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The premier international conference on laboratory aut<strong>omation</strong><br />
Final Program & Abstracts<br />
Your resource guide to:<br />
• Emerging and Enabling Technologies<br />
• World-Renowned Speakers and Experts<br />
• Groundbreaking Sessions on Clinical Advances<br />
• Largest Laboratory Aut<strong>omation</strong> Exhibition<br />
February 1 – 5, 2004 • San Jose McEnery Convention Center • San Jose, California<br />
Short Courses: Sunday and Monday, February 1 and 2, 2004<br />
Exhibition Opening: Monday evening, February 2, 2004<br />
Sessions: Tuesday, Wednesday, and Thursday, February 3, 4 and 5, 2004<br />
ALA_office@labaut<strong>omation</strong>.org<br />
labaut<strong>omation</strong>.org<br />
program final program final program final program final program final program final program final program final program
The premier international conference on laboratory aut<strong>omation</strong><br />
TABLE OF CONTENTS<br />
Sponsors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2<br />
Welcome . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3<br />
Conference Chairs & Scientific Committee . . . . . . . . . . . . . . . . . . . . . . 4<br />
Board of Directors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5<br />
ALA Committees . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6<br />
ALA Me<strong>mbers</strong>hip Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7<br />
General Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8<br />
Tribute to Dave Herold & Tony Beugelsdijk . . . . . . . . . . . . . . . . . . . . . 10<br />
Plenary Program Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12<br />
Conference Floor Plan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13<br />
Conference-at-a-Glance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14<br />
Poster Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25<br />
Podium Abstracts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41<br />
Poster Abstracts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 145<br />
Industry Sponsored Workshop Luncheons . . . . . . . . . . . . . . . . . . . . 237<br />
Exhibit Hall Floorplan and Exhibitor listing . . . . . . . . . . . . . . . . . . . . 246<br />
Short Courses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 297<br />
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 305<br />
ALA_office@labaut<strong>omation</strong>.org<br />
labaut<strong>omation</strong>.org
UNRESTRICTED SPONSORS<br />
Unrestricted sponsors allow ALA to attract leading scientists to reveal and discuss emerging<br />
technologies from the podium. These funds are also used to assist with the travel and<br />
registration of students. ALA thanks the following companies for their support:<br />
FRIENDS OF ALA<br />
ALA encourages special interest groups, associations,<br />
and other organizations focused on education and<br />
improving the practice of laboratory aut<strong>omation</strong> to<br />
gather during LabAut<strong>omation</strong>2004. With the Friends<br />
program, organizations, special interest groups<br />
and “Birds of a Feather” consortia have the unique<br />
opportunity to come together to meet, share common<br />
experiences, and further enhance their educational<br />
experiences. ALA welcomes the following Friends to<br />
LabAut<strong>omation</strong>2004:<br />
SPONSORSHIPS<br />
2<br />
EXCHANGE PARTNERS<br />
ALA thanks the following companies for their support<br />
in creating awareness of LabAut<strong>omation</strong>2004 through<br />
mutually beneficial sponsorship exchange agreements:<br />
MicroTAS
February 1-5, 2004<br />
San Jose McEnery<br />
Convention Center<br />
San Jose, California<br />
WELCOME TO LABAUTOMATION2004!<br />
The Association for Laboratory Aut<strong>omation</strong> (ALA)<br />
welcomes you to San Jose and LabAut<strong>omation</strong>2004, the<br />
premier conference and exhibition on emerging laboratory<br />
technologies! For eight years, LabAut<strong>omation</strong> has driven<br />
ground-breaking advances in the pharmaceutical, clinical,<br />
and biotechnology fields through cutting-edge laboratory<br />
technology, education, and collaboration. We’re thrilled<br />
that you’re a part of this pivotal event!<br />
Over the next few days, you will gain valuable knowledge<br />
and resources for leveraging new discoveries and<br />
tools. You’ll meet international colleagues, cultivate new<br />
relationships, and find innovative ways to collaborate<br />
in the laboratory environment. You’ll also enjoy an<br />
educational program with engaging sessions, intriguing<br />
speakers, and upbeat networking events – thanks to<br />
LabAut<strong>omation</strong>2004 Scientific Committee me<strong>mbers</strong>, who<br />
worked nonstop to bring it all together!<br />
This year’s program is the best yet! In addition to<br />
dynamic short courses and provocative educational<br />
sessions, LabAut<strong>omation</strong>2004 features an expansive<br />
exhibition floor with more than 300 booths. So be<br />
sure to visit the exhibit hall throughout the week! You’ll<br />
see firsthand the latest in aut<strong>omation</strong> technologies for<br />
pharmaceutical, biotechnology, clinical, and chemical<br />
laboratories – from companies around the world.<br />
Short Courses: Sunday and Monday, February 1 and 2, 2004<br />
Exhibition Opening: Monday evening, February 2, 2004<br />
Sessions: Tuesday, Wednesday, and Thursday, February 3, 4 and 5, 2004<br />
Highlights of LabAut<strong>omation</strong>2004 include:<br />
• 21 One-Day and Two-Day Short Courses<br />
• Plenary Session Speakers: Dr. Jurgen Drews,<br />
Mr. John D. Rhodes, Dr. Chris Lowe, and<br />
Dr. Richard Mathies<br />
• Seven Educational Tracks: High Throughput<br />
Chemistry, High Throughput Screening, Micro-<br />
Technologies, Proteomics, Genomics, Clinical, and<br />
Emerging Technologies<br />
• 192 Podium Presentations<br />
• Hundreds of Poster Presentations<br />
• ALA’s Annual GALA Dinner<br />
• Jouan Robotics Award<br />
ALA continues to be the only member-driven, nonprofit<br />
organization dedicated to advancing the science<br />
and interests of laboratory aut<strong>omation</strong> professionals<br />
everywhere – so that you can make great things<br />
happen every day.<br />
We’re confident that LabAut<strong>omation</strong>2004 will give you<br />
fresh perspectives, knowledge, and enthusiasm for<br />
the future of laboratory aut<strong>omation</strong>. Please use this<br />
program as a resource guide in the days ahead to help<br />
you make the most of this great opportunity. Enjoy your<br />
visit to San Jose!<br />
3<br />
Sincerely,<br />
The LabAut<strong>omation</strong>2004 Scientific Committee<br />
ALA_offi ce@labaut<strong>omation</strong>.org<br />
labaut<strong>omation</strong>.org<br />
SPONSORSHIPS | WELCOME
CONFERENCE CHAIRS<br />
David Herold, M.D., Ph.D.<br />
Program Chairman<br />
VA San Diego Healthcare System/<br />
University of California, San Diego<br />
SCIENTIFIC COMMITTEE (BY TRACK)<br />
Analytical<br />
Michael Greig, Chair<br />
Pfizer Global R&D – La Jolla<br />
Steven A. Hofstadler, Ph.D., Associate Chair<br />
Ibis Therapeutics, Inc.<br />
Clinical<br />
Charles Hawker, Ph.D., Chair<br />
ARUP Laboratories<br />
William Neeley, M.D., Associate Chair<br />
Detroit Medical Center<br />
Emerging Technologies<br />
W. Steve Fillers, Ph.D., Chair<br />
TekCel, Inc.<br />
Stewart Chipman, Ph.D., Associate Chair<br />
SDC Associates, Inc.<br />
Genomics<br />
Jose Olivares, Ph.D., Chair<br />
Los Alamos National Laboratory<br />
Scott Hunicke-Smith, Ph.D., Associate Chair<br />
Ambion, Inc.<br />
High Throughput Chemistry<br />
Nicholas Hird, Ph.D., Chair<br />
Takeda Chemical Industries, Ltd.<br />
Michal Lebl, Ph.D., Associate Chair<br />
Illumina, Inc.<br />
High Throughput Screening<br />
Richard Nelson, Ph.D., Chair<br />
Boehringer Ingelheim Pharmaceuticals, Inc.<br />
Tina Garyantes, Ph.D., Associate Chair<br />
Aventis, Inc.<br />
4<br />
Andrew Zaayenga<br />
Associate Program Chairman<br />
TekCel, Inc.<br />
Informatics<br />
Jay Gill, Ph.D., Chair<br />
Bristol-Myers Squibb Co.<br />
Carl Murray, Ph.D., Associate Chair<br />
Beckman Coulter, Inc.<br />
Micro-Technologies<br />
Anne Kopf-Sill, Ph.D., Chair<br />
Caliper Technologies Corporation<br />
Laurie Locascio, Ph.D., Associate Chair<br />
National Institute of Standards and Technology<br />
Posters<br />
Gary W. Kramer, Ph.D., Chair<br />
National Institute of Standards and Technology<br />
William Sonnefeld, Ph.D., Associate Chair<br />
Eastman Kodak Company<br />
Proteomics<br />
Robert Fitzgerald, Ph.D., Chair<br />
VA San Diego Healthcare System/<br />
University of California, San Diego<br />
Eric Peters, Ph.D., Associate Chair<br />
Novartis Research Foundation<br />
Short Courses<br />
Steven D. Hamilton, Ph.D., Coordinator of Software<br />
Short Courses<br />
Sanitas Consulting<br />
Mark F. Russo, Ph.D., Coordinator of Software Short Courses<br />
Bristol-Myers Squibb Co.
ALA BOARD OF DIRECTORS<br />
Peter Grandsard, Ph.D.<br />
Amgen, Inc.<br />
Steven D. Hamilton, Ph.D.<br />
Sanitas Consulting<br />
E. Kevin Hrusovsky, M.B.A.<br />
Caliper Technologies<br />
Mark F. Russo, Ph.D.<br />
Bristol-Myers Squibb Co.<br />
Torsten A. Staab, MS,<br />
Dipl. Inform. (FH)<br />
Los Alamos National Laboratory<br />
Andy Zaayenga<br />
TekCel, Inc.<br />
5<br />
ALA Welcomes New<br />
Elected Board Me<strong>mbers</strong><br />
The results from ALA’s member election<br />
are finally tallied. This year’s election<br />
once again proved ALA is truly a<br />
member-driven, volunteer organization<br />
with 14 candidates vying for three Board<br />
openings. The ALA welcomes Anne<br />
Kopf-Sill, James Myslik, and Stephen<br />
Jacobson (re-elected) to their new roles<br />
on the Board of Directors. Their terms<br />
commence immediately and carry for<br />
the next three years. Please be sure to<br />
congratulate Anne, James and Stephen.<br />
We are proud they are involved with the<br />
ALA leadership team.<br />
Anne Kopf-Sill, Ph.D.<br />
Caliper Technologies Corporation<br />
James Myslik, Ph.D.<br />
Bristol-Myers Squibb Co.<br />
Stephen C. Jacobson, Ph.D.<br />
Indiana University (re-elected)<br />
ALA’s Professional Team …be sure to visit the ALA Team at Booth 1607<br />
Greg Dummer, CAE<br />
Chief Administrative Offi cer<br />
Joann Bellos<br />
Account Reconciliation<br />
Leona Caffey<br />
Production, Copy Coordination<br />
Brian Casey, CEM<br />
Director, Event Management<br />
Brenda Dreier<br />
Abstract & Speaker<br />
Management<br />
Jeanne Farrell<br />
Marketing Specialist<br />
Peter Gaido, Esq.<br />
Gaido & Fintzen<br />
Legal Counsel<br />
Daphne Glover<br />
Exhibit Sales & Sponsorships<br />
Nan Hallock<br />
Managing Editor<br />
Journal of the Association for<br />
Laboratory Aut<strong>omation</strong> (JALA)<br />
Keri Hinton<br />
Housing & Registration<br />
David Laurenzo<br />
Director, Marketing &<br />
Communications<br />
Jim Laurenzo<br />
Marketing Operations Specialist<br />
Kathy Maher<br />
Accounts Payable<br />
Karen Miller<br />
Accounts Receivable<br />
Koranne Ostic<br />
Web Designer<br />
Michael Randow<br />
Web Manager<br />
Sarah Rhees<br />
Conference Manager<br />
Dave Wasielewski, CPA<br />
Director, Financial Management<br />
Francine Ziev<br />
Designer<br />
SCIENTIFIC COMMITTEE & BOARD OF DIRECTORS
ALA COMMITTEES<br />
Audit Committee<br />
E. Kevin Hrusovsky, M.B.A.,<br />
Chairman<br />
Caliper Technologies<br />
Corporation<br />
Jim Bruner<br />
GlaxoSmithKline<br />
Sheila DeWitt, Ph.D.<br />
Arqule<br />
Jay Gill, Ph.D.<br />
Bristol-Myers Squibb Co.<br />
Ann Janssen<br />
Pfizer<br />
Bylaws Committee<br />
Torsten A. Staab, MS, Dipl.<br />
Inform. (FH), Chairman<br />
Los Alamos National Laboratory<br />
John Elling, Ph.D., M.B.A.<br />
Datect, Inc.<br />
Doug Gurevitch, M.S., P.E.<br />
University of California, San<br />
Diego<br />
Paul Rodziewicz<br />
ReTiSoft, Inc.<br />
Reinhold Schäfer, Ph.D.<br />
Fachhochschule Wiesbaden<br />
Education Committee<br />
Jim Sterling, Ph.D., Chairman<br />
Keck Institute<br />
Peter Grandsard, Ph.D.<br />
Amgen, Inc.<br />
Steven Hamilton, Ph.D.<br />
Sanitas Consulting<br />
Mark Russo, Ph.D.<br />
Bristol-Myers Squibb Co.<br />
Sabeth Verpoorte, Ph.D.,<br />
University of Groningen<br />
Andy Zaayenga<br />
TekCel, Inc.<br />
Finance Committee<br />
Steven Hamilton, Ph.D.,<br />
Chairman<br />
Sanitas Consulting<br />
Richard Nelson, Ph.D.<br />
Boehringer-Ingelheim<br />
William Sonnefeld, Ph.D.<br />
Eastman Kodak<br />
Jouan Committee<br />
David A. Herold, M.D., Ph.D.,<br />
Chairman<br />
VASDHS/UCSD<br />
Tina Garyantes, Ph.D.<br />
Aventis<br />
Jay Gill, Ph.D.<br />
Bristol-Myers Squibb Co.<br />
Scott Hunicke-Smith, Ph.D.<br />
Ambion, Inc.<br />
Michal Lebl, Ph.D.<br />
Illumina, Inc.<br />
Laurie Locascio, Ph.D.<br />
National Institute of Standards<br />
and Technology<br />
Elaine Mardis, Ph.D.<br />
Washington University School<br />
of Medicine<br />
Eric Peters, Ph.D.<br />
Novartis Research Foundation<br />
Alain Truchaud, Ph.D.<br />
Institut de Biologie<br />
Juergen Zimmerman, Ph.D.<br />
EMBL<br />
Me<strong>mbers</strong>hip Committee<br />
Andy Zaayenga, Chairman<br />
TekCel, Inc.<br />
Nicholas Hird, Ph.D.<br />
Takeda Chemical Industries, Ltd.<br />
Karen Kearns, M.B.A., B.S.<br />
Amgen, Inc.<br />
Brian Lightbody<br />
Caliper Technologies<br />
Corporation<br />
Erik Rubin, Ph.D.<br />
Bristol-Myers Squibb Co.<br />
Reinhold Schäfer, Ph.D.<br />
Fachhochschule Wiesbaden<br />
Torsten A. Staab, MS, Dipl.<br />
Inform. (FH)<br />
Los Alamos National Laboratory<br />
Kailash Swarna,<br />
Novartis Institutes for<br />
BioMedical Research, Inc.<br />
6<br />
Nominating Committee<br />
Tony J. Beugelsdijk, Ph.D.,<br />
M.B.A., Chairman<br />
Los Alamos National Laboratory<br />
Jay Gill, Ph.D.<br />
Bristol-Myers Squibb Co.<br />
David A. Herold, M.D., Ph.D.<br />
VASDHS/UCSD<br />
Michal Lebl, Ph.D.<br />
Illumina, Inc.<br />
Kerstin Thurow, Ph.D.<br />
University of Rostock<br />
JALA Editorial Board<br />
Mark Russo, Ph.D., Chairman<br />
Bristol-Myers Squibb Co.<br />
Thomas W. Astle, P.E.<br />
Mark Beggs, Ph.D.<br />
Tony J. Beugeldijk. Ph.D.,<br />
M.B.A.<br />
Raymond Dessy, Ph.D., D.Sc.<br />
Peter Grandsard, Ph.D.<br />
Steven D. Hamilton, Ph.D.<br />
C. John Harris, Ph.D.,<br />
Cchem, FRSC<br />
David A. Herold, M.D., Ph.D.<br />
Sue Holland, B.Sc., Ph.D.<br />
Leroy Hood, M.D., Ph.D.<br />
Paul S. Kayne, Ph.D.<br />
Anne R. Kopf-Sill, Ph.D.<br />
Gary W. Kramer, Ph.D.<br />
Rodney Markin, M.D., Ph.D.<br />
Ben Moshiri, Ph.D.<br />
Carl Murray, Ph.D.<br />
Giles Sanders, Ph.D.<br />
Reinhold Schaefer, Ph.D.<br />
John H.M. Souverijn, Ph.D.<br />
Torsten A. Staab, MS, Dipl.<br />
Inform. (FH)<br />
Tetsuro Sugiura, M.D., Ph.D.<br />
Pieter Telleman, Ph.D.<br />
Kerstin Thurow, Ph.D.<br />
Alain Truchaud, Ph.D.<br />
Sabeth Verpoorte, Ph.D.<br />
Hilmar Weinmann, Ph.D.<br />
Mike Wheeler, Ph.D.<br />
Ad-Hoc Exhibitor Advisory<br />
Committee<br />
Jay Smith, Chairman<br />
deCODE genetics<br />
Tom Astle, P.E.<br />
TomTec<br />
Anna Barcelos<br />
TekCel, Inc.<br />
Marc Boillat<br />
Seyonic<br />
Dennis Claspell<br />
Gilson<br />
Carol Huggins<br />
Beckman-Coulter, Inc.<br />
Elizabeth Savelle<br />
Velocity 11<br />
Peter Siesel<br />
Tecan<br />
Lynda Thomas<br />
Thermo Electron<br />
ALA LabFusion 2004<br />
Scientific Committee<br />
Tony J. Beugelsdijk, Ph.D.,<br />
M.B.A., Chairman<br />
Los Alamos National Laboratory<br />
Peter Gransard, Ph.D.,<br />
Associate Chairman<br />
Amgen, Inc.<br />
Carmen Baldino, Ph.D.<br />
Arqule<br />
Andrea Chow, Ph.D.<br />
Caliper Corporation<br />
Rod Cole, Ph.D.<br />
Millenium Pharmaceuticals<br />
Carol Homon, Ph.D.<br />
Boehringer Ingelheim<br />
Brian Lightbody<br />
Caliper Technologies<br />
Corporation<br />
Tom Olah, Ph.D.<br />
Bristol-Myers Squibb Co.<br />
Robert Pacifici, Ph.D.<br />
Eli Lilly<br />
Scott Patterson, Ph.D.<br />
Amgen, Inc.<br />
John Stults, Ph.D.<br />
BioSpect<br />
Sabeth Verpoorte, Ph.D.<br />
University of Groningen<br />
Harold Weller, Ph.D.<br />
Bristol-Myers Squibb Co.
Great Things Happen Every Day When You Discover ALA<br />
The Association for Laboratory Aut<strong>omation</strong> (ALA) is making an exciting difference in the field of laboratory<br />
aut<strong>omation</strong>. We are the only member-driven, non-profit organization dedicated to advancing the interests of<br />
laboratory aut<strong>omation</strong> professionals everywhere. By joining ALA or renewing your me<strong>mbers</strong>hip today, you’re taking<br />
a quantum leap forward for your career and the fast-evolving field of laboratory aut<strong>omation</strong>.<br />
Enjoy the Rewards of ALA Me<strong>mbers</strong>hip<br />
ALA me<strong>mbers</strong>hip offers an array of rich benefits, including:<br />
• Discounts on our premier educational conferences:<br />
LabAut<strong>omation</strong>2005 – Focuses on discovery, tools, and emerging technologies.<br />
ALA LabFusion 2004 – Focuses on development and applications,<br />
with an emphasis on biological and pharmaceutical interests.<br />
• Subscription to the Journal of the Association for Laboratory Aut<strong>omation</strong> (JALA), a multi-disciplinary<br />
international forum devoted to the advancement of technology in the laboratory.<br />
• Opportunities to network with world-renowned experts and cutting-edge organizations in the field of laboratory<br />
science through ALA events and forums.<br />
• Access to a collaborative, synergistic community, where professionals come together to share common<br />
concerns, ideas, and interests.<br />
• Professional development through volunteer leadership opportunities.<br />
• Annual me<strong>mbers</strong>hip directory with online access.<br />
Please visit labaut<strong>omation</strong>.org/me<strong>mbers</strong>hip to join ALA or renew your me<strong>mbers</strong>hip today!<br />
Make A Difference Today With ALA<br />
We are committed to accelerating progress in the global scientific<br />
community, propelling the field of laboratory aut<strong>omation</strong> and your<br />
career to new frontiers. Part of what makes this possible is our<br />
diverse team of committed ALA volunteers, who bring their unique<br />
perspectives and expertise to the organization and the field of<br />
laboratory aut<strong>omation</strong>.<br />
Expand your education as a valued ALA volunteer – collaborate<br />
on ALA program content, shape ALA’s strategy and vision for<br />
the future, create sound policies and procedures, and even lead<br />
special committees. By getting involved, you will have unparalleled<br />
opportunities to grow personally and professionally – and make a real<br />
difference in the field of laboratory aut<strong>omation</strong>.<br />
We hope you will join our growing list of volunteers by calling (866) 263-4928 today!<br />
JALA<br />
As a member of ALA, you will receive the Journal of the Association for Laboratory Aut<strong>omation</strong> (JALA), a reviewed<br />
scientific journal providing constructive articles on commercially available and new technology shaping the future.<br />
If you are interested in submitting a manuscript, contact JALA Managing Editor, Nan Hallock, at (920) 652-0427;<br />
nanhallock@labaut<strong>omation</strong>.org.<br />
Become an ALA member!<br />
7<br />
“At the core of our organization<br />
is our volunteer base – individuals<br />
whose vision and dedication is<br />
our greatest asset.”<br />
Join ALA now for a chance to win a palmOne Zire Handheld.<br />
Visit us in Booth 1607 by February 3 to enter.<br />
– Tony Beugelsdijk, Ph.D., M.B.A.<br />
Los Alamos National Laboratory<br />
Los Alamos New Mexico<br />
ALA COMMITTEES | ALA MEMBERSHIP INFORMATION
GENERAL INFORMATION<br />
CONFERENCE LOCATION<br />
The exhibition, podium sessions, posters,<br />
registration, and internet access are located in<br />
the San Jose McEnery Convention Center.<br />
CONFERENCE BADGES<br />
Those entering the Exhibition must be wearing<br />
an official conference name badge or an<br />
exhibit-only name badge.<br />
ACCOMMODATIONS<br />
Most attendees are housed in the hotels listed<br />
below.<br />
Fairmont Hotel (408) 998-1900<br />
Crowne Plaza (408) 998-0400<br />
Marriott Hotel (408) 280-1300<br />
Hilton Hotel (408) 287-2100<br />
PODIUM SESSIONS<br />
Speakers are asked to appear 30 minutes prior to the<br />
start of their sessions. If using the LCD data projector,<br />
speakers must provide a laptop. There is also an<br />
overhead projector.<br />
SPEAKER READY<br />
Speakers may preview their presentations on the<br />
LCD projectors located in Room D of the Convention<br />
Center. This room is open to Short Course instructors<br />
on Sunday from 7:30 am – 5:00 pm and Monday from<br />
7:30 am – 7:00 pm. It will be open to all other<br />
instructors on Monday from 5:00 – 7:00 pm and<br />
Tuesday, Wednesday, and Thursday from 7:30 am –<br />
5:00 pm.<br />
POSTERS<br />
There are two poster sessions, one on Tuesday and<br />
the second on Wednesday. Posters should be set up<br />
10:00 – 10:30 am on the designated day and removed<br />
by 6:30 pm on the designated day.<br />
• Tuesday Posters. Presenters should attend<br />
their posters 1:30 – 3:00 pm on Tuesday.<br />
Tuesday posters are annotated TP.<br />
• Wednesday Posters. Presenters should attend<br />
their posters 2:00 – 3:30 pm on Wednesday.<br />
Wednesday posters are annotated WP.<br />
8<br />
PRINTED PROGRAM AND ABSTRACTS<br />
The titles and abstracts printed in this program were<br />
entered on-line by the authors. It is not possible to fully<br />
edit this material. Information is subject to change.<br />
CHILD CARE<br />
Childcare service has been contracted with KiddieCorp,<br />
an independent company. Advance reservations are<br />
required. Childcare is located in Room K of the lower<br />
level.<br />
EXHIBITS<br />
Exhibits are open 4:00 – 8:00 pm on Monday, and<br />
10:00 am – 6:30 pm on Tuesday and Wednesday. The<br />
Association for Laboratory Aut<strong>omation</strong> extends sincere<br />
appreciation for the support of exhibitors.<br />
INTERNET ACCESS<br />
A limited number of workstations and laptop<br />
connections are available in Exhibit Hall 3. Your use will<br />
be limited to 20 minutes if others are waiting.
WORKSHOP LUNCHEONS/HOSPITALITY SUITES/PRESS CONFERENCES<br />
Guests for workshop luncheons should have pre-registered through the company web sites. If you have not<br />
registered, please visit the company’s exhibit booth to inquire. Evening hospitality suites are open to all.<br />
Day Time Location Event<br />
Tuesday 7:30 – 10:30 am Room J4 Nanostream Press Breakfast<br />
Tuesday 12:00 – 1:30 pm Marriott Hotel, Willow Glen Dahlgren Communications Press Lunch<br />
Tuesday 12:00 – 1:30 pm Rooms F Genetix Workshop Lunch<br />
Tuesday 12:00 – 1:30 pm Room A8 Greiner Bio-One Workshop Lunch<br />
Tuesday 12:00 – 1:30 pm Room C3 IDBS Workshop Lunch<br />
Tuesday 12:00 – 1:30 pm Room J4 Millipore Corporation Workshop Lunch<br />
Tuesday 12:00 – 1:30 pm Room N Tecan Workshop Lunch 1<br />
Tuesday 12:00 – 1:30 pm Room C2 Tecan Workshop Lunch 2<br />
Tuesday 12:00 – 1:30 pm Room J2 TekCel Workshop Lunch 1<br />
Tuesday 12:00 – 1:30 pm Room J1 The Aut<strong>omation</strong> Partnership Workshop Lunch<br />
Tuesday 12:00 – 1:30 pm Room J3 Thermo Electron Workshop Lunch<br />
Tuesday 2:00 – 4:00 pm Room J1 TekCel Press Conference<br />
Tuesday 8:00 – 9:00 pm Room J3 Bio-Tek Hospitality Suite<br />
Tuesday 8:00 – 9:00 pm Room J1 Nanostream Hospitality Suite<br />
Tuesday 8:00 – 9:00 pm Room J2 PerkinElmer Life Hospitality Suite<br />
Wednesday 12:30 – 2:00 pm Room J4 Beckman Coulter Workshop Lunch<br />
Wednesday 12:30 – 2:00 pm Room F Caliper/Zymark Workshop Lunch<br />
Wednesday 12:30 – 2:00 pm Room A8 JALA Prospective Authors Workshop<br />
Wednesday 12:30 – 2:00 pm Room J1 MDL Information Services Workshop Lunch<br />
Wednesday 12:30 – 2:00 pm Room C2 Tecan Workshop Lunch 3<br />
Wednesday 12:30 – 2:00 pm Room J2 TekCel Workshop Lunch 2<br />
Wednesday 12:30 – 2:00 pm Room J3 Thermo Electron Workshop Lunch<br />
REFRESHMENTS<br />
Breakfast<br />
A light continental breakfast will be served outside<br />
the Exhibit Hall Concourse, 7:30 – 8:00 am, Tuesday,<br />
Wednesday, and Thursday for full-registration<br />
attendees.<br />
Lunch<br />
Box lunches for full-registration and exhibits-only<br />
attendees will be served in the Exhibit Hall on Tuesday<br />
and Wednesday. A buffet lunch will be served in the<br />
Ballroom Concourse on Thursday.<br />
9<br />
Receptions<br />
There will be informal receptions in the exhibit hall as<br />
follows:<br />
Monday 4:00 – 8:00 pm<br />
Tuesday 5:00 – 6:30 pm<br />
Wednesday 5:30 – 6:30 pm<br />
GALA Dinner<br />
The GALA Dinner for full-registration attendees is<br />
Tuesday, February 3 at 7:00 pm in the Fairmont<br />
Hotel. Please present the ticket provided with your<br />
registration materials.<br />
SMOKING<br />
California law prohibits smoking in all public places,<br />
including the Convention Center and hotel lobbies.<br />
GENERAL INFORMATION
Association for Laboratory Aut<strong>omation</strong><br />
Thanks Founding Board Me<strong>mbers</strong><br />
Tony J. Beugelsdijk<br />
Ph.D., M.B.A.<br />
Los Alamos<br />
National Laboratory<br />
David A. Herold<br />
M.D., Ph.D.<br />
VA Healthcare System<br />
UC-San Diego<br />
ALA Co-Founders<br />
Board Me<strong>mbers</strong><br />
Committee Chairs and Volunteers: 1995-2004<br />
Enthusiastic and Informed Sources for<br />
Guidance and Advice: Forever
Tony Beugelsdijk and Dave Herold have taken a lot of things about ALA personally over<br />
the years…and we are thankful that they did. It was their personal commitment, faith, and<br />
sweat equity that made our association the practical and productive reality that it is today.<br />
As laboratory technology and aut<strong>omation</strong> began to take shape as a professional specialty in<br />
the 1980s, Tony and Dave were among a core group of mavericks that immediately recognized<br />
the need for a neutral organization to deliver information, education, and networking<br />
opportunities for like-minded experts. Early collaborations with Switzerland’s International<br />
Conference on Aut<strong>omation</strong> and Robotics eventually led to the U.S.-based Association for<br />
Laboratory Aut<strong>omation</strong>.<br />
Neither Tony nor Dave ever doubted that the ALA would be a success -- their personal<br />
determination and can-do attitudes guaranteed it, and the first ALA LabAut<strong>omation</strong><br />
Conference launched in San Diego in January 1997. As planned and predicted, the event<br />
and the organization achieved an important balance among a diversity of academic and<br />
commercial interests. ALA paired theory with practice, making it a popular and valuable<br />
resource for scientists and technologists from throughout the U.S. and around the world.<br />
In the process, Tony and Dave’s responsibilities were many: from sorting, packaging, and<br />
mailing 4,000 pounds of printed materials on the floor of Dave’s office; taste testing and<br />
selecting which California wines would be served; clearing their throats and making sales<br />
calls to hundreds of carefully researched and targeted exhibitor prospects; managing the<br />
schedules and requirements of hundreds of speakers and volunteers; to personally<br />
guaranteeing hundreds of thousands of dollars in hotel and convention center contract<br />
commitments. They worked nonstop, often through holidays, and often with help from<br />
their families, friends, and a growing network of interested and enthusiastic volunteers.<br />
As the organization and its events grew, Tony and Dave continued to keep a forward focus.<br />
By 2001, they recognized their original management model did not scale with ALA’s<br />
growth; it had become obsolete. By 2002, they had rectified the situation – an association<br />
management professional was hired to provide experienced leadership; bylaws, policies,<br />
and operating procedures were reconsidered and appropriately rewritten; and the organization<br />
moved from a self-appointed to a member-elected board of directors. It is this change<br />
in the board leadership structure that brings their tenure to an end.<br />
After nearly a decade of faith, hope, and dreams, Tony and Dave will retire from the ALA<br />
Board of Directors in February 2004. They take with them the countless memories,<br />
experiences, and new friends they earned over the years, and they leave behind a legacy<br />
of greater good. They look forward to being lifelong me<strong>mbers</strong> of ALA, participating in<br />
annual conferences, continuing to serve as me<strong>mbers</strong> of committees, and hoping that<br />
you’ll join them.<br />
On behalf of all me<strong>mbers</strong> of the Association for Laboratory Aut<strong>omation</strong>, we sincerely<br />
thank Dave and Tony for their leadership. As me<strong>mbers</strong> of the 2003 ALA Board of Directors,<br />
we look forward to continuing to build on their foundation of excellence…<br />
Peter Grandsard, Ph.D.<br />
Amgen, Inc.<br />
Torsten A. Staab, M.S.,<br />
Dipl. Inform. (FH)<br />
Los Alamos<br />
National Laboratory<br />
Steven D. Hamilton, Ph.D.<br />
Sanitas Consulting<br />
E. Kevin Hrusovsky<br />
Caliper Technologies Corp.<br />
Andrew Zaayenga<br />
TekCel, Inc.<br />
www.labaut<strong>omation</strong>.org<br />
Mark F. Russo, Ph.D.<br />
Bristol-Myers Squibb Co.<br />
Stephen C. Jacobson, Ph.D.<br />
Indiana University
PLENARY PROGRAM OVERVIEW<br />
Keynote Plenary Speaker<br />
Changing Patterns in the Discovery of New Drugs<br />
Tuesday, February 3, 2004 8:30 – 9:15 am<br />
Dr. Jürgen Drews<br />
Managing Partner<br />
Nigeons GmbH<br />
Financial Plenary Speaker<br />
Financial Forces Shaping the Future of the<br />
Pharmaceuticals and Biotech Industry<br />
Tuesday, February 3, 2004 9:15 – 10:00 am<br />
Mr. John D. Rhodes<br />
National Managing Partner, Life Sciences<br />
Deloitte & Touche<br />
Plenary Speaker<br />
Microfluidics in Your Future<br />
Tuesday, February 3, 2004 10:30 – 11:15 am<br />
Dr. Richard A. Mathies<br />
Professor of Biophysical and Bioanalytical Chemistry<br />
University of California, Berkeley<br />
Plenary Speaker<br />
The Nanophysiometer: Holographic Sensors for<br />
Drug Discovery<br />
Tuesday, February 3, 2004 11:15 am – 12:00 pm<br />
Dr. Christopher R. Lowe<br />
Professor of Biotechnology<br />
University of Cambridge<br />
12
CONFERENCE FLOOR PLAN<br />
San Jose McEnery Convention Center<br />
Exhibit Level<br />
E<br />
D<br />
Sessions<br />
C3<br />
Sessions<br />
C4<br />
C2 C1<br />
B3<br />
K<br />
(street level)<br />
B4<br />
B2 B1<br />
Registration Industry<br />
Workshops<br />
Escalators<br />
down to entrance<br />
A4<br />
N<br />
(street level)<br />
A5<br />
A3 A6<br />
A2 A7<br />
A1 A8<br />
Exhibit Hall<br />
and Posters<br />
13<br />
F<br />
J3<br />
J2<br />
J1 J4<br />
PLENARY PROGRAM OVERVIEW | CONFERENCE FLOOR PLAN
CONFERENCE AT A GLANCE<br />
8:30 am – 4:30 pm<br />
Please Note: The exhibit opening is on Monday evening and sessions commence on Tuesday.<br />
8:00 am – 5:00 pm Exhibitor Set Up<br />
8:30 am – 4:30 pm<br />
8:00 am – 2:00 pm Exhibitor Set Up<br />
Sunday, February 1, 2004<br />
Short Courses<br />
Barcode Technology • Economic Justification of Laboratory Aut<strong>omation</strong> • Emerging IT for the Laboratory<br />
Introduction to High Throughput Screening • Introduction to Laboratory Aut<strong>omation</strong> • Introduction to LabView<br />
Introduction to Molecular Biology • Mass Spectrometry Instrumentation & High Throughput Analysis<br />
Microarrays & Applications • Migrating from VB6 to VB.Net<br />
Sunday and Monday (two day courses)<br />
Getting Started With Excel and VBA in the Laboratory • Microfluidics I & II<br />
Monday, February 2, 2004<br />
Short Courses<br />
Biostatistics • Introduction to Databases in the Laboratory • Introduction to Laboratory Aut<strong>omation</strong><br />
LIMS in the Organization • Mass Spectrometry in Proteomics & Drug Discovery • Mathematica<br />
Molecular Diagnostic Aut<strong>omation</strong> • Patent Law • Pharmacogenomic Aut<strong>omation</strong><br />
Trajectory of Clinical Laboratory Aut<strong>omation</strong><br />
Sunday and Monday (two day courses)<br />
Getting Started With Excel and VBA in the Laboratory • Microfluidics I & II<br />
4:00 – 8:00 pm Opening Reception in the Exhibit Hall<br />
8:15 am – 12:00 pm Plenary Session, Ballroom A2<br />
10:00 am – 6:30 pm Exhibits Open<br />
12:00 – 1:30 pm Lunch for Attendees in the Exhibit Hall<br />
1:30 – 3:00 pm Poster Session in the Exhibit Hall<br />
3:00 – 5:00 pm<br />
HT Chemistry<br />
Room B3<br />
HTS Ion<br />
Channels<br />
Room A2<br />
Tuesday, February 3, 2004<br />
Microfluidics<br />
Room A4<br />
5:00 – 6:30 pm Reception in the Exhibit Hall, Celebrating JALA Authors<br />
7:00 – 9:00 pm GALA Dinner at the Fairmont Hotel<br />
9:00 – 10:30 pm JALA Authors Reception (Invitation Only)<br />
14<br />
Proteomics<br />
Arrays<br />
Room B1<br />
Genomics<br />
Analytical<br />
Room A1<br />
Clinical<br />
Proteomics<br />
Room C1<br />
Engineering<br />
Applications<br />
Room A3
8:00 – 10:00 am<br />
10:30 am – 12:30 pm<br />
HT Chemistry<br />
Room B3<br />
HT Chemistry<br />
Microscale<br />
Room B3<br />
10:00 am – 6:30 pm Exhibits Open<br />
HTS<br />
Informatics<br />
Room A2<br />
HTS Analytical<br />
Room A2<br />
12:30 – 2:00 pm Lunch for Attendees in the Exhibit Hall<br />
Wednesday, February 4, 2004<br />
Microfluidic<br />
Separations<br />
Room A4<br />
Microfluidics<br />
Room A4<br />
Proteomics<br />
Structural<br />
Room B1<br />
Proteomics<br />
Informatics<br />
Room B1<br />
Genomics<br />
Instrumentation<br />
Room A1<br />
Genomics<br />
SNPs<br />
Room A1<br />
12:30 – 2:00 pm JALA Prospective Authors Workshop, Room A8 (Open to all Conference Attendees.)<br />
2:00 – 3:30 pm Poster Session in the Exhibit Hall<br />
3:30 – 5:30 pm<br />
HT Chemistry<br />
New Strategies<br />
Room B3<br />
HTS Data<br />
Analysis & QC<br />
Room A2<br />
5:30 – 6:30 pm Reception in the Exhibit Hall<br />
7:00 – 10:00 pm Exhibitor Move Out<br />
8:00 am – 5:00 pm Exhibitor Move Out<br />
8:00 – 10:00 am<br />
10:30 am – 12:00 pm<br />
HT Chemistry<br />
Informatics<br />
Room B3<br />
ADME – Tox<br />
Room A2<br />
12:00 – 1:30 pm Lunch for Attendees<br />
1:30 – 3:30 pm<br />
HTS<br />
Automated<br />
Design<br />
Room A2<br />
Drug Discovery Case Studies<br />
Room B1<br />
Microfluidics<br />
Detection<br />
Room A4<br />
Proteomics<br />
Technology 1<br />
Room B1<br />
Thursday, February 5, 2004<br />
Microchips<br />
Separations<br />
Room A4<br />
Microfluidics<br />
Bioanalytical<br />
Room A4<br />
Microfluidics<br />
Small Volume<br />
Dispensing<br />
Room A4<br />
4:00 – 5:00 pm Jouan Award Winner Presentation, Room B1<br />
5:00 – 6:00 pm Farewell<br />
15<br />
Proteomics<br />
Technology 2<br />
Room B1<br />
Proteomics<br />
Technology 3<br />
Room B1<br />
Aut<strong>omation</strong><br />
Applications in<br />
Process R&D<br />
Room A2<br />
Genomics<br />
Informatics<br />
Room A1<br />
Genomics<br />
Arrays<br />
Room A1<br />
Genomics<br />
Advanced<br />
Topics<br />
Room A1<br />
Clinical POC<br />
Room C1<br />
Clinical<br />
Molecular<br />
Diagnostics<br />
Room C1<br />
Clinical<br />
Informatics 1<br />
Room C1<br />
Clinical<br />
Informatics 2<br />
Room C1<br />
Clinical Arrays<br />
Room C1<br />
Clinical Pharmacogenomics<br />
Room C1<br />
Emerging<br />
Technology<br />
Room A3<br />
Emerging<br />
Technology<br />
Room A3<br />
Emerging<br />
Technology<br />
Room A3<br />
National Lab<br />
Technology<br />
Transfer<br />
Room A3<br />
Emerging IT<br />
Informatics<br />
Room A3<br />
Performance<br />
Metrics<br />
Room A1<br />
CONFERENCE AT A GLANCE
PROGRAM<br />
Sunday, February 1, 2004<br />
8:30 am – 4:30 pm Short Courses – Pages 298 – 300<br />
Room B1 Barcode Technology<br />
Room C1 Economic Justification of Laboratory Aut<strong>omation</strong><br />
Room B3 Emerging IT for the Laboratory<br />
Room J3 Introduction to High Throughput Screening<br />
Room J2 Introduction to Laboratory Aut<strong>omation</strong><br />
Room C4 Introduction to LabView<br />
Room F Introduction to Molecular Biology<br />
Room B2 Mass Spectrometry Instrumentation & High Throughput Analysis<br />
Room J4 Microarrays & Applications<br />
Room B4 Migrating From VB6 to VB.Net<br />
Two-Day Courses – Page 300<br />
Room C2 Getting Started With Excel and VBA in the Laboratory<br />
Room J1 Microfluidics I & II<br />
Monday, February 2, 2004<br />
8:30 am – 4:30 pm Short Courses – Pages 301 – 303<br />
Room C1 Biostatistics<br />
Room B1 Introduction to Databases in the Laboratory<br />
Room J2 Introduction to Laboratory Aut<strong>omation</strong><br />
Room B4 LIMS in the Organization<br />
Room B2 Mass Spectrometry in Proteomics & Drug Discovery<br />
Room C4 Mathematica<br />
Room J4 Molecular Diagnostic Aut<strong>omation</strong><br />
Room F Patent Law<br />
Room J3 Pharmacogenomic Aut<strong>omation</strong><br />
Room B3 Trajectory of Clinical Laboratory Aut<strong>omation</strong><br />
Two-Day Courses – Page 300<br />
Room C2 Getting Started With Excel and VBA in the Laboratory<br />
Room J1 Microfluidics I & II<br />
4:00 – 8:00 pm Opening Reception in the San Jose McEnery Convention Center Exhibit Hall<br />
Tuesday, February 3, 2004<br />
8:15 – 8:30 am Room A2 Welcome and Opening Remarks<br />
8:30 am – 12:00 pm Room A2 Plenary Session Chair Tony J. Beugelsdijk<br />
8:30 am Changing Patterns in the Discovery of New Drugs; Jürgen Drews, Nigeons GmbH<br />
9:15 am Financial Forces Shaping the Future of the Pharmaceuticals and Biotech Industry;<br />
John D. Rhodes, Deloitte & Touche<br />
10:00 – 10:30 am Break<br />
10:30 am Plenary Session Chair Peter Grandsard<br />
A Look to the Future<br />
Microfluidics in Your Future; Richard A. Mathies, University of California, Berkeley<br />
11:15 am The Nanophysiometer: Holographic Sensors for Drug Discovery; Christopher R. Lowe,<br />
University of Cambridge<br />
12:00 – 1:30 pm Lunch Break in the San Jose McEnery Convention Center Exhibit Hall<br />
1:30 – 3:00 pm Poster Session – List begins on page 25. San Jose McEnery Convention Center Exhibit Hall<br />
3:00 – 5:00 pm Room B3 High Throughput Chemistry<br />
Chair: Michal Lebl, Illumina, Inc.<br />
3:00 pm Natural Products Inspired Studies in Asymmetric Synthesis;<br />
Erick Carreira, Swiss Federal Institute of Technology – ETH Zürich<br />
3:30 pm Solid and Solution-phase Syntheses of Peptidomimetics That Mimic or Disrupt Proteinprotein<br />
Interactions; Kevin Burgess, Texas A & M University<br />
4:00 pm Tailoring Molecular Diversity Through Carbohydrate-based Scaffolds;<br />
Wim Meutermans, Alchemia Pty Ltd<br />
4:30 pm From “One-Bead One-Compound” to Chemical Microarrays;<br />
Jan Marik, University of California, Davis – Cancer Center<br />
16
3:00 – 5:00 pm Room A2 High Throughput Screening Ion Channels<br />
Chair: Tina Garyantes, Aventis, Inc.<br />
3:00 pm Higher Throughput Electrophysiology and Ion Channel Assays Technologies;<br />
Matching Targets, Throughput and Target Prosecution Objectives;<br />
Jennings Worley, Amphora Discovery Corporation<br />
3:30 pm PatchXpress: Automated Patch Clamp for High-quality, High Throughput Recordings of<br />
Both Voltage and Ligand-Gated Ion Channels; Chris Mathes, Axon Instruments, Inc.<br />
4:00 pm Improving Recording Quality for High Throughput Patch Clamp;<br />
Jia Xu, AVIVA Biosciences Corporation<br />
4:30 pm CytoPatch– Automated Patch Clamping; Christa Nutzhorn, CYTOCENTRICS GmbH<br />
3:00 – 5:00 pm Room A4 Microfluidics<br />
Chair: Juan Santiago, Stanford University<br />
3:00 pm Low-voltage, Spatially-localized Electrokinetic Control;<br />
Katherine Dunphy, University of California, Berkeley<br />
3:30 pm Analysis of Microscale Transport for BioMEMS;<br />
Carl Meinhart, University of California, Santa Barbara<br />
4:00 pm Single Molecule Amplification in a Continuous Flow LabChip Device;<br />
Jill Baker, Caliper Technologies Corp.<br />
4:30 pm A Generalized Dispersion Theory Model for Field Amplified Sample Stacking;<br />
Rajiv Bharadwaj, Stanford University<br />
3:00 – 5:00 pm Room B1 Proteomics Arrays<br />
Chair: Bernard Geierstanger, Novartis Research Foundation<br />
3:00 pm High Sensitivity Multiplexed Serum Protein Analysis Using Antibody Microarrays and<br />
Rolling Circle Amplification; Brian Haab, Van Andel Research Institute<br />
3:30 pm Application of Functional Protein Microarrays to Kinase Drug R&D; Paul Predki, Protometrix, Inc.<br />
4:00 pm Protein Array Preparation by Ion Soft-Landing; Zheng Ouyang, Purdue University<br />
4:30 pm New Detection Principles for Protein Biochips; Peter Wagner, Zyomyx, Inc.<br />
3:00 – 5:00 pm Room A1 Genomics Analytical<br />
Chair: Michael Becker, Gen-Probe, Inc.<br />
3:00 pm Miniaturizing the Time-of-Flight Mass Spectrometer While Maintaining High Performance;<br />
Robert Cotter, Johns Hopkins University<br />
3:30 pm Detection and Characterization of Biothreats and Emerging Infectious Diseases – The TIGER<br />
Concept; Steven Hofstadler, Ibis Therapeutics<br />
4:00 pm The TIGRIS System for Total Aut<strong>omation</strong> of Nucleic Acid Testing;<br />
James Godsey, Gen-Probe, Inc.<br />
4:30 pm Multiplex Preamplification of Assays-on-Demand Product Targets Prior to Quantitative<br />
PCR Analysis of Gene Expression in Blood; Mark Shannon, Applied Biosystems<br />
3:00 – 5:00 pm Room C1 Clinical Proteomics<br />
Chair: Daniel W. Chan, Johns Hopkins University<br />
3:00 pm Clinical Proteomics 2004; Daniel W. Chan, Johns Hopkins University<br />
3:30 pm Combining LC and MALDI Mass Spectrometry: A Way to a Simpler Workflow?<br />
Keith Ashman, MDS Sciex<br />
4:00 pm Protein Arrays: Movement Toward Clinical Value; Larry Gold, SomaLogic, Inc.<br />
4:30 pm Current Status of Microdevices in the Clinical Laboratory;<br />
Larry Kricka, University of Pennsylvania Medical Center<br />
3:00 – 5:00 pm Room A3 Engineering Applicatons<br />
Chair: Carl Murray, Beckman Coulter, Inc.<br />
3:00 pm Dynamic Scheduling in the Laboratory – Problems and Solutions;<br />
Reinhold Schäfer, Fachhochschule Wiesbaden<br />
3:30 pm The Data Pipelining Approach to Informatics Aut<strong>omation</strong> and Integration;<br />
Mathew Hahn, Scitegic<br />
4:00 pm Comprehensive Materials Informatics in Chemical and Pharmaceutical Research;<br />
David Dorsett, Symyx Technologies, Inc.<br />
4:30 pm Rapid Evaluation, Normalization and Global Search Program for 2D Electrophoresis;<br />
Robert L. Stevenson, IO Informatics, Inc.<br />
17<br />
PROGRAM
5:00 – 6:30 pm Reception in the San Jose McEnery Convention Center Exhibit Hall, Celebrating JALA Authors<br />
7:00 – 9:00 pm GALA Dinner at the Fairmont Hotel<br />
9:00 – 10:30 pm JALA Authors Reception (Invitation Only)<br />
Wednesday, February 4, 2004<br />
8:00 – 10:00 am Room B3 High Throughput Chemistry<br />
Chair: Andrew Organ, GlaxoSmithKline<br />
8:00 am Aut<strong>omation</strong> and Miniaturization in NMR; Götz Schlotterbeck, F. Hoffmann-La Roche Ltd.<br />
8:30 am Aut<strong>omation</strong> and Mass Spectrometry: A Love – Hate Relationship?<br />
Russell Scammell, Argenta Discovery Ltd.<br />
9:00 am Rapid, High Resolution Multiplexed HPLC System; Dave Rakestraw, Eksigent Technologies<br />
9:30 am Mission Insoluble – Physchem Property Profiling With Rapidity; Chris Bevan, GlaxoSmithKline<br />
8:00 – 10:00 am Room A2 High Throughput Screening Informatics<br />
Chair: John Kochins, GlaxoSmithKline<br />
8:00 am Interpretation of Metabolomic Data Using Explanatory Machine Learning; Roy Goodacre, UMIST<br />
8:30 am Bayesian Approaches to the Analysis of High Throughput Experimental Data: Using What We<br />
Know to Discover What We Don’t; Nick Haan, BlueGnome, Ltd.<br />
9:00 am Getting the Most From Your HTS: Quality Control and Data Mining;<br />
Ramesh Padmanabha, Bristol-Myers Squibb Co.<br />
9:30 am Information Technology for High Content Screening of Miniaturized Cellular Assays;<br />
Martin Daffertshofer, Evotec Technologies GmbH<br />
8:00 – 10:00 am Room A4 Microfluidics Separations<br />
Chair: Jörg P. Kutter, Technical University of Denmark<br />
8:00 am On the Use of Nano-Channel Flows for the Enhancement of Micro-Analytical Separations;<br />
Johan Vanderhoeven, Free University of Brussels<br />
8:30 am Miniaturized Field Inversion Electrophoresis; H. John Crabtree, Micralyne, Inc.<br />
9:00 am Micro Parallel Liquid Chromatography System for High Throughput Analysis;<br />
Steve Hobbs, Nanostream<br />
9:30 am Polymer-based Microfluidic Systems for the High Resolution Separation of Nucleic Acids:<br />
Applications in DNA Diagnostics and Sequencing; Steven A. Soper, Louisiana State University<br />
8:00 – 10:00 am Room B1 Proteomics Structural<br />
Chair: Duncan Mcree, ActiveSight<br />
8:00 am Advanced Mixology: Liquid Handling Devices for High Speed Cocktail Preparation and<br />
Iterative Protein-ligand Co-crystallization Experiments; Lance Stewart, deCODE genetics, Inc.<br />
8:30 am High Throughput Technologies in Structural Biology and Applications Towards Genomes,<br />
Pathways, and Drug Design; Frank Von Delft, The Scripps Research Institute<br />
9:00 am Automated Protein Crystallization System; John A. Adams, RoboDesign International, Inc.<br />
9:30 am Automated Combinatorial Protein Crystallization Screening;<br />
Brent Segelke, Lawrence Livermore National Laboratory<br />
8:00 – 10:00 am Room A1 Genomics Instrumentation<br />
Chair: Scott Hunike-Smith, Ambion, Inc.<br />
8:00 am Parallab Technology: Integrated Nanoliter Genomic Workstation;<br />
Steven Gordon, Brooks-PRI Aut<strong>omation</strong><br />
8:30 am A Novel GenVault Multiwell Plate Format for Whatman FTA in Robotic, High Throughput DNA<br />
Archiving; Jim Davis, GenVault Corporation<br />
9:00 am Electrostatic Printing of Composite Features for Highly Uniform DNA Microarrays;<br />
Kurtis Guggenheimer, University of British Columbia<br />
9:30 am A New Dimension in Routine Genomic and Proteomic Analyses; Ram Vairavan, AutoGenomics<br />
8:00 – 10:00 am Room C1 Clinical POC<br />
Chair: James Nichols, Baystate Health System<br />
8:00 am Reducing Medical Errors at the Point-of-Care; James Nichols, Baystate Health System<br />
8:30 am Implementation of POC Web Connectivity; Randall Roy, Affiliated Laboratory, Inc.<br />
9:00 am Clinical Outcomes From Point of Care Connectivity; Louis Dunka, LifeScan<br />
9:30 am Compliance, Connectivity, and POCT; Frederick Kiechle, William Beaumont Hospital<br />
8:00 – 10:00 am Room A3 Emerging Technology Cell Based Screening Approaches<br />
Chair: Stewart Chipman, SDC Associates, Inc.<br />
8:00 am Novel Cellular Analysis Platform for Drug Discovery; Evan Cromwell, Blueshift Biotechnologies, Inc.<br />
8:15 am Screening for Ion Channel Modulators Using the IonWorks HT System;<br />
James Costantin, Molecular Devices Corp.<br />
18
8:30 am Dynamic Data Mining and High Throughput Microscopy Improve Productivity of Assay<br />
Design and Screening; Jeffrey Price, University of California, San Diego<br />
8:45 am Development of a Mammalian Cell Colony Imaging and Picking Robot: ClonePix;<br />
Steve Richmond, Genetix Ltd.<br />
9:00 am 96-well Protein-Binding Studies in Drug Discovery;<br />
Rowan Stringer, Novartis Horsham Research Centre<br />
9:15 am A Drug Discovery Screen and Chemical-Genetic Approach for Novel Apoptosis Inducers;<br />
Ben Tseng, Maxim Pharmaceuticals<br />
9:30 am Detection of BD Living Colors Novel Flourescent Proteins Using the Acumen Explorer;<br />
Matthew Cook, TTP Lab-Tech<br />
9:45 am Automated Molecular Haplotyping Through Physical Separation of DNA;<br />
Johannes Dapprich, Generation Biotech, LLC<br />
10:00 – 10:30 am Break<br />
10:30 am – 12:30 pm Room B3 High Throughput Chemistry Microscale<br />
Chair: Mark Bradley, University of Southampton<br />
10:30 am Microfluidic Platforms for Drug Discovery; Miryam Fernandez Suarez, GlaxoSmithKline<br />
11:00 am Real-world Microchemistry: Milligram Scale Organic Synthesis in Microreactors;<br />
Mike Pollard, Syrris<br />
11:30 am Microscale Synthesis – Integration of Reactions and Separations;<br />
Klavs Jensen, Massachussetts Institute of Technology<br />
12:00 pm CYTOS Continuous Chemistry – A Coherent Chemical Synthesis Technology to<br />
Lever Drug Innovation Process Value Generation;<br />
Thomas Schwalbe, CPC – Cellular Process Chemistry Systems GmbH<br />
10:30 am – 12:30 pm Room A2 High Throughput Screening Analytical<br />
Chair: Steven A. Hofstadler, Ibis Therapeutics, Inc.<br />
10:30 am HTAPlate: Unique 96-Well Plate for DNA- and Protein Arrays; Jörg Stappert, Greiner Bio-One<br />
11:00 am Accelerating Discovery Analytical Chemistry Through Micro Parallel Liquid Chromatography;<br />
Paren Patel, Nanostream<br />
11:30 am DMSO Hydration Monitoring by Ultrasound in Compound Library Microplates and<br />
Implications for Tracking Compound Dilution; Richard Ellson, Picoliter Inc.<br />
12:00 pm Integration of Hydrogel Based Bioassays Into Microfluidic Channels;<br />
Rebecca Zangmeister, National Institute of Standards and Technology<br />
10:30 am – 12:30 pm Room A4 Microfluidics<br />
Chair: Michael Spaid, Caliper Technologies Corp.<br />
10:30 am Electrokinetic Flow Instabilities; Juan Santiago, Stanford University<br />
11:00 am A Microfluidics Approach to Protease Substrate Identification;<br />
Christopher Tsu, Millennium Pharmaceuticals<br />
11:30 am Microfluidic Chips for Time-Resolved High Throughput Electrophysiology;<br />
Daniel Chiu, Cellectricon<br />
12:00 pm BIOMEMS Solutions at Silex Microsystems; Helene Andersson, Silex Microsystems<br />
10:30 am – 12:30 pm Room B1 Proteomics Informatics<br />
Chair: Chris Herold, Prediction Sciences and Omniomix<br />
10:30 am The Automated Biomarker System: A High Throughput Proteomics Biomarker Discovery<br />
Platform; Eric Fung, Ciphergen Biosystems, Inc.<br />
11:00 am High Throughput Protein Domain Elucidation by Limited Proteolysis-Mass Spectrometry;<br />
Xia Gao, Structural GenomiX, Inc.<br />
11:30 am Protein Variation SAR Using ActivityBase; Jost Vielmetter, Xencor<br />
12:00 pm Strategic Opportunities in the High-Content Screening and Computational Proteomics;<br />
Enal Razvi, DiscoveRx Corporation<br />
10:30 am – 12:30 pm Room A1 Genomics SNPs<br />
Chair: Tom Willis, ParAllele BioScience, Inc.<br />
10:30 am Aut<strong>omation</strong> of Amplification and Primer Extension Chemistries for Beckman Coulter’s<br />
SNPstream ® Genotyping System; Keith Roby, Beckman Coulter, Inc.<br />
11:00 am Sensitive Detection and Identification of Threat Agents by Microarray-based Genotyping<br />
Using MLST Derived Signatures; Robert B. Cary, Los Alamos National Laboratory<br />
11:30 am Improvements on the TDI-FP SNP Genotyping Assay; Ming Xiao; University of California,<br />
San Francisco Cardiovascular Research Institute<br />
12:00 pm Comprehensive Genetic Analysis Using Highly Multiplexed SNP Discovery and Genotyping;<br />
Tom Willis, ParAllele BioScience, Inc.<br />
19<br />
PROGRAM
Wednesday, February 4, 2004 (continued)<br />
10:30 am – 12:30 pm Room C1 Clinical Molecular Diagnostic<br />
Chair: Patrick Merel, CHU Pellegrin<br />
10:30 am NAT Aut<strong>omation</strong> – Enabling Transformation of Molecular Technology From Research to IVD;<br />
Guido Baechler; Roche Molecular Systems<br />
11:00 am High Throughput Molecular Infectious Diseases Tests in the Routine Clinical Laboratory<br />
Environment; Hasnah Hamdan, Quest Diagnostics Nichols Institute<br />
11:30 am Process Control With Automated NAAT Systems; Jeffrey Allen, Gen-Probe, Inc.<br />
12:00 pm Primary DNA-based Newborn Screening of Sickle Cell Disease and Hemoglobinopathy;<br />
Zhili Lin, Pediatrix Screening, Inc.<br />
10:30 am – 12:30 pm Room A3 Emerging Technology Hardware<br />
Chair: Steve Fillers, TekCel, Inc.<br />
10:30 am Optimizing Surfaces for Assays in Plates, on Luminex Beads and Microarrays Using<br />
Combinatorial Chemistry Customized Surface Coatings; Jason Armstrong, Polymerat<br />
10:45 am Development of Reduction-Oxidation Magnetohydrodynamic Devices With Integrated<br />
Permanent Magnets; Prabhu U. Arumugam, University of Arkansas<br />
11:00 am Label-Free Assays Using the BIND System; Brian Cunningham, SRU Biosystems<br />
11:15 am A Flexible Substrate for DNA Microarray Hybridization and Detection Using a Business Card<br />
Scanner; Martin Dufva, Mikroelektronik Centret<br />
11:30 am Merging Highest Resolution and High Speed: Instrumentation for HTS Cell Assays and Image<br />
Activated Cell Sorting; Gabriele Gradl, Evotec Technologies<br />
11:45 am TipCharger ® Cleaning Technology: Status and Laboratory Performance of the Technology;<br />
Paul Hensley, Microplate Aut<strong>omation</strong><br />
12:00 pm SmartPlate Implementation and Return on Investment Examples for Compound<br />
Management; Jeff Karg, Boston Innovation<br />
12:15 pm Using Data Collected in Real Time From Liquid Transfer Operations to Audit and Enhance<br />
Performance; Hakki Unver, Zymark Corporation<br />
12:30 – 2:00 pm Room A8 JALA Prospective Authors Workshop (Open to all Conference Attendees.)<br />
12:30 – 2:00 pm Lunch Break in the San Jose McEnery Convention Center Exhibit Hall<br />
2:00 – 3:30 pm Poster Session – List begins on page 25. San Jose McEnery Convention Center Exhibit Hall<br />
3:30 – 5:30 pm Room B3 High Throughput Chemistry New Strategies<br />
Chair: Nicholas Hird, Takeda Chemical Industries, Ltd.<br />
3:30 pm Real Time Combinatorial Arrays and Assays for Proteases, Kinases, and Transfection Agents;<br />
Mark Bradley; University of Southampton<br />
4:00 pm High Throughput Drug Discovery; Thomas Smith, GlaxoSmithKline<br />
4:30 pm New Approaches for High Throughput Heterocycle Synthesis;<br />
Jonathan Ellman, University of California, Berkeley<br />
5:00 pm Aut<strong>omation</strong> of Bioanalytical Processes Using the Lab-on-Valve Approach. Applications<br />
to Protein Binding and Diagnosis of Inborn Errors of Metabolism;<br />
Frantisek Turecek, University of Washington<br />
3:30 – 5:30 pm Room A2 High Throughput Screening Data Analysis and QC<br />
Chair: Ji-Hu Zhang, Novartis Institutes for BioMedical Research<br />
3:30 pm Evotec uHTS Data Analysis – Making the Most of the Multiparametric Readout;<br />
Anthony Carlo, Pfizer<br />
4:00 pm HTS Data Analysis in the Real World: Practical Experience With HTS Data Quality<br />
Assurance Systems and Recent Integration of the GeneData Screener Software;<br />
Hanspeter Gubler, Novartis Institutes for BioMedical Research<br />
4:30 pm On-Line QC for High Throughput Screening; Maneessha Altekar, GlaxoSmithKline<br />
5:00 pm Finding and Correcting Systematic Bias in Array Experiments; John Elling, Datect, LLC<br />
3:30 – 5:30 pm Room A4 Microfluidics Detection<br />
Chair: Daryl Bornhop, Vanderbilt University<br />
3:30 pm Automating a Multichamber, Multianalyte Microphysiometer for Metabolic Responses Using<br />
Labview; David Cliffel, Vanderbilt University<br />
4:00 pm Heterostructures of Nanomaterials and Organic-Inorganic Nanoassemblies;<br />
Cengiz S. Ozkan, University of California, Riverside<br />
4:30 pm Surface Enhanced Raman Spectroscopy for Real World Samples;<br />
Wayne Weimer, US Detection Technologies<br />
5:00 pm Advances in Electrochemical Detection of Neurotransmitters in Microchannels;<br />
R. Scott Martin, Saint Louis University<br />
20
3:30 – 5:30 pm Room B1 Proteomics Technology I<br />
Chair: Sheri Wilcox, SomaLogic, Inc.<br />
3:30 pm Use of Beckman Coulter’s Biomek FX to Automate Epitope Discovery for Specific Antigens<br />
and Determine Optimal Peptides for Major Histocompatibility Complex (MHC) Class I Binding;<br />
Judith Finlay, Beckman Coulter, Inc.<br />
4:00 pm Active Arrays – Time Resolved Analysis in Microarrays for Binding Kinetics and Enzymatic<br />
Activities; Nenad Gajovic-Eichelmann, Fraunhofer Institute Biomedical Engineering<br />
4:30 pm Probing Multicomponent Protein Assemblies Using Site-Directed Attachment of Fluorophores<br />
and Crosslinking Agents; Philip E. Dawson, The Scripps Research Institute<br />
5:00 pm A Systems Biology Approach to Tracking Protein/Gene Expression and Interactions in Oncology<br />
and Toxicology Using the eTagAssay System; Travis Boone, ACLARA BioSciences, Inc.<br />
3:30 – 5:30 pm Room A1 Genomics Informatics<br />
Chair: Paul Kayne, Bristol-Myers Squibb Co.<br />
3:30 pm The Benefits of a Laboratory Information Management System (LIMS) in Genomics;<br />
Terrence Smallmon, LabVantage Solutions<br />
4:00 pm Feeding a Multiplatform Genome Center; Andrei Verner, McGill University and Genome<br />
Quebec Innovation Centre<br />
4:30 pm Open Interfacing for Lab Aut<strong>omation</strong>; Roger McIntosh, Silicon Valley Scientific<br />
5:00 pm The Changing Face of Lab Aut<strong>omation</strong>; Dave Riling, DataCentric Aut<strong>omation</strong><br />
3:30 – 5:30 pm Room C1 Clinical Informatics I<br />
Chair: William Neeley, Detroit Medical Center<br />
3:30 pm Autoverification: Paving the Path to Efficiency and Quality; John Saulenas, MEDITECH<br />
4:00 pm Autoverification of Laboratory Results – A Real Life Perspective;<br />
Leo Serrano, West Tennessee Healthcare<br />
4:30 pm Autoverification and Regulatory Issues: The Rules and Regulations According to CLIA, CAP, and<br />
the California Department of Health Services; David Velasquez, Mills-Peninsula Health Services<br />
5:00 pm Post-Analytic Intelligent Systems vs. Autoverification: The Future;<br />
William Neeley, Detroit Medical Center<br />
3:30 – 5:30 pm Room A3 Emerging Technology Aut<strong>omation</strong> Systems<br />
Chair: Jeff Karg, Boston Innovation, Inc.<br />
3:30 pm The ArrayPlate: A Novel, High Throughput, Multiplexed, mRNA Assay for Toxicological<br />
Research and Development; Bruce Seligmann, High Throughput Genomics, Inc.<br />
3:45 pm Temperature Gradient Focusing, Modeling, and Experiments; David Huber, Stanford University<br />
4:00 pm An Automated Flow Cytometry Quality Protocol and Workflow System for Managing<br />
Instruments, Samples, and Data; Sanjaya Joshi, Userspace Corporation<br />
4:15 pm Vertical Integration Platform; Emir Osmanagic, Scinomix<br />
4:30 pm Automated Accurate Mass Analysis Using FTICR Mass Spectrometry;<br />
Bradley Mikesell, Pfizer Global R&D<br />
4:45 pm Sequential Organic Synthesis as a New Approach in Drug Discovery;<br />
Donald Mossman, CPC-Cellular Process Chemistry, Inc.<br />
5:00 pm Managing Automated RFLP Analysis in Phage Display Antibody Screening;<br />
Jaymie Sawyer, Dyax Corporation<br />
5:15 pm The Next Generation of a Compound Management System in Drug Discovery;<br />
David Semin, Amgen, Inc.<br />
5:30 – 6:30 pm Reception in the San Jose McEnery Convention Center Exhibit Hall<br />
Thursday, February 5, 2004<br />
8:00 – 10:00 am Room B3 High Throughput Chemistry Informatics<br />
Chair: David Nirschl, Bristol-Myers Squibb Co.<br />
8:00 am High Throughput Quality Control LC-MS Analysis: Data Acquisition and Interpretation;<br />
Bernard Choi, Merck Research Laboratories<br />
8:30 am Navigating Large Chemical Spaces; Tudor Oprea, University of New Mexico<br />
9:00 am An Informatics System for Peptide Drug Discovery; Ping Du, Du Consulting, LLC<br />
9:30 am Process Definition and Evaluation Utilizing Automated Metrics Gathering;<br />
Neil Benn, Cambridge Antibody Technology Limited<br />
8:00 – 10:00 am Room A2 High Throughput Screening Automated Design<br />
Chair: Paul Taylor, Boehringer Ingelheim Pharmaceuticals, Inc.<br />
8:00 am Efficient Protein Crystallization; Larry DeLucas, University of Alabama at Birmingham<br />
8:30 am The Implementation of Statistical Design of Experiments (DOE) in the Construction of Assays<br />
and High Throughput Screens; Normand Cloutier, Bristol-Myers Squibb Co.<br />
21<br />
PROGRAM
Thursday, February 5, 2004 (continued)<br />
9:00 am The Application of Design of Experiment in Developing Processes for Drug Discovery;<br />
W. Adam Hill, Millennium Pharmaceuticals<br />
9:30 am Automated Cell-based Assay Optimization by Design of Experiment; Amy Siu, GlaxoSmithKline<br />
8:00 – 10:00 am Room A4 Microchip Separations<br />
Chair: Steve Soper, Louisiana State University<br />
8:00 am Capillary Electrochromatography Chip Featuring Sub-micron “Channels” and Integrated<br />
Waveguides; Jörg P. Kutter, Technical University of Denmark<br />
8:30 am 2D PAGE on a Chip: Multidimensional Protein Separations via High Throughput Microfluidics;<br />
Don L. DeVoe, Calibrant BioSystems, Inc.<br />
9:00 am High Throughput Preparation of Nanoscale Samples for Capillary Array Electrophoresis and<br />
a Microchip-based Analysis System; Stevan Jovanovich, Silicon Valley Scientific<br />
9:30 am A Comparison of Pressure Cycling Technology (PCT) to Standard Laboratory Techniques for<br />
the Release of Nucleic Acids and Proteins From a Variety of Difficult-to-lyse Sample Types;<br />
Nathan Lawrence, Boston Biomedica, Inc.<br />
8:00 – 10:00 am Room B1 Proteomics Technology 2<br />
Chair: Paul A. Haynes, University of Arizona<br />
8:00 am The Finnigan LTQ-FT and Shotgun Proteomics; David Goodlett, ISB<br />
8:30 am Rapid and Reproducible Fractionation and Identification of Proteins Using<br />
Membrane Chromatography and Orthogonal MALDI-TOF Mass Spectrometry;<br />
Mary F. Lopez, PerkinElmer Life and Analytical Sciences<br />
9:00 am Polyclonal Gene-Specific IgY Antibodies for Proteomics and Abundant Plasma Protein<br />
Depletion; Wei-Wei Zhang, GenWay Biotech, Inc.<br />
9:30 am Protease Substrate Profiling Using Microarrays; Dhaval N. Gosalia, University of Pennsylvania<br />
8:00 – 10:00 am Room A1 Genomics Arrays<br />
Chair: Robert Cary, Los Alamos National Laboratory<br />
8:00 am Automated Aptamer Selection: Applications in RNA: Protein Sequence Specificity, and<br />
Aptamer-Chip Microarrays; J. Colin Cox, University of Texas<br />
8:30 am Microtape and Associated Instrumentation for Continuous Array High Throughput Genotyping;<br />
Jon Chudyk, Marshfield Clinic Research Foundation<br />
9:00 am Immunoassays and Sequence-Specific DNA Detection on a Microchip Using Enzyme<br />
Amplified Electrochemical Detection; Andrey Ghindilis, CombiMatrix Corporation<br />
9:30 am Nanoarrays – A Bottom-Up Method to Create Nanometer Addressable Lateral Surface<br />
Structures by use of Nucleic Acids; Frank F. Bier, Fraunhofer Institute for Biomedical Engineering<br />
8:00 – 10:00 am Room C1 Clinical Informatics 2<br />
Chair: Jay Jones, Geisinger Health System<br />
8:00 am The Role of Information Systems in Aut<strong>omation</strong> in a Large Reference Laboratory;<br />
Charles Hawker, ARUP Laboratories, Inc.<br />
8:30 am Clinical Informatics – “Middleware”; Marcy Anderson, Medical Aut<strong>omation</strong> Systems<br />
9:00 am Middleware Solutions – Optimizing People, Processes, and Platforms;<br />
Hunter Bagwell, Roche Diagnostics<br />
9:30 am Data Mining From the LIS With Simple PC Tools; Jay Burton Jones, Geisinger Health System<br />
8:00 – 10:00 am Room A3 Emerging Technology National Lab Tech Transfer<br />
Chair: Erica Briggs, Los Alamos National Laboratory<br />
8:00 am Introduction<br />
8:15 am Technology Transfer at Argonne National Laboratory; Steve Lake, Argonne National Laboratory<br />
8:30 am Technology Transfer at Los Alamos National Laboratory;<br />
Erica Briggs, Los Alamos National Laboratory<br />
8:45 am Technology Transfer at Lawrence Berkeley National Laboratory;<br />
Cheryl Fragiadakis, Lawrence Berkeley National Laboratory<br />
9:00 am Technology Transfer at Pacific Northwest National Laboratory;<br />
Bruce Harrer, Pacific Northwest National Laboratory<br />
9:15 am Technology Transfer at Sandia National Laboratories;<br />
Paul Dressendorfer, Sandia National Laboratories<br />
9:30 am Panel Discussion and Questions<br />
10:00 – 10:30 am Break<br />
22
10:30 am – 12:00 pm Room A2 ADME – Tox<br />
Chair: Elizabeth Everitt, Abbott Laboratories<br />
10:30 am Role of Automated High Throughout Nephelometric Aqueous Solubility System in<br />
Early Drug Discovery; Arun Mandagere, Pfizer Global R&D<br />
11:00 am Higher Throughput Strategies for Support of Early ADME In-vitro Screening;<br />
Ken Matuszak, Abbott Laboratories<br />
11:30 am The Role of Genomics in Toxicology; Alexandra Heinloth, NIEHS<br />
10:30 am – 12:00 pm Room A4 Microfluidics Bioanalytical<br />
Chair: Laurie Locascio, National Institute of Standards and Technology<br />
10:30 am An Autonomous PCR-based Air Monitoring System to Detect and Identify Infectious Agents;<br />
Phillip Belgrader, Microfluidic Systems, Inc.<br />
11:00 am Protein Sizing and Relative Quantitation Determination Using a Microfluidic LabChip ® Device;<br />
Adrian Winoto, Caliper Technologies Corp.<br />
11:30 am Using Liposomes for High-Efficiency Mixing in Microfluidic Systems;<br />
Laurie Locascio, National Institute of Standards and Technology<br />
10:30 am – 12:00 pm Room B1 Proteomics Technology 3<br />
Chair: Joseph Loo, University of California, Los Angeles<br />
10:30 am Sample Preparation Tips for Sample Enrichment and Direct Elution Nanoelectrospray<br />
Mass Spectrometry Analysis for Enhanced Sensitivity for Protein Characterization;<br />
Gary Schultz, Advion BioSciences, Inc.<br />
11:00 am Progress in Automating Top Down Proteomics; Neil Kelleher, University of Illinois<br />
11:30 am A View of the Proteome Provided by New Mass Spectrometry Technologies;<br />
Joseph Loo, University of California, Los Angeles<br />
10:30 am – 12:00 pm Room A1 Genomics Advanced Topics<br />
Chair: Gary Nunn, Applied Biosystem<br />
10:30 am Increasing Instrumentation Availability by Using Reliability Centered Maintenance (RCM)<br />
Principles and Applying Them to Production Line Instrumentation at the DOE Joint Genome<br />
Institute; Simon Roberts, DOE Joint Genome Institute<br />
11:00 am Evaluation of High Throughput SNP Genotyping Lab Data Using FOCUS Statistical Software;<br />
Sheri Olson, Applied Biosystem<br />
11:30 am Future Clinical Analysis: Component Based Framework for Modular Hardware and Software<br />
Integration of Different Clinical Equipment; Ralf Muckenhirn, Fraunhofer IPA<br />
10:30 am – 12:00 pm Room C1 Clinical Arrays<br />
Chair: William Wachsman, University of California, San Diego<br />
10:30 am Using Reference Gene Expression Datasets to Make Sense of Your Array Data;<br />
John Walker, GNF<br />
11:00 am Molecular Tools Designed for the Clinic; John Palma, Affymetrix, Inc.<br />
11:30 am Clinical Validation of the Affymetrix Microarray and the Challenges Faced;<br />
Richard Hockett, Eli Lilly and Company<br />
10:30 am – 12:00 pm Room A3 Emerging IT Informatics<br />
Chair: Jay Gill, Bristol-Myers Squibb Co.<br />
10:30 am Design Tools: New Approach to Computing in Life Sciences; Larry Arnstein, Teranode Corporation<br />
11:00 am Python for Scientific Computing – An Open Source Solution; Eric Jones, Enthought, Inc.<br />
11:30 am Universal and Mobile Messaging Framework M2A “Message to Anywhere” for Laboratory<br />
Aut<strong>omation</strong>; Gerald Knoll, Fraunhofer Institute Manufacturing Engineering and Aut<strong>omation</strong> (IPA)<br />
12:00 – 1:30 pm Lunch Break<br />
1:30 – 3:30 pm Room B1 Drug Discovery Case Studies<br />
Chair: Jürgen Drews, Nigeons GmbH<br />
1:30 pm Hit and Lead Generation Beyond High Throughput Screening;<br />
Alexander Alanine, F. Hoffmann-La Roche Ltd<br />
2:00 pm Alliances in Technology; Esteban Pombo-Villar, Novartis Pharma Ltd.<br />
2:30 pm Understanding Risk and Value: Decision Gates in Drug Development;<br />
Fred Pritchard, MDS Pharma Services<br />
3:00 pm Drug Prototypes – What We Learn From History;<br />
Walter Sneader, Strathclyde Institute for Biomedical Science<br />
23<br />
PROGRAM
Thursday, February 5, 2004 (continued)<br />
1:30 – 3:30 pm Room A4 Microfluidics Small Volume Dispensing<br />
Chair: Anne Kopf-Sill, Caliper Technologies Corp.<br />
1:30 pm Proteomic Sample Preparation Using Piezo Dispensing and Capillary Force Driven Flow;<br />
Johan Nilsson, Lund University<br />
2:00 pm Differential Nano-Pipettor (NanoBlast): A Non-contact Pipettor That Handles Nanoliters for<br />
Plate Replication, Sample Transfers, Spotting, and Arrays; Donald Schwartz, DRD Diluter Corp.<br />
2:30 pm An Electrochemical Pumping System for On-Chip Gradient Generation;<br />
Terry D. Lee, Beckman Research Institute of the City of Hope<br />
3:00 pm Fully Automated Nanoelectrospray With a BioMEMS Device;<br />
Thomas Corso, Advion BioSciences, Inc.<br />
1:30 – 3:30 pm Room A2 Aut<strong>omation</strong> Applicatons in Process R & D<br />
Chair: Erik Rubin, Bristol-Myers Squibb Co.<br />
1:30 pm Microreactor Systems for Life Science Application; Norbert Stoll, University of Rostock<br />
2:00 pm Accelerating Polymorph and Salt Form Selection; Kara Rubin, Merck Research Laboratories<br />
2:30 pm MeDuSA, An Automated HPLC Screening Tool for PR&D; Brent Karcher, Bristol-Myers Squibb Co.<br />
3:00 pm Parallel Screening and Optimization of Catalytic High-Pressure Reactions;<br />
Rick Sidler, Merck Research Laboratories<br />
1:30 – 3:30 pm Room C1 Clinical Pharmacogenomics<br />
Chair: Charles Hawker, ARUP Laboratories<br />
1:30 pm Potential Role of Pharmacogenomics in Reducing Risk of Adverse Reactions and Optimizing<br />
Therapy; Audrey Papp, Ohio State University<br />
2:00 pm Population Studies Using Enhanced Version of the CodeLink P450 SNP Bioarrays Yield New<br />
and Novel Genotype and Haplotype Frequency Data; Carl Yamashiro, Amersham Biosciences<br />
2:30 pm In-depth Genetic Variation Screening Using DHPLC: A Valuable Component of the Drug<br />
Development Process; Stan Lilleberg, Transgenomic, Inc.<br />
3:00 pm Utilization of Pharmacogenomics in Patient Care for Pain Management Clinics and Poison<br />
Control Centers; Elvan Laleli-Sahin, Medical College of Wisconsin-Milwaukee<br />
1:30 – 3:30 pm Room A1 Performance Metrics<br />
Chair: Henry Schultz, Amgen, Inc.<br />
1:30 pm A Multichannel Volumetric Verification (MVV) System for Ensuring the Accuracy and<br />
Precision of Liquid Delivery; John Bradshaw, Artel, Inc.<br />
2:00 pm Improving Sample Analysis Throughput and Quality With a C#.NET-based, Real-Time QC<br />
Decision Support System; Toshiyuki Shiina, Los Alamos National Laboratory<br />
2:30 pm Application of Integrated Statistical Design and Analysis to Automated Assay Optimization<br />
(AAO); Paul Taylor, Boehringer Ingelheim<br />
3:00 pm Building Confidence in Aut<strong>omation</strong>; Peter Grandsard, Amgen, Inc.<br />
3:30 – 4:00 pm Break<br />
4:00 – 5:00 pm Room B1 Jouan Award Winner Presentation<br />
Chair E. Kevin Hrusovsky<br />
4:00 pm Chip Based High Throughput Analysis; Andreas Manz, ISAS, Germany<br />
5:00 – 6:00 pm Farewell<br />
24
POSTER PROGRAM<br />
TP – TUESDAY POSTERS should be set up on Tuesday 10:00 – 10:30 am and removed<br />
by 6:30 pm on Tuesday. The presenting author must be present 1:30 – 3:00 pm on Tuesday. Page 27<br />
WP – WEDNESDAY POSTERS should be set up on Wednesday 10:00 – 10:30 am and removed<br />
by 6:30 pm on Wednesday. The presenting author must be present 2:00 – 3:30 pm on Wednesday. Page 34<br />
25<br />
PROGRAM | POSTER PROGRAM LISTING
NOTES<br />
26
These posters should be put up on Tuesday by 10:30 am and removed by 6:30 pm on<br />
Tuesday. Authors must be present 1:30 – 3:00 pm on Tuesday.<br />
TP001 Sintalyzer – A Fully Automated Analytical System for Fluoride Analysis<br />
Thor Anders Aarhaug, SINTEF<br />
Co-Author: Kalman Nagy<br />
TP002 The Aut<strong>omation</strong> of TempliPhi and GenomiPhi on the Tecan Genesis Freedom<br />
Monica Adams, Amersham Biosciences<br />
TP003 Aut<strong>omation</strong> of Apoptosis and Reporter-Gene Assays Using Division-Arrested<br />
NFkB HEK293 Cells<br />
Edward Alderman, Zymark Corporation<br />
Co-Authors: Geoffrey N. Grove, Zymark Corporation; Mei Cong and Zhong Zhong, Cell &<br />
Molecular Technologies; Chris Cowan, Promega; Casey Laris, Q3DM<br />
TP004 Automating Aqueous Compound Solubility Screening<br />
Chris Barbagallo, Millipore Corporation<br />
Co-Authors: Greg Kazan, Libby Kellard, Jason Blodgett, and Alan Weiss<br />
TP005 Denaturing Solid-Phase Extraction for Reduced Protein Interference in<br />
Bioanalytical SPE-LC-MS<br />
Alex Berhitu, Spark Holland, Inc.<br />
Co-Authors: Emile Koster, Peter Ringeling, and Bert Ooms<br />
TP006 Meeting the Increasing Challenges of Speed, Performance, and Quality While<br />
Reducing Costs in Whole Genome Sequencing<br />
Emily Berlin, Pall Life Sciences<br />
Co-Authors: Michael L. Metzker, Luke Mast, Geoffrey Okwuonu and Toni Garner, Pall Life<br />
Sciences; Kamran Usmani and Richard A. Gibbs, Baylor College of Medicine<br />
TP007 A Microtube Rack Decapper/Sorter for use in the Automated Preparation of<br />
Compounds for High Throughput Screening<br />
Christopher Bernard, Bristol-Meyers Squibb Co.<br />
Co-Authors: Moneesh Chatterjee, Andrew Bullen, James Myslik, William Monahan, Jeffrey<br />
Guss, Christian Strom, Jay Stevenson, and Alastair Binnie<br />
TP009 Production & Storage of High Density Chemical Microarrays<br />
Stefan Betz, Kendro Laboratory Products GmbH<br />
Co-Author: Michael Frank, Graffinity Pharmaceuticals AG<br />
TP010 PNA-encoded Peptide Libraries - A New Tool in High Throughput Screening<br />
Laurent Bialy, University of Southampton<br />
Co-Author: Mark Bradley<br />
TP011 Adaptation of a High Sensitivity 384-Well Solid Phase Assay Platform to 1536-Well:<br />
CatchPoint for Cyclic Nucleotides and Tyrosine Kinase Activity<br />
Annegret Boge, Molecular Devices<br />
Co-Authors: Jonathan Petersen, Jeannie Nguyen, Gayle Teixeira, and Richard Sportsman<br />
TP012 A Homogeneous Assay for P-glycoprotein Inhibition Using the Acumen Explorer<br />
Wayne Bowen, TTP LabTech<br />
Co-Authors: Tristan Cope and Matthew Cook<br />
TP013 SOS – A Sample Ordering System to Deliver “Assay-Ready” Compound Plates<br />
for Screening<br />
Christine Brideau, Merck Frosst Centre for Therapeutic Research<br />
Co-Authors: Louis Jacques Fortin, Shiraz Adam and Jerry Ferentinos, Merck Frosst Centre<br />
for Therapeutic Research; Joel Hunter, RTS Enabling Technology; and Julio Maher, RTS Life<br />
Science International<br />
27<br />
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POSTER PROGRAM LISTING
TP014 A Novel System for Automated RNA Isolation “Increasing Throughput Without<br />
Increasing Footprint”<br />
Jimmy Bruner, GlaxoSmithKline<br />
Co-Authors: Bryan Laffite, David Murray, Ginger Smith, Jim Liacos, Amy Siu,<br />
and Cathy Finlay<br />
TP015 Development of a Data-driven, Integrated Aut<strong>omation</strong> Platform for High Throughput<br />
Expression and Screening of Protein Engineering Libraries<br />
Jesse Campbell, Applied Molecular Evolution<br />
Co-Authors: Michael J. Cohen, Andrew Korytko, Barbara Swanson, and Alain P. Vasserot<br />
TP016 An Inventory Management Solution From Registration to Assays<br />
Julie Chang, MDL Information Systems<br />
TP017 Rapid Fabrication of Poly(methylmethacrylate) Microfluidic Chips by<br />
Atmospheric Molding<br />
Madhu Prakash Chatrathi, New Mexico State University<br />
Co-Authors: Joseph Wang, Alexander Muck, Scott Spillman, Gautham Sridharan and<br />
Michael Jacobs, New Mexico State University; Michael Schonning, Institute of Thin Films<br />
and Interfaces, Jülich, Germany<br />
TP018 Validation of the Packard Multiprobe HT for Dilution of Biological Matrix Samples<br />
Melissa Cheu, Genentech, Inc.<br />
Co-Authors: Brent T. Nakagiri, Riddhi Patel, and Patricia Y. Siguenza<br />
TP019 Protein Maker: An Automated System for Protein Purification<br />
Robin Clark, deCODE genetics<br />
Co-Authors: Alexandrina Muntianu, Hans-Thomas Richter, Denise Conner, Lawrence Chun,<br />
and Lance Stewart<br />
TP020 BD Biosciences Clontech ZsProSensor-1, a Fluorescent Protein-based Proteasome<br />
Sensor Assay: Evaluation Using the Acumen Explorer<br />
Matthew Cook, TTP LabTech<br />
Co-Authors: Olivier Dery, Gwyneth Olson, Annick Le Gall, Francine Fang, TTP LabTech; and<br />
Pierre Turpin, BD Bioscience Clontech<br />
TP021 Automated Isolation of Nucleic Acids on the Zymark SciClone ALH 3000 Using<br />
Promega’s SV 96 Nucleic Acid Isolation Chemistries<br />
Cristopher Cowan, Promega Corporation<br />
Co-Author: James Batchelor, Zymark Corporation<br />
TP022 Demonstration of Mitochondrial Aequorin Luminescent Signal Measurement<br />
by FLIPR3<br />
Carole Crittenden, Molecular Devices Corporation<br />
Co-Authors: Jennifer McKie and Yan Zhang<br />
TP023 Information and Data Management Software for Tracking Patient, Research, and<br />
Analysis Data in Small to Medium Clinical and Research Labs<br />
Katherine Dains, Adeline Scientific<br />
Co-Author: Wensheng Chen<br />
TP024 Novel PNA-Peptoid Conjugates as Antisense Drugs<br />
Juan Jose Diaz-Mochon, University of Southampton<br />
Co-Authors: Laurent Bialy, Boon-ek Yingyongnarongkul, Adam Belson, and Mark Bradley<br />
TP025 Developing a Versatile Program and Database for use in Laboratory Science<br />
(PhotochemCAD)<br />
James Dixon, North Carolina State University<br />
Co-Author: Jonathan S. Lindsey<br />
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TP026 In Silico Drug Profiling: QSAR Models as Frontline Weapons in the Fight to Find<br />
New Drug Candidates<br />
Doug Drake, IDBS<br />
Co-Authors: Andrew Lemon and Evgueni Kolossov<br />
TP027 Capability Management Framework for Clinical Equipment in Laboratory<br />
Philipp Dreiss, Fraunhofer IPA<br />
Co-Authors: R. Muckenhirn, J. Dorner, and A. P. Kumar<br />
TP028 SHOW – Sample Handling Operation Wizard<br />
Ping Du, Black Mountain Scientific<br />
TP029 Tools for Improving Purification Throughput for New Drug Candidates<br />
Wayne Duncan, Agilent Technologies<br />
Co-Author: Douglas McIntyre<br />
TP030 Automated Real Time Hit Picking, Retesting, and Reflux Testing<br />
Todd Edwards, Protedyne<br />
Co-Authors: Jason Quarles, Dave Wilson, Ralph K. Ito, and Gregory A. Endress<br />
TP031 XLC-MS: Sample Extraction and LC Separation Merged Into a Single Automated<br />
Front-end System<br />
Alex Berhitu, Spark Holland, Inc.<br />
Co-Authors: Steven Eendhuizen, Emile Koster, Martijn Hilhorst, and Bert Ooms<br />
TP032 Magnetic Bead Based Automated Isolation of Polyhistidine-Tagged Proteins for<br />
Purification and Target Screening<br />
Morten Egeberg, Dynal Biotech ASA<br />
Co-Authors: Ingrid Manger, Tommy Rivrud, Tine Borgen, Stine Bergholtz, and Dag Lillehaug<br />
TP033 High Throughput RNA Isolation - Methods Comparison<br />
Xingwang Fang, Ambion, Inc.<br />
Co-Authors: Roy C. Willis, Quoc Hoang, and Michael Siano<br />
TP034 ENCOMPASS: A New Expandable Approach for Large-Scale, Multi-Format<br />
Sample Management<br />
W. Steven Fillers, TekCel, Inc.<br />
TP035 Microfluidics Impact on Lab Aut<strong>omation</strong> and HTS<br />
Thomas Friedlander, Foster-Miller, Inc.<br />
TP036 Applications of a Highly Uniform UV Transillumination Imaging System for Quantitative<br />
DNA and Protein Analysis<br />
Sean Gallagher, UVP, Inc.<br />
Co-Authors: Alex Waluszko, Hui Zhang, John Wallace, and Kate Cole, UVP, Inc.; Molli<br />
Osburn, Scripps College<br />
TP037 Caco-2 Transport Assay Using New HTS Transwell ® 96-Well Permeable Supports<br />
Alice Gao, Corning Incorporated<br />
Co-Author: Debra Hoover<br />
TP038 Automated Aptamer Selection Against hnRNP A1 and its Proteolytic Derivative UP1<br />
Carlos Garcia, University of Texas at Austin<br />
Co-Authors: Andrew D. Ellington, J. Colin Cox, and Chi-Tai Chu<br />
TP039 Automated Selection of Aminoglycoside Antibiotic Aptamers<br />
Patrick Goertz, University of Texas at Austin<br />
Co-Authors: J. Colin Cox and Andrew D. Ellington<br />
29<br />
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POSTER PROGRAM LISTING
TP040 Mass Production of Plastic Chips for Microfluidic Applications<br />
Norbert Gottschlich, Greiner Bio-One, Inc.<br />
Co-Authors: A. Gerlach, G. Knebel, W. Hoffmann, A. E. Guber, and Forschungszentrum<br />
Karlsruhe, Institut für Mikrostrukturtechnik, Germany<br />
TP041 Anaylsis of Nucleic Acid and Protein Arrays on NUNC ArrayCote 16-Well Slides<br />
and 96-Well Plates<br />
Joseph Granchelli, NalgeNunc International<br />
Co-Authors: Dan Schroen and Tom Cummins<br />
TP042 Combining Lyophilisation and Centrifugal Evaporation to Make a Fast Drying Process<br />
That Results in Rapidly Resuspendable Dry Solid Compound<br />
David Griffin, Genevac, Inc.<br />
TP043 MagneSil ® Total RNA High Throughput Isolation<br />
Terri Grunst, Promega Corporation<br />
Co-Author: Dan Kephart<br />
TP044 Smart Nanodispensing – The Path to More Reliable Liquid Handling<br />
Carsten Haber, Seyonic SA<br />
Co-Author: Marc Boillat, Bart van der Schoot<br />
TP045 Plasmid DNA Purification Using the Eppendorf Perfectprep ® Plasmid 96 Vac Direct<br />
Bind Kit on the Eppendorf epMotion 5075 Workstation<br />
Jennifer Halcome, Eppendorf-5 Prime, Inc.<br />
Co-Authors: George Halley and Gerry Huitt<br />
TP046 The ELx405 HT 384-Well Microplate Washer: Designed to Meet the Rigors of<br />
Biomolecular Screening<br />
Paul Held, Bio-Tek Instruments<br />
Co-Author: Lenore Buehrer<br />
TP047 The Data Explosion: “Print-to-Database” Technology for the HTS Laboratory<br />
John Helfrich, NuGenesis Technologies<br />
TP048 Meeting the Needs of High Throughput Genetics-based Research<br />
Terry Hermann, LabVantage Solutions, Inc.<br />
Co-Authors: Terry Smallmon and J. Kelly Ganjei<br />
TP049 High Throughput Measurement of 41 Ca by Accelerator Mass Spectrometry to<br />
Quantitate Small Changes in Individual Human Bone Turnover Rates<br />
Darren Hillegonds, Lawrence Livermore National Laboratory<br />
Co-Authors: John S. Vogel, Lawrence Livermore National Laboratory; David Herold and Robert<br />
Fitzgerald, Veterans Affairs San Diego Healthcare System/University of California, San Diego<br />
TP050 Software Validation for Aut<strong>omation</strong> Projects<br />
Lynn Hilt, The Ashvins Group, Inc.<br />
Co-Author: Terry Weeks<br />
TP051 Ion Channel Assay Development Using Voltage Sensor Probes on the GENios Pro<br />
Multifunctional Reader From Tecan<br />
Randall Hoffman, Invitrogen Corporation<br />
Co-Author: Gerald Habenbacher, Tecan Austria<br />
TP052 Elevated Serum Total Protein as an Indicator of Chronic Viral Hepatitis<br />
and/or HIV Infection<br />
Dawn Marie Jacobson, Veterans Affairs San Diego Healthcare System<br />
Co-Author: David Herold<br />
TP053 Nano Crystal Hydride Stable DNA Probe<br />
Joong Hyun Kim, University of California, Riverside<br />
Co-Authors: Jared Stephens, Dimitrios Morikis, Mihrimah Ozkan<br />
30<br />
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TP054 Multi-Channel Dispenser for Micro-Array Printing<br />
Andreas Kuoni, University of Neuchatel<br />
Co-Authors: Marc Boillat, University of Neuchatel; Bart van der Schoot, Seyonic SA<br />
TP055 Data Mining and Visualization in Sports Medicine Aut<strong>omation</strong> by WebServer Oriented<br />
Software and WebBrowser Oriented User Interfaces<br />
Fred Lange, University of Rostock<br />
Co-Authors: Regina Stoll and Reinhard Vilbrandt<br />
TP056 Luminescence Assays and FDA CFR 21 Part 11 Compliance With the New<br />
LMax II384 Microplate Luminometer<br />
Joseph Machamer, Molecular Devices<br />
TP057 Monolithic Modules in Micro Total Analytical Systems Rapidly Fabricated From Plastics<br />
Dieudonne Mair, University of California, Berkeley<br />
Co-Authors: Timothy Stachowiak, Jean Frechet, and Emily Hilder, University of California,<br />
Berkeley; Frantisek Svec, Lawrence Berkeley National Laboratory<br />
TP058 The Design and Synthesis of High Affinity Ligands for Human Cyclophilin A<br />
Kirk Malone, The University of Edinburgh<br />
Co-Authors: Nicholas J. Turner and Malocolm Walkinshaw<br />
TP059 Rapid and Cost-Effective Prototyping of Plastic Microfabricated Devices for<br />
Mass Spectrometry<br />
Justin Mecomber, University of Cincinnati<br />
Co-Authors: Douglas Hurd and Patrick A. Limbach<br />
TP060 Automated Optimization of Aptamer Selection Buffer Conditions<br />
Gwendolyn M. Motz, The University of Texas<br />
Co-Authors: J. Colin Cox and Andrew D. Ellington<br />
TP061 Axiomatic Conceptual Design and Investigation of a New and Generic Laboratory<br />
Aut<strong>omation</strong> Robotic Workcell With Application to Proteomics<br />
Peyman Najmabadi, University of Toronto<br />
Co-Authors: Andrew A. Goldenberg and Andrew Emili<br />
TP062 Automated Proteomic Applications on Beckman Coulter’s Biomek ® NX Laboratory<br />
Aut<strong>omation</strong> Workstation<br />
Laura Pajak, Beckman Coulter, Inc.<br />
Co-Authors: Chad Pittman and Scott Boyer<br />
TP063 Aut<strong>omation</strong> of Immunotech’s IL-8 ELISA Using Beckman Coulter’s<br />
Assay WorkStation Version 1.5<br />
Chad Pittman, Beckman Coulter, Inc.<br />
Co-Authors: Laura Pajak and Scott Boyer<br />
TP064 Using the Analytical Information Markup Language (AnIML) to Represent<br />
LC-Diode-Array Data<br />
Dominik Poetz, National Institute of Standards and Technology<br />
TP065 Poly-Ethylene-Glycol Grafted Non-fouling Nanoporous Alumina Membranes<br />
Ketul C. Popat, Boston University<br />
Co-Authors: Tejal A. Desai and Gopal Mor, Boston University; Craig Grimes, Pennsylvania<br />
State University<br />
TP066 Aut<strong>omation</strong> of In Situ Hybridization on Tecan HS Series Hybridization Stations<br />
Johannes Posch, Tecan Austria GmbH<br />
Co-Authors: G. Probst, K. Kratochwil, H. Bauer, R. Fuchs and G. Kreil, Tecan Austria GmbH;<br />
M. Köprunner, Austrian Academy of Science, Institute of Molecular Biology<br />
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POSTER PROGRAM LISTING
TP067 Neurons as Sensors: Mixed Chemical Agent Sensing<br />
Shalini Prasad, University of California, Riverside<br />
Co-Authors: Xuan Zhang, Mo Yang, Cengiz Ozkan, and Mihri Ozkan<br />
TP068 Evaluating and Determining Precision and Accuracy for Automated Liquid Handlers<br />
Dispensing Nanoliter Volumes of Viscous Solutions<br />
Kirby Reed, Gilson, Inc.<br />
TP069 NanoLC-MS Analysis of TNFα in Microdialysates<br />
Laurent Rieux, University of Groningen<br />
Co-Authors: Patty Mulder, Harm Niederlaender, Elisabeth Verpoorte, and Rainer Bischoff<br />
TP070 The Enhancement of Selectivity of a Pd-catalysed Three-component Cascade<br />
Reaction Using Automated Parallel Synthesis and Multivariate Data Analysis<br />
Kate Ross, University of Leeds<br />
Co-Author: Ronald Grigg<br />
TP071 Robustness and Flexibility for Scheduling<br />
Stephan Rudlof, Fachhochschule Wiesbaden - University of Applied Sciences<br />
TP072 Creating the Analytical Information Markup Language (AnIML)<br />
Burkhard Schaefer, National Institute of Standards and Technology<br />
Co-Author: Gary Kramer<br />
TP073 Rapid and Sensitive Determination of Cardiovascular Drugs in Mouse Plasma Using<br />
Solid-phase Extraction and RP-HPLC<br />
Sadhana Sharma, The Ohio State University<br />
Co-Authors: John Shapiro, Stephen C. Lee, and Mauro Ferrari<br />
TP074 Automated Double-stranded DNA Aptamer Selections<br />
Letha Sooter, The University of Texas at Austin<br />
Co-Author: Andrew Ellington<br />
TP075 Fabrication of a Polymer Based Bio-sensing Optical Component<br />
Henrik Schiøtt Sørensen, Risø National Laboratorium<br />
Co-Authors: Niels B. Larsen and Peter E. Andersen, Risø National Laboratorium; Darryl J.<br />
Bornhop, Vanderbilt University<br />
TP076 A Generic Model for Timely Refreshing of Very Large Semi-Dynamic Web Sites<br />
Duraisamy Sridharan, Anna University<br />
Co-Authors: P. Sakthivel and S. K. Srivatsa<br />
TP077 Automated Process of Q-PCR/Gene Expression<br />
Claudia Stewart, NCI-FCRDC<br />
Co-Authors: James M. Cherry, Kelly T. Martin, Naryan K. Bhat, and David Munroe<br />
TP078 Fuzzy Based Medical Expert System for Automated Data Interpretation in<br />
Occupational Medicine<br />
Regina Stoll, University of Rostock<br />
Co-Authors: Mohit Kumar and Norbert Stoll<br />
TP079 An Automated, High Throughput Cell-Based Assay System Using the Biomek 3000<br />
Laboratory Aut<strong>omation</strong> Workstation and CellProbe HT Caspase 3/7 Whole Cell Assay<br />
Yu Suen, Beckman Coulter, Inc.<br />
Co-Authors: Keith Roby, Javorka Gunic, Michael H. Simonian, and Graham Threadgill<br />
TP080 Polymeric Microdevices for Applications in Targeted Drug Delivery<br />
Sarah Tao, Boston University<br />
Co-Authors: Ka Wah Lee and Tejal Desai, Boston University; Mike Lubeley, University of<br />
Illinois, Chicago<br />
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TP081 A High Throughput Replacement for Western Blots: Protein Expression Analysis<br />
Using the MSD Multi-Array Platform<br />
Robert Umek, Meso Scale Discovery<br />
Co-Authors: Lisa Gazzillo, Anu Mathew, Malcolm Smith, Timothy Schaefer, and<br />
Jacob N. Wohlstadter<br />
TP082 Micro Parallel Liquid Chromatography for High Throughput ADMET Profiling<br />
Surekha Vajjhala, Nanostream, Inc.<br />
Co-Authors: Li Zhang, Paren Patel, Jeffrey Koehler, Steve Hobbs, and Chris Phillips<br />
TP083 Cell Based Assays in 384- and 1536-Well Formats using MosQuito and the<br />
Acumen Explorer<br />
Ian Whitehall, TTP LabTech Ltd<br />
Co-Authors: Paul Wylie, Rob Lewis, and Wayne Bowen<br />
TP084 A High Throughput Microfluidic Protease Substrate Identification Assay<br />
Hayley Wu, Caliper Technologies Corp.<br />
Co-Authors: Javier Farinas, Charles Park, Cheryl Cathey, Christopher Tsu, and Michael<br />
Pantoliano, Caliper Technologies Corp.; Lawrence Dick, Millennium Pharmaceuticals<br />
TP085 On Chip Chemical Assay by Forming Droplets in Fluidic Systems<br />
Hongkai Wu, Stanford University<br />
Co-Author: Richard N. Zare<br />
TP086 Application of Holliday Junction Based Allele-Specific (HAS) Genotyping Platform for<br />
Detecting Two Common Thrombophilia Genetic Mutations<br />
Wendy Yang, FreshGene, Inc.<br />
Co-Authors: Qinghong Yang, Sandra Hatcher, and Henrietta Seet, FreshGene, Inc.;<br />
Jeffrey Gregg, University of California, Davis – Medical Center<br />
TP087 Automated Strategies For Protein Precipitation Filtration And Solid Phase Extraction<br />
(SPE) Optimization On the TECAN Robotic Sample Processor – Applications In<br />
Quantitative LC-MS/MS Bioanalysis<br />
Lilly Zhang, Amgen, Inc.<br />
Co-Authors: John D. Laycock, Jill Hayos, Julie Flynn, Gary Yesionek, and Krys J. Miller<br />
TP088 The OroTherm Model HC1080, Orochem Heating and Cooling Plate – A Unique<br />
Instrument Using the Latest Technology in Thermoelectric Cooling (TEC)<br />
Jaskiran Kaur, Orochem Technologies, Inc.<br />
Co-Author: Asha Oroskar<br />
TP089 Aut<strong>omation</strong> in Monsanto Biotechnology<br />
Steve Dulle, Monsanto<br />
Co-Authors: Renee Matlick, Gina Balch, Thomas Le, Mary M. Blanchard, Mark Vaudin<br />
TP090 Development of an Automated Workcell Around a Multicapillary Zone Electrophoresis<br />
Instument for Human Serum Protein Analysis<br />
Alain Truchard, Center of Research in Biomedical Technology<br />
Co-Authors: D. Morin, F. X. Chobletf, S. Belsoeur, T. Le Neel, Center of Research in<br />
Biomedical Technology, P. Chauvin, Sebia<br />
TP091 Aut<strong>omation</strong> Systems for Bioscreening – Development and Services<br />
Kerstin Thurow, University of Rostock<br />
Co-Author: Kristin Entzian<br />
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POSTER PROGRAM LISTING
These posters should be put up on Wednesday by 10:30 am and removed by 6:30 pm on<br />
Wednesday. Authors must be present 1:30 – 3:00 pm on Wednesday.<br />
WP001 Automating Large DNA Insert Preparation for Sequencing<br />
Chris Barbagallo, Millipore Corporation<br />
Co-Authors: Masaharu Mabuchi, Janet Smith, Peter Rapiejko, and Joseph Hitti<br />
WP002 XLC-MS for Therapeutic Drug Monitoring<br />
Steven Eendhuizen, Spark Holland, Inc.<br />
Co-Authors: Alex Berhitu, Emile Koster, Peter Ringeling, Bert Ooms<br />
WP003 Use of the AcroPrep 96 Filter Plate for the Parallel Preparation of Recombinant Proteins<br />
Emily Berlin, Pall Life Sciences<br />
Co-Authors: Tao Hu and Kevin Seeley<br />
WP004 Neutrophil Adhesion: A HTS Compatible Assay Using the Acumen Explorer<br />
Wayne Bowen, TTP LabTech<br />
Co-Author: John Budd<br />
WP005 Oxidative Burst: Amplex ® Red Detection of Hydrogen Peroxide Release From<br />
Differentiated HL-60 Cells Using FlexStation II384 Microplate Reader and the<br />
HTS Format of FLIPR3<br />
Carole Crittenden, Molecular Devices Corporation<br />
Co-Authors: Jennifer McKie and Yan Zhang<br />
WP006 Making Sense of it All: Data Management for the New Challenges in Drug Discovery<br />
Doug Drake, IDBS<br />
Co-Authors: Michael Collingsworth and Jack Elands<br />
WP007 Magnetic Bead Based High Throughput PCR- and Sequence Cleanup<br />
Morten Egeberg, Dynal Biotech ASA<br />
Co-Authors: Tommy Rivrud, Erling Finne, Dag Lillehaug<br />
WP008 Guidlines for Selecting XYZ Machines for High Precision High Throughput<br />
Lab Aut<strong>omation</strong><br />
Boaz Eidelberg, Bayside Motion Group<br />
Co-Author: Joe Timpone<br />
WP009 Streamlining Aut<strong>omation</strong> of In-Gel Digestion and MALDI Target Spotting<br />
Marcy Engelstein, Millipore Corporation<br />
Co-Authors: Libby Kellard and Anja Dedeo, Millipore Corporation; Mikkel Nissum, Tecan<br />
WP010 High Throughput Sample Preparation for RNAi Studies and Expression Profiling<br />
Xingwang Fang, Ambion, Inc.<br />
Co-Authors: Roy C. Willis, Quoc Hoang, Michael Siano, Weiwei Xu<br />
WP011 Performance of 384 Low Volume Microplates in FP-based GPCR Binding Assays<br />
Alice Gao, Corning Incorporated<br />
Co-Author: Debra Hoover<br />
WP012 Information and Image Management for Proteomics-based Research<br />
Terry Hermann, LabVantage Solutions, Inc.<br />
Co-Authors: J. Kelly Ganjei, Shariq Alavi<br />
WP013 Low Birefringence Plates for Crystal Scoring Under Polarized Light<br />
Günther Knebel, Greiner Bio-One, Inc.<br />
Co-Author: Ulrike Honisch<br />
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WP014 New High Performance Magnetic Separation Technology for Laboratory and<br />
Industrial Applications<br />
David Humphries, Lawrence Berkeley National Laboratory<br />
Co-Authors: Martin Pollard and Chris Elkin, Lawrence Livermore National Laboratory<br />
WP015 Pfizer Global Research and Development Material Management Global Center of<br />
Emphasis: Global Liquid Operations at the Groton Kings Heights Technology Center<br />
Diane Johnson, Pfizer Global Development and Research<br />
Co-Authors: Steve Brinkman, William Heineman, Craig Hines, Michele Kelly, Kim Matus<br />
WP016 Automated DNA Sequencing: Quality Not Quantity<br />
Mike Jones, Cambridge Antibody Technology<br />
Co-Authors: Richard Stevens, Kate Goode<br />
WP017 Aut<strong>omation</strong> of BAC 96 DNA Purification<br />
Lynn Jordan, Zymark Corporation<br />
Co-Authors: Jim Batchelor, Kelly M. Clark, and Joseph A. Hensley, Brinkmann – An<br />
Eppendorf Company; Jennifer L. Halcome and George R. Halley, Eppendorf – 5 Prime, Inc.<br />
WP018 Improving the Performance of Mass Spectrometry Analysis With Automated<br />
Peak-Parking Using Off-the-shelf Components<br />
Sanjaya Joshi, Userspace Corporation<br />
Co-Authors: David R. Goodlett and Hookeun Lee, Institute for Systems Biology<br />
WP019 High Throughput Protein Recovery From Organic Solvents Using AcroPrep 96<br />
Multi-well Plates Containing Mustang Ion Exchange Membranes<br />
Jeff Kane, Pall Life Sciences<br />
Co-Authors: Kevin Seeley, Emily Berlin<br />
WP020 Aut<strong>omation</strong> of Receptor-Ligand Binding Assays Using the MultiScreen ® HTS Filter Plate<br />
Libby Kellard, Millipore Corporation<br />
Co-Authors, Sonia Gil, Steven D. Sheridan<br />
WP021 Automated Genomic DNA Purification From Large Volumes of Whole Blood<br />
Dan Kephart, Promega Corporation<br />
Co-Authors: Cris Cowan, Terri Grunst<br />
WP022 Multiplex Measurements of Cytokines in High Density Formats Using<br />
Multi-Array Technology<br />
Alan Kishbaugh, Meso Scale Discovery<br />
Co-Authors: Gisbert Spieles, Erin Grossi, Kent Johnson, Rob Calamunci, George Sigal,<br />
Svetlana Leytner, Jacob N. Wohlstadter<br />
WP023 An Automated, Portable Immunochemical Flow Injection System for On-Site Analysis<br />
of Environmentally Hazardous Chemicals<br />
Gunther Kolb, Institut für Mikrotechnik Mainz GmbH<br />
Co-Authors: Ines Frese, Volker Hessel, Holger Löwe, David Tiemann, and Ioan M.<br />
Ciumasu, Institut für Mikrotechnik Mainz GmbH; Petra M. Krämer, TU München,<br />
Wissenschaftszentrum Weihenstephan<br />
WP024 Multiple Parallel Purification of Fab Fragments Discovered Using Dyax Corp’s Phage<br />
Display Antibody Libraries<br />
Kristopher Kopacz, Dyax Corporation<br />
Co-Authors: Qi-Long Wu, Janja Cosic, David Buckler<br />
WP025 Identifying and Reducing Crossover Contamination at the Sub-Microliter Level for<br />
Pipetting Tips in a High Throughput DNA Sequencing Environment<br />
Duane Kubischta, DOE Joint Genome Institute<br />
35<br />
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POSTER PROGRAM LISTING
WP026 Fully-Automated, High Throughput Peptide Mass Fingerprinting by MALDI<br />
Orthogonal-TOF Mass Spectrometry<br />
Scott Kuzdzal, PerkinElmerSCIEX<br />
Co-Authors: Joe DiCesare, Mary Lopez, Lisa Sapp, Tillmann Ziegert<br />
WP027 KingFisher 96 – A Novel High Throughput Platform for Protein Applications<br />
Arja Lamberg, Thermo Electron<br />
Co-Authors: Merja Mehto and Arja Lamberg, Thermo Electron; Stine Bergholtz;<br />
Tine Borgen, David Gillooly, and Diem Tran, Dynal Biotech; Virpi Kymäläinen and<br />
Maija Partanen, Thermo Labsystems<br />
WP028 High Resolution Mobile Measurement of Oxygen Concentration in Main Breath Stream<br />
by Optical Oxygen Sensing – An Application in Occupational and Sports Medicine<br />
Fred Lange, University of Rostock<br />
Co-Authors: Regina Stoll, Thomas Renger<br />
WP030 The HTS Compound-Thawing Bottle Neck (and How to Avoid it With a New Processor)<br />
Kurt Lund, ACESystems, Inc.<br />
WP031 Recent Developments in High Throughput, Turn-Key Solubility, and Permeability<br />
Compound Ranking Assays<br />
Joseph Machamer, Molecular Devices<br />
Co-Authors: Greg Kazan, Molecular Devices; Tom Onofrey, Millipore Corp.<br />
WP032 High Throughput Process Optimization for the Fine Chemical and<br />
Pharmaceutical Industries<br />
Colin Masui, Symyx Technologies<br />
WP033 IQ ® Technology: An Automated HTS Assay for Screening Kinase, Phosphatase, and<br />
Protease Targets<br />
Timothy McCauley, Pierce Biotechnology, Inc.<br />
Co-Authors: Mahesh Mathrubutham, Sherri Z. Millis, Aric G. Morgan, Michael L. Stanaitis<br />
WP034 Solving the Dispensing Challenges of Assay Miniaturization in High-Density Microplates<br />
Ruth H Myers, Aurora Instruments, LLC<br />
Co-Authors: Jason Johnson, Chris Biagioli<br />
WP035 Ultra-High Throughput Screening Using Multi-Array 1536-Well Plates<br />
Pankaj Oberoi, Meso Scale Discovery<br />
Co-Authors: David Stewart, Kevin Khovananth, Stephen Tessier, Alan Kishbaugh, Kent<br />
Johnson, Jacob Wohlstadter<br />
WP036 Membrane-Bound Protein Crystallization Workstation<br />
Jeff Olson, Abbott Laboratories<br />
Co-Authors: Mark Chiu, Mike McCoy, Jeff Pan<br />
WP037 Cell Based Biosensors<br />
Cengiz S. Ozkan, University of California, Riverside<br />
WP038 Using Beckman Coulter’s Biomek ® NX Laboratory Aut<strong>omation</strong> Workstation for the<br />
Purification of High Quality Plasmid DNA<br />
Laura Pajak, Beckman Coulter, Inc.<br />
Co-Authors: Chad Pittman, Scott Boyer<br />
WP039 Right Time, Right Place – Right Information?<br />
Geoff Parker, Scimcom<br />
WP040 Aut<strong>omation</strong> Platform for Salt Prescreening and Polymorph Screening Studies<br />
Fiona Payne, Zinsser Analytic GmbH<br />
Co-Author: Werner Zinsser<br />
36<br />
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WP041 AquaMax DW4: A Flexible Tool for Heterogeneous 1536 Assays<br />
Jonathan Petersen, Molecular Devices Corp.<br />
Co-Authors: Jeannie Nguyen, Annegret Boge, Gayle Teixeira<br />
WP042 Aut<strong>omation</strong> of Immunotech’s IL-8 ELISA Using Beckman Coulter’s Biomek 3000<br />
Laboratory Aut<strong>omation</strong> Workstation<br />
Chad Pittman, Beckman Coulter, Inc.<br />
Co-Authors: Laura Pajak, Scott Boyer<br />
WP043 STEM ReactArray Improved Productivity Using Manual and Automated Parallel<br />
Reactors Equipped With Liquid Handling and On-line HPLC Analysis<br />
Ileana Place, STEM/ReactArray<br />
WP044 Characterization of Human Alpha-Thrombin Aptamer System by Automated<br />
Fluorescence Lifetime Analysis<br />
Dieter Popp, Tecan Austria GmbH<br />
Co-Authors: Mateja Niederreiter, Manfred Lansing, David Yost, Klaus Döring, Melissa<br />
Foshee<br />
WP045 Tecan’s LSx00: Automated Processing and Analysis of Microarrays Implemented Prior<br />
Content Quality Control<br />
Johannes Posch, Tecan Austria GmbH<br />
Co-Authors: Ralph Beneke, Gerald Probst<br />
WP046 High Throughput Peptide Synthesis on a Biomek FX Liquidhandler<br />
Lynn Rasmussen, SAIC: NCI Frederick<br />
Co-Authors: Carl Saxinger, Casey Frankenberger, David Munroe, SAIC: NCI Frederick;<br />
Marc Goldstein, Beckman-Coulter<br />
WP047 Novel Approach for Screening of Drug Absorption Via an Automated System<br />
Kirby Reed, Gilson, Inc.<br />
Co-Author: Alan Hamstra<br />
WP048 Tools for Sample Management that are Efficient, Versatile, Proven and Evolving –<br />
REMP Tube Technologies<br />
Scott Reeves, REMP USA<br />
Co-Authors: Michael Girardi, REMP USA; Carol Homon, Boehringer Ingelheim<br />
Pharmaceuticals, Inc; Thorsten Poetter, Bayer Crop Science AG; Berta Strulovici, Merck &<br />
Company, Inc.; Gerhard Mihm, Boehringer-Ingelheim Pharma KG<br />
WP049 Multichannel HTS/ µHTS Liquid Handling Systems – Check Using<br />
Two-Indicator Systems<br />
Heidrun Rhode; Friedrich-Schiller-University, Medical Faculty<br />
Co-Authors: M. Schulze, G. A. Cumme, A. Horn, Simon Renard, and Thomas Moore,<br />
Friedrich-Schiller-University, Medical Faculty; Peter Zimmermann, CyBio AG<br />
WP050 AliQuot – High Throughput Liquid Dispensing Into Microplates<br />
Steve Richmond, Genetix Ltd<br />
Co-Authors: Sky Jiang, Paul Raine, Sarah Stephens, Chris Mann, Julian F. Burke<br />
WP051 A Totally Automated Solution for Normal and RP Preparative HPLC With Analytical<br />
Purification Determination: cLC<br />
Luke Roenneburg, Gilson, Inc.<br />
Co-Author: Alan Hamstra<br />
WP052 A Homogenous Assay for Inhibition of Basal Proliferation in 96- and 384-Well Formats<br />
Using the Acumen Explorer<br />
Jas Sanghera, TTP LabTech<br />
Co-Authors: David Pole, Wayne Bowen<br />
37<br />
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POSTER PROGRAM LISTING
WP054 Innovations in Photonics for Biotech Applications<br />
Eric Schmidt, Ahura Corporation<br />
Co-Author: Daryoosh Vakhshoori<br />
WP055 First Dual Resolution Syringe (DRS), Using Differential Displacement, for<br />
Practical Contact-Free Nanoliter Transfer of Discrete Samples or Within-tip Mixtures,<br />
and Extreme Dilutions<br />
Donald Schwartz, DRD<br />
Co-Author: Donald S. Martin<br />
WP056 Scaling From 96- to 384-Well Assay Platforms: Characterization of Receptor-Ligand<br />
Binding Using 384-Well Filter Plates Optimized For Maximum Radiation Detection<br />
Steven Sheridan, Millipore<br />
Co-Authors: Sonia Gil, Libbey Kellard<br />
WP057 SpectraMax M2 Multi-detection System Allows Validated Microplate-based<br />
Fluorescence and Absorbance Assays in a GLP/GMP Environment<br />
Christopher Silva, Molecular Devices Corporation<br />
WP058 Using the Biomek ® 3000 Laboratory Aut<strong>omation</strong> Workstation to Interface 2D Liquid<br />
Chromatography With Mass Spectrometry for Multidimensional Proteome Profiling<br />
Michael Simonian, Beckman Coulter, Inc.<br />
Co-Authors: Matthew Cu, Edna Betgovargez, Graham Threadgill<br />
WP059 Automated DELFIA ® Filtration Assays in 96- and 384-well Format for GPCR Studies<br />
Katja Sippola, PerkinElmer Life and Analytical Sciences, Wallac Oy<br />
Co-Authors: Joni Helenius, Sanna Rönnmark, Maija-Liisa Mäkinen, Jari Suontausta, Christel<br />
Gripenberg-Lerche, Satu Kovanen<br />
WP060 FIZICS: A Novel Macro Imaging System<br />
Stephen Skwish, Merck & Company, Inc.<br />
Co-Authors: Francisco Asensio, Greg King, Glenn Clarke, Gary Kath, Michael J. Salvatore,<br />
Claude Dufresne<br />
WP061 Aut<strong>omation</strong> Systems Run in Parallel<br />
Ginger Smith, GlaxoSmithKline<br />
Co-Authors: Amy Siu, Renae Crosby, Jim Liacos, Cathy Finlay, Jimmy Bruner<br />
WP062 Automated Sampling System for Parallel Autoclaves in High Throughput Chemistry<br />
Norbert Stoll, University of Rostock<br />
Co-Authors: Wof-Dieter Heinitz, Hans-Joachim Stiller, KerstinThurow<br />
WP063 Real-time Physical Fitness Validation by Automated Respiratory Analysis<br />
Regina Stoll, University of Rostock<br />
Co-Author: Norbert Stoll<br />
WP064 96-Well Caco-2 transport Model for Drug Permeability Studies<br />
Rowan Stringer, Novartis Horsham Research Centre<br />
Co-Authors: Elizabeth Willmott, Rachael Profit, Sarah Beech, Paul Nicklin<br />
WP065 Dual-Glo Luciferase Assay Measurements in the LMax II 384 Microplate<br />
Luminometer and the Analyst ® GT Multimode Reader<br />
Michael Su, Molecular Devices Corporation<br />
Co-Authors: Jinfang Liao, Evelyn McGown<br />
WP066 Aut<strong>omation</strong> of Caco-2 Cell Preparation, Drug Permeation and Transport Assay on the<br />
Biomek 3000 Laboratory Aut<strong>omation</strong> Workstation<br />
Yu Suen, Beckman Coulter, Inc.<br />
Co-Authors: Keith Roby, Javorka Gunic, Michael H. Simonian<br />
38<br />
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WP067 Application of Aut<strong>omation</strong> for Clinical Newborn Screening: Detection of Hearing Loss<br />
Associated Cytomegalovirus<br />
Joseph Suzow, Pediatrix Screening<br />
Co-Authors: Zhili Lin, Michelle Lage, Edwin W. Naylor<br />
WP068 Application of High Throughput Screening for Pre-formulations Using the Symyx<br />
Technologies Workflow<br />
Anny Tangkilisan, Symyx Technologies<br />
WP069 Automated Screening of Cytotoxic Compounds<br />
Kerstin Thurow, University of Rostock<br />
Co-Author: Kristin Entzian<br />
WP070 Integrating Software Validation Throughout Software Development<br />
Melissa Trout, Code Refinery<br />
Co-Authors: Samir Dandekar, Mike Brown<br />
WP071 High Throughput Multiplexed Assays for Biomarkers and Phosphoproteins<br />
Robert Umek, Meso Scale Discovery<br />
Co-Authors: Paula Denney Eason, Jenny Ly, Jacob N. Wohlstadter<br />
WP072 Rapid Compound Purity Screening using the Nanostream Veloce System<br />
Surekha Vajjhala, Nanostream, Inc.<br />
Co-Authors: Li Zhang, Paren Patel, Sergey Osechinskiy<br />
WP073 SearchLight Proteome Arrays: Multiplexed Assays for High Content Screening<br />
Scott Van Arsdell, Pierce Biotechnology, Inc.<br />
Co-Authors: Rajiv Pande, Christine Burns<br />
WP074 A Cell-Based Multi-label DELFIA Assay for Measuring Phosphorylation of Proteins<br />
Sofia Vikstrom, PerkinElmer Life and Analytical Sciences<br />
Co-Authors: Christel Gripenberg-Lerche, Pertti Hurskainen, Ilkka Hemmilä<br />
WP075 SynCar: A New Automated Solution Phase Synthesis Platform for Medicinal Chemistry<br />
Erich von Roedern, Aventis Pharma<br />
Co-Authors: Angelika Weber, Hans-Ulrich Stilz<br />
WP076 Aut<strong>omation</strong> Tools To Aide Information Management in a Pharmaceutical<br />
Discovery Environment<br />
Wei Wang, Pfizer<br />
Co-Authors: Brian Boyd, Neelesh Nundkumar, Mark Proefke<br />
WP078 Introduction of Aut<strong>omation</strong> Into Clinical Laboratories in the UK<br />
Mike Wheeler, Guy’s and St Thomas’ Hospital Trust<br />
Co-Authors: Carolyn Piggott, Guy’s and St. Thomas’ Hospital Trust; Stephen Halloran,<br />
University of Surrey<br />
WP079 Primary HTS Neurite Outgrowth Assay Using the Acumen Explorer<br />
Ian Whitehall, TTP LabTech Ltd<br />
Co-Authors: John Budd, Paul Wylie, Wayne Bowen<br />
WP080 High Throughput Viral RNA Isolation from Swab, Serum and Plasma<br />
Chris Willis, Ambion, Inc.<br />
Co-Authors: Xingwang Fang, Michael A Siano<br />
WP081 Integrating Microsoft’s .NET Platform Into the DOE’s Production Genomics Facility<br />
Steven Wilson, JGI<br />
WP082 Microfluidic Kinase Selectivity Screening Assays in Off-Chip Assay Format<br />
Hayley Wu, Caliper Technologies Corp.<br />
Co-Authors: Holly Reardon, Thi Ngoc Vy-Trinh, Ella Li, Javier Farinas, Jude Dunne<br />
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POSTER PROGRAM LISTING
WP083 Development of an Automated High Throughput Screening Enzyme Linked<br />
Immunosorbent Assay for Chronic Wasting Disease Prions in Elk and Deer<br />
John T. Y. Wu, Government of Alberta<br />
Co-Authors: Eva Y. W. Chow, Lester S. Y. Wong, Evelyn E. Bowlby<br />
WP084 Fast Spin Centrifuge, Analytical Module and Automated Laboratory System<br />
Michael Yavilevich, Inventor<br />
WP085 Quantitative Biological Sample Analysis Using NanoESI (Nanomate 100 ® ) –MS/MS<br />
Lilly Zhang, Amgen, Inc.<br />
Co-Authors: John D. Laycock, Krys J. Miller<br />
WP086 Plasmid Purification Using Promega’s Wizard* SV96 Reagents and Beckman Coulter’s<br />
Biomek ® 3000 Laboratory Aut<strong>omation</strong> Workstation<br />
Ruth Zhang, Beckman Coulter, Inc.<br />
Co-Authors: Chad Pittman, Scott Boyer, Laura Pajak<br />
WP087 Return of the Centrifuge: Automated DNA Extraction by Small Production Islands<br />
Juergen Zimmermann, EMBL<br />
Co-Authors: Vladimir Benes, Christian Boulin, and Ralf Griebel, EMBL; Paul Lomax, Perkin<br />
Elmer Life Sciences; Thomas Zinn, Ullrich Schübel, Macherey Nagel, and Klaus Günther<br />
Eberle, Hettich GmbH & Co KG<br />
40<br />
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PODIUM ABSTRACTS<br />
Plenary Sessions – Page 43<br />
High Throughput Chemistry – Page 45<br />
ADME Toxicology – Page 55<br />
Drug Discovery Case Studies – Page 56<br />
High Throughput Screening – Page 58<br />
Microfluidics – Page 68<br />
Proteomics – Page 82<br />
Aut<strong>omation</strong> Applications in Process R&D – Page 93<br />
Genomics – Page 95<br />
Clinical – Page 108<br />
Engineering Applications – Page 122<br />
Emerging Technologies – Page 124<br />
National Laboratory Technology Transfer – Page 136<br />
Emerging Informatics – Page 139<br />
Performance Metrics – Page 141<br />
41<br />
PODIUM ABSTRACTS
NOTES<br />
42
8:30 am Tuesday, February 3 Opening Plenary Session Room A2<br />
Jürgen Drews<br />
Nigeons GmbH<br />
Firnhaberstrasse 14<br />
Feldafing, D-82340 Germany<br />
drews@nigeons.com<br />
Changing Patterns in the Discovery of New Drugs<br />
The promise of genomics in drug discovery, which was so eagerly embraced in the mid-1990s, has not yet been<br />
fulfilled. There are two main reasons for this initial failure: The first one has to do with the fact that the structures<br />
and functions of gene products, which were included in screening programs, were poorly understood. Secondly,<br />
the combinatorial libraries, to which these “targets” were exposed were lacking in quality. As a result of these<br />
shortcomings, the overall productivity of the drug industry has declined further. However, this statement needs<br />
differentiation because the weights within the drug industry have shifted. The first tier biotech companies have<br />
now become the most productive segment of the drug industry. They often combine a high degree of innovative<br />
spirit with solid pharmaceutical professionalism. Some smaller biotech firms have succeeded in addressing<br />
unmet medical needs in technologically appealing ways. Many well positioned larger biotech companies with rich<br />
development portfolios suffer from development times that are longer than expected, costs that are higher than<br />
expected and from failures in clinical trials that were not expected at all. The classical pharmaceutical industry<br />
continues on its path to further consolidation with a clear emphasis on marketing and sales. By and large, the<br />
big pharmaceutical companies take a cautious attitude towards novel science and technology. A convincing<br />
balance between the need for good-looking bottom lines and a compelling long-term vision has not emerged. In<br />
their totality these changes have deeply altered the nature and the appearance of the drug industry. In the mid-<br />
to long-term, however, science and technology offer great opportunities to overcome this stagnation. Biology,<br />
especially biological approaches that are directed at the functioning of systems rather than on single molecular<br />
entities will eventually solve the problem of identification and validation of suitable drug targets. The continued<br />
study of drug protein interactions at the molecular level is built on biological premises but is chemical in nature.<br />
We begin to understand that the majority of pharmaceutically relevant drug targets cluster into densely populated<br />
target families, thus offering a novel chemical approach. This new strategy uses a privileged structure concept<br />
whereby molecular master keys are developed that take into account target family wide structural and functional<br />
commonalities. Lead compounds can be generated that address a variety of targets from a particular gene family<br />
that may reach across the boundaries of therapeutic areas. Optimizing a master key for a distinct target family,<br />
therefore, is about to become a new chemical strategy, which will replace strategies of the recent past which<br />
were built on large nu<strong>mbers</strong> of compounds that were not chosen on the basis of a postulated or demonstrated<br />
relationship of target and ligand. In concert, these new approaches may well improve the productivity of drug<br />
research and again alter the landscape of the respective industry.<br />
9:15 am Tuesday, February 3 Opening Plenary Session Room A2<br />
John D. Rhodes<br />
Deloitte & Touche<br />
Two Hilton Court<br />
P.O. Box 319<br />
Parsippany, New Jersey 07054-0319<br />
jorhodes@deloitte.com<br />
Financial Forces Shaping the Future of the Pharmaceuticals and Biotech Industry<br />
Deloitte’s National Managing Partner, Life Sciences, will provide a view of the current life sciences landscape and<br />
discuss key trends and issues impacting the industry. Key industry metrics will be reviewed including global sales<br />
trends, FDA approval rates, level of R&D expenditures and investment trends in forming alliances and partnerships.<br />
The discussion will also highlight challenges impacting future growth rates including the state of regulatory<br />
challenges, innovation and development efforts as well as protection of intellectual property.<br />
43<br />
PODIUM ABSTRACTS
10:30 am Tuesday, February 3 Plenary Session: A Look to the Future Room A2<br />
Richard A. Mathies<br />
University of California, Berkeley<br />
307 Lewis Hall<br />
Berkeley, California 94720<br />
rich@zinc.cchem.berkeley.edu<br />
Microfluidics in Your Future<br />
Microfabricated microfluidic chemical and biochemical analysis systems have advanced tremendously since their<br />
introduction in the early 1990’s. High-density and high throughput analyses are now routinely performed with<br />
arrays of microreactors, microarrays and microfabricated capillary electrophoresis channels. The full exploitation<br />
of these analysis capabilities awaits the development of nanoliter microfluidic sample preparation and handling<br />
capabilities that are integrated with the high throughput analyzer. For example, one recent publication explored<br />
the feasibility of integrating the entire shot-gun sequencing process on a wafer. If analogous microdevice systems<br />
could be developed that fully integrate the sample preparation and analysis processes in, for example, sequencing,<br />
genotyping, infectious disease detection, forensic identification, pathogen detection, and environmental monitoring,<br />
they would drive a paradigm shift in high throughput clinical and research labs. The development and application<br />
of such fully integrated chemical and biochemical “microprocessor” will also significantly impact point-of-care and<br />
point-of-analysis.<br />
11:15 am Tuesday, February 3 Plenary Session Room A2<br />
Christopher R. Lowe<br />
University of Cambridge<br />
Institute of Biotechnology<br />
Tennis Court Road<br />
Cambridge, CB2 1QT United Kingdom<br />
c.lowe@biotech.cam.ac.uk<br />
The Nanophysiometer: Holographic Sensors for Drug Discovery<br />
44<br />
Co-Author(s)<br />
Jeff Blyth, Alex Marshall, Anthony James,<br />
Satyamoorthy Kabilan, Felicity Sartain,<br />
Mei-Ching Lee, Blanca Madrigal-Gonzalez,<br />
Xiao-Ping Yang, Colin Davidson<br />
This session will address many of the current needs in pharmaceutical discovery and promises timely and<br />
economical solutions. There is a desire to move “high content” screening into a “high throughput” mode to<br />
accelerate the drug discovery pipeline. In effect, this would collapse the hit-discovery and hit-to-lead steps<br />
into one, with a potentially significant timesaving. The lecture will describe the concept and development of a<br />
disposable “plastic” that is capable of measuring a number of metabolic parameters simultaneously in nanowells<br />
in real-time using optical sensor technology with multiple fluidic inputs/outputs and all associated instrumentation<br />
and software. A key aspect of the approach is the introduction of novel planar optical sensors based on using a<br />
reflection hologram as an inexpensive, disposable, mass-producible indicator of metabolic activity. This approach<br />
is unique in the field of chemical sensors since the hologram per se provides both the analyte-responsive polymer<br />
and the optical interrogation and reporting transducer.<br />
Holographic matrices have been developed which can be modified rationally in order to construct specific<br />
response mechanisms to the target analyte. Defined polymeric matrices such as poly-vinylalcohol, poly-hydroxyeth<br />
ylmethacrylate, poly-acrylamide and starch have been used to fabricate the holograms. Examples of the detection<br />
of a number of analytes, including pH, ions, enzymes and metabolites will be given. These sensors have been<br />
incorporated into a fully instrumented nanophysiometer in order to measure the growth kinetics and metabolic<br />
activity of a number of microbial systems in real-time. This technology has the potential to revolutionize the way<br />
modern microbial, plant and animal cell biology is conducted.
