining using avidin-biotin immunoperoxidase technique with mouse monoclonal antibody to PR. In<br />
the oviductal part <strong>of</strong> the UTJ, the intensity <strong>of</strong> PR in the tunica muscularis and the proportion <strong>of</strong> PR-positive cells in the<br />
surface epithelium after DIUI were lower than AI (p < 0.05). The intensity and the proportion <strong>of</strong> PR-positive cells between AI<br />
and IUI in all compartments <strong>of</strong> the UTJ did not differ significantly (p > 0.05). When comparing between tissue<br />
compartments, prominent staining was observed in the muscular layer <strong>of</strong> the UTJ. It could be concluded that the expression<br />
<strong>of</strong> PR in the UTJ prior to fertilization after DIUI with a reduced number <strong>of</strong> spermatozoa was lower than that after AI. This<br />
might influence sperm transportation and the fertilization process. © 2009 Blackwell Verlag GmbH.<br />
No.1564<br />
Author (s) : Tungtrongchitr, A., Sookrung, N., Indrawattana, N., Kwangsi, S., Ongrotchanakun, J., Chaicumpa, W.<br />
Title : Giardia intestinalis in Thailand: Identification <strong>of</strong> genotypes<br />
Source : Journal <strong>of</strong> Health, Population and Nutrition, Volume 28, Issue 1, February <strong>2010</strong>, Pages 42-52<br />
Document Type : Article<br />
Keywords : ß-giardin; Genotyping; Giardia duodenalis; Giardia intestinalis; Giardiasis; Glutamate dehydrogenase; Thailand; Triose<br />
phosphate isomerase<br />
568
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : This study was undertaken to determine the genetic diversities <strong>of</strong> Giardia intestinalis isolated in Thailand. G. intestinalis cysts<br />
were collected from stool samples <strong>of</strong> 61 subjects residing in Bangkok or in rural communities <strong>of</strong> Thailand with and without<br />
gastrointestinal symptoms. All the cyst samples gave positive tpi amplicons (100% sensitivity), either <strong>of</strong> the 148-or the 81-bp<br />
tpi segments. Cyst assemblage identification <strong>of</strong> the 148- and 81-bp tpi gene segments by polymerase chain reaction showed<br />
that 8% <strong>of</strong> the cysts were assemblage A, 41% assemblage A and B combined, and 51% assemblage B. The prevalence <strong>of</strong><br />
assemblage A was significantly lower than that <strong>of</strong> assemblage B and the mixed types. Restriction fragment length<br />
polymorphism (RFLP) <strong>of</strong> the 384-bp ß-giardin gene segment revealed that 12% and 88% <strong>of</strong> the assemblage A cysts were AI<br />
and AII respectively. RFLP, based on the 432-bp gdh gene segment, showed 45.5% <strong>of</strong> the assemblage B cysts to be BIII and<br />
54.5% to be BIV. The AI sub-assemblage was less prevalent than the others. All subjects with AI and 50% <strong>of</strong> the subjects with<br />
BIII sub-assemblage cysts were symptomatic; 80% <strong>of</strong> symptomatic Bangkok residents were adults/elderly while 85% <strong>of</strong> the<br />
rural cases were children. © <strong>International</strong> Centre for Diarrhoeal Disease Research, Bangladesh.<br />
No.1565<br />
Author (s) : Tungtrongchitr, R., Phonrat, B., Yamborisut, U., Popruk, S., Tungtrongchitr, A.<br />
Title : Relationship between α-MSH, soluble leptin receptor, MC4R polymorphism and BMI in Thai children and adolescents<br />
Source : Journal <strong>of</strong> Pediatric Biochemistry, Volume 1, Issue 4, <strong>2010</strong>, Pages 303-308<br />
Document Type : Article<br />
Keywords : α-melanocortin stimulating hormone; body mass index; melanocortin -4 receptor; soluble leptin receptor<br />
<strong>Abstract</strong> : In order to evaluate the mechanism <strong>of</strong> disturbance <strong>of</strong> melanocortin signaling in different groups <strong>of</strong> Thai children and<br />
adolescents classified according to body mass index (BMI) percentile for age by The Centers for Disease Control and<br />
Prevention (CDC) growth charts, and α-melanocortin stimulating hormone (α-MSH), soluble leptin receptor and melanocortin<br />
-4 receptor (MC4R) polymorphism were studied. 149 male and 127 female children and adolescent Thai subjects with age<br />
range between 5-19 years were selected. Statistically significant difference <strong>of</strong> serum leptin receptor concentration between<br />
under weight (< 5th percentile), possible at risk for underweight (between 5th and 15th percentile), probably healthy<br />
(between 15th and 85th percentile); at risk for overweight (between the 85th and 95th percentile) and overweight (over the<br />
95th percentile) were found. The levels <strong>of</strong> α-MSH were not significantly different when comparing the groups. Heterozygous<br />
<strong>of</strong> MC4R polymorphism (Val103Ile) were found in underweight, possible at risk for underweight and probably healthy group<br />
which might have been the cause <strong>of</strong> lower BMI for age even they have had normal serum α-MSH concentration. Using the<br />
logistic regression analysis, the group <strong>of</strong> risk for being overweight and overweight was statistically significant with α-MSH. This<br />
study supported a positive correlation between α-MSH and BMI with ages <strong>of</strong> Thai children and adolescents group. ©<br />
<strong>2010</strong>/2011 - IOS Press and the authors. All rights reserved.<br />
No.1566<br />
Author (s) : Tunpiboonsak, S., Mongkolrob, R., Kitudomsub, K., Thanwatanaying, P., Kiettipirodom, W., Tungboontina, Y., Tungpradabkul, S.<br />
Title : Role <strong>of</strong> a Burkholderia pseudomallei polyphosphate kinase in an oxidative stress response, motilities, and bi<strong>of</strong>ilm formation<br />
Source : Journal <strong>of</strong> Microbiology, Volume 48, Issue 1, March <strong>2010</strong>, Pages 63-70<br />
Document Type : Article<br />
Keywords : B. mallei; B. pseudomallei; Bi<strong>of</strong>ilm; Flagellin; Oxidative stress; Polyphosphate kinase<br />
<strong>Abstract</strong> : Burkholderia pseudomallei, a motile and rod Gram-negative bacterium, is the causative agent <strong>of</strong> melioidosis. The bacterium is<br />
an intracellular pathogen and that motility is generally crucial for their survival in a natural environment and for systemic<br />
infection inside a host. We report here a role <strong>of</strong> B. pseudomallei polyphosphate kinase in virulence, such as an oxidative<br />
stress response, motilities and bi<strong>of</strong>ilm formation. The polyphosphate kinase (ppk) mutant is susceptible to hydrogen peroxide<br />
in an oxidative stress condition, unable to perform swimming, swarming motilities, and has lower density bi<strong>of</strong>ilm forming<br />
capacity than the wild-type strain. We also demonstrated that both polyphosphate kinase and motile flagella are essential<br />
and independently involved in bi<strong>of</strong>ilm formation. The B. pseudomallei flagellin (fliC) mutant and B. mallei, a nonmotile<br />
species, are shown to produce higher density bi<strong>of</strong>ilm formation than the ppk mutant, but less than wild type B.<br />
pseudomallei. © <strong>2010</strong> The Microbiological Society <strong>of</strong> Korea.<br />
No.1567<br />
Author (s) : Tunthong, R.; Vathesatogkit, P.; Yamwong, S.; Sukasem, C.; Chantratita, W.; Angchaisuksiri, P.; Chuncharunee, S.; Sura, T.;<br />
Boonbaichaiyapruck, S.; Sritara, P.<br />
569
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Inverse effect <strong>of</strong> cytochrome P450 2C19 polymorphisms on platelet response in clopidogrel low-responsive patient receiving<br />
higher dose adjustment<br />
Source : EUROPEAN HEART JOURNAL, Volume: 31, Supplement: 1, Pages: 969-969, Published: SEP <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1568<br />
Author (s) : Tuntikulwattana, S., Mitrevej, A., Kerdcharoen, T., Williams, D.B., Sinchaipanid, N.<br />
Title : Development and optimization <strong>of</strong> micro/nanoporous osmotic pump tablets<br />
Source : AAPS PharmSciTech, Volume 11, Issue 2, June <strong>2010</strong>, Pages 924-935<br />
Document Type : Article<br />
Keywords : Drug release; Micro/nanoporous semipermeable membrane; Osmotic pump tablet; PVP; Response surface methodology<br />
<strong>Abstract</strong> : Micro/nanoporous osmotic pump tablets coated with cellulose acetate containing polyvinylpyrolidone (PVP) as pore formers<br />
were fabricated. Propranolol hydrochloride was used as a model drug in this study. Formulation optimization based on USP<br />
31 requirements was conducted following a central composite design using a two-level factorial plan involving two<br />
membrane variables (pore former and coating levels). Effect <strong>of</strong> molecular weight <strong>of</strong> pore former (PVP K30 and PVP K90) was<br />
also evaluated. Responses <strong>of</strong> drug release to the variables were analyzed using statistical s<strong>of</strong>tware (MINITAB 14). Scanning<br />
electron microscopy and atomic force microscopy showed that the pores formed by PVP. The drug release was dependent<br />
on the molecular weight and concentration <strong>of</strong> PVP and the level <strong>of</strong> coating. The results showed that acceptable 12-h pr<strong>of</strong>ile<br />
could be achieved with only specific range <strong>of</strong> PVP K30-containing membrane at the defined membrane thickness. However,<br />
satisfactory 24-h pr<strong>of</strong>ile could be accomplished by both PVP K30 and PVP K90-containing membrane at the range and<br />
membrane thickness tested. Preparation and testing <strong>of</strong> the optimized formulation showed a good correlation between<br />
predicted and observed values. © <strong>2010</strong> American Association <strong>of</strong> Pharmaceutical Scientists.<br />
No.1569<br />
Author (s) : Turathum, B., Saikhun, K., Sangsuwan, P., Kitiyanant, Y.<br />
Title : Effects <strong>of</strong> vitrification on nuclear maturation, ultrastructural changes and gene expression <strong>of</strong> canine oocytes<br />
Source : Reproductive Biology and Endocrinology, Volume 8, 22 June <strong>2010</strong>, Article number 70<br />
Document Type : Article<br />
Keywords : copper zinc superoxide dismutase, DNA methyltransferase 1, fat droplet, heat shock protein 70, protein Bax, protein bcl<br />
2, cryoprotective agent; animal cell, article, cell granule, cell ultrastructure, controlled<br />
study, cryopreservation, dog, female, gene expression, germinal vesicle, in vitro study, meiosis, metaphase, nonhuman, oocyte<br />
maturation, reverse transcription polymerase chain reaction, smooth endoplasmic reticulum, structure analysis, transmission<br />
electron microscopy, vitrification, animal, cell culture, cell nucleus, cell survival, comparative study, drug<br />
effect, evaluation, genetics, metabolism, oocyte, oocyte development, physiology, ultrastructure; Canidae; Animals, Cell<br />
Nucleus, Cell Survival, Cells, Cultured, Cryopreservation, Cryoprotective Agents, Dogs, Female, Gene<br />
Expression, Meiosis, Oocytes, Oogenesis<br />
<strong>Abstract</strong> : Background: Cryopreservation <strong>of</strong> oocytes, which is an interesting procedure to conserve female gametes, is an essential part<br />
<strong>of</strong> reproductive biotechnology. The objective <strong>of</strong> the present study was to investigate the effects <strong>of</strong> vitrification on nuclear<br />
maturation, ultrastructural changes and gene expression <strong>of</strong> canine oocytes.Methods: Immature oocytes (germinal vesicles)<br />
isolated from ovaries <strong>of</strong> normal bitches (> 6 months <strong>of</strong> age) were either vitrified in open pulled straw (OPS) using 20%<br />
ethylene glycol (EG) and 20% dimethyl sulfoxide (DMSO) as vitrification solution or exposed to vitrification solution without<br />
subjected to liquid nitrogen. After warming, oocytes were investigated for nuclear maturation following in vitro maturation<br />
(IVM), ultrastructural changes using transmission electron microscopy (TEM) and gene expression using RT-PCR. Fresh immature<br />
oocytes were used as the control group.Results: The rate <strong>of</strong> resumption <strong>of</strong> meiosis in vitrified-warmed oocytes (53.4%) was<br />
significantly (P < 0.05) lower than those <strong>of</strong> control (93.8%) and exposure (91.4%) groups. However, there were no statistically<br />
significant differences among groups in the rates <strong>of</strong> GV oocytes reaching the maturation stage (metaphase II, MII). The<br />
ultrastructural alterations revealed by TEM showed that cortical granules, mitochondria, lipid droplets and smooth<br />
endoplasmic reticulum (SER) were affected by vitrification procedures. RT-PCR analysis for gene expression revealed no<br />
differences in HSP70, Dnmt1, SOD1 and BAX genes among groups, whereas Bcl2 was strongly expressed in vitrified-warmed<br />
group when compared to the control.Conclusion: Immature canine oocytes were successfully cryopreserved, resumed<br />
570
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
meiosis and developed to the MII stage. The information obtained in this study is crucial for the development <strong>of</strong> an effective<br />
method to cryopreserve canine oocytes for establishment <strong>of</strong> genetic banks <strong>of</strong> endangered canid species. © <strong>2010</strong> Turathum et<br />
al; licensee BioMed Central Ltd.<br />
No.1570<br />
Author (s) : Turbpaiboon, C., Wilairat, P.<br />
Title : Alpha-hemoglobin stabilizing protein: Molecular function and clinical correlation<br />
Source : Frontiers in Bioscience, Volume 15, Issue 1, 1 January <strong>2010</strong>, Pages 1-11<br />
Document Type : Article<br />
Keywords : AHSP; Alpha-Hb; Alpha-hemoglobin; Alpha-hemoglobin stabilizing protein; Hb; Hemoglobin; Hemoglobin<br />
variant; Molecular chaperone; Review; Thalassemia<br />
<strong>Abstract</strong> : The discovery <strong>of</strong> alpha-hemoglobin stabilizing protein (AHSP), a chaperone for free alpha-hemoglobin (alpha-Hb), has<br />
provided a satisfactory solution to the perplexing problem <strong>of</strong> balanced globin levels for Hb production in erythroid cells in<br />
the face <strong>of</strong> a two-fold excess <strong>of</strong> alpha-globin to beta-globin gene dosage. Unmatched alpha-Hb is unstable and precipitates<br />
onto membranes, where the released heme exerts oxidative damages resulting in ineffective erythropoiesis and hemolytic<br />
anemia, the underlying causes <strong>of</strong> pathology in the hereditary anemia <strong>of</strong> beta-thalassemia. The interaction <strong>of</strong> alpha-Hb with<br />
AHSP involves surfaces normally employed in binding to beta-Hb. However, a conformational change to the AHSP-bound<br />
alpha-Hb results in an oxidized heme, but in a pocket that is now less exposed to the outside environment, thereby<br />
protecting against both peroxide-induced heme loss and iron-induced redox reaction. Studies in both mice and humans<br />
indicate that reduction in AHSP can result in hematological pathology. Conversely, alpha-Hb variants that are compromised in<br />
their ability to bind with AHSP produce beta-thalassemia-like symptoms. Disease conditions like some forms <strong>of</strong> thalassemia<br />
that are directly associated with AHSP structural and/or functional defects can now be included within the category <strong>of</strong><br />
chaperonopathies.<br />
No.1571<br />
Author (s) : Ubol, S., Halstead, S.B.<br />
Title : How innate immune mechanisms contribute to antibody-enhanced viral infections<br />
Source : Clinical and Vaccine Immunology, Volume 17, Issue 12, December <strong>2010</strong>, Pages 1829-1835<br />
Document Type : Short Survey<br />
Keywords : antibody, B7 antigen, CD40 antigen, CD86 antigen, interferon, measles vaccine, respiratory syncytial virus vaccine, toll like<br />
receptor; cell maturation, dendritic cell, dengue, disease severity, human, immune complex deposition, immunization, innate<br />
immunity, macrophage, monocyte, priority journal, short survey, Th2 cell, virus entry, virus infection, virus replication;<br />
Animals, Antibodies, Blocking, Antibody-Dependent Enhancement, Humans, Immune Tolerance, Immunity, Innate, Viral<br />
Vaccines, Virus Diseases, Viruses<br />
<strong>Abstract</strong> : Preexisting antibodies may enhance viral infections. In dengue, nonneutralizing antibodies raised by natural infection with one<br />
<strong>of</strong> four dengue viruses (DENVs) may enhance infection with a different virus by a process we term "intrinsic antibodydependent<br />
enhancement" (iADE). In addition, nonprotective antibodies raised by formalin-inactivated respiratory syncytial<br />
virus (RSV) and measles virus vaccines have led to enhanced disease during breakthrough infections. Infections under iADE<br />
conditions not only facilitate the process <strong>of</strong> viral entry into monocytes and macrophages but also modify innate and adaptive<br />
intracellular antiviral mechanisms, suppressing type 1 interferon (IFN) production and resulting in enhanced DENV replication.<br />
The suppression observed in vitro has been documented in patients with severe (dengue hemorrhagic fever [DHF]) but not in<br />
patient with mild (dengue fever [DF]) secondary dengue virus infections. Important veterinary viral infections also may exhibit<br />
iADE. It is thought that use <strong>of</strong> formalin deconforms viral epitopes <strong>of</strong> RSV, resulting in poor Toll-like receptor (TLR) stimulation;<br />
suboptimal maturation <strong>of</strong> dendritic cells with reduced production <strong>of</strong> activation factors CD40, CD80, and CD86; decreased<br />
germinal center formation in lymph nodes; and the production <strong>of</strong> nonprotective antibodies. These antibodies fail to<br />
neutralize RSV, allowing replication with secondary stimulation <strong>of</strong> RSV-primed Th2 cells producing more low-avidity antibody,<br />
resulting in immune complexes deposited into affected tissue. However, when formalin-inactivated RSV was administered<br />
with a TLR agonist to mice, they were protected against wild-type virus challenge. Safe and effective vaccines against<br />
RSV/measles virus and dengue virus may benefit from a better understanding <strong>of</strong> how innate immune responses can promote<br />
production <strong>of</strong> protective antibodies. Copyright © <strong>2010</strong>, American Society for Microbiology. All Rights Reserved.<br />
571
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.1572<br />
Author (s) : Ubol, S., Phuklia, W., Kalayanarooj, S., Modhiran, N.<br />
Title : Mechanisms <strong>of</strong> immune evasion induced by a complex <strong>of</strong> dengue virus and preexisting enhancing antibodies<br />
Source : Journal <strong>of</strong> Infectious Diseases, Volume 201, Issue 6, <strong>2010</strong>, Pages 923-935<br />
Document Type : Article<br />
Keywords : beta interferon, complementary DNA, cytokine, Fc receptor, inducible nitric oxide synthase, interferon, interleukin 10, Janus<br />
kinase, protein mda 5, retinoic acid inducible protein I, small interfering RNA, STAT protein, unclassified drug;<br />
article, autophagy, cell activation, cell differentiation, cytokine production, Dengue virus, DNA microarray, down<br />
regulation, gene expression, genetic transcription, human, immune response, interferon production, nonhuman, priority<br />
journal, protein expression, signal transduction, upregulation, virus entry, virus replication; Antibodies, Blocking, Antibodies,<br />
Viral, Cell Line, Cytokines, DEAD-box RNA Helicases, Dengue, Dengue Virus, Enzyme-Linked Immunosorbent<br />
Assay, Humans, Interferon-beta, Interleukin-10, Janus Kinases, Reverse Transcriptase Polymerase Chain Reaction, RNA, Small<br />
Interfering, RNA, Viral, Signal Transduction, STAT1 Transcription Factor, Thailand, Virus Replication<br />
<strong>Abstract</strong> : We have found that dengue virus (DENV) not only uses preexisting enhancing antibodies to promote its entry into Fc<br />
receptor-bearing cells but also exploits enhancing antibodies for intracellular immune evasion through 2 mechanisms. In the<br />
first mechanism, entry <strong>of</strong> DENV-antibody complexes into human monocytic cells activates negative regulators,<br />
dihydroxyacetone kinase and autophagy-related 5-autophagy-related 12, which then disrupt the retinoic acide incucible gene I<br />
and melanoma differentiation associated gene 5 signaling cascade and disable type 1 interferon production, leading to<br />
suppression <strong>of</strong> interferon-mediated antiviral responses. In the second mechanism, the immune evasion was found to be<br />
mediated by the suppressive cytokine interleukin 10 (IL-10). High levels <strong>of</strong> IL-10 activated expression <strong>of</strong> suppressor <strong>of</strong> cytokine<br />
signaling 3 gene, which subsequently inactivated the Janus kinase-signal transducer and activator <strong>of</strong> transcription pathway.<br />
Inhibition <strong>of</strong> IL-10 production by small interfering RNA down-regulated suppressor <strong>of</strong> cytokine signaling 3 gene expression,<br />
restored inducible nitric oxide synthase gene expression, and suppressed DENV replication. Importantly, we were able to<br />
demonstrate that these 2 loops <strong>of</strong> suppression occurred in patients with severe secondary dengue infection (dengue<br />
hemorrhagic fever) but not in patients with mild secondary dengue infection (dengue fever). © <strong>2010</strong> by the Infectious<br />
Diseases Society <strong>of</strong> America. All rights reserved.<br />
No.1573<br />
Author (s) : Ubolchonlakate, K., Sikong, L., Tontai, T.<br />
Title : Formaldehyde degradation by photocatalytic Ag-Doped TiO2 film <strong>of</strong> glass fiber roving<br />
Source : Journal <strong>of</strong> Nanoscience and Nanotechnology, Volume 10, Issue 11, November <strong>2010</strong>, Pages 7522-7525<br />
Document Type : Conference Paper<br />
Keywords : Ag doped TiO2; Formaldehyde degradation; Photocatalytic; Porous film; Thin film<br />
<strong>Abstract</strong> : The photocatalytic Ag doped TiO2 porous films were prepared by sol-gel method and dip coated on glass fiber roving. The<br />
sol composed <strong>of</strong> titanium (IV) isopropoxide, triethanolamine, ethanol and nitric acid followed by calcination <strong>of</strong> the film at 500<br />
°C for 1 hour with a heating rate <strong>of</strong> 3 °C/min. The surface morphology and properties <strong>of</strong> synthesized TiO2 films were<br />
characterized by X-ray diffraction, atomic forced microscope and scanning electron microscope. A laboratory photocatalytic<br />
reactor was set up to carry out photoactivity <strong>of</strong> the prepared catalysts. The results show that TiO2-Ag and TiO2-Ag-TEA porous<br />
films give highest rate <strong>of</strong> formaldehyde gas degradation. It can be noted that triethanolamine exhibits two effects on TiO2<br />
composite films; one is its effect on porous film structure and second is a reverse effect <strong>of</strong> hindrance <strong>of</strong> anatase growth.<br />
Copyright © <strong>2010</strong> American Scientific Publishers.<br />
No.1574<br />
Author (s) : Uchaipichat, V., Suthisisang, C., Mackenzie, P.I., Miners, J.O.<br />
Title : Kinetic and Enzymatic Characterisation <strong>of</strong> Human Hepatic R- and S- Lorazepam Glucuronidation<br />
Source : DRUG METABOLISM REVIEWS, Volume: 42, Supplement: 1, Pages: 64-65, Published: AUG <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1575<br />
Author (s) : Udonsom, R., Buddhirongawatr, R., Sukthana, Y.<br />
572
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Is sabin-feldman dye test using T. Gondii tachyzoites from animal inoculation still the best method for detecting toxoplasma<br />
gondii antibodies?<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 5, September <strong>2010</strong>, Pages 1059-1064<br />
Document Type : Article<br />
Keywords : Dye test; IFAT; Mouse and cell culture derived tachyzoites; Toxoplasma gondii<br />
<strong>Abstract</strong> : Although the Sabin-Feldman dye test is the gold standard for detecting Toxoplasma antibodies in human, it is performed only<br />
in reference laboratories because live virulent T. gondii are used for the test. We collected 210 human serum samples and<br />
tested them by the dye test using in vivo tachyzoites (conventional method) then compared these results with three other<br />
methods: a dye test using cell culture-derived T. gondii tachyzoites and indirect immun<strong>of</strong>luorescent antibody tests (IFAT)<br />
using in vivo and in vitro tachyzoites. We found the conventional dye test detected the highest percent <strong>of</strong> cases (4.3%),<br />
followed by the IFAT using parasites from mice (3.8%), then the dye test and the IFAT using cell culture tachyzoites (both<br />
2.8%). Agreement with the dye test when using mouse and cell culture derived tachyzoites was 96.7%. Using in vivo<br />
tachyzoites for the dye test and the IFAT gave 94.3% agreement, while using in vitro tachyzoites gave 94.8% agreement.<br />
When compared with the conventional dye test, the IFAT had 75% sensitivity and 100% specificity. The T. gondii tachyzoites<br />
obtained from cell culture had a lower virulence, as indicated by a three times longer survival period in the inoculated mice.<br />
We favor the conventional dye test as the gold standard for Toxoplasma antibody detection. In vitro tachyzoites can be used<br />
routinely in the dye test but false negative results may occur in some cases. The IFAT, using either in vivo or in vitro<br />
tachyzoites, are alternatives for laboratories where provision <strong>of</strong> live tachyzoites is limited.<br />
No.1576<br />
Author (s) : Unchern, S., Laohareungpanya, N., Sanvarinda, Y., Pattanapanyasat, K., Tanratana, P., Chantharaksri, U., Sibmooh, N.<br />
Title : Oxidative modification and poor protective activity <strong>of</strong> HDL on LDL oxidation in thalassemia<br />
Source : Lipids, Volume 45, Issue 7, July <strong>2010</strong>, Pages 627-633<br />
Document Type : Article<br />
Keywords : HDL; LDL oxidation; Oxidative stress; Oxidized HDL; Thalassemia<br />
<strong>Abstract</strong> : Oxidative modification <strong>of</strong> low-density lipoprotein (LDL) has been reported in thalassemia, which is a consequence <strong>of</strong> oxidative<br />
stress. However, the levels <strong>of</strong> oxidized high-density lipoprotein (HDL) in thalassemia have not been evaluated and it is unclear<br />
whether HDL oxidation may be linked to LDL oxidation. In this study, the levels <strong>of</strong> total cholesterol, iron, protein, conjugated<br />
diene (CD), lipid hydroperoxide (LOOH), and thiobarbituric acid reactive substances (TBARs) were determined in HDL from<br />
healthy volunteers and patients with β-thalassemia intermedia with hemoglobin E (β-thal/Hb E). The protective activity <strong>of</strong><br />
thalassemic HDL on LDL oxidation was also investigated. The iron content <strong>of</strong> HDL2 and HDL3 from β-thal/HbE patients was<br />
higher while the cholesterol content was lower than those in healthy volunteers. Thalassemic HDL2 and HDL3 had increased<br />
levels <strong>of</strong> lipid peroxidation markers i.e., conjugated diene, LOOH, and TBARs. Thalassemic HDL had lower peroxidase activity<br />
than control HDL and was unable to protect LDL from oxidation induced by CuSO4. Our findings highlight the oxidative<br />
modification and poor protective activity <strong>of</strong> thalassemic HDL on LDL oxidation which may contribute to cardiovascular<br />
complications in thalassemia. © <strong>2010</strong> AOCS.<br />
No.1577<br />
Author (s) : Ungkanont, K., Charuluxananan, S., Komoltri, C.<br />
Title : Association <strong>of</strong> otoscopic findings and hearing level in pediatric patients with otitis media with effusion<br />
Source : <strong>International</strong> Journal <strong>of</strong> Pediatric Otorhinolaryngology, Volume 74, Issue 9, September <strong>2010</strong>, Pages 1063-1066<br />
Document Type : Article<br />
Keywords : Hearing loss; Otitis media with effusion; Otoscopy; Tympanic membrane<br />
<strong>Abstract</strong> : Objective: To find the association between the abnormalities <strong>of</strong> tympanic membrane characteristics and the hearing level in<br />
pediatric patients with otitis media with effusion. Methods: Sixty-three pediatric patients with otitis media with effusion had<br />
undergone ear examinations by pneumatic otoscopy to assess the color, transparency, mobility, fluid level and retraction <strong>of</strong><br />
the tympanic membrane. An audiogram was done in the same setting, average hearing threshold and air-bone gap were<br />
measured. Otoscopic findings and the result <strong>of</strong> the hearing test were analyzed to identify the association between the<br />
abnormalities <strong>of</strong> the tympanic membrane characteristics and elevated hearing threshold. Results: Hearing loss was found in<br />
92.1% <strong>of</strong> the patients. Mean hearing level was 31.7 ± 10.3. dB. From linear regression analysis, the patients with dull or<br />
opaque tympanic membrane had a significantly higher hearing threshold <strong>of</strong> 7.2. dB than the patient with translucent ear drum<br />
after adjusting for mobility and retraction. The patients with tympanic membrane retraction had a higher hearing threshold <strong>of</strong><br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
5.1. dB than the patient who had no retraction after adjusting for transparency and mobility. Mobility had a significant<br />
relationship to elevated hearing threshold in the univariate analysis but not in multivariable analysis. Conclusion: Opacity and<br />
retraction were the two characteristics <strong>of</strong> abnormal tympanic membrane that were associated with elevated hearing<br />
threshold in the patients with otitis media with effusion. Hearing test is suggested if opacity or retraction <strong>of</strong> the tympanic<br />
membrane is found. © <strong>2010</strong> Elsevier Ireland Ltd.<br />
No.1578<br />
Author (s) : Ungsupravate, D., Sawasdee, N., Khositseth, S., Udomchaiprasertkul, W., Khoprasert, S., Li, J., Reithmeier, R.A.F.,<br />
Yenchitsomanus, P.-T.<br />
Title : Impaired trafficking and intracellular retention <strong>of</strong> mutant kidney anion exchanger 1 proteins (G701D and A858D) associated<br />
with distal renal tubular acidosis<br />
Source : Molecular Membrane Biology, Volume 27, Issue 2-3, 17 April <strong>2010</strong>, Pages 92-103<br />
Document Type : Article<br />
Keywords : Anion exchanger 1 (AE1); Band 3; Distal renal tubular acidosis (dRTA); Membrane protein trafficking; SLC4A1<br />
<strong>Abstract</strong> : Novel compound heterozygous mutations, G701D, a recessive mutation, and A858D, a mild dominant mutation, <strong>of</strong> human<br />
solute carrier family 4, anion exchanger, member 1 (SLC4A1) were identified in two pediatric patients with distal renal tubular<br />
acidosis (dRTA). To examine the interaction, trafficking, and cellular localization <strong>of</strong> the wild-type and two mutant kidney AE1<br />
(kAE1) proteins, we expressed the proteins alone or together in human embryonic kidney (HEK) 293T and Madin-Darby canine<br />
kidney (MDCK) epithelial cells. In individual expressions, wild-type kAE1 was localized at the cell surface <strong>of</strong> HEK 293T and the<br />
basolateral membrane <strong>of</strong> MDCK cells. In contrast, kAE1 G701D was mainly retained intracellularly, while kAE1 A858D was<br />
observed intracellularly and at the cell surface. In co-expression experiments, wild-type kAE1 formed heterodimers with kAE1<br />
G701D and kAE1 A858D, and promoted the cell surface expression <strong>of</strong> the mutant proteins. The co-expressed kAE1 G701D and<br />
A858D could also form heterodimers but showed predominant intracellular retention in HEK 293T and MDCK cells. Thus<br />
impaired trafficking <strong>of</strong> the kAE1 G701D and A858D mutants would lead to a pr<strong>of</strong>ound decrease in functional kAE1 at the<br />
basolateral membrane <strong>of</strong> α-intercalated cells in the distal nephron <strong>of</strong> the patients with dRTA. © <strong>2010</strong> Informa UK Ltd.<br />
No.1579<br />
Author (s) : Unhasuta, K., Robinson, M.V., Magilvy, K.<br />
Title : Research plan for developing trauma core competencies for nurses in Thailand<br />
Source : <strong>International</strong> Emergency Nursing, Volume 18, Issue 1, January <strong>2010</strong>, Pages 3-7<br />
Document Type : Article<br />
Keywords : Core competency; Emergency department; Thailand; Trauma nurse<br />
<strong>Abstract</strong> : The aim <strong>of</strong> this research plan was identification and development <strong>of</strong> core competencies for emergency trauma nurses in<br />
Thailand. The research plan was undertaken in three phases. Phase I: a national survey <strong>of</strong> the critical dimensions <strong>of</strong> care;<br />
Phase II: development <strong>of</strong> a 64 item tool; and Phase III: efficiency study with emergency nurses representing Level-1, Regional,<br />
Community and Rural facilities. Six dimensions <strong>of</strong> competency were identified: cooperation, decision-making, leadership,<br />
problem-solving, teamwork, and technical knowledge with a content validity index (CVI) <strong>of</strong> 1.00 and internal consistency<br />
(Cronbach's α) <strong>of</strong> 0.98 (N = 485 RNs in 29 hospitals). Analysis <strong>of</strong> the first year pilot study data using a 5 point likert scale (N =<br />
285 RNs in 16 hospitals), nurses rated themselves as 4.18 (SD = 0.69) and their peers at 4.00 (SD = 0.68). Head nurse<br />
evaluations averaged 3.98 (SD = 0.55). ANOVA demonstrated no statistical significance (p = 0.09) between groups. The results<br />
<strong>of</strong> the preliminary studies demonstrated appropriateness <strong>of</strong> the core competency items, but refinement is required prior to<br />
national distribution. © 2009 Elsevier Ltd. All rights reserved.<br />
No.1580<br />
Author (s) : Unnanuntana, A., Murphy, J.E., Petersilge, W.J.<br />
Title : Management <strong>of</strong> chronic lateral instability due to lateral collateral ligament deficiency after total knee arthroplasty: A case<br />
report<br />
Source : Journal <strong>of</strong> Medical Case Reports, Volume 4, <strong>2010</strong>, Article number 144<br />
Document Type : Article<br />
Keywords : polyethylene; adult, antibiotic therapy, article, case report, chronic disease, clinical feature, disease course, equipment<br />
design, female, follow up, human, immobilization, knee dislocation, knee instability, knee ligament injury, knee<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
prosthesis, knee radiography, ligament surgery, long term care, obesity, postoperative infection, priority<br />
journal, reoperation, salvage therapy, Staphylococcus aureus, surgical technique, total knee replacement, treatment failure<br />
<strong>Abstract</strong> : Introduction. Lateral instability following total knee arthroplasty (TKA) is a rare condition with limited report <strong>of</strong> treatment<br />
options. The objective <strong>of</strong> this case presentation is to demonstrate the outcomes <strong>of</strong> different surgical procedures performed in<br />
a single patient with lateral collateral ligament (LCL) deficiency. Case presentation. We present a case <strong>of</strong> chronic lateral<br />
instability due to LCL deficiency after primary TKA in a 47-year-old Caucasian woman with an obesity problem. Multiple<br />
treatment options have been performed in order to manage this problem, including the following: ligament reconstruction;<br />
combined ligament reconstruction and constrained implant; and rotating-hinge knee prosthesis that was the most recent<br />
surgery. All ligament reconstruction procedures failed within one year. The varus-valgus constrained prosthesis provided<br />
stability for six years. Conclusions. Ligament reconstruction alone cannot provide enough stability for the treatment <strong>of</strong> chronic<br />
lateral instability in patients with obesity problems and LCL deficiency. When the reconstruction fails, a salvage procedure<br />
with rotating-hinge knee is still available. © <strong>2010</strong> Unnanuntana et al; licensee BioMed Central Ltd.<br />
No.1581<br />
Author (s) : Untang, M., Shiowatana, J., Siripinyanond, A.<br />
Title : A simple cyanide test kit for water and fruit juices<br />
Source : Analytical Methods, Volume 2, Issue 11, November <strong>2010</strong>, Pages 1698-1701<br />
Document Type : Article<br />
Keywords : Dianions, Field test, Gaseous hydrogen, Hydrogen Cyanide, Natural waters, P-nitrobenzaldehyde, Sodium hydroxides, Standard<br />
method, Test solutions; Aldehydes, Fruit juices, Fruits, Hydrogen, Reaction kinetics, Sodium, Sulfuric acid, Testing; Cyanides<br />
<strong>Abstract</strong> : A sensitive, selective, rapid, and user-friendly field test kit was developed for the determination <strong>of</strong> cyanide. The method was<br />
based on a reaction to generate hydrogen cyanide from the solution containing cyanide by reaction with sulfuric acid in a<br />
specially designed reaction bottle. The gaseous hydrogen cyanide diffused and was collected on a paper strip impregnated<br />
with p-nitrobenzaldehyde and o-dinitrobenzene in sodium hydroxide. Hydrogen cyanide reacted with p-nitrobenzaldehyde to<br />
form an intermediate p-cyanobenzadehyde cyanohydrin, which reacted with o-dinitrobenzene to give a purple compound,<br />
the dianion <strong>of</strong> o-nitrophenyl hydroxylamine. The intensity <strong>of</strong> the purple color depended on the concentration <strong>of</strong> cyanide in<br />
the test solution. The developed field test kit could be used for the detection <strong>of</strong> cyanide as low as 0.01 mg l-1 within 10 min.<br />
The cyanide field test kit was applied to determine cyanide concentrations in natural water and commercial fruit juices with<br />
good accuracy as validated by using the APHA/AWWA/WEF standard method 4500-CN-. © <strong>2010</strong> The Royal Society <strong>of</strong><br />
Chemistry.<br />
No.1582<br />
Author (s) : Upadhyay, B.P., Utrarachkij, F., Thongshoob, J., Mahakunkijcharoen, Y., Wongchinda, N., Suthienkul, O., Khusmith, S.<br />
Title : Detection <strong>of</strong> salmonella invA gene in shrimp enrichment culture by polymerase chain reaction<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 2, March <strong>2010</strong>, Pages 426-435<br />
Document Type : Article<br />
Keywords : Enrichment culture; Polymerase chain reaction; Salmonella; Shrimp<br />
<strong>Abstract</strong> : Contamination <strong>of</strong> seafood with salmonellae is a major public health concern. Detection <strong>of</strong> Salmonella by standard culture<br />
methods is time consuming. In this study, an enrichment culture step prior to polymerase chain reaction (PCR) was applied to<br />
detect 284 bp fragment <strong>of</strong> Salmonella invA in comparison with the conventional culture method in 100 shrimp samples<br />
collected from four different shrimp farms and fresh food markets around Bangkok. Samples were pre-enriched in nonselective<br />
lactose broth (LB) and selective tetrathionate broth (TTB). PCR detection limit was 10 pg and 104 cfu/ml <strong>of</strong> viable<br />
salmonellae with 100% specificity. PCR assay detected 19 different Salmonella serovars belonging to 8 serogroups (B, C1, C2-<br />
C3, D1, E1, E4 and K) commonly found in clinical and environmental samples in Thailand. The detection rate <strong>of</strong> PCR following<br />
TTB enrichment (24%) was higher than conventional culture method (19%). PCR following TTB, but not in LB enrichment<br />
allowed salmonella detection with 84% sensitivity, 90% specificity and 89% accuracy. Shrimp samples collected from fresh<br />
food markets had higher levels <strong>of</strong> contaminated salmonellae than those from shrimp farms. The results indicated that<br />
incorporation <strong>of</strong> an enrichment step prior to PCR has the potential to be applied for detection <strong>of</strong> naturally contaminated<br />
salmonellae in food, environment and clinical samples.<br />
No.1583<br />
Author (s) : U-Pratya, Y., Lueangamornnara, U., Jiratchariyakul, W., Kummalue, T.<br />
575
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Immunosuppressive effects <strong>of</strong> cucurbitacin B on human peripheral blood lymphocytes<br />
Source : Journal <strong>of</strong> Medicinal Plant Research, Volume 4, Issue 22, 18 November <strong>2010</strong>, Pages 2340-2347<br />
Document Type : Article<br />
Keywords : CD69; Cucurbitacin b; Cytotoxic lymphocyte function; Phytohemagglutinin stimulated peripheral blood mononuclear cell<br />
<strong>Abstract</strong> : This study investigates the immunomodulating effects <strong>of</strong> cucurbitacin B, a Thai herbal plant; isolated from Trichosanthes<br />
cucumerima L., on PHA-stimulated human Peripheral Blood Mononuclear Cells (PBMCs). Stimulated PBMCs were cultured in<br />
the presence or absence <strong>of</strong> cucurbitacin B and then markers <strong>of</strong> cell activation were measured. The results showed that 0.5<br />
and 0.25 μg/ml <strong>of</strong> cucurbitacin B significantly inhibited the expression <strong>of</strong> CD69+CD3+, and CD25+CD3+ whereas no significant<br />
inhibition from 0.05 μg/ml <strong>of</strong> cucurbitacin B was detected. The secretion <strong>of</strong> TNF-α from stimulated PBMC was significantly<br />
inhibited by cucurbitacin B. After 24 h <strong>of</strong> incubation, the total Cytotoxic Lymphocyte (CTL) activity was significantly reduced<br />
by 0.5 μg/ml <strong>of</strong> cucurbitacin B when compared to the control group (9.83 ± 0.95 and 40.63 ± 2.93, P = 0.0033) or compared<br />
with Phytohemagglutinin (PHA) stimulation (9.83 ± 0.95 and 70.09 ± 3.35, P < 0.0001). A similar pattern <strong>of</strong> CTL activity was<br />
found after 48 and 72 h <strong>of</strong> incubation. In conclusion, cucurbitacin B inhibited PBMC activation by PHA. This study provides<br />
evidences that cucurbitacin B could be used as immunosuppressive agent. © <strong>2010</strong> Academic Journals.<br />
No.1584<br />
Author (s) : Utispan, K., Thuwajit, P., Abiko, Y., Charngkaew, K., Paupairoj, A., Chau-in, S., Thuwajit, C.<br />
