DISSERTATION
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_____________________________________________________________ Results and Discussion<br />
preparation. Therefore, the proposed method may become a new standard DNA immobilization<br />
procedure to obtain desired surface coverages in a very short time as a prerequisite for the<br />
development of highly sensitive and reproducible DNA hybridization assays with<br />
electrochemical read-out.<br />
Figure 3.30. Reproducibility of the potential-assisted immobilization method. Data<br />
obtained from EIS measurements for ssDNA/MCH-modified electrodes by fitting Nyquist<br />
plots to a [R(Q[RW])] equivalent circuit. The pulse profile for DNA immobilization was<br />
performed for 15 min using a 10 ms pulse duration. The black dashed line represents the<br />
average fitted Rct value for the MCH-modified electrode. Error bars represent the<br />
standard deviation between measurements (n > 3). Figure adapted from ref. 5 .<br />
3.3 Importance of controlling the surface 66