3:00 pm Tuesday, February 3 HT Chemistry Room B3<br />
Erick Carreira<br />
Swiss Federal Institute of Technology – ETH Zürich<br />
ETH Hönggerberg – HCI H207<br />
Zürich, CH-8093 Switzerland<br />
carreira@org.chem.ethz.ch<br />
Natural Products Inspired Studies in Asymmetric Synthesis<br />
Three research programs in target-oriented natural products synthesis will be discussed. Particular emphasis<br />
will be placed on methods discovery and development. Their application to the selected targets as well as their<br />
relevance in addressing broader issues in asymmetric synthesis will be presented. The methods include: the use<br />
of nitrile oxide cycloadditions reaction to furnish a general, modular synthesis of polyketides, and the annulation<br />
reaction of spirocyclopropyl oxindoles and imines as an approach to spiropyrrolidino oxindoles.<br />
3:30 pm Tuesday, February 3 HT Chemistry Room B3<br />
Kevin Burgess<br />
Texas A & M University<br />
P.O. Box 30012<br />
College Station, Texas 77842-3012<br />
burgess@tamu.edu<br />
Solid and Solution-phase Syntheses of Peptidomimetics That Mimic or Disrupt Proteinprotein<br />
Interactions<br />
Solid phase macrocyclization reactions enabled us to identify peptidomimetics that mimic the nerve growth factor<br />
(NGF) and bind to its TrkA receptor giving a functional response. Solution phase method will be described that has<br />
enabled us to construct libraries of fluorescently labeled bivalent molecules, each containing two of these units.<br />
Current efforts are focused on preparation of libraries of much simpler bivalent mimics via similar methodology.<br />
45<br />
PODIUM ABSTRACTS
4:00 pm Tuesday, February 3 HT Chemistry Room B3<br />
Wim Meutermans<br />
Alchemia Pty Ltd<br />
P.O. Box 6242<br />
Upper Mt. Gravatt<br />
Queensland, 4122 Australia<br />
wmeutermans@alchemia.com.au<br />
Tailoring Molecular Diversity Through Carbohydrate-based Scaffolds<br />
VAST drug discovery technology makes use of 3D scaffolds which resemble monosaccharides in structure and<br />
where pharmacophoric substituents are readily attached at up to five positions in a regio- and stereo-controlled<br />
manner. Conformationally rigid products, each with a unique 3D presentation, are produced in parallel using<br />
automated techniques, thereby generating libraries of tailored structural and functional diversity. The nature of<br />
the scaffold and substituents are modeled and designed to selectively target receptors, but also to optimize<br />
ADME properties. Research that resulted in the identification of novel VAST drug leads for selected targets will<br />
be presented, including diversity and modeling considerations, along with ‘in vitro’ and ‘in vivo’ data on activity,<br />
toxicity and pharmacokinetics.<br />
4:30 pm Tuesday, February 3 HT Chemistry Room B3<br />
Jan Marik<br />
University of California, Davis – Cancer Center<br />
4501 X Street<br />
Sacramento, California 95817<br />
jmarik@ucdavis.edu<br />
From “One-Bead One-Compound” to Chemical Microarrays<br />
46<br />
Co-Author(s)<br />
Aimin Song, Ruiwu Liu,<br />
Xiaobing Wang, Alan Lehman,<br />
Kit S. Lam<br />
The era of combinatorial chemistry libraries began with spatially addressable low density arrays like the multipin<br />
system described by Geysen and the spot synthesis of method published by Frank. At the same time, Fodor<br />
et al. reported the light directed synthesis of the first spatially addressable high density chemical microarrays. In<br />
1991, Lam et al. invented the “one-bead one-compound” (OBOC) method for highly efficient synthesis of random<br />
peptide libraries containing millions of peptide beads, such that each bead displays only one peptide entity.<br />
This OBOC library could be also viewed as an ultra high density peptide microarray that is spatially separable<br />
but not addressable. In the last 10 years the OBOC method has been used to prepare encoded small molecule<br />
libraries. Although huge number of compounds can be prepared with the OBOC method, only limited amounts of<br />
compound can be retrieved from one single bead. To increase the capacity of each bead, Schreiber et al. reported<br />
the use of macrobeads that can provide up to 0.5 mmol/bead of compound. In our lab we have recently developed<br />
the one-aggregate one-compound method which has the capacity of generating 1 – 10 mmol of compound<br />
from each aggregate. By applying the “split mix synthesis” approach, a large number of encoded compoundaggregates<br />
can be generated efficiently. The bead aggregates consist of two populations of beads. The majority<br />
of the beads have cleavable linkers and are used for preparation of the testing compound, whereas the minority<br />
population of beads is colored and is used for the preparation of the encoding tag. After synthesis, the testing<br />
compound is released to the solution from the aggregate and can be fed into the standard solution phase high<br />
throughput screen, or selectively ligated to a biopolymer and printed to solid support to form a microarray. Positive<br />
spots or wells can be traced back to the aggregate where the encoding beads can be retrieved for decoding.
8:00 am Wednesday, February 4 HT Chemistry – Analytical Room B3<br />
Götz Schlotterbeck<br />
F. Hoffmann-La Roche Ltd<br />
Grenzacherstrasse 124, Basel<br />
CH-4070 Switzerland<br />
goetz.schlotterbeck@roche.com<br />
Aut<strong>omation</strong> and Miniaturization in NMR<br />
47<br />
Co-Author(s)<br />
Alfred Ross,<br />
Hans Senn<br />
Currently NMR measurements are performed in tubes of 5mm diameter and a detection volume of about<br />
500µL. Miniaturizing the NMR measurement has many advantages the most important are: (i) the intrinsic higher<br />
sensitivity of the miniaturized detection coil leads to a decrease of the experiment time and thus potentially to a<br />
higher sample throughput of mass-limited samples and (ii) the use of costly deuterated solvents can significantly<br />
be reduced, and concomitantly the spectral artifacts from solvent impurities. NMR measurements using a new<br />
1mm TXI Microprobe (Bruker) with an active sample volume of 2.5 µL are reported. This is the first microliter<br />
NMR probe with optimized coil geometry for use with individual capillaries of outer diameter of 1 mm. The probe<br />
offers significant advantages over previous and other analytical-chemical NMR technologies: an increased mass<br />
sensitivity by a factor of 4 compared to the conventional setup and spectra with very low-solvent background. We<br />
demonstrate the potential of the probe for structure elucidation of mass- and volume-limited samples and microbore<br />
HPLC-NMR coupling on selected examples from pharmaceutical research. In addition applications with high<br />
sample throughput (HT-NMR) for quality control of large sample arrays are shown. This also includes the automatic<br />
sample preparation of 1mm capillaries.<br />
8:30 am Wednesday, February 4 HT Chemistry – Analytical Room B3<br />
Russell Scammell<br />
Argenta Discovery Ltd<br />
8/9 Spire Green Centre<br />
Flex Meadow<br />
Harlow, Essex CM19 5TR United Kingdom<br />
russell.scammell@argentadiscovery.com<br />
Aut<strong>omation</strong> and Mass Spectrometry: A Love – Hate Relationship?<br />
Today’s mass spectrometer interface systems operate on principles that have remained fundamentally unchanged<br />
for over 10 years although the method of automated sample introduction has probably experienced the most<br />
significant changes over this time period. This presentation will initially provide a brief history of the interfaces and<br />
sample introduction techniques that have emerged over the past decade. The discussion will move into areas<br />
that are now key to the successful operation of an analytics laboratory supporting both the needs of Discovery<br />
Chemistry and Bioananlysis. The paradigm shift to non MS-centric systems embracing the aut<strong>omation</strong>, integration<br />
and miniaturisation (AIM) concept has greatly impacted on sample introduction. The factors of ever increasing<br />
time pressures and sample nu<strong>mbers</strong> in today’s industry will be shown to have been the single driving force in the<br />
areas of automated sample preparation/purification/analysis, HPLC column technologies, auto-sampler/fraction<br />
collection technologies and interface hardware. The presentation will conclude with a look to the future, including<br />
the area of chip based technologies and how the emergence of multiplexed interfaces are having an impact on<br />
future autosampler designs.<br />
PODIUM ABSTRACTS
9:00 am Wednesday, February 4 HT Chemistry – Analytical Room B3<br />
Dave Rakestraw<br />
Eksigent Technologies<br />
2021 Las Positas Court, Suite 161<br />
Livermore, California 94550<br />
djrakestraw@eksigent.com<br />
Rapid, High Resolution Multiplexed HPLC System<br />
48<br />
Co-Author(s)<br />
Doug Cyr, Roger Farrow,<br />
Karen Hahnenberger, Don Arnold,<br />
David Neyer, Jason Rehm,<br />
Ken Hencken, Philip Paul<br />
The use of HPLC for high throughput analysis has been limited by the time required for chromatographic<br />
separations and the number of individual instruments that can be dedicated to the application. We will present<br />
data from a new high performance liquid chromatography platform capable of performing ~10,000 separations<br />
per day. This high throughput has been made possible by extremely rapid gradient delivery that dramatically<br />
reduces traditional separation times and the development of a single instrument capable of performing 8 parallel<br />
separations. Low delay volumes enable the use of rapid gradients with durations as low as 10 seconds. The<br />
rapid gradients are achieved using Eksigent’s Microfluidic Flow Control (MFC) system, and result in retention time<br />
repeatability of
10:30 am Wednesday, February 4 HT Chemistry – Microscale Room B3<br />
Miryam Fernandez Suarez<br />
GlaxoSmithKline Pharmaceuticals<br />
GSK, CTC, University Chemical Labs<br />
Lensfield Road<br />
Cambridge, CB2 1EW United Kingdom<br />
miryam_2_fernandez-suarez@gsk.com<br />
Microfluidic Platforms for Drug Discovery<br />
49<br />
Co-Author(s)<br />
Stephanie Y. F. Wong<br />
Brian H. Warrington<br />
The competitive world of drug market, under cost reduction pressures and imminent expiration of patents, forces<br />
pharmaceutical companies to an expensive search for suitable novel leads. Combinatorial chemistry and high<br />
throughput screening have proved to be useful for discovering potential drug candidates. However, these costly<br />
techniques did not satisfy the high expectations in terms of number of drug candidates entering development<br />
stages, as they hardly scratch the enormous and diverse universe of the possible compounds and even can lead<br />
to logistic problems of reagent consumption, sample storage and waste production. In response to the competing<br />
pressures for increasing output and reducing time scales and costs, the pharmaceutical industry is in the midst of<br />
a technology-driven revolution. In this contest, microfluidic based technologies show a great potential owing to its<br />
high degree of integration with modern miniaturised analysis and screening techniques. It also offers advantages<br />
in terms of shorter response times, reduction of reagent consumption and waste volume. Our aim is to perform<br />
multiple functions such as synthesis, detection, separation, and screening all integrated in a microfabricated<br />
device, exploiting the advantages of combining microfluidics and electronic components and provided with<br />
software tools for optimization. In this presentation, we will review the approach of GSK and partners to exploit<br />
the advantages of microfluidics in the development of miniaturized chemistry platforms, from our initial well-based<br />
approaches, to our continuous flow automated micro reactor systems. In addition, we will present our latest<br />
developments towards multi-step synthesis protocols and coupling with screening assays.<br />
11:00 am Wednesday, February 4 HT Chemistry – Microscale Room B3<br />
Mike Pollard<br />
Syrris<br />
27 Jarman Way<br />
Royston, Herts, SG8 5HW United Kingdom<br />
mike.pollard@syrris.com<br />
Real-world Microchemistry: Milligram Scale Organic Synthesis in Microreactors<br />
Microreactors are an emerging technology, offering great promise for productivity gains in drug discovery and<br />
development. Some early presentations made promises of “magic” gains in yield and reaction speed, claimed as<br />
achievable as a direct result of the channel geometry and fluidic design of these devices. However, these gains<br />
have not been consistently demonstrated. Medicinal chemists have rightly remained sceptical that a chip-based<br />
reactor system can offer ease of use coupled with delivering a realistic quantity of the desired compound. Syrris<br />
has now been working for two years to develop the technologies needed to make Microreactors a real world<br />
proposition. From the start of its development program, Syrris has focused on the medicinal chemist’s need for<br />
a robust, practical, low cost tool, capable of reliably synthesising milligram quantities of compound. We have<br />
recently carried out an extensive test program to validate the performance of Syrris Microreactor chips over a wide<br />
range of organic synthesis tasks. The tests addressed issues such as: the range of synthesis protocols that could<br />
be undertaken, reaction rate, yield, and automatically handling real world problems such as precipitation. This<br />
paper presents details of the synthesis work carried out, including a realistic assessment of the best application<br />
area for Microreactors. We will present a preview of the forthcoming Syrris AutoR medicinal chemistry tool,<br />
being developed as part of our strategy to understand and meet the needs of the R&D scientist though research,<br />
collaboration, and product development.<br />
PODIUM ABSTRACTS
11:30 am Wednesday, February 4 HT Chemistry – Microscale Room B3<br />
Klavs Jensen<br />
Massachussetts Institute of Technology<br />
77 Massachusetts Avenue, MIT 66-566<br />
Cambridge, Massachusetts 02139<br />
kfjensen@mit.edu<br />
Microscale Synthesis – Integration of Reactions and Separations<br />
The microscale revolution in chemistry and biology promises to transform classical batch wise laboratory<br />
procedures into integrated systems capable of proving new understanding of fundamental chemical and biological<br />
processes as well as rapid, continuous discovery and development of new products with less use of resources<br />
and waste generation. Potential applications and advantages of microchemical systems are demonstrated with<br />
cases studies drawn from liquid phase organic chemistry, including glycosylation and reactions usually performed<br />
at cryogenic conditions. Other reaction examples are gas-liquid reactions, such as direct fluorination and<br />
ozonolysis, as well as synthesis and modification of colloidal nanoparticles. Microscale separation of gas-liquid,<br />
immiscible liquids, and solid liquid mixtures are demonstrated with examples relevant to solvent extraction, solvent<br />
switch, gas removal, and nucleation. Finally, micro reaction, separation, and analytical schemes are combined<br />
into integrated microsystems for multistep synthesis with applications for discovery as well as development.<br />
Optimization of reaction conditions serves to exemplify the latter application. We discuss challenges remaining to<br />
the routine implementation of microscale synthesis in automated laboratory procedures.<br />
12:00 pm Wednesday, February 4 HT Chemistry – Microscale Room B3<br />
Thomas Schwalbe<br />
CPC – Cellular Process Chemistry Systems GmbH<br />
Hanauer Landstrasse 526/G58<br />
Frankfurt, 60343 Germany<br />
schwalbe@cpc-net.com<br />
CYTOS Continuous Chemistry – A Coherent Chemical Synthesis Technology to Lever Drug<br />
Innovation Process Value Generation<br />
The drug innovation process employed by companies today suffers from economical inadequacies. Emphasis<br />
has lately been attributed to balancing the flow of projects through the various stages required in the progression<br />
of projects to the market. Chemistry is a key area of concern, from discovery and up to proof of concept, for<br />
a new drug application. Several chemical disciplines require scarce specialized human resources while project<br />
progression requires increasing capital expenditure, particularly when a process is transferred to the pilot plant.<br />
Target structures increasingly aim at novel molecular topography and often require high energy synthetic methods<br />
to construct constrained moieties. Scaling such high energy synthesis routes presents selectivity challenges,<br />
particularly when these are addressed by thermal control. In many cases, constraints at the process level means<br />
that scaling requires time-consuming development of new synthetic procedures, which can be a significant<br />
bottleneck to producing complex targets in sufficient quantities for proof of concept studies. An alternative<br />
approach is to carry out synthesis by continuous flow reactions using processing systems of minimal internal<br />
volume. By avoiding the issues of increasing batch sizes, continuous flow chemistry enables direct scaling to be<br />
carried out using the exact same process. The Cytos ® system has been designed to perform continuous flow<br />
reactions in research, and can be used for the production of a compound in any quantity by the research synthetic<br />
process. The specific design of Cytos ® reactors ensures both improved mixing and heat exchange as compared to<br />
batch equipment, hence allowing the trouble-free conduct of otherwise hard to scale chemistries.<br />
50
3:30 pm Wednesday, February 4 HT Chemistry – New Strategies Room B3<br />
Mark Bradley<br />
University of Southampton<br />
Highfield, Southampton<br />
Hants, SO17 1BJ United Kingdom<br />
mb14@soton.ac.uk<br />
Real Time Combinatorial Arrays and Assays for Proteases, Kinases, and Transfection Agents<br />
This presentation will cover the interactions between biology, chemistry and chemical technology and will<br />
include: (a). New, highly sensitive method for protease (or kinase) analysis and screening, with the potential to<br />
screen 100,000 substrates in a single pass. (b). Chip based library screening and cellular binding assays. (c). The<br />
development of highly efficient molecular transporters for both single cells and slice cultures and array based<br />
screening of libraries of molecular transporters to enable the development of “transfection chips”.<br />
4:00 pm Wednesday, February 4 HT Chemistry – New Strategies Room B3<br />
Thomas Smith<br />
GlaxoSmithKline<br />
New Frontiers Science Park (North)<br />
Third Avenue<br />
Harlow, Essex, CM19 5AW United Kingdom<br />
thomas_j_smith@gsk.com<br />
High Throughput Drug Discovery<br />
The pharmaceutical industry is coming under increasing pressure to deliver new medicines and speed up the drug<br />
discovery process. Hence, chemists are looking for ways to improve their methods of compound production. The<br />
traditional one product at a time approaches are now giving way to faster and more cost effective, high throughput<br />
chemistry methodologies. Routinely, robotic chemical synthesisers are used to carry out parallel reactions on<br />
multiple substrates. Hundreds to thousands of compounds can now be prepared, analyzed and purified in<br />
the time taken to prepare just a few by conventional methods. The presentation will focus on the aut<strong>omation</strong><br />
and technology used at GSK to achieve high throughput compound production and purification as well as the<br />
importance of integrating the many components of this complex process.<br />
51<br />
PODIUM ABSTRACTS
4:30 pm Wednesday, February 4 HT Chemistry – New Strategies Room B3<br />
Jonathan Ellman<br />
University of California, Berkeley<br />
826 Latimer Hall<br />
Berkeley, California 94720-1460<br />
jellman@uclink.berkeley.edu<br />
New Approaches for High Throughput Heterocycle Synthesis<br />
The first approach is based upon enantiomerically pure tert-butanesulfinamide, which has been developed in my<br />
laboratories. This chiral amine reagent is now employed extensively in the pharmaceutical industry. Methods will<br />
be presented for the parallel synthesis of a wide range of pharmaceutically relevant compounds from chiral amine<br />
building blocks to complex alkaloids using either solid-phase or solution-phase methods. In the second approach,<br />
C-H functionalization of organic compounds has been applied to the preparation of pharmaceutically relevant<br />
structures, including substituted indanes, tetralanes, indoles, dihydrobenzofurans, dihydroindoles, and azoles. The<br />
application of microwave chemistry to both of these approaches will also be described.<br />
5:00 pm Wednesday, February 4 HT Chemistry – New Strategies Room B3<br />
Frantisek Turecek<br />
University of Washington<br />
Bagley Hall<br />
Box 351700<br />
Seattle, Washington 98195-1700<br />
turecek@chem.washington.edu<br />
52<br />
Co-Author(s)<br />
Yuko Ogata<br />
Erkang Fan<br />
Aut<strong>omation</strong> of Bioanalytical Processes Using the Lab-on-Valve Approach. Applications to<br />
Protein Binding and Diagnosis of Inborn Errors of Metabolism<br />
A method is introduced that achieves quantitative screening of ligands for binding with immobilized proteins.<br />
The method uses the Lab-on-Valve apparatus that is interfaced to an electrospray ionization mass spectrometer<br />
(ESI-MS), such as a quadrupole ion trap or a tandem triple quadrupole instrument. Proteins are immobilized on<br />
a solid support by covalent attachment to CNBr activated sepharose, or by non-covalent binding via biotin or<br />
His-tag conjugates to streptavidin-agarose or IMAC beads. The support with the immobilized protein is infused in<br />
the Lab-on-Valve apparatus and exposed to a mixture of potentially binding ligands, which are eluted in a zone<br />
frontal mode. Mass spectrometric analysis is used to monitor the concentrations of the eluted components and<br />
measure their breakthrough volumes. The latter are used for the calculation of binding constants for the mixture<br />
components. Examples will be given for ligand binding to cholera toxin B subunit and human peroxin PEX5.
8:00 am Thursday, February 5 HT Chemistry – Informatics Room B3<br />
Bernard Choi<br />
Merck Research Laboratories<br />
P.O. Box 2000<br />
Rahway, New Jersey 07065<br />
bernard_choi@merck.com<br />
53<br />
Co-Author(s)<br />
Athanasios Tsipouras<br />
High Throughput Quality Control LC-MS Analysis: Data Acquisition and Interpretation<br />
A high throughput analytical characterization system was developed for quality control support of a central<br />
sample collection resource. This system utilizes liquid chromatography mass spectrometry, UV absorption, and<br />
evaporative light scattering detection. Continuous operation of analytical instrumentation is accomplished by fully<br />
automating sample submission and report processing functions with in-house developed software. Comprehensive<br />
analytical information characteristic of quality, chemical, and physical properties (e.g., relative purity, detection<br />
sensitivity, retention properties) are extracted from the report data and transferred to an online Oracle database.<br />
Sample quality is assessed by utilizing multiple result criteria: intensity, signal-to noise, percent base peak intensity<br />
and structural similarity. Application of the comprehensive analytical data for discovery applications will be also<br />
discussed.<br />
8:30 am Thursday, February 5 HT Chemistry – Informatics Room B3<br />
Tudor Oprea<br />
University of New Mexico<br />
School of Medicine<br />
MSC 08 4560<br />
Albuquerque, New Mexico 87131-0001<br />
toprea@salud.unm.edu<br />
Navigating Large Chemical Spaces<br />
Co-Author(s)<br />
Marius Olah, John Tallarico,<br />
Erik Brauner, Tharun Kumar,<br />
Cristian Bologa<br />
As (virtual) chemical space becomes infinite, methods to navigate in chemical property and chemical structure<br />
space are gaining relevance. Two methods, ChemGPS (chemical global positioning system) and SimNav (similarity<br />
navigator) will be introduced as means to investigate the chemistry space that is relevant to drug discovery.<br />
ChemGPS compares molecules in property spaces, whereas SimNav uses the similarity principle to prioritize<br />
compounds for biological screening.<br />
PODIUM ABSTRACTS
9:00 am Thursday, February 5 HT Chemistry – Informatics Room B3<br />
Ping Du<br />
Du Consulting, LLC<br />
4 Fullerton Place<br />
Livingston, New Jersey 07039<br />
ping_du_2003@yahoo.com<br />
An Informatics System for Peptide Drug Discovery<br />
An integrated informatics system has been developed at Adaptive Therapeutics to support the discovery of cyclic<br />
peptides as antibacterial agents. The workflow supported includes combinatorial library synthesis, sequencing,<br />
screening, cherry-picking for hit confirmation, scale up synthesis, and dose response microbiology assays. Built on<br />
Oracle and Microsoft.Net technologies, the system consists of three tiers, database, business logic components,<br />
and client application modules. It is integrated with multiple laboratory instruments, such as Tecan liquid handler,<br />
Hitachi LCMS, plate readers, and barcode label printers.<br />
9:30 am Thursday, February 5 HT Chemistry – Informatics Room B3<br />
Neil Benn<br />
Cambridge Antibody Technology Limited<br />
Milstein Building<br />
Granta Park<br />
Cambridge, Cambs SG1 6GH United Kingdom<br />
neil.benn@cambridgeantibody.com<br />
Process Definition and Evaluation Utilizing Automated Metrics Gathering<br />
The Laboratory Aut<strong>omation</strong> industry is starting to come of age. Equipment released by manufacturers is of ever<br />
increasing quality, both in functionality and reliability. In addition users of aut<strong>omation</strong> are seeing real and tangible<br />
benefits from the implementation of aut<strong>omation</strong> within their laboratory. However the wide range of equipment<br />
and varying standards in both hardware and software often means that it is difficult for the laboratory manager<br />
to choose which equipment to purchase and also to measure how well that equipment is working within the<br />
established process. It is difficult to answer questions such as: Is the equipment being used? How can I modify<br />
the process to maximize return on the aut<strong>omation</strong> investment? Traditionaly this would have been acheived by<br />
talking to people who work the process in question. However this is time-consuming both for the user and the<br />
team collating the information and such ‘soft’ data can also be difficult to interpret and analyse. This presentation<br />
will describe the implementation of a system to automatically gather ‘hard’ metrics data of equipment usage that<br />
will not be biased by user opinions. The system is designed to be flexible enough to work with existing control<br />
software and not require major effort to incorporate in new equipment. Finally methods of analysis and conclusions<br />
from analysis will be presented.<br />
54
10:30 am Thursday, February 5 High Throughput Screening — ADME – Tox Room A2<br />
Arun Mandagere<br />
Pfizer Global Research and Development<br />
006/460, 2800 Plymouth Road<br />
Ann Arbor, Michigan 48105<br />
arun.mandagere@pfizer.com<br />
Role of Automated High Throughout Nephelometric Aqueous Solubility System in<br />
Early Drug Discovery<br />
We describe an automated high throughput Nephelometric aqueous solubility system that has played a critical<br />
role in the successful selection of quality lead candidates in early Drug Discovery programs. Aqueous solubility is<br />
used to gauge dissolution, adsorption and bioavailability of lead compounds. The advantage of a Nephelometric<br />
solubility screen is its ability to measure solubility at a comparable rate to secondary HTS pharmacological screens<br />
with minimal sample requirement. This process enables in the rapid identification of poorly soluble compounds<br />
with good activity, which are very likely to fail due to poor adsorption or low bioavailability. More importantly, this<br />
process provides timely feedback to medicinal chemist to pursue synthetic strategies in the producing compounds<br />
with good activity and solubility. Unlike the classical thermodynamic solubility measurements, the Nephelometric<br />
HTS solubility system is uniquely suited for early drug discovery because its speed, high capacity and small<br />
sample requirement. We will highlight the system components, assay parameters, and automated data analysis<br />
software used in building this automated solubility screening system. The Nephelometric HTS Solubility System<br />
increased sample throughput from 100 to 1800 compounds per week, and a 75% reduction in compound usage<br />
when compared to the HPLC- based flow-injection analysis (FIA) solubility method. The results generated by the<br />
HTS Nephelometric solubility method show a good agreement with those of HPLC-FIA solubility method.<br />
11:00 am Thursday, February 5 High Throughput Screening — ADME – Tox Room A2<br />
Ken Matuszak<br />
Abbott Laboratories<br />
100 Abbott Park Road<br />
Abbott Park, Illinois 60064-6122<br />
ken.matuszak@abbott.com<br />
Higher Throughput Strategies for Support of Early ADME In-vitro Screening<br />
What is “high throughput”? In many ways, for an analytical chemist, this is equivalent to the question of what is<br />
“zero.” For some applications (such as the structural elucidation of a completely unknown chemical entity), high<br />
throughput could mean just one complete analysis per day. For others (such as an assay for a specific drug in<br />
plasma samples from clinical studies) it might mean thousands of samples analyzed, quantitated, and reported<br />
in a 24-hour period. This presentation will focus on practical techniques for increasing throughput (“higher<br />
throughput”) for the analytical support of in vitro ADME screens (plasma protein binding, metabolic stability, and<br />
permeability) that are used in the support of Discovery research. While a few corporations have fully automated<br />
this type of work, and have already optimized their sample analysis throughput, many companies continue to use<br />
more traditional tried and true methods. These methods, while robust and reproducible, are far from optimized<br />
for maximum throughput. Methods discussed will include high throughput universal gradients coupled with mass<br />
spectrometric (MS) analysis, parallel solid phase extraction (SPE) and sequential elution to an MS, and quadrupoletime<br />
of flight MS vs. triple quadrupole MS.<br />
55<br />
PODIUM ABSTRACTS
11:30 am Thursday, February 5 High Throughput Screening — ADME – Tox Room A2<br />
Alexandra Heinloth<br />
NIEHS, 111 Alexander Drive<br />
Research Triangle Park, North Carolina 27709<br />
heinloth@niehs.nih.gov<br />
The Role of Genomics in Toxicology<br />
56<br />
Co-Author(s)<br />
Richard D. Irwin, Gary A. Boorman<br />
Paul Nettesheim, Leping Li, Raymond W. Tennant<br />
Michael L. Cunningham, Richard S. Paules<br />
Traditional toxicology deals with measuring endpoints of toxicity, evaluating one compound at a time in a very<br />
time consuming manner. The classic parameters of toxicity evaluated in such efforts are dose and time dependent<br />
and rarely allow for predictions of toxicity. One of the main challenges for toxicologists in the 21st century is the<br />
identification of highly sensitive and accurate predictive biomarkers for exposure, pharmacological effect and<br />
toxicity of environmental hazards. An extremely promising tool to aid in this effort is the genome-wide analysis<br />
of gene expression, from which scientists may extrapolate predictive signatures of exposure and effects. This<br />
would allow scientists to rapidly classify unknown compounds as being similar to groups of known compounds,<br />
thus potentially predicting their beneficial and adverse effects. In order to accomplish this goal we are creating<br />
a knowledge base of gene expression patterns. One model substance examined within this enterprise is<br />
acetaminophen as a representative compound inducing acute hepatotoxicity. We exposed rats to a wide doserange<br />
of acetaminophen (from non-toxic to severely toxic) and examined gene expression responses. We were<br />
able to extrapolate gene signatures identified following exposures to non-toxic doses which indicated the potential<br />
toxicity of this substance and which were observed to increase in magnitude in progression with increasingly<br />
toxic doses. The goal is to develop signatures that can serve as predictive biomarkers in the screening process of<br />
unknown compounds for hepatotoxicity.<br />
1:30 pm Thursday, February 5 Drug Discovery Case Studies Room B1<br />
Alexander Alanine<br />
F. Hoffmann-La Roche Ltd<br />
Grenzacherstrasse 124<br />
Basel, CH-4070 Switzerland<br />
alexander.alanine@roche.com<br />
Hit and Lead Generation Beyond High Throughput Screening<br />
The identification of small molecule modulators of protein function and the process of transforming these into<br />
high content lead series are critical activities in modern drug discovery. The key decisions made during this<br />
process have far reaching effects down-stream for success in lead optimization and even more critically in clinical<br />
development. Recent focus on these activities has been driven by the increasing costs resulting from the high<br />
clinical failure rates as well as untapped opportunities emerging from the efforts in functional genomics.
2:00 pm Thursday, February 5 Drug Discovery Case Studies Room B1<br />
Esteban Pombo-Villar<br />
Novartis Pharma Ltd.<br />
WSJ-386.07.15<br />
Basel, CH-4002 Switzerland<br />
esteban.pombo@pharma.novartis.com<br />
Alliances in Technology<br />
Developments in technology are often the result of formal or informal alliances. Formal alliances to develop a<br />
specific technology, such as collaborations, licensing agreements, joint ventures and consortia, can be successful<br />
in providing a useful product and some competitive advantage to the parties. These alliances are common in the<br />
pharmaceutical industry, for example, where the exclusivity of the product is one of the main drivers of competitive<br />
advantage. Another type of process however can be successful in delivering a generally useful technology – this<br />
is the case of informal partnerships between potential users and developers. The model used by the software<br />
industry, of having a user group to test the beta version of an eventual commercial package. In this case, the<br />
needs of a specific market are incorporated into the product design, and together with the commercial motivation<br />
of the developer, the final product may be more practical, and in the end deliver more value to the customers.<br />
2:30 pm Thursday, February 5 Drug Discovery Case Studies Room B1<br />
Fred Pritchard<br />
MDS Pharma Services<br />
3504 Proprietor Way<br />
Raleigh, North Carolina 27612<br />
fred.pritchard@mdsps.com<br />
Understanding Risk and Value: Decision Gates in Drug Development<br />
Aut<strong>omation</strong> has changed the process of drug discovery from managed serendipidy to engineered selection.<br />
Nevertheless, the promise of more drugs with high specificity and selectivity, low toxicity and optimal delivery<br />
achieving clinical success has not yet met the expections of the pharmaceutical, medical and investment<br />
communities. Predicting how a drug will behave in humans prior to clinical testing requires a battery of<br />
sophisticated in vitro tests that complement traditional in vivo animal safety assessments. This presentation will<br />
describe how to strategically identify which non-clinical studies should be performed to provide the required<br />
guidance and comfort to stakeholders involved in clinical drug testing. In addition, key factors that can influence<br />
risk and value in the decision process will be addressed.<br />
57<br />
PODIUM ABSTRACTS
3:00 pm Thursday, February 5 Discovery Case Studies Room B1<br />
Walter Sneader<br />
University of Strathclyde – Institute for Biomedical Sciences<br />
27 Taylor Street<br />
Glasgow, Scotland, G4 0NR United Kingdom<br />
w.e.sneader@strath.ac.uk<br />
Drug Prototypes – What We Learn From History<br />
Pharmaceutical historian Walter Sneader looks at the limited variety of ways in which novel drugs were discovered<br />
during the 19th and 20th centuries and how these have been largely exhausted. The consequence is that it has<br />
now become exceedingly difficult to develop new drugs that are innovative. Current approaches to confronting this<br />
issue are discussed.<br />
3:00 pm Tuesday, February 3 High Throughput Screening – Ion Channels Room A2<br />
Jennings Worley<br />
Amphora Discovery Corporation<br />
800-4 Capitola Drive<br />
Research Triangle Park, North Carolina 27709<br />
jennings.worley@amphoracorp.com<br />
Higher Throughput Electrophysiology and Ion Channel Assays Technologies; Matching<br />
Targets, Throughput and Target Prosecution Objectives<br />
Despite the recognized importance of ion channels as therapeutic targets, significant challenges exist in<br />
developing facile and robust methods for detecting cellular ion transport to support rapid functional analysis<br />
in drug discovery. Technologies were first developed that transitioned single cell fluorescent measurements of<br />
permeate ions or membrane potential that can result from ion channel function to plate-based formats. Application<br />
of these and other ion detection technologies underpin the industry’s ability to apply HTS formats to measures<br />
of ion channel function. This increase in capacity of library screening has now placed significant pressures<br />
on traditional electrophysiology which, despite the power of these direct measurements, is mired by very low<br />
throughput. Over the last few years, various technologies have been introduced which represent an evolution<br />
of traditional electrophysiology by addressing throughput restrictions. For the most part, these technology<br />
platforms vary from automated electrophysiology to plate-based formats and are preparing to significantly<br />
impact chemistry progression by increasing the capacity of voltage clamp measurements of cells up to >50<br />
fold. The overall maturation of ion channel screening technologies and methods has, for the first time, provided<br />
a broadening collection of assay tools. Because of the complexity and breath of the ion channel target class,<br />
matching technology to specific channel types as well the appropriate phase of drug discovery has now become<br />
an important determinant of project planning. The presentation will endeavor to address strategic use of these<br />
technologies, highlighting their strengths and weaknesses, as they are poised to redefine project progression<br />
pathways and objectives.<br />
58
3:30 pm Tuesday, February 3 High Throughput Screening – Ion Channels Room A2<br />
Chris Mathes<br />
Axon Instruments, Inc.<br />
3280 Whipple Road<br />
Union City, California 94587<br />
chrism@axon.com<br />
PatchXpress: Automated Patch Clamp for High-quality, High Throughput Recordings of Both<br />
Voltage and Ligand-gated Ion Channels<br />
Because they play important roles in cell physiology, such as excitability and gene expression, ion channels<br />
are ideal drug discovery targets. Ion channels have been a relatively untapped resource in the world of drug<br />
discovery. No means have existed to directly measure ion channel activity in a high throughput patch-clamp assay,<br />
so researchers have used other, less direct techniques for screening. The PatchXpress 7000A changes all this,<br />
opening up a huge pool of highly “drugable” ion channel targets. The PatchXpress records currents through both<br />
voltage and ligand-gated channels. An important example of a voltage-gated channel is the human ether-a-gogorelated<br />
gene (hERG) channel. Accurate screening against hERG channels is an essential step in the drug discovery<br />
process. In general, lead compounds are tested against hERG channels for safety reasons. Safety pharmacology<br />
requires high quality and dependable data from hERG screens for decisions regarding the fate of lead compounds.<br />
The PatchXpress is an efficient tool for automatically recording reliable hERG currents from 16 cells simultaneously.<br />
The PatchXpress uses planar patch-clamp electrodes, called SealChips, made exclusively for Axon by Aviva<br />
Biosciences. These electrodes provide high resistance giga-ohm seals for high-quality patch-clamp recordings.<br />
With the PatchXpress, sophisticated protocols can be performed, complete with full dose-response experiments<br />
on individual cells. Alternatively, the PatchXpress can be used for rapidly screening hundreds of compounds per<br />
day. When it comes to safety testing, high-quality recordings are essential and the PatchXpress provides the speed<br />
and confidence required for effective hERG screening. Data from PatchXpress users will be presented.<br />
4:00 pm Tuesday, February 3 High Throughput Screening – Ion Channels Room A2<br />
Jia Xu<br />
AVIVA Biosciences Corporation<br />
11180 Roselle Street, Suite 200<br />
San Diego, California 92121<br />
jxu@avivabio.com<br />
Improving Recording Quality for High Throughput Patch Clamp<br />
Recording quality is of central concern for high throughput patch clamp screening. It is determined by the both the<br />
quality of the biochip and the quality of the cell preparation. Our new generation of SealChip has stable Rm and<br />
low Ra performance while maintaining high gigaseal rate. Our cell preparation methods are designed to optimize<br />
seal rate, Rm, Ra and stability performance. AVIVA is improving its SealChip products continuously to meet our<br />
customers performance requirements.<br />
59<br />
PODIUM ABSTRACTS
4:30 pm Tuesday, February 3 High Throughput Screening – Ion Channels Room A2<br />
Christa Nutzhorn<br />
CYTOCENTRICS GmbH<br />
Taeleswiesenstraße 3<br />
Reutlingen, 72770 Germany<br />
vanbergen@cytocentrics.com<br />
CytoPatch – Automated Patch Clamping<br />
60<br />
Co-Author(s)<br />
Thomas Knott<br />
Alfred Stett<br />
Timm Danker<br />
The gold standard of ion channel analysis is patch clamping. This manual technique is time and cost consuming.<br />
Even a good electrophysiologist might patch only ten cells per day. This is a bottleneck for the pharmaceutical<br />
industry who wants to screen thousands of compounds. Aut<strong>omation</strong> of patch clamping is a reliable approach<br />
for ion channel drug discovery. CYTOCENTRICS has methods ready to run for voltage-gated and ligand-gated<br />
ion channels. Its modular CytoPatch accelerates discovery, characterisation and screening of novel drug<br />
compounds. This technology is top edge in quality, flexibility and high throughput. It reduces time and costs<br />
significantly.<br />
8:00 am Wednesday, February 4 High Throughput Screening – Informatics Room A2<br />
Roy Goodacre<br />
UMIST<br />
P.O. Box 88<br />
Sackville Street<br />
Manchester, M60 1QD United Kingdom<br />
R.Goodacre@umist.ac.uk<br />
Interpretation of Metabolomic Data Using Explanatory Machine Learning<br />
Post-genomic science is producing bounteous data floods, and the extraction of the most meaningful parts of<br />
these data is key to the generation of useful new knowledge. A typical metabolic fingerprint or metabolomics<br />
experiment is expected to generate thousands of data points (samples times variables) of which only a handful<br />
might be needed to describe the problem adequately. Rule induction (RI) methods and evolutionary algorithms<br />
(EAs) are ideal strategies for mining such data to generate useful relationships, rules and predictions. This<br />
presentation will give an overview of some of the metabolomic studies that are currently in progress in UMIST<br />
that exploit these explanatory machine learning algorithms. Within this context we have been developing Fourier<br />
transform infrared (FT-IR) spectrospcopy as a high throughput (1 s is typical per sample) “holistic” metabolic<br />
fingerprinting screening approach and flow-injection electrospray ionization mass spectrometry (FI-ESI-MS) as a<br />
metabolic profiling technique. The following examples will be presented: (1) the detection of the adulteration of<br />
virgin olive oil, (2) the detection of a spore-specific chemical biomarker in bacterial spores; and (3) the quantitative<br />
detection of metabolic markers for food spoilage.
8:30 am Wednesday, February 4 High Throughput Screening – Informatics Room A2<br />
Nick Haan<br />
BlueGnome Ltd<br />
St John, Cambridge, CB4 0WS United Kingdom<br />
nick.haan@cambridgebluegnome.com<br />
Bayesian Approaches to the Analysis of High Throughput Experimental Data:<br />
Using What We Know to Discover What We Don’t<br />
Statistical approaches are routinely used to interpret the results of high throughput experimental processes.<br />
They have not however been widely applied to the problem of extracting those results from the raw assay data<br />
where process related variability presents severe challenges to traditional threshold based techniques. Bayesian<br />
approaches provide a rigorous means by which all grades of prior knowledge relating to the assay may be used<br />
in conjunction with the data itself in order to improve hit detection. An additional benefit of this approach is that<br />
the resulting statistical framework generates robust confidence estimates in all results based on direct observation<br />
of the experimental process. Confidence estimates may be used to pin-point those areas where process<br />
improvements will translate directly into improved results. In the longer term a confidence framework promises<br />
to provide a rigorous means of combining results from multiple experiments and experimental processes. This<br />
presentation will contrast traditional threshold based techniques with emerging statistical approaches. Examples<br />
will draw heavily on experimental data rather than mathematical formulae to ensure this interesting field of statistics<br />
remains accessible to the widest audience.<br />
9:00 am Wednesday, February 4 High Throughput Screening – Informatics Room A2<br />
Ramesh Padmanabha<br />
Bristol-Myers Squibb Co.<br />
F436A, #5 Research Parkway<br />
Wallingford, Connecticut 06492<br />
Ramesh.Padmanabha@bms.com<br />
Getting the Most From Your HTS: Quality Control and Data Mining<br />
61<br />
Co-Author(s)<br />
James Gill<br />
False negatives and false positives are unavoidable issues in any HTS campaign. Various strategies have been<br />
devised to minimize their impact and to increase the quality of hits identified through HTS. Stringent quality control<br />
measures through all aspects of the screening process and appropriate secondary screens help minimize the false<br />
positive rate, while improving the quality of primary screening data. Increasing confidence in the quality of primary<br />
screening data allow for real-time data mining to help identify false negatives. The talk will discuss the approaches<br />
taken to address these aspects of HTS. These include process enhancements, on-line QC and data analysis. A<br />
discussion on using Bayesian categorization models to help identify false-negatives will also be presented.<br />
PODIUM ABSTRACTS
9:30 am Wednesday, February 4 High Throughput Screening – Informatics Room A2<br />
Martin Daffertshofer<br />
Evotec Technologies GmbH<br />
Schnackenburgallee 114<br />
Hamburg, 22525 Germany<br />
martin.daffertshofer@evotec.technologies.com<br />
Information Technology for High Content Screening of Miniaturized Cellular Assays<br />
62<br />
Co-Author(s)<br />
Paavo Helde<br />
Olavi Ollikainen<br />
The need for miniaturised cellular assays has grown in the pharmaceutical industry and so does the need for<br />
high throughput and high content screening platforms of living cells. In this talk we will present experiences in<br />
cellular µHTS using the EVOscreen Mark III platform including the high content confocal imaging system Opera. IT<br />
challenges for the analysis and data management of 200000 cell images in 2h hours will be discussed in detail.<br />
10:30 am Wednesday, February 4 High Throughput Screening – Analytical Room A2<br />
Jörg Stappert<br />
Greiner Bio-One<br />
Maybachstrasse 2<br />
Frickenhausen, 72636 Germany<br />
Joerg.stappert@gbo.com<br />
HTAPlate: Unique 96-Well Plate for DNA- and Protein Arrays<br />
Co-Author(s)<br />
Heinrich Jehle, Günther Knebel<br />
Greiner Bio-One<br />
Florian Winner<br />
Lambda GmbH<br />
DNA- and protein microarrays are a proven tool in academic research, but several obstacles have to overcome<br />
before entering the diagnostic market. Glass slides, the ‘classical’ platform for microarrays, are an inappropriate<br />
platform as sample nu<strong>mbers</strong> are very restricted, the dimensions are not robot friendly and the robustness for<br />
clinical laboratories is missing. In order to overcome these major drawbacks, GBO has developed the first versatile<br />
96-well microplate, designated HTAPlate (High Throughput Array), which has been exclusively designed for DNA-<br />
or protein arrays. This HTAPlate overcomes all restrictions associated with standard 96-well microplates, as special<br />
features have been incorporated, e.g., low rim wells for spotting on the fly, easy access to the wells for top and<br />
bottom reading, volume adjustment for washing and hybridization. We present data proving the robustness of this<br />
new platform using our commercial available Periodontitis test (ParoCheck).
11:00 am Wednesday, February 4 High Throughput Screening – Analytical Room A2<br />
Paren Patel<br />
Nanostream<br />
580 Sierra Madre Villa<br />
Pasadena, California 91107<br />
paren.patel@nanostream.com<br />
Accelerating Discovery Analytical Chemistry Through Micro Parallel Liquid Chromatography<br />
The Nanostream Veloce system – which includes an instrument, software, and replaceable microfluidic cartridges –<br />
incorporates pressure-driven flow to achieve chromatograms comparable to conventional HPLC instrumentation<br />
while offering a dramatic increase in sample analysis capacity. The system enables parallel chromatographic<br />
separations and simultaneous, real-time UV detection for rapid access to analytical results. Each Nanostream<br />
Brio cartridge, made of polymeric materials, incorporates twenty-four columns packed with various stationary<br />
phase materials to achieve reverse phase separations. Mixing and distribution of the mobile phase to each of the<br />
24 columns is precisely controlled in each cartridge. This presentation demonstrates specific benefits of the Veloce<br />
system for a range of applications, from compound library management to protein digests to physicochemical<br />
property testing in ADMET. Future directions will also be discussed.<br />
11:30 am Wednesday, February 4 High Throughput Screening – Analytical Room A2<br />
Richard Ellson<br />
Picoliter, Inc.<br />
1190 Borregas<br />
Sunnyvale, California 94089<br />
ellson@picoliterinc.com<br />
63<br />
Co-Author(s)<br />
Mitchell Mutz, Clifford Brown,<br />
Brent Browning, Richard Stearns,<br />
David Harris, Ma Phiengsai,<br />
Roeland Papen<br />
DMSO Hydration Monitoring by Ultrasound in Compound Library Microplates and<br />
Implications for Tracking Compound Dilution<br />
Dimethyl sulfoxide (DMSO), the most common solvent for storage of compound libraries in microplates, is highly<br />
hygroscopic. When exposed to the humidity of a normal laboratory atmosphere, the DMSO in each well absorbs<br />
water and thus dilutes the concentration of its compound. Ultrasonics provides a method for measuring the<br />
hydration level of DMSO in microplates with CV’s under 2% by detecting reflected ultrasonic waves from the<br />
bottom of microplate wells. No removal of test samples, addition of any chemical marker, or storage in clear or<br />
unsealed microplates is required. Results from a time-course study of hydration of a 384-well plate exposed to a<br />
humid laboratory atmosphere will be shown. By tracking hydration of DMSO in microplates, real-time predictions<br />
of library compound concentrations can be made. Implications for use of hydration-corrected compound<br />
concentration in better quantifying compound transfer and improving data quality in IC50 experiments will be<br />
discussed.<br />
PODIUM ABSTRACTS
12:00 pm Wednesday, February 4 High Throughput Screening – Analytical Room A2<br />
Rebecca Zangmeister<br />
National Institute of Standards and Technology<br />
100 Bureau Drive, MS 8362<br />
Gaithersburg, Maryland 20899<br />
razang@nist.gov<br />
Integration of Hydrogel Based Bioassays Into Microfluidic Channels<br />
64<br />
Co-Author(s)<br />
Michael J. Tarlov<br />
We previously reported a method for immobilizing single-stranded DNA (ss-DNA) probe molecules in<br />
polyacrylamide hydrogels within plastic microfluidic channels. Low concentrations of fluorescent-tagged ss-DNA<br />
targets hybridize with immobilized probes and are detected in the hydrogels. We aim to couple two novel bioassays<br />
with this basic technology. The first is a diagnostic DNA assay that does not require pre-labeling of the target strand<br />
prior to analysis. The assay is based on the displacement of a short sacrificial fluorescent-tagged indicator oligomer<br />
by a longer untagged target sequence as it is electrophoresed through a DNA-containing hydrogel plug immobilized<br />
in a microfluidic channel. The distinct advantage of this assay is the ability to detect non-labeled target DNA. The<br />
second is a lead sensitive assay, based on the response of catalytic DNA to Pb(II) ions. Our goal is to immobilize<br />
the enzyme strand sequence of the catalytic DNA duplex in a hydrogel plug immobilized in a microfluidic channel.<br />
Fluorescently tagged substrate strands are electrophoresed into the hydrogel plug where they hybridize with the<br />
immobilized enzyme strands, forming the catalytic DNA system. Electrophoretic migration of Pb(II) ions through the<br />
hydrogel plug results in catalytic cleavage of the substrate strand and release of the fluorescent-tagged sequence<br />
fragment that is detected in a second capture plug. Both assays feature enhanced sensitivity due to high loading of<br />
DNA probes in the hydrogel plugs, the spatially confined, directed mass transfer characteristics of the microfluidic<br />
channels, and the inherently low fluorescent background of the hydrogels.<br />
3:30 pm Wednesday, February 4 High Throughput Screening – Data Analysis and QC Room A2<br />
Anthony Carlo<br />
Pfizer<br />
Eastern Point Road<br />
Groton, Connecticut 06340<br />
Anthony_A_Carlo@groton.pfizer.com<br />
Evotec µHTS Data Analysis – Making the Most of the Multiparametric Readout<br />
This presentation will discuss our efforts towards improving hit detection in lead discovery by increasing the<br />
confidence of identifying true positives verses false positives/negatives in primary HTS data. Towards this goal, the<br />
talk will focus on the use of single molecule fluorescence detection provided by the Evotec OAI µHTS platform,<br />
which provides multi-parametric data readout from miniaturized assay formats and how this information is<br />
processed. These techniques will be applied across multiple HTS assays.
4:00 pm Wednesday, February 4 High Throughput Screening – Data Analysis and QC Room A2<br />
Hanspeter Gubler<br />
Novartis Institutes for BioMedical Research<br />
WSJ-350.E15<br />
Basel, CH-4002 Switzerland<br />
hanspeter.gubler@pharma.novartis.com<br />
65<br />
Co-Author(s)<br />
Michel Girod, Sigmar Dressler,<br />
Rochdi Bouhelal, Daniela Gabriel,<br />
Johannes Ottl, Kamal Azzaoui<br />
HTS Data Analysis in the Real World: Practical Experience With HTS Data Quality Assurance<br />
Systems and Recent Integration of the GeneData Screener Software<br />
The application of comprehensive quality control and sophisticated data correction algorithms to High Throughput<br />
Screening (HTS) data has a long standing history in the NIBR Lead Discovery Center (LDC). Fully automated inhouse<br />
systems are sifting through HTS raw data to check quality and to detect and correct many systematic<br />
errors. Some lacking aspects – most importantly interactive data visualization, comprehensive statistical analyses<br />
and possibilities for easy cross-assay investigations – have led NIBR to enter into a system development<br />
collaboration with GeneData in 2002.<br />
The GeneData “Screener” software is tightly integrated to the LDC HTS data processing systems. Standardized<br />
instrument raw data with necessary experimental context information are automatically entering the “Screener”<br />
system in a near “real time” fashion. Data are thus readily available to the scientists for analysis and – if<br />
necessary – correction of systematic patterns. Additional software modules are used for hit selection, including<br />
results from other assays, either historical or direct counter screens. The combination of advanced assay<br />
technology with the application of sophisticated quality control, error detection and data correction algorithms<br />
(quality assurance) lead to improvements in HTS efficiency. We demonstrate this with a few case studies from our<br />
HTS labs. In addition, we provide some further insight into the performance of the pattern correction algorithms by<br />
applying them to simulated data of known intrinsic activity – and error structure.<br />
4:30 pm Wednesday, February 4 High Throughput Screening – Data Analysis and QC Room A2<br />
Maneessha Altekar<br />
GlaxoSmithKline<br />
709 Swedeland Road, Mail Stop UW2110<br />
King of Prussia, Pennsylvania 19406-0939<br />
maneesha.2.altekar@gsk.com<br />
On-Line QC for High Throughput Screening<br />
Co-Author(s)<br />
Glenn Hofmann, Isabel Coma,<br />
Jesus Herranz, Liz Clark,<br />
Gavin Harper, Mark Lennon,<br />
Frances Stewart<br />
GlaxoSmithKline’s migration from the HTS laboratory to the HTS aut<strong>omation</strong> factory is expected to result in greater<br />
throughput for screening. Thus there is a need to put data analysis systems in place to monitor the quality of the<br />
screens in real time to ensure that any wastage of compounds, reagents and other materials is held to a minimum<br />
if things go wrong during a run. The on-line QC process has been developed to perform plate level calculations<br />
and determine the health of a plate or the screening run at any given time according to specified business rules.<br />
Initially, plate failures or run stoppages due to business rules will be rare as screeners gain experience with using<br />
the system and evaluate the sensitivity of the business rules being applied. The initial purpose of the system is<br />
diagnosis rather than remedy, with the screeners being informed of problems as they occur. The ultimate goal<br />
is to automate the system to provide feedback in to the screening process that will result in the robotic platform<br />
pausing or stopping the run as appropriate.<br />
PODIUM ABSTRACTS
5:00 pm Wednesday, February 4 High Throughput Screening – Data Analysis and QC Room A2<br />
John Elling<br />
Datect, LLC<br />
2935 Rodeo Park Drive East<br />
Santa Fe, New Mexico 87505<br />
elling@datect.com<br />
Finding and Correcting Systematic Bias in Array Experiments<br />
66<br />
Co-Author(s)<br />
Brian P. Kelley<br />
Whitehead Institute<br />
Before the results of array experiments can be analyzed, the measurements need to be validated, normalized,<br />
and possibly modified and corrected. The Whitehead Institute has shown that frequency analysis can be used to<br />
detect nonrandom spatial correlations in arrays of data that is expected to be random. This technology can be<br />
used to find systematic errors in data from microtiter plates and microarrays that may otherwise be overlooked<br />
when their patterns are obscured by the experimental signal or random noise. When a systematic error occurs<br />
in arrays that may also have legitimate correlated experimental responses, the spatial correlations are overlaid.<br />
Similarly, the effect of multiple systematic errors will be superimposed to create the observed spatial correlation.<br />
We have developed techniques to detect and discriminate multiple systematic biases that occur together in array<br />
data. With this capability, the software can be set up to ignore the acceptable correlations in the data while alerting<br />
the scientist to the appearance of unexpected and potentially problematic correlations. Identifying the source of<br />
a systematic spatial error can be used by scientists to both debug the experiment and to select an approach to<br />
normalizing the bias to correct the error.<br />
8:00 am Thursday, February 5 High Throughput Screening – Automated Design Room A2<br />
Larry DeLucas<br />
University of Alabama at Birmingham<br />
CBSE 206, 1530 3rd Avenue South<br />
Birmingham, Alabama 35294-4400<br />
delucas@cbse.uab.edu<br />
Efficient Protein Crystallization<br />
Co-Author(s)<br />
Terry Bray, Lisa Nagy, Debbie McCombs<br />
University of Alabama at Birmingham<br />
David Hamrick, Larry Cosenza,<br />
Diversified Scientific, Inc.<br />
Alexander Belgoviskiy, Brad Stoops, Arnon Chait<br />
ANALIZA, Inc.<br />
The high throughput production of diffraction-quality crystals remains a major obstacle in structural proteomics,<br />
with success rates rarely exceeding 15% for soluble proteins. The Center for Biophysical Sciences and<br />
Engineering, Diversified Scientific, Inc., and ANALIZA, Inc. present a unique and powerful approach for rapidly<br />
and efficiently determining optimum protein crystallization conditions. This involves the combination of three<br />
key technologies: (1) automated nano-crystallization, (2) incomplete factorial screening, and (3) a specifically<br />
designed neural net crystallization prediction program. This approach demonstrates promise for optimizing protein<br />
crystallization when combined with balanced sampling of crystallization space. This is accomplished using an<br />
incomplete factorial screen and a uniquely designed nano-crystallization system. Every crystallization trial outcome,<br />
including failures, is used to train the neural network. The self-organizing and predictive nature of the neural<br />
network allows for accurate prediction of previously untested crystallization conditions, even in the presence of<br />
noise. If properly trained, the neural network can be used to recognize important patterns of crystallization. This<br />
information allows the neural net to predict non-sampled complete factorial conditions used for optimization. Thus,<br />
it predicts the conditions that produce crystals from the entire “crystallization space” of possible experimental<br />
conditions, based upon results from a much smaller number of actual experiments performed. Therefore, a virtual<br />
screen can be performed using all possible combinations of components and variables. The top 50 scores for the<br />
predictive crystallization conditions are then experimentally prepared to determine/verify optimum crystallization<br />
conditions. Results from a statistically relevant protein sample number will be presented.
8:30 am Thursday, February 5 High Throughput Screening – Automated Design Room A2<br />
Normand Cloutier<br />
Bristol-Myers Squibb Co.<br />
5 Research Parkway<br />
Wallingford, Connecticut 06492<br />
normand.cloutier@bms.com<br />
67<br />
Co-Author(s)<br />
James Gill<br />
Jonathan O’Connell<br />
David Stock<br />
The Implementation of Statistical Design of Experiments (DOE) in the Construction of Assays<br />
and High Throughput Screens<br />
Many of the problems confronting scientists in the high throughput screening laboratory require optimizing a single<br />
result which is dependent on many interacting factors. In many industries these multi-factorial problems have<br />
long been solved using Statistical Design of Experiments (DOE). However we find that existing statistical design<br />
software packages are not sufficient for assay design. We have found that the specific challenges in the HTS<br />
laboratory require additional tools and efforts to effectively implement DOE methodologies. The adoption of DOE<br />
requires somewhat of a paradigm shift for many scientists. The promise of understanding a whole system in a<br />
fraction of the usual number of experiments is often met with a great deal of skepticism. Mistakes in the execution<br />
of the work can lead to erroneous conclusions that DOE does not work. At Bristol-Myers Squibb, we have found<br />
that general DOE training of scientists along with the establishment of reliable automated DOE processes have<br />
been effective in creating a positive and production experience. We have developed an automated DOE system,<br />
based on the TECAN Genesis workstation. We use the SAS JMP statistics package to generate experimental<br />
designs and in-house software to drive the robotics. We have successfully applied DOE to a broad range of<br />
problems confronting high throughput screening as whole. This talk will cover the process of automated DOE for<br />
assay design at Bristol-Myers Squibb. DOE successes, challenges in transferring this technology to experimenters,<br />
and lessons learned will also be presented<br />
9:00 am Thursday, February 5 High Throughput Screening – Automated Design Room A2<br />
W. Adam Hill<br />
Millennium Pharmaceuticals<br />
270 Albany Street<br />
Cambridge, Massachusetts 02139<br />
hill@mpi.com<br />
The Application of Design of Experiment in Developing Processes for Drug Discovery<br />
The statistical sampling which forms the basis for Design of Experiment has been used for almost a century,<br />
expanding from its early implementation in agriculture to more modern uses including aircraft and automobile<br />
design. Design of Experiment is now becoming accepted as a tool in drug discovery. Using DOE for developing<br />
crystallization conditions for proteins, developing biochemical assays as well as cell-based assays has led<br />
to the determination of robust procedures in much reduced timeframes. This presentation will focus on the<br />
implementation of DOE software and hardware across Millennium and its impact on specific projects.<br />
PODIUM ABSTRACTS
9:30 am Thursday, February 5 High Throughput Screening – Automated Design Room A2<br />
Amy Siu<br />
GlaxoSmithKline, Inc.<br />
5 Moore Drive<br />
Durham, North Carolina 27709<br />
amy.y.siu@gsk.com<br />
Automated Cell-based Assay Optimization by Design of Experiment<br />
68<br />
Co-Author(s)<br />
Jimmy Bruner, Deirdre Luttrell,<br />
Cathy Finlay, Mike Emptage,<br />
David Cooper<br />
The High Throughput Biology (HTB) department at GlaxoSmithKline implements statistical methods and<br />
procedures for developing and validating cell-based assays. Design of experiments (DOE) is a widely used<br />
and proven statistical method for optimizing experiments and processes. During phase I, the aut<strong>omation</strong> team<br />
in partnership with the statistics group did five DOEs to optimize the performance of a proprietary Erk MAPK<br />
Activation HitKit from Cellomics. In phase II, the team developed an in-house kit, using three DOEs. The<br />
GSK in-house kit was directly compared to the Cellomics Hitkits as optimized in phase I. The GSK kit had a<br />
larger window, lower background, and lower variability. Reagent costs for the two kits were also compared. This<br />
presentation describes experimental designs, aut<strong>omation</strong> programs, and statistical analysis of the data.<br />
3:00 pm Tuesday, February 3 Microfluidics Room A4<br />
Katherine Dunphy<br />
University of California, Berkeley<br />
6186 Etcheverry Hall<br />
Berkeley, California 94720-1740<br />
kadunphy@me.berkeley.edu<br />
Low-voltage, Spatially-localized Electrokinetic Control<br />
Co-Author(s)<br />
R. Karnik<br />
A. Majumdar<br />
Electrokinetic control is used in microfluidics as a method of choice due to ease of fabrication and lack of moving<br />
parts. Current work, however, relies on a single electric field generated by high voltages applied via electrodes at<br />
the channel ends. The high voltages necessary for current electrokinetic control require bulky and costly voltage<br />
sources, limiting microfluidics from becoming truly portable. In addition, spatially localized electric fields within the<br />
microchannel itself have not previously been accomplished due to the challenge of bubble formation (hydrolysis<br />
of water) at the electrodes. This work exploits electrochemical reactions at electrodes to create a device to have<br />
temporal and spatially localized electrokinetic control within a microchannel, accomplished with ±1V. This work<br />
introduces the use of silver-silver chloride electrodes within the microchannel to maintain an electric field. This<br />
work explains the use of Ag-AgCl electrodes to allow for temporal resolution and electric field programmability.<br />
By fabricating arrays of electrodes along the length of the microchannel, the electric field can be spatially<br />
controlled along the length of the channel. This work demonstrates electric fields comparable to those of gel<br />
electrophoresis, resulting in electrophoretic control of molecules in solution. These fields are controllable temporally<br />
as well as spatially, demonstrating new gains in electrokinetic control. The use of silver-silver chloride electrodes<br />
is an elegant, simple solution to a major challenge in microfluidic research. Development of such control allows<br />
microfluidics to move away from bulky, complex control to more compact, programmable, electrokinetic control.
3:30 pm Tuesday, February 3 Microfluidics Room A4<br />
Carl Meinhart<br />
University of California, Santa Barbara<br />
Santa Barbara, California 93106<br />
meinhart@engineering.ucsb.edu<br />
Analysis of Microscale Transport for BioMEMS<br />
During recent years there has been significant development in the field of microfluidics and its application to<br />
BioMEMS devices. The scalability and sensitivity of BioMEMS make them well suited for manipulating and<br />
analyzing macromolecules. Microfluidics plays a key role in the transport processes inside these devices,<br />
which include advection, Brownian motion, electrokinetic phenomena, and surface-dominated forces. Recent<br />
developments at UCSB of a fully-integrated tunable laser cavity sensor for optical immunoassays will be<br />
presented. This device incorporates a pair of Distributed Bragg Reflector (DBR) lasers to sense specific antigen/<br />
antibody binding events that occur in the evanescent field of the laser cavity. The binding event modifies the<br />
modal index of the laser through coupling of the evanescent field. The modal index can be detected theoretically<br />
to within a resolution of n ~ 10 -7 . Dielectrophoresis (DEP) is proposed as a method for manipulating the antigen<br />
concentration fields, thereby enhancing the sensitivity of the device. The length scales of microfluidic devices<br />
typically range between 100 – 102 microns. In order to make full use of the physical phenomena at this scale and<br />
to understand how these devices function, accurate non-intrusive diagnostic techniques are required. To this end,<br />
a micron-resolution Particle Image Velocimetry (micro-PIV) system has been developed to measure velocity-vector<br />
fields with order one-micron spatial resolution. The resolution of the PIV system is demonstrated by measuring the<br />
flow field in a 30 x 300 micron channel. By overlapping the interrogation spots by 50%, a velocity-vector spacing<br />
of 450 nm is achieved. Surprisingly, the velocity measurements indicate that the well-accepted no-slip boundary<br />
condition may not be valid for hydrophobic/hydrophilic boundaries at the microscale. These results represent the<br />
first direct experimental measurement of this phenomenon.<br />
4:00 pm Tuesday, February 3 Microfluidics Room A4<br />
Jill Baker<br />
Caliper Technologies Corp.<br />
605 Fairchild Drive<br />
Mountain View, California 94043<br />
jill.baker@calipertech.com<br />
Single Molecule Amplification in a Continuous Flow LabChip Device<br />
69<br />
Co-Author(s)<br />
Michelle L. Strachan, Ken Swartz,<br />
Yevgeny Yurkovetsky, Aaron Rulison,<br />
Carlton Brooks, Anne R. Kopf-Sill<br />
New biological and diagnostic markers are rapidly emerging from genomic studies that can provide meaningful<br />
insights into a patient’s health. In many cases, these involve the analysis of nucleic acids. For complex genomes,<br />
the polymerase chain reaction (PCR) is often used to prepare the sample for sequence-specific or allele-specific<br />
interrogation. The advantages of “lab-on-a-chip” devices, i.e., miniaturization, integration and aut<strong>omation</strong>, would<br />
be useful additions to the diagnostician’s arsenal as they produce better data quality, reduced cost, and improved<br />
ease-of-use features by comparison to conventional technology. An emerging need in nucleic acids diagnostics<br />
is detecting rare mutant molecules from conveniently obtained patient specimens that reflect the presence of<br />
neoplastic tissue. We have developed an automated, microfluidic system capable of analyzing single molecules<br />
by PCR amplification and TaqMan genotyping. The integrated features of this system make it a candidate format<br />
for high throughput, diagnostic laboratory settings. The system is unique in several respects. One, the system<br />
integrates reaction assembly, thermal cycling and fluorescence detection on one chip. This allows different<br />
samples to be tested one after another in an automated way, all at nanoliter scale. Two, the chip we have created<br />
uses our sip-and-split design and has eight channels in which eight different loci can be amplified at one time<br />
for each sample. In addition, we have recently configured the system to continuously amplify and detect single<br />
molecules of DNA.<br />
PODIUM ABSTRACTS
4:30 pm Tuesday, February 3 Microfluidics Room A4<br />
Rajiv Bharadwaj<br />
Stanford University<br />
Building 500, Chemical Engineering,<br />
Stanford, California 94305<br />
rajivb@stanford.edu<br />
A Generalized Dispersion Theory Model for Field Amplified Sample Stacking<br />
70<br />
Co-Author(s)<br />
Juan G. Santiago<br />
We will present an analytical model for concentration enhancement using field amplified sample stacking (FASS).<br />
This transient model is based on generalized dispersion theory and accounts for convective-diffusive transport<br />
of chemical species and electromigration. We model the FASS process as a one-dimensional electromigration<br />
and dispersion of two background electrolyte ions and one sample ion across an initial concentration gradient.<br />
Regular perturbation methods are used to solve for the concentration fields. The model has been validated<br />
using experiments performed in a microchannel system. We use an acidified poly(ethylene oxide) (PEO) coating<br />
to minimize dispersion due to EOF. Also, we use CCD-based full-field, quantitative, epi-fluorescence imaging to<br />
experimentally measure the unsteady concentration fields and validate the model.<br />
8:00 am Wednesday, February 4 Microfluidics – Separations Room A4<br />
Johan Vanderhoeven<br />
Free University of Brussels<br />
Pleinlaan 2<br />
Brussels, 1050 Belgium<br />
Johan.Vanderhoeven@vub.ac.be<br />
Co-Author(s)<br />
David Clicq, Kris Pappaert,<br />
Sarah Vankrunkelsven, Gino Baron,<br />
Gert Desmet<br />
On the Use of Nano-Channel Flows for the Enhancemant of Micro-Analytical Separations<br />
We will report on the use of 1D nano-channel flows for the enhancement of a wide range of different microanalytical<br />
separation techniques (liquid chromatography, DNA hybridization, protein binding kinetics measurement<br />
and the size separation and classification of particles and cells). To exploit the advantages (increased mass<br />
transfer rates and reduced solvent consumption and sample dilution) of miniaturised separation systems at their<br />
most extreme limit, we replaced the traditionally employed pressure-gradient and voltage-gradient flow driving<br />
techniques by a so-called shear-force driven method, enabling to establish high velocity flows through channels<br />
as thin as 50 nanometer, by simply mechanically moving the bottom part of the channel wall past the (stationary)<br />
upper part of the channel wall. With this novel flow driving principle, a wide variety of different fluid substances,<br />
ranging from small molecules, and going over proteins and large DNA coils to micron-sized particles, could be<br />
transported through silicon and fused silica etched nano-channels at velocities up to 5 cm/s. Examples of nanochannel<br />
separation applications such as ultra-rapid liquid chromatography separations of a 4-component coumarin<br />
dye mixture, enhanced DNA micro-array analysis, as well as a new separation method for the size separation of<br />
cells and nano-particles will be shown and discussed.
8:30 am Wednesday, February 4 Microfluidics – Separations Room A4<br />
H. John Crabtree<br />
Micralyne, Inc.<br />
1911-94th Street<br />
Edmonton, Alberta T6N 1E6 Canada<br />
john@micralyne.com<br />
Miniaturized Field Inversion Electrophoresis<br />
71<br />
Co-Author(s)<br />
L. M. Pilarski<br />
C. J. Backhouse<br />
Although significant strides have been made in achieving high resolution, microchip-based separations tend to<br />
involve separation distances of several centimetres and the application of several thousand volts. We have applied<br />
the field-inversion electrophoresis to enable the acquisition of high resolution data with very small separation<br />
distances (millimetres) and far lower voltages.<br />
9:00 am Wednesday, February 4 Microfluidics – Separations Room A4<br />
Steve Hobbs<br />
Nanostream<br />
580 Sierra Madre Villa<br />
Pasadena, California 91107<br />
steve.hobbs@nanostream.com<br />
Micro Parallel Liquid Chromatography System for High Throughput Analysis<br />
Pharmaceutical scientists increasingly seek applications of microfluidic technologies to miniaturize, multiplex, and<br />
automate experiments involved in biochemical analysis and high throughput screening. Nanostream has employed<br />
microfluidics to develop a system that enables micro parallel liquid chromatography (µPLC). This system was<br />
designed to match the functionality and performance of traditional LC instruments while offering an increase in<br />
sample analysis capacity. This increased analysis capacity translates to a significant savings in time for many<br />
applications throughout drug discovery and development. The first generation device incorporates 24 parallel<br />
microfluidic LC columns enabling 24 simultaneous, reverse-phase separations. Independent injection ports allow<br />
users to perform 24 unique separations in the time required to run a single sample on a conventional instrument.<br />
By minimizing sample and reagent consumption, mixed waste is significantly decreased. Additionally, the<br />
miniaturized platform reduces instrument footprint. This talk will provide an overview of the Veloce µPLC system<br />
instrumentation, e.g., autosampler and detection systems, and Brio cartridges. System performance, study<br />
duration, and reagent consumption will be compared to conventional techniques for selected applications.<br />
PODIUM ABSTRACTS
9:30 am Wednesday, February 4 Microfluidics – Separations Room A4<br />
Steven A. Soper<br />
Louisiana State University<br />
232 Choppin Hall<br />
Baton Rouge, Louisiana 70803-1807<br />
chsope@lsu.edu<br />
72<br />
Co-Author(s)<br />
Li Zhu, Rondedrick Sinville<br />
Louisiana State University<br />
Shelby Sutton, Gloria Thomas<br />
Mississippi State University<br />
Polymer-based Microfluidic Systems for the High Resolution Separation of Nucleic Acids:<br />
Applications in DNA Diagnostics and Sequencing<br />
We are preparing microchips for high efficiency separations of oligonucleotides with applications in both DNA<br />
sequencing and molecular diagnostics. To provide sufficient plate nu<strong>mbers</strong> for demanding separations, extended<br />
channel lengths (>10 cm) were used with the chips prepared in poly(methylmethacrylate) (PMMA). The chips were<br />
fabricated using hot-embossing from a Ni master, which was fabricated by UV-LiGA. In this presentation, two<br />
examples using these chips will be presented; (1) separation of ligase detection products prepared from genomic<br />
DNA possessing point mutations of high diagnostic value for colorectal cancers and; (2) DNA sequencing of gene<br />
conversion/replacement events between filled Alu chromosomal locations. K-ras genes from cell lines containing a<br />
single base mutation were PCR amplified and subjected to an allele-specific ligase detection reaction (LDR), which<br />
generated a product that was >50 bps in length. Using our microelectrophoresis chip, we were able to efficiently<br />
separate unligated from ligated primers at a wild-type to mutant ratio of 20 to 1 using a linear polyacrylamide<br />
(LPA, 3%) gel. For DNA sequencing, several issues demanded attention to achieve signal base resolution. The<br />
sequencing products required purification prior to electrophoretic sorting to improve loading onto the column.<br />
In addition, the relatively large electroosmotic mobility (EOF) associated with native PMMA required an EOF<br />
suppressant coating, accomplished by UV-activating the PMMA surface to create surface carboxylate groups,<br />
which were then coupled to methacrylic acid using EDC and subsequently polymerized to form an LPA coating.<br />
Using these chips, we will demonstrate reads that are comparable to glass chips of similar channel lengths.<br />
10:30 am Wednesday, February 4 Microfluidics Room A4<br />
Juan Santiago<br />
Stanford University<br />
Building 500<br />
Mechanical Engineering<br />
Stanford, California 94305<br />
juan.santiago@stanford.edu<br />
Electrokinetic Flow Instabilities<br />
Electrokinetics (EK) involves the interaction of solid surfaces, ionic solutions, and macroscopic electric fields.<br />
Applied electric fields can be used to generate bulk fluid motion (electroosmosis) and to drive the motion and<br />
separation of colloidal particles and molecules suspended in electrolyte solutions (electrophoresis). One important<br />
type of EK flows involves high conductivity gradients which may occur intentionally as in sample stacking<br />
processes, or unavoidably as in multi-dimensional assays or systems with poorly quantified samples. We have<br />
developed a physical model for this instability that captures the interactions between bulk liquid electric body<br />
forces, electromigration, viscous stresses, and convective diffusion. This work has impact on the general modeling<br />
of electrokinetic microfluidics and will provide important design guidelines for heterogeneous sample systems.<br />
Applications to sample stacking and rapid mixing will also be discussed.
11:00 am Wednesday, February 4 Microfluidics Room A4<br />
Christopher Tsu<br />
Millennium Pharmaceuticals<br />
270 Albany Street<br />
Cambridge, Massachusetts 02139<br />
tsu@mpi.com<br />
A Microfluidics Approach to Protease Substrate Identification<br />
73<br />
Co-Author(s)<br />
Larry Dick, Suresh Jain,<br />
Christine Martel, Benjamin Knight,<br />
Thomas Parsons, Michael Kuranda,<br />
Michael Pantoliano<br />
We describe the development of a novel fluorogenic protease assay for a chip-based microfluidics platform. We<br />
have tested several serine and cysteine proteases in validating the assay. We find the assay quantitative and<br />
equivalent in sensitivity to standard macrofluidic methods. We describe the screening of a discreet tripeptide<br />
AMC substrate library containing 6,000 individual compounds or 70% of the complete tripeptide sequence<br />
space of 8,800 compounds. Our microfluidics robot completed the initial screen in less than 12 hours with only<br />
10 micrograms of MTSP-1, a membrane-bound serine protease recently described by Craik and co-workers<br />
(Takeuchi, et al., J Biol Chem (2000) 275, 26333-42). Substrates of high activity were readily identified for MTSP-1<br />
and were consistent with published data. In collaboration with Caliper Technologies, a novel chip and software<br />
were created especially for this application. Recent tests of this exciting new design will be presented. We will also<br />
discuss the integration of microfluidics into other aspects of drug discovery, such as assay optimization and the<br />
potential for in vitro transcription translation (IVTT) as a starting point for HTS.<br />
11:30 am Wednesday, February 4 Microfluidics Room A4<br />
Daniel Chiu<br />
Cellectricon<br />
Fabriksgatan 7<br />
Gothenburg, SE 41250 Sweden<br />
daniel.chiu@cellectricon.se<br />
Microfluidic Chips for Time-Resolved High Throughput Electrophysiology<br />
Co-Author(s)<br />
Jon Sinclair, Johan Pihl,<br />
Jessica Olofsson, Mattias Karlsson,<br />
Cecilia Farre, Kent Jardemark,<br />
Owe Orwar<br />
We present a microfluidics-patch clamp platform for high throughput screening and rapid characterization of<br />
ion-channel-ligand interactions. An enabling microfluidics platform was developed that uses a plurality of parallel<br />
channels, having outlets ending in an open volume, in which the liquids in each channel couples viscously to the<br />
fluid stream exiting neighboring channels resulting in a collimation of flows. The solution environment around a<br />
stationary patch-clamped cell can be rapidly changed (exposure times down to ~1 ms) by scanning the outlets<br />
of the microfluidic channels across the cell at rates of 1-to-20 mm/s. Using this platform, kinetically resolved<br />
measurements and dose-response curves of hundreds of ligand solutions can be screened for in a single day.<br />
PODIUM ABSTRACTS
12:00 pm Wednesday, February 4 Microfluidics Room A4<br />
Helene Andersson<br />
Silex Microsystems<br />
Electrum 222<br />
Kista, 16440 Sweden<br />
helene.andersson@silex.se<br />
BIOMEMS Solutions at Silex Microsystems<br />
At Silex Microsystems we are applying micro-electro-mechanical-systems (MEMS) technology and manufacturing<br />
techniques to design and make components, such as sensors, actuators, and precision structures, for integration<br />
with customers’ end products. Miniaturization makes it possible to create life-saving applications that were<br />
impossible before. Lab-on-a-chip devices that conduct standard techniques such as electrophoresis, PCR and<br />
DNA sequencing have advanced significantly in the past few years. We’ve designed and manufactured a number<br />
of standard applications that can be used separately or together as a lab-on-a-chip. We have also created a<br />
number of devices for medical applications. For example, spiked electrodes for measuring bio-signals for Datex-<br />
Ohmeda. The individual spikes are designed to penetrate the human skin and are typically 40 µm wide and 150 µm<br />
high. The spike array is fabricated using deep reactive ion etching and is a promising alternative to standard<br />
electrodes in biomedical applications. For drug delivery applications we develop and manufacture a micropump<br />
for the company Debiotech in Switzerland. The pump chips are 6x10 mm and the pump demonstrates linear<br />
and accurate (±5%) pumping characteristics for flows up to 2 ml/h. We also develop chip-based solutions for<br />
high throughput ion-channel characterizations for Sophion Bioscience in Denmark. Silex is also manufacturing<br />
a pressure sensor that is used for measuring the blood pressure inside coronary arteries. The chip size is<br />
0.1x0.1x1.3 mm and is developed for a Swedish company called Radi Medical. In addition, we design and<br />
manufacture a variety of microfluidic devices for biotech applications.<br />
3:30 pm Wednesday, February 4 Microfluidics – Detection Room A4<br />
David Cliffel<br />
Vanderbilt University<br />
VU Station B Box 1822<br />
Nashville, Tennessee 37235-1822<br />
d.cliffel@vanderbilt.edu<br />
Automating a Multichamber, Multianalyte Microphysiometer for Metabolic Responses<br />
Using Labview<br />
74<br />
Co-Author(s)<br />
Sven Eklund<br />
Eduardo Lima<br />
John Wikswo<br />
Commercial microphysiometers have been limited to the analytical detection of acidification rate using a<br />
photoelectrochemical pH sensor. We have built a multianalyte microphysiometer by modification of a commercial<br />
Cytosensor instrument that detects multiple analytes involved in the metabolic physiology simultaneously.<br />
Monitoring the uptake of glucose and oxygen, and the production of lactate and acid gives a more complete<br />
picture of the metabolic processes within the cell than just acidification alone. Metabolic processes such as<br />
glycolysis, mitochondrial ATP generation, and glycogenesis are all directly related to the flux of these analytes.<br />
The metabolic response of sub-lethal concentrations of toxins has been measured. We have recently modified 2<br />
Cytosensor microphysiometers with independent multipotentiostat control for each chamber using Labview. This<br />
allows us to record 4 analytes simultaneously per chamber for all 4 or 8 cha<strong>mbers</strong>. Metabolic response analysis<br />
is performed in real-time as the raw electrochemical data is recorded. This talk will outline the modifications<br />
necessary, the calibration of the various sensors, biofouling problems of cells undergoing chemical stress, and<br />
the metabolic rate responses of Fibroblast and CHO cell lines to low concentrations of toxins and other agents<br />
including cyanide, fluoride, dinitrophenol, deoxyglucose, paraoxon, and antimycin. Temporal resolution of<br />
metabolic responses is much faster than conventional well-plate studies, leading to dynamic metabolic data. The<br />
generation of toxin dose response curves for multianalyte responses offers a big advantage to the detection and<br />
identification of toxins using cellular metabolic activity as the initial analytical sensing tool.
4:00 pm Wednesday, February 4 Microfluidics – Detection Room A4<br />
Cengiz S. Ozkan<br />
University of California, Riverside<br />
Bourns Hall, A305<br />
Riverside, California 92521<br />
cozkan@engr.ucr.edu<br />
Heterostructures of Nanomaterials and Organic-Inorganic Nanoassemblies<br />
Conventional nanofabrication strategies must be augmented by new techniques including self assembly methods<br />
in order to truly take advantage of the quantum nature of novel nanoscale devices and systems and permit<br />
the use of these properties for “real” applications in a larger system (> 10 nm and < 1 micron). In this talk, I will<br />
describe a novel technique for the fabrication of nano-assemblies of carbon nanotubes (CNT) and quantum dots<br />
(QD) – formation of CNT-QD conjugates. CNT’s are primarily functionalized with carboxylic end groups by oxidation<br />
in concentrated sulfuric acid. Thiol stabilized QD’s in aqueous solution with amino end groups were conjugated<br />
to carbon nanotubes using the ethylene carbodiimide coupling reaction. Next, I will discuss the possibilities of<br />
using carbon nanotubes for encapsulation and mass transport and present our first observations in this area.<br />
Fourier transform infrared spectroscopy data for the chemical modification of carbon nanotubes and scanning<br />
and transmission electron microscopy images of the nanobuilding blocks and the nanotube filling process will be<br />
presented. Potential applications of our studies include the fabrication of novel electronic and biophotonic devices,<br />
crystal displays and biosensors.<br />
4:30 pm Wednesday, February 4 Microfluidics – Detection Room A4<br />
Wayne Weimer<br />
US Detection Technologies<br />
2611 Internet Boulevard, Suite 109<br />
Frisco, Texas 75034<br />
wweimer@usdetect.com<br />
Surface Enhanced Raman Spectroscopy for Real World Samples<br />
Precise control of thermal evaporation deposition parameters allows the reproducible production of silver and gold<br />
island films on glass substrates with tunable surface plasmon resonance wavelengths. Specific combinations of<br />
substrate temperature, deposition rate, and film thickness produce films exhibiting surface plasmon resonance<br />
wavelengths that can be adjusted from throughout the visible and into the near infrared regions of the electromagnetic<br />
spectrum. The effects of deposition parameters on surface plasmon resonance wavelengths are quantified using a<br />
so-called “design of experiment” analysis. The analysis produces reliable predictive models for producing gold films<br />
with predetermined surface plasmon resonance wavelengths. Nonresonant enhancement factors in excess of eleven<br />
orders of magnitude for surface enhanced Raman spectroscopy were recorded for rhodamine 6G dye on optimally<br />
tuned gold films. Spectra were obtained from submonolayer samples, corresponding to the detection of at most<br />
180 attograms of the dye. Techniques for the adaptation of these surface plasmon resonance tunable gold films for<br />
detection of biological and explosive compounds will be discussed.<br />
75<br />
PODIUM ABSTRACTS
5:00 pm Wednesday, February 4 Microfluidics – Detection Room A4<br />
R. Scott Martin<br />
Saint Louis University<br />
3501 Laclede<br />
St. Louis, Missouri 63103<br />
martinrs@slu.edu<br />
Advances in Electrochemical Detection of Neurotransmitters in Microchannels<br />
76<br />
Co-Author(s)<br />
Michelle W. Li, Dana M. Spence,<br />
Nathan A. Lacher, Susan M. Lunte<br />
University of Kansas<br />
It has been shown that electrochemical (EC) detection is an attractive way to analyze neurotransmitters in small<br />
volume biological samples. In recent years, EC detection of neurotransmitters has also been accomplished in<br />
microfabricated devices, usually after separation by capillary electrophoresis (CE). In terms of microchip CEEC,<br />
the separation and detection performance (number of plates, peak skew, and detection limits) has, in general,<br />
been inferior to the most popular detection scheme, laser-induced fluorescence (LIF). Previous studies have<br />
shown this is attributed to the manner in which the electrophoretic voltage is decoupled from the potentiostat.<br />
This presentation will describe the fabrication of a fully integrated palladium decoupler that enables the working<br />
electrode to be isolated from the electrophoretic voltage while remaining in the separation channel. The optimum<br />
decoupler size and decoupler/working electrode spacing was determined using various buffer systems and<br />
field strengths to determine the amount of noise and resolution obtained for the separation of dopamine and<br />
epinephrine. LIF was used to study the amount of band broadening that results from the pressure-induced flow<br />
that occurs past the decoupler. These optimized designs were used for the separation of neurotransmitters with<br />
emphasis on a fully integrated PC-12 cell reactor/microchip CEEC analysis system that enables the separation<br />
and detection of dopamine and norepinephrine released upon Ca 2+ stimulation. Other related work pertaining to<br />
the development of a new method of fabricating carbon electrodes for EC detection in microchannels will also be<br />
described.<br />
8:00 am Thursday, February 5 Microchip – Separations Room A4<br />
Jörg P. Kutter<br />
Technical University of Denmark<br />
DTU, Building, 345 East<br />
Lyngby, DK-2800 Denmark<br />
jku@mic.dtu.dk<br />
Co-Author(s)<br />
Klaus B. Mogensen<br />
Omar Gustafsson<br />
Rikke P. H. Nikolajsen<br />
Capillary Electrochromatography Chip Featuring Sub-micron “Channels” and Integrated<br />
Waveguides<br />
CEC combines the selectivity of liquid chromatography with the efficiency of capillary electrophoresis (CE), and<br />
when miniaturizing CEC, further advantages such as higher efficiency, shorter analysis time, accurate injection<br />
of small volumes, potential for on-chip pre- and post-separation treatment and parallel analyses are gained. We<br />
present a microfluidic separation device for capillary electrochromatography (CEC) featuring integrated waveguides<br />
for optical detection and microfabricated monolithic structures in the separation channel for attaching different<br />
stationary phases. We used an approach similar to the one previously investigated by the Regnier group at Purdue<br />
University, who call the support COMOSS (collocated monolithic support structures). Regnier et al. obtained a<br />
minimum channel width of 1.5 micrometer in quartz. We have developed a process where the channel width<br />
is subsequently reduced by conformal deposition of glass, which enables a channel width of 0.5 micrometer.<br />
Earlier, we have presented a capillary electrophoresis device with monolithically integrated waveguides for<br />
optical detection. The fabrication has been simplified by etching both the optical and fluidic elements in the<br />
same processing step. The chip design allows for both fluorescence and UV/VIS absorbance detection through<br />
integrated waveguides. Also, on-chip gradient elution and various different stationary phases can be realized to<br />
further tune the separation properties. The talk will discuss fabrication issues as well as the performance of the<br />
chip for separations of neutral analytes such as explosives and polycyclic aromatic hydrocarbons, PAH’s.
8:30 am Thursday, February 5 Microchip – Separations Room A4<br />
Don L. DeVoe<br />
Calibrant BioSystems, Inc.<br />
7507 Standish Place<br />
Rockville, Maryland 20855<br />
ddev@calibrant.com<br />
2D PAGE on a Chip: Multidimensional Protein Separations via High Throughput Microfluidics<br />
An automated system based on a microfluidic analog of traditional 2D PAGE has been developed for ultra-high<br />
throughput protein analysis. For the analysis of complex protein mixtures, two-dimensional polyacrylamide gel<br />
electrophoresis (2D PAGE) remains the method of choice for separating more than thousands of proteins prior<br />
to mass spectrometry (MS), with proteins separated by charge and size in a 2D gel. Despite the selectivity and<br />
sensitivity of 2D PAGE, this technique as practiced today is the collection of manually intensive procedures.<br />
Casting of gels, application of samples, running of gels, staining of gels, post-separation gel manipulation, and MS<br />
interfacing are all time-consuming tasks prone to irreproducibility, significant sample loss, and poor quantitative<br />
accuracy. Thus, automated, high resolution, rapid, reproducible, and ultrasensitive 2D separation techniques<br />
are needed for large-scale analysis of proteins as well as differential display of protein expression. To this end, a<br />
microfluidic 2D protein separation platform combining isoelectric focusing and SDS gel electrophoresis will be<br />
presented. By combining traditional 2D PAGE separations in an automated, massively-parallel microfluidic chip,<br />
analysis cycles of minutes are now feasible, compared with hours for 2D-PAGE. The system integrates sample<br />
handling and multidimensional separations with laser-induced fluorescence detection, enabling high sensitivity and<br />
dynamic range with negligible analyte loss. An overview of the technology and overall system will be provided, and<br />
applications in differential display of protein expression will be presented. Integration of an efficient chip-to-MS<br />
interface for high throughput large-scale protein analysis will also be discussed.<br />
9:00 am Thursday, February 5 Microchip – Separations Room A4<br />
Stevan Jovanovich<br />
Silicon Valley Scientific<br />
4059 Clipper Court<br />
Fremont, California 94538<br />
stevan@svsci.com<br />
77<br />
Co-Author(s)<br />
Iuliu Blaga, David Rank,<br />
Norman Burns, William Gurske,<br />
Roger McIntosh<br />
High Throughput Preparation of Nanoscale Samples for Capillary Array Electrophoresis and a<br />
Microchip-based Analysis System<br />
New sample preparation and analysis technologies are required to reduce sample volumes and increase analysis<br />
throughput for DNA sequencing, genotyping, proteomics, and drug screening. At Silicon Valley Scientific, we are<br />
developing a microfluidics system, the NanoPrep System, to perform biochemical processing of 500 nL volumes<br />
in arrays of capillaries in cassettes. The NanoPrep System is robust for dye-primer and dye-terminator cycle<br />
sequencing, PCR, single-base extension, and other reactions. For DNA preparations, a template normalization<br />
process results in readlengths and success rates that are equivalent to or better than full-volume reactions.<br />
We describe both manual and automated versions of the NanoPrep System and their use in high throughput<br />
sequencing using TempliPhi and advanced sample cleanup methods for capillary array electrophoresis on the<br />
MegaBACE family of instruments and on a microchip-based analysis system.<br />
PODIUM ABSTRACTS
9:30 am Thursday, February 5 Microchip – Separations Room A4<br />
Nathan Lawrence<br />
Boston Biomedica, Inc.<br />
217 Perry Parkway<br />
Gaithersburg, Maryland 20877<br />
nlawrence@bbii.com<br />
78<br />
Co-Author(s)<br />
James Behnke, Feng Tao,<br />
Chunqin Li, Pinar Tuzmen,<br />
Bita Nakhai, Richard T. Schumacher<br />
A Comparison of Pressure Cycling Technology (PCT) to Standard Laboratory Techniques For<br />
The Release of Nucleic Acids and Proteins From A Variety of Difficult-to-lyse Sample Types<br />
Sample preparation is often a major bottleneck limiting rapid discoveries in such diverse fields as agriculture,<br />
environment, genetics, and drug development. To this end, a Pressure Cycling Technology-based sample<br />
preparation system (PCT SPS) was developed by Boston Biomedica, Inc. and introduced to the market in<br />
September 2002. This System uses an instrument (BarocyclerTM NEP2017) and single-use disposable processing<br />
tubes (PULSETM Tubes). The PCT SPS applies cyclic ambient to high hydrostatic pressure to disrupt tissues,<br />
cells and cellular structures, releasing their contents into buffers or other solutions contained in the PULSE Tube.<br />
The instrument is either manually or computer controlled, is capable of cycling pressure between ambient and<br />
40,000 PSI, and offers a working temperature range of 4 – 37˚C. Since its introduction, important new applications<br />
and discoveries have been made using the Barocycler NEP2017 in a number of fields, including genomics,<br />
proteomics and pharmacology. Data are shown for the release of nucleic acids and proteins from a wide variety<br />
of cells and tissues including microbes, plants, animals, and human tissue. A comparison of the efficiency,<br />
reproducibility, versatility, and ease-of-use of the PCT SPS to conventional sample preparation methods such as<br />
mortar and pestle, bead beating, homogenization, and sonication is presented. The released biomolecules were<br />
evaluated by a number of laboratory techniques, including gel electrophoresis, PCR amplification, and microarray<br />
analysis (nucleic acids), and 1D and 2D gels (proteins). Protein preparations were also assessed for the release of<br />
additional proteins and protein complexes compared to traditional protein preparation methods.<br />
10:30 am Thursday, February 5 Microfluidics – Bioanalytical Room A4<br />
Phillip Belgrader<br />
Microfluidic Systems, Inc.<br />
3918 B Valley Avenue<br />
Pleasanton, California 94566<br />
belgrader@mfsi.biz<br />
Co-Author(s)<br />
Nima Aflatooni, Kevin Brounstein,<br />
Bruce Johnson, M. Allen Northrup,<br />
Farzad Pourahmadi, Jennifer Velasco<br />
An Autonomous PCR-based Air Monitoring System to Detect and Identify Infectious Agents<br />
Continuous monitoring of the air for infectious agents has important and useful applications that include, protecting<br />
immunocompromised patients in hospitals, environmental surveillance of emerging and re-emerging outbreaks,<br />
monitoring for mold in sick buildings, homeland security, military force protection, and WMD arms control. We<br />
have assembled and tested a microfluidic-based system, called RAIDDS, that integrates air collection, sample<br />
processing, and PCR detection. Unlike other biological testing instruments that rely heavily on one-time use<br />
disposables, RAIDDS is modeled after chemical analyzers, which harbor reusable components. By reducing the<br />
operational cost of the instrument, widespread air monitoring for microbes will become feasible, practical, and<br />
beneficial. The core RAIDDS technologies include a sonicator for spore, cell, and virus lysis, silicon pillar chips for<br />
nucleic acid extraction, purification and concentration, and flow-through parallel PCR detectors. The complete<br />
system, including all peripheral hardware, is about the size of a water cooler and can run autonomously for days.
