DISSERTATION

________________________________________________________________ Experimental Work
5.1 Materials and consumables
The following materials were bought from Sigma-Aldrich Chemie (Germany): 6-mercapto-1-
hexanol, 11-mercapto-1-undecanol, [Ru(NH3)6]Cl3, K3[Fe(CN)6] and K4[Fe(CN)6]. 16-
mercapto-1-hexadecanol was from Frontiers Scientific (United States). KH2PO4, K2HPO4 and
K2SO4 were from VWR International (Germany). Glucose was purchased from AppliChem
(Germany) and was used one day after the preparation of the solution to allow for mutarotation.
All reagents were of analytical grade and used as received. Polishing cloths were bought from
Leco (USA) including polishing pastes with 3 µm, 1 µm and 0.5 µm particle size. 0.1 µm
particle size polishing paste was from Struers (Germany). Non-modified (bare gold) 32-
electrode chips were bought from FRIZ Biochem (Germany).
Glucose oxidase from Aspergillus niger (type X-S, lyophilized powder, 100000–250000 U/g)
was purchased from Sigma-Aldrich and stored at -22 °C. All DNA probes were purchased from
FRIZ Biochem (Germany) and are listed in Table 5.1.
Table 5.1. Summary of DNA sequences used in all experiments. Sequences marked with –
SS were used as probe DNA, the rest of sequences were employed as target DNA. DTPA
stands for dithiophosphoramidite. Fc stands for ferrocene. A – adenine, T – thymine, C –
cytosine, G – guanine.
Internal name
Sequence
FRIZ 12-SS
5’ TGC GGA TAA CAC AGT CAC CT TTTTTTT (DTPA)3
FRIZ 5 tDNA
5’ AGG TGA CTG TGT TAT CCG CA
FRIZ 5-Fc
5’ (Fc)4 AGG TGA CTG TGT TAT CCG CA
E. coli-SS
E. coli-Fc
5’ GTC AAT GAG CAA AGG TAT TAA CTT TAC TCC
CTT CCT CCA TTA TTTTTTT (DTPA)3
5’ (Fc)4 GGA GGA AGG GAG TAA AGT TAA TAC CTT
TGC TCA TTG CG
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