DISSERTATION

_____________________________________________________________ Results and Discussion
reorganization of the DNA at the surface. Pulsing for 5 min creates a much stronger effect on
the DNA film inducing a significant decrease of Rct, which is most likely connected to DNA
desorption.
Figure 3.46. CV measurements made before and after surface modification with a) MCH
and b) MCU and subsequent FSCV measurements made with c) MCH- and d) MCUmodified
electrodes before and after incubation in Fc-tDNA solution. SAM formation was
done using the potential-pulse assisted method (0.5/-0.2 V vs. Ag/AgCl/3 M KCl, 10 ms
pulse duration) for 1 min in 1 mM thiol solution (10 mM PB, 20 mM K2SO4, 30 % ethanol).
CV measurements were performed in 10 mM PB, 20 mM K2SO4 containing 5 mM of
K3[Fe(CN)6] and K4[Fe(CN)6] at 100 mV/s scan rate. FSCV measurements were
performed in 10 mM PB with 450 mM K2SO4 at 1 V/s scan rate.
3.4 Potential-assisted preparation of DNA sensors 87