11:00 am Thursday, February 5 Microfluidics – Bioanalytical Room A4<br />
Adrian Winoto<br />
Caliper Technologies Corp.<br />
605 Fairchild Drive<br />
Mountain View, California 94043<br />
adrian.winoto@calipertech.com<br />
79<br />
Co-Author(s)<br />
Sherri Biondi, Andrea Chow,<br />
Bahram Fathollahi, Jim Mikkelsen,<br />
Michael Spaid, Ravi Vijayendren<br />
Protein Sizing and Relative Quantitation Determination Using a Microfluidic LabChip® Device<br />
SDS-Page has been the predominant protein sizing method for the past 30 years. This technique involves multiple<br />
manual operations including separation, staining, destaining and detection and typically requires several hours.<br />
We have developed a high throughput protein sizing assay which integrates each of these operations into a<br />
single microfluidic LabChip ® device. An assay is run by sipping unlabeled protein samples into the device using<br />
vacuum. The samples are then electrokinetically loaded and injected into the separation column which contains<br />
a low viscosity polymer sieving matrix. Both protein-SDS complexes and free SDS micelles are fluorescently<br />
stained during the separation process. Prior to detection, the sample is diluted to reduce the SDS concentration<br />
below its critical micelle concentration. This destaining step effectively reduces the background fluorescence<br />
from micelle-dye complexes so that protein-SDS-dye complexes can be detected. Using this technique we are<br />
able to size proteins between 14 and 200 kDa. The microfluidic device delivers a throughput of 75 seconds/<br />
sample with unattended sample sipping from a 96-well plate. In this presentation, we will show the experimental<br />
data describing the fundamental work to understand the assay performance. We will also describe the assay<br />
reproducibility in terms of sizing and mass quantitation. Finally, we will demonstrate assay performance with a<br />
broad range of customer sample types.<br />
11:30 am Thursday, February 5 Microfluidics – Bioanalytical Room A4<br />
Laurie Locascio<br />
National Institute of Standards and Technology<br />
100 Bureau Drive<br />
Gaithersburg, Maryland 20899-8394<br />
laurie.locascio@nist.gov<br />
Using Liposomes for High-Efficiency Mixing in Microfluidic Systems<br />
Co-Author(s)<br />
Wyatt Vreeland<br />
Andreas Jahn<br />
Michael Gaitan<br />
Liposomes have been used for many years in a variety of clinical and pharmaceutical applications related to<br />
drug encapsulation, targeting, and delivery. Currently, we are exploring analytical uses of liposomes focusing on<br />
their application in microfluidic systems as selective reagents for the performance of automated and targeted<br />
microchemical reactions. In this work, reagents are encapsulated inside the aqueous interior of liposomes<br />
that are dispersed in solution in a microfluidic channel. Reagent release is triggered through the modulation of<br />
temperature using an external heat source to locally change the solution temperature in the channel. The reagent<br />
release temperature is tunable based on liposome formulation; therefore, liposomes with different reagents can<br />
be programmed to sequentially release their contents thus enabling exquisite control of reaction timing. Because<br />
liposomes are evenly dispersed in the microchannel, reagent mixing in the microfluidic environment is very<br />
rapid upon release. We have also recently been exploring methods for the automated formation of liposomes in<br />
microfluidic systems. In this presentation, we will discuss several aspects of our research related to liposomes in<br />
microfluidic systems including the formation of liposome vesicles in microfluidic systems under various conditions;<br />
encapsulation efficiencies of different reagents inside liposomes made in microfluidic systems; and their application<br />
as reagents for automated microfluidic chemical reaction.<br />
PODIUM ABSTRACTS
1:30 pm Thursday, February 5 Microfluidics – Small Volume Dispensing Room A4<br />
Johan Nilsson<br />
Lund University<br />
Ole Romersvag 3<br />
Lund, SE-221 00 Sweden<br />
johan.nilsson@elmat.lth.se<br />
80<br />
Co-Author(s)<br />
Lars Wallman, Simon Ekström, Thomas Laurell<br />
Lund University<br />
György Marko-Varga<br />
Astrazeneca, Lund<br />
Proteomic Sample Preparation Using Piezo Dispensing and Capillary Force Driven Flow<br />
Despite the high sensitivity and relatively high tolerance for contaminants of matrix assisted laser desorption/<br />
ionization time of flight mass spectrometry (MALDI-TOF MS) there is often a need to purify and concentrate the<br />
sample solution, especially after in-gel digestion of proteins separated by two dimensional gel electrophoresis. A<br />
capillary force filling microsystem with a silicon microextraction chip or array and a piezoelectric microdispenser<br />
for sample clean-up and trace enrichment of peptides was manufactured and investigated. The micro extraction<br />
array was used to trap reversed phase chromatography media (Poros R2 beads) that facilitates the sample<br />
purification/enrichment of contaminated and dilute samples. The outlet of the extraction array was docked to the<br />
inlet of the capillary force filling microdispenser. 2 microliter of a matrix/elution solution that releases the peptides<br />
from the beads was applied at the inlet of the extraction array and fills both the extraction array and the dispenser<br />
by capillary force. The sample plug in the dispenser was deposited into a small spot on (< 400 micrometer) on the<br />
MALDI target increasing the sensitivity of the analysis. Using capillary forces only to fill the extraction array and the<br />
dispenser simplifies the operation of the system since there is no need for a tight connection between the matrix/<br />
elution solution supply and the inlet. The potential problem of trapping air bubbles in the dispenser during filling is<br />
also minimized.<br />
2:00 pm Thursday, February 5 Microfluidics – Small Volume Dispensing Room A4<br />
Donald Schwartz<br />
DRD<br />
83 Pine Street<br />
West Peabody, Massachusetts 01960<br />
donschwartz@drddiluter.com<br />
Co-Author(s)<br />
Donald S. Martin<br />
Differential Nano-Pipettor (NanoBlast): A Non-contact Pipettor That Handles Nanoliters for<br />
Plate Replication, Sample Transfers, Spotting, and Arrays<br />
Solid pistons, motor-driven smoothly and swiftly through seals, have been the mainstay of automated pipetting<br />
systems. However, the escalating demands to pipette low microliter or nanoliter volumes, contact free, are too<br />
severe for this classic design to meet because its single resolution mechanism cannot give both the fine aspiration<br />
resolution and high flow for sample blowoff that is needed. Hanging drops are common. An ever-morphing<br />
mélange of sensual robots cavorting with whackers, thwackers, pokers and zappers seeks to overcome this<br />
fundamental failing. The NanoBlast (unique and patent-pending) is a new miniaturized configuration of DRD’s<br />
Differential Displacement technology. Two pistons whose diameters differ by a few thousandths of an inch move<br />
together with respect to the same chamber (DRD Differential Mode). The very small cross sectional area difference<br />
gives extremely fine resolution, a robust 1.8 mm excursion/µL to call on to aspirate smoothly down to 50 – 100 nL<br />
even with disposable tips. Then only one piston moves (DRD Bulk Mode) and the abundant flow power (455 µL/<br />
inch) blows the minute sample out through a comfortable-sized orifice without damaging it (BlastoffTM) – to its<br />
microplate, array, spotting or MALDI target. No valves or auxiliaries are needed and there are no small seals. With<br />
conventional disposable plastic tips with IDs 0.016" – 0.020", the NanoBlast handled samples from 2 uL down<br />
to 170 nL (typical SD 30 – 35 nL). A fixed steel probe with tip ID 0.019" gives a SD of 20 nL. Disposable and fixed<br />
steel probes with tip IDs of 0.012" transfer proportionate-appearing drops down to 25 nL; quantitative studies of<br />
this are underway. The NanoBlast debuts as a bank of 8 and as a microplate-spaced block of 96, embodying<br />
the simplicity and smooth control of the classic pipettor with enormous additional range and reliability from its new<br />
DRD Differential Displacement power.
2:30 pm Thursday, February 5 Microfluidics – Small Volume Dispensing Room A4<br />
Terry D. Lee<br />
Beckman Research Institute of the City of Hope<br />
1500 E. Duarte Road<br />
Duarte, California 91010<br />
tdlee@coh.org<br />
An Electrochemical Pumping System for On-Chip Gradient Generation<br />
81<br />
Co-Author(s)<br />
Yunan Miao<br />
Beckman Research Institute of the City of Hope<br />
Jun Xie, Jason Shih, Qing He, Yu-Chong Tai<br />
California Institute of Technology<br />
Electrochemical actuation is unique in its ability to generate large displacements at high pressure while operating<br />
at a very low voltages. It is a viable alternative to the electrokinetic based approaches that are used in most<br />
microfluidic applications. We have developed a microscale, chip-based pumping system that is capable of<br />
delivering fluids over a range of flow rates (20–1000 nL/min) and pressures (0–200 psi). The devices described<br />
in this work were fabricated on the surface of a silicon wafer using a batch process that lends itself to mass<br />
production. The electrochemical pumps were integrated with other micromachined components on the chip<br />
including a low volume mixing chamber, a packed reverse phase column, and an electrospray ionization source.<br />
The work that will be presented represents significant progress toward a complete HPLC system on a chip.<br />
3:00 pm Thursday, February 5 Microfluidics – Small Volume Dispensing Room A4<br />
Thomas Corso<br />
Advion BioSciences, Inc.<br />
15 Catherwood Road<br />
Ithaca, New York 14850<br />
boardmaa@advion.com<br />
Fully Automated Nanoelectrospray With a BioMEMS Device<br />
Co-Author(s)<br />
Sheng Zhang<br />
Colleen Van Pelt<br />
Analytical sciences are moving toward miniaturization of chemical analysis systems. BioMEMS or Lab-on-a-chip<br />
systems offer advantages such as increased sample throughput, improved reproducibility, higher sensitivity, and<br />
significantly lower cost per sample over conventional, higher flow rate analyses. Although numerous microfluidic<br />
devices have been reported, applications rely primarily on spectroscopic detection in part due to the lack of a<br />
viable interface between microchip-based technology and mass spectrometry. Mass spectrometry is a powerful<br />
research tool for protein identification and more recently noncovalent interactions, however, current methods are<br />
labor intensive and low throughput. This presentation describes the first automated nanoelectrospray system for<br />
mass spectrometry, the NanoMate 100, allowing for automated analysis with low sample consumption. The<br />
technology is based on a fully monolithic microchip device, ESI Chip, containing an array of 10 x 10 single-use<br />
nanoelectrospray emitters. The microchip is fabricated from silicon substrates using deep reactive ion etching<br />
and other semiconductor techniques. The system eliminates carryover as each sample has its own disposable<br />
delivery tip and nanoelectrospray nozzle. Applications including protein-ligand noncovalent interaction studies and<br />
applications using on-line separation technology will be discussed.<br />
PODIUM ABSTRACTS
3:00 pm Tuesday, February 3 Proteomics – Arrays Room B1<br />
Brian Haab<br />
Van Andel Research Institute<br />
333 Bostwick<br />
Grand Rapids, Michigan 49503<br />
brian.haab@vai.org<br />
82<br />
Co-Author(s)<br />
Heping Zhou, Mark Schotanus, Randall Brand<br />
Evanston Northwestern Hospital<br />
Jorge Marrero<br />
University of Michigan Medical School<br />
Deborah Dillon, Jose Costa, Paul Lizardi<br />
Yale School of Medicine<br />
High Sensitivity Multiplexed Serum Protein Analysis Using Antibody Microarrays and<br />
Rolling Circle Amplification<br />
Antibody microarrays enable highly multiplexed and rapid protein measurements in low sample volumes. The<br />
profiling of proteins in sera and other bodily fluids using this tool should offer new opportunities for biomarker<br />
discovery and insights into disease biology. Robotically spotted microarrays of antibodies and proteins were used<br />
to measure the relative abundances of multiple proteins in serum samples from prostate cancer and pancreatic<br />
cancer patients and controls. Serum proteins that had been coupled to either a fluorescent tag (e.g., Cy3) or a<br />
hapten (e.g., biotin) were incubated on the microarrays, and specific proteins bound to the immobilized molecules<br />
on the microarrays through specific interactions. After washing away unbound proteins, bound proteins were<br />
detected using the fluorescent tag or amplified signal (using rolling circle amplification, RCA) from the haptenlabeled<br />
proteins. RCA significantly enhanced detection sensitivity while maintaining the accuracy and precision<br />
of the measurements. Measurements of dozens of proteins in sera from cancer patients and controls revealed<br />
significant differences in protein abundances between the two sample groups. The biological significance and<br />
potential clinical usefulness of the observed protein alterations in the sera of cancer patients will be discussed.<br />
3:30 pm Tuesday, February 3 Proteomics – Arrays Room B1<br />
Paul Predki<br />
Protometrix, Inc.<br />
688 E. Main Street<br />
Branford, Connecticut 06405<br />
paul.predki@protometrix.com<br />
Application of Functional Protein Microarrays to Kinase Drug R&D<br />
The ability to use protein microarrays to analyze protein function and interactions with a wide variety of molecules,<br />
including proteins, lipids, DNA, substrates, antibodies and drugs, has only recently been realized. Protometrix has<br />
industrialized and validated the complete process required for the development of proteome and sub-proteome<br />
microarrays for these applications. A yeast proteome array (the yeast ProtoArray) has already been completed,<br />
and a variety of human sub-proteome arrays are under development. Among these are human kinase arrays,<br />
which we have now validated for both interaction and activity assays. These simple protocols will provide powerful<br />
new capabilities when applied to the drug R&D process. Examples of these applications include kinase substrate<br />
identification, interaction and pathway mapping, antibody validation, inhibitor activity profiling and proteome-scale<br />
determination of inhibitor binding specificity. Recent results in each of these areas will be discussed.
4:00 pm Tuesday, February 3 Proteomics – Arrays Room B1<br />
Zheng Ouyang<br />
Purdue University<br />
560 Oval Drive<br />
West Lafayette, Indiana 47906<br />
ouyang@purdue.edu<br />
Protein Array Preparation by Ion Soft-Landing<br />
83<br />
Co-Author(s)<br />
Z. Takats, T. M. Blake,<br />
B. Gologan, J. M. Wiseman,<br />
J. C. Oliver, V. J. Davisson,<br />
R. G. Cooks<br />
A new method has been developed for preparing microarrays of biomolecules via soft-landing of mass selected,<br />
multiply-changed ions. A modified single quadrupole mass filter and a custom-built linear ion trap mass<br />
spectrometer have been used to select the ions and deposit them onto surfaces including: polycrystalline gold,<br />
functionalized self-assembled monolayers, and liquid thin films, at landing energies of 10-20 eV. The effectiveness<br />
of this method has been demonstrated via the preparation of a four-component array of proteins from a mixture<br />
of cytochrome C, apomyoglobin, lysozyme and insulin. The landed materials were recovered and analyzed by<br />
electrospray and MALDI mass spectrometry as well as being characterized in situ by laser desorption. Biological<br />
activity of the landed species was confirmed in the case of lysozyme, and in other experiments in the cases of<br />
trypsin, hexokinase and protein kinase A. The landing efficiencies were estimated to be a few tens of % and<br />
the spot sizes are ca. 1 mm in radius. The conditions for the soft-landing of proteins and peptides have been<br />
optimized. Neutralization of the ions on the surfaces was studied and both complete and incomplete neutralization<br />
were found, depending on the properties of the surfaces. The applications of this method to biology study and<br />
drug discovery as well as the modification of commercial mass spectrometers for soft-landing are discussed.<br />
4:30 pm Tuesday, February 3 Proteomics – Arrays Room B1<br />
Peter Wagner<br />
Zyomyx, Inc.<br />
26101 Research Road<br />
Hayward, California 94545<br />
pwagner@zyomyx.com<br />
New Detection Principles for Protein Biochips<br />
Protein biochips are becoming increasingly important for extracting comprehensive biological information<br />
from smallest sample volumes. Applications include the quantification of gene expression at the protein level,<br />
the measurement of protein-protein interactions, as well as functional protein activity. The diversity in protein<br />
measurements and assay variations requires different types of chip architectures and detection modes. Zyomyx is<br />
uniquely positioned to address the problems associated with micro-scale protein detection and characterization.<br />
Using proprietary biochip-based tools, Zyomyx has developed several novel platforms for proteomic research. An<br />
overview of protein biochip technologies will be presented with an emphasis on label-independent detection.<br />
PODIUM ABSTRACTS
8:00 am Wednesday, February 4 Proteomics – Structural Room B1<br />
Lance Stewart<br />
deCODE genetics, Inc.<br />
7869 N.E. Day Road West<br />
Bainbridge Island, Washington 98110<br />
lstewart@decode.com<br />
84<br />
Co-Author(s)<br />
Hidong Kim, Alexandrina Muntianu,<br />
Geetha Sundaram, Mark Mixon,<br />
Sattu Desai, Craig Sterling<br />
Advanced Mixology: Liquid Handling Devices for High Speed Cocktail Preparation and<br />
Iterative Protein-ligand Co-crystallization Experiments<br />
Early stage drug discovery programs can be greatly accelerated by the availability of high-resolution X-ray crystal<br />
structures of protein-ligand complexes. Ideally, multiple crystal structures of protein-ligand complexes are obtained<br />
within three to nine months after the initiation of a drug development program. Unfortunately, there are numerous<br />
bottlenecks between the selection of a target gene and the successful structure determination of a protein-ligand<br />
co-crystal. To address several bottlenecks in the crystallization process, we have developed an integrated system<br />
of secure database software and liquid handling robotics to automate the production, from stock solutions, of<br />
the thousands of formulations that are required for iterative screening and optimization of protein crystal growth.<br />
Protein-ligand co-crystallization screening is among the most demanding liquid handling applications in life<br />
sciences. Protein samples and chemical libraries are expensive, sensitive to degradation, and are typically only<br />
available in microgram to milligram quantities. Supply chain management of these materials is a challenge in and<br />
of itself, not to mention the demands for small volume non-contact dispensing of complex formulations that have<br />
varying viscosities, ionic strengths, chemical and pH gradients, etc. A typical crystallization experiment can often<br />
require the properly sequenced random access microshot delivery of five or more solution components from<br />
different source plates or vials (crystallization cocktail, protein, ligand, additive, reducing agent, metal co-factor,<br />
allosteric modulator, a second protein, etc.). Our efforts to address these demanding liquid handling issues will be<br />
described together with specific crystallization examples from our drug discovery programs.<br />
8:30 am Wednesday, February 4 Proteomics – Structural Room B1<br />
Frank Von Delft<br />
The Scripps Research Institute<br />
10550 North Torrey Pines Road-SR101<br />
La Jolla, California 92037<br />
loretta@scripps.edu<br />
High Throughput Technologies in Structural Biology and Applications Towards Genomes,<br />
Pathways, and Drug Design<br />
Four years after the start of the development of high throughput structural proteomics, we are now observing<br />
almost a dozen vendors manufacturing crystallization and imaging technologies. Significant improvements<br />
in the other areas related to the structural biology processes are also being seen. Integration and efficiency<br />
improvements based on data analysis remain as significant challenges, but new systems and approaches are<br />
emerging. Given this history, we are starting to observe the first sets of results that have directly come from these<br />
technological innovations. Lastly, both biotech and almost all me<strong>mbers</strong> of the pharmaceutical industry are now<br />
embracing high throughput structural proteomics technologies. A description of the current challenges and recent<br />
successes will be presented.
9:00 am Wednesday, February 4 Proteomics – Structural Room B1<br />
John A. Adams<br />
RoboDesign International, Inc.<br />
5120 Pasteur Court<br />
Carlsbad, California 92008<br />
jadams@robodesign.com<br />
Automated Protein Crystallization System<br />
85<br />
Co-Author(s)<br />
Janet M. Newman, David W. Jewell,<br />
John K. Hoffman, Mandel W. Mickley<br />
A complete, intermediate-sized (1100 plates), dual temperature, automated protein crystallization platform<br />
consisting of automated small-volume pipetting, automatic plate incubation, storage and retrieval, automatic drop<br />
imaging, database cataloging, analysis and classification, plus a closed-loop crystal trial experiment and trial<br />
optimization database that seamlessly connects all of the sub-system software and hardware. This system enables<br />
users to set up initial screens using standard screen blocks or to make custom initial screens, automatically make<br />
the experimental plates and have them transferred into incubation, imaged, analyzed, and classified based upon a<br />
user defined schedule. These results form the basis for an intelligent platform that can be used to more quickly and<br />
efficiently determine optimal protein crystal growth conditions.<br />
9:30 am Wednesday, February 4 Proteomics – Structural Room B1<br />
Brent Segelke<br />
Lawrence Livermore National Laboratory<br />
7000 East Avenue<br />
Livermore, California 94551<br />
segelke1@llnl.gov<br />
Automated Combinatorial Protein Crystallization Screening<br />
Co-Author(s)<br />
Dominique Toppani, Tim Lekin, Bernhard Rupp<br />
Lawrence Livermore National Laboratory<br />
Mary Cornett, Joel McComb<br />
Innovadyne Technologies, Inc.<br />
By considering crystal screening as a sampling problem, we have previously demonstrated, by probability theory,<br />
the inherent efficiency of stochastic combinatorial screening (Segelke, J. Crystal Growth 2000). While efficient in<br />
principal, stochastic combinatorial screening is difficult to automate in practice. Robotic liquid handling instruments<br />
are generally designed for high throughput mother-daughter transfers or for lower throughput, though versatile, rearraying.<br />
A new 96-tip, non-contact, liquid handling instrument by Innovdyne makes high throughput automated<br />
crystallization screening, on the fly, possible. The instrument is equipped with 96, independently actuated, noncontact,<br />
nano-dispensing tips. The Independent actuation enables any source any destination liquid handling.<br />
With a software experimental design engine, CRYSTOOL, aspirate/dispense operations are generated on the<br />
fly and passed to the instrument at run-time. Stock reagents arrayed in 96-well deep well blocks are aspirated<br />
simultaneously and dispensed in the random order and volume prescribed by worklists generated by the design<br />
engine. The instrument also maintains high precision over a broad range of volumes and viscosities, delivering<br />
exceptional versatility for types, concentrations, and ratios of components used in custom combinatorial screens.<br />
The same instrument can be used for rapid setup of vapor diffusion or microbatch crystallization experiments from<br />
premade screens or for the setup of grid optimization screens.<br />
PODIUM ABSTRACTS
10:30 am Wednesday, February 4 Proteomics – Informatics Room B1<br />
Eric Fung<br />
Ciphergen Biosystems<br />
6611 Dumbarton Circle<br />
Fremont, California 94555<br />
efung@ciphergen.com<br />
The Automated Biomarker System: A High Throughput Proteomics Biomarker<br />
Discovery Platform<br />
86<br />
Co-Author(s)<br />
Mark Garner<br />
The Automated Biomarker System is a proteomics platform that permits the rapid characterization of clinical<br />
samples and the generation of protein expression profiles for the discovery of biomarkers for disease. This<br />
platform consists of upfront automated liquid handling for sample preparation and ProteinChip Array binding. The<br />
bound ProteinChip Arrays are read in a ProteinChip Reader equipped with an autoloader, permitting arrays to be<br />
read unsupervised. This allows researchers to perform clinical proteomics programs in a more rapid manner than<br />
previously.<br />
11:00 am Wednesday, February 4 Proteomics – Informatics Room B1<br />
Xia Gao<br />
Structural GenomiX, Inc.<br />
10505 Roselle Street.<br />
San Diego, California 92121<br />
xia_gao@stromix.com<br />
Co-Author(s)<br />
Julie A. Reynes, Michelle Hartmann,<br />
Curtis Marsh, Ken Schwinn,<br />
Michael Sauder, Jeffrey B. Bonanno, Stephen K. Burley<br />
High Throughput Protein Domain Elucidation by Limited Proteolysis-Mass Spectrometry<br />
High-resolution structural information is crucial for understanding protein function in vivo and provides pivotal<br />
information for drug discovery. A typical approach to determining protein structure involves initial construct<br />
design, protein expression, purification, crystallization, and structure determination. This procedure is both time<br />
and labor intensive, and success is often dependent on making informed decisions at every stage, particularly the<br />
choice of protein domain boundary. Cohen et al. (1995) have demonstrated that protein functional domains can<br />
be accurately delimited by integration of proteolysis with mass spectrometry. Proteolytically defined domains are<br />
likely to produce diffraction quality crystals, as compact and well-folded domains often exhibit improved solubility<br />
and more highly ordered crystal lattice packing. We have conducted a critical evaluation of this method on a<br />
large number of structure determination target proteins produced for the New York Structural GenomiX Research<br />
Consortium (http://www.nysgxrc.org). The objectives of this study are to develop protocols for parallel proteolysis<br />
and automated data acquisition for multiple proteins, and to carry out a systematic study to correlate proteolysis<br />
defined domains with their success in crystallization. We designed an automated protocol to proteolyze eight<br />
proteins in parallel on a Tecan Genesis RSP100. The proteins were treated with four proteases (Trypsin, LysC,<br />
GluC, and ArgC) over a time course of six hours. Aliquots of each time points were prepared using the “thin-layer”<br />
technique for automated MALDI-MS data acquisition. After data interpretation, summary reports were generated<br />
in batch mode by Digests Reader developed at SGX, Inc. Protocols and interim results of these analyses will be<br />
presented.
11:30 am Wednesday, February 4 Proteomics – Informatics Room B1<br />
Jost Vielmetter<br />
Xencor<br />
111 W. Lemon Avenue<br />
Monrovia, California 91016<br />
jvielmet@xencor.com<br />
Protein Variation SAR Using ActivityBase<br />
87<br />
Co-Author(s)<br />
Richard Bishop<br />
Jeff Tischler<br />
Peter Cheung<br />
Protein therapeutics is a growing focus of discovery research organizations, but few informatics solutions exist<br />
for correlating protein structure with biological activity. Here we describe the first-ever ActivityBase application<br />
to provide protein structure-activity relationship (SAR) data. Xencor’s patented Protein Design Aut<strong>omation</strong> ®<br />
technology platform is the basis for structure-based protein engineering of therapeutic candidates to produce<br />
protein variants with optimized physico-chemical characteristics. These variants are registered in ActivityBase<br />
and subjected to both traditional HTS measurements, and virtual test occasions that extract the sequence<br />
variation information. Amino acids that differ from the reference sequence are recorded as test results, and<br />
their corresponding sequence positions as conditions. By storing both sequence variation identity and assay<br />
measurements as ActivityBase results, queries can be constructed that reveal the relationship between primary<br />
protein sequence and biological activity for any positional “condition.” This novel approach could potentially be<br />
used for the analysis of any string-based data set.<br />
12:00 pm Wednesday, February 4 Proteomics – Informatics Room B1<br />
Enal Razvi<br />
DiscoveRx Corporation<br />
42501 Albrae Street<br />
Fremont, California 94538<br />
erazvi@earthlink.net<br />
Strategic Opportunities in High-Content Screening and Computational Proteomics<br />
We present the industry landscape that is emerging in the computational proteomics space. This space is still in<br />
its infancy and for the most part undefined; therefore, we seek to present the market opportunity in informatics in<br />
the drug discovery space and then extend that to an examination of the industry trends in proteomics. In addition<br />
we will focus upon the qualitative and quantitative business opportunities in the high-content screening/secondary<br />
screening space; considered by many to be the next evolutionary step in computational proteomics. We will<br />
discuss strategic drivers and how these are translating into quantitative market opportunities for the vendors in this<br />
space. This presentation will include market models that are defining the evolution of this space.<br />
PODIUM ABSTRACTS
3:30 pm Wednesday, February 4 Proteomics – Technology 1 Room B1<br />
Judith Finlay<br />
Beckman Coulter<br />
7330 Carroll Road<br />
San Diego, California 92121<br />
jafinlay@beckman.com<br />
88<br />
Co-Author(s)<br />
Carlton Gasior, Chad Pittman, Melissa Rouzer,<br />
Graham Threadgill, Felix Montero<br />
Use of Beckman Coulter’s Biomek FX to Automate Epitope Discovery for Specific Antigens<br />
and Determine Optimal Peptides for Major Histocompatibility Complex (MHC) Class I Binding<br />
Stimulation of T cell response to specific antigens is an emerging technique used in vaccine discovery. The<br />
iTopia Epitope Discovery System from Beckman Coulter allows vaccine developers working on T cell mediated<br />
vaccines to map and characterize binding of epitopes to MHC complexes. The iTopia System can identify antigenspecific<br />
peptides that bind to any of eight different Class I MHC alleles, which represent approximately 90% of<br />
the human population. iTopia System uses microtiter plates coated with MHC Class I monomers to which certain<br />
peptides will bind in the presence of beta 2 microglobulin. Correctly folded complexes will be identified by binding<br />
of a fluorescent antibody, which recognizes only the properly folded tertiary complexes. iTopia System analyzes<br />
the binding, affinity and off-rates for the possible peptide/allele combinations of a selected protein. This speeds<br />
vaccine development by allowing a systematic ranking of candidate epitopes for subsequent functional studies.<br />
Aut<strong>omation</strong> of the liquid handling using the Biomek FX and data reduction are crucial to simplifying the iTopia<br />
process. A manual method was devised to carry out the screening. However, for an antigen of 120 kilodaltons,<br />
assuming screening 9-mer peptides, 992 peptides would need to be evaluated. It is estimated that 92 plates<br />
for each allele would be required to complete the iTopia process. Aut<strong>omation</strong> of iTopia will make it easier for<br />
companies working in vaccine discovery and development to incorporate this process into their workflow and to<br />
increase the efficiency and accuracy of this technology.<br />
4:00 pm Wednesday, February 4 Proteomics – Technology 1 Room B1<br />
Nenad Gajovic-Eichelmann<br />
Fraunhofer Institute Biomedical Engineering<br />
Arthur-Scheunert-Allee 114-116<br />
Bergholz-Rehbruecke, 14558 Germany<br />
nenad.gajovic@ibmt.fhg.de<br />
Co-Author(s)<br />
Eva Ehrentreich-Foerster<br />
Peter M. Schmidt<br />
Joerg Henkel<br />
Frank F. Bier<br />
Active Arrays – Time Resolved Analysis in Microarrays for Binding Kinetics and Enzymatic<br />
Activities<br />
The primary task of a microarray experiment is to detect a lot of binding events simultaneously. Most applications<br />
are transcription profiling and use fluorescence as label. Prior to the experiment, the sample has to be labeled by<br />
a suitable fluorochrome. The binding is done in a separate incubation step, the final result is taken after drying.<br />
Therefore usually microarray reader produce information on the fluorescence intensity at a given time of the<br />
binding process. Progress towards diagnostic applications is still slow, quantitation of the results is a problem<br />
and fabrication methods up to now are not reliable enough to allow for larg series production. The fluorescence<br />
intensity measured in microarray experiments represents the amount of bound analyte that depends on the<br />
concentration in the sample as well as on the affinity of the involved binding partners and time given for the<br />
binding. It is not possible to differentiate these influencing factors in the usual setup. To overcome the limitations in<br />
microarray technology developments are under way to facilitate measurement of binding kinetics in the microarray<br />
format. Homogeneous sample flow over the whole microarray is one of the technological problems that recently<br />
has been solved in our laboratory. Enzymatic reactions on immobilized substrates may also be observed using a<br />
flow through type of scanning device. Parallel detection and comparison of a variety of substrates or templates are<br />
now accessible in one single experiment and will be presented here. To demonstrate the power of the approach,<br />
we chose a restriction endonucleases.
4:30 pm Wednesday, February 4 Proteomics – Technology 1 Room B1<br />
Philip E. Dawson<br />
The Scripps Research Institute<br />
10550 N. Torrey Pines Road<br />
La Jolla, California 92037<br />
dawson@scripps.edu<br />
Probing Multicomponent Protein Assemblies Using Site-directed Attachment of<br />
Fluorophores and Crosslinking Agents<br />
89<br />
Co-Author(s)<br />
John H. Griffin<br />
Jose A. Fernandez<br />
Subramanian Yegneswaran<br />
Enzymes in the blood coagulation pathway are typically composed of a sequence specific peptide cleaving<br />
active site. However, much of the specificity and regulation of these enzymes is dictated by several protein<br />
binding surfaces (exosites) that mediate specific protein:protein interactions. For example, thrombin is specific<br />
for cleavage at Arg residues but gains specificity for cleavage of fibrinogen through interactions in exosite-1.<br />
Fibrinogen cleavage can be blocked by interaction with thrombomodulin that competes for binding to exosite-1.<br />
Similarly, the anitcoagulant leech peptide hirudin binds to the catalytic site and exosite-1 of thrombin. In order<br />
to better understand the relationship between active site and exosite binding between these serine proteases,<br />
we are developing methods to introduce a site specific, covalent label at either the active site or exosites in<br />
a manner that blocks binding and introduces a fluorophore and a chemical crosslinking agent. We have used<br />
this approach to unambiguously label the active site of the serine protease factor Xa with fluorescein and a<br />
benzophenone crosslinking agent. This enabled us to monitor the interaction of this protein with other components<br />
of the prothrombinase complex, an assembly of proteins that activates prothrombin to thrombin. Specifically, the<br />
binding of the substrate prothrombin to factor Xa was directly measured by both fluorescence anisotropy and by<br />
chemical crosslinking. In addition, this binding was increased 50-fold by the addition of the cofactor factor Va. We<br />
are applying this approach to the structural and functional characterization of the large multiprotein assemblies<br />
involved in blood coagulation.<br />
5:00 pm Wednesday, February 4 Proteomics – Technology 1 Room B1<br />
Travis Boone<br />
ACLARA BioSciences, Inc.<br />
1288 Pear Avenue<br />
Mountain View, California 94043<br />
tboone@aclara.com<br />
Co-Author(s)<br />
Tina Tian, Po-Ying Chan-Hui, Yuping Tan,<br />
Yining Shi,<br />
Hossein Salimi-Moosavi, Kathryn Stephens,<br />
Lilly Chen, Sharat Singh<br />
A Systems Biology Approach to Tracking Protein/Gene Expression and Interactions in<br />
Oncology and Toxicology Using the eTag Assay System<br />
The eTag Assay System enables the precise, simultaneous quantitation of tens of cellular pathway or clinical<br />
biomarkers across thousands of samples, providing high-value information about the expression and interaction<br />
of proteins and genes within cells. The eTag Assay System is a homogeneous method to analyze proteins and/<br />
or mRNAs directly from tissue samples, whole cells, or cell lysates. The multiplexing aspect of the technology is<br />
derived from the use of eTag reporters, which are fluorescent, biologically compatible labels that have distinct<br />
electrophoretic mobilities and can be separated from one another using capillary electrophoresis. The eTag<br />
reporters can be conjugated to a wide variety of biological probes including oligonucleotides, antibodies, proteins<br />
and peptides and are released in reaction as a consequence of a target-specific binding event. Mixtures of<br />
released eTag reporters are efficiently separated and sensitively detected using commercial capillary array DNA<br />
sequencing systems and accurately identified and quantified using proprietary, user-friendly software. The eTag<br />
Assay System has been employed in a systems biology approach to study gene and protein expression, cell<br />
signaling and pathway activation, and protein-protein interactions in oncology and toxicology applications. Results<br />
will be presented tracking receptor dimerization and signaling pathway phosphoproteins in breast cancer cell<br />
lines, as well as identifying markers in tissue samples. Data demonstrating simultaneous protein/gene profiling for<br />
toxicology screens on rat hepatocytes, across a set of drug compounds, will also be shown.<br />
PODIUM ABSTRACTS
8:00 am Thursday, February 5 Proteomics – Technology 2 Room B1<br />
David Goodlett<br />
ISB<br />
1441 North 34th Street<br />
Seattle, Washington 98103<br />
goodlett@systemsbiology.org<br />
The Finnigan LTQ-FT and Shotgun Proteomics<br />
Shotgun proteomics uses traditional protein and peptide separation methods, like size-exclusion chromatography<br />
(SEC) and strong-cation exchange chromatography (SCX), rather than gel electrophoresis to fractionate proteomes<br />
prior to mass spectrometric analysis. Because proteins are not purified prior to MS analysis the subsequent<br />
peptide mixtures can be quite complex. A number of groups have shown the advantages of Fourier transform<br />
ion cyclotron resonance (FTICR) MS for shotgun proteomic analysis. However, the instrumentation can be quite<br />
awkward for routine use making accurate mass measurements difficult to obtain during LC-ESI introduction.<br />
Recently, Finnigan introduced a new type of FTICRMS based, in part, on its successful and easy to use ion traps.<br />
This new FTICRMS, the LTQ-FT, offers high resolution and mass accuracy during LC-ESI introduction. In short<br />
the automatic gain control used to control ion population in the ion trap is used to control ion population in the<br />
ICR cell and allow good mass accuracy to 2 ppm during LC introduction. We will present an overview of shotgun<br />
proteomics, FTMS uses in the field and specifically review the advantages of this new type of instrument in the<br />
field of proteomics.<br />
8:30 am Thursday, February 5 Proteomics – Technology 2 Room B1<br />
Mary F. Lopez<br />
PerkinElmer Life and Analytical Sciences<br />
549 Albany Street<br />
Boston, Massachusetts 02118<br />
mary.lopez@proteomesystems.com<br />
90<br />
Co-Author(s)<br />
Alvydas Mikulskis, Richard Ediger,<br />
Eric Denoyer, Joseph DiCesare<br />
Rapid and Reproducible Fractionation and Identification of Proteins Using Membrane<br />
Chromatography and Orthogonal MALDI-TOF Mass Spectrometry<br />
Reducing the complexity of protein mixtures (such as those from whole cells) can facilitate the detection of low<br />
abundance, extremely acidic or basic, or very low molecular weight proteins. We have developed protocols for<br />
fractionation of numerous complex protein mixtures from human samples, including brain tissue, blood and<br />
cultured mammalian cells. Samples were fractionated in microscale on mini-columns or 96 well plates using<br />
novel membrane chromatographic substrates (VIVASCIENCE, Carlsbad, CA, USA) with various activated surfaces<br />
including ion-exchange, Cibachron Blue and others. After fractionation, proteins were identified with the PrOTOF, a<br />
novel orthogonal MALDI-TOF (PerkinElmer/MDS Sciex) mass spectrometer.
9:00 am Thursday, February 5 Proteomics – Technology 2 Room B1<br />
Wei-Wei Zhang<br />
GenWay Biotech, Inc.<br />
10130 Sorrento Valley Road, Suite C<br />
San Diego, California 92121<br />
wzhang@genwaybio.com<br />
Polyclonal Gene-Specific IgY Antibodies for Proteomics and Abundant Plasma<br />
Protein Depletion<br />
91<br />
Co-Author(s)<br />
Xiangming Fang<br />
Jerry Feitelson<br />
Lei Huang<br />
Kena Wang<br />
Polyclonal IgY antibodies are produced based upon gene information via immunizing chickens with gene<br />
expression vectors and/or purified protein antigens. Antibodies produced by this approach have high specificity<br />
and typically bind to a single gene product. Gene-specific IgY antibodies have several distinct advantages over<br />
conventional IgG antibodies due to their higher surface stability, lower cross-reactivity, stronger avidity, and<br />
higher binding capacity. Besides being useful in conventional immunoassays, IgY antibodies have demonstrated<br />
applicability for multiplex assays. In the Luminex-100 System and on Ciphergen’s ProteinChip, detection<br />
sensitivities reached 10 pg/ml and 40 fg/ml, respectively. To make detection of low-abundant proteins more<br />
effective and to facilitate human plasma proteomics studies, IgY antibodies are covalently conjugated to polymeric<br />
beads via Fc region for selectively removing abundant plasma proteins, such as albumin, IgG, transferrin, and<br />
fibrinogen. After short incubation of IgY beads with serum or plasma samples, target proteins are specifically<br />
bound to the IgY beads at an approximately 1:1 molar ratio. The flow-through samples can be used for proteomics<br />
analysis and for developing clinical diagnosis markers. This allows for highly sensitive analysis of rare proteins in<br />
blood samples using 2D gels and mass spectrometry. Also, polyclonal IgY against human serum albumin is shown<br />
to be capable of effectively removing the albumins from the serum samples of mouse, rat, pig, and goat. This<br />
cross-species binding of albumin cannot be accomplished by using IgG against the same antigen, indicating the<br />
unique and important feature of IgY in application for proteomics studies.<br />
9:30 am Thursday, February 5 Proteomics – Technology 2 Room B1<br />
Dhaval N. Gosalia<br />
University of Pennsylvania<br />
1150 Vagelos Labs<br />
3340 Smith Walk<br />
Philadelphia, Pennsylvania 19104<br />
dhavalg@seas.upenn.edu<br />
Protease Substrate Profiling Using Microarrays<br />
Co-Author(s)<br />
Scott L. Diamond,<br />
Cleo M. Salisbury,<br />
Jonathan A. Ellman<br />
University of California, Berkeley<br />
We developed a novel technique for microarray-based enzymatic assays for biological reactions at nanoliter<br />
volumes. The technique allows for rapid determination of protease substrate specificity with minimal sample<br />
usage. A peptidyl coumarin library of fluorogenic protease substrates, ACC-P1-P2-P3-P4-Ac was synthesized in<br />
parallel using standard Fmoc-based solid-phase synthesis techniques. The amino acids at P1 and P4 were held<br />
constant with P1=lysine and P4=alanine, and all combinations of proteinogenic amino acids (except cysteine)<br />
were used at the P2 and P3 positions, for a total of 361 compounds. Purity of the library was demonstrated<br />
by HPLC-MS, and the substrates were reconstituted in DMSO to a concentration of 10 mM. The library, along<br />
with appropriate controls, was further diluted to 1 mM concentration with 50% (v/v) glycerol and microarrayed<br />
on a standard microscope slide with a density of 400 spot/cm². The microarrayed library was treated with the<br />
serine protease human thrombin and then scanned. The chip demonstrated that thrombin had an extremely high<br />
specificity for proline at position P2. The remaining amino acids at the P2 position produced essentially no signal<br />
above background. Definite specificities were observed for amino acids at the P3 position with Q, V, W, Y, R<br />
resulting in the fastest cleavage and P3=D, P, A, H, E resulting in the slowest cleavage. The microarray specificities<br />
agreed with previously published results, demonstrating that the technique provides accurate substrate specificity<br />
information. This study indicates that protease substrate profiling can be done in this microarray format.<br />
PODIUM ABSTRACTS
10:30 am Thursday, February 5 Proteomics – Technology 3 Room B1<br />
Gary Schultz<br />
Advion BioSciences, Inc.<br />
30 Brown Road<br />
Ithaca, New York 14850<br />
gschultz@advion.com<br />
Sample Preparation Tips for Sample Enrichment and Direct Elution Nanoelectrospray<br />
Mass Spectrometry Analysis For Enhanced Sensitivity For Protein Characterization<br />
92<br />
Co-Author(s)<br />
Geoffrey Rule<br />
Sheng Zhang<br />
Colleen K. Van Pelt<br />
Amie Prince<br />
The sample diversity of proteomics requires analytical techniques that can provide rapid and sensitive<br />
characterization of complex biological systems. Nanoelectrospray mass spectrometry is one technique essential<br />
to every researcher working in this field due to its low sample consumption and sensitivity. NanoESI/MS provides<br />
long spray times useful for performing MS/MS including neutral loss and precursor ion scans. Combining<br />
NanoESI/MS with sample preparation can further improve sensitivity when sample enrichment or concentration<br />
is utilized. The NanoMate 100 with ESI Chip is an automated nanoelectrospray system developed to improve<br />
the efficiency and quality of NanoESI/MS. In operation, this system establishes an electric field localized at<br />
the exit of each microfabricated nozzle resulting in a stable, robust spray. That combined with the repeatable<br />
structure of the nozzles provides an automated platform for analysis of microliter sample volumes. Advantages<br />
of the system include low sample consumption, conservation of sample not consumed in the analysis, one-time<br />
spray optimization, enhanced spray stability, and no carryover. Sample preparation tips are routinely used for<br />
desalting and cleanup of proteins. The NanoMate functionality has been enhanced to enable the direct elution-<br />
NanoESI/MS of samples from sorbent-filled pipette tips. Low levels of peptides can be concentrated and enriched.<br />
Analytes are eluted in sub-microliter volumes at 100 nL/min delivering high analyte concentrations to the mass<br />
spectrometer. Applications of on-line elution will be demonstrated for protein digest analysis and identification of<br />
phosphopeptides and glycopeptides.<br />
11:00 am Thursday, February 5 Proteomics – Technology 3 Room B1<br />
Neil Kelleher<br />
University of Illinois<br />
600 S. Mathews Avenue<br />
Urbana, Illinois 61801<br />
kelleher@scs.uiuc.edu<br />
Progress in Automating Top Down Proteomics<br />
Co-Author(s)<br />
Steven Patrie, Dana Robinson,<br />
Yi Du, Lihua Jiang,<br />
Michael Roth<br />
An emergent “Top Down” approach to analysis of intact proteins will be described. Efforts in our laboratory<br />
combine informatics with a Quadrupole/Fourier-Transform hybrid mass spectrometer (Q-FTMS) to enable efficient<br />
characterization of biological events that change the mass of protein molecules from that predicted by an<br />
annotated genome sequence. A platform dedicated to Top Down is under development and uses a size-dependent<br />
proteome fractionation up front, followed by a Q-FTMS engine for data acquisition, and finally a custom database<br />
and software suite called “ProSight PTM” for streamlined protein identification and characterization (Anal. Chem.,<br />
2003, 75, 4081-4086). Protein examples from yeast and human cells will be described, including characterization<br />
of histone modifications using a new database strategy termed “prescriptive annotation”.
11:30 am Thursday, February 5 Proteomics – Technology 3 Room B1<br />
Joseph Loo<br />
University of California, Los Angeles<br />
405 Hilgard Avenue, 402 MBI<br />
Los Angeles, California 90095<br />
jloo@chem.ucla.edu<br />
A View of the Proteome Provided by New Mass Spectrometry Technologies<br />
93<br />
Co-Author(s)<br />
Rachel R. Ogorzalek Loo<br />
New technologies based on mass spectrometry (MS) and proteomics are being developed to address a number of<br />
health-related applications. Parallel approaches that probe complex systems as a unit, rather than one component<br />
at a time best allow for the understanding of protein expression per an organism’s developmental state and its<br />
response to the environment. An MS-based surface scanning method has been developed in which proteins are<br />
desorbed directly from 1D isoelectric focusing gels to create a virtual 2D gel; data are presented as an image,<br />
similar to that obtained from a stained 2D gel. Beyond the improved mass accuracy and mass resolution provided<br />
by substituting MS for SDS-PAGE, the virtual 2D gel has several advantages over the traditional 2D gel, including<br />
high-speed analysis. Small differences between measured and predicted molecular weights can flag contributing<br />
post-translational processing and protein modification. ESI-MS for studying noncovalent complexes has utility<br />
in chemical biology and biomedical research. ESI has the ability to ionize macromolecules and maintain weak<br />
noncovalent interactions. The mass measurement provides a direct determination of the stoichiometry of the<br />
binding partners in the complex. In addition to identifying the components that are involved in protein interaction<br />
networks, MS studies can identify and elucidate the geometry and interactions of protein machines, such as the<br />
690 kDa proteasome, the protease responsible for protein degradation, and the 14 MDa vault, a ribonucleoprotein<br />
particle implicated in multidrug resistance.<br />
1:30 pm Thursday, February 5 Aut<strong>omation</strong> Applications in Process R&D Room A2<br />
Norbert Stoll<br />
University Rostock<br />
R.-Wagner-Str. 31<br />
Rostock, 18119 Germany<br />
norbert.stoll@uni-rostock.de<br />
Microreactor Systems for Life Science Application<br />
Co-Author(s)<br />
Arne Allwardt<br />
Kerstin Thurow<br />
The use of combinatorial methods in chemistry and life science has been developing rapidly within the last years.<br />
Since there has been numerous developments in the field of pharmaceutical research in microtiter plate format<br />
there is still a lack of suitable instruments for classical. combinatorial chemistry in the mL-range. Synthesis in drug<br />
discovery or catalyst screening requires quite often high temperatures and high pressures as reaction condition for<br />
hydration, carbonylation or oxidation. The often long reaction times requires parallelization of reactors in order to<br />
increase the throughput. Existing instruments are not flexible automated solutions and do not have flexible material<br />
and information interfaces. On the other hand they do not always meet the requirements from organic chemists.<br />
To fill this gap an integrated automated system for organic high pressure synthesis had to be developed. The<br />
presentation will give an overview about existing systems and will show current and future developments in the<br />
field of organic high pressure synthesis. Two systems will be demonstrated for reactions in the mL-range (multi<br />
parallel reactor array 8/16/24) and for reactions in the MTP-Format (96 / 384). The systems are high temperature/<br />
high pressure designed. Both systems are integrated into an analytical environment to perform a coupling between<br />
synthesis and analysis. The 96/384 system can be operated stand alone or by a robot system.<br />
PODIUM ABSTRACTS
2:00 pm Thursday, February 5 Aut<strong>omation</strong> Applications in Process R&D Room A2<br />
Kara Rubin<br />
Merck Research Laboratories<br />
RY800-C210 P.O. Box 2000<br />
Rahway, New Jersey 08859<br />
Kara_Rubin@Merck.com<br />
Accelerating Polymorph and Salt Form Selection<br />
Crystallization of active pharmaceutical ingredients and their key intermediates has played an important role<br />
for pharmaceutical companies in getting a drug to market. Within Merck’s Process Research department<br />
crystallizations have been used in organic synthesis for various reasons, including the enhancement of chemical<br />
stability, resolution of enanitomers and purification. To accelerate this phase of the drug compound development<br />
process Merck entered into collaboration with Symyx Technologies to develop a High Throughput Polymorph<br />
and Salt Screening workflow. In this workflow, polymorph screens are performed to determine possible phase<br />
compositions, which are crystallized from various solvent combinations using a variety of procedures. Salt screens<br />
are performed on active drug molecules in the presence of various pharmaceutically acceptable bases or acids.<br />
The solids produced by the screens can be automatically analyzed by polarized light microscopy, X-ray powder<br />
diffraction, Raman spectroscopy, and melting point determination.<br />
2:30 pm Thursday, February 5 Aut<strong>omation</strong> Applications in Process R&D Room A2<br />
Brent Karcher<br />
Bristol-Myers Squibb Co.<br />
One Squibb Drive<br />
P.O. Box 0191<br />
New Brunswick, New Jersey 08903<br />
brent.karcher@bms.com<br />
MeDuSA, An Automated HPLC Screening Tool For PR&D<br />
94<br />
Co-Author(s)<br />
Merrill L. Davies<br />
Jack Venit<br />
Edward Delaney<br />
An automated tool for HPLC has been designed which enables process scientists to critically challenge currently<br />
utilized and/or accepted HPLC methods of analysis. Samples are subjected to a rigorous HPLC gauntlet<br />
comprised of an array of analytical reversed-phase columns possessing different selectivities, gradient elution<br />
using different eluents of various pH and solvent blends, with dual wavelength absorption detection. The resulting<br />
absorption data are presented as stacked chromatograms to provide a comprehensive picture of a sample’s<br />
impurity profile and a quick assessment of the best conditions to achieve chromatographic resolution of all<br />
sample components. The HPLC conditions are also amenable to both evaporative light scattering (ELS) and mass<br />
spectrometric (MS) detection. The entire package is controlled by custom software which streamlines sample<br />
queuing and HPLC control to afford a user-friendly “walk-up” system. The multi-dimensional screening and<br />
analysis (MeDuSA) concept has also been successfully extended to both reversed-phase chiral and normal-phase<br />
chiral HPLC. In addition, a mini-MeDuSA, utilizing shorter (50 mm) columns and higher flow rates, was developed<br />
to provide the analytics needed to support parallel experiments and high throughput screening.
3:00 pm Thursday, February 5 Aut<strong>omation</strong> Applications in Process R&D Room A2<br />
Rick Sidler<br />
Merck Research Laboratories<br />
R800-C260<br />
P.O. Box 2000<br />
Rahway, New Jersey 07065<br />
rick_sidler@merck.com<br />
Parallel Screening and Optimization of Catalytic High-Pressure Reactions<br />
The application of selective catalytic reactions for the preparation of pharmaceutically interesting compounds is a<br />
bourgeoning field. The variety of accessible reaction types, coupled with recently developed catalyst and ligand<br />
systems, challenge the researcher’s ability to screen and optimize these reactions. In addition, many of these<br />
interesting reactions employ conditions of high pressure, coupled with air and/or moisture sensitive catalysts<br />
to effect chemo-, diastereo-, or enantio-selective transformations. We have applied customized hardware and<br />
software solutions to allow for efficient screening, optimization, and analysis of these reactions.<br />
3:00 pm Tuesday, February 3 Genomics – Analytical Room A1<br />
Robert Cotter<br />
Johns Hopkins University<br />
725 North Wolfe Street<br />
Baltimore, Maryland 21205<br />
rcotter@jhmi.edu<br />
95<br />
Co-Author(s)<br />
Ben D. Gardner<br />
Sara McGrath<br />
Miniaturizing the Time-of-Flight Mass Spectrometer While Maintaining High Performance<br />
The time-of-flight mass spectrometer is unique in its ability to maintain high mass range when its size is reduced.<br />
Since sensitivity is also high, the major limitation in performance is mass resolution. In an approach developed in<br />
our laboratory a pulsed extraction, high order energy-focusing source is used to focus ions at the end of a three<br />
inch flight tube with mass resolutions up to 1200. In addition, a novel TOF mass spectrometer in which source and<br />
analyzer are combined in a single non-linear and time-dependent field has been developed. This instrument does<br />
not require extraction lenses or grids, and the electric field is defined by an “endcap” geometry. These instruments<br />
are currently being used for bioagent detection and proteomics studies.<br />
PODIUM ABSTRACTS
3:30 pm Tuesday, February 3 Genomics – Analytical Room A1<br />
Steven Hofstadler<br />
Ibis Therapeutics<br />
2292 Faraday Avenue<br />
Carlsbad, California 92008<br />
shofstad@isisph.com<br />
Detection and Characterization of Biothreats and Emerging Infectious Diseases –<br />
The TIGER Concept<br />
A new strategy has been developed to allow real-time tracking of bacterial or viral epidemics. Called Triangulation<br />
Identification for the Genetic Evaluation of Risk, or TIGER, the process is a rapid, recursive analysis that enables<br />
simultaneous identification of all pathogens present in a sample. The strategy relies on PCR amplification and<br />
base-composition analysis using high performance electrospray ionization mass spectrometry (ESI-MS). The<br />
base compositions from multiple primer pairs are used to “triangulate” the identity of the organisms present in<br />
the sample. Use of species-specific primers allows strain-typing of the organism. The utility of TIGER has been<br />
validated for both air sample monitoring against biowarfare agents and analysis of samples from Marine Corps<br />
recruits sickened in a Group A streptococcal (GAS) pneumonia outbreak. TIGER can be used to detect and<br />
identify infectious agents directly from a throat swab and hundreds of samples can be analyzed within 12 hours.<br />
This method allows real-time evaluation of patient samples and will make possible more rapid and appropriate<br />
treatment of patients in ongoing epidemic.<br />
4:00 pm Tuesday, February 3 Genomics – Analytical Room A1<br />
James Godsey<br />
Gen-Probe, Inc.<br />
10210 Genetic Center Drive<br />
San Diego, California 92121<br />
jimg@gen-probe.com<br />
The TIGRIS System for Total Aut<strong>omation</strong> of Nucleic Acid Testing<br />
The TIGRIS system developed by Gen-Probe for total aut<strong>omation</strong> of nucleic acid testing will be presented.<br />
96
4:30 pm Tuesday, February 3 Genomics – Analytical Room A1<br />
Mark Shannon<br />
Applied Biosystems<br />
850 Lincoln Centre Drive<br />
Foster City, California 94404<br />
shannome@appliedbiosystems.com<br />
Co-Author(s)<br />
Kathryn Hunkapiller, Julie Blake, Maria Roque-Biewer, David Ruff<br />
Applied Biosystems<br />
97<br />
Shashi Amur, Shirley Zhu, Vicky Seyfert-Margolis<br />
Immune Tolerance Network<br />
Multiplex Preamplification of Assays-on-Demand Product Targets Prior to Quantitative<br />
PCR Analysis of Gene Expression in Blood<br />
Real-time quantitative PCR (qPCR) is fast becoming an important alternative approach to microarrays for<br />
profiling the transcriptional states of cells and tissues. However, a requirement for several nanograms (ng) of<br />
cDNA per assay often limits the scale of such gene expression studies. To overcome this potential obstacle to<br />
large-scale expression studies, we have developed a method for preamplifying as many as 1000 Assays-on-<br />
Demand product targets in a single reaction prior to conventional quantitative PCR (qPCR). The method is<br />
highly reproducible, allows quantification of expression for hundreds of genes from as little as 0.01ng of cDNA per<br />
assay as starting material, and is close to 100% efficient for the vast majority of targets. We applied this method<br />
to expression profiling of 920 immune response related genes in T cells that were treated with phytohemagglutinin<br />
(PHA). For roughly 20% of the genes, expression was detected only with multiplex preamplification. Of these<br />
genes, approximately 60% were differentially expressed in response to treatment. This method is potentially<br />
applicable to other situations where starting material is limited such as laser-capture microdissected tissues,<br />
paraffin-embedded fixed tissues, and needle-biopsies.<br />
8:00 am Wednesday, February 4 Genomics – Instrumentation Room A1<br />
Steven Gordon<br />
Brooks-PRI Aut<strong>omation</strong><br />
15 Elizabeth Drive<br />
Chelmsford, Massachusetts 01824<br />
Parallab Technology: Integrated Nanoliter Genomic Workstation<br />
The Life Sciences Group of Brooks Aut<strong>omation</strong>, Inc. has developed a fully automated, integrated platform to<br />
perform nanoliter volume reactions. As an example, our standard DNA cycle sequencing reaction is done in a<br />
total volume of 500 nanoliters. A key element in this innovation is the proprietary Nano-Pipetter that incorporates<br />
96 glass capillary tubes that process all samples in parallel. The bench top Parallab 350 exploits the ability to<br />
aspirate small and accurate reagent volumes and subsequently completes all of the reaction and purification steps<br />
within the 96 miniature glass syringes. Each reagent is aspirated into the glass vessels, mixed, thermal cycled<br />
and purified before finally being dispensed into an output plate for analysis. After each sample set is complete,<br />
the Nano-Pipetter is decontaminated and reused, greatly reducing the number and cost of consumables normally<br />
associated with completing a similar sample set. For a genomics lab, the Parallab 350 is ideally suited for a<br />
range of applications, including cycle sequencing, PCR, SNP analysis, genotyping and end point analysis and can<br />
complete 1,800 samples per 24-hour day. We believe that this unique combination of attributes (nanoliter reaction<br />
volume, reduced consumable use and complete aut<strong>omation</strong>) offers a revolutionary approach to help accelerate<br />
discoveries in modern molecular biological laboratories.<br />
PODIUM ABSTRACTS
8:30 am Wednesday, February 4 Genomics – Genomics Instrumentation Room A1<br />
Jim Davis<br />
GenVault Corporation<br />
2101 Faraday Avenue<br />
Carlsbad, California 92008<br />
jdavis@genvault.com<br />
98<br />
Co-Author(s)<br />
Syrus Jaffe<br />
GenVault Corporation<br />
A Novel GenVault Multiwell Plate Format for Whatman FTA in Robotic, High Throughput<br />
DNA Archiving<br />
Tracey Long<br />
Whatman, Inc.<br />
A process has been developed using a unique 384 well, half-height microplate containing Whatman FTA ® to store,<br />
harvest and analyze DNA. This process eliminates the need for labor intensive manual punching and manipulation.<br />
The novel plate design uses 6 mm disks of FTA inserted into each of the 384 hourglass-shaped wells. Up to 12 µL<br />
of DNA containing fluid may be placed on each cup shaped FTA element. When desired, the sample is retrieved<br />
and the base and top seal of each well is easily pierced by a disposable rod that pushes the element into a<br />
collection tube or plate. Each element contains GenVault’s GenCode, a biological tracking agent that follows the<br />
DNA through the process and provides absolute sample tracking and identification at any stage in the process.<br />
Scaleable plate storage robotics are available for stable long-term room temperature archiving of DNA made<br />
possible by the unique properties of FTA. Archives for one thousand to over a million plates are available for use<br />
with this plate and media format. Processes have been generated for room temperature storage of dsDNA on FTA<br />
elements. Coupled with the ease of aut<strong>omation</strong> of this high storage efficiency plate design, this system enables<br />
large scale DNA archiving for genomics studies. DNA quality and purity and applications data will be presented.<br />
Information on the robotics design and operation and the associated software for tracking and searching samples<br />
will also be provided.<br />
9:00 am Wednesday, February 4 Genomics – Instrumentation Room A1<br />
Kurtis Guggenheimer<br />
University of British Columbia<br />
Room 325<br />
6224 Agricultural Road<br />
Vancouver, British Columbia, V6T 1Z1 Canada<br />
kurtis@physics.ubc.ca<br />
Electrostatic Printing of Composite Features for Highly Uniform DNA Microarrays<br />
Co-Author(s)<br />
Philip Dextras<br />
Andre Marziali<br />
Although present resolution limits of DNA microarray scanners would allow mechanically printed arrays to employ<br />
features as small as a few microns, variation in fluorescence ratios within each feature requires the acquisition of<br />
hundreds of pixels per feature to allow averaging and noise reduction, limiting the minimum useful feature size<br />
to 50 – 100 mm. This fluorescence variation results in part from non-linearity in the concentration dependence<br />
of dye fluorescence, and from variation in the printed oligo concentration within each feature. Mass transfer<br />
during evaporation and the crudeness of standard printing methods result in both severe concentration variation<br />
within the features, and large variations in feature morphology. Averaging to correct these variations can also<br />
lead to decreased accuracy in the quantitation of hybridized molecules through non-linear effects. We have<br />
developed a novel method for printing microarrays to ensure reproducible feature morphology and uniform DNA<br />
distribution within each feature. Features are printed by overlapping many smaller spots into the desired feature<br />
shape – much like letter printing in a desktop printer. Each spot-pixel is printed electrostatically, allowing rapid<br />
generation of 3 – 5 mm pixels without contact. This technique is expected to reduce fluorescence fluctuation<br />
within each feature – increasing data quality and allowing some reduction in feature size– and to improve feature<br />
morphology. In preliminary work, rectangular 50 mm features consisting of ten by ten grids of fluorescently<br />
tagged oligonucleotide spots have been printed and imaged both on an array scanner and a scanning electron<br />
microscope. Preliminary performance data from this printing method will be presented.
9:30 am Wednesday, February 4 Genomics – Instrumentation Room A1<br />
Ram Vairavan<br />
AutoGenomics<br />
2270-K Camino Vida Roble<br />
Carlsbad, California 92009<br />
rvairavan@autogenomics.com<br />
A New Dimension in Routine Genomic and Proteomic Analyses<br />
99<br />
Co-Author(s)<br />
Fareed Kureshy<br />
Vijay Mahant<br />
The INFINITI System is an automated multiplexing platform for genomic and proteomic analysis that delivers<br />
sample to results without manual intervention. Utilizing a novel thin film matrix as the microarray, disease<br />
specific probes are spotted on the surface and loaded into magazines. The INFINITI Analyzer integrates all<br />
the discrete processes of sample handling, reagent management, hybridization and detection for the analyses<br />
of DNA and proteins in a totally self-contained system. The “open architecture” design enables adaptation of<br />
multiple methodologies such as hybridization assay, primer extension assay, competitive and sandwich format<br />
immunoassays to perform Single Nucleotide Polymorphisms (SNPs), Short Tandem Repeats (STRs), micro-satellite<br />
analysis, gene expression analysis and protein determinations. The entire process is managed by the proprietary,<br />
Qmatic operating software which integrates all the complex processes in DNA analyses from amplified sample<br />
to test result without any operator intervention. All supplementary reagents are contained in the Intellipac reagent<br />
module with an on-board microchip that efficiently manages the reagents, electronically recording all relevant<br />
reagent specific information, such as expiry date, tracking reagent usage and the assay protocol for the specific<br />
test. The microarray is read in the built-in confocal microscope with results analyzed and presented in a graphical<br />
format for easy interpretation. The used chips are discarded in the waste drawer. The system can process 24<br />
microarrays simultaneously. Designed to operate on a “Load and Go” concept current applications include Cystic<br />
Fibrosis, Bleeding disorder tests, Th1/Th2 immune response test. Future applications include comprehensive<br />
profiles of specific cancers with DNA, protein and methylation markers.<br />
10:30 am Wednesday, February 4 Genomics – SNPs Room A1<br />
Keith Roby<br />
Beckman Coulter, Inc.<br />
4300 N. Harbor Boulevard<br />
Fullerton, California 92834<br />
kwroby@beckman.com<br />
Co-Author(s)<br />
Laura Pajak, Dana Campbell,<br />
Zhiming Jiang, Chad Pittman,<br />
Scott Boyer<br />
Aut<strong>omation</strong> of Amplification and Primer Extension Chemistries for Beckman Coulter’s<br />
SNPstream ® Genotyping System<br />
The information included in this presentation describes the utilization of the Biomek ® FX Laboratory Aut<strong>omation</strong><br />
Workstation for the process of SNP scoring using the SNPstream Genotyping System and assay reagents. Pre-<br />
and post-PCR methods are executed on separate instruments to ensure that the overall hygiene of the system is<br />
maintained thereby minimizing the possibility of cross-contamination. Pre-PCR methods include Genomic DNA<br />
Redistribution, and Multiplex Reaction Setup. Post-PCR methods include Exo/-SAP Cleanup, Multiplex Single<br />
Base Primer Extension and Hybridization of the tagged extension products to the SNPstream assay plate. The<br />
multiplex PCR and Primer extension protocols currently can accommodate up to 12 targets per reaction (i.e., a<br />
12-plex reaction). Components required to generate amplicons in a multiplex reaction and to process these targets<br />
with SNPware ® Reagent Kits will be described; this includes:<br />
• Describing the Biomek FX configurations utilized for pre- and post-PCR methods<br />
• Describing the important features of the methods used to generate and process samples<br />
• Describing results obtained when using these methods.<br />
Using the methodology described here, processing of 8 plates (>36,000 SNPs) can be achieved in approximately<br />
6 hours.<br />
PODIUM ABSTRACTS
11:00 am Wednesday, February 4 Genomics – SNPs Room A1<br />
Robert B. Cary<br />
Los Alamos National Laboratory<br />
P.O. Box 1663 MS M888<br />
Los Alamos, New Mexico 87545<br />
rbcary@lanl.gov<br />
100<br />
Co-Author(s)<br />
Hong Cai<br />
Richard T. Okinaka<br />
Paige E. Pardington<br />
Sensitive Detection and Identification of Threat Agents by Microarray-based Genotyping<br />
Using MLST Derived Signatures<br />
The rapid, accurate and sensitive detection of biological warfare agents requires a broad-spectrum assays capable<br />
of discriminating closely related microbial and viral pathogens. Moreover, in those cases where a biological agent<br />
release has been identified, forensic analysis demands detailed genetic signature data for accurate subspecies<br />
identification and attribution. Identification as well as the determination of important phenotypes, such as antibiotic<br />
resistance, can be accomplished by the examination of single nucleotide polymorphisms (SNPs) across multiple<br />
genetic loci. We have adapted a simple microarray-based genotyping assay to the detection of microbial pathogen<br />
signatures derived from multi-locus sequence typing (MLST) analyses. Using SNP signatures derived from MLST<br />
analysis of strains of Bacillus anthracis and its near neighbors we demonstrate that the assay can be used as a<br />
sensitive method for the detection and identification of Bacilli derived DNA while simultaneously providing accurate<br />
and detailed genotype information useful for subspecies identification. This work was funded by United States<br />
Department of Energy, CBNP funding to RBC. LA-UR-03-3343.<br />
11:30 am Wednesday, February 4 Genomics – SNPs Room A1<br />
Ming Xiao<br />
University of California, San Francisco<br />
Cardiovascular Research Institute<br />
505 Parnassus Avenue, Long 1329, Box 0130<br />
San Francisco, California 94143-0130<br />
mxiao@itsa.ucsf.edu<br />
Improvements on the TDI-FP SNP Genotyping Assay<br />
The TDI-FP (the template-directed dye-terminator incorporation assay with fluorescence polarization detection) is<br />
a homogeneous SNP genotyping assay. In the FP-TDI assay, the allele-specific dye terminators are incorporated<br />
onto an unlabeled SNP specific primer. The genotypes are inferred by the FP increase of dye terminators. FP<br />
detection works best when the reaction is driven into completion. The misincorporation of dye-terminators and<br />
the differential incorporation efficiency of two dye-terminators are the major reasons for the failure of the assay.<br />
We have optimized the assay by choosing two dye-terminators with equal incorporation efficiency and limiting the<br />
misincorporation.
12:00 pm Wednesday, February 4 Genomics – SNPs Room A1<br />
Tom Willis<br />
ParAllele BioScience, Inc.<br />
384 Oyster Point Boulevard Suite 8<br />
South San Francisco, California 94080<br />
tom@p-gene.com<br />
101<br />
Co-Author(s)<br />
Malek Faham, Paul Hardenbol,<br />
Maneesh Jain, Eugeni Namsaraev,<br />
George Karlin-Neumann, Hosssein Fakhrai Rad,<br />
Mostafa Ronaghi, Ronald W. Davis<br />
Comprehensive Genetic Analysis Using Highly Multiplexed SNP Discovery and Genotyping<br />
The elucidation of complex genetic traits increasingly necessitates comprehensive genetic analysis.<br />
Comprehensive coverage of the human genome is required in order to unlock the potential of association studies.<br />
At the same time allelic heterogeneity will necessitate comprehensive discovery of alleles within genes in order to<br />
capture the contribution of collections of rare alleles to the phenotype (e.g., BRCA mutations for breast cancer).<br />
New technologies are needed to address these fundamental challenges to human genetics. ParAllele BioScience<br />
has developed a set of technologies that enable such comprehensive coverage by providing a flexible platform<br />
that enables tens of thousands of interrogations to occur within a single tube reaction. The authors have used<br />
the concept of Molecular Inversion Probes (MIP) to build a SNP scoring technology that allows allele specific<br />
amplification of oligo probes through a process of self inversion. These molecular inversion probes can be<br />
multiplexed to the level of over 10,000 SNP calls per assay. Whole genome analysis is being enabled by this<br />
technology. In order to capture the important information from rare alleles and private mutation, a high throughput<br />
variation scanning technology has also been developed. This technology exploits the exquisite sensitivity of<br />
bacterial DNA repair to produce a multiplexed assay that allows thousands of DNA fragments to be sorted into<br />
those that contain mutations and those that do not. This technique, called Mismatch Repair Detection (MRD),<br />
allows comprehensive SNP discovery in patient populations. We have built a comprehensive genome analysis<br />
platform around these concepts by incorporating a molecular tagging strategy to allow robust detection of<br />
thousands of allele specific amplicons using a robust, generic chip platform. We believe that this technology will<br />
bring comprehensive association studies within reach by greatly reducing the cost and sample requirements of<br />
SNP discovery and genotyping. Multiplexed genotyping and SNP discovery results are discussed and performance<br />
metrics presented.<br />
3:30 pm Wednesday, February 4 Genomics – Informatics Room A1<br />
Terrence Smallmon<br />
LabVantage Solutions<br />
2355 Menard Street<br />
St. Louis, Missouri 63104<br />
tsmallmon@labvantage.com<br />
The Benefits of a Laboratory Information Management System (LIMS) in Genomics<br />
A genomic LIMS must streamline the data flow beyond the four walls of the laboratory. By centralizing all<br />
information about a sample, and relating it to other scientific information, scientists can improve productivity as<br />
well as the quality of information. The key benefits of a Genomics LIMS are:<br />
• Provides complete traceability of every sample.<br />
• Can acquire, integrate and manage multiple sources of dynamic and static data across the enterprise.<br />
• Reduces the amount of clerical work done by highly trained professionals – allows scientists to do more science.<br />
• Provides seamless integration with public/private databases.<br />
• Integrates with the solutions of your choice for scientific data interpretation and analysis.<br />
• Supports 21 CFR Part 11 Compliance.<br />
• Increases communication throughout the organization.<br />
• Reduces the number of times data is transcribed, increasing the integrity of the data.<br />
• Eliminates the need for human interaction with automatic features to generate routine reports.<br />
We now know as scientists expand the world’s understanding of complex biological systems, the volume of<br />
analytical data will continue to rise. This talk will discuss how the Genomics LIMS allows the researcher to manage<br />
this data and optimize their discovery process.<br />
PODIUM ABSTRACTS
4:00 pm Wednesday, February 4 Genomics – Informatics Room A1<br />
Andrei Verner<br />
McGill University and Genome Quebec Innovation Centre<br />
740, Doctor Penfield, #7506<br />
Montreal, Quebec, H3A 1A4 Canada<br />
andrei.verner@staff.mcgill.ca<br />
Feeding a Multiplatform Genome Center<br />
The McGill University and Genome Quebec Innovation Centre consists of 5 platforms that reflect the main<br />
directions in which genome research technology advanced in the last decade. These are: Genotyping, Sequencing,<br />
Chip, Proteomics, and Bioinformatics platforms. All the platforms function independently, but complement each<br />
other. The advantage of such architecture is that it allows for use of different approaches to tackle the same<br />
problem. The principle of mutually complementing approaches is also used within the Genotyping platform.<br />
Many novel methods of genotyping emerged recently, including those for Single Nucleotide Polymorphism (SNP)<br />
genotyping. In our Center SNP-genotyping may be done using 3 different methods. However, none of these SNPgenotyping<br />
techniques have become an obvious method-of-choice, mainly because of a big variety of incoming<br />
parameters. The best strategy depends on multiple factors, including the number of DNA samples, the number of<br />
SNPs to score, the turnaround time, etc. Thus, the old technological conflict between flexibility and speed became<br />
the problem of genomics. However, a combination of different techniques provides the recipe for success.<br />
4:30 pm Wednesday, February 4 Genomics – Informatics Room A1<br />
Roger McIntosh<br />
Silicon Valley Scientific<br />
P.O. Box 66749<br />
Scotts Valley, California 95067<br />
roger@mcintosh.com<br />
Open Interfacing for Lab Aut<strong>omation</strong><br />
This talk describes the design issues and development challenges during the three year development and<br />
deployment of a comprehensive lab aut<strong>omation</strong> software platform that uses XML and web services as its data<br />
interchange mechanism. Alternate data exchange formats, including SOAP, CORBA, and DCOM, will be discussed.<br />
Contrasts and comparisons of our approach with such alternate data interchange mechanisms, standardized<br />
data formats, and open interfaces will be discussed. This talk should provide significant insight to any group<br />
contemplating design and adoption of XML or web service based aut<strong>omation</strong> standards in the lab, particularly for<br />
robot and instrument aut<strong>omation</strong>. Scheduling and control issues will also be discussed.<br />
102
5:00 pm Wednesday, February 4 Genomics – Informatics Room A1<br />
Dave Riling<br />
DataCentric Aut<strong>omation</strong><br />
2525 Perimeter Place Drive, Suite 131<br />
Nashville, Tennessee 37214<br />
dave.riling@dcacorp.com<br />
The Changing Face of Lab Aut<strong>omation</strong><br />
103<br />
Co-Author(s)<br />
Greg Nordstrom<br />
DataCentric Aut<strong>omation</strong><br />
Onkar Singh<br />
GlaxoSmithKline<br />
As technology evolves, aut<strong>omation</strong> equipment becomes more and more capable and flexible. With this growing<br />
wealth of capability the demand by the scientist to conduct experiments faster, easier, and with higher reliability<br />
is also growing. This duality is creating a new paradigm in laboratory aut<strong>omation</strong>, “Solution Based Systems”.<br />
The traditional laboratory piece wise robotics with a non-coupled LIMS environment is rapidly being replaced<br />
with systems designed to incorporate various pieces of equipment to solve specific problems such as High<br />
Throughput Screen Systems or Automated Protein Crystallization Systems, but this merely marks the beginning<br />
of a broader shift in laboratory aut<strong>omation</strong> trends. Innovations in lab aut<strong>omation</strong> systems continue at a rapid<br />
rate thus today’s state of art is tomorrow’s closet junk. In order to break this cycle and provide workable cost<br />
effective solutions for the drug discovery arena a radical approach is emerging, Highly Adaptive Integrated Solution<br />
Environments which strongly couple the ease of experiment modification with comprehensive data collection and<br />
analysis methods to integrated lab aut<strong>omation</strong> solution based systems. This talk will focus on this emerging trend,<br />
discuss the advantages of this new environment for both scientist and engineer, and describe how to get your lab<br />
ready to push the envelope without large upfront costs or long-term paralysis in selecting new and replacement<br />
equipment. This talk will conclude with a discussion on how this new trend will benefit the scientist through<br />
automated optimization algorithms which are described in an actual implementation of a hybrid Automated Protein<br />
Crystallization System.<br />
8:00 am Thursday, February 5 Genomics – Arrays Room A1<br />
J. Colin Cox<br />
University of Texas<br />
1 University Station, A4800<br />
Austin, Texas 78712-0159<br />
j.colin.cox@mail.utexas.edu<br />
Co-Author(s)<br />
Travis S. Bayer<br />
Jim Collett<br />
Andrew D. Ellington<br />
Automated Aptamer Selection: Applications in RNA: Protein Sequence Specificity, and<br />
Aptamer-chip Microarrays<br />
In vitro selection and evolution is incredibly adept at producing nucleic acid binding species (aptamers). Like<br />
antibodies, these nucleic acid aptamers typically interact with their ligands with exceptionally high specificity and<br />
affinity. Their potential utility become even more evident when one considers the astonishingly large range of target<br />
ligands. Aptamers have been generated to bind ligands ranging from small ions to rat tails. Nucleic acid species<br />
can be manually evolved in significantly less time than required for antibody production. Regardless, typical<br />
selections can take upwards of weeks to a few months to finish. Our laboratory has pioneered the process of<br />
automating in vitro selection, facilitating the creation of aptamers in mere days. This ability bodes well for the rapid<br />
development of both large-scale aptamer-based evolutionary specificity studies as well as nucleic acid-based<br />
biosensor arrays. Presented herein are examples of these two branches of aptamer employment. Aptamers are<br />
uniquely suited for determining sequence recognition motifs of nucleic-acid binding proteins. We have explored the<br />
natural diversity of RNA binding peptides and proteins by investigating arginine-rich RNA binding motifs (ARMs)<br />
from eukaryotes, prokaryotes, and viruses that infect both. In addition, we have engineered several mutations<br />
of the human spliceosomal U1A protein and evolved aptamers to each mutant in order to elucidate nucleic-acid<br />
sequence:protein sequence binding “rules”. Finally, we have created novel aptamer-array chips that function<br />
similarly to traditional DNA-array chips. Aptamers on chips bind ligands as they would in solution, and these<br />
biosensing chips allow for the parallel measurement of multiple aptamer-ligand binding investigations.<br />
PODIUM ABSTRACTS
8:30 am Thursday, February 5 Genomics – Arrays Room A1<br />
Jon Chudyk<br />
Marshfield Clinic Research Foundation<br />
1000 N. Oak Avenue ML7<br />
Marshfield, Wisconsin 54449<br />
chudykj@cmg.mfldclin.edu<br />
Co-Author(s)<br />
Terry L. Rusch, Kim Fieweger, William Dickinson, Jian Che<br />
Marshfield Clinic Research Foundation<br />
104<br />
Mitchel J. Doktycz, Adong Yu, James L. Weber<br />
Oak Ridge National Laboratory<br />
Microtape and Associated Instrumentation for Continuous Array High Throughput<br />
Genotyping<br />
Lower cost, higher throughput genotyping is a common goal among genetics labs. Aut<strong>omation</strong> has greatly<br />
increased over the years, but still requires the manipulation of numerous microtiter plates. Our laboratory is using<br />
a continuous reel of 384 well arrays on polypropylene tape (microtape) to genotype diallelic polymorphisms.<br />
Genotyping costs are lowered through more efficient aut<strong>omation</strong> of the microtape as compared with microtiter<br />
plate handling, as well as a reduction in reagent costs by decreasing reaction volumes. Our current version of<br />
microtape has wells with reaction volumes of 700 nanoliters or less. The diallelic polymorphisms are typed by<br />
tagging allele-specific PCR primers with FAM or JOE molecular beacon uniprimers. Both short insertion/deletion<br />
and base substitution (SNP) polymorphisms have been successfully typed in the microtape using this assay.<br />
Commercial equipment for the microtape is not presently available, therefore a series of instruments to handle the<br />
tape were developed in-house. A pipetting instrument was developed to deliver specific DNA samples or other<br />
reagents. A solenoid micro-valve aspirating and jetting unit was developed for dispensing a common reaction<br />
mix. The arrays are sealed with commercially available continuous roll seal material prior to polymerase chain<br />
reaction (PCR). PCR is performed in a waterbath-based thermal cycler. After PCR the arrays are read using<br />
an epi-fluorescence detection unit. The reader uses either an argon ion or solid state laser for excitation and<br />
photomultiplier tubes (PMTs) for detection. The results are analyzed using software written in-house.<br />
9:00 am Thursday, February 5 Genomics – Arrays Room A1<br />
Andrey Ghindilis<br />
CombiMatrix Corporation<br />
6500 Harbour Heights Parkway Suite 301<br />
Mukilteo, Washington 98275<br />
aghindilis@combimatrix.com<br />
Immunoassays and Sequence-Specific DNA Detection on a Microchip Using Enzyme<br />
Amplified Electrochemical Detection<br />
Co-Author(s)<br />
Kilion Dill<br />
Kevin Schwarzkopf<br />
A CMOS fabricated silicon microchip was used as a platform for immunoassays and gene expression studies<br />
utilizing CombiMatrix’s unique DNA synthesis methodology. The chip is covered with a biofriendly matrix wherein<br />
the chemistries occur. The active silicon chip has over 1,000 active electrodes that can be individually addressed<br />
for both synthesis of DNA and protein attachment to a membrane on the chip surface. The active chip can be<br />
further used for the analysis of gene expression targets and detection of various analytes using antibody selfassembly<br />
immunoassay techniques.
9:30 am Thursday, February 5 Genomics – Arrays Room A1<br />
Frank F. Bier<br />
Fraunhofer Institute for Biomedical Engineering<br />
Arthur-Scheunert-Allee 114-116<br />
Bergholz-Rehbruecke, 14558 Germany<br />
frank.bier@ibmt.fhg.de<br />
105<br />
Co-Author(s)<br />
Ralph Hölzel, Alexander Christmann,<br />
Nenad Gajovic-Eichelmann, Joerg Henkel,<br />
Markus von Nickisch-Rosenegk<br />
Nanoarrays – A Bottom-up Method to Create Nanometer Addressable Lateral Surface<br />
Structures by use of Nucleic Acids<br />
For many applications manufactured surfaces with engineered structures in the nano-meter range are desirable.<br />
The properties of nucleic acids makes them a perfect material for this purpose: Nucleic acids have a regular<br />
structure with a 0.34 nm period, through its sequence it is addressable in a nanometer range. This bottom-up<br />
technique will facilitate to construct nanometer scale “nanoarrays”. Technically, DNA-oligomers are immobilised<br />
on multiple points in an ordered way. It is important to retain its functionality, the ability to hybridise to a<br />
complementary nucleic acid or to get recognised by binding proteins. Longer DNA fragments are stretched and<br />
immobilised by hybridisation between a given structure of oligonucleotides in the µm range. Here we present<br />
and compare different approaches to realise this oligo-nucleotide structures. Employed techniques include<br />
micro painting, photochemistry, self-organised monolayers on metal surfaces and others. Of importance is the<br />
stretching of long DNA, that we facilitate by application of AC fields. Scanning probe microscopy (AFM and<br />
SNOM) and laser scanning microscopy (LSM) are used to analyse these structures. Beside possible applications<br />
of the DNA-modified surfaces as DNA arrays with in-gene resolution, it will provide a framework for building<br />
larger rational assemblies used e.g., in nanoarrays, coupled enzyme reactions or even DNA based computing and<br />
macromolecular machines.<br />
10:30 am Thursday, February 5 Genomics – Advanced Topics Room A1<br />
Simon Roberts<br />
DOE Joint Genome Institute<br />
2800 Mitchell Drive<br />
Walnut Creek, California 94583<br />
srroberts@lbl.gov<br />
Co-Author(s)<br />
Martin Pollard<br />
Increasing Instrumentation Availability by Using Reliability Centered Maintenance (RCM)<br />
Principles and Applying Them to Production Line Instrumentation at the DOE Joint<br />
Genome Institute<br />
Reliability Centered Maintenance (RCM) is a process used to determine systematically and scientifically what<br />
must be done to ensure that physical assets (Laboratory Instruments) continue to do what their users want them<br />
to do. Its key goals are to produce sustained and substantial improvements in instrument availability, reliability<br />
and product quality with the added benefits of improved safety and environmental integrity. The DNA sequencing<br />
production line at the Joint Genome Institute (JGI) is characterized by modular machine stations with stacks<br />
of microtiter plates moving between them. The DNA sequencer run-rate determines the throughput for the<br />
production line. JGI is currently producing up to 1.8 GB of sequence per month. The production instrumentation<br />
engineering goals focus on increasing the quality and reliability at each process step and allowing for maximum<br />
operator efficiency. This presentation will show how RCM principles have been implemented giving specific<br />
examples of instrument modifications made. It will also show what preventative maintenance actions we have<br />
instigated and the effects on instrument reliability & availability. This work was performed under the auspices of<br />
the US Department of Energy’s Office of Science, Biological and Environmental Research Program and the by the<br />
University of California, Lawrence Livermore National Laboratory under Contract No. W-7405-Eng-48, Lawrence<br />
Berkeley National Laboratory under contract No. DE-AC03-76SF00098 and Los Alamos National Laboratory under<br />
contract No. W-7405-ENG-36.<br />
PODIUM ABSTRACTS
11:00 am Thursday, February 5 Genomics – Advanced Topics Room A1<br />
Sheri Olson<br />
Applied Biosystems<br />
850 Lincoln Center Drive<br />
Foster City, California 94404<br />
olsonsj@appliedbiosystems.com<br />
Co-Author(s)<br />
Lewis T. F. Wogan, Warren Tom, Charles Hsieh, Kyle Leinen<br />
Applied Biosystems<br />
106<br />
Gerri Shaw, Jim Shaw<br />
Bernard Shaw International<br />
Evaluation of High Throughput SNP Genotyping Lab Data Using FOCUS Statistical Software<br />
FOCUS statistical software (version 4.0) was used to evaluate daily processing efficiency and instrument<br />
functionality in a High Throughput Genotyping lab using the fluorogenic 5´ nuclease (TaqMan ® ) assay at Applied<br />
Biosystems. Laboratory processing data related to a specific customer project generated over thirty-five different<br />
processing days were recorded by an internally developed Laboratory Information Management System (LIMS).<br />
The LIMS captured reagent, consumable, instrument and operator information. The LIMS data was merged with<br />
corresponding data from internally developed Genotyping analysis software to create Daily Query Results files<br />
(DQRs). The DQRs were uploaded into the FOCUS software and the FOCUS Pre-Processor tool was used to<br />
isolate specific factor sets to assess processing efficiency and instrument performance. In the High Throughput<br />
Genotyping lab at Applied Biosystems, automated liquid handling instrumentation is used to prepare the<br />
Polymerase Chain Reaction (PCR) based TaqMan ® assay to evaluate Single Nucleotide Polymorphisms (SNPs) in a<br />
384-well format. Consequently, the performance of the liquid handling aut<strong>omation</strong> has a significant impact on the<br />
genotyping results and on the efficiency of the overall genotyping process. Therefore, FOCUS statistical software<br />
was used to evaluate the liquid handling performance for each reaction plate processed as part of the customer<br />
project. Additionally, acceptance specifications incorporated in the Genotyping analysis software categorized the<br />
resulting genotype data with either a “passing” or “REDO” status. The FOCUS software also evaluated the results<br />
associated with a “REDO” status to calculate daily processing efficiencies.
11:30 am Thursday, February 5 Genomics – Advanced Topics Room A1<br />
Ralf Muckenhirn<br />
Fraunhofer IPA<br />
Nobel Strasse 12<br />
Stuttgart, 70569 Germany<br />
rhm@ipa.fhg.de<br />
107<br />
Co-Author(s)<br />
Philipp Dreiss<br />
Johann Dorner<br />
Akhauri P. Kumar<br />
Future Clinical Analysis: Component Based Framework for Modular Hardware and Software<br />
Integration of Different Clinical Equipment<br />
The Component Based Framework provides the mechanism to define the existing clinical equipment in form of<br />
hardware modules, which can be a combination of different analysis equipment performing specific operations<br />
or it can be, containers which carry the incoming and outgoing samples or it can be, manufacturing islands<br />
such as clinical equipments not integrated in the current Laboratory Information Management Systems (LIMS).<br />
Thus defined hardware modules can be clustered together with central handling system (cluster) responsible<br />
for transporting the samples to the individual modules (analysis equipments) These modules can be connected/<br />
disconnected to/from the clusters dynamically, even in an ongoing analysis process. This allows the system<br />
wide scheduling to optimize and configure the analysis area based on the actual needs. In addition to this the<br />
Component Based Framework provides dedicated services such as:<br />
• centralized database to store sample information and its current position<br />
• centralized access rights management to establish equipment specific access rights configuration,<br />
• recipe management system to provide recipes on demand,<br />
• equipment management component to administrate the analysis equipment,<br />
• analysis planning unit providing connectivity to LIMS and translating incoming sample information to analysis<br />
area internal sample information,<br />
• scheduler component to interpret the internal information and to generate a schedule,<br />
• process management component to execute the analysis specific process recipes<br />
The presentation will show the hardware mapping of these modules by the software and the corresponding<br />
required software components as discussed above to show the easy and rapid deployment of such frameworks<br />
allowing a very high degree of flexibility in sense of adaptation to new analysis steps.<br />
PODIUM ABSTRACTS
3:00 pm Tuesday, February 3 Clinical – Proteomics Room C1<br />
Daniel W. Chan<br />
Johns Hopkins University<br />
600 N. Wolfe Street<br />
Baltimore, Maryland 21287<br />
dchan@jhmi.edu<br />
Clinical Proteomics 2004<br />
The future clinical diagnostics will be expected to provide more clinically useful information and cost less. Is<br />
Proteomics the answer? In United States, one in four deaths is caused by cancer. Since cancer is a proteomic<br />
disease, the study of cancer proteomics not only allow us to better understand the biology of cancer, but it<br />
also provide us the opportunities to identify the dysfunctional protein as potential target for therapy and protein<br />
biomarkers for cancer diagnostics. Since cancer is heterogeneous, it is unlikely that a single biomarker or protein<br />
could be used to achieve its full clinical potentials, such as screening, detection, prediction or monitoring of<br />
cancer therapy. Maximum clinical usefulness is likely to require a panel of biomarkers. New approaches to identify<br />
biomarkers that could be used individually or in combination for cost-effective screening of diseases are urgently<br />
needed. My presentation will focus on the use of surface-enhanced laser desorption-ionization (SELDI) protein<br />
chips coupled with time-of-flight mass spectrometry (MALDI-TOF). Because large amounts of data are often<br />
generated, the effective and appropriate use of bioinformatics tools become critical to analyze the expression data<br />
and to avoid selecting biomarkers whose performances are influenced mostly by non-disease related artifacts in<br />
the data.<br />
In summary, the combination of proteomics and bioinformatics tools could facilitate the identification of proteins of<br />
clinical interest. I will present some of our work at the Johns Hopkins Biomarker Discovery Center and the HUPO<br />
plasma proteome initiative.<br />
3:30 pm Tuesday, February 3 Clinical – Proteomics Room C1<br />
Keith Ashman<br />
MDS Sciex<br />
71 Valley Drive<br />
Concord, Ontario, L4K 4V8 Canada<br />
keith.ashman@sciex.com<br />
Combining LC and MALDI Mass Spectrometry: A Way to a Simpler Workflow?<br />
108<br />
Co-Author(s)<br />
Chris Lock<br />
It will be shown that while the benefits of LC fractionation prior to MALDI analysis for even single protein digests<br />
are evident, the real gains are made in the analysis of highly complex samples such as serum, containing dozens<br />
of peptides from multiple proteins. Signal suppression and competition during the ionisation process will severely<br />
limit the coverage obtainable if such samples undergo a single spot MALDI analysis. The benefits of separation<br />
also extend to MS/MS analysis. Sample longevity is always a consideration when many MS/MS acquisitions<br />
are required, but by spreading the peptides across many sample spots the number of obtainable MS/MS<br />
spectra before all sample is consumed increases greatly.The decoupling of the MALDI ionisation process from<br />
the TOF analyser in the o-MALDI 2 QSTAR XL mass spectrometer enables the instrument to demonstrate and<br />
maintain superb mass accuracy and sensitivity in both MS and MS/MS modes of operation. Internal calibrants<br />
are unnecessary and the sample surface morphology has no impact on instrument performance (an important<br />
consideration in LC/ MALDI where the organic composition and hence morphology of each sample spot as it dries<br />
will vary). This also allows a variety of sample preparation devices to be used. These benefits will be of signifcant<br />
advantage as mass spectrometric techniques become more widely used in the search for and use of disease<br />
markers for clinical diagnostics. Especially if the sample preparation methods are both simple and automated.
4:00 pm Tuesday, February 3 Clinical – Proteomics Room C1<br />
Larry Gold<br />
SomaLogic, Inc.<br />
1745 38th Street<br />
Boulder, Colorado 80301<br />
lgold@somalogic.com<br />
Protein Arrays: Movement Toward Clinical Value<br />
Multiplexed photoaptamer-based arrays have been developed for candidate human proteins. Photoaptamers are<br />
comprised of single-stranded nucleic acids and function as though they were antibodies, except that they are able<br />
to bind covalently to an intended protein. Photoaptamer arrays allow for the simultaneous measurement of proteins<br />
of interest in serum samples or other biological matrices. Since photoaptamers are covalently bound to their target<br />
analytes before signal detection, the arrays can be vigorously washed to remove background proteins, yielding<br />
superior signal-to-noise ratios and thus very low limits of detection. Photoaptamers recognize conformational<br />
protein epitopes and thus may be used to distinguish closely related proteins, including different me<strong>mbers</strong> of<br />
the same protein family; apo/holo forms of cofactor-dependent proteins may also be distinguished. Initial arrays<br />
target proteins involved in angiogenesis and inflammation, two biological processes that are involved in a large<br />
number of disease states. The multiplexed array measurements were used to assess differences between sera<br />
collected from normal and diseased patients. Three diseased states were examined: stomach cancer, lung cancer,<br />
and Crohn’s disease. The data revealed differential protein profiles between the various diseased and normal<br />
sample populations. Protein distribution profiles for the multiplexed array data will be presented. These results<br />
are the beginnings of disease signature work in which disease states may be diagnosed by measuring a suite of<br />
proteins in serum samples. SomaLogic photoaptamer arrays may be used for basic research, for critical protein<br />
measurements during drug development and clinical trials, or for true in vitro diagnostics.<br />
4:30 pm Tuesday, February 3 Clinical – Proteomics Room C1<br />
Larry Kricka<br />
University of Pennsylvania Medical Center<br />
3400 Spruce Street<br />
Philadelphia, Pennsylvania 19104<br />
kricka@mail.med.upenn.edu<br />
Current Status of Microdevices in the Clinical Laboratory<br />
The different types of microchips (microfluidic chips, biochips, bioelectronic chips, protein and DNA microarrays)<br />
are the subject of intense research and development activity in the analytical sciences. Microchips are produced<br />
using manufacturing techniques originally developed in the microelectronics (e.g., photolithography) and in the<br />
printing industry (e.g., ink jetting). Capillary electrophoresis microchips chips, protein and DNA microarrays, and<br />
patch-clamp microchips provide examples of early successes in the commercialization of microchip devices.<br />
These chips have demonstrated the benefits of microminiaturization of analytical techniques – namely downsizing<br />
of sample, reagents and controls, more rapid and convenient assays, and integration of all of the steps in an<br />
analytical process on a single chip (the so-called “lab-on-a-chip”). But, despite this progress, many challenges<br />
remain before there can be widespread and routine implementation of micro-analytical devices. These include<br />
surface chemistry effects in the sub-microliter confines of a microchip chamber, the interface between the
8:00 am Wednesday, February 4 Clinical – POC Room C1<br />
James Nichols<br />
Baystate Health System<br />
759 Chestnut Street<br />
Springfield, Massachusetts 01199<br />
james.nichols@bhs.org<br />
Reducing Medical Errors at the Point-of-Care<br />
Recent news has popularized the rate of medical errors, citing as many as 44,000-98,000 deaths annually. While<br />
many of these errors are medication related, the laboratory is not excluded. The analytical nature of laboratory<br />
personnel situates our career as a prime consultant on hospital quality improvement teams. This presentation will<br />
define strategies to better identify potential errors and develop systems to minimize the effects of errors. Point-ofcare<br />
testing, or laboratory testing performed at or near the site of patient care, will be utilized to exemplify quality<br />
assurance issues that have wide applicability to effective selection of tests, and the interpretation and utilization of<br />
test results in general. A recent Office of the Inspector General report indicates that as many as 50% of physician<br />
office laboratories do not currently meet minimal federal CLIA guidelines for laboratory testing. What is the benefit<br />
to the patient of obtaining a faster POCT result if the quality of the result is questionable? The role of information<br />
management will be stressed as means of not only automating quality documentation and error reduction, but<br />
in result data-mining and formation of clinical knowledge bases which can assist in the development of practice<br />
guidelines, outlining the most effective pathways to measurable patient outcomes and the integration of laboratory<br />
testing in those pathways.<br />
8:30 am Wednesday, February 4 Clinical – POC Room C1<br />
Randall Roy<br />
Affiliated Laboratory, Inc.<br />
489 State Street<br />
Bangor, Maine 04401<br />
rroy@emh.org<br />
Implementation of POC Web Connectivity<br />
For how many years have you been preaching the virtues of connectivity for all Point of Care testing? How many<br />
articles have you read and how many seminars have you attended that have vehemently supported the goals of<br />
connectivity? How many times have you, as a POCT coordinator pushed your vendors of POCT instruments to<br />
provide a connectivity element with every instrument? Anyone who has been on the laboratory front for some time<br />
knows that this has been a goal for almost 15 years. We have experienced the glacial movement of the industry<br />
and have been frustrated by the lack of response. The pioneers and eternal optimist with a dominant technology<br />
gene in our ranks decided that the industry would not move forward until we provided specific information about<br />
what we wanted. So a committee was formed to accomplish this task. The task was completed quickly. The<br />
turnaround time was enough to impress any Emergency Department physician. The National Committee for Clinical<br />
Laboratory Standards published the standards. We are still waiting, but we are getting close.The most promising<br />
and least expensive technology is web-based connectivity. The State of Maine promotes itself with the saying,<br />
“Maine, the way life should be.” This is the message and experience I have had in connecting seven hospitals, two<br />
dialysis centers and one out reach center spread across 200 miles of Maine. “Web-based connectivity, the way<br />
POCT should be.”<br />
110
9:00 am Wednesday, February 4 Clinical – POC Room C1<br />
Louis Dunka<br />
LifeScan<br />
1000 Gibraltar Drive<br />
Milpitas, California 95035-6314<br />
ldunka@lfsus.jnj.com<br />
Clinical Outcomes From Point of Care Connectivity<br />
Several studies have been reported which demonstrate the benefits of Point of Care Connectivity in the areas of<br />
workflow, regulatory compliance and financail outcomes. Efforts over the past few years have resulted in standards<br />
for interfaces for Point of Care devices. At the same time, multi-analyte vendor nuetral data management systems<br />
have become available. The confluence of these events has resulted in a an opportunity to look at data at both<br />
the data manager level and the Lab Information Sytem level to understand how better patient outcomes can be<br />
achieved. Examples of these efforts to turn data into actionable information include examination of insulin dosing<br />
practices on patients with diabetes at two hospitals, using data from the LIS, resulting in a significant simplifaction<br />
of standard practices, resulting in better patient care. In another example, data was extracted from the LIS to<br />
compare lab glucose results to results obtained on patients home blood glucose monitors within a 10 minute<br />
period in an outpatient clinic. This allowed an assessment of the comparability of the results and a retraining of the<br />
patient or replacement of the blood glucose monitor, where necessary. A third study looked at when glucose tests<br />
had been performed by the lab and by fingerstick at the patient’s bedside within 15 minutes of each other. Analysis<br />
of the data allowed a change in standard practice which allowed the hospital to minimize the number of times a<br />
patient is tested.<br />
9:30 am Wednesday, February 4 Clinical – POC Room C1<br />
Frederick Kiechle<br />
William Beaumont Hospital<br />
3601 W. 13 Mile<br />
Royal Oak, Michigan 48073<br />
fkiechle@beaumont.edu<br />
Compliance, Connectivity, and POCT<br />
111<br />
Co-Author(s)<br />
Leana Salka<br />
Recently we implemented POCT connectivity using the Remote Automated Laboratory System (RALS Plus),<br />
which linked 61 glucose testing sites, 120 glucose meters, and 3,500 operators to a data management system. To<br />
determine the impact of connectivity on the glucose POCT program, software features such as operator lockout,<br />
quality control lockout, and the labor cost for the POCT coordinator were evaluated for a period of 3 months<br />
before and after its implementation. Operator and quality control lockout decreased nursing labor costs by $45.44<br />
every 3 months and POCT coordinator labor costs by $760.89 every 3 months. RALS Plus software reduced<br />
the labor cost of managing the Inform glucose meter database. The POCT coordinator’s labor cost to download<br />
updated data manually from the laptop to the Accu-Data GTS was reduced by $1,043.79. RALS Plus was<br />
interfaced bidirectionally with the hospital information system. When the Inform database is docked in the base<br />
unit, the results download automatically using an infrared sensor to the hospital information system, thus reducing<br />
clerical errors associated with manual result entry and labor costs to nursing by $32,627.90 every 3 months and<br />
to the POCT coordinator by $468.24 every 3 months. In conclusion, POC connectivity reduces error, increases<br />
program compliance, and decrease POCT coordinator and nursing costs. POC connectivity resulted in a total<br />
annual cost savings of $119,095.00<br />
PODIUM ABSTRACTS
10:30 am Wednesday, February 4 Clinical – Molecular Diagnostic Room C1<br />
Guido Baechler<br />
Roche Molecular Systems<br />
4300 Hacienda Drive<br />
Pleasanton, California 94566<br />
guido.baechler@roche.com<br />
NAT Aut<strong>omation</strong> – Enabling Transformation of Molecular Technology From Research to IVD<br />
Today, nucleic acid testing (NAT) is mainly performed in highly technical labs. Less than 6.5% of the hospital based<br />
clinical diagnostic labs worldwide perform NAT. Some of the key factors that prevent a wider utilization of NAT<br />
technology include the highly technical skill level required, the need for additional, dedicated laboratory space and<br />
the need to acquire additional equipment. Appropriate aut<strong>omation</strong> will serve to overcome the technical hurdles<br />
while helping to solve the economic hurdles for wider utilization of NAT in the marketplace.“NAT Aut<strong>omation</strong> –<br />
Enabling transformation of molecular technology from Research to IVD” will introduce you to the current market<br />
trends in NAT testing, outline key economic drivers and the opportunity for aut<strong>omation</strong>. The presentation will focus<br />
on the laboratory requirements needed to perform NAT testing and how Roche Diagnostics has overcome the<br />
challenges of aut<strong>omation</strong> to enable a wider utilization of the NAT technology.<br />
11:00 am Wednesday, February 4 Clinical – Molecular Diagnostic Room C1<br />
Hasnah Hamdan<br />
Quest Diagnostics Nichols Institute<br />
33608 Ortega Highway<br />
San Juan Capistrano, California 92690<br />
hamdanh@questdiagnostics.com<br />
High Throughput Molecular Infectious Diseases Tests in the Routine Clinical Laboratory<br />
Environment<br />
Molecular tests for infectious diseases require extraction and amplification of nucleic acid and detection of<br />
amplified products. Numerous factors must be considered when automating nucleic acid tests (NATs) for the<br />
clinical laboratory: types of infectious agents, types of specimens, handling and exposure to infectious material,<br />
contamination control, and use of different amplification methods. Currently, only a few NATs have been FDA<br />
cleared for infectious diseases. Moreover, these tests are either not automated or only semi-automated. Most<br />
existing aut<strong>omation</strong> systems for infectious disease NATs are for the amplification and detection processes; limited<br />
aut<strong>omation</strong> is available for nucleic acid extraction, which is the most labor-intensive and challenging of all the<br />
processes in NATs. Some solutions for automating NATs for the infectious disease laboratory will be presented.<br />
112
11:30 am Wednesday, February 4 Clinical – Molecular Diagnostic Room C1<br />
Jeffrey Allen<br />
Gen-Probe, Inc.<br />
10210 Genetic Center Drive<br />
San Diego, California 92121<br />
jeffa@gen-probe.com<br />
Process Control With Automated NAAT Systems<br />
Molecular diagnostic assays entering the clinical laboratory are rapidly increasing in number. However clinical<br />
laboratories are experiencing an ever increasing shortage of highly skilled personnel necessary to run these<br />
molecular methods which require significant “hands-on” technique. Aut<strong>omation</strong> of nucleic acid amplification testing<br />
(NAAT) offers a solution that simultaneously addresses both increasing testing volume and acute labor shortage.<br />
Yet aut<strong>omation</strong> alone is insufficient to address the concerns facing the clinical lab or blood testing facility. For<br />
many laboratories, the quality of the results, i.e., “process control” particularly for NAAT assays, has taken on<br />
greater importance than overall sample through-put. New systems for molecular testing must not only save labor,<br />
but have design features incorporated which provide system performance “checks” throughout the process. While<br />
many clinical chemistry analyzers can determine if sufficient sample volume is present in the primary tube, next<br />
generation platforms must confirm sufficient sample was transferred to the reaction vessel. Molecular techniques<br />
compound the technical challenges in implementing process control, to help ensure quality results, due to the<br />
extreme sensitivities inherent with NAAT and the resulting potential for contamination. Gen-Probe, Incorporated<br />
is currently developing the TIGRIS DTS system which is the first system that not only automates NAAT<br />
procedures, but also provides extensive process control design features (such as: “Reagent Dispense Verification”),<br />
that confirm the assay result integrity. Both hardware and software design features will be reviewed to illustrate<br />
how process control can be implemented with systems capable of detecting less than 100 copies of nucleic acid<br />
target sequence in a clinical sample.<br />
12:00 pm Wednesday, February 4 Clinical – Molecular Diagnostic Room C1<br />
Zhili Lin<br />
Pediatrix Screening, Inc.<br />
90 Emerson Lane, Suite 1403<br />
Bridgeville, Pennsylvania 15017<br />
zlin@neogenscreening.com<br />
113<br />
Co-Author(s)<br />
Joseph G. Suzow<br />
Jamie M. Fontaine<br />
Edwin W. Naylor<br />
Primary DNA-based Newborn Screening of Sickle Cell Disease and Hemoglobinopathy<br />
For a population-based newborn screening program, challenges exist in using technological advances to improve<br />
the quality and efficiency of the existing screening program and to develop new diagnostic capabilities. A newly<br />
developed genotyping method for screening of common mutations within the beta-globin gene is described<br />
here. This genotyping system consists of three major components: an aut<strong>omation</strong> system for high throughput<br />
DNA extraction and PCR setup, a conventional thermal cycler, and a LightTyper instrument for post-PCR melting<br />
temperature analysis. Briefly, genomic DNA is extracted from dried blood on a filter paper using the common<br />
chemicals methanol and Tris buffer. Genetic fragments of interest are amplified by asymmetric PCR. Fluorescent<br />
labeled probes are added during PCR setup, which eliminates the need for any post-PCR sample handling<br />
process. Melting temperature analysis is achieved through fluorescent resonance energy transfer (FRET) reaction<br />
using the LightTyper instrument. The assay is designed to simultaneously detect three common beta-globin<br />
mutations, S(A173T), C(G172A), and E(G232A), and can identify any of the eight possible genotypes in a single<br />
reaction: AA, AE, EE, AS, SC, SS, AC, and CC (A represents wild type allele). The method was validated with a<br />
large number of samples in both a retrospective and parallel study. Results were compared to those obtained by<br />
isoelectric focusing electrophoresis. The accuracy of this genotyping method is greater than 99%.<br />
PODIUM ABSTRACTS
3:30 pm Wednesday, February 4 Clinical – Informatics I Room C1<br />
John Saulenas<br />
MEDITECH<br />
One MEDITECH Circle<br />
Westwood, Massachusetts 02090<br />
jsaulenas@meditech.com<br />
Autoverification: Paving the Path to Efficiency and Quality<br />
Clinical laboratories face an ever-increasing workload, a decreasing staff of skilled technologists, and the neverending<br />
requirement to “do more with less.” As a result, many laboratories are looking for ways to stream-line<br />
their processes. Automated solutions for the pre-analytical and analytical processes of the laboratory have been<br />
implemented ; Autoverification, also referred to as autorelease or autovalidation, is a post-analytical process that<br />
has been available for nearly a decade but has not been adopted in many laboratories. Autoverification uses<br />
computerized rules to review laboratory data, release results to the healthcare information system and determine<br />
which results need further review by a technologist. If implemented correctly, autoverification can significantly<br />
improve a laboratory’s quality and efficiency. Attendees will come away with a better understanding of how<br />
autoverification can be used to improve patient safety, enhance the working environment for laboratory staff and<br />
facilitate better patient care.<br />
4:00 pm Wednesday, February 4 Clinical – Informatics I Room C1<br />
Leo Serrano<br />
West Tennessee Healthcare<br />
708 West Forest Avenue<br />
Jackson, Tennessee 38301<br />
leo.serrano@wth.org<br />
Autoverification of Laboratory Results – A Real Life Perspective<br />
The presentation will discuss the processes, instrumentation issues, requirements and benefits of<br />
autoverification of lab results in a real clinical setting. Issues discussed will include processes and metrics<br />
before the implementation of autoverification, the stepwise implementation of autoverification, the selection of<br />
instrumentation, including the criteria for autoverification in the various disciplines of the lab. Metrics will be<br />
presented showing the improvement in performance and productivity<br />
114
4:30 pm Wednesday, February 4 Clinical – Informatics I Room C1<br />
David Velasquez<br />
Mills-Peninsula Health Services<br />
1783 El Camino Real<br />
Burlingame, California 94010<br />
velasqd@sutterhealth.org<br />
Autoverification and Regulatory Issues: The Rules and Regulations According to CLIA, CAP,<br />
and the California Department of Health Services<br />
Autoverification is rapidly becoming the industry standard for post-analytic processing and quality enhancements.<br />
In the highly regulated world of Clinical Laboratories it is important for medical and technical leadership to<br />
understand the various regulations and how they apply to autoverification. Because CLIA regulations for<br />
autoverification are not clearly delineated and fall loosely under the Medical Director’s discretion, caution must<br />
be exercised when implementing autoverification. The College of American Pathologists has developed very<br />
clear standards for the implementation and oversight of autoverification systems. The CAP has deemed authority<br />
for CLIA regulations and therefore a careful overlap in the two sets of rules can assist a lab in developing and<br />
implementing autoverification systems with regulatory assurance. Title 17 of the California Code of Regulations<br />
contains language that is prohibiting the implementation of autoverification in the State of California. We will review<br />
the history of these regulations and look into the future of a changing California Laboratory industry.<br />
5:00 pm Wednesday, February 4 Clinical – Informatics I Room C1<br />
William Neeley<br />
Detroit Medical Center<br />
4201 St. Antoine 3E-1, UHC<br />
Detroit, Michigan 48201<br />
wneeley@dmc.org<br />
Post-Analytic Intelligent Systems vs. Autoverification: The Future<br />
Over the last decade, autoverification has proven itself to enhance the timely delivery of results, save labor and<br />
improve overall laboratory efficiency. Although autoverification is now being used by large nu<strong>mbers</strong> of laboratories,<br />
its full potential has not been fully realized because it fails to meet with the expectations of the medical leadership<br />
of most laboratories. In most cases, the algorithms are pedestrian and relegated to simple testing for high and low<br />
limits per individual test. Similarly, delta testing is also limited to evaluation by individual test. The next generation<br />
of software will be post-analytic intelligent systems that are able to handle more complex algorithms allowing for<br />
evaluations of the relationships between multiple tests as well as taking into account the prior results of multiple<br />
tests. Delta testing will be included in the complex algorithms. As these algorithms develop, it is very important<br />
that they also have the ability to be customized to meet the needs of their laboratories based on their physician<br />
requirements and their patient mix. Future enhancements will include the ability to provide custom solutions – by<br />
physician – based on the physician’s particular needs and interests. Communication with physicians will be done<br />
automatically via email, pager, hand-held device or other means of choice. The notification criteria will be tailored<br />
to meet an individual physician’s needs, optimal times and desired frequency. The next generations of post-analytic<br />
intelligent software will provide significant improvements and benefits for our patients, physicians and laboratories<br />
over the current versions of autoverification.<br />
115<br />
PODIUM ABSTRACTS
8:00 am Thursday, February 5 Clinical – Informatics 2 Room C1<br />
Charles Hawker<br />
ARUP Laboratories, Inc.<br />
500 Chipeta Way<br />
Salt Lake City, Utah 84108<br />
hawkercd@aruplab.com<br />
The Role of Information Systems in Aut<strong>omation</strong> in a Large Reference Laboratory<br />
ARUP, a large national esoteric reference laboratory, averages 20,000 specimens per day for nearly 2500 different<br />
tests. Since 1998, we have employed a 2000 tube/hour MDS AutoLab automated transport/sorting system and<br />
an internally developed information system called Expert Specimen Processing (ESP). Since implementation 5<br />
years ago, and despite more than doubling in size, we have improved productivity by 23%, saving an estimated 62<br />
full time employee equivalents. We have reduced median turn-around time (TAT) by 15%, 95th percentile TAT by<br />
18%, and lost specimens by 68%. To meet further significant growth, we expanded the AutoLab system to 4000<br />
tubes/hour. We implemented a Motoman robotic sorter for transferring specimens from the AutoLab system to<br />
stainless steel trays for archival storage, an SK Daifuku automated storage and retrieval system (AS/RS) in a -20 C<br />
freezer that holds >5200 such trays, and a Motoman robotic archive picker that picks requested tubes from trays<br />
and delivers them in small plastic racks to users outside the freezer. The combined retrieval speed of the AS/RS<br />
and the robotic picker is 1.25 minutes for any single tube among potentially 2.35 million stored tubes. Customized<br />
information systems were provided by AutoLab, SK Daifuku, and Motoman for their aut<strong>omation</strong> systems. These<br />
are interfaced to ESP, which is the primary controlling system. ESP is also interfaced to our LIS which is Pathnet<br />
3.06 (Cerner). Many modifications were made to these software systems to improve their usefulness and to<br />
increase the productivity of our employees.<br />
8:30 am Thursday, February 5 Clinical – Informatics 2 Room C1<br />
Marcy Anderson<br />
Medical Aut<strong>omation</strong> Systems<br />
7880 Manor Drive<br />
Harrisburg, Pennsylvania 17112<br />
manderson@rals.com<br />
Clinical Informatics – “Middleware”<br />
116<br />
Co-Author(s)<br />
Charles Hawker, ARUP Laboratories, Inc.<br />
Jay B. Jones, Geisinger Health System<br />
Hunter Bagwell, Roche Diagnostics<br />
Point of Care Testing (POCT) Connectivity and Data Management is a growing field. Glucose and other POCT<br />
devices embrace this method of capturing data as one of the product’s standard features. Results obtained<br />
from these devices can be managed in several ways. Patient results need to ultimately be placed on the patient<br />
permanent medical record. Quality Control information needs to be effectively managed to ensure accuracy<br />
and regulatory compliance. To efficiently run a comprehensive program, it is important to know what features of<br />
a connectivity solution will meet the needs of your institution. In addition, the hurdles of implementing several<br />
POCT devices into one’s POCT program need to be defined. These can include (but are not limited to) regulatory<br />
compliance, managing the use of correct patient identification, and managing the use of correct operator ids. With<br />
a data management/connectivity solution these obstacles can be minimized. This discussion will provide insight on<br />
choosing a connectivity package, implementing it effectively, and growing with it to meet the challenges of running<br />
a successful POCT program.
