DISSERTATION
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5.1 Materials and consumables …..……………………………...…….......… 109<br />
5.2 Electrochemical setup and instrumentation ……………...……….…. 110<br />
5.3 Preparation of gold surfaces ….………………………………………. 111<br />
5.4 Determination of the potential of zero charge …...………………….. 113<br />
5.5 Potential-assisted formation of self-assembled monolayers ….…….. 114<br />
5.6 Potential-assisted desorption …………………………………………. 115<br />
5.7 Preparation of DNA sensors ….………………………………………. 116<br />
5.7.1 ssDNA immobilization via incubation ...…………………………………………… 116<br />
5.7.2 Potential-assisted ssDNA immobilization ….……………………………………… 116<br />
5.7.3 Passivation by means of incubation ...……………………………………………… 117<br />
5.7.4 Potential-assisted passivation .……………………………………………………… 117<br />
5.8 Preparation of DNA chips ..…………………………………………………… 117<br />
5.9 Characterization of DNA sensors …..………………………………... 118<br />
5.9.1 Electrochemical impedance spectroscopy ……………………………………… 118<br />
5.9.2 Cyclic voltammetry ……………………………………………………………… 118<br />
5.9.3 Chronocoulometry for determination of DNA coverage …………………………… 118<br />
5.10 Hybridization and dehybridization ..……………………………………… 118<br />
5.10.1 Detection of hybridization ………………………………………………………… 119<br />
5.10.2 DNA coverage determination by means of FSCV ...…………………....………… 119<br />
5.11 Intercalation .…………………………………………………………………… 120<br />
5.12 Methods …..……………………………………………………………………… 120<br />
5.12.1 Electrochemical impedance spectroscopy ………………………………………… 120<br />
5.12.2 Chronocoulometry for the determination of DNA coverage ...…………………… 124<br />
6. References ...………………………………………………………………….... 127<br />
7. Appendix ..……………………………………………………………………… 133<br />
7.1 List of symbols and abbreviations …...……………………………………… 133<br />
7.2 Publications list …..……………………………………………………………… 136<br />
7.3 Conference contributions ...…………………………………………………… 138