Title : Gene expression pr<strong>of</strong>iling <strong>of</strong> cholangiocarcinoma-derived fibroblast reveals alterations related to tumor progression and<br />
indicates periostin as a poor prognostic marker<br />
Source : Molecular Cancer, Volume 9, 24 January <strong>2010</strong>, Article number 13<br />
Document Type : Article<br />
Keywords : alpha5 integrin, amphiregulin, angiopoietin, arylacetamide deacetylase, bone morphogenetic protein 2, bradykinin B1<br />
receptor, calvasculin, enzyme inhibitor, epiregulin, gene product, interleukin 24, Jagged1, laminin alpha 5, macrophage<br />
elastase, matrix remodeling associated 5, membrane protein, metalloproteinase, nephroblastoma overexpressed<br />
protein, platelet derived growth factor A, procollagen C endopeptidase enhancer 2, reelin, response gene to complement<br />
32, secretogranin II, serpin peptidase inhibitor, transcription factor RUNX2, unclassified drug, cell adhesion molecule, POSTN<br />
protein, human, tumor marker; adult, article, bile duct carcinoma, cancer cell, cell invasion, cell proliferation, controlled<br />
study, DNA microarray, female, fibroblast, gene expression, gene expression pr<strong>of</strong>iling, gene expression<br />
regulation, human, human cell, immunohistochemistry, in vitro study, liver cell carcinoma, liver parenchyma, lymph node<br />
metastasis, male, nucleotide sequence, prognosis, protein function, real time polymerase chain reaction, RNA<br />
interference, survival time, tumor growth, tumor promotion, unindexed sequence, Western blotting, bile duct tumor, cancer<br />
invasion, disease course, gene silencing, genetics, intrahepatic bile duct, liver, liver<br />
tumor, metabolism, pathology, proportional hazards model, reproducibility, reverse transcription polymerase chain<br />
reaction, tumor cell line; Bile Duct Neoplasms, Bile Ducts, Intrahepatic, Carcinoma, Hepatocellular, Cell Adhesion<br />
Molecules, Cell Line, Tumor, Cell Proliferation, Cholangiocarcinoma, Disease Progression, Fibroblasts, Gene Expression<br />
Pr<strong>of</strong>iling, Gene Expression Regulation, Neoplastic, Gene Knockdown Techniques, Humans, Immunohistochemistry, Integrin<br />
alpha5, Liver, Liver Neoplasms, Neoplasm Invasiveness, Prognosis, Proportional Hazards Models, Reproducibility <strong>of</strong><br />
Results, Reverse Transcriptase Polymerase Chain Reaction, Tumor Markers, Biological<br />
<strong>Abstract</strong> : Background: Fibroblasts play important roles in several cancers. It was hypothesized that cholangiocarcinoma (CCA)-associated<br />
fibroblasts (Cfs) differ from non-tumorigenic liver fibroblasts (Lfs) in their gene expression pr<strong>of</strong>iles resulting in the capability to<br />
promote cancer. Periostin (PN) is a multi-functional protein and has emerged as a promising marker for tumor progression.<br />
The role <strong>of</strong> PN in CCA, however, has not yet been explored.Results: In this study, the gene expression pr<strong>of</strong>ile <strong>of</strong> Cfs in<br />
comparison to Lfs was performed using oligonucleotide microarrays. The common- and unique-expressed genes in Cfs and<br />
the promising roles in cancer promotion and progression were determined. PN was markedly over-expressed in Cfs confirmed<br />
by real time RT-PCR and western blot analysis. Immunohistochemistry examination <strong>of</strong> a number <strong>of</strong> patients with intrahepatic<br />
CCA showed the expression <strong>of</strong> PN solely in stromal fibroblasts, but was expressed neither in cancer cells nor immune cells.<br />
Low to no expression <strong>of</strong> PN was observed in tissues <strong>of</strong> benign liver disease and hepatocellular carcinoma. CCA patients with<br />
high levels <strong>of</strong> PN had significantly shorter survival time than those with low levels (P = 0.026). Multivariate analysis revealed<br />
high levels <strong>of</strong> PN (P = 0.045) and presence <strong>of</strong> lymph node metastasis (P = 0.002) as independent poor prognostic factors. The<br />
in vitro study revealed that recombinant PN induced CCA cell proliferation and invasion. Interestingly, interference RNA<br />
against integrin α5significantly reduced the cellular response to PN-stimulated proliferation and invasion.Conclusion: The<br />
gene expression pr<strong>of</strong>ile <strong>of</strong> fibroblasts in CCA is apparently explored for the first time and has determined the genes involving<br />
576
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
in induction <strong>of</strong> this cancer progression. High PN can be used to distinguish CCA from other related liver diseases and is<br />
proposed as a prognostic factor <strong>of</strong> poor survival. Regulation <strong>of</strong> fibroblast-derived PN in CCA proliferation and invasion may be<br />
considered as an alternative therapeutic approach. © <strong>2010</strong> Utispan et al; licensee BioMed Central Ltd.<br />
No.1585<br />
Author (s) : Utriyaprasit, K., Moore, S.M., Chaiseri, P.<br />
Title : Recovery after coronary artery bypass surgery: Effect <strong>of</strong> an audiotape information programme<br />
Source : Journal <strong>of</strong> Advanced Nursing, Volume 66, Issue 8, August <strong>2010</strong>, Pages 1747-1759<br />
Document Type : Article<br />
Keywords : Audiotaped information programme; Coronary artery bypass surgery; Nursing; Thailand<br />
<strong>Abstract</strong> : Aim.: The aim <strong>of</strong> the study was to test the effect <strong>of</strong> an audiotape giving concrete objective information and strategies to<br />
reduce symptoms, psychological distress and enhance physical functioning in patients having coronary artery bypass grafts.<br />
Background.: The period following hospital discharge is stressful for patients having coronary artery bypass grafts. Evidentbased<br />
interventions are needed to improve outcomes in Thai populations following coronary artery bypass graft surgery.<br />
Methods.: A randomized controlled trial was conducted during 2004-2005. A sample <strong>of</strong> 120 Thai patients having coronary<br />
artery bypass grafts was randomly assigned to an intervention group or a control group. The intervention group was given an<br />
information audiotape the day prior to hospital discharge, and encouraged to listen to it as many times as necessary.<br />
Participants were interviewed using validated instruments predischarge and at 2 weeks and 4 weeks after discharge. Findings.:<br />
Participants in the intervention group had statistically significantly fewer symptoms <strong>of</strong> shoulder, back or neck pain and lack <strong>of</strong><br />
appetite, and increased physical activity after discharge, compared to the control group. This effect remained statistically<br />
significant after controlling for age, gender, co-morbidity and presurgical cardiac functional status. However, no statistically<br />
significant difference in psychological distress was observed. Conclusion.: Nurses can use an audiotape containing preparatory<br />
information to improve outcomes for patients having coronary artery bypass grafts during the few weeks after discharge from<br />
hospital. Further studies are recommended to improve its effect on psychological distress. © <strong>2010</strong> Blackwell Publishing Ltd.<br />
No.1586<br />
Author (s) : Uttayamakul, S., Likanonsakul, S., Manosuthi, W., Wichukchinda, N., Kalambaheti, T., Nakayama, E.E., Shioda, T., Khusmith, S.<br />
Title : Effects <strong>of</strong> CYP2B6 G516T polymorphisms on plasma efavirenz and nevirapine levels when co-administered with rifampicin in<br />
HIV/TB co-infected Thai adults<br />
Source : AIDS Research and Therapy, Volume 7, 26 March <strong>2010</strong>, Article number 8<br />
Document Type : Article<br />
Keywords : cytochrome P450 2B6, cytochrome P450 3A4, efavirenz, nevirapine, rifampicin, virus RNA; adult, antiviral<br />
therapy, article, bedtime dosage, clinical trial, combination chemotherapy, controlled clinical trial, controlled study, drug<br />
blood level, drug efficacy, female, gene frequency, genetic polymorphism, genotype, human, Human immunodeficiency virus<br />
1, Human immunodeficiency virus 1 infection, major clinical study, male, mixed infection, priority journal, randomized<br />
controlled trial, Thailand, trend study, tuberculosis<br />
<strong>Abstract</strong> : Background: Cytochrome P450 2B6 (CYP2B6) metabolizes efavirenz and nevirapine, the major core antiretroviral drugs for HIV<br />
in Thailand. Rifampicin, a critical component <strong>of</strong> tuberculosis (TB) therapy is a potent inducer <strong>of</strong> CYP enzyme activity.<br />
Polymorphisms <strong>of</strong> CYP2B6 and CYP3A4 are associated with altered activity <strong>of</strong> hepatic enzyme in the liver and<br />
pharmacokinetics resulting in treatment efficacy. This study aimed to investigate whether CYP2B6 or CYP3A4 polymorphisms<br />
had effects on plasma efavirenz and nevirapine concentrations when co-administered with rifampicin in HIV/TB co-infected<br />
Thai adults.Results: We studied 124 rifampicin recipients with concurrent HIV-1/TB coinfection, receiving efavirenz (600<br />
mg/day) (n = 65) or nevirapine (400 mg/day) (n = 59) based antiretroviral therapy (ART). The frequencies <strong>of</strong> GG, GT and TT<br />
genotypes <strong>of</strong> CYP2B6-G516T were 38.46%, 47.69% and 13.85% in efavirenz group and 44.07%, 52.54% and 3.39% in<br />
nevirapine group, respectively. The mean 12-hour post-dose plasma efavirenz concentration in patients with TT genotype at<br />
weeks 6 and 12 <strong>of</strong> ART and 1 month after rifampicin discontinuation (10.97 ± 2.32, 13.62 ± 4.21 and 8.48 ± 1.30 mg/L,<br />
respectively) were significantly higher than those with GT (3.43 ± 0.29, 3.35 ± 0.27 and 3.21 ± 0.22 mg/L, respectively) (p <<br />
0.0001) or GG genotypes (2.88 ± 0.33, 2.45 ± 0.26 and 2.08 ± 0.16 mg/L, respectively) (p < 0.0001). Likewise, the mean 12-hour<br />
post-dose plasma nevirapine concentration in patients carrying TT genotype at weeks 6 and 12 <strong>of</strong> ART and 1 month after<br />
rifampicin discontinuation (14.09 ± 9.49, 7.94 ± 2.76 and 9.44 ± 0.17 mg/L, respectively) tended to be higher than those<br />
carrying GT (5.65 ± 0.54, 5.58 ± 0.48 and 7.03 ± 0.64 mg/L, respectively) or GG genotypes (5.42 ± 0.48, 5.34 ± 0.50 and 6.43 ±<br />
0.64 mg/L, respectively) (p = 0.003, p = 0.409 and p = 0.448, respectively). Compared with the effects <strong>of</strong> CYP2B6-516TT<br />
577
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
genotype, we could observe only small effects <strong>of</strong> rifampicin on plasma efavirenz and nevirapine levels. After 12 weeks <strong>of</strong><br />
both drug regimens, there was a trend towards higher percentage <strong>of</strong> patients with CYP2B6-TT genotype who achieved HIV-1<br />
RNA levels
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
topography, it is as accurate as FE but its speed and memory usage are slightly larger than FD. In addition, we describe a<br />
scheme for automatic mesh generation. This scheme has led to another variant method, a mixed grid hybrid method. Our<br />
hybrid scheme and the automatic grid generation can be used for slopes <strong>of</strong> up to 75° This hybrid method can be further<br />
extended and implemented for 3D DC resistivity modeling for more efficient computation. © <strong>2010</strong> Elsevier B.V.<br />
No.1590<br />
Author (s) : Valecha, N., Looareesuwan, S., Martensson, A., Abdulla, S.M., Krudsood, S., Tangpukdee, N., Mohanty, S., Mishra, S.K., Tyagi,<br />
P.K., Sharma, S.K., Moehrle, J., Gautam, A., Roy, A., Paliwal, J.K., Kothari, M., Saha, N., Dash, A.P., Björkman, A.<br />
Title : Arterolane, a new synthetic trioxolane for treatment <strong>of</strong> uncomplicated Plasmodium falciparum malaria: A phase II,<br />
multicenter, randomized, dose-finding clinical trial<br />
Source : Clinical Infectious Diseases, Volume 51, Issue 6, 15 September <strong>2010</strong>, Pages 684-691<br />
Document Type : Article<br />
Keywords : alanine aminotransferase, arterolane maleate, aspartate aminotransferase, dispiro[adamantane 2,3' [1,2,4]trioxolane 5',4''<br />
cyclohexaneacetic acid 2 amino 2 methylpropylamide], hemoglobin, paracetamol, unclassified drug; abdominal<br />
pain, adolescent, adult, aged, anemia, area under the curve, article, clinical trial, controlled clinical trial, controlled<br />
study, diarrhea, double blind procedure, drug blood level, drug dose comparison, drug efficacy, drug fatality, drug induced<br />
headache, drug safety, female, follow up, human, India, major clinical study, malaria falciparum, male, multicenter<br />
study, phase 2 clinical trial, polymerase chain reaction, priority journal, QT interval, QT prolongation, randomized controlled<br />
trial, side effect, Tanzania, Thailand, treatment outcome, treatment response, vertigo, vomiting;<br />
Adolescent, Adult, Aged, Antimalarials, Double-Blind Method, Female, Heterocyclic Compounds, 1-<br />
Ring, Humans, India, Malaria, Falciparum, Male, Middle Aged, Peroxides, Plasmodium falciparum, Spiro<br />
Compounds, Tanzania, Thailand, Treatment Outcome, Young Adult<br />
<strong>Abstract</strong> : Background: Drug-resistant Plasmodium falciparum malaria necessitates development <strong>of</strong> novel drugs for treatment. The<br />
present study assessed the efficacy and safety <strong>of</strong> 3 dose levels <strong>of</strong> arterolane (RBx 11160), a synthetic trioxolane, for treatment<br />
<strong>of</strong> acute uncomplicated falciparum malaria. Methods: In this randomized, double-blind, multicenter, parallel-group, dosefinding,<br />
phase II trial, 230 patients from 4 centers in Thailand, India, and Tanzania (mainland and Zanzibar) received either 50<br />
mg (n=78), 100 mg (n=76), or 200 mg (n=76) <strong>of</strong> arterolane once daily for 7 days. Patients (aged 13-65 years) with asexual<br />
parasite density <strong>of</strong> 1000-100,000 parasites/μL were included and were followed up for 28 days. The median time to 90%<br />
parasite clearance (PC 90) was evaluated. Results: The median PC90 was longer in the group receiving the 50-mg dose (19.4<br />
h), compared with the groups receiving the 100-mg dose (12.8 h) and 200-mg dose (12.6 h) (P < .01). The polymerase chain<br />
reaction- corrected adequate clinical and parasitological responses on day 28 were 63%, 71%, and 72% for the groups<br />
receiving the 50-mg, 100-mg, and 200-mg doses, respectively, by intention-to-treat analysis (odds ratio, 1.55; 95% confidence<br />
interval, 0.78-3.06, for comparison <strong>of</strong> the 200-mg and 50-mg dose groups). Treatment was generally well tolerated. No patient<br />
died or experienced any serious adverse event. Mild complaints were reported in <10% <strong>of</strong> the patients and were similar in<br />
the 3 groups. Biochemistry and hematological analyses did not show any sign <strong>of</strong> drug toxicity in any patient. Conclusion:<br />
Arterolane at daily doses <strong>of</strong> 100 and 200 mg is a rapidly acting, effective, and safe synthetic antimalarial drug, which may<br />
potentially represent an alternative to artemisinin derivatives in antimalarial combination therapy. © <strong>2010</strong> by the Infectious<br />
Diseases Society <strong>of</strong> America. All rights reserved.<br />
No.1591<br />
Author (s) : Valecha, N., Phyo, A.P., Mayxay, M., Newton, P.N., Krudsood, S., Keomany, S., Khanthavong, M., Pongvongsa, T.,<br />
Ruangveerayuth, R., Uthaisil, C., Ubben, D., Duparc, S., Bacchieri, A., Corsi, M., Rao, B.H.K., Bhattacharya, P.C., Dubhashi, N.,<br />
Ghosh, S.K., Dev, V., Kumar, A., Pukittayakamee, S.<br />
Title : An open-label, randomised study <strong>of</strong> dihydroartemisinin-piperaquine versus artesunate-mefloquine for falciparum malaria in<br />
Asia<br />
Source : PLoS ONE, Volume 5, Issue 7, <strong>2010</strong>, Article number e11880<br />
Document Type : Article<br />
Keywords : artesunate, dihydroartemisinin plus piperaquine, eurartesim, mefloquine, unclassified drug, antimalarial agent, artemisinin<br />
derivative, dihydroquinghaosu, piperaquine, quinoline derivative; abdominal<br />
pain, adolescent, adult, aged, anemia, anorexia, arthralgia, article, Asian, asthenia, child, clinical trial, combination<br />
chemotherapy, controlled clinical trial, controlled study, coughing, dizziness, drug efficacy, drug safety, drug tolerability, drug<br />
treatment failure, eosinophilia, female, fever, follow up, gametocyte, genotype, headache, hemoglobin blood<br />
579
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
level, human, India, infant, Laos, major clinical study, malaria, malaria falciparum, male, multicenter<br />
study, myalgia, nausea, open study, parasite identification, phase 3 clinical trial, polymerase chain reaction, preschool<br />
child, pruritus, QT prolongation, randomized controlled trial, school child, sex difference, skin<br />
allergy, Thailand, thrombocytopenia, vomiting, Asia, middle aged; Artesunate, Plasmodium falciparum;<br />
Adolescent, Adult, Aged, Antimalarials, Artemisinins, Asia, Child, Child, Preschool, Female, Humans, Infant, Malaria,<br />
Falciparum, Male, Mefloquine, Middle Aged, Quinolines, Young Adult<br />
<strong>Abstract</strong> : Background: The artemisinin-based combination treatment (ACT) <strong>of</strong> dihydroartemisinin (DHA) and piperaquine (PQP) is a<br />
promising novel anti-malarial drug effective against multi-drug resistant falciparum malaria. The aim <strong>of</strong> this study was to show<br />
non-inferiority <strong>of</strong> DHA/PQP vs. artesunate-mefloquine (AS+MQ) in Asia. Methods and Findings: This was an open-label,<br />
randomised, non-inferiority, 63-day follow-up study conducted in Thailand, Laos and India. Patients aged 3 months to 65<br />
years with Plasmodium falciparum mono-infection or mixed infection were randomised with an allocation ratio <strong>of</strong> 2:1 to a<br />
fixed-dose DHA/PQP combination tablet (adults: 40 mg/160 mg; children: 20 mg/320 mg; n = 769) or loose combination <strong>of</strong><br />
AS+MQ (AS: 50 mg, MQ: 250 mg; n = 381). The cumulative doses <strong>of</strong> study treatment over the 3 days were <strong>of</strong> about 6.75<br />
mg/kg <strong>of</strong> DHA and 54 mg/kg <strong>of</strong> PQP and about 12 mg/kg <strong>of</strong> AS and 25 mg/kg <strong>of</strong> MQ. Doses were rounded up to the nearest<br />
half tablet. The primary endpoint was day-63 polymerase chain reaction (PCR) genotype-corrected cure rate. Results were<br />
87.9% for DHA/PQP and 86.6% for AS+MQ in the intention-to-treat (ITT;97.5% one sided confidence interval, CI: >-2.87%), and<br />
98.7% and 97.0%, respectively, in the per protocol population (97.5% CI: >-0.39%). No country effect was observed. Kaplan-<br />
Meier estimates <strong>of</strong> proportions <strong>of</strong> patients with new infections on day 63 (secondary endpoint) were significantly lower for<br />
DHA/PQP than AS+MQ: 22.7% versus 30.3% (p = 0.0042; ITT). Overall gametocyte prevalence (days 7 to 63; secondary<br />
endpoint), measured as person-gametocyte-weeks, was significantly higher for DHA/PQP than AS+MQ (10.15% versus 4.88%; p<br />
= 0.003; ITT). Fifteen serious adverse events were reported, 12 (1.6%) in DHA/ PQP and three (0.8%) in AS+MQ, among which<br />
six (0.8%) were considered related to DHA/PQP and three (0.8%) to AS+MQ. Conclusions: DHA/PQP was a highly efficacious<br />
drug for P. falciparum malaria in areas where multi drug parasites are prevalent. The DHA/PQP combination can play an<br />
important role in the first-line treatment <strong>of</strong> uncomplicated falciparum malaria. Trial Registration: Controlled-Trials.com<br />
ISRCTN81306618. © <strong>2010</strong> Valecha et al.<br />
No.1592<br />
Author (s) : Vallé, J., Thaojaikong, T., Moore, C.E., Phetsouvanh, R., Richards, A.L., Souris, M., Fournet, F., Salem, G., Gonzalez, J.J., Newton,<br />
P.N.<br />
Title : Contrasting spatial distribution and risk factors for past infection with scrub typhus and murine typhus in Vientiane city, Lao<br />
PDR<br />
Source : PLoS Neglected Tropical Diseases, Volume 4, Issue 12, December <strong>2010</strong>, Article number e909, Pages 1-10<br />
Document Type : Article<br />
Keywords : immunoglobulin G antibody; accuracy, adult, aged, agricultural worker, article, bacterial transmission, controlled<br />
study, enzyme linked immunosorbent assay, family size, female, geographic distribution, human, Laos, major clinical<br />
study, male, murine typhus, nonhuman, Orientia tsutsugamushi, Rickettsia typhi, risk factor, sanitation, scrub<br />
typhus, seroprevalence, typhus, urban population<br />
<strong>Abstract</strong> : Background: The aetiological diagnostic <strong>of</strong> fevers in Laos remains difficult due to limited laboratory diagnostic facilities.<br />
However, it has recently become apparent that both scrub and murine typhus are common causes <strong>of</strong> previous undiagnosed<br />
fever. Epidemiological data suggests that scrub typhus would be more common in rural areas and murine typhus in urban<br />
areas, but there is very little recent information on factors involved in scrub and murine typhus transmission, especially<br />
where they are sympatric - as is the case in Vientiane, the capital <strong>of</strong> the Lao PDR. Methodology and Principal Findings:We<br />
therefore determined the frequency <strong>of</strong> IgG seropositivity against scrub typhus (Orientia tsutsugamushi) and murine typhus<br />
(Rickettsia typhi), as indices <strong>of</strong> prior exposure to these pathogens, in randomly selected adults in urban and peri-urban<br />
Vientiane City (n=2,002, $35 years). Anti-scrub and murine typhus IgG were detected by ELISA assays using filter paper elutes.<br />
We validated the accuracy <strong>of</strong> ELISA <strong>of</strong> these elutes against ELISA using serum samples. The overall prevalence <strong>of</strong> scrub and<br />
murine typhus IgG antibodies was 20.3% and 20.6%, respectively. Scrub typhus seropositivity was significantly higher among<br />
adults living in the periphery (28.4%) than in the central zone (13.1%) <strong>of</strong> Vientiane. In contrast, seroprevalence <strong>of</strong> murine<br />
typhus IgG antibodies was significantly higher in the central zone (30.8%) as compared to the periphery (14.4%). In<br />
multivariate analysis, adults with a longer residence in Vientiane were at significant greater risk <strong>of</strong> past infection with murine<br />
typhus and at lower risk for scrub typhus. Those with no education, living on low incomes, living on plots <strong>of</strong> land with poor<br />
sanitary conditions, living in large households, and farmers were at higher risk <strong>of</strong> scrub typhus and those living in<br />
580
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
neighborhoods with high building density and close to markets were at greater risk for murine typhus and at lower risk <strong>of</strong><br />
scrub typhus past infection. Conclusions: This study underscores the intense circulation <strong>of</strong> both scrub and murine typhus in<br />
Vientiane city and underlines difference in spatial distribution and risk factors involved in the transmission <strong>of</strong> these diseases.<br />
No.1593<br />
Author (s) : van der Windt, G.J.W., Wiersinga, W.J., Wieland, C.W., Tjia, I.C.S.I., Day, N.P., Peacock, S.J., Florquin, S., van der Poll, T.<br />
Title : Osteopontin impairs host defense during established gram-negative sepsis caused by Burkholderia pseudomallei(Melioidosis)<br />
Source : PLoS Neglected Tropical Diseases, Volume 4, Issue 8, August <strong>2010</strong>, Article number e806<br />
Document Type : Article<br />
Keywords : cytokine, osteopontin; animal cell, animal experiment, animal model, animal tissue, article, bacterial count, Burkholderia<br />
pseudomallei, clinical article, controlled study, disease severity, Gram negative sepsis, host pathogen<br />
interaction, human, knockout mouse, lung injury, male, melioidosis, mortality, mouse, neutrophil<br />
chemotaxis, nonhuman, protein blood level, protein function, protein synthesis, tissue necrosis, wild type;<br />
Animals, Burkholderia pseudomallei, Colony Count, Microbial, Humans, Lung, Melioidosis, Mice, Mice, Inbred C57BL, Mice,<br />
Knockout, Necrosis, Neutrophils, Osteopontin<br />
<strong>Abstract</strong> : Background: Melioidosis, caused by infection with Burkholderia (B.) pseudomallei, is a severe illness that is endemic in<br />
Southeast Asia. Osteopontin (OPN) is a phosphorylated glycoprotein that is involved in several immune responses including<br />
induction <strong>of</strong> T-helper 1 cytokines and recruitment <strong>of</strong> inflammatory cells. Methodology and Principal Findings:OPN levels were<br />
determined in plasma from 33 melioidosis patients and 31 healthy controls, and in wild-type (WT) mice intranasally infected<br />
with B. pseudomallei. OPN function was studied in experimental murine melioidosis using WT and OPN knockout (KO) mice.<br />
Plasma OPN levels were elevated in patients with severe melioidosis, even more so in patients who went on to die. In<br />
patients who recovered plasma OPN concentrations had decreased after treatment. In experimental melioidosis in mice<br />
plasma and pulmonary OPN levels were also increased. Whereas WT and OPN KO mice were indistinguishable during the first<br />
24 hours after infection, after 72 hours OPN KO mice demonstrated reduced bacterial numbers in their lungs, diminished<br />
pulmonary tissue injury, especially due to less necrosis, and decreased neutrophil infiltration. Moreover, OPN KO mice<br />
displayed a delayed mortality as compared to WT mice. OPN deficiency did not influence the induction <strong>of</strong> proinflammatory<br />
cytokines. Conclusions: These data suggest that sustained production <strong>of</strong> OPN impairs host defense during established septic<br />
melioidosis. © <strong>2010</strong> van der Windt et al.<br />
No.1594<br />
Author (s) : Van Panhuis, Willem G.; Luxemburger, Christine; Pengsaa, Krisana; Limkittikul, Kriengsak; Sabchareon, Arunee; Cummings, Derek<br />
A.; Lang, Jean; Durbin, Anna P.<br />
Title : A LONGITUDINAL ANALYSIS OF MATERNAL DENGUE ANTIBODY KINETICS AMONG INFANTS IN BANGKOK<br />
Source : AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, Volume: 83, Issue: 5, Supplement: S, Pages: 141-141, Meeting<br />
<strong>Abstract</strong>: 470, Published: NOV <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1595<br />
Author (s) : Vanavanan, S., Santanirand, P., Chaichanajarernkula, U., Chittamma, A., DuBois, J.A., Shirey, T., Heinz, M.<br />
Title : Performance <strong>of</strong> a new interference-resistant glucose meter<br />
Source : CLINICAL BIOCHEMISTRY, Volume: 43, Issue: 1-2, Pages: 186-192, DOI: 10.1016/j.clinbiochem.2009.09.010, Published: JAN <strong>2010</strong><br />
Document Type : Article<br />
Keywords : Glucose meter; Interference-resistant glucose meter; POCT; Point-<strong>of</strong>-Care-Testing; Self-monitoring <strong>of</strong> blood glucose (SMBG)<br />
<strong>Abstract</strong> : Objectives: Glucose meters are widely used in self and hospital monitoring <strong>of</strong> blood glucose. We examined the analytical<br />
performance <strong>of</strong> a StatStrip glucose monitoring system. Design and methods: Linearity, % recovery and within-run imprecision<br />
were studied using glucose-spiked whole blood. A total <strong>of</strong> 120 heparinized samples were used in method comparison using a<br />
plasma hexokinase on the Dimension RxL MAX analyzer as the comparison method. Common interferences were tested on<br />
the StatStrip, Accu-Chek Advantage and the MediSense Optium glucose meters at low, middle and high glucose levels.<br />
Results: The StatStrip assay showed excellent linearity and recovery. The coefficient <strong>of</strong> variations for imprecision were
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
the three meters tested, only the StatStrip showed interference
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
admitted to the Hospital for Tropical Diseases, Bangkok, Thailand. Nine hundred fifteen severe malaria patients were included<br />
in the study, <strong>of</strong> whom 195 had ARF and 720 did not have ARF. We found jaundice, anemia, hypoalbuminemia, hyponatremia,<br />
hyperkalemia, acidosis, leukocytosis, elevated transaminases (SGOT and SGPT) and cerebral malaria, were significantly<br />
associated with ARF among patients with severe malaria (p
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
0.60 (95% CI, 0.35-1.03), and 1.87 (95% CI, 0.77-4.56), respectively, for men, and were 1.29 (95% CI,1.04-1.60), 0.70 (95% CI,<br />
0.55-0.90), 0.79 (95% CI, 0.62-1.01), 0.57 (95% CI, 0.41-0.81), 0.58 (95% CI, 0.39-0.87), and 0.78 (95% CI, 0.38-1.59), respectively,<br />
for women. Conclusions: The results <strong>of</strong> this study support the obesity paradox phenomenon in older Thai people, especially<br />
in women. Improvement in quality <strong>of</strong> mortality data and further investigation to confirm such association are needed in this<br />
population. © <strong>2010</strong> Vapattanawong et al; licensee BioMed Central Ltd.<br />
No.1601<br />
Author (s) : Vasuvattakul, S.<br />
Title : Molecular approach for distal renal tubular acidosis associated AE1 mutations<br />
Source : Electrolyte and Blood Pressure, Volume 8, Issue 1, June <strong>2010</strong>, Pages 25-31<br />
Document Type : Review<br />
Keywords : Acidosis; Anion exchange protein 1; Chloride-bicarbonate antiporters; Compound heterozygosity; Erythrocyte; Renal<br />
tubular; Southeast Asian ovalocytosis; Trafficking defect; Urine-blood PCO2<br />
<strong>Abstract</strong> : The molecular approaches to distal renal tubular acidosis (dRTA) associated AE1 mutations lead us to understand the genetic<br />
and pathophysiological aspects <strong>of</strong> the acidification defects. An unanticipated high value <strong>of</strong> the urine-blood (U-B) PCO2 after<br />
NaHCO3 loading observed in a case <strong>of</strong> dRTA and southeast Asian ovalocytosis (SAO) might be from a mistarget <strong>of</strong> the AE1 to<br />
the luminal membrane <strong>of</strong> type A intercalated cells. The mutations <strong>of</strong> the AE1 gene resulted in SAO and also affected renal<br />
acidification function. Notwithstanding, after the NH4CI loading in 20 individuals with SAO, the acidification in the distal<br />
nephron was normal. The presence <strong>of</strong> both SAO and G701D mutations <strong>of</strong> AE1 gene would explain the abnormal urinary<br />
acidification in the patients with the compound heterozogosity. In terms <strong>of</strong> the effect <strong>of</strong> the mutations on trafficking <strong>of</strong> AE1,<br />
truncated kidney is<strong>of</strong>orm (kAE1) <strong>of</strong> wild-type showed a 'dominant-positive effect' in rescuing the recessive mutant kAE1 (S773P<br />
or G701D) trafficking to the plasma membrane, in contrast with the dominant mutant kAE1 (R589H) resulting in a 'dominantnegative<br />
effect' when heterodimerized with the wild-type kAE1. It is notable that the dominant mutants kAE1 (R901X or<br />
G609R) expression in MDCK cells clearly results in aberrant surface expression with some mutant protein appearing at the<br />
apical membrane. These might result in net bicarbonate secretion and increasing U-B PCO 2 in the distal nephron. The<br />
molecular physiological and genetic approaches have permitted identification <strong>of</strong> the molecular defects, predominantly in<br />
transporter proteins, and should in turn prompt development <strong>of</strong> novel therapeutic strategies. Copyright © <strong>2010</strong> The Korean<br />
Society <strong>of</strong> Electrolyte Metabolism.<br />
No.1602<br />
Author (s) : Vatananan, R.S., Gerdsri, N.<br />
Title : The current state <strong>of</strong> technology roadmapping (TRM) research and practice<br />
Source : PICMET '10 - Portland <strong>International</strong> Center for Management <strong>of</strong> Engineering and Technology, Proceedings - Technology<br />
Management for Global Economic Growth, <strong>2010</strong>, Article number 5603435, Pages 311-320<br />
Document Type : Conference Paper<br />
Keywords : Business strategy, Gaining momentum, Roadmap, Roadmapping, Strategic management tools, Technology roadmapping;<br />
Economics, Industrial management, Research, Strategic planning; Technology<br />
<strong>Abstract</strong> : Technology roadmapping (TRM) is gaining momentum as a strategic management tool for an organization to integrate<br />
technology into business strategy. This paper reviews over a hundred <strong>of</strong> TRM related publications to provide the community<br />
with the current state <strong>of</strong> roadmapping research and practice. From the literature, the issue <strong>of</strong> keeping a roadmap alive has<br />
been identified as the key challenge in the field. To address this challenge, operationalization and effective implementation<br />
<strong>of</strong> TRM are highlighted as the potential areas for future research. © <strong>2010</strong> IEEE.<br />
No.1603<br />
Author (s) : Vatanavicharn, N., Visitsunthorn, N., Pho-Iam, T., Jirapongsananuruk, O., Pacharn, P., Chokephaibulkit, K., Limwongse, C.,<br />
Wasant, P.<br />
Title : An infant with cartilage-hair hypoplasia due to a novel homozygous mutation in the promoter region <strong>of</strong> the RMRP gene<br />
associated with chondrodysplasia and severe immunodeficiency<br />
Source : Journal <strong>of</strong> Applied Genetics, Volume 51, Issue 4, <strong>2010</strong>, Pages 523-528<br />
Document Type : Article<br />
Keywords : Cartilage-hair hypoplasia; Combined immune deficiency; Promoter region mutation; RMRP gene<br />
584
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : Cartilage-hair hypoplasia (CHH) is a rare autosomal-recessive disorder characterized by short-limbed dwarfism, sparse hair, and<br />
immune deficiency. It is caused by mutations in the RMRP gene, which encodes the RNA component <strong>of</strong> the mitochondrial<br />
RNA-processing ribonuclease (RNase MRP). Several mutations have been identified in its promoter region or transcribed<br />
sequence. However, homozygous mutations in the promoter region have been only reported in a patient with primary<br />
immunodeficiency without other features <strong>of</strong> CHH. We report on a Thai girl who first presented with chronic diarrhea, recurrent<br />
pneumonia, and severe failure to thrive, without apparently disproportionate dwarfism. The diagnosis <strong>of</strong> CHH was made after<br />
the severe wasting was corrected, and disproportionate growth became noticeable. The patient had the typical features <strong>of</strong><br />
CHH, including sparse hair and metaphyseal abnormalities. The immunologic pr<strong>of</strong>iles were consistent with combined immune<br />
deficiency. Mutation analysis identified a novel homozygous mutation, g.-19_-25 dupACTACTC, in the promoter region <strong>of</strong> the<br />
RMRP gene. Identification <strong>of</strong> the mutation enabled us to provide a prenatal diagnosis in the subsequent pregnancy. This<br />
patient is the first CHH case with the characteristic features due to the homozygous mutation in the promoter region <strong>of</strong> the<br />
RMRP gene. The finding <strong>of</strong> severe immunodeficiency supports that promoter mutations markedly disrupt mRNA cleavage<br />
function, which causes cell-cycle impairment.<br />
No.1604<br />
Author (s) : Vatanavicharn, N.; Champattanachai, V; Liammongkolkul, S.; Sawangareetrakul, P.; Keeratichamroen, S.; Cairns, J. R. K.;<br />
Srisomsap, C.; Sathienkijkanchai, A.; Svasti, J.; Wasant, P.<br />
Title : GENETIC ANALYSIS OF MUT, MMAA, AND MMAB IN THAI PATIENTS WITH ISOLATED METHYLMALONIC ACIDEMIA<br />
Source : JOURNAL OF INHERITED METABOLIC DISEASE, Volume: 33, Supplement: 1, Pages: S42-S42, Published: AUG <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1605<br />
Author (s) : Vathanophas, V., Pacharapha, T.<br />
Title : Information technology acceptance in healthcare service: The study <strong>of</strong> Electronic Medical Record (EMR) in Thailand<br />
Source : PICMET '10 - Portland <strong>International</strong> Center for Management <strong>of</strong> Engineering and Technology, Proceedings - Technology<br />
Management for Global Economic Growth, <strong>2010</strong>, Article number 5603355, Pages 2888-2892<br />
Document Type : Conference Paper<br />
Keywords : Data intensive, Data volume, Electronic medical record, Explosive growth, Healthcare services, Hospital information<br />
systems, Information technology acceptance, Medical information, Organizational development, Organizational<br />
infrastructure, Technology acceptance model, Technology usages, Thailand, User acceptance; Economics, Hospitals, Industrial<br />
management, Medical computing; Technology<br />
<strong>Abstract</strong> : The explosive growth in technology usage has put growing pressure on organizations to serve customers electronically.<br />
Healthcare service, as one <strong>of</strong> data intensive industries, tries to serve their patients with excellent service in the timely manner<br />
when the data volume is growing faster than organizational infrastructure development. Because the hospital information<br />
system called Electronic Medical Record (EMR) is a powerful tool, and provides a multitude <strong>of</strong> benefits, many hospitals are<br />
today considering accepting, and adopting this technology to provide medical information and healthcare services in a better<br />
way. This study will extend applicability <strong>of</strong> the Technology Acceptance Model (TAM) to test user acceptance <strong>of</strong> EMR in the<br />
hospital context in Thailand. The study aims to investigate EMR characteristics which affect Electronic Medical Record (EMR)<br />
acceptance in the healthcare pr<strong>of</strong>ession's belief perspective in hospital. This EMR acceptance measurement will help the<br />
organization forecast how healthcare pr<strong>of</strong>essions' perception <strong>of</strong> EMR usage will affect and support the organizational<br />
development. The result can be used as a guideline for EMR implementation that may lead to a successful EMR initiative in<br />
Thailand. © <strong>2010</strong> IEEE.<br />
No.1606<br />
Author (s) : Vathesatogkit, P.; Tunthong, R.; Pathumarak, A.; Angchaisuksiri, P.; Pienvichit, P.; Boonbaichaiyapruck, S.; Sritara, P.<br />
Title : Calcium-channel blockers and the antiplatelet effect <strong>of</strong> clopidogrel: potential misleading evidence from high residual platelet<br />
activity<br />
Source : EUROPEAN HEART JOURNAL, Volume: 31, Supplement: 1, Pages: 968-968, Published: SEP <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
585
<strong>Abstract</strong> : –<br />
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.1607<br />
Author (s) : Vayachuta, L., Phinyocheep, P., Derouet, D., Pascual, S.<br />
Title : Synthesis <strong>of</strong> ATRP macroinitiator based on synthetic cis-1,4-polyisoprene and its application for graft polymerization <strong>of</strong> MMA<br />
Source : E-Polymers, 13 March <strong>2010</strong>, Article number 29<br />
Document Type : Article<br />
Keywords : ATRP macroinitiator; Poly(methyl methacrylate); Polyisoprene<br />
<strong>Abstract</strong> : Modification <strong>of</strong> synthetic cis-1,4-polyisoprene (PI) into bromoalkyl-functionalized polyisoprene (PIBr), an ATRP macroinitiator,<br />
was investigated by two-step chemical reaction. The PI was partially epoxidized into epoxidized polyisoprene (EPI) using mchloroperbenzoic<br />
acid, then the EPI was transformed into PIBr by reaction with 2-bromo-2-methylpropionic acid. The results<br />
from 1H NMR revealed that the addition product occurs in competition with epoxide ring rearrangement. The amount <strong>of</strong><br />
bromoalkyl functionalized units was determined from 1H NMR spectra. The graft polymerization <strong>of</strong> MMA using ATRP<br />
technique from macroinitiator units in PIBr was investigated using three different ligands, i.e. N-(n-octyl)-2- pyridylmethanimine<br />
(NOPMI), N-(n-octadecyl)-2-pyridylmethanimine (NODPMI) and 1,1,4,7,7-pentamethyldiethylenetriamine (PMDETA), resulting in<br />
formation <strong>of</strong> graft copolymer <strong>of</strong> PI and PMMA. The PMMA grafts were successfully separated from the PI backbone using<br />
acidolysis for studying their number-average molecular weights (Mn,SEC) and polydispersity indexes (PDI). All <strong>of</strong> the ligands<br />
used give a increase <strong>of</strong> M̄n with MMA conversion. Comparing between 3 ligands, Cu(I)Br complexed with NOPMI shows firstorder<br />
kinetic plot.<br />
No.1608<br />
Author (s) : Vejbaesya, S., Luangtrakool, P., Luangtrakool, K., Stephens, H.A.<br />
Title : Tumor necrosis factor (TNF) and lymphotoxin (LTA) allele and extended MHC haplotype associations with severe dengue virus<br />
infections in mainland SE Asians<br />
Source : TISSUE ANTIGENS, Volume: 75, Issue: 5, Pages: 617-618, Published: MAY <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1609<br />
Author (s) : Vichaiwong, K., Purohit, S., An, D., Toyoda, T., Jessen, N., Hirshman, M.F., Goodyear, L.J.<br />
Title : Contraction regulates site-specific phosphorylation <strong>of</strong> TBC1D1 in skeletal muscle<br />
Source : Biochemical Journal, Volume 431, Issue 2, 15 October <strong>2010</strong>, Pages 311-320<br />
Document Type : Article<br />
Keywords : Akt; AMP-activated protein kinase (AMPK); Contraction; Glucose transport; Skeletal muscle; TBC1D1<br />
<strong>Abstract</strong> : TBC1D1 (tre-2/USP6, BUB2, cdc16 domain family member 1) is aRab-GAP (GTPase-activating protein) that is highly expressed in<br />
skeletal muscle, but little is known about TBC1D1 regulation and function. We studied TBC1D1 phosphorylation on three<br />
predicted AMPK (AMP-activated protein kinase) phosphorylation sites (Ser231, Ser660 and Ser 700) and one predicted Akt<br />
phosphorylation site (Thr590) in control mice, AMPKα2 inactive transgenic mice (AMPKα2i TG) and Akt2-knockout mice (Akt2<br />
KO). Muscle contraction significantly increased TBC1D1 phosphorylation on Ser231 and Ser660, tended to increase Ser700<br />
phosphorylation, but had no effect on Thr590. AICAR (5-aminoimidazole-4-carboxyamide ribonucleoside) also increased<br />