9:00 am Thursday, February 5 Clinical – Informatics 2 Room C1<br />
Hunter Bagwell<br />
Roche Diagnostics<br />
9115 Hague Road<br />
P.O. Box 50457<br />
Indianapolis, Indiana 46250-0457<br />
hunter.bagwell@roche.com<br />
Middleware Solutions – Optimizing People, Processes, and Platforms<br />
Today’s laboratory faces more challenges and tighter financial scrutiny than ever before. While workloads are<br />
increasing and reimbursements are declining, labs are expected to find qualified personnel from a shrinking labor<br />
pool while trying to grow volumes with limited resources. As an answer to these issues, Roche Diagnostics has<br />
developed Middleware Solutions. Middleware Solutions powered by Data Innovations connects your existing LIS<br />
and instrumentation allowing you to automate information and fill the gaps in your current information system. By<br />
providing advanced features such as auto verification you can eliminate up to 80% of the labor associated with<br />
results review and allowing better utilization of your scarce resources. The advanced archiving package allows you<br />
to find samples in less than a minute, saving you over 60% of the labor associated with retrieving samples and<br />
removing mundane tasks. At the heart of the system is rules based decision processing. Rules are constructed<br />
through an easy-to-use graphical user interface that allows you to build compound IF / THEN statements to<br />
customize your information workflow. This allows you to incorporate your client’s testing criteria automatically<br />
providing tailored solutions for your customers. Bottom line, Middleware Solutions from Roche Diagnostics allows<br />
you to quickly optimize the performance of your people, processes and platforms without added complexity.<br />
9:30 am Thursday, February 5 Clinical – Informatics 2 Room C1<br />
Jay Burton Jones<br />
Geisinger Health System<br />
Geisinger Medical Center 01-31<br />
Danville, Pennsylvania 17821-0131<br />
JBuJones@geisinger.edu<br />
Data Mining From the LIS With Simple PC Tools<br />
A practical, accessible, and user friendly “data mining” toolset is described that extracts 15 designated data<br />
fields from the Laboratory Information System (LIS) via a Crystal ad hoc report writer and exports comma<br />
delimited (.csv) files to a network secure PC. These extracted clinical lab results are “data mined” with MS Access<br />
and MS Excel and subsequently graphed in standard MS Excel format. Histogrammed clinical lab data assists<br />
in verifying reference ranges (age/sex stratified), judging utilization statistics, performing moving average quality<br />
control studies, and comparing analytical methods on actual patient data. “Data mined” population data from<br />
a large rural HMO will be presented for thyroid, lipid, diabetes, and point-of-care testing (POCT). LIS results<br />
(typically N=100 – 300K) are put through the Crystal extraction process in 1 – 3 hours and “data mined” at the PC<br />
workstation in 4 – 8 hours. The Crystal extraction process is automated and placed under user control. Since the<br />
LIS is the “source of truth” data warehouse for archived POCT as well as central laboratory results, “data mining”<br />
may help verify their relative accuracy. POCT glucose tests (y; N= 410K) are compared to central lab glucose<br />
tests (x; N=1.1M), sorted for those tested on the same patient within 10 minutes (N= 14.3K), and correlated in<br />
MS Excel.(y = 0.99x + 5). These data suggest over-utilized glucose “double testing” and that real-time POCT<br />
may provide more useful real time information. These readily available “data mining” tools (essentially “back-end<br />
middleware”) may form the basis for peer comparison of LIS data in the future.<br />
117<br />
PODIUM ABSTRACTS
10:30 am Thursday, February 5 Clinical – Arrays Room C1<br />
John Walker<br />
GNF<br />
10675 John Jay Hopkins Drive<br />
San Diego, California 92121<br />
walker@gnf.org<br />
Using Reference Gene Expression Datasets to Make Sense of Your Array Data<br />
118<br />
Co-Author(s)<br />
John Hogenesch, Andrew Su,<br />
Sergei Batalov, Michael Cooke,<br />
Jie Zhang<br />
Gene function is the major focus of Genomics research today. Where and under which conditions a gene is<br />
expressed can give information on its function. We have made a major effort in providing the public with gene<br />
expression data from normal human and rodent tissue. I will discuss how we and others have used this data to<br />
find genes responsible for human disease and mouse phenotypes. Construction and future potential uses of this<br />
data, as well as upcoming public data releases, will be discussed.<br />
11:00 am Thursday, February 5 Clinical – Arrays Room C1<br />
John Palma<br />
Affymetrix, Inc.<br />
3380 Central Expressway<br />
Santa Clara, California 95051<br />
john_palma@affymetrix.com<br />
Molecular Tools Designed for the Clinic<br />
The completion of the draft sequence of the human genome, improvements in computing power and rapid<br />
advancements in microarray technology have accelerated the use of high density oligonucleotide microarrays.<br />
Researchers are maximizing the amount of information they can obtain from precious patient samples by carrying<br />
out genome wide analyses in a single experiment. In Affymetrix’ 12 years pioneering the field, it has driven the<br />
technology forward with marked improvements in reproducibility, sensitivity and information content per array.<br />
As a testament to the acceptance and versatility of the technology, over 1400 research articles have been<br />
published based on GeneChip ® array data, with over 600 in 2002. Consistent with the single platform concept,<br />
the platform can now be used for a more biologically comprehensive approach to dissecting complex disease.<br />
Arrays are available for RNA and DNA analysis, with DNA analysis applications for both SNP genotyping and<br />
custom re-sequencing efforts. In order to move arrays into the clinical arena, a number of challenges have been<br />
identified. These include, scalability for high throughput applications, establishment of standards and guidelines,<br />
recommendations from professional societies, use in large clinical studies and trials, support from advocacy<br />
groups, competitive pricing and reimbursement. Microarrays, in conjunction with patient clinical parameters<br />
and sophisticated algorithms, present the opportunity to achieve an integrated treatment approach, taking<br />
advantage of all of the clues DNA, RNA and clinical information can provide. Information regarding advances in our<br />
technology, aut<strong>omation</strong> for high throughput array handling and efforts towards the development of guidelines and<br />
standards will be presented.
11:30 am Thursday, February 5 Clinical – Arrays Room C1<br />
Richard Hockett<br />
Eli Lilly and Company<br />
Lilly Corporate Center<br />
Indianapolis, Indiana 46285<br />
hockett@lilly.com<br />
Clinical Validation of the Affymetrix Microarray and the Challenges Faced<br />
119<br />
Co-Author(s)<br />
Carmen Dumaual, Crystal Dotson,<br />
Sandra Kirkwood, Mark Farmen<br />
The use of microarray technology has resulted in a huge number of expression studies on human tissue.<br />
Identification of genes which better classify tumors, or even predict the likely response to therapy has been touted<br />
as a means to improve clinical outcome. Before microarrays can become common clinical tools, this technology<br />
must undergo a thorough validation and ultimately FDA review. We have begun to validate microarrays for use in<br />
clinical drug development and will discuss the validation plan, review validation data, and describe the numerous<br />
challenges faced during this exercise.<br />
1:30 pm Thursday, February 5 Clinical – Pharmacogenomics Room C1<br />
Audrey Papp<br />
Ohio State University<br />
5160 Graves Hall<br />
333 W. 10th. Avenue<br />
Columbus, Ohio 43210<br />
papp.2@osu.edu<br />
Potential Role of Pharmacogenomics in Reducing Risk of Adverse Reactions and<br />
Optimizing Therapy<br />
Co-Author(s)<br />
Wolfgang Sadée<br />
Numerous genes play a role in determining drug response in individual patients. Genetic variants may either affect<br />
drug efficacy or adverse effects. Adverse affects are often associated with polymorphisms in genes encoding<br />
metabolizing enzymes and transporters, or less frequently with drug receptors. On the other hand, drug efficacy<br />
may depend upon genes that also function as susceptibility factors in disease. We focus on drug efficacy<br />
in complex disorders, including coronary artery disease, CNS disorders, and cancer, each involving multiple<br />
candidate genes. As a general approach we analyze genetic variations in multiple candidate genes in clinical<br />
association studies. Multiple polymorphisms (mostly single nucleotide polymorphisms) per gene are measured<br />
to determine the main haplotypes in a population, followed by functional analysis of mRNA processing, and<br />
protein analysis as feasible. Haplotypes are either inferred by statistical methods, or are measured experimentally.<br />
This approach requires high throughput genotyping of numerous polymorphisms in large patient and control<br />
populations. In addition, quantitative analysis of alleles in DNA and mRNA is necessary. We present methods<br />
capable of addressing these complex problems, and show select results on association of candidate gene<br />
haplotypes with clinical phenotypes.<br />
PODIUM ABSTRACTS
2:00 pm Thursday, February 5 Clinical – Pharmacogenomics Room C1<br />
Carl Yamashiro<br />
Amersham Biosciences<br />
3200 W. Germann Road<br />
Chandler, Arizona 85248<br />
carl.yamashiro@amersham.com<br />
120<br />
Co-Author(s)<br />
Buena Chui, M. Roxane Bonner, Michael Gaskin,<br />
Penny Gwynne, Jeffrey Winick, Arkadiy Silbergleit<br />
Amersham Biosciences<br />
Annalee Ledesma<br />
University of California, San Diego<br />
Population Studies Using Enhanced Version of the CodeLink P450 SNP Bioarrays Yield<br />
New and Novel Genotype and Haplotype Frequency Data<br />
A significant part of variation in drug efficacy and safety is of a hereditary nature, and for many instances can<br />
be traced down to polymorphisms in genes involved in drug metabolism. Establishing correlations between<br />
drug metabolizing phenotypes and P450 genotypes and haplotypes will facilitate the drug discovery process,<br />
make clinical trials safer, and provide the next step to personalized medicine. CodeLink SNP bioarrays from<br />
Amersham Biosciences are robust tools for broad-based single nucleotide polymorphism (SNP) genotyping, which<br />
can be used in linkage mapping or candidate gene profiling. The assay is simple, straightforward, and highly<br />
efficient assay with high throughput capabilities. For SNP detection, CodeLink platform uses enzymatic allele<br />
specific extension (ASE) of oligonucleotide probes. Templates for ASE are generated by highly specific long-range<br />
multiplex PCR, despite high DNA homology within the P450 gene subfamilies, followed by amplicon purification<br />
and fragmentation. The genotyping assay has been streamlined and simplified for facile handling and more rapid<br />
time to results. CodeLink SNP P450 bioarrays allow for genotyping of 110 SNPs in nine genes: CYP1A1, CYP1A2,<br />
CYP1B1, CYP2C19, CYP2C9, CYP2D6, CYP2E, CYP3A4, and CYP3A5. We sequenced 46 samples from three<br />
major populations at all of these loci to calculate assay accuracy. From Coriell Human Diversity panels, 230<br />
samples were genotyped and population-specific allele and inferred haplotype frequencies have been determined.<br />
There are several interesting haplotypes within a few of the P450 genes for certain populations that have potential<br />
clinical ramifications with respect to pharmacogenetic outcomes.
2:30 pm Thursday, February 5 Clinical – Pharmacogenomics Room C1<br />
Stan Lilleberg<br />
Transgenomic, Inc.<br />
11 Firstfield Road, Suite E<br />
Gaithersburg, Maryland 20878<br />
slilleberg@transgenomic.com<br />
In-depth Genetic Variation Screening Using DHPLC: A Valuble Component of the Drug<br />
Development Process<br />
The genomics revolution has forever altered the landscape of pharmaceutical research and development. In<br />
addition to the discovery of new potential drug targets, the number of mutations and polymorphisms identified<br />
within individual genes is escalating. Since the biological impact of each individual genetic variation depends<br />
on its location and specific sequence alteration, there will be a constant requirement for sensitive and accurate<br />
methods to scan genes of interest for new, and often low-level, genetic variation. Denaturing high performance<br />
liquid chromatography (DHPLC) is a new technology used in the discovery of genetic variations in the form of<br />
mutations that include single base substitutions or single nucleotide polymorphisms (SNPs), as well as small<br />
deletions or insertions. These genetic variations can be routinely detected by DHPLC gene scanning at the germline<br />
and somatic levels. Epigenetic alterations such as changes in DNA methylation status at defined loci can also<br />
be assessed using DHPLC-based methodology. The biological impact of these genetic variations depends on the<br />
location and identity of the DNA sequence alteration. The discovery of functionally relevant genetic variations can<br />
be exploited throughout the drug discovery and development process. Examples of the application of DHPLC for<br />
sequence variant detection will be presented and discussed, with an emphasis on target validation by candidate<br />
gene scanning, mutation detection in disease pathway genes, and the discovery of therapeutically significant<br />
genetic variants associated with drug metabolism and resistance. In-depth genetic variation screening using<br />
DHPLC technology has accelerated the discovery of novel variants in a multitude of genes, contributing to the<br />
understanding of disease pathogenesis and future directions of drug development.<br />
3:00 pm Thursday, February 5 Clinical – Pharmacogenomics Room C1<br />
Elvan Laleli-Sahin<br />
Medical College of Wisconsin-Milwaukee<br />
Pathology Department<br />
8701 Watertown Plank Road<br />
Milwaukee, Wisconsin 53226<br />
elvan@mcw.edu<br />
121<br />
Co-Author(s)<br />
Paul Jannetto,<br />
Steven H. Wong<br />
Utilization of Pharmacogenomics in Patient Care for Pain Management Clinics and Poison<br />
Control Centers<br />
One direct application of the genetic information generated with in the last decade has been pharmacogenetics;<br />
science of explaining genetic based pharmacokinetic and pharmocodynamic variability among individuals. Use<br />
of pharmacogenetics for evaluating why certain therapies fail will be a direct and rapid clinical application. The<br />
enzyme family (cytochrome P450 – CYP) responsible for the first phase metabolism of a foreign compound, shows<br />
polymorphisms that correlate with an individual’s phenotype in response to a given drug. The phenotype can<br />
vary from no response to therapy to severe adverse drug reactions and even death due to over dose. CYP 2D6,<br />
is the enzyme responsible for metabolism of almost one quarter of prescription drugs, including analgesics. Pain<br />
management of chronic pain patients varies due to the subjective nature of pain along with the genetic variability of<br />
drug metabolizing enzymes, especially CYP 2D6. Single nucleotide polymorphisms (SNP’s) and deletion mutations<br />
within this gene have been shown to correspond with poor metabolizer phenotype. Pharmacogenetics possesses<br />
the potential to aid in efficient therapy for chronic pain patients that are proven to be difficult to manage cases. Our<br />
ongoing studies have identified 25% prevalence for intermediate metabolizers. In addition there is good correlation<br />
of genotype and response to therapy. Extensive metabolizer individuals showed no adverse reactions while an<br />
intermediate metabolizer, with normal kidney and liver functions, showed adverse side-effects to tramadol therapy.<br />
PODIUM ABSTRACTS
3:00 pm Tuesday, February 3 Emerging Technologies – Engineering Applications Room A3<br />
Reinhold Schäfer<br />
Fachhochschule Wiesbaden<br />
Kurt-Schumacher-Ring 18<br />
Wiesbaden, D-65197 Germany<br />
schaefer@informatik.fh-wiesbaden.de<br />
Dynamic Scheduling in the Laboratory – Problems and Solutions<br />
Analytical laboratories perform manifold analyses in parallel. Different analytical procedures have to be optimized<br />
towards throughput or other parameters. Primarily workflows with well-defined functionality have to be formalized<br />
in order to be computable. They describe the testing including processing the sample on various instruments,<br />
sensor control, data acquisition, result calculations and storage etc. with all timing and conditional constraints.<br />
A working plan for different samples is calculated consisting of all activities performed on different resources.<br />
This presentation discusses the need for different workflow elements, their interaction and effects of dynamic<br />
execution. In addition hidden transport implications are discussed as well as a scenario of dynamic recovery and<br />
re-scheduling in case of errors.<br />
3:30 pm Tuesday, February 3 Emerging Technologies – Engineering Applications Room A3<br />
Mathew Hahn<br />
Scitegic<br />
9665 Chesapeake Drive, Suite 401<br />
San Diego, California 92123<br />
mhahn@scitegic.com<br />
The Data Pipelining Approach to Informatics Aut<strong>omation</strong> and Integration<br />
The processing and interpretation of large volumes of automated laboratory data is burdened with numerous<br />
challenges. The Data Pipelining approach provides a software computing environment powerful enough to keep<br />
pace with data generation, yet flexible enough to adapt quickly and easily to changing processes. By streaming<br />
data between any of hundreds of available modular data processing steps, virtually unlimited functionality can<br />
be assembled. Further, with open standards for integrating 3rd party software as re-useable modular steps in the<br />
system, a Data Pipelining system can include existing resources as well as adapt to future needs as they arise. We<br />
will discuss the approach as well as the technologies employed to permit the easy integration of disparate data<br />
and external applications.<br />
122
4:00 pm Tuesday, February 3 Emerging Technologies – Engineering Applications Room A3<br />
David Dorsett<br />
Symyx Technologies, Inc.<br />
3100 Central Expressway<br />
Santa Clara, California 95051<br />
ddorsett@symyx.com<br />
Comprehensive Materials Informatics in Chemical and Pharmaceutical Research<br />
Complete integration of data collected across multiple synthetic steps and from large nu<strong>mbers</strong> of property<br />
screens is a critical aspect in enabling data-driven materials research. This presentation will outline software<br />
requirements for experimental design and instrument aut<strong>omation</strong> for high throughput systems, and provide a<br />
live demonstration of such software. Additionally, software requirements for capturing and integrating data from<br />
conventional bench and large-scale experimentation will be discussed and some current systems demonstrated.<br />
Finally, comprehensive querying and reporting systems that provide simultaneous access to high throughput and<br />
conventional research data will be presented.<br />
4:30 pm Tuesday, February 3 Emerging Technologies – Engineering Applications Room A3<br />
Robert L. Stevenson<br />
IO Informatics, Inc.<br />
2000 Powell St. #520<br />
Emeryville, California 94608<br />
rlsteven@comcast.net<br />
Rapid Evaluation, Normalization and Global Search Program for 2D Electrophoresis<br />
123<br />
Co-Author(s)<br />
Erich A. Gombocz<br />
Robert A. Stanley<br />
Traditional approaches to searching a library of large databases quickly encounter severe problems due to large<br />
size and different organization of the data, including the wraper. Brute force solutions often include clusters of<br />
more than 100 processors. IO informatics has developed a new paradigm in data searching that uses objects<br />
combined with vectorization to organize and search data files in 1% or less of the time required by traditional<br />
methods. Now it is possible to perform complex searches with a single PC. The first product is a system for 2D<br />
electropherograms that combines analysis, data mining, tracking and storage in a secure regulatory compliant<br />
manner. One can compare gels of different size and methods. Spot detection, land marking and 3D deconvolution<br />
are easy and reliable. The underlying platform provides any-to-any connectivity to facilitate searching for data from<br />
any accessible data source, including structural, functional and bioassay. The new paradigm is being expanded to<br />
other applications.<br />
PODIUM ABSTRACTS
8:00 am Wednesday, February 4 Emerging Technologies – Cell Based Screening Room A3<br />
Evan Cromwell<br />
Blueshift Biotechnologies, Inc.<br />
238 E. Caribbean Drive<br />
Sunnyvale, California 94089<br />
ecromwell@blueshiftbiotech.com<br />
Novel Cellular Analysis Platform for Drug Discovery<br />
124<br />
Co-Author(s)<br />
Chris Shumate,<br />
Paul B. Comita<br />
The drug discovery industry is in need of improved screening technologies that provide knowledge and foresight<br />
into success of new drug candidates. Currently most discovery efforts make use of fluorescence intensity for high<br />
throughput and high content screening (HTS/HCS). One of the more recent developments in optical imaging for<br />
complex biological systems is the use of fluorescence emission lifetime for imaging microscopy (FLIM). Fluorescent<br />
lifetime and polarization imaging of cells provide powerful tools for assays that use detection of the presence,<br />
quantity, and location of labeled molecules and their binding state. Incorporating these features into a fast and<br />
inexpensive commercial product is a significant technical challenge. Blueshift Biotechnologies is addressing<br />
this challenge by exploiting recent advances in laser sources, semiconductor inspection technology, fluorescent<br />
probes, and high content screening. Blueshift will present a novel cellular analysis platform to drive drug discovery<br />
research and HTS applications.<br />
8:15 am Wednesday, February 4 Emerging Technologies – Cell Based Screening Room A3<br />
James Costantin<br />
Molecular Devices Corp.<br />
1131 Orleans Drive<br />
Sunnyvale, California 94089<br />
Screening for Ion Channel Modulators Using the IonWorks HT System<br />
Co-Author(s)<br />
Shawn Handran, Andrew Wittel,<br />
Sven Brown, Nick Callamaras,<br />
Wilhelm Lachnit<br />
The IonWorks HT instrument is an automated high throughput voltage clamp platform that measures whole-cell<br />
currents from multiple cells in parallel using 384-well PatchPlates. This robust, turn-key platform performs highly<br />
consistent, single-point lead candidate screens for modulators of ion channel activity and has the fidelity required<br />
for IC50 determinations. Successful recordings have been obtained from a variety of cultured cell lines (e.g., CHO,<br />
CHL, HEK) expressing recombinant ion channels. For single-point screening, data was generated from compound<br />
plates prepared containing randomly distributed compounds as well as controls. Following the identification of<br />
active wells, an 8-point IC50 curve for each compound was obtained. Results are presented for compounds that<br />
affect hERG, Na+ and other voltage-gated ion channels. The IC50 curves generated from IonWorks HT are<br />
comparable to values obtained in parallel by conventional patch clamp electrophysiology. IonWorks HT provides<br />
an enormous opportunity to screen pharmaceutical compound libraries directly at the electrophysiological level for<br />
more cost-effective and accelerated identification of therapeutic ion channel targets.
8:30 am Wednesday, February 4 Emerging Technologies – Cell Based Screening Room A3<br />
Jeffrey Price<br />
University of California, San Diego<br />
9500 Gilman Drive<br />
La Jolla, California 92093-0412<br />
price@bioeng.ucsd.edu<br />
125<br />
Co-Author(s)<br />
Michael A. Mancini<br />
Baylor College of Medicine<br />
Susanne Heynen, Ivana Mikic, Tim Moran<br />
Q3DM, Inc.<br />
Dynamic Data Mining and High Throughtput Microscopy Improve Productivity of Assay<br />
Design and Screening<br />
Automated high throughput/high-resolution microscopy (0.5 to 0.95 NA lenses) creates a potentially overwhelming<br />
number of cellular and subcellular measurements. Cellular heterogeneity adds complexity, can hinder screen<br />
significance and complicate assay design. Dynamic data-driven mining creates defined subpopulations without<br />
physical sorting to facilitate rapid design and testing of new subcellular assays and decreasing preparation<br />
time. A large suite of cell-by-cell subcellular parameters enables rapid culling together the best fluorescence,<br />
morphometry, translocation and pattern measurements for a new assay. Here, the impact of dynamic data mining<br />
was established in development of a ligand-dependent androgen receptor (AR) trafficking assay. Transiently<br />
transfected GFP-AR and AR619 (an inactivating prostate cancer-associated point mutation) were introduced<br />
into HeLa cells and analyzed with the EIDAQ 100 high throughput microscopy (HTM) system. AR subcellular<br />
distribution was examined for nuclear translocation and subnuclear distribution patterns in response to an<br />
11-point dose response of agonist (R1881) or antagonists (estradiol, Casodex, OH-Flutamide), and demonstrates<br />
the advantage of data mining. AR subpopulations responded consistently to ligand concentration in nuclear<br />
translocation (agonist and antagonist for both AR and AR619). However, only R1881 induced appearance of large<br />
subnuclear foci in AR619. Rapid experimentation with various measurements also enabled assay development<br />
that was sensitive to subtle, ligand-dependent differences in subnuclear pattern of GFP-AR. Cell cycle phases and<br />
metabolic states are additional examples where subpopulation assays may enable more productive screening.<br />
Thus, while image-based subcellular imaging can at first appear to increase assay complexity, powerful dynamic<br />
data mining tools also enable rapid development of new subcellular assays.<br />
8:45 am Wednesday, February 4 Emerging Technologies – Cell Based Screening Room A3<br />
Steve Richmond<br />
Genetix Ltd<br />
Queensway, New Milton BH25 5NN United Kingdom<br />
steve.richmond@genetix.com<br />
Development of a Mammalian Cell Colony Imaging and Picking Robot: ClonePix<br />
Co-Author(s)<br />
Chris Mann, Sky Jiang,<br />
James Colehan, Sarah Stephen,<br />
Julian Burke<br />
Robotics for picking microbial colonies have been available for approximately 10 years and played a key role in<br />
the various genome sequencing projects. Up until now no equivalent systems have existed for handling colonies<br />
of mammalian cells in culture. The task is more challenging as they are more fragile, more prone to contamination<br />
and often grow adhered to a surface. We believe that ClonePix is the first robot to automate transfer of cultured<br />
mammalian cells. The system picks both adherent colonies and those grown in semi-solid media and transfers<br />
them to micro plates. Culture dishes to be processed are imaged using a high-resolution CCD camera. The image<br />
is analyzed and a user-defined clone pick list is generated. The selected clones are picked using a novel 8-channel<br />
head. Each of the channels fires independently, allowing for high throughput picking at rates of ~200 clones per<br />
hour. The location of each clone in the microplate is automatically logged for sample tracking. Sterility of the tips<br />
is achieved via an ethanol wash combined with a high-temperature dryer. External contamination is minimised by<br />
the HEPA filter with positive air pressure and a fully enclosed working area; which can be disinfected with a UV<br />
lamp prior to use. Applications of ClonePix include selection of hybridomas expressing monoclonal antibodies and<br />
selection of transformed cells.<br />
PODIUM ABSTRACTS
9:00 am Wednesday, February 4 Emerging Technologies – Cell Based Screening Room A3<br />
Rowan Stringer<br />
Novartis Horsham Research Centre<br />
Wimblehurst Road, Horsham<br />
West Sussex, Nr London, RH12 5AB United Kingdom<br />
rowan.stringer@pharma.novartis.com<br />
96-well Protein-binding Studies in Drug Discovery<br />
126<br />
Co-Author(s)<br />
Rachael Profit<br />
Sarah Beech<br />
Paul Nicklin<br />
The protein-binding properties of 95 commercially available drugs were assessed using a 96-well ultrafiltration<br />
device (Millipore Multiscreen Ultracel ® -PPB). This system has low non-specific binding, good intra- and interexperiment<br />
reproducibility and can be coupled with higher-throughput bioanalysis. Results generated using<br />
this new method had good agreement with literature values for these reference compounds (R2 – 0.89); e.g.,<br />
bupivacaine 93% (95%), alprazolam 69% (71%), betaxolol 44% (55%), trimethoprim 43% (37%) and tocainide<br />
10% (10%), literature values in parentheses. This plasma protein-binding assay offers advantages over single<br />
ultrafiltration devices and equilibrium dialysis methods in terms of speed, convenience and aut<strong>omation</strong> potential. It<br />
will be used to provide initial protein-binding results to support early drug discovery programs.<br />
9:15 am Wednesday, February 4 Emerging Technologies – Cell Based Screening Room A3<br />
Ben Tseng<br />
Maxim Pharmaceuticals<br />
6650 Nancy Ridge Drive<br />
San Diego, California 92121<br />
btseng@maxim.com<br />
Co-Author(s)<br />
Sui Xiong Cai, John Drewe,<br />
John Herich, Shailaja Kasibhatla<br />
A Drug Discovery Screen and Chemical-Genetic Approach for Novel Apoptosis Inducers<br />
We have developed and implemented a cell-based high throughput screen for anti-cancer drug discovery for<br />
the induction of apoptosis by measuring the downstream effector caspase 3. A proprietary pro-fluorescence<br />
substrate is used in the assay that is robust and suited for high throughput applications and has a Z´ factor of 0.8.<br />
A cell-based assay provides the advantages of identifying compounds that induce apoptosis through any one of<br />
multiple pathways as well as through new and novel targets. In addition, the compounds that are identified have<br />
bioactivity on cells. From the primary and confirmatory dose response screens, selected compounds are assayed<br />
and classified for their different effects on cell cycle progression or non-progression prior to activation of apoptosis.<br />
Additional molecular assays provide a refinement of these categories. These categorizations identify compounds<br />
that activate apoptosis through different and potentially novel mechanisms. Based on several selection criteria,<br />
the promising compounds are selected for analog synthesis to provide structure activity relationships as well as<br />
to improve compound properties. Analogs that meet these additional criteria are progressed to in vivo studies and<br />
to molecular target identification studies. We will present examples of two compounds discovered in our screens,<br />
their novel mechanisms of action, their in vivo activities, as well as target identification and validation strategies.<br />
Our chemical-genetic based program allows the discovery of novel pathways and targets for activating apoptosis<br />
and for the discovery of novel compounds that affect these pathways as potential anti-cancer agents.
9:30 am Wednesday, February 4 Emerging Technologies – Cell Based Screening Room A3<br />
Matthew Cook<br />
TTP LabTech<br />
Melbourn Science Park<br />
Melbourn, Herts, SG8 6EE United Kingdom<br />
matthew.cook@ttplabtech.com<br />
127<br />
Co-Author(s)<br />
Olivier Dery<br />
BD Biosciences Clontech<br />
Detection of BD Living Colors Novel Fluorescent Proteins Using the Acumen Explorer<br />
With the sequencing of many genomes now completed, biologists are faced with the challenge of deciphering the<br />
function and association of an immense number of resulting proteins. Understanding the specific processes and<br />
actions of proteins and chemicals that comprise the cells and tissues of an organism, will be a necessary next step<br />
to elucidating cellular function in healthy and disease states. Simultaneous and quantitative measurement of the<br />
level of expression, for tens of thousands of genes aids in the definition of a comprehensive molecular phenotype<br />
of cells and cellular processes. The Acumen Explorer exploits the power of fluorescence to monitor and<br />
elucidate subtle changes in inter and intracellular biochemical events. Proprietary Novel Fluorescent Proteins (NFP)<br />
from BD Biosciences Clontech include fluorescent proteins cloned from reef corals as well as from the jellyfish<br />
Aequorea coerulescens. From this portfolio, five distinct proteins, AcGFP1 (monomer), ZsGreen1, ZsYellow1,<br />
AsRed2 and DsRed2 are excited by a 488 nm Argon Ion laser. The resulting fluorescence emissions are directed to<br />
four photo multiplier tubes. Three colors/wavelengths regions can be scanned simultaneously thereby allowing true<br />
multiplexing with NFPs. The proprietary software algorithms permitted measurement in HEK293 cells of:<br />
• Single and mixed populations of cells expressing NFP genes.<br />
• Gene expression of two different colored NFP genes.<br />
• Detection of proteins linked to different colored NFP localized to cell compartments.<br />
The versatility of the Acumen Explorer in combination with BD Biosciences Clontech’s growing portfolio of NFPs<br />
provides an excellent tool for the investigation of functional genomic applications.<br />
9:45 am Wednesday, February 4 Emerging Technologies – Cell Based Screening Room A3<br />
Johannes Dapprich<br />
Generation Biotech, LLC<br />
32 Pin Oak Drive<br />
Lawrenceville, New Jersey 08648<br />
jdapprich@generationbiotech.com<br />
Co-Author(s)<br />
Cynthia Turino, Sarah Jones, Colleen Murphy, Nancy Murphy<br />
GenoVision, Inc.<br />
Automated Molecular Haplotyping Through Physical Separation of DNA<br />
Tine Thorbjornsen<br />
Qiagen AS<br />
Laurie Burdett, Marcello Fernandez-Vina<br />
C.W. Bill Young Marrow Donor Program<br />
We report the aut<strong>omation</strong> of a method, Haplotype-Specific Extraction (HSE), that allows the physical separation<br />
of diploid genomic DNA into its haploid components. Magnetic beads are selectively attached to polymorphic<br />
sites and used to isolate targeted, double-stranded DNA from a heterozygous mixture, thereby establishing<br />
individual patient’s haplotypes within hours without knowledge of familial information. Automated Haploseparations<br />
were carried out on several different liquid handling robots capable of handling 6, 48 and 96 samples<br />
in different formats. This enables efficient, parallel and reliable processing of multiple samples. Haplo-separated<br />
DNA is directly analyzed with kits and assays already in use for genotyping. The method was used on potential<br />
organ donor samples to separate parts of chromosome 6 containing the HLA (Human Leukocyte Antigen)<br />
locus. The highly polymorphic HLA-locus is routinely typed before transplantations can be carried out. Frequent<br />
recombination events throughout the evolution of the locus have led to numerous allele pair combinations that, in<br />
sum, lead to the same diploid genotype information as other allele pairs. HSE permits the unambiguous typing of<br />
such allele pair combinations that may otherwise fail to be resolved by conventional sequence-based typing (SBT)<br />
and sequence-specific oligonucleotide probes (SSOP). The purification of alleles allows the identification of known<br />
or new haplotypes directly from genomic DNA.<br />
PODIUM ABSTRACTS
10:30 am Wednesday, February 4 Emerging Technologies – Hardware Room A3<br />
Jason Armstrong<br />
Polymerat<br />
Unit 4 / 26 Brandl Street<br />
Eight Mile Plains, 4113 Australia<br />
jason@cmcapital.com<br />
Optimizing Surfaces for Assays in Plates, on Luminex Beads and Microarrays Using<br />
Combinatorial Chemistry Customized Surface Coatings<br />
Surfaces are a current limitation to the advancement of a number of life science applications. Polymerat has<br />
developed methods that enable the combinational assembly of molecular surface coatings that will allow structure<br />
property relationships for surface-proteins interactions and hence lead to interfaces that permit the biological<br />
components of assays to perform at their maximum efficiently. The polymer synthesis methods are based on a<br />
synthon intermediate, analogous to a key intermediate in small molecule synthesis. Polymerat’s technology has<br />
broad applications, however, three priority areas are being pursued: Assays, Biochips and Bioseparations. The<br />
technology is layered on top of non-reactive substrates, such as plastics and glass, in an array of formats ranging<br />
from microscope slides, encoded beads and microtitre plates. From a single intermediate, it is possible to generate<br />
a wide diversity of molecular coatings by chemical transformation of the reactive monomer in the thin grafted layer.<br />
For instance, employing commercially available building blocks of amines and carboxylic acids, one can readily<br />
generate thousands of molecular coatings that are evaluated through high throughput screening. When combined<br />
with further derivitization, where each surface can itself be propagated, extensive surface coating diversity is<br />
readily generated. In particular, Polymerat’s technology allows for the manipulation of proteins in their native<br />
state for presentation, isolation and screening. Such ability has direct application in proteomics discovery where<br />
strategies for a biomimetic approaches to selective capture and presentation of proteins is gaining popularity.<br />
Polymerat currently has a number of product development partners and is looking to integrate its technology into<br />
other platform technologies.<br />
10:45 am Wednesday, February 4 Emerging Technologies – Hardware Room A3<br />
Prabhu U. Arumugam<br />
University of Arkansas<br />
101 Chemistry Building<br />
Fayetteville, Arkansas 72701<br />
parumug@uark.edu<br />
Development of Reduction-Oxidation Magnetohydrodynamic Devices With Integrated<br />
Permanent Magnets<br />
128<br />
Co-Author(s)<br />
Ingrid Fritsch<br />
We will report investigations into the effects of parameters that will enable the development of an effectual design<br />
methodology for reduction-oxidation (redox) magnetohydrodynamic (MHD) pumps and stirrers. The interaction of<br />
an electrically conducting fluid with a magnetic field may be described by MHD theory. This theory describes a<br />
force on current-carrying species governed by the right-hand rule, perpendicular to both the electric and magnetic<br />
fields. Our approach is to use redox species in solution as current-carrying medium and apply either an external<br />
or internal magnetic field. The advantages of using MHD as a microfluidic system over other methods such as<br />
mechanical and electrokinetic pumping are no moving parts, low operating voltages (1 mV-2 V), continuous and<br />
reversible flow, and the flexibility of choosing from a wide variety of parameters to control. We will present the<br />
results of microelectrodes embedded in magnets, which show that sufficient MHD effects can be achieved even<br />
at low magnetic fields (0.13 T). Progress toward the use of simulations to define the optimal design parameters<br />
for fluidics, such as aspect ratio of channels, various geometries of electrodes and magnets, concentration of<br />
redox species, roughness of channel surfaces, magnetic flux density and its distribution, and heat gradients will be<br />
reported. In addition, the suitability of integrating permanent magnets onto microfluidic channels for portability will<br />
be discussed.
11:00 am Wednesday, February 4 Emerging Technologies – Hardware Room A3<br />
Brian Cunningham<br />
SRU Biosystems<br />
14A Gill Street<br />
Woburn, Massachusetts 01801<br />
bcunningham@srubiosystems.com<br />
Label-Free Assays Using the BIND System<br />
129<br />
Co-Author(s)<br />
Peter Li, Stephen Schulz,<br />
Bo Lin, Cheryl Baird<br />
Screening of biochemical interactions becomes simpler, less expensive, and more accurate when labels, such as<br />
fluorescent dyes, radioactive markers, and colorimetric reactions, are not required to quantify detected material.<br />
SRU Biosystems has developed a biosensor technology that is manufactured on continuous sheets of plastic<br />
film, and incorporated into standard microtiter plates and microarray slides to enable label-free assays to be<br />
performed with high throughput, high sensitivity, and low cost per assay. The biosensor incorporates a narrowband<br />
reflectance filter, in which the reflected color is modulated by the attachment/detachment of biochemical material<br />
to the surface. The technology offers 4-orders of linear dynamic range, and uniformity within a plate with a<br />
coefficient of variation of 2.5%. Two readout instruments are demonstrated: a plate reader capable of reading one<br />
data point per well in 96- or 384-well microplates, and a high performance imaging plate reader. Both instruments<br />
are capable of reading an entire biosensor plate in 15-30 seconds and integrating with robotic microplate<br />
handlers. Using conventional biochemical immobilization surface chemistries, a wide range of assay applications<br />
are enabled. Small molecule screening, cell proliferation/cytotoxicity, enzyme activity screening, protein-protein<br />
interaction, cell membrane receptor, and microarray imaging are among the applications demonstrated.<br />
11:15 am Wednesday, February 4 Emerging Technologies – Hardware Room A3<br />
Martin Dufva<br />
Mikroelektronik Centret<br />
Technical University of Denmark<br />
Building 345 East<br />
Kongens Lyngby, Denmark<br />
mdu@mic.dtu.dk<br />
Co-Author(s)<br />
Michael Stangegaard, Per Jensen Mikkelsen,<br />
Louise Dahl Christensen, Claus BV Christensen<br />
A Flexible Substrate for DNA Microarray Hybridization and Detection Using a<br />
Business Card Scanner<br />
DNA and protein microarrays are powerful tools for both protein and mRNA expression profiling, respectively, due<br />
to the immense parallelism of the analysis methods. Microarrays can furthermore be used for diagnosing bacteria<br />
and quantifying small molecule contaminations, like pesticides in water, with increased sensitivity compared<br />
to traditional methods. Traditional microarray technology, however, often relies on bulky equipment. Here we<br />
present a flexible (100 micrometer thin) microarray substrate that can be used for detecting microarray-analyte<br />
interactions using an ordinary business card scanner for signal generation. Hybridized DNA was stained by a<br />
color reaction mediated by alkaline phosphatase prior to scanning. The results were comparable with the results<br />
obtained using a desktop scanner and showed that hybridization of 50 pM target could be detected using this<br />
alternative detection system. Detection of immobilized biotinylated DNA on the microarray surface indicated that<br />
2–3 amol of biotinylated DNA could be detected. The business card scanner (weight approx. 200 gram) only needs<br />
an USB port on a portable computer and no AC power to function, making this detection system truly portable.<br />
Furthermore, the thin and flexible microarray substrate may be a more appropriate solid support than glass for<br />
integrating microarrays with microfluidics.<br />
PODIUM ABSTRACTS
11:30 am Wednesday, February 4 Emerging Technologies – Hardware Room A3<br />
Gabriele Gradl<br />
Evotec Technologies<br />
Invalidenstr. 42<br />
Berlin, 10115 Germany<br />
gabriele.gradl@evotec-technologies.com<br />
Merging Highest Resolution and High Speed: Instrumentation for HTS Cell Assays and<br />
Image Activated Cell Sorting<br />
In the post genomic era the growing needs of drug discovery assays must cover novel approaches to cellular<br />
data. Clever strategies for the whole process of target identification, target validation, development of cell lines<br />
for screening and follow-up testing are required. Images have to be acquired fast and from large nu<strong>mbers</strong> of<br />
cell samples. Powerful and intelligent algorithms are required for image processing for the different assay types.<br />
Solutions will be presented which remove the limitation terms of generating data from imaging spans and the<br />
laborious work of cloning mammalian cell lines for use in cell assays. They will serve for applications in functional<br />
proteomics, high throughput biology and drug discovery. Strictly confocal imaging allows to exploit cellular<br />
information at the subcellular level and at the same time it provides information on the status of the cell while<br />
incubated with the compound of interest (e. g., integrity, next-neighbour contacts, toxic effects, apoptopic effects).<br />
A highly flexible scripting language for image analysis is used which enables the user to customize the software<br />
towards the specific needs of the respective assay fast assay development. More than 100,000 data points<br />
per day are generated in HTS applications. Several applications are presented. Pure cell clones are generated<br />
with high efficiency using superior micro-fluidic and contact-free manipulation methods. The manual and timeconsuming<br />
steps of growing, identifying, isolating and analysing mammalian cell clones are automated and the<br />
whole procedure is shortened from weeks to days.<br />
11:45 am Wednesday, February 4 Emerging Technologies – Hardware Room A3<br />
Paul Hensley<br />
Microplate Aut<strong>omation</strong><br />
560 Fellowship Road, Suite 211<br />
Mt. Laurel, New Jersey 08054<br />
paul_hensley@microplateaut<strong>omation</strong>.com<br />
TipCharger ® Cleaning Technology: Status and Laboratory Performance of the Technology<br />
Microplate Aut<strong>omation</strong>’s cleaning technology uses safe, low temperature atmospheric plasma to break down<br />
organic compounds into atomic units and combines them with oxygen. Data will be presented to illustrate the<br />
effectiveness of the process for removing low molecular weight compounds, larger macromolecules, DNA/RNA<br />
and living organisms from fluid handling systems. The talk will include examples of typical integration issues an<br />
end user faces when retrofitting the TipCharger ® hardware into various aut<strong>omation</strong> platforms and engineering<br />
solutions that have been developed to facilitate rapid deployment. There will also be a discussion of uses for the<br />
hardware’s optical spectrophotometer and optional Ramen spectrophotometer.<br />
130
12:00 pm Wednesday, February 4 Emerging Technologies – Hardware Room A3<br />
Jeffrey Karg<br />
Boston Innovation, Inc.<br />
101 Rogers Street, #216<br />
Cambridge, Massachusetts 02142<br />
jkarg@bostoninnovation.com<br />
SmartPlate Implementation and Return on Investment Examples for Compound Management<br />
Assay miniaturization has pushed the limits of compound dispensary logistics. 384 and 1536 well screening<br />
requires high quality compounds accurately, reliably, and cost-effectively dispensed into a wide range of assay<br />
formats. This time-consuming and wasteful step is eliminated with SmartPlate shipping, storing, and dispensing<br />
technology. This presentation will highlight how SmartPlate works, as well as providing details on integration with<br />
a variety of liquid handling equipment. In addition, SmartPlate’s return of investment for focused libraries (5–100K<br />
compounds) and full size libraries will be explored.<br />
12:15 pm Wednesday, February 4 Emerging Technologies – Hardware Room A3<br />
Hakki Unver<br />
Zymark Corporation<br />
68 Elm Street<br />
Hopkinton, Massachusetts 01748<br />
hounver@hotmail.com<br />
Using Data Collected in Real Time From Liquid Transfer Operations to Audit and<br />
Enhance Performance<br />
131<br />
Co-Author(s)<br />
Gregory Wendel, John Howland<br />
Zymark Corporation<br />
Mary Jo Wildey<br />
Johnson & Johnson PRD<br />
Channel-by-channel performance data collected from a liquid transfer device can be utilized to document transfers<br />
for regulatory agencies, eliminate false assay results due to transfer failures, provide baselines for continuous<br />
improvement, and allow for immediate corrective action in the transfer method. This presentation describes<br />
the results of experiments with a 96-channel liquid transfer device that measures in real time and reports data<br />
about the volume transferred and information about the quality of the transfer for each channel. The experiments<br />
characterized the internal operational data to evolve quality information for detecting and reporting common faults<br />
such as partially clogged tips and unexpected air in the channels. Development work explored ways to make the<br />
information available to the method developer for enabling real time feedback control of the transfer method. Also,<br />
the work explored ways that the device could export the information for bioinformatics data analysis as well as<br />
documentation for regulatory agencies.<br />
PODIUM ABSTRACTS
3:30 pm Wednesday, February 4 Emerging Technologies – Aut<strong>omation</strong> Systems Room A3<br />
Bruce Seligmann<br />
High Throughput Genomics, Inc.<br />
6296 E. Grant Road<br />
Tucson, Arizonia 85712<br />
bseligmann@htgenomics.com<br />
The ArrayPlate: A Novel, High Throughput, Multiplexed, mRNA Assay for Toxicological<br />
Research and Development<br />
High Throughput Genomics (HTG) has developed an easy-to-implement, industrial scale gene expression assay<br />
in a two-stage microplate multiplexed array format that eliminates the need for RNA extraction, purification,<br />
and amplification. The assay produces sensitive, reproducible, repeatable from day-to-day, and lab-to-lab,<br />
quantitative gene expression data with CV’s in the range of 10 percent or less. Utilizing transcriptomics technology,<br />
toxicologists can now overcome some of the problems with studying gene expression using chip or amplification<br />
technologies, particularly when analyzing in vivo animal studies. HTG’s ArrayPlate, when compared with<br />
current chip technologies, allows toxicologists to examine small gene expression changes and develop a highly<br />
reproducible pattern of gene expression that may indicate a toxic event and assemble highly accurate dose/<br />
response curves. The technology’s multiplexed gene expression capability makes the assay a high throughput<br />
screening tool that can be deployed in early stage drug development and compound screening efforts. No RNA<br />
purification or sample amplification is necessary to obtain excellent data making the ArrayPlate a highly cost<br />
effective platform for compound testing. Thousands of samples can be analyzed in one week. HTG’s technology<br />
is currently in use by pharmaceutical companies (such as Johnson & Johnson, Celgene) in research and<br />
development, discovery, toxicology, metabolism, diagnostics, clinical trial monitoring, and many other life science<br />
applications.<br />
3:45 pm Wednesday, February 4 Emerging Technologies – Aut<strong>omation</strong> Systems Room A3<br />
David Huber<br />
Stanford University<br />
Building 500<br />
Stanford, California 94305-3030<br />
david.huber@stanford.edu<br />
Temperature Gradient Focusing, Modeling and Experiments<br />
132<br />
Co-Author(s)<br />
Juan G. Santiago<br />
A key challenge yet to be addressed by miniaturized bioanalytical devices is the detection of analytes with<br />
nanomolar or lower initial concentrations in volumes of order one microliter or less. Temperature gradient<br />
focusing (TGF) is an emerging analytical technology that simultaneously concentrates and separates charged<br />
species according to their electrophoretic mobilities. In TGF, an axial temperature gradient is applied along<br />
an electrophoretic channel. Within the channel, the local electric field is inversely proportional to conductivity,<br />
which in turn is a function of local viscosity and ion density. By selecting a buffer with a temperature dependent<br />
conductivity, we create an electric field gradient that causes a decrease in electrophoretic mass flux along one<br />
direction in the channel. We then impose a net bulk flow in the opposite direction. Species in the system focus at<br />
points where the local, area-averaged liquid velocity and electrophoretic velocity sum to zero. Dispersion in TGF<br />
results from a combination of molecular diffusion and advective dispersion. Advective dispersion, the dominant<br />
component, is caused by both externally-imposed and internally-generated pressure gradients. We leverage a<br />
dispersion model similar to classical Taylor dispersion analysis to produce a one-dimensional convective diffusion<br />
equation in terms of an axially-dependent dispersion coefficient. In this work, we compare the dispersion model<br />
with results from our temperature gradient flow system and characterize the focusing and dispersion behavior. The<br />
goal of the study is to determine optimal parameters for TGF focusing and separation.
4:00 pm Wednesday, February 4 Emerging Technologies – Aut<strong>omation</strong> Systems Room A3<br />
Sanjaya Joshi<br />
Userspace Corporation<br />
11118 NE 141 Place<br />
Kirkland, Washington 98034<br />
sanjay@userspace.com<br />
An Automated Flow Cytometry Quality Protocol and Workflow System for Managing<br />
Instruments, Samples, and Data<br />
133<br />
Co-Author(s)<br />
Michael R. Loken<br />
Hematologics, Inc.<br />
Flow Cytometry is gaining significance outside the research laboratory as a high-resolution clinical tool for the<br />
detection, classification, staging, and recurrence of Hematologic neoplasms. Intensity relationships between<br />
antigens is proving to be an important discriminator of normal and aberrant hematopoietic cells. Userspace<br />
Corporation and HematoLogics, Inc. have been collaborating to create a web-service based real-time workflow<br />
system combined with a unique performance assessment for the instrument calibration based on invariant<br />
biological markers. A high resolution instrument validation protocol can be established using lymphocyte CD4<br />
fluorescence intensity. In a study of normal adult blood using these QC procedures, the intensity of CD4 on<br />
lymphocytes was found to be essentially invariant for 21 individuals assayed on the two instruments collected<br />
over a period of 8 months. These results demonstrate that in a data space with a dynamic range of 4 decades, the<br />
biological variation of the mean intensity from individual to individual for this one antigen is less than the variability<br />
of expression within the individual. The biological expression of this antigen becomes an independent biological<br />
standard. This QC procedure is a rapid means of assessing instrument performance. This quality program<br />
combined with the tracking and audit of samples and patient records securely in real-time allows both clinical<br />
laboratories and research laboratories conducting clinical trials to remotely track and analyze the FCS data format<br />
(converted into XML for data stream based interpretation, analysis and report generation). Templates can be built<br />
around this system for various quality and regulatory standards.<br />
4:15 pm Wednesday, February 4 Emerging Technologies – Aut<strong>omation</strong> Systems Room A3<br />
Emir Osmanagic<br />
Scinomix<br />
4069 Wedgeway Court<br />
Earth City, Missouri 63045<br />
eosmanagic@scinomix.com<br />
Vertical Integration Platform<br />
Co-Author(s)<br />
Russell Leeker<br />
Traditionally, companies have designed laboratory aut<strong>omation</strong> systems to operate with few significant changes<br />
during assay production. The inflexibility of this approach is the primary reason companies are supporting<br />
development of more versatile systems. The Vertical Laboratory Integration Platform (VIP) is a framework for<br />
distributed modular aut<strong>omation</strong> for laboratory processes. Modularity of the components, highly flexible product<br />
transport mechanisms, and a high level of distributed intelligence are key characteristics of the VIP. Laboratories<br />
can realize an excellent return on investment through the VIP’s ability to adapt to the quick and rapid changes of<br />
lab processes. The first version of the VIP has been developed for Cell-Based Assays incorporating incubation,<br />
liquid handling, washing and plate sealing on plug-and-play shelves under environmental controlled conditions.<br />
The system transports any height SBS standard footprint plates to and from instruments in a vertical configuration,<br />
saving valuable laboratory space. When a process changes and an additional or different instrument is required a<br />
plug and play shelf can be removed and reconfigured then plugged back into the VIP framework.<br />
PODIUM ABSTRACTS
4:30 pm Wednesday, February 4 Emerging Technologies – Aut<strong>omation</strong> Systems Room A3<br />
Bradley Mikesell<br />
Pfizer Global R&D<br />
10777 Science Center Road<br />
San Diego, California 92121<br />
bradley.mikesell@pfizer.com<br />
Automated Accurate Mass Analysis Using FTICR Mass Spectrometry<br />
134<br />
Co-Author(s)<br />
Manuel Ventura, Terri Quenzer,<br />
Ben Bolanos, Michael Greig<br />
High-resolution mass spectrometry can be utilized as a fast, effective means of confirming the empirical formula<br />
of most pharmaceutical compounds or aiding in the elucidation of unknown structures. Advantages of Fourier<br />
transform ion cyclotron resonance mass spectrometry (FTICR-MS) for accurate mass assignment include superior<br />
mass accuracy, high resolution, high sensitivity, high precision, and rapid analysis. FTICR-MS is also well-suited<br />
for MS n experiments due to nondestructive ion detection. Here we describe an automated system to acquire small<br />
molecule accurate mass data for a wide variety of compounds for drug discovery using a high-resolution FTICR-<br />
MS instrument. An external standard consisting of three compounds of various masses is introduced with each run<br />
to ensure a reliable calibration, generally to within 2 ppm accuracy. To correct for variable sample concentrations,<br />
we have developed an active script to optimize ion population by normalizing ion accumulation times. Samples<br />
which fail to yield adequate ion signal intensity under a standard positive mode electrospray method are evaluated<br />
subsequent to the batch run. The ionization source on this system is readily switched out so that either APCI<br />
(atmospheric pressure chemical ionization) or APPI (atmospheric pressure photoionization) sources may be used<br />
for samples which are better suited to those techniques. This system thereby enables automated detection/<br />
confirmation of nearly all compound classes submitted providing valuable structure validation for drug discovery<br />
projects with rapid turnaround. It is also highly useful in these efforts for identification of unknown species.<br />
4:45 pm Wednesday, February 4 Emerging Technologies – Aut<strong>omation</strong> Systems Room A3<br />
Donald Mossman<br />
CPC-Cellular Process Chemistry, Inc.<br />
One Broadway Street, Suite 600<br />
Blackstone, Massachusetts 02142<br />
mossman@cpc-net.com<br />
Sequential Organic Synthesis as a New Approach in Drug Discovery<br />
Scientists in drug discovery have been constantly challenged to improve the lead generation process. Shorter<br />
product life spans and increasingly tight specifications are accompanied by ever increasing demands for larger<br />
nu<strong>mbers</strong> of candidates and more specific functionality each of them. With these demands also comes the need for<br />
reproducibly high purity compounds manufactured in a safe manner with complete traceability. In their attempts<br />
to achieve this companies find that there is excessive resource drainage as the iterative cycles of adaptation of<br />
chemistries to an increasing variety of constraints. CYTOS ® Continuous Chemistry establishes a revolutionary new<br />
alternative enabling companies to conduct their chemistries from early research through to production using the<br />
same technology and synthesis process. CYTOS ® can provide advanced technology for improving and optimizing<br />
chemical synthesis through continuous chemical synthesis and the use of microreaction technology. Microreaction<br />
technology already is inducing a paradigm shift by demonstrating the advantages of continuous processing<br />
(better chemistry, faster development and higher R&D throughput) over the more conventional batch processing.<br />
This technology eliminate scale-up problems, due to the fact that parallelized arrays allows for the production of<br />
chemicals and pharmaceuticals from milligram to Kg scale and into full production quantities all in the exact same<br />
environment using the exact same reactions.
5:00 pm Wednesday, February 4 Emerging Technologies – Aut<strong>omation</strong> Systems Room A3<br />
Jaymie Sawyer<br />
Dyax Corporation<br />
109754 Torreyana Road, W-1<br />
San Diego, California 92121<br />
jsawyer@dyax.com<br />
Managing Automated RFLP Analysis in Phage Display Antibody Screening<br />
135<br />
Co-Author(s)<br />
I-wei Feng, Lee A. Morganelli,<br />
Rosanto Paramban, Roger Dettloff,<br />
Irina Mineyev, Paul Kotturi<br />
Caliper Technologies Corporation<br />
Automated screening of Dyax’s proprietary human Fab library is currently in use to discover new antibodies<br />
and develop research reagents in collaboration with BD Biosciences. PCR fingerprinting is a useful tool in the<br />
analysis of clones derived from large libraries containing similar sized inserts. The fingerprints allow us to assess<br />
the diversity in pools of selected phage before moving into high throughput screening, or to avoid repeated<br />
expression and purification of the same clone. Vector-specific primers amplify the insert which is then digested<br />
with a frequently cutting restriction enzyme. Typically, gel electrophoresis is then used to visualize RFLPs. The<br />
Caliper AMS 90 SE system allows 96-384 digested DNA samples to be analyzed without running or photographing<br />
gels. The series of DNA fragments within a given sample are identified by LabChip HT software. To fully exploit<br />
fingerprinting, the data output must allow comparison of digests run at different times and facilitate correlation of<br />
clone identity with functional assays. These final data handling procedures are tedious and not automated. We<br />
developed a software tool to analyze the results from an AMS 90 SE and automate the final clone identification.<br />
DNA fragments between 50 and 500 base pairs are binned into groups between 15 and 20 bps. The peaks within<br />
a group are assigned a letter of the alphabet, resulting in a clone ‘name’. The resulting clone names can then be<br />
exported and correlated with functional assays such as expression levels and ELISA performance to identify new<br />
antibodies generated by phage display.<br />
5:15 pm Wednesday, February 4 Emerging Technologies – Aut<strong>omation</strong> Systems Room A3<br />
David Semin<br />
Amgen, Inc.<br />
One Amgen Center Drive, Mailstop 29-M-B<br />
Thousand Oaks, California 91320<br />
dsemin@amgen.com<br />
The Next Generation of a Compound Management System in Drug Discovery<br />
Co-Author(s)<br />
Janet Cheetham, Stewart Chipman,<br />
Peter Grandsard, Colin McRavey,<br />
Sabrina Park, Jim Petersen<br />
As the competition in drug discovery intensifies companies need to become more operationally efficient and to<br />
maximize return on investment. Since the emergence of automated compound storage and retrieval systems in the<br />
1990s there has been considerable progress on overall compound management strategies to facilitate compound<br />
flow throughout research operations. Some of these recent strategies have evolved around the new market of<br />
small to medium sized storage and retrieval systems. In an effort to fully enable Amgen’s global lead discovery<br />
efforts we are developing a compound management system based on a modular and multi-tier approach with<br />
the concept of archival and operational compound stores. The modularity enables flexibility and incorporation<br />
of new technology platforms, while the multi-tier approach enables redundant and priority storage formats. A<br />
system of operational stores will fuel the chemistry and high throughput screening activities, while an archival<br />
store will be used ensure long-term compound quantity and quality. A global software infrastructure solution<br />
with generic interfaces to the modular components will be presented. The infrastructure is designed to enable<br />
excellence at compound collection stewardship while maintaining flexibility for change in business processes<br />
and the incorporation of new technology platforms. This drive towards the next generation system built upon<br />
these hardware and software concepts should enable a compound management system in drug discovery to be<br />
operationally efficient and effectively stay off the critical path and timeline for research programs.<br />
PODIUM ABSTRACTS
8:00 am Thursday, February 5 Emerging Technologies – National Lab Tech Transfer Room A3<br />
Erica Briggs<br />
Los Alamos National Laboratory, MS C333<br />
Los Alamos, New Mexico 87544<br />
eab@lanl.gov<br />
An Introduction of Technology Transfer With the National Laboratories<br />
The Technology Transfer Session will provide a forum for representatives from the DOE national laboratories to<br />
educate the audience about technology transfer mechanisms for moving science from the national laboratories to<br />
commercial markets. Laboratory presentations will focus on state-of-the-art capabilities in the areas of analytical<br />
instrumentation, high throughput screening/proteomics and computational software that are available for licensing<br />
and/or research collaboration. The session will begin with an overview of the basic missions of each national<br />
laboratory and a general description of the science currently being done at those institutions. Each participating<br />
laboratory will also have time to briefly highlight some of the relevant technologies being developed at their facility.<br />
We will also talk about some of the technology transfer success stories at each of the national labs. The five<br />
laboratories will all have approximately 15 minutes to present this information. Following the introductions of each<br />
laboratory, we will begin a presentation of technology transfer mechanisms at DOE national laboratories. This<br />
discussion will cover different structures for licensing technologies from the laboratories, arranging for collaborative<br />
research agreements, and initiatives for the commercialization of technologies from the laboratory. We will also<br />
address intellectual property issues as pertains to the above mentioned mechanisms, as well as work funded by<br />
outside parties at the labs, and user facilities at the labs. Open Questions and Answers: The final 20 minutes of the<br />
session will be an open forum for questions from the audience. At that time, we will be able to answer questions<br />
about technologies presented during the session, as well as mechanisms for technology transfer.<br />
8:15 am Thursday, February 5 Emerging Technologies – National Lab Tech Transfer Room A3<br />
Steve Lake<br />
Argonne National Laboratory<br />
9700 S. Cass Avenue<br />
Argonne, Illinois 60439<br />
slake@anl.gov<br />
Technology Transfer at Argonne National Laboratory<br />
136
8:30 am Thursday, February 5 Emerging Technologies – National Lab Tech Transfer Room A3<br />
Erica Briggs<br />
Los Alamos National Laboratory, MS C333<br />
Los Alamos, New Mexico 87544<br />
eab@lanl.gov<br />
Technology Transfer at Los Alamos National Laboratory<br />
8:45 am Thursday, February 5 Emerging Technologies – National Lab Tech Transfer Room A3<br />
Pam Seidenman<br />
Lawrence Berkeley National Laboratory<br />
1 Cyclotron Road MS 90R1070<br />
Berkeley, California 94720-8125<br />
psseidenman@lbl.gov<br />
Technology Transfer at Lawrence Berkeley National Laboratory<br />
137<br />
PODIUM ABSTRACTS
9:00 am Thursday, February 5 Emerging Technologies – National Lab Tech Transfer Room A3<br />
Bruce Harrer<br />
Pacific Northwest National Laboratory<br />
P.O. Box 999 / K9-78<br />
Richland, Washington 99352<br />
bruce.harrer@pnl.gov<br />
Technology Transfer at Pacific Northwest National Laboratory<br />
9:15 am Thursday, February 5 Emerging Technologies – National Lab Tech Transfer Room A3<br />
Laura Santos<br />
Sandia National Laboratories<br />
P.O. Box 5800 MS1413<br />
Albuquerque, New Mexico 87185-1413<br />
lesanto@sandia.gov<br />
Technology Transfer at Sandia National Laboratories<br />
138
10:30 am Thursday, February 5 Emerging Technologies – IT Informatics Room A3<br />
Larry Arnstein<br />
Teranode Corporation<br />
2815 Eastlake Avenue E.<br />
Seattle, Washington 98102<br />
larrya@teranode.com<br />
Design Tools: A New Approach to Computing in Life Sciences<br />
139<br />
Co-Author(s)<br />
Zheng Li<br />
Neil Fanger<br />
Research and development in life sciences must scale beyond the ability for one group, division, or even company<br />
to own all of the required scientific, laboratory, and information technology resources. Instead, new languages<br />
and tools are needed that allow individuals and groups to make specific contributions in the context of a large<br />
scale effort within or across enterprises. These new languages and tools must have broad applicability to foster<br />
heavy re-use of IT investment; and they must incorporate conceptual models and physical form factors that are<br />
appropriate for classically trained scientists and technicians. Pervasive and Ubiquitous Computing is a branch<br />
of computer science that aims to remove barriers, both physical and intellectual, to effective use of computing<br />
technology. On the strength of two technologies conceived at the University of Washington, Teranode Corporation<br />
has created a new category of software called “Design Tools for Life Sciences”. This technology integrates<br />
modeling of complex biological systems with design, execution, and documentation of laboratory procedures<br />
using a straight-forward graphical language that is backed by a pervasive computing infrastructure. Individuals can<br />
now communicate about outcomes and predictions with little to no extra effort, and IT professionals can create a<br />
wide variety of specific applications at low cost with a high degree of interoperability.<br />
11:00 am Thursday, February 5 Emerging Technologies – IT Informatics Room A3<br />
Eric Jones<br />
Enthought, Inc.<br />
515 Congress Avenue<br />
Austin, Texas 78701<br />
eric@enthought.com<br />
Python for Scientific Computing – An Open Source Solution<br />
Python is a well-designed general purpose programming language used in a variety of domains from powering<br />
web-sites (Google, Bank of America, NATO, and many others) to processing the data and images from the Hubble<br />
Telescope (STScI). Python has emerged as an excellent choice for scientific computing because of its simple<br />
syntax, ease of use, and elegant multi-dimensional array arithmetic. Python’s interpreted evaluation allows it to<br />
serve both as the development language and the command line environment in which to explore data. Python also<br />
excels as a “glue” language that joins multiple legacy codes written in different languages together – a common<br />
need in the scientific arena. This talk provides an overview of Python and reviews the wealth scientific tools<br />
available and how they pertain to laboratory aut<strong>omation</strong>. Some of the tools discussed include:<br />
• Numeric – Support for array (vectorized) arithmetic.<br />
• SciPy – Scientific Algorithms including Linear Algebra, signal processing, etc.<br />
• Chaco – Cross-platform plotting and visualization tools.<br />
Python and these associated tools share the same Open Source development model that has propelled the Linux<br />
operating system to notoriety. We will briefly discuss how open source methodologies can be used across an<br />
industry or within a company to create lower cost, quality software.<br />
PODIUM ABSTRACTS
11:30 am Thursday, February 5 Emerging Technologies – IT Informatics Room A3<br />
Gerald Knoll<br />
Fraunhofer Institute Manufacturing Engineering and<br />
Aut<strong>omation</strong> (IPA)<br />
Nobelstrasse 12<br />
Stuttgart, D-70569 Germany<br />
gerald.knoll@ipa.fhg.de<br />
140<br />
Co-Author(s)<br />
Johann Dorner, Frank Bölstler,<br />
Joachim Seidelmann<br />
Universal and Mobile Messaging Framework M2A “Message to Anywhere” for Laboratory<br />
Aut<strong>omation</strong><br />
This paper presents a software prototype for an universal and mobile Messaging Framework M2A “Message<br />
to Anywhere”. Access on actual laboratory data is an essential necessity in laboratory aut<strong>omation</strong>. This is how<br />
today’s laboratories are able to meet requirements as:<br />
• Faster changing processes with more reduced quantities<br />
• Access on various kinds of data<br />
• Configuration of laboratories distributed worldwide<br />
• Increase availability of laboratory equipment<br />
Mobility is an important factor in the cleanroom industry. Laboratory apparatus become much more flexible if they<br />
possess a mobile control (e.g., PDA, web-panel or cell phone). As a result, laboratory personnel need to coat less<br />
often due to the fact that an apparatus in a cleanroom can be controlled from any point. The time saved easily<br />
amounts to many hours per month. Furthermore, the risk of a probe becoming contaminated during its process<br />
is also reduced. In bio-engineering laboratories, the contamination hazard for laboratory personnel through lifethreatening<br />
substances can also be limited considerably by using a mobile control. The M2A-Framework consists<br />
of a communication layer complying with current standards. By using the buy-and-run principle, no software<br />
needs to be installed on the mobile end-device. A generic adapter permits the rapid linking of devices, equipment,<br />
and applications to the communication layer. A visualization component which is independent of the input/output<br />
device portrays directly the semantic model of the generic adapter. Therefore, client information as user manuals,<br />
ERP system order data or statically performance data are on hand, beside actual process data.
1:30 pm Thursday, February 5 Emerging Technologies – Performance Metrics Room A1<br />
John Bradshaw<br />
Artel, Inc.<br />
25 Bradley Drive<br />
Westbrook, Maine 04092<br />
jbradshaw@artel-usa.com<br />
141<br />
Co-Author(s)<br />
Alex L. Rogers, Tanya R. Knaide,<br />
Richard H. Curtis, George Rodrigues<br />
A Multichannel Volumetric Verification (MVV) System for Ensuring the Accuracy and Precision<br />
of Liquid Delivery<br />
Building upon their expertise in liquid delivery verification and their proprietary ratiometric photometry, Artel has<br />
developed a system that overcomes the limitations of other methods (e.g., gravimetric, fluorimetric, or single dye<br />
photometric) designed to calibrate automated liquid handling equipment. By measuring the absorbance ratio of<br />
two photometric dyes, the MVV system determines both accuracy and precision of volume delivery from various<br />
types of multichannel liquid handling equipment (both automated instrumentation as well as manual pipettes)<br />
with 8-, 12-, or 96-tips operating over a volume range from 2 to 200 mµL. A suggested robust test protocol of<br />
nine volumes over the working volume range requires from as little as one to at most three hours to perform,<br />
depending on the speed of the liquid handler being validated. As well as providing speed, ease-of-use and high<br />
performance in assessing accuracy and precision, the MVV system provides traceability to international standards<br />
which answers regulatory compliance requirements and ensures comparable inter-laboratory results. In-house data<br />
collected during the development of the MVV system, which shows good agreement with other approaches for<br />
determining liquid delivery, will be presented as a validation of the dual-dye photometric approach. Field testing of<br />
the MVV system on various makes of automated equipment will also be presented, further verifying this approach<br />
as a fast and reliable method for determining volume delivery performance. Finally, data demonstrating the utility<br />
of the MVV system for easily assessing and altering the aspirate/dispense parameters for creating optimized liquid<br />
delivery protocols will be discussed.<br />
2:00 pm Thursday, February 5 Emerging Technologies – Performance Metrics Room A1<br />
Toshiyuki Shiina<br />
Los Alamos National Laboratory<br />
P.O. Box 1663 MS J580<br />
Los Alamos, New Mexico 87545<br />
tshiina@lanl.gov<br />
Co-Author(s)<br />
Torsten Staab<br />
Derek Miller<br />
Improving Sample Analysis Throughput and Quality With a C#.NET-based, Real-Time QC<br />
Decision Support System<br />
In this talk we present the current status and the future plan of an integrated, software-based quality control<br />
system designed to significantly improve the sample analysis throughput and quality of Beryllium analysis<br />
laboratories throughout the U.S. Department of Energy complex. Originally this system was developed for the<br />
Perkin Elmer 4300DV Optical Emission Spectrometer, which is usually combined with an auto-sampler capable<br />
of more than 100 samples per run. The current implementation of the device control software has no feedback<br />
loop; hence requires constant manual monitoring by lab personnel. Our new control system is designed to enable<br />
automatic data analyses in real-time in order to reduce operational costs and human error. To achieve these goals,<br />
we have developed a rule-based expert system in the C#.NET programming language and ADO.NET (ActiveX<br />
Data Object) that continuously extracts and analyzes data from the instrument’s result database as the data is<br />
being generated. Using our customer-specified rule base, the system is capable of detecting abnormal operating<br />
situations fully autonomously in real-time. This also enables the system to perform on-the-fly quality control and<br />
automatic event notification of lab personnel via e-mail and/or pager.<br />
PODIUM ABSTRACTS
2:30 pm Thursday, February 5 Emerging Technologies – Performance Metrics Room A1<br />
Paul Taylor<br />
Boehringer Ingelheim<br />
175 Briar Ridge Road<br />
Ridgefield, Connecticut 06877<br />
ptaylor2@rdg.boehringer-ingelheim.com<br />
Application of Integrated Statistical Design and Analysis to Automated Assay<br />
Optimization (AAO)<br />
142<br />
Co-Author(s)<br />
Jeff Yingling, Mohammed Kashem,<br />
Richard Nelson, Carol Homon<br />
In the last several years high throughput screening has become a sophisticated automated environment capable<br />
of processing tens of thousands of samples per day. In this context bottlenecks have shifted from screening<br />
production to other areas including assay development. Statistically designed experiments have been in use since<br />
the pioneering work of Sir Ronald A. Fisher in the 1920s. Their application to the optimization of biological assays<br />
has, however, been limited due to the sheer number of factors and the complexity of their responses. Intricate<br />
interactions and non-linear behaviors are a common hallmark of biological systems and this has suggested<br />
multivariate experimental design and analysis as a useful development tool. It is only within the last several years<br />
that the capability of translating randomized statistical designs into robotic protocols has become feasible. As<br />
such, aut<strong>omation</strong> has opened the door to novel means of experimentation that would be impossible to carry<br />
out manually due to time limitations and design complexity. Initially, simpler fractional factorial experiments were<br />
reported as being effective and this has led to the present aim of incorporating response surface design and<br />
predictive modeling. The attractiveness of this approach is that it allows statistical analyses to identify optima that<br />
are distinct from experimental test conditions.<br />
3:00 pm Thursday, February 5 Emerging Technologies – Performance Metrics Room A1<br />
Peter Grandsard<br />
Amgen, Inc.<br />
One Amgen Drive<br />
Thousand Oaks, California 91320<br />
peterg@amgen.com<br />
Building Confidence in Aut<strong>omation</strong><br />
Co-Author(s)<br />
John Alexander<br />
Henry Schultz<br />
To get information on equipment performance levels, several Amgen’s aut<strong>omation</strong> users perform standardized<br />
tests at regular intervals with results instantaneously recovered, analyzed statistically and stored in a database.<br />
When these tests reveal problems or nonconforming performance, users and aut<strong>omation</strong> specialists are alerted<br />
by e-mail and the appropriate repairs or modifications take place. This program is one way of generating and<br />
maintaining confidence in automated systems. Other methodologies for generating that necessary level of<br />
confidence rely on well-defined initial validations, operational procedures, roles and responsibilities, or make use of<br />
the corporate web portal. They will be described using a small set of case studies in the areas of small molecule<br />
storage and retrieval, high throughput screening, and pharmaceutics.
NOTES<br />
143
NOTES<br />
144
POSTER ABSTRACTS<br />
TP – Tuesday Posters should be set up on Tuesday 10:00 – 10:30 am and removed by 6:30 pm on Tuesday.<br />
The presenting author must be present 1:30 – 3:00 pm on Tuesday.<br />
WP – Wednesday Posters should be set up on Wednesday 10:00 – 10:30 am and removed by 6:30 pm on<br />
Wednesday. The presenting author must be present 2:00 – 3:30 pm on Wednesday.<br />
145<br />
POSTER ABSTRACTS
TP001<br />
Thor Anders Aarhaug<br />
SINTEF<br />
Materials Technology<br />
Sem Sælandsvei 12<br />
TrondheimNO-7465 Norway<br />
taarhaug@ntnu.no<br />
Sintalyzer – A Fully Automated Analytical System for Fluoride Analysis<br />
146<br />
Co-Author(s)<br />
Kalman Nagy<br />
In the aluminum industry, fluorine analyses are interfered by the presence of aluminum, silicon and iron. Performing<br />
standard fluorine analyses using TISAB buffers will result in a negative analytical error due to complexation of<br />
fluoride. The Sintalyzer analytical system is a customized system for fluoride analysis in the aluminum industry. It<br />
is used exclusively in the Scandinavian countries, and has also been installed in Germany, India, and Slovakia. The<br />
system uses several buffers that, in addition to proper sample preparation, render the fluoride electrochemically<br />
measurable. For optimal analytical performance, a standard addition technique is used. To avoid a dilution of the<br />
original matrix, micro volumes of highly concentrated standard is used. Since the concentration-voltage relation is<br />
given by the Nernst equation, the addition volumes have to be calculated dynamically in order to ensure an equal<br />
distance between the responses from the fluoride electrode. Although there are alternatives to potentiometry for<br />
fluoride analysis in the aluminum industry, the investment cost of, for example an XRF, are 10 times higher and<br />
requires by far more maintenance. Also, the sensitivity of the ion-selective electrode yields a better analytical<br />
performance. The Sintalyzer analytical system utilizes a standard addition technique that, by changing the ionselective<br />
electrode, can be extended to several other ions. By applying a lead ion-selective electrode, sulphur<br />
dioxide can be determined by titration with lead nitrate. The system comes with complete procedures for the<br />
analysis of fluoride of all matrices that are common in aluminum industry. The system is accredited according to<br />
Norwegian Standard quality assurance of GLP.<br />
TP002<br />
Monica Adams<br />
Amersham Biosciences<br />
Development Integration/Genomics<br />
800 Centennial Avenue<br />
Piscataway, New Jersey 08854<br />
monica.adams@amersham.com<br />
The Aut<strong>omation</strong> of TempliPhi and GenomiPhi on the Tecan Genesis Freedom<br />
The Genesis Freedom Aut<strong>omation</strong> Workstation from Tecan offers you a simple, effective way to gain all the<br />
advantages of liquid handling and laboratory aut<strong>omation</strong>, which was used to automate Amersham Bioscience’s<br />
TempliPhi and GenomiPhi kits. The Tecan Gemini software system allows easy protocol set-up and operation<br />
of the Freedom workstation. Amersham Bioscience’s TempliPhi Amplification kit is a novel process to efficiently<br />
prepare DNA sequencing templates in 4 – 6 hours. Templates are prepared by rolling circle amplification using<br />
bacteriophage Phi29 DNA polymerase.The GenomiPhi kit utilizes Phi29 DNA polymerase enzyme to exponentially<br />
amplify single and double stranded DNA by strand displacement amplification. The Tecan Freedom, with an eightchannel<br />
liquid handling arm, is capable of generating multiple TempliPhi or GenomiPhi 96-well reaction plates in<br />
more than half the time of manual pipetting. TempliPhi and GenomiPhi can be easily automated to work with liquid<br />
handling systems.
TP003<br />
Edward Alderman<br />
Zymark Corporation<br />
Drug Discovery Consulting<br />
Zymark Center - 68 Elm Street<br />
Hopkinton, Massachusetts 01748<br />
ed.alderman@zymark.com<br />
Co-Author(s)<br />
Geoffrey N. Grove, Zymark Corporation<br />
Mei Cong and Zhong Zhong, Cell & Molecular Technologies<br />
Chris Cowan, Promega<br />
Casey Laris, Q3DM<br />
Aut<strong>omation</strong> of Apoptosis and Reporter-Gene Assays Using Division-Arrested<br />
NFkB HEK293 Cells<br />
Due to the high failure rate of NCE’s in animal trials, researchers are increasingly choosing to run assays in more<br />
physiologically relevant systems. This has given rise to an increasing demand for cell-based assays earlier in drug<br />
discovery and development work. We demonstrate that a cell-culture facility is not needed to perform automated<br />
cell-based assays. Using the Staccato Sciclone Cell Station-Assay (an automated cell-based assay system) and<br />
division arrested HEK293 cells provided by CMT (Cell & Molecular Technologies), we present data demonstrating<br />
the equivalency of division-arrested cells to normal cells when assayed using Promega’s Apo-ONE Homogenous<br />
Caspase-3/7 and Steady-Glo ® Luciferase Assay Systems.<br />
TP004<br />
Chris Barbagallo<br />
Millipore Corporation<br />
Research & Development<br />
17 Cherry Hill Drive<br />
Danvers, Massachusetts 01923<br />
chris_barbagallo@millipore.com<br />
Automating Aqueous Compound Solubility Screening<br />
147<br />
Co-Author(s)<br />
Greg Kazan<br />
Libby Kellard<br />
Jason Blodgett<br />
Alan Weiss<br />
Compound solubility characterization in the early stages of the drug discovery process is becoming an essential<br />
tool prior to performing any biological testing. The main reason for this being that low solubility can lead to<br />
unreliable results during in vitro studies, such as the generation of false positives in these bioassays. This wastes<br />
valuable time and resources, which can add significant cost to drug research projects. In addition to these<br />
factors, the standard shake-flask method now used to evaluate drug solubility is inherently low throughput and<br />
labor intensive. Millipore has developed a 96-well, filter-based method for both semi-quantitative and quantitative<br />
solubility determinations. We describe the use of these protocols on several liquid handling platforms to show the<br />
ease and robustness of full aut<strong>omation</strong> of this process. Results shown correlate well with values obtained using<br />
the shake-flask method. Depending on calibration and replicate number, this procedure is capable of generating<br />
results for hundreds of samples per day.<br />
POSTER ABSTRACTS
TP005<br />
Alex Berhitu<br />
Spark Holland, Inc.<br />
666 Plainsboro Road, Suite 1336<br />
Plainsboro, New Jersey 08536<br />
alex.berhitu@sparkholland.com<br />
Denaturing Solid-Phase Extraction for Reduced Protein Interference in<br />
Bioanalytical SPE-LC-MS<br />
148<br />
Co-Author(s)<br />
Emile Koster<br />
Peter Ringeling<br />
Bert Ooms<br />
Solid-Phase Extraction (SPE) coupled on-line to LC-MS can provide fully automated assays with 100%<br />
recovery and high precision. However, many matrix compounds (e.g., proteins) are usually also trapped and can<br />
subsequently co-elute with the drugs. Although most co-eluting proteins do not interfere with detection, they can<br />
reduce the column lifetime or cause ionization suppression. Even though more than 95% of serum proteins are<br />
removed by reversed-phase on-line SPE and only a part of the trapped proteins elute into the analytical system,<br />
suppression of the MS response and reduced LC-column lifetime are sometimes reported. We have investigated<br />
the effect of denaturing SPE conditions on the amount of eluting proteins using direct UV monitoring of SPE clean<br />
up. To that end serum is loaded onto a HySphere C18 cartridge with acidified water and a wash step is performed<br />
under denaturing conditions such as high zinc sulfate concentration, high and low pH and high temperature.<br />
Results show that optimized denaturing wash conditions reduces the amount of co-eluting proteins significantly,<br />
which means longer column life time, less pollution of MS interface and reduced risk of ionization suppression.<br />
Compared to traditional off-line deproteinization (including centrifugation) followed by SPE, denaturing SPE is<br />
not only fully automated but it also gives cleaner extracts. Moreover, denaturing SPE reduces the risk of coprecipitation<br />
of drug and protein as separation of both occurs during the first SPE step.<br />
TP006<br />
Emily Berlin<br />
Pall Life Sciences<br />
600 South Wagner Road<br />
Ann Arbor, Michigan 48103<br />
emily_berlin@pall.com<br />
Co-Author(s)<br />
Michael L. Metzker, Luke Mast, Geoffrey Okwuonu and Toni Garner,<br />
Pall Life Sciences<br />
Kamran Usmani and Richard A. Gibbs, Baylor College of Medicine<br />
Meeting the Increasing Challenges of Speed, Performance, and Quality While Reducing<br />
Costs in Whole Genome Sequencing<br />
Following the completion of the human genome sequence, the BCM-HGSC in collaboration with its respective<br />
partners has recently released the final draft assemblies of the rat, Drosophila pseudoobscura, and honey bee<br />
genomes. Current efforts of draft quality sequence and subsequent assembly of the sea urchin, Rhesus macaque,<br />
and bovine genomes present new challenges in speed and performance while maintaining high quality and<br />
reduced costs. Additionally, tracking of species-specific projects and associated BAC clones to support our<br />
blended whole genome shot-gun strategy for Atlas assembly of whole genomes is critical for efficient project<br />
management. Our recent innovations in aut<strong>omation</strong> technology in both production and instrument loading groups,<br />
and the development of the 192-well plasmid purification platform have been instrumental in meeting these<br />
challenges. For example, the 192-well plate format increases throughput 2-fold at reduced materials and reagent<br />
cost while requiring the same number of research personnel and equipment (i.e., prep robots, storage freezers,<br />
and shakers). Single plate-to-plate transfers for paired-end DNA sequence reaction set-up ensures robust tracking<br />
efficiency of projects through our production pipeline. The current status of different whole genome projects and<br />
the underlying production strategies to ensure the highest quality draft sequence products will be presented.
TP007<br />
Christopher Bernard<br />
Bristol-Meyers Squibb Co.<br />
Discovery Technologies<br />
5 Research Parkway<br />
Wallingford, Connecticut 06492<br />
chris.bernard@bms.com<br />
149<br />
Co-Author(s)<br />
Moneesh Chatterjee, Andrew Bullen, James Myslik,<br />
William Monahan, Jeffrey Guss, Christian Strom,<br />
Jay Stevenson, Alastair Binnie<br />
A Microtube Rack Decapper/Sorter for use in the Automated Preparation of Compounds for<br />
High Throughput Screening<br />
A key requirement for using large compound collections in High Throughput Screening (HTS) is making<br />
compounds available in formats appropriate for screening. In most instances, part of the formatting process is<br />
dependent on converting compounds from a 96-well microtube format to more densely packed plate formats<br />
(i.e., 384-well or 1536-well). The first step after retrieval from the compound collection is to remove caps from<br />
individually sealed microtubes, and sort the microtube racks into specific groupings. Typically, the process of<br />
decapping and sorting is a manual procedure with inherent ergonomic stress, that also introduces the potential for<br />
racks to be grouped incorrectly. The lack of a commercially available system that was cost-effective and capable<br />
of automatically removing EVA caps from Micronic microtubes led us to create a custom system for decapping<br />
microtube racks. To address rack handling and sorting, a custom-made decapping device was integrated with<br />
commercially available stackers (VStack, Velocity11), a random access carousel (CRS), and a Vertical Array Loader<br />
(CRS) into a linear rail-based system. The overall system is run by a custom instrument control and scheduling<br />
application developed in LabVIEW (National Instruments). Additionally, sophisticated error trapping and inspection<br />
capabilities are included in this system to further enhance overall process reliability and to aid in recovery from<br />
system errors. The implementation of this custom system to decap and sort microtube racks in an automated<br />
production environment will be presented.<br />
POSTER ABSTRACTS
TP009<br />
Stefan Betz<br />
Kendro Laboratory Products GmbH<br />
Robert Bosch Str. 1<br />
Langenselbold, 63505 Germany<br />
stefan.betz@kendro.spx.com<br />
Production & Storage of High Density Chemical Microarrays<br />
150<br />
Co-Author(s)<br />
Michael Frank, Graffinity Pharmaceuticals AG<br />
Chemical microarrays are applied by Graffinity as part of a proprietary drug discovery platform which combines<br />
chemistry and biology with physics, information technology, and microsystem technology. As a result characteristic<br />
fingerprints of examined proteins provide detailed chemo-biological information for the subsequent “RAISE”<br />
process (Rapid Affinity Instructed Structure Evolution). In this process, small organic molecules are developed in an<br />
evolutionary way directed by their interactions with target proteins. Gold-coated glass chips are used as carriers<br />
for Graffinity’s chemical microarrays. The arrays have standard microtiter plate footprint with up to 9216 spots or –<br />
the latest generation – microscopy-slide size with up to 9600 spots. The gold layer is coated with a self assembling<br />
monolayer (SAM) of synthetic chemicals. The surface preparation was developed by Graffinity to enable the<br />
immobilization of chemical compounds while preventing the nonspecific binding of proteins. The transfer of<br />
chemical compounds onto the surface of the microarrays is achieved utilizing a spotting procedure. The spotting<br />
of the substances is reliable and precise, placing compounds onto the microarrays in a custom-built automated<br />
environment to ensure both quality and reproducibility at high throughput. For mixed storage of MTP-size as well<br />
as slide-size microarrays a modified Kendro Cytomat 6002 is used. The storage hotel enables space-saving inert<br />
and cooled storage of up to 700 MTP-size or 2800 slide-size microarrays per unit.<br />
TP010<br />
Laurent Bialy<br />
University of Southampton<br />
Chemistry<br />
Highfield<br />
Southampton SO17 1BJ United Kingdom<br />
laurent.bialy@gmx.de<br />
PNA-encoded Peptide Libraries – A New Tool in High Throughput Screening<br />
Co-Author(s)<br />
Mark Bradley<br />
Peptide nucleic acids were described by Nielsen as stable DNA analogues. We want to use PNA-DNA<br />
hybridisation to “arrange” in defined locations on a DNA chip each and every specific member of a split-and-mix<br />
library and then interrogate the library while attached to the array. A split-and-mix library of peptides covalently<br />
attached to an encoding PNA arm was synthesized and after cleavage from the resin was allowed to hybridize<br />
to a DNA-chip. This DNA chip can be used to screen the peptide library for reversible binding of various receptor<br />
molecules which are fluorescently labeled. Small, organic molecules which behave as selective binders for<br />
peptides could be used as therapeutic agents. Through binding peptidic hormones or growth factors or inhibiting<br />
protein-protein interactions which are crucial for biological signaling, these receptors could become a valuable<br />
tool in molecular biology or even become lead compounds for anti-cancer drugs. Our techonology is superior to<br />
classical on-bead-screening techniques because we can do our assays in homogeneous solution and hybridize<br />
to the chip afterwards, thereby avoiding artefacts of heterogeneous assays. Furthermore, we could also reuse<br />
our chips for more than one assay by simply washing off reversibly binding molecules. In conclusion, this new<br />
screening technology should be a valuable alternative to other techniques like on-bead-assays in the field of high<br />
throughput screening technology.
TP011<br />
Annegret Boge<br />
Molecular Devices<br />
Biochemistry<br />
1311 Orleans Drive<br />
Sunnyvale, California 94089<br />
annegret_boge@moldev.com<br />
Adaptation of a High Sensitivity 384-Well Solid Phase Assay Platform to 1536-Well:<br />
CatchPoint for Cyclic Nucleotides and Tyrosine Kinase Activity<br />
151<br />
Co-Author(s)<br />
Jonathan Petersen<br />
Jeannie Nguyen<br />
Gayle Teixeira<br />
Richard Sportsman<br />
The Molecular Devices Catchpoint platform is a very sensitive solid phase based assay system for assaying for<br />
cAMP, cGMP and tyrosine kinase activity. In order to make such a solid phase system amendable to ultra high<br />
HTS (even if it includes only one wash step) we have adapted it from 384-well plates to 1536-well plates. This<br />
adaptation was possible by the concurrent development of a new Washer/Dispenser System (AquaMax DW4) by<br />
Molecular Devices. We compare several performance parameters of the different assays between 384-well plates<br />
and 1536-well plates including sensitivity and variability.<br />
TP012<br />
Wayne Bowen<br />
TTP LabTech<br />
Melbourn Science Park<br />
Melbourn, Herts SG8 6EE United Kingdom<br />
wayne.bowen@ttplabtech.com<br />
A Homogeneous Assay for P-glycoprotein Inhibition Using the Acumen Explorer<br />
Co-Author(s)<br />
Tristan Cope<br />
Matthew Cook<br />
Interindividual differences in drug absorption and disposition are an important cause of drug therapy failures.<br />
Evidence is increasing that active drug transport across cellular membranes is an important process involved in<br />
drug absorption and disposition. P-glycoprotein (Pgp) is an ATP dependant transmembrane protein, that actively<br />
transports molecules out of cells, including drugs such as digoxin and HIV protease inhibitors. Screening for<br />
inhibitors of Pgp activity at an early stage can thus highlight potential drug-drug interactions. Pgp activity was<br />
measured using the fluorescence due to calcein accumulation in the cytosol, produced by the action of cellular<br />
esterases on the non-fluorescent Pgp substrate, calcein-AM. Cell viability was assessed simultaneously through<br />
addition of the nuclear stain Propidium Iodide. Cellular fluorescence was measured using an Acumen Explorer<br />
laser scanning fluorescence microplate cytometer. This is a non-confocal system, permitting area-based scanning<br />
of fluorescent objects located on the bottom of micro-titre plate wells. The Acumen Explorer does not need to<br />
re-focus between wells, conferring high scan speed on any SBS standard plate, including 1536-well plates. It<br />
offers a 488 nm excitation source and up to 4 channels of data collection. The effect of verapamil, ketoconazole<br />
and rifampicin on Pgp activity was measured in HepG2 cells. All the compounds produced an increase in cellular<br />
fluorescence relative to untreated cells. The rank order of potency was verapamil = ketoconazole > rifampicin.<br />
Limited cytotoxicity was observed with ketoconazole at high concentrations (> 30 µM). These data demonstrate<br />
the utility of the Acumen Explorer in primary, mulitplexed ADMET screening.<br />
POSTER ABSTRACTS
TP013<br />
Christine Brideau<br />
Merck Frosst Centre for Therapeutic Research<br />
Biochemistry & Molecular Biology<br />
16711 TransCanada Highway<br />
Kirkland, Quebec H9H 3L1 Canada<br />
christine_brideau@merck.com<br />
Co-Author(s)<br />
Louis Jacques Fortin, Shiraz Adam and Jerry Ferentinos,<br />
Merck Frosst Centre for Therapeutic Research<br />
Joel Hunter, RTS Enabling Technology<br />
Julio Maher, RTS Life Science International<br />
SOS – A Sample Ordering System to Deliver “Assay-Ready” Compound Plates for Screening<br />
Many bottlenecks in drug discovery have been addressed with the advent of new assay and instrument<br />
technologies. However, storing and processing chemical compounds for screening remains a challenge for many<br />
drug discovery laboratories. Automated storage and retrieval systems are commercially available for medium to<br />
large collections of chemical samples. However, these samples are usually stored at a central site and are not<br />
readily accessible to satellite research labs. Drug discovery relies on the rapid testing of new chemical compounds<br />
in relevant biological assays. Therefore, newly synthesized compounds must be readily available in various<br />
formats to biologists performing screening assays. Until recently, our compounds were distributed in screw cap<br />
vials to assayists who would then manually transfer and dilute each sample in an ‘assay-ready’ compound plate<br />
for screening. The vials would then be managed by the individuals in an ad hoc manner. To relieve the assayist<br />
from searching for compounds and preparing their own ‘assay-ready’ compound plates, a newly customized<br />
compound dispensing system and software application was implemented at our research facility that eliminates<br />
these bottlenecks. The system stores and retrieves compounds in 1mL-minitubes or microtiter plates, facilitates<br />
compound searching by identifier or structure, orders compounds at varying concentrations in specified wells on<br />
96- or 384-well plates, requests the addition of controls (vehicle or reference compounds), etc. The orders are<br />
automatically processed and delivered to the assayist the following day for screening. An overview of our system<br />
will demonstrate that we minimize compound waste and ensure compound integrity and availability.<br />
TP014<br />
Jimmy Bruner<br />
GlaxoSmithKline<br />
High Throughput Biology<br />
5 Moore Drive<br />
Durham, North Carolina 27709<br />
jimmy.j.bruner@gsk.com<br />
A Novel System for Automated RNA Isolation<br />
“Increasing Throughput Without Increasing Footprint”<br />
152<br />
Co-Author(s)<br />
Bryan Laffite, David Murray, Ginger Smith,<br />
Jim Liacos, Amy Siu, Cathy Finlay<br />
The High Throughput Biology department at GlaxoSmithKline is developing in vitro models to better predict<br />
the efficacy of compounds in the clinic. The development and progression of disease is often associated with<br />
characteristic changes in gene expression.These “transcriptional profliles” (mRNA gene expression patterns<br />
associated with a given disease) can be used as biomarkers to monitor disease states. Therefore, we are<br />
utilizing transcriptional profiling as a way to understand diseases and the effects of pharmaceuticals on those<br />
diseases.Transcriptional profiling has numerous advantages over other techniques including highly sensitive<br />
and highly quantitative assays, simple assay development, and accessibility of nearly the entire genome to<br />
transcriptional readouts. Our transcriptional profiling strategy involves isolation of total RNA from samples followed<br />
by real-time quantiative PCR assays (Taqman assays).Current automated systems allow for the isolation of total<br />
RNA from one 96-well plate at a time on a Biomek FX taking approximately one hour per plate. The Aut<strong>omation</strong><br />
team recently developed an automated system to increase throughput and reduce reagent waste for 96-well RNA<br />
isolation extractions. This system can isolate RNA from 10 96-well plates in approximately four hours. An additional<br />
goal was to create a system on one stand-alone Biomek FX. Beyond simultaneous processing of plates and<br />
custom deck lay out we designed and co-developed with Beckman a nested-tip design that allows the stacking of<br />
five P20 tip boxes on one alp location. This poster describes the nested tip functions, robot functions, and assay<br />
performance of a higher throughput automated RNA isolation workstation.
TP015<br />
Jesse Campbell<br />
Applied Molecular Evolution<br />
3520 Dunhill Street<br />
San Diego, California 92121<br />
jcampbell@amevolution.com<br />
153<br />
Co-Author(s)<br />
Michael J. Cohen, Andrew Korytko,<br />
Barbara Swanson, Alain P. Vasserot<br />
Development of a Data-driven, Integrated Aut<strong>omation</strong> Platform for High Throughput<br />
Expression and Screening of Protein Engineering Libraries<br />
Protein engineering can drastically impact the efficacy and safety profile of therapeutic proteins and expand their<br />
clinical utility. The systematic exploration of beneficial amino acid changes in a protein requires the creation,<br />
expression, and screening of mutational libraries and the use of a wide range of procedures and assays. In<br />
order to facilitate the rapid evaluation of these libraries, we modified a Biomek FX-based system to create an<br />
integrated aut<strong>omation</strong> platform that enables robotic handling of engineering projects with widely different goals<br />
and requirements. This system is now capable of automated colony picking, plasmid preparation, mammalian<br />
cell transfection, quantification and robotic execution of a variety of project-specific screening assays. Integrated<br />
analysis software allows data-driven normalization of expression levels and sample re-arraying as well as the<br />
selection of primary leads based upon real-time investigator input. The heavy use of custom-designed hardware<br />
and software solutions was necessary to design a platform that considerably expands the utility of the original core<br />
system by maximizing unattended overnight usage and by accommodating a wide variety of libraries, expression<br />
systems, and screening assays.<br />
TP016<br />
Julie Chang<br />
MDL Information Systems<br />
14600 Catalina Street<br />
San Leandro, California 94577<br />
jchang@mdl.com<br />
An Inventory Management Solution From Registration to Assays<br />
The compound management group is often described as the hub of activity that manages the formulation,<br />
storage, distribution, and job tracking of requests for plates to various groups that input compounds as well as<br />
request compounds for testing. MDL will present an information management solution that will accommodate the<br />
workflows of chemists as well as biologists in the new MDL Plate Manager.<br />
POSTER ABSTRACTS
TP017<br />
Madhu Prakash Chatrathi<br />
New Mexico State University<br />
Chemistry & Biochemistry<br />
MSC 3C; North Horseshoe Drive<br />
Las Cruces, New Mexico 88001<br />
madhupra@hotmail.com<br />
Co-Author(s)<br />
Joseph Wang, Alexander Muck, Scott Spillman, Gautham<br />
Sridharan and Michael Jacobs, New Mexico State University<br />
Michael Schonning, Institute of Thin Films and Interfaces,<br />
Jülich, Germany<br />
Rapid Fabrication of Poly(methylmethacrylate) Microfluidic Chips by Atmospheric Molding<br />
Microfluidic devices are finding numerous applications in analytical chemistry. Most early reports on miniaturized<br />
analytical systems have relied on glass or silicon substrates. However, the cost of producing glass microchips<br />
and the ease of fabrication has driven researchers and producers to seek for alternative materials. Recent efforts<br />
have thus led to increasing use of polymeric materials [poly(dimethylsiloxane) (PDMS), poly(methylmethacrylate)<br />
(PMMA) or polycarbonate (PC)] in the preparation of chip-based devices. PMMA has been particularly useful for<br />
analytical microsystems, owing to several advantages, including high chemical and mechanical stability and good<br />
support of the electroosmotic flow. A greatly simplified method for fabricating PMMA separation microchips is<br />
introduced. The new method of fabrication relies on UV initiated polymerization of the monomer solution in an<br />
open mold under ambient pressure. Silicon microstructures are transferred onto the polymer substrate by molding<br />
a methylmethacrylate solution in a sandwich (silicon-master/Teflon-spacer/glass-plate) mold. The chips are<br />
subsequently assembled by thermal sealing of the channel and cover plates. The new approach brings significant<br />
simplification of the process of fabricating PMMA devices and should lead to a widespread low-cost production<br />
of high-quality separation microchips and obviates the need for specialized replication equipment and reduces<br />
the complexity of prototyping and manufacturing. While the new approach is demonstrated on PMMA microchips,<br />
it could be applied to other materials that undergo light-initiated polymerization. Ongoing experiments in our<br />
laboratory are focused on tailoring the electroosmotic flow characteristics by changing the chemical properties of<br />
the bulk microchip material via judicious choice of the monomer material.<br />
TP018<br />
Melissa Cheu<br />
Genentech, Inc.<br />
BioAnalytical Assays<br />
1 DNA Way, Mailstop 86B<br />
South San Francisco, California 94080<br />
melissa@gene.com<br />
Validation of the Packard Multiprobe HT for Dilution of Biological Matrix Samples<br />
154<br />
Co-Author(s)<br />
Brent T. Nakagiri<br />
Riddhi Patel<br />
Patricia Y. Siguenza<br />
The BioAnalytical Assays Department at Genentech, Inc. performs drug level measurement and antibody to<br />
product testing in support of pre-clinical and clinical studies. To support drug level measurements, biological matrix<br />
samples are diluted in various diluents to fall within the standard curve ranges of 96-well plate based ELISAs.<br />
Since the concentration of drug varies in the samples being tested, some samples may need to be diluted more<br />
than others to fall within the standard curve range. The need for a dilution instrument that could simultaneously<br />
dilute a set of samples to multiple endpoints led to the selection of the Packard Multiprobe HT dilutor, a robotic<br />
liquid handling system capable of delivering up to eight different liquid volumes simultaneously through eight<br />
independently controlled syringes. Since BioAnalytical Assays runs samples in a regulated environment, validation<br />
of the Packard Multiprobe HT was performed before the instrument was put into use for dilution of samples.<br />
Validation testing included accuracy and precision checks of the instrument, error generation testing, and recovery<br />
of reference material diluted into plasma, serum, and buffer.
TP019<br />
Robin Clark<br />
deCODE genetics<br />
BioStructures Group<br />
7869 NE Day Road W.<br />
Bainbridge Island, Washington 98110<br />
rclark@decode.com<br />
Protein Maker: An Automated System for Protein Purification<br />
Co-Author(s)<br />
Alexandrina Muntianu, Hans-Thomas Richter, Denise Conner,<br />
Lawrence Chun, Lance Stewart<br />
The Protein Maker is an automated system for parallel liquid chromatography at medium scale (1-50 mg of<br />
protein). Protein solutions, wash buffers and elution buffers are delivered under positive pressure to up to 24<br />
columns by automated syringe pumps. Thus Protein Maker is analogous to an FPLC system that can run 24<br />
columns at a time. The 24 syringe pumps are connected to 8-way valves, with each pump and valve controlled<br />
independently through the software. A gantry with XYZ directional control carries two 24-port manifolds: one for<br />
sample loading and one for columns. Standard pre-packed 1 to 10 ml columns are mounted on the gantry with<br />
flow rates adjustable from 0.25 to 290 ml/min. Column fractions are delivered into 24-well block plates at any of 23<br />
deck locations. Protein Maker can be used to purify up to 24 different proteins in parallel on duplicate columns, or<br />
to test multiple purification strategies on one or a few proteins. Application results will be presented.<br />
TP020<br />
Matthew Cook<br />
TTP LabTech<br />
Melbourn Science Park<br />
Melbourn, Herts SG8 6EE United Kingdom<br />
matthew.cook@ttplabtech.com<br />
155<br />
Co-Author(s)<br />
Olivier Dery, Gwyneth Olson, Annick Le Gall and<br />
Francine Fang, TTP LabTech<br />
Pierre Turpin, BD Bioscience Clontech<br />
BD Biosciences Clontech ZsProSensor-1, a Fluorescent Protein-based Proteasome Sensor<br />
Assay: Evaluation Using the Acumen Explorer<br />
Protein degradation by the proteasome is at the core of many pathological processes such as inflammation,<br />
autoimmunity, neurodegenerative diseases, and cancer. This has motivated efforts to identify compounds that<br />
modulate the activity of the proteasome. Assays to monitor this activity in live cells could boost these efforts by<br />
bypassing heavy biochemical manipulations. In this study, a proteasome targeting sequence was used to direct<br />
the reef coral fluorescent protein ZsGreen to degradation by the proteasome. The chimaeric fluorescent protein<br />
ZsProSensor-1 is constitutively degraded by the proteasome in stably transfected HEK 293 cells and accumulates<br />
under conditions that alter the activity of the proteasome. Because of its high sensitivity, this live cell assay allows<br />
monitoring of the activity of the proteasome by microscopes, flow cytometers and standard 96-well plate readers.<br />
Using the Acumen Explorer the inhibitory activities of four different inhibitors of the proteasome were detected<br />
at discrete time points using ZsProSensor-1-expressing cells. Propidium Iodide (PI) was used to monitor the<br />
cytotoxicity of these compounds. These data demonstrate the complimentary nature of BD Biosciences Clontech’s<br />
Novel Fluorescent Protein (NFP) based assays and the Acumen Explorer fluorescent detection system for the<br />
potential identification of compounds that modulate the activity of the proteasome as well as their cytotoxicity and<br />
other cellular effects.<br />
POSTER ABSTRACTS
TP021<br />
Cristopher Cowan<br />
Promega Corporation<br />
Applications<br />
2800 Woods Hollow Road<br />
Madison, Wisconsin 53711<br />
ccowan@promega.com<br />
156<br />
Co-Author(s)<br />
James Batchelor, Zymark Corporation<br />
Automated Isolation of Nucleic Acids on the Zymark SciClone ALH 3000 Using Promega’s SV<br />
96 Nucleic Acid Isolation Chemistries<br />
Isolation of nucleic acids is central to all manipulations used in molecular biology. Promega offers a variety of<br />
SV 96 Nucleic Acid Isolation Systems which provide a coherent set of vacuum based isolation protocols for<br />
purification of genomic DNA from tissues and tissue culture cells, total RNA from tissues and tissue culture cells,<br />
plasmids from bacterial cell cultures and PCR clean-up from PCR reactions. In collaboration with Zymark we<br />
have developed methods for the purification of all of these nucleic acid types using Promega’s SV 96 Nucleic<br />
Acid Isolation Systems on the Zymark SciClone ALH 3000 liquid handling workstation. The SV 96 Nucleic Acid<br />
Isolation Systems use filter plates for binding nucleic acids. Instead of using vacuum filtration for binding and<br />
washing steps, the Zymark SciClone ALH 3000 liquid handling workstation uses a positive pressure system to<br />
push reagents through the filter plates. Once bound, the nucleic acids are washed and the highly purified nucleic<br />
acid products are eluted in water. Once isolated, the samples can be used directly for downstream PCR, RT-PCR,<br />
quantitative PCR, sequencing and other molecular biological reactions. We demonstrate the automated isolation of<br />
a variety of nucleic acid types (genomic DNA, total RNA, plasmid, PCR product) from tissues, tissue cell cultures,<br />
bacterial cultures, and PCR reactions. Performance of the isolated nucleic acids in PCR, RT-PCR, and sequencing<br />
is demonstrated, with no detectable cross contamination.<br />
TP022<br />
Carole Crittenden<br />
Molecular Devices Corporation<br />
1311 Orleans Drive<br />
Sunnyvale, California 94089<br />
Carole_Crittenden@moldev.com<br />
Demonstration of Mitochondrial Aequorin Luminescent Signal Measurement by FLIPR3<br />
Co-Author(s)<br />
Jennifer McKie<br />
Yan Zhang<br />
The FLIPR3 ® is a cell-based HTS system that measures intracellular fluorescence assays such as calcium flux<br />
and membrane potential. Using a sensitive camera, the high throughput capabilities of the FLIPR3 system are<br />
expanded to include measurement of aequorin luminescent signal in cell-based G Protein Coupled Receptor<br />
Assays for drug discovery.FLIPR3 simultaneously reads all wells throughout the reagent addition process.<br />
The ability to read immediately after dispensing enables aequorin that yield flash luminescence. In this study,<br />
we demonstrate luminescence measurement capabilities for FLIPR3 and evaluate cell based luminescence<br />
applications for drug discovery.
