DISSERTATION

_____________________________________________________________ Results and Discussion
usually occurs over a period of two days, the ability to reproducibly prepare DNA sensors
within minutes presents a significant improvement.
Figure 3.48. Dependence of dsDNA coverage on the total duration of ssDNA immobilization.
After performing ssDNA immobilization (0.5/-0.2 V vs. Ag/AgCl/3 M KCl pulse
profile, 10 ms pulse duration, 10 mM PB with 450 mM K2SO4 and 1 µM DNA) for a
predefined time, all electrodes were passivated with MCU (1 min, 0.5/-0.2 V vs. Ag/AgCl/3
M KCl pulse profile, 10 ms pulse duration, 10 mM PB with 20 mM K2SO4 and 1 mM
MCU) and subjected to hybridization with Fc-tDNA (10 min incubation, 37 °C, 10 mM
PB, 450 mM K2SO4, 1 µM DNA). FSCV:10 mM PB, 450 mM K2SO4, 1 V/s. Coverage
determination as described in Section 5.9.3.
3.4 Potential-assisted preparation of DNA sensors 90