phosphorylation on Ser231, Ser660 and Ser700, but not Thr 590, whereas insulin only increased Thr590 phosphorylation.<br />
Basal and contraction-stimulated TBC1D1 Ser231, Ser660 and Ser700 phosphorylation were greatly reduced in AMPKα2i TG<br />
mice, although contraction still elicited a small increase in phosphorylation. Akt2 KO mice had blunted insulin-stimulated<br />
TBC1D1 Thr 590 phosphorylation. Contraction-stimulated TBC1D1 Ser231 and Ser660 phosphorylation were normal in high-fatfed<br />
mice. Glucose uptake in vivo was significantly decreased in tibialis anterior muscles overexpressing TBC1D1 mutated on<br />
four predicted AMPK phosphorylation sites. In conclusion, contraction causes site-specific phosphorylation <strong>of</strong> TBC1D1 in<br />
skeletal muscle, and TBC1D1 phosphorylation on AMPK sites regulates contraction-stimulated glucose uptake. AMPK and Akt<br />
regulate TBC1D1 phosphorylation, but there must be additional upstream kinases that mediate TBC1D1 phosphorylation in<br />
skeletal muscle. © <strong>2010</strong> The Author(s).<br />
586
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.1610<br />
Author (s) : Vichyanond, P., Suratannon, C., Lertbunnaphong, P., Jirapongsananuruk, O., Visitsunthorn, N.<br />
Title : Clinical characteristics <strong>of</strong> children with non-allergic rhinitis vs with allergic rhinitis<br />
Source : Asian Pacific Journal <strong>of</strong> Allergy and Immunology, Volume 28, Issue 4, December <strong>2010</strong>, Pages 270-274<br />
Document Type : Article<br />
Keywords : Allergic rhinitis; Children; Non-allergic rhininitis; Skin testing<br />
<strong>Abstract</strong> : Background: Allergic rhinitis (AR) and nonallergic rhinitis (NAR) are major causes <strong>of</strong> chronic rhinitis. Knowledge about children<br />
with non-allergic rhinitis is limited. Objective: To study clinical characteristics differentiating NAR and AR among children with<br />
chronic rhinitis. Methods: This is a retrospective, descriptive study <strong>of</strong> 302 children (with ages <strong>of</strong> 14 years or less) with chronic<br />
rhinitis evaluated at the pediatric allergy clinic, Siriraj Hospital between January and December 2006. Based on the results <strong>of</strong><br />
skin prick test (SPT), they were classified into 2 groups, i.e., AR and NAR. Their medical records were reviewed with respect to<br />
clinical data on rhinitis and related symptoms. Results: There were 222 patients with AR and 80 with NAR (73.5% and 26.5%).<br />
Median age <strong>of</strong> onset <strong>of</strong> the disease among patients with NAR was younger than AR (p = 0.04) while the duration <strong>of</strong> disease<br />
among AR cases was longer than in NAR (p < 0.01). Severity <strong>of</strong> rhinitis, based on Allergic Rhinitis and its Impact on Asthma<br />
(ARIA), was not different between the two groups. Nasal pruritus, sneezing and eye symptoms were more commonly<br />
observed in AR than in NAR (p
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Source : HISTOPATHOLOGY, Volume: 57, Supplement: 1, Pages: 107-108, Meeting <strong>Abstract</strong>: 300, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1614<br />
Author (s) : Viprakasit, V., Gattermann, N., Lee, J.W., Porter, J.B., Taher, A., Habr, D., Roubert, B., Domokos, G., Cappellini, M.D.<br />
Title : Geographical Differences In Transfusion and Iron Chelation Practices In 1558 Patients with Transfusion-Dependent Anemias<br />
Source : BLOOD, Volume: 116, Issue: 21, Pages: 1733-1734, Published: NOV 19 <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1615<br />
Author (s) : Virayavanich, W., Sirikulchayanonta, V., Jaovisidha, S., Hongeng, S., Laohacharoensombat, W., Pornkul, R.<br />
Title : Presacral fibrosarcoma in childhood: A case report<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 2, February <strong>2010</strong>, Pages 252-256<br />
Document Type : Article<br />
Keywords : Childhood fibrosarcoma; Presacral mass; Sacrum<br />
<strong>Abstract</strong> : Presacral fibrosarcoma is a rare malignant tumor in childhood. Because <strong>of</strong> its rarity in the presacral region, it is <strong>of</strong>ten missed<br />
from the differential diagnosis <strong>of</strong> presacral mass in childhood. The authors present a case <strong>of</strong> large presacral fibrosarcoma<br />
involving spinal canal, with an initial presentation <strong>of</strong> back pain and abnormal gaiting.<br />
No.1616<br />
Author (s) : Viriyasaksathian, B., Wongsawat, Y., Suriyaphol, P.<br />
Title : CADViST: Visualization tool for BLAST Alignment <strong>of</strong> Dengue virus sequences<br />
Source : <strong>2010</strong> 4th <strong>International</strong> Conference on Bioinformatics and Biomedical Engineering, iCBBE <strong>2010</strong>, <strong>2010</strong>, Article number 5515779<br />
Document Type : Conference Paper<br />
Keywords : Bioinformatics; BLAST; Dengue virus; Visualization<br />
<strong>Abstract</strong> : Exploration <strong>of</strong> the search engine that can simultaneously visualize the genomic sequences is one <strong>of</strong> the challenging<br />
problems. In this paper, we propose the s<strong>of</strong>tware, called CADViST. The UnitX graphical representation (previously proposed<br />
by the authors) is employed as the alternative tool to visualize the result obtained from the Basic Local Alignment Search<br />
Tool (BLAST). The proposed s<strong>of</strong>tware can efficiently help the users/experts to easily interpret the results, especially in<br />
Dengue virus sequence analysis where different serotypes or subtypes need to be distinguished. © <strong>2010</strong> IEEE.<br />
No.1617<br />
Author (s) : Visavachaipan, N., Ong-Ajyooth, L., Chanchairujuira, T., Parichatikanond, P., Choensuchon, B.<br />
Title : Clinical features and outcomes in patient with antineutrophil cytoplasmic autoantibody-positive glomerulonephritis<br />
associated with propylthiouracil treatment in Siriraj Hospital.<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand = Chotmaihet thangphaet, Volume 93 Suppl 1, January <strong>2010</strong>, Pages S139-146<br />
Document Type : Article<br />
Keywords : antithyroid agent, corticosteroid, neutrophil cytoplasmic antibody, propylthiouracil; adult, aged, article, blood, chemically<br />
induced disorder, female, glomerulonephritis, Graves disease, human, immunology, kidney disease, middle aged, retrospective<br />
study, treatment outcome; Adrenal Cortex Hormones, Adult, Aged, Antibodies, Antineutrophil Cytoplasmic, Antithyroid<br />
Agents, Female, Glomerulonephritis, Graves Disease, Humans, Kidney Diseases, Middle Aged, Propylthiouracil, Retrospective<br />
Studies, Treatment Outcome<br />
<strong>Abstract</strong> : BACKGROUND: ANCA is detected in several vasculitic diseases, including drug-induced systemic vasculitis: propylthiouracil<br />
(PTU), hydralazine, minocycline, penicillamine, allopurinol, procainamide, carbimazole, thiamazole, clozapine and phenytoin.<br />
All have been known to induce ANCA positive vasculitis in adult patients. OBJECTIVE: To study the clinical manifestation,<br />
renal pathology and outcome <strong>of</strong> patients with ANCA positive vasculitis associated with propylthiouracil treatment in Siriraj<br />
Hospital. MATERIAL AND METHOD: Retrospective study in 7patients with Graves' disease who were treated with<br />
propylthiouracil and developed ANCA-positive glomerulonephritis between 2000-2008. RESULTS: Seven cases with Graves'<br />
588
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
disease who received propylthiouracil whose ages were 43 +/- 14 years. The duration <strong>of</strong> propylthiouracil treatment was 68.5<br />
+/- 39 months and the doses were 50-150 mg per day. Six cases had P-ANCA and one case had C-ANCA in the serum.<br />
Proteinuria ranged from 0.49-2.9 gram per day. Mean serum creatinine was 2.05 mg/dl with creatinine clearance <strong>of</strong> 44 +/- 35<br />
ml/min. The propylthiouracil was withdrawn in every patient and corticosteroid was administered. Renal remission was found<br />
until 1 year <strong>of</strong> follow-up. CONCLUSION: ANCA positive glomerulonephritis associated with propylthiouracil is not uncommon.<br />
The average onset <strong>of</strong> glomerulonephritis is 2 years or more. The propylthiouracil dosage was not necessary high. Urinalysis<br />
and other glomerulonephritis symptoms should be screened for early diagnosis and appropriate treatment in patients treated<br />
with PTU.<br />
No.1618<br />
Author (s) : Visitsunthorn, N., Chirdjirapong, V., Pootong, V., Jirapongsananuruk, O., Pacharn, P., Weeravejsukit, S., Mahakittikun, V.,<br />
Vichyanond, P.<br />
Title : The accumulation <strong>of</strong> dust mite allergens on mattresses made <strong>of</strong> different kinds <strong>of</strong> materials<br />
Source : Asian Pacific Journal <strong>of</strong> Allergy and Immunology, Volume 28, Issue 2-3, June <strong>2010</strong>, Pages 155-161<br />
Document Type : Article<br />
Keywords : Der f1; Der p1; Group 1 allergens; Mattress; Mite allergen<br />
<strong>Abstract</strong> : Background: Different mattress materials may affect the accumulation <strong>of</strong> allergens. Objective: To compare the amount <strong>of</strong><br />
group 1 dust mite allergens (Der p1 + Der f1) on mattresses made <strong>of</strong> different kinds <strong>of</strong> materials before and after use.<br />
Methods: Sixty new mattresses made <strong>of</strong> kapok, synthetic fiber, coconut fiber and sponge-like polyurethane, were placed in<br />
the house <strong>of</strong>ficers' dormitory at Siriraj hospital, Thailand. The dust samples were collected before (0), 1, 2, 3, 6, 9 and 12<br />
months after the mattresses were used. Group 1 dust mite allergens were analyzed using two-site monoclonal antibody<br />
ELISA. Results: Der f1 made up 86.7 % <strong>of</strong> group 1 allergens found in the matress dust. After the 2nd month, only the mean<br />
level in sponge-like polyurethane mattress was under 2 μg/g dust (sensitized level). At the 6th month, the mean levels were<br />
13.1 in coconut, 21.7 in kapok and 17.3 μg/g dust in synthetic fiber, all <strong>of</strong> which were more than 10 μg/g dust (symptomatic<br />
level). At the 9th month, the level in sponge-like polyurethane mattress was increased to 11.2 μg/g. At 12th month the level<br />
in coconut fiber, sponge-like polyurethane synthetic fiber and kapok mattresses were 20.2, 22.4, 28.9 and 32.2 μg/g dust<br />
respectively. Conclusions: The accumulation rate in kapok and synthetic mattresses was significantly higher than coconut and<br />
sponge-like polyurethane mattresses. The mean level <strong>of</strong> group 1 mite allergens exceeded 10 μg/g dust after the 6th month<br />
<strong>of</strong> use in coconut fiber, kapok and synthetic fiber and at the 9th month in sponge-like polyurethane mattress.<br />
No.1619<br />
Author (s) : Visitsunthorn, N., Pacharn, P., Jirapongsananuruk, O., Weeravejsukit, S., Sripramong, C., Sookrung, N., Bunnag, C.<br />
Title : Comparison between siriraj mite allergen vaccine and standardized commercial mite vaccine by skin prick testing in normal<br />
thai adults<br />
Source : Asian Pacific Journal <strong>of</strong> Allergy and Immunology, Volume 28, Issue 1, March <strong>2010</strong>, Pages 41-45<br />
Document Type : Article<br />
Keywords : house dust allergen; adult, article, clinical trial, controlled clinical trial, controlled study, Dermatophagoides<br />
farinae, Dermatophagoides pteronyssinus, double blind procedure, drug dosage form comparison, drug<br />
formulation, experimental study, female, human, human experiment, immunoreactivity, male, normal human, prick test, skin<br />
manifestation, skin test; Adolescent, Adult, Antigens, Dermatophagoides, Complex Mixtures, Cost Savings, Double-Blind<br />
Method, Feasibility Studies, Female, Humans, Hypersensitivity, Intradermal Tests, Male, Middle Aged, Reagent Kits,<br />
Diagnostic, Skin, Thailand<br />
<strong>Abstract</strong> : House dust mite is a major cause <strong>of</strong> allergic asthma and rhinitis in Thai population. Skin prick test (SPT) is a useful tool for the<br />
diagnosis <strong>of</strong> the IgE-mediated reactions. The imported commercial mite vaccine for SPT is available but it is relatively<br />
expensive. Aim <strong>of</strong> this studyis to compare Siriraj Mite Allergen Vaccine (SMAV) with standardized commercial mite allergen<br />
vaccine by skin prick testing in normal Thai adults. A double blind, self-controlled study between the SMAV and standardized<br />
commercial mite allergen vaccine was performed by SPT in 17 normal Thai adult males and non-pregnant or non-lactating<br />
females aged 18-60 years. The study showed that 35.29 % <strong>of</strong> non atopic adults had positive SPT reaction to Dp and Df <strong>of</strong><br />
both SMAV andstandardized commercial mite allergen vaccine. Mean wheal and flare diameters from SPT <strong>of</strong> Dp and Df <strong>of</strong><br />
SMAV showed strong correlation with standardized commercial mite allergen vaccine (r = 0.768 and 0.897 in Dp and Df<br />
respectively, p
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
no significant difference in wheal and flare diameter between SMAV andstandardized commercial mite allergen vaccine. No<br />
systemic or large local reaction was found in any <strong>of</strong> the study cases.<br />
No.1620<br />
Author (s) : Visoottiviseth, V., Ngamtura, P.<br />
Title : On the performance <strong>of</strong> MIPv6 and FMIPv6 based on real IPv6 applications over IEEE 802.11g testbeds<br />
Source : ISCIT <strong>2010</strong> - <strong>2010</strong> 10th <strong>International</strong> Symposium on Communications and Information Technologies, <strong>2010</strong>, Article number<br />
5665176, Pages 1217-1222<br />
Document Type : Conference Paper<br />
Keywords : Fast handovers, Hand over, Handover latency, Handover process, IEEE 802.11g, IPv6 networks, LINUX- operating system, Mean<br />
opinion scores, Measurement tools, Mobile IPv6, Mobile nodes, Mobility supports, Network connection, Performance<br />
evaluation, Performance metrics, Performance study, Real applications, Subnets, Wireless test beds; Acoustic<br />
streaming, Computer operating systems, Information technology, Jitter, Numerical analysis, Standards, Test<br />
facilities, Testbeds, Video streaming, Videotex, Wireless networks; Internet protocols<br />
<strong>Abstract</strong> : Mobility support in IPv6 networks (MIPv6) and Fast Handovers for Mobile IPv6 (FMIPv6) are already released as RFCs for many<br />
years. While the former focuses on maintaining the network connections when a mobile node handovers across subnets, the<br />
latter is built based on the former concept, but focuses on reducing the packet loss and latency inherent to the handover<br />
process. Performance study <strong>of</strong> both protocols was conducted, but most <strong>of</strong> them are based on simulations or numerical<br />
analysis. Therefore, in this paper we present a set <strong>of</strong> experiments with real implementations <strong>of</strong> MIPv6 and FMIPv6 for Linux<br />
operating system, and their performance evaluation based on real applications. We create a real IEEE 802.11g wireless<br />
testbed and run real IPv6 applications, such as FTP and the video streaming. Jperf measurement tool is also used to measure<br />
the UDP performance as well. Performance metrics we observe are throughput, jitter, and handover latency. For video<br />
streaming, the Mean Opinion Scores (MOS) is used to evaluate. ©<strong>2010</strong> IEEE.<br />
No.1621<br />
Author (s) : Visoottiviseth, V., Trunganont, A., Siwamogsatham, S.<br />
Title : Adaptive bandwidth allocation for per-station fairness on wireless access router<br />
Source : ISCIT <strong>2010</strong> - <strong>2010</strong> 10th <strong>International</strong> Symposium on Communications and Information Technologies, <strong>2010</strong>, Article number<br />
5664845, Pages 238-243<br />
Document Type : Conference Paper<br />
Keywords : 802.11 MAC protocol, Adaptive bandwidth allocation, Bandwidth allocations, Distributed coordination functions, IEEE 802.11<br />
wireless LAN, Other applications, P2P applications, Remaining bandwidth, Streaming applications, Unfairness problem, Wireless<br />
access, Wireless client, Wireless devices, Wireless stations, Wireless traffic; Acoustic streaming, Information<br />
technology, Internet protocols, Jitter, Standards, Videotex, Wireless local area networks (WLAN); Bandwidth<br />
<strong>Abstract</strong> : According to the nature <strong>of</strong> IEEE 802.11 wireless LAN, the bandwidth is shared among wireless devices. However, the<br />
bandwidth could not be fairly shared among stations, because some users may use greedy applications which prevent other<br />
applications from connecting to the Internet. These applications are for example, video streaming applications, download<br />
accelerators and P2P applications. Moreover, Distributed Coordination Function (DCF) mechanism provided in the 802.11 MAC<br />
protocol originates the per-flow unfairness problem between uplink and downlink flows, as uplink flows usually dominate<br />
downlink flows and leads to unfair bandwidth allocation among wireless stations. In this paper, we propose an adaptive<br />
bandwidth allocation mechanism to provide per-station fairness. The proposed scheme can control the wireless traffic for<br />
fairness bandwidth allocation among stations. The bandwidth <strong>of</strong> each station can be adjusted to minimize the remaining<br />
bandwidth. The proposed scheme also covers a method to prevent domination <strong>of</strong> uplink flow which affects per-station<br />
fairness. Furthermore, the proposed scheme can be implemented on wireless access router. Hence, in order to support<br />
legacy wireless clients, our solution can be efficiently used. ©<strong>2010</strong> IEEE.<br />
No.1622<br />
Author (s) : Visudtibhan, A., Boonsopa, C., Thampratankul, L., Nuntnarumit, P., Okaschareon, C., Khongkhatithum, C., Chiemchanya, S.,<br />
Visudhiphan, P.<br />
Title : Headache in junior high school students: Types & characteristics in Thai children<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 5, May <strong>2010</strong>, Pages 550-557<br />
Document Type : Article<br />
590
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : Headache; Migraine; Prevalence; Schoolchildren; Thailand<br />
<strong>Abstract</strong> : Objective: To survey the prevalence, types, and characteristics <strong>of</strong> headache in junior high school students. Material and<br />
Method: A two-stage study was conducted in seventh grade students in Bangkok, Thailand. A screening self-administered<br />
check-list questionnaire and a face-to-face interview followed by physical examination were performed. Headache was<br />
diagnosed and classified according to the Second Edition <strong>of</strong> the Classification <strong>of</strong> Headache Disorder criteria. Results: 953<br />
students (448 boys and 505 girls, mean-age 13.2 years) in seventh grade, participated in the present study. Eight hundred<br />
thirty three students (87.4%) reported <strong>of</strong> having five episodes <strong>of</strong> headache in the past three months. After person-toperson<br />
interview; there were 121 (12.6%), 116 (12.1%), nine (0.9%), and seven (0.7%) students who had headache attributing to<br />
rhinosinusitis / upper respiratory tract infections, migraine, nonspecific headache with upper-limit systolic blood pressure, and<br />
tension-type headache respectively. Lack <strong>of</strong> sleep and stress related to daily school-activities were reported as headache<br />
precipitator in 25 and 23 students with migraine, respectively. School absenteeism was documented in four students with<br />
migraine. Only one student received migraine prophylactic treatment. Conclusion: Headache was common in Thai grade<br />
seven schoolchildren. Awareness <strong>of</strong> its high prevalence in these agegroup children will lead to early identification <strong>of</strong> students<br />
who may need intervention and specific treatment.<br />
No.1623<br />
Author (s) : Visudtibhan, A., Thampratankul, L., Khongkhatithum, C., Okascharoen, C., Siripornpanich, V., Chiemchanya, S., Visudhiphan, P.<br />
Title : Migraine in junior high-school students: A prospective 3-academic-year cohort study<br />
Source : Brain and Development, Volume 32, Issue 10, November <strong>2010</strong>, Pages 855-862<br />
Document Type : Article<br />
Keywords : Cohort study; Migraine; Schoolchildren; Thailand<br />
<strong>Abstract</strong> : Migraine is a common childhood illness with expected favorable outcome. A study <strong>of</strong> the long-term clinical course <strong>of</strong><br />
childhood migraine will provide information <strong>of</strong> evolution <strong>of</strong> migraine. A cohort study for 3-academic-year was conducted in<br />
Thai junior high-school children from July 2005 to February 2008 to determine the clinical course <strong>of</strong> migraine. Two hundred<br />
and forty-eight students in four junior high schools diagnosed with migraine according to ICHD-II in July 2005 were recruited.<br />
Each student was serially evaluated twice yearly from 7th grade during each semester <strong>of</strong> the academic year until the second<br />
semester <strong>of</strong> 9th grade. Determination <strong>of</strong> the characteristics, severity, frequency, and treatment <strong>of</strong> headache were obtained by<br />
questionnaire and direct interview. At the final evaluation, clinical course <strong>of</strong> headache was categorized into seven patterns.<br />
Among enrolled students, 209 (84.3%) completed the study. Twenty-eight (13.5%) students had no recurrent headache while<br />
that <strong>of</strong> 153 (73.5%) improved. No improvement <strong>of</strong> migraine and worsened migraine were observed in four students (1.8%)<br />
and 24 students (11.2%), respectively. Spontaneous remission and avoidance <strong>of</strong> precipitating causes contributed to relief <strong>of</strong><br />
migraine in the majority <strong>of</strong> the students. Stress-related daily school activities and inadequate rest were reported as common<br />
precipitating factors among students with non-improving or worsening outcome. Chronic daily headache and tension-type<br />
headache was observed in 6 and 30 students, respectively. This study confirms that clinical course <strong>of</strong> migraine in<br />
schoolchildren is benign. Frequency and intensity <strong>of</strong> headache can be reduced with reassurance and appropriate guidance.<br />
Early recognition and appropriate prevention <strong>of</strong> migraine attack will decrease the risk <strong>of</strong> chronic migraine and disease burden.<br />
© 2009 Elsevier B.V.<br />
No.1624<br />
Author (s) : Visudtibhan, A., Tuntiyathorn, L., Vaewpanich, J., Sukjit, P., Khongkatithum, C., Thampratankul, L., Chiemchanya, S.,<br />
Visudhiphan, P.<br />
Title : Acute disseminated encephalomyelitis: A 10-year cohort study in Thai children<br />
Source : European Journal <strong>of</strong> Paediatric Neurology, Volume 14, Issue 6, November <strong>2010</strong>, Pages 513-518<br />
Document Type : Article<br />
Keywords : ADEM; Children; Multiple sclerosis; Outcomes; Thailand<br />
<strong>Abstract</strong> : Childhood acute disseminated encephalomyelitis (ADEM) is a demyelinating disease with variable clinical courses and<br />
outcomes. Its evolution to multiple sclerosis in Asian children is yet to be determined. Medical records, investigation results<br />
and magnetic resonance imaging <strong>of</strong> brain <strong>of</strong> Thai children aged less than 15 years with initial diagnosis <strong>of</strong> ADEM at a referral<br />
university hospital in Thailand from January 1997 to December 2006 were reviewed. Clinical course and the outcome were<br />
finalized by telephone interview, self-report questionnaire, and/or neurological examination by December 2008. Modified<br />
Rankin Score was applied for determination <strong>of</strong> disability. MRI findings were categorized along with the locations and number<br />
<strong>of</strong> areas <strong>of</strong> abnormalities shown by T2-weight and FLAIR. 16 patients consisting <strong>of</strong> 5 boys and 11 girls (age-range 1-14 years,<br />
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mean 6.9 ± 3.6 years, median 6 years) were identified. Nine patients had cranial nerve dysfunctions including one child with<br />
optic neuropathy. One patient died with confirmed pathological diagnosis <strong>of</strong> ADEM. Among the remaining 15, who were<br />
followed from 2 to 10 years (mean 5.8 years), 13 and 3 patients were classified into monophasic ADEM and multiple sclerosis,<br />
respectively. Ten <strong>of</strong> 13 with final diagnosis <strong>of</strong> ADEM had complete recovery. There was no association between number <strong>of</strong><br />
lesions or location in the initial MRI and the outcome and final diagnosis. ADEM in Thai children had similar clinical<br />
presentation and outcome to previous studies in Western countries. ADEM can occasionally evolve to multiple sclerosis in<br />
Thai children as being shown in previous reports from other Asian countries. © <strong>2010</strong> European Paediatric Neurology Society.<br />
Published by Elsevier Ltd. All rights reserved.<br />
No.1625<br />
Author (s) : Vitta, A., Yoshino, T.P., Kalambaheti, T., Komalamisra, C., Waikagul, J., Ruangsittichai, J., Dekumyoy, P.<br />
Title : Application <strong>of</strong> recombinant SMR-domain containing protein <strong>of</strong> angiostrongylus cantonensis in immunoblot diagnosis <strong>of</strong> human<br />
angiostrongyliasis<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 4, July <strong>2010</strong>, Pages 785-799<br />
Document Type : Article<br />
Keywords : Angiostrongylus cantonensis; Eosinophilic meningitis; Immunodiagnosis; Recombinant fusion protein<br />
<strong>Abstract</strong> : The aim <strong>of</strong> this study was to find novel proteins expressed from an Angiostrongylus cantonensis adult female worm cDNA<br />
library for serodiagnosis <strong>of</strong> angiostrongyliasis. An immuno-dominant clone, fAC22, was identified by immunoscreening with<br />
pooled positive sera from proven angiostrongyliasis patients. The clone contained an open reading frame <strong>of</strong> 2,136 bp<br />
encoding a 80.5 kDa protein with a predicted isoelectric point <strong>of</strong> 5.8. The deduced amino acid sequence (712 amino acids)<br />
contained the conserved domain <strong>of</strong> Small mutS related (Smr) superfamily protein, with similarity with the Smr domain<br />
protein <strong>of</strong> Brugia malayi. The fusion His-tagged 81 kDa recombinant protein expressed as inclusion body in Escherichia coli<br />
was solubilized and purified by Ni-affinity chromatography for use in immunoblot analysis. Its sensitivity, specificity, positive<br />
and negative predictive values in immunodiagnostic test was 93.5, 91.5, 79.0 and 97.5%, respectively. Although some crossreactivity<br />
<strong>of</strong> the antigen was observed among gnathostomiasis, bancr<strong>of</strong>tian filariasis, ascariasis, echinococcosis, paragonimiasis<br />
and opisthorchiasis, sera from 14 other infections were all negative. These data indicate its possible application in<br />
immunodiagnosis <strong>of</strong> clinically suspected angiostrongyliasis.<br />
No.1626<br />
Author (s) : Vivithanaporn, P., Heo, G., Gamble, J., Krentz, H.B., Hoke, A., Gill, M.J., Power, C.<br />
Title : Neurologic disease burden in treated HIV/AIDS predicts survival: A population-based study<br />
Source : Neurology, Volume 75, Issue 13, 28 September <strong>2010</strong>, Pages 1150-1158<br />
Document Type : Article<br />
Keywords : acquired immune deficiency syndrome, adult, article, Canada, cause <strong>of</strong> death, CD4+ T lymphocyte, central nervous system<br />
infection, clinical feature, controlled study, distal sensory polyneuropathy, epilepsy, female, headache, health program, HIV<br />
associated dementia, human, Human immunodeficiency virus infected patient, Human immunodeficiency virus<br />
infection, major clinical study, male, mononeuropathy, morbidity, mortality, motor dysfunction, myopathy, neurologic<br />
disease, opportunistic infection, patient care, prevalence, priority journal, seizure, survival time, virus load, health care<br />
planning, incidence, prediction and forecasting, proportional hazards model, retrospective study; Adult, Community Health<br />
Planning, Female, HIV Infections, Humans, Incidence, Male, Nervous System Diseases, Predictive Value <strong>of</strong><br />
Tests, Prevalence, Proportional Hazards Models, Retrospective Studies<br />
<strong>Abstract</strong> : Background: Combination antiretroviral therapy (cART) has improved the survival <strong>of</strong> patients with HIV/AIDS but its impact<br />
remains uncertain on the changing prevalence and incidence <strong>of</strong> neurologic disorders with ensuing effects on mortality.<br />
Methods: The prevalence and incidence <strong>of</strong> neurologic disorders were examined in patients receiving active care in a regional<br />
HIV care program from 1998 to 2008. The mortality hazard ratio (HR) was calculated by Cox proportional hazard models with<br />
adjustment for demographic and clinical variables. Results: Of 1,651 HIV-infected patients assessed, 404 (24.5%) were<br />
identified as having one or more neurologic disorders, while 41% <strong>of</strong> AIDS-affected persons exhibited neurologic disease.<br />
Symptomatic distal sensory polyneuropathy (DSP, 10.0%) and HIV-associated neurocognitive disorder (HAND, 6.2%)<br />
represented the most prevalent disorders among 53 recognized neurologic disorders. Patients with at least one neurologic<br />
disorder exhibited higher mortality rates (17.6% vs 8.0%, p < 0.0001), particularly AIDS-related deaths (9.7% vs 3.2%, p <<br />
0.0001), compared with those without neurologic disorders. The highest mortality HR was associated with opportunistic<br />
infections <strong>of</strong> CNS (HR 5.3, 95% confidence interval [CI] 2.5-11.2), followed by HAND (HR 3.1, 95% CI 1.8-5.3) and the presence<br />
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<strong>of</strong> any neurologic disorder (HR 2.0, 95% CI 1.2-3.2). The risk <strong>of</strong> AIDS-related death with a neurologic disorder was increased by<br />
13.3% per 100 cells/mm decrement in blood CD4+ T-cell levels or by 39% per 10-fold increment in plasma viral load.<br />
Conclusions: The burden and type <strong>of</strong> HIV-related neurologic disease have evolved over the past decade and despite the<br />
availability <strong>of</strong> cART, neurologic disorders occur frequently and predict an increased risk <strong>of</strong> death. Copyright © <strong>2010</strong> by AAN<br />
Enterprises, Inc.<br />
No.1627<br />
Author (s) : Vivithanaporn, P., Maingat, F., Lin, L.-T., Na, H., Richardson, C.D., Agrawal, B., Cohen, E.A., Jhamandas, J.H., Power, C.<br />
Title : Hepatitis C virus core protein induces neuroimmune activation and potentiates human immunodeficiency virus-1 neurotoxicity<br />
Source : PLoS ONE, Volume 5, Issue 9, <strong>2010</strong>, Article number e12856, Pages 1-14<br />
Document Type : Article<br />
Keywords : beta tubulin, core protein, gamma interferon inducible protein 10, Human immunodeficiency virus protein, interleukin<br />
1beta, interleukin 6, interleukin 8, nonstructural protein 3, tumor necrosis factor alpha, virus RNA; animal experiment, animal<br />
model, article, astrocyte, behavior disorder, brain nerve cell, cell survival, cell viability, controlled<br />
study, encephalitis, female, gene expression, gliosis, Hepatitis C virus, human, human cell, human cell culture, Human<br />
immunodeficiency virus 1, Human immunodeficiency virus 1 infection, human<br />
tissue, immunomodulation, immunoreactivity, microglia, mouse, neurologic<br />
disease, neuropathology, neurotoxicity, nonhuman, virus culture; Animalia, Hepatitis C virus, Human immunodeficiency virus<br />
1, Mus musculus<br />
<strong>Abstract</strong> : Background: Hepatitis C virus (HCV) genomes and proteins are present in human brain tissues although the impact <strong>of</strong> HIV/ HCV<br />
co-infection on neuropathogenesis remains unclear. Herein, we investigate HCV infectivity and effects on neuronal survival<br />
and neuroinflammation in conjunction with HIV infection. Methodology: Human microglia, astrocyte and neuron cultures were<br />
infected with cell culture-derived HCV or exposed to HCV core protein with or without HIV-1 infection or HIV-1 Viral Protein R<br />
(Vpr) exposure. Host immune gene expression and cell viability were measured. Patch-clamp studies <strong>of</strong> human neurons were<br />
performed in the presence or absence <strong>of</strong> HCV core protein. Neurobehavioral performance and neuropathology were<br />
examined in HIV-1 Vpr-transgenic mice in which stereotaxic intrastriatal implants <strong>of</strong> HCV core protein were performed.<br />
Principal Findings: HCV-encoded RNA as well as HCV core and non-structural 3 (NS3) proteins were detectable in human<br />
microglia and astrocytes infected with HCV. HCV core protein exposure induced expression <strong>of</strong> pro-inflammatory cytokines<br />
including interleukin-1b, interleukin-6 and tumor necrosis factor-α in microglia (p
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Document Type : Article<br />
Keywords : Diamond bur; Hybrid resin composite; Hydr<strong>of</strong>luoric acid; Sandblast; Weibull analysis<br />
<strong>Abstract</strong> : The objective <strong>of</strong> this study was to analyze the survival probabilities <strong>of</strong> various surface preparation techniques for bonding<br />
buccal tubes on resin composite surface. Resin composite specimens (hybrid), 33 per group were prepared. 8 experimental<br />
groups were (1) sandblast 90 μm; (2) sandblast 50 μm; (3) abrasion using diamond bur; (4) hydr<strong>of</strong>luoric acid etching for 1 min<br />
(HF 1 min); (5) HF 2 min; (6) HF 3 min; (7) 37% orthophosphoric etching for 10 min (H3PO4 10 min) and (8) control (unetched<br />
surface). Plastic conditioner was applied then buccal tubes were bonded. Shear bond strength was tested with the universal<br />
testing machine and the Weibull analysis was performed. The results at a stress level <strong>of</strong> 7.8 MPa and 10 MPa, revealed the<br />
probabilities <strong>of</strong> survival for sandblast 50 μm (100%, 99.9%), sandblast 90 μm (99.7%, 98.7%), diamond bur (98.3%, 93.4%), HF<br />
2 min (95.9%, 85.3%), HF 1 min (78.3%, 61.4%), HF 3 min (70.6%, 46.3%), H3PO4 10 min (61.4%, 44.5%) and control (26.3%,<br />
6.9%), respectively. Therefore, surface preparation by sandblast (50 μm and 90 μm), or diamond bur followed by plastic<br />
conditioner were highly effective for bonding orthodontic buccal tubes on hybrid resin composite surface. HF 2 min was<br />
acceptable. However, HF 1 min, 3 min, H3PO4 etching and unetched surface could not achieve adequate bond survival. ©<br />
<strong>2010</strong> Elsevier Ltd and the Japanese Orthodontic Society.<br />
No.1630<br />
Author (s) : Viwattanatipa, N., Jermwiwatkul, W., Chintavalakorn, R., Nanthavanich, N.<br />
Title : The effect <strong>of</strong> different surface preparation techniques on the survival probabilities <strong>of</strong> orthodontic brackets bonded to nan<strong>of</strong>ill<br />
composite resin<br />
Source : Journal <strong>of</strong> Orthodontics, Volume 37, Issue 3, September <strong>2010</strong>, Pages 162-173<br />
Document Type : Article<br />
Keywords : Diamond bur; Hydr<strong>of</strong>luoric acid; Nan<strong>of</strong>ill resin composite; Sandblast; Weibull analysis<br />
<strong>Abstract</strong> : Objective: To analyze the survival probabilities <strong>of</strong> different surface preparation techniques for bonding brackets to nan<strong>of</strong>ill<br />
composite resin. Design: In vitro, laboratory study. Setting: <strong>Mahidol</strong> <strong>University</strong>, Bangkok, Thailand. Materials and methods:<br />
Thirty-five nan<strong>of</strong>ill composite resin specimens/group were subjected to four surface preparation techniques as follows: (1)<br />
sandblast using aluminium oxide powder <strong>of</strong> 90 μm; (2) abrasion using diamond bur; (3) hydr<strong>of</strong>luoric acid etching for 2 min;<br />
and (4) 37% orthophosphoric acid etching for 30 s. Plastic conditioner was applied then brackets were bonded. Shear bond<br />
strength tests were carried out on a universal testing machine. Main outcome measures: Shear bond strength (MPa) and<br />
debonding force (N) were analyzed using Weibull analysis Results: The maximum stress and debonding force levels with a<br />
95% probability <strong>of</strong> survival ranking from highest to lowest were: (1) sandblast group (4.2 MPa, 45.5 N); (2) diamond bur group<br />
(2.2 MPa, 25.3 N); (3) orthophosphoric group (1.9 MPa, 19.8 N); and (4) hydr<strong>of</strong>luoric group (0.8 MPa, 10.9 N). There was a<br />
significant difference in the adhesive remnant index scores between the surface preparation techniques (chi squared P<<br />
0.001). Conclusion: Bonding orthodontic brackets to nan<strong>of</strong>ill composite resin materials may result in lower bond strengths and<br />
special surface preparation techniques might be required to avoid increased numbers <strong>of</strong> bond failures. Surface treatment with<br />
sandblasting followed by plastic conditioner could increase the survival probability. The use <strong>of</strong> a diamond bur,<br />
orthophosphoric etching or hydr<strong>of</strong>luoric etching cannot be recommended. © <strong>2010</strong> British Orthodontic Society.<br />
No.1631<br />
Author (s) : Von Hertzen, H., Huong, N.T.M., Piaggio, G., Bayalag, M., Cabezas, E., Fang, A.H., Gemzell-Danielsson, K., Hinh, N.D., Mittal, S., Ng,<br />
E.H.Y., Chaturachinda, K., Pinter, B., Puscasiu, L., Savardekar, L., Shenoy, S., Khomassuridge, A., Tuyet, H.T.D., Velasco, A.,<br />
Peregoudov, A.<br />
Title : Misoprostol dose and route after mifepristone for early medical abortion: A randomised controlled noninferiority trial<br />
Source : BJOG: An <strong>International</strong> Journal <strong>of</strong> Obstetrics and Gynaecology, Volume 117, Issue 10, September <strong>2010</strong>, Pages 1186-1196<br />
Document Type : Article<br />
Keywords : Factorial design; medical abortion; mifepristone; misoprostol dose and route<br />
<strong>Abstract</strong> : Objective To compare 400 and 800 g sublingual or vaginal misoprostol 24 hours after 200 mg mifepristone for noninferiority<br />
regarding efficacy in achieving complete abortion for pregnancy termination up to 63 days <strong>of</strong> gestation. Design Placebocontrolled,<br />
randomised, noninferiority factorial trial, stratified by centre and length <strong>of</strong> gestation. Misoprostol 400 or 800 g,<br />
administered either sublingually or vaginally, with follow up after 2 and 6 weeks. Setting Fifteen obstetricsgynaecology<br />
departments in ten countries. Population Pregnant women (n = 3005) up to 63 days <strong>of</strong> gestation requesting medical abortion.<br />
Methods Two-sided 95% CI for differences in failure <strong>of</strong> complete abortion and continuing pregnancy, with a 3% noninferiority<br />
margin, were calculated. Proportions <strong>of</strong> women with adverse effects were recorded. Outcome measures Complete abortion<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
without surgical intervention (main); continuing live pregnancies, induction-to-abortion interval, adverse effects, women's<br />
perceptions (secondary). Results Efficacy outcomes analysed for 2962 women (98.6%): 90.5% had complete abortion after<br />
400 g misoprostol, 94.2% after 800 g. Noninferiority <strong>of</strong> 400 g misoprostol was not demonstrated for failure <strong>of</strong> complete<br />
abortion (difference: 3.7%; 95% CI 1.8-5.6%). The 400-g dose showed higher risk <strong>of</strong> incomplete abortion (P < 0.01) and<br />
continuing pregnancy (P < 0.01) than 800 g. Vaginal and sublingual routes had similar risks <strong>of</strong> failure to achieve complete<br />
abortion (P = 0.47, difference in sublingual minus vaginal -0.7%, 95% CI -2.6-1.2%). A similar pattern was observed for<br />
continuing pregnancies (P = 0.21). Fewer women reported adverse effects with vaginal than sublingual administration and with<br />
the 400-g dose than the 800-g dose. Of the women, 94% were satisfied or highly satisfied with the regimens, 53% preferred<br />
the sublingual route and 47% preferred the vaginal route. Conclusions A 400-g dose <strong>of</strong> misoprostol should not replace the<br />
800-g dose when administered 24 hours after 200 mg mifepristone for inducing abortion in pregnancies up to 63 days.<br />
Sublingual and vaginal misoprostol have similar efficacy, but vaginal administration is associated with a lower frequency <strong>of</strong><br />
adverse effects. © RCOG <strong>2010</strong> BJOG An <strong>International</strong> Journal <strong>of</strong> Obstetrics and Gynaecology.<br />
No.1632<br />
Author (s) : Vonghachack, Y., Dekumyoy, P., Yoonuan, T., Sa-nguankiat, S., Nuamtanong, S., Thaenkham, U., Phommasack, B., Kobayashi, J.,<br />
Waikagul, J.<br />
Title : Sero-epidemiological survey <strong>of</strong> gnathostomiasis in Lao PDR<br />
Source : Parasitology <strong>International</strong>, Volume 59, Issue 4, December <strong>2010</strong>, Pages 599-605<br />
Document Type : Article<br />
Keywords : Food-borne nematode; Gnathostoma spinigerum; Gnathostomiasis; Immunoblot; Lao PDR; Sero-prevalence<br />
<strong>Abstract</strong> : While human gnathostomiasis cases have been reported sporadically in Lao PDR since 1975, little is known about the disease<br />
in this country. We aimed to investigate sero-prevalence <strong>of</strong> gnathostomiasis and Gnathostoma species in Lao PDR. One village<br />
each in the north, central and south regions <strong>of</strong> Lao PDR was selected as the study sites. Overall, 125 (29.8%) <strong>of</strong> 420 sera from<br />
the randomly selected participants were sero-positive by immunoblot technique, with anti-Gnathostoma IgG antibody against<br />
the 24. kDa fraction. The sero-prevalence was high in the central (47.1%) and south (38.6%), but low (3.6%) in the north. Risk<br />