TP023<br />
Katherine Dains<br />
Adeline Scientific<br />
Research<br />
1534 Plaza Lane, Suite 119<br />
Burlingame, California 94010<br />
kdains@earthlink.net<br />
157<br />
Co-Author(s)<br />
Wensheng Chen<br />
Information and Data Management Software for Tracking Patient, Research, and Analysis<br />
Data in Small to Medium Clinical and Research Labs<br />
We have developed software for information and data management for use in the small- to medium-sized life<br />
science research labs. The system is user configurable, uncomplicated, and affordable. Most commercial data<br />
management software products available require sophisticated IT setup, in-house personnel or outside contractors<br />
to control and maintain the system. Our system will appeal to many laboratories that require flexibility and<br />
specificity to their laboratory needs without the complexity and cost of a large and expensive product. At the<br />
center of the system is a configuration tool that can be set up to handle various laboratory data management<br />
workflows such as recruitment and tracking of research study candidates and related genetic studies. This<br />
intelligence tool is made possible through a proprietary data model cartridge technology. After completing the<br />
configuration process, the user can choose to deploy the data system or to merge data with existing systems. The<br />
system is designed with three-tier architecture in the form of web applications thus allowing for easy upgrades and<br />
remote access. Along with the simple web-based architecture, user-configurable data loading tools are provided<br />
that will adapt to most data importing tasks. Information can be entered and tracked over long periods of time,<br />
as well as easily retrieved through the web-based reporting structure. The system is built to support all major<br />
database products. We will present details on the architecture, detailed design specifications, and test results from<br />
collaborating research labs.<br />
TP024<br />
Juan Jose Diaz-Mochon<br />
University of Southampton<br />
Chemistry<br />
Highfield Campus<br />
Southampton SO17 1BJ United Kingdom<br />
jjd29@soton.ac.uk<br />
Novel PNA-Peptoid Conjugates as Antisense Drugs<br />
Co-Author(s)<br />
Laurent Bialy, Boon-ek Yingyongnarongkul,<br />
Adam Belson, Mark Bradley<br />
Peptide Nucleic Acid (PNA) has been developed in the last decade as a new DNA mimic which hybridises<br />
complementary DNA or RNA sequences with higher affinity than its counterparts. PNA presents a 2aminoethylglycine<br />
backbone instead of the DNA sugar-phosphonate moiety. This uncharged backbone allows<br />
PNA to display a stronger hybridisation with DNA or RNA and to be easily synthesised. These features prompted<br />
investigators to develop PNA analogues as promising candidates for the antisense therapy strategies. Antisense<br />
drugs target genes that are active or over expressed in certain pathological processes thus disrupting the synthesis<br />
of disease-responsible proteins. However, the main drawback has been their lack of cellular permeability that<br />
has avoided their further development as efficient antisense drugs. A few promising PNA-conjugates have been<br />
reported but new models are necessary to improve cellular permeability and overall drug-like features. Herein a<br />
new type of PNA-conjugates with higher cellular permeability is introduced. These novel PNA oligomers are bound<br />
to peptoids that have been reported as successful transfection agents. A library of PNA analogues has been<br />
synthesised on solid phase using a novel deprotection strategy, and it has been tested on GFP-transfected cell<br />
cultures. Furthermore, this library will also be tested using a cell-based microchip assay.<br />
POSTER ABSTRACTS
TP025<br />
James Dixon<br />
North Carolina State University<br />
Department of Chemistry<br />
Dabney 413<br />
Raleigh, North Carolina 27695-8204<br />
jmdixon2@unity.ncsu.edu<br />
158<br />
Co-Author(s)<br />
Jonathan S. Lindsey<br />
Developing a Versatile Program and Database for use in Laboratory Science (PhotochemCAD)<br />
Laboratory researchers often face the problem of organizing and managing large quantities of experimental<br />
data, as well as comparing their data with literature data. In the photochemical sciences, the core data include<br />
the spectral properties of diverse substances. One wants to know the absorption and emission properties of a<br />
compound, have pointers to the literature, and to be able to perform calculations using such spectral data. We<br />
have developed a software package (PhotochemCAD) that includes a database of spectral data with literature<br />
references for 125 compounds of natural and synthetic origin. Features are available for performing diverse<br />
calculations of interest across the photochemical sciences, including Förster energy transfer, oscillator strength,<br />
multicomponent analysis, blackbody radiator, etc. The program (version 1, 1998) can be downloaded:<br />
http://www.kumc.edu/POL/PAPHome/Vol68/pap68sd1.html<br />
We have been working on developing version 2 of PhotochemCAD. The upgraded version includes features<br />
for spectral manipulation and display, addition of data to the spectral database, calculations related to energy<br />
transfer among pigments, and integrated help features. We also are working to incorporate spectra for additional<br />
compounds to the database. Other features have been incorporated to create a cleaner and more intuitive<br />
interface, and to make the downloading process seamless. This development effort has much in common with<br />
research aimed at creating versatile software programs that address the needs of experimentalists in laboratory<br />
settings. This presentation will provide an overview of the program and the solutions employed in upgrading to the<br />
new version.<br />
TP026<br />
Doug Drake<br />
IDBS<br />
2 Occam Court, Surrey Research Park<br />
Guildford GU2 7QB United Kingdom<br />
ddrake@id-bs.com<br />
Co-Author(s)<br />
Andrew Lemon<br />
Evgueni Kolossov<br />
In Silico Drug Profiling: QSAR Models as Frontline Weapons in the Fight to Find New Drug<br />
Candidates<br />
Traditionally, model building, prediction and virtual screening has been an expert-only field, limiting more general<br />
application of such techniques. Related information and knowledge transfer between therapeutic groups has<br />
been limited and therefore the value of such models has never been fully realized. We present a model building<br />
system to develop and refine QSAR models. Designed to support rapid creation of high quality QSAR models<br />
using a variety of algorithms, the system supports creation, validation, and annotation of models. The models<br />
have application in virtual screening and property prediction of compound libraries, complementing the skills and<br />
knowledge of research scientists in designing new candidates. This platform provides a systematic approach to<br />
drug design providing the ability to build an in silico drug profile for as many relevant, measurable parameter as<br />
required. The system promotes QSAR modeling as a front line tool to aid drug discovery scientists.
TP027<br />
Philipp Dreiss<br />
Fraunhofer IPA<br />
Department Cleanroom Manufacturing<br />
Nobelstr. 12<br />
Stuttgart 70569 Germany<br />
Dreiss@ipa.fhg.de<br />
Capability Management Framework for Clinical Equipment in Laboratory<br />
159<br />
Co-Author(s)<br />
R. Muckenhirn<br />
J. Dorner<br />
A. P. Kumar<br />
The Capability Management Framework (CMF) system provides predefined and configurable sets of developed<br />
software components for the examination and evaluation of analysis results based on system and process<br />
capability patterns during the analysis process. CMF takes into account all the analysis steps and the machine<br />
parameters, which are necessary for the analysis to recognize the non-trivial coherence among the system,<br />
process capabilities and the related parameters, which can be responsible for the origination of system, process<br />
capability deviations. The evaluation criteria, strategies and expert knowledge are provided by the knowledge<br />
based system. CMF as an object-oriented framework can be used for a rapid development and integration<br />
of capability management based services within a clinical environment for scheduling and dispatching. These<br />
services extend the current features of Laboratory Information Management Systems (LIMS). The following<br />
presentation will depict the associated development process and the corresponding integration of its components<br />
in the existing software architectures and services for how to setup communication. It includes the basic<br />
framework elements, services and its integration to the other already existing software architectures. The main<br />
topics are the basic software components and the underlying communication infrastructure and protocols that<br />
support during a rapid setup and configuration process. Further the concepts for service registration and discovery<br />
in the context of the clinical environment are pointed out. The concept is shown by an implementation based for<br />
several equipment types as used in semiconductor manufacturing and pharmaceutical industries.<br />
TP028<br />
Ping Du<br />
Black Mountain Scientific<br />
4 Fullerton Place<br />
Livingston, New Jersey 07039<br />
blackmountainscientific@yahoo.com<br />
SHOW – Sample Handling Operation Wizard<br />
Manual sample handling, such as moving samples or recording data visually, is a tedious and error prone<br />
process. Sample Handling Operation Wizard (SHOW) is a system developed to guide manual sample handling in<br />
laboratories. It comprises a sample tray mounted on a 15" flat-panel computer monitor. Up to four transparent<br />
micro titer plates and up to eight reagent vials may be placed on the sample tray. Images of the wells of the plates<br />
and vials are generated on the monitor and controlled by computer software. These images are directly aligned<br />
with the positions of the physical wells or vials. By highlighting the wells or vials involved in a sample handling<br />
step of a predefined protocol, manual operations can be performed with precise guidance from the system. As a<br />
result, the risk of locating a wrong sample or placing a sample at a wrong location can be minimized, and sample<br />
handling operations become more efficient and less stressful.<br />
POSTER ABSTRACTS
TP029<br />
Wayne Duncan<br />
Agilent Technologies<br />
5301 Stevens Creek Boulevard<br />
P.O. Box 58059<br />
Santa Clara, California 95052-8059<br />
wayne_duncan@agilent.com<br />
Tools for Improving Purification Throughput for New Drug Candidates<br />
160<br />
Co-Author(s)<br />
Douglas McIntyre<br />
Bringing new drugs to market quickly is a considerable challenge for modern drug discovery laboratories due<br />
to the tasks of identification and purification of huge nu<strong>mbers</strong> of samples. Dealing with such large nu<strong>mbers</strong> of<br />
diverse compounds requires a robust system that chemists can rely on and that provides flexibility, yet is not<br />
difficult to use. Depending on the phase of development, the success of the synthetic schemes employed, and<br />
the subsequent biological testing required, the purification system must be very adaptable to provide optimum<br />
results. At times compound recovery is the most important consideration, while at other times maximum purity of<br />
the fractions is most critical. Then there are times when it is best to compromise by maximizing both purity and<br />
recovery simultaneously. All of this needs to be as automated and high throughput as possible. The system used<br />
in this work includes capabilities such as active splitting, integrated delay sensing between detectors and fraction<br />
collectors, and mass spectral and UV monitoring were used to precisely set system parameters. A key element<br />
is the ability to help the user set appropriate slope and threshold parameters by working with a preview screen<br />
during purification set-up. It is also shown how fraction homogeneity calculations can often alleviate the need<br />
for re-analysis of fractions to obtain purity information. Adding to the productivity of the system is the use of a<br />
walk-up and browser software for easy sample submission and data review by non-experts in purification such as<br />
medicinal chemists.<br />
TP030<br />
Todd Edwards<br />
Protedyne<br />
Field Technical Specialist<br />
8070A South Lake Drive<br />
Dublin, California 94568<br />
todde@protedyne.com<br />
Automated Real Time Hit Picking, Retesting, and Reflux Testing<br />
Co-Author(s)<br />
Jason Quarles, Dave Wilson,<br />
Ralph K. Ito, Gregory A. Endress<br />
Advances in aut<strong>omation</strong> have enabled screening methods, such as enzymatic assays and ELISAs, to be performed<br />
in a high throughput fashion. Instruments can perform each of the steps more rapidly and with fewer errors<br />
than humans, however, in many cases, humans still perform the key steps of integrating the instruments and<br />
Laboratory Information Management Systems (LIMS). Typically, the screening process involves multiple steps for<br />
sample testing, manual data entry, and design of protocols to handle the positive samples and final processing<br />
of positives. Robotic arms and track systems can bridge the gap between instruments, however the lack of a<br />
data transfer standard in instruments and LIMS makes the integration process difficult and often impossible for<br />
the typical biological laboratory. Fully integrated systems such as Protedyne’s BioCube System combine liquid<br />
handling, LIMS, plate washing, and plate reading in a device that is controlled via a single user interface. As an<br />
example, we present the results of automated ELISA testing that include sample plate preparation, plate washing,<br />
plate reading, and automatic retesting of positive samples. By performing an automated initial screening of results,<br />
our system enables positive samples to be retested at higher stringency or sent to a technician for corroboration of<br />
results. The screening process then becomes a single protocol that starts with a sample set and results in a data<br />
set of tested and retested/reflux tested positives as well as rearrayed plates of positive samples that are ready for<br />
additional downstream processing.
TP031<br />
Alex Berhitu<br />
Spark Holland, Inc.<br />
666 Plainsboro Road, Suite 1336<br />
Plainsboro, New Jersey 08536<br />
alex.berhitu@sparkholland.com<br />
161<br />
Co-Author(s)<br />
Steven Eendhuizen, Emile Koster,<br />
Martijn Hilhorst, Bert Ooms<br />
XLC-MS: Sample Extraction and LC Separation Merged Into a Single Automated<br />
Front-end System<br />
The universal applicability of MS as detection technique has been a great help in streamlining the lab organization.<br />
For sample preparation, though just as desirable, such a universal approach has not evolved so far, hampering<br />
the total aut<strong>omation</strong> and integration of front-end sample prep and LC-MS. To circumvent this problem, sample<br />
extraction is integrated with LC to permit direct injections of “raw” biological samples without prior filtration,<br />
centrifugation or protein precipitation. With this approach, LC, on-line SPE-LC (“XLC”) and method development<br />
can be performed without any change of hardware or MS connections. The potential of this new sample<br />
introduction approach for MS is demonstrated by examples of the various operating modes. In LC mode, the<br />
system can analyze standards or extracts obtained from validated LLE, protein precipitation and SPE procedures.<br />
XLC mode can be used to analyze untreated biological samples such as plasma directly. The benefits of this<br />
automated technique are fully explored, which means that, e.g., denaturing wash procedures were developed for<br />
a very efficient automated on-cartridge protein precipitation. Denaturing proteins on-cartridge minimizes matrix<br />
interferences such as ionization suppression, and also omits off-line protein precipitation and centrifugation.<br />
Also a generic XLC method is developed to eliminate method development (MD). For more demanding assays<br />
an automated protocol has been developed to simplify MD. The obtained data gives information on recovery,<br />
breakthrough and tubing adsorption of the drug to be analyzed. Moreover, the obtained data can be used to point<br />
out ionization suppression without applying post-column infusion experiments.<br />
TP032<br />
Morten Egeberg<br />
Dynal Biotech ASA<br />
Molecular Systems<br />
P.O. Box 114 Smestad<br />
Oslo, 0309 Norway<br />
morten.egeberg@dynalbiotech.com<br />
Co-Author(s)<br />
Ingrid Manger, Tommy Rivrud,<br />
Tine Borgen, Stine Bergholtz,<br />
Dag Lillehaug<br />
Magnetic Bead Based Automated Isolation of Polyhistidine-Tagged Proteins for Purification<br />
and Target Screening<br />
We have developed automated protocols for the isolation of recombinant polyhistidine-tagged proteins from<br />
bacterial lysates on ThermoLabsystem’s KingFisher 96 magnetic particle processor system, in addition to Tecan’s<br />
Genesis RSP and Beckman Coulter’s Biomek FX liquid handling robots. This method employs cobalt-based<br />
Immobilized Metal Affinity Chromatography (IMAC) using Dynal Biotech’s super-paramagnetic beads (Dynabeads ® )<br />
to which the BD Talon chemistry has been immobilized. Compared to technologies that employ nickel-based<br />
IMAC, Dynabeads ® Talon are able to bind polyhistidine-tagged proteins with an enhanced selectivity. In addition,<br />
bound polyhistidine-tagged proteins are able to be eluted using conditions less stringent than those needed when<br />
using nickel-based IMAC. The polyhistidine-tagged proteins can be eluted from the beads, or bound to the beads<br />
they can be used directly in downstream applications such as phage display screening, protein-protein interaction<br />
studies and drug-target screens. By setting up this protocol on a variety of robotic systems, covering medium to<br />
high throughput applications, we show that Dynabeads ® Talon have properties making them highly suitable for<br />
aut<strong>omation</strong>. Moreover, our results demonstrates the flexibility and amenability of the Dynabeads ® Talon.<br />
POSTER ABSTRACTS
TP033<br />
Xingwang Fang<br />
Ambion, Inc.<br />
Research and Development<br />
2130 Woodward Street<br />
Austin, Texas 78744<br />
xfang@ambion.com<br />
High Throughput RNA Isolation – Methods Comparison<br />
162<br />
Co-Author(s)<br />
Roy C. Willis<br />
Quoc Hoang<br />
Michael Siano<br />
The demand for high throughput RNA isolation has been dramatically increasing with wide application of RNAi,<br />
expression profiling and molecular diagnosis. We will present a comparison of various RNA isolation methods that<br />
have been adapted to high throughput platforms, focusing on consistently high yield and quality of isolated RNA,<br />
reduction of cross-contamination, and simplicity and robustness of the protocol. The most widely used method<br />
for high throughput RNA isolation uses glass fiber filter plate where RNA binds to filter in the presence of high salt<br />
and alcohol. New methods have been developed using microspheric beads. In general, microspheric bead-based<br />
approach results in more consistent RNA recovery than glass fiber filter based RNA method. This is because<br />
beads can be fully resuspended in solution to enable more thorough mixing, washing, and elution. In addition,<br />
bead-based method is easier to automate than filter plate-based method where vacuum may cause crosscontamination<br />
and occasional filter clog may fail all samples in the whole plate. We will show comparison results<br />
on RNA isolation from cultured cells, as well as for viral RNA isolation from liquid biological samples. We will also<br />
discuss a technology for generating cDNAs and performing PCR directly from cells. In this technology, nucleases<br />
and reverse transcriptase inhibitors are deactivated during a simple lysis step. The resulting cell lysate can be used<br />
directly to make cDNA and for one-step qRT-PCR, bypassing the RNA isolation step. Data from the application of<br />
this technology to siRNA-mediated gene silencing will be presented.<br />
TP034<br />
W. Steven Fillers<br />
TekCel, Inc.<br />
103 South Street<br />
Hopkinton, Massachusetts 01748<br />
steve.fillers@tekcel.com<br />
ENCOMPASS: A New Expandable Approach for Large-Scale, Multi-Format Sample<br />
Management<br />
Traditional approaches to automating large-scale sample storage, retrieval, and distribution have been limited<br />
to costly, customer engineered “cathedral” systems. Though these systems have met the capacity and process<br />
control demands of the select few, they present a number of significant implementation burdens and operational<br />
risks to many. Large-scale systems (>1M samples) are typically engineered to meet fixed capacity and throughput<br />
requirements, necessitating immediate capital investment to meet potential future storage needs. Custom<br />
development and construction of facilities to house the system is frequently necessary, further expanding the<br />
cost and time for system implementation that, by design, provides no cost-effective option for progressive<br />
expansion. Additionally, due to the complexity of these single-point-of-access systems, failures of individual<br />
robotic components can bring the entire sample management operation to a halt. To address these challenges<br />
TekCel has developed Encompass, an expandable, large-scale sample storage and retrieval system configurable to<br />
specific customer requirements. Encompass employs a versatile system architecture based on TekCel’s Universal<br />
Storage Module (USM). USMs serve as functional building blocks combined to provide the capacity and format<br />
scope (plates, tubes, vials, racks) required by the customer’s operational needs. Standard environmental control<br />
and robotic elements, established in TekCel’s modular sample management systems (TubeStore, PlateStore, etc.),<br />
are utilized to meet desired throughput and sample processing specifications while maximizing sample integrity.<br />
Encompass systems provide the highest degree of flexibility to address customer workflow requirements and allow<br />
staged implementation to support process change and manage capital outlay.
TP035<br />
Thomas Friedlander<br />
Foster-Miller, Inc.<br />
350 Second Avenue<br />
Waltham, Massachusetts 02451-1196<br />
tfriedlander@foster-miller.com<br />
Microfluidics Impact on Lab Aut<strong>omation</strong> and HTS<br />
We will look at the current technologies being developed in the world of microfluidics (Lab-On-a-Chip or LOC) and<br />
discuss the areas they will impact in the macro world of Lab Aut<strong>omation</strong> and HTS. Some HTS applications may be<br />
completely replaced by new developments while others may benefit and be helped along by increased screening<br />
capabilities presented by LOC solutions. We will identify how Lab Aut<strong>omation</strong> groups can help bring this new<br />
technology to their in-house customer base and help provide support and integration with current HTS solutions<br />
already in place. We will also discuss the broad nature of microfluidics R&D and the skill sets required to bring this<br />
new technology to the forefront. The discussion will include macromolecular crystallization technologies, HPLC,<br />
Genotyping, genetics, proteomics, cell based assays, proteins, labeling among other topics.<br />
TP036<br />
Sean Gallagher<br />
UVP, Inc.<br />
Product and Applications Development<br />
2066 W. 11th Street<br />
Upland, California 91786<br />
seang@uvp.com<br />
Co-Author(s)<br />
Alex Waluszko, Hui Zhang, John Wallace, and Kate Cole,<br />
UVP, Inc.<br />
163<br />
Molli Osburn, Scripps College<br />
Applications of a Highly Uniform UV Transillumination Imaging System for Quantitative DNA<br />
and Protein Analysis<br />
UV transillumination is a ubiquitous tool in Life Science research. With few exceptions, fluorescent stains used<br />
in post electrophoresis analysis of proteins and nucleic acids have significant excitation peaks with ultraviolet<br />
(300-365 nm) light, making midrange UV the excitation source of choice for high sensitivity analysis for many<br />
fluorophores. However, quantitative analysis is limited by the extreme lack of illumination uniformity across the<br />
surface of typical UV light boxes. We report the development of a highly uniform UV transillumination system.<br />
Through use of a high density lighting system with a tuned phosphor coating, uniformity of
TP037<br />
Alice Gao<br />
Corning Incorporated<br />
Corning Life Sciences-Applications<br />
2 Alfred Road<br />
Kennebunk, Maine 04043<br />
gaoa@corning.com<br />
Caco-2 Transport Assay Using New HTS Transwell ® 96-Well Permeable Supports<br />
164<br />
Co-Author(s)<br />
Debra Hoover<br />
Caco-2 cell monolayers grown on permeable transwell inserts are frequently used as an in vitro model for<br />
evaluating absorption properties, permeability and efflux transport properties of drug candidates in the drug<br />
discovery process. Such evaluations, as part of the ADME-TOX screening, are usually performed in the 24-well<br />
format. Recent advances in combinatorial chemistry and genomics have generated an unprecedented number<br />
of compounds needed for such testing, and have led to an increasing need for higher assay throughput. In this<br />
poster, we will describe the use of new HTS Transwell ® permeable supports in a 96-format using the Caco-2<br />
drug transport assay. A set of 7 drugs with known transport rates will be used to evaluate the permeability and<br />
differentiation level of Caco-2 cell monolayers grown on these 96-well transwell inserts. These results will be<br />
compared with those of 24-well transwell inserts. Performance of the 96-well transwell inserts with a robotic<br />
system will also be demonstrated using the Tecan Freedom Workstation which includes all of the fluid handling,<br />
robotics and incubator systems necessary to perform in vitro drug transport assays.<br />
TP038<br />
Carlos Garcia<br />
University of Texas at Austin<br />
Institute for Cellular and Molecular Biology<br />
1 University Station A4800<br />
Austin, Texas 78712-0159<br />
jackalccg@mail.utexas.edu<br />
Automated Aptamer Selection Against hnRNP A1 and its Proteolytic Derivative UP1<br />
Co-Author(s)<br />
Andrew D. Ellington<br />
J. Colin Cox<br />
Chi-Tai Chu<br />
One of the best studied and characterized core heterogeneous nuclear ribonucleoproteins (hnRNP) is hnRNP A1<br />
and its proteolytic derivative UP1. UP1 is a natural fragment of hnRNP A1 and lacks the glycine rich c-terminal<br />
region found in A1. Both proteins have been found to promote telomere elongation by recruiting telomerase in<br />
mammalian cells and both are active in pre-mRNA splicing. Because telomerase activity has been detected in the<br />
vast majority of human tumors, it is an attractive target for anticancer therapy.The method of automated in vitro<br />
aptamer selection can produce aptamers from random sequence populations of up to 10 15 unique sequences in<br />
a matter of days. In contrast, manual generation of aptamers is a tedious and very time consuming process. By<br />
using high throughput automated selection using a pool of random sequence RNA, high-affinity binding sequences<br />
were selected. The selected sequences were then used to assay telomerase activity in vitro. These binding<br />
sequences, or aptamers, can function as biosensors that can be used for diagnostic purposes. They can also be<br />
used to challenge antibody binding sites and can deactivate certain protein activities. Both hnRNP A1 and UP1<br />
have been found to bind to vertebrate single-stranded telomeric repeats in vitro; however, only UP1 can recover<br />
telomerase activity from a cell lysate. Therefore any difference in selected sequences can be used to determine<br />
how the glycine-rich region of hnRNP A1 absent in UP1 influences its binding characteristics and activities.
TP039<br />
Patrick Goertz<br />
University of Texas at Austin<br />
Institute for Cellular and Molecular Biology<br />
1 University Station A4800<br />
Austin, Texas 78712-0159<br />
goertz.p@mail.utexas.edu<br />
Automated Selection of Aminoglycoside Antibiotic Aptamers<br />
165<br />
Co-Author(s)<br />
J. Colin Cox<br />
Andrew D. Ellington<br />
The in vitro selection of aptamers that bind to low molecular weight targets is commonly a tedious, timeconsuming<br />
project. Aptamers are short (~80 nt) segments of nucleic acid that have been shown to mimic many<br />
properties of antibodies and which bind with high specificity and affinity to molecular targets. The process of<br />
selecting aptamers includes several rounds of combining the target with a randomized pool of nucleic acid,<br />
washing away the non-binding species, and amplifying the bound me<strong>mbers</strong>. Subsequent iterations of this process<br />
narrow down the nucleic acid pool to the strongest binding species. We have expanded current automated<br />
selection protocols to include aptamer selections against small molecules including the aminoglycoside antibiotic<br />
neomycin. This modified procedure decreases both the frequency of manual handling of the selection reagents<br />
and the time required to perform the experiment generating aptamers against the chosen target at a much greater<br />
rate. The method is suitable for integration with high throughput technologies, greatly expanding the possibility<br />
of discovering useful aptamers against other low weight targets. Such targets could include those with important<br />
diagnostic value such as neurotransmitters.<br />
TP040<br />
Norbert Gottschlich<br />
Greiner Bio-One, Inc.<br />
Maybachstrasse 2<br />
Frickenhausen72636 Germany<br />
norbert.gottschlich@gbo.com<br />
Mass Production of Plastic Chips for Microfluidic Applications<br />
Co-Author(s)<br />
A. Gerlach, G. Knebel,<br />
W. Hoffmann, A. E. Guber,<br />
Forschungszentrum Karlsruhe,<br />
Institut für Mikrostrukturtechnik<br />
Germany<br />
Commonly, microfluidic chips for medical diagnostics or life sciences are made from glass or silicon. Over the last<br />
years, however, polymers have gained great interest as substrates since they promise lower manufacturing costs.<br />
In addition, polymers are available with a wide range of excellent physical and chemical properties. For a low-cost<br />
production of microfluidic systems as single-use products, adequate manufacturing techniques are required. We<br />
have produced several microfluidic systems from various plastic materials, mainly for Capillary Electrophoresis<br />
(CE). The microchannel systems were either molded into the plastic substrate by vacuum hot embossing or<br />
produced by optimized injection molding. Routinely, mechanical micromachining was used to create the required<br />
metal molding tools. Extremely precise mold inserts could be generated by galvanic/lithographic processes.<br />
The CE chips have been used to separate both DNA fragments and mixtures of inorganic ions. Detection was<br />
carried out either by laser induced fluorescence (LIF) or by electrical methods. In the latter case, contact-free<br />
conductivity detection with electrodes located outside of the CE system was used. Microstructures have also been<br />
incorporated in a larger format that meets the standardized microplate footprint commonly used in high throughput<br />
screening (HTS). Microfluidic plates with 96 or 384 identical microstructures for applications in HTS, clinical<br />
diagnostics, and gene analysis have been manufactured as well.<br />
POSTER ABSTRACTS
TP041<br />
Joseph Granchelli<br />
NalgeNunc International<br />
Research and Design<br />
75 Panorama Creek Drive<br />
Rochester, New York 14260<br />
jgranch1@rochester.rr.com<br />
Anaylsis of Nucleic Acid and Protein Arrays on NUNC ArrayCote 16-Well Slides and<br />
96-Well Plates<br />
166<br />
Co-Author(s)<br />
Dan Schroen<br />
Tom Cummins<br />
Microarray technology, with its ability to produce a simultaneous profile of gene expression across tens-ofthousands<br />
of transcripts, is an extremely powerful technique in genetic analysis. However, given the high density<br />
and therefore large expense associated with high-density printed arrays and target material, sample replication<br />
is often limited for most researchers. Often, many thousands of the measured transcripts on a high density,<br />
slide- based array are not regulated under the experimental conditions of interest and add only expense rather<br />
than understanding. The first step in a series of experiments often involves determining the subset of regulated<br />
transcripts using high-density arrays. Smaller arrays can be used that cover the subset of transcripts found to<br />
be regulated under a given set of experimental conditions.These smaller, focused arrays offer a significant cost<br />
advantage, allowing a trade between the expense of high density and the statistical power from multiple replicates.<br />
By combining the replicate sampling possible in a chambered format with chemistry (APS, Epoxy, and Aldehyde)<br />
familiar to microarray users, Nunc has extended array technology in the form of 96-well plate and 16-well slide<br />
array platforms with excellent performance characteristics. With this format it is possible to spot either nucleic<br />
acids or proteins with excellent spot morphology (protein spots less than 250µm, PCR product spots less than<br />
120µm) and good binding capacity (saturated signals at less than 0.2µg/ml probe concentration). Background<br />
levels are low, yielding substantial signal/noise ratios.<br />
TP042<br />
David Griffin<br />
Genevac, Inc.<br />
711 Executive Boulevard, Suite H<br />
Valley Cottage, New York 10989<br />
dgriffin@genevacusa.com<br />
Combining Lyophilisation and Centrifugal Evaporation to Make a Fast Drying Process That<br />
Results in Rapidly Resuspendable Dry Solid Compound<br />
Centrifugal Evaporation is the solvent removal process of choice for high speed parallel drying of large nu<strong>mbers</strong><br />
of solutions in the 0.1 to 50 mls volume range, (particularly preparative HPLC fractions). However, lyophilisation is<br />
sometimes preferred because the final dried product may resuspend more easily and is less likely to adhere to the<br />
original vessel. Lyophilisation is however a very slow process taking as much as two days, unless a labor intensive<br />
“shell freezing” process is employed. Genevac have developed a hybrid process, which gives a lyophilised end<br />
product but is three-four times faster than conventional lyophilisation (i.e., possible overnight). A specially modified<br />
centrifugal evaporator is used, and racks can be handled straight from a fraction collector to the machine without<br />
the need for individual tube handling.
TP043<br />
Terri Grunst<br />
Promega Corporation<br />
Scientific Applications<br />
2800 Woods Hollow Road<br />
Madison, Wisconsin 53711<br />
tgrunst@promega.com<br />
MagneSil ® Total RNA High Throughput Isolation<br />
167<br />
Co-Author(s)<br />
Dan Kephart<br />
We have developed a novel total RNA isolation system that enables high throughput total RNA isolation without<br />
the need for centrifugation, vacuum filtration, or precipitation. The MagneSil ® Total RNA mini-Isolation System<br />
chemistry and format is designed for high throughput total RNA isolation in automated 96-well or 384-well formats.<br />
This novel system takes advantage of the unique properties of Promega’s MagneSil ® paramagnetic particle<br />
technology to isolate total RNA from complex biological mixtures. The automated total RNA isolation procedure<br />
takes as little as 30 minutes and includes a DNase step for removal of contaminating genomic DNA. 96-well total<br />
RNA can be isolated from
TP045<br />
Jennifer Halcome<br />
Eppendorf-5 Prime, Inc.<br />
Product Applications<br />
6135 Gunbarrel Avenue, Suite 230<br />
Boulder, Colorado 80301<br />
jhalcome@5prime.com<br />
168<br />
Co-Author(s)<br />
George Halley<br />
Gerry Huitt<br />
Plasmid DNA Purification Using the Eppendorf Perfectprep ® Plasmid 96 Vac Direct Bind Kit<br />
on the Eppendorf epMotion 5075 Workstation<br />
The Eppendorf epMotion 5075 workstation allows for full aut<strong>omation</strong> of a variety of applications. It is particularly<br />
suitable for the rapid purification of 96 plasmid preparations using the Eppendorf Perfectprep Plasmid 96 Vac<br />
Direct Bind kit. The protocol for this kit is provided, pre-programmed, with the epMotion 5075 workstation and<br />
results in excellent yields of high quality plasmid DNA. When sequenced, the DNA exhibits high passing rates<br />
and exceptional Phred Q>20 quality scores. The Eppendorf Perfectprep Plasmid 96 Vac Direct Bind kit and<br />
the epMotion 5075 provide a complete system for low and medium throughput isolation of plasmid DNA. The<br />
epMotion 5075 can be purchased with a variety of deck configurations that can include up to three temperaturecontrolled<br />
positions. In addition, the deck may incorporate either a vacuum manifold or a thermocycler. The<br />
versatility of the epMotion 5075 allows for the processing of a variety of applications including purification of BAC<br />
DNA using the Eppendorf Perfectprep BAC 96 kit and purification of PCR products using the Eppendorf PCR<br />
Cleanup 96 kit. Other applications include PCR and sequencing setup, thermocycling and restriction digests.<br />
TP046<br />
Paul Held<br />
Bio-Tek Instruments<br />
100 Tigan Street<br />
Winooski, Vermont 05404<br />
heldp@biotek.com<br />
Co-Author(s)<br />
Lenore Buehrer<br />
The ELx405 HT 384-Well Microplate Washer: Designed to Meet the Rigors of Biomolecular<br />
Screening<br />
In today’s HTS environment, 384-well microplates have become the preferred format, regardless of the screening<br />
assay type. Most often these assays require some sort of aspiration and/or dispensing of fluids to the wells of<br />
the microplate typically accomplished by a microplate washer. The ELx405 HT is a dedicated 384-well microplate<br />
washer that utilizes 192 pairs of aspiration and dispense-tubes to provide rapid and effective aspiration and<br />
dispensing of fluid without sacrificing features or performance. Using the same patented dual-action manifold<br />
design as the industry standard ELx405 Select washer, the ELx405 HT allows independent control, both vertically<br />
and horizontally, of the aspiration and the dispense manifolds. Several standard features have been included<br />
to make the ELx405 washer robotic-friendly and ideal for aut<strong>omation</strong>. Vacuum detection is provided to ensure<br />
that the vacuum pump is switched on and vacuum vessels are connected. Flow protection alerts the user to an<br />
interruption of flow to the dispensing manifold. Waste-level detection prevents overflow of waste into the vacuum<br />
pump. In addition to an overview of the ELx405 HT washer, data demonstrating the washer’s accuracy and<br />
precision of fluid-dispense, evacuation efficiency, as well as speed and timing will be included<br />
.
TP047<br />
John Helfrich<br />
NuGenesis Technologies<br />
Drug Discovery and Development Group<br />
1900 West Park Drive<br />
Westborough, Massachusetts 01581<br />
jhelfrich@nugenesis.com<br />
The Data Explosion: “Print-to-Database” Technology for the HTS Laboratory<br />
Managing and tracking data through the discovery, optimization and development, requires a compilation of many<br />
different types of inter-departmental analytical, biological, and document report outputs. Data collaboration from<br />
the multiple disciplines within the drug discovery environment across the entire enterprise, is critical to making<br />
crucial go/no go decisions regarding lead candidate development at all stages of the discovery process. This<br />
paper will focus on the use of an application-independent “print-to-database” technology that allows scientists,<br />
management and intellectual property personnel in a HTS environment to capture, catalog, view, and use the<br />
mountain of data for lead candidate program management.<br />
TP048<br />
Terry Hermann<br />
LabVantage Solutions, Inc.<br />
245 US Highway 22 West<br />
Bridgewater, New Jersey 08807<br />
thermann@labvantage.com<br />
Meeting the Needs of High Throughput Genetics-based Research<br />
169<br />
Co-Author(s)<br />
Terry Smallmon<br />
J. Kelly Ganjei<br />
Based on years of experience in accelerating the efforts of companies focused on high throughput genotyping,<br />
LabVantage has developed the Sapphire Genetics Accelerator. High throughput genotyping was a natural<br />
progression for Sapphire Informatics, which was initially developed for large-scale sequencing efforts, such as<br />
those at Monsanto. In order to accommodate the needs of groups performing large-scale genotyping and analysis,<br />
the accelerator was designed with several key features out of the box. Sapphire automatically organizes all<br />
sample and patient data by Project, Study, and Assay, and can flexibly store genetic variations, and export them<br />
in any format (e.g., linkage). In order to handle the different needs of bioinformaticists, Sapphire allows for the<br />
flexible assembly of contextual data surrounding the genetics variation. Eliminating the typical “hunt and peck”<br />
associated with volumes of data, Sapphire facilitates rapidly viewing genotypes in relation to population, disease,<br />
genetic region, or any other criteria that exists in the database. Easing the difficulty of analyzing tremendous<br />
volumes of genetics data, Sapphire integrates with Industry standard analytical packages such as Visual Genetics,<br />
Genehunter/2, SAGE, SAS, Pedcheck, Linkage, and Transmit. Additionally, through integration with these<br />
packages, expanding your analysis by linking up phenotypic components with genotypes becomes a trivial task.<br />
At the end of the day, and even before management requests updates, Sapphire provides real-time summaries<br />
of data from large datasets at the Project, Study, or Experiment level. In addition to mapping these details of the<br />
scientific process, Sapphire can track any level of detail about the resources being used.<br />
POSTER ABSTRACTS
TP049<br />
Darren Hillegonds<br />
Lawrence Livermore National Laboratory<br />
Center for Accelerator Mass Spectrometry<br />
7000 East Avenue, L-397<br />
Livermore, California 94551<br />
hillegonds1@llnl.gov<br />
Co-Author(s)<br />
John S. Vogel, Lawrence Livermore National Laboratory<br />
David Herold and Robert Fitzgerald, Veterans Affairs San<br />
Diego Healthcare System/University of California, San Diego<br />
High Throughput Measurement of 41 Ca by Accelerator Mass Spectrometry to Quantitate<br />
Small Changes in Individual Human Bone Turnover Rates<br />
Biochemical bone turnover markers suffer from large analytical and natural fluctuations (20 – 30%), making small<br />
differences in bone resorption impossible to resolve. This limits the clinical utility of such markers for individuals with<br />
the skeletal complications associated with many disease states (e.g., metastatic cancer, renal failure, osteoporosis).<br />
We are developing the capability to measure small changes (5 – 10%) in bone turnover rate in vivo by tagging the<br />
living skeleton with 41 Ca. This measurement will enable earlier diagnosis of pathological processes and interactive<br />
intervention with therapeutic agents, allowing modulation of a therapeutic agent to obtain the best individual result<br />
for a patient. Among the stable and radioactive calcium isotopes, only 41 Ca is useful for direct quantitation of bone<br />
turnover because it is extremely rare in nature and radiologically benign (105,000 y half-life, pure electron capture<br />
decay). In support of several ongoing research projects, we have simplified and streamlined the sample preparation<br />
methodology. This, combined with the highly automated analytical instrumentation, allows a single operator to<br />
prepare or run more than 100 samples per day – significantly more than other 41 Ca programs worldwide. We have<br />
also prepared new 41 Ca dose materials for oral and intravenous administration. This 41 Ca was provided from a<br />
1% 41 Ca solution used in preparation of our primary standard, laboriously purified through selective precipitation<br />
and ion exchange. Radiological purity was measured for both beta and gamma radiation – both were at typical<br />
background levels. The trace elemental content was also measured, assuring the overall purity of the dose material<br />
for human use.<br />
170
TP050<br />
Lynn Hilt<br />
The Ashvins Group, Inc.<br />
Bio IT Professionals<br />
8390 N W 53rd Street, Suite 200<br />
Miami, Florida 33166<br />
jberlin@ashvinsgroup.com<br />
Software Validation for Aut<strong>omation</strong> Projects<br />
171<br />
Co-Author(s)<br />
Terry Weeks<br />
Thoroughly characterized and dependable software is a critical component of laboratory aut<strong>omation</strong> projects. The<br />
use of software to automate and streamline laboratory operations include projects that 1) automate laboratory<br />
procedures with robotic systems; 2) consolidate data collection and analysis; 3) manage and share data collected<br />
from sophisticated drug discovery or clinical research processes and/or 4) interface instruments, systems and<br />
software. Often these projects involve custom applications or modifications of Off-the-Shelf (OTS) software<br />
products. It is clear that these systems must satisfy Good Laboratory Practices (GLP) requirements, but the<br />
scope and methods employed to validate computer related systems is highly subjective.Validation of computer<br />
related systems is often complex, and alternate approaches may be used. The appropriate validation effort will<br />
depend upon various factors, including the complexity of the system, intended use of the system, and whether the<br />
functions are critical or non-critical in the aut<strong>omation</strong> project. This poster identifies factors to be considered and<br />
steps to be taken in validation of Computer Related Systems used in GLP environments.Validation technology may<br />
be addressed through a variety of testing formats such as:<br />
• Installation and Operational Qualification (IQ/OQ)<br />
• Off-the-Shelf Software (OTS) Validation<br />
• Custom Software Validation and Verification (V&V)<br />
In conclusion, pertinent validation testing and documentation are required to demonstrate proper performance of<br />
Computer Related Systems used to support laboratory aut<strong>omation</strong> projects.<br />
TP051<br />
Randall Hoffman<br />
Invitrogen Corporation<br />
Screening Applications<br />
501 Charmany Drive<br />
Madison, Wisconsin 53719<br />
randy.hoffman@invitrogen.com<br />
Co-Author(s)<br />
Gerald Habenbacher, Tecan Austria<br />
Ion Channel Assay Development Using Voltage Sensor Probes on the GENios Pro<br />
Multifunctional Reader From Tecan<br />
Ion channels are important drug targets because of their critical role in nerve, cardiac, endocrine, and skeletal<br />
muscle tissues. The lack of sufficiently sensitive, high throughput screening systems has hampered research<br />
in this area. Voltage Sensor Probes (VSP) technology can be used with any ion channel target that changes<br />
membrane potential, and is therefore well suited for sodium, potassium, calcium, chloride, and ligand-gated ion<br />
channel research. The FRET-based detection method provides ratiometric results which significantly reduces errors<br />
arising from well-to-well variations in cell number, dye loading and signal intensities, plate inconsistencies, and<br />
temperature fluctuations. These combined features make VSP technology highly amenable for high throughput<br />
screening (HTS) applications. Assay development and therapeutic groups develop and validate ion channel assays<br />
prior to HTS and may have lower throughput instrumentation that may or may not be amenable to VSP technology.<br />
This abstract demonstrates the use of Tecan’s GENios Pro instrument as a suitable platform for development of<br />
VSP ion channel assays in both the pharmaceutical and academic environments.<br />
POSTER ABSTRACTS
TP052<br />
Dawn Marie Jacobson<br />
Veterans Affairs San Diego Healthcare System<br />
3350 La Jolla Village Drive<br />
San Diego, California 92161<br />
watermusicdoc@yahoo.com<br />
172<br />
Co-Author(s)<br />
David Herold<br />
Elevated Serum Total Protein as an Indicator of Chronic Viral Hepatitis and/or HIV Infection<br />
Background: The importance of diagnosing, treating, and preventing transmission of hepatitis B virus (HBV),<br />
hepatitis C virus (HCV), and human immunodeficiency virus (HIV) infection is well-known. An elevated serum total<br />
protein level may indicate an increase in immunoglobulins secondary to infection, autoimmune disease, hepatic<br />
compromise, the presence of a monoclonal gammopathy or malignancy. The relationship between an elevated<br />
serum total protein level and HBV, HCV, and/or HIV infection is unknown. Methods: Chart review and laboratory<br />
analysis of 96 consecutive patients with a protein level greater than 8.3 g/dL (reference range 6.4-8.3 g/dL) was<br />
performed. Laboratory analysis included serum total protein level, SPEP, HIV-1 ELISA, HBsAg, HBsAb, HBcAb, and<br />
HCV antibody testing. Results: 66 patients (69%) showed serologic evidence of one or more of these infectious<br />
diseases. 25% were new diagnoses (0 HIV, 6 HCV, 18 HBV). This represents a prevalence of HBV and HCV<br />
infection that is four times what would be expected in the San Diego veteran population. Conclusions: An elevated<br />
serum total protein level may be an indicator of inflammation secondary to HBV, HCV, or HIV infection. In the light<br />
of cost-effective medicine, and especially in areas where resources are limited, an elevated serum total protein<br />
level should be utilized as an additional screening modality to direct the diagnosis of unsuspected HBV, HCV,<br />
and HIV infection. The clinical use of a highly automated and inexpensive total protein screening test will insure a<br />
greater percentage of infected individuals will be identified, treated, and educated about the prevention of disease<br />
transmission.<br />
TP053<br />
Joong Hyun Kim<br />
University of California, Riverside<br />
Chemical and Envrionmental Engineering<br />
B148 Bourns Hall<br />
Riverside, California 92521<br />
jhkim@engr.ucr.edu<br />
Nano Crystal Hydride Stable DNA Probe<br />
Co-Author(s)<br />
Jared Stephens<br />
Dimitrios Morikis<br />
Mihrimah Ozkan<br />
Revolution in biochemistry and biomedical engineering has been boosted by the inventions of tools such as PCR,<br />
DNA chip, Biosensors and so on. DNA or RNA probe is the basic component of those tools because DNA or<br />
RNA has high stability and specificity to its targets. Among those probes, molecular beacons have distinguished<br />
ability to detect their specific targets in a sample containing even single base- mismatched targets. Furthermore,<br />
the novel probes do not require washing step to observe fluorescence when there is hybridization between the<br />
probes and the targets. Therefore, molecular beacons have been applied in various applications including realtime<br />
monitoring of polymerase chain reactions, developing DNA sensors, and in monitoring target RNAs in vivo<br />
for drug developing. However, for practical in vivo application of molecular beacons, limitation still remains due to<br />
the photobleaching characteristics of organic dyes and their available limited number of colors. Since the lifetime<br />
of organic dyes is not long enough, it is challenging for example to observe expression of target RNA in vivo.<br />
Furthermore, organic dyes have their own excitation wavelength, and they require multiple energy sources, which<br />
can cause energy accumulation in living cells. Since their spectrum’s overlap, it is difficult to observe two different<br />
colors at the same time and thus can limit the number of detectable target. Here we report for the first time a<br />
hybrid molecular beacon with nanocrystal an inorganic fluorophor and organic quencher that exhibits improved<br />
stability against photobleaching.
TP054<br />
Andreas Kuoni<br />
University of Neuchatel<br />
Institute of Microtechnology<br />
Rue Jaquet-Droz7<br />
Neuchatel, CH – 2007 Switzerland<br />
Andreas.Kuoni@unine.ch<br />
Multi-Channel Dispenser for Micro-Array Printing<br />
173<br />
Co-Author(s)<br />
Marc Boillat, University of Neuchatel<br />
Bart van der Schoot, Seyonic SA<br />
The ability to carry out assays in parallel in a micro array format is essential for the success of high throughput<br />
analysis systems. We report a new type of piezoelectric dispenser which dispense 65 pL droplets in parallel and<br />
in a 2D array format onto a micro-arrray at a 2D spacing of 500 µm. The dispenser is continuously loaded from a<br />
standard well plate with a well spacing of 4.5 mm during dispensing. An innovative technology using Polyimide<br />
sheets has been developed for the construction of a 2D micro-array printing device. The flexibility of the laminated<br />
Polyimide sheets allows assembling different sizes of dispensing arrays at a center-to-center spacing of 500 µm<br />
in a modular way by stacking flexible dispenser sheets (e.g., 32*48, 16*24, 8*12). The major advantages of the<br />
present device are no interface changes in the fluidic path, no tubing for liquid transportation, and no robotics<br />
system. The spotting has been characterized qualitatively and quantitatively. First we observe satellite free<br />
dispensing. The average droplet velocity is 1.79 m/s ± 0.2 m/s per line at a common driving signal of 180 Volts.<br />
The dispensed droplet has a nominal volume of 65 pL, calculated from the droplet diameter. The variation of<br />
dispensed volume between spotting sites is have been compared first by calculating the evaporation rate and<br />
then applying this to the droplet evaporation time. The average evaporation time for a single channel measuring 30<br />
successive droplets is t=1.40 sec ± 0.12 sec results in a CV value of 8.6 %.<br />
TP055<br />
Fred Lange<br />
University of Rostock<br />
Institute of Aut<strong>omation</strong><br />
R.-Wagner-Str. 31<br />
Rostock18119 Germany<br />
fred.lange@uni-rostock.de<br />
Data Mining and Visualization in Sports Medicine Aut<strong>omation</strong> by WebServer Oriented<br />
Software and WebBrowser Oriented User Interfaces<br />
Co-Author(s)<br />
Regina Stoll<br />
Reinhard Vilbrandt<br />
Laboratory data archives organized in databases play an important role in modern preventive medicine. Especially<br />
in Occupational Health and Sports Medicine huge amounts of data about human’s fitness are produced.<br />
Distributed data sources and acquisition systems do not allow to produce enough representive data about all<br />
possible populations in every lab. So, the idea was born to centralize databases and to identify physical fitness<br />
data by methods and algorithms on the fundaments of these centralized data. But, so the concept for data<br />
processing in data mining has to be centralized too. The strategy for this is based on Web-server running software<br />
components. The goal was to prevent local software components, except the operating system. This would be<br />
a big step in direction of ubiquitous computing – independed from the operating system. The problem’s solution<br />
is oriented to build data mining procedures in graphic object oriented software systems. Virtual instruments (VIs)<br />
in LabVIEW (National Instruments) are an excellent tool for interpretation and visualization procedures for data in<br />
Occupational and Sports Medicine. In the last five years many applications for data processing in Occupational<br />
and Sports Medicine have been developed in our lab based on local Virtual Instruments connected to real sensor<br />
systems. A way to solve the problem in general is an implementation of centralized server oriented operated<br />
LabVIEW applications connected to a Man-Machine-Interface performed by a Web-Browser. The paper shows<br />
some model-applications and the way to realize Web-Interfaces and Data mining with the Internet-Toolkit in<br />
LabVIEW. The system can be used to fill the database with data from collaborators via Internet and get them back<br />
the calculated results as an image or as an ASCII-data set.<br />
POSTER ABSTRACTS
TP056<br />
Joseph Machamer<br />
Molecular Devices<br />
1311 Orleans Drive<br />
Sunnyvale, California 94089-1136<br />
joe_machamer@moldev.com<br />
Luminescence Assays and FDA CFR 21 Part 11 Compliance With the New LMax II384<br />
Microplate Luminometer<br />
Luminescence microplate assays continue to grow in popularity as reagent kit vendors introduce new, highly<br />
sensitive assays. Many of these assays are performed in the laboratories of pharmaceutical and biotechnology<br />
companies where compliance with FDA CFR 21 Part 11 is a requirement. Here we report on the results of<br />
applications run on the recently introduced LMax II 384 microplate luminometer from Molecular Devices in a FDA<br />
CFR 21 Part 11 environment.<br />
TP057<br />
Dieudonne Mair<br />
University of California, Berkeley<br />
Department of Chemical Engineering<br />
727 Lattimer Hall<br />
Berkeley, California 94720<br />
dmair@uclink.berkeley.edu<br />
174<br />
Co-Author(s)<br />
Timothy Stachowiak, Jean Frechet, and Emily Hilder,<br />
University of California, Berkeley<br />
Frantisek Svec, Lawrence Berkeley National Laboratory<br />
Monolithic Modules in Micro Total Analytical Systems Rapidly Fabricated From Plastics<br />
We report on the development of microfluidic devices fabricated from plastics, placing a porous polymer monolith<br />
in the channels, and use of these devices in the field of proteomics. To achieve this, a robust nickel master for<br />
injection molding of the devices has been fabricated using the dry etching, electroplating, and molding (DEEMO)<br />
process. Optical and chemical properties of a series of potential thermoplastic polymers have been screened and<br />
the best candidate for the injection molding of microchips – cyclic olefin copolymer Topas 8007x10 – selected.<br />
Since the surface of this material is highly hydrophobic, surface modifications targeting an increase in hydrophilicity<br />
were explored using model systems. According to the contact angle measurements, photografting the surface<br />
with poly[(ethylene glycol) monomethacrylate] (PEGMA) chains afforded a hydrophilic surface similar to that of<br />
poly(ethylene glycols) and led to a significant reduction in protein adsorption. The preparation of rigid, porous,<br />
methacrylate-based monoliths that resist protein adsorption and functions as a static mixers/chemical reactors<br />
was also studied. Hydrophilic monoliths were prepared by adding 2-hydroxyethyl methacrylate (HEMA) to the<br />
polymerization mixture. The pore size, measured by mercury porosimetry, was shown to decrease as HEMA<br />
content was incrementally increased.
TP058<br />
Kirk Malone<br />
The University of Edinburgh<br />
The School of Chemistry<br />
Joseph Black Building, West Mains Road<br />
Edinburgh EH9 3JJ United Kingdom<br />
Kirk.Malone@ed.ac.uk<br />
The Design and Synthesis of High Affinity Ligands for Human Cyclophilin A<br />
175<br />
Co-Author(s)<br />
Nicholas J. Turner<br />
Malocolm Walkinshaw<br />
Cyclophilin A (CypA) is a member of the immunophilin family of proteins and a receptor for the immunosuppressant<br />
drug cyclosporin A (CsA). CypA also catalyses cis-trans isomerisation of peptidyl-prolyl (Xaa-Pro) amide bonds,<br />
biologically important in protein folding. CypA is a potential therapeutic target for areas including HIV replication<br />
and parasitic development. The use of a computational molecular docking programme to screen a virtual library<br />
of compounds identified dimedone (5,5-dimethyl-1,3-cyclohexanedione) as a potential ligand. A number of low<br />
molecular weight ligands have been synthesised based upon O-acylated dimedone and N-acylated aminodimedone.<br />
Routes to 4-alkylated dimedone were also investigated and derivatives synthesised. Ligands were<br />
screened for binding to CypA using mass spectrometry, and ligand:CypA complexes were observed under<br />
electrospray ionisation conditions. Recent work has focused on the synthesis of a combinatorial library of<br />
compounds to further investigate the ligand:protein interaction.<br />
TP059<br />
Justin Mecomber<br />
University of Cincinnati<br />
Department of Chemistry<br />
404 Crosley Tower<br />
Cincinnati, Ohio 45221-0172<br />
mecombjs@email.uc.edu<br />
Rapid and Cost-Effective Prototyping of Plastic Microfabricated Devices for Mass<br />
Spectrometry<br />
Co-Author(s)<br />
Douglas Hurd<br />
Patrick A. Limbach<br />
The increased interest in polymer-based microfabricated and microfluidics devices as alternatives to silica-based<br />
devices for bioanalysis that are generated by a molding process require the generation of metal molding masters.<br />
Traditionally, expensive or time-consuming approaches including LIGA, UV-Lithography or electrical discharge<br />
machining are used to generate metal molding masters. Here, we demonstrate that conventional CNC-mills, such<br />
as those readily available in university or private machine shops, can be used to generate metal features with<br />
tolerances up to a factor of 10 better than expected from the mill specifications. With such improved machining<br />
tolerances, a CNC-milling approach can now be used to generate high aspect ratio features suitable for lab-ona-chip<br />
devices. Hot embossing, in conjunction with solvent bonding, has been utilized to fabricate these polymer<br />
devices. An electrophoresis based detection system using fluorescence microscopy has been employed to<br />
determine the analytical capabilities of microchips generated in this manner. Comparisons with microchips made<br />
by molding fabrication methods such as wire imprinting and LIGA will be compared to microchips produced from<br />
the molds made by CNC-machining. These easily produced chips are of a suitable quality and design for use with<br />
analytical methods such as microchip-CE laser induced fluorescence or mass spectrometry.<br />
POSTER ABSTRACTS
TP060<br />
Gwendolyn M. Motz<br />
The University of Texas<br />
ICMB<br />
2500 Speedway, MBB 3.424<br />
Austin, Texas 78712<br />
gwenmotz@mail.utexas.edu<br />
Automated Optimization of Aptamer Selection Buffer Conditions<br />
176<br />
Co-Author(s)<br />
J. Colin Cox<br />
Andrew D. Ellington<br />
Optimizing the buffer conditions of the selection of nucleic acid species (aptamers) increases the likelihood<br />
of producing an anti-target aptamer. Aptamers, with high target affinity and specificity, are often compared to<br />
antibodies, as aptamers emerge in the industry as diagnostic and therapeutic tools. The increased demand for<br />
aptamers encourages high throughput aptamer generation. The selection buffer conditions may vary as widely as<br />
the selection targets, and therefore buffer optimization is helpful if not required for effective aptamer selections.<br />
Such optimization work is time consuming and repetitious, which bodes well for high throughput applications.<br />
To accommodate this, an automated buffer testing protocol has been developed to test target-to-unselected<br />
RNA pool binding in the presence of 96 different buffer conditions. Such a program requires very little pre-run<br />
preparations. The dynamic program may vary the monvalent salt(s) identity, monovalent salt(s) concentration,<br />
divalent salt(s) identity, divalent salt concentration, buffer identity, buffer concentration, and pH. The optimized<br />
buffer conditions likely increase the probability of a successful selection and therefore promote higher ratios of<br />
successful aptamer selections against a variety of targets.<br />
TP061<br />
Peyman Najmabadi<br />
University of Toronto<br />
Mechanical and Industrial Engineering<br />
5 King<br />
Toronto, Ontario M5S3L1 Canada<br />
najm@mie.utoronto.ca<br />
Co-Author(s)<br />
Andrew A. Goldenberg<br />
Andrew Emili<br />
Axiomatic Conceptual Design and Investigation of a New and Generic Laboratory Aut<strong>omation</strong><br />
Robotic Workcell With Application to Proteomics<br />
Proteomics (and Genomics) laboratories make use of experimental protocols that are highly repetitive and labour<br />
intensive. Furthermore, proteomics research is highly dependent on the productivity of a sizeable community<br />
of medium-sized (10 – 20 person) biochemical laboratories, which have limited resources. This paper presents<br />
the conceptual design, using the principles of axiomatic design theory, of a novel, generic laboratory robotic<br />
workcell optimized for increasing the productivity and efficiency (i.e., throughput) of these laboratories. Axiomatic<br />
theory defines the design process as the creation of solutions that satisfy specified requirements through<br />
systematic mappings between functional requirements (FR’s) and design parameters (DP’s) that are consistent<br />
with fundamental axiomatic principles which are Independence and Information axioms. Based on this theory, an<br />
optimal robotic workcell is conceptually designed to meet the major requirements of medium-sized biochemical<br />
laboratories: operational flexibility, reconfigurability, scalability, throughput and small footprint. The axiomatic design<br />
principles are used as an effective structural framework to model traditional configurations of laboratory aut<strong>omation</strong><br />
and to assess their shortcomings to fulfill the requirements of medium-sized laboratories. A robotic workcell is<br />
proposed by choosing appropriate design parameters for the functional requirements such that the overall design<br />
is decoupled. The workcell is generic and could be used for automating different biochemical protocols in mediumsized<br />
proteomics or related laboratories. Systematic design procedures of axiomatic theory has led to a design<br />
which outperforms traditional laboratory robotic workcells: (i) it has a higher value for throughput to footprint ratio;<br />
(ii) it is more protocol reconfigurable; and (iii) it is more scalable.
TP062<br />
Laura Pajak<br />
Beckman Coulter, Inc.<br />
Biomedical Research Division<br />
7451 Winton Drive<br />
Indianapolis, Indiana 46268<br />
laura.pajak@sagian.com<br />
Automated Proteomic Applications on Beckman Coulter’s Biomek ® NX Laboratory<br />
Aut<strong>omation</strong> Workstation<br />
177<br />
Co-Author(s)<br />
Chad Pittman<br />
Scott Boyer<br />
The information included in this poster describes the utilization of a new automated liquid handler, the Biomek<br />
NX Laboratory Aut<strong>omation</strong> Workstation, for proteomic applications. His-Tag proteins were purified from bacterial<br />
cultures in 96-well plates. Following purification, aliquots from the purified samples were quantitated on the<br />
Biomek NX using a Bradford assay. An integrated gripper on the span pod of the Biomek NX permits the microtiter<br />
plate containing the quantitation samples to be placed into an integrated reader, the AD340 ALP. This allows for<br />
complete walk-away aut<strong>omation</strong> of protein purification and quantitation. The following system components for the<br />
proteomic applications will be described:<br />
• The hardware requirements for the Biomek NX<br />
• Software and methods to drive the aut<strong>omation</strong> workstation.<br />
Results obtained when purifying proteins from both uninduced and induced bacterial cultures on the worksurface<br />
of the Biomek NX will be described.<br />
TP063<br />
Chad Pittman<br />
Beckman Coulter, Inc.<br />
Applications<br />
7451 Winton Drive<br />
Indianapolis, Indiana 46268<br />
chad.pittman@sagian.com<br />
Aut<strong>omation</strong> of Immunotech’s IL-8 ELISA Using Beckman Coulter’s Assay WorkStation<br />
Version 1.5<br />
Co-Author(s)<br />
Laura Pajak<br />
Scott Boyer<br />
Beckman Coulter, Inc. has developed and optimized an automated method using the Assay WorkStation Version 1.5<br />
to provide a complete, walk away system that automates ELISA assays. The system uses SAMI ® WorkStation<br />
scheduling software to optimize the use of incubations to make processing as efficient as possible. The method<br />
allows processing of up to three families (6 plates) in under five hours with minimal reagent waste. On-deck plate<br />
washing and reading are achieved by using an integrated Beckman Coulter MW96 washer and AD340 Automated<br />
Labware Positioner, respectively. Consumables are stored in an Automated Storage Module Cytomat Microplate<br />
Hotel HS with Plate Shuttle System and brought onto the worksurface by the Biomek ® FX Cytomat Automated<br />
Labware Positioner thereby allowing complete processing without user intervention.The information provided here<br />
will:<br />
• describe the automated system used to process the ELISA;<br />
• demonstrate the utility of the Assay Workstation;<br />
• describe the results when processing Immunotech’s IL-8ELISA kit on this system.<br />
POSTER ABSTRACTS
TP064<br />
Dominik Poetz<br />
National Institute of Standards and Technology<br />
Analytical Chemistry Division<br />
100 Bureau Drive<br />
Gaithersburg, Maryland 20899<br />
dominik.poetz@nist.gov<br />
Using the Analytical Information Markup Language (AnIML) to Represent LC-Diode-Array<br />
Data<br />
ASTM Subcommittee E13.15 has been formed, in part, to develop a standard markup language for spectroscopic<br />
and chromatographic result data. The Analytical Information Markup Language (AnIML) is being created using the<br />
Extensible Markup Language (XML) and is based on existing markup languages for analytical result data such as<br />
NIST’s SpectroML and Thermo’s GAML as well as data definitions and concepts from existing standards such as<br />
JCAMP-DX, the ASTM’s Andi standards, IUPAC definitions, etc. The initial work has begun to create the AnIML<br />
core, the schema representing the data and metadata common to all analytical spectroscopy and chromatography<br />
techniques. The E13.15 subcommittee has asked NIST to develop an example implementation of AnIML for data<br />
from liquid chromatography with diode array detection. This technique was chosen as a model because it is not<br />
overly complex, as contrasted with mass spectrometry or nuclear magnetic resonance spectroscopy, and because<br />
the datasets are inherently two-dimensional. The implementation will be created around the AnIML core and will<br />
consist of a “technique-specific” layer for LC-diode arrays that is vendor independent, along with enough of a<br />
“vendor-specific” layer to realize the application for at least one existing commercial instrument. The goal is to<br />
create a sufficient AnIML representation for data from a actual analytical technqiue to see what works and what<br />
needs revision, to demonstrate the power of the markup language approach, and to serve as a model for those<br />
writing technique specific layers for other analytical spectroscopy and chromatography domains.<br />
TP065<br />
Ketul C. Popat<br />
Boston University<br />
Department of Biomedical Engineering<br />
44 Cummington Street<br />
Boston, Massachusetts 02215<br />
kpopat@bu.edu<br />
178<br />
Co-Author(s)<br />
Tejal A. Desai and Gopal Mor, Boston University<br />
Craig Grimes, Pennsylvania State University<br />
Poly-Ethylene-Glycol Grafted Non-fouling Nanoporous Alumina Membranes<br />
Membranes currently used for separation of sub-micron particles in biomedical applications are of asymmetric<br />
or anisotropic variety and are made from polymers such as polysulfone or polyacrylonitrile. Several<br />
bioincompatibilities are associated with these polymeric membranes limiting their applications. Aluminum oxide<br />
substrates have been chosen for several reasons, including compatibility with our processing protocols, chemical<br />
and thermal stability, and ease in post-processing surface modification. Several studies have shown this material<br />
to be bioinert. However, the biocompatibility of the membrane material must be further investigated and improved.<br />
With proper surface modification, the protein adsorption can be lowered and the non-fouling characteristics can<br />
be improved. In this study we graft alumina membranes with a biocompatible polymer like poly (ethylene glycol) to<br />
investigate protein adsorption. Albumin and Fibrinogen were selected as model proteins since they are important<br />
components in immunogenic and thrombogenic reactions. X-ray photoelectron spectroscopy (XPS) and atomic<br />
force microscopy (AFM) were used to characterize PEG films on alumina membranes.
TP066<br />
Johannes Posch<br />
Tecan Austria GmbH<br />
Untersbergstrasse 1A<br />
GroedigA-5082 Austria<br />
johannes.posch@tecan.com<br />
Co-Author(s)<br />
G. Probst, K. Kratochwil, H. Bauer,<br />
R. Fuchs, G. Kreil, M. Köprunner,<br />
Austrian Academy of Science, Institute of Molecular Biology<br />
Aut<strong>omation</strong> of In Situ Hybridization on Tecan HS Series Hybridization Stations<br />
In situ hybridization (ISH) is a widely used technique in genome research to identify the function and interaction of<br />
genes and gene products in immobilized tissue sections. ISH provides a possibility to identify spatial and temporal<br />
activation of specific genes in specific diseases. With the HS 400 and 4800 Hybridization Stations, Tecan provides<br />
a series of instruments that are designed to fully automate microarray experiments but also to offer the flexibility<br />
to semi-automate in situ hybridization experiments. The HS Hybridization Stations eliminate manual errors and<br />
guarantee execution under constant conditions.<br />
TP067<br />
Shalini Prasad<br />
University of California, Riverside<br />
Electrical Engineering<br />
A 220 Bourns Hall<br />
Riverside, California 92521<br />
shals_prasad@hotmail.com<br />
Neurons as Sensors: Mixed Chemical Agent Sensing<br />
179<br />
Co-Author(s)<br />
Xuan Zhang<br />
Mo Yang<br />
Cengiz Ozkan<br />
Mihri Ozkan<br />
There is a need to develop small, highly sensitive, accurate, portable biosensors that can be used in real time<br />
situations which has the ability to distinguish between various chemical agents. Current methods rely on chemical<br />
properties or molecular recognition to identify a particular agent. These are limited in their capability to detect<br />
large number of possible agents both known and unknown, characterize the functionality of the known agents and<br />
predict human performance decrements. There is still a major gap in performing functional assays in the laboratory<br />
and implementing this concept in the field. This can be overcome by using cell based bio-sensors (CBB’s). We<br />
have designed and implemented novel CBB using single neurons as sensors that achieve single cell sensitivity and<br />
single agent selectivity using dielectrophoresis. We show here the capability of this sensor to accurately identify<br />
specific chemical agents from an environment containing a mixture of chemical agents.There is a special focus<br />
on sensing of gasoline and diesel. The chemical identification was performed by frequency domain analysis of the<br />
extracellular signal which generated a unique signature pattern vector corresponding to specific chemical agents.<br />
A time domain analysis was performed to determine the speed of response. The goal of the research is to develop<br />
a single cell sensor applicable in real time field conditions. The physiological changes in the cell due to the specific<br />
agent in terms of modifications to the ion channels are also determined simultaneously in a visual manner by the<br />
application of calcium and potassium dyes.<br />
POSTER ABSTRACTS
TP068<br />
Kirby Reed<br />
Gilson, Inc.<br />
Applications<br />
3000 West Beltline Highway<br />
Middleton, Wisconsin 53562<br />
kreed@gilson.com<br />
Evaluating and Determining Precision and Accuracy for Automated Liquid Handlers<br />
Dispensing Nanoliter Volumes of Viscous Solutions<br />
The thrust in today’s research is to smaller and smaller scale in order to complete a greater number of assays in<br />
a given amount of time leading to higher throughput and hopefully drugs to market. Micro scale research is not<br />
trivial, especially in regards to the dispensing of nanoliter volumes of viscous solutions and reagents with accuracy<br />
and precision. This application will demonstrate an evaluation procedure that will determine precision and accuracy<br />
for a series of viscous solutions dispensed in the nanoliters range via an automated liquid handler that employs<br />
non-contact solenoids for liquid aspiration and dispensing. The study is based on spectrophotometric analysis and<br />
will compare the results to the more commonly chosen weighing technique.<br />
TP069<br />
Laurent Rieux<br />
University of Groningen<br />
Pharmaceutical Analysis<br />
A. Deusinglaan 1<br />
Groningen9713 AV Netherlands<br />
l.rieux@farm.rug.nl<br />
NanoLC-MS analysis of TNFα in Microdialysates<br />
180<br />
Co-Author(s)<br />
Patty Mulder, Harm Niederlaender,<br />
Elisabeth Verpoorte, Rainer Bischoff<br />
TNFα is a small (17.4kDa) protein with pleiotropic activities related to cytotoxicity and inflammation. In order to<br />
study the role of TNFα in disease models in living animals, it is sampled locally by implanting a microdialysis probe.<br />
Due to the low concentrations expected (pg/mL) and the minute volumes of microdialysate, enhanced analytical<br />
sensitivity is required. We are therefore developing an analytical platform based on nanoLC-ESI-MS, which will<br />
be coupled with microdialysis for real-time, on-line analysis of TNFα in rats To this end, a nanoESI interface was<br />
built in-house. Electrical contact was made through a liquid junction by applying a high voltage on a stainlesssteel<br />
zero-dead-volume connection. The nanointerface was mounted on an xyz-micropositioner that allowed<br />
some rotational positioning. The performance of the nanointerface (spray stability, signal-to-noise ratio (SNR)) was<br />
studied with respect to positioning of the spray needle and eluent composition. Positioning the interface in front of<br />
and close to the MS inlet gave the highest signal intensity, whereas SNRs were optimal at longer distance. The use<br />
of distally coated needles gave the most consistent SNRs and it was easier to obtain a stable spray. At the same<br />
time, these needles required lower spray voltages and were robust. Better SNR and higher signal intensities were<br />
obtained with bare-silica needles, but they required more careful tuning. Therefore, distally coated needles were<br />
chosen for further work, which consisted of setting up a nanoLC system with a 75-µm-id analytical column. Work<br />
to analyze microdialysates spiked with TNFα to evaluate system sensitivity is underway.
TP070<br />
Kate Ross<br />
University of Leeds<br />
Chemistry<br />
Woodhouse Lane<br />
Leeds LS2 9JT United Kingdom<br />
kater@chem.leeds.ac.uk<br />
The Enhancement of Selectivity of a Pd-catalysed Three-component Cascade Reaction<br />
Using Automated Parallel Synthesis and Multivariate Data Analysis<br />
181<br />
Co-Author(s)<br />
Ronald Grigg<br />
Automated parallel synthesis with multivariate analysis is described for the optimization of a Pd-catalysed threecomponent<br />
cascade reaction of 7-buta-2,3-dienyl-1,3-dimethyl-3,7-dihydropurine-2,6-dione with iodobenzene<br />
and morpholine. Certain reactions of this type can yield a single isomer, although this is not the case with every<br />
reaction, the chemistry presented here being one where the selectivity of the reaction has not responded to routine<br />
one-factor-at-a-time optimization. Statistical experimental design is employed in the investigation of the effect of<br />
seven reaction factors on selectivity, yield and impurity levels. Reagent and phosphine stoichiometry, palladium<br />
source, time, temperature and concentration are included in the design, which is completed in 20 reactions on<br />
the Anachem SK233 Workstation with online HPLC monitoring. A solvent and base screen is then carried out<br />
using the predicted optimal conditions; these factors were previously controlled, as the substantial number of<br />
discrete variables involved is unsuited to the fractional factorial design. Instead, principal component analysis is<br />
used in selection of solvents, so gaining maximum variation in solvent properties and insight into which of these<br />
affect selectivity. Heterocyclic tertiary amines are chosen by pKa for screening as bases alongside alkali metal<br />
carbonates and acetates. This investigation will yield two-fold results, not only in enhancement of the reaction<br />
selectivity, but also information gained from a repeat design using optimized base and solvent conditions will reveal<br />
the importance of the sequence of experimentation if significantly different results are obtained.<br />
TP071<br />
Stephan Rudlof<br />
Fachhochschule Wiesbaden – University of Applied Sciences<br />
Computer Science<br />
Kurt-Schumacher-Ring 18<br />
Wiesbaden D-65197 Germany<br />
rudlof@informatik.fh-wiesbaden.de<br />
Robustness and Flexibility for Scheduling<br />
In a lab aut<strong>omation</strong> environment the real duration of an activity often cannot be predicted. This complicates<br />
the construction of a good scheduling algorithm. Say there is a sequence of device activities performed on<br />
different devices. If the successor of an activity has to be started immediately after its predecessor, the allocation<br />
time for its device needs to be long. This leads to the concept of time buffers between subsequent activities<br />
giving flexibility to the scheduler. As a consequence the resulting working plan will be robust against variability<br />
in execution times and will have short allocation times. This paper describes an approach needing very few<br />
parameters: minimal and maximal execution and delay times are sufficient. Just two delay parameters are sufficient<br />
to model all kind of delays from a fixed time delay (if time is zero, there is no delay) to a very flexible spring-like<br />
one. Together with the two activity execution time limits it is possible to keep the reservation times for devices low.<br />
Finally an outlook to a generalization to higher order activities follows: if there is a recursive definition of activities,<br />
higher order activities are composed of sub-activities, which may be composed, too; at the leafs there are atomic<br />
(device) activities. The time buffer approach above can be used at different levels in such an activity tree. This<br />
approach may be useful to develop powerful scheduling algorithms.<br />
POSTER ABSTRACTS
TP072<br />
Burkhard Schaefer<br />
National Institute of Standards and Technology<br />
Analytical Chemistry Division<br />
100 Bureau Drive; Building 227; Room A-159<br />
Gaithersburg, Maryland 20899-8394<br />
burkhard.schaefer@nist.gov<br />
Creating the Analytical Information Markup Language (AnIML)<br />
182<br />
Co-Author(s)<br />
Gary Kramer<br />
Multiple, vendor-specific, proprietary data formats have made it difficult to interchange analytical result data<br />
between instruments and applications. When data are interchanged, metadata are rarely included, often<br />
rendering the datasets useless after the particulars of the experiment have been forgotten. The few existing<br />
data exchange standards are not compliant with modern network-based data interchange technologies. A few<br />
years ago NIST began to develop a markup language for uv/visible spectrometry data based on the Extensible<br />
Markup Language (XML). The result, SpectroML, showed the power of this approach and is now integral to<br />
NIST’s data handling in its optical filter Standard Reference Materials program.ASTM subcommittee E13.15 on<br />
Analytical Data Management was formed in part to develop a general markup language, such as SpectroML, for<br />
all spectroscopic and chromatographic result data. Work has begun on the initial task to develop a core schema<br />
representing the information that is common to all analytical techniques. If a general core can be developed and<br />
adopted, standard applications can be written to utilize this core and accomplish simple functions such as data<br />
visualization, importing data into applications such as spreadsheets, etc. With a simple applet a web browser such<br />
as Microsoft’s Internet Explorer could display any spectral or chromatographic trace. More sophisticated concepts<br />
such as integrating peak areas, leveling baselines, etc. will require more complicated software, but having a single<br />
technique-independent routine to view data, paste the visual image into reports, or import data into other programs<br />
for further processing will be of enormous utility. This presentation will describe the current status of the AnIML<br />
development efforts and point out possible uses in various application domains.<br />
TP073<br />
Sadhana Sharma<br />
The Ohio State University<br />
Davis Heart and Lung Research Institute (326 HLRI)<br />
473 W. 12th Avenue<br />
Columbus, Ohio 43212<br />
sharma.119@osu.edu<br />
Rapid and Sensitive Determination of Cardiovascular Drugs in Mouse Plasma Using<br />
Solid-phase Extraction and RP-HPLC<br />
Co-Author(s)<br />
John Shapiro<br />
Stephen C. Lee<br />
Mauro Ferrari<br />
Fast and sensitive determination of the drugs is essential for pharmaceutical aut<strong>omation</strong> and disease management.<br />
Capillary electrophoresis, liquid chromatography–mass spectrometry (LC–MS), immunoassays and high-performance<br />
liquid chromatography (HPLC) are some of the methods used for drug analysis. Of these, the HPLC methods<br />
have been used most frequently because of their simplicity, sensitivity, and selectivity. Drug analysis from blood/<br />
plasma samples requires sample pretreatment before actual HPLC analysis. This can be achieved using solidphase<br />
extraction (SPE), protein precipitation and SPE, liquid–liquid extraction (LLE), solvent extraction followed by<br />
column-switching, or solvent extraction and SPE. Higher number of pretreatment steps decreases efficiency and<br />
increases analysis time. In addition, most of these HPLC methods require relatively large (a maximum of 0.5–1.0 ml)<br />
biological sample and multiple extraction steps. In cases where available sample volumes are in microliters, the<br />
HPLC methods with single LLE are generally not considered sensitive enough due to the presence of endogenous<br />
interferences. Solid phase extraction can be useful for such cases. In the present research effort, a rapid, simple and<br />
sensitive method for the determination of simvastatin in mouse plasma is developed. This method utilizes Waters<br />
OASIS solid-phase extractor and Symmetry C8 reversed phase high-performance liquid chromatography column<br />
for micro-sample analysis and is useful for analysis of the drug released from microfabricated silicon nanoporous<br />
implant in mouse/human.
TP074<br />
Letha Sooter<br />
The University of Texas at Austin<br />
Chemistry and Biochemistry<br />
1 University Station A5300<br />
Austin, Texas 78712<br />
letha@mail.utexas.edu<br />
Automated Double-stranded DNA Aptamer Selections<br />
183<br />
Co-Author(s)<br />
Andrew Ellington<br />
In vitro, double-stranded DNA aptamer selections were automated using a Tecan Genesis workstation. Aptamers<br />
are nucleic acid species that bind a target molecule with a high affinity and specificity. The selection process<br />
begins with a randomized pool of approximately 10 14 different double-stranded DNA molecules. Non-binding<br />
species are partitioned away, while species that bind the target molecule are amplified through the polymerase<br />
chain reaction. The iterative rounds result in the preferential enrichment of those binding species with the highest<br />
affinities. Post-selection, the pools are cloned and sequenced. A double-stranded DNA aptamer selection<br />
against the nuclear factor kappa B (NF�B) p50 homodimer was automated as a proof-of-principle. NF�B is a well<br />
characterized transcription factor that is known to bind a specific family of double-stranded DNA sequences. The<br />
results of the automated selection strongly correlate with previous manual selections against the same target.<br />
Following six rounds of selection, 93% of the sequences contained a general NF�B family binding sequence.<br />
The automated process also produced a significant increase in throughput. A manual round of selection requires<br />
several days of work, while an automated round requires only four hours. Presently, selections are being performed<br />
against multiple transcription factors in parallel. The selection process is used to identify the double-stranded DNA<br />
binding sequence of these proteins. With this information genomes can be searched for these binding sequences,<br />
thereby identifying information about the transcription factor function in vivo.<br />
TP075<br />
Henrik Schiøtt Sørensen<br />
Risø National Laboratorium<br />
Optics and Fluid Dynamics<br />
Frederiksborgvej 399<br />
Roskilde4000 Denmark<br />
henrik.schioett.soerensen@risoe.dk<br />
Fabrication of a Polymer Based Bio-sensing Optical Component<br />
Co-Author(s)<br />
Niels B. Larsen and Peter E. Andersen, Risø National Laboratorium<br />
Darryl J. Bornhop, Vanderbilt University<br />
A disposable optical biosensor is the objective of this research. The work presented here is based on a simple<br />
phenomenon, which may provide high sensitivity towards dissolved analytes in a polymer based micro fluidic<br />
system. Micro interferometric backscatter detection (MIBD) was first described by Bornhop et al. (Darryl J.Bornhop,<br />
Applied Optics 34[18], 3234-3239. 20-6-1995.) and has since proved to be able to detect changes in the refractive<br />
index in the 10 -7 regime. Absolute measurements of the refractive index have previously been demonstrated<br />
experimentally with a precision of 2.5 x 10 -4 in refractive index (H. S. Sorensen, H. Pranov, N. B. Larsen, D. J.<br />
Bornhop, and P. E. Andersen, Analytical Chemistry 75, 1946-1953 (2003)). The flow channel itself constitutes the<br />
optical element involved, hence great efforts are exerted to manufacture the flow channels with high accuracy.<br />
The geometry of the channel is similar to a half circle shape. Photolithography is used for the etching mask. The<br />
etching is performed with HF into fused silica to attain isotropic etching. The resulting groove is to be electroplated<br />
for injection molding of polymers. The fabricated microstructures are characterized by techniques such as confocal<br />
microscopy. Computer simulations of the system are presented concerning the demand on fabrication technique<br />
performance. Fabricated structures are shown as well. Our work showed that underetching of the patterned<br />
openings in the etch mask leads to formation of trapezoidal rather than semicircular grooves.<br />
POSTER ABSTRACTS
TP076<br />
Duraisamy Sridharan<br />
Anna University<br />
Ramanujan Computing Centre<br />
Chennai, Tamil Nadu 600025 India<br />
sridhar@annauniv.edu<br />
A Generic Model for Timely Refreshing of Very Large Semi-Dynamic Web Sites<br />
184<br />
Co-Author(s)<br />
P. Sakthivel<br />
S. K. Srivatsa<br />
The popularity of the World-Wide Web has made it a prime vehicle for disseminating information. More and more<br />
corporations and individuals advertise themselves through web sites in recent years. Although there has been<br />
much work on WWW information management, research in keeping information on web sites up-to-date is still<br />
in its infancy. Usually, all Web pages can be classified into three categories as follows: 1. Static: This kind of Web<br />
pages present information that either does not change over time, or it rarely changes. 2. Dynamic: The content of<br />
these Web pages varies according to various input parameters. 3. Semi-dynamic: These Web pages have their<br />
contents derived from some source databases and they change in response to updates to the source databases.<br />
In relative to dynamic pages, we refer to them as semi-dynamic pages because they remain static and do not<br />
change automatically unless a user initiates a refresh request explicitly. As the WWW continues its rapid growth,<br />
the number of semi-dynamic Web pages with information extracted from source databases increases rapidly too.<br />
A crucial problem arises when base data change at a high frequency and there is a need to keep a large set of<br />
semi-dynamic pages up-to-date in response to the changes since nobody is interested in stale data on the Web. In<br />
this paper, we study this scenario as a website refresh problem. We propose a generic model for timely refreshing<br />
semi-dynamic web sites, and discuss several important research issues involved in the field. A good solution to<br />
this problem is especially important for Genomic information management, electronic commerce applications, and<br />
other database-driven applications.<br />
TP077<br />
Claudia Stewart<br />
NCI-FCRDC<br />
MTC<br />
P.O. Box B<br />
Frederick, Maryland 21702<br />
stewart@ncifcrf.gov<br />
Automated Process of Q-PCR/Gene Expression<br />
Co-Author(s)<br />
James M. Cherry, Kelly T. Martin,<br />
Naryan K. Bhat, David Munroe<br />
The use of high throughput aut<strong>omation</strong> for the study of gene expression is currently under development. New<br />
large scale assays for genotyping, pathogen detection, viral load expression, and drug effects on cells are either<br />
underdevelopment or currently in use. The growing demands in the field of genomics require the development of a<br />
high throughput production for both quality RNA and its associated cDNA.This will allow for the use of a sensitive<br />
PCR based fluorescent detection assay, like Taq Man to be used high throughput. While aut<strong>omation</strong> solutions<br />
are readily available for various methods such as, RNA extraction, preparation of first strand synthesis cDNA,<br />
and running multiple Q-PCR, and integrated approach in linking all of the methods on a robotic platform is still<br />
forthcoming. Our progress in developing such a format is presented here.
TP078<br />
Regina Stoll<br />
University of Rostock<br />
Institute of Occupational Medicine<br />
St. Georg Str. 108<br />
Rostock18055 Germany<br />
regina.stoll@med.uni-rostock.de<br />
Fuzzy Based Medical Expert System for Automated Data Interpretation in Occupational<br />
Medicine<br />
185<br />
Co-Author(s)<br />
Mohit Kumar<br />
Norbert Stoll<br />
In medical data processing often there is a problem to formulate algorithms for data interpretation. In occupational<br />
medicine i.e., a number of different parameters are acquired for giving an idea to an expert about the status of<br />
physical fitness. The paper describes an neuro-fuzzy approach as a data mining tool for determination of physical<br />
fitness of a patient on a virtual scale between 0 and 1. The advantage of this procedure is, that there is no need for<br />
an analytical description of the complex functionality of physical fitness. So, the network is trained by original data<br />
and are able to fit in new data points on demand. The data used in this system are respiratzory parameters and<br />
heart rate parameters in combination with body type and body fat measurement data. By this way, the knowledge<br />
of an medical expert is combined with the individual parameter levels. The whole system is an artificial medical<br />
expert system for data interpretation.<br />
TP079<br />
Yu Suen<br />
Beckman Coulter, Inc.<br />
Biological Systems Operation<br />
4300 N. Harbor Boulevard<br />
Fullerton, California 92834<br />
ysuen@beckman.com<br />
Co-Author(s)<br />
Keith Roby, Javorka Gunic<br />
Michael H. Simonian, Graham Threadgill<br />
An Automated, High Throughput Cell-Based Assay System Using the Biomek 3000<br />
Laboratory Aut<strong>omation</strong> Workstation and CellProbe HT Caspase 3/7 Whole Cell Assay<br />
Using Beckman Coulter’s CellProbe HT Caspase 3/7 Whole Cell Assay in primary or secondary screening provides<br />
quality information on cellular responses to apoptotic regulators. This 35 µL total volume homogenous assay<br />
measures caspase 3/7 activities in a 384-well format. The high throughput and low sample volume cell-based assay<br />
is achieved using the Biomek 3000 Laboratory Aut<strong>omation</strong> Workstation for cell preparation, apoptosis induction and<br />
substrate addition. The Affinity Multi-Mode Reader (Cambridge Research Institute, Boston) is an ultra-sensitive<br />
screening platform that uses a focused laser and CCD camera for fluorescent intensity detection. The caspase<br />
3/7 activity is inducer dose-dependent and responder cell number-dependent. Using this system, a strong signal<br />
and a z´ factor of 0.5 were achieved with a minimal cell number. The dose response of DEVD-CHO inhibition of<br />
caspase 3/7 activities was used to demonstrate the superior sensitivity and specificity of this system. The Biomek<br />
3000 Laboratory Aut<strong>omation</strong> Workstation facilitated CellProbe HT Caspase 3/7 Whole Cell Assay implementation,<br />
reduced the chance of contamination and minimized the intensive requirement of sterile skills. The automated<br />
CellProbe assay can be used for high throughput screening of drug candidates for apoptosis regulators.<br />
POSTER ABSTRACTS
TP080<br />
Sarah Tao<br />
Boston University<br />
Biomedical Engineering<br />
44 Cummington Street<br />
Boston, Massachusetts 02215<br />
stao@bu.edu<br />
Polymeric Microdevices for Applications in Targeted Drug Delivery<br />
186<br />
Co-Author(s)<br />
Ka Wah Lee and Tejal Desai, Boston University<br />
Mike Lubeley, University of Illinois, Chicago<br />
The rapid development of macromolecular biopharmaceuticals has placed increasing interest on advanced carrier<br />
systems for efficient oral drug delivery. Advances in microfabrication technology have enabled the creation of<br />
entirely new classes of drug delivery devices which can possess a combination of structural, mechanical, chemical,<br />
and electronic features to surmount the challenges associated with conventional delivery systems. In this research,<br />
the strengths of microfabrication and micromachining are capitalized to create a completely novel, multifunctional<br />
microdevice from poly(methyl methacrylate) for potential applications in highly localized, tissue-specific oral drug<br />
delivery. PMMA drug delivery microdevices can be precisely manufactured using traditional microfabrication<br />
techniques. The PMMA is chemically modified to introduce surface amine groups to which biologically active<br />
molecules, such as avidin, can be covalently linked utilizing traditional carbodiimide coupling reagents. By further<br />
attachment of tomato lectin molecules, the PMMA is rendered cytoadhesive towards Caco-2 cells. PMMA<br />
devices are specifically designed flat and thin to maximize contact area with the intestinal lining. This flat design<br />
also minimizes the side areas exposed to the constant flow of liquids through the intestine. Corresponding in<br />
vitro studies have shown that these modified microdevices have increased Caco-2 cell recognition over control<br />
particles, as well as increased anchorage in comparison to PMMA microspheres. In addition, the presence of<br />
mucus proteins do not create as great an impact on the cytoassociation of these modified PMMA microdevices<br />
as on the control devices. In conclusion, these findings demonstrate the potential advantages of utilizing<br />
micromachined, asymmetrically cytoadhesive PMMA devices for applications in oral drug delivery.<br />
TP081<br />
Robert Umek<br />
Meso Scale Discovery<br />
9238 Gaither Road<br />
Gaithersburg, Maryland 20877<br />
rumek@meso-scale.com<br />
Co-Author(s)<br />
Lisa Gazzillo, Anu Mathew,<br />
Malcolm Smith, Timothy Schaefer,<br />
Jacob N. Wohlstadter<br />
A High Throughput Replacement for Western Blots: Protein Expression Analysis Using the<br />
MSD Multi-Array Platform<br />
We describe high throughput protein expression assays that are quantitative, rapid and sensitive. These assays<br />
provide alternatives to existing methods such as Western blot analysis and immunofluorescence techniques, which<br />
are labor-intensive, low in throughput, and time-consuming. In one procedure, cell lysates are adsorbed directly in<br />
the wells of MSD Multi-Array microplates; another format uses specific capture antibodies to increase sensitivity.<br />
Both procedures detect specific proteins by using an antibody labeled with an electrochemiluminescence reporter.<br />
We present two examples that demonstrate the utility of these assays: the identification of HEK293 clones that<br />
over-express the human vascular endothelial growth factor receptor 2 (VEGFR2), and the detection of epidermal<br />
growth factor receptor (EGFR) in an established carcinoma cell line. The assay can also be used to quantify the<br />
abundance of particular post-translational states of proteins of interest.
TP082<br />
Surekha Vajjhala<br />
Nanostream, Inc.<br />
580 Sierra Madre Villa Avenue<br />
Pasadena, California 91107<br />
surekha.vajjhala@nanostream.com<br />
Micro Parallel Liquid Chromatography for High Throughput ADMET Profiling<br />
187<br />
Co-Author(s)<br />
Li Zhang, Paren Patel, Jeffrey Koehler,<br />
Steve Hobbs, Chris Phillips<br />
The Nanostream Veloce system – which includes an instrument, software, and replaceable microfluidic cartridges –<br />
incorporates pressure-driven flow to achieve chromatograms comparable to conventional HPLC instrumentation<br />
while offering a dramatic increase in sample analysis capacity. The system enables parallel chromatographic<br />
separations and simultaneous, real-time UV detection for rapid determination of ADMET properties. Each<br />
Nanostream Brio cartridge, made of polymeric materials, incorporates twenty-four columns packed with standard<br />
(C-18) stationary phase material to achieve reverse phase separations. Mixing and distribution of the mobile phase<br />
to each of the 24 columns is precisely controlled in each cartridge. The system provides an ideal platform to<br />
accelerate assessment of physicochemical properties (i.e., log P, CHI, etc.) for a large number of compounds.This<br />
poster demonstrates how the Veloce system offers specific benefits for rapid assessment of ADMET parameters.<br />
The 24-fold increase in sample analysis capacity allows standard curve generation and simultaneous analysis<br />
of multiple replicates of samples in a single run. By accelerating access to high quality data, the Veloce system<br />
enables scientists to make decisions about promising leads earlier in the drug discovery process.<br />
TP083<br />
Ian Whitehall<br />
TTP LabTech Ltd<br />
Melbourn Science Park, Cambridge Road<br />
RoystonSG8 6EE United Kingdom<br />
inw@ttplabtech.com<br />
Cell Based Assays in 384- and 1536-Well Formats using MosQuito and the Acumen<br />
Explorer<br />
Co-Author(s)<br />
Paul Wylie<br />
Rob Lewis<br />
Wayne Bowen<br />
There is a growing interest in using whole cell assays in screening. By using a 1536-well format, the quantities<br />
of expensive compounds can be substantially reduced having a major impact on the cost of a screen. There are<br />
currently a number of technologies that dispense cells into 384-well formats, but fewer that are able to dispense<br />
viable cells into a 1536-well plate.In this study, we have used the MosQuito liquid handling system, which uses<br />
disposable pipettes, to dispense cells into 384- and 1536-well plates. We have then used the Acumen Explorer<br />
laser scanning fluorescence microplate cytometer to study proliferation of the cells over several days. We have also<br />
shown that we can determine cell cytotoxicity, as an example of a whole cell assay. The results demonstrate that<br />
MosQuito can successfully dispense viable cells into 384 and 1536 microtitre suitable for detection and assay with<br />
the Acumen Explorer.<br />
POSTER ABSTRACTS
TP084<br />
Hayley Wu<br />
Caliper Technologies Corp.<br />
Assay Development<br />
605 Fairchild Drive<br />
Mountain View, California 94043<br />
hayley.wu@calipertech.com<br />
Co-Author(s)<br />
Javier Farinas, Charles Park, Cheryl Cathey, Christopher Tsu,<br />
and Michael Pantoliano<br />
Lawrence Dick, Millennium Pharmaceuticals<br />
A High Throughput Microfluidic Protease Substrate Identification Assay<br />
Enzymatic assay development is often hampered by the lack of good substrates. We have developed a high<br />
throughput substrate identification assay based on a microfluidic chip. A series of fluorogenic substrates is sipped<br />
onto a chip and split into two parallel channels, one of which contains enzyme. Enzymatic activity on the substrate<br />
leads to a change in fluorescence. The difference in fluorescence between the enzyme channel and the reference<br />
channel is used to calculate the efficiency of the substrate for the target protease. The use of microfluidic channels<br />
gives excellent control of the timing and volume of reagent addition, making it possible to compare the enzyme<br />
channel with respect to a control. This improves data quality and allows for the optimization of substrates. The<br />
assay was validated using trypsin and a library of AMC labeled tripeptides. The results were consistent with the<br />
known amino acid sequence specificity of trypsin.<br />
TP085<br />
Hongkai Wu<br />
Stanford University<br />
518 Everett Avenue, #A<br />
Palo Alto, California 94301<br />
hongkai@stanford.edu<br />
On Chip Chemical Assay by Forming Droplets in Fluidic Systems<br />
188<br />
Co-Author(s)<br />
Richard N. Zare<br />
A goal of micro-total analysis systems (mTAS) is the manipulation and analysis of minute amount of biological<br />
samples in the order of pL volume, i.e., the size of most individual cells. Here, we demonstrate an integrated<br />
microfluidic system that was used to fast mixing of chemicals, on-column incubation of reactions without diffusion<br />
(e.g., labeling fluorescence tags and lysis of cells) and detection and analysis of final products. The system was<br />
fabricated in poly(dimethylsiloxane) (PDMS) and the fluid flow was driven by pressure. Samples in aqueous<br />
solutions were brought together and segregated into discontinuous droplets by another flow of immiscible fluid.<br />
These two parts in water droplets were mixed rapidly (ms) through a zigzag channel with pressure-driven flow.<br />
After fully mixed, the flow was stopped for reaction incubation before laser-induced fluorescent signals were<br />
measured. The two immiscible phases of droplets in the microchannels effectively prevented diffusion of chemicals<br />
during mixing and on-column incubation, which could require long time. Two different assays were performed in<br />
our chip. One is the enzyme assay of ß-galactosidase (ß-Gal) using resorufin ß-galactopyranoside (RBG) as its<br />
substrateand. The other assay is the determination of the concentrations of biological species. Each droplet had a<br />
volume of ~100 pL, and the number of molecule that are assayed was in the order of fmole in a droplet. The small<br />
volume of the droplets makes this system an excellent candidate for analysis the contents in individual single cells<br />
(volume in the order of pL), which we will do next.
TP086<br />
Wendy Yang<br />
FreshGene, Inc.<br />
2076 Beechwood Way<br />
Antioch, California 94509<br />
wendy@freshgene.com<br />
Co-Author(s)<br />
Qinghong Yang, Sandra Hatcher, and Henrietta Seet,<br />
FreshGene, Inc.<br />
Jeffrey Gregg, University of California, Davis – Medical Center<br />
Application of Holliday Junction Based Allele-Specific (HAS) Genotyping Platform for<br />
Detecting Two Common Thrombophilia Genetic Mutations<br />
The Factor V Leiden mutation (G1691A) and the Factor II (G20210A) mutation are the two most common genetic<br />
risk factors for venous thrombosis. Factor V Leiden mutation is present in 5% of the Caucasian population<br />
and the Factor II (G20210A) mutation is present in 2 % of the general population. We report the development<br />
of a novel diagnostic assays for detecting the Factor V Leiden mutation and the Factor II (G20210A) mutation<br />
based on a new mutation detection platform developed by our group: Holliday Junction based Allele Specific<br />
(HAS) genotyping (Yang et al, 2003). A mismatch at a point mutation site between the target PCR amplicons<br />
and reference PCR amplicons will lead to stable Holliday Junction structure formation which can be detected<br />
by either gel electrophoresis or fluorescence polarization (FP). This technology offers a robust and cost-effective<br />
alternative to standard genotyping technologies. DNA samples from 200 individuals previously tested for the<br />
Factor V Leiden mutation and the Factor II (G20210A) mutation by PCR-RFLP were used in this study. There<br />
was complete concordance between the HAS based assay and PCR-RFLP for detecting the two mutations. We<br />
therefore conclude from our data that the HAS genotyping platform is highly accurate with advantages over other<br />
genotyping techniques in terms of cost and efficiency<br />
TP087<br />
Lilly Zhang<br />
Amgen, Inc.<br />
Pharmacokinetics and Drug Metabolism<br />
One Amgen Center Drive MS 1-1B<br />
Thousand Oaks, California 91320<br />
leiz@amgen.com<br />
189<br />
Co-Author(s)<br />
John D. Laycock, Jill Hayos,<br />
Julie Flynn, Gary Yesionek,<br />
Krys J. Miller<br />
Automated Strategies For Protein Precipitation Filtration And Solid Phase Extraction (SPE)<br />
Optimization On the TECAN Robotic Sample Processor – Applications In Quantitative<br />
LC-MS/MS Bioanalysis<br />
Strategies are discussed for developing (1) automated protein precipitation-filtration (PPF); and (2) SPE for<br />
separation of adductive metabolite from parent compound using TECAN. In part (1), the automated PPF is<br />
accomplished via the integration of TECAN with on-line positive pressure processor. Over 400 spiked plasma<br />
samples and 300 study samples underwent parallel sample preparation via PPF and manual protein precipitation<br />
with centrifugation (PPC). Sample extracts were analyzed using LC-MS/MS. Correlation between PPF and<br />
PPC was determined based on the concentration data. The results showed that PPF generated concentration<br />
results comparable to that of PPC. This enables the full aut<strong>omation</strong> of sample preparation and eliminates human<br />
intervention, peripheral equipment, and consumables needed for centrifugation. Part (2) discusses an automated<br />
strategy for SPE method development using the setup in (1). Various types of solvents and SPE beds were tested<br />
in 96-well format. The recovery of parent compound and metabolite were plotted against the SPE conditions to<br />
determine optimal combination. This fully automated SPE method selection for optimization proves thorough<br />
yet less laborious therefore has potentials in routine applications. Our strategies for addressing the integration of<br />
the robotic procedures, LC-MS/MS acquisition, and data analysis software are discussed to support automated<br />
experimental designs.<br />
POSTER ABSTRACTS
TP088<br />
Jaskiran Kaur<br />
Orochem Technologies, Inc.<br />
762 Burr Oak Drive<br />
Westmont, Illinois 60559<br />
sales@orochem.com<br />
190<br />
Co-Author(s)<br />
Asha Oroskar<br />
The OroTherm Model HC1080, Orochem Heating and Cooling Plate – A Unique Instrument<br />
Using the Latest Technology in Thermoelectric Cooling (TEC)<br />
With an overall small footprint and precise temperature control, the OroTherm 8C1080 is designed to meet the most<br />
demanding testing conditions. The cooling plate can be designed to accommodate a number of different reactor<br />
block configurations, with uniform temperature control from –10˚ C to +80˚ C to within 0.5˚ C at the control sensor.<br />
The temperature controller is configured through a personal computer using RS-232 or RS-485 bi directional<br />
control allowing the user a variety of control options. The computer set value provides for manual control of the<br />
output of either polarity, from 0% to 100% of load power. Proportional control is achieved using PID (Proportional,<br />
Integral and Derivative) control algorithms as well as dead band control (on/off) with an adjustable hysterisis may<br />
be selected. Differential temperature control is also offered when using two input sensing thermisters. Once the<br />
control parameters have configured, the PC may be removed and the controller runs as a stand-alone unit.<br />
The thermoelectric cooler used to provide the heating and cooling is designed specifically for thermal cycling<br />
applications. The unit is capable of rapid heating and cooling rates, maximum temperature of 130˚ C. The new<br />
material allow high temperature cycling of more than 600,000 cycles. This translates into a very efficient heating<br />
and cooling controller that will provide years of maintenance free service.<br />
TP089<br />
Steve Dulle<br />
Monsanto<br />
800 North<br />
St. Louis, Missouri 63167<br />
sjdull@monsanto.com<br />
Aut<strong>omation</strong> in Monsanto Biotechnology<br />
Co-Author(s)<br />
Renee Matlick<br />
Gina Balch<br />
Thomas Le<br />
Mary M. Blanchard<br />
Mark Vaudin<br />
Monsanto is a company that has transitioned from an agricultural chemical based platform to a combined<br />
chemical, plant trait and seed enhancement program. For this transition, the company has placed an emphasis on<br />
Genomics, Biotechnology, and Breeding for crop improvements and product development. Reducing costs and<br />
improving efficiencies are key business drivers for the organization. By applying a mix of biology and engineering<br />
to implement new instruments, procedures, and protocols, the Aut<strong>omation</strong> Technology team provides a seamless<br />
introduction of aut<strong>omation</strong> and biology related process improvements to the Biotech project teams. This provides<br />
value in the form of ergonomic, cost, and FTE impact.
TP090<br />
Alain Truchaud<br />
Center of Research in Biomedical Technology<br />
Institut de Biologie<br />
9 Quai Moncousu<br />
44093 Nantes Cedex 1, France<br />
a.truchaud@red-crtb.com<br />
191<br />
Co-Author(s)<br />
D. Morin, F. X. Chobletf, S. Belsoeur, T. Le Neel<br />
Center of Research in Biomedical Technology<br />
Development of an Automated Workcell Around a Multicapillary Zone Electrophoresis<br />
Instument for Human Serum Protein Analysis<br />
P. Chauvin, Sebia<br />
A new multicapillary zone electrophoresis instrument for human serum protein analysis, the Capillarys, was recently<br />
launched by Sebia company. In this instrument, serum samples are arranged manually in racks which are loaded<br />
by the technician inside the system; then, the analytical process (dilution in the buffer, injection in the capillaries,<br />
detection and data processing) is fully automated. After analysis, the rack is collected manually by the operator.<br />
We integrated the Capillarys in an automated workcell able to pick tubes from a sample transportation system<br />
(Total Laboratory Aut<strong>omation</strong>), to arrange them in the right position for barcode reading on the racks of the<br />
Capillarys. The racks are transported and loaded in the Capillarys by the robot. After analysis, racks are<br />
automatically collected and disposed on a stacker, waiting for storage, waste management, or transportation to<br />
another instrument.<br />
It was not necessary to modify neither the software, neither the hardware of the Capillarys, thus avoiding eventual<br />
conflicts about the maintenance of the instrument; we just added two external sensors for rack detection, and we<br />
modified the cover to let an easy robotic access to the rack loading and unloading area. Safety of the operator is<br />
insured by safety transparent doors equipped with sensors which stop the process if one door is opened during<br />
the process.<br />
Conclusion: we proved that the Capillarys is robot-compatible in an automated workcell, able to be connected to<br />
a Total Laboratory Aut<strong>omation</strong> system, thus introducing serum protein electrophoresis as a basic fully automated<br />
screening profile in Clinical Biochemistry, 24h/day, 7 days/week.<br />
TP091<br />
Kerstin Thurow<br />
University of Rostock<br />
Institute for Aut<strong>omation</strong><br />
R.-Wagner-Strasse 31<br />
Rostock 18119 Germany<br />
kerstin.thurow@uni-rostock.de<br />
Aut<strong>omation</strong> Systems for Bioscreening – Development and Services<br />
Co-Author(s)<br />
Kristin Entzian<br />
With the ending of important patents and the increasing resistance of micro organisms against currently available<br />
antibiotics there is a great demand of the pharmaceutical industry for the development of new leading drugs.<br />
Since the methods of combinatorial chemistry and the exploitation of new sources such as natural drugs deliver<br />
a high number of potential drugs the development of automated HTS methods for the biological testing and<br />
screening of these compounds is essential. Biological testing parameters include cell viability, cell proliferation,<br />
the measurement of intracellular calcium, the determination of enzyme inhibition or even the determination of the<br />
stability and metabolization of potential drug candidates. Different cell based and cell free assays can be used as<br />
targets for the determination of the biological activity.<br />
The presentation will give an overview about current and future developments in this field of high throughput<br />
screening. A robot based high throughput screening system was assembled and programmed for different test<br />
procedures. Examples for test results in enzyme inhibition, Ca determination and cell proliferation will be given.<br />
The flexibility of the systems enables the easy adaptation to customer specific assays and requirements and thus<br />
allows the use of the automated solution to provide screening services to pharmaceutical companies.<br />
POSTER ABSTRACTS
WP001<br />
Chris Barbagallo<br />
Millipore Corporation<br />
Research & Development<br />
17 Cherry Hill Drive<br />
Danvers, Massachusetts 01923<br />
chris_barbagallo@millipore.com<br />
Automating Large DNA Insert Preparation for Sequencing<br />
192<br />
Co-Author(s)<br />
Masaharu Mabuchi, Janet Smith,<br />
Peter Rapiejko, Joseph Hitti<br />
Bacterial Artificial Chromosome (BAC) and fosmid libraries have proven to be powerful tools for both physical<br />
mapping and the finishing process of genome sequencing. However, their unique properties (i.e., large DNA inserts)<br />
have also presented several technical challenges when adapting their preparation to higher throughput. One major<br />
obstacle to this process is the low copy number of the vectors harbored by the host cells. Typically, BAC and<br />
fosmid inserts are maintained at only 1 – 2 copies per cell. As a result, yields obtained from a given volume of<br />
culture are significantly lower than that for plasmid and cosmid DNA vectors. Modification of both bacterial growth<br />
conditions and DNA purification methodologies have been essential to maximizing yields and obtaining high quality<br />
template for downstream applications. We describe here methods that enable higher throughput processing for<br />
end sequencing of BAC and fosmid templates. Isolation of these clones and subsequent sequencing cleanup<br />
occur in a 96-well format, which is demonstrated on aut<strong>omation</strong> platforms to provide DNA of sufficient quantity<br />
and purity for direct sequencing utilizing an ABI 3700 capillary sequencer.<br />
WP002<br />
Steven Eendhuizen<br />
Spark Holland, Inc.<br />
666 Plainsboro Road, Suite 1336<br />
Plainsboro, New Jersey 08536<br />
steven.eendhuizen@sparkholland.com<br />
XLC-MS for Therapeutic Drug Monitoring<br />
Co-Author(s)<br />
Alex Berhitu, Emile Koster,<br />
Peter Ringeling, Bert Ooms<br />
Solid-phase extraction is integrated with the LC separation (“XLC”) to permit direct injection of “raw” biological<br />
samples without prior filtration, centrifugation or protein precipitation. The XLC system was coupled to MS<br />
(API2000) which is operated in positive multiple ion monitoring mode. Both thermally assisted electrospray<br />
ionization (ESI) and atmospheric pressure chemical ionization (APCI) have been considered. In order to<br />
demonstrate the XLC-MS concept for therapeutic drug monitoring (TDM) the important neuroleptic drug clozapine<br />
(CLZ), and its metabolites desmethyl-clozapine (DMC) and clozapine-N-oxide (NOX) were determined in serum.<br />
For LC-MS interfacing, detectability of CLZ and metabolites is better with the APCI interface, whereas selectivity<br />
is better with the ESI interface. Recovery is essentially quantitative (100%), detection limits are in the sub ng/<br />
mL range and the linear range is sufficiently large to cover the therapeutic range for clozapine in serum (10 to<br />
1000 ng/mL). The within day precision is < 5% (at low therapeutic levels) and the accuracy within ± 10% of the<br />
established concentrations. Optimization of SPE resulted in XLC-MS cycle times of only about 2 min. Because<br />
SPE is performed within the LC run, throughput could only be increased further by changing LC conditions, e.g.,<br />
column length, mobile phase flow rate, modifier percentage and pH. Summarizing, a sensitive, selective, robust<br />
and fast method for the monitoring of “blood levels” of clozapine and metabolites is obtained. Moreover, seamless<br />
integration of front-end sample prep and LC-MS (XLC-MS) seems to result in a universal and fully automated<br />
platform for TDM.
WP003<br />
Emily Berlin<br />
Pall Life Sciences<br />
600 South Wagner Road<br />
Ann Arbor, Michigan 48103<br />
emily_berlin@pall.com<br />
Use of the AcroPrep 96 Filter Plate for the Parallel Preparation of Recombinant Proteins<br />
193<br />
Co-Author(s)<br />
Tao Hu<br />
Kevin Seeley<br />
While genomic methods are effective for presumptive gene expression analysis, the real players in the overall<br />
control of a particular genetic trait come from any of the over 300,000 proteins found in the cell. Bottlenecks in<br />
protein production highlight the need for more effective automated systems to recover proteins for proteomic<br />
analysis. Fully automated purification strategies may include a number of steps where lysate clearance, desalting<br />
and protein concentration are needed. Beyond standard sample preparation steps, the optimization of purification<br />
for over-expressed tagged-recombinant proteins requires the screening of a collection subclones that often have<br />
highly variable expression and activity levels. Although size-separation applications can be done effectively using<br />
ultrafiltration, a more specific affinity IMAC system is typically needed to purify biomolecules from crude lysates.<br />
This study demonstrates the use of AcroPrep plates containing microporous membranes for the preparation of<br />
recombinant proteins isolated from IMAC resin minicolumns. The flexibility of the AcroPrep format is demonstrated<br />
by the creation of a matrix of IMAC conditions within a single plate. This matrix can be set up to not only help<br />
determine which metal ligand binds the target protein but can allow the design of parallel processing tests to<br />
optimize the ratio of resin to lysate for a particular clone. The construction of the AcroPrep plate allows both<br />
manual and automated handling, demonstrates bead retention, and shows consistent well-to-well performance<br />
effectively giving the protein biochemist an edge in the development in protein purification protocols.<br />
WP004<br />
Wayne Bowen<br />
TTP LabTech<br />
Melbourn Science Park<br />
Melbourn, HertsSG8 6EE United Kingdom<br />
wayne.bowen@ttplabtech.com<br />
Neutrophil Adhesion: A HTS Compatible Assay Using the Acumen Explorer<br />
Co-Author(s)<br />
John Budd<br />
A variety of pathological conditions exist in which cellular adhesion events are key to the evolving disease state.<br />
These include myocardial infarction, Adult Respiratory Distress Syndrome (ARDS), Goodpasture syndrome<br />
and general trauma. Cell adhesion also plays a crucial role in the immune system and is an important factor in<br />
inflammatory diseases such as rheumatoid arthritis, asthma, and psoriasis. The concepts involved in the cell<br />
adhesion process are a rapidly developing area of cell biology and over the last five to ten years it has become<br />
possible to work out, at a molecular level, how cells attach to each other and to extracellular matrix molecules.<br />
This will be important for future drug development. The importance of cell adhesion molecules in the homing<br />
of lymphocytes to lymphoid organs, in neutrophil localization in inflammation, and in the interaction of both<br />
lymphocytes and neutrophils with vascular endothelium suggests that defects in these molecules might have<br />
severe consequences. Initially, we have demonstrated quantification of neutrophil adhesion to confluent bovine<br />
arterial endothelial cell monolayers (BAEC) grown in 96-well plate platforms. These experiments have shown<br />
a consistent signal of 6 to 1 of PMA-stimulated neutrophils to basal adherence of unstimulated cells. These<br />
leukocyte adhesion assays, specifically adapted for use on the Acumen Explorer, could prove to be a useful<br />
adjunct for pharmacological drug screening purposes and particularly for academic research, given the speed and<br />
functionality of the Acumen Explorer technology.<br />
POSTER ABSTRACTS
WP005<br />
Carole Crittenden<br />
Molecular Devices Corporation<br />
1311 Orleans Drive<br />
Sunnyvale, California 94089<br />
Carole_Crittenden@moldev.com<br />
194<br />
Co-Author(s)<br />
Jennifer McKie<br />
Yan Zhang<br />
Oxidative Burst: Amplex ® Red Detection of Hydrogen Peroxide Release From Differentiated<br />
HL-60 Cells Using FlexStation II384 Microplate Reader and the HTS Format of FLIPR3<br />
The release of reactive oxygen species by activated human neutrophils plays an important role in the body’s<br />
defense against infection. The process of oxidative burst is correlated with many disease states, and is a primary<br />
component of phagocytosis and apoptosis in neutrophils. Here, HL-60 cells differentiated by incubation with<br />
DMSO to form neutrophil like cells are activated by phorbol 12-myristate 13-acetate (PMA) to undergo oxidative<br />
burst. In the presence of horseradish peroxidase, Amplex ® Red reagent (Molecular Probes) (10-acetyl-3, 7dihydroxyphenoxazine)<br />
is oxidized by extracellular H2O2 to produce red-fluorescent resorufin. Capabilities for<br />
reagent addition, fluorescent signal detection, and data analysis by FlexStation II384 are used to optimize a cellbased<br />
kinetic medium-throughput screen. By tuning the laser to 514.5 nm excitation and use of the 540-590nm<br />
bandpass emission filter, the assay is transferred to the high throughput screening format of FLIPR3 ® .<br />
WP006<br />
Doug Drake<br />
IDBS<br />
2 Occam Court, Surrey Research Park<br />
GuildfordGU2 7QB United Kingdom<br />
ddrake@id-bs.com<br />
Making Sense of it All: Data Management for the New Challenges in Drug Discovery<br />
Co-Author(s)<br />
Michael Collingsworth<br />
Jack Elands<br />
The dramatic increase in novel drug targets in many therapeutic areas, in conjunction with technological advances<br />
in methodology and hardware, has created an explosion of information on compounds under test for specific<br />
therapeutic targets. Increased economic pressure on drug discovery companies to “fail early” and so “fail cheaply”<br />
has resulted in parallel processing, with ADME and toxicology experimentation occurring earlier in the drug<br />
discovery process. Consequently, there is greater need for effective information management so that scientists can<br />
communicate efficiently to reach quick decisions based on a plethora of scientific data. IDBS provides software<br />
that enables discovery organizations to maximize the value of their research data. These analysis and decision<br />
making products can dramatically increase productivity and accelerate critical decision making about a candidate’s<br />
potential for success, allowing scientists to share knowledge easily across projects. The following scenario outlines<br />
a typical drug discovery scenario of creating and screening a chemical library designed to identify inhibitory activity<br />
of a MAP kinase, demonstrating how ActivityBase 5.1 can assist scientists with their data collection and analysis<br />
and help them to make informed decisions faster.
