DISSERTATION

______________________________________________________________________ Introduction
detection can be performed using a redox mediator in solution. An alternative approach is to
hybridize a signal DNA with an overhang from the target DNA that is placed close to the
surface 65 . This way there is no need for the redox mediator to obtain the signal.
Figure 1.15. Indirect DNA detection using a) electrochemical mediators, b) non-covalently
interacting indicators, c-d) labelling of probe or target DNA and e) sandwich type
detection.
1.4 Hybridization detection 25