factor analyses revealed that the consumption <strong>of</strong> raw/undercooked fish was significantly associated with Gnathostoma seropositivity<br />
(95% CI 1.05-17.05, P=0.042). The sero-positivity significantly increased with the age <strong>of</strong> the participants. Several fish,<br />
swamp eels, and frogs collected from central and southern Lao were infected with G. spinigerum advanced 3rd-stage larvae.<br />
Channa limbata (red-tailed snakehead fish) was identified as a natural second intermediate host <strong>of</strong> G. spinigerum. Eggs <strong>of</strong> G.<br />
spinigerum were found in dog feces collected in the south. Gnathostomiasis is endemic in central and southern Laos, so that<br />
preventive measures should be introduced for people living in these regions. © <strong>2010</strong> Elsevier Ireland Ltd.<br />
No.1633<br />
Author (s) : Vongsawan, K., Surarit, R., Rirattanapong, P.<br />
Title : The effect <strong>of</strong> high calcium milk and casein phosphopeptide-amorphous calcium phosphate on enamel erosion caused by<br />
cholinated water<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 6, November <strong>2010</strong>, Pages 1494-1499<br />
Document Type : Article<br />
Keywords : Casein phosphopeptide-amorphous calcium phosphate; Erosion; Microhardness; Milk<br />
<strong>Abstract</strong> : The aim <strong>of</strong> this study was to determine the effectiveness <strong>of</strong> high calcium milk and casein phosphopeptide-amorphous<br />
calcium phosphate (CPP-ACP) on enamel erosion caused by chlorinated water. Thirty-six bovine enamel samples without<br />
wear or caries 3×4 mm in size were placed in acrylic blocks. All specimens were randomly allocated into 3 groups<br />
(n=12/group): CPP-ACP in the form <strong>of</strong> paste, Anlene concentrated milk and a control (no treatment). All specimens were<br />
soaked in chlorinated water (pH =5.0) at room temperature for 72 hours following by soaking in artificial saliva for 30 minutes.<br />
Then, microhardness was determined using a microhardness tester. Data were analyzed using a one-way ANOVA and paired ttest.<br />
The microhardness value change in the control group was significantly higher than the other groups. No significant<br />
differences were seen between the 2 study groups. High calcium milk and CPP-ACP enhanced remineralization <strong>of</strong> enamel<br />
erosion caused by chlorinated water.<br />
No.1634<br />
Author (s) : Vongvatcharanon, U., Khornchatri, K., Udomuksorn, W., Kumarnsit, E., Vongvatcharanon, S., Sobhon, P.<br />
Title : Influence <strong>of</strong> aging and long-term swimming exercise on parvalbumin distribution in rat hearts<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Source : Acta Histochemica, Volume 112, Issue 1, January <strong>2010</strong>, Pages 72-80<br />
Document Type : Article<br />
Keywords : Aging; Calcium-binding protein; Heart; Immunohistochemistry; Parvalbumin; Swimming; Western blotting<br />
<strong>Abstract</strong> : Parvalbumin (PV), which is a small (12 kDa) cytoplasmic calcium-binding protein, has been implicated in mediating relaxation<br />
in cardiac myocytes. The influence <strong>of</strong> aging and exercise on the distribution <strong>of</strong> PV in rat heart was investigated. Male Wistar<br />
rats aged 3, 6, 12 and 18-months were divided into sedentary and exercise groups. The exercise group underwent exercise in<br />
the form <strong>of</strong> regular swimming for 6 months. The hearts were processed for immunohistochemistry and Western blotting. The<br />
intensity <strong>of</strong> PV immunoreactivity was strong in the 9 and 12-month hearts and decreased in the 18-month hearts. The<br />
smallest amount was detected in the 24-month rat hearts when compared to those <strong>of</strong> the 9, 12 and 18-month rat hearts.<br />
Significantly less PV was detected in the 18 and 24-month hearts compared to the 12-month rat hearts (P
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
close to the anterior surface <strong>of</strong> the sacroiliac joint and the ala <strong>of</strong> the sacrum. To prevent nerve root injury, dissection with a<br />
sharp instrument should be avoided at such area and 5 to 7 mm medial to the sacroiliac joint.<br />
No.1637<br />
Author (s) : Wajanavisit, W., Woratanarat, P., Woratanarat, T., Aroonjaruthum, K., Kulachote, N., Leelapatana, W., Laohacharoensombat, W.<br />
Title : The evaluation <strong>of</strong> short fusion in idiopathic scoliosis<br />
Source : Indian Journal <strong>of</strong> Orthopaedics, Volume 44, Issue 1, 1 January <strong>2010</strong>, Pages 28-34<br />
Document Type : Article<br />
Keywords : Idiopathic scoliosis; Pedicle screw and plate fixation; Selective posterior fusion; Short fusion<br />
<strong>Abstract</strong> : Background: Selective thoracic fusion in type II curve has been recommended by King et al. since 1983. They suggested that<br />
care must be taken to use the vertebra that is neutral and stable so that the lower level <strong>of</strong> fusion is centered over the<br />
sacrum. Since then there has been the trend to do shorter and selective fusion <strong>of</strong> the major curve. This study was conducted<br />
to find out whether short posterior pedicle instrumentation alone could provide efficient correction and maintain trunk<br />
balance comparing to the anterior instrumentation. Materials and Methods: A prospective study was conducted during 2005-<br />
2007 on 39 consecutive cases with idiopathic scoliosis cases King 2 and 3 (Lenke 1A, 1B), 5C and miscellaneous. Only the<br />
major curve was instrumented unless both curves were equally rigid and <strong>of</strong> the same magnitude. The level <strong>of</strong> fusion was<br />
planned as the end vertebra (EVB) to EVB fusion, although minor adjustment was modified by the surgeons intraoperatively.<br />
The most common fusion levels in major thoracic curves were T6-T12, whereas the most common fusion levels in the<br />
thoraco-lumbar curves were T10-L3. Fusion was performed from the posterior only approach and the implants utilized were<br />
uniformly plate and pedicle screw system. All the patients were followed at least 2 years till skeletal maturity. The correction<br />
<strong>of</strong> the curve were assessed according to type <strong>of</strong> curve (lenke IA, IB and 5), severity <strong>of</strong> curve (less than 450, 450-890 and more<br />
than 900), age at surgery (14 or less and 15 or more) and number <strong>of</strong> the segment involved in instrumentation (fusion level<br />
less than curve, fusion level as <strong>of</strong> the curve and fusion more than the curve) Results: The average long-term curve correction<br />
for the thoracic was 40.4% in Lenke 1A, 52.2% in Lenke 1B and 56.3% in Lenke 5. The factors associated with poorer outcome<br />
were younger age at surgery ( 11 years or Risser 0), fusion at wrong levels (shorter than the measured end vertebra) and rigid<br />
curve identified by bending study. However, all patients had significant improved trunk balance and coronal hump at the final<br />
assessment at maturity. Two patients underwent late extension fusion because <strong>of</strong> junctional scoliosis. Conclusions: With<br />
modern instrumentations, the EVB <strong>of</strong> the major curve can be used at the end <strong>of</strong> the instrumentation in most cases <strong>of</strong><br />
idiopathic scoliosis. In those cases with either severe trunk shift, younger than 11 years old, or extreme rigid curve, an<br />
extension <strong>of</strong> one or more levels might be safer. In particular situations, the concept <strong>of</strong> centering the lowest vertebra over the<br />
sacrum should be adopted.<br />
No.1638<br />
Author (s) : Waltenberger, B.; Schuster, D.; Paramapojn, S.; Gritsanapan, W.; Wolber, G.; Rollinger, J.; Stuppner, H.<br />
Title : In silico strategy for the identification <strong>of</strong> cyclooxygenase inhibitors from the Thai medicinal mixture Prasaplai<br />
Source : PLANTA MEDICA, Volume: 76, Issue: 12, Pages: 1173-1173, Published: AUG <strong>2010</strong><br />
Document Type : Meeting Abdtract<br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1639<br />
Author (s) : Wananukul, W., Klaikleun, S., Sriapha, C., Tongpoo, A.<br />
Title : Effect <strong>of</strong> activated charcoal in reducing paracetamol absorption at a supra-therapeutic dose<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 10, October <strong>2010</strong>, Pages 1145-1149<br />
Document Type : Article<br />
Keywords : Acetaminophen; Activated charcoal; Cross-over; Decontamination; Overdose; Paracetamol; Pharmacokinetics; Randomization<br />
<strong>Abstract</strong> : Background: Activated charcoal (AC) is recommended for treatment <strong>of</strong> acute poisoning, thereby decreasing gastrointestinal<br />
tract absorption. AC from different sources may have different adsorptive capacity. The AC that is available in Thailand has<br />
not been proven yet for its efficacy. The authors simulated paracetamol overdose model for the present study. Objective: To<br />
assess the efficacy <strong>of</strong> AC that is available in Thailand in decreasing absorption <strong>of</strong> paracetamol at supratherapeutic dose.<br />
Material and Method: This was a two-arm, prospective, crossover study. Washout period was 1 week. Twelve healthy male<br />
volunteers participated. All volunteers were randomly assigned to either sequence <strong>of</strong> control-experiment (CE) or EC. The<br />
597
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
participants ingested 60 mg/Kg <strong>of</strong> paracetamol at Time = 0. At Time = 0.25 hour, they ingested 50 g <strong>of</strong> AC as slurry with 250<br />
ml <strong>of</strong> water when they were assigned as E, but drank 250 mL <strong>of</strong> water when were assigned as C. Blood samples were serially<br />
collected for determination <strong>of</strong> paracetamol concentration and calculating pharmacokinetic parameters, area under the<br />
timeconcentration curve (AUC (0,∞)). Results: Means <strong>of</strong> the AUC (0,∞) were 313.7 ± 29.8 and 184.8 ± 91.6 mg-h/L in the<br />
control and experimental arm, respectively. It was statistically different (p = 0.01). Conclusion: The tested AC was found to be<br />
able to reduce the absorption <strong>of</strong> the supratherapeutic dose <strong>of</strong> paracetamol.<br />
No.1640<br />
Author (s) : Wangdi, K., Singhasivanon, P., Silawan, T., Lawpoolsri, S., White, NJ., Kaewkungwal, J.<br />
Title : Development <strong>of</strong> temporal modelling for forecasting and prediction <strong>of</strong> malaria infections using time-series and ARIMAX<br />
analyses: A case study in endemic districts <strong>of</strong> Bhutan<br />
Source : MALARIA JOURNAL Volume: 9 Article Number: 251 DOI: 10.1186/1475-2875-9-251 Published: SEP 3 <strong>2010</strong><br />
Document Type : Article<br />
Keywords : PLASMODIUM-FALCIPARUM; CLIMATIC VARIABLES; SRI-LANKA; TRANSMISSION; THAILAND; BURDEN; RISK<br />
<strong>Abstract</strong> : Background: Malaria still remains a public health problem in some districts <strong>of</strong> Bhutan despite marked reduction <strong>of</strong> cases in<br />
last few years. To strengthen the country's prevention and control measures, this study was carried out to develop<br />
forecasting and prediction models <strong>of</strong> malaria incidence in the endemic districts <strong>of</strong> Bhutan using time series and ARIMAX.<br />
Methods: This study was carried out retrospectively using the monthly reported malaria cases from the health centres to<br />
Vector-borne Disease Control Programme (VDCP) and the meteorological data from Meteorological Unit, Department <strong>of</strong><br />
Energy, Ministry <strong>of</strong> Economic Affairs. Time series analysis was performed on monthly malaria cases, from 1994 to 2008, in<br />
seven malaria endemic districts. The time series models derived from a multiplicative seasonal autoregressive integrated<br />
moving average (ARIMA) was deployed to identify the best model using data from 1994 to 2006. The best-fit model was<br />
selected for each individual district and for the overall endemic area was developed and the monthly cases from January to<br />
December 2009 and <strong>2010</strong> were forecasted. In developing the prediction model, the monthly reported malaria cases and the<br />
meteorological factors from 1996 to 2008 <strong>of</strong> the seven districts were analysed. The method <strong>of</strong> ARIMAX modelling was<br />
employed to determine predictors <strong>of</strong> malaria <strong>of</strong> the subsequent month. Results: It was found that the ARIMA (p, d, q) (P, D,<br />
Q)(s) model (p and P representing the auto regressive and seasonal autoregressive; d and D representing the non-seasonal<br />
differences and seasonal differencing; and q and Q the moving average parameters and seasonal moving average parameters,<br />
respectively and s representing the length <strong>of</strong> the seasonal period) for the overall endemic districts was (2,1,1)(0,1,1) 12; the<br />
modelling data from each district revealed two most common ARIMA models including (2,1,1)(0,1,1)(12) and (1,1,1)(0,1,1)(12).<br />
The forecasted monthly malaria cases from January to December 2009 and <strong>2010</strong> varied from 15 to 82 cases in 2009 and 67<br />
to 149 cases in <strong>2010</strong>, where population in 2009 was 285,375 and the expected population <strong>of</strong> <strong>2010</strong> to be 289,085. The<br />
ARIMAX model <strong>of</strong> monthly cases and climatic factors showed considerable variations among the different districts. In general,<br />
the mean maximum temperature lagged at one month was a strong positive predictor <strong>of</strong> an increased malaria cases for four<br />
districts. The monthly number <strong>of</strong> cases <strong>of</strong> the previous month was also a significant predictor in one district, whereas no<br />
variable could predict malaria cases for two districts. Conclusions: The ARIMA models <strong>of</strong> time-series analysis were useful in<br />
forecasting the number <strong>of</strong> cases in the endemic areas <strong>of</strong> Bhutan. There was no consistency in the predictors <strong>of</strong> malaria cases<br />
when using ARIMAX model with selected lag times and climatic predictors. The ARIMA forecasting models could be employed<br />
for planning and managing malaria prevention and control programme in Bhutan.<br />
No.1641<br />
Author (s) : Wangsomboonsiri, W., Mahasirimongkol, S., Chantarangsu, S., Kiertiburanakul, S., Charoenyingwattana, A., Komindr, S.,<br />
Thongnak, C., Mushiroda, T., Nakamura, Y., Chantratita, W., Sungkanuparph, S.<br />
Title : Association between HLA-B*4001 and lipodystrophy among HIV-infected patients from Thailand who received a stavudinecontaining<br />
antiretroviral regimen<br />
Source : Clinical Infectious Diseases, Volume 50, Issue 4, 15 February <strong>2010</strong>, Pages 597-604<br />
Document Type : Article<br />
Keywords : HLA DPB1 antigen, HLA DQB1 antigen, proteinase inhibitor, RNA directed DNA polymerase inhibitor, stavudine; adult, antiviral<br />
therapy, article, body composition, controlled study, disease severity, female, genetic risk, HIV associated<br />
lipodystrophy, human, Human immunodeficiency virus infection, major clinical study, male, patient coding, priority<br />
journal, risk assessment, Thailand; Adult, Anti-Retroviral Agents, Body Composition, Case-Control Studies, Cross-Sectional<br />
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Studies, Female, HIV Infections, HLA-B Antigens, Humans, Lipodystrophy, Logistic Models, Male, Middle Aged, Predictive Value<br />
<strong>of</strong> Tests, Risk Factors, Sensitivity and Specificity, Stavudine, Thailand<br />
<strong>Abstract</strong> : Background. Stavudine-containing antiretroviral regimens are widely used in developing countries. Stavudineassociated<br />
lipodystrophy commonly occurs, without a clear predictable pattern owing to the unknown interaction between stavudine<br />
and the host, among patients who received this regimen. The aim <strong>of</strong> this study was to determine the clinical risk factors and<br />
human leukocyte antigen (HLA) alleles associated with stavudine-associated lipodystrophy. Methods. A case-control, crosssectional<br />
study was conducted for HIV-infected patients receiving stavudinecontaining antiretroviral regimens. Clinical<br />
assessments for lipodystrophy by physical examination, anthropometry, and dual-energy X-ray absorptiometry were obtained.<br />
On the basis <strong>of</strong> their clinical assessment, the patients were classified into 2 groups: the case group (moderated to severe<br />
lipodystrophy) and the control group (absent to mild lipodystrophy). The clinical characteristics and allelic distribution <strong>of</strong><br />
HLA-A, HLA-B, HLA-C, HLA-DRB1, HLA-DQB1, and HLA-DPB1 were compared between the case group and the control group, to<br />
determine the possible association with stavudine-associated lipodystrophy. Results. There were 103 patients; 55 patients<br />
were in the case group, and 48 patients were in the control group. By use <strong>of</strong> forward stepwise logistic regression, the<br />
presence <strong>of</strong> HLA-B*4001 (odds ratio [OR], 14.05; 95% confidence interval [CI], 2.57-76.59; P =.002) and a longer duration <strong>of</strong><br />
stavudine treatment (OR, 1.02; 95% CI, 1.00-1.04; P =.02) were significantly associated with stavudine-associated lipodystrophy,<br />
whereas a higher body mass index during treatment (OR, 0.73; 95% CI, 0.61-0.86; P
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
from medical records for the same period. Unit cost calculation was based on the <strong>of</strong>ficial method <strong>of</strong> hospital accounting.<br />
Results The overall unit cost for each patient visit was $673.99 United States dollar (USD). The detailed unit cost <strong>of</strong> nurse<br />
visit, new patient visit, follow-up visit, consultation, Mohs and non-Mohs procedure were, respectively, $368.27, $580.09,<br />
$477.82, $585.52, $1,086.12 and $858.23 USD. With respect to a Mohs visit, the unit cost per lesion and unit cost per stage<br />
were $867.89 and $242.30 USD respectively. Conclusions Results from this retrospective study provide information that may<br />
be used for pricing strategy and resource allocation by the administrative board <strong>of</strong> MDU. © 2009 European Academy <strong>of</strong><br />
Dermatology and Venereology.<br />
No.1644<br />
Author (s) : Wasant, P., Kuptanon, C., Vattanavicharn, N., Liammongkolkul, S., Ratanarak, P., Sangruchi, T., Yamaguchi, S.<br />
Title : Glutaric aciduria type 2, late onset type in thai siblings with myopathy<br />
Source : Pediatric Neurology, Volume 43, Issue 4, October <strong>2010</strong>, Pages 279-282<br />
Document Type : Article<br />
Keywords : acylcarnitine, carnitine, creatine kinase, electron transferring flavoprotein, glycine, lactate dehydrogenase,<br />
methylprednisolone, prednisolone, rib<strong>of</strong>lavin; allele, article, case report, child, corticosteroid therapy, creatine kinase blood<br />
level, echocardiography, electromyography, follow up, gene mutation, human, human tissue, immunoblotting, lactate<br />
dehydrogenase blood level, lipid storage, low fat diet, male, multiple acyl CoA dehydrogenase deficiency, muscle<br />
biopsy, muscle strength, muscle weakness, myopathy, nerve conduction, physical examination, polymyositis, priority<br />
journal, protein deficiency, school child, sibling, Thailand; Alleles, Blotting, Western, Child, DNA Mutational Analysis, Electron-<br />
Transferring Flavoproteins, Humans, Iron-Sulfur Proteins, Male, Multiple Acyl Coenzyme A Dehydrogenase Deficiency, Muscle<br />
Weakness, Oxidoreductases Acting on CH-NH Group Donors<br />
<strong>Abstract</strong> : Reported here is a novel presentation <strong>of</strong> late onset glutaric aciduria type 2 in two Thai siblings. A 9-year-old boy presented<br />
with gradual onset <strong>of</strong> proximal muscle weakness for 6 weeks. The initial diagnosis was postviral myositis, and then<br />
polymyositis. Electromyography and nerve conduction velocity testing indicated a myopathic pattern. Muscle biopsy revealed<br />
excessive accumulation <strong>of</strong> fat. Acylcarnitine pr<strong>of</strong>iling led to the diagnosis <strong>of</strong> glutaric aciduria type 2. Immunoblot analysis <strong>of</strong><br />
electron-transferring-flavoprotein and its dehydrogenase electron-transferring-flavoprotein dehydrogenase led to mutation<br />
analysis <strong>of</strong> the ETFDH gene, which revealed two different pathogenic mutations in both alleles and confirmed the diagnosis <strong>of</strong><br />
glutaric aciduria type 2 caused by electron-transferring-flavoprotein dehydrogenase deficiency. The boy recovered completely<br />
after treatment. Later, his younger sibling became symptomatic; the same diagnosis was confirmed, and treatment was<br />
similarly effective. Acylcarnitine pr<strong>of</strong>iling was a crucial investigation in making this diagnosis in the presence <strong>of</strong> normal urine<br />
organic acid findings. Late onset glutaric aciduria type 2, a rare cause <strong>of</strong> muscle weakness in children, should be included in<br />
the differential diagnosis <strong>of</strong> myopathy. © <strong>2010</strong> by Elsevier Inc. All rights reserved.<br />
No.1645<br />
Author (s) : Wasant, P.; Sathienkijkanchai, A.; Vattanavicham, N.; Liammongkolkul, S.; Keeratichamroen, S.; Ketudat-Cairns, J. R.; Svasti, J.;<br />
Kolodny, E. H.<br />
Title : MUCOPOLYSACCHARIDOSES IN THAILAND: SIRIRAJ EXPERIENCE<br />
Source : JOURNAL OF INHERITED METABOLIC DISEASE, Volume: 33, Pages: S138-S138, Supplement: 1, Published: AUG <strong>2010</strong><br />
Document Type : Meeting Abdtract<br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1646<br />
Author (s) : Wasay, M., Kaul, S., Menon, B., Venketasubramanian, N., Gunaratne, P., Khalifa, A., Poungvarin, N., Saadatnia, M., Gan, R.N., Dai,<br />
A., Mehndiratta, M.M.<br />
Title : Ischemic stroke in young Asian women: Risk factors, subtypes and outcome<br />
Source : Cerebrovascular Diseases, Volume 30, Issue 4, September <strong>2010</strong>, Pages 418-422<br />
Document Type : Article<br />
Keywords : Asian; Female; Outcome; Stroke; Women; Young<br />
<strong>Abstract</strong> : Background and Objective: The objective <strong>of</strong> our study was to describe risk factors, mechanisms and outcome <strong>of</strong> young Asian<br />
women with ischemic stroke. Methods: Twelve tertiary-care centers in 8 Asian countries participated. Women aged 15-45<br />
years were included if they had an ischemic stroke supported by neuroimaging. Data on age, risk factor history, stroke<br />
600
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
mechanism and discharge status were collected. Results: A total <strong>of</strong> 958 subjects were included, their mean age was 34 years.<br />
Large-vessel thrombosis comprised 24%, cerebral venous thrombosis 21%, cardioembolism 19% and small-vessel thrombosis<br />
15%. The stroke risk factors included hypertension (29%), diabetes (14%), pregnancy (11%), valvular heart disease (10%) and<br />
cigarette smoking (3%). Anemia was found in 42%, and mortality was 4%; at discharge, 17% had modified Rankin score (mRS)<br />
>4 and 83% mRS 0-3. Conclusion: Unlike among Caucasians, large-vessel thrombosis, cerebral venous thrombosis and<br />
cardioembolism are common among young Asian women with stroke. A high proportion are pregnancy-related. More studies<br />
are needed. Copyright © <strong>2010</strong> S. Karger AG.<br />
No.1647<br />
Author (s) : Wasinphongwanit, P., Phokharatkul, P.<br />
Title : Image retrieval using contour feature with rough set method<br />
Source : <strong>2010</strong> <strong>International</strong> Conference on Computer, Mechatronics, Control and Electronic Engineering, CMCE <strong>2010</strong>, Volume 6, <strong>2010</strong>,<br />
Article number 5609831, Pages 349-352<br />
Document Type : Conference Paper<br />
Keywords : Fourier descriptors; Image retrieval; Rough set<br />
<strong>Abstract</strong> : Content based image retrieval (CBIR) is well-known in the field <strong>of</strong> image retrieval. It uses contents <strong>of</strong> an image from image<br />
processing and analysis to retrieve images that users were looking for from an image search. Shape based image retrieval was<br />
focused in this paper. A data mining was considered to find knowledge in the image database. Fourier descriptor is a most<br />
technique to extract contour feature <strong>of</strong> images. It was used to analyze the testing and training images in the preprocessing<br />
step. Fourier coefficients were quantized into multiple attributes and rough set theory was used to generate a rule-based<br />
system. Rough set theory is used as a data mining technique. It was compared to similarity measurement. We use 15, 984<br />
testing image data with 71, 928 training image data in this experiment. A total usage time <strong>of</strong> rough set method is 13, 286<br />
seconds. A total usage time <strong>of</strong> similarity measurement is 19, 365 seconds. A total usage memory <strong>of</strong> rough set method and<br />
similarity measurement are 2.8 Mbytes and 8.6 Mbytes respectively. An average precision, an average recall and an average<br />
accuracy <strong>of</strong> rough set method are 0.1297, 0.261 and 0.9971. An average precision, an average recall and an average accuracy<br />
<strong>of</strong> similarity measurement are 0.1619, 0.9651 and 0.9852. The rough set method is advantage to the usage time and the usage<br />
memory. © <strong>2010</strong> IEEE.<br />
No.1648<br />
Author (s) : Wasinpiyamongkol, L., Patramool, S., Luplertlop, N., Surasombatpattana, P., Doucoure, S., Mouchet, F., Séveno, M., Remoue,<br />
F., Demettre, E., Brizard, J.-P., Jouin, P., Biron, D.G., Thomas, F., Missé, D.<br />
Title : Blood-feeding and immunogenic Aedes aegypti saliva proteins<br />
Source : Proteomics, Volume 10, Issue 10, May <strong>2010</strong>, Pages 1906-1916<br />
Document Type : Article<br />
Keywords : Aedes; Animal proteomics; Blood meal; Dengue; Midgut; Salivary glands<br />
<strong>Abstract</strong> : Mosquito-transmitted pathogens pass through the insect's midgut (MG) and salivary gland (SG). What occurs in these organs in<br />
response to a blood meal is poorly understood, but identifying the physiological differences between sugar-fed and bloodfed<br />
(BF) mosquitoes could shed light on factors important in pathogens transmission. We compared differential protein<br />
expression in the MGs and SGs <strong>of</strong> female Aedes aegypti mosquitoes after a sugar- or blood-based diet. No difference was<br />
observed in the MG protein expression levels but certain SG proteins were highly expressed only in BF mosquitoes. In sugarfed<br />
mosquitoes, housekeeping proteins were highly expressed (especially those related to energy metabolism) and actin was<br />
up-regulated. The immun<strong>of</strong>luorescence assay shows that there is no disruption <strong>of</strong> the SG cytoskeletal after the blood meal.<br />
We have generated for the first time the 2-DE pr<strong>of</strong>iles <strong>of</strong> immunogenic Ae. aegypti SG BF-related proteins. These new data<br />
could contribute to the understanding <strong>of</strong> the physiological processes that appear during the blood meal. © <strong>2010</strong> Wiley-VCH<br />
Verlag GmbH & Co. KGaA.<br />
No.1649<br />
Author (s) : Watcharananan, S., Kiertiburanakul, S., Chantratita, W.<br />
Title : Rapid influenza diagnostic test during the outbreak <strong>of</strong> the novel influenza A/H1N1 2009 in Thailand: An Experience with Better<br />
Test Performance in Resource Limited Setting<br />
Source : Journal <strong>of</strong> Infection, Volume 60, Issue 1, January <strong>2010</strong>, Pages 86-87<br />
Document Type : Letter<br />
601
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Keywords : adolescent, adult, aged, child, controlled study, diagnostic accuracy, diagnostic test, diagnostic value, epidemic, female, high<br />
risk population, human, infant, infection risk, influenza, Influenza virus A H1N1, intermethod comparison, letter, major clinical<br />
study, male, nose smear, outpatient care, reverse transcription polymerase chain reaction, sensitivity and<br />
specificity, Thailand, throat culture, virus detection, virus identification, virus transmission;<br />
Adolescent, Adult, Aged, Child, Child, Preschool, Diagnosis, Differential, Disease Outbreaks, Female, Humans, Infant, Influenza<br />
A Virus, H1N1 Subtype, Influenza, Human, Male, Microbiological Techniques, Middle Aged, Sensitivity and<br />
Specificity, Thailand, Young Adult<br />
<strong>Abstract</strong> : –<br />
No.1650<br />
Author (s) : Watcharananan, S.P., Junchotikul, P., Srichanrusmi, C., Chanchompoo, P., Mavichak, V., Kantachuvessiri, S., Chantratita, W.<br />
Title : Adenovirus infection after kidney transplantation in Thailand: Seasonal distribution and potential route <strong>of</strong> acquisition<br />
Source : Transplantation Proceedings, Volume 42, Issue 10, December <strong>2010</strong>, Pages 4091-4093<br />
Document Type : Conference Paper<br />
Keywords : adenovirus infection, adult, clinical article, conference paper, hospital infection, human, kidney donor, kidney<br />
transplantation, living donor, medical record review, nose smear, priority journal, real time polymerase chain<br />
reaction, retrospective study, seasonal variation, symptom, Thailand, urinalysis, virus transmission<br />
<strong>Abstract</strong> : Background Data on the epidemiology and route <strong>of</strong> acquisition <strong>of</strong> adenovirus (ADV) infection after kidney transplantation are<br />
limited. From April 2007 to March <strong>2010</strong>, there were 17 cases <strong>of</strong> ADV infection: namely, 2 from April to December 2007; 8 from<br />
January to December 2008; 4 from January to December 2009; and 3 from January to March <strong>2010</strong>. Most cases occurred in<br />
October and November (n = 8; 47.1%), followed by February to April (n = 6; 35.3%) and July (n = 3; 17.6%). Methods From<br />
April 2007 to August 2009, the diagnosis <strong>of</strong> ADV infection was made based on patient symptoms. From September 2009 to<br />
March <strong>2010</strong>, in addition to symptoms, the diagnosis was complemented by urine surveillance for ADV using real-time<br />
polymerase chain reaction (PCR) prospectively performed every 12 weeks among recipients <strong>of</strong> living-related kidney, starting at<br />
week 2 posttransplantation for a total <strong>of</strong> 812 weeks. Before transplantation, recipients and donors were screened for ADV in<br />
urine and also using nasal swab. Results Only 1 <strong>of</strong> the 24 patients displayed a positive ADV PCR in the urine surveillance<br />
study. A local investigation during a cluster <strong>of</strong> cases in October 2008 showed 2 patients who developed ADV after sharing a<br />
room in the transplant unit. Although nosocomial transmission was probable, the majority <strong>of</strong> cases were scattered over time<br />
rather than clustering in 1 time period. Conclusion These findings suggested that ADV infection cases occurred after<br />
exogenous exposure. In a resource-limited country, early diagnosis <strong>of</strong> ADV is justified for patients with compatible symptoms<br />
complemented by intense infection control to prevent nosocomial transmission from a confirmed case. © <strong>2010</strong> Elsevier Inc.<br />
All rights reserved.<br />
No.1651<br />
Author (s) : Watcharananan, S.P., Kiertiburanakul, S., Piyatuctsanawong, W., Anurathapan, U., Sungkanuparph, S., Pakakasama, S.,<br />
Chantratita, W., Hongeng, S.<br />
Title : Cytomegalovirus, adenovirus, and polyomavirus co-infection among pediatric recipients <strong>of</strong> allogeneic stem cell<br />
transplantation: Characteristics and outcome<br />
Source : Pediatric Transplantation, Volume 14, Issue 5, August <strong>2010</strong>, Pages 675-681<br />
Document Type : Article<br />
Keywords : allogeneic; hematopoietic stem cell transplantation; pediatric; viral co-infection<br />
<strong>Abstract</strong> : ADV and PMV infection have increasingly been documented as significant complications following allo-HSCT. Despite<br />
increasing recognition, characteristics and outcome <strong>of</strong> CMV, ADV, and PMV viral co-infection remain obscured. In this study, a<br />
retrospective quantitative PCR analysis <strong>of</strong> ADV, PMV (BKV and JCV) was performed from pediatric patients' stored blood<br />
samples previously tested for CMV viremia after allo-HSCT. Clinical and virological characteristics and outcome among<br />
patients with and without viral co-infection were analyzed and compared. From 2001 to 2006, 219 blood samples from 69<br />
patients were studied. Viral DNA was present in 119 samples (52.9%).The proportion <strong>of</strong> viremia was highest for BKV (30.6%),<br />
followed by CMV (20.9%), ADV (9.1%), and JCV (0.5%). Viral co-infection occurred in 17 patients (24.6%), with CMV/BKV as the<br />
most common type (11.6%), followed by CMV/ADV (4.3%) and ADV/BKV (2.9%). From multivariate analysis, factors associated<br />
with viral co-infection were acute GVHD (OR 4.57; 95% CI 1.9-10.96, p = 0.001), level <strong>of</strong> blood CMV viral load (OR 1.53; 95% CI<br />
1.24-1.89, p < 0.001), and level <strong>of</strong> blood ADV viral load (OR 1.56; 95% CI 1.05-2.32, p = 0.027). Higher probability <strong>of</strong> developing<br />
viral disease was strongly associated with more types <strong>of</strong> virus detected in blood (p < 0.001). Significant difference in the<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
causes <strong>of</strong> death was observed among patients with and without viral co-infection (p = 0.014). Infection (87.5%) was the major<br />
cause <strong>of</strong> death <strong>of</strong> patients with viral co-infection, whereas relapse <strong>of</strong> hematologic disease (70%) was the major cause <strong>of</strong> death<br />
<strong>of</strong> patients with mono-viral infection. Viral co-infection is a common and significant infectious complication in pediatric<br />
recipients <strong>of</strong> allo-HSCT. Blood monitoring <strong>of</strong> CMV, ADV, and BKV is suggested among pediatric patients who develop GvHD or<br />
who have rising <strong>of</strong> CMV or ADV viremia following allo-HSCT. © <strong>2010</strong> John Wiley & Sons A/S.<br />
No.1652<br />
Author (s) : Watcharananan, S.P., Suwatanapongched, T., Wacharawanichkul, P., Chantratitaya, W., Mavichak, V., Mossad, S.B.<br />
Title : Influenza AH1N1 2009 pneumonia in kidney transplant recipients: Characteristics and outcomes following high-dose<br />
oseltamivir exposure<br />
Source : Transplant Infectious Disease, Volume 12, Issue 2, April <strong>2010</strong>, Pages 127-131<br />
Document Type : Article<br />
Keywords : H1N1; Influenza A; Kidney transplant; Oseltamivir; Pneumonia<br />
<strong>Abstract</strong> : We report 2 cases <strong>of</strong> severe pneumonia due to the novel pandemic influenza AH1N1 2009 in kidney transplant recipients.<br />
Our patients initially experienced influenza-like illness that rapidly progressed to severe pneumonia within 48 h. The patients<br />
became hypoxic and required non-invasive ventilation. The novel influenza AH1N1 2009 was identified from their nasal<br />
swabs. These cases were treated successfully with a relatively high dose <strong>of</strong> oseltamivir, adjusted for their renal function.<br />
Clinical improvement was documented only after a week <strong>of</strong> antiviral therapy. Despite early antiviral treatment, we showed<br />
that morbidity following novel pandemic influenza AH1N1 2009 infection is high among kidney transplant recipients. © <strong>2010</strong><br />
John Wiley & Sons A/S.<br />
No.1653<br />
Author (s) : Watcharasamphankul, W.; Foongladda, S.<br />
Title : Multiprobes real-time PCR direct detection <strong>of</strong> MDR-TB related genes in pulmonary samples<br />
Source : INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, Volume: 14, Supplement: 1, Pages: E370-E370, DOI:<br />
10.1016/j.ijid.<strong>2010</strong>.02.444, Published: MAR <strong>2010</strong><br />
Document Type : Meeting Abdtract<br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1654<br />
Author (s) : Watcharasit, P., Suntararuks, S., Visitnonthachai, D., Thiantanawat, A., Satayavivad, J.<br />
Title : Acrylonitrile induced apoptosis via oxidative stress in neuroblastoma SH-SY5Y cell<br />
Source : Journal <strong>of</strong> Applied Toxicology, Volume 30, Issue 7, October <strong>2010</strong>, Pages 649-655<br />
Document Type : Article<br />
Keywords : Acrylonitrile; Apoptosis; Oxidative stress<br />
<strong>Abstract</strong> : Acrylonitrile (ACN) is a chemical that is widely used in the production <strong>of</strong> plastics, acrylic fibers, synthetic rubbers and resins. It<br />
has been reported that ACN can cause oxidative stress, a condition which is well recognized as an apoptotic initiator;<br />
however, information regarding ACN-induced apoptosis is limited. This present study investigated whether ACN induces<br />
apoptosis in human neuroblastoma SH-SY5Y cells, and whether its apoptotic induction involves oxidative stress. The results<br />
showed that ACN caused activation <strong>of</strong> caspase-3, a key enzyme involved in apoptosis, in a dose- and time-dependent<br />
manner. Detection <strong>of</strong> sub-G1 apoptotic cell death and apoptotic nuclear condensation revealed that ACN caused an increase<br />
in the number <strong>of</strong> apoptotic cells indicating ACN induces apoptosis in SH-SY5Y cells. ACN dose- and time-dependently<br />
increased the level <strong>of</strong> proapoptotic protein, Bax. Pretreatment with N-acetylcysteine (NAC), an antioxidant, attenuated<br />
caspase-3 activation by ACN, as evidenced by a reduction in proteolysis <strong>of</strong> PARP, a known caspase-3 substrate, as well as in<br />
the number <strong>of</strong> sub-G1 apoptotic cells. Moreover, induction <strong>of</strong> Bax by ACN was abolished by NAC. Taken together, the results<br />
indicate that ACN induces apoptosis in SH-SY5Y cells via a mechanism involving generation <strong>of</strong> oxidative stress-mediated Bax<br />
induction. Copyright © <strong>2010</strong> John Wiley & Sons, Ltd.<br />
No.1655<br />
Author (s) : Watcharatanyatip, K., Boonmoh, S., Chaichoun, K., Songserm, T., Woratanti, M., Dharakul, T.<br />
Title : Multispecies detection <strong>of</strong> antibodies to influenza A viruses by a double-antigen sandwich ELISA<br />
603
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Source : Journal <strong>of</strong> Virological Methods, Volume 163, Issue 2, February <strong>2010</strong>, Pages 238-243<br />
Document Type : Article<br />
Keywords : Antibody detection; Double-antigen sandwich ELISA; Influenza virus; Nucleoprotein<br />
<strong>Abstract</strong> : A double-antigen sandwich ELISA was developed for the detection <strong>of</strong> antibodies to influenza A viruses. A recombinant<br />
nucleoprotein (rNP) <strong>of</strong> influenza A virus was used as a capture antigen and an HRP-conjugate for detecting the antibodies. A<br />
total <strong>of</strong> 125 serum samples from birds <strong>of</strong> different species including chickens, geese, open-billed storks, Khaki Campbell<br />
ducks, lesser whistling ducks, and pigeons with known antibodies were tested by ELISA. The sensitivity and the specificity <strong>of</strong><br />
ELISA were found to be 98% and 97.3%, respectively. The assay was able to detect the presence <strong>of</strong> influenza A antibodies as<br />
early as the fourth day post-inoculation in ducks infected experimentally with influenza A (H5N1) virus. Excellent agreement<br />
(97.6%) was obtained between this sandwich ELISA and the hemagglutination inhibition (HI) tests (κ = 0.95). The doubleantigen<br />
sandwich ELISA correlated well with a commercial avian influenza (AI) multispecies ELISA and was slightly more<br />
sensitive than the AI multispecies ELISA. These findings indicate that the double-antigen sandwich ELISA based on rNP may<br />
<strong>of</strong>fer an effective screening method for serodiagnosis <strong>of</strong> influenza A virus. The double-antigen sandwich ELISA also enables<br />
the detection <strong>of</strong> antibodies to influenza A viruses in different species without the need for species-specific secondary<br />
antibodies. © 2009 Elsevier B.V. All rights reserved.<br />
No.1656<br />
Author (s) : Wattanakorn, N., Asavapichayont, P., Nunthanid, J., Limmatvapirat, S., Sungthongjeen, S., Chantasart, D., Sriamornsak, P.<br />
Title : Pectin-based bioadhesive delivery <strong>of</strong> carbenoxolone sodium for aphthous ulcers in oral cavity<br />
Source : AAPS PharmSciTech, Volume 11, Issue 2, June <strong>2010</strong>, Pages 743-751<br />
Document Type : Article<br />
Keywords : Aphthous ulcers; Bioadhesive drug delivery; Buccal; Carbenoxolone sodium; Pectin<br />
<strong>Abstract</strong> : The objective <strong>of</strong> this study was to prepare and evaluate the pectin-based dosage form for buccal adhesion. Carbenoxolone<br />
sodium, which is used for the treatment <strong>of</strong> aphthous ulcers in oral cavity, was used as a model drug. The pectin buccal discs<br />
were prepared by direct compression. The water uptake and erosion <strong>of</strong> pectin disc increased progressively with the swelling<br />
time. The bioadhesion <strong>of</strong> dried pectin discs decreased when either the discs were hydrated or the buccal tissue was wet with<br />
a small volume <strong>of</strong> medium. The influencing factors such as pectin type, pectin to lactose ratio, and sweetener type on the<br />
formulations were investigated. The results demonstrated that buccal discs prepared from pectin with a high degree <strong>of</strong><br />
esterification (DE) showed a weaker and more friable characteristic than that with low DE. Decreasing pectin to lactose ratio<br />
resulted in the high dissolution rate with low bioadhesive properties. Addition <strong>of</strong> sweetener in the formulations also affected<br />