WP007<br />
Morten Egeberg<br />
Dynal Biotech ASA<br />
Molecular Systems<br />
P.O. Box 114 Smestad<br />
Oslo0309 Norway<br />
morten.egeberg@dynalbiotech.com<br />
Magnetic Bead Based High Throughput PCR- and Sequence Cleanup<br />
195<br />
Co-Author(s)<br />
Tommy Rivrud<br />
Erling Finne<br />
Dag Lillehaug<br />
The advancements in high throughput DNA sequencing technologies the last decade has led to an increased<br />
demand for efficient upstream sample preparation. Thorough removal of contaminants such as primers from<br />
the PCR reaction and un-incorporated dyes, excess nucleotides and salts from the DNA sequencing reaction<br />
combined with high yields of isolated DNA are critical to obtain high quality DNA sequences. To meet these<br />
requirements we have developed automated protocols for PCR- and sequence cleanup on Beckman Coulter’s<br />
Biomek FX liquid handling robot. These protocols are based on Dynabeads ® magnetic separation technology. We<br />
use paramagnetic Dynabeads ® to bind directly to PCR- or sequencing products. When the products are bound to<br />
the beads, a simple magnetic separation event takes place prior to washing of the beads to remove contaminants.<br />
The products are then quickly eluted from the beads for downstream handling. Compared to conventional methods<br />
such as ethanol precipitation and gel filtration, our magnetic bead based technology is ideal for implementation<br />
on automated platforms as no centrifugation or vacuum filtration steps are required. Moreover, we show that the<br />
quality of the data and the robustness of the technology meet the high requirements of today’s high throughput<br />
DNA sequencing facilities.<br />
WP008<br />
Boaz Eidelberg<br />
Bayside Motion Group<br />
Business Development<br />
27 Seaview Boulevard<br />
Port Washington, New York 11050<br />
beidelberg@baysidemotion.com<br />
Co-Author(s)<br />
Joe Timpone<br />
Guidlines for Selecting XYZ Machines for High Precision High Throughput Lab Aut<strong>omation</strong><br />
The future of high quality life longevity lies in biotechnology advances and Lab Aut<strong>omation</strong>. The immense amount<br />
of costly testing, needed to identify feasible target molecules, requires efficient Aut<strong>omation</strong> tools. Aut<strong>omation</strong><br />
is required for low precision handling of microplates, bio chips, and wells in and out anciliary Lab equipment,<br />
such as environmental cha<strong>mbers</strong> and cenrefuges. It is also required for high speed, high precision fluid handling,<br />
assaying and sensing, in a process of detecting gene expression and protein activity. The objective of this article<br />
is to discuss useful guidelines and tools for selecting optimal XYZ machines for various applications in Lab<br />
Aut<strong>omation</strong>s. Basic definitions of key process performance variables, such as accuracy, repeatability, dead band,<br />
and resolution will be made. Variables effecting process throughput such as jerk, acceleration, velocity, and settling<br />
time will be discussed. In addition, important machine variables effecting image clarity and quality of sensing, such<br />
as constant velocity and jitter will be explained. The XYZ Lab aut<strong>omation</strong> machine, is constructed as an assembly<br />
of slides, bases, bearing, encoders, motors, amplifiers, and controllers. Each element possesses numerous<br />
characteristic that effect the overall system performance. The article will highlight the relationship between key<br />
machine component parameters and the desired process performance.An analytical tool, BIMO, which assist in<br />
the selection process of an XYZ machine for Lab Aut<strong>omation</strong> process, by analyzing cost/performance tradeoffs<br />
of various options will be discussed. Finally, examples of simulated BIMO runs and actual tested data for various<br />
Lab Aut<strong>omation</strong> equipment will be presented. Bayside’s proprietary analysis tool – BIMO, will be distributed to<br />
participating audiance with interest in high precision Aut<strong>omation</strong> tools.<br />
POSTER ABSTRACTS
WP009<br />
Marcy Engelstein<br />
Millipore Corporation<br />
R & D<br />
17 Cherry Hill Drive<br />
Danvers, Massachusetts 01923<br />
marcy_engelstein@millipore.com<br />
Streamlining Aut<strong>omation</strong> of In-Gel Digestion and MALDI Target Spotting<br />
196<br />
Co-Author(s)<br />
Libby Kellard and Anja Dedeo, Millipore Corporation<br />
Mikkel Nissum, Tecan<br />
With the completion of the Human Genome Project, there is now significant emphasis on identifying proteins and<br />
protein function. As a consequence, the number of protein samples being processed has increased dramatically.<br />
Proteins are typically separated by MDLC or 2D gel electrophoresis. Those of interest are digested, concentrated/<br />
desalted, and analyzed using mass spectrometry. This procedure is time consuming and labor intensive. We<br />
describe here two methods to improve the In-Gel digestion process – a one-plate or two-plate protocol that<br />
differ primarily in their throughput and aut<strong>omation</strong> capabilities of the elution step. The one plate protocol uses the<br />
Montage ® In-Gel Digest ZP Kit (Millipore) on the Tecan Genesis ® . This procedure allows for automated processing<br />
of gel pieces in a ZipPlate micro-SPE plate up to the C18 elution step. Peptides are eluted and transferred directly<br />
to the MALDI target off-line. A fully automated process is available with this protocol using vacuum elution with<br />
subsequent spotting onto the MALDI target. The two-plate protocol utilizes a ZipPlate ® micro-SPE plate (Millipore)<br />
in tandem with a TecPro96 plate (Tecan) on the Tecan Genesis. This procedure allows for full aut<strong>omation</strong> of the<br />
entire process, including direct transfer of purified peptides from the ZipPlate plate onto a MALDI target plate for<br />
immediate analysis.<br />
WP010<br />
Xingwang Fang<br />
Ambion, Inc.<br />
Research and Development<br />
2130 Woodward Street<br />
Austin, Texas 78744<br />
xfang@ambion.com<br />
High Throughput Sample Preparation for RNAi Studies and Expression Profiling<br />
Co-Author(s)<br />
Roy C. Willis, Quoc Hoang,<br />
Michael Siano, Weiwei Xu<br />
The demand for robust high throughput RNA isolation and amplification increases very rapidly since RNAi<br />
and microarray technologies gains wide applications. We present: 1) high throughput siRNA synthesis by in<br />
vitro transcription; 2) a comparison of various high throughput RNA isolation; and 3) a fully automated mRNA<br />
amplification method for microarray analysis. In vitro transcription can generates both short (21bp) and long<br />
(>300bp) double-stranded RNAs with same function as chemical synthesized RNAs, but in much shorter time. The<br />
protocol is automatable in 96- or 384-well format, enabling quick screening for the best sequence to targeting a<br />
specific gene. Different high throughput RNA isolation methods will be compared, focusing on consistency and<br />
quality of isolated RNA and simplicity and robustness of the protocol. We’ll also discuss the streamline of RNA<br />
isolation with RNA quantification by qRT-PCR and amplification for microarray analysis. In general, microspheric<br />
bead-based approach results in more consistent RNA recovery than glass fiber filter based RNA method, and<br />
RNA can be eluted in a smaller volume. This is because beads can be fully resuspended in solution to enable<br />
more thorough mixing, washing, and elution, while the glass fiber matrix is fixed in a filter plate. In addition, we will<br />
present a high throughput sample preparation for microarray analysis. The method is based on the Van Gelder and<br />
Eberwine procedure and seamlessly integrates mRNA amplification, labeling and purification in a 96-well format.<br />
The results from beta testing of this technology will be presented.
WP011<br />
Alice Gao<br />
Corning Incorporated<br />
Corning Life Sciences-Applications<br />
2 Alfred Road<br />
Kennebunk, Maine 04043<br />
gaoa@corning.com<br />
Performance of 384 Low Volume Microplates in FP-based GPCR Binding Assays<br />
197<br />
Co-Author(s)<br />
Debra Hoover<br />
In current high throughput screening (HTS) industry, 384-well format is the most predominant form because of<br />
its reasonably high throughput as well as the availability of more robust instrumentation. However, reagent costs<br />
sometimes can make an HTS in 384-well format an unaffordable option. In this poster, We will demonstrate the<br />
use of 384 Low Volume microplates in fluorescent polarization based receptor binding assays. The total assay<br />
volume is reduced from 40 µL to 10 µL using these plates without compromising assay parameters (e.g., IC50<br />
of reference inhibitors) as well as screening parameters (e.g., Z-factor, S/N and S/B). As a result, the overall cost<br />
of screening is reduced by a factor of 4, in addition to the conservation of precious compound collections. This<br />
option also provides the advantage of avoiding the costly replacement of new instrumentation.In conclusion, we<br />
will demonstrate assay miniturization in 384-well format without compromising data quality.<br />
WP012<br />
Terry Hermann<br />
LabVantage Solutions, Inc.<br />
245 US Highway 22 West<br />
Bridgewater, New Jersey 08807<br />
thermann@labvantage.com<br />
Information and Image Management for Proteomics-based Research<br />
Co-Author(s)<br />
J. Kelly Ganjein<br />
Shariq Alavi<br />
Proteomics is one of the most important post-genomic approaches to understanding gene function and the<br />
biological processes involved in disease. Consequently, the scientific and laboratory approaches used in this<br />
avenue of discovery have generated a need for different data handling procedures. Researchers are working<br />
with solutions that force them to study data out of context, and are being asked to analyze data that has been<br />
completely dissociated from the genealogy of the original samples. The latest advances in information technology<br />
are being implemented in a solution designed to assist in properly managing the vast amount of information<br />
generated through proteomics research. We will discuss the problems resulting from the explosion of data in the<br />
proteomics field, as well as the methods used to acquire, integrate, automate, analyze, and generally manage<br />
the data. We will further consider the benefits of having an “information hub” that allows the integration of<br />
substantial quantities of highly complex and disparate data sources, thus providing a technology framework for<br />
image management. This discussion will cover key issues surrounding the design and development of a proper IT<br />
infrastructure for the life sciences, with specific relevance to the datatypes being generated in proteomics.<br />
POSTER ABSTRACTS
WP013<br />
Günther Knebel<br />
Greiner Bio-One, Inc.<br />
R & D<br />
Maybachstrasse 2<br />
Frickenhausen72636 Germany<br />
Ulrike.Honisch@gbo.com<br />
Low Birefringence Plates for Crystal Scoring Under Polarized Light<br />
198<br />
Co-Author(s)<br />
Ulrike Honisch<br />
In the post-genomic era structure determination by X-ray diffraction becomes more and more important in the<br />
context of structural genomics and structure-based drug design. Protein crystallization is still a major bottleneck in<br />
structure determination and aut<strong>omation</strong> in this area is proceeding constantly. Whereas the setup of high throughput<br />
screens for the identification of proper crystallization conditions has been subject to aut<strong>omation</strong> for some time,<br />
automated image acquisition, data analysis and crystal scoring are relatively recent projects. A powerful tool for the<br />
identification of crystals is illumination under polarized light. On the basis of its birefringent properties crystalline<br />
material can easily be distinguished from amorphous precipitate. The major obstacle in utilizing birefringence has<br />
been the birefringent background of the plastic materials used for crystallization devices. This presentation will<br />
address the current drawbacks in imaging systems with polarized light options, and salvages due to unique resins<br />
in combination with a sophisticated manufacturing process. The performance of these new non-birefringent plates<br />
will be shown in sitting drop and crystallization under oil applications.<br />
WP014<br />
David Humphries<br />
Lawrence Berkeley National Laboratory<br />
Engineering<br />
One Cyclotron Road, Mail Stop 25A-119<br />
Berkeley, California 94720<br />
DEHumphries@lbl.gov<br />
New High Performance Magnetic Separation Technology for Laboratory and<br />
Industrial Applications<br />
Co-Author(s)<br />
Martin Pollard<br />
Chris Elkin<br />
New high performance hybrid magnetic separation technology has been developed at the D.O.E. Joint Genome<br />
Institute and Lawrence Berkeley National Laboratory for general laboratory and high throughput automated<br />
applications. This technology has broad applicability for molecular separation in the areas of genomic aut<strong>omation</strong>,<br />
high throughput screening, and proteomics among others. Its applicability ranges from large and small scale<br />
microtiter plate processes and flow separation processes to single molecule DNA manipulation. It is currently<br />
an enabling purification technology for very high throughput production sequencing at the D.O.E. Joint Genome<br />
Institute. This technology incorporates hybrid magnetic structures that combine linear permanent magnet material<br />
and ferromagnetic material to produce significantly higher fields and gradients than those of currently available<br />
commercial devices. These structures incorporate ferromagnetic poles that can be easily shaped to produce<br />
complex field distributions for specialized applications. The higher maximum fields and strong gradients of the<br />
hybrid structures result in greater holding forces on magnetized targets that are being processed as well as faster<br />
extraction. Current development versions of these magnet plates have exhibited fields in excess of 9000.0 gauss<br />
and gradients approaching 1000.0 tesla/meter. The design of these structures is easily scalable to allow for field<br />
increases to significantly above 1.0 tesla (10000.0 gauss). This technology is currently being made available to<br />
industry through the Tech Transfer Department at Lawrence Berkeley National Laboratory.
WP015<br />
Diane Johnson<br />
Pfizer Global Development and Research<br />
Material Management<br />
Eastern Point Road, Box 7031-10<br />
Groton, Connecticut 06340<br />
diane_l_johnson@groton.pfizer.com<br />
199<br />
Co-Author(s)<br />
Steve Brinkman, William Heineman,<br />
Craig Hines, Michele Kelly,<br />
Kim Matus<br />
Pfizer Global Research and Development Material Management Global Center of Emphasis:<br />
Global Liquid Operations at the Groton Kings Heights Technology Center<br />
Material Management global liquid operations focuses around a two Centers of Emphasis (CoE) model; one CoE<br />
in Europe (Sandwich) and one CoE in the US (Kings Heights Technology Center, Groton Connecticut). The CoE<br />
storage and distribution model optimizes liquid compound handling access, speed, and efficiency by centralizing<br />
production functions. The Material Management-CoE model provides for redundant processing, storage and<br />
distribution capability at two sites for all of Pfizer Global Research and Development. Vital to the success of the<br />
CoE concept and global ordering was the adoption a uniform set of business rules and the deployment of a<br />
global ordering platform. Groton Material Management remains committed to providing high quality compound<br />
stewardship, storage, and processing together with corresponding data necessary to support Pfizer Global<br />
Research and Development needs.<br />
WP016<br />
Mike Jones<br />
Cambridge Antibody Technology Aut<strong>omation</strong><br />
Milstein Building, Granta Park<br />
Cambridge, Cambridgeshire CB1 6GH United Kingdom<br />
mike.jones@cambridgeantibody.com<br />
Automated DNA Sequencing: Quality Not Quantity<br />
Co-Author(s)<br />
Richard Stevens<br />
Kate Goode<br />
The DNA Chemistry facility at Cambridge Antibody Technology (CAT) is responsible for sequencing 20,000<br />
antibody fragments per month. The quality of the sequence data returned to the scientist is critical for identifying<br />
unique antibodies for further analysis. Good quality data means that the downstream process of analysing<br />
sequences is significantly reduced. Automated workstations have been set up to improve the efficiency and<br />
quality of the results, and the pass rate for first time submitted sequences is 90% or greater. Automated systems<br />
provide reproducibility through the process, along with a greatly reduced error rate than would normally be seen<br />
if the process was done manually. Flexibility, diversity, and a short process time frame have dictated the use of a<br />
workstation approach rather than a large fully automated system, with the same machine used for different parts<br />
of the process. This means that if any piece of equipment is down then the process does not come to a stand still.<br />
Here we show how we have automated DNA sequencing in the laboratory with a Tecan Genesis workstation, a<br />
Tecan Miniprep and a Perkin Elmer Evolution P3 pipettor.<br />
POSTER ABSTRACTS
WP017<br />
Lynn Jordan<br />
Zymark Corporation<br />
68 Elm Street<br />
Hopkinton, Massachusetts 01748<br />
lynn.jordan@zymark.com<br />
Aut<strong>omation</strong> of BAC 96 DNA Purification<br />
200<br />
Co-Author(s)<br />
Jim Batchelor, Kelly M. Clark, and Joseph A. Hensley<br />
Brinkmann, an Eppendorf Company<br />
Jennifer L. Halcome and George R. Halley<br />
Eppendorf - 5 Prime, Inc.<br />
Bacterial Artificial Chromosomes (BACs) play a large role in BAC-end sequencing, fingerprinting and mapping to<br />
assemble large genome constructs. In laboratories performing these applications, obtaining high quality BAC DNA<br />
in an automated high throughput manner is essential. A BAC 96 reagent kit has been performed on an automated<br />
liquid handler, utilizing a variety of accessories to achieve optimal downstream results. Resuspension, lysis, and<br />
neutralization are achieved by utilizing specialized genomic tips and an automated indexed shaker. Addition<br />
of solutions is accomplished by using a 96-tip head; and all downstream filtration steps are performed using a<br />
superior positive pressure technique, as opposed to vacuum differential. All plate movement on the deck is done<br />
using a versatile gripping system. Data will be presented to demonstrate validation of the methodology, including<br />
BAC DNA yield and purity, as well as throughput and scalability of this automated protocol.<br />
WP018<br />
Sanjaya Joshi<br />
Userspace Corporation<br />
11118 NE 141 Place<br />
Kirkland, Washington 98034<br />
sanjay@userspace.com<br />
Co-Author(s)<br />
David R. Goodlett and Hookeun Lee<br />
Institute for Systems Biology<br />
Improving the Performance of Mass Spectrometry Analysis With Automated Peak-Parking<br />
Using Off-the-shelf Components<br />
Most electrospray ionization (ESI) based Mass Spectrometry (MS) analysis algorithms prioritize high abundance<br />
pepdites in a mixture during HPLC introduction. One way to study the low abundance and co-eluting peptides<br />
in a mixture, is to slow the flow rate of the HPLC (High Performance Liquid chromatography) has to be slowed<br />
down considerably, while maintaining the gradient of the organic solvents. This technique of temporarily destroying<br />
the chromatography (in-flow) whilst studying low abundance peptides is called “Peak Parking”. This technique<br />
is specifically used to increase for tandem Mass Spectrometry (MS/MS) coverage from complex mixtures during<br />
LC-ESI-MS/MS. Userspace Corporation and the Institute for Systems Biology have been collaborating to build<br />
a modular and dynamic peak parking setup using “off-the-shelf” components. An add-on to traditional peakparking<br />
techniques is a dynamic control of the ESI voltage in Electro Spray Ionization Mass Spectrometers as<br />
flow changes. The voltage control will help maintain a stable ESI as flow rate changeswould provide an additional<br />
specificity to the entry of the peptide ions for MS/MS study. Correlation to other performance metrics variables –<br />
gradient, pump pressure, total ion count, base peak, spray voltage, MS/MS transition and flow rates – would be<br />
presented. This system is a closed-loop real-time response system which automatically identifies when peakparking<br />
needs to commence and end. Peak Parking increases the yield of peptides identified in a specific mixture.
WP019<br />
Jeff Kane<br />
Pall Life Sciences<br />
600 S. Wagner Road<br />
Ann Arbor, Michigan 48103<br />
Jeff_Kane@pall.com<br />
High Throughput Protein Recovery From Organic Solvents Using AcroPrep 96 Multi-well<br />
Plates Containing Mustang Ion Exchange Membranes<br />
201<br />
Co-Author(s)<br />
Kevin Seeley<br />
Emily Berlin<br />
While genomic methods are effective for presumptive gene expression analysis, the real players in the overall control<br />
of a particular genetic trait come from any of the over 300,000 proteins found in the cell. A majority of current<br />
separation systems use 2D-gel technologies to display differential protein patterns as well as provide a format for<br />
gel excision for the recovery, identification and detection of proteins. An alternative method to gel electrophoresis<br />
involves separating the protein population into fractions using HPLC reverse-phase chromatography. This allows<br />
the separation of proteins based on hydrophobic interactions along a first dimension but leaves the dilute protein<br />
fractions in an organic solvent. In order to process the second dimension, the protein is captured on Pall’s proprietary<br />
Mustang ion exchange membranes, eluted, concentrated with ultrafiltration (UF) and injected into a second round<br />
of HPLC or other analytical technique. This study demonstrates the use of the AcroPrep 96 Filter Plate containing<br />
Mustang membranes to develop protocols for the capture of proteins from HPLC column effluents followed by<br />
further concentration and desalting using the Acroprep 96 UF Filter Plate. The construction of the AcroPrep 96 Filter<br />
Plate allows both manual and automated handling, and shows consistent well-to-well performance effectively giving<br />
the protein biochemist an edge in the development in protein purification protocols.<br />
WP020<br />
Libby Kellard<br />
Millipore Corporation<br />
Aut<strong>omation</strong> R&D<br />
17 Cherry Hill Drive<br />
Danvers, Massachusetts 01932<br />
libby_kellard@millipore.com<br />
Co-Author(s)<br />
Sonia Gil<br />
Steven D. Sheridan<br />
Aut<strong>omation</strong> of Receptor-Ligand Binding Assays Using the MultiScreen ® HTS Filter Plate<br />
Perhaps the most critical screening parameter in the drug discovery process is the quantification of the specific<br />
affinity a drug has for a particular cellular receptor. Whether the process be carried out as a primary screening<br />
method for large compound libraries or as a secondary screening tool to rank compounds for binding affinity, high<br />
throughput, aut<strong>omation</strong> compatibility and accurate receptor-ligand binding analyses are required. The design of<br />
the new MultiScreen HTS filter plate allows the plate to be easily gripped and moved around robotic decks and<br />
associated stackers. The plate can accommodate standard bar code labeling systems for ease of tracking when<br />
processing or storing numerous samples. The MultiScreen HTS filter plate can be used for coincidence counting<br />
which provides the best signal to noise ratio. The data presented here will demonstrate aut<strong>omation</strong> of the receptor<br />
ligand binding assay using the MultiScreen HTS filter plate on the Perkin-Elmer Evolution P3 Workstation. All steps<br />
were performed using the Evolution except for incubation and the final analysis step using a scintillation counter.<br />
POSTER ABSTRACTS
WP021<br />
Dan Kephart<br />
Promega Corporation<br />
Scientific Applications<br />
2800 Woods Hollow Road<br />
Madison, Wisconsin 53711<br />
dkephart@promega.com<br />
Automated Genomic DNA Purification From Large Volumes of Whole Blood<br />
202<br />
Co-Author(s)<br />
Cris Cowan<br />
Terri Grunst<br />
We have developed a novel reagent/hardware system that enables completely automated and scaleable genomic<br />
DNA isolation from up to 10 ml of whole blood.The method takes advantage of the unique properties of Promega’s<br />
MagneSil paramagnetic particles in conjunction with a novel tube rack/magnetic device to allow isolation using<br />
standard 50ml conical tubes on a variety of automated platforms. Greater than 300µg of DNA is isolated from<br />
whole human blood containing a minimum of 1 x 10 6 white cells; over 500µg can be obtained from blood with<br />
higher white cell counts. The purification procedure is completely automated and does not require differential lysis,<br />
fractionation of white cells, centrifugation, precipitation, or rehydration of DNA prior to analysis. Performance of the<br />
isolated DNA is demonstrated in real-time and endpoint PCR, restriction digest, and STR analysis.<br />
WP022<br />
Alan Kishbaugh<br />
Meso Scale Discovery<br />
9238 Gaither Road<br />
Gaithersburg, Maryland 20877<br />
akishbaugh@meso-scale.com<br />
Co-Author(s)<br />
Gisbert Spieles, Erin Grossi, Kent Johnson,<br />
Rob Calamunci, George Sigal, Svetlana Leytner,<br />
Jacob N. Wohlstadter<br />
Multiplex Measurements of Cytokines in High Density Formats Using Multi-Array<br />
Technology<br />
This poster presents multiplexed immunoassays for human cytokines using Meso-Scale Discovery’s (MSD’s) Multi-<br />
Array platform. These assays were conducted in MSD’s new Multi-Spot plates, using a sample kit provided by<br />
MSD. The kit includes plates pre-coated with capture antibodies for each cytokine, a cocktail of labeled detection<br />
antibodies and other related assay reagents. MSD’s multiplex cytokine assays have no-wash formats, rapid<br />
protocols (2.5 hours) and fast read times (~70 sec. per plate). They are compatible with complex assay matrices<br />
(e.g., cell supernatants, cell lysates, serum, blood). No-wash assays require as little as 10 µl of sample, and<br />
have excellent selectivity (99.5%) and sensitivity (~1-10 pg/ml). The wide dynamic range (>3 logs) allows users<br />
to avoid multiple dilutions. We present data from a panel of numerous cytokines. Specifically, we demonstrate<br />
the performance of a 384-well Multi-Spot kit for measuring IL-1ß, IL-2, IL-6 and TNF-a. We also compare the<br />
performance of this kit with other MSD Multi-Spot plate formats.
WP023<br />
Gunther Kolb<br />
Institut für Mikrotechnik Mainz GmbH<br />
Chemical Process Technology<br />
Carl-Zeiss-Str. 15-20<br />
Mainz, 55129 Germany<br />
kolb@imm-mainz.de<br />
203<br />
Co-Author(s)<br />
Ines Frese, Volker Hessel, Holger Löwe,<br />
David Tiemann, and Ioan M. Ciumasu,<br />
Institut für Mikrotechnik Mainz GmbH<br />
Petra M. Krämer, TU München, Wissenschaftszentrum<br />
Weihenstephan<br />
An Automated, Portable Immunochemical Flow Injection System for On-Site Analysis of<br />
Environmentally Hazardous Chemicals<br />
The instrument presented here applies an immunochemical flow injection procedure, which uses<br />
chemiluminescence as detection principle. Analysis is performed automatically. In the final stage of the<br />
development, the operator only needs to insert the chip into the instrument, inject the sample into the chip sample<br />
reservoir, and to push the start button. The heart of the instrument is a micro-structured analysis cell made of a<br />
polymer (PMMA) which is covered with gold. On the gold surface the antibodies of the immunochemical reaction<br />
are immobilised. Both the analysis cell, a depot for the enzyme and the sample reservoir are incorporated into a<br />
removable chip. The chip is connected to the analysis instrument and may be regenerated up to 50 times after<br />
use. The analysis instrument developed by IMM is composed of a polymer plate carrying the micro-channels,<br />
valves and a step-motor driven syringe, which transports the various working media through the instrument.<br />
The measurements are temperature dependent and therefore the field version of the instrument and the liquid<br />
tanks are air-conditioned. The instrument is controlled by a low-power embedded PC via a user-friendly screen<br />
mask and touch-screen. Six – eight hours of operation independent from the power grid is possible due to two<br />
accumulators supplying the system. First tests of the portable field instrument revealed successful determination of<br />
Trinitrotoluene (TNT) standards from 0.01 to 10 µg/l. This suggests that a screening of samples for TNT is possible<br />
in this concentration range. The instrument is currently tested under field conditions on a TNT-contaminated site.<br />
WP024<br />
Kristopher Kopacz<br />
Dyax Corporation<br />
Aut<strong>omation</strong><br />
300 Technology Square<br />
Cambridge, Massachusetts 02139<br />
kkopacz@dyax.com<br />
Co-Author(s)<br />
Qi-Long Wu<br />
Janja Cosic<br />
David Buckler<br />
Multiple Parallel Purification of Fab Fragments Discovered Using Dyax Corp’s Phage Display<br />
Antibody Libraries<br />
Through phage display technology, individual lead biomolecules that bind with high affinity and specificity to<br />
specific molecular targets are selected from highly diverse phage display libraries. Various biomolecular scaffolds<br />
have been successfully used in phage display, including antibody, peptide, and protease inhibitor frameworks.<br />
In this approach for ligand discovery, combinatorial variation is introduced at specific binding regions of the<br />
display scaffold. Library me<strong>mbers</strong> with the desired binding affinity and specificity can be selected through a<br />
panning process, and the amino acid sequence of the display molecule conferring the desired properties can be<br />
determined by DNA sequencing of the isolated phage clones. After individual phage clones are identified, a key<br />
step in validating the desired binding properties for isolated clones is to express and purify the display protein<br />
to allow direct binding measurements with the target molecule. This poster will describe methods and results<br />
for high throughput soluble Fab fragment production using Dyax’s antibody discovery technology platform. Fab<br />
DNA cassettes recovered from phage clones are reformatted into Fab expression vectors to produce soluble Fab<br />
fragments at levels of 2 – 20 mg/L. These soluble Fab fragments include affinity tags that can be used for efficient<br />
multiple parallel purification.<br />
POSTER ABSTRACTS
WP025<br />
Duane Kubischta<br />
DOE Joint Genome Institute<br />
Instrumentation<br />
2800 Mitchell Drive, B100<br />
Walnut Creek, California 94598<br />
DGKubischta@lbl.gov<br />
Identifying and Reducing Crossover Contamination at the Sub-Microliter Level for Pipetting<br />
Tips in a High Throughput DNA Sequencing Environment<br />
In a high throughput sequencing environment, where hundreds of 384-well plates are processed daily, the cost<br />
and loading efficiency associated with pipetting tips necessitates that the tips are used for processing multiple<br />
plates. When these tips are re-used, despite various washing and cleaning steps, there is always a risk of crosscontamination.<br />
This type of contamination was detected on a Beckman-Coulter Biomek FX, which is used for Solid<br />
Phase Reversible Immobilization (SPRI) clean-up of labeled sequencing fragments. This paper will detail the detection<br />
and characterization of this cross-contamination and efforts that were used to reduce its occurrence.At the U.S. DOE<br />
Joint Genome Institute, a SPRI protocol is used with a magnetic bead, ethanol, and tetraethyleneglycol (BET) solution<br />
to bind ssDNA in order to clean up the remaining cellular and sequencing chemistry debris from a Rolling Circle<br />
Amplification (RCA) preparation process. This process is accomplished with a 384-well head on the Biomek FX. Plates<br />
are processed in parallel batches of 8–10 and go through the following steps: BET addition, BET incubation, BET<br />
removal on magnets, ethanol rinse on magnets, ethanol evaporation, and H20 resuspension and transfer. Pipetting<br />
tips are currently washed with deionized H20. This work was performed under the auspices of the U.S. Department<br />
of Energy’s Office of Science, Biological and Environmental Research Program and by the University of California,<br />
Lawrence Livermore National Laboratory under Contract No. W-7405-Eng-48, Lawrence Berkeley National Laboratory<br />
under contract No. DE-AC03-76SF00098 and Los Alamos National Laboratory under contract No. W-7405-ENG-36.<br />
WP026<br />
Scott Kuzdzal<br />
PerkinElmerSCIEX<br />
Proteomics<br />
710 Bridgeport Avenue<br />
Shelton, Connecticut 06484<br />
scott.kuzdzal@perkinelmer.com<br />
204<br />
Co-Author(s)<br />
Joe DiCesare, Mary Lopez,<br />
Lisa Sapp, Tillmann Ziegert<br />
Fully-Automated, High Throughput Peptide Mass Fingerprinting by MALDI Orthogonal-TOF<br />
Mass Spectrometry<br />
MALDI-TOF-MS has become an important analytical tool in the identification of proteins and evaluation of their<br />
role in biological processes. While Peptide Mass Fingerprinting (PMF) provides accurate identification of proteins,<br />
processing hundreds or thousands of samples a day can be labor and time intensive. The combination of<br />
automated liquid handlers with orthogonal MALDI-TOF provides for extremely accurate, fully-automated analysis<br />
of such samples. The prOTOF 2000 o-MALDI TOF supports 96 and 384 well stainless steel and disposable MALDI<br />
targets. These sample targets are handled by an extremely precise high-speed source (accuracy of 2 microns).<br />
Acquisition, peak-picking, protein database searching and reporting are all performed using an integrated<br />
software platform and can be automated in batch mode. The instrument can be coupled with the MultiProbe and<br />
TOFprep robotic liquid handling systems to increase sample preparation throughput. The orthogonal geometry<br />
of the instrument allows the MALDI source to be completely decoupled from the TOF chamber, eliminating any<br />
discrepancies associated with ionization and the sample target. This geometry enables the use of collisional ion<br />
cooling to focus ions from the source. As a result, the ions are equilibrated so that their energy distribution is<br />
reduced, enhancing resolution. This design eliminates the need for delayed extraction and provides enhanced<br />
mass accuracy and stability. Mass accuracies of 10 ppm or less over an entire 384 well plate are typical using a<br />
single external calibration. This instrument configuration also provides higher sensitivity and better resolution over a<br />
wider mass range. Sub-femtomole BSA digests provide sequence coverage greater than 30%.
WP027<br />
Arja Lamberg<br />
Thermo Electron<br />
Microplate Instrumentation<br />
Ratastie 2, P.O. Box 100<br />
Vantaa01620 Finland<br />
arja.lamberg@thermo.com<br />
Co-Author(s)<br />
Merja Mehto and Arja Lamberg, Thermo Electron<br />
Stine Bergholtz, David Gillooly, Diem Tran, Tine Borgen, Dynal Biotech<br />
Virpi Kymäläinen and Maija Partanen, Thermo Labsystems<br />
KingFisher 96 – A Novel High Throughput Platform for Protein Applications<br />
Sample preparation is often a limiting step for proteomic applications and requires solutions for high throughput<br />
template preparation. Magnetic particle technology provides rapid and easy to automate method for sample<br />
preparation from different sources. KingFisher family consists of three magnetic particle processors for customers<br />
having different needs for sample preparation throughput. The patented technology is based on concept where<br />
magnetic particles are transferred instead of liquids. The system is designed to automate the sample preparation<br />
process of proteins, nucleic acids and cells. We have now developed an automated method for the isolation of<br />
recombinant polyhistidine tagged proteins using KingFisher 96 and super-paramagnetic particles Dynabeads ®<br />
TALON. Dynabeads ® TALON particles employ a cobalt-based Immobilized Metal Affinity Chromatography<br />
(IMAC) on which BD TALON chemistry has been immobilized. Compared to technologies that employ nickelbased<br />
IMAC, these cobalt-based beads are able to bind polyhistidine tagged proteins with an enhanced selectivity.<br />
Using this system the isolation of 96 polyhistidine tagged proteins from bacterial lysate with high purity takes less<br />
than 25 minutes. The proteins eluted from, or bound to magnetic particles can be directly used in downstream<br />
applications such as phage display, aptamer screening or other drug discovery applications. In this poster the<br />
excellent performance of KingFisher 96 and Dynabeads ® TALON is pointed out by referring the analysis data of<br />
purified proteins.<br />
WP028<br />
Fred Lange<br />
University of Rostock<br />
Institute of Aut<strong>omation</strong><br />
R.-Wagner-Str. 31<br />
Rostock18119 Germany<br />
fred.lange@uni-rostock.de<br />
205<br />
Co-Author(s)<br />
Regina Stoll<br />
Thomas Renger<br />
High Resolution Mobile Measurement of Oxygen Concentration in Main Breath Stream by<br />
Optical Oxygen Sensing – An Application in Occupational and Sports Medicine<br />
Measurement of cardiocirculatory parameters like heart rate and blood pressure does not bring sufficient results<br />
for evaluation of metabolic components of physical behavior of the organism especially regarding strain under<br />
labour and different intensities of sport’s activities. So, new ways for miniaturizing and optimizing of gas sensors<br />
are important. The development result shows an application of a miniature, high resolution fast optical fluorescence<br />
based measurement system positioned in the breath main stream. In a first step the sensor is inplemented into a<br />
mask, in further developments a “headset ensemble” is the goal.The system can be used for functional and fitness<br />
determination in the sport’s medical diagnostics lab as well as in the field or in labour environments. A special<br />
application is performed to be used as a physiological monitoring system in space.<br />
POSTER ABSTRACTS
WP030<br />
Kurt Lund<br />
ACESystems, Inc.<br />
135 Sixth Street<br />
Del Mar, California 92014<br />
klund@abac.com<br />
The HTS Compound-Thawing Bottle Neck (and How to Avoid it With a New Processor)<br />
In terms of laboratory HTS efficiency, the thawing requirement for stored compounds presents itself as a huge<br />
bottleneck…until now with the advent of the new Thawing Processor System (TPS) from ACESystems, Inc. In the<br />
present paper the thawing behavior of solvents frozen in microplates is investigated in two types of experiments:<br />
(1) conventional thawing on the bench, and (2) rapid thawing with the TPS. It is also discussed how the TPS<br />
allows wells to be thawed, individually without disturbing the other wells in the plate, thus preserving precious<br />
compounds. The clearest observation from the bench tests (1) is that solvents in the wells remain largely frozen<br />
for hours after plate-removal from the freezer. Thus, for a center well of a 96-well microplate, even 3 hours was<br />
not enough time to effect 100% thawing on the bench. The rapid thawing experiment (2) was conducted using<br />
computer feedback control with the TPS. For DMSO in a standard one-ml well, frozen to -5˚ C, this resulted in<br />
100% thawing in only 31⁄2 minutes, without causing excessive temperatures to the solvent. Overall, it is concluded<br />
that there can be great enhancement to laboratory efficiency with the Thawing Processor System, as well as large<br />
savings in compound preservation by thawing only selected wells in a microplate, instead of the whole plate.<br />
WP031<br />
Joseph Machamer<br />
Molecular Devices<br />
1311 Orleans Drive<br />
Sunnyvale, California 94089-1136<br />
joe_machamer@moldev.com<br />
206<br />
Co-Author(s)<br />
Greg Kazan,<br />
Molecular Devices<br />
Tom Onofrey, Millipore Corp.<br />
Recent Developments in High Throughput, Turn-Key Solubility, and Permeability Compound<br />
Ranking Assays<br />
When combined with data from primary screening assays, measurement of the physiochemical properties of new<br />
chemical entities can provide a basis for the ranking of compounds based on their potential to be developed<br />
into viable drug candidates. The strategy of ranking the developability of new chemical entities (NCE) based on<br />
characterization by high throughput characterization of compound solubility and permeability is increasingly being<br />
applied in the drug discovery industry. We have developed automatable, turn-key assays to measure solubility and<br />
permeability that use Millipore membrane technology and a Molecular Devices SpectraMax absorbance microplate<br />
reader. The benefits of this approach include good correlation with low throughput, “gold standard” assays, no<br />
methods development, aut<strong>omation</strong> friendliness, reduced need for instrument expertise, and software flagging of<br />
data validity.
WP032<br />
Colin Masui<br />
Symyx Technologies<br />
Life Sciences<br />
3100 Central Expressway<br />
Santa Clara, California 95051<br />
cmasui@symyx.com<br />
High Throughput Process Optimization for the Fine Chemical and Pharmaceutical Industries<br />
Symyx Technologies is currently applying its expertise in high throughput screening to develop fully integrated<br />
workflows for process optimization that greatly reduces the time and material needed to find the optimum<br />
conditions for a given organic transformation. Automated robotics is utilized in order to prepare catalysts and<br />
substrates and for post reaction workup and analytical preparation. The ability to run under a diverse set of<br />
conditions with temperature ranges from -20 to 200˚C and pressures up to 1500psi are capable using proprietary<br />
reactor formats (96 well Hip and PPR ® -48 reactors). Automated in situ injection of catalysts and substrates under<br />
reaction pressure is capable in the Parallel Pressure Reactor (PPR ® -48) System. The entire systems are completed<br />
by incorporation of the Symyx Renaissance software suite which delivers a complete, secure database<br />
environment to design experiments, control robotics, capture, store, recover, and explore the data and information<br />
from every stage of the workflow.<br />
WP033<br />
Timothy McCauley<br />
Pierce Biotechnology, Inc.<br />
R&D<br />
3747 N. Meridian Road<br />
Rockford, Illinois 61101<br />
tim.mccauley@piercenet.com<br />
207<br />
Co-Author(s)<br />
Mahesh Mathrubutham, Sherri Z. Millis,<br />
Aric G. Morgan, Michael L. Stanaitis<br />
IQ ® Technology: An Automated HTS Assay for Screening Kinase, Phosphatase, and<br />
Protease Targets<br />
IQ ® Technology, a patent-pending homogeneous, universal detection platform originally designed for high throughput<br />
screening of kinases and phosphatases, has now been applied to screening of proteases. Representative enzymes<br />
from each of the major classes of proteases have been assayed in the IQ format. Enzyme activity and compound<br />
inhibition data will be presented for such enzymes as Trypsin, MMP-3 and Caspase-3. The technology has been<br />
tested in 96- to 384- to 1536-well microplate formats and is universally suited for automated screening systems.<br />
IQ Technology is a direct, non-competitive assay format that does not require antibodies or radioactive reagents.<br />
Fluorophore-labeled peptides are used as enzyme substrates. Kinase or phosphatase activity is quantified by direct<br />
measurement of the phosphorylation state of the substrate. For protease activity, cleavage is quantified by utilizing<br />
a peptide substrate containing a phospho-residue distal to the fluorphore. Protease assays result in cleavage of the<br />
substrate, which liberates the fluorphore-labeled terminus from the phosphorylated terminus. Cleavage is measured<br />
by the change in fluorescence intensity that occurs when a proprietary iron-containing compound binds specifically<br />
to phosphoryl groups on peptides and quenches the fluorescence. Ki’s generated using this platform correlate with<br />
published values. IQ Technology provides a universal method that can be used with any peptide sequence and<br />
is insensitive to high concentrations of ATP and substrate. The IQ Technology has been validated against a large<br />
number of detergents, organics, and other reagents found in reaction mixtures and has been optimized for high<br />
throughput applications with representative Z´ values of 0.7.<br />
POSTER ABSTRACTS
WP034<br />
Ruth H. Myers<br />
Aurora Instruments, LLC<br />
Instrumentation & Customer Solutions<br />
9645 Scranton Road, Suite 140<br />
San Diego, California 92121<br />
ruth_myers@aurorainstruments.com<br />
208<br />
Co-Author(s)<br />
Jason Johnson<br />
Chris Biagioli<br />
Solving the Dispensing Challenges of Assay Miniaturization in High-Density Microplates<br />
As the pharmaceutical industry rapidly progresses towards greater utilization of high-density microplates,<br />
instrumentation technology struggles with the challenges imposed by assay miniaturization. Aurora Instruments<br />
has overcome these challenges by employing the technical foundation established in the development of the<br />
Ultra High Throughput Screening System (UHTSS) platform. This poster will describe the fluidic challenges<br />
that miniaturization technology strives to surmount and the strategies that Aurora Instruments employs in the<br />
development of their dispensing technology. Miniaturization technology faces formidable challenges to achieve<br />
higher throughput, while lowering costs, maintaining high quality data, and delivering it all in a smaller instrument<br />
package. With the implementation of high-density microplates, the ability to control fluidics in terms of nanoliter<br />
volumes becomes a necessity. Through novel design, Aurora Instruments surmounts the hurdles of dispensing<br />
with the Flying Reagent Dispenser (FRD). In order to maintain high quality data, dispensing technology must be<br />
capable of precise dispensing of nanoliter volumes without cross-contamination. Aurora’s FRD uses non-contact<br />
dispensing and four independent fluid pathways to address 384-well, 1536-well, and 3456-well microplates across<br />
a volume range of 200 nL to 9 µL. The FRD supports both fully integrated high throughput screening and standalone<br />
assay development applications.<br />
WP035<br />
Pankaj Oberoi<br />
Meso Scale Discovery<br />
9238 Gaither Road<br />
Gaithersburg, Maryland 20877<br />
poberoi@meso-scale.com<br />
Ultra-High Throughput Screening Using Multi-Array 1536-Well Plates<br />
Co-Author(s)<br />
David Stewart, Kevin Khovananth, Stephen Tessier,<br />
Alan Kishbaugh, Kent Johnson, Jacob Wohlstadter<br />
Meso Scale Discovery has expanded its technology platform for detection of biomolecules and other analytes<br />
to include Multi-Array 1536-well plates. These plates allow for experimentation and screening in ultra high<br />
throughput modes with a per plate read time of approximately one minute. Similar to other MSD Multi-Array<br />
plates, the 1536-well plates are available with different surface properties, have high sensitivity (100 attomoles),<br />
have a wide dynamic range (5 logs) with no significant well-to-well cross-talk, and are compatible with most lab<br />
aut<strong>omation</strong> equipment. Assays developed on 384-well Multi-Array plates were transferred to 1536-well plates<br />
for a number of different assays including biotin-avidin, protein-protein, and receptor-ligand binding systems. We<br />
present screening data with workflows capable of greater than 500,000 data points per shift.
WP036<br />
Jeff Olson<br />
Abbott Laboratories<br />
R46S<br />
200 Abbott Park Road<br />
Abbott Park, Illinois 60064-6212<br />
jeff.olson@abbott.com<br />
Membrane-Bound Protein Crystallization Workstation<br />
209<br />
Co-Author(s)<br />
Mark Chiu<br />
Mike McCoy<br />
Jeff Pan<br />
This poster describes a recently developed method for dispensing protein-containing, gel-like, lipidic cubic phases<br />
onto crystallization trays, and preparation of these trays for various protein crystallization experiments. Tiny<br />
volumes (typically 200 nL – 1 µL) of lipidic cubic phase material containing either soluble or membrane associated<br />
proteins are deposited onto multi-well trays for crystallization experiments which are prepared using either free<br />
interface diffusion, sitting drop, or batch methodologies. The fundamental principle involves: (1) the preparation of<br />
the protein containing gel and loading into a novel, gel dispensing device; (2) positive-displacement dispensing of<br />
the protein containing gel onto the assay trays; (3) preparation of the trays for various experiments by automated<br />
addition of precipitating buffers or other reagents, and (4) application of humidity control to reduce concentration<br />
changes of the reagents due to evaporation. The system, which is based on a standard Gilson 215 pipetting<br />
robot, is fully automated and enables a far greater number of membrane-protein crystallization experiments to be<br />
performed than could be accomplished manually.<br />
WP037<br />
Cengiz S. Ozkan<br />
University of California, Riverside<br />
Mechanical Engineering<br />
Bourns Hall, A305<br />
Riverside, California 92521<br />
cozkan@engr.ucr.edu<br />
Cell Based Biosensors<br />
Cell based biosensors offer the capability for detecting chemical and biological agents in a wide spectrum.<br />
Membrane excitability in cells plays a key role in modulating the electrical activity due to chemical agents.<br />
However, the complexity of these signals makes the interpretation of the cellular response to a chemical agent<br />
rather difficult. It is possible to determine a frequency spectrum also known as the signature pattern vector<br />
(SPV) for a given chemical agent through analysis of the power spectrum of the cell signal. It is also essential to<br />
characterize single cell sensitivity and response time for specific chemical agents for developing detect-to-warn<br />
biosensors. In order to determine the real time sensing capability of single cell based sensors, multi-chemical<br />
or cascaded sensing is conducted and the performance of the sensor is evaluated. We describe a system for<br />
the measurement of extracellular potentials from primary rat osteoblast cells isolated onto planar microelectrode<br />
arrays using a gradient AC electric field. Fast Fourier and Wavelet Transformation techniques are used to extract<br />
information related to the frequency of firing from the extracellular potential. Quantitative dose response curves<br />
and response times are obtained using local time domain characterization techniques. Future applications of this<br />
technique will also be discussed.<br />
POSTER ABSTRACTS
WP038<br />
Laura Pajak<br />
Beckman Coulter, Inc.<br />
Biomedical Research Division<br />
7451 Winton Drive<br />
Indianapolis, Indiana 46268<br />
laura.pajak@sagian.com<br />
Using Beckman Coulter’s Biomek ® NX Laboratory Aut<strong>omation</strong> Workstation for the<br />
Purification of High Quality Plasmid DNA<br />
210<br />
Co-Author(s)<br />
Chad Pittman<br />
Scott Boyer<br />
The information included in this poster describes the utilization of a new automated liquid handler, the Biomek NX<br />
Laboratory Aut<strong>omation</strong> Workstation, in the preparation of plasmid DNA using Promega’s Wizard ® SV 96 Plasmid<br />
DNA Purification System. A single plate of bacterial pellets can be purified on the deck of the Biomek NX in<br />
approximately 50 minutes. The following system components, for the purification of plasmids, will be described:<br />
• The hardware requirements for the Biomek NX<br />
• Software and method to drive the aut<strong>omation</strong> workstation<br />
• Results when purifying plasmids using Wizard SV96 reagents.<br />
Plasmids were analyzed by standard methodologies to assess the quality and quantity of product. Automated<br />
capillary sequencing on Beckman Coulter’s CEQ 8000 Genetic Analysis System was performed thereby<br />
demonstrating the suitability of the purified plasmids for capillary sequencing.<br />
WP039<br />
Geoff Parker<br />
Scimcom<br />
NewMarket Road, Fordham<br />
Cambs CB7 5WW United Kingdom<br />
gparker@scimcom.com<br />
Right Time, Right Place – Right Information?<br />
It has been estimated that two out of every three business decisions are the wrong decisions. These wrong<br />
decisions usually happen because people do not have the right information, and the right amount of information<br />
they need to make a more informed, and therefore more appropriate decision. The amount of information<br />
produced by and held in all businesses is exploding. There is simply more information available than people can<br />
process. Competition, regulation and computerisation have all played a part in producing a mountain of information<br />
that is often difficult to view and access, and sometimes near impossible to gain real value from.The sheer volume<br />
of information detracts from its worth. Organizations are unable to extract the knowledge or data they really need,<br />
and so the mass is rendered valueless. With so much new information rapidly adding to the top of the pile, the life<br />
value of knowledge is decreasing. Quite simply, the longer you have data the older and less valuable it becomes.<br />
So companies need to harness their data as rapidly as possible, and get the right (amount of) information to the<br />
right place at the right time. This paper will address how organizations can climb, conquer, and control the data<br />
mountain. It will consider approaches that companies can adopt to realise the sustainable competitive advantage<br />
that is locked in their knowledge, information, data, and resources.
WP040<br />
Fiona Payne<br />
Zinsser Analytic GmbH<br />
Eschborner Landstrasse 135<br />
FrankfurtD-60489 Germany<br />
info@zinsser-analytic.com<br />
Aut<strong>omation</strong> Platform for Salt Prescreening and Polymorph Screening Studies<br />
211<br />
Co-Author(s)<br />
Werner Zinsser<br />
Polymorphism has become a concern in today’s pharmaceutical research labs as it affects a number of issues<br />
in pharmaceutical systems varying from processing characteristics to bioavailability. During thr pre-formulation<br />
stage of drug development, polymorph screening is a tedious and time-consuming process. Zinsser Analytic<br />
has developed Crissy, an automated liquid and powder handling platform for salt pre-screening and polymorph<br />
screening studies. This platform automates the necessary steps for extensive crystallisation using different<br />
solvents, temperatures, concentrations, agitation and pH-measurement. A special Crissy reactor block has been<br />
designed to directly present the crystals to the XRPD system without any additional sampling steps. The top of<br />
the reactor bottoms is ideal for XRPD-detection. Cross contamination caused by electrostatic force moving the<br />
powders, is minimized as the detector block itself is grounded and the individual reactor cavities are separated in<br />
from each other the “up”-position.<br />
WP041<br />
Jonathan Petersen<br />
Molecular Devices Corp.<br />
Instrument R&D<br />
1311 Orleans Drive<br />
Sunnyvale, California 94089<br />
jon_petersen@moldev.com<br />
Co-Author(s)<br />
AquaMax DW4: A Flexible Tool for Heterogeneous 1536 Assays<br />
Co-Author(s)<br />
Jeannie Nguyen<br />
Annegret Boge<br />
Gayle Teixeira<br />
The AquaMax DW4 is a new liquid handling instrument for assembly of screening assays in 96, 384 and 1536<br />
formats. Because it can dispense up to four different assay components, the AquaMax can assemble assays of<br />
varying complexity. It is also a plate washer, thus enabling heterogeneous assays such as ELISAs. The AquaMax<br />
can dispense volumes as small as 0.5 µL to enable miniaturized assays, or up to 320 µL. The AquaMax has high<br />
throughput, making it suitable for screening applications. It can be used to dispense liquid reagents as well as<br />
suspensions of beads or cells. Both aspiration and dispensing are gentle enough for cellular assays that require<br />
washing. This combination of performance and flexibility makes the AquaMax Liquid Handler a powerful tool<br />
for assembling assays for drug discovery. The poster will present basic performance data for the dispenser and<br />
washer. It will also demonstrate the instrument’s utility in assembling homogeneous, heterogeneous, and cellular<br />
assays.<br />
POSTER ABSTRACTS
WP042<br />
Chad Pittman<br />
Beckman Coulter, Inc.<br />
Applications<br />
7451 Winton Drive<br />
Indianapolis, Indiana 46268<br />
chad.pittman@sagian.com<br />
212<br />
Co-Author(s)<br />
Laura Pajak<br />
Scott Boyer<br />
Aut<strong>omation</strong> of Immunotech’s IL-8 ELISA Using Beckman Coulter’s Biomek 3000 Laboratory<br />
Aut<strong>omation</strong> Workstation<br />
Beckman Coulter, has developed and optimized an automated method using the new Biomek 3000 Laboratory<br />
Automated Workstation to provide a complete, walk away system for ELISA assays. The system uses the unified<br />
Biomek software to optimize liquid transfers while making sample processing as efficient as possible. The method<br />
allows processing of a single plate while minimizing reagent waste. Integrated plate washing is achieved using<br />
the Wash–8 Tool that provides consistent reagent addition and removal. The combination of the Biomek 3000<br />
Laboratory Automated Workstation and integrated plate washing offers a complete system for processing ELISA<br />
assays without user intervention. The information provided here will:<br />
• describe the automated system used to process the ELISA;<br />
• demonstrate the utility of the Biomek 3000;<br />
• describe the results when processing Immunotech’s IL-8ELISA kit on this system.<br />
WP043<br />
Ileana Place<br />
STEM/ReactArray<br />
2555 Kerper Boulevard<br />
Dubuque, Iowa 52001<br />
ibplace@branstead.com<br />
STEM ReactArray Improved Productivity Using Manual and Automated Parallel Reactors<br />
Equipped With Liquid Handling and On-line HPLC Analysis<br />
ReactArray was developed by an on-going collaboration between the leading drug companies and the instrument<br />
industry and is ideal for large range of uses, like process chemistry, drug degradation studies, formulation<br />
applications and crystallization studies. ReactArray automates reaction preparation, sampling, quenching, dilution,<br />
and HPLC analysis and provides the advantage of monitoring reactions under precisely controlled conditions.
WP044<br />
Dieter Popp<br />
Tecan Austria GmbH<br />
Untersbergstr. 1A<br />
Groedig/SalzburgA-5082<br />
dieter.popp@tecan.com<br />
213<br />
Co-Author(s)<br />
Mateja Niederreiter, Manfred Lansing,<br />
David Yost, Klaus Döring,<br />
Melissa Foshee<br />
Characterization of Human Alpha-Thrombin Aptamer System by Automated Fluorescence<br />
Lifetime Analysis<br />
One particular problem hampering the high throughput drug screening process today, is auto-fluorescence of<br />
new chemical entities (NCEs). Many fluorescence based measurements can be significantly affected by these<br />
auto-fluorescent NCEs, resulting in a decrease in assay specificity and an increase in the false positive rate.<br />
This obviously affects both throughput and cost during a screening campaign. Fluorescence Lifetime (FLT)<br />
measurements appear to be exempt from this type of interference, thus virtually eliminating false positive results<br />
due to auto-fluorescence of NCEs. Tecan has recently developed and launched a microplate reader to detect<br />
Fluorescence Lifetime signals. To demonstrate the usefulness and robustness of the FLT detection technology a<br />
model assay was developed using aptamers. Aptamers are single-stranded nucleic acid oligomers, that analogous<br />
to antibodies are capable of binding target molecules with high affinity and specificity. Employing their unique<br />
binding properties, aptamers can be used as a screening tool for identification of unique ligands, i.e., new drug<br />
candidates in drug discovery. The human alpha-thrombin aptamer is a DNA 15-mer that binds to thrombin and<br />
inhibits its proteolytic activity during the blood clotting cascade. The specific interaction was monitored by the<br />
fluorescence lifetime change of a fluorescence label conjugated to the thrombin aptamer. In order to illustrate<br />
the potential for drug discovery, we have used the thrombin inhibitor hirudin and studied its effect on aptamerthrombin<br />
complex formation. All FLT measurements were performed on Tecan’s ULTRA Evolution reader.<br />
WP045<br />
Johannes Posch<br />
Tecan Austria GmbH<br />
Marketing<br />
Untersbergstrasse 1A<br />
GroedigA-5082 Austria<br />
johannes.posch@tecan.com<br />
Tecan’s LSx00: Automated Processing and Analysis of Microarrays Implemented Prior<br />
Content Quality Control<br />
Co-Author(s)<br />
Ralph Beneke<br />
Gerald Probst<br />
Homogeneous high signal to background ratios and scan-to-scan reproducibility of microarray are desirable<br />
to increase reliability of conclusions drawn from them. Aut<strong>omation</strong> of process from sample preparation and<br />
processing to data acquisition and analysis is an essential requirement to reduce experimental variation as well<br />
as increase number of experimental repetitions possible – one of the major bottlenecks regarding statistical<br />
probability. Tecan’s LSx00 series scanner in combination with MediaCybernetics’ ArrayPro4.5 offers the ultimate<br />
solution for high reproducibility of the scanning and analysis process combined with outstanding flexibility. The<br />
LSx00 series scanner is the breakthrough and enabling solution for Academia as well as Biotech in R&D and high<br />
throughput facilities by improving reproducibility and safety of microarray experiments. We present fully automated<br />
batch run of classical slide arrays with fluorescence independent pre-check of spot quality on slides prior to<br />
hybridization – and therefore without using and bleaching dyes. A QC of spots and slides prior to hybridization<br />
saves money and enables troubleshooting of “bad spots” before and after slide processing.<br />
POSTER ABSTRACTS
WP046<br />
Lynn Rasmussen<br />
SAIC: NCI Frederick<br />
Molecular Technology<br />
915 Tollhouse Avenue, Suite 211<br />
Frederick, Maryland 21702<br />
rasmussn@mail.ncifcrf.gov<br />
High Throughput Peptide Synthesis on a Biomek FX Liquidhandler<br />
214<br />
Co-Author(s)<br />
Carl Saxinger, Casey Frankenberger, David Munroe,<br />
SAIC: NCI Frederick<br />
Marc Goldstein, Beckman-Coulter<br />
Protein and peptide microarrays are being developed as tools for proteomics. The sutibility of a peptide for use<br />
in the microarray format must be determined empirically. Current methodologies for peptide synthesis, while<br />
automated, are not high throughput. To produce thousands of peptides for testing is slow and expensive. We have<br />
developed a simple method to produce small quantities of large nu<strong>mbers</strong> of peptides for screening. Once peptides<br />
are selected as microarray compatible, conventional methods are used to produce the quantities necessary for<br />
printing.<br />
WP047<br />
Kirby Reed<br />
Gilson, Inc.<br />
Applications<br />
3000 West Beltline Highway<br />
Middleton, Wisconsin 53562<br />
kreed@gilson.com<br />
Novel Approach for Screening of Drug Absorption Via an Automated System<br />
Co-Author(s)<br />
Alan Hamstra<br />
It is imperative in drug discovery and ADMET to have a handle on the membrane permeability of the drug<br />
compounds and small molecules. Membrane permeability in turn predicts the clinical absorption of these<br />
compounds/drugs. Acceptable methods for estimating drug permeability include liposome assays, Caco-2 cell line<br />
culture or intestinal tissue. A basic issue with these methods lies in the fact that they can be costly and extremely<br />
labor intense. An alternative to those methods involves Immobilized Artificial Membrane (IAM) chromatography<br />
phases, which mimic the lipid environment found in cell membranes. IAM fast-screen mini columns (1 cm x 3 mm)<br />
determine drug capacity factor (k´IAM), which correlates well with drug permeability in Caco-2 cells. Implementing<br />
the IAM fast-screen columns in a HTS lab to determine drug permeability would be extremely advantageous,<br />
allowing for the screening of 100’s to 1000’s of possible drug candidates in a fraction of the time, at substantially<br />
reduced cost. The automated system has the capabilities of incorporating 1-4 HPLC systems to increase the<br />
throughput for the analysis. The systems run independent of one another which is extremely important since the<br />
chromatographic retention factor k´ is measured, and cannot be affected by flow splitting. The system also is<br />
capable of solubilizing the dry compounds prior to injection. The system can transfer the “hits” to a “hit” plate for<br />
further analysis or storage.
WP048<br />
Scott Reeves<br />
REMP USA<br />
150 Hopping Brook Road<br />
Holliston, Massachusetts 01746<br />
scott.reeves@remp.com<br />
Co-Author(s)<br />
Michael Girardi, REMP USA<br />
Carol Homon, Boehringer Ingelheim Pharmaceuticals, Inc.<br />
Thorsten Poetter, Bayer Crop Science AG<br />
Berta Strulovici, Merck & Company, Inc.<br />
Gerhard Mihm, Boehringer-Ingelheim Pharma KG<br />
Tools for Sample Management that are Efficient, Versatile, Proven and Evolving – REMP Tube<br />
Technologies<br />
High Throughput Screening (HTS) has been heralded as the way to develop better drugs faster. Screening<br />
technologies in drug discovery have recently seen dramatic advances which have led to throughputs of<br />
over 100,000 compounds per assay per day. There has also been an emergence of a plethora of new assay<br />
technologies enabling a greater range of targets to be screened, all of which have helped catapult HTS into ultra<br />
HTS (UHTS).<br />
However, to be successful at drug discovery, the Compound Management operation must be efficient, versatile,<br />
and evolving while trying to ensure that their compound libraries are of the highest quality. In the past, the<br />
management of compound collections were performed by groups that were viewed as serving simple dispensary<br />
functions. Investment in this operation was small and its role not understood by many in drug discovery<br />
organizations. The importance of the corporate compound collection, often regarded as “the company’s largest<br />
asset” or it’s “crown jewels,” shifted from the collection size being the most important factor to a more balanced<br />
view of the importance of quality and quantity. The results of HTS and UHTS, as well as the drug discovery<br />
process as a whole, is only as good as the compounds that are delivered and, therefore, a very important factor in<br />
its relative success or failure. This poster will provide some insight and data from several Compound Management<br />
operations that are considered to be efficient, versatile and evolving and describe how this has been achieved.<br />
WP049<br />
Heidrun Rhode<br />
Friedrich-Schiller-University, Medical Faculty<br />
Institute of Biochemistry<br />
Jena07740 Germany<br />
heidrun-rhode@mti.uni-jena.de<br />
Co-Author(s)<br />
M. Schulze, G. A. Cumme, A. Horn, Simon Renard, and<br />
Thomas Moore, Friedrich-Schiller-University, Medical Faculty<br />
Peter Zimmermann, CyBio AG<br />
Multichannel HTS/ µHTS Liquid Handling Systems – Check Using Two-Indicator Systems<br />
Multichannel HTS/µHTS liquid handling systems have to be checked under conditions close to the screening<br />
reality, i.e., with commonly used sample solutions and employing microplate technology. We propose a simple<br />
method to determine precision and accuracy of multichannel liquid handling systems using a combination of<br />
gravimetric and optical measurements. The precision of the optical signal is improved by multi-wavelength<br />
measurements using two indicators and buffers with low temperature dependence of pH. Factors causing nonlinearity<br />
between the sample volume delivered into a well and the optical signal are considered. The resolution<br />
of the method i.e., the imprecision of the measuring system as determined by the imprecision of the signal of a<br />
homogeneous solution with two indicators is better than 0.3% CV and 2% CV using absorbance and fluorescence<br />
measurements, respectively, suggesting that absorbance measurements should be preferred. The gravimetrically<br />
determined inaccuracy is less than 0.5% CV. The suitability of the method proposed is demonstrated with two<br />
liquid handling devices using different ejection principles, with commonly used fluids, with 96, 384 and 1536<br />
microplates, and with photometric and fluorimetric indicators. Despite the substantial improvement of fluorescence<br />
signals by multi-wavelength measurements, fluorimetry still tends to overestimate the imprecision of a liquid<br />
handling system. Imprecisions obtained with optimized methods and with both devices were lower than 2% CV for<br />
2 µl set volume with 384- and 1536-well microplates and about 1% CV with higher set volumes.<br />
215<br />
POSTER ABSTRACTS
WP050<br />
Steve Richmond<br />
Genetix Ltd<br />
R&D<br />
Queensway<br />
New MiltonBH25 5NN United Kingdom<br />
steve.richmond@genetix.com<br />
AliQuot – High Throughput Liquid Dispensing Into Microplates<br />
216<br />
Co-Author(s)<br />
Sky Jiang, Paul Raine, Sarah Stephens,<br />
Chris Mann, Julian F. Burke<br />
There is a need for an automated, high throughput system capable of dispensing low volumes into high density<br />
microplates accurately and precisely. At Genetix we have developed the aliQuot. This is a small footprint, bench<br />
top machine that can dispense volumes in the range 1 – 350 µl into 96-, 384- and 1536-well microplates with a<br />
CV of 3 % at 10 µl. The system has been designed to minimise the dead volume and there is a backflush facility<br />
to recover liquid at the end of a run, thus minimising loss of precious samples. To allow extended unattended run<br />
times, a stacker system that holds 40 microplates is available and the machine incorporates a RS232 interface to<br />
enable remote operation. In this poster we demonstrate the accuracy and precision of the aliQuot and its use for<br />
high throughput applications.<br />
WP051<br />
Luke Roenneburg<br />
Gilson, Inc.<br />
Applications<br />
3000 West Beltline Highway<br />
Middleton, Wisconsin 53562<br />
lroenneburg@gilson.com<br />
A Totally Automated Solution for Normal and RP Preparative HPLC With Analytical<br />
Purification Determination: cLC<br />
Co-Author(s)<br />
Alan Hamstra<br />
It’s imperative in today’s world that researchers get the most bang for their buck. Purification of compounds has<br />
largely been accomplished through liquid-based chromatography. The advantages of preparative chromatography for<br />
compound purification are well established. Reverse phase chromatography, although being the method of choice,<br />
is limited by sample capacity /injection, possible sample solubility issues and lengthy dry downs. It would be very<br />
advantageous to researchers if one could expand the capabilities of these instruments. Researchers have shown<br />
quite an interest in implementing normal phase chromatography for compound purification. Benefits such as fast dry<br />
downs and enhanced solubility make normal phase chromatography an attractive alternative for compounds that<br />
are incompatible with RP chromatography. A possible solution to this dilemma is to automate the capabilities of RP<br />
and NP in one system in addition to being able to analyze the collected fraction for purity. The cLC (comprehensive<br />
LC ) system is a totally automated purification system with on-line analytical analysis of fractions, using both NP<br />
and RP environments. The cLC system requires only a little more bench space than a conventional HPLC system<br />
at about the same price. The system can automatically switch between normal and reverse phase, and is capable<br />
of accessing both pre-packed disposable silica-based columns and reverse phase columns without operator<br />
intervention. Collected fractions can then automatically be injected for determination of purity. Presentation of the<br />
system will include data representing the throughput and analysis capabilities of the system.
WP052<br />
Jas Sanghera<br />
TTP LabTech<br />
Melbourn Science Park, Cambridge Road<br />
Royston SG8 6EE United Kingdom<br />
jas.sanghera@ttplabtech.com<br />
217<br />
Co-Author(s)<br />
David Pole<br />
Wayne Bowen<br />
A Homogenous Assay for Inhibition of Basal Proliferation in 96- and 384-Well Formats Using<br />
the Acumen Explorer<br />
Estimating for a given cell population, both proliferation and its rate is critical to experiments in a wide variety of<br />
disciplines for example, immunology, cardiovascular disease and cancer. For this reason, over the years a number<br />
of assays have been developed to measure these processes and the gold standard assay has remained the<br />
uptake of 3H Thymidine. Examples of other assays developed to obviate the use of 3H label are MTT Formazan<br />
(colorimetric), BrdU ELISA (fluorescence) and Acid Phosphatase (colorimetric). However, all these assays involve<br />
a number of steps. Using the Acumen Explorer laser scanning fluorescence microplate cytometer a simple<br />
homogenous quantitative assay can be performed in both 96- and 384-well plate formats. Experiments described<br />
here show how the Acumen Explorer can be used to follow the basal proliferation of both adherent and suspension<br />
cell lines and the effect of inhibitors Pacilitaxel and Anisomycin quantified.<br />
WP054<br />
Eric Schmidt<br />
Ahura Corporation<br />
46 Jonspin Road<br />
Wilmington, Massachusetts 01887<br />
eschmidt@ahuracorp.com<br />
Innovations in Photonics for Biotech Applications<br />
Co-Author(s)<br />
Daryoosh Vakhshoori<br />
Biotech researchers have had to adapt their techniques around the tools which have been available, often with<br />
high expense and sub-optimal results. Now, thanks to billions of dollars invested in telecommunications, new<br />
classes of instruments based on novel technologies are being directed at the problems confronting the biotech<br />
community. Semiconductor lightsources and MEMs offer high reliability, long life, and small packages with very<br />
high efficiency, making them an ideal replacement for traditional lasers and optics. With all these advantages,<br />
scientists and engineers are thinking “outside the box” combining cross disciplinary know-how to create new<br />
classes of instruments with advanced capabilities including: multiple wavelengths, modulation, scanning, and most<br />
of all portability. Breakthrough innovations come when incremental innovations converge on a new market. In the<br />
case of photonics, innovations underwritten by billions of dollars of telecom investment are bearing fruit in the<br />
medical, scientific and industrial field. Teams of physicists and engineers are working to develop low-cost mobile<br />
optical platforms which should revolutionize drug discovery by putting “Main Frame” power onto the researchers’<br />
desk top.These next generation systems will incorporate multiple semiconductor lightsources, the optics to<br />
manipulate the photons, the apparatus to expose the samples and the detectors to measure the results in “Lap<br />
Top” size instruments. The vision is to utilize the breakthroughs in semiconductor lasers and MEMs to provide<br />
maintenance free, reliable, high efficiency packages which offer more functionality than what is presently available.<br />
POSTER ABSTRACTS
WP055<br />
Donald Schwartz<br />
DRD<br />
83 Pine Street<br />
West Peabody, Massachusetts 01960<br />
donschwartz@drddiluter.com<br />
218<br />
Co-Author(s)<br />
Donald S. Martin<br />
First Dual Resolution Syringe (DRS), Using Differential Displacement, for Practical Contact-<br />
Free Nanoliter Transfer of Discrete Samples or Within-tip Mixtures, and Extreme Dilutions<br />
The unchallenged aspiration supremacy and reliability of smooth motor-controlled positive displacement syringe<br />
devices has been sullied by small seal and bubble hassles and recently overwhelming demands for ever-finer<br />
resolution without loss of blowout power. Awesome complexity has spawned to try to compensate for the<br />
shortcomings. The Dual Resolution Syringe (DRS) (patented and patent-pending) is a syringe with a glass barrel<br />
the same ID as a 1 mL syringe, with a sturdy little spring-loaded piston that is slightly smaller than the glass barrel<br />
ID. The piston can move in and out of the barrel, coordinating with the plunger. When the two move together<br />
(DRD Differential Mode) the cross sectional area difference and resolution are like a 10 µL (or even 1 µL) syringe,<br />
aspirating smoothly down to 10 nL. Then the plunger moves alone (DRD Bulk Mode), delivering abundant flow<br />
power (like a 1mL syringe) to blow the minute sample out, which it can do at well over 1.5 meters/sec through any<br />
diameter tip, intact and without damage (Blastoff), to its microplate, array, spotting or Maldi target. Samples and<br />
reagents can also be mixed inside certain tips and the mixture delivered contact-free.<br />
DRD has thus endowed the syringe with its Differential Displacement capability. This preserves the classic<br />
strengths of venerable classic syringe technology, eliminates shortcomings, and enormously enhances its range<br />
and analytical flexibility. The elimination of small seals and crannies, and the high flow priming throughout, also<br />
greatly increase reliability and longevity. The DRS directly replaces a conventional syringe in its host system.<br />
Substituting a DRS for a conventional 250 µL syringe, for example, immediately gives 25X finer resolution for<br />
aspiration, 4X more flow power for delivery, and within-tip mixing capability. This model can also aspirate a 10 nL<br />
sample and dilute it 30,000:1. The DRS debuts as individual units and as a bank of eight 9 mm-spaced units for<br />
contact-free transfer of drops down to 10 – 25 nanoliters.
WP056<br />
Steven Sheridan<br />
Millipore<br />
Life Sciences<br />
17 Cherry Hill Drive<br />
Danvers, Massachusetts 01923<br />
steven_sheridan@millipore<br />
219<br />
Co-Author(s)<br />
Sonia Gil<br />
Libbey Kellard<br />
Scaling From 96- to 384-Well Assay Platforms: Characterization of Receptor-Ligand Binding<br />
Using 384-Well Filter Plates Optimized For Maximum Radiation Detection<br />
A significant portion of the drug discovery process involves the systematic screening of large compound libraries<br />
for specific binding to various cellular receptors. For decades, heterogeneous filter binding assays have been<br />
used for this purpose, particularly under more challenging conditions such as when the receptor is prepared<br />
from unpurified tissue homogenates or cell membrane fragments. To date, most screening has been performed<br />
on 96-well platforms. However, with a rapidly increasing number of compounds available from natural product<br />
isolation and combinatorial chemistry, a bottleneck has been created which has made it necessary to develop<br />
higher throughput platforms that make it possible to perform adequate library screening in a timely and cost<br />
effective manner. A 384-well filter plate has been developed and optimized for automated radiometric receptorligand<br />
binding assays. This 384-well filter binding format increases throughput of receptor-ligand binding screening<br />
without sacrificing sensitivity, robustness or precision. The design is optimized for maximum radiation detection<br />
and is compatible with coincidence scintillation counting making it possible to achieve the same low-end sensitivity<br />
as attained with a 96-well plate. Using parallel experimental procedures, reagents and receptor systems, data are<br />
presented that demonstrate the scalability of radiometric receptor-ligand binding assays from a 96- to a 384-well<br />
format. The higher density of the 384-well filter plate allows for quantitative receptor-ligand binding assays in a<br />
robust, fast, and reagent saving format.<br />
WP057<br />
Christopher Silva<br />
Molecular Devices Corporation<br />
1311 Orleans Drive<br />
Sunnyvale, California 94089<br />
chris_silva@moldev.com<br />
SpectraMax M2 Multi-detection System Allows Validated Microplate-based Fluorescence and<br />
Absorbance Assays in a GLP/GMP Environment<br />
The SpectraMax M2 multi-detection microplate reader with dual-mode cuvette port allows a wide rage of<br />
fluorescence (FI) and absorbance (Abs) assays to be converted into QC, manufacturing and pre-clinical<br />
environments. Dual monochromators, uv/vis wavelength range, PathCheck, fluorescence, and absorbance<br />
validation plates with integrated SoftMax Pro data analysis and FDA 21 CFR Part 11 compliant tools and an<br />
aut<strong>omation</strong> interface make the transfer of assays from research and development to the GLP/GMP environment<br />
straightforward.<br />
POSTER ABSTRACTS
WP058<br />
Michael Simonian<br />
Beckman Coulter, Inc.<br />
Biomedical Research Division<br />
P.O. Box 3100<br />
Fullerton, California 92834-3100<br />
mhsimonian@beckman.com<br />
Using the Biomek ® 3000 Laboratory Aut<strong>omation</strong> Workstation to Interface 2D Liquid<br />
Chromatography With Mass Spectrometry for Multidimensional Proteome Profiling<br />
220<br />
Co-Author(s)<br />
Matthew Cu<br />
Edna Betgovargez<br />
Graham Threadgill<br />
The discovery stage of proteome profiling typically involves the comparison of different states of a cell or tissue.<br />
One approach utilizes fractionation of the proteome followed by mass spectrometry (MS). A two-dimensional,<br />
liquid chromatographic fractionation system, the ProteomeLab PF 2D, followed by a third dimension with MALDI-<br />
TOF MS has been used for this approach. The first dimension separation is chromatofocusing where proteins are<br />
separated by pI and collected in fractions based on pH intervals. Upwards of 20 pI fractions are then separated in<br />
a second dimension by reversed-phase chromatography. From each second dimension run, 80-90 fractions can<br />
be collected. To accommodate the large number of fractions generated by the first two dimensions for subsequent<br />
MS analysis, the Biomek 3000 Laboratory Aut<strong>omation</strong> Workstation was used. The Biomek 3000 Laboratory<br />
Aut<strong>omation</strong> Workstation prepared and spotted the fractions from the second dimension runs along with the<br />
appropriate matrix on a 384-well format MALDI target. The third dimension measures the mass to charge ratio of<br />
the proteins. Human plasma and serum were compared with this multidimensional approach. Although albumin<br />
constitutes 55% of blood proteins, lower abundant proteins and proteins of 4-12 kD in the same pI fraction as<br />
albumin were detected by MALDI-TOF MS. The Biomek 3000 Laboratory Aut<strong>omation</strong> Workstation facilitated the<br />
complete analysis of a fractionated proteome by removing the potential bottleneck resulting from the large number<br />
of samples collected from the first two dimensions.<br />
WP059<br />
Katja Sippola<br />
PerkinElmer Life and Analytical Sciences, Wallac Oy<br />
Biochemistry<br />
P.O. Box 10<br />
TurkuFIN-20101 Finland<br />
katja.sippola@perkinelmer.com<br />
Co-Author(s)<br />
Joni Helenius, Sanna Rönnmark,<br />
Maija-Liisa Mäkinen, Jari Suontausta,<br />
Christel Gripenberg-Lerche, Satu Kovanen<br />
Automated DELFIA ® Filtration Assays in 96- and 384-well Format for GPCR Studies<br />
Time-resolved fluorescence enhancement technique DELFIA ® enables development of highly sensitive assays<br />
for high throughput screening. We have developed a family of Europium-labeled peptides and proteins designed<br />
for ligand receptor binding assays, as well as a Europium-labeled GTP binding kit for functional assays. These<br />
products provide an excellent non-radioactive alternative that is both stabile and sensitive. Ligand binding assays<br />
based on filtration are the most widely used assays to characterize drug candidates for specific receptors. The<br />
procedure requires liquid handling, incubation and filtration steps that are typically performed manually. We have<br />
automated the Europium-label utilizing binding assays both in 96- and 384-well format using an assay workstation<br />
that allows fully hands-free operation. The results demonstrate comparable or superior performance to manual<br />
assays with greatly increased through-put.
WP060<br />
Stephen Skwish<br />
Merck & Company, Inc.<br />
Aut<strong>omation</strong> & Informatics<br />
P.O. Box 2000<br />
Rahway, New Jersey 07065<br />
stephen_skwish@merck.com<br />
FIZICS: A Novel Macro Imaging System<br />
221<br />
Co-Author(s)<br />
Francisco Asensio, Greg King, Glenn Clarke,<br />
Gary Kath, Michael J. Salvatore, Claude Dufresne<br />
Constantly improving biological assay development continues to drive technological requirements. Recently, a<br />
specification was defined for capturing white light and fluorescent images of agar plates ranging in size from the<br />
NUNC Omni tray (96-well footprint, 128 X 85 mm) to the NUNC BioAssay Dish (245 X 245 mm). An evaluation of<br />
commercially available products failed to identify any system capable of fluorescent macro imaging with discrete<br />
wavelength selection. To address the lack of a commercially available system, a custom imaging system was<br />
designed and constructed. This system provides the same capabilities of many commercially available systems<br />
with the added ability to fluorescently image up to a 245 mm 2 area using wavelengths in the visible light spectrum.<br />
WP061<br />
Ginger Smith<br />
GlaxoSmithKline<br />
High Throughput Biology<br />
5 Moore Drive<br />
Durham, North Carolina 27709<br />
ginger.r.smith@gsk.com<br />
Aut<strong>omation</strong> Systems Run in Parallel<br />
Co-Author(s)<br />
Amy Siu, Renae Crosby, Jim Liacos,<br />
Cathy Finlay, Jimmy Bruner<br />
The High Throughput Biology department at GlaxoSmithKline develops in vitro models to better predict the<br />
efficacy of compounds in the clinic. The aut<strong>omation</strong> team is responsible for developing methodologies that are<br />
robust, flexible and scaleable based on the specific science of the assay and required throughput. One of the<br />
strategies of the aut<strong>omation</strong> team is to provide identical platforms on which any one assay can be run in parallel<br />
or simultaneously and yield the same results. This poster describes the validation of an IL-1 Stimulated p38 Map<br />
Kinase translocation assay in HFF cells run on three separate Map C II’s on the same day and compares the<br />
results to the assay run by hand.<br />
POSTER ABSTRACTS
WP062<br />
Norbert Stoll<br />
University of Rostock<br />
Institute of Aut<strong>omation</strong><br />
R.-Wagner-Strasse 31<br />
Rostock18119 Germany<br />
norbert.stoll@uni-rostock.de<br />
Automated Sampling System for Parallel Autoclaves in High Throughput Chemistry<br />
222<br />
Co-Author(s)<br />
Wof-Dieter Heinitz<br />
Hans-Joachim Stiller<br />
KerstinThurow<br />
The paper describes a parallel sampling system for autoclaves for application in combinatorial chemistry. For<br />
kinetic investigations a sampling for analytical reasons has to be performed. The system is able to take samples<br />
from 5 / 10 autoclaves under pressures up to 60 bars. Up to 10 samples for every reactin system will be filled into<br />
a gc-vial placed on a base plate in microplate format. The system is fully automated and can be included into<br />
integrated systems.<br />
WP063<br />
Regina Stoll<br />
University of Rostock<br />
Institute of Occupational Medicine<br />
St. Georg Strasse 108<br />
Rostock18055 Germany<br />
regina.stoll@med.uni-rostock.de<br />
Real-time Physical Fitness Validation by Automated Respiratory Analysis<br />
Co-Author(s)<br />
Norbert Stoll<br />
Physical fitness is one of the most important status information about a patient in internal medicine as well as<br />
in occupational and sports medicine. Unfortunately, the most significant parameter VO2max (maximal oxygen<br />
uptake) has to be determined under vita-maxima conditions. To decrease the risk for the patient and to save time<br />
a determinmation of VO2max at submaximum level has to be performed. The paper describes an automated<br />
real-time method on a basis of analytical liearizable functions with regression equations for VO2-P- and VCO2-Pfunctions<br />
for tests under increasing load P conditions. The system has been included into and tested with the data<br />
of an ergospirometry measurement system. The results are shown in relation to other post-run off-line heart rate<br />
based estimation methods and show the advantage of the approach.
WP064<br />
Rowan Stringer<br />
Novartis Horsham Research Centre<br />
Biology 1<br />
Wimblehurst Road, Horsham, West Sussex<br />
Nr LondonRH12 5AB United Kingdom<br />
rowan.stringer@pharma.novartis.com<br />
96-Well Caco-2 transport Model for Drug Permeability Studies<br />
223<br />
Co-Author(s)<br />
Elizabeth Willmott<br />
Rachael Profit<br />
Sarah Beech<br />
Paul Nicklin<br />
A 96-well Caco-2 permeability screen has been established for drug absorption profiling. After 21 days of culture<br />
on Millipore MultiScreen ® inserts, light microscopy showed the formation of intact monolayers of polarised<br />
columnar cells. Ultra-structural studies confirmed the presence of a microvilli brush-border and tight junctions<br />
between cells. Monolayers had low permeability towards the paracellular marker, [ 14 C]mannitol (Papp = 0.21±0.06<br />
cm/s), and high permeability towards propranolol (Papp=19.6±4.6 cm/s). This system had good intra- and interplate<br />
reproducibility for reference compounds (e.g., CV = 18% for the Papp of 15 drugs, over 16 different test<br />
occasions). Moreover, a good correlation was observed between their in vitro Papp and the fraction absorbed<br />
in man. The Caco-2 monolayers were also Pgp-competent, having a net efflux transport for known substrates –<br />
e.g., efflux ratios for acebutolol (~100-fold), quinidine (~20-fold) and verapamil (~3-fold). The 96-well screen<br />
was integrated with an automated bioanalytical platform, enabling high compound throughput. For example,<br />
a set of 100 marketed drugs and 1200 discovery project compounds were screened in 16 weeks. This model<br />
system differentiated compound classes having low and high permeability as well as highlighting those having a<br />
susceptibility for efflux transporters.<br />
WP065<br />
Michael Su<br />
Molecular Devices Corporation<br />
Instrument R&D<br />
1311 Orleans Drive<br />
Sunnyvale, California 94089<br />
Michael_su@moldev.com<br />
Co-Author(s)<br />
Jinfang Liao<br />
Evelyn McGown<br />
Dual-Glo Luciferase Assay Measurements in the LMax II 384 Microplate Luminometer<br />
and the Analyst ® GT Multimode Reader<br />
Reporter gene assays are used to study eukaryotic gene expression. Dual genetic reporters are commonly used in<br />
transient transfections of cultured cells to minimize experimental variability caused by differences in cell number,<br />
viability or transfection efficiency. One plasmid containing the experimental reporter gene (coupled to a regulated<br />
promoter) is cotransfected with a second plasmid containing a control reporter gene (coupled to a constitutive<br />
promoter). Bioluminescent reporter systems using firefly and Renilla luciferases are widely used as co-reporters<br />
because both assays are easy and sensitive. Previously, luciferase reporter assays have been “flash” assays that<br />
must be read within seconds of reagent addition and require integrated injectors in the luminometer. Recently,<br />
Promega introduced a Dual-Glo Luciferase assay System for high throughput analyses. The signals are stable for<br />
2 hours after reagent addition and integrated injectors are not necessary.In this study, we optimized measurement<br />
parameters and compared Dual-Glo Luciferase assay results on the LMax II 384 Microplate Luminometer and<br />
the Analyst ® GT Multimode Reader. Due to the stability of the luminescent signal, both instruments are well suited<br />
for integrating this dual luciferase assay into high throughput screening lines.<br />
POSTER ABSTRACTS
WP066<br />
Yu Suen<br />
Beckman Coulter, Inc.<br />
Biological Systems Operation<br />
4300 N. Harbor Boulevard<br />
Fullerton, California 92834<br />
Ysuen@beckman.com<br />
224<br />
Co-Author(s)<br />
Keith Roby<br />
Javorka Gunic<br />
Michael H. Simonian<br />
Aut<strong>omation</strong> of Caco-2 Cell Preparation, Drug Permeation and Transport Assay on the Biomek<br />
3000 Laboratory Aut<strong>omation</strong> Workstation<br />
Incorporating predictive ADME (absorption, distribution, metabolism and elimination) assays in earlier stages<br />
of drug discovery can help in rejecting candidate molecules that lack necessary pharmacological properties. A<br />
human colorectal adenocarcinoma cell line, Caco-2 can be used to assess absorption, permeation and efflux<br />
transport properties of a candidate drug. A high throughput Caco-2 assay can provide useful information for lead<br />
optimization in the drug discovery industry. This poster describes the use of Beckman Coulter’s Biomek 3000<br />
Laboratory Aut<strong>omation</strong> Workstation to automate Caco-2 cell preparation and differentiation in the BD Falcon<br />
HTS 96-Multiwell Insert System. Additionally, Beckman Coulter’s Biomek 3000 Laboratory Aut<strong>omation</strong> Workstation<br />
was used to automate the compound permeability, efflux testing and sample collection. We have demonstrated<br />
intact and functional Caco-2 monolayers after 21 days of culture manipulation by the Biomek 3000 Laboratory<br />
Aut<strong>omation</strong> Workstation. The Caco-2 monolayer provided a selective barrier for transcellular and carrier-mediated<br />
efflux transport of different drugs. The permeability ranking of drug standards using the Caco-2 assay system<br />
matched well with the Potential Internal Standards suggested by the FDA. The bi-directional transport studies<br />
verified functional P-glycoprotein efflux pump activities. The Biomek 3000 Laboratory Aut<strong>omation</strong> Workstation<br />
facilitated Caco-2 assay implementation, reduced the chance of contamination and minimized the intensive<br />
requirement of sterile skills. The automated Caco-2 assay can be used for high throughput screening of drug<br />
candidates for absorption<br />
WP067<br />
Joseph Suzow<br />
Pediatrix Screening<br />
Molecular Genetics<br />
90 Emerson Lane<br />
Bridgeville, Pennsylvania 15017<br />
jsuzow@yahoo.com<br />
Application of Aut<strong>omation</strong> for Clinical Newborn Screening: Detection of Hearing Loss<br />
Associated Cytomegalovirus<br />
Co-Author(s)<br />
Zhili Lin<br />
Michelle Lage<br />
Edwin W. Naylor<br />
This study consists of a clinical newborn screening application for the detection of cytomegalovirus (CMV). CMV<br />
infection in the newborn is associated with the onset of hearing loss. Early detection of hearing loss is critical<br />
for normal child development. Specimens are collected on a paper filter card. After drying, a small paper disk is<br />
punched into each well of a 96-well plate. A Beckman Coulter Core System is then used to extract DNA from<br />
the blood spot and set up a PCR reaction. The Core System consists of a Biomek FX liquid handler, an ORCA<br />
arm, peripheral heat blocks, stacker carousels, plate sealer, and plate piercer. A 20µl PCR reaction is set up in<br />
a 384 well format. The reaction consists of FRET hybridization probes combined with common PCR reagents.<br />
Following amplification, product is detected using the Roche Light Typer instrument. Detection involves monitoring<br />
of fluorescence during a post PCR melting cycle. A mathematical derivative of the resulting melting slope is used<br />
to convert the slope to a peak. The apex of the peak corresponds to the melting temperature of the detection<br />
probe. Detection of a peak indicates the presence of amplified CMV DNA. The Light Typer instrument is capable of<br />
detecting 384 specimens in a single 10 minute run. We have demonstrated the ability to screen greater than 2000<br />
clinical specimens each day.
WP068<br />
Anny Tangkilisan<br />
Symyx Technologies<br />
Life Sciences<br />
3100 Central Expressway<br />
Santa Clara, California 95051<br />
atangkilisan@symyx.com<br />
Application of High Throughput Screening for Pre-formulations Using the Symyx<br />
Technologies Workflow<br />
A high throughput crystallization system has been developed at Symyx Technologies with applications in preformulations.<br />
Capability of running over 384 different crystallizations/day with four different independently<br />
controlled heating/cooling environments and rapid serial screening will be described. Crystallizations are preformed<br />
on the Symyx Technologies developed crystallization unit and screened using birefringence, Raman, XRD and a<br />
Symyx parallel melting point apparatus. All the screening data is stored into a database and sorted by using a<br />
proprietary software package developed at Symyx Technologies. Examples of a salt selection of ephedrine and a<br />
polymorph study of phenylbutazone using this system will also be presented.<br />
WP069<br />
Kerstin Thurow<br />
University of Rostock<br />
Institute for Aut<strong>omation</strong><br />
R.-Wagner-Strasse 31<br />
Rostock18119 Germany<br />
Kerstin.Thurow@uni-rostock.de<br />
Automated Screening of Cytotoxic Compounds<br />
225<br />
Co-Author(s)<br />
Kristin Entzian<br />
Toxicology and pharmacological activity of cytotoxic drugs where tested in a robotic high throughput screening<br />
cell assay (HTS), which allows prediction for the efficacy and for toxic effects of the drug. A robotic HTS system<br />
was assembled and programmed to seed cells in 96 well plates. The cell lines are incubated with a serial dilution<br />
of the test substance. After incubation for 2 to 3 days are metabolic indicator dye added. The remaining metabolic<br />
rate is measured after an additional incubation for several hours in a plate reader. The accumulation of dye in the<br />
remaining vital cells is plotted against the dilution of the test substance. The concentration of test substance where<br />
the absorbance is half of the maximum is considered the pharmacological activity or the toxic limit respectively.<br />
The activity is calibrated against the activity of substances with known cytotoxic activity and toxicity. The activity<br />
of Busulfan, Piposulfan and of several commonly used formulation components such as Cremophor EL, PEG<br />
400, DMSO, Acetone, Ethanol and Cyclodextrin were tested against several carcinoma and non-carcinoma cell<br />
lines.The assay is reproducible and is able to rank cytotoxic compounds according to relative cytotoxicity. This<br />
assay is suitable for screening of cytotoxic compounds for cytotoxic activity as well as for general toxicity. The<br />
assay is also indicative of cytotoxic effects of excipients.<br />
POSTER ABSTRACTS
WP070<br />
Melissa Trout<br />
Code Refinery<br />
2201 Candun Drive, Suite 101<br />
Apex, North Carolina 27523<br />
mtrout@code-refinery.com<br />
Integrating Software Validation Throughout Software Development<br />
226<br />
Co-Author(s)<br />
Samir Dandekar<br />
Mike Brown<br />
Code Refinery’s software validation methodology allows a customized approach for all phases of the software<br />
development life cycle. Closely integrating validation into the development life cycle improves software quality<br />
and reduces cost and effort when attempting to obtain product approval from regulatory agencies. Code<br />
Refinery’s focus relies heavily on three standard concepts: communication, documentation and testing. Efficient<br />
communication among team me<strong>mbers</strong> is vital during the review of validated documents, goals and project plans.<br />
To accurately include validation in a software product’s development, documentation must verify that each step in<br />
the development life cycle fulfills the requirements of the previous stage in order to meet the needs of the ultimate<br />
end user. Lastly, comprehensive testing verifies that the software consistently meets user needs, fulfills safety<br />
regulations and operates without defects in critical functionality. Code Refinery’s integrated validation will control<br />
and minimize the variables impacting software development. The software validation methods and documents<br />
outlined in this poster illustrate the effectiveness of structured, supportive software validation.<br />
WP071<br />
Robert Umek<br />
Meso Scale Discovery<br />
9238 Gaither Road<br />
Gaithersburg, Maryland 20877<br />
rumek@meso-scale.com<br />
High Throughput Multiplexed Assays for Biomarkers and Phosphoproteins<br />
Co-Author(s)<br />
Paula Denney Eason<br />
Jenny Ly<br />
Jacob N. Wohlstadter<br />
The intense interest in biomarkers and phosphoproteins arises from their importance in critical processes. These<br />
proteins are best measured in a multiplex format: understanding a given signal transduction pathway often requires<br />
measuring multiple phosphoproteins and samples containing biomarkers (e.g., serum) are often precious. In this<br />
poster we present multiplex immunoassays for biomarkers and phosphoproteins using Meso Scale Discovery’s<br />
(MSD’s) Multi-Array platform. These assays are conducted in MSD’s Multi-Spot plates, using plates pre-coated<br />
with capture antibodies for each biomarker or phosphoprotein, a cocktail of labeled detection antibodies and other<br />
related assay reagents. The assays offer excellent sensitivity, compatibility with complex samples such as serum or<br />
blood, and a wide dynamic range that avoids the necessity of multiple dilutions.
WP072<br />
Surekha Vajjhala<br />
Nanostream, Inc.<br />
580 Sierra Madre Villa Avenue<br />
Pasadena, California 91107<br />
surekha.vajjhala@nanostream.com<br />
Rapid Compound Purity Screening using the Nanostream Veloce System<br />
227<br />
Co-Author(s)<br />
Li Zhang<br />
Paren Patel<br />
Sergey Osechinskiy<br />
Over the past decade, advances in combinatorial chemistry and target characterization have significantly increased<br />
the number of drug candidates and targets available to drug discovery screeners. To ensure a meaningful screen,<br />
an increasing number of pharmaceutical companies are now evaluating compound purity. The Nanostream Veloce<br />
system, together with 24-column Brio cartridges, offers a novel approach to micro parallel liquid chromatography.<br />
This system allows users to achieve unprecedented throughput for standard assays while matching the<br />
performance of conventional LC instrumentation, thus enabling a cost effective way to routinely monitor compound<br />
purity with minimal modification to current methods and work flow. This poster presents results of a study of<br />
pharmaceutical compound library samples using the Veloce system. Individual chromatograms, percent purity<br />
results, study duration and total solvent consumption are compared to results obtained using conventional HPLC.<br />
WP073<br />
Scott Van Arsdell<br />
Pierce Biotechnology, Inc.<br />
Research and Development<br />
30 Commerce Way<br />
Woburn, Massachusetts 01801<br />
svanarsdell@perbio.com<br />
SearchLight Proteome Arrays: Multiplexed Assays for High Content Screening<br />
Co-Author(s)<br />
Rajiv Pande<br />
Christine Burns<br />
A SearchLight Proteome Array for quantitative detection of proteins is described. The array is created by<br />
spotting 25 capture antibodies in a 5 X 5 pattern at the bottom of each well in a 96-well polystyrene microtiter<br />
plate. Target proteins are ‘captured’ by appropriate arrayed antibodies upon sample introduction. Biotinylated<br />
secondary antibodies are added and specifically bind the captured protein. Streptavidin-horseradish peroxidase<br />
conjugate is subsequently added to tag the ‘biotinylated antibody – protein – capture antibody’ sandwich, followed<br />
by the addition of a chemiluminescent substrate. The plate is imaged with the SearchLight CCD Imaging and<br />
Analysis System, and the density of each spot is quantified. A cocktail of recombinant target proteins is assayed<br />
on the plate to generate standard curves and allow quantification of analytes in the samples. We describe the use<br />
of a SearchLight 25–plex array to quantitate a panel of cytokines and chemokines (human IL-1 alpha, IL-2, IL-4,<br />
IL-5, IL-6, IL-8, IL-10, IL-12, IL-13, IL-18, IFN-gamma, TNF-alpha, RANTES, Eotaxin, MDC, TARC, I-309, MIP-1<br />
alpha, MIP-1 beta, MCP-1, GRO-alpha, NAP-2, IP-10, MIP-3 alpha, and MIP-3 beta). The array was utilized to<br />
monitor cytokine and chemokine expression in mitogen stimulated peripheral blood mononuclear cells (PBMCs).<br />
We demonstrate the applicability of SearchLight Proteome Arrays for high content screening by simultaneously<br />
quantifying 25 analytes per well (2400 data points in a 96-well plate, up to 80 samples per plate). SearchLight<br />
assays are simple, very sensitive and rapid (~2hrs); and the technology is compatible with plate based robotics.<br />
POSTER ABSTRACTS
WP074<br />
Sofia Vikstrom<br />
PerkinElmer Life and Analytical Sciences<br />
R&D<br />
Wallacoy P.O. Box 10<br />
TurkuFIN-20101 Finland<br />
sofia.vikstrom@perkinelmer.com<br />
A Cell-Based Multi-label DELFIA Assay for Measuring Phosphorylation of Proteins<br />
228<br />
Co-Author(s)<br />
Christel Gripenberg-Lerche<br />
Pertti Hurskainen<br />
Ilkka Hemmilä<br />
We have developed a cell-based DELFIA assay to test compounds for their ability to affect the signal transduction<br />
in the cell, or to modulate cellular stress. The Cell DELFIA assay is performed in 96-well microplate format and<br />
replaces classical gel-electrophoresis and Western blotting techniques by the utlilization of cells as the target of<br />
the assay instead of isolated proteins. The assay has been used to detect changes in the phosphorylation level<br />
of different proteins using lanthanide-labeled antibodies. This cell-based DELFIA assay provides the means for<br />
identifying multiple parameters in the cell. This approach is used for detecting two or more kinases or events, in<br />
the cell, when using one of the lanthanide labels as a control for the number of cells per well, by the binding to a<br />
cellular component which remains at a constant level.<br />
WP075<br />
Erich von Roedern<br />
Aventis Pharma<br />
Chemistry<br />
Building G838<br />
Frankfurt65926 Germany<br />
erich.roedernvon@aventis.com<br />
SynCar: A New Automated Solution Phase Synthesis Platform for Medicinal Chemistry<br />
Co-Author(s)<br />
Angelika Weber<br />
Hans-Ulrich Stilz<br />
Aut<strong>omation</strong> of all the important functionalities of solution phase synthesis into one integrated system is still<br />
a challenge. The demands on such a system were to provide with short cycle times, series of 50 – 250 pure<br />
compounds in a 20 – 50 mg scale for target class libraries and lead optimization. In a co-development between<br />
Aventis and Accelab GmbH, Kusterdingen, Germany we designed an unprecedented system of independent<br />
workstations connected by a shuttle transfer system produced by Montech, Derendingen, Switzerland. At the<br />
moment seven modular workstations process four reaction samples on each shuttle in parallel such as synthesis<br />
(temperature control and liquid reagent handling), filtration, liquid-liquid extraction, evaporation, weighing, solid<br />
phase extraction and HPLC-MS analysis. The modular design allows an easy expansion for future needs. The<br />
system can be continuously loaded at any time with shuttles and every shuttle can have its own workflow. Design<br />
and engineering combines a high flexibility with high throughput.
WP076<br />
Wei Wang<br />
Pfizer<br />
PGRD<br />
2800 Plymouth<br />
Ann Arbor, Michigan 48105<br />
wei.wang@pfizer.com<br />
Aut<strong>omation</strong> Tools To Aide Information Management in a Pharmaceutical Discovery<br />
Environment<br />
229<br />
Co-Author(s)<br />
Brian Boyd<br />
Neelesh Nundkumar<br />
Mark Proefke<br />
A chemist often needs an instant access to analytical NMR data obtained in Open Access (OA) NMR lab in<br />
modern pharmaceutical discovery. Although OA NMR is not a new concept, we’ve found that there are still plenty<br />
opportunities for improvement, especially in terms of interconnecting different software to create an integrated lab<br />
environment. Here we present a suite of laboratory aut<strong>omation</strong> tool kits that has helped the management of datainformation-knowledge<br />
flow in our lab. Specific examples include a easy-to-use VNMR interface called MyNMR,<br />
and a web-based SQL application dubbed Data Pfinder.<br />
POSTER ABSTRACTS
WP078<br />
Mike Wheeler<br />
Guy’s and St. Thomas’ Hospital Trust<br />
Chemical Pathology<br />
Lambeth Palace Road<br />
London SE1 7EH United Kingdom<br />
mike.wheeler@kcl.ac.uk<br />
Introduction of Aut<strong>omation</strong> Into Clinical Laboratories in the UK<br />
230<br />
Co-Author(s)<br />
Carolyn Piggott<br />
Guy’s and St. Thomas’ Hospital Trust<br />
Stephen Halloran<br />
University of Surrey<br />
There has been a slow and cautious approach to aut<strong>omation</strong> in the UK and few laboratories have either total<br />
aut<strong>omation</strong> or pre-analytical systems.Laboratories have few sources to help them to decide whether aut<strong>omation</strong><br />
is appropriate for them, which system to choose and on reliability. We have carried out an off-site evaluation of<br />
seven different pre-analytical systems (Bayer, Beckman, Dade Behring, Olympus, Ortho-Clinical Diagnostics,<br />
Roche, and Tecan (Abbott), in 10 hospitals. Information was gathered on site and system information before<br />
and after introduction of the pre-analytical system; procurement (purchasing) procedures; installation and<br />
implementation procedures; fulfilment of expectations; system reliability and customer support, and detailed<br />
systems specifications. Choice of total aut<strong>omation</strong> or stand-alone pre-analytical instrument was not related to size<br />
of hospital/workload. Several project managers emphasised the need to identify the processes that would benefit<br />
from aut<strong>omation</strong>. Although sites commented on the reduction in health and safety risks, there were some concerns<br />
about safety e.g., with the Roche and Olympus systems. Expectations were not realised in all cases. A reduction<br />
of turnaround time was not always achieved especially if the system could not handle the maximum workflow.<br />
Common problems across the sites were system errors due to poorly attached barcode labels and patient address<br />
labels leading to errors with centrifuges, sample holders and level sensing units. Poor definition barcode labels and<br />
sample tube caps were also a source of problems. Other problems were linked to slow response of/insufficient<br />
support engineers and lack of spare parts in the UK leading to extended downtimes.<br />
WP079<br />
Ian Whitehall<br />
TTP LabTech Ltd<br />
Melbourn Science Park, Cambridge Road<br />
RoystonSG8 6EE United Kingdom<br />
inw@ttplabtech.com<br />
Primary HTS Neurite Outgrowth Assay Using the Acumen Explorer<br />
Co-Author(s)<br />
John Budd<br />
Paul Wylie<br />
Wayne Bowen<br />
The discovery and characterization of compounds and new chemical entities that promote or suppress neurite<br />
outgrowth is of great importance in the continued search for therapies to treat central nervous system diseases<br />
such as Alzheimer’s and Parkinson’s disease. A simple, rapid, quantitative neurite outgrowth assay has now been<br />
developed for the Acumen Explorer laser scanning fluorescence detection system, in both 96- and 384-well<br />
plate format. Experiments described have used the SH-SY5Y human neuroblastoma cell line. Conditions were<br />
established where differentiation and subsequent neurite formation were stimulated over a 3 to 7 day period by<br />
chronic exposure to all-trans retinoic acid (ATRA). Neurite formation was then quantified by addition of the dye<br />
4-(4-(dimethylamino)styryl)-N- methyl pyridinium iodide (4-Di-1-ASP) (Molecular Probes, Inc.). Plates were then<br />
scanned on the Acumen Explorer. Using the unique capability of the Explorer the inclusion of differentiated and<br />
non-differentiated cells in appropriate populations was performed to provide neurite number per well.
WP080<br />
Chris Willis<br />
Ambion, Inc.<br />
Research and Developement<br />
2130 Woodward Avenue, Suite 200<br />
Austin, Texas 78741<br />
cwillis@ambion.com<br />
High Throughput Viral RNA Isolation from Swab, Serum and Plasma<br />
231<br />
Co-Author(s)<br />
Xingwang Fang<br />
Michael A. Siano<br />
The molecular diagnosis is getting widely adapted because of its high sensitivity and high throughput with short<br />
turn around time, which is often based on RNA isolation and quantitative RT-PCR. The widely used Trizol method<br />
for RNA isolation (phenol extract followed by ethanol precipitation) is very difficult to scale up. We have developed<br />
a magnetic beads based RNA isolation, which is high throughput and can be easily automated. This technology<br />
enables viral RNA isolation from swabs with a very simple procedure in 96-well plate to efficiently recover RNA<br />
without sample cross contamination. It has been successfully used for high throughput diagnosis of Exotic<br />
Newcastle Disease (END) shortly after END breakout in United States last October. This technology is also very<br />
useful for viral RNA isolation from serum and plasma samples, as well as total RNA isolation from culture cells<br />
and small pieces of tissue. Results of RNA isolation from various liquid samples using this technology will be<br />
presented. Applications and the advantage of this technology will be discussed.<br />
WP081<br />
Steven Wilson<br />
Joint Genome Institute<br />
Instrumentation<br />
2800 Mitchell Drive<br />
Walnut Creek, California 94608<br />
sewilson@lbl.gov<br />
Integrating Microsoft’s .NET Platform Into the DOE’s Production Genomics Facility<br />
The DOE Joint Genome Institute’s semi-automated DNA sequencing production line requires operators to manually<br />
transfer batches of sample plates between instruments. Manual handling of plates presents opportunities for plate<br />
errors through plate mixups, mismatches, and lost plates. Microsoft’s .NET platform is being used at JGI to assist<br />
operators in accurate plate tracking during manual handling. .NET’s ease of use and its ability to be integrated with<br />
windows OS and Oracle Databases has made it a good choice for production line programming.<br />
Over the past year, JGI has created software that:<br />
• Checks barcodes for plate mix-ups<br />
• Checks the growth levels of glycerol stock wells<br />
• Sends emails when a robot fails<br />
• Uploads barcodes to an Oracle database.<br />
These pieces of software have become a valuable part of the sequencing facility and are used on a daily basis.<br />
The software has given the JGI the ability to better track its performance in key areas with information that was<br />
not previously collected because it would have been manually intensive.The software has also brought a new level<br />
of confidence and reliability to the JGI in terms of sequence assembly. By uploading associated plate pairs and<br />
preventing plate mix-ups the informatics team can focus on checking sequence matches instead of determining<br />
which projects have contaminated others. By catching mix-ups at the operator level, they have the chance to<br />
make notes in the database regarding source-destination pairs and reduce the amount of troubleshooting the<br />
informatics group has to do. This work was performed under the auspices of the US Department of Energy’s<br />
Office of Science, Biological and Environmental Research Program and by the University of California, Lawrence<br />
Livermore National Laboratory under Contract No. W-7405-Eng-48, Lawrence Berkeley National Laboratory under<br />
contract No. DE-AC03-76SF00098 and Los Alamos National Laboratory under contract No. W-7405-ENG-36.<br />
POSTER ABSTRACTS
WP082<br />
Hayley Wu<br />
Caliper Technologies Corp.<br />
Assay Development<br />
605 Fairchild Drive<br />
Mountain View, California 94043<br />
hayley.wu@calipertech.com<br />
Microfluidic Kinase Selectivity Screening Assays in Off-Chip Assay Format<br />
232<br />
Co-Author(s)<br />
Holly Reardon<br />
Thi Ngoc Vy-Trinh<br />
Ella Li<br />
Javier Farinas<br />
Jude Dunne<br />
Promising drug candidates are often abandoned due to toxic side effects. A biochemical surrogate of toxicity<br />
testing is evaluation of cross-reactivity with other related and unrelated targets. Kinase inhibitors pose an increased<br />
risk of cross-reactivity with other kinases, when the hit is a competitive inhibitor of ATP binding. A panel of >15<br />
kinases assays has been developed using Caliper’s off-chip mobility shift assay to screen for such unintended<br />
side effects. The extent of phosphorylation of a fluorescently labeled peptide was measured by electrophoretic<br />
separation of the product and substrate. All the assays were carried out in the same assay buffer, with the ATP<br />
concentration set at the Km for each kinase. The kinases selected, including CDK2, MAPKAPK2, and Fyn,<br />
comprised me<strong>mbers</strong> from all of the major kinase families. Dose response curves were measured for a series of 12<br />
commercially available kinase inhibitors. The assays were robust and reproducible (Z´ > 0.8) over an extended run<br />
time and from chip to chip. The observed pattern of activity of the inhibitors against the enzyme panel underscores<br />
the usefulness of selectivity screening early in the development of lead compounds.<br />
WP083<br />
John T. Y. Wu<br />
Government of Alberta<br />
Alberta Agriculture, Food and Rural Development<br />
O.S. Longman Laboratory Building<br />
6909 – 116 Street<br />
Edmonton, Alberta T6L 1X4 Canada<br />
john.wu@gov.ab.ca<br />
Co-Author(s)<br />
Eva Y. W. Chow<br />
Lester S. Y. Wong<br />
Evelyn E. Bowlby<br />
Development of an Automated High Throughput Screening Enzyme Linked Immunosorbent<br />
Assay for Chronic Wasting Disease Prions in Elk and Deer<br />
An automated high throughput-screening (HTS) enzyme linked immunosorbent assay (ELISA) was used to perform<br />
active surveillance for chronic wasting disease (CWD), a prion induced transmissible spongiform encephalopathy<br />
(TSE) found in elk and deer in Canada. It was developed from a licensed commercial diagnostic kit (BIO-RAD).<br />
In the United States this test has already been validated for detection of the abnormal prion protein (PrPres) for<br />
CWD. Our robot-assisted automated system is housed in a biosafety level II laboratory. Each tissue was assigned<br />
a 12-digit unique bar code number for specimen tracking and audit trail requirement according to ISO 17025.<br />
Homogenized tissues were treated with proteinase K at 37˚ C in microtubes or deep well microplates. Addition<br />
of butanol followed by centrifugation precipitated the PrPres and the resulting pellet was re-solublized, and its<br />
infectivity diminished by treatment at 100˚ C in a resolving buffer. A Biomek2000 or FX (Beckman Coulter) robotic<br />
liquid handling workstation linked with an ELx 405 microplate washer (Biotek) and a Vmax microplate reader<br />
(Molecular Device) was used to perform the sandwich ELISA. Computer programs were written to automate the<br />
ELISA protocol (liquid transfers, microplate washing, optical density values scoring, calculations and validation of<br />
the results). A 100% reproducibility and repeatability between the manual and automated-ELISA was achieved.<br />
A local area network was used for the analysis information management. By automating both the specimen<br />
preparation and ELISA, a 10-fold increase in output was seen. This system increases throughput, reduces labor<br />
cost and the overall unit cost of the test.
WP084<br />
Michael Yavilevich<br />
Inventor<br />
5 Haim Street Apt. 4<br />
Kiriat Bialik27076 Israel<br />
fastspin@barak-online.net<br />
Fast Spin Centrifuge, Analytical Module and Automated Laboratory System<br />
The present project relates to Rapid Centrifuge and compact Analytical Module like elements of Fast Automated<br />
Laboratory System. Fast Spin Technology conducts specimen separating and cap removing during same spin<br />
or use only cap removing step for previously separated tubes. Some new methods and devices were invented<br />
developing said Fast Spin Centrifuge and compact Analytical Module. Fast Spin realizes variable inclination spin<br />
method. This allows more rapid phase separation between serum, clot and gel. In a second stage the tubes<br />
spin while their longitudinal axes are aligned with the direction of the centrifugation force to allow reliable gel<br />
seal. Separation process in variable inclination tubes was investigated. This study allows at first to develop rapid<br />
Centrifuge, to continue in developing Pre-Analytical Station and complete in Analytical Module which aimed to<br />
solve the problem of modular aut<strong>omation</strong> of pre-analytical and analytical clinical laboratory processes. Fast Spin<br />
Technology is best suited to LaboratoryAut<strong>omation</strong>. Fast Spin is not only rapid separator. It is a powerful preanalytical<br />
and analytical station. This Technologysimplifies connecting tubes with Analyzer. Prototypes of the Rapid<br />
Centrifuge and cap removing device were built and successfully tested in some local laboratory. Preliminary results<br />
confirm the feasibility of the project. Invented new Analytical Module use Batch Technology for loading-unloading<br />
tubes, removal-replacement a caps, rapid centrifugation and specimen testing. This Technology intends to improve<br />
structure and productivity of all Clinical Laboratory Automated System. Fast service will bring new clients to these<br />
Labs. Fast Spin contains original technical and technological ideas and is in the global demand.<br />
WP085<br />
Lilly Zhang<br />
Amgen, Inc.<br />
Pharmacokinetics and Drug Metabolism<br />
One Amgen Center Drive MS 1-1B<br />
Thousand Oaks, California 91320<br />
leiz@amgen.com<br />
Quantitative Biological Sample Analysis Using NanoESI (Nanomate 100 ® ) –MS/MS<br />
233<br />
Co-Author(s)<br />
John D. Laycock<br />
Krys J. Miller<br />
The Nanomate 100 ® evaluation for quantitative analysis of exploratory pk screening is discussed. Calibration<br />
curves of Amgen compound A in plasma were analyzed on both Nanomate 100 ® –MS/MS and conventional<br />
LC-MS/MS. API3000 mass spectrometer was used in both cases. Results were compared on curve fitting, LLOQ,<br />
dynamic range, accuracy, precision, carryover, and matrix effect. The results show that Nanomate 100 ® performs<br />
comparably to LC-MS/MS. Nanomate 100 ® also exhibits advantages such as no cross contamination, minimal<br />
sample consumption, and much shorter sample cycle time. In addition, quantitation results of real study samples<br />
are shown. More than 100 plasma samples from various studies were analyzed on Nanomate 100 ® –MS/MS in<br />
parallel with LC-MS/MS. The results showed good correlation between the two interfaces. However, in many cases<br />
Nanomate ESI chip requires further sample clean up, and it can often be labor intensive. Additional advantages<br />
and limitations are also discussed. Overall, Nanomate 100 ® currently does not offer significant advantage in<br />
quantitative analysis of multiple analytes at sub-nanogram levels in early in vivo exploratory pk screening due to<br />
matrix suppression. However, Nanomate 100 ® demonstrates great potentials in multiple research areas such as<br />
Caco-2 screening, microsomal inhibition, and metabolite ID.<br />
POSTER ABSTRACTS
WP086<br />
Ruth Zhang<br />
Beckman Coulter, Inc.<br />
7451 Winton Drive<br />
Indianapolis, Indiana 46268<br />
ruth.zhang@sagian.com<br />
Plasmid Purification Using Promega’s Wizard* SV96 Reagents and Beckman Coulter’s<br />
Biomek ® 3000 Laboratory Aut<strong>omation</strong> Workstation<br />
234<br />
Co-Author(s)<br />
Chad Pittman<br />
Scott Boyer<br />
Laura Pajak<br />
The information included in this poster describes the utilization of a new liquid handler, the Biomek 3000<br />
Laboratory Aut<strong>omation</strong> Workstation, in the preparation of plasmid DNA using Promega’s Wizard SV 96 reagents.<br />
Using this system, plasmids are purified via binding to and eventual elution from a silica matrix using vacuum<br />
filtration. The following system components for the purification of plasmids will be described:<br />
• The new automated workstation, the Biomek 3000<br />
• The software and method to drive the workstation<br />
• The results when purifying plasmids using Wizard SV96 reagents.<br />
Representative data obtained from the purification of plasmids using this system will be shown. Data from<br />
automated capillary sequencing on Beckman Coulter’s CEQ 8000 Genetic Analysis System will be shown<br />
demonstrating the suitability of the purified plasmids for stringent assays such as capillary sequencing.<br />
WP087<br />
Juergen Zimmermann<br />
EMBL<br />
Genomics Core Facility<br />
Meyerhofstrasse 1<br />
69117 Heidelberg D69117 Germany<br />
zimmermann@embl-heidelberg.de<br />
Co-Author(s)<br />
Vladimir Benes, Christian Boulin, and Ralf Griebel<br />
Paul Lomax, Perkin Elmer Life Sciences<br />
Thomas Zinn, Ullrich Schübel, Macherey Nagel, and<br />
Klaus Günther Eberle, Hettich GmbH & Co KG<br />
Return of the Centrifuge: Automated DNA Extraction by Small Production Islands<br />
General liquid handling systems are well established and centrifuges are judged sometimes even as old fashioned<br />
in the field of aut<strong>omation</strong>. Nevertheless the combination of both systems is not wide spread in smaller systems.<br />
The combination of continuous and discontinuous processes is compromising either throughput or load balancing.<br />
Additional hardware is necessary for the interface of both instruments. Purification of DNA by Silica based methods<br />
is also a well established procedure which is automated nowadays with vacuum driven robotic systems. This<br />
aut<strong>omation</strong> approach is problematic under several circumstances. Especially sets of complex sample material (e.g.,<br />
animal tissues, plant tissues, forensic material) may overload conventional instruments. Variations in the viscosity<br />
of a single sample may ruin the output of a complete run, as the flow through is not guaranteed. The presented<br />
automated solution combines the stability of silica based purification chemistry and automated centrifugation,<br />
resulting in higher reproducibility and offering access to samples which couldn’t be processed so far.
