Supplemental Figure 1 online. RT-PCR to Confirm ... - The Plant Cell

Supplemental Table 2 online. Mean Ct Values and LSDs of Gene Expression Studies Shown in
Figure 4A, B (GA-responsive genes) and of GA2ox3 and GA2ox6.
Sample At2g45210 At2g21220 At1g23205 SCL3 At5g45460
Wild type 25.8 ± 0.1 24.9 ± 0.0 22.1 ± 0.1 23.6 ± 0.0 25.7 ± 0.0
Wild type +GA 24.5 ± 0.0 ** 23.1 ± 0.1 ** 21.6 ± 0.1 ** 24.6 ± 0.0 ** 26.0 ± 0.0 **
ga2ox quintuple 25.3 ± 0.1 ** 24.3 ± 0.1 ** 21.5 ± 0.1 ** 24.1 ± 0.0 ** 26.0 ± 0.0 **
ga2ox quintuple +GA 24.5 ± 0.1 ** 23.0 ± 0.1 ** 21.7 ± 0.0 * 24.6 ± 0.1 ** 26.1 ± 0.1 **
LSD(5%/1%) (df) 0.34/0.50 (8) 0.29/0.42 (8) 0.26/0.38 (8) 0.11/0.16 (8) 0.15/0.21 (8)
n 3 3 3 3 3
Sample GA20ox1 GA3ox1 GA2ox8 GID1b
Wild type 24.9 ± 0.1 24.8 ± 0.1 28.9 ± 0.1 26.2 ± 0.0
Wild type +GA 25.6 ± 0.1 ** 25.6 ± 0.1 ** 27.4 ± 0.1 ** 27.4 ± 0.1 **
ga2ox quintuple 25.4 ± 0.0 ** 25.3 ± 0.1 * 28.2 ± 0.0 ** 26.5 ± 0.2
ga2ox quintuple +GA 25.8 ± 0.1 ** 25.8 ± 0.1 ** 27.3 ± 0.1 ** 27.4 ± 0.2 **
LSD(5%/1%) (df) 0.34/0.50 (8) 0.33/0.49 (8) 0.27/0.40 (8) 0.47/0.69 (8)
n 3 3 3 3
Sample GA2ox3 GA2ox6
Wild type 31.4 ± 0.4 27.4 ± 0.1
Wild type +GA 31.4 ± 0.1 28.0 ± 0.1
ga2ox quintuple - -
ga2ox quintuple +GA - -
LSD(5%/1%) (df) n.a. n.a.
n 3 3
See Methods for details on statistical analysis. The measurements are the mean reference-genes-
corrected threshold-cycle (Ct) values ± SE. *, significantly different from the untreated wild type
(P