the hardness, friability, and bioadhesive properties <strong>of</strong> the discs. The pectin discs containing sweetening agent showed a higher<br />
drug release than those without sweetener. The results suggested that pectin-based bioadhesive discs could be used to<br />
deliver carbenoxolone sodium in oral cavity. © <strong>2010</strong> American Association <strong>of</strong> Pharmaceutical Scientists.<br />
No.1657<br />
Author (s) : Wattanakrai, P., Rojhirunsakool, S., Pootongkam, S.<br />
Title : Split-face comparison <strong>of</strong> long-pulse-duration neodymium-doped yttrium aluminum garnet (Nd:YAG) 1,064-nm laser alone and<br />
combination long-pulse and Q-switched Nd:YAG 1,064-nm laser with carbon photoenhancer lotion for the treatment <strong>of</strong><br />
enlarged pores in asian women<br />
Source : Dermatologic Surgery, Volume 36, Issue 11, November <strong>2010</strong>, Pages 1672-1680<br />
Document Type : Article<br />
Keywords : carbon, steroid, charcoal, photosensitizing agent; acne, adult, article, comparative<br />
study, erythema, female, human, hypopigmentation, lotion, neodymium laser, normal human, papular<br />
rash, photography, physician, priority journal, pruritus, treatment duration, urticaria, Asian, clinical trial, controlled clinical<br />
trial, controlled study, esthetic surgery, laser, low level laser therapy, middle aged, radiation exposure, randomized controlled<br />
trial, skin, solid state laser, topical drug administration; Administration, Topical, Adult, Asian Continental Ancestry<br />
Group, Charcoal, Cosmetic Techniques, Female, Humans, Laser Therapy, Low-Level, Lasers, Lasers, Solid-State, Middle<br />
Aged, Photosensitizing Agents, Skin<br />
<strong>Abstract</strong> : BACKGROUND: Long-pulse and Q-switched neodymium-doped yttrium aluminum garnet (Nd:YAG) 1,064-nm laser used for<br />
facial rejuvenation can improve pore size. Topical carbon has been used to enhance efficacy. OBJECTIVE: To compare the<br />
efficacy and safety <strong>of</strong> a 1,064-nm long-pulse Nd:YAG laser alone with that <strong>of</strong> a combination Q-switched Nd:YAG laser with<br />
topical carbon lotion followed by long-pulse Nd:YAG to improve enlarged pores. METHODS: Twenty Thai women randomly<br />
604
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
received five treatments with a long-pulse Nd:YAG laser on one facial half (LP side) and long-pulse Nd:YAG after carbonassisted<br />
Q-switched Nd:YAG laser on the contralateral side (carbon QS+LP side) at 2-week intervals. Participants were<br />
evaluated using digital photography, complexion analysis, and a chromometer. RESULTS: There was significant decrease in<br />
pore counts <strong>of</strong> 35.5% and 33% from baseline on the carbon QS+LP and LP sides, respectively. Physician-evaluated pore size<br />
improvement was 67% on the carbon QS+LP sides and 60% on the LP sides. Chromometer measurement showed an<br />
increase in skin lightness index. There was no significant difference between the two treatments, although there were more<br />
adverse effects on the carbon QS+LP side. CONCLUSION: Long-pulse Nd:YAG 1,064-nm laser improves the appearance <strong>of</strong><br />
facial pores and skin color. Adding carbon-assisted Q-switched Nd:YAG did not enhance the results but produced more side<br />
effects. Laser Engineer Co. Ltd, Bangkok, Thailand loaned and supplied the laser machine and topical carbon used in this<br />
study. © <strong>2010</strong> by the American Society for Dermatologic Surgery, Inc.<br />
No.1658<br />
Author (s) : Wattanakrai, P., Temnithikul, B., Pootongkam, S.<br />
Title : Pattern <strong>of</strong> corticosteroid allergy in Thailand<br />
Source : Dermatitis, Volume 21, Issue 4, August <strong>2010</strong>, Pages 203-206<br />
Document Type : Article<br />
Keywords : alclometasone dipropionate, betamethasone valerate, budesonide, clobetasol, hydrocortisone butyrate, tixocortol<br />
pivalate, triamcinolone acetonide; adult, allergic reaction, allergy, article, drug screening, human, major clinical study, patch<br />
test, prevalence, priority journal, skin allergy, Thailand; Adrenal Cortex Hormones, Adult, Budesonide, Dermatitis, Allergic<br />
Contact, Humans, Hydrocortisone, Middle Aged, Patch Tests, Prevalence, Retrospective Studies, Thailand, Young Adult<br />
<strong>Abstract</strong> : Background: Corticosteroid allergy is a complication <strong>of</strong> topical therapy detected by patch-testing with corticosteroid allergens.<br />
Objective and Methods: Ten-year retrospective review to study the prevalence and patterns <strong>of</strong> corticosteroid allergy in Thai<br />
patients. Results: Of 882 patients who were patch-tested, 29 (3.29%) had allergic reactions to corticosteroids. Of these 29<br />
patients, 17 (58.62%) had positive reactions to one corticosteroid, and 12 (41.38%) reacted to multiple corticosteroids. Rates<br />
<strong>of</strong> reaction to corticosteroid groups ranged from 31.03 to 80.95%. Concomitant reactions between groups were noted. The<br />
prevalence <strong>of</strong> topical corticosteroid allergy (using two screening allergens, tixocortol pivalate and budesonide) was 2.27% (20<br />
<strong>of</strong> 882). Testing with additional steroid allergens in suspected cases increased the prevalence to 3.29%. Tixocortol pivalate<br />
detected 51.72% <strong>of</strong> corticosteroid- allergic cases, and budesonide detected 24.14%. Combining both tixocortol and<br />
budesonide detected 68.97% <strong>of</strong> cases. Conclusion: Corticosteroid allergy is found to multiple corticosteroids, and<br />
concomitant reactions occur across groups. Group D1 corticosteroid esters produced a higher positive reaction rate (61.9%)<br />
than groups D2 (52.38%) and A (51.72%). This may be due to different prescribing habits or the easy access to D1<br />
corticosteroids sold over the counter by pharmacies in Thailand. © <strong>2010</strong> American Contact Dermatitis Society. All Rights<br />
Reserved.<br />
No.1659<br />
Author (s) : Wattanakrai, P., Mornchan, R., Eimpunth, S.<br />
Title : Low-Fluence Q-Switched Neodymium-Doped Yttrium Aluminum Garnet (1,064 nm) Laser for the Treatment <strong>of</strong> Facial Melasma<br />
in Asians<br />
Source : DERMATOLOGIC SURGERY, Volume: 36, Issue: 1, Pages: 76-87, DOI: 10.1111/j.1524-4725.2009.01383.x, Published: JAN <strong>2010</strong><br />
Document Type : Article<br />
Keywords : ND-YAG LASER; INTENSE PULSED-LIGHT; REFRACTORY MELASMA; SELECTIVE PHOTOTHERMOLYSIS; ALEXANDRITE LASER; RUBY-<br />
LASER; SKIN COLOR; IN-VIVO; EXPRESSION; CO2-LASER<br />
<strong>Abstract</strong> : BACKGROUND: Pigment lasers have been used in melasma with unsatisfactory results. OBJECTIVE: To determine the<br />
effectiveness and safety <strong>of</strong> 1,064-nm Q-switched neodymium-doped yttrium aluminum garnet (QS-Nd:YAG) laser treatment <strong>of</strong><br />
melasma in Asians. MATERIALS AND METHODS: Split-face randomized study comparing combination QS-Nd:YAG laser and 2%<br />
hydroquinone with topical treatment in dermal or mixed-type melasma. Twenty-two patients were treated with 1,064-nm QS-<br />
Nd:YAG laser, 6-mm spot size, 3.0- to 3.8-J/cm2 fluence for five sessions at 1-week intervals. Pigmentation was objectively<br />
recorded using a colorimeter (lightness index score), and subjective assessments were evaluated using the modified Melasma<br />
Area and Severity Index (mMASI) score. RESULTS: After five laser treatments, statistically significant improvement <strong>of</strong> melasma<br />
from baseline was observed in colorimeter (p
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
22 patients developed rebound hyperpigmentation, and all patients had recurrence <strong>of</strong> melasma. CONCLUSION: QS-Nd:YAG<br />
laser treatment for melasma in Asians produced only temporary improvement and had side effects. Common complications<br />
were hypopigmentation, melasma recurrence, and rebound hyperpigmentation. The authors have indicated no significant<br />
interest with commercial supporters.<br />
No.1660<br />
Author (s) : Wattanasiriwech, S., Wattanasiriwech, D., Svasti, J.<br />
Title : Production <strong>of</strong> amorphous silica nanoparticles from rice straw with microbial hydrolysis pretreatment<br />
Source : Journal <strong>of</strong> Non-Crystalline Solids, Volume 356, Issue 25-27, 1 June <strong>2010</strong>, Pages 1228-1232<br />
Document Type : Article<br />
Keywords : Amorphous silica; Enzymatic hydrolysis; Microbial community; Rice straw; Trichoderma reesei<br />
<strong>Abstract</strong> : An enzymatic hydrolysis approach was used in order to develop a less aggressive process for production <strong>of</strong> amorphous silica<br />
from rice straw. In the process, rice straw was firstly hydrolyzed either with a microbial isolate (Trichoderma reesei TISTR 3080)<br />
or a microbial community (LDD1), followed by a heat treatment at 500 °C for 8 h. After hydrolysis, the rice straw was<br />
decomposed by T. reesei and by LDD1 to 59.6% and 45.2% <strong>of</strong> the initial weight, <strong>of</strong> which 12% and 23% ash content was<br />
respectively determined by thermogravimetric analysis. X-ray diffraction and transmission electron microscopy analysis<br />
confirmed that the major constituent in the ash to be amorphous silica with a particle size ranges between 50 and 80 nm.<br />
The silica content in the T. reesei pretreated ash was 82.5%, whereas the content <strong>of</strong> the LDD1 pretreated ash was 73.6%.<br />
Relatively high levels <strong>of</strong> impurities such as manganese and phosphate, associated with microbial activity were detected in<br />
both pretreated ash samples. © <strong>2010</strong> Elsevier B.V. All rights reserved.<br />
No.1661<br />
Author (s) : Watthanachaiyingcharoen, R., Komatsu, K., Zhu, S., Vajragupta, O., Leelamanit, W.<br />
Title : Authentication <strong>of</strong> Coscinium fenestratum among the other Menispermaceae plants prescribed in Thai folk medicines<br />
Source : Biological and Pharmaceutical Bulletin, Volume 33, Issue 1, January <strong>2010</strong>, Pages 91-94<br />
Document Type : Article<br />
Keywords : 18S ribosomal RNA gene; Arcangelisia flava; Coscinium fenestratum; Fibraurea tinctoria; Internal transcribed spacer<br />
gene; Polymerase chain reaction-restriction fragment length polymorphism<br />
<strong>Abstract</strong> : In Ayurveda and Thai traditional medicines, material from Coscinium fenestratum is commonly prescribed as active<br />
ingredients with diverse therapeutic purposes. However, C. fenestratum is also a seriously endangered medicinal liana. Thus,<br />
its crude material is very rare and is being substituted with substances from Arcangelisia flava or Fibraurea tinctoria<br />
(Menispermaceae), which have high morphological similarity. In this current study, nuclear 18S ribosomal RNA (rRNA) gene and<br />
nuclear ribosomal DNA internal transcribed spacer (ITS) gene sequences with the polymerase chain reaction-restriction<br />
fragment length polymorphisms (PCR-RFLPs) technique were exploited to identify these three species. The nuclear 18S rRNA<br />
gene sequences <strong>of</strong> C. fenestratum, A. flava, and F. tinctoria consisted <strong>of</strong> 1809, 1805, and 1809 base pairs (bps), respectively,<br />
while their ITS gene regions were 694, 622, and 631 bps in length, respectively. The 18S rRNA gene <strong>of</strong> C. fenestratum digested<br />
with SmaI restriction enzyme displayed the electrophoresis pr<strong>of</strong>ile <strong>of</strong> 729 and 790 bps; for A. flava and F. tinctoria, the<br />
digested products showed fragments <strong>of</strong> 1519 bps. Although the ITS gene regions <strong>of</strong> A. flava and F. tinctoria had unrecognized<br />
sequences with SalI, the SalI-digested ITS <strong>of</strong> C. fenestratum exhibited fragments <strong>of</strong> approximately 599 bp. Thus, the 18S rRNA<br />
gene and ITS gene sequences with PCR-RFLPs were proven to be powerful molecular markers for identifying C. fenestratum<br />
and distinguishing it from the other two Menispermaceae plants. © <strong>2010</strong> Pharmaceutical Society <strong>of</strong> Japan.<br />
No.1662<br />
Author (s) : Watthanakulpanich, D., Waikagul, J., Maipanich, W., Nuamtanong, S., Sanguankiat, S., Pubampen, S., Praevanit, R.,<br />
Mongkhonmu, S., Nawa, Y.<br />
Title : Haplorchis taichui as a possible etiologic agent <strong>of</strong> irritable bowel syndrome-like symptoms<br />
Source : Korean Journal <strong>of</strong> Parasitology, Volume 48, Issue 3, September <strong>2010</strong>, Pages 225-229<br />
Document Type : Article<br />
Keywords : Clinical feature; Haplorchis taichui; Irritable bowel syndrome-like symptom<br />
<strong>Abstract</strong> : The aim <strong>of</strong> this study is to clarify the clinical features <strong>of</strong> Haplorchis taichui infection in humans in Nan Province, Thailand, and<br />
to correlate the clinical features with irritable bowel syndrome (IBS)-like symptoms. In this study area, only H. taichui, but<br />
neither other minute intestinal flukes nor small liver flukes were endemic. The degree <strong>of</strong> infection was determined by fecal<br />
606
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
egg counts and also by collecting adult worms after deworming. The signs and symptoms <strong>of</strong> individual patients together with<br />
their hematological and biochemical laboratory data were gathered to evaluate the relationship between the clinical features<br />
and the severity <strong>of</strong> infection. Special emphasis was made to elucidate the possible similarities <strong>of</strong> the clinical features <strong>of</strong> H.<br />
taichui infection and IBS-like symptoms. The results showed useful clinical information and the significant (> 50%) proportion<br />
<strong>of</strong> haplorchiasis patients complained <strong>of</strong> abdominal pain, lassitude, and flatulence, which were the important diagnostic<br />
symptoms <strong>of</strong> IBS. This study has reported a possible link between H. taichui and IBS, and H. taichui might probably play a role<br />
in the etiology <strong>of</strong> these IBS-like symptoms.<br />
No.1663<br />
Author (s) : Watthanawisuth, N., Tongrod, N., Kerdcharoen, T., Tuantranont, A.<br />
Title : Real-time monitoring <strong>of</strong> GPS-tracking tractor based on ZigBee multi-hop mesh network<br />
Source : ECTI-CON <strong>2010</strong> - The <strong>2010</strong> ECTI <strong>International</strong> Conference on Electrical Engineering/Electronics, Computer,<br />
Telecommunications and Information Technology, <strong>2010</strong>, Article number 5491422, Pages 580-583<br />
Document Type : Conference Paper<br />
Keywords : Mesh topologies, Multi-hop mesh networks, Precision Agriculture, Real time, Real time monitoring, Tracking<br />
system, Transmission <strong>of</strong> data, Zig-Bee; Information technology, Routers, Tractors (agricultural), Tractors (truck); Global<br />
positioning system<br />
<strong>Abstract</strong> : This research is a part <strong>of</strong> smart farm system in the framework <strong>of</strong> precision agriculture. The system was installed and tested<br />
over a year. The tractor tracking system employs the Global Positioning System (GPS) and ZigBee wireless network based on<br />
mesh topology to make the system communicate covering a large area. Router nodes are used for re-transmission <strong>of</strong> data in<br />
the network. A s<strong>of</strong>tware was developed for acquiring data from tractor, storing data and displaying in real time on a web site.<br />
No.1664<br />
Author (s) : Weerachayaphorn, J., Chuncharunee, A., Jariyawat, S., Lewchalermwong, B., Amonpatumrat, S., Suksamrarn, A., Piyachaturawat,<br />
P.<br />
Title : Protection <strong>of</strong> centrilobular necrosis by Curcuma comosa Roxb. in carbon tetrachloride-induced mice liver injury<br />
Source : Journal <strong>of</strong> Ethnopharmacology, Volume 129, Issue 2, May <strong>2010</strong>, Pages 254-260<br />
Document Type : Article<br />
Keywords : Carbon tetrachloride; Curcuma comosa; Glutathione-S-transferase; Hepatic detoxifying system; Hepatoprotection<br />
<strong>Abstract</strong> : Aim <strong>of</strong> the study: To investigate the protective effect and possible mechanism <strong>of</strong> Curcuma comosa hexane extract on CCl4induced<br />
liver injury in adult male mice. Materials and methods: Hepatotoxicity was induced by an intraperitoneal injection <strong>of</strong><br />
CCl4 and was evaluated after 24h from the elevations <strong>of</strong> plasma alanine transaminase (ALT) and aspartate transaminase (AST)<br />
activities, and histological analysis <strong>of</strong> liver injuries. Hexane extract <strong>of</strong> Curcuma comosa was given at different time points from<br />
1 to 72h, prior to CCl4 administration and the protection from liver injury was assessed. Results: CCl4-induced damage to liver<br />
cells was resulted in elevations <strong>of</strong> plasma ALT and AST activities. Pretreatment with Curcuma comosa hexane extract 24h at a<br />
dose <strong>of</strong> 100, 250, and 500mg/kgBW resulted in a dose-dependent prevention <strong>of</strong> the increases in plasma ALT and AST<br />
activities as well as time dependent. The protective effect <strong>of</strong> the extract at a dose <strong>of</strong> 500mg/kgBW was seen at 12-24h.<br />
Pretreatment <strong>of</strong> the extract completely prevented elevation <strong>of</strong> plasma ALT and AST activities, and centrilobular necrosis. The<br />
protective effect <strong>of</strong> Curcuma comosa was associated with restoration <strong>of</strong> hepatic glutathione content, and CYP2E1 catalytic<br />
activity, and its mRNA and protein levels as well as increase in activity <strong>of</strong> glutathione-S-transferase (GST). Conclusion: Curcuma<br />
comosa has a potent protective property against CCl4-induced hepatic injuries via the activation <strong>of</strong> detoxifying mechanisms<br />
(GST) as well as reduction <strong>of</strong> the bioactive toxic metabolites. Therefore, Curcuma comosa may be beneficial for prevention <strong>of</strong><br />
hepatotoxicity. © <strong>2010</strong> Elsevier Ireland Ltd.<br />
No.1665<br />
Author (s) : Werarak, P., Kiratisin, P., Thamlikitkul, V.<br />
Title : Hospital-acquired pneumonia and ventilator-associated pneumonia in adults at Siriraj Hospital: etiology, clinical outcomes,<br />
and impact <strong>of</strong> antimicrobial resistance.<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand = Chotmaihet thangphaet, Volume 93 Suppl 1, January <strong>2010</strong>, Pages S126-138<br />
Document Type : Article<br />
Keywords : antiinfective agent; aged, article, cross infection, drug effect, female, Gram negative bacterium, Gram positive<br />
bacterium, human, isolation and purification, length <strong>of</strong> stay, male, microbiology, middle aged, mortality, multidrug<br />
607
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
resistance, pneumonia, prevalence, prospective study, risk factor, Thailand, treatment outcome, ventilator, ventilator<br />
associated pneumonia; Aged, Aged, 80 and over, Anti-Bacterial Agents, Cross Infection, Drug Resistance, Multiple,<br />
Bacterial, Female, Gram-Negative Bacteria, Gram-Positive Bacteria, Humans, Length <strong>of</strong> Stay, Male, Middle<br />
Aged, Pneumonia, Pneumonia, Ventilator-Associated, Prevalence, Prospective Studies, Risk Factors, Thailand, Treatment<br />
Outcome, Ventilators, Mechanical<br />
<strong>Abstract</strong> : BACKGROUND: Nosocomial pneumonia (NP), hospital-acquired pneumonia (HAP) and ventilator-associated pneumonia (VAP), is<br />
an important cause <strong>of</strong> morbidity and mortality in hospitalized patients. One <strong>of</strong> the factors contributing to a high mortality rate<br />
<strong>of</strong> HAP and VAP could be antibiotic resistance among the causative agents. OBJECTIVE: To determine prevalence <strong>of</strong> bacterial<br />
pathogens clinical features, risk factors <strong>of</strong> HAP and VAP, antimicrobial resistance among major respiratory pathogens, clinical<br />
implication <strong>of</strong> antimicrobial resistance, antimicrobial regimens used, and treatment outcomes <strong>of</strong> adult patients with HAP and<br />
VAP at Siriraj Hospital. MATERIAL AND METHOD: This was a prospective, hospital-based, active surveillance study on HAP and<br />
VAP in hospitalized adults at Siriraj Hospital from December 2007 to March 2009. The patients with HAP and VAP were<br />
followed prospectively until they expired or were discharged from the hospital. RESULTS: One hundred and forty-six adult<br />
patients were included. Seventy percent <strong>of</strong> the patients were males with the mean age <strong>of</strong> 70.8 years. HAP was accounted for<br />
24.7% and VAP 75.3%. Most <strong>of</strong> the patients (82.9%) had late-onset HAP or VAP with the median day <strong>of</strong> onset <strong>of</strong> pneumonia<br />
<strong>of</strong> 11 days. Two third <strong>of</strong> the patients were hospitalized in general medical wards. Bronchopneumonia was observed in 53.4%<br />
and multilobar pneumonia in 24.7%. A. baumanni was the most common isolated pathogen and 92.3% <strong>of</strong> them were<br />
multidrug-resistant (MDR) or pandrug-resistant (PDR). The other common isolated pathogens were K. pneumoniae, P.<br />
aeruginosa and methicillin-resistant S. aureus (MRSA). Carbapenem was the most commonly used initial antibiotic (45.9%)<br />
followed by colistin (21.9%) and cephalosporins (21.1%). The concordance <strong>of</strong> initial antibiotics was 58.9%. Antibiotics were<br />
modified 43.8% <strong>of</strong> the patients. Colistin was the most commonly used modified antibiotic followed by carbapenem. The<br />
modified antibiotics were concordant with isolated bacteria in 98.4%. The patients received mechanical ventilators in 81.5%<br />
with the median ventilator day <strong>of</strong> 10 days. At the initial response (72 hours after antibiotic therapy), an improvement was<br />
56.8% and a mortality rate due to pneumonia was 14.4%. Death due to pneumonia at the end <strong>of</strong> treatment was 42.5%. The<br />
30-day mortality from pneumonia was 45.9%. There were no significant differences in the outcomes <strong>of</strong> pneumonia between<br />
HAP and VAP. The factors associated with PDR-organisms were late-onset hospital-acquired pneumonia and previous<br />
carbapenem usage within 72 hours. Septic shock and bilateral lung involvement were significantly associated with<br />
unfavorable outcomes at 72 hours. Septic shock, severe sepsis, and previous carbapenem usage within 72 hours were<br />
significantly associated with mortality at the end <strong>of</strong> treatment and at 30 days after developing pneumonia. CONCLUSION: HAP<br />
and VAP remain to be very important hospital-acquired infections at Siriraj Hospital. The isolated pathogens are usually<br />
multidrug-resistant and the mortality rate remains high. The local data on prevalence <strong>of</strong> the isolated pathogens and their<br />
antibiotic susceptibility may help clinicians choose more appropriate initial antibiotics in order to improve the outcome and<br />
to decrease the emergence <strong>of</strong> resistant organisms.<br />
No.1666<br />
Author (s) : Werayingyong, P.; Phanuphak, N.; Chokephaibulkit, K.; Kullert, N.; Tosanguan, K.; Butchon, R.; Voramongkol, N.; Boonsuk, S.;<br />
Teerawattananon, Y.<br />
Title : ECONOMIC EVALUATION OF THREE-DRUG ANTIRETROVIRAL REGIMENS FOR THE PREVENTION OF MOTHER-TO-CHILD<br />
TRANSMISSION OF HIV IN THAILAND<br />
Source : VALUE IN HEALTH, Volume: 13, Issue: 7, Pages: A550-A550, Published: NOV <strong>2010</strong><br />
Document Type : Meeting Abdtract<br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1667<br />
Author (s) : Wertheim, H.F.L., Puthavathana, P., Nghiem, N.M., Rogier van Doorn, H., Nguyen, T.V., Pham, H.V., Subekti, D., Harun, S., Malik,<br />
S., Robinson, J., Rahman, M., Taylor, W., Lindegardh, N., Wignall, S., Farrar, J.J., de Jong, M.D.<br />
Title : Laboratory capacity building in Asia for infectious disease research: Experiences from the South East Asia Infectious Disease<br />
Clinical Research Network (SEAICRN)<br />
Source : PLoS Medicine, Volume 7, Issue 4, April <strong>2010</strong><br />
Document Type : Article<br />
608
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Keywords : GCLP, good clinical laboratory practice; MDL, molecular diagnostic laboratory; pH1N1, influenza A (H1N1); RT-PCR, reverse<br />
transcriptase polymerase chain reaction; SEAICRN, South East Asia Infectious Disease Clinical Research Network; SOP,<br />
standard operating procedure<br />
<strong>Abstract</strong> : Enhancing laboratory capacity is essential for generating reliable and accurate data from clinical research, especially in<br />
resource-constrained settings. Local well-trained laboratory experts and scientists are important to research, and must<br />
participate actively in scientific activities and continuing education programs. Improving laboratory capacity is more than<br />
supplying new equipment and reagents; it also includes a long-term commitment to staff training, quality control, and<br />
biosafety. Improved laboratory capacity optimizes responses to an epidemic or an outbreak <strong>of</strong> a novel virulent pathogens,<br />
and can support international agendas to reduce the impact <strong>of</strong> pandemic influenza viruses. © <strong>2010</strong> Wertheim et al.<br />
No.1668<br />
Author (s) : West, T.E., Myers, N.D., Limmathurotsakul, D., Liggitt, H.D., Chantratita, N., Peacock, S.J., Skerrett, S.J.<br />
Title : Pathogenicity <strong>of</strong> high-dose enteral inoculation <strong>of</strong> Burkholderia pseudomallei to mice<br />
Source : American Journal <strong>of</strong> Tropical Medicine and Hygiene, Volume 83, Issue 5, November <strong>2010</strong>, Pages 1066-1069<br />
Document Type : Article<br />
Keywords : immunoglobulin G; animal experiment, animal model, animal tissue, antibody detection, article, bacterial infection,<br />
Burkholderia pseudomallei, chronic disease, clinical assessment, clinical feature, feces, feces analysis, female,<br />
immunoglobulin blood level, ingestion, inoculation, melioidosis, mouse, nonhuman, pathogenicity, saprotroph, adaptive<br />
immunity, animal, Bagg albino mouse, brain, C57BL mouse, enteritis, germfree animal, liver, lung, lymph node, mesentery,<br />
microbiology, pathology, spleen; Adaptive Immunity, Animals, Brain, Burkholderia pseudomallei, Enteritis, Feces, Female,<br />
Immunoglobulin G, Liver, Lung, Lymph Nodes, Melioidosis, Mesentery, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Specific<br />
Pathogen-Free Organisms, Spleen<br />
<strong>Abstract</strong> : Melioidosis is a frequently lethal tropical infection caused by the environmental saprophyte Burkholderia pseudomallei.<br />
Although transcutaneous inoculation and inhalation are considered the primary routes <strong>of</strong> infection, suggestive clinical<br />
evidence implicates ingestion as a possible alternative route. We show that in BALB/c and C57BL/6 mice, direct gastric<br />
inoculation <strong>of</strong> high doses <strong>of</strong> B. pseudomallei causes systemic infection that may be lethal or cause chronic disseminated<br />
infection. Mice may shed bacteria in the stool for weeks after infection, and high titers <strong>of</strong> B. pseudomallei-specific IgG are<br />
detectable. This report <strong>of</strong> enteric murine melioidosis supports further consideration <strong>of</strong> this route <strong>of</strong> infection. Copyright ©<br />
<strong>2010</strong> by The American Society <strong>of</strong> Tropical Medicine and Hygiene.<br />
No.1669<br />
Author (s) : Wetchateng, T., Friedman, C.S., Wight, N.A., Lee, P.-Y., Teng, P.H., Sriurairattana, S., Wongprasert, K., Withyachumnarnkul, B.<br />
Title : Withering syndrome in the abalone Haliotis diversicolor supertexta<br />
Source : Diseases <strong>of</strong> Aquatic Organisms, Volume 90, Issue 1, 18 May <strong>2010</strong>, Pages 69-76<br />
Document Type : Article<br />
Keywords : 'Candidatus Xenohaliotis californiensis'; 16S rDNA; Abalone; California; Haliotis diversicolor; People's Republic <strong>of</strong><br />
China; Taiwan; Thailand; Withering syndrome<br />
<strong>Abstract</strong> : Abalone aquaculture is a small but growing industry in Thailand and is based on both the exotic Haliotis diversicolor<br />
supertexta and the native H. asinina. Withering syndrome (WS) in abalone is caused by an infection with the Rickettsia-like<br />
organism (RLO) 'Candidatus Xenohaliotis californiensis' and has been spread to many countries globally. The present study<br />
reports the first observation <strong>of</strong> the WS-RLO agent in the small abalone, H. diversicolor supertexta in Thailand, Taiwan (ROC)<br />
and the People's Republic <strong>of</strong> China (PRC). Under light microscopy, the RLO was observed as intracytoplasmic inclusions within<br />
epithelial cells lining the post-esophagus and, to a minor extent, the intestine <strong>of</strong> H. diversicolor. Under transmission electron<br />
microscopy, inclusions were characterized as colonies <strong>of</strong> rod-shaped bacteria, 200 × 1800 nm in size, within a vesicle in the<br />
cytoplasm <strong>of</strong> the infected cell. The RLO from the small abalone bound with WS-RLO-specific in situ hybridization probes and<br />
was amplified by polymerase chain reaction (PCR), using primers designed from the 16S rDNA sequence <strong>of</strong> the original WS-<br />
RLO from California, USA. The PCR product <strong>of</strong> RLO samples from both the PRC and Thailand showed extremely high identity<br />
with the California WS-RLO (100 and 99%, respectively). These data combined with the history <strong>of</strong> abalone movements for<br />
aquaculture purposes indicate that RLOs observed in Thailand, Taiwan and the PRC are the WS-RLO that originated from<br />
California. © Inter-Research <strong>2010</strong>.<br />
609
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.1670<br />
Author (s) : Wetwatana, U., Kim-Lohsoontorn, P., Assabumrungrat, S., Laosiripojana, N.<br />
Title : Catalytic steam and autothermal reforming <strong>of</strong> used lubricating oil (ULO) over Rh- and Ni-based catalysts<br />
Source : Industrial and Engineering Chemistry Research, Volume 49, Issue 21, 3 November <strong>2010</strong>, Pages 10981-10985<br />
Document Type : Conference Paper<br />
Keywords : Autothermal reforming, Carbon deposition, Ni-based catalyst, Rh-based catalyst, Steam reforming, Steam-reforming conditions;<br />
Cerium, Cerium compounds, Hydrocarbons, Lubricating oils, Lubrication, Rhodium, Steam, Steam engineering, Zirconium<br />
alloys; Catalysts<br />
<strong>Abstract</strong> : The steam reforming <strong>of</strong> used lubricating oil (ULO) over Ni- and Rh-based catalysts supported by Ce-ZrO2 and Al2O3 is studied<br />
in the present work. Among all catalysts, Rh/Ce-ZrO2 (5 wt % Rh) provides relatively higher reforming reactivity (in terms <strong>of</strong><br />
ULO conversion and H2 yield) with excellent resistance toward carbon deposition compared to the other three catalysts. At<br />
850 °C after exposure in the steam reforming condition for 72 h, H2 yield <strong>of</strong> 78.1% can be achieved from the reaction over<br />
Rh/Ce-ZrO2. For Ni/Ce-ZrO2 and Ni/Al2O3, significant deactivations with time are detected and H2 yields <strong>of</strong> 55.3-58.7% are<br />
achieved after exposure for 72 h; in addition, considerable amounts <strong>of</strong> C2H4, C 2H6 and C3H6 are also found in the product<br />
along with H2, CO, CO2, and CH4. The effect <strong>of</strong> O2 addition was further studied over Ni/Ce-ZrO2 and Ni/Al2O3. It was found<br />
that this addition significantly reduces the degree <strong>of</strong> carbon deposition as well as promoting the conversion <strong>of</strong> hydrocarbons<br />
to CO and H2. At a suitable amount <strong>of</strong> O2, a high H2 yield comparable to that <strong>of</strong> steam reforming over Rh-based catalysts<br />
can be achieved. Nevertheless, it must be noted that introducing too high an O2 content resulted in lower H2 production<br />
due to the combustion <strong>of</strong> H2 and ULO by O2 addition. © <strong>2010</strong> American Chemical Society.<br />
No.1671<br />
Author (s) : Whangsuk, W., Dubbs, J.M., Sallabhan, R., Somsongkul, K., Mongkolsuk, S., Loprasert, S.<br />
Title : ChpR is a chlorpyrifos-responsive transcription regulator in Sinorhizobium meliloti<br />
Source : Journal <strong>of</strong> Molecular Microbiology and Biotechnology, Volume 18, Issue 3, June <strong>2010</strong>, Pages 141-147<br />
Document Type : Article<br />
Keywords : Biosensor; Insecticide responsive promoter; Pesticide; Transcription activator<br />
<strong>Abstract</strong> : The broad-spectrum organophosphate insecticide chlorpyrifos (CPF)-inducible locus, chpAB, was identified on the<br />
endogenous plasmid pSymB in Sinorhizobium meliloti. The S. meliloti chpA promoter was highly induced by CPF and was<br />
induced at much lower levels by diazinon and ethion. Transcription <strong>of</strong> chpA was dependent on chpR, a CadC family<br />
transcriptional regulator located upstream <strong>of</strong>, and divergently transcribed from, chpAB. ChpR was able to mediate the CPFinducible<br />
expression <strong>of</strong> the S. melilotichpA promoter in Escherichia coli through direct interaction with the chpAB promoter.<br />
The chpR-chpA intergenic regions <strong>of</strong> several bacterial chpRAB operons were aligned and a putative ChpR-binding sequence<br />
was proposed. Both the ChpR transcription factor and chpA promoter constitute a good candidate system for genetic-based<br />
biosensor development. © <strong>2010</strong> S. Karger AG, Basel.<br />
No.1672<br />
Author (s) : White, L.J., Schukken, Y.H., Dogan, B., Green, L., Döpfer, D., Chappell, M.J., Medley, G.F.<br />
Title : Modelling the dynamics <strong>of</strong> intramammary E. coli infections in dairy cows: understanding mechanisms that distinguish transient<br />
from persistent infections.<br />
Source : Veterinary research, Volume 41, Issue 2, January <strong>2010</strong><br />
Document Type : Article<br />
Keywords : Escherichia coli; Mastitis; Mathematical model; Persistent infection<br />
<strong>Abstract</strong> : The majority <strong>of</strong> intramammary infections with Escherichia coli in dairy cows result in transient infections with duration <strong>of</strong><br />
about 10 days or less, although more persistent infections (2 months or longer) have been identified. We apply a<br />
mathematical model to explore the role <strong>of</strong> an intracellular mammary epithelial cell reservoir in the dynamics <strong>of</strong> infection.<br />
We included biological knowledge <strong>of</strong> the bovine immune response and known characteristics <strong>of</strong> the bacterial population in<br />
both transient and persistent infections. The results indicate that varying the survival duration <strong>of</strong> the intracellular reservoir<br />
reproduces the data for both transient and persistent infections. Survival in an intracellular reservoir is the most likely<br />
mechanism that ensures persistence <strong>of</strong> E. coli infections in mammary glands. Knowledge <strong>of</strong> the pathogenesis <strong>of</strong> persistent<br />
infections is essential to develop preventive and treatment programmes for these important infections in dairy cows. © <strong>2010</strong><br />
INRA, EDP Sciences.<br />
610
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.1673<br />
Author (s) : White, N.J.<br />
Title : Artemisinin resistance - The clock is ticking<br />
Source : The Lancet, Volume 376, Issue 9758, 18 December <strong>2010</strong>, Pages 2051-2052<br />
Document Type : Note<br />
Keywords : artemisinin, chloroquine, pyrimethamine plus sulfadoxine; Cambodia, epidemiological data, human, international<br />
cooperation, malaria control, malaria falciparum, medical decision making, medical ethics, note, priority journal, world health<br />
organization; Africa, Anti-Infective Agents, Artemisinins, Asia, Southeastern, Cambodia, Drug Resistance, Humans, India, Malaria,<br />
Falciparum, Plasmodium falciparum, Travel, World Health<br />
<strong>Abstract</strong> : –<br />
No.1674<br />
Author (s) : White, N.J., Turner, G.D.H., Medana, I.M., Dondorp, A.M., Day, N.P.J.<br />
Title : The murine cerebral malaria phenomenon<br />
Source : Trends in Parasitology, Volume 26, Issue 1, January <strong>2010</strong>, Pages 11-15<br />
Document Type : Article<br />
Keywords : acetylcysteine, acetylsalicylic acid, activated carbon, albumin, arginine, celecoxib, deferoxamine, dexamethasone,<br />
erythropoietin, human albumin, indometacin, levamisole, mannitol, pentoxifylline, simvastatin, tumor necrosis factor<br />
antibody; article, brain malaria, clinical trial, disease model, drug effect, drug efficacy, drug response, enteric feeding,<br />
histopathology, human, inflammation, mouse, nonhuman, pathophysiology, Plasmodium berghei, Plasmodium berghei<br />
infection; Animals, Humans, Malaria, Cerebral, Mice, Mice, Inbred C57BL, Mice, Inbred CBA, Plasmodium berghei; Murinae, Mus<br />
<strong>Abstract</strong> : P.berghei ANKA infection in CBA or CB57BL/6 mice is used widely as a murine 'model' <strong>of</strong> human cerebral malaria (HCM),<br />
despite markedly different histopathological features. The pathology <strong>of</strong> the murine model is characterised by marked<br />
inflammation with little or no intracerebral sequestration <strong>of</strong> parasitised erythrocytes, whereas HCM is associated with intense<br />
intracerebral sequestration, <strong>of</strong>ten with little inflammatory response. There are now more than ten times as many studies<br />
each year <strong>of</strong> the murine model than on HCM. Of 48 adjunctive interventions evaluated in the murine model, 44 (92%) were<br />
successful, compared with only 1 (6%) <strong>of</strong> 17 evaluated in HCM during the same period. The value <strong>of</strong> the mouse model in<br />
identifying pathological processes or therapeutic interventions in human cerebral malaria is questionable. © 2009 Elsevier Ltd.<br />
All rights reserved.<br />
No.1675<br />
Author (s) : Wiboonchutikul, S., Sungkanuparph, S., Sukasem, C., Wathipthun, C., Chantratita, W.<br />
Title : Comparison <strong>of</strong> protease inhibitor (PI) resistance-associated mutations between PI-naïve and PI-experienced HIV-1 infected<br />
patients in Thailand where subtype A/E is predominant<br />
Source : Current HIV Research, Volume 8, Issue 6, <strong>2010</strong>, Pages 456-460<br />
Document Type : Article<br />
Keywords : CRF 01_AE; HIV; Mutations; Protease inhibitor; Resistance; Subtype A/E<br />
<strong>Abstract</strong> : Protease inhibitor (PI) resistance-associated mutations (RAMs) are commonly observed in PI-naïve patients who are infected<br />
with HIV-1 subtype A/E. Few data are available on the genetic mechanisms <strong>of</strong> PI resistance in non-B HIV-1. This study was<br />
aimed to compare PI-RAMs between PI-naïve and -experienced patients and determine PI resistance in each group. Genotypic<br />
resistance testing was conducted among a cohort <strong>of</strong> HIV-1-infected patients who were diagnosed with virologic failure. We<br />
studied 137 patients <strong>of</strong> whom 75 patients were in PI-naïve group and 62 patients in PI-experienced group. Median CD4 cell<br />
count and HIV-1 RNA at virologic failure were 169 cells/mm3 and 14,100 copies/mL, respectively. The clinical characteristics<br />
between 2 groups were similar (p>0.05) except for the duration <strong>of</strong> antiretroviral therapy (ART) which was shorter in PI-naïve<br />
group (31.5 vs 46.8 months, p=0.028). Proportion <strong>of</strong> patients with primary PI-RAMs was 2.7% in PI-naïve and 19% in PIexperienced<br />
groups (p=0.002). The most common primary PI-RAMs in the latter group were V82A (10%), I54V (7%) and G48V<br />
(4.8%). Proportion <strong>of</strong> patients with secondary PI-RAMs in the corresponding groups was 99% and 98%, respectively (p=1.000).<br />
The most common secondary PI-RAMs in both groups were M36I (91%), H69K (34%) and L89M (30%). In conclusion, primary<br />
PI-RAMs are observed exclusively among PI-experienced patients, whereas secondary PI-RAMs are equally found in both PInaïve<br />
and PI-experienced patients. Further studies to define virologic response <strong>of</strong> HIV-1 subtype A/E to various PIs and clinical<br />
validation <strong>of</strong> PI-RAMs in HIV-1 subtype A/E are essentially needed. © <strong>2010</strong> Bentham Science Publishers Ltd.<br />
611
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.1676<br />
Author (s) : Wiersinga, W.J., Calandra, T., Kager, L.M., Van Der Windt, G.J.W., Roger, T., Le Roy, D., Florquin, S., Peacock, S.J., Sweep, F.C.G.J.,<br />
Van Der Poll, T.<br />
Title : Expression and function <strong>of</strong> macrophage Migration Inhibitory Factor (MIF) in melioidosis<br />
Source : PLoS Neglected Tropical Diseases, Volume 4, Issue 2, February <strong>2010</strong>, Article number e605<br />
Document Type : Article<br />
Keywords : macrophage migration inhibition factor, recombinant protein, isomerase, Mif protein, mouse; animal experiment, animal<br />
model, article, Burkholderia pseudomallei, clinical article, controlled<br />
study, human, leukocyte, male, melioidosis, mortality, mouse, nonhuman, pneumonia, protein blood level, protein<br />
expression, protein function, animal, bacterial count, biosynthesis, C57BL<br />
mouse, chemistry, immunology, lung, microbiology, plasma, Southeast Asia; Animals, Asia, Southeastern, Burkholderia<br />
pseudomallei, Colony Count, Microbial, Humans, Intramolecular Oxidoreductases, Leukocytes, Lung, Macrophage Migration-<br />
Inhibitory Factors, Male, Melioidosis, Mice, Mice, Inbred C57BL, Plasma<br />
<strong>Abstract</strong> : Background: Macrophage migration inhibitory factor (MIF) has emerged as a pivotal mediator <strong>of</strong> innate immunity and has been<br />
shown to be an important effector molecule in severe sepsis. Melioidosis, caused by Burkholderia pseudomallei, is an<br />
important cause <strong>of</strong> community-acquired sepsis in Southeast-Asia. We aimed to characterize the expression and function <strong>of</strong><br />