NOTES<br />
235
NOTES<br />
236
INDUSTRY SPONSORED WORKSHOP LUNCHEONS<br />
Guests should have pre-registered through the company web sites. If you have not registered,<br />
please visit the company’s exhibit booth to inquire.<br />
Location Event<br />
Tuesday, February 3 12:00 – 1:30 pm<br />
Room J1 The Aut<strong>omation</strong> Partnership Workshop Lunch<br />
Room F Genetix Workshop Lunch<br />
Room A8 Greiner Bio-One Workshop Lunch<br />
Room C3 IDBS Workshop Lunch<br />
Room J4 Millipore Corporation Workshop Lunch<br />
Room N Tecan Workshop Lunch 1<br />
Room C2 Tecan Workshop Lunch 2<br />
Room J2 TekCel Workshop Lunch 1<br />
Room J3 Thermo Electron Workshop Lunch<br />
Location Event<br />
Wednesday, February 4 12:30 – 2:00 pm<br />
Room J4 Beckman Coulter Workshop Lunch<br />
Room F Caliper/Zymark Workshop Lunch<br />
Room A8 JALA Prospective Authors Workshop<br />
Room J1 MDL Information Services Workshop Lunch<br />
Room C2 Tecan Workshop Lunch 3<br />
Room J2 TekCel Workshop Lunch 2<br />
Room J3 Thermo Electron Workshop Lunch<br />
237<br />
INDUSTRY WORKSHOPS
Tuesday, February 3, 2004<br />
The Aut<strong>omation</strong> Partnership Workshop Lunch 12:00 – 1:30 pm Room J1<br />
The Aut<strong>omation</strong> Partnership<br />
3411 Silverside Road, Webster Building<br />
Wilmington, Delaware 19810<br />
(302) 478-9060; (302) 478-9575 fax<br />
info@aut<strong>omation</strong>partnership.com<br />
www.aut<strong>omation</strong>partnership.com<br />
Recent Advances in Aut<strong>omation</strong> for Cell-Based Assays – Growth, Selection, Optimization,<br />
and Quality 1536 Assays<br />
Generating and selecting new stable cell lines is labor intensive and time consuming. TAP is developing a flexible,<br />
modular system to automate parallel culture of thousands of cell lines, growing in multi-well plates. The system will<br />
significantly increase capacity for generating stable cell lines, remove this step as a bottleneck, and allow users to<br />
choose the optimum cell lines from many thousands.<br />
Genetix Workshop Lunch 12:00 – 1:30 pm Room F<br />
Genetix<br />
Queensway<br />
New Milton, Hampshire<br />
BH25 5NN United Kingdom<br />
info@genetix.com<br />
Advances in Microarraying From Genetix<br />
This workshop shows how you can implement the latest technology to improve the quality and throughput of your<br />
microarrays. Learn how you can print DNA or proteins on multiple surfaces, including into microplate wells. From<br />
sample prep to printing and scanning, see how industry leaders optimize their applications for maximum results.<br />
Whether you are a seasoned professional, or new to the technology, this is a must attend tutorial.<br />
Greiner Bio-One Workshop 12:00 – 1:30 pm Room A8<br />
Greiner Bio-One, Inc.<br />
1205 Sarah Street<br />
Longwood, Florida 32750<br />
(407) 333-2800; (407) 333-3001 fax<br />
info@us.gbo.com<br />
HTA-Plate: Unique 96-well plate for DNA- and Protein Arrays<br />
Within many biomolecular, pharmaceutical and diagnostic laboratories there is an increasing demand for quicker<br />
more cost-effective testing methods utilizing the most up to date techniques. Greiner Bio-One has now closed<br />
this gap with the development and launch of the HTAPlate (High-Throughput microArraying Plate). The “HTA<br />
Plate” combines the advantages of the universal microplate platform with microarrays for diagnostic applications.<br />
With 96 shallow wells and a low well rim of only 0.3 mm, the HTAPlate has been optimised for the simultaneous<br />
analysis of multiple samples. The low height of the rims facilitates both quick and cost effective printing of a wide<br />
range of analytes onto the 96-wells of the plate, and modern robotic spotters and arrayers are already equipped to<br />
handle this 96-well format.<br />
238
IDBS Workshop 12:00 – 1:30 pm Room C3<br />
IDBS<br />
2 Occam Court, Surrey Research Park<br />
Guildford GU2 7QB, United Kingdom<br />
44 (0) 1483 595 010; 44 (0) 1483 595 001 fax<br />
mstrangwick@id-bs.com<br />
Practical Methods and Business Rules for Automating Potency Data Processing<br />
This workshop will discuss business rules for processing potency data. Key questions that will be addressed<br />
include:<br />
QC of controls/standards<br />
• What calculations? • What limits?<br />
Fitting data to a model<br />
• Which model(s)? • What parameter settings?<br />
• Data point weighting/exclusion methods? • Allow extrapolation?<br />
• Significance level for activity? • What goodness-of-fit calculations and limits?<br />
• What combination of descriptors most<br />
completely describes activity?<br />
Assay Variability<br />
• What calculations? • What limits?<br />
The implementation and enforcement of business rules for high-throughput processing will also be considered.<br />
Millipore Corporation Workshop Lunch 12:00 – 1:30 pm Room J4<br />
Millipore Corporation<br />
290 Concord Road<br />
Billerica, Massachusetts 01821<br />
(800) 645-5476; (800) 645-5439 fax<br />
Starting From Scratch: Implementation of an Automated High Throughput Method for<br />
Determining Aqueous Compound Solubility in Drug Discovery<br />
There is growing awareness of the importance of measuring aqueous solubility in compound profiling, medicinal<br />
chemistry, and especially in conjunction with lead optimization. Methods that are currently available to measure<br />
solubility have insufficient throughput and may require too much compound to be useful for most screening and<br />
early ADME applications. Information will be presented that can be used to implement, automate, and validate<br />
a robust, reliable, and high throughput method for determining aqueous compound solubility based on the use<br />
of a new, 96-well device, MultiScreen* Solubility Filter Plate. Discussion topics will include sample requirements,<br />
detailed protocols, aut<strong>omation</strong> and analytical options, and the ability to perform on-line data acquisition and<br />
analysis. Data detailing assay performance, sample throughput, and correlation with established, low throughput<br />
methodologies will be presented.<br />
239<br />
INDUSTRY WORKSHOPS
Tecan Workshop Lunch 1 12:00 – 1:30 pm Room N<br />
Tecan<br />
4022 Stirrup Creek Drive, Suite 310<br />
Durham, North Carolina 27703<br />
(919) 361-5200; (919) 361-5201 fax<br />
info@tecan.com<br />
The Molecular Biology Experience With Tecan! (Novel Aut<strong>omation</strong> Solutions)<br />
In this session you will learn about some of the many solutions available from Tecan for the aut<strong>omation</strong> of cell and<br />
protein biology applications. Explore the potential for your lab to automate the entire spectrum of applications from<br />
gene to clone and beyond. We will present automated solutions for nucleic acid sample purification, PCR set-up,<br />
DNA transfection, large scale cell growth, cell lysis, protein purification, and protein crystaliography. You will learn<br />
about valuable cost-saving, space-saving, and time-saving solutions in the workshop to bring back to your lab.<br />
Tecan Workshop Lunch 2 12:00 – 1:30 pm Room C2<br />
Tecan<br />
4022 Stirrup Creek Drive, Suite 310<br />
Durham, North Carolina 27703<br />
(919) 361-5200; (919) 361-5201 fax<br />
info@tecan.com<br />
EVOware Software – Intuitive, Powerful, and Flexible!<br />
This workshop will introduce you to the simplified world of advanced instrument control. Tecan’s EVOware software<br />
package for the Freedom EVO platform is the next generation of liquid handling software. EVOware combines<br />
simplicity and ease of use with powerful features such as scheduling and the ability to integrate informatics<br />
solutions in a single software package. Come and have an opportunity to learn in depth about EVOware and have<br />
an opportunity to interact with our programmers.<br />
240
TekCel Workshop Lunch 1 12:00 – 1:30 pm Room J2<br />
TekCel, Inc.<br />
103 South Street<br />
Hopkinton, Massachusetts 01748<br />
(508) 544-7000; (508) 544-7001 fax<br />
info@tekcel.com<br />
Cherry Picking Redefined: Pick-List to Custom Assay Plate Generation on a New, Integrated<br />
Tube Management Platform<br />
Presenter: W. Steven Fillers, Ph.D.<br />
From genomics to high throughput screening; lead optimization to chemical profiling; on-demand retrieval and<br />
formatting of samples to support diverse discovery operations presents significant technical and logistical<br />
challenges. Efficient storage, retrieval and sorting of discrete samples and delivering them to the point of use in<br />
the desired format is laborious, time consuming, error prone and costly. This presentation will focus on a new<br />
Tube Management System, from TekCel, which provides superior throughput and process control to support<br />
the most demanding discovery applications. Individual 2-D bar coded tubes, or racks of tubes, are picked,<br />
sorted and compiled within the controlled storage environment of the TubeStore module. Tubes compiled to<br />
user specifications can be delivered for processing at rates up to 11,500 tubes (120 racks) per hour. Seamless<br />
integration to TekCel’s TubeServer, TekBench-SP and Plate Management System provides complete<br />
aut<strong>omation</strong> of sample processing tasks (96-channel tube piercing, controlled thawing, reformatting, master and<br />
assay plate generation) all within a controlled, inert processing environment. The system’s modular architecture<br />
affords considerable flexibility and provides a clear path for system expansion to accommodate future capacity,<br />
throughput or workflow needs.<br />
Thermo Electron Workshop Lunch 12:00 – 1:30 pm Room J3<br />
Thermo Electron<br />
5344 John Lucas Drive<br />
Burlington, Ontario L7L 6A6 Canada<br />
(905) 332-2000 x 319; (905) 332-1114 fax<br />
christy.jacobs@thermo.com<br />
The Tools to Upgrade: Designing Scalable Laboratory Aut<strong>omation</strong><br />
The reality in today’s research lab is change. How do you accommodate change while maintaining high<br />
throughput?<br />
Presented by Thermo Laboratory Aut<strong>omation</strong> and Integration, this workshop is about automating your laboratory<br />
with a modular platform that facilitates rapid change -- in assays and instrumentation. You will learn how to<br />
automate your assays using a new solution-focused Thermo layout tool. You will also learn how you scale<br />
aut<strong>omation</strong> from benchtop to production line.<br />
No materials are required, just think about your assay flow and where the bottlenecks are in your lab before<br />
coming to this workshop.<br />
241<br />
INDUSTRY WORKSHOPS
Wednesday, February 4, 2004<br />
Beckman Coulter Workshop Lunch 12:30 – 2:00 pm Room J4<br />
Beckman Coulter, Inc.<br />
4300 N. Harbor Boulevard<br />
Fullerton, California 92834<br />
The Use of Beckman Coulter’s Biomek ® Laboratory Aut<strong>omation</strong> Workstations for Proteomic<br />
Applications<br />
Presenters: Laura Pajak, Ph.D. and Michael Simonian, Ph.D.<br />
This workshop describes various techniques commonly used in proteomics research. We have broken down the<br />
processes involved into four main areas:<br />
• Sample Preparation (Centrifugation)<br />
• Protein Identification /Proteome Profiling (2D HPLC, MALDI TOF, PA 800)<br />
• Protein Characterization (DNA prep, His Tag purification, ELISA)<br />
• Protein Function (Cell Probes, Cell Preparation, Protein Protein interaction, Bacterial 2 hybrid system)<br />
We will discuss each of these areas and describe where the use of Beckman Coulter aut<strong>omation</strong> workstations<br />
(Biomek 2000, Biomek 3000, Biomek NX, Biomek FX) is beneficial. The use of aut<strong>omation</strong> to improve throughput,<br />
reproducibility and data quality will be described.<br />
Caliper/Zymark Workshop Lunch 12:30 – 2:00 pm Room F<br />
Caliper/Zymark<br />
605 Fairchild Drive<br />
Mountain View, California 94043<br />
(650) 623-0700; (650) 623-0500 fax<br />
info@calipertech.com<br />
Advances in Microfluidic Screening Assays<br />
Presenters: Walter Ausserer and Javier Farinas<br />
Microfluidic lab-on-a-chip screening assays have been developed for many target classes, including protein<br />
kinases, protein phosphatases, proteases, lipid kinases, and GPCR’s. In this workshop, we will present details<br />
of novel screening assays developed within the past year. Emphasis will be placed upon new assays for protein<br />
kinase selectivity screening and calcium flux. We also will preview Caliper’s next-generation microfluidic screening<br />
platform.<br />
JALA Prospective Authors Workshop 12:30 – 2:00 pm Room A8<br />
JALA Prospective Authors Workshop<br />
Presenter: Mark F. Russo, Ph.D., JALA Executive Editor<br />
Each year, ALA publishes six issues of JALA. The first half of this workshop focuses on how and why ALA<br />
me<strong>mbers</strong> can: showcase their work in this popular and highly regarded scientific journal, take advantage of JALA’s<br />
new online services for authors, and explore what volunteer opportunities exist for authors and other interested<br />
me<strong>mbers</strong>. The second half focuses on practical advice for preparing successful technical papers. Workshop<br />
is open to all conference attendees. For more information contact: Journal of the Association for Laboratory<br />
Aut<strong>omation</strong> (JALA), 819 Shorewood Boulevard, Manitowoc, WI 54220, (920) 652-0427, jala@labaut<strong>omation</strong>.org.<br />
242
MDL Information Services Workshop Lunch 12:30 – 2:00 pm Room J1<br />
MDL Information Services<br />
14600 Catalina Street<br />
San Leandro, California 94577<br />
(510) 895-1313; (510) 614-3608 fax<br />
l.hill@mdl.com<br />
Inventory Management Solution for Discovery Scientists<br />
Learn to manage your sample and plate inventory with MDL Plate Manager and interface with chemistry and<br />
biology data. The workshop will demonstrate how to create, dilute, pool, remap, and store plates; browse the<br />
inventory and drill down to sample and compound level details; track volumes, freeze/thaw cycles, locations, and<br />
each plate’s parents and daughters. See how the inventory data connects to the chemical registration database<br />
to access compound batch information, and share information with MDL Assay Explorer for a complete data<br />
management solution.<br />
Tecan Workshop Lunch 3 12:30 – 2:00 pm Room C2<br />
Tecan<br />
4022 Stirrup Creek Drive, Suite 310<br />
Durham, North Carolina 27703<br />
(919) 361-5200; (919) 361-5201 fax<br />
info@tecan.com<br />
The Molecular Biology Experience With Tecan! (Integrating and Validating Our Partners Kits)<br />
In this exploratory session, you will learn about work done with our reagent partners to produce automated<br />
solutions for molecular biology labs. Tecan partners with many leading molecular biology reagent companies to<br />
provide the most flexible, open platform for molecular biology aut<strong>omation</strong>. Come and hear our partners talk about<br />
automating their kits on Tecan aut<strong>omation</strong>.<br />
Together, Tecan and our partners can provide you with proven solutions for all aspects of your molecular biology<br />
aut<strong>omation</strong> needs: from gene to clone and beyond! Come learn more in this session.<br />
243<br />
INDUSTRY WORKSHOPS
TekCel Workshop Lunch 2 12:30 – 2:00 pm Room J2<br />
TekCel, Inc.<br />
103 South Street<br />
Hopkinton, Massachusetts 01748<br />
(508) 544-7000; (508) 544-7001 fax<br />
info@tekcel.com<br />
Stability of Compounds Stored in TekCel Plate Management System.<br />
Application of New Approaches for Ultra-High-Throughput Analysis of Compound Stability<br />
Presenter: James Connelly, Ph.D.<br />
Collections of compounds used for pharmaceutical lead generation are both a major asset and an opportunity<br />
for drug discovery. Therefore, protection and preservation of this high-value resource is of supreme importance.<br />
Stability of compounds stored in DMSO solutions can be increased by application of conditions such as inert<br />
atmosphere and low temperature. The TekCel Plate Management System has been used for over 1.5 years to store<br />
combinatorial libraries at the Aventis Combinatorial Technologies Center. Increased surveillance and continuous<br />
QC of stored collections can be facilitated by new technology for ultra-high-throughput chromatography and<br />
mass spectrometric analysis. Preliminary results on the application of new analytical technologies and the relative<br />
stability of these drug discovery samples will be presented.<br />
Thermo Electron Workshop Lunch 12:30 – 2:00 pm Room J3<br />
Thermo Electron<br />
5344 John Lucas Drive<br />
Burlington, Ontario L7L 6A6 Canada<br />
(905) 332-2000 x 319; (905) 332-1114 fax<br />
christy.jacobs@thermo.com<br />
Thermo Electron’s KingFisher ® – A Novel High Throughput Platform for Protein<br />
Purification Applications<br />
Thermo will be presenting a flexible customer solution for recombinant protein purification based on magnetic<br />
particles. This solution combines Thermo’s unique KingFisher purification technology and Dynal Biotech’s<br />
Dynabeads ® TALON ® , offering rapid protein purification with high-quality results. Purified proteins are ready for<br />
use in a variety of downstream applications such as screening for new drugs and drug targets and studying DNAprotein<br />
or protein-protein interactions.<br />
Fully functional high-purity proteins can be isolated with excellent reproducibility and increased sensitivity.<br />
This robust and reliable method can purify up to 96 samples in less than 30 minutes. Data showing excellent<br />
performance of the new solution will be presented.<br />
244
NOTES<br />
245
EXHIBITOR LISTING<br />
3M Bioanalytical Booth 1219<br />
AB Controls Booth 1326<br />
ABgene Booth 1439<br />
ABS, Inc. Booth 1541<br />
Adept Technology, Inc. Booth 1528<br />
Adhesives Research, Inc. Booth 940<br />
Advion BioSciences, Inc. Booth 528<br />
Aerotech, Inc. Booth 1513<br />
Affordable Aut<strong>omation</strong> Ltd Booth 1532<br />
AID-CORP Booth 1615<br />
ALIO Industries Booth 1608<br />
Allegro Technologies Booth 1619<br />
American Linear Manufacturers Booth 1132<br />
Amersham Biosciences Corp. Booth 1010<br />
Applied Biosystems Booth 1520<br />
Applied Mechatronics Booth 1437<br />
Applied Robotics, Inc. Booth 233<br />
Applied Scientific Instrumentation Booth 1407<br />
Apricot Designs, Inc. Booth 1538<br />
ARTEL Booth 834<br />
Association for Laboratory Booth 1607<br />
Aut<strong>omation</strong><br />
Astech Projects Ltd. Booth 1424<br />
Aurora Discovery, Inc. Booth 909<br />
Axygen Scientific Booth 1426<br />
Bal Seal Engineering Booth 1324<br />
Barnstead International/STEM Booth 808<br />
BD Biosciences Booth 1319<br />
Beckman Coulter, Inc. Booth 1107<br />
Big Bear Aut<strong>omation</strong> Booth 240<br />
Bio-Tek Instruments, Inc. Booth 908<br />
Bio Integrated Solutions Booth 1503<br />
Bi<strong>omation</strong> Booth 912<br />
BioMedTech Laboratories, Inc. Booth 113<br />
BioMicroLab, Inc. Booth 241<br />
Biosero Booth 1536<br />
Biotage Booth 215<br />
BioTX Aut<strong>omation</strong>, Inc. Booth 111<br />
Bishop-Wisecarver Corporation Booth 1227<br />
BMG Labtechnologies, Inc. Booth 707<br />
Boston Biomedica, Inc. Booth 1220<br />
Brandel Booth 213<br />
Brinkmann Instruments Inc. Booth 632<br />
Brooks Automaton, Inc., Booth 1228<br />
Life Sciences Group<br />
Caliper Technologies Corporation Booth 1201<br />
The Center for Biophysical Booth 235<br />
Sciences and Engineering<br />
Chromagen, Inc. Booth 1141<br />
Code Refinery Booth 932<br />
Computype, Inc. Booth 1519<br />
Corning Incorporated Booth 312<br />
CyBio AG Booth 338<br />
deCODE genetics Booth 836<br />
Del-Tron Precision Inc. Booth 107<br />
Digital Bio Technology, Inc. Booth 711<br />
DigitalVAR, Inc. Booth 837<br />
Dionex Corporation Booth 534<br />
Discovery Partners International Booth 1008<br />
DRD Diluter Corporation Booth 1433<br />
Drug Discovery & Development Booth 135<br />
Drug Discovery World Booth 1610<br />
Eastern Plastics, Inc. Booth 1037<br />
E&K Scientific Products Booth 1113<br />
EDC Biosystems Booth 1515<br />
Eksigent Technologies Booth 833<br />
ELMO Motion Control, Inc. Booth 1537<br />
Elsevier Booth 1607<br />
Encynova Booth 727<br />
Essen Instruments, Inc. Booth 332<br />
Evotec Technologies Booth 937<br />
Exatron Corporation Booth 1507<br />
FIBERLite Centrifuge Booth 1118<br />
Fisher Scientific Booth 1038<br />
G&L Precision Die Cutting, Inc. Booth 1509<br />
General Data Company, Inc. Booth 1033<br />
Genetic Engineering News/ Booth 725<br />
Modern Drug & Discovery<br />
Genetix Booth 425<br />
Genmark Aut<strong>omation</strong> Booth 539<br />
Genomic Solutions Booth 810<br />
GenoVision Inc. Booth 1535<br />
GenVault Corporation Booth 541<br />
Gilson, Inc. Booth 1025<br />
Glas-Col, LLC Booth 210<br />
Greiner Bio-One, Inc. Booth 619<br />
Hamilton Company Booth 207<br />
HEMCO Corporation Booth 421<br />
Hettich Booth 933<br />
Hudson Control Group, Inc. Booth 1413<br />
IDBS Inc. Booth 439<br />
IKO International, Inc. Booth 1125<br />
ILS Innovative Labor Systeme Booth 1229<br />
INA Linear Technik, Booth 1318<br />
A division of INA USA Corp.<br />
Innovadyne Technologies, Inc. Booth 733<br />
Innovative Microplate Booth 814<br />
Invetech Instrument Development Booth 435<br />
and Manufacture<br />
ISC BioExpress Booth 936<br />
J-Kem Scientific, Inc. Booth 1006<br />
Jencons Scientific, Inc. Booth 713<br />
Jouan Robotics Booth 507<br />
Julabo USA, Inc. Booth 520<br />
Jun-Air, USA Booth 1134<br />
Kendro Laboratory Products Booth 1524<br />
246<br />
Kloehn Company Booth 533<br />
KMC Systems, Inc. Booth 1514<br />
LABCON North America Booth 1129<br />
Labcyte Booth 1136<br />
LabVantage Solutions, Inc. Booth 1221<br />
Lathrop Engineering, Inc. Booth 1425<br />
Lawrence Berkeley Booth 941<br />
National Laboratory<br />
LEAP Technologies Booth 1036<br />
Liconic Instruments Booth 206<br />
Los Alamos National Laboratory Booth 824<br />
Luminex Corporation Booth 735<br />
Macherey-Nagel Booth 709<br />
MATECH Booth 934<br />
MatriCal, Inc. Booth 1328<br />
Matrix Technologies Corporation Booth 1506<br />
MDL Information Systems Booth 1112<br />
MéCour Temperature Control Booth 1120<br />
Merlin BioProducts BV Booth 1600<br />
Mettler-Toledo Autochem Booth 535<br />
Micralyne, Inc. Booth 1032<br />
MicroGroup Booth 906<br />
Micronic Systems Booth 441<br />
Micropump, Inc. Booth 519<br />
Microscan Systems, Inc. Booth 1427<br />
Millipore Corporation Booth 907<br />
MJ Research, Inc. Booth 1014<br />
Modern Drug Discovery/ Booth 109<br />
Chemical & Engineering News<br />
Molecular BioProducts Booth 1007<br />
Molecular Devices Corporation Booth 719<br />
Motoman, Inc. Booth 1212<br />
Multi-Contact USA Booth 1238<br />
MWG Biotech, Inc. Booth 137<br />
Nanostream Booth 232<br />
National Instruments Booth 1511<br />
NB Corp of America Booth 1035<br />
New England Small Tube Booth 1329<br />
Corporation<br />
NSK Precision America, Inc. Booth 1011<br />
NuGenesis Technologies Booth 715<br />
NUNC Brand Products Booth 532<br />
Opticon, Inc. Booth 939<br />
Oriental Motor USA Corp. Booth 1612<br />
Orochem Technologies, Inc. Booth 1041<br />
Oyster Bay Pump Works, Inc.. Booth 1428<br />
Pall Life Sciences Booth 1411<br />
Parker Hannifin Corporation Booth 636<br />
PerkinElmer Life and Booth 625<br />
Analytical Sciences<br />
PharmaGenomics Magazine Booth 841<br />
Pierce Biotechnology, Inc. Booth 1218
EXHIBIT HALL<br />
San Jose McEnery Convention Center<br />
141 240<br />
139 238<br />
137 236<br />
135 234<br />
133 232<br />
115<br />
212<br />
113<br />
111 210<br />
109<br />
206<br />
107<br />
241<br />
338<br />
239<br />
237 336<br />
235 334<br />
233 332<br />
213 312<br />
215<br />
211<br />
POSTERS<br />
207<br />
441<br />
538<br />
439<br />
536<br />
435<br />
534<br />
433 532<br />
520 421<br />
419 518<br />
407<br />
528<br />
425<br />
524<br />
539 636<br />
535<br />
632<br />
533<br />
525<br />
519<br />
618<br />
620<br />
Pneutronics Division of Booth 739<br />
Parker Hannifin Corporation<br />
Point Technologies, Inc. Booth 1409<br />
Popper & Sons, Inc. Booth 419<br />
Porvair Sciences Ltd. Booth 1518<br />
Process Analysis and Aut<strong>omation</strong> Booth 1533<br />
Promega Corporation Booth 1320<br />
Protedyne Corporation Booth 525<br />
RAPP POLYMERE GmbH Booth 1606<br />
REMP AG Booth 913<br />
ReTiSoft, Inc. Booth 334<br />
Rheodyne LLC Booth 521<br />
Richard Scientific, Inc. Booth 234<br />
Rigaku Booth 536<br />
Rixan Associates Booth 1401<br />
RoboDesign International, Inc. Booth 538<br />
Roche Applied Science Booth 524<br />
Roche Instrument Center Ltd. Booth 1429<br />
RSF Electronics, Inc. Booth 1601<br />
RTS Life Science International Booth 1119<br />
SAGE Publications Booth 239<br />
Sanyo Scientific Booth 806<br />
SCIENCE/AAAS Booth 832<br />
Scientific Specialties, Inc. Booth 935<br />
541<br />
507<br />
625<br />
735<br />
834<br />
733 832<br />
729<br />
826<br />
727<br />
725 824<br />
619 719<br />
ENTRANCE<br />
840<br />
739<br />
836<br />
841 940<br />
839 938<br />
837 936<br />
835 934<br />
833 932<br />
941<br />
1038<br />
939<br />
937 1036<br />
935 1034<br />
933 1032<br />
247<br />
1035 1134<br />
1033 1132<br />
1025<br />
1021 1120<br />
1019 1118<br />
714 814<br />
912<br />
1014<br />
913<br />
1015 1114<br />
713<br />
810<br />
711<br />
811<br />
908<br />
1008<br />
909<br />
1112<br />
1011<br />
1110<br />
709 808<br />
807<br />
1007 1106<br />
707 806<br />
906 907 1006<br />
1041 1141 1240<br />
1541 1640<br />
1136<br />
1037<br />
1139 1238<br />
1439 1538<br />
1539 1638<br />
1437 1536 1537 1636<br />
1133<br />
1129 1228<br />
1127<br />
1224<br />
1125<br />
1220<br />
1119<br />
1218<br />
1113 1212<br />
1107<br />
Scinomix, Inc. Booth 1106<br />
Scivex Booth 1419<br />
Seyonic SA Booth 1127<br />
SGE, Inc. Booth 1015<br />
Shimadzu Biotech Booth 1139<br />
Sias Booth 236<br />
Silicon Valley Scientific, Inc. Booth 115<br />
Silex Microsystems AB Booth 237<br />
SKF USA Inc. Booth 141<br />
SLR Systems, Inc. Booth 133<br />
SMAC Booth 1539<br />
Sorenson BioScience Booth 336<br />
Spark Holland, Inc. Booth 620<br />
Sparton Medical Solutions Booth 618<br />
SPEX CertiPrep Booth 1325<br />
Stäubli Corporation Booth 1240<br />
Tecan/Tecan Systems, Inc. Booth 407<br />
Technical Manufacturing Booth 1114<br />
Corporation<br />
TekCel, Inc. Booth 1101<br />
TEKnova Booth 518<br />
TeleChem Int/ArrayIt.com Booth 840<br />
The Aut<strong>omation</strong> Partnership Booth 1224<br />
The Lee Company Booth 1225<br />
1229 1328<br />
1227 1326<br />
1225 1324<br />
1221 1320<br />
1219 1318<br />
1329 1428<br />
1327 1426<br />
1325 1424<br />
1319<br />
1101 1201 1401<br />
1534 1535 1634<br />
1433<br />
1532 1533 1632<br />
1429 1528<br />
1427<br />
1524<br />
1425<br />
1419<br />
1518<br />
1520<br />
1514<br />
1413<br />
1411<br />
1506<br />
1409<br />
1407<br />
1501<br />
1628<br />
1525 1626<br />
1624<br />
1620<br />
1519<br />
1618<br />
1515 1614<br />
1513 1612<br />
1511 1610<br />
1509 1608<br />
1507 1606<br />
1503 1602<br />
1501 1600<br />
1641<br />
1639<br />
1637<br />
1635<br />
1633<br />
1629<br />
1627<br />
1625<br />
1621<br />
1619<br />
1615<br />
1607<br />
1603<br />
1601<br />
ALA<br />
BOOTH<br />
Thermo Electron Booth 507<br />
THK America Booth 212<br />
Titertek Instruments Booth 1021<br />
Titian Software Ltd. Booth 835<br />
Tomtec Booth 811<br />
Torcon Instruments, Inc. Booth 211<br />
TradeWinds Direct, Inc. Booth 1327<br />
Tricontinent Booth 1019<br />
TTP LabTech Booth 1525<br />
Union Biometrica, Inc. Booth 938<br />
United Chemical Technologies, Inc. Booth 1501<br />
USA Scientific, Inc. Booth 729<br />
V&P Scientific, Inc. Booth 826<br />
Veeco Instruments inc. Booth 1012<br />
Velocity11 Booth 1133<br />
VICI Valco Instruments Booth 807<br />
Waters Corporation Booth 1110<br />
Watson-Marlow Bredel Pumps Booth 839<br />
Whatman Booth 433<br />
White Carbon Booth 1034<br />
Zinsser Analytic GmbH Booth 1534<br />
EXHIBITORS
Featuring the World’s Largest Exhibition on Laboratory Aut<strong>omation</strong><br />
• A full spectrum of emerging and enabling laboratory technologies<br />
• More than 300 leading laboratory aut<strong>omation</strong> product and services providers<br />
• Today’s top biotechnology companies, equipment manufacturers, and more<br />
Stay on the cutting edge of laboratory technology at LabAut<strong>omation</strong>2004, you can expect unique opportunities to<br />
learn about the latest and greatest in laboratory aut<strong>omation</strong> products, services, and solutions. LabAut<strong>omation</strong>2004<br />
has the most expansive laboratory aut<strong>omation</strong> exhibition in the world and guaranteed to keep you on the cutting<br />
edge of science.<br />
You’ll see firsthand what’s new in the field of laboratory aut<strong>omation</strong><br />
from today’s leading biotechnology companies, scientific equipment<br />
manufacturers, and much more.<br />
Not only will LabAut<strong>omation</strong>2004 help you keep up – it will propel you<br />
into a more exciting future!<br />
To purchase exhibition space for:<br />
February 1-5, 2004<br />
San Jose McEnery<br />
Convention Center<br />
San Jose, California<br />
248<br />
Location: Exhibit Hall<br />
Days & Hours:<br />
Monday, February 2, 2004 4:00 - 8:00 pm<br />
Tuesday, February 3, 2004 10:00 am - 6:30 pm<br />
Wednesday, February 4, 2004 10:00 am - 6:30 pm<br />
ALA LabFusion 2004<br />
Hynes Convention Center<br />
Boston, Massachusetts<br />
ALA_offi ce@labaut<strong>omation</strong>.org<br />
labaut<strong>omation</strong>.org<br />
Short Courses: June 12 – 13, 2004<br />
Conference and Exhibition: June 13 – 16, 2004<br />
ALA LabFusion 2004 is ALA’s brand new conference and exhibition. This new conference complements<br />
LabAut<strong>omation</strong>, ALA’s highly successful west-coast event. ALA LabFusion 2004 will focus on<br />
biopharmaceutical development and applications, as well as practical implementations, including technology<br />
acquisitions, pharmaceutical technologies, process chemistry, and system validation and qualifications.<br />
LabAut<strong>omation</strong>2005<br />
San Jose McEnery Convention Center Short Courses: January 30 – 31, 2005<br />
San Jose, California Conference and Exhibition: January 31 – February 3, 2005<br />
Call or write:<br />
Space Reservation!<br />
ALA Event Management Office Daphne Glover<br />
343 W. Erie, Suite 330 Exhibit Sales & Sponsorships<br />
Chicago, Illinois 60610 (312) 654-4314; (312) 803-1927 fax<br />
dglover@labaut<strong>omation</strong>.org
3M Bioanalytical<br />
3M Center, Building 270-2A-08<br />
Maplewood, Minnesota 55144<br />
(651) 736-1426; (651) 736-1519 fax<br />
arichie-willits@mmm.com<br />
www.3m.com/empore<br />
AB Controls<br />
192 Technology Drive, Suite J<br />
Irvine, California 92618<br />
www.abcontrols.com<br />
ABgene<br />
565 Blossom Road<br />
Rochester, New York 14610<br />
www.abgene.com<br />
ABS, Inc.<br />
701-4 Cornell Business Park<br />
Wilmington, Delaware 19801<br />
(302) 654-4492; (302) 654-8046 fax<br />
services@absbio.com<br />
www.absbio.com<br />
Adept Technology, Inc.<br />
3011 Triad Drive<br />
Livermore, California 94551<br />
(925) 245-8109<br />
info@adept.com<br />
www.adept.com<br />
Booth 1219 (10 x 10)<br />
For high throughput ADME and Biological Sample Prep applications, Empore cards<br />
provide a simple route to high throughput sample drug screening. Activation, loading, and<br />
extraction for 96 samples can be accomplished in approximately one-half hour or less.<br />
The SPE card features 96 elution zones that can be rapidly loaded and eluted using newly<br />
developed equipment from Tomtec. Sequential sampling and direct injection into a mass<br />
spectrometer eliminate steps, saving customers time and money for basic drug discovery<br />
sample prep.<br />
Empore 96 well plates are ideal for sample prep in drug discovery and clinical studies.<br />
Empore SPExpress cards extend SPE to high throughput ADME and Early Drug<br />
Discovery Applications. Empore Affi nity Plates bind proteins, peptides, ligands, antigens,<br />
and antibodies for protein therapeutic and protein drug target studies.<br />
Booth 1326 (10 x 10)<br />
AB Controls is a Laboratory Aut<strong>omation</strong> Solution Provider. We emphasize on small and<br />
medium size robotic mass storage solutions for microplates, Tip Racks and nested tips.<br />
Products:<br />
1. Trx: 96 and 384 nested tip delivery system: 80 and 160 rack models available.<br />
2. Apix: 4 axis robot for delivery of microplates and tip racks.<br />
3. PlateSpace: A variable size, random access plate Storage/Retrieval system.<br />
Other custom solutions can be made.<br />
Booth 1439 (10 x 20)<br />
ABgene, ® an Apogent company, produce a diverse range of molecular biology reagents,<br />
plastic consumables and instrumentation, enabling us to offer complete product solutions.<br />
We provide local support and keep in close contact with the research community through<br />
our network of international sales offi ces and UK headquarters. To ensure that we continue<br />
to meet your expectations, we have invested heavily in research, both in-house and<br />
through collaborations with universities and industrial partners. We have also continued<br />
to expand our manufacturing facilities, which includes a state-of-the-art injection<br />
moulding cleanroom and laboratories. Our aim is to build on our strengths of quality,<br />
competitiveness, innovation and responsiveness. We are confi dent that our commitment<br />
to science and new technologies will enable us to offer you the best products and service<br />
now, and in the future.<br />
Booth 1541 (10 x 10)<br />
Analytical Biological Services Inc. (ABS) provides customized Bioreagents, Cell Culture,<br />
and RNA to drug discovery laboratories in nearly all the major pharmaceutical companies<br />
throughout the world. These preparations include: cell culture scale-up, Baculovirus,<br />
receptor binding preparations, enzyme extracts, Total RNA and Poly A+ RNA.<br />
Booth 1528 (10 x 10)<br />
Adept Technology is America’s largest manufacturer of industrial robots with more than<br />
30,000 factory aut<strong>omation</strong> systems installed worldwide. Adept manufactures a broad<br />
range of standard and cleanroom robots at work in the Laboratory Aut<strong>omation</strong> and<br />
Medical Device Manufacturing industry performing microtiter plate handling, colony<br />
picking, medical device assembly, surgical kit assembly and suture handling. Adept<br />
products can be used to automate a variety of applications in this industry including<br />
MicroTiter Plate Handling, Automated Sample Processing, Colony Picking, Medical Device<br />
Assembly, Material Handling and Packaging, Vision-Based Part Feeding and Machine<br />
Load/Unload – Injection Molding.<br />
249<br />
EXHIBITORS
Adhesives Research, Inc.<br />
400 Seaks Run Road<br />
Glen Rock, Pennsylvania 17327<br />
(800) 445-6240; (717) 235-8320 fax<br />
bday@arglobal.com<br />
www.adhesivesresearch.com<br />
Advion BioSciences, Inc.<br />
15 Catherwood Road<br />
Ithaca, New York 14850<br />
(607) 266-0665; (607) 266-0749 fax<br />
boardmaa@advion.com<br />
www.advion.com<br />
Aerotech, Inc.<br />
101 Zeta Drive<br />
Pittsburgh, Pennsylvania 15238<br />
www.aerotech.com<br />
Affordable Aut<strong>omation</strong> Ltd<br />
Unit 21, The Bridgewater Centre<br />
Robson Avenue<br />
Urmston<br />
Manchester M41 7TE England<br />
0044 161 747 1890; 0044 161 747 1891 fax<br />
sales@affordableaut<strong>omation</strong>.co.uk<br />
www.affordableaut<strong>omation</strong>.co.uk<br />
AID-CORP<br />
Analytical and Industrial Development<br />
P.O. Box 4405<br />
Salem, Massachusetts 01970<br />
(978) 745-6265, (978) 745-6376 fax<br />
www.aid-corp.com<br />
ALIO Industries<br />
2339 West 8th Street<br />
Loveland, Colorado 80537<br />
(970) 461-9902; (970) 461-8828 fax<br />
sales@alioindustries.com<br />
www.alioindustries.com<br />
Booth 940 (10 x 10)<br />
Adhesives Research designs and manufacturers custom high-performance, pressuresensitive<br />
adhesive tapes and coated fi lm products for the healthcare industry. AR tape<br />
systems meet defi ned design criteria for microfl uidic devices and microplate sealing<br />
tapes. Specifi c technologies including low fl uorescence systems, conductive adhesives,<br />
structural systems, adhesives that withstand rapid thermal cycling processes, and<br />
systems that exhibit hydrophilic/hydrophobic surface characteristics. Four separate cGMP<br />
manufacturing facilities (Glen Rock, PA and Limerick, Ireland) are dedicated to adhesive<br />
formulating, coating, laminating, slitting, and fi nishing.<br />
Booth 528 (10 x 10)<br />
Advion BioSciences’ NanoMate 100 is the fi rst fully automated nanoelectrospray<br />
system for mass spectrometry. This novel microchip-based analytical technology<br />
signifi cantly enhances the data quality, utility and speed of electrospray ionization (ESI)<br />
mass spectrometry and eliminates carryover. The heart of the solution is the ESI Chip,<br />
a micro array of nozzles etched in silicon. The NanoMate allows laboratories to improve<br />
the performance of their mass spectrometers for proteomics and small molecule drug<br />
discovery and development.<br />
Booth 1513 (10 x 10)<br />
Motion Control and Positioning System Leader<br />
Aerotech has well over 30 years of experience as a supplier of top quality motion control<br />
products and positioning systems for the medical industry. Aerotech’s products have<br />
been successfully deployed in medical applications such as DNA & Proteomics research,<br />
drug discovery, hermetic seam welding, laser cutting of stents, intraocular lenses, hip<br />
replacement joints, and general medical process aut<strong>omation</strong>.<br />
Booth 1532 (10 x 10)<br />
Manchester based Affordable Aut<strong>omation</strong> has, for more than a decade, been at the<br />
forefront of developments in laboratory technology, building automated cells for some of<br />
the leading pharmaceutical organizations in the United Kingdom and Europe. The key to<br />
Affordable Aut<strong>omation</strong>’s success is in the understanding of the tightly controlled processes<br />
by today’s laboratories. Working closely with scientists, Affordable Aut<strong>omation</strong> develops<br />
bespoke aut<strong>omation</strong> systems to manage ultra High Throughput Screening, plate stacking<br />
and handling, vial fi lling and clean room cuff testing for companies such as AstraZeneca,<br />
Organon, Celltech, and Pfi zer.<br />
Booth 1615 (10 x 10)<br />
AID-CORP specializes in helping labs expand their budgets through improved technology<br />
management. AID-CORP provides quality reconditioned instrumentation and services<br />
to the Laboratory community. AID-CORP offers high-quality instrumentation including<br />
the following: Laboratory Aut<strong>omation</strong>/Robotics Systems; Microplate readers/washers<br />
and Spectrophotometers, PCR Thermal Cyclers, Scintillation Counters, Chromatography<br />
and Mass Spec systems, NMR’s and other high-end lab instrumentation We also offer<br />
the following services: Technology Valuations & Assessments, Equipment Remarketing<br />
Services We can meet with you to discuss how we can tailor an approach which meets<br />
your needs. Call Phil or Victoria Jackson at (978) 745-6265 for more information.<br />
Booth 1608 (10 x 10)<br />
ALIO Industries designs and manufactures precision aut<strong>omation</strong> serial stages and parallel<br />
kinematic robots for nanometer resolution and repeatability applications. Custom and<br />
standard motion solutions in metrology, microscopy, micro machining, micro assembly,<br />
fi ber optic alignment, laboratory equipment, and medical equipment for end user and OEM<br />
applications in atmospheric, clean room and vacuum environments.<br />
250
Allegro Technologies<br />
Unit 8<br />
Enterprise Centre<br />
Pearse Street<br />
Dublin 2 Ireland<br />
353 1 6791464; 353 1 6791544<br />
devin@allegro-technologies.com<br />
www.allegro-technologies.com<br />
American Linear Manufacturers<br />
629 Main Street<br />
Westbury, New York 11590<br />
(800) 892-3991; (516) 333-1729 fax<br />
sales@americanlinear.com<br />
www.americanlinear.com<br />
Amersham Biosciences Corp<br />
800 Centennial Avenue<br />
P.O. Box 1327<br />
Piscataway, NJ 08855-1327<br />
(800) 526-3593 or (732) 457-8000;<br />
(877) 295-8102 fax<br />
www.amershambiosciences.com<br />
Applied Biosystems<br />
3833 North First Street<br />
San Jose, California 95134<br />
www.appliedbiosystems.com<br />
Applied Mechatronics<br />
800 Antiquity Drive<br />
Fairfield, California 94534<br />
(707) 207-0587; (509) 356-8623 fax<br />
glenn@appliedmechatronics.com<br />
www.appliedmechatronics.com<br />
Booth 1619 (10 x 20)<br />
Allegro Technologies is a global leader in the development and production of reliable,<br />
robust and user friendly liquid handling solutions, enabling the addition and the transfer of<br />
nanoliter and microliter volumes of a wide range of fl uids.<br />
Allegro’s proprietary non-contact spot-on liquid handling technology brings signifi cant<br />
improvements to existing assays by increasing levels of precision and accuracy as well as<br />
decreasing assay costs through use of lower volume pipetting.<br />
Allegro will exhibit the Equator NS 808 eight channel pipetting system and will also<br />
feature the newest version of the system which incorporates a new bulk dispensing mode<br />
as well as traditional aspirate dispense mechanism further increasing the fl exibility of the<br />
system as a total solution provider.<br />
Booth 1132 (10 x 10)<br />
American Linear Manufacturers features “Made in USA” Precision Crossed Roller Linear<br />
Bearing motion products. ALM Crossed roller bearings are characterized by smooth,<br />
noiseless, frictionless motion, long life and zero side play in any attitude. On display at<br />
LabAut<strong>omation</strong>2004 will be automated single and multiple axis (XY, XYZ) positioning<br />
stages with Micro-stepping control system, custom designed motorized and manual<br />
positioning stages and a full display of ALM standard product line including motor ready<br />
positioning stages, slides, bearings, and components. Stop by and see our sleek, medical<br />
grade stainless steel/nickel plated aluminum stages. New low cost linear motion stages<br />
available.<br />
Booth 1010 (10 x 10)<br />
Amersham Biosciences Corp, the life science business of Amersham plc (LSE, NYSE,<br />
OSE: AHM), is a world leader in developing and providing integrated systems and<br />
solutions for disease research, drug development and manufacture. Our systems are used<br />
to uncover the function of genes and proteins, for the discovery and development of drugs<br />
and for the manufacture of biopharmaceuticals.<br />
Booth 1520 (10 x 10)<br />
Applied Biosystems, a business unit of Applera Corporation, develops and markets<br />
instrument-based systems, reagents, analytical software, informatics solutions, and<br />
contract services to the life science industry. The company aims to provide complete<br />
informatics solutions to customers in basic research, drug discovery, and development<br />
and offers a set of core software functionality with a world-class professional service<br />
organization to create whole lab solutions for better quality, throughput, and compliance.<br />
Booth 1437 (10 x 10)<br />
Applied Mechatronics is a manufacturer’s representative fi rm whose aim is to provide<br />
customers access to some of the nation’s top suppliers of motion control components and<br />
systems. With several decades of accumulated experience in automated machine design,<br />
our strengths in mechanical and electronic aut<strong>omation</strong> allow us to offer “out-of-the-box”<br />
expertise related to how these products interact with each other and in the real world.<br />
Furthermore, our expertise in working with multiple industries allows us to offer a crosspollination<br />
of ideas for your mechatronic needs.<br />
251<br />
EXHIBITORS
Applied Robotics, Inc.<br />
648 Saratoga Road<br />
Glenville, New York 12302<br />
(518) 384-1000; (518) 384-1200 fax<br />
info@arobotics.com<br />
www.arobotics.com<br />
Applied Scientific Instrumentation<br />
29391 West Enid Road<br />
Eugene, Oregon 97402<br />
(541) 461-8181; (541) 461-4018 fax<br />
info@asiimaging.com<br />
www.asiimaging.com<br />
Apricot Designs, Inc.<br />
825 S. Primrose Avenue Unit i<br />
Monrovia, California 91016<br />
(626) 256-6088; (626) 256-6060 fax<br />
info@apricotdesigns.com<br />
www.apricotdesigns.com<br />
ARTEL<br />
25 Bradley Drive<br />
Westbrook, Maine 04092<br />
www.artel-usa.com<br />
Association for Laboratory<br />
Aut<strong>omation</strong><br />
3N866 Ferson Creek Road<br />
St. Charles, Illinois 60174<br />
(866) 263-4928; (312) 803-1927 fax<br />
ala_offi ce@labaut<strong>omation</strong>.org<br />
labaut<strong>omation</strong>.org<br />
Astech Projects Ltd.<br />
Unit 4 Berkeley Court,<br />
Manor Park<br />
Runcorn<br />
Cheshire<br />
WA7 1TQ<br />
United Kingdom<br />
44 (0)1928 571797; 44 (0)1928 571162 fax<br />
peter.greenhalgh@astechprojects.co.uk<br />
www.astechprojects.co.uk<br />
Booth 233 (10 x 10)<br />
Applied Robotics, Inc. has a reputation for quality and reliability in the design and<br />
manufacture of aut<strong>omation</strong> accessories, including; QuickSTOP Collision Sensors,<br />
QuickConnect Systems, XChange Tool Change Systems, Single Axis Force Sensors,<br />
Grippers, Rotary Actuators and Laboratory Aut<strong>omation</strong> Accessories. Applied Robotics,<br />
Inc. is an international company serving the worlds aut<strong>omation</strong> market. Our products and<br />
services are used widely in manufacturing, welding, medical, food processing, assembly,<br />
and material handling markets. Applied Robotics, Inc. is an employee owned company<br />
and ISO9001 registered.<br />
Booth 1407 (10 x 10)<br />
ASI manufactures hardware for laboratory aut<strong>omation</strong> and fl uorescence microscopy<br />
that include: extremely precise closed-loop X Y Stages & DC drives for ultra-precise<br />
Z-axis focusing and XY-axis positioning. These precision devices can be used for<br />
microspotting, microarrays, microfabrication, and a number of other nanotechnology<br />
applications. ASI also offers high-speed fi lter wheels, monochromators and shuttering<br />
devices, microinjectors and micromanipulators, and a wide range of other devices for<br />
micropositioning and illumination control including complete photometry and imaging<br />
systems.<br />
Booth 1538 (10 x 20)<br />
At Apricot Designs, we are proud to call ourselves specialists in multi-channel microvolume<br />
pipettors, disposable pipet tips, and high-performance solvent evaporators. The<br />
primary focus of the company has been on multi-channel micro volume fl uid pipetting<br />
technology for high throughput and drug discovery applications. Our latest innovations<br />
include the unique 384 channel pipettors which not only work with 384 and 1536-well<br />
labware, but can also pipette 96-well labware! This convenient downward compatibility<br />
is one of its kind in the present market! In addition to our design and development<br />
capabilities, we also are supported with our in-house machine shop. This allows us to<br />
quickly respond to the needs of our customers for any custom equipment or equipment<br />
modifi cations.<br />
Booth 834 (10 x 10)<br />
The new Artel MVV Calibration System (Multi-Channel Volume Verifi cation) uses a novel<br />
dual-dye photometric method to overcome the limitations of other methods designed to<br />
calibrate automated liquid handling equipment. System components include equipment<br />
and reagents. Also featuring the Artel PCS ® Pipette Calibration System used for calibrating<br />
manual pipettes and Artel Pipette Tracker software, which provides automatic electronic<br />
data management of pipettes and their performance.<br />
Booth 1607 (10 x 40)<br />
The Association for Laboratory Aut<strong>omation</strong> is a worldwide organization representing<br />
leaders in all aspects of laboratory aut<strong>omation</strong>. ALA seeks to provide a greater<br />
understanding of the importance and value of aut<strong>omation</strong> technologies in laboratory<br />
settings, to advance science and promote education related to laboratory aut<strong>omation</strong><br />
by encouraging the study, advancing the science and improving the practice of medical<br />
and laboratory aut<strong>omation</strong>. For more information, visit ALA in booth 1607, log on to<br />
labaut<strong>omation</strong>.org, or contact ALA at (866) 263-4928.<br />
Booth 1424 (10 x 10)<br />
ASTECH Projects create bespoke aut<strong>omation</strong> systems for each customer’s unique<br />
requirements. ASTECH are specialists in a number of key areas such as Inhaler and<br />
Respiratory aut<strong>omation</strong>, High Throughput Experimentation (H.T.E) and novel “never<br />
been done before” solutions. ASTECH also integrate existing semi-automated systems<br />
(i.e. autosamplers, powder dispensers and specialist laboratory equipment) to produce<br />
truly innovative solutions that are admired throughout the industry. ASTECH deliver<br />
aut<strong>omation</strong> solutions that are both robust and fi t for purpose. Our key customers are in the<br />
Pharmaceutical, Life Sciences, Food and Petro-Chemical industries.<br />
252
Aurora Discovery, Inc.<br />
9645 Scranton Road, Suite 140<br />
San Diego, California 92121<br />
(858) 334-4500; (858) 334-4564 fax<br />
info@auroradiscovery.com<br />
www.auroradiscovery.com<br />
Axygen Scientific<br />
33170 Central Avenue<br />
Union City, California 94587<br />
(510) 494-8900; (510) 494-0700 fax<br />
bob@axygen.com<br />
www.axygen.com<br />
Bal Seal Engineering<br />
19650 Pauling<br />
Foothill Ranch, California 92610-2610<br />
(800) 366-1006 or (949) 460-2100<br />
(949) 460-2300<br />
sales@balseal.com<br />
www.balseal.com<br />
Barnstead International/STEM<br />
2555 Kerper Boulevard<br />
Dubuque, Iowa 52001-1478<br />
www.barnstead.com<br />
BD Biosciences<br />
2 Oak Park<br />
Bedford, Massachusetts 01730<br />
(800) 343-2035 or (978) 901-7300<br />
(800) 743-6200 or (978) 901-7493 fax<br />
labware@bd.com<br />
www.bdbiosciences.com<br />
Beckman Coulter, Inc.<br />
4300 N. Harbor Boulevard<br />
Fullerton, California 92834<br />
www.beckmancoulter.com<br />
Booth 909 (10 x 20)<br />
Aurora Discovery, Inc. is an international leader in the development of products and<br />
technologies that allow pharmaceutical companies to select and develop new medicines<br />
more rapidly and cost-effectively. Located in San Diego, we provide high value solutions to<br />
research challenges through automated instrumentation and software.<br />
Booth 1426 (10 x 10)<br />
Leading manufacturer of quality plastic consumables and lab equipment with focus on the<br />
HTS, PCR and general laboratory consumable market. LabAut<strong>omation</strong>2004 emphasis on<br />
Maxymum Recovery technology of tips, tubes and plates. Also, introducing a table top Heat<br />
Sealer for use in sealing adhesive fi lms to plates. Many new products: PCR style plates,<br />
Deep Well plates, reservoirs used in HTS, and many new robotic tip styles. Distributors<br />
located in over 80 countries worldwide. Known for exceeding the quality standards and<br />
performance of OEM manufacturers, for superior service and competitive pricing.<br />
Booth 1324 (10 x 10)<br />
For OEMs and suppliers with precision spring and seal needs, Bal Seal Engineering is the<br />
knowledgeable, professional and responsive engineering and manufacturing solutions<br />
provider of high quality, high value sealing, connecting, and energizing components<br />
worldwide.<br />
Since inception in 1958, pioneering, creating, and vision have been the keywords in<br />
describing the Bal Seal Engineering dynamic. Today, with over four decades of groundbreaking<br />
achievements and more than 120 active patents, Bal Seal Engineering designs,<br />
manufactures, and supplies customized products for sealing and innovative canted-coil<br />
springs for electrical current transfer and grounding; EMI shielding; and mechanical<br />
holding, latching, and loading applications.<br />
Booth 808 (10 x 10)<br />
STEM, a division of Barnstead International is a manufacturer of synthesis equipment for<br />
the drug discovery and development market. With a full range of synthesis equipment from<br />
reaction blocks to fully automated liquid handling workstations, STEM|ReactArray products<br />
are powerful tools for process development, crystallization, and degradation.<br />
Booth 1319 (20 x 20 Island)<br />
BD Biosciences, a business segment of BD (Becton, Dickinson and Company), has four<br />
units: Clontech, Discovery Labware, Immunocytometry Systems, and Pharmingen. As<br />
one of the largest companies supporting the life sciences, BD Biosciences provides<br />
integrated, high-value products and services for genomics, proteomics, drug discovery<br />
and development, and cell analysis. Product offerings include innovative BD Falcon<br />
plasticware for tissue culture, fl uid handling and high throughput screening, a unique line<br />
of BD BioCoat cultureware with preapplied extracellular matrix and cell culture reagents<br />
that include ECM proteins, cytokines, and media additives.<br />
Booth 1107 (20 x 30)<br />
At Beckman Coulter, we’re streamlining your discovery process by bringing together<br />
essential components, and making them work together for you. Stop by our booth and<br />
come see what’s new in the world of laboratory workstations and learn about the new<br />
applications that are available to fi t your needs. Advanced technology combined with world<br />
class service and support are just another way our Smart Solutions are working for you.<br />
253<br />
EXHIBITORS
Big Bear Aut<strong>omation</strong><br />
Pleasanton, California 94566<br />
(510) 333-4338; (925)397-3148 fax<br />
www.bigbearaut<strong>omation</strong>.com<br />
Bio-Tek Instruments, Inc.<br />
Highland Park, P.O. Box 998<br />
Winooski, Vermont 05404<br />
(888) 451-5171<br />
(902) 655-7941 fax<br />
customercare@biotek.com<br />
www.biotek.com<br />
Bio Integrated Solutions<br />
15 E. Palatine Road, Suite 107<br />
Prospect Heights, Illinois 60070<br />
(847) 325-5104; (847) 325-5105 fax<br />
mary@biointsol.com<br />
www.biointsol.com<br />
Bi<strong>omation</strong><br />
15091 AL Highway 20<br />
Madison, Alabama 35756<br />
(888) 297-4710; (256) 353-1774 fax<br />
info@bi<strong>omation</strong>.net<br />
www.bi<strong>omation</strong>.net<br />
BioMedTech Laboratories, Inc.<br />
6408 East Fowler Avenue<br />
Tampa, Florida 33617-2404<br />
(813) 985-7180; (813) 985-7957 fax<br />
jsasse@biomedtech.com<br />
www.biomedtech.com<br />
BioMicroLab, Inc.<br />
2500 Dean Lesher Drive, Suite A<br />
Concord, California 94520<br />
(925) 689-1200; (925)689-1263 fax<br />
info@biomicrolab.com<br />
www.biomicrolab.com<br />
Booth 240 (10 x 10)<br />
The Microplate Orbital Shaker product line from Big Bear Aut<strong>omation</strong> is a leading high<br />
quality shaker used in biotechnology, genomics, proteomics, and lab applications. This line<br />
of single wellplate 1mm orbital shakers provides the smallest foot print, highest speeds<br />
for vortexing, and the lowest temperature range on the market, and includes a lab model<br />
and an RS232 data driven model. Big Bear Aut<strong>omation</strong> also provides complex industrial<br />
aut<strong>omation</strong> machinery and robotics for a variety of industries.<br />
Booth 908 (10 x 20)<br />
Since the introduction of our fi rst product in 1981, Bio-Tek’s Laboratory Division has<br />
emerged as a worldwide leader in microplate instrumentation. Today, Bio-Tek designs,<br />
manufactures and distributes a full line of microplate instrumentation including<br />
absorbance, fl uorescence, and luminescence readers for multi-well formats; microplate<br />
washers for 96-/384-well applications; automated liquid handling and dispensing<br />
instruments; and data reduction software that expands every application from research to<br />
clinical market.<br />
Booth 1503 (10 x 10)<br />
Specializing in scalable and versatile liquid handling systems, value-added instrumentation<br />
and database support, Bio Integrated Solutions provides leading-edge laboratory aut<strong>omation</strong><br />
and device integration solutions to the bench-top scientist as well as to core facilities. The<br />
compact and fl exible BISFlex liquid handling systems and the BioSpotter line of spotters<br />
provide comprehensive data logging and seamless interfacing with external devices through a<br />
powerful user-friendly software framework. Reactor blocks, Peltier devices, shakers, balances,<br />
plate sealers, evaporators and interface devices are available as standalone devices or as part<br />
of a custom integrated system.<br />
Booth 912 (10 x 20)<br />
Bi<strong>omation</strong> Life Sciences Group provides process driven or turnkey automated systems to<br />
the life sciences industry. Our life sciences process knowledge and engineering expertise,<br />
in combination with our industrial quality aut<strong>omation</strong> experience and comprehensive<br />
programming capability, yields reliable, cost effective solutions using cutting-edge,<br />
proven, aut<strong>omation</strong> and robotic technology for your manufacturing, clinical, clean<br />
room or laboratory process aut<strong>omation</strong> needs. Services provided include evaluation,<br />
recommendation, simulation and implementation. These are followed up by on-site training,<br />
and service and maintenance for our dedicated, 24-hour, on call aftermarket group.<br />
Booth 113 (10 x 10)<br />
BioMedTech Laboratories, Inc., manufactures a line of high-quality 96-, 384-, and 1536well<br />
microplates, custom-coated with streptavidin, biotin, protein A/G/L, anti rabbit/mouse/<br />
goat/human antibodies, Ni-chelate, glutathione, reactive amino/carboxyl/maleimidegroups,<br />
non-binding surface coatings, oligonucleotides, polylysine, collagen, fi bronectin<br />
or basement membrane extracellular matrix. BioMedTech also produces a panel of higheffi<br />
ciency blocking reagents engineered for inactivating the surface of plastic, glass, metal<br />
or silicon to maximize signal-to-noise ratio in receptor-, immuno-, kinase-, DNA/RNA,<br />
microarray- and biochip assays.<br />
Booth 241 (10 x 10)<br />
BioMicroLab’s XL20 tube handling system automates tube sorting for standard 96 tube<br />
rack labware. The XL20 robotics feature built-in 2D scanner technology to track 2D marked<br />
tubes. BioMicroLab offers several 2D tube scanner systems for single tube or 96 tube<br />
rack applications. Visit BioMicroLab to see the latest in automated tube handlers and 2D<br />
scanning systems for 96 tube rack labware.<br />
254
Biosero<br />
16909 Parthenia Street, Suite 102<br />
North Hills, California 91343<br />
(818) 891-7666; (818) 891-7336 fax<br />
info@bioseroinc.com<br />
www.bioseroinc.com<br />
Biotage<br />
P.O. Box 8006<br />
Charlottesville, Virginia 22932<br />
(800) 446-4752; (434) 979-4743 fax<br />
info@biotage.com<br />
www.biotage.com<br />
BioTX Aut<strong>omation</strong>, Inc.<br />
16753 Donwick, Suite A6<br />
Conroe, Texas 77385<br />
(936) 273-2633; (936) 273-2633 fax<br />
www.biotxaut<strong>omation</strong>.com<br />
Bishop-Wisecarver Corporation<br />
2104 Martin Way<br />
Pittsburg, California 94565<br />
(925) 439-8272; (925) 439-5931 fax<br />
sales@bwc.com or info@bwc.com<br />
www.bwc.com<br />
BMG Labtechnologies, Inc.<br />
2415 Presidential Drive<br />
Building 204, Suite 118<br />
Durham, North Carolina 27703-8026<br />
(919) 806-1735; (919) 806-8526 fax<br />
usa@bmglabtech.com<br />
www.bmglabtech.com<br />
Boston Biomedica, Inc.<br />
375 West Street<br />
W. Bridgewater, Massachusetts 02379<br />
(508) 580-1900; (508) 580-2202 fax<br />
lbraswell@bbii.com<br />
www.bbii.com<br />
Booth 1536 (10 x 10)<br />
Biosero brings together a consortium of businesses into a sales and support structure that<br />
provides an ideal communication path between our customers and our business partners.<br />
Biosero offers a range of products and services, from reagents and consumables to<br />
software to instrumentation, with a focus on laboratory aut<strong>omation</strong>.<br />
Booth 215 (10 x 10)<br />
Biotage manufactures equipment and consumables for purifi cation from drug discovery<br />
to production scale. The new compact Sp4 system for the medicinal chemist easily<br />
and reliably automates fl ash purifi cation, increasing productivity and freeing chemist from<br />
manual purifi cations tasks. The Sp4 system sequentially operates four FLASH cartridges,<br />
each with a dedicated sample collection rack and waste bottle. The recently launched<br />
Syntage product line includes functionalized chemistry media cartridges that eliminate<br />
manual work-up and integrate synthesis and purifi cation into one seamless operation.<br />
Other High Performance Flash Chromatography (HPFC) products include the Horizon,<br />
Horizon Pioneer, and the new Quad UV parallel purifi cation system, plus FLASH<br />
cartridges and Samplet cartridges. Biotage launched the new Flex V3 software.<br />
Booth 111 (10 x 10)<br />
BioTX Aut<strong>omation</strong> is a startup company specializing in the integration and development<br />
of standard and custom robotic end effecters and work stations for Epson SCARA<br />
robots. These work stations and end effecters are manufactured from ABS plastic utilizing<br />
BioTX’s patent pending Rapid Aut<strong>omation</strong> Development System (RADS). Current standard<br />
offerings include Agar plate manipulation, 96 & 384: well pipeting, reagent dispensing, and<br />
plate manipulation.<br />
Booth 1227 (10 x 10)<br />
Bishop-Wisecarver Corporation’s DualVee Motion Technology ® exhibits performance<br />
characteristics most often associated with medical equipment design—smooth, low noise,<br />
reliable antifriction guidance. Our product line includes linear systems, rotary guides and<br />
systems, stainless steel, high temperature and clean room compatible components, and<br />
aluminum machine framing. For more than 30 years, Bishop-Wisecarver Corporation has<br />
provided its patented DualVee ® guide wheel design to a host of aut<strong>omation</strong> equipment<br />
engineers.<br />
Booth 707 (10 x 10)<br />
BMG Labtechnologies is a global manufacturer of microplate measurement and handling<br />
systems for basic research and High Throughput Screening. We focus on microplate<br />
readers with a wide variety of optical detection systems in conjunction with integrated<br />
liquid handling equipment. Established in 1989, BMG Labtechnologies is headquartered<br />
in Offenburg Germany, located on the edge of the Black Forest. The backbone of our<br />
worldwide sales and support network is formed by four BMG Labtechnologies subsidiaries<br />
located in the United States, United Kingdom, France, and Australia.<br />
Booth 1220 (10 x 10)<br />
Pressure Cycling Technology (PCT) uses rapid cycles of high and low hydrostatic pressure<br />
to control biomolecules. A PCT-based Sample Preparation System is offered by Boston<br />
Biomedica for the safe, effi cient, and reproducible extraction of nucleic acids and proteins<br />
from a variety of cells and tissues, including those generally considered “hard-to-lyse.”<br />
255<br />
EXHIBITORS
Brandel<br />
8561 Atlas Drive<br />
Gaithersburg, Maryland 20877<br />
(800) 948-6506; (301) 869-5570 fax<br />
sales@brandel.com<br />
www.brandel.com<br />
Brinkmann Instruments Inc.<br />
One Cantiague Road<br />
Westbury, New York 11590<br />
(516) 334-7500; (516) 334-7521 fax<br />
info@brinkmann.com<br />
www.brinkmann.com<br />
Brooks Aut<strong>omation</strong>, Inc.,<br />
Life Sciences Group<br />
15 Elizabeth Drive<br />
Chelmsford, Massachusetts 01824<br />
(978) 262-4354; (978) 262-4202 fax<br />
larry.chin@brooks.com<br />
www.brooks.com<br />
Caliper Technologies Corporation<br />
605 Fairchild Drive<br />
Mountain View, California 94043<br />
(650) 623-0700; (650) 623-0500 fax<br />
sales.info@calipertech.com<br />
www.calipertech.com<br />
The Center for Biophysical<br />
Sciences and Engineering<br />
1530 Third Avenue South<br />
Birmingham, Alabama 35294-4400<br />
(205) 975-9590; (205) 934-2659 fax<br />
Logan@cbse.uab.edu<br />
www.cbse.uab.edu<br />
Booth 213 (10 x 10)<br />
This year, Brandel proudly features our smaller Cell Culture System and Plate Sealing<br />
System. The Cell Culture system is self-contained within a hood, automating plate<br />
coating, cell seeding/feeding and permeability studies. Our Sealers offer an adhesivebased<br />
solution for applying uniform seals for most plate confi gurations, heights, and<br />
compositions.<br />
Booth 632 (10 x 20)<br />
Brinkmann Instruments exhibit will feature new liquid handling workstations, which range in<br />
performance from automated 96-well and single tube pipetting to nucleic acid purifi cation<br />
applications. This new line of lab aut<strong>omation</strong> equipment, called epMotion is designed and<br />
manufactured by Eppendorf, a world leader in liquid handling and innovative molecular<br />
biology research products. In addition to the workstations, the exhibit will include our<br />
Nucleic Acid Purifi cation, PCR enzyme and reagent system offering for molecular biology<br />
applications.<br />
Booth 1228 (10 x 10)<br />
The Life Sciences Group of Brooks Aut<strong>omation</strong> introduces the Parallab 350, a fully<br />
automated, integrated workstation for performing high throughput nanoliter volume<br />
reactions in molecular biology applications. Target applications include Cycle Sequencing,<br />
PCR, SNP’s, and Genotyping. Brooks also develops custom engineered equipment<br />
solutions for genomics, drug discovery, proteomics, and chemistry laboratories.<br />
Booth 1201 (20 x 40)<br />
Caliper Technologies is a leading provider of microfl uidic technology and laboratory<br />
aut<strong>omation</strong> solutions serving the worldwide life science, biotechnology, and<br />
pharmaceutical industries. With the recent acquisition of Zymark Corporation, Caliper<br />
now offers a state-of-the-art comprehensive portfolio of microfl uidic, liquid handling, and<br />
laboratory aut<strong>omation</strong> products designed to accelerate drug discovery and development.<br />
The Caliper booth at LabAut<strong>omation</strong>2004 features the complete line of Caliper and Zymark<br />
platforms.<br />
Booth 235 (10 x 10)<br />
The CBSE is a leading structural biology center with established high-throughput<br />
capabilities in: cloning, protein expression and purifi cation, protein crystallization and<br />
structural determination, molecular biophysics and biocalorimetry, structure directed<br />
combinatorial chemistry, assay development and high-throughput drug screening. The<br />
CBSE also has an ISO 9001:2000 certifi ed Engineering division to support the CBSE<br />
scientists with the development of specialized research instruments for the Center’s high<br />
throughput robotic workstations and laboratories. The engineering group has grown to<br />
become a full-service organization able to provide both academic, government, NASA (12<br />
different experiment systems for 35 space missions on 54 fl ights) and industry customers<br />
with solutions ranging from technical guidance all the way to full turn-key systems and<br />
services for mechanical, electrical and software. The engineering group is staffed by<br />
37 engineers and technicians with collectively over 500 years of applied life sciences<br />
engineering and aerospace experience.<br />
256
Chromagen, Inc.<br />
10449 Roselle Street<br />
San Diego, California 92121<br />
(858) 558-1456; (858) 558-7166 fax<br />
info@chromagen.com<br />
www.chromagen.com<br />
Code Refinery<br />
2201 Candun Drive, Suite 101<br />
Apex, North Carolina 27523<br />
(919) 367-0003; (919) 367-9966 fax<br />
samird@code-refinery.com<br />
www.code-refinery.com<br />
Computype, Inc.<br />
2285 West County Road C<br />
St. Paul, Minnesota 55113<br />
(651) 633-0633; (651) 633-5580 fax<br />
info@computype.com<br />
www.computype.com<br />
Corning Incorporated<br />
45 Nagog Park<br />
Acton, Massachusetts 01720<br />
(978) 635-2200; (978) 635-2476 fax<br />
clswebmail@corning.com<br />
www.corning.com/lifesciences<br />
CyBio AG<br />
Goeschwitzer Str. 40<br />
Jena 07745 Germany<br />
49 36413510; 49 3641351409 fax<br />
info@cybio-ag.com<br />
www.cybio-ag.com<br />
Booth 1141 (10 x 10)<br />
Chromagen is a leader in providing enabling detection technologies for drug discovery and<br />
life science research. Our fl uorescent technology platforms incorporate molecular tools<br />
and detection probes into advanced assays for gene expression, kinases and GPCRs,<br />
accelerating drug discovery, and bioresearch. Chromagen manufactures assays, and<br />
provides screening and molecular biology services.<br />
Booth 932 (10 x 10)<br />
Code Refi nery delivers custom software development and software validation solutions<br />
to automate laboratory processes. From controlling robotic instruments to validating<br />
integrated systems, we have the expertise to produce fast, effective results for clients in<br />
pharmaceutical, clinical diagnostics, biotechnology, and other FDA-regulated industries.<br />
We work with clients through the entire project life cycle to ensure that all business goals<br />
are successfully achieved.<br />
Booth 1519 (10 x 20)<br />
Computype specializes in the unique identifi cation of labware items via bar code<br />
technology. We produce variable-information bar code labels, on-demand printing<br />
systems, bar code scanners, software, and print/apply systems for slides, tubes, vials, and<br />
microwell plates. We also develop customized interface solutions to help manage your<br />
data communication requirements. In lab aut<strong>omation</strong>, Computype has the experience you<br />
need to get the results you want.<br />
Booth 312 (10 x 20)<br />
Corning Incorporated is the major manufacturer of high-quality, high performance<br />
tools for high throughput screening, cell culture, genomics, and liquid handling. At<br />
LabAut<strong>omation</strong>2004, we will be highlighting our comprehensive line of microplates with<br />
modifi ed surfaces that provide for optimal performance of cell based and homogenous<br />
assays. In addition we will feature our newest products including our 96 HTS Transwell.<br />
Booth 338 (10 x 20)<br />
CyBio is a global life sciences enterprise with its headquarters in Jena, Germany, and<br />
offi ces all over the world. Being a company at the cutting edge of technology, CyBio<br />
develops, produces, and sells technology platforms for drug discovery. With more<br />
than 15 years of experience CyBio establishes new standards for precision, speed,<br />
and engineering with its hardware, software, and service products for the aut<strong>omation</strong><br />
of drug screening processes. The portfolio covers a broad range of excellent solutions<br />
for pharmaceutical and agrochemical research: liquid handling, plate handling, plate<br />
imaging, and data handling to achieve fully automated screening technologies, the key for<br />
innovative and effi cient ultra high-throughput screening (uHTS). CyBio is a global market<br />
leader in the fi eld of simultaneous pipetting technology.<br />
257<br />
EXHIBITORS
deCODE genetics<br />
7869 NE Day Road W.<br />
Bainbridge Island, Washington 98110<br />
(206) 780-8535; (206) 780-8549 fax<br />
emeraldproducts@decode.com<br />
www.decode.com/emeraldproducts<br />
Del-Tron Precision Inc.<br />
5 Trowbridge Drive<br />
Bethel, Connecticut 06801<br />
(203) 778-2727; (203) 778-2721 fax<br />
deltron@deltron.com<br />
www.deltron.com<br />
Digital Bio Technology, Inc.<br />
Seoul National University<br />
1304 Institute of Advanced<br />
Machinery Design<br />
Seoul 151-742 Korea<br />
82 28897139; 82 28852267<br />
admin@digital-bio.com<br />
www.digital-bio.com<br />
DigitalVAR, Inc.<br />
26212 Industrial Boulevard<br />
Hayward, California 94545<br />
(510) 782-7577; (510) 784-1344 fax<br />
info@digitalvar.com<br />
www.digitalvar.com<br />
Dionex Corporation<br />
1228 Titan Way<br />
P.O. Box 3603<br />
Sunnyvale, California 94088-3603<br />
(408) 737-0700; (408) 730-9403 fax<br />
marcom@dionex.com<br />
www.dionex.com<br />
Booth 836 (10 x 20)<br />
deCODE’s Emerald Products for Structural Biology are designed to ease the bottlenecks of<br />
Crystallography. Validated through our own research, these same proven systems are now<br />
available to you. Crystallography workstations, software, screens, and plates are available<br />
as separate components or as an integrated system to fi t your specifi c needs. To fi nd out<br />
more, visit us at deCODE.com/emeraldproducts.<br />
Booth 107 (10 x 10)<br />
Manufacturer of linear bearings including: ball slides, crossed roller slides, 1, 2, or 3 axis<br />
micrometer driven positioning stages, motor-ready lead screw driven stages, frictionfree<br />
air actuators, recirculating slide guides, crossed roller rail sets. Products range<br />
from subminiature slides to heavy duty positioning tables. Both straight line design and<br />
recirculating type. Modifi ed slides can be incorporated into your design.<br />
Booth 711 (10 x 10)<br />
Digital Bio Technology is one of the chief frontier fi rms in the Bio-Infra fi eld. Our company<br />
develops Solution Tools as well as experimental and medical diagnostic/analysis systems<br />
based on a new technology of Bio-MEMS, which combines MEMS (Micro Electro<br />
Mechanical System) technology and Bio technology organically. C-Chip is microchip type<br />
hemocytometer made of thermoplastics. It is exactly as precise as conventional glass<br />
hemocytometer, but extremely cheaper. * Disposable and not wear out. * Accurate with<br />
QC qualifi cation. * Easy to use without washing out and autoclave. * Safe without the<br />
chance to be exposed to infectious sample and drugs. C-reader is Automatic mocrochiptype<br />
hemocutometer. The C-Reader system is a fast alternative to conventional manual<br />
counting methods. The principle of the technique used in the C-Reader is the well known<br />
method of fl uorescence microscopy, implemented in a small, highly advanced fl uorescence<br />
microscope combined with a CCD camera and software-based image analysis. The<br />
excitation source is green laser. The emission fi lter removes all wavelengths from the light<br />
emitted from the sample except red fl uorescence light. The red fl uorescent light from the<br />
particles in the sample is focused onto the detector (CCD camera). Then image analysis<br />
program counts the red particles to represent the cell number in the sample.<br />
Booth 837 (10 x 10)<br />
DigitalVAR, Inc., is a premier Value Added Reseller (VAR), centrally located in the San<br />
Francisco Bay area. We specialize in providing computer systems, integration, and<br />
disk imaging services for Life Science instrument manufacturers and laboratories.<br />
We understand the special needs of the Life Science industry. Namely: Cost, quality,<br />
model stability, accurately repeatable delivery day in and day out, open purchase order<br />
procurement model; hardware integration and quality assurance.<br />
Booth 534 (10 x 10)<br />
Dionex develops, manufactures, and sells a broad range of instruments and consumables<br />
for IC, HPLC, capillary- and nano LC, and extraction. We offer a family of ion<br />
chromatography systems, including the ICS-2500, ICS-2000, ICS-1500 and ICS-1000;<br />
the Summit and inert BioLC ® HPLC systems; the LC Packings UltiMate system for<br />
proteomics; Chromeleon, ® chromatography management software; the MSQ mass<br />
spectrometric detector; and accelerated solvent extraction (ASE ® ) instruments. Also<br />
offered are a range of IC columns for anions and cations, and HPLC columns designed for<br />
specifi c applications like carbohydrate analysis, DNA and protein separations.<br />
258
Discovery Partners International<br />
9640 Towne Centre Drive<br />
San Diego, California 92121<br />
(858) 455-8600<br />
www.discoverypartners.com<br />
DRD Diluter Corporation<br />
83 Pine Street<br />
W. Peabody, Massachusetts 01960<br />
(978) 536-7062; (978) 536-7054 fax<br />
drddiluter@drddiluter.com<br />
www.drddiluter.com<br />
Drug Discovery & Development<br />
Reed Business Information<br />
301 Gibraltar Drive, Box 650<br />
Morris Plains, New Jersey 07950<br />
(516) 541-8566; (603) 250-0925 fax<br />
jboyce@reedbusiness.com<br />
www.dddmag.com<br />
Drug Discovery World<br />
39 Vineyard Path<br />
Mortlake, London, SW14 8ET,<br />
United Kingdom<br />
44 208 487 5656; 44 209 487 5666 fax<br />
damian@rjcoms.com<br />
www.ddw-online.com<br />
Eastern Plastics, Inc.<br />
110 Halcyon Drive<br />
Bristol, Connecticutt 06010<br />
(860) 314-2880; (860) 314-2888 fax<br />
sales@easternplastics.com<br />
www.easternplastics.com<br />
Booth 1008 (10 x 10)<br />
Discovery Partners International, Inc. (Nasdaq: DPII) is a world leader in drug discovery<br />
collaborations. DPI offers integrated services, products, and systems that span the drug<br />
discovery continuum, including target characterization, screening and targeted library<br />
design and synthesis, high throughput and high content screening, lead generation and<br />
optimization, gene expression analysis products, and protein crystallization. DPI has<br />
actively contributed to dozens of drug discovery collaborations, working on many of the<br />
most promising new biological target areas for the biotech and pharmaceutical industries<br />
around the world.<br />
Booth 1433 (10 x 20)<br />
DRD’s patented Differential or Dual Resolution Displacement pumps (most recently<br />
the CV1dur) are best known for providing the leading immunochemistry analyzers<br />
with unique wide range with precision. DRD has now miniaturized the technology as<br />
replacement syringes and as air pipettes. The Dual Resolution Syringe (DRS) can<br />
replace a conventional single resolution syringe, giving the system extraordinary aspiration<br />
resolution as fi ne as that of a 10 microliter syringe along with the volume and blowout<br />
power of a 1 mL syringe. The DRD Nanoblast is an air-fi lled pipette system that can<br />
pick up and transfer nanoliter volumes touchless. DRD thus endows venerable positive<br />
displacement technology with the DRD differential principle so that it can aspirate, blastoff<br />
touchless or dilute sub microliter and nanoliter samples—without any small seals and<br />
with ample excursion for fi ne metering—with matchless robustness and precision. New<br />
instruments and system capability for automated DRD microplate, microarray and spotting<br />
systems will be shown.<br />
Booth 135 (10 x 10)<br />
Drug Discovery & Development, a Reed Business Information publication, delivers<br />
insightful and timely coverage of the latest tools and technologies shaping the dynamic<br />
world of drug discovery research and development. Topics include the latest developments<br />
in HTS, genomics/proteomics, chemistry/pharmacology, cell-based technologies, and<br />
informatics. To obtain your FREE subscription visit www.dddmag.com.<br />
Booth 1610 (10 x 10)<br />
Drug Discovery World (DDW) is the world’s leading, truly global business review of<br />
drug discovery and development. Written by the world’s leading experts and aimed<br />
at a more senior audience across the various R&D disciplines, DDW discusses the<br />
latest technological and scientifi c advancements and the commercial implications of<br />
implementing these advancements.<br />
Booth 1037 (10 x 20)<br />
EPI is an ISO 9001:2000 registered company specializing in close-tolerance, complex<br />
machining of plastics. Tolerances to ±0.0002". CNC milling, drilling to 50:1 aspect ratio,<br />
tapping, turning, screw machining, polishing/fi nishing, bonding, welding, assembly,<br />
annealing. Design, engineering, material selection assistance. Extensive experience<br />
in medical, clinical diagnostics, surgical instruments, laboratory apparatus, test and<br />
measurement products, multi- and single-layer manifolds, fl uidics, electronics. EPI is<br />
the largest plastic machining company in the world and works with customers in several<br />
countries.<br />
259<br />
EXHIBITORS
E&K Scientific Products<br />
1085 Florence Way<br />
Campbell, California 95008<br />
(800) 934-8114; (408) 378-2611 fax<br />
www.eandkscientific.com<br />
EDC Biosystems<br />
871 Fox Lane<br />
San Jose, California 95131<br />
(408) 273-2300<br />
info@edcbiosystems.com<br />
www.edcbiosystems.com<br />
Eksigent Technologies<br />
2021 Las Positas Ct., Suite 161<br />
Livermore, California 94551<br />
(925) 960-8869; (925) 960-8867 fax<br />
info@eksigent.com<br />
www.eksigent.com<br />
ELMO Motion Control, Inc.<br />
1 Park Drive, Suite 12<br />
Westford, Massachusetts 01886<br />
www.elmomc.com<br />
Elsevier<br />
625 Walnut Street, Suite 300<br />
Philadelphia, Pennsylvania 19106<br />
(215) 238-8741; (215) 238-6462 fax<br />
Encynova<br />
557-C Burbank Street<br />
Broomfield, Colorado 80020<br />
(303) 465-4800<br />
www.encynova.com<br />
Booth 1113 (10 x 20)<br />
E&K Scientifi c has been serving the pharmaceutical and biomedical research industries<br />
for over 28 years. Our long-standing commitment to provide total customer satisfaction<br />
and quality products has earned us a solid reputation throughout the medical industry.<br />
Our focus on product solutions for automating processes, reducing costs and waste has<br />
led us to develop a diverse product offering. Product solutions include storage systems,<br />
liquid handling systems, microwell plates from 1536 well to 6 well, reservoirs, molecular<br />
biology products and a full line of pipet tips to fi t most automated liquid handling systems.<br />
In addition, we are actively adding new products to meet the ever-evolving demands of the<br />
research and production communities.<br />
Booth 1515 (10 x 10)<br />
EDC Biosystems provides leading edge Research & Development tools and services to the<br />
Life Science Industries. More specifi cally, we offer novel solutions for the Combinatorial<br />
Materials Sciences and Drug Discovery markets with our patented True Non-contact<br />
Technology for low-volume liquid compound handling.<br />
Booth 833 (10 x 10)<br />
Eksigent Technologies is launching the Express HPLC system—the fastest, highest<br />
resolution liquid chromatography system available. The Express HPLC system provides<br />
as much as a 5X increase in assay speed without a loss of resolution. The Express-800<br />
system offers 8 fully independent HPLC systems in one compact enclosure, providing up<br />
to a 40X increase in throughput versus conventional analytical HPLC systems. Stop by the<br />
Eksigent booth to see a single-channel Express-100 system separate 7 compounds in less<br />
than 30 seconds!<br />
Booth 1537 (10 x 10)<br />
ELMO Motion Control is one of the leading suppliers of compact servo amplifi ers and<br />
motion controllers for brushless and DC servomotors within the low power range. Founded<br />
in 1988, the company has gained several years of experience in industrial electronic design<br />
and manufacturing. Today, serving a large number of leading machine manufacturers in<br />
different industry segments; such as measuring/inspection machinery, life sciences and<br />
lab aut<strong>omation</strong>. ELMO has locations in Germany, the United States and Israel. On top of<br />
that, ELMO offers full drive solutions, a combination of complete servo motor and drive<br />
packages. For further information see the company web site: www.elmomc.com.<br />
Booth 1607 (10 x 40)<br />
Elsevier is proud to present The Journal of the Association for Laboratory Aut<strong>omation</strong><br />
(JALA). Elsevier is the world’s largest scientifi c, technical, and medical provider,<br />
publishing journals as well as books and secondary databases. JALA is the offi cial journal<br />
of the ALA. It is a multi-disciplinary international forum devoted to the advancement of<br />
technology in the laboratory.<br />
Booth 727 (10 x 10)<br />
Encynova provides fl uid control systems for precision dispensing and metering. Digitallycontrolled<br />
Travcyl Systems deliver the accuracy and repeatability required for automated<br />
processes. Pumping capability, fl uid measurement, and fl ow control are all in one compact<br />
module which does not require valves or calibration. Control from a PC or PLC via RS-485<br />
communication. Travcyl Systems are small enough to be robot-mounted and they<br />
integrate easily into manufacturing, lab, and OEM applications.<br />
260
Essen Instruments, Inc.<br />
3909 Research Park Drive, Suite 400<br />
Ann Arbor, Michigan 48108<br />
(734) 769-1600; (734) 769-7295 fax<br />
info@essen-instruments.com<br />
www.essen-instruments.com<br />
Evotec Technologies<br />
Schnackenburgallee 114<br />
Hamburg D-22525 Germany<br />
(919) 467-8733; 49 40560 81488 fax<br />
robert.rodewald@evotec-technologies.com<br />
www.evotec-technologies.com<br />
Exatron Corporation<br />
2842 Aiello Drive<br />
San Jose, California 95111-2154<br />
(408) 629-7600 or (800) EXATRON<br />
www.exatron.com<br />
FIBERLite Centrifuge<br />
422 Aldo Avenue<br />
Santa Clara, California 95054<br />
(408) 988-1103; (408) 988-1196 fax<br />
www.piramoon.com<br />
Fisher Scientific<br />
2000 Park Lane<br />
Pittsburgh, Pennsylvania 15275<br />
(800) 766-7000<br />
www.fishersci.com<br />
G&L Precision Die Cutting, Inc.<br />
1766 Junction Avenue<br />
San Jose, California 95112<br />
(408) 453-9400 or (800) 327-4553<br />
(408) 451-1199 or (800) 587-4288 faxes<br />
rperez@glprecision.com<br />
www.glprecision.com<br />
Booth 332 (10 x 10)<br />
Essen Instruments introduces Pipeline—a new generation of sterile dispensing equipment<br />
for cell culture media and reagents. The Pipeline Dispenser is to be used inside a biocabinet<br />
and designed to alleviate the tedious nature of manual pipetting operations. The<br />
Pipeline is fast, effi cient, and promotes consistent sterile technique.<br />
Booth 937 (10 x 10)<br />
Evotec Technologies, located in Hamburg, Germany is a wholly owned subsidiary<br />
of Evotec OAI, and is engaged in the development and manufacture of high quality<br />
instrument platforms. Evotec’s core FCS+ (Fluorescence Correlation Spectroscopy)<br />
technology is used primarily for biochemical binding assays, while other new technologies<br />
are focused on cell-based assays. Evotec’s Opera system represents the cutting edge<br />
in high-speed confocal microscopy offering the ability to screen cell-based assays in<br />
excess of 100,000 wells per day. Newly released, the Evotec Elektra system provides<br />
fully-automated selection of single cell clones based on their fl uorescence and imaging<br />
characteristics. Further advancements of Evotec’s core technology will be released in 2004<br />
offering bench-top FCS+ analysis for biochemical studies.<br />
Booth 1507 (10 x 10)<br />
Exatron began operation in May of 1974, as an integrator of Medical, Semiconductor, and<br />
Industrial applications. We have developed a personal philosophy regarding our customers<br />
and products. We provide hard-working, well made, reasonably priced equipment<br />
designed to match our customers’ varying applications. Our customers enjoy the widest<br />
variety of system confi gurations possible. Exatron is totally committed to supporting our<br />
older products.<br />
Booth 1118 (10 x 10)<br />
FIBERLite Centrifuge’s Mission is to improve laboratory safety worldwide by replacing<br />
metallic centrifuge rotors with safer, stronger and lighter Carbon Fiber Rotors. FIBERLite<br />
will display a full range of carbon fi ber composite rotors and the new FIBERFuge, the<br />
world’s fi rst totally composite centrifuge. Please visit us at www.piramoon.com.<br />
Booth 1038 (10 x 20)<br />
World-leader in serving science for the life science, biomedical, pharmaceutical, safety,<br />
university, and chemical markets, enabling scientifi c discovery and clinical laboratory<br />
testing by offering over 600,000 products and services to over 350,000 customers in 145<br />
countries. One-stop source of research, healthcare, safety, fi re fi ghting, and controlled<br />
environment products, state-of-the-art e-commerce capabilities and integrated global<br />
logistics network services.<br />
Booth 1509 (10 x 10)<br />
G&L Microplate Liddings – microplate sealing tapes offer a variety of temperature<br />
tolerant, semi-fl exible and impermeable adhesive coated fi lms for use in laboratory<br />
applications; available in aluminum, polyolefi n, and clear or white polyester fi lms. G&L<br />
can also customize sealing tapes to meet specifi c application needs. G&L specializes in<br />
tight tolerance die cutting, printing and multi-layer lamination utilizing a variety of pressure<br />
sensitive adhesive materials for medical components, diagnostic components, medical<br />
packaging and medical electronics.<br />
261<br />
EXHIBITORS
General Data Company, Inc.<br />
4354 Ferguson Drive<br />
Cincinnati, Ohio 45245<br />
(800) 733-5252; (513) 752-6947 fax<br />
medinfo@general-data.com<br />
www.general-data.com<br />
Genetic Engineering News/<br />
Modern Drug & Discovery<br />
2 Madison Avenue<br />
Larchmont, New York 10538<br />
(914) 834-3100; (914) 834-3689 fax<br />
nrivera@liebertpub.com<br />
www.genengnews.com<br />
Genetix<br />
Queensway<br />
New Milton, Hampshire<br />
BH25 5NN United Kingdom<br />
info@genetix.com<br />
www.genetix.com<br />
Genmark Aut<strong>omation</strong><br />
1201 Cadillac Ct.<br />
Milpitas, California 95035<br />
(408) 678-8510; (408) 678-8595 fax<br />
rhiannonh@genmarkaut<strong>omation</strong>.com<br />
www.genmarkaut<strong>omation</strong>.com<br />
Genomic Solutions<br />
17851 Sky Park Circle<br />
Irvine, California 92887<br />
www.genomicsolutions.com<br />
Booth 1033 (10 x 10)<br />
General Data provides innovative solutions for barcode labeling and identifi cation to the<br />
healthcare industry. We will be featuring StainerShield – a new solution that brings<br />
effective on-demand pre-stainer bar code labeling of tissue and specimen slides to the<br />
lab. StainerShield labels are formulated to withstand the harsh chemicals of a laboratory’s<br />
slide staining and preparation process. They are easily printed in the lab, and can use data<br />
directly from the LIS. Complete label printing starter kits are available for under $3,000.<br />
www.stainershield.com<br />
Booth 725 (10 x 10)<br />
The most widely read publication in the biotechnology/bioprocess industry worldwide.<br />
Provides major coverage of signifi cant issues, novel drug discovery technologies,<br />
regulatory, and scaleup guidelines, R&D, fi nancial news (including public offerings,<br />
mergers, and venture capital), funding and government news, corporate profi les, university<br />
news, meeting reports, foreign reports, new product and literature information, and critical<br />
in-depth articles relating to the production of biotechnology products. Indexed in BIOSIS,<br />
Biotechnology Citation index (ISI), Research Alert (ISI), and SCiSearch/Science Citation<br />
Index- Expanded (ISI).<br />
Booth 425 (10 x 30)<br />
Genetix designs and manufactures automated systems to meet your Proteomics,<br />
Genomic, Cell Biology, and Liquid Handling application needs. Our instrumentation,<br />
consumables and reagents provide the total solution to your Microarraying, 2D Gel<br />
Excision and Colony Picking research.<br />
Booth 539 (10 x 10)<br />
Genmark Aut<strong>omation</strong> would like you to experience a new way of looking at automated<br />
plate handling with our introduction of the most advanced high throughput robotic and<br />
aut<strong>omation</strong> systems on the market. Now you have a choice! Genmark Aut<strong>omation</strong> has<br />
been a Total Aut<strong>omation</strong> Solutions provider of integrated aut<strong>omation</strong> systems for the<br />
global semiconductor, data storage and fl at panel display industries for over 15 years. With<br />
an install base of over 25,000 systems worldwide, Genmark continues to offer the most<br />
advanced, high throughput, fully customizable atmospheric and vacuum robotics, isolated<br />
mini-environment front-end solutions, and complete “real time” virtual reality software tools<br />
for the semiconductor and now laboratory aut<strong>omation</strong> markets worldwide. Supporting<br />
OEM sales and manufacturing facilities, Genmark Aut<strong>omation</strong> has support offi ces located<br />
in the United States, Europe, Israel, Japan, Taiwan, Singapore, and Korea.<br />
Booth 810 (10 x 20)<br />
Genomic Solutions develops, manufactures, and sells instrumentation, software, and<br />
consumables used to determine the activity level of genes, that can isolate, identify and<br />
characterize proteins, and to dispense small volumes of biologically important materials.<br />
The company’s products and systems enable researchers to perform complex, high<br />
volume experiments at a lower cost and in less time than traditional techniques. As a result,<br />
Genomic Solutions products and systems facilitate rapid and less expensive drug discovery.<br />
262
263
GenoVision Inc.<br />
901 South Bolmar St., Suite R<br />
West Chester, Pennsylvania 19382<br />
(610) 430-8841; (610) 430-8845 fax<br />
customer.service@genovision.com<br />
www.genovision.com<br />
GenVault Corporation<br />
2101 Faraday Avenue<br />
Carlsbad, California 92008<br />
(760) 268-5200<br />
www.genvault.com<br />
Gilson, Inc.<br />
3000 W. Beltline Highway<br />
Middleton, Wisconsin 53562<br />
(800) 445-7661; (608) 831-4451 fax<br />
sales@gilson.com<br />
www.gilson.com<br />
Glas-Col, LLC<br />
711 Hulman Street<br />
P.O. Box 2128<br />
Terre Haute, Indiana 47802<br />
(812) 235-6167; (812) 234-6975 fax<br />
pinnacle@glascol.com<br />
www.glascol.com<br />
Greiner Bio-One, Inc.<br />
1205 Sarah Street<br />
Longwood, Florida 32750<br />
(407) 333-2800; (407) 333-3001 fax<br />
info@us.gbo.com<br />
www.gbo.com/bioscience<br />
Hamilton Company<br />
4970 Energy Way<br />
Reno, Nevada 89502<br />
(775) 858-3000<br />
www.hamiltoncompany.com<br />
Booth 1535 (10 x 10)<br />
GenoVision is an innovative biotech company. GenoVision has developed a revolutionary<br />
technology for molecular haplotyping, Haplotype Specifi c Extraction, HSE. HSE allows for<br />
the physical separation of naturally diploid DNA into its haploid components for downstream<br />
application. HSE is elegantly simple, and provides a powerful tool for genetic analysis.<br />
Booth 541 (10 x 10)<br />
GenVault provides integrated dynamic archive solutions within an exchange network to<br />
facilitate the distribution of genetic samples and information and enable discovery.<br />
Booth 1025 (20 x 30 Island)<br />
Gilson, Inc. is a manufacturer of high-quality, dependable automated liquid handling<br />
instruments for the pharmaceutical and biotechnology industries. Product lines include<br />
a full range (nano to preparative) of HPLC systems, along with high-throughput robotic<br />
workstations. Gilson also manufactures a line of instruments for OEM customers who<br />
incorporate these instruments into a wide range of existing laboratory systems. Gilson<br />
offers an extensive list of technical training courses that are designed to help customers<br />
maximize the operation of their Gilson instruments and software.<br />
Booth 210 (10 x 10)<br />
Introducing our robotic friendly mixer for liquid handling systems. Operation is by contact<br />
closure or through software control commands. Heating, cooling and concentration of<br />
samples can be incorporated with many systems. Glas-Col mixers are low profi le and can<br />
be designed for your application.<br />
Booth 619 (20 x 20 Island)<br />
Introducing the HTA Array, an innovative 96 well biochip microplate for high-throughput<br />
microarraying. This platform fi ts the standard SBS format and incorporates GREINER’s<br />
expertise with HTS products and current Biochip technology. Also featuring Real-Time<br />
PCR plates, 384 Well Small Volume plates, a full line of Glass Bottom plates, New and<br />
Improved Protein-Coated Labware and an enlarging CrystalStar Family of protein<br />
crystallization plates.<br />
Booth 207 (20 x 30)<br />
Hamilton Company provides a constellation of liquid handling robotics for the<br />
pharmaceutical and biotech industries. The MICROLAB ® STAR and STAR-let use patented<br />
technology for precise pipetting. Hardware options and standard software provide easy<br />
integration with third party devices. The Automated Vacuum System (AVS) allows for<br />
hands-free, feedback-controlled, robotics-friendly 96-well vacuum fi ltration. Visit the<br />
Hamilton booth to see and discuss our multiple fl uid handling technologies, the fl exible<br />
software packages, and the plate-handling robotic solutions.<br />
264
HEMCO Corporation<br />
111 Powell Road<br />
Independence, Missouri 64056<br />
(816) 796-2900; (816) 796-3333 fax<br />
info@hemcocorp.com<br />
www.hemcocorp.com<br />
Hettich<br />
Gartenstraße 100<br />
D-78532 Tuttlingen<br />
Germany<br />
49 7461 705-0; 49 7461 705-125 fax<br />
info@hettichlab.com<br />
www.hettichlab.com<br />
Hudson Control Group, Inc.<br />
10 Stern Avenue<br />
Springfield, New Jersey 07081<br />
(973) 376-7400; (973) 376-8265 fax<br />
info@hudsoncontrol.com<br />
www.hudsoncontrol.com<br />
IDBS Inc.<br />
2 Occam Court<br />
Surrey Research Park<br />
Guildford, Surrey<br />
GU2 7QB<br />
01483 595 000; 01483 595 001 fax<br />
www.id-bs.com<br />
IKO International, Inc.<br />
20170 S. Western Avenue<br />
Torrance, California 90501<br />
(310) 609-3988; (310) 609-3916 fax<br />
wco@ikonet.co.jp<br />
www.ikont.com<br />
Booth 421 (10 x 10)<br />
HEMCO will be exhibiting modular enclosures to isolate lab aut<strong>omation</strong> equipment and<br />
processes. These enclosures can be engineered to exhaust hazardous fumes, maintain a<br />
sterile HEPA fi ltered Class 100 work environment, recirculate precise temperature/humidity<br />
controlled air or supply HEPA fi ltered air in and out for product and personnel protection.<br />
Booth 933 (10 x 10)<br />
HETTICH is one of the leading manufacturers of laboratory centrifuges worldwide. The<br />
Rotanta Robotic centrifuge is the perfect match for system integrators from all fi elds of<br />
automated centrifugation. At LabAut<strong>omation</strong>2004 Hettich will show the machine integrated<br />
in a PVT module designed for use in clinical lab aut<strong>omation</strong>.<br />
Booth 1413 (10 x 20)<br />
Hudson sells robotics for microplate movement capable of integrating with more than 100<br />
devices from other manufacturers. Hudson also provides, through partnerships, liquid<br />
handlers, washers, and dispensers combined with robotics for completely automated<br />
assays as well as automated storage and retrieval systems. The company focuses on the<br />
pharmaceutical and biotechnology markets.<br />
Booth 439 (10 x 10)<br />
IDBS is a leading provider of advanced software solutions for the pharmaceutical,<br />
biotechnology, and agrochemical industries. IDBS’ applications, such as ActivityBase,<br />
are integrated data management, analysis, and decision-making products used in the<br />
drug discovery industry to acquire, manage, and use chemical and biological data across a<br />
spectrum of discovery activities.<br />
Booth 1125 (10 x 10)<br />
Long-Term Maintenance Free Linear Way Series, ML, MH, ME & MUL Series; Maintenance<br />
free for saving-resources. 5 years or 20,000 km lubrication free operation achieved. Anti-<br />
Creep Cage Crossed Roller Way, CRWG Series; Enable for using in vertical axis. Enable<br />
for high-speed and high-acceleration operation. The Smallest All Stainless made Ball Slide<br />
Unit; BWU6 is suitable for Bio-Medical Equipment in Clean room environment. Capilube<br />
Cam Follower, CF…/SG provides maintenance solution. Cleanroom Positioning Table, TC<br />
series achieved ISO Cleanliness Class 1 or less. New Concept Z-axis Elevating Stage; TZ.<br />
Compact XY-Theta linear motor stage; SA65D (65 mm x 65 mm x 56.5 mm) achieved 0.1micron<br />
m resolution.<br />
265<br />
EXHIBITORS
ILS Innovative Labor Systeme<br />
Mittelstrasse 37<br />
D-98714 Stuetzerbach Germany<br />
49(0)36784 50206; 49(0)36784 50207 fax<br />
ils@microsyringes.com<br />
www.microsyringes.com<br />
INA Linear Technik,<br />
A division of INA USA Corp.<br />
308 Springhill Farm Road<br />
Fort Mill, South Carolina 29715<br />
(803) 548-8500; (803) 548-8599 fax<br />
www.ina.com/us<br />
Innovadyne Technologies, Inc.<br />
2835 Duke Court, P.O. Box 7329<br />
Santa Rosa, California 95407-7329<br />
(707) 547-2500; (707) 547-2501 fax<br />
info@innovadyne.com<br />
www.innovadyne.com<br />
Innovative Microplate<br />
1998 Westover Road<br />
Chicopee, Massachusetts 01022<br />
(413) 593-5300; (413) 593-5900 fax<br />
jlipsky@innovativemicroplate.com<br />
www.innovativemicroplate.com<br />
Invetech Instrument<br />
Development and Manufacture<br />
44 Montgomery Street, Suite 1308<br />
San Francisco, California 94104<br />
(415) 533-1483; (415) 693-0826 fax<br />
instruments@invetech.us<br />
www.invetech.us<br />
ISC BioExpress<br />
420 North Kays Drive<br />
Kaysville, Utah 84037<br />
(800) 999-2901; (801) 547-5051 fax<br />
isc@bioexpress.com<br />
www.bioexpress.com<br />
Booth 1229 (10 x 10)<br />
ILS manufactures precision microsyringes from 0,5 µl to 50ml for manual dosing and<br />
automatic systems such as autosamplers, syringe pumps, dilutors and dispensers. For<br />
better precision, maximum chemical resistance and a longer lifetime, all syringes are<br />
made of genuine 3.3 borosilicate glass (DURAN ® = Schott Glaswerke AG). More than 700<br />
types and variations are available from the catalogue. As an OEM supplier ILS assists its<br />
customers in solving their liquid handling and dosing problems. ILS also offers a unique<br />
precision syringe pump VP 9100 for OEM application in microlitre dosage<br />
Booth 1318 (10 x 10)<br />
INA is your one stop source for linear and rotary motion systems—2 and 4 row ball & roller<br />
profi le systems, roundshaft systems, track roller systems, mechanical actuators, roller &<br />
ball screws, ball splines, ball screw support bearings, turntable bearings. State-of-the-art<br />
technology for every application.<br />
Booth 733 (10 x 10)<br />
Innovadyne Technologies, Inc. is a privately held, rapidly growing fl uidics technology<br />
company that manufactures and sells enabling non-contact, low-volume liquid<br />
transfer solutions to end users and leading lab aut<strong>omation</strong> companies. Innovadyne’s<br />
nanopipetting products range from single-channel, OEM-integrated dispensing devices<br />
to self-contained, 96-channel sample transfer instruments.<br />
Booth 814 (10 x 10)<br />
Innovative Microplate offers to take your research beyond the industry standard.<br />
Application specifi c microplates to personalized fi ltration devices. We create solutions that<br />
support your vision and achieve your scientifi c objectives. Complete in-house fabrication<br />
allows us to focus on rapid turnaround and lets you focus on your pursuits.<br />
Booth 435 (10 x 20)<br />
Invetech provides contract instrument design, development and manufacturing services<br />
for leading clinical diagnostics, life science and biotechnology companies. Our track record<br />
includes 20+ automated laboratory and point-of-care instruments since 1988. A 200+<br />
in-house team with systematic ISO 9001, QSR compliant development processes and<br />
FDA registered manufacturing, plus our responsive approach, effectively integrates global<br />
teams to deliver high quality, fast-to-market results at a competitive cost.<br />
Booth 936 (10 x 10)<br />
ISC BioExpress offers wide-ranging technologies and products for the life science<br />
and drug discovery laboratory. Key products include reagents, RNAse/DNAse free<br />
consumables, automated sequencing solutions, and equipment for research procedures<br />
involving HTS, electrophoresis, nucleic acids, hybridization, fi ltration, immunology, and<br />
tissue culture. The extensive equipment offering includes products for gradient thermal<br />
cycling, centrifugation, electroporation, and more.<br />
266
J-Kem Scientific, Inc.<br />
6970 Olive Boulevard<br />
St. Louis, Missouri 63130<br />
(800) 827-4849; (314) 863-6070 fax<br />
jkem911@jkem.com<br />
www.jkem.com<br />
Jencons Scientific, Inc.<br />
800 Bursca Drive, Suite 801<br />
Bridgeville, Pennsylvania 15017<br />
(412) 257-8861; (412) 257-8809 fax<br />
info@jencons.com<br />
www.jenconsusa.com<br />
Jouan Robotics<br />
170 Marcel Drive<br />
Winchester, Virginia 22602<br />
(800) 820-9427<br />
(540) 869-8623; (540) 869-8626 fax<br />
info@jouanroboticsusa.com<br />
www.jouanroboticsusa.com<br />
Julabo USA, Inc.<br />
754 Roble Road<br />
Allentown, Pennsylvania 18109<br />
(800) 458-5226; (610) 231-0260 fax<br />
daniel@julabo.com<br />
www.julabo.com<br />
Jun-Air, USA<br />
1350 Abbott Court<br />
Buffalo Grove, Illinois 60089<br />
(847) 215-9444; (847) 215-9449 fax<br />
tomas.torp@jun-air.com<br />
www.jun-air.com<br />
Kendro Laboratory Products<br />
275 Aiken Road<br />
Asheville, North Carolina 28804<br />
(800) 522-7746<br />
info@kendro.com<br />
www.kendro.com<br />
Booth 1006 (10 x 10)<br />
Offering the Phoenix and our new Eclipse line of custom robotic Workstations for weighing,<br />
dissolution, SPE, reformatting, and custom designs. Eclipse workstations are module<br />
and built-to-order starting at just $13,000. Accessories include homing shakers, fi lter,<br />
SPE, and SPS stations. Parallel synthesis reactors offer built in stirring, inert atmosphere,<br />
and temperature control. Digital temperature controllers regulate any volume or piece of<br />
equipment to 0.1˚ C. Digital vacuum regulator controls pressure and eliminates mercury.<br />
J-KEM makes custom aut<strong>omation</strong> equipment to your specifi cation.<br />
Booth 713 (10 x 10)<br />
Introducing our new range of Millennium microplate products including 96/384 dispensers,<br />
washers and incubator shakers. Also featuring Sealpette & Powerpette manual and<br />
electronic pipetting and dispensing products and BioPlastics Real-Time PCR Tubes and<br />
Plates.<br />
Booth 507 (10 x 30)<br />
Jouan Robotics combines top-level expertise in robotics systems design with the<br />
internationally recognized know-how of Jouan in centrifugation, cold storage, incubation…<br />
Jouan Robotics offers automated modules, such as Automated Centrifuges, Automated<br />
CO2 incubators, and the MolBank, a unique instrument for automated cold storage and<br />
retrieval of microplates. We also develop complete sample preparation and management<br />
workstations based on our products.<br />
Booth 520 (10 x 10)<br />
Julabo is a worldwide manufacturer of constant temperature circulators with a 36-year<br />
reputation for high quality, reliable and durable products, combined with an excellent<br />
support. A wide product range of refrigerated & heating circulators, water baths and<br />
chillers is completed by the highly-dynamic temperature controllers of the Presto- and<br />
Forte HT-series. JULABO—When temperature matters.<br />
Booth 1134 (10 x 10)<br />
Specializing in clean and quiet Air. Powering gas generating equipment, rheometers, and<br />
laboratory instruments. Air bearings, leveling and suspension systems. Medical, dental<br />
and optical equipment and applications. Animatronics, airbrushing, aut<strong>omation</strong>, system<br />
pressurization, and particle analyzing and our newest product, medical grade compressed<br />
air. In-house custom applications and worldwide sales, service, and distribution.<br />
Booth 1524 (10 x 20)<br />
Kendro designs and manufactures the Heraeus brand of Cytomat robot accessible<br />
incubators and storage cha<strong>mbers</strong>. The products come in a wide variety of temperature<br />
ranges (-20 to +70C) and capacities (42 to 924 micro plates) used for cell-based assays,<br />
cell culture, compound storage, ELISA and other biochemical assays. These products are<br />
designed to provide superior environmental control and are easily integrated into most<br />
types and brands of automated<br />
assay systems.<br />
267<br />
EXHIBITORS
Kloehn Company<br />
10000 Banburry Cross Drive<br />
Las Vegas, Nevada 89144<br />
(702) 243-7727 or (800) 358-4342<br />
(702) 243-6036 fax<br />
info@kloehn.com<br />
www.kloehn.com<br />
KMC Systems, Inc.<br />
220 Daniel Webster Highway<br />
Merrimack, New Hampshire 03054<br />
(603) 886-7501; (603) 594-7022 fax<br />
kcushman@kollsman.com<br />
www.kmcsystems.com<br />
LABCON North America<br />
P.O. Box 5559<br />
Petaluma, California 94955<br />
www.labcon.com<br />
Labcyte<br />
1190 Borregas Avenue<br />
Sunnyvale, California 95132<br />
(408) 747-2000; (408) 747-2010 fax<br />
contact@labcyte.com<br />
www.labcyte.com<br />
LabVantage Solutions, Inc.<br />
245 US Highway 22 West<br />
Bridgewater, New Jersey 08807<br />
(908) 707-4100; (908) 707-1179 fax<br />
thermann@labvantage.com<br />
www.labvantage.com<br />
Booth 533 (10 x 10)<br />
Kloehn Company is a primary manufacturer of precision liquid handling components<br />
including syringes, solenoid and shear valves, single and multichannel syringe pumps,<br />
needles and probes, and associated tubing and fi ttings. We specialize in turnkey fl uidic<br />
systems for OEM instrumentation.<br />
Booth 1514 (10 x 10)<br />
Design, development and production of electronic and electromechanical devices,<br />
including diagnostic, therapeutic, and biomedical instrumentation. Expert in all aspects<br />
of engineering, validation, and design for production; 20 +years of medical industry<br />
experience. Flexible turnkey manufacturing with full warranty and depot support. ISO 9001<br />
certifi ed; FDA registered. Full compliance with quality system regulation.<br />
Booth 1129 (10 x 10)<br />
Labcon operates from a 125,000 square foot facility about 45 minutes north of San<br />
Francisco. We develop and market Earth Friendly ® Disposable Pipet Tips for many<br />
automated pipettors. Our focus is on solutions that eliminate or reduce waste from the<br />
disposables you use. We have a variety of refi lling systems for pipet tips available some<br />
