Supplemental Figure 1 online. RT-PCR to Confirm ... - The Plant Cell

JL-202
CATTTTATAATAACGCTGCGGACA
TACTAC
GABI_LBo8409 ATATTGACCATCATACTCATTGC
FLAG_LB4
SLAT 3' dspm1
CGTGTGCCAGGTGCCCACGGAATA
GT
CTTATTTCAGTAAGAGTGTGGGGT
TTTGG
RT-PCR TESTING NULL STATUS OF INSERTION ALLELES
Primer Sequence Used for
GA2ox1 KOF TGACCAAAACACGGACTCGAT
GA2ox1 KOR CGGAGCGATTCTCTGAGTCAA
GA2ox2 KOF GAGAAGATGGTGAAGGTGGGG
GA2ox2 KOR AGCCAATCATCTTGCTCAGGG
GA2ox3 KOF1 GGAAGAGACTCCGGTCAAGGA
GA2ox3 KOR1 GCAATCATCTTGCTTTGGCAC
GA2ox3 KOF2 ACCGACTCAGATGCCAAAACC
GA2ox3 KOF2 TTTGCTCGACATTCTCTTCATCTC
allele, with GA2ox3 gR1:
850bp), ga2ox4-1 (mutant
allele, with GA2ox4 gR1:
1100bp), ga2ox4-2 (mutant
allele, with GA2ox4 gF1:
650bp)
Genotyping ga2ox2-3 (mutant
allele, with GA2ox2 gR:
1200bp), ga2ox4-4 (mutant
allele, with GA2ox4 gF2:
350bp), ga2ox6-3 (mutant
allele, with GA2ox6 gF1:
750bp)
Genotyping ga2ox4-3 (mutant
allele, with GA2ox4 gF1:
300bp)
Genotyping ga2ox3-2 (mutant
allele, with GA2ox3 gR2:
550bp),
Genotyping ga2ox6-2 (mutant
allele, with GA2ox6 gR1:
400bp)
RT-PCR of ga2ox1 mutants.
Wild type allele 358 bp.
RT-PCR of ga2ox2 mutants.
Wild type allele 320 bp.
RT-PCR of ga2ox3-1 mutant.
Wild type allele 328 bp.
RT-PCR of ga2ox3-2 mutant.
Wild type allele 345 bp.
GA2ox4 KOF TTCGCTCTCAGCTCTCCTTCA RT-PCR of ga2ox4 mutants.