MIF in melioidosis. Methodology and Principal Findings: MIF expression was determined in leukocytes and plasma from 34<br />
melioidosis patients and 32 controls, and in mice infected with B. pseudomallei. MIF function was investigated in<br />
experimental murine melioidosis using anti-MIF antibodies and recombinant MIF. Patients demonstrated markedly increased<br />
MIF mRNA leukocyte and MIF plasma concentrations. Elevated MIF concentrations were associated with mortality. Mice<br />
inoculated intranasally with B. pseudomallei displayed a robust increase in pulmonary and systemic MIF expression. Anti-MIF<br />
treated mice showed lower bacterial loads in their lungs upon infection with a low inoculum. Conversely, mice treated with<br />
recombinant MIF displayed a modestly impaired clearance <strong>of</strong> B. pseudomallei. MIF exerted no direct effects on bacterial<br />
outgrowth or phagocytosis <strong>of</strong> B. pseudomallei. Conclusions: MIF concentrations are markedly elevated during clinical<br />
melioidosis and correlate with patients' outcomes. In experimental melioidosis MIF impaired antibacterial defense. © <strong>2010</strong><br />
Wiersinga et al.<br />
No.1677<br />
Author (s) : Wiersinga, W.J., Kager, L.M., Hovius, J.W.R., Van Der Windt, G.J.W., De Vos, A.F., Meijers, J.C.M., Roel<strong>of</strong>s, J.J., Dondorp, A., Levi,<br />
M., Day, N.P., Peacock, S.J., Van Der Poll, T.<br />
Title : Urokinase receptor is necessary for bacterial defense against pneumonia-derived septic melioidosis by facilitating phagocytosis<br />
Source : Journal <strong>of</strong> Immunology, Volume 184, Issue 6, 15 March <strong>2010</strong>, Pages 3079-3086<br />
Document Type : Article<br />
Keywords : messenger RNA, urokinase receptor; adult, aged, animal cell, animal model, antigen expression, antigen<br />
function, article, bacterial growth, bacterial immunity, Burkholderia pseudomallei, cell migration, clinical article, controlled<br />
study, granulocyte, hepatitis, human, human cell, in vitro<br />
study, melioidosis, monocyte, mouse, neutrophil, nonhuman, phagocytosis, pneumonia, priority journal, wild<br />
type, adolescent, animal, bacteremia, bacterial pneumonia, biosynthesis, C57BL mouse, cell culture, disease<br />
model, genetics, immunology, microbiology, middle aged, migration inhibition, mouse mutant, neutrophil<br />
chemotaxis, pathology, physiology; Adolescent, Adult, Aged, Aged, 80 and over, Animals, Bacteremia, Burkholderia<br />
pseudomallei, Cell Migration Inhibition, Cells, Cultured, Disease Models, Animal, Humans, Melioidosis, Mice, Mice, Inbred<br />
C57BL, Mice, Knockout, Middle Aged, Neutrophil Infiltration, Neutrophils, Phagocytosis, Pneumonia, Bacterial, Receptors,<br />
Urokinase Plasminogen Activator<br />
<strong>Abstract</strong> : Urokinase receptor (urokinase-type plasminogen activator receptor [uPAR], CD87), a GPI-anchored protein, is considered to<br />
play an important role in inflammation and fibrinolysis. The Gram-negative bacterium Burkholderia pseudomallei is able to<br />
survive and replicate within leukocytes and causes melioidosis, an important cause <strong>of</strong> pneumonia-derived communityacquired<br />
sepsis in Southeast Asia. In this study, we investigated the expression and function <strong>of</strong> uPAR both in patients with<br />
septic melioidosis and in a murine model <strong>of</strong> experimental melioidosis. uPAR mRNA and surface expression was increased in<br />
patients with septic melioidosis in/on both peripheral blood monocytes and granulocytes as well as in the pulmonary<br />
compartment during experimental pneumonia-derived melioidosis in mice. uPAR-deficient mice intranasally infected with B.<br />
pseudomallei showed an enhanced growth and dissemination <strong>of</strong> B. pseudomallei when compared with wild-type mice,<br />
corresponding with increased pulmonary and hepatic inflammation. uPAR knockout mice demonstrated significantly reduced<br />
612
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
neutrophil migration toward the pulmonary compartment after inoculation with B. pseudomallei. Further in vitro experiments<br />
showed that uPAR-deficient macrophages and granulocytes display a markedly impaired phagocytosis <strong>of</strong> B. pseudomallei.<br />
Additional studies showed that uPAR deficiency did not influence hemostatic and fibrinolytic responses during severe<br />
melioidosis. These data suggest that uPAR is crucially involved in the host defense against sepsis caused by B. pseudomallei<br />
by facilitating the migration <strong>of</strong> neutrophils toward the primary site <strong>of</strong> infection and subsequently facilitating the phagocytosis<br />
<strong>of</strong> B. pseudomallei. Copyright © <strong>2010</strong> by The American Association <strong>of</strong> Immunologists, Inc.<br />
No.1678<br />
Author (s) : Wilairatana, P., Krudsood, S., Tangpukdee, N.<br />
Title : Appropriate time for primaquine treatment to reduce Plasmodium falciparum transmission in hypoendemic areas<br />
Source : Korean Journal <strong>of</strong> Parasitology, Volume 48, Issue 2, June <strong>2010</strong>, Pages 179-182<br />
Document Type : Article<br />
Keywords : Artemisinin-combination therapy; Malaria transmission blocking; Plasmodium falciparum; Primaquine<br />
<strong>Abstract</strong> : Artemesinin-combination therapies (ACT) for falciparum malaria reduce gametocyte carriage, and therefore reduce<br />
transmission. Artemisinin derivatives will act against only young gametocytes whereas primaquine acts on mature<br />
gametocytes which are present usually in the circulation at the time when the patient presents for treatment. Both<br />
artemisinin derivatives and primaquine have short half-lives, less than 1 hr and 7 hr, respectively. Therefore, asexual parasites<br />
or young gametocytes remain after completed ACT. A single dose <strong>of</strong> primaquine (0.50-0.75 mg base/kg) at the end <strong>of</strong> ACT can<br />
kill only mature gametocytes but cannot kill young gametocytes (if present). Remaining asexual forms after completion <strong>of</strong><br />
ACT course, e.g., artesunate-mefloquine for 3 days, may develop to mature gametocytes 7-15 days later. Thus, an additional<br />
dose <strong>of</strong> primaquine (0.50-0.75 mg base/kg) given 2 weeks after ACT completion may be beneficial for killing remaining mature<br />
gametocytes and contribute to more interruption <strong>of</strong> Plasmodium falciparum transmission than giving only 1 single dose <strong>of</strong><br />
primaquine just after completing ACT.<br />
No.1679<br />
Author (s) : Wilairatana, P., Krudsood, S., Tangpukdee, N.<br />
Title : Management <strong>of</strong> Plasmodium knowlesi malaria without PCR confirmation<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 1, January <strong>2010</strong>, Pages 19-21<br />
Document Type : Article<br />
Keywords : Management; PCR; Plasmodium knoivlesi<br />
<strong>Abstract</strong> : Plasmodium knoivlesi morphologically resembles P. malariae; PCR assays are able to differentiate between the 2 species<br />
correctly. However, PCR is not available in many hospitals in P. knoivlesi endemic areas, particularly in Southeast Asia. In<br />
places where PCR is not available, anti-malarial drugs for P. malariae or other non-P. falciparum or P. falciparum species are<br />
effective against P. knoivlesi. Even with a wrong diagnosis <strong>of</strong> another malaria species by light microscopy instead <strong>of</strong> P.<br />
knoivlesi, the antimalarial drugs given are still effective for treating P. knoivlesi infection.<br />
No.1680<br />
Author (s) : Wilairatana, P., Krudsood, S., Tangpukdee, N.<br />
Title : Vitamin K injection in spontaneous bleeding and coagulopathy in severe malaria: Pros and cons<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 3, May <strong>2010</strong>, Pages 514-516<br />
Document Type : Article<br />
Keywords : Coagulopathy; Severe malaria; Spontaneous bleeding; Vitamin K<br />
<strong>Abstract</strong> : Not all clinicians give vitamin K to severe malaria patients with systemic bleeding. Vitamin K injections may not be useful to<br />
stop bleeding in severe malaria patients with predominant hepatocellular jaundice. However, vitamin K may be justified in<br />
bleeding patients who have prolonged fasting <strong>of</strong> more than 3-7 days, underlying malnutrition, or predominant cholestatic<br />
jaundice. The decision to give vitamin K to severe malaria patients with systemic bleeding should be based on underlying<br />
diseases, type <strong>of</strong> jaundice, risk for vitamin K deficiency, and allergy to the drug.<br />
No.1681<br />
Author (s) : Wilairatana, P., Tangpukdee, N., Jittaseree, L.<br />
Title : Cruveilhier-baumgarten syndrome in a cirrhotic patient taking alcohol with boesenbergia pundurata<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 5, September <strong>2010</strong>, Pages 1267-1270<br />
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Document Type : Article<br />
Keywords : Alcohol; Boesenbergia pundurata; Cirrhosis; Cruveilhier-baumgarten syndrome<br />
<strong>Abstract</strong> : Cruveilhier-Baumgarten syndrome (CBS) is a rare complication <strong>of</strong> cirrhosis. We reported a case <strong>of</strong> CBS who came to hospital<br />
with progressive ascites, jaundice, fever and upper gastrointestinal bleeding. The cause <strong>of</strong> cirrhosis and hepatitis might be due<br />
to alcohol and possibly local Thai herb [Boesenbergia pundurata (Roxb.) Schitr or Krachaidum].<br />
No.1682<br />
Author (s) : Wilairatana, P., Tangpukdee, N., Kano, S., Krudsood, S.<br />
Title : Primaquine administration after falciparum malaria treatment in malaria hypoendemic areas with high incidence <strong>of</strong> falciparum<br />
and vivax mixed infection: Pros and cons<br />
Source : Korean Journal <strong>of</strong> Parasitology, Volume 48, Issue 2, June <strong>2010</strong>, Pages 175-177<br />
Document Type : Article<br />
Keywords : Mixed infection; Plasmodium falciparum; Plasmodium vivax; Primaquine<br />
<strong>Abstract</strong> : Mixed infections <strong>of</strong> Plasmodium falciparum and Plasmodium vivax is high (~30%) in some malaria hypoendemic areas where<br />
the patients present with P. falciparum malaria diagnosed by microscopy. Conventional treatment <strong>of</strong> P. falciparum with<br />
concurrent chloroquine and 14 days <strong>of</strong> primaquine for all falciparum malaria patients may be useful in areas where mixed<br />
falciparum and vivax infections are high and common and also with mild or moderate G6PD deficiency in the population<br />
even with or without subpatent vivax mixed infection. It will be possibly cost-effective to reduce subsequent vivax illness if<br />
the patients have mixed vivax infection. Further study to prove this hypothesis may be warranted.<br />
No.1683<br />
Author (s) : Wilairatana, P., Tangpukdee, N., Krudsood, S.<br />
Title : Long term primaquine administration to reduce plasmodium falciparum gametocyte transmission in hypoendemic areas<br />
Source : Southeast Asian Journal <strong>of</strong> Tropical Medicine and Public Health, Volume 41, Issue 6, November <strong>2010</strong>, Pages 1306-1311<br />
Document Type : Article<br />
Keywords : Artemesinin-combination therapies; Malaria transmission blocking; Plasmodium falciparum; Primaquine<br />
<strong>Abstract</strong> : Artemesinin-combination therapies (ACTs) for falciparum malaria reduce gametocyte carriage, and therefore reduce<br />
transmission. Artemisinin derivatives act only against young gametocytes, but primaquine acts against mature gametocytes<br />
(which are usually present in the circulation at the time the patient presents for treatment). Both artemisnin derivatives and<br />
primaquine have short half-lives (less than 1 hour and 8 hours, respectively). Therefore, asexual parasites and young<br />
gametocytes may remain after completing ACT. Single dose <strong>of</strong> primaquine (0.5-0.75 mg base/kg) at the end <strong>of</strong> ACT can kill<br />
only mature gametocytes (if present) but cannot kill young gametocytes (if present). Remaining asexual forms and<br />
sequestered young gametocytes remaining after completion <strong>of</strong> ACT may develop into mature gametocytes 7-15 days later.<br />
Some patients have the first appearance <strong>of</strong> gametocytemia 4-8/day after completion <strong>of</strong> ACT. Thus, additional doses <strong>of</strong><br />
primaquine (0.5-0.75 mg base/kg) given 15-18 days after or concurrently with 3 day-ACT respectively or given 15-22 days after<br />
or concurrently with 7 day- ACT respectively may be beneficial in killing the remaining mature gametocytes and thus<br />
contribute to interruption <strong>of</strong> P. falciparum gametocyte transmission more affectively than giving only a single dose <strong>of</strong><br />
primaquine just after completing ACT.<br />
No.1684<br />
Author (s) : Wilasrusmee, C., Suthakorn, J., Guerineau, C., Itsarachaiyot, Y., Sa-Ing, V., Proprom, N., Lertsithichai, P., Jirasisrithum, S., Kittur, D.<br />
Title : A novel robotic mon<strong>of</strong>ilament test for diabetic neuropathy<br />
Source : Asian Journal <strong>of</strong> Surgery, Volume 33, Issue 4, October <strong>2010</strong>, Pages 193-198<br />
Document Type : Article<br />
Keywords : diabetic foot; Semmes-Weinstein mon<strong>of</strong>ilament; Toronto Clinical Scoring System; vibration perception test<br />
<strong>Abstract</strong> : OBJECTIVE: The use <strong>of</strong> the Semmes-Weinstein (SW) mon<strong>of</strong>ilament test is recommended as a screening method for diabetic<br />
neuropathy. It <strong>of</strong>fers an important chance to prevent further complications <strong>of</strong> diabetic foot. We aimed to develop a<br />
prototype Robotic Mon<strong>of</strong>ilament Inspector that can be used as a standard machine for screening <strong>of</strong> diabetic neuropathy.<br />
METHODS: Development was divided into three parts: computer s<strong>of</strong>tware, control box, and Robotic Mon<strong>of</strong>ilament Inspector.<br />
The examiner conducted the SW test (by hand and by robotic inspector), vibration perception threshold, and Toronto Clinical<br />
Scoring System without knowledge <strong>of</strong> patient information. The unpaired t test or Wilcoxon rank-sum test was used to<br />
determine the differences between independent groups in terms <strong>of</strong> continuous outcomes, while the χ2 test was used to<br />
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determine categorical outcomes. Agreement between the various diabetic neuropathy tests was measured using the kappa<br />
statistic. RESULTS: The SW test and vibration perception threshold were more valid tests for neuropathy than the Toronto<br />
test. The robotic test was in excellent agreement with the two former tests and appeared to be valid (kappa statistic, 0.35-<br />
0.81). Another indirect evidence for the validity <strong>of</strong> the robotic test was the finding that diabetic patients with foot ulcers had a<br />
higher prevalence <strong>of</strong> neuropathy (77% vs. 38%). This might indicate that the robotic test was more valid than the manual<br />
test. CONCLUSION: The Robotic Mon<strong>of</strong>ilament Inspector could be used as a simple screening machine. This prototype may<br />
be developed further for routine clinical use. © <strong>2010</strong> Asian Surgical Association.<br />
No.1685<br />
Author (s) : Willemsen, I., Cooper, B., Van Buitenen, C., Winters, M., Andriesse, G., Kluytmans, J.<br />
Title : Improving quinolone use in hospitals by using a bundle <strong>of</strong> interventions in an interrupted time series analysis<br />
Source : Antimicrobial Agents and Chemotherapy, Volume 54, Issue 9, September <strong>2010</strong>, Pages 3763-3769<br />
Document Type : Article<br />
Keywords : ceftazidime, cefuroxime, cipr<strong>of</strong>loxacin, cotrimoxazole, tobramycin; antibiotic sensitivity, article, bacterium isolate, cost<br />
control, drug cost, drug monitoring, education program, Escherichia coli, hospital infection, hospital patient, human, major<br />
clinical study, outcome assessment, Poisson distribution, priority journal, regression analysis, time series analysis; Anti-Bacterial<br />
Agents, Cipr<strong>of</strong>loxacin, Hospitals, Humans, Injections, Intravenous, Prospective Studies, Quinolones<br />
<strong>Abstract</strong> : The objectives <strong>of</strong> the present study were to determine the effects <strong>of</strong> multiple targeted interventions on the level <strong>of</strong> use <strong>of</strong><br />
quinolones and the observed rates <strong>of</strong> resistance to quinolones in Escherichia coli isolates from hospitalized patients. A<br />
bundle consisting <strong>of</strong> four interventions to improve the use <strong>of</strong> quinolones was implemented. The outcome was measured<br />
from the monthly levels <strong>of</strong> use <strong>of</strong> intravenous (i.v.) and oral quinolones and the susceptibility patterns for E. coli isolates<br />
from hospitalized patients. Statistical analyses were performed using segmented regression analysis and segmented Poisson<br />
regression models. Before the bundle was implemented, the annual use <strong>of</strong> quinolones was 2.7 defined daily doses<br />
(DDDs)/100 patient days. After the interventions, in 2007, this was reduced to 1.7 DDDs/100 patient days. The first<br />
intervention, a switch from i.v. to oral medication, was associated with a stepwise reduction in i.v. quinolone use <strong>of</strong> 71<br />
prescribed daily doses (PDDs) per month (95% confidence interval [CI] = 47 to 95 PDDs/month, P < 0.001). Intervention 2,<br />
introduction <strong>of</strong> a new antibiotic guideline and education program, was associated with a stepwise reduction in the overall use<br />
<strong>of</strong> quinolones (reduction, 107 PDDs/month [95% CI = 58 to 156 PDDs/month). Before the interventions the quinolone<br />
resistance rate was increasing, on average, by 4.6% (95% CI = 2.6 to 6.1%) per year. This increase leveled <strong>of</strong>f, which was<br />
associated with intervention 2 and intervention 4, active monitoring <strong>of</strong> prescriptions and feedback. Trends in resistance to<br />
other antimicrobial agents did not change. This study showed that the hospital-wide use <strong>of</strong> quinolones can be significantly<br />
reduced by an active policy consisting <strong>of</strong> multiple interventions. There was also a stepwise reduction in the rate <strong>of</strong> quinolone<br />
resistance associated with the bundle <strong>of</strong> interventions. Copyright © <strong>2010</strong>, American Society for Microbiology. All Rights<br />
Reserved.<br />
No.1686<br />
Author (s) : Wiratkapun, C., Bunyapaiboonsri, W., Wibulpolprasert, B., Lertsithichai, P.<br />
Title : Biopsy rate and positive predictive value for breast cancer in BI-RADS category 4 breast lesions<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 7, July <strong>2010</strong>, Pages 830-837<br />
Document Type : Article<br />
Keywords : BI-RADS 4; Breast cancer; Core needle biopsy; Mammography<br />
<strong>Abstract</strong> : Objective: To examine the biopsy rate and positive predictive value (PPV) for breast cancer in lesions categorized as BI-RADS<br />
(Breast Imaging reporting and Data System) category 4. Material and Method: The medical records <strong>of</strong> patients with BIRADS 4<br />
breast lesion diagnosed at the breast diagnostic center, Ramathibodi Hospital between December 1, 2004 and December 31,<br />
2005 were retrospectively reviewed. PPV was calculated for patients who had biopsy performed or had clinical follow-up for<br />
at least two years. The radiographic and pathological findings were compared. Results: The biopsy rate <strong>of</strong> 536 lesions<br />
categorized as BI-RADS 4 was 75% (403 <strong>of</strong> 536). Malignancy was found in 95 <strong>of</strong> 460 patients; or a PPV <strong>of</strong> 21%, PPVs for<br />
subcategories 4A, 4B and 4C, were 9%, 21% and 57%, respectively. The most common malignancy was invasive ductal<br />
carcinoma (67%). Patients with advanced age, having a clinically palpable breast mass <strong>of</strong> large size, with mammographic<br />
findings <strong>of</strong> architectural distortion and asymmetrical density were significantly associated with a higher risk <strong>of</strong> breast cancer.<br />
Conclusion: PPV for malignancy in the present study was comparable to previous studies. In subcategories 4B and 4C, the<br />
malignancy rate was higher than that in subcategory 4A.<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.1687<br />
Author (s) : Wiratkapun, C., Fusuwankaya, E., Wibulpholprasert, B., Lertsittichai, P.<br />
Title : Diagnostic accuracy <strong>of</strong> vacuum-assisted stereotactic core needle biopsy for breast lesions<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 9, September <strong>2010</strong>, Pages 1058-1064<br />
Document Type : Article<br />
Keywords : Breast cancer; Breast lesions; Diagnostic accuracy; Stereotactic core needle biopsy; Underestimation rate<br />
<strong>Abstract</strong> : Objective: To evaluate the diagnostic accuracy <strong>of</strong> the vacuum-assisted stereotactic core needle biopsy (CNB) for breast<br />
lesions. Material and Method: Sixty-four lesions that had undergone vacuum-assisted stereotactic CNB between January 2003<br />
and December 2005 in Ramathibodi Breast diagnostic center were included in this study. Pathologic results <strong>of</strong> CNB were<br />
reviewed and correlated with pathologic results <strong>of</strong> subsequent open surgery. For benign lesions without surgery, the authors<br />
correlated the result <strong>of</strong> CNB with stability <strong>of</strong> the lesion at or more than 2-year interval follow-up. Agreement rate, high-risk<br />
under estimate rate, Ductal carcinoma in situ (DCIS) underestimate rate, false negative rate, and sensitivity were accessed.<br />
Results: The pathologic results for the CNB were malignancy in 20%, high-risk in 13%, and benign in 67%. The agreement rate<br />
was 93.8% (60 <strong>of</strong> 64). The under estimate rate for atypical ductal hyperplasia (ADH) was 50% (3 <strong>of</strong> 6). There was no<br />
underestimate for DCIS in the present study. Of 43 benign lesions, malignancy was found at subsequent open surgery in one<br />
lesion and false negative rate was 6%. Calculated sensitivity was 96%. Conclusion: Vacuum-assisted CNB is an accurate<br />
method for evaluating breast lesions. This procedure is an alternative to surgical excision for lesion assessments.<br />
No.1688<br />
Author (s) : Wiriyarat, W., Lerdsamran, H., Pooruk, P., Webster, R.G., Louisirirotchanakul, S., Ratanakorn, P., Chaichoune, K., Nateerom, K.,<br />
Puthavathana, P.<br />
Title : Erythrocyte binding preference <strong>of</strong> 16 subtypes <strong>of</strong> low pathogenic avian influenza and 2009 pandemic influenza A (H1N1)<br />
viruses<br />
Source : Veterinary Microbiology, Volume 146, Issue 3-4, 15 December <strong>2010</strong>, Pages 346-349<br />
Document Type : Article<br />
Keywords : 2009 pandemic influenza A (H1N1) virus; Erythrocytes; Hemagglutination assay; Low pathogenic avian influenza viruses<br />
<strong>Abstract</strong> : All 16 subtypes <strong>of</strong> avian influenza viruses <strong>of</strong> low pathogenicity (LPAIV) as well as their hemagglutinin (H) antigens, and four<br />
2009 pandemic influenza A (H1N1) virus isolates were assayed for hemagglutinating activity against 5 erythrocyte species:<br />
goose, guinea pig, human group O, chicken and horse. Of all viruses and antigens assayed, the highest hemagglutination (HA)<br />
titers were obtained with goose and guinea pig erythrocytes. Hemagglutinating activity <strong>of</strong> replicating LPAIV and LPAIV antigens<br />
decreased, in order, with chicken and human group O; meanwhile, horse erythrocytes yielded lowest or no HA titer.<br />
Moreover, the 2009 pandemic viruses did not agglutinate both horse and chicken erythrocytes. Our study concluded that<br />
goose and guinea pig erythrocytes are the best in HA assay for all subtypes <strong>of</strong> influenza viruses. © <strong>2010</strong> Elsevier B.V.<br />
No.1689<br />
Author (s) : Wisedsukol, P., Chanyagom, P., Subphachaisirikul, K.<br />
Title : Heterogeneous information integration and management models in Thai justice information systems<br />
Source : Proceedings - <strong>2010</strong> 3rd IEEE <strong>International</strong> Conference on Computer Science and Information Technology, ICCSIT <strong>2010</strong>, Volume<br />
4, <strong>2010</strong>, Article number 5564942, Pages 269-273<br />
Document Type : Conference Paper<br />
Keywords : Information integration; Information management; Middleware; REST; ROA<br />
<strong>Abstract</strong> : The Thai justice has been using Information systems in their routine works for nearly 20 years. Around 1993, Thailand<br />
attempted to integrate information systems (IS) <strong>of</strong> all agencies in justice system. Unfortunately, the result was unsuccessful<br />
and the project was terminated. The critical but common problems are data duplication and data inconsistency. Because<br />
most <strong>of</strong> Thai government agencies have been using their own information systems designed based on the agencies'<br />
requirements, which normally developed by outsourcers or contractors. As a result, any attempt <strong>of</strong> information integration<br />
cause data duplication and data inconsistency problems. However, these problems can be theoretically solved by using<br />
global unique identifier such as citizen identifier number or common attributes that are available in local sources. In practical,<br />
only using global unique identifier may not be sufficient. Mapping model and data cleansing are still in need. This work<br />
presents suitable integration information model, aiming to resolve these problems. However the non-technical problems such<br />
as legal issue and right protection cannot be achieved by using only data integration model. The suitable information<br />
management model is, therefore, critical and described in this work. By combining these two models, the research shows that<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
it is able to overcome cultural organizations and legal issue as well as all technical problems. The techniques and<br />
methodologies including data integration concept, resource oriented architecture (ROA), and the management model<br />
proposed here help to promote transparency, accountability and auditability <strong>of</strong> all information systems in the Thai justice. ©<br />
<strong>2010</strong> IEEE.<br />
No.1690<br />
Author (s) : Wisessombat, S., Kittiniyom, K., Srimanote, P., Wonglumsom, W., Voravuthikunchai, S.P.<br />
Title : Enhancement <strong>of</strong> viable Campylobacter detection by chemotactic stimuli<br />
Source : Journal <strong>of</strong> Microbiological Methods, Volume 82, Issue 2, August <strong>2010</strong>, Pages 170-176<br />
Document Type : Article<br />
Keywords : Chemotaxis; Chicken; Motility ability; Thermophilic Campylobacter<br />
<strong>Abstract</strong> : The effects <strong>of</strong> chemotactic stimuli on motility ability <strong>of</strong> viable Campylobacter to pass through a 0.45μm pore size filter in<br />
viscous condition were investigated. Reference strains including C. jejuni ATCC 33291, C. coli MUMT 18407, C. lari ATCC 43675,<br />
and C. upsaliensis DMST 19055 were used. The initial numbers <strong>of</strong> artificially-inoculated viable cells per g <strong>of</strong> chicken meat<br />
were approximately 10 to 104. Constituents <strong>of</strong> mucin plus bile (1:1), varieties <strong>of</strong> amino acids, and sodium salts were added<br />
into a s<strong>of</strong>t-agar-coated membrane filter and incubated at both 37°C and 42°C for 24h. The drop plate method was used to<br />
determine numbers <strong>of</strong> viable Campylobacter at 6, 12, 18, and 24h. After 6h, constituents <strong>of</strong> mucin plus bile at the<br />
concentrations <strong>of</strong> 1, 5, and 10% demonstrated significant increases in numbers <strong>of</strong> viable cells (p<0.05). The numbers <strong>of</strong> the<br />
organisms at 42°C were higher than those at 37°C. In contrast, no significant difference in cell numbers was observed by<br />
adding amino acids or sodium salts. In addition, the role <strong>of</strong> starvation on chemotactic responses was also studied. Starved<br />
cells showed lower chemotactic response than non-starved cells. This method permitted rapid detection <strong>of</strong> viable<br />
thermophilic Campylobacter. © <strong>2010</strong> Elsevier B.V.<br />
No.1691<br />
Author (s) : Witoonpanich, R., Vichayanrat, E., Tantisiriwit, K., Wongtanate, M., Sucharitchan, N., Oranrigsupak, P., Chuesuwan, A., Nakarawat,<br />
W., Tima, A., Suwatcharangkoon, S., Ingsathit, A., Rattanasiri, S., Wananukul, W.<br />
Title : Survival analysis for respiratory failure in patients with food-borne botulism<br />
Source : Clinical Toxicology, Volume 48, Issue 3, March <strong>2010</strong>, Pages 177-183<br />
Document Type : Article<br />
Keywords : Botulinum antitoxin; Difficulty swallowing; Endotracheal intubation; Ophthalmoplegia; Proximal muscle weakness; Ptosis<br />
<strong>Abstract</strong> : Introduction. Botulism is a rare presynaptic neuromuscular junction disorder caused by potent toxins produced by the<br />
anaerobic, spore-forming, Gram-positive bacterium Clostridium botulinum. Food-borne botulism is caused by the ingestion <strong>of</strong><br />
foods contaminated with botulinum toxin. In March 2006, there was a large outbreak <strong>of</strong> food-borne botulism associated with<br />
the ingestion <strong>of</strong> home-canned bamboo shoots in Thailand. The survival analyses for respiratory failure in these patients were<br />
studied and are reported here. Methods. A prospective observational cohort study was conducted on this outbreak. The<br />
primary outcome <strong>of</strong> interest was the time to respiratory failure. The secondary outcome was the time to weaning <strong>of</strong>f<br />
ventilator. The prognostic factors associated with respiratory failure and weaning <strong>of</strong>f ventilator are presented. Results. A total<br />
<strong>of</strong> 91 in-patients with baseline clinical characteristics were included. Most cases first presented with gastrointestinal symptoms<br />
followed by neurological symptoms, the most striking <strong>of</strong> which being difficulty in swallowing. Common clinical features<br />
included ptosis, ophthalmoplegia, proximal muscle weakness, pupillary abnormality, and respiratory failure. Forty-two<br />
patients developed respiratory failure requiring mechanical ventilation and the median duration on ventilator was 14 days.<br />
The median length <strong>of</strong> hospital stay for all patients was 13.5 days. Difficulty in breathing, moderate to severe ptosis, and<br />
dilated and fixed pupils were associated with respiratory failure. Among patients who were on ventilators, a short incubation<br />
period and pupillary abnormality were associated with a longer period <strong>of</strong> mechanical ventilation. All patients had antitoxin<br />
injection and there was no mortality in this outbreak. Conclusion. The history <strong>of</strong> difficult breathing and the findings <strong>of</strong><br />
moderate to severe ptosis and pupillary abnormality were associated with severe illness and respiratory failure. A long<br />
incubation time was associated with a better prognosis. Although botulism is a potentially fatal disease, there was no<br />
mortality in this outbreak. All patients had antitoxin injection and good intensive care that resulted in good clinical outcomes.<br />
Copyright © Informa UK, Ltd.<br />
No.1692<br />
Author (s) : Witoonsaridsilp, W., Panyarachun, B., Sarisuta, N., Müller-Goymann, C.C.<br />
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Title : Influence <strong>of</strong> microenvironment and liposomal formulation on secondary structure and bilayer interaction <strong>of</strong> lysozyme<br />
Source : Colloids and Surfaces B: Biointerfaces, Volume 75, Issue 2, 1 February <strong>2010</strong>, Pages 501-509<br />
Document Type : Article<br />
Keywords : Charged liposomes; Conformation; Lysozyme; Protein-lipid binding; Secondary structure<br />
<strong>Abstract</strong> : The conformation <strong>of</strong> peptide and protein drugs in various microenvironments and the interaction with drug carriers such as<br />
liposomes are <strong>of</strong> considerable interest. In this study the influence <strong>of</strong> microenvironments such as pH, salt concentration, and<br />
surface charge on the secondary structure <strong>of</strong> a model protein, lysozyme, either in solution or entrapped in liposomes with<br />
various molar ratios <strong>of</strong> phosphatidylcholine (PC):cholesterol (Chol) was investigated. It was found that entrapment efficiency<br />
was more pronounced in negatively charged liposomes than in non-charged liposomes, which was independent <strong>of</strong> Chol<br />
content and pH <strong>of</strong> hydration medium. The occurrence <strong>of</strong> aggregation, decrease in zeta potential, and alteration <strong>of</strong> 31P NMR<br />
chemical shift <strong>of</strong> negatively charged lysozyme liposomes compared to blank liposomes suggested that the electrostatic<br />
interaction plays a major role in protein-lipid binding. Addition <strong>of</strong> sodium chloride could impair the neutralizing ability <strong>of</strong><br />
positively charged lysozyme on negatively charged membrane via chloride counterion binding. Neither lysozyme in various<br />
buffer solutions with sodium chloride nor that entrapped in liposomes showed any significant change in their secondary<br />
structures. However, significant decrease in α-helical content <strong>of</strong> lysozyme in non-charged liposomes at higher pH and salt<br />
concentrations was discovered. © 2009.<br />
No.1693<br />
Author (s) : Wittawatmongkol, O., Vanprapar, N., Chearskul, P., Phongsamart, W., Prasitsuebsai, W., Sutthent, R., Chokephaibulkit, K.<br />
Title : Boosted p24 antigen assay for early diagnosis <strong>of</strong> perinatal HIV infection<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 2, February <strong>2010</strong>, Pages 187-190<br />
Document Type : Review<br />
Keywords : Boosted-p24 antigen; Diagnostic test; HIV-exposed infants<br />
<strong>Abstract</strong> : Objective: The authors evaluated the accuracy <strong>of</strong> in-house boosted-p24 antigen assay for diagnosis <strong>of</strong> perinatal HIV infection.<br />
Material and Method: The author has retrospectively reviewed the medical records <strong>of</strong> infants born to HIVpositive mothers.<br />
The infants were tested for boosted-p24 antigen assay at the age <strong>of</strong> 1-2 months and 4-6 months. HIV infection was defined as<br />
positive anti-HIV at the age 18 months or older, or had positive HIV-PCR with clinical signs and symptoms compatible with<br />
HIV/AIDS. Results: There were 168 infants included in this review and six were HIV-infected. The boosted-p24 antigen assay<br />
had the sensitivity, specificity, positive predictive value, and negative predictive value <strong>of</strong> 33.33%, 98.27%, 50%, and 95.8%,<br />
respectively at 1-2 month-old, and 100%, 98.27%, 71.43%, and 100%, respectively at 4-6 month-old. Conclusion: Boosted-p24<br />
antigen assay could be a cheaper alternative test to help diagnosis <strong>of</strong> perinatal HIV infection in infants. The test was very<br />
accurate when performed at 4-6 months.<br />
No.1694<br />
Author (s) : Wittayacom, K., Uthaipibull, C., Kumpornsin, K., Tinikul, R., Kochakarn, T., Songprakhon, P., Chookajorn, T.<br />
Title : A nuclear targeting system in Plasmodium falciparum<br />
Source : MALARIA JOURNAL, Volume: 9, Article Number: 126, DOI: 10.1186/1475-2875-9-126, Published: MAY 14 <strong>2010</strong><br />
Document Type : Article<br />
Keywords : VAR GENE-EXPRESSION; MALARIA PARASITE; DNA-BINDING; PROTEINS; YEAST; GAL4; LOCALIZATION; DISCOVERY; VIRULENCE;<br />
LOCATION<br />
<strong>Abstract</strong> : Background: The distinct differences in gene control mechanisms acting in the nucleus between Plasmodium falciparum and<br />
the human host could lead to new potential drug targets for anti-malarial development. New molecular toolkits are required<br />
for dissecting molecular machineries in the P. falciparum nucleus. One valuable tool commonly used in model organisms is<br />
protein targeting to specific sub-cellular locations. Targeting proteins to specified locations allows labeling <strong>of</strong> organelles for<br />
microscopy, or testing <strong>of</strong> how the protein <strong>of</strong> interest modulates organelle function. In recent years, this approach has been<br />
developed for various malaria organelles, such as the mitochondrion and the apicoplast. A tool for targeting a protein <strong>of</strong><br />
choice to the P. falciparum nucleus using an exogenous nuclear localization sequence is reported here. Methods: To develop<br />
a nuclear targeting system, a putative nuclear localization sequence was fused with green fluorescent protein (GFP). The<br />
nuclear localization sequence from the yeast transcription factor Gal4 was chosen because <strong>of</strong> its well-defined nuclear<br />
localization signal. A series <strong>of</strong> truncated Gal4 constructs was also created to narrow down the nuclear localization sequence<br />
necessary for P. falciparum nuclear import. Transfected parasites were analysed by fluorescent and laser-scanning confocal<br />
microscopy. Results: The nuclear localization sequence <strong>of</strong> Gal4 is functional in P. falciparum. It effectively transported GFP<br />
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into the nucleus, and the first 74 amino acid residues were sufficient for nuclear localization. Conclusions: The Gal4 fusion<br />
technique enables specific transport <strong>of</strong> a protein <strong>of</strong> choice into the P. falciparum nucleus, and thus provides a tool for<br />
labeling nuclei without using DNA-staining dyes. The finding also indicates similarities between the nuclear transport<br />
mechanisms <strong>of</strong> yeast and P. falciparum. Since the nuclear transport system has been thoroughly studied in yeast, this could<br />
give clues to research on the same mechanism in P. falciparum.<br />
No.1695<br />
Author (s) : Wittayarat, M., Thongphakdee, A., Saikhun, K., Chatdarong, K., Otoi, T., Techakumphu, M.<br />
Title : Cell cycle analysis <strong>of</strong> cultured skin fibroblasts from the Asian golden cat (Pard<strong>of</strong>elis temminckii), marbled cat (Pard<strong>of</strong>elis<br />
marmorata), leopard (Panthera pardus) and Siamese cat (Felis Catus)<br />
Source : Thai Journal <strong>of</strong> Veterinary Medicine, Volume 40, Issue 1, March <strong>2010</strong>, Page 139<br />
Document Type : Article<br />
Keywords : Cell cycle; Contact inhibition; Feline; Roscovitine; Serum starvation<br />
<strong>Abstract</strong> : –<br />
No.1696<br />
Author (s) : Wittonpanich, R.; Pulkes, T.; Dejthevaporn, C.; Yodnopklao, P.; Witoonpanich, P.; Wetchaphanphesat, S.; Brengman, J.; Engel, A.<br />
Title : Mutation <strong>of</strong> alpha subunit <strong>of</strong> acetylcholine receptor causing slow-channel congenital myasthenic syndrome in a Thai family<br />
Source : NEUROMUSCULAR DISORDERS, Volume: 20, Issue: 9-10, Pages: 630-630, DOI:10.1016/j.nmd.<strong>2010</strong>.07.111, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1697<br />
Author (s) : Wiwatanapataphee, B., Noinang, S., Wu, Y.H., Nuntadilok, B.<br />
Title : An integrated powerpoint-maple based teaching-learning model for multivariate integral calculus<br />
Source : <strong>International</strong> Electronic Journal <strong>of</strong> Mathematics Education, Volume 5, Issue 1, February <strong>2010</strong>, Pages 5-31<br />
Document Type : Article<br />
Keywords : Graphic visualization; Integral calculus; Maple; Symbolic package<br />
<strong>Abstract</strong> : In this paper, we present and evaluate a symbolic package based teaching-learning model for triple integral calculus. The<br />
model utilizes presentation and visualization technologies to assist in the teaching and learning <strong>of</strong> mathematical concepts<br />
and methods. The key mathematical concepts and integration techniques for triple integrals are introduced to students<br />
utilizing the animation feature <strong>of</strong> PowerPoint display supported by the graphic visualization function <strong>of</strong> the Maple s<strong>of</strong>tware.<br />
To enhance students' learning process, a series <strong>of</strong> workbooks and symbolic tools are developed utilizing Maple 10 to guide<br />
students step-by-step in their studies. The developed symbolic tools also enable students to display the three-dimensional<br />
(3D) integration domain graphically and to check their calculation results. An investigation undertaken shows that with the<br />
proposed teaching-learning model, a higher percentage <strong>of</strong> students achieved a better understanding <strong>of</strong> the concepts and a<br />
better capacity in evaluating triple integrals with complicated integration domains, compared to traditional teaching and<br />
learning with no technology use. We purport that our technology model supports understanding by showing the 3D figures in<br />
integration from different angles, and also facilitates independent learning by reducing the need to draw these figures by the<br />
students themselves.<br />
No.1698<br />
Author (s) : Wiwatanaratanabutr, I., Allan, S., Linthicum, K., Kittayapong, P.<br />
Title : Strain-specific differences in mating, oviposition, and host-seeking behavior between Wolbachia-infected and uninfected<br />
Aedes albopictus<br />
Source : Journal <strong>of</strong> the American Mosquito Control Association, Volume 26, Issue 3, September <strong>2010</strong>, Pages 265-273<br />
Document Type : Article<br />
Keywords : Aedes albopictus; host-seeking; mating; oviposition; Wolbachia<br />
