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Malolactic Fermentation<br />

PROTOCOL FOR MALOLACTIC FERMENTATION IN DIFFICULT CONDITION<br />

In order to get successful malolactic fermentation in<br />

difficult wines (pH lower than 3.2, alcohol content<br />

> 14%) or to speed up the fermentation in normal<br />

conditions, we suggest to prepare a pied de cuve as<br />

following.<br />

EXAMPLE FOR 25 hL WINE<br />

1. Rehydrate 1 dose for 25 hL of Enartis ML Silver in<br />

0.5 litre of still mineral water at 20-25°C (68-77°F).<br />

2. Wait 15 minutes<br />

3. Dissolve 50 g of Nutriferm OsmoBacti directly in<br />

same water suspension and stir.<br />

4. Wait 2-4 hours (please note: follow the<br />

recommended timing). Keep the water suspension<br />

at 18-20°C (64-68°F)<br />

5. Prepare a blend with<br />

25 L water<br />

25 L wine<br />

500 g Nutriferm ML<br />

6. Adjust the blend pH > 3.3 if necessary.<br />

7. Inoculate the blend with the bacteria/OsmoBacti<br />

suspension. Keep it at 18-20°C (64,68°F).<br />

8. At 1/2 - 2/3 malic acid depletion, add 1 hL of wine<br />

into the pied de cuve in fermentation. Keep it at<br />

18-20°C (64-68°F).<br />

9. When 1/2 - 2/3 of malic acid content of the pied<br />

de cuve is consumed, use the pied de cuve to<br />

inoculate the remaining wine.<br />

10. Keep the wine at 18-20°C (64-68°F) till the end of<br />

the fermentation.<br />

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