<strong>Abstract</strong> : Wolbachia are maternally inherited bacteria that cause various reproductive alterations in their arthropod hosts, including<br />
cytoplasmic incompatibility. In this study, we compared mating, oviposition, and host-seeking behavior <strong>of</strong> Wolbachia-infected<br />
(Houston HOU, Gainesville GNV) and Houston uninfected (HT1) Aedes albopictus. In mating assays with virgin mosquitoes,<br />
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mating success <strong>of</strong> Wolbachia-infected males was significantly higher than uninfected strains. Mating success was highest with<br />
HOU males exposed to infected (95) and uninfected females (100), and lowest with HT1 males exposed to infected (20) and<br />
uninfected (25) females. Results suggested that Wolbachia infection may influence the reproductive behavior <strong>of</strong> this<br />
mosquito. There were no clear differences in oviposition responses between strains, with all strains ovipositing significantly<br />
more <strong>of</strong>ten on hay infusion and larval rearing water than on water controls and least frequently on 4-methylphenol. Strains<br />
<strong>of</strong> Ae. albopictus females were host-seeking a human when given a choice. Responses to a human arm, acetone, CO2, and<br />
dichloromethane were generally higher from the Houston strains than from the GNV strain. Responses <strong>of</strong> HOU and HT1<br />
females differed from GNV with greater responses to the arm and CO2. © <strong>2010</strong> by The American Mosquito Control<br />
Association, Inc.<br />
No.1699<br />
Author (s) : Wiyaporn, K., Tocharoenchai, C., Pusuwan, P., Ekjeen, T., Leaungwutiwong, S., Thanyarak, S.<br />
Title : Factors affecting standardized uptake value (SUV) <strong>of</strong> positron emission tomography (PET) imaging with18F-FDG<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 1, January <strong>2010</strong>, Pages 108-114<br />
Document Type : Article<br />
Keywords : 18F-FDG; Factors affecting; Positron emission tomography (PET); Standardized uptake value (SUV)<br />
<strong>Abstract</strong> : Objective: The purpose <strong>of</strong> the study was to study factors affecting SUV <strong>of</strong> PET imaging with 18F-FDG. Material and Method:<br />
PET/CT Biograph 64 was used to acquire the data. A NEMA PET phantom with 6 spheres varying in diameter from 10 to 37<br />
mm was used to mimic the human body and tumors. Background activity <strong>of</strong> 18F in the phantom was 0.14 μCi/ml and tumorto-background<br />
ratios (TBR) <strong>of</strong> 2:1, 5:1 and 10:1 were studied. For each TBR, thirty sinograms were acquired with 3-min scan<br />
durations. Different scan durations varying from 3 to 20 min using a TBR <strong>of</strong> 5:1were studied and three datasets <strong>of</strong> each scan<br />
time were collected. Sinograms were reconstructed using the Ordered Subset Expectation Maximization (OSEM) algorithm<br />
with 5 mm Full-Width-at-Half-Maximum (FWHM) Gaussian filtering. Sinograms at TBR <strong>of</strong> 5:1 were reconstructed by varying the<br />
number <strong>of</strong> iterative updates <strong>of</strong> OSEM (N) from 8 to 168 and SUVavg and SUVmax were measured. The percentage <strong>of</strong><br />
underestimation <strong>of</strong> SUVs was used to study the effect <strong>of</strong> tumor size and TBR. Intraclass correlation coefficient (ICC) was used<br />
to test the reliability <strong>of</strong> SUVmax with different scan durations. Results: The results showed that both the SUVavg and SUVmax<br />
rapidly increased when N was < 48 and slightly increased afterwards. At TBRs ranging from 2:1 to 10:1, the percentages <strong>of</strong><br />
underestimation <strong>of</strong> SUVmax ranged from 8.17 to 22.46 and that <strong>of</strong> SUVavg were ranged from 41.44 to 52.33 for 37-mm sphere<br />
and from 40.38 to 54.52 and from 48.97 to 67.73 for 10-mm sphere respectively. Different scan durations gave reliable<br />
SUVsmax with ICC <strong>of</strong> 0.996. Conclusion: SUVs increased as N increased. The percentage <strong>of</strong> underestimation <strong>of</strong> the SUV<br />
depended on tumor size and TBR. Scan duration did not affect SUVs.<br />
No.1700<br />
Author (s) : Wongchitrat, P.; Mukda, S.; Phansuwan-Pujito, P.; Govitrapong, P.<br />
Title : EFFECTS OF AMPHETAMINE ON CIRCADIAN GENES EXPRESSION IN THE RAT BRAIN<br />
Source : JOURNAL OF NEUROCHEMISTRY, Volume: 115, Pages: 12-12, Supplement: 1, Published: OCT <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1701<br />
Author (s) : Wongchoosuk, C., Wisitsoraat, A., Phokharatkul, D., Tuantranont, A., Kerdcharoen, T.<br />
Title : Multi-walled carbon nanotube-doped tungsten oxide thin films for hydrogen gas sensing<br />
Source : Sensors, Volume 10, Issue 8, August <strong>2010</strong>, Pages 7705-7715<br />
Document Type : Article<br />
Keywords : Carbon nanotube; E-beam evaporation; Hydrogen sensor; Nanochannels; WO3<br />
<strong>Abstract</strong> : In this work we have fabricated hydrogen gas sensors based on undoped and 1 wt% multi-walled carbon nanotube (MWCNT)doped<br />
tungsten oxide (WO3) thin films by means <strong>of</strong> the powder mixing and electron beam (E-beam) evaporation technique.<br />
Hydrogen sensing properties <strong>of</strong> the thin films have been investigated at different operating temperatures and gas<br />
concentrations ranging from 100 ppm to 50,000 ppm. The results indicate that the MWCNT-doped WO3 thin film exhibits high<br />
sensitivity and selectivity to hydrogen. Thus, MWCNT doping based on E-beam co-evaporation was shown to be an effective<br />
means <strong>of</strong> preparing hydrogen gas sensors with enhanced sensing and reduced operating temperatures. Creation <strong>of</strong><br />
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nanochannels and formation <strong>of</strong> p-n heterojunctions were proposed as the sensing mechanism underlying the enhanced<br />
hydrogen sensitivity <strong>of</strong> this hybridized gas sensor. To our best knowledge, this is the first report on a MWCNT-doped WO3<br />
hydrogen sensor prepared by the E-beam method. © <strong>2010</strong> by the authors.<br />
No.1702<br />
Author (s) : Wongchoosuk, C., Wisitsoraat, A., Tuantranont, A., Kerdcharoen, T.<br />
Title : Portable electronic nose based on carbon nanotube-SnO2 gas sensors and its application for detection <strong>of</strong> methanol<br />
contamination in whiskeys<br />
Source : Sensors and Actuators, B: Chemical, Volume 147, Issue 2, 3 June <strong>2010</strong>, Pages 392-399<br />
Document Type : Article<br />
Keywords : Carbon nanotube; E-nose; Feature extraction techniques; Gas sensor; Metal oxide<br />
<strong>Abstract</strong> : In this paper, a portable electronic nose (E-nose) based on hybrid carbon nanotube-SnO2 gas sensors is described. The hybrid<br />
gas sensors were fabricated using electron beam (E-beam) evaporation by means <strong>of</strong> powder mixing. The instrument employs<br />
feature extraction techniques including integral and primary derivative, which lead to higher classification performance as<br />
compared to the classical features (∆R and ∆R/R0). It was shown that doping <strong>of</strong> carbon nanotube (CNT) improves the<br />
sensitivity <strong>of</strong> hybrid gas sensors, while quantity <strong>of</strong> CNT has a direct effect on the selectivity to volatile organic compounds,<br />
i.e., methanol (MeOH) and ethanol (EtOH). The real-world applications <strong>of</strong> this E-nose were also demonstrated. Based on the<br />
proposed methods, this instrument can monitor and classify 1 vol% <strong>of</strong> MeOH contamination in whiskeys. © <strong>2010</strong> Elsevier B.V.<br />
All rights reserved.<br />
No.1703<br />
Author (s) : Wongdee, K., Riengrojpitak, S., Krishnamra, N., Charoenphandhu, N.<br />
Title : Claudin expression in the bone-lining cells <strong>of</strong> female rats exposed to long-standing academia<br />
Source : Experimental and Molecular Pathology, Volume 88, Issue 2, April <strong>2010</strong>, Pages 305-310<br />
Document Type : Article<br />
Keywords : Acidosis; Bone membrane; Claudin; Immunohistochemistry; Osteoblasts; Tight junction<br />
<strong>Abstract</strong> : Besides enhancing osteoclast-mediated bone resorption, chronic metabolic acidosis (CMA) induces mineral efflux across the<br />
epithelial-like bone membrane formed by bone-lining cells (inactive osteoblasts), possibly via the paracellular pathway.<br />
However, there was a compensatory mechanism that restricted bone loss in the late phase <strong>of</strong> CMA, and changes in the<br />
expression <strong>of</strong> claudins, which are tight junction proteins known to regulate epithelial barrier function, were therefore<br />
anticipated in bone-lining cells. Herein, primary rat osteoblasts were found to express several transcripts <strong>of</strong> claudins, i.e.,<br />
claudin-5, -11, -14, -15 and -16. Their protein expressions in bone-lining cells were demonstrated by immunohistochemistry in<br />
decalcified tibial sections. After exposure to CMA induced by oral administration <strong>of</strong> 1.5% NH4Cl for 21 days, expression <strong>of</strong><br />
claudin-14, which normally seals the paracellular space and restricts ion movement, was increased, whereas that <strong>of</strong> claudin-<br />
15 and -16 which form pores for ion transport were decreased. Expressions <strong>of</strong> claudin-5 and -11 were not changed by CMA. In<br />
conclusion, the bone-lining cells <strong>of</strong> rats exposed to CMA for 21 days upregulated an ion-restrictive claudin (i.e., claudin-14),<br />
while downregulating ion-permeable claudins (i.e., claudin-15 and -16). These cellular responses might be parts <strong>of</strong> a<br />
compensatory mechanism accounting for deceleration <strong>of</strong> bone loss in late CMA. © 2009 Elsevier Inc.<br />
No.1704<br />
Author (s) : Wongkamchai, S., Khongtak, P., Leemingsawat, S., Komalamisra, N., Junsong, N., Kulthanan, K., Wisuthsarewong, W., Boitano, J.J.<br />
Title : Comparative identification <strong>of</strong> protein pr<strong>of</strong>iles and major allergens <strong>of</strong> saliva, salivary gland and whole body extracts <strong>of</strong><br />
mosquito species in Thailand<br />
Source : Asian Pacific Journal <strong>of</strong> Allergy and Immunology, Volume 28, Issue 2-3, June <strong>2010</strong>, Pages 162-169<br />
Document Type : Article<br />
Keywords : Mosquito allergens; Mosquito bite allergy; Saliva; Salivary gland<br />
<strong>Abstract</strong> : Allergic reactions to mosquito bites, such as generalized urticaria or severe local reactions are common problems worldwide.<br />
The diverse sources <strong>of</strong> allergen prepared from different mosquito body parts usage are a major obstacle to obtaining safe and<br />
effective tests and immunotherapy for mosquito bite allergy. Thus, the reactions are <strong>of</strong>ten not recognized and allergen<br />
immunotherapy is seldom used for severe reaction to mosquito bites. In a search for appropriate allergen sources, the<br />
protein pr<strong>of</strong>iles <strong>of</strong> saliva, salivary glands and whole body extracts were comparatively analyzed from 4 common mosquito<br />
species <strong>of</strong> Thailand and/or South East Asia; viz. Culex quinquefasciatus, Aedes aegypti, Aedes albopictus and a zoophilic<br />
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strain, Anopheles minimus. The major allergens in the extracts which elicited specific IgE responses in the pooled sera <strong>of</strong><br />
subjects allergic to mosquito bites were identified. It was concluded that mosquito saliva was the best source <strong>of</strong> allergens.<br />
Additionally, both species-specific and species-shared allergens <strong>of</strong> the 4 mosquito species were identified. The major saliva<br />
allergens having MWs <strong>of</strong> 36, 32 and 22 kDa were identified. The identificstion <strong>of</strong> major allergens should facilitate the<br />
production <strong>of</strong> specific recombinant allergens and contribute to improvement in the diagnosis and specific immunotherapy <strong>of</strong><br />
Thai mosquito bite allergy patients.<br />
No.1705<br />
Author (s) : Wongkia, W., Naruedonkul, K., Cercone, N.<br />
Title : Thai visually impaired's requirements to access mathematics via an automatic math reader<br />
Source : CSEDU <strong>2010</strong> - 2nd <strong>International</strong> Conference on Computer Supported Education, Proceedings, Volume 1, <strong>2010</strong>, Pages 239-244<br />
Document Type : Conference Paper<br />
Keywords : Blind and visually impaired learning; Math expression; Text-to-speech<br />
<strong>Abstract</strong> : We present the requirements to access mathematics for the Thai visually impaired students. Blind and visually impaired (VI)<br />
students agreed to participate in an interview to determine the requirements for using a text-to-speech (ITS) system with the<br />
capability to automatically read math expressions. The interview consisted <strong>of</strong> two parts: the students' background<br />
characteristics and the students' difficulties in and requirement for using TTS systems to read math expressions. Our results<br />
show that the students who are blind and visually impaired had difficulties to access mathematics. The students have basic<br />
computer skills with standard s<strong>of</strong>tware e.g., Micros<strong>of</strong>t Word. Therefore, the findings suggest that the students desire assistant<br />
technology to better access to mathematics.<br />
No.1706<br />
Author (s) : Wongkongkatep, J., Ladadat, R., Lappermpunsap, W., Wongkongkatep, P., Phinyocheep, P., Ojida, A., Hamachi, I.<br />
Title : Thermoresponsive fluorescent sensor based on core/shell nanocomposite composed <strong>of</strong> gold nanoparticles and poly(Nisopropylacrylamide)<br />
Source : Chemistry Letters, Volume 39, Issue 3, <strong>2010</strong>, Pages 184-185<br />
Document Type : Article<br />
Keywords : –<br />
<strong>Abstract</strong> : We developed a new thermometric fluorescent sensor based on core/shell nanocomposite composed <strong>of</strong> gold nanoparticles<br />
and thermoresponsive poly(N-isopropylacrylamide), which shows a reversible fluorescence change in response to a<br />
temperature change. © <strong>2010</strong> The Chemical Society <strong>of</strong> Japan.<br />
No.1707<br />
Author (s) : Wongniam, S., Somana, J., Swangpol, S., Seelanan, T., Chareonsap, P., Chadchawan, S., Jenjittikul, T.<br />
Title : Genetic diversity and species-specific PCR-based markers from AFLP analyses <strong>of</strong> Thai bananas<br />
Source : Biochemical Systematics and Ecology, Volume 38, Issue 3, June <strong>2010</strong>, Pages 416-427<br />
Document Type : Article<br />
Keywords : AFLP; DNA fingerprinting; Genetic diversity; Interspecific hybrids; Musa cultivars<br />
<strong>Abstract</strong> : A large amount <strong>of</strong> banana genetic resource has been found in Thailand which is believed to be one <strong>of</strong> the centers <strong>of</strong> its<br />
origins. To assess genetic diversity and determine genetic relationships <strong>of</strong> edible bananas in Thailand, 110 accessions <strong>of</strong><br />
banana species and cultivars collected from villages and natural locations were investigated. UPGMA clustering <strong>of</strong> numerical<br />
data from Amplified Fragment Length Polymorphism (AFLP) patterns showed two large groups which corresponded to<br />
genome designations <strong>of</strong> Musa acuminata (AA) and Musa balbisiana (BB), the known ancestors <strong>of</strong> most edible cultivars. The<br />
AFLP data suggested that among Thai bananas, AA and AAA cultivars were closely related to M. acuminata subsp.<br />
malaccensis, while some <strong>of</strong> 'B' genome contained ones closely related to wild M. balbisiana in Thailand and some may have<br />
been imported. Eight species-specific PCR-based primer pairs, generated from the AFLP results clearly identify 'A' and 'B'<br />
genomes within cultivars and hybrids. The analyses were useful to readily and easily infer progenitors <strong>of</strong> these cultivars and<br />
pronounce wide genetic diversity <strong>of</strong> the bananas in Thailand. © <strong>2010</strong> Elsevier Ltd. All rights reserved.<br />
No.1708<br />
Author (s) : Wongprajun, S., Auewarakul, C.U.<br />
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Title : A method comparison study <strong>of</strong> flow cytometry and cytomorphology to determine the percentages <strong>of</strong> blasts in patients with<br />
acute leukemia after induction and consolidation chemotherapy.<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand = Chotmaihet thangphaet, Volume 93 Suppl 1, January <strong>2010</strong>, Pages S157-164<br />
Document Type : Article<br />
Keywords : antineoplastic agent, CD45 antigen; acute granulocytic leukemia, acute lymphoblastic<br />
leukemia, adolescent, adult, aged, article, bone marrow cell, cell count, comparative study, cytology, female, flow<br />
cytometry, human, leukemia, male, methodology, middle aged, minimal residual disease, remission;<br />
Adolescent, Adult, Aged, Antigens, CD45, Antineoplastic Agents, Bone Marrow Cells, Cell Count, Female, Flow<br />
Cytometry, Humans, Leukemia, Leukemia, Myeloid, Acute, Male, Middle Aged, Neoplasm, Residual, Precursor Cell<br />
Lymphoblastic Leukemia-Lymphoma, Remission Induction, Young Adult<br />
<strong>Abstract</strong> : BACKGROUND: Enumeration <strong>of</strong> blasts in the bone marrow is an essential component in the diagnosis and treatment <strong>of</strong> acute<br />
leukemia. The current gold standard method is based on a morphologic counting <strong>of</strong> 500 marrow nucleated cells despite its<br />
operator dependence and inter-observer variability. OBJECTIVES: To compare the percentages <strong>of</strong> marrow blasts derived from<br />
two different approaches comprising routine morphology-based manual counting and flow cytometric analysis. MATERIAL AND<br />
METHOD: Fifty-five marrow samples were collected from 38 acute leukemia patients (36 AML and 19 ALL) after hematologic<br />
recovery from chemotherapy. The blast percentages were enumerated manually and by flow cytometer using CD45 and side<br />
scatter gates. RESULTS: A good correlation was found in the overall 55 samples (r = 0.829) and 36 AML samples (r = 0.86). The<br />
blast percentages derived from flow cytometer were higher than from morphologic counting in 46 samples (83.6%). Using a<br />
cut-<strong>of</strong>f point <strong>of</strong> < 5% blasts to define complete remission (CR), 48 cases (87%) were classified as morphological CR (83% CR in<br />
AML and 95% CR in ALL). By flow cytometry, only 24 cases (44%) were in CR (28% CR in AML and 74% CR in ALL). The results<br />
from each method were concordant in determining CR in 27 samples (49%), with a kappa value <strong>of</strong> 0.07 for overall samples,<br />
0.057 for AML and -0.096 for ALL samples. CONCLUSION: A good correlation between the percentages <strong>of</strong> blasts achieved by<br />
either method was demonstrated, particularly in AML samples. Discordant results occurred when
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
with a mean age (standard deviation [SD]) <strong>of</strong> 42.2 (10.2) years and 77% were males. Median (range) duration <strong>of</strong> ART and<br />
lamivudine use was 46 (3-177) and 40 (3-140) months, respectively. Median (range) CD4 cell count was 352 (49-790) cells/mm<br />
3. Of all, 19 (23%) had HBV-DR with a median (range) HBV DNA <strong>of</strong> 2.56 × 107 (2.54 × 103-11 × 107) IU/mL. In univariate<br />
analysis, there were no differences in age, gender, ART regimen, liver function test, anti-HBc antibody, anti-HCV antibody<br />
between the two groups. Patients with HBV-DR had a higher proportion <strong>of</strong> positive HBeAg (68.4% versus 3.8%, p < 0.001). In<br />
multivariate analysis, positive HBeAg (odds ratio [OR) 16.64; 95% confidence interval [CI], 3.31-83.60] and duration <strong>of</strong><br />
lamivudine use [per 6-month increment, OR 1.24; 95% CI, 1.06-1.36] were significant risk factors for HBV-DR. All 19 patients<br />
with HBV-DR had lamivudine resistance with the mutations as follows: M204V/I (95%), L180M/A181T (95%), L80V/I (47%),<br />
V173L (32%), and N236T (21%). Of these, 95%, 84%, 84%, and 0% <strong>of</strong> patients had HBV-DR to telbivudine, entecavir, adefovir,<br />
and ten<strong>of</strong>ovir, respectively. HBV-DR is common in HBV/HIV-1 coinfected patients receiving lamivudine-containing ART without<br />
ten<strong>of</strong>ovir. Positive HBeAg and longer duration <strong>of</strong> lamivudine use are risk factors for HBV-DR. In addition to lamivudine<br />
resistance, cross-resistance to other anti-HBV drugs is also frequently observed. © Mary Ann Liebert, Inc.<br />
No.1711<br />
Author (s) : Wongsamitkul, N., Sirianant, L., Muanprasat, C., Chatsudthipong, V.<br />
Title : A plant-derived hydrolysable tannin inhibits CFTR chloride channel: A potential treatment <strong>of</strong> diarrhea<br />
Source : Pharmaceutical Research, Volume 27, Issue 3, March <strong>2010</strong>, Pages 490-497<br />
Document Type : Article<br />
Keywords : CFTR; Chloride secretion; Diarrhea; Tannin<br />
<strong>Abstract</strong> : Purpose: The present study examined the effects and mechanisms <strong>of</strong> actions <strong>of</strong> penta-m-digalloyl-glucose (PDG), a<br />
hydrolysable tannin extracted from Chinese gallnut, on cystic fibrosis transmembrane conductance regulator protein (CFTR).<br />
Materials and Methods: Fisher rat thyroid cells stably expressing human CFTR (FRT cells) and human intestinal T84 cells were<br />
used as cell models to investigate the effects <strong>of</strong> PDG on chloride secretion using short-circuit current analysis. The<br />
mechanisms by which PDG affected chloride secretion were also examined. Finally, in vivo antidiarrheal efficacy and effects<br />
<strong>of</strong> PDG on intestinal fluid absorption were evaluated in mouse closed-loop models. Results: In FRT cells, apical chloride<br />
current induced by forskolin, CPT-cAMP and apigenin were reversibly inhibited by PDG (IC50 ∼ 10μM) without effects on<br />
intracellular cAMP content and cell viability. Similarly, in T84 cells, PDG effectively inhibited chloride secretion induced by<br />
forskolin and cholera toxin. However, it had no effect on calcium-induced chloride secretion. In mice, a single intraluminal<br />
injection <strong>of</strong> PDG (0.6 mg/kg) reduced cholera toxin-induced intestinal fluid secretion by 75% with no effect on intestinal fluid<br />
absorption. Conclusions: PDG represents a new class <strong>of</strong> CFTR inhibitors. Further development <strong>of</strong> this class <strong>of</strong> compounds may<br />
provide a new therapeutic intervention for diarrhea. © 2009 Springer Science+Business Media, LLC.<br />
No.1712<br />
Author (s) : Wongsantichon, J., Robinson, R.C., Ketterman, A.J.<br />
Title : Structural contributions <strong>of</strong> Delta class glutathione transferase active-site residues to catalysis<br />
Source : Biochemical Journal, Volume 428, Issue 1, 15 May <strong>2010</strong>, Pages 25-32<br />
Document Type : Article<br />
Keywords : Crystal structure; Glutathione transferase (GST); Structural motif; Structure-function relationship; Substrate specificity<br />
<strong>Abstract</strong> : GST (glutathione transferase) is a dimeric enzyme recognized for biotransformation <strong>of</strong> xenobiotics and endogenous toxic<br />
compounds. In the present study, residues forming the hydrophobic substrate-binding site (H-site) <strong>of</strong> a Delta class enzyme<br />
were investigated in detail for the first time by site-directed mutagenesis and crystallographic studies. Enzyme kinetics reveal<br />
that Tyr111 indirectly stabilizes GSH binding, Tyr119 modulates hydrophobic substrate binding and Phe123 indirectly<br />
modulates catalysis. Mutations at Tyr111 and Phe123 also showed evidence for positive co-operativity for GSH and 1-chloro-<br />
2,4- dinitrobenzene respectively, strongly suggesting a role for these residues in manipulating subunit-subunit communication.<br />
In the present paper we report crystal structures <strong>of</strong> the wild-type enzyme, and two mutants, in complex with Shexylglutathione.<br />
This study has identified an aromatic 'zipper' in the H-site contributing a network <strong>of</strong> aromatic π-π<br />
interactions. Several residues <strong>of</strong> the cluster directly interact with the hydrophobic substrate, whereas others indirectly<br />
maintain conformational stability <strong>of</strong> the dimeric structure through the C-terminal domain (domain II). The Y119E mutant<br />
structure shows major main-chain rearrangement <strong>of</strong> domain II. This reorganization is moderated through the 'zipper' that<br />
contributes to the H-site remodelling, thus illustrating a role in co-substrate binding modulation. The F123A structure shows<br />
molecular rearrangement <strong>of</strong> the H-site in one subunit, but not the other, explaining weakened hydrophobic substrate binding<br />
and kinetic co-operativity effects <strong>of</strong> Phe123 mutations. The three crystal structures provide comprehensive evidence <strong>of</strong> the<br />
624
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
aromatic 'zipper' residues having an impact upon protein stability, catalysis and specificity.Consequently, 'zipper' residues<br />
appear to modulate and co-ordinate substrate processing through permissive flexing. © The Authors.<br />
No.1713<br />
Author (s) : Wongtrakul, J., Pongjaroenkit, S., Leelapat, P., Nachaiwieng, W., Prapanthadara, L.-A., Ketterman, A.J.<br />
Title : Expression and characterization <strong>of</strong> three new glutathione transferases, an epsilon (AcGSTE2-2), Omega (AcGSTO1-1), and Theta<br />
(AcGSTT1-1) from anopheles cracens (Diptera: Culicidae), a major thai malaria vector<br />
Source : Journal <strong>of</strong> Medical Entomology, Volume 47, Issue 2, February <strong>2010</strong>, Pages 162-171<br />
Document Type : Article<br />
Keywords : Anopheles cracens; DDT dehydrochlorinase; Epsilon class; Omega class; Theta class<br />
<strong>Abstract</strong> : Glutathione transferases (GSTs) (E.C.2.5.1.18) are multifunctional enzymes involved in the detoxification <strong>of</strong> many exogenous<br />
and endogenous compounds. This study aimed to characterize several new GSTs from Anopheles cracens, a major Thai<br />
malaria vector formerly known as Anopheles dirus. The three recombinant enzymes obtained were from the epsilon, theta<br />
and omega classes. They showed 8093% identity to orthologous An. gambiae GSTs. AcGSTE2-2 possessed peroxidase activity<br />
that cannot be detected for the An. gambiae AgGSTE2-2. AcGSTT1-1 had high activity toward several substrates that are<br />
specific for mammalian theta class. The AcGSTO1-1 can use 1-chloro-2,4-dinitrobenzene, dichloroacetic acid, and<br />
hydroxyethyl disulfide substrates. The enzymes bound but did not metabolize the organophosphate temephos. The epsilon<br />
AcGSTE2-2 functioned as a peroxidase and DDT metabolizing enzyme. The theta AcGSTT1-1 functioned not only as<br />
peroxidase but also acted as a binding protein for organophosphates. The omega GST had thiol transferase activity suggesting<br />
a role in oxidative stress response. © <strong>2010</strong> Entomological Society <strong>of</strong> America.<br />
No.1714<br />
Author (s) : Wongwananuruk, T., Indhavivadhana, S., Rattanachaiyanont, M., Techatraisak, K., Leerasiri, P., Tanmahasamut, P., Surasak, A.,<br />
Dangrat, C.<br />
Title : Characteristics <strong>of</strong> 250 reproductive-aged polycystic ovary syndrome Thai women at Siriraj Hospital<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand, Volume 93, Issue 4, April <strong>2010</strong>, Pages 399-405<br />
Document Type : Article<br />
Keywords : Characteristics; PCOS; Polycystic ovary syndrome; Thai<br />
<strong>Abstract</strong> : Objective: To determine the clinical characteristics <strong>of</strong> reproductive-aged polycystic ovary syndrome (PCOS) Thai women.<br />
Study design: Cross sectional study. Settings: Gynecologic Endocrinology Unit, Department <strong>of</strong> Obstetrics and Gynecology,<br />
Faculty <strong>of</strong> Medicine Siriraj Hospital. Subjects: 250 PCOS Thai women who registered at the Siriraj PCOS project from May 2007<br />
to January 2009. PCOS were diagnosed using Revised Rotterdam 2003 criteria. Women who were taking medications affecting<br />
sex hormones or lipid metabolism within 3 months before registration were excluded from the present study. Material and<br />
Method: Patients were interviewed and examined for weight, height, waist circumference, blood pressure, presence <strong>of</strong><br />
acanthosis nigricans, and signs <strong>of</strong> hyperandrogenism. Ovarian ultrasonography was examined using vaginal probe inserting into<br />
the vagina or rectum. Venous blood sample <strong>of</strong> each patient was drawn during 8.00-10.00 o'clock after 12-hour fasting. Main<br />
outcome measures: Clinical characteristics and laboratory pr<strong>of</strong>iles in PCOS Thai women. Results: Of all participants, 62% were<br />
20-29.9 years old, 30% had high blood pressure, 57% were overweight to obese, 49% had central obesity, and 27% had<br />
acanthosis nigricans. Clinical hyperandrogenism was found in 15.6% <strong>of</strong> the patients. Approximately 7% <strong>of</strong> PCOS women had<br />
impaired fasting glucose and one third had dyslipidemia. Prevalence <strong>of</strong> the PCOS criteria presenting in the population were<br />
oligomenorrhea and/or amenorrhea (98.4%), hyperandrogenism (49.2%), and ultrasonographic polycystic ovary (97.2%). Of all<br />
participants, 44% had three components <strong>of</strong> diagnostic criteria. Among those who had two components, presence <strong>of</strong> abnormal<br />
menstrual cycle plus polycystic ovary was the most common finding. Conclusion: Menstrual problem was the most common<br />
presenting symptom among the presented participants. Hyperandrogenism/ -emia adds only a little value on making PCOS<br />
diagnosis. Most <strong>of</strong> the PCOS Thai women have menstrual problem. In these patients, ovarian ultrasonography has high value<br />
to diagnose PCOS; addition <strong>of</strong> androgen blood test can diagnose only 3% more PCOS cases. Although the presented PCOS<br />
Thai women are still young, approximately 50% already have some parameters <strong>of</strong> health risk. It is suggested to provide<br />
preventive measures for these patients to prevent long term medical problems.<br />
No.1715<br />
Author (s) : Wongwattanasathien, O., Kangsadalampai, K., Tongyonk, L.<br />
Title : Antimutagenicity <strong>of</strong> some flowers grown in Thailand<br />
625
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Source : Food and Chemical Toxicology, Volume 48, Issue 4, April <strong>2010</strong>, Pages 1045-1051<br />
Document Type : Article<br />
Keywords : 1-Aminopyrene; Antimutagens; Flower; Nitrite; Salmonella typhimurium<br />
<strong>Abstract</strong> : The mutagenicity <strong>of</strong> dichloromethane, methanol and water extracts <strong>of</strong> Antigonon leptopus Hook. & Arn., Curcuma sessilis<br />
Gage, Hibiscus rosa-sinensis Linn., Ixora coccinea Linn., Millingtonia hortensis Linn., Nelumbo nucifera Gaertn., Plumeria obtusa<br />
Linn., Punica granatum Linn., Rhinacanthus nasutus ((Linn.) Kurz.) and Syzygium malaccense ((Linn.) Merr.& Perry) before and<br />
after nitrite treatment was firstly investigated in the Ames test. Their antimutagenicity against the product <strong>of</strong> the reaction<br />
mixture <strong>of</strong> 1-aminopyrene nitrite model in the absence <strong>of</strong> metabolic activation on Salmonella typhimurium TA 98 and TA 100<br />
was evaluated. The results showed that none <strong>of</strong> the samples was mutagenic. Most nitrite-treated samples but<br />
dichloromethane extracts <strong>of</strong> Hibiscus rosa-sinensis, Plumeria obtusa, Syzygium malaccense, methanol extract <strong>of</strong> Syzygium<br />
malaccense and water extract <strong>of</strong> Hibiscus rosa-sinensis were mutagenic. The nitrite treated methanol extract <strong>of</strong> Nelumbo<br />
nucifera exhibited the highest mutagenicity on both strains. All dichloromethane extracts <strong>of</strong> flowers decreased the<br />
mutagenicity induced by the product <strong>of</strong> 1-aminopyrene nitrite model on both tester strains. Methanol extract <strong>of</strong> Curcuma<br />
sessilis and Punica granatum (15 mg/plate) showed the highest antimutagenic activity in TA 98 and TA 100, respectively. The<br />
protective effects <strong>of</strong> these flower extracts might be due to the presence <strong>of</strong> antimutagenic components that were supposed<br />
to be flavonoids. © <strong>2010</strong> Elsevier Ltd. All rights reserved.<br />
No.1716<br />
Author (s) : Wongwerawattanakoon, P.; Chuansumrit, A.; Angchaisuksiri, P.; Sirachainan, N.<br />
Title : Education package for patients with hemophilia and family members at the <strong>International</strong> Training Center-Bangkok: an effective<br />
model using limited resources<br />
Source : HAEMOPHILIA, Volume: 16, Pages: 53-53, Supplement: 4, Published: JUL <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1717<br />
Author (s) : Wongwitthayakool, P.; Sirisinha, C.; Saeoui, P.<br />
Title : Cure and Viscoelastic Properties <strong>of</strong> HNBR Effects <strong>of</strong> Carbon Black Loading and Characteristics<br />
Source : KGK-KAUTSCHUK GUMMI KUNSTSTOFFE, Volume: 63, Issue: 11, Pages: 506-512, Published: NOV <strong>2010</strong><br />
Document Type : Article<br />
Keywords : Reinforcement; Viscoelastic properties; Carbon black; Dynamic mechanical properties; Hydrogenated acrylonitrile butadiene<br />
rubber<br />
<strong>Abstract</strong> : Cure, viscoelastic and mechanical properties <strong>of</strong> HNBR filled with various types <strong>of</strong> carbon black were investigated. By increasing<br />
carbon black loading and specific surface area, cure promotion was observed which could be explained by a combination <strong>of</strong><br />
thermal history, surface chemistry and thermal conductivity. Viscoelastic behaviour <strong>of</strong> both uncured and cured specimens<br />
filled with carbon black exhibits a strain-dependent behaviour. Storage modulus (G') and damping factor (tans) significantly<br />
increase with increasing carbon black loading and/or specific surface area. Mechanical properties are found to be governed by<br />
combined effects <strong>of</strong> hydrodynamic effect, filler transient network, molecular slippage and crosslink density, associated<br />
probably with black dispersion particularly at high black loading. The overall results imply a close correlation <strong>of</strong> viscoelastic<br />
and mechanical properties via energy dissipation process (or hysteretic process) caused by molecular slippage.<br />
No.1718<br />
Author (s) : Worachartcheewan, A., Nantasenamat, C., Isarankura-Na-Ayudhya, C., Pidetcha, P., Prachayasittikul, V.<br />
Title : Identification <strong>of</strong> metabolic syndrome using decision tree analysis<br />
Source : Diabetes Research and Clinical Practice, Volume 90, Issue 1, October <strong>2010</strong>, Pages e15-e18<br />
Document Type : Article<br />
Keywords : Cardiovascular diseases; Data mining; Decision tree; Diabetes; Health status; Metabolic syndrome<br />
<strong>Abstract</strong> : This study employs decision tree as a decision support system for rapid and automated identification <strong>of</strong> individuals with<br />
metabolic syndrome (MS) among a Thai population. Results demonstrated strong predictivity <strong>of</strong> the decision tree in<br />
classification <strong>of</strong> individuals with and without MS, displaying an overall accuracy in excess <strong>of</strong> 99%. © <strong>2010</strong> Elsevier Ireland Ltd.<br />
626
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.1719<br />
Author (s) : Worachartcheewan, A., Nantasenamat, C., Isarankura-Na-Ayudhya, C., Pidetcha, P., Prachayasittikul, V.<br />
Title : Lower BMI cut<strong>of</strong>f for assessing the prevalence <strong>of</strong> metabolic syndrome in Thai population<br />
Source : Acta Diabetologica, Volume 47, Issue SUPPL. 1, December <strong>2010</strong>, Pages S91-S96<br />
Document Type : Article<br />
Keywords : BMI; Cardiovascular disease; Diabetes; IDF; Metabolic syndrome<br />
<strong>Abstract</strong> : This article investigates the prevalence <strong>of</strong> metabolic syndrome (MS) and the benefits <strong>of</strong> lowered body mass index (BMI) cut<strong>of</strong>f<br />
point for assessing MS prevalence in a large, nationally representative population sample comprising <strong>of</strong> 15,365 Thai adults<br />
from metropolitan Bangkok who received annual checkup. Prevalence <strong>of</strong> MS was characterized using the <strong>International</strong><br />
Diabetes Federation criteria and BMI 25 kg/m2 as cut<strong>of</strong>f revealed that 26.63% <strong>of</strong> male and 14.90% <strong>of</strong> female subjects had MS<br />
and the prevalence was age dependent. Traditional BMI cut<strong>of</strong>f <strong>of</strong> 30 kg/m2 underestimated MS prevalence in Thai population<br />
while BMI 25 kg/m2 was found to be a suitable solution. Common combinations <strong>of</strong> MS components were identified in order<br />
to find common occurrences that may be implicated in the development <strong>of</strong> diabetes and/or cardiovascular diseases. © 2009<br />
Springer-Verlag.<br />
No.1720<br />
Author (s) : Worasuwannarak, S., Pornratanarangsi, S.<br />
Title : Prediction <strong>of</strong> contrast-induced nephropathy in diabetic patients undergoing elective cardiac catheterization or PCI: role <strong>of</strong><br />
volume-to-creatinine clearance ratio and iodine dose-to-creatinine clearance ratio.<br />
Source : Journal <strong>of</strong> the Medical Association <strong>of</strong> Thailand = Chotmaihet thangphaet, Volume 93 Suppl 1, January <strong>2010</strong>, Pages S29-34<br />
Document Type : Article<br />
Keywords : contrast medium, creatinine, iodine, topical antiinfective agent; aged, angiocardiography, area under the<br />
curve, article, blood, chemically induced disorder, diabetic angiopathy, dose response, female, heart<br />
catheterization, human, incidence, kidney disease, male, methodology, middle aged, postoperative period, prediction and<br />
forecasting, prospective study, risk factor, transluminal coronary angioplasty, treatment outcome; Aged, Aged, 80 and<br />
over, Angioplasty, Transluminal, Percutaneous Coronary, Anti-Infective Agents, Local, Area Under Curve, Contrast<br />
Media, Coronary Angiography, Creatinine, Diabetic Angiopathies, Dose-Response Relationship, Drug, Female, Heart<br />
Catheterization, Humans, Incidence, Iodine, Kidney Diseases, Male, Middle Aged, Postoperative Period, Predictive Value <strong>of</strong><br />
Tests, Prospective Studies, Risk Factors, Treatment Outcome<br />
<strong>Abstract</strong> : OBJECTIVE: To assess a role <strong>of</strong> volume-to-creatinine clearance ratio (V/CrCl) and iodine dose-to-creatinine clearance ratio (Idose/CrCl)<br />
in predicting contrast- induced nephropathy (CIN) in diabetic patients undergoing elective cardiac catheterization or<br />
percutaneous coronary intervention (PCI). BACKGROUND: In diabetic patients undergoing cardiac catheterization or PCI, the<br />
incidence <strong>of</strong> CIN is higher than in non-diabetic patients. High doses <strong>of</strong> contrast media also increase the likelihood <strong>of</strong> renal<br />
dysfunction. The ratio <strong>of</strong> the volume <strong>of</strong> contrast media to creatinine clearance (V/CrCl) and iodine dose-to-creatinine<br />
clearance (I-dose/CrCl) has been shown to correlate with the area under the curve <strong>of</strong> contrast media concentration over time<br />
and was used to predict the occurrence <strong>of</strong> CIN in unselected patients. No study has been conducted specifically in diabetic<br />
patients undergoing cardiac catheterization or PCI before. MATERIAL AND METHOD: We conducted a prospective, single center<br />
study. The V/CrCl and I-dose/CrCl were calculated in diabetic patients undergoing elective cardiac catheterization or PCI. An<br />
increase in serum creatinine <strong>of</strong> > 0.5 mg/dl or > 25% by 7 days from baseline was considered CIN. The incidence <strong>of</strong> CIN was<br />
determined. The predictive value <strong>of</strong> V/CrCl and I-dose/CrCl for CIN were assessed using multivariable logistic regression.<br />
RESULTS: The total number <strong>of</strong> patients that had been enrolled in the study was 248; Male 50.8%. The overall incidence <strong>of</strong><br />
CIN was 5.2%. The mean age for the entire population was 65 +/- 9 years; the mean body mass index was 25.6 +/- 4.0 kg/m2;<br />
and the mean creatinine clearance was 60.6 +/- 27.4 ml/min. The mean values <strong>of</strong> V/CrCl for patients with and without CIN<br />
were 3.7 +/- 2.9 and 2.2 +/- 1.7 (p = 0.041). The mean values <strong>of</strong> I-dose/CrCl for patients with and without CIN were 1.31 +/-<br />
0.94 and 0.82 +/- 0.63 (p = 0.042). The receiver-operator characteristic curve analysis indicated that a V/CrCl ratio <strong>of</strong> 2.60 and<br />
I-dose/CrCl <strong>of</strong> 0.98 were fair predictors <strong>of</strong> CIN. After adjusting for other known predictors <strong>of</strong> CIN, a V/CrCl ratio > or = 2.60<br />
remained the only significant predictor <strong>of</strong> CIN (Odds ratio 5.8; 95% confidence interval 1.7-19.4, p = 0.005). CONCLUSIONS: A<br />
V/CrCl ratio > or = 2.60 was a significant predictor <strong>of</strong> CIN in diabetic patients undergoing elective cardiac catheterization or<br />
PCI.<br />
No.1721<br />
Author (s) : Woravutrangkul, S., Jarungjitaree, S., Sritara, C., Vachalathiti, R., Chuaychoo, B.<br />
627
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Efficacy <strong>of</strong> pursed lips breathing with forced expiration techniques and active cycle <strong>of</strong> breathing technique on pulmonary<br />
mucus clearance in healthy subjects<br />
Source : Journal <strong>of</strong> Physical Therapy Science, Volume 22, Issue 3, <strong>2010</strong>, Pages 247-254<br />
Document Type : Article<br />
Keywords : Active cycle <strong>of</strong> breathing technique; Pursed lips breathing with forced expiration techniques; Radioaerosol tracer technique<br />
<strong>Abstract</strong> : [Purpose] To investigate the efficacy <strong>of</strong> pursed lips breathing with forced expiration techniques (PLB & FETs) and the active<br />
cycle <strong>of</strong> breathing technique (ACBT) on pulmonary mucus clearance in healthy subjects. [Methods] Three healthy subjects<br />
volunteered for the study. Pulmonary mucus clearance was measured over 70 minutes on three different days by a<br />
radioaerosol technique. Each subject attended three sessions, one each <strong>of</strong> control (normal breathing), PLB & FETs and ACBT.<br />
[Results] The efficacy <strong>of</strong> both airway clearance techniques was similar, but better than that <strong>of</strong> normal breathing. In the central<br />
lung zone, PLB & FETs demonstrated a slight enhancement <strong>of</strong> mucus clearance as compared with ACBT and normal<br />
breathing. In the intermediate and peripheral zones, PLB & FETs and ACBT resulted in a relatively high mucus clearance as<br />
compared with normal breathing. Overall mucus clearance <strong>of</strong> normal lungs was remarkably improved by PLB & FETs and<br />
ACBT. [Conclusion] Enhanced mucus clearance in healthy subjects, particularly in the intermediate and peripheral lung zone,<br />
can be achieved by PLB & FETs and ACBT.<br />
No.1722<br />
Author (s) : Worawongvasu, R., Songkampol, K.<br />
Title : Fibro-osseous lesions <strong>of</strong> the jaws: An analysis <strong>of</strong> 122 cases in Thailand<br />
Source : Journal <strong>of</strong> Oral Pathology and Medicine, Volume 39, Issue 9, October <strong>2010</strong>, Pages 703-708<br />
Document Type : Article<br />
Keywords : clinicopathologic feature; fibro-osseous lesion; jaws; relative frequency; Thailand<br />
<strong>Abstract</strong> : Background: There are no previously published reports on fibro-osseous jaw lesions in Thailand. The aims <strong>of</strong> this study were<br />
to determine the relative frequencies and the clinicopathologic features <strong>of</strong> these lesions in Thailand, and to compare these<br />
data with information available in the literature. Methods: A total <strong>of</strong> 122 cases <strong>of</strong> fibro-osseous lesions <strong>of</strong> the jaws, out <strong>of</strong> a<br />
total <strong>of</strong> 4808 biopsy specimens, were collected from the files <strong>of</strong> the Department <strong>of</strong> Oral Pathology, Faculty <strong>of</strong> Dentistry,<br />
<strong>Mahidol</strong> <strong>University</strong>, Bangkok, Thailand, during a 34-year period from 1973 to 2006. Clinical data and histopathologic diagnoses<br />
were reviewed and analyzed. Results: The most common was ossifying fibroma (50.8%), followed by fibrous dysplasia (42.6%).<br />
Ossifying fibroma most frequently occurred in the third and fourth decades <strong>of</strong> life (61.3%) and mostly involved the posterior<br />
region <strong>of</strong> the mandible (41.9%). Fibrous dysplasia was mostly seen in the second decade <strong>of</strong> life (40.4%). The maxilla was<br />
involved far more <strong>of</strong>ten than the mandible (53.8% and 6.2%, respectively), most common in the posterior region <strong>of</strong> the<br />
maxilla (28.8%). Both lesions presented as painless swellings (62.9% and 90.4%, respectively). Radiographically, ossifying<br />
fibroma mostly was mixed radiolucent-radiopacity (45.2%). Fibrous dysplasia mostly appeared as a radiopaque lesion (34.6%).<br />
Conclusions: The relative frequency <strong>of</strong> osseous dysplasia is underestimated because most lesions are not treated and the<br />
patients are kept under long-term follow-up without biopsy. The clinicopathologic features <strong>of</strong> ossifying fibroma and fibrous<br />
dysplasia in Thailand are identical to those in the literature. © <strong>2010</strong> The Authors. Journal <strong>of</strong> Oral Pathology & Medicine ©<br />
<strong>2010</strong> John Wiley & Sons A/S.<br />
No.1723<br />
Author (s) : Wutthisathapornchai, A.; Boonsaen, T.; Wallace, J. C.; Jitrapakdee, S.<br />
Title : Identification <strong>of</strong> the Glucose Responsive Element(s) That Mediates Transcription Regulation <strong>of</strong> Rat Pyruvate Carboxylase.<br />
Source : ENDOCRINE REVIEWS, Volume: 31, Issue: 3, Supplement: 1, Published: JUN <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1724<br />
Author (s) : Wynne, A.<br />
Title : The Buddha's skill in means and the genesis <strong>of</strong> the five aggregate teaching winner <strong>of</strong> the 2nd pr<strong>of</strong>essor Mary Boyce award<br />
Source : Journal <strong>of</strong> the Royal Asiatic Society, Volume 20, Issue 2, April <strong>2010</strong>, Pages 191-216<br />
Document Type : Article<br />
Keywords : –<br />
628
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : The problem tackled in this article is ambitious. Through examination <strong>of</strong> how certain fundamental teachings <strong>of</strong> the Buddha<br />
originated - the author argues that those teachings must indeed go back to the Buddha himself. Thus the author builds a<br />
chain <strong>of</strong> argument which creates hypothetical links rather than declaring a priori that links and connection cannot be<br />
established. This article argues that the Alagaddpama Sutta, an important early Buddhist text, portrays the Buddha in the<br />
process <strong>of</strong> formulating his thoughts. If so it contradicts the myth that the Buddha awakened to the entire Buddhist Dharma on<br />
one occasion, and should be dated to the fourth century bce. Such an antiquity, and peculiar didactic structure suggests that<br />
the text contains authentic teachings <strong>of</strong> the Buddha. © <strong>2010</strong> The Royal Asiatic Society.<br />
No.1725<br />
Author (s) : Wynne, A.<br />
Title : WHAT THE BUDDHA THOUGHT<br />
Source : JOURNAL OF THE ROYAL ASIATIC SOCIETY, Volume: 20, Pages: 395-404, Part: Part 3, Published: JUL <strong>2010</strong><br />
Document Type : Book Review<br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1726<br />
Author (s) : Yafa, C., Srithongtim, S., Phukphatthanachai, P., Thiparuk, A., Sudsiri, N., Rojanapantip, L., Uraroongroj, M., Kluengklangdon, P.,<br />
Jaengkarnkit, P., Sirisuthanant, K., Thonglue, C., Pimma, T., Pengpreecha, P., Fortune, T.J., Zwicker, R., Permnamtip, V.,<br />
Laoharojanaphnad, S., Suanmamuang, B., Shiowatana, J., Waiyawat, W., Siripinyanond, A., Judprasong, K., Boonsong, B.,<br />
Talaluck, S., Cherdchu, C.<br />
Title : New reference material for analysis <strong>of</strong> elements in glutinous rice produced at the National Institute <strong>of</strong> Metrology (Thailand)<br />
Source : Accreditation and Quality Assurance, Volume 15, Issue 4, April <strong>2010</strong>, Pages 223-231<br />
Document Type : Article<br />
Keywords : Elements; Inorganic analysis; Reference material; Rice<br />
<strong>Abstract</strong> : Reference materials play an important role for evaluating the accuracy <strong>of</strong> analytical results, and are essential parts <strong>of</strong> good<br />
laboratory practice. They represent a key tool for quality control <strong>of</strong> chemical analyses. In Thailand, the demand <strong>of</strong> food and<br />
environmental reference materials is constantly increasing, and the National Institute <strong>of</strong> Metrology (NIMT, Thailand) is<br />
responding to the urgent needs for affordable materials, which require collaborative efforts at the national level. This paper<br />
describes the preparation <strong>of</strong> a new glutinous rice reference material, along with homogeneity and stability studies and the<br />
analytical work carried out for the certification <strong>of</strong> the contents <strong>of</strong> inorganic elements. The incurred material was collected<br />
from an actual rice paddy field. Material preparation along with homogeneity and stability testing were carried out at the<br />
Environmental Research Training Centre (ERTC). The homogeneity study was designed to have three experimental conditions;<br />
(A) 10 bottles <strong>of</strong> candidate materials being analyzed each with 2 replicates, (B) 20 bottles with 2 replicates, (C) 10 bottles with<br />
7 replicates, in order to study the suitable treatments for homogeneity testing in the reference material production. It was<br />
shown that a minimum number <strong>of</strong> 10 bottles with duplicate analyses are enough to demonstrate the homogeneity <strong>of</strong><br />
candidate reference material. Certification <strong>of</strong> a candidate reference material in a single laboratory using reference method<br />
was confirmed with an interlaboratory comparison participated by a certain number <strong>of</strong> well recognized testing laboratories in<br />
Thailand. Further elaborative results will be discussed. © Springer-Verlag 2009.<br />
No.1727<br />
Author (s) : Yamborisut, U., Sakamoto, N., Wimonpeerapattana, W., Tontisirin, K.<br />
Title : Waist circumference and body fat distribution indexes as screening tools for the overweight and obesity in Thai preschool<br />
children<br />
Source : Obesity Research and Clinical Practice, Volume 4, Issue 4, October-December <strong>2010</strong>, Pages e307-e314<br />
Document Type : Article<br />
Keywords : Body mass index; Preschool children; Thai; Total body fat; Trunk skinfold; Waist circumference<br />
<strong>Abstract</strong> : Background: Evidence shows that waist circumference (WC) is one reliable index to predict abdominal obesity in children. This<br />
study aims to examine the relationship <strong>of</strong> WC to other anthropometric indexes and to determine the ability <strong>of</strong> WC as obesity<br />
screening tool. Subjects: 811, 5-6 years old children in Saraburi province, central region <strong>of</strong> Thailand. Methods: Anthropometric<br />
measurements were performed in children; 406 boys and 405 girls. WC measurement was performed at the umbilicus level.<br />
Subcutaneous skinfold was measured on subscapular, suprailiac and abdominal regions. Total body fat was measured with<br />
629
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
bioelectrical impedance analyzer. Receiver operating characteristic (ROC) analysis was employed to determine WC cut-<strong>of</strong>fs for<br />
predicting obesity in children. Results: WC highly correlated with weight-for-height Z-score (WHZ) (r = 0.92-0.94, p = 0.01),<br />
body mass index (BMI) (r = 0.95-0.96, p = 0.01), trunk skinfold (r = 0.92-0.93, p = 0.01) and total body fat (r = 0.94-0.95, p =<br />
0.01) for both genders. Based on Thai national reference, the optimal WC cut-<strong>of</strong>fs for predicting obesity were 59.6 cm for boys<br />
and 60.5 cm for girls. When IOTF-BMI was employed as reference, WC thresholds were 64.4 cm for boys and 63.1 cm for girls.<br />
The latter WC cut-<strong>of</strong>fs provided the slightly underestimated obesity prevalence compared with national reference.<br />
Conclusion: The strongly positive correlation between WC and weight-height based index and between WC and body fat in<br />
Thai preschool children suggests that WC should be the additional index for obesity screening in young children. Further<br />
study needs to explore the association between the increased WC and other adverse health outcomes. © <strong>2010</strong> Asian<br />
Oceanian Association for the Study <strong>of</strong> Obesity.<br />
No.1728<br />
Author (s) : Yanatatsaneeji, P., Kitkumthorn, N., Dhammawipark, C., Rabalert, J., Patel, V., Mutirangura, A.<br />
Title : Codon72 polymorphism in the p53 tumor suppressor gene in oral lichen planus lesions in a Thai population<br />
Source : Asian Pacific Journal <strong>of</strong> Cancer Prevention, Volume 11, Issue 4, <strong>2010</strong>, Pages 1137-1141<br />
Document Type : Article<br />
Keywords : Apoptosis; Oral lichen planus; p53 codon 72; Polymorphism<br />
<strong>Abstract</strong> : Background: Oral lichen planus (OLP) is a T-cell mediated autoimmune disease in which autocytotoxic CD8+ T cells (CTLs)<br />
trigger apoptosis <strong>of</strong> oral epithelial cells. Activated CTLs can produce Fas ligand and by binding to Fas lead to apoptosis. This<br />
Fas pathway and the action <strong>of</strong> p53 tumour suppressor gene are important in producing apoptosis. Current data demonstrate<br />
a link between these two factors at the transcriptional level. Objective: The purpose <strong>of</strong> this study was to investigate the p53<br />
polymorphism at codon 72 which results in encoding <strong>of</strong> either proline or arginine. Methods: Our study used 97 OLP cases and<br />
94 control blood samples from non-OLP individuals and performed PCR-RFLP. Results: Compared to control individuals, we<br />
found a significant increase in occurrence in OLP patients <strong>of</strong> the proline encoding cytosine allele (adjusted odd ratio (95%<br />
CI)=2.29 (1.42-3.70) and p=0.001). In addition, in individuals with the non-erosive type <strong>of</strong> OLP, the same situation was evident<br />
(OR=2.29, 95% CI (1.38-3.78), p=0.001). Furthermore, we noted a significantly higher prevalence <strong>of</strong> homozygosity [OR=3.17,<br />
95% CI(1.58-7.25), p=0.001) for the p53 pro allele in the OLP group, which indicates a recessive mode <strong>of</strong> inheritance.<br />
Conclusion: Our data suggest a strong association between the pro/pro genotype and OLP, and that the process <strong>of</strong> apoptosis,<br />
in which p53 plays a role, is a factor in OLP pathogenesis.<br />
No.1729<br />
Author (s) : Yap, Y.-K., Smith, D.R.<br />
Title : Strategies for the plant-based expression <strong>of</strong> dengue subunit vaccines<br />
Source : Biotechnology and Applied Biochemistry, Volume 57, Issue 2, October <strong>2010</strong>, Pages 47-53<br />
Document Type : Short Survey<br />
Keywords : Bi<strong>of</strong>actory; Chloroplast expression system; Dengue; Plant-based expression; Transient expression; Vaccine<br />
<strong>Abstract</strong> : Despite significant efforts in many countries, there is still no commercially viable dengue vaccine. Currently, attention is<br />
focused on the development <strong>of</strong> either live attenuated vaccines or live attenuated chimaeric vaccines using a variety <strong>of</strong><br />
backbones. Alternate vaccine approaches, such as whole inactivated virus and subunit vaccines are in the early stages <strong>of</strong><br />
development, and are each associated with different problems. Subunit vaccines <strong>of</strong>fer the advantage <strong>of</strong> providing a uniform<br />
antigen <strong>of</strong> well-defined nature, without the added risk <strong>of</strong> introducing any genetic material into the person being inoculated.<br />
Preliminary trials <strong>of</strong> subunit vaccines (using dengue E protein) in rhesus monkeys have shown promising results. However, the<br />
primary disadvantages <strong>of</strong> dengue subunit vaccines are the low levels <strong>of</strong> expression <strong>of</strong> dengue proteins in mammalian or<br />
insect cells, as well as the added unknown risks <strong>of</strong> antigens produced from mammalian cells containing other potential<br />
sources <strong>of</strong> contamination. In the past two decades, plants have emerged as an alternative platform for expression <strong>of</strong><br />
biopharmaceutical products, including antigens <strong>of</strong> bacterial, fungal or viral origin. In the present minireview, we highlight the<br />
current plant expression technologies used for expression <strong>of</strong> biopharmaceutical products, with an emphasis on plants as a<br />
production system for dengue subunit vaccines. © <strong>2010</strong> Portland Press Limited.<br />
No.1730<br />
Author (s) : Yingchoncharoen, T., Limpijankit, T., Jongjirasiri, S., Laothammatas, J., Yamwong, S., Sritara, P.<br />
630
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
Title : Does arterial stiffness contribute to coronary artery disease risk prediction beyond the traditional risk score (RAMA-EGAT<br />
score)?<br />
Source : EUROPEAN HEART JOURNAL, Volume: 31, Pages: 1026-1027, Supplement: 1, Published: SEP <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1731<br />
Author (s) : Yiu, C.C.P., Chanplakorn, N., Chan, M.S.M., Loo, W.T.Y., Chow, L.W.C., Toi, M., Hironobu, S.<br />
Title : Down-regulation <strong>of</strong> heat-shock protein 70 (HSP-70) correlated with responsiveness to neoadjuvant aromatase inhibitor therapy<br />
in breast cancer patients<br />
Source : Anticancer Research, Volume 30, Issue 9, September <strong>2010</strong>, Pages 3465-3472<br />
Document Type : Article<br />
Keywords : Aromatase inhibitor; Breast cancer; Heat-shock protein 70; Ki-67; Neoadjuvant<br />
<strong>Abstract</strong> : Background: Aromatase inhibitor (AI) has been established as an effective endocrine therapy in estrogen receptor (ER)-positive<br />
postmenopausal breast cancer patients. Our recent proteomic analysis demonstrated that ten proteins were significantly<br />
altered in their expression levels before and after the therapy in the patients receiving neoadjuvant AI. Among these newly<br />
identified proteins, heat-shock protein 70 (HSP-70) was the most significantly correlated with both clinical and pathological<br />
responses. Therefore, in this study, we further evaluated the significance <strong>of</strong> this HSP-70 alteration using<br />
immunohistochemistry. Materials and Methods: A total <strong>of</strong> 32 patients treated with neoadjuvant exemestane or letrozole in<br />
whom pre- and post-treatment tumor tissues were available were included. Immunohistochemical evaluation <strong>of</strong> ER,<br />
progesterone receptor (PgR), Her-2, Ki-67 and HSP-70 was performed. Results obtained were compared to both clinical and<br />
biological responses <strong>of</strong> the patients. Results: The majority <strong>of</strong> the patients responded to treatment (16 patients with partial<br />
response, 14 with stable disease and 2 with progressive disease). The means <strong>of</strong> ER, Ki-67 and HSP-70 were significantly<br />
different between treatment responders and non-responders. Decrement <strong>of</strong> HSP-70 and Ki-67 after AI treatment and<br />
pretreatment Ki-67 labeling index <strong>of</strong> >10% tumor cells were significantly associated with clinical responsiveness to AI<br />
treatment (p
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
<strong>Abstract</strong> : ZnO nanoparticle-nanowire (NP-NW) array hybrid photoanodes for dye-sensitized solar cell (DSC) with NW arrays to serve as a<br />
direct pathway for fast electron transport and NPs dispersed between NWs to <strong>of</strong>fer a high specific surface area for sufficient<br />
dye adsorption has been fabricated and investigated to improve the power conversion efficiency (PCE). The overall PCE <strong>of</strong> the<br />
ZnO hybrid photoanode DSC with the N3-sensitized has reached ∼4.2%, much higher than both ∼1.58% <strong>of</strong> ZnO NW DSC<br />
and ∼1.31% <strong>of</strong> ZnO NP DSC, prepared and tested under otherwise identical conditions. © <strong>2010</strong> American Institute <strong>of</strong> Physics.<br />
No.1734<br />
Author (s) : Yodyingyong, S., Zhou, X., Zhang, Q., Triampo, D., Xi, J., Park, K., Limketkai, B., Cao, G.<br />
Title : Enhanced photovoltaic performance <strong>of</strong> nanostructured hybrid solar cell using highly oriented TiO2 nanotubes<br />
Source : Journal <strong>of</strong> Physical Chemistry C, Volume 114, Issue 49, 16 December <strong>2010</strong>, Pages 21851-21855<br />
Document Type : Article<br />
Keywords : Electron transfer, Fill factor, Hybrid solar cells, Identical conditions, Nano-structured, Photovoltaic performance, Power<br />
conversion efficiencies, Reactive deposition, TiO, ZnO nanorod; Conversion efficiency, Nanorods, Nanotubes, Open circuit<br />
voltage, Solar power generation, Zinc oxide; Solar cells<br />
<strong>Abstract</strong> : Highly oriented TiO2 nanotubes have been fabricated using ZnO nanorod template through liquid reactive deposition on the<br />
ITO substrates. The diameter and length <strong>of</strong> TiO2 nanotubes can be effectively controlled for the suitable use for a hybrid<br />
solar cell by varying the diameter and length <strong>of</strong> the ZnO nanorod template. A mixture <strong>of</strong> P3HT/PCBM was infiltrated into the<br />
gaps between TiO2 nanotubes to form hybrid solar cells. The open circuit voltage, short circuit current density, fill factor, and<br />
power conversion efficiency <strong>of</strong> the hybrid solar cell using highly oriented TiO 2 nanotubes were 646 mV, 9.95 mA cm-2,<br />
51.6%, and 3.32%, respectively, much higher than 1.2% <strong>of</strong> hybrid solar cell based on ZnO nanorods tested under otherwise<br />
identical conditions and significantly higher than 0.7% <strong>of</strong> the same type hybrid solar cells reported in literature. The<br />
enhancement <strong>of</strong> the power conversion efficiency could be resulted from the highly oriented TiO2 nanotubes with smaller<br />
diameter and large specific surface area for the efficient electron transfer in hybrid solar cells. © <strong>2010</strong> American Chemical<br />
Society.<br />
No.1735<br />
Author (s) : Yojina, J., Ngamsaad, W., Nuttavut, N., Triampo, D., Lenbury, Y., Kanthang, P., Sriyab, S., Triampo, W.<br />
Title : Investigating flow patterns in a channel with complex obstacles using the lattice Boltzmann method<br />
Source : Journal <strong>of</strong> Mechanical Science and Technology, Volume 24, Issue 10, October <strong>2010</strong>, Pages 2025-2034<br />
Document Type : Article<br />
Keywords : Flow pattern; Lattice Boltzmann method; Strouhal number; Von Karman vortex street<br />
<strong>Abstract</strong> : In this work, mesoscopic modeling via a computational lattice Boltzmann method (LBM) is used to investigate the flow<br />
pattern phenomena and the physical properties <strong>of</strong> the flow field around one and two square obstacles inside a twodimensional<br />
channel with a fixed blockage ratio, β=1/4, centered inside a 2D channel, for a range <strong>of</strong> Reynolds numbers (Re)<br />
from 1 to 300. The simulation results show that flow patterns can initially exhibit laminar flow at low Re and then make a<br />
transition to periodic, unsteady, and, finally, turbulent flow as the Re get higher. Streamlines and velocity pr<strong>of</strong>iles and a<br />
vortex shedding pattern are observed. The Strouhal numbers are calculated to characterize the shedding frequency and flow<br />
dynamics. The effect <strong>of</strong> the layouts or configurations <strong>of</strong> the obstacles are also investigated, and the possible connection<br />
between the mixing process and the appropriate design <strong>of</strong> a chemical mixing system is discussed. © <strong>2010</strong> The Korean Society<br />
<strong>of</strong> Mechanical Engineers and Springer-Verlag Berlin Heidelberg.<br />
No.1736<br />
Author (s) : Yojina, J., Ngamsaad, W., Nuttavut, N., Triampo, D., Lenbury, Y., Triampo, W., Kanthang, P., Sriyab, S.<br />
Title : More realistic model for simulating min protein dynamics: Lattice Boltzmann method incorporating the role <strong>of</strong> nucleoids<br />
Source : <strong>International</strong> Journal <strong>of</strong> Computational and Mathematical Sciences, Volume 4, Issue 4, <strong>2010</strong>, Pages 177-182<br />
Document Type : Article<br />
Keywords : Cell division; Lattice Boltzmann method; Min proteins oscillation; Nucleoid<br />
<strong>Abstract</strong> : The dynamics <strong>of</strong> Min proteins plays a center role in accurate cell division. Although the nucleoids may presumably play an<br />
important role in prokaryotic cell division, there is a lack <strong>of</strong> models to account for its participation. In this work, we apply the<br />
lattice Boltzmann method to investigate protein oscillation based on a mesoscopic model that takes into account the<br />
nucleoid's role. We found that our numerical results are in reasonably good agreement with the previous experimental results<br />
On comparing with the other computational models without the presence <strong>of</strong> nucleoids, the highlight <strong>of</strong> our finding is that the<br />
632
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
local densities <strong>of</strong> MinD and MinE on the cytoplasmic membrane increases, especially along the cell width, when the size <strong>of</strong><br />
the obstacle increases, leading to a more distinct cap-like structure at the poles. This feature indicated the realistic pattern<br />
and reflected the combination <strong>of</strong> Min protein dynamics and nucleoid's role.<br />
No.1737<br />
Author (s) : Yokthongwattana, K., Sriariyanun, M., Ekaratcharoenchai, P., Svasti, J.<br />
Title : Characterization <strong>of</strong> fatty acids and proteins associated with the xanthophyll-enriched membrane fraction isolated from the<br />
thylakoid membranes <strong>of</strong> irradiance-stressed Dunaliella salina<br />
Source : Journal <strong>of</strong> Applied Phycology, Volume 22, Issue 2, April <strong>2010</strong>, Pages 147-155<br />
Document Type : Article<br />
Keywords : Dunaliella salina; Irradiance stress; Membrane fractionation; Xanthophyll; Zeaxanthin<br />
<strong>Abstract</strong> : It has been previously reported that a considerable amount <strong>of</strong> lutein and zeaxanthin could be fractionated, upon mild<br />
detergent treatment, from the thylakoid membranes <strong>of</strong> irradiance-stressed unicellular green alga, Dunaliella salina, into a<br />
yellow pellet fraction. Such membrane pellet was found to be devoid <strong>of</strong> chlorophylls and any known proteins <strong>of</strong><br />
photosynthesis but rather contained a significant amount <strong>of</strong> unknown polypeptides. It was speculated that this xanthophyllrich<br />
membrane pellet might originate from incomplete solubilization <strong>of</strong> the photoinhibited thylakoids by weak surfactants,<br />
due to extra rigidity imposed by the xanthophylls being directly imbedded into the lipid bilayer. In this study, we further<br />
characterized this membrane fraction by studying its associated proteins and fatty acid composition. Analysis by gas<br />
chromatography-mass spectrometry indicated that this yellow pellet membrane was enriched in saturated fatty acids,<br />
supporting the rigidity notion <strong>of</strong> the pellet. Protein identification by MALDI-TOF MS further revealed that at least 20 watersoluble<br />
proteins were found in association with this pellet. These proteins may originate from unspecific contamination <strong>of</strong><br />
abundant polypeptides co-precipitated with the membrane upon fractionation. Possible explanations regarding the nature <strong>of</strong><br />
this xanthophyll-rich membrane are also discussed. © 2009 Springer Science+Business Media B.V.<br />
No.1738<br />
Author (s) : Yong, H.-H., Foong, K., Borland, R., Omar, M., Hamann, S., Sirirassamee, B., Fong, G.T., Fotuhi, O., Hyland, A.<br />
Title : Support for and reported compliance among smokers with smoke-free policies in air-conditioned hospitality venues in<br />
Malaysia and Thailand: Findings from the international tobacco control Southeast Asia survey<br />
Source : Asia-Pacific Journal <strong>of</strong> Public Health, Volume 22, Issue 1, January <strong>2010</strong>, Pages 98-109<br />
Document Type : Article<br />
Keywords : Air-conditioned hospitality venues; Compliance; Restaurants; Smoke-free policy; Support<br />
<strong>Abstract</strong> : This study examined support for and reported compliance with smoke-free policy in air-conditioned restaurants and other<br />
similar places among adult smokers in Malaysia and Thailand. Baseline data (early 2005) from the <strong>International</strong> Tobacco<br />
Control Southeast Asia Survey (ITC-SEA), conducted face-to-face in Malaysia and Thailand (n = 4005), were used. Among those<br />
attending venues, reported total smoking bans in indoor air-conditioned places such as restaurants, c<strong>of</strong>fee shops, and karaoke<br />
lounges were 40% and 57% in Malaysia and Thailand, respectively. Support for a total ban in air-conditioned venues was high<br />
and similar for both countries (82% Malaysian and 90% Thai smokers who believed there was a total ban), but self-reported<br />
compliance with bans in such venues was significantly higher in Thailand than in Malaysia (95% vs 51%, P < .001). As<br />
expected, reporting a ban in air-conditioned venues was associated with a greater support for a ban in such venues in both<br />
countries. © <strong>2010</strong> APJPH.<br />
No.1739<br />
Author (s) : Yoopetch, C.<br />
Titel : The model <strong>of</strong> knowledge acquisition: The study <strong>of</strong> SMEs in the thai hospitality industry<br />
Source : Proceedings <strong>of</strong> World Academy <strong>of</strong> Science, Engineering and Technology, Volume 66, June <strong>2010</strong>, Pages 351-354<br />
Document Type : Article<br />
Keywords : Information technology support; Knowledge acquisition; Social network; Teamworking<br />
<strong>Abstract</strong> : The main purpose <strong>of</strong> this research was to test the model <strong>of</strong> knowledge acquisition. This study emphasized the knowledge<br />
management activities in the aspects <strong>of</strong> knowledge acquisition. In addition, the unit <strong>of</strong> analysis was the Small and Medium<br />
Enterprises (SMEs) in the hospitality industry in Thailand, providing more in-depth understanding about how SMEs acquired<br />
knowledge. There were four factors affecting knowledge acquisition, including information technology support, teamworking,<br />
social network and internal communication. The findings indicated that social network and teamworking had high influence<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
on knowledge acquisition respectively. The suggestions and directions for future research in the areas <strong>of</strong> knowledge<br />
management were also discussed.<br />
No.1740<br />
Author (s) : Yoovathaworn, K., Satethasilnuchai, K., Sirivarasai, J., Kaojarern, S.<br />
Title : Linking exposure to environmental pollutants with oxidative stress in Bangkok bus drivers<br />
Source : DRUG METABOLISM REVIEWS Volume: 42 Pages: 247-248 Supplement: 1 Published: AUG <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1741<br />
Author (s) : Yoswathana, N., Eshtiaghi, M.N., Ebadi, A.G.<br />
Title : Extraction <strong>of</strong> adenosine from Ganoderma lucidum using novel extraction technologies<br />
Source : Asian Journal <strong>of</strong> Chemistry Volume 22, Issue 3, <strong>2010</strong>, Pages 2349-2355<br />
Document Type : Article<br />
Keywords : Adenosine; Ganoderma lucidum; High hydrostatic pressure extraction; Supercritical carbon dioxide extraction; Ultrasonic<br />
assisted extraction<br />
<strong>Abstract</strong> : The aim <strong>of</strong> this research is to extract adenosine from Ganoderma lucidum (GL) using novel extraction techniques, including<br />
high hydrostatic pressure (HHP), ultrasonic assisted extraction (UAE), supercritical carbon dioxide (ScCO2) in comparison with<br />
maceration and Soxhlet. The results showed that the highest adenosine extraction could be achieved using HHP (1.86 mg/g<br />
dry sample at 2000 bar, 60 °C sample to solvent ratio <strong>of</strong> 1:60 and very short extraction time <strong>of</strong> 5 min) followed by Soxhlet<br />
(1.77 mg/g dry sample, 3 h extraction time). The effect <strong>of</strong> pressure and temperature during supercritical carbon dioxide<br />
extraction <strong>of</strong> adenosine were distinct at pressure higher than 400 bar and moderate temperature (0.40 mg/g dry sample at<br />
500 bar and 60 °C). The combination <strong>of</strong> maceration and ultrasonic at moderate temperature (60 °C) improved the<br />
extractability <strong>of</strong> adenosine up 1.01 mg/g dry sample.<br />
No.1742<br />
Author (s) : Yu, Ho Khek; Lakhtakia, S.; Leong, Ang Tiing; Vu, C. K. F.; Shin, J.; Frederick, D. Y.; Heng, Chong Vui; Akaraviputh, T.; Kongkam, P.;<br />
Khor, C.; Mesenas, S.; Tan, J.; Kumar, R.; Chian, Lim Wee; Doshi, Bhavesh Kishor; Omar, S.; Sachitanandan, S.; Abraham, G.;<br />
Kitiyakara, T.; Rantachu, T.; Philips, M.; Wong, J.<br />
Title : Does endoscopic ultrasound influence the management <strong>of</strong> pancreatic cystic lesions? An Asian multicentre study<br />
Source : JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY Volume: 25 Pages: A24-A24 Supplement: 2 Published: SEP <strong>2010</strong><br />
Document Type : Meeting <strong>Abstract</strong><br />
Keywords : –<br />
<strong>Abstract</strong> : –<br />
No.1743<br />
Author (s) : Zembowicz, A., Mandal, R.V., Choopong, P.<br />
Title : Melanocytic lesions <strong>of</strong> the conjunctiva<br />
Source : Archives <strong>of</strong> Pathology and Laboratory Medicine Volume 134, Issue 12, December <strong>2010</strong>, Pages 1785-1792<br />
Document Type : Article<br />
Keywords : tumor marker; article, cell proliferation, classification, conjunctiva tumor, human, melanocyte, melanoma, melanosis,<br />
metabolism, nevus, pathology; Cell Proliferation, Conjunctival Neoplasms, Humans, Melanocytes, Melanoma, Melanosis,<br />
Nevus, Tumor Markers, Biological<br />
<strong>Abstract</strong> : Context: Melanocytic proliferations are among the most common neoplasms <strong>of</strong> the conjunctiva. They <strong>of</strong>ten represent<br />
challenging lesions for pathologists unfamiliar with unique histologic features <strong>of</strong> melanocytic proliferations in this location and<br />
with nomenclature used by ophthalmologists. Objective: To comprehensively review clinical aspects, pathologic features, and<br />
management <strong>of</strong> melanocytic proliferations <strong>of</strong> the conjunctiva. Data Sources: Review <strong>of</strong> the literature and personal experience<br />
<strong>of</strong> the authors. Conclusions: Classification, state <strong>of</strong> the art, and practical aspects <strong>of</strong> pathology <strong>of</strong> melanocytic proliferations <strong>of</strong><br />
the conjunctiva are discussed.<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.1744<br />
Author (s) : Zhai, D.-D., Supaibulwatana, K., Zhong, J.-J.<br />
Title : Inhibition <strong>of</strong> tumor cell proliferation and induction <strong>of</strong> apoptosis in 95-D lung cancer cells by Drimartol A from hairy root<br />
cultures <strong>of</strong> Artemisia annua<br />
Source : Latin American Journal <strong>of</strong> Pharmacy, Volume 29, Issue 7, <strong>2010</strong>, Pages 1159-1165<br />
Document Type : Article<br />
Keywords : Apoptosis; Artemisia annua; Cytotoxicity; Plant hairy root culture; Sesquiterpene courmarin ether<br />
<strong>Abstract</strong> : Drimartol A (DA), a sesquiterpene courmarin ether, was isolated from the cultured hairy roots <strong>of</strong> A. annua for the first time,<br />
and no biological activity <strong>of</strong> DA has ever been reported. In this work, DA was shown to possess interesting cytotoxic activities<br />
against the human tumor cell lines <strong>of</strong> HO8910 (ovary), 95-D (lung), QGY (liver) and HeLa (cervix) by MTT assay, whose IC50<br />
values were ranged within 17.94-22.3 μM for 24h. Given that treatment <strong>of</strong> lung cancer is a priority <strong>of</strong> our interest, induction <strong>of</strong><br />
apoptosis by DA in the human lung tumor cell line 95-D was focused. The 95-D cell growth was inhibited in a time<br />
dependent manner and its cell cycle was arrested in the G2 phase by DA. The apoptotic rate <strong>of</strong> the cells increased in a dosedependent<br />
manner. DA also increased the activity <strong>of</strong> caspase-9 and -3 and caused a decrease in the mitochondrial<br />
membrane potential. These results revealed that DA could efficiently induce 95-D cell apoptosis through mitochondrial<br />
dependent pathway, and it may be a potential chemotherapeutic agent.<br />
No.1745<br />
Author (s) : Zhai, D.-D., Supaibulwatana, K., Zhong, J.-J.<br />
Title : Inhibition <strong>of</strong> tumor cell proliferation and induction <strong>of</strong> apoptosis in human lung carcinoma 95-D cells by a new sesquiterpene<br />
from hairy root cultures <strong>of</strong> Artemisia annua<br />
Source : Phytomedicine, Volume 17, Issue 11, September <strong>2010</strong>, Pages 856-861<br />
Document Type : Article<br />
Keywords : Apoptosis; Artemisia annua; Cytotoxicity; Plant hairy root culture; Sesquiterpenoid<br />
<strong>Abstract</strong> : Artemisia annua is a rich source <strong>of</strong> many bioactive substances, and in our recent work, a new sesquiterpene, (Z)-7-acetoxymethyl-11-methyl-3-methylene-dodeca-1,6,10-triene<br />
(AMDT), was isolated and identified from hairy roots culture <strong>of</strong> A. annua,<br />
and its bioactivity was characterized in this work. AMDT showed moderate cytotoxic activities against the human tumor cell<br />
lines <strong>of</strong> HO8910 (ovary), 95-D (lung), QGY (liver) and HeLa (cervix) by MTT assay, whose IC50 values were ranged within 52.44-<br />
73.3μM. As lung cancer is the No. 1 killer <strong>of</strong> global cancer patients, our interest is to investigate the ability <strong>of</strong> AMDT in<br />
inducing apoptosis <strong>of</strong> 95-D tumor cells. The 95-D cell growth was inhibited by AMDT, and the flow cytometry analysis showed<br />
its cell cycle was arrested in the G1 phase. The apoptotic rate <strong>of</strong> the cells increased in a dose-dependent manner. AMDT<br />
lowered the mitochondrial membrane potential and increased the expression <strong>of</strong> caspase-9 and -3. These results revealed<br />
that AMDT could efficiently induce 95-D cell apoptosis through mitochondrial dependent pathway, and it may be a potential<br />
chemotherapeutic agent. © <strong>2010</strong> Elsevier GmbH.<br />
No.1746<br />
Author (s) : Zhong, Y., Krisanapun, C., Lee, S.-H., Nualsanit, T., Sams, C., Peungvicha, P., Baek, S.J.<br />
Title : Molecular targets <strong>of</strong> apigenin in colorectal cancer cells: Involvement <strong>of</strong> p21, NAG-1 and p53<br />
Source : European Journal <strong>of</strong> Cancer, Volume 46, Issue 18, December <strong>2010</strong>, Pages 3365-3374<br />
Document Type : Article<br />
Keywords : Apigenin; Colorectal cancer; NAG-1; p21; p53; PKCδ<br />
<strong>Abstract</strong> : Persuasive epidemiological and experimental evidence suggests that dietary flavonoids have anti-cancer activity. Since<br />
conventional therapeutic and surgical approaches have not been able to fully control the incidence and outcome <strong>of</strong> most<br />
cancer types, including colorectal neoplasia, there is an urgent need to develop alternative approaches for the management<br />
<strong>of</strong> cancer. We sought to develop the best flavonoids for the inhibition <strong>of</strong> cell growth, and apigenin (flavone) proved to be the<br />
most promising compound in colorectal cancer cell growth arrest. Subsequently, we found that pro-apoptotic proteins (NAG-<br />
1 and p53) and cell cycle inhibitor (p21) were induced in the presence <strong>of</strong> apigenin, and kinase pathways, including PKCδ and<br />
ataxia telangiectasia mutated (ATM), play an important role in activating these proteins. The data generated by in vitro<br />
experiments were confirmed in an animal study using APC MIN+ mice. Apigenin is able to reduce polyp numbers,<br />
accompanied by increasing p53 activation through phosphorylation in animal models. Our data suggest apparent beneficial<br />
effects <strong>of</strong> apigenin on colon cancer. © <strong>2010</strong> Elsevier Ltd. All rights reserved.<br />
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<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
No.1747<br />
Author (s) : Zhou, J., Li, P.C.K., Kumarasamy, N., Boyd, M., Chen, Y.M.A., Sirisanthana, T., Sungkanuparph, S., Oka, S., Tau, G., Phanuphak, P.,<br />
Saphonn, V., Zhang, F.J., Omar, S.F.S., Lee, C.K.C., Ditangco, R., Merati, T.P., Lim, P.L., Choi, J.Y., Law, M.G., Pujari, S.<br />
Title : Deferred modification <strong>of</strong> antiretroviral regimen following documented treatment failure in Asia: Results from the TREAT Asia<br />
HIV Observational Database (TAHOD)<br />
Source : HIV Medicine, Volume 11, Issue 1, <strong>2010</strong>, Pages 31-39<br />
Document Type : Article<br />
Keywords : Antiretroviral treatment; Asia Pacific region; Observational cohort; Treatment failure<br />
<strong>Abstract</strong> : Objective: The aim <strong>of</strong> the study was to examine the rates and predictors <strong>of</strong> treatment modification following combination<br />
antiretroviral therapy (cART) failure in Asian patients with HIV enrolled in the TREAT Asia HIV Observational Database (TAHOD).<br />
Methods: Treatment failure (immunological, virological and clinical) was defined by World Health Organization criteria.<br />
Countries were categorized as high or low income by World Bank criteria. Results: Among 2446 patients who initiated cART,<br />
447 were documented to have developed treatment failure over 5697 person-years (7.8 per 100 person-years). A total <strong>of</strong> 253<br />
patients changed at least one drug after failure (51.6 per 100 person-years). There was no difference between patients from<br />
high- and low-income countries [adjusted hazard ratio (HR) 1.02; . P=0.891]. Advanced disease stage [Centers for Disease<br />
Control and Prevention (CDC) category C . vs. A; adjusted HR 1.38, . P=0.040], a lower CD4 count (≥51 cells/μL . vs. ≤50<br />
cells/μL; adjusted HR 0.61, . P=0.022) and a higher HIV viral load (≥400 HIV-1 RNA copies/mL . vs.
<strong>Mahidol</strong> <strong>University</strong> <strong>Abstract</strong> <strong>of</strong> <strong>International</strong> <strong>Publications</strong> <strong>2010</strong><br />
continues. The effect on long-term outcomes through the possible development <strong>of</strong> HIV drug resistance remains uncertain. ©<br />
<strong>2010</strong> Zhou et al; licensee BioMed Central Ltd.<br />
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