of which can be supplied directly to your pipetting station with our proprietary LightsOff<br />
Robots.<br />
Booth 1136 (10 x 30)<br />
Labcyte Inc. provides products to improve effi ciency and decrease costs for liquid<br />
handling in a broad set of life science applications. Labcyte offers the mini-Gene reagent<br />
dispenser for 96, 384 and 1536 well plates, the “mini-Stack” for automated plate handling<br />
and lid removal and the new SII for tabletop pipetting with interchangeable 96/384 channel<br />
pipetting heads and on-board disposable tip changing. For HTS, the BioCross has an<br />
eight-position deck with on-board robot for handling consumables and integrated stackers<br />
for plates, tips or reservoirs and integrates with shakers, vacuum stations, tip washers and<br />
cooling units. Labcyte supplies high-quality disposable tips for the Beckman Biomek ® ,<br />
Multimek ® and FX ® systems and consumables for the Zymark RapidPlate, ® Tecan Genesis, ®<br />
and Perkin/Elmer-Packard Multiprobe ® and Evolution P3 as well as hydrophobic fi lter<br />
barrier tips. Labcyte’s newest product, the Echo 550, uses focused acoustics (ultrasound)<br />
for “touchless” liquid transfer. Having no contact between the device and the solution<br />
being moved means no washing or tips are required. The fi rst system is optimized for the<br />
transfer of 5 to 50 nL of DMSO/water solutions of compound libraries with CV’s of less<br />
than 8% for dispensing into empty or fi lled plates.<br />
Booth 1221 (10 x 10)<br />
LabVantage is a leader in Enterprise LIMS and Life Sciences LIMS solutions, and an<br />
Advanced IBM Business Partner. LabVantage provides intelligent sample and experiment<br />
data management solutions for traditional manufacturing QA/QC, pharmaceutical QC,<br />
genomics, proteomics, and high throughput screening. LabVantage also offers services<br />
such as industry and technology consulting, implementation services, application training,<br />
and extensive customer support services. Since 1981, LabVantage has implemented<br />
hundreds of laboratory information systems across a broad range of industries.<br />
268
Lathrop Engineering, Inc.<br />
1101 S. Winchester Boulevard, B110<br />
San Jose, California 95128<br />
(408) 260-2111; (408) 260-2242 fax<br />
bobl@lathropengineering.com<br />
www.lathropengineering.com<br />
Lawrence Berkeley National<br />
Laboratory<br />
1 Cyclotron Road<br />
Berkeley, California 94720<br />
(510) 486-4000<br />
www.lbl.gov<br />
LEAP Technologies<br />
P.O. Box 969<br />
Carrboro, North Carolina 27510<br />
(800) 229-8814<br />
info@leaptec.com<br />
www.leaptec.com<br />
Liconic Instruments<br />
Industriestrasse 170<br />
9493 Mauren<br />
Principality of Liechtenstein<br />
423 373 63 39; 423 373 53 59 fax<br />
www.liconic.com<br />
Los Alamos National Laboratory<br />
P.O.Box 1663<br />
Los Alamos, New Mexico 87545<br />
(505) 665-9091<br />
www.lanl.gov<br />
Booth 1425 (10 x 10)<br />
Lathrop is a fast track, full service product development organization. From concept to<br />
pre-production prototypes and manufacturing transfer, we have expertise in microfl uidics,<br />
optics, robotics, thermal, electronics, industrial design, packaging, embedded software,<br />
plastics, FEA analysis, systems integration, design for manufacturability, serviceability and<br />
cost reduction. Exceeding customer’s expectations by design since 1982. ISO9001:2000.<br />
Booth 941 (10 x 10)<br />
Lawrence Berkeley National Laboratory is a major multi-program national laboratory,<br />
managed by the University of California for the Department of Energy (DOE). Berkeley<br />
Lab’s research produces transferable innovations and inventions. A variety of mechanisms<br />
are available through licensing and research partnership programs that help assist<br />
with commercialization from Berkeley Lab to the industry. Following a 72 year tradition<br />
of scientifi c inquiry and discovery, Berkeley Lab has developed technologies with a<br />
wide range of applications across industry, especially in the area of energy effi ciency,<br />
biotechnology, medical imaging and instrumentation, nanotechnologies, advance<br />
materials, environmental protection, and semiconductor processing. For a more detailed<br />
list of technologies available for licensing and collaborative research, please visit our web<br />
site at www.lbl.gov/tt/.<br />
Booth 1036 (10 x 10)<br />
LEAP Technologies will feature our 2D iD Gel Processing System for better gel imaging and<br />
spot cutting in proteomics. LEAP specializes in front-end aut<strong>omation</strong> for mass spec, LC,<br />
GC, and SPE. LEAP Shell is a new, unique, sample centric software product for the Mass<br />
Spec Lab. It synchronizes peripheral devices of the MS, including pumps, autosamplers,<br />
valves, and heaters.<br />
Booth 206 (10 x 20)<br />
Liconic AG is the world’s leading manufacturer of automated incubators for life science<br />
research. In addition, Liconic manufactures automated pick-and-place systems for<br />
innovative components, including those used in future-orientated communication<br />
technologies.<br />
Booth 824 (10 x 10)<br />
Los Alamos National Laboratory (LANL) has a large and diverse bioscience research and<br />
technology development program funded in excess of $150M per year. The program<br />
features hundreds of academic and corporate collaborations and a fast-growing<br />
intellectual property portfolio. LANL is actively interested in developing additional<br />
collaborations with researchers in the life sciences industry. LANL will showcase some of<br />
its latest research as well as identify collaboration mechanisms, partnering opportunities,<br />
as well as technology transfer and<br />
licensing opportunities.<br />
269<br />
EXHIBITORS
270
Luminex Corporation<br />
12212 Technology Boulevard<br />
Austin, Texas 78727<br />
(512) 219-8020; (512) 219-5195 fax<br />
info@luminexcorp.com<br />
www.luminexcorp.com<br />
Macherey-Nagel<br />
6 S. Third Street, Suite 402<br />
Easton, Pennsylvania 18042<br />
(610) 559-9848; (610) 559-9878 fax<br />
sales-us@mn-net.com<br />
www.mn-net.com<br />
MATECH<br />
31304 Via Colinas, Suite 102<br />
Westlake Village, California 91362<br />
www.matech.us<br />
MatriCal, Inc.<br />
665 N. Riverpoint Boulevard<br />
Spokane, Washington 99208<br />
(509) 343-6225; (509) 343-9224 fax<br />
www.matrical.com<br />
Matrix Technologies Corporation<br />
22 Friars Drive<br />
Hudson, New Hampshire 03051<br />
(603) 595-0505; (603) 595-0106 fax<br />
www.matrixtechcorp.com<br />
Booth 735 (10 x 20)<br />
Luminex Corporation develops, manufactures and markets proprietary biological testing<br />
technologies with applications throughout the life sciences industry. The company’s<br />
xMAP system is an open-architecture, multi-analyte technology platform that delivers<br />
fast, accurate and cost-effective bioassay results to markets as diverse as pharmaceutical<br />
drug discovery, clinical diagnostics and biomedical research, including the genomics and<br />
proteomics research markets. The company’s xMAP technology is sold worldwide and is<br />
already in use in leading research laboratories as well as major pharmaceutical, diagnostic,<br />
and biotechnology companies.<br />
Booth 709 (10 x 10)<br />
Macherey-Nagel is a world-leader in the development, production, and distribution of<br />
DNA/RNA purifi cation kits based on fi lter, silica, magnetic bead, or alternative materials.<br />
The products are suitable for the isolation of plasmid DNA, PCR products, total RNA and<br />
genomic DNA on all common laboratory automated workstations. Please also inquire<br />
about our clinical and molecular diagnostic, plant, and forensic kits.<br />
Booth 934 (10 x 10)<br />
Since its founding in 1989, MATECH has become recognized as a world class research<br />
and development laboratory in the areas of optical, electronic, bio-materials, and high<br />
temperature ceramic materials by chemical polymerization methods. MATECH was<br />
founded by Dr. Edward J. A. Pope. During the past 13 years, MATECH has generated<br />
in excess of $5.5 million in revenues from private sector clients and product sales.<br />
In addition, MATECH manufactures and sells Fluorescent Reference Standards<br />
(www.matech.us) for calibrating plate-reading machines used in medical analysis, drug<br />
discovery, high throughput screening, and clinical diagnostics. MATECH’s past and<br />
present clients include ATK Thiokol, Pratt & Whitney Engine Division, Boeing IDS, Goodrich<br />
Aerospace, Westinghouse Nuclear Division, HITCO, Refractory Composites, Inc., Ceramic<br />
Composites Innovations (CCI), NALCO Chemical Corporation, Roche Diagnostics (formerly<br />
Boehringer-Mannheim Corporation), Beckman-Coulter (formerly Beckman Instruments),<br />
Inc., Schott Gas Burner Division, and Careside Diagnostics. This is only a partial listing.<br />
Booth 1328 (10 x 10)<br />
MatriCal is a leading developer of instrumentation for the life science research market.<br />
The company’s innovative products include a patented Microwell plate line featuring<br />
high quality low volume plates for HTS. The MatriStore, MatriSeal, MatriPress compound<br />
storage systems and 2D MicroBank minitube cassettes provide a robust and fl exible<br />
solution for compound management from freezers to fully integrated solutions. The<br />
SonicMan HT sonicator enables redissolution of compounds and extraction of target<br />
proteins, RNA/DNA, and enzymes in 96, 384, and 1536-well plates. The MatriCycler<br />
offers a patented robotic compatible solution for 1-20uL reactions for ultra high speed<br />
amplication in 384 and 1536-well formats.<br />
Booth 1506 (10 x 40)<br />
Matrix Technologies Corporation, a leader in liquid handling technology, offers an extensive<br />
line of devices and consumables. The company’s liquid handling instrumentation ranges<br />
from hand held pipettors to automated workstations. In addition, Matrix offers a wide<br />
range of high quality pipette tips. Also available are bar-coded liquid storage systems,<br />
proprietary microarray disposables and a broad selection of microwell assay plates.<br />
271<br />
EXHIBITORS
MDL Information Systems<br />
14600 Catalina Street<br />
San Leandro, California 94577<br />
(510) 895-1313; (510) 614-3608 fax<br />
l.hill@mdl.com<br />
www.mdl.com<br />
MéCour Temperature Control<br />
10 Merrimack River Road<br />
Groveland, Massachusetts 01834<br />
(877) 398-6085<br />
mail@mecour.com<br />
www.mecour.com<br />
Merlin BioProducts BV<br />
Charles Petitweg 37-11<br />
4827 HJ Breda<br />
The Netherlands<br />
31 076-5816660, 31 076-5815560 fax<br />
info@merlin-bioproducts.com<br />
www.merlin-bioproducts.com<br />
Mettler-Toledo Autochem<br />
562 Bunker Court<br />
Vernon Hills, Illinois 60061<br />
www.mtautochem.com<br />
Micralyne, Inc.<br />
1911-94 Street<br />
Edmonton, Alberta T6N 1E6 Canada<br />
(780) 431-4403; (780) 431-4422 fax<br />
vwalker@micralyne.com<br />
www.micralyne.com<br />
Booth 1112 (10 x 10)<br />
MDL ® will demonstrate integrated biology and chemistry experiment management<br />
solutions from plate management through data analysis and reporting. The new workfl oworiented<br />
MDL ® Plate Manager with open architecture greatly simplifi es the creation and<br />
management of plate and sample information. MDL ® Assay Explorer makes it easy to<br />
capture, analyze, visualize, and store biological data. See how to import data from Excel<br />
worksheets, save images and documents as results, and interact with new 3D visualization<br />
and statistics tools.<br />
Booth 1120 (10 x 10)<br />
MéCour’s circulator-driven Thermal Blocks offer precise (+/- 0.1º C) temperature<br />
distribution over the entire Block; sealed unit to eliminate hazards or contamination;<br />
operate hot or cold between +/- 100º C. Perfect for laboratory manufacturing and<br />
automated robotic systems. Available in standard or custom confi gurations per customer’s<br />
specifi c requirements. All Thermal Blocks easily connect to the circulator and come with a<br />
lifetime warranty.<br />
Booth 1600 (10 x 10)<br />
Merlin BioProducts BV is proud to present the ViAll reader. ViAll, a unique system,<br />
based on 2 high resolution CCD cameras and newly developed software, reads all known<br />
2D tubes and racks within 1 second. The unique ViAll software makes a distinction<br />
between “no tube” and “illegible tube”, after which each tube can be inspected and<br />
corrected using the zoom option. The generated data can be exported to fi le. The ViAll<br />
software can also interface with an Aut<strong>omation</strong> ® server application. ViAll software can<br />
be integrated into ActiveX ® compatible software. The ViAll reader and software allows<br />
you to mix several brands (Micronic/Matrix/Abgene) and types of tubes in one rack. The<br />
orientation of a rack is automatically detected and corrected, thus eliminating wrongly<br />
orientated racks. This feature will reduce the number of human errors, thus enabling a<br />
higher throughput. ViAll software is also available in combination with a ViAll scanner.<br />
Both products are available for OEM.<br />
Booth 535 (10 x 20)<br />
Mettler-Toledo AutoChem provides solutions that enable scientists to dramatically<br />
reduce the time to discover and develop new compounds. These solutions automate<br />
the discovery process of: reagent preparation (USP), synthesis (MiniBlock/XT), work-up<br />
(ALLEXis), purifi cation (PrepSFC, MultiGram and MiniGram) and analysis (Analytical SFC,<br />
SFC/MS and MiniGram), compound management (Balance and Label Automators and<br />
FlexiWeigh).<br />
Booth 1032 (10 x 10)<br />
Micralyne is a pioneer and leader in the development and OEM manufacturing of<br />
microfabricated and MEMS-based components. MEMS products enable orders of<br />
magnitude improvements in biomedical instrumentation. Micralyne’s MEMS solutions<br />
include lab-on-a-chip devices and microfl uidic devices for drug discovery and drug<br />
delivery. Micralyne is a profi table and growing MEMS company.<br />
272
MicroGroup<br />
7 Industrial Park Road<br />
Medway, Massachusetts 02053<br />
(800) 255-8823; (508) 533-9881 fax<br />
sburke@microgroup.com<br />
www.microgroup.com<br />
Micronic Systems<br />
PMB 301, 4017 Washington Road<br />
McMurray, Pennsylvania 15317<br />
(724) 941-6411; (724) 941-8662 fax<br />
mortek@aol.com<br />
www.micronic.com<br />
Micropump, Inc.<br />
1402 NE 136th Avenue<br />
Vancouver, Washington 98684<br />
www.micropump.com<br />
Microscan Systems, Inc.<br />
1201 SW 7th Street<br />
Renton, Washington 98055<br />
(425) 226-5700; (425) 226-8250 fax<br />
info@microscan.com<br />
www.microscan.com<br />
Millipore Corporation<br />
290 Concord Road<br />
Billerica, Massachusetts 01821<br />
(800) 645-5476; (800) 645-5439 fax<br />
www.millipore.com<br />
MJ Research, Inc.<br />
590 Lincoln Street<br />
Waltham, Massachusetts 02451<br />
(888) 735-8437; (617) 923-8080 fax<br />
sales@mjr.com<br />
www.mjr.com<br />
Booth 906 (10 x 10)<br />
MicroGroup is a vertically integrated contract manufacturer focused on supplying<br />
stainless steel tubing, components and assemblies. 7,000,000 feet of stainless steel<br />
and nickel based alloy tubing under 1 inch in diameter alloy for short lead times.<br />
Premium ID fi nish tubing is available for probe applications.<br />
Booth 441 (10 x 10)<br />
Micronic offers a comprehensive line of sample storage tubes (2D coded, alpha numeric<br />
and non coded) racks, caps, bar code readers, and automated capping systems. We will<br />
be exhibiting the following products:<br />
• Tracker 2D coded tubes<br />
• Traxis 2D coded tubes<br />
• New 0.5 ml 2D coded tubes with minimal dead volume<br />
• New Color coded caps: 8 colors available<br />
• New Compact High Through Put Bar Code Reader: reads 96 tubes in 1 second<br />
• New Split Cap<br />
• New Starter Pack System: consists of a Table Model scanner, software, 72 racks of<br />
2D coded tubes, cap clusters, cap mat sealer—all for only $4,400.00<br />
• New Automated Capper and Decappers Systems.<br />
Booth 519 (10 x 20)<br />
Micropump ® offers an extensive line of precision low fl ow external and micro annular<br />
gear, piston, peristaltic, centrifugal, and vane pumps, and programmable drives from<br />
Ismatec. ® For complete fl uidic assemblies, Micropump can package its products with high<br />
precision switching valves from Rheodyne ® and vacuum pumps and compressors from<br />
Gast. Through its added product lines and increased fl exibility in custom OEM design<br />
capabilities, Micropump can provide fl uid handling system components and assemblies for<br />
a variety of applications.<br />
Booth 1427 (10 x 10)<br />
Microscan is a leading manufacturer of high-speed, fi xed-position bar code scanners and<br />
CCD readers capable of reading linear and 2D codes. As the only manufacturer focused<br />
exclusively on fi xed-position bar-code scanning technology, Microscan’s products and<br />
support are unmatched. Microscan scanners can be easily mounted along conveyor lines<br />
or integrated into other equipment. With a suite of customizable products and a full-service<br />
applications lab, Microscan can meet the demands of nearly every application.<br />
Booth 907 (10 x 10)<br />
Millipore will be introducing its new HTS platform for high throughput screening of<br />
ADME and Pre-ADME applications. On display will be Millipore’s comprehensive line of<br />
aut<strong>omation</strong> compatible assay systems for solubility, cell-based (Caco and MDCK), and<br />
non-cell based (PAMPA and permeability) drug absorption assays and bound vs. free drug.<br />
Aut<strong>omation</strong> compatible products and protocols for proteomics and genomics applications<br />
are also available.<br />
Booth 1014 (10 x 10)<br />
Exhibited will be MJ’s full line of Peltier thermal cyclers, including the DNA Engine,<br />
Tetrad, Dyad, PTC-100 ® and Minicycler ® lines—and the novel DNA Engine Opticon ®<br />
2 fl uorescence system for real-time analysis. Also displayed will be innovative reagent<br />
systems and vessels for thermal cycling.<br />
273<br />
EXHIBITORS
Modern Drug Discovery/<br />
Chemical & Engineering News<br />
Centcom Ltd<br />
19035 Old Detroit Road, Suite 203<br />
Rocky River, Ohio 44116<br />
(440) 331-5151; (440) 331-3432 fax<br />
poorman@acs.org<br />
Molecular BioProducts<br />
9880 Mesa Rim Road<br />
San Diego, California 92121<br />
www.mbpinc.com<br />
Molecular Devices Corporation<br />
1311 Orleans Drive<br />
Sunnyvale, California 94089<br />
(408) 747-1700; (408) 747-3601 fax<br />
info@moldev.com<br />
www.moldev.com<br />
Motoman, Inc.<br />
805 Liberty Lane<br />
West Carrollton, Ohio 45449<br />
(937) 847-6200; (937) 847-6277 fax<br />
info@motoman.com<br />
www.motoman.com<br />
Multi-Contact USA<br />
5560 Skylane Boulevard<br />
Santa Rosa, California 95403<br />
(707) 575-7575; (707) 575-7373 fax<br />
mailbox@multi-contact-usa.com<br />
www.multi-contact-usa.com<br />
®<br />
Booth 109 (10 x 10)<br />
MODERN DRUG DISCOVERY…covering the world of genomics, proteomics, economics<br />
and clinical trials, MDD focuses on serving the growing needs of scientists involved in drug<br />
discovery and life science research. This practical, how-to-publication includes articles<br />
that focus on new drugs, new drug targets and technologies, economic and regulatory<br />
concerns, plus instructive and inviting features on how drug discovery and development<br />
actually takes place.<br />
Booth 1007 (10 x 20)<br />
Molecular BioProducts is a plastics injection molding company whose core focus is to<br />
provide value added liquid handling solutions to life science researchers in the Biotech,<br />
Academic and Pharmaceutical workplace. MBP is the leading provider of disposable lab<br />
supplies to the Life Science Industry around the globe.<br />
Booth 719 (20 x 20 Island)<br />
Molecular Devices Corporation is a leading developer of high-performance, bioanalytical<br />
measurement systems that accelerate and improve drug discovery and other life sciences<br />
research. The company’s systems enable pharmaceutical and biotechnology companies<br />
to leverage advances in genomics and combinatorial chemistry by facilitating the high<br />
throughput and cost effective identifi cation and evaluation of drug candidates. The<br />
company’s instrument systems are based on its advanced core technologies which<br />
integrate its expertise in engineering, molecular and cell biology and chemistry and are<br />
fundamental tools for drug discovery and life sciences research.<br />
Booth 1212 (10 x 20)<br />
Motoman’s RobotWorld* RW05 system is the basis for a new, high-throughput micro-plate<br />
ELISA processing system. The demo cell features integrated process equipment, including<br />
washer, dispenser, and plate reader, as well as a plate hotel/incubator. Motoman’s new<br />
AutoSorter*II features a high-speed SCARA robot equipped with Motoman’s integrated bar<br />
code reader and innovative tube gripper for sorting in a fl exible environment. It provides<br />
high throughput (approximately 1,000 tubes per hour), instrument-specifi c rack loading,<br />
and bar code label orientation.<br />
Booth 1238 (10 x 10)<br />
From design to manufacturing, Multi-Contact connectors and test accessories are<br />
produced to satisfy the highest industry standards. MC’s state-of-the-art connector<br />
technology offers both standard and custom designed solutions for a wide and diverse<br />
spectrum of applications.<br />
Medical Applications: 1.5mm and 2mm “Touchproof” safety sockets, plugs, leads and<br />
adapters. POAG (equipotential grounding) pins and sockets are manufactured to DIN and<br />
IEC recommendations. 3-in-1 Universal electrode clips for direct connections to various<br />
types of electrodes.<br />
274
MWG Biotech, Inc.<br />
4170 Mendenhall Oaks Parkway<br />
Suite 160<br />
High Point, North Carolina 27265<br />
(336) 812-9995; (877) 694-2832 toll free<br />
(336) 812-9983 fax<br />
www.THE-MWG.com<br />
Nanostream<br />
580 Sierra Madre Villa Avenue<br />
Pasadena, California 91107<br />
(626) 351-8200; (626) 351-8201 fax<br />
info@nanostream.com<br />
www.nanostream.com<br />
National Instruments<br />
11500 N. Mopac<br />
Austin, Texas 78759-3504<br />
(512) 683-5723; (512) 683-5775 fax<br />
marti.mccollough@ni.com<br />
www.ni.com<br />
NB Corp of America<br />
2157 O’Toole Avenue, Suite D<br />
San Jose, California 95131<br />
(888) 562-4175; (408) 435-1850 fax<br />
www.nbcorporation.com<br />
New England Small Tube<br />
Corporation<br />
480 Charles Bancroft Highway<br />
Litchfield, New Hampshire 03052<br />
(603) 429-1600; (603) 429-1601 fax<br />
erica@nesmalltube.com<br />
www.nesmalltube.com<br />
Booth 137 (10 x 20)<br />
MWG Biotech is a global genomics solution provider offering a variety of products and<br />
services for research needs from genome to gene function. Through our four business<br />
units – Genomic Technology, Genomic Diagnosis, Genomic Information and Genomic<br />
Synthesis we offer full-service solutions for thermal cyclers and robotic instrumentation<br />
for lab aut<strong>omation</strong>, microarrays and oligo sets, custom sequencing, and custom<br />
oligonucleotide synthesis.<br />
Booth 232 (10 x 10)<br />
Nanostream, Inc. (Pasadena, Calif.) provides high throughput microfl uidic analytical<br />
systems to companies involved in drug discovery and development. Nanostream’s<br />
systems are based on proprietary, modular product design that allows for timely<br />
introduction of new products according to market needs. Nanostream is pioneering micro<br />
parallel liquid chromatography (µPLC) with its premier product, the Veloce system. The<br />
Veloce system, designed for scientists who can benefi t from greater sample analysis<br />
capacity, integrates with existing workfl ow and increases the throughput of established<br />
HPLC (high performance liquid chromatography) techniques. Nanostream is a registered<br />
trademark of Nanostream, Inc. The stylized “N” logo, “Brio,” “Veloce” and “Snap-n-Flow”<br />
are trademarks of Nanostream, Inc.<br />
Booth 1511 (10 x 10)<br />
The diversity of the life science fi eld is mirrored in the wide range of applications solved<br />
by National Instruments products. From biotechnology to medical device testing, from<br />
analytical instrumentation to physiological monitoring, NI products provide highly fl exible and<br />
cost effective solutions. For the scientist, these tools help complete your research on time.<br />
For the engineer, these tools cut test time on your next application. National Instruments<br />
partners offer a wide range of services and solutions based on National Instruments<br />
products. Also, look for National Instruments Alliance Member Data Science Aut<strong>omation</strong> in<br />
our booth. Booth 1511, one stop shopping for all of your lab aut<strong>omation</strong> needs!<br />
Booth 1035 (10 x 10)<br />
NB liner products: from slide bush, fl ange type, Topball, and conventional types; slide<br />
units; shafting; slide ways; slide guides; ball spline; BG actuator. Offering wide range in size<br />
and material options to meet the most demanding of linear applications around the world.<br />
Most inch and metric dimensional standards are available for immediate delivery from local<br />
inventories. Custom machining of case hardened shaft also available. New Products: RV<br />
gonio Way, RBW plastic unit, SYBS miniature slide way.<br />
Booth 1329 (10 x 10)<br />
New England Small Tube is an ISO 9001: 2000 Certifi ed company, providing small<br />
diameter tube bending and fabrication. Our capabilities include burr-free cutting, bending,<br />
brazing, swaging, fl aring, bulging, micropolishing, electropolishing, passivation and Tefl on<br />
coating. New England Small Tube is a specialist in custom fabrication of sampling and<br />
reagent probes, preheater tubes, dispensing manifolds, and 96/384 well-plate tips!<br />
275<br />
EXHIBITORS
276
NSK Precision America, Inc.<br />
2171 Executive Drive, Suite 100<br />
Addison, Illinois 60101<br />
(630) 620-8500<br />
www.nskprecision.com<br />
NuGenesis Technologies<br />
1900 West Park Drive<br />
Westborough, Massachusetts 01581<br />
(508) 616-4578; (508) 616-9880 fax<br />
info@nugenesis.com<br />
www.nugenesis.com<br />
NUNC Brand Products<br />
Nalge Nunc International<br />
75 Panorama Creek Drive<br />
Rochester, New York 14625<br />
(585) 586-8800<br />
www.nuncbrand.com<br />
Opticon, Inc.<br />
8 Olympic Drive<br />
Orangeburg, New York 10962<br />
(845) 365-0090; (845) 365-1251 fax<br />
www.opticonusa.com<br />
webmaster@opticonusa.com<br />
Oriental Motor USA Corp.<br />
2570 W. 237th Street<br />
Torrance, California 90505<br />
(800) 816-6867; (800) 309-7999 fax<br />
sales@orientalmotor.com<br />
www.orientalmotor.com<br />
Booth 1011 (10 x 20)<br />
NSK Precision America is an ISO certifi ed manufacturer of precision motion control<br />
components and systems. NSK’s product line includes, but is not limited to; precision<br />
ground and rolled ball screws, precision linear ball bearing guides, and direct drive rotary<br />
and linear motors. NSK is the world’s leading manufacturer of precision ground and rolled<br />
ball screws. NSK’s precision linear ball bearing guides are offered in standard and stainless<br />
steel, in a wide range of styles for many applications. NSK’s line of Direct Drive rotary and<br />
linear motors produce high torque at low speed while yielding fast accelerations, shorter<br />
cycle times, zero backlash, precise positioning, and lower friction. U.S. Manufacturing.<br />
Booth 715 (10 x 10)<br />
The NuGenesis Scientifi c Data Management System (SDMS) web-based platform provides<br />
a foundation for scientifi c data preservation and for integrating the collaborative fl ow<br />
of information through existing LIMS, EDMS, e-lab notebooks, visualization/analytics<br />
programs, and Microsoft Offi ce applications. Scientists fi nd, review and share their<br />
critical laboratory data, making key decisions and reducing cycle time. NuGenesis SDMS<br />
integrates with existing IT infrastructure, ensures data security, offers central or distribution<br />
administration and is quickly deployed with minimal IT resources. Over 300 life science<br />
companies use NuGenesis SDMS for long-term data preservation, intellectual property<br />
protection and 21CFR11 compliance. Custom integration and validation services are<br />
available.<br />
Booth 532 (10 x 10)<br />
NUNC offers a comprehensive line of cutting-edge products for HTS & aut<strong>omation</strong>. New<br />
microarray products include ArrayCote 16-well slides and 96-well plates, polymer slides,<br />
glass slides & accessories. Shallow Well 384 and 1536 plates optimize throughput, and<br />
Low Profi le BioAssay Dishes are designed for automated colony screening. 96-well Filter<br />
Plates isolate high quality Amplifi cation products and plasmid DNA. The white 384-well<br />
Low Crosstalk plate is optimized for luminescence and scintillation proximity assays.<br />
Booth 939 (10 x 10)<br />
More than 20 years of proven performance has made Opticon the choice for bar code<br />
readers. Select from a complete family of laser, CCD and 2D imagers, offering choices<br />
of size, scan speed and reading distance. Work closely with our engineers as they help<br />
integrate the right reader into your design. We modify to meet your needs. Unquestioned<br />
quality and durability. We are so sure of our product, Opticon now offers a 7 year warranty<br />
on fi xed mount CCD readers. Custom built—built to last!<br />
Booth 1612 (10 x 10)<br />
Oriental Motor USA is a manufacturer of motion control products. 5-Phase and 2-Phase<br />
stepping motors, motors only or as a complete system with driver and controller. AC<br />
motors, gearmotors, and DC brushless motors with controllers. AC/DC cooling products –<br />
fans, blowers, and cross fl ow fans.<br />
277<br />
EXHIBITORS
Orochem Technologies, Inc.<br />
762 Burr Oak Drive<br />
Westmont, Illinois 60559<br />
(630) 887-0616; (630) 887-0753 fax<br />
sales@orochem.com<br />
www.orochem.com<br />
Oyster Bay Pump Works, Inc..<br />
PO Box 725, 78 Midland Avenue<br />
Hicksville, New York 11802<br />
(516) 933-4500; (516) 933-4501 fax<br />
info@obpw.com<br />
www.obpw.com<br />
Pall Life Sciences<br />
600 S. Wagner Road<br />
Ann Arbor, Michigan 48103<br />
(734) 665-0651; (734) 913-6114 fax<br />
lab@pall.com<br />
www.pall.com/lab<br />
Parker Hannifin Corporation<br />
6035 Parkland Boulevard<br />
Cleveland, Ohio 44124<br />
(216) 896-3000<br />
www.parker.com<br />
PerkinElmer Life and Analytical<br />
Sciences<br />
710 Bridgeport Avenue<br />
Shelton, Connecticut 06484<br />
(800) 762-4000; (203) 944-4904 fax<br />
info@perkinelmer.com<br />
www.perkinelmer.com<br />
Booth 1041 (10 x 10)<br />
Orochem offers a total process solution for bioanalytical, combinatorial chemistry, and<br />
drug discovery laboratories.<br />
• One of the largest selections of solid phase extraction products in high throughput<br />
96-well extraction plates as well as in traditional column and cartridge formats.<br />
• 96-well plates, 384-well plates functionalized with high performance “Orpheus” and<br />
“Celerity- Polymeric” sorbents, unique membranes and Coatings<br />
• Orpheus sorbents with minimal silanol content, custom packed in a format<br />
of your choice.<br />
• Vacuum Manifold, Vacuum Controller, Sample Concentrator, Robotic Liquid<br />
Handler, Reactor Block<br />
• Newly added Injection Molding capabilities!<br />
Booth 1428 (10 x 10)<br />
Internationally recognized specialist in microplate processing aut<strong>omation</strong> and precision<br />
liquid dispensing. OBPW automates complete processes incorporating precise liquid<br />
dispensing (nanoliters to liters), washing, blocking, drying, printing, labeling, hot stamping,<br />
lidding, sealing, incubation, fi ll verifi cation, data acquisition & more. We automate<br />
processing of 96, 384, and 1536-well plates, test tubes, slides, membranes, and more.<br />
Most applications concern medical diagnostic test kit manufacturing and laboratory<br />
aut<strong>omation</strong> for drug discovery.<br />
Booth 1411 (10 x 10)<br />
Pall Life Sciences, a leader in fi ltration, purifi cation, separation and detection technologies,<br />
offers a wide range of products for genomics, proteomics, drug discovery and HTS<br />
applications. Our products provide maximum recoveries, ease of use and lot to lot<br />
consistency. Products highlighted include 96 and 384 well fi lter plates, microarray slides,<br />
centrifugal devices, and blotting membranes.<br />
Booth 636 (10 x 30)<br />
Parker aut<strong>omation</strong> provides motion control solutions ideal for Life Science applications;<br />
i.e., liquid handling, micro-arraying, scanning and storage and retrieval robots. Parker<br />
motion technology includes programmable stepper/servo motors, PCI bus motion<br />
controller cards, miniature linear motor positioners and sealed positioning tables designed<br />
for 24/7 operation.<br />
Booth 625 (20 x 30 Island)<br />
PerkinElmer is a global technology leader providing products and services to customers in<br />
health sciences and other advanced technology markets that require innovation, precision<br />
and reliability. Operating through three business units—Life and Analytical Sciences,<br />
Optoelectronics, and Fluid Sciences—PerkinElmer, Inc. provides scientifi c instruments,<br />
consumables and services to the pharmaceutical, biomedical, environmental testing<br />
and industrial markets. PerkinElmer has a broad global sales and service network, with<br />
approximately 2,500 representatives operating in 45 countries, and marketing its products<br />
in 125 countries.<br />
278
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279
PharmaGenomics Magazine<br />
485 Route 1 South, Building. F, Suite 100<br />
Iselin, New Jersey 08830<br />
(732) 596-0276; (732) 596-0048 fax<br />
www.pharmagenomicsonline.com<br />
Pierce Biotechnology, Inc.<br />
P.O. Box 117<br />
Rockford, Illinois 61105<br />
(815) 968-0747; (815) 968-7316 fax<br />
cs@piercenet.com<br />
www.piercenet.com<br />
Pneutronics Division of Parker<br />
Hannifin Corporation<br />
26 Clinton Drive, Unit 103<br />
Hollis, New Hampshire 03049<br />
(603) 595-1500; (603) 595-8080 fax<br />
ljoseph@parker.com<br />
www.pneutronics.com<br />
Point Technologies, Inc.<br />
6859 N. Foothills Highway<br />
Boulder, Colorado 80302<br />
www.pointtech.com<br />
Booth 841 (10 x 10)<br />
Written by scientists for scientists, PharmaGenomics delivers peer-reviewed, applicationsoriented,<br />
technical feature articles to 40,015 BPA audited industry subscribers.*<br />
PharmaGenomics is the global authority in life science and drug discovery research.<br />
Discover how PharmaGenomics will increase your infl uence and expand your reach.<br />
Booth 1218 (10 x 10)<br />
Pierce Biotechnology manufactures products for Drug Discovery including homogeneous<br />
detection assay systems, multiplex assays and a variety of pre-blocked coated<br />
microplates. The Pierce IQ Assay Platform provides high-throughput analysis of kinases<br />
and phosphatases. IQ Assays are universal and do not require the use of antibodies.<br />
The assay is directly scalable to 384- and 1536-well formats. Custom IQ Assays are<br />
available. Searchlight Arrays quantify up to 16 proteins per well in two hours using<br />
5–50 µl of sample. Assay offerings include measurement of cytokines, chemokines,<br />
angiogenic factors, MMPs and kinases. Searchlight Assays offer the accuracy and<br />
simplicity of a traditional sandwich ELISA in a multiplexed format with excellent sensitivity.<br />
Custom array design and testing services are available. Coated microplates include<br />
NeutrAvidin Biotin-Binding Protein and Streptavidin (in regular and high-binding capacity<br />
formats); Biotin; HisGrab Metal Chelate; Glutathione; anti-GST; anti-GFP; dextrin;<br />
activated maleimide; activated maleic anhydride; Protein A, G and L; and CellScreen<br />
Poly-D-Lysine; Poly-L-Lysine; and Collagen I. Custom plate-coating chemistries<br />
and chemistries on coated slides, beads and membranes are available through the<br />
ChoiceCoat ® Custom Plate-Coating Service.<br />
Booth 739 (10 x 20)<br />
Pneutronics Division of Parker Hannifi n is a leader in miniature solenoid and miniature<br />
proportional valve technologies, miniature fl uidic systems integration and design<br />
solutions while bringing value through an organizational focus on lean enterprise<br />
initiatives, continuous improvement, innovative product development, application specifi c<br />
designs, and premier customer service. For more information on Pneutronics, go to<br />
www.pneutronics.com. With annual sales exceeding $6.5 billion, Parker Hannifi n is the<br />
world’s leading diversifi ed manufacturer of fl uidic control, fi ltration, motion, and control<br />
technologies and systems.<br />
Booth 1409 (10 x 10)<br />
Point Technologies, Inc. is the world’s fastest growing microarray spotting pin<br />
manufacturer. The company uses precision electrochemical etching and electrical<br />
discharge machining (EDM) to manufacture genomic and proteomic microarray spotting<br />
pins for all arrayers. It is one of the few companies in the world that can point and<br />
electropolish tungsten wire. The company has recently announced ACCELERATOR<br />
Brand microarray spotting pin products for the worldwide gene expression market. The<br />
new pins have the following characteristics, which have been widely hailed by customers:<br />
• Material: Tungsten<br />
Benefi t: Long life due to increased hardness (4X that of SS) and vastly improved<br />
corrosion resistance compared to existing stainless steel pins<br />
• Color-coded Collars to denote tip and slit size<br />
• Sequentially numbered collars for traceability from microarray lab back to<br />
manufacturing<br />
• New Packaging to facilitate handling and storage<br />
• Radiused tips (Patent Pending) for higher density, consistent spots<br />
• Conical, Electropolished, Tapered tips for decreased substrate damage and improved<br />
spot morphology.<br />
280
Popper & Sons, Inc.<br />
300 Denton Avenue<br />
New Hyde Park, New York 11040<br />
(516) 248-0300; (516) 747-1188 fax<br />
sales@popperandsons.com<br />
www.popperandsons.com<br />
Porvair Sciences Ltd.<br />
6 Shepperton Business Park<br />
Shepperton TW17 8BA United Kingdom<br />
44 1932 240255; 44 1932 254393 fax<br />
int.sales@porvair.com<br />
www.porvair-sciences.com<br />
Process Analysis and Aut<strong>omation</strong><br />
Falcon House, Fernhill Road, Farnborough<br />
Hampshire, GU14 9RX<br />
United Kingdom<br />
01252 373000; 01252 371922 fax<br />
info@paa.co.uk<br />
www.paa.co.uk<br />
Promega Corporation<br />
2800 Woods Hollow Road<br />
Madison, Wisconsin 53711<br />
www.promega.com<br />
Protedyne Corporation<br />
1000 Day Hill Road<br />
Windsor, Connecticut 06095<br />
(860) 683-1860; (860) 683-4178 fax<br />
pattid@protedyne.com<br />
www.protedyne.com<br />
Booth 419 (10 x 10)<br />
Popper & Sons, Inc. is an ISO 9001:2000 US Manufacturer of needles, probes and tips<br />
for laboratory aut<strong>omation</strong>. We offer standard, custom and OEM solutions for sample<br />
preparation, liquid handling, genomic aut<strong>omation</strong>, HTS, pipetting and micro-dispensing.<br />
Products include: blunt tubes, non-coring needles, OD/ID Tefl on, Ceramic and PSX<br />
ceramic coating.<br />
Booth 1518 (10 x 10)<br />
Part of the Porvair plc group of companies, Porvair Sciences specializes in the production<br />
of Microplates for assays, drug screening, sample storage and sample preparation. In<br />
addition to the wide range of Microplates, the company offers instruments for sample<br />
concentration and microplate sealing. New products at the booth will be the MiniVap<br />
sample concentrator and the Thermobond Auto, a plate heat sealing instrument for<br />
automated or manual use.<br />
Booth 1533 (10 x 10)<br />
Process Analysis & Aut<strong>omation</strong> Ltd offer fl exible laboratory aut<strong>omation</strong> software solutions.<br />
OVERLORD Workstation is a real-time scheduler that can be used to control simple<br />
to complex automated workcells where real-time decisions are required. OVERLORD<br />
Scheduler is a pre-emptive scheduler with the ability to control assays to tight time<br />
tolerances, with the ability to run multiple assays simultaneously and also insert extra<br />
assays during a run. Both packages can be distributed over a number of PCs.<br />
Booth 1320 (10 x 10)<br />
Promega Corporation is a worldwide leader in developing and implementing reagent<br />
systems to provide automated solution packages to our customers. Promega’s proven<br />
expertise in working with instrument manufacturers to generate total solutions for<br />
customers offers reagent and throughput fl exibility that is unmatched. Our product<br />
implementation portfolio includes: nucleic acid isolation and analysis; cellular analysis;<br />
proteomics; bioluminescence and reporter assays and Genetic Identity.<br />
Booth 525 (20 x 30 Island)<br />
Protedyne’s unique approach combines scientifi c and aut<strong>omation</strong> expertise with industrialquality<br />
aut<strong>omation</strong> and sophisticated data management. The foundation of the Company<br />
is the BioCube System platform, which brings advanced industrial-aut<strong>omation</strong> capabilities<br />
into the life science laboratory in a fast, fl exible, and reliable package. The company also<br />
provides contract laboratory research services utilizing the BioCube System. Protedyne is<br />
headquartered in Windsor, Connecticut, and has offi ces in Martinsried (Munich), Germany.<br />
For more information call (860) 683-1860 in North America or +(49) 89-8565-3400 in<br />
Europe, or visit Protedyne on the web at www.protedyne.com.<br />
281<br />
EXHIBITORS
RAPP POLYMERE GmbH<br />
Ernst Simon Str. 9<br />
D 72072 Tuebingen<br />
49 7071 763 157; 49 7071 763 158 fax<br />
rapp-polymere@t-online.de<br />
www.rapp-polymere.com<br />
REMP AG<br />
Weststrasse 12<br />
Oberdiessbach 3672 Switzerland<br />
41 31 770 70 70; 41 31 770 72 66 fax<br />
info@remp.com<br />
www.remp.com<br />
ReTiSoft, Inc.<br />
48 Forty-First Street, Suite 1<br />
Toronto, Ontario M8W 3N6<br />
Canada<br />
(416) 521-9720; (416) 521-9277 fax<br />
prodziew@retisoft.ca<br />
www.retisoft.ca<br />
Rheodyne LLC<br />
P.O. Box 1909<br />
Rohnert Park, California 94927-1909<br />
(707) 588-2000; (707) 588-2020 fax<br />
sales@rheodyne.com<br />
www.rheodyne.com<br />
Richard Scientific, Inc./<br />
Sepiatec GmbH<br />
285 Bel Marin Keys Boulevard<br />
Novato, California, 94949(<br />
(415) 883-2888; (415) 382-1922 fax<br />
info@richardscientifi c.com<br />
www.richardscientifi c.com<br />
Rigaku<br />
9009 New Trails Drive<br />
The Woodlands, Texas 77381<br />
(281) 363-1033; (281) 364-3628 fax<br />
info@rigakumsc.com<br />
www.rigakumsc.com<br />
Booth 1606 (10 x 10)<br />
Since over one decade now, RAPP POLYMERE is one of the leading companies and<br />
fi rst address for polymer supports. RAPP POLYMERE offers a broad range of novel<br />
resins for solid phase chemistry combinatorial chemistry, library generation, peptide and<br />
oligonucleotide synthesis, as well as for diagnostic applications. Our products and services<br />
are: Resins for solid phase organic synthesis, custom synthesis, peptide synthesis,<br />
functionalized polymers, chemistry development, synthesizing modules, miniaturization,<br />
HPLC columns.<br />
Booth 913 (10 x 20)<br />
REMP specializes in solutions for life science Compound and Materials Management<br />
organizations around the world. Our products include systems, workstations, consumables<br />
and software applications for compound storage and retrieval, compound cherry-picking,<br />
climate control, plate replication and reformatting, powder dosing, vial weighing, tube<br />
capping and uncapping, thermal sealing, and piercing. Our corporate offi ce is located in<br />
Switzerland with subsidiary offi ces located in Germany, Japan and the United States.<br />
Booth 334 (10 x 10)<br />
ReTiSoft products include a software framework that simplifi es instrument integration, a<br />
scheduling software, 3D simulation viewer, and instrument testers for the laboratory<br />
aut<strong>omation</strong> market.<br />
To allow companies to wisely choose their laboratory instruments we implemented<br />
an open system for the integration, controlling and monitoring of diverse laboratory<br />
instruments. With our software your lab aut<strong>omation</strong> system can quickly become a mix of<br />
instruments from multiple hardware vendors. You can competitively select the best-ofbreed<br />
equipment to meet your unique lab aut<strong>omation</strong> requirements.<br />
Booth 521 (10 x 10)<br />
Rheodyne will exhibit TitanEX—a family of long-life, economical, low-pressure, fl uid<br />
valves for OEMs. TitanEX integrates several new technologies for a dramatic enhancement<br />
in shear-face valve performance. Advanced composite materials qualify TitanEX<br />
performance to 6,000,000 actuations. A unique, (patent pending) fi ttingless connection<br />
system simplifi es tubing interface to a fi nger tight operation. TitanEX—for stream selection,<br />
sample injection, and fl uid switching applications.<br />
Booth 234 (10 x 10)<br />
Innovative solutions for HPLC, SPE and LC/MS: The sepmatix concept in parallel HPLC<br />
offers an ultimate solution when high sample throughput is needed. Eight chromatographic<br />
channels are run using one single pump. Sepmatix modules for parallel injection, fl ow<br />
control, multiplex detection and parallel fraction collection are available as stand-alone<br />
units. The automated online HPLC/SPE (1D/2D) coupling technology of the sepboxR<br />
systems provide total separation of complex mixtures of natural products or combinatorial<br />
compounds.<br />
Booth 536 (10 x 10)<br />
Rigaku/MSC, is the world’s leading resource for single-crystal X-ray diffraction<br />
hardware, software and contract services. Rigaku/MSC offers fully integrated small<br />
and macromolecule applications, Rigaku generators, X-ray optical systems, cryocooling<br />
devices and peripheral devices. In addition, Rigaku/MSC provides small and<br />
macromolecular structure determination services on a contract basis.<br />
282
Rixan Associates<br />
7560 Paragon Road<br />
Dayton, Ohio 45459<br />
(937) 438-3005; (937) 438-0130 fax<br />
robots@rixan.com<br />
www.rixan.com<br />
RoboDesign International, Inc.<br />
5920 Pasteur Court<br />
Carlsbad, California 92008<br />
(760) 438-5282; (760) 438-5286 fax<br />
info@robodesign.com<br />
www.robodesign.com<br />
Roche Applied Science<br />
9115 Hague Road, Building B<br />
Indianapolis, Indiana 46250<br />
(800) 428-5433; (317) 521-7317 fax<br />
www.roche-applied-science.com<br />
Roche Instrument Center Ltd.<br />
Forrenstrasse<br />
Rotkreuz 6343 Switzerland<br />
41 41 799 2555; 41 41 799 2287 fax<br />
oem.instrumentation@roche.com<br />
www.roche-oem-instrumentation.com<br />
RSF Electronics, Inc.<br />
2880 Gold Tailings Ct.<br />
Rancho Cordova, California 95670<br />
(916) 852-6660; (916) 852-6664 fax<br />
sales@rsf.net<br />
www.rsf.net<br />
RTS Life Science International<br />
Northbank, Irlam<br />
Manchester M44 5AY, United Kingdom<br />
44 161 777 2000; 44 161 777 2002 fax<br />
lifescience.info@rts-group.com<br />
www.rtslifescience.com<br />
Booth 1401 (20 x 20)<br />
Rixan Associates, Inc. is the North American Distributor of Mitsubishi robots and Samsung<br />
robots. Rixan provides robots, turn-key aut<strong>omation</strong> systems, training, service, engineering,<br />
linear tracks and custom end-of-arm tooling.<br />
Booth 538 (10 x 20)<br />
RoboDesign International, Inc. is a premier source for the development, design,<br />
manufacture, and support of custom and standard aut<strong>omation</strong> solutions and<br />
instrumentation for the life sciences industry. With our broad range of technical expertise,<br />
we are uniquely positioned to provide a wide array of engineering services, from rapid<br />
prototyping to OEM design and manufacturing. RoboDesign has developed products for<br />
the genomics, proteomics, hospital pharmacy, and laboratory aut<strong>omation</strong> industries. Our<br />
applications include protein crystal inspection and analysis, microarraying, and low volume<br />
liquid dispensing. Whatever your needs, RoboDesign is your single engineering source.<br />
Booth 524 (10 x 20)<br />
Roche Applied Science supplies reagents and instrumentation for life-science research.<br />
Our innovative instruments include the LightCycler Instrument for quantitative PCR and<br />
mutation detection; the MagNA Pure LC Instrument for fully automated nucleic-acid<br />
isolation from a wide variety of sample types; and the New LightTyper Instrument for<br />
simplifi ed post-PCR SNP genotyping.<br />
Booth 1429 (10 x 10)<br />
The Roche Instrument Center in Rotkreuz, Switzerland, a member of the Roche group,<br />
is a leading supplier of state-of-the-art diagnostic and analytical instruments. Since<br />
1969, over 35,000 instruments have been manufactured and installed worldwide. As one<br />
of the few unique OEM providers mastering a seamless OEM process in one location,<br />
we turn customer needs into comprehensive product solutions in the fi eld of laboratory<br />
aut<strong>omation</strong>. Now available is a new Poly-Pipettor 96/384, a compact and high-precision<br />
OEM module fi tting easily into various liquid handling systems.<br />
Booth 1601 (10 x 10)<br />
RSF Electronics manufactures and distributes linear encoders, digital readouts, digital<br />
depth probes and associated electronics for precision motion control (closed loop<br />
feedback) and measurement applications. Linear resolutions from 10 microns (0.0005")<br />
to 10 nanometers (0.0000005"). Non-contacting designs and fully enclosed designs have<br />
large air gaps and mounting tolerances. Models are available for high-speeds and include<br />
integrated limit switches. Vacuum rated, harsh environment and self-guided encoders are<br />
also available. Additional information can be found at www.rsf.net<br />
Booth 1119 (10 x 20)<br />
A leading supplier of automated laboratory systems, RTS Life Science provides industrial<br />
quality robotic systems for drug discovery applications including advanced sample storage<br />
and retrieval products, HTS systems—Assay Platform, fully automated cell culture—<br />
acCellerator, High Throughput Structural Biology and MDI /DPI testing. Customized to<br />
individual client requirements, systems utilize proprietary instrumentation and sophisticated<br />
SPRINT scheduling software to produce the optimum integrated solution.<br />
283<br />
EXHIBITORS
SAGE Publications<br />
2455 Teller Road<br />
Thousand Oaks, CA 91320<br />
(800) 818-7243; (805) 499-0871 fax<br />
lisa_lamont@sagepub.com<br />
www.sagepub.com<br />
SANYO Scientific<br />
SANYO Sales & Supply Co.<br />
1062 Thorndale Avenue<br />
Bensenville, Illinois 60106<br />
(800) 858-8442; (630) 238-0074 fax<br />
info@sanyobiomedical.com<br />
www.sanyobiomedical.com<br />
SCIENCE/AAAS<br />
1200 New York Avenue<br />
Washington, DC 20005<br />
(202) 326-6417; (202) 842-1065 fax<br />
www.scienceonline.org<br />
Scientific Specialties, Inc.<br />
1310 Thurman Street<br />
Lodi, California 95240<br />
(209) 333-2120; (209) 333-8623 fax<br />
info@ssi-plastics.com<br />
www.ssi-plastics.com<br />
Scinomix, Inc.<br />
4046 Wedgeway Court<br />
Earth City, Michigan 63045<br />
(314) 298-9800<br />
www.scinomix.com<br />
Scivex<br />
619 W. Oak Street<br />
Oak Harbor, Washington 98277<br />
www.scivex.com<br />
Booth 239 (10 x 10)<br />
SAGE Publications-an independent international publisher in the social sciences,<br />
technology and medicine-provides journals, books, and electronic media of the highest<br />
caliber. Researchers, students, and professionals have relied on our innovative resources<br />
for over 35 years. Please stop by our booth or visit us at www.sagepub.com to review our<br />
publications.<br />
Booth 806 (10 x 10)<br />
SANYO Biomedical, a division of SANYO Electric Co. of Osaka, Japan is a leading,<br />
vertically integrated, manufacturer and worldwide distributor of ultra low temperature<br />
freezers, incubators, laboratory refrigeration, environmentally controlled equipment, as<br />
well as HTS Aut<strong>omation</strong> Equipment. SANYO utilizes unique design and component<br />
technologies to deliver reliable and consistent test and storage conditions. Our web site is:<br />
www.sanyobiomedical.com.<br />
Booth 832 (10 x 10)<br />
Founded in 1880 by Thomas Edison, Science ranks as the world’s leading scientifi c<br />
journal. Each week, Science provides nearly 140,000 subscribers around the world with<br />
peer-reviewed original research, scientifi c research articles, and reports, science and<br />
research news as well as policy forums and perspectives on current topics. Scientists<br />
can also access the journal online at www.scienceonline.org. The site includes a<br />
comprehensive recruitment site, www.sciencecareers.org, offering job listings, career<br />
advice and a resume/cv database as well as a database of scientifi c meetings at<br />
www.sciencemeetings.org.<br />
Booth 935 (10 x 10)<br />
Scientifi c Specialties Inc., based in Lodi, California, is a manufacturer of injection-molded<br />
plastic consumables for use in life science research applications.<br />
Within the diverse product range, SSI specializes in tips for robotic workstations,<br />
manufactured to provide an exceptionally high level of quality assurance to meet the<br />
highest expectations.<br />
SSI’s easily recognizable precision products are available worldwide via an established<br />
network of dealers and distributors. ALA 2003 sees the launch of several new products<br />
suitable for HTS and other automated situations.<br />
Booth 1106 (10 x 20)<br />
Scinomix provides the scientifi c community with fl exible, modular aut<strong>omation</strong> and data<br />
management systems for use in research and drug discovery.<br />
Booth 1419 (10 x 20)<br />
Scivex leads the industry in creating and developing high-value components and<br />
technologies for Fluidic Instrumentation—through the world-class expertise of its<br />
component divisions: Upchurch Scientifi c, Sapphire Engineering and JL White Technical<br />
Sales. Scivex sets the standard in both quality designs and innovative modules for OEMs<br />
serving the Analytical Chemistry, Biotechnology and Clinical Diagnostic disciplines.<br />
Product lines include: tubing, precision dispense pumps and pump components, micro-<br />
and nanoscale components, valves, fi ttings, fi lters/frits, fl ow cells, and seals.<br />
284
Seyonic SA<br />
Puits-Godet 12<br />
Neuchatel 2000, Switzerland<br />
41 32 729 28 28; 41 32 729 28 29 fax<br />
marc.boillat@seyonic.com<br />
www.seyonic.com<br />
SGE, Inc.<br />
2007 Kramer Lane<br />
Austin, Texas 78758<br />
(800) 945-6154; (512) 836-9159 fax<br />
usa@sge.com<br />
www.sge.com<br />
Shimadzu Biotech<br />
7102 Riverwood Drive<br />
Columbia, Maryland 21046<br />
(888) 744-1611<br />
www.shimadzu.com<br />
Sias<br />
Churchmans Center<br />
11A Parkway Circle<br />
New Castle, Delaware 19720<br />
(302) 326-0433; (302) 326-0492<br />
info@sias.biz<br />
www.sias.biz<br />
Silicon Valley Scientific, Inc.<br />
4059 Clipper Court<br />
Fremont, California 94538<br />
(510) 438-9300; (510) 438-9311 fax<br />
sales@svsci.com<br />
www.svsci.com<br />
Booth 1127 (10 x 10)<br />
Seyonic SA is a company specialized in the application of microsystem technology for<br />
measurement and control in laboratory instrumentation. One of our core competencies<br />
is accurate small volume liquid handling in the sub-microliter range. Seyonic assists in<br />
the development of applications and also takes care of component manufacturing and<br />
specialized assembly and testing of OEM instrument sub-systems.<br />
Booth 1015 (10 x 10)<br />
Analytical and Life Science Instrument Consumables—ProteCol capillary HPLC columns<br />
and accessories, micro volume syringes, capillary GC columns, injectors, ferrules, fi ttings<br />
(including SilTiteä ferrules for GC use), septa, micro valves, fi lters, inlet liners, tubing,<br />
HPLC columns; GC/MS Supplies—ETP electron multipliers, jet separators, column change<br />
system; Instruments—pyrolyzer, multidimensional system.<br />
Booth 1139 (10 x 10)<br />
Shimadzu Biotech is a new strategic global business unit of Shimadzu Corporation,<br />
focused on the biotechnology and pharmaceutical sectors. It has been created to bring<br />
together a strong solutions-based offering to accelerate the progress of biotechnology<br />
research and development. Shimadzu Biotech offers a wide range of key products,<br />
covering technologies from DNA sequencing to high performance mass spectrometry, to<br />
provide an integrated approach to the fast growing proteomics and genomics markets. It<br />
also supports other applications, including micro-satellite DNA and SNP analysis for drug<br />
discovery.<br />
Booth 236 (10 x 20)<br />
Sias develops and supplies innovative multi-tipped robotic liquid handling systems. Xantus<br />
is a modular robotic pipetting platform, combining fl exible liquid handling with robotic<br />
manipulation. With all functionality, including the pumps, housed in the arm, Xantus design<br />
simplifi es both system integration and stand alone robotic aut<strong>omation</strong>. Integrating modules<br />
such as shakers, heaters, vacuum modules and the Ixion robot friendly microplate<br />
centrifuge can expand Xantus functionality.<br />
Booth 115 (10 x 10)<br />
Silicon Valley Scientifi c provides lab aut<strong>omation</strong> services, instrument and software design<br />
and development, microfl uidic and microchip development, and contract services in the<br />
areas of surface chemistry and DNA sequencing. Solutions include the LabRAT rapid<br />
aut<strong>omation</strong> toolkit software, surface chemistry analysis systems, and custom development<br />
service packages. We can provide integrated solutions including laboratory robotic<br />
hardware, automated instruments, and automated protocols for a variety of laboratory<br />
needs.<br />
285<br />
EXHIBITORS
Silex Microsystems AB<br />
Box 595<br />
Bruttovägen 1<br />
SE-175 26 Järfälla<br />
Sweden<br />
46 8 580 249 13; 46 8 580 249 01<br />
helene.andersson@silex.se<br />
www.silexmicrosystems.com<br />
SKF USA Inc.<br />
1530 Valley Center Parkway<br />
Bethlehem, Pensylvania 18017<br />
( 800) 541-3624; (610) 861-4811 fax<br />
tarek.bugaighis@skf.com<br />
www.skf.com<br />
SLR Systems, Inc.<br />
23112 NE 22nd Street<br />
Camas, Washington 98607<br />
(360) 833-8500; (360) 833-8600 fax<br />
rodney@slrsystems.com<br />
www.slrsystems.com<br />
SMAC<br />
5807 Van Allen Way<br />
Carlsbad, California 92008<br />
(760) 929-8170; (760) 929-7588 fax<br />
www.smac-mca.com<br />
Sorenson BioScience<br />
6507 S. 400 W.<br />
Salt Lake City, Utah 84107<br />
(801) 266-9334; (801) 262-0433 fax<br />
sbiinfo@sorbio.com<br />
www.sorbio.com<br />
Booth 237 (10 x 10)<br />
Silex Microsystems AB mission is to help telecommunication and life science companies<br />
pioneer new applications using MEMS technology and stay a step ahead. We use MEMS<br />
to design and make microsystems, like sensors, actuators, and other precision structures<br />
to be integrated with customers‚ end products. Every component is designed and<br />
produced in our own production fab and we guarantee that everything is of highest quality.<br />
Booth 141 (10 x 10)<br />
SKF the worlds largest manufacturer of bearings will exhibit its array of radial and linear<br />
motion solutions for laboratory aut<strong>omation</strong>. Products on display will include radial<br />
bearings, precision rails with anti-creep solutions, miniature profi le rails in stainless steel,<br />
actuators, ball screws and ball screw support bearings. In addition, SKF also offers a wide<br />
array of linear ball bearings, precision slides, and linear drive systems.<br />
Booth 133 (10 x 10)<br />
SLR Systems is a third-party laboratory aut<strong>omation</strong> integrator. As such, we design and<br />
manufacture custom laboratory aut<strong>omation</strong> and light industrial robotic systems, as well as<br />
laboratory aut<strong>omation</strong> peripherals such as automated heating blocks for our clients. We<br />
are not affi liated with any robot manufacturer or other lab aut<strong>omation</strong> provider; therefore<br />
we provide can you with the best robotic or dedicated lab aut<strong>omation</strong> platform on which to<br />
base your laboratory aut<strong>omation</strong> application. In other words, we work for you to solve your<br />
problems. Not for a robot manufacture to sell a robot. In fact, many of our systems are<br />
dedicated aut<strong>omation</strong> that do not even utilize a robot in the conventional sense.<br />
Booth 1539 (10 x 10)<br />
SMAC manufactures a variety of unique single pole linear motor based servo actuators for<br />
precision aut<strong>omation</strong>. These devices are capable of providing programmable positioning<br />
to sub micro level, programmable velocity, and uniquely, programmable forces. These<br />
capabilities allow SMAC Moving Coil actuators to “do work and verify its accuracy at the<br />
same time.”<br />
Booth 336 (10 x 10)<br />
Sorenson BioScience manufactures a wide range of liquid handling consumable products<br />
for use with laboratory aut<strong>omation</strong>. From robotic tips to plates, Sorenson sets the standard<br />
for quality. Automated QC instruments inspect tips for straightness and insure plates are<br />
free of pinholes. Low Binding Polymer Technology reduces binding of DNA and proteins<br />
to tips. All products certifi ed free of RNase/DNase, human DNA, PCR inhibitors, and<br />
pyrogens.<br />
286
287
Spark Holland, Inc.<br />
666 Plainsboro Road, Suite 1336<br />
Plainsboro, New Jersey 08536<br />
(609) 799-7250; (609) 799-8250 fax<br />
usa@sparkholland.com<br />
www.sparkholland.com<br />
Sparton Medical Solutions<br />
5612 Johnson Lake Road<br />
DeLeon Springs, Florida 32130<br />
(386) 985-4631; (386) 985-5036 fax<br />
rkundinger@sparton.com<br />
www.sparton.com/service/medical/<br />
medical.htm<br />
SPEX CertiPrep<br />
203 Norcross Avenue<br />
Metuchen, New Jersey 08840<br />
(800) 522-7739; (732) 603-9647 fax<br />
sampleprep@spexcsp.com<br />
www.spexcsp.com<br />
Stäubli Corporation<br />
201 Parkway West<br />
Duncan South Carolina 29334<br />
(864) 486-5416<br />
Stäubli Corporation – West Coast<br />
6773-D Sierra Court<br />
Dublin, California 94568<br />
(925) 551-7090<br />
www.staublirobotics.com<br />
Booth 620 (10 x 10)<br />
Spark is a world class provider of innovative sample introduction, extraction and separation<br />
technology. As recognized OEM partner, we offer a complete line of autosamplers including<br />
Spark’s new High Throughput Conditioned Autosampler Reliance. Our new online-SPE<br />
front end system Symbiosis offers a unique automated solution for LC-MS. As a total<br />
solution, it provides true aut<strong>omation</strong> of sample prep, better sensitivity and allows you to run<br />
your existing methods as well, without any hardware changes.<br />
Booth 618 (10 x 10)<br />
Sparton is an engineering and manufacturing services provider with over 100 years of<br />
successful history in the electronics industry. Sparton specializes in the development<br />
and implementation of custom solutions for electromechanical systems and devices.<br />
Applications include robotics, laboratory instrumentation, microprocessor based systems,<br />
and devices for the surgical, therapeutic, and diagnostic medical markets. Locations<br />
throughout North America.<br />
Booth 1325 (10 x 10)<br />
SPEX CertiPrep will display two unique products. The Geno/Grinder is a new grinding/<br />
pulverizing mill used to prepare plant tissue for nucleic acid, yeast, enzyme, and protein<br />
extractions. The Freezer/Mill is a cryogenic grinder capable of producing a fi ne powder<br />
from samples such as plant/animal tissue; plastic, rubber and paper; pharmaceuticals, and<br />
heat-sensitive materials.<br />
Booth 1240 (10 x 10)<br />
Staubli Corporation – Robotics Division offers affordable fully articulated robots specifi cally<br />
designed for lab aut<strong>omation</strong>. Our RX and TX line of ultra clean, high speed robots will<br />
enable you to increase process integrity, traceability and throughput. The Staubli robots<br />
are already working in a diverse range of applications in assay plate handling, vaccine<br />
cell culture handling, syringe tray handling, drug discovery and batch handling. The line<br />
of Staubli robots will easily fi t into your layout with their spherical work envelope and easy<br />
to program PC based controller. Please stop by to visit us to learn more of our laboratory<br />
dedicated robot—the “TX” series.”<br />
288
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289
Tecan/Tecan Systems, Inc.<br />
4022 Stirrup Creek Drive Suite 310<br />
Durham, North Carolina 27703<br />
(919) 361-5200; (919) 361-5201 fax<br />
info@tecan.com<br />
2450 Zanker Road<br />
San Jose, California 95131<br />
(800) 231-0711; (408) 953-3101 fax<br />
tecansystemsinfo@tecan.com<br />
www.tecan.com<br />
Technical Manufacturing<br />
Corporation<br />
15 Centennial Drive<br />
Peabody, Massachusetts 01960<br />
(800) 542-9725; (978) 531-8682 fax<br />
sales@techmfg.com<br />
www.techmfg.com<br />
TekCel, Inc.<br />
103 South Street<br />
Hopkinton, Massachusetts 01748<br />
(508) 544-7000; (508) 544-7001 fax<br />
info@tekcel.com<br />
www.tekcel.com<br />
TEKnova<br />
355 Princeton Avenue<br />
Half Moon Bay, California 94019<br />
www.teknova.com<br />
TeleChem Int/ArrayIt.com<br />
524 E. Weddell Drive, Suite 3<br />
Sunnyvale, California 94089<br />
(408) 744-1331; (408) 744-1711 fax<br />
www.arrayit.com<br />
Booth 407 (20 x 50 Island)<br />
Tecan is a leading player in the fast growing Life Sciences supply industry that specializes<br />
in the development, production, and distribution of enabling solutions for the discovery<br />
of pharmaceutical substances, as well as for genomics, proteomics, and diagnostics.<br />
With a long history in the liquid handling and microplate processing business, Tecan is<br />
synonymous with outstanding performance, effi ciency and superior technical support and<br />
service. Tecan offers a wide selection of liquid handling platforms, microplate readers/<br />
washers and microarray instrumentation to address many of the profound challenges<br />
facing biomedical laboratories today. Tecan clients are leading pharmaceutical and<br />
biotechnology companies, university research departments and diagnostic laboratories.<br />
Tecan Systems, Inc., formerly known as Cavro Scientifi c Instruments, Inc., is the world’s<br />
leading OEM supplier of automated liquid handling products for manufacturers of<br />
laboratory instrumentation. Products include highly precise syringe pumps, complete robot<br />
systems for laboratory aut<strong>omation</strong> and application development software. Tecan Systems<br />
also offers customized engineering and manufacturing solutions under an ISO 9001<br />
certifi ed and QSR compliant quality system.<br />
Booth 1114 (10 x 10)<br />
TMC’s CleanTop ® Steel Honeycomb Optical Breadboards are an ideal base for mounting<br />
optical sub-assemblies and robotic aut<strong>omation</strong> equipment. The patented technology<br />
provides a fl at, stiff, damped, stainless steel mounting surface with custom sizes, shapes,<br />
and hole patterns to meet a wide range of applications. TMC also manufactures precision<br />
fl oor vibration isolation systems. New products include STACIS ® piezoelectric active<br />
vibration isolators, Lightweight Breadboards, Cleanroom Compatible systems and Radius<br />
Corner Tops. TMC has full custom capabilities.<br />
Booth 1101 (20 x 20 Island)<br />
TekCel, Inc. is a leading innovator of laboratory aut<strong>omation</strong> for Life Science research.<br />
TekCel’s solutions yield dramatic improvements in effi ciency and effectiveness by<br />
automating manual processes, integrating applications and maximizing process controls<br />
to ensure biological and chemical sample integrity and resulting data fi delity. TekCel’s<br />
modular, scalable, environmentally controlled family of products allow you to invest in<br />
what you need today and seamlessly scale up to meet your future growth. Solutions<br />
include sample management aut<strong>omation</strong>—Plate Management System, Tube Management<br />
System; liquid handling platforms—TekBench, TekBench-SP; software and proprietary<br />
consumables addressing researchers’ needs from archive compound storage through<br />
screening result.<br />
Booth 518 (10 x 10)<br />
TEKnova is a molecular biology based manufacturing company that offers a complete<br />
line of plated media, solutions and reagents that support the standard molecular biology<br />
procedures given in current protocols in molecular cloning, molecular biology, and high<br />
through-put applications. TEKnova has opened a new 30,000 sq. foot state of the art<br />
facility to better serve all your media needs. We are ready to automate your lab media<br />
requirements.<br />
Booth 840 (10 x 10)<br />
TeleChem/ArrayIt.com produces a superior quality collection of Genome and Protein<br />
mining tools, kits and reagents, priced for research. “SpotBot” the personal automated<br />
microarrayer, holds 14 substrates. It utilizes TeleChem’s patented miniature stealth<br />
microspotting technology the same printing technology relied upon by 18 micorarray<br />
robotic manufacturers world wide. “SpotBot” is priced under $14,000. We manufacture<br />
ultra sensitive custom Microarrays, in a cleanroom, using our patented Micorarray printing<br />
pin technology on proprietary superfl at glass substrates.<br />
290
The Aut<strong>omation</strong> Partnership<br />
3411 Silverside Road, Webster Building<br />
Wilmington, Delaware 19810<br />
(302) 478-9060; (302) 478-9575 fax<br />
info@aut<strong>omation</strong>partnership.com<br />
www.aut<strong>omation</strong>partnership.com<br />
The Lee Company<br />
2 Pettipaug Road, P.O. Box 424<br />
Westbrook, Connecticut 06498-0424<br />
(860) 399-6281; (860) 399-2270 fax<br />
inquiry@theleeco.com<br />
www.theleeco.com<br />
Thermo Electron<br />
5344 John Lucas Drive<br />
Burlington, Ontario L7L 6A6<br />
Canada<br />
(905) 332-2000 x319; (905) 332-1114 fax<br />
christy.jacobs@thermo.com<br />
www.thermo.com<br />
THK America<br />
200 East Commerce Drive<br />
Schaumburg, Illinois 60173<br />
www.thk.com<br />
Titertek Instruments<br />
330 Wynn Drive<br />
Huntsville, Alabama 35805<br />
(256) 859-8600; (256) 859-8698 fax<br />
inquiry@titertek.com<br />
www.titertek.com<br />
Titian Software Ltd.<br />
100 Borough High Street<br />
London, SE1 1LB United Kingdom<br />
44 20 7863 3060; 44 20 7863 3001 fax<br />
info@titian.co.uk<br />
www.titian.co.uk<br />
Booth 1224 (10 x 20)<br />
The Aut<strong>omation</strong> Partnership is a world leader in the design and development of advanced<br />
industrial aut<strong>omation</strong> solutions for the life science industry. With a successful track<br />
record in automating and integrating processes for cell culture, compound and sample<br />
management, liquid handling and ultra high throughput screening, the company continues<br />
to develop and implement market driven aut<strong>omation</strong> solutions to meet the discovery,<br />
development and commercial challenges of the 21st Century.<br />
Booth 1225 (10 x 10)<br />
The Lee Company manufactures a complete line of precision, miniature fl uid control<br />
products offering reliable, consistent performance. On display are 2 and 3-way inert and<br />
non-inert solenoid valves, high speed micro-dispensing valves, fi xed and variable volume<br />
dispense pumps and associated inert fl uid handling components. Featured are new microdispensing<br />
valves capable of high speed nanoliter dispensing, new calibrated orifi ces<br />
designed for installation in plastics, and a new integrated pump/valve dispensing system.<br />
Booth 507 (20 x 50 Island)<br />
Thermo Electron Corporation is a world leader in high-tech instruments. The Thermo CRS<br />
Dimension4 aut<strong>omation</strong> platform and scalable CRS POLARA software offer a powerful<br />
combination of simplicity, reliability, fl exibility, and ultra-high throughput. The extensive<br />
range of Thermo microplate-based products (photometers, fl uorometers, luminometers,<br />
reagent dispensers, washers and microplates) offers trusted quality with consistent results.<br />
One example for effi cient use of such tools is the Thermo KingFisher instrument for protein<br />
and nucleic acid purifi cation. Thermo Electron Corporation is a leading solution provider for<br />
pharma research, drug discovery and biotechnology research.<br />
Booth 212 (10 x 20)<br />
The world leader in linear motion technology, THK originated recalculating ball bearing<br />
carriages on a square shape rail. By implementing these Linear Guides with a patented<br />
cage ball technology system, THK brings dramatic improvements in life, smoothness,<br />
noise, maintenance and overall cost. Our components consist of precision linear guides,<br />
ball screws, cross roller rings, link balls, rod ends and actuators. Available in stainless steel<br />
and miniature models for all types of lab aut<strong>omation</strong> and instrumentation equipment and<br />
biotechnology companies. ISO 9001 registered. U.S. manufacturing.<br />
Booth 1021 (10 x 10)<br />
Titertek Instruments produces a variety of products for liquid handling and microplate<br />
handling and processing. In its Huntsville, Alabama facility, Titertek produces microplate<br />
stackers, dispensers, washers, and custom assay processors as well as semi-automated<br />
liquid handlers for sample transfer and primary processing. Microplate stackers are sold<br />
both with Titertek’s own equipment as well as integrated to a variety of third party devices.<br />
Booth 835 (10 x 10)<br />
Titian Software develops IT systems for the life science industries, specializing in software<br />
that manages the samples used in drug discovery. Our clients use our products to improve<br />
the effi ciency, throughput and data integrity of the sample storage and preparation<br />
processes that feed their vital research functions.<br />
Our Mosaic software suite manages libraries of compounds and reagents through<br />
all stages of the drug discovery process. It incorporates inventory tracking, workfl ow<br />
management, sample ordering and the integration of robotic workstations from leading<br />
vendors.<br />
291<br />
EXHIBITORS
Tomtec<br />
1000 Sherman Avenue<br />
Hamden, Connecticut 06514<br />
(203) 281-6790<br />
www.tomtec.com<br />
Torcon Instruments, Inc.<br />
22301 South Western Avenue Building 105<br />
Torrance, California 90501<br />
(310) 320-7313; (310) 618-0143 fax<br />
rkg@torconinstruments.com<br />
www.torconinstruments.com<br />
TradeWinds Direct, Inc.<br />
10555 86th Avenue<br />
Pleasant Prairie, Wisconsin 53158<br />
(888) 323-3585; (262) 605-3262 fax<br />
sales@tradewindsdirect.com<br />
www.twdinc.com<br />
Tricontinent<br />
12555 Loma Rica Drive<br />
Grass Valley, California 95945<br />
(530) 273-8888; (530) 273-2586 fax<br />
liquidhandling@tricontent.com<br />
www.tricontinent.com<br />
TTP LabTech<br />
Melbourne Science Park<br />
Cambridge Road<br />
Royston, Herts SG8 6EE<br />
United Kingdom<br />
sales@ttplabtech.com<br />
www.ttplabtech.com<br />
Union Biometrica, Inc.<br />
35 Medford Street, Suite 101<br />
Somerville, Massachusetts 02143<br />
(617) 591-1211; (617) 591-8388 fax<br />
sales@unionbio.com<br />
www.unionbio.com<br />
Booth 811 (10 x 30)<br />
Tomtec is a leading provider of innovative automated solutions for drug discovery<br />
research—96 & 384 well pipettors and instruments for microplate washing, sealing and<br />
storage—supporting a wide range of applications including HTS, SPE and Genomics.<br />
Tomtec continues to enhance existing products and introduce new technology to meet the<br />
changing needs of research. Tomtec has introduced a number of innovative instruments<br />
over the years including the Harvester96, Quadra96 and the newest—the Autogizer.<br />
Tomtec will display new instruments for use in cell-based assays, bioanalysis and<br />
genomics/proteomics: Quadra 3NS, The Tower, and SPExpress.<br />
Booth 211 (10 x 10)<br />
Torcon Instruments has been designing and manufacturing equipment to automate<br />
procedures in the microplate format since 1984. Personnel at Torcon have designed<br />
or been on the design team for the fi rst dual wavelength Optical Density Reader<br />
for microplates, the fi rst Fluorometer for microplates, and the fi rst Luminometer for<br />
microplates.<br />
Booth 1327 (10 x 10)<br />
TradeWinds Direct, Inc. is dedicated to the design and manufacture of high quality and<br />
innovative laboratory products in response to our customers’ needs. Our specialties<br />
include: Laser-Etched Ceramic Bar Codes, 1D/2D, Vials/Tubes/Slides Automated Laser<br />
Bar Coding Systems Automated Capping/Uncapping Systems, Screw/Crimp/Plug Caps,<br />
Standalone/ Integrated Custom Design/Manufacture of Plastic Glass and Metal Labware/<br />
Components Unique Standard Labware Products.<br />
Booth 1019 (10 x 10)<br />
TriContinent, an ISO 9001-certifi ed company, manufactures precise, reliable and affordable<br />
liquid handling instruments and devices, specializing in custom-made OEM products for<br />
clinical and biotechnology applications. TriContinent manufacturers OEM syringe pumps.<br />
Our pumps, whether standard, custom or modifi ed are designed for Low Cost, Quality<br />
and Application Optimization. TriContinent has established relationships with the most<br />
knowledgeable and successful clinical diagnostic and biotech companies in the world.<br />
Booth 1525 (10 x 30)<br />
TTP LabTech supplies laboratory-scale instrumentation and aut<strong>omation</strong> for the healthcare,<br />
biotech and pharmaceutical sectors. Its products include: comPOUND, a high-density,<br />
modular and scalable storage system, refrigerated to -20˚C; Mosquito, a low volume<br />
(50–1200nl) pipettor which uses disposable pipettes for zero cross-contamination; and<br />
Acumen Explorer, a laser-based fl uorescent microplate cytometer providing fast, multiparameter<br />
analysis for cell-based assays.<br />
Booth 938 (10 x 10)<br />
Union Biometrica provides automated systems for sorting/dispensing large (40–1,500<br />
microns) beads, such as those used in on-bead combinatorial assays, to facilitate<br />
experiment design for HTS compound screening and assay development. Based on size,<br />
optical density, and fl uorescent characteristics, COPAS instruments sort target beads<br />
into multiwell plates using a non-destructive sorting mechanism. This technology is also<br />
utilized for sorting of viable large cells, cellular aggregates, and small model organisms<br />
(C.elegans, Drosophila, zebrafi sh).<br />
292
United Chemical Technologies, Inc.<br />
2731 Bartram Road<br />
Bristol, Pennsylvania 19007<br />
(800) 385-3153; (215) 785-1226 fax<br />
www.unitedchem.com<br />
USA Scientific, Inc.<br />
P.O. Box 3565<br />
Ocala, Florida 34478<br />
(800) 522-8477; (352) 351-2057 fax<br />
infoline@usascientific.com<br />
www.usascientific.com<br />
V&P Scientific, Inc.<br />
9823 Pacific Heights Boulevard, Suite T<br />
San Diego, California 92121<br />
(858) 455-0643; (858) 455-0703 fax<br />
azabrocki@vp-scientific.com<br />
www.vp-scientific.com<br />
Veeco Instruments Inc.<br />
2650 East Elvira Road<br />
Tucson, Arizona 85706<br />
(520) 741-1044; (520) 294-1799 fax<br />
info@veeco.com<br />
www.veeco.com<br />
Velocity11<br />
435 Acacia Avenue<br />
Palo Alto, California 94306<br />
(650) 846-6500; (650) 846-6520 fax<br />
info@velocity11.com<br />
www.velocity11.com<br />
VICI Valco Instruments<br />
P.O. Box 55603<br />
Houston, Texas 77255<br />
(713) 688-9345; (713) 688-3948 fax<br />
valco@vici.com<br />
www.vici.com<br />
Booth 1501 (10 x 10)<br />
United Chemical Technologies is a major competitor in the fi eld of silica based solid phase<br />
extraction technology. We offer a selection of over 50 functionalized silica gels and a wide<br />
range of polymeric resins which can be packed into cartridges, fl ash chromatography<br />
columns, and plates. We carry both 96- and 48- well plates, which are compatible with<br />
many liquid handling systems. UCT supports all of these products with outstanding<br />
technical assistance.<br />
Booth 729 (10 x 10)<br />
USA Scientifi c provides solutions for sample handling, testing, storage, and tracking.<br />
Products on display include: TipOne ® manual and aut<strong>omation</strong> tips, Micronic ® racked tubes<br />
and sealing caps, real-time PCR fi lm and plates; 384- and 1536-well plates, freezer racks<br />
and labels, Opticell ® cell culture systems, and more.<br />
Booth 826 (10 x 20)<br />
V&P Scientifi c makes Pin Tools for most robotic systems to transfer nanoliter through<br />
microliter volumes from microplates to membranes, other microplates, agar or glass slides.<br />
We make pin tools for 24, 48, 96, 384 standard and deep well microplates, and even 1536<br />
microplates. V&P also invented the fi rst practical method to stir or aerate the contents of<br />
individual microwells with our Alligator Tumble Stirrers and Levitation Stirrers, and a simple<br />
method of keeping particulates in suspension while pipetting.<br />
Booth 1012 (10 x 10)<br />
Veeco Instruments Inc. is a worldwide leader in metrology tools for the scientifi c/research<br />
and industrial markets. Our newest product, the Oasis Protein Crystal Imaging System ,<br />
leverages Veeco’s industrial metrology experience into structural proteomics. This optical,<br />
high throughput screening tool provides unparalleled productivity and ease of use. Global<br />
sales and service offi ces are located throughout the United States, Europe, Japan and Asia<br />
Pacifi c. Additional information on Veeco can be found at www.veeco.com.<br />
Booth 1133 (20 x 30)<br />
Velocity11 is the leading manufacturer of aut<strong>omation</strong> platforms and benchtop<br />
instrumentation for laboratory processes. Velocity11’s suite of innovative products and<br />
commitment to service provide a winning combination for complete aut<strong>omation</strong> solutions.<br />
To see how we provide the fl exibility to meet your needs today and in the future, please<br />
stop by our booth or contact a Velocity11 sales representative.<br />
Booth 807 (10 x 20)<br />
For over 30 years, VICI ® Valco Instruments Co. Inc. has been the leading designer and<br />
manufacturer of valves and fi ttings for precision analytical, biomedical, and biocompatible<br />
instrumentation. Newest products for proteomics and genomics include: Cheminert ®<br />
CN2 Nanoinjector, optimized for nanovolume fl ow rates; Cheminert ® C2NH 10,000 psi<br />
injectors; and Cheminert ® C2NX 20,000 psi injectors.<br />
293<br />
EXHIBITORS
Waters Corporation<br />
34 Maple Street<br />
Milford, Massachusetts 01757<br />
(508) 478-2000; (508) 872-1990 fax<br />
info@waters.com<br />
www.waters.com<br />
Watson-Marlow Bredel Pumps<br />
37 Upton Drive<br />
Wilmington, Massachusetts 01887<br />
(978) 658-6168; (978) 658-0041 fax<br />
support@wmbpumps.com<br />
www.watson-marlow.com<br />
Whatman<br />
9 Bridewell Place<br />
Clifton, New Jersey 07014<br />
(973) 773-5800; (973) 773-0168 fax<br />
info@whatman.com”<br />
www.whatman.com<br />
White Carbon<br />
Melborun Science Park<br />
Melbourn, Royston<br />
Hertfordshire SG8 6EE<br />
United Kingdom<br />
44 1763 266606; 44 1763 262495 fax<br />
info@white-carbon.com<br />
www.white-carbon.com<br />
Zinsser Analytic GmbH<br />
Eschborner Landstr. 135<br />
Frankfurt D-60489, Germany<br />
49 69 789 1060; 49 69 789 10680 fax<br />
info@zinsser-analytic.com<br />
www.zinsser-analytic.com<br />
Booth 1110 (10 x 10)<br />
Now that Waters is home to Micromass MS technologies, no other company can offer you<br />
a more comprehensive range of products and services. Columns and Chemistries. Mass<br />
Spectrometry. HPLC. Instrument Control and Information Management Software. Service<br />
and Support. Whatever your laboratory’s needs are, we can answer them all.<br />
Booth 839 (10 x 10)<br />
Watson-Marlow Bredel, the world’s largest manufacturer of peristaltic pumps, will be<br />
displaying a wide range of instrument-quality OEM pumps. Customization services are<br />
available so that standard pumps can be optimized to best suit almost any application.<br />
The pumps are contamination-free and their simple design handles almost any fl uid<br />
reliability. Also featured will be PumpSil brand Silicone tubing with Lasertraceability for<br />
FDA approved applications.<br />
Booth 433 (10 x 10)<br />
Whatman is an international leader in separation technology manufacturing an extensive<br />
range of fi ltration products for high throughput sample preparation. In addition to standard<br />
collection and assay plates, we offer an extensive range of specialized membranes and<br />
medias encapsulated in multiwell fi lter plates of various well densities and volumes. Also<br />
available is a service for custom products and private labeling.<br />
Booth 1034 (10 x 10)<br />
White Carbon brings software-based solutions to life sciences. Its Pathways workfl ow<br />
system crosses the boundary between conventional LIMS systems and workcell control<br />
software, offering unrivalled integration and analysis capabilities. Pathways can be used to<br />
develop workfl ow systems rapidly and incrementally, enabling controlled process evolution<br />
and the capture of best practice.<br />
Booth 1534 (10 x 10)<br />
Zinsser Analytic is supplying a vast range of sophisticated systems and solutions for<br />
applications in modern drug discovery, combinatorial chemistry, screening and synthesis,<br />
as well as standard laboratory aut<strong>omation</strong>: LISSY- fast and precise liquid handling,<br />
LIPOS- automatic liquid and powder dispensing with powder pipette for variable deliveries<br />
of dry materials (also available with integrated microwave), CALLI- calibrating of vials<br />
and weighing of powders and liquid compounds (available with “intelligent” sample<br />
management system for “hit-picking” etc.—CALLI-plus).<br />
Zymark Corporation See Caliper Technologies Corporation on page 256.<br />
294
NOTES<br />
295
NOTES<br />
296
SHORT COURSES<br />
Sunday, February 1, 2004<br />
Barcode Technology – Page 298<br />
Economic Justification of Laboratory Aut<strong>omation</strong> – Page 298<br />
Emerging IT for the Laboratory – Page 298<br />
Introduction to High Throughput Screening – Page 298<br />
Introduction to Laboratory Aut<strong>omation</strong> – Page 299<br />
Introduction to LabView – Page 299<br />
Introduction to Molecular Biology – Page 299<br />
Mass Spectrometry Instrumentation & High Throughput Analysis – Page 299<br />
Microarrays & Their Applications – Page 300<br />
Migrating From VB6 to VB.Net – Page 300<br />
Sunday, February 1 and Monday, February 2: Two-Day Courses<br />
Getting Started With Excel and VBA in the Laboratory – Page 300<br />
Microfluidics I/II – Page 300<br />
Monday, February 2, 2004<br />
Biostatistics – Page 301<br />
Introduction to Databases in the Laboratory – Page 301<br />
Introduction to Laboratory Aut<strong>omation</strong> – Page 301<br />
LIMS in the Organization – Page 301<br />
Mass Spectrometry in Proteomics & Drug Discovery – Page 302<br />
Mathematica – Page 302<br />
Molecular Diagnostic Aut<strong>omation</strong> – Page 302<br />
Patent Law – Page 302<br />
Pharmacogenomic Aut<strong>omation</strong> – Page 303<br />
Trajectory of Clinical Laboratory Aut<strong>omation</strong> – Page 303<br />
297<br />
SHORT COURSES
Sunday, February 1, 2004<br />
Barcode Technology Room B1<br />
Andrew Zosel<br />
Microscan Systems, Inc.<br />
Renton, Washington<br />
azosel@microscan.com<br />
Niels Wartenberg<br />
Microscan Systems, Inc.<br />
Renton, Washington<br />
nwartenberg@microscan.com<br />
This course will address the information requirements needed for the design of an automated identification system.<br />
Examples will include patient, tray and reagent identifiers. Linear, stacked and 2D codes will be covered along with<br />
their advantages and disadvantages.<br />
Economic Justification of Laboratory Aut<strong>omation</strong> Room C1<br />
Douglas Gurevitch<br />
University of California, San Diego<br />
San Diego, California<br />
dgurevitch@yahoo.com<br />
This course is designed for anyone interested in analyzing the costs and justifications of laboratory aut<strong>omation</strong>,<br />
from laboratory scientist or manager to financial officer needing an introduction into laboratory financial issues.<br />
The goal of this course is to introduce students to the terminology and methods used in the economic analysis of<br />
laboratory aut<strong>omation</strong>.<br />
Emerging IT for the Laboratory Room B3<br />
Burkhard Schäfer<br />
Burkhard Schäfer Software and Networks<br />
Mainz, Germany<br />
bs-sc@bubusoft.com<br />
Torsten A. Staab<br />
Los Alamos National Laboratory<br />
Los Alamos, New Mexico<br />
tstaab@lanl.gov<br />
This short course will provide an overview of emerging IT (Information Technology) that is applicable to R&D labs in<br />
the pharmaceutical, biotech, and clinical sectors. More specifically, this course will provide managers, IT decision<br />
makers, and engineers with the latest IT trends, introduce new and emerging IT, and discuss their pros and cons.<br />
Live product demos of the latest software application development, system and data modeling, database, and<br />
Internet technologies will also be included.<br />
Introduction to High Throughput Screening Room J3<br />
Carol A. Homon<br />
Boehringer-Ingelheim Pharmaceuticals, Inc.<br />
Ridgefield, Connecticut<br />
chomon@rdg.boehringer-ingelheim.com<br />
Richard M. Nelson<br />
Boehringer-Ingelheim Pharmaceuticals, Inc.<br />
Ridgefield, Connecticut<br />
rnelson1@rdg.boehringer-ingelheim.com<br />
This introductory course is designed for professionals new to the field of high throughput screening as well as<br />
those looking to get an overview of the current state of the art. The course will review the principles, strategy,<br />
and technology employed by successful screening operations in the pharmaceutical industry. Specific examples<br />
and case studies will be used to illustrate the application of laboratory aut<strong>omation</strong>, assay biochemistry, and data<br />
analysis in the HTS environment and to highlight important tips and pitfalls that can have significant effects on<br />
screen quality and productivity.<br />
298
Introduction to Laboratory Aut<strong>omation</strong> Room J2<br />
Steven D. Hamilton<br />
Sanitas Consulting<br />
Boulder, Colorado<br />
s.d.hamilton@pobox.com<br />
Gary W. Kramer<br />
National Institute of Standards<br />
and Technology<br />
Gaithersburg, Maryland<br />
gkramer@enh.nist.gov<br />
299<br />
Mark F. Russo<br />
Bristol-Myers Squibb Co.<br />
Princeton, New Jersey<br />
russom@bms.com<br />
This course is designed for those seeking an introduction to and overview of the field of laboratory aut<strong>omation</strong>,<br />
including workstations, integrated systems, microtechnology and informatics. Methods of planning and executing<br />
successful and effective aut<strong>omation</strong> projects, as well as the drivers, costs and benefits of implementing laboratory<br />
aut<strong>omation</strong> will be discussed.<br />
Introduction to LabView Room C4<br />
Richard M. Brueggman<br />
Data Science Aut<strong>omation</strong>, Inc.<br />
Canonsburg, Pennsylvania<br />
rmb@DSAut<strong>omation</strong>.com<br />
Zachary Nelson<br />
National Instruments<br />
San Francisco, California<br />
zachary.nelson@ni.com<br />
Robin Carr<br />
Drexel University<br />
Philadelphia, Pennsylvania<br />
rcarr@coe.drexel.edu<br />
This course will demonstrate how to use National Instruments LabVIEW to develop custom laboratory aut<strong>omation</strong><br />
applications in an intuitive graphical development environment. Participants will use the Instrument I/O Assistant,<br />
DAQ Assistant, and several configurable “Express VIs” in LabVIEW 7.0 to acquire, analyze, and present data<br />
acquired from external instruments (RS-232 or GPIB / IEEE-488.2) and plug-in data acquisition cards. The Vision<br />
Assistant and Motion Assistant will also be used to configure image analysis and motion control tasks. Saving data<br />
to files and monitoring and controlling experiments over the network using a web browser will also be discussed.<br />
Introduction to Molecular Biology Room F<br />
Marianne Manchester<br />
The Scripps Research Institute<br />
La Jolla, California<br />
marim@scripps.edu<br />
Anette Schneemann<br />
The Scripps Research Institute<br />
La Jolla, California<br />
aschneem@scripps.edu<br />
This course is designed for individuals who have had little or no training in molecular biology and desire to acquire<br />
a working knowledge of the fundamental concepts and their applications. The course will cover basic processes<br />
such as DNA, RNA and protein synthesis, recombinant DNA technology, manipulation of gene expression, directed<br />
mutagenesis and protein engineering. It will also introduce the most important experimental techniques employed<br />
in this field. The course format will be informal and while no prior knowledge of molecular biology is required, some<br />
background in basic general science will be beneficial.<br />
Mass Spectrometry Instrumentation & High Throughput Analysis Room B2<br />
Nadja Cech<br />
University of North Carolina<br />
Greensboro, North Carolina<br />
nbcech@uncg.edu<br />
Mike Greig<br />
Pfizer Global R&D<br />
San Diego, California<br />
michael.greig@pfizer.com<br />
Gary Siuzdak<br />
The Scripps Research Institute<br />
La Jolla, California<br />
siuzdak@scripps.edu<br />
This course is designed to acquaint the student with many of the mass spectrometry systems now available<br />
providing real world applications of different types of instruments as well as giving critical comparisons.<br />
Discussion will include a wide range of topics in the aut<strong>omation</strong> and application of mass spectrometry, including<br />
high throughput analysis, liquid chromatography MS, high resolution MS, data management, tandem mass<br />
spectrometry, and unknown identification.<br />
SHORT COURSES
Sunday, February 1, 2004<br />
Microarrays & Their Applications Room J4<br />
Claus Bo Vöge Christensen<br />
Danish Technical University<br />
Lyngby, Denmark<br />
cbc@mic.dtu.dk<br />
Martin Dufva<br />
Danish Technical University<br />
Lyngby, Denmark<br />
mdu@mic.dtu.dk<br />
The course will illustrate the fundamental operating principles, fabrication possibilities and applications of DNAprotein-<br />
and small molecule arrays. Issues that will be covered include immobilization chemistries, choice of<br />
substrates, detection strategies, commercially available technologies and considerations for do-it-yourself systems.<br />
Migrating From VB6 to VB.Net Room B4<br />
Steven Hoffman<br />
Bristol-Myers Squibb Co.<br />
Princeton, New Jersey<br />
steven.hoffman@bms.com<br />
Mike Hudock<br />
University of Illinois<br />
Urbana, Illinois<br />
hudock@uiuc.edu<br />
This course is a primer on VB.Net for those programmers already familiar with VB6. The course will cover the major<br />
differences between the two development platforms and will focus on areas where common VB6 methodologies<br />
will cause problems in VB.Net code. This course is recommended for those individuals who are either planning to<br />
or who are starting to transition from VB6 to VB.Net.<br />
Sunday, February 1 and Monday February 2<br />
Getting Started With Excel and VBA in the Laboratory Room C2<br />
William Neil<br />
Bristol-Myers Squibb Co.<br />
Princeton, New Jersey<br />
william.neil@bms.com<br />
Erik Rubin<br />
Bristol-Myers Squibb Co.<br />
New Brunswick, New Jersey<br />
erik.rubin@bms.com<br />
Microsoft Excel is an indispensable tool for scientific calculations and laboratory data management and is<br />
ubiquitous in the scientific community. Further expanding the power of Excel is its support for the Visual Basic for<br />
Applications (VBA) programming language and a built-in integrated development environment. This two-day short<br />
course will introduce the fundamentals of the VBA core language, examine the interaction of VBA with Microsoft<br />
Excel, and explore the use of Excel and VBA programming to address basic laboratory aut<strong>omation</strong> challenges.<br />
Microfluidics I/II Room J1<br />
Jörg P. Kutter<br />
Danish Technical University<br />
Lyngby, Denmark<br />
jku@mic.dtu.dk<br />
R. Scott Martin<br />
Saint Louis University<br />
St. Louis, Missouri<br />
martinrs@slu.edu<br />
300<br />
Johan Nilsson<br />
Lund University<br />
Lund, Sweden<br />
johan.nilsson@elmat.lth.se<br />
The course will present an introduction to microliquid handling, discussing aspects of basic microfluidics, fluidic<br />
handling elements, microfabrication, integration and detection. An overview of the most important applications on<br />
these bio/chemical microdevices, from sample preparation and separation techniques to integrated biomedical<br />
processors, will be given.
Monday, February 2, 2004<br />
Biostatistics Room C1<br />
David Lansky<br />
Lansky Consulting, LLC<br />
Burlington, Vermont<br />
David@LanskyConsulting.com<br />
This course will introduce a collection of statistical ideas and show how they are used in assay design,<br />
development, and validation. The core concepts include: randomization, independence, distributions, models,<br />
residual plots, transformations, weights, outliers, experimental units, blocks, factorial designs, and variance<br />
components.<br />
Introduction to Databases in the Laboratory Room B1<br />
Steven Hoffman<br />
Bristol-Myers Squibb Co.<br />
Princeton, New Jersey<br />
steven.hoffman@bms.com<br />
Mike Hudock<br />
University of Illinois<br />
Urbana, Illinois<br />
hudock@uiuc.edu<br />
This hands-on course teaches database fundamentals as they pertain to a laboratory setting. The course will cover<br />
the differences between databases and other applications, most notably spreadsheets; the basic operations of<br />
tables, records, and fields; and an introduction to structured query language (SQL). This course is intended for<br />
individuals who are familiar with spreadsheets and who may want to migrate to a database and for individuals<br />
involved in the purchase of database driven systems who want a quick primer on the technology.<br />
Introduction to Laboratory Aut<strong>omation</strong> Room J2<br />
This is a repeat of the Sunday, February 1 course. See page 299 for the description.<br />
LIMS in the Organization Room B4<br />
Robert D. McDowall<br />
McDowall Consulting<br />
Bromley, Kent, United Kingdom<br />
R_D_McDowall@compuserve.com<br />
Laboratory Information Management Systems (LIMS) are used to administer sample information and collate the<br />
results from laboratory analyses to deliver reports of the work. The course will give a high level introduction to<br />
implementing a system in a laboratory and will focus on the areas where many projects fail. Topics include defining<br />
the system scope, writing the user requirements for the system, involving the users, testing the system and project<br />
risk management.<br />
301<br />
SHORT COURSES
Monday, February 2, 2004<br />
Mass Spectrometry in Proteomics & Drug Discovery Room B2<br />
Mike Greig<br />
Pfizer Global R&D<br />
San Diego, California<br />
michael.greig@pfizer.com<br />
Gary Siuzdak<br />
The Scripps Research Institute<br />
La Jolla, California<br />
siuzdak@scripps.edu<br />
302<br />
Tom Hollenbeck<br />
Genomics Institute of the Novartis<br />
Research Foundation<br />
San Diego, California<br />
thollenb@gnf.org<br />
Our course will focus on the present state of the art for mass spectrometry of proteins, small biomolecules and<br />
drug analysis. The ease with which mass spectrometry can be automated has brought it to the forefront as an<br />
analytical tool for protein characterization, drug discovery, pharmacokinetics and even disease diagnosis.<br />
Mathematica Room C4<br />
Paul Wellin<br />
Wolfram Research<br />
Champaign, Illinois<br />
wellin@wolfram.com<br />
This short course provides direct, hands-on experience with all of the basic features of /Mathematica/, including<br />
data analysis and manipulation. The course is designed primarily for people who are interested in becoming<br />
proficient /Mathematica/ users but who currently have little or no experience with the system. For additional<br />
information, go to www.wolfram.com/weg/courses/m100.html.<br />
Molecular Diagnostic Aut<strong>omation</strong> Room J4<br />
Patrick Merel<br />
Bordeaux University Hospital<br />
Bordeaux, France<br />
patrick.merel@chu-bordeaux.fr<br />
This course will review the most serious options of today for Nucleic Acid Extraction, Amplification Setup and<br />
Post-Amplification Detection Steps aut<strong>omation</strong>. New alternatives and new technologies will be reviewed, as well as<br />
low cost procedures for middle to high throughput molecular testing.<br />
Patent Law Room F<br />
John Wetherell<br />
Pillsbury Winthrop LLP<br />
San Diego, California<br />
jwetherell@pillsburywinthrop.com<br />
This short course will provide critical information for creating and maintaining a proprietary position for<br />
commercialization. The course will survey the various areas in intellectual property, such as patents and trade<br />
secrets and provide a fundamental background on the criteria which must be met in order to obtain a patent.<br />
Additional topics relate to various aspects of strategic planning, such as pursuing protection in foreign jurisdictions,<br />
and determining inventorship. This course does not require a technical background or prior experience with<br />
intellectual property.
Pharmacogenomic Aut<strong>omation</strong> Room J3<br />
Thomas Hartmann<br />
Amgen, Inc.<br />
Thousand Oaks, California<br />
thomash@amgen.com<br />
Paul Kayne<br />
Bristol-Myers Squibb Co.<br />
Princeton, New Jersey<br />
paul.kayne@bms.com<br />
This course is designed to introduce the theory and practice of pharmacogenomics. Pharmacogenetics is the<br />
study of genetic variation underlying differential response to pharmacological agents. The course will cover<br />
three aspects: the biology relevant to pharmacogenomics, assays used in pharmacogenomics, and methods of<br />
automating pharmacogenomics.<br />
Trajectory of Clinical Laboratory Aut<strong>omation</strong> Room B3<br />
Rodney Markin<br />
University of Nebraska Medical Center<br />
Omaha, Nebraska<br />
rmarkin@unmc.edu<br />
Mary Newcomb<br />
Lab-Interlink<br />
Omaha, Nebraska<br />
Today’s clinical laboratory operation will be unlikely to be successful without implementing clinical laboratory<br />
aut<strong>omation</strong> technology to improve laboratory productivity, manage resources, and maintain quality. Knowledgeable<br />
planning and implementation are key to successful aut<strong>omation</strong> applications. This introductory course is aimed at<br />
those considering automating their clinical laboratory operations and at those managing increasingly automated<br />
laboratories.<br />
303<br />
SHORT COURSES
NOTES<br />
304
INDEX<br />
3M Bioanalytical 249<br />
A<br />
Aarhaug, Thor Anders 27, 146<br />
AB Controls 249<br />
ABgene 249<br />
ABS, Inc. 249<br />
Adams, John A. 18, 85<br />
Adams, Monica 27, 146<br />
Adam, Shiraz 27, 152<br />
Adept Technology, Inc. 249<br />
Adhesives Research, Inc. 250<br />
Advion BioSciences, Inc. 250<br />
Aerotech, Inc. 250<br />
Affordable Aut<strong>omation</strong> Ltd 250<br />
Aflatooni, Nima 78<br />
AID-CORP 250<br />
Alanine, Alexander 23, 56<br />
Alderman, Edward 27, 147<br />
Alexander, John 142<br />
ALIO Industries 250<br />
Allegro Technologies 251<br />
Allen, Jeffrey 20, 113<br />
Allwardt, Arne 93<br />
Altekar, Maneessha 20, 65<br />
American Linear Manufacturers 251<br />
Amersham Biosciences Corp. 251<br />
Amur, Shashi 97<br />
Andersen, Peter E. 32, 183<br />
Anderson, Marcy 22, 116<br />
Andersson, Helene 19, 74<br />
Applied Biosystems 251<br />
Applied Mechatronics 251<br />
Applied Robotics, Inc. 252<br />
Applied Scientific Instrumentation 252<br />
Apricot Designs, Inc. 252<br />
Armstrong, Jason 20, 128<br />
Arnold, Don 48<br />
Arnstein, Larry 23, 139<br />
ARTEL 252<br />
Arumugam, Prabhu U. 20, 128<br />
Asensio, Francisco 38, 221<br />
Ashman, Keith 17, 108<br />
Association for Laboratory<br />
Aut<strong>omation</strong> 252, 316, 317, 320<br />
Astech Projects Ltd. 252<br />
Aurora Discovery, Inc. 253<br />
Axygen Scientific 253<br />
Azzaoui, Kamal 65<br />
B<br />
Backhouse, C. J. 71<br />
Baechler, Guido 20, 112<br />
Bagwell, Hunter 22, 116, 117<br />
Baker, Jill 17, 69<br />
Baird, Cheryl 129<br />
Bal Seal Engineering 253<br />
Balch, Gina 33, 190<br />
Bagallo, Chris 27, 34, 147, 192<br />
Barnstead International/STEM 253<br />
Baron, Gino 70<br />
Batalov, Sergei 118<br />
Batchelor, James 28, 34, 156, 200<br />
Bauer, H. 31, 179<br />
Bayer, Travis S. 103<br />
BD Biosciences 253<br />
Becker, Michael 17<br />
Beckman Coulter, Inc. 242, 253<br />
Beech, Sarah 38, 126, 223<br />
Behnke, James 78<br />
Belgoviskiy, Alexander 66<br />
Belgrader, Phillip 23, 78<br />
Belson, Adam 28, 157<br />
Beneke, Ralph 37, 213<br />
Benes, Vladimir 40, 234<br />
Benn, Neil 21, 54<br />
Bergholtz, Stine 29, 36, 161, 205<br />
Berhitu, Alex 27, 29, 34, 148, 161,<br />
192<br />
Berlin, Emily 27, 34, 148, 193, 201<br />
Bernard, Christopher 27, 149<br />
Betgovargez, Edna 38, 220<br />
Betz, Stefan 27, 150<br />
Beugelsdijk, Tony J. 16<br />
Bevan, Chris 18, 48<br />
Bharadwaj, Rajiv 17, 70<br />
Bhat, Naryan K. 32, 184<br />
Biagioli, Chris 36, 208<br />
Bialy, Laurent 28, 157<br />
Bier, Frank F. 22, 88, 105<br />
Big Bear Aut<strong>omation</strong> 254<br />
Binnie, Alastair 27, 149<br />
Bio-Tek Instruments, Inc. 254<br />
Bio Integrated Solutions 254<br />
Bi<strong>omation</strong> 254<br />
BioMedTech Laboratories, Inc. 254<br />
305<br />
BioMicroLab, Inc. 254<br />
Biondi, Sherri 79<br />
Biosero 255<br />
Biotage 255<br />
BioTX Aut<strong>omation</strong>, Inc. 255<br />
Bischoff, Rainer 32, 180<br />
Bishop, Richard 87<br />
Bishop-Wisecarver Corporation 255<br />
Blaga, Iuliu 77<br />
Blake, Julie 97<br />
Blake, T. M. 83<br />
Blanchard, Mary M. 33, 190<br />
Blodgett, Jason 27, 147<br />
Blyth, Jeff 44<br />
BMG Labtechnologies, Inc. 255<br />
Boge, Annegret 27, 37, 151, 211<br />
Boillat, Marc 30, 31, 167, 173<br />
Bolanos, Ben 134<br />
Bologa, Cristian 53<br />
Bölstler, Frank 140<br />
Bonanno, Jeffrey B. 86<br />
Bonner, M. Roxane 120<br />
Boone, Travis 21, 89<br />
Boorman, Gary A. 56<br />
Borgen, Tine 29, 36, 161, 205<br />
Bornhop, Darryl J. 20, 32, 183<br />
Boston Biomedica, Inc. 255<br />
Bouhelal, Rochdi 65<br />
Boulin, Christian 40, 234<br />
Bowen, Wayne 27, 33, 34, 37, 39,<br />
151, 187, 193, 217, 230<br />
Bowlby, Evelyn E. 40, 232<br />
Boyd, Brian 39, 229<br />
Boyer, Scott 31, 36, 37, 40, 99, 177,<br />
210, 212, 234<br />
Bradley, Mark 19, 20, 27, 28, 51,<br />
150, 157<br />
Bradshaw, John 24, 141<br />
Brand, Randall 82<br />
Brandel 256<br />
Brauner, Erik 53<br />
Bray, Terry 66<br />
Brideau, Christine 27, 152<br />
Briggs, Erica 22, 136, 137<br />
Brinkmann Instruments Inc. 256<br />
Brinkman, Steve 35, 199<br />
Brook, Carlton 69<br />
Brooks Aut<strong>omation</strong>, Inc., Life<br />
Sciences Group 256<br />
INDEX
Brounstein, Kevin 78<br />
Brown, Clifford 63<br />
Brown, Mike 39, 226<br />
Brown, Sven 124<br />
Browning, Brent 63<br />
Brueggman, Richard M. 299<br />
Bruner, Jimmy 28, 38, 68, 152, 221<br />
Buckler, David 35, 203<br />
Budd, John 34, 39, 193, 230<br />
Buehrer, Lenore 30, 168<br />
Bullen, Andrew 27, 149<br />
Burdett, Laurie 127<br />
Burgess, Kevin 16, 45<br />
Burke, Julian F. 37, 125, 216<br />
Burley, Stephen K. 86<br />
Burns, Christine 39, 227<br />
Burns, Norman 77<br />
Cai, Hong 100<br />
C<br />
Cai, Sui Xiong 126<br />
Calamunci, Rob 35, 202<br />
Caliper Technologies Corporation<br />
242, 256<br />
Callamaras, Nick 124<br />
Campbell, Dana 99<br />
Campbell, Jesse 28, 153<br />
Carlo, Anthony 20, 64<br />
Carr, Robin 299<br />
Carreira, Erick 16, 45<br />
Cary, Robert B. 19, 22, 100<br />
Cathey, Cheryl 33, 188<br />
Cech, Nadja 299<br />
The Center for Biophysical Sciences<br />
and Engineering 256<br />
Chait, Arnon 66<br />
Chan, Daniel W. 17, 108<br />
Chan-Hui, Po-Ying 89<br />
Chang, Julie 28, 153<br />
Chatrathi, Madhu Prakash 28, 154<br />
Chatterjee, Moneesh 27 149<br />
Che, Jian 104<br />
Cheetham, Janet 135<br />
Chen, Lilly 89<br />
Chen, Wensheng 28, 157<br />
Cherry, James M. 32, 184<br />
Cheu, Melissa 28, 154<br />
Cheung, Peter 87<br />
Chipman, Stewart 18, 135<br />
Chiu, Daniel 19, 73<br />
Chiu, Mark 36, 209<br />
Choi, Bernard 21, 53<br />
Chow, Andrea 79<br />
Chow, Eva Y. W. 40, 232<br />
Christensen, Claus Bo Vöge 129,<br />
300<br />
Christensen, Louise Dahl 129<br />
Christmann, Alexander 105<br />
Chromagen, Inc. 257<br />
Chu, Chi-Tai 29, 164<br />
Chudyk, Jon 22, 104<br />
Chui, Buena 120<br />
Chun, Lawrence 28, 155<br />
Ciumasu, Ioan M. 35, 203<br />
Clark, Kelly M. 35, 200<br />
Clark, Liz 65<br />
Clark, Robin 28, 155<br />
Clarke, Glenn 38, 221<br />
Clicq, David 70<br />
Cliffel, David 20, 74<br />
Cloutier, Normand 21, 67<br />
Code Refinery 257<br />
Cohen, Michael J. 28, 153<br />
Cole, Kate 29, 163<br />
Colehan, James 125<br />
Collett, Jim 103<br />
Collingsworth, Michael 34, 194<br />
Coma, Isabel 65<br />
Comita, Paul B. 124<br />
Computype, Inc. 257<br />
Cong, Mei 27, 147<br />
Conner, Denise 28, 155<br />
Cook, Matthew 19, 27, 28, 127,<br />
151, 155<br />
Cooke, Michael 118<br />
Cooks, R. G. 83<br />
Cooper, David 68<br />
Cope, Tristan 27, 151<br />
Cornett, Mary 85<br />
Corning Incorporated 257<br />
Corso, Thomas 24, 81<br />
Cosenza, Larry 66<br />
Cosic, Janja 35, 203<br />
Costa, Jose 82<br />
Costantin, James 18, 124<br />
Cotter, Robert 17, 95<br />
Cowan, Chris 27, 28, 35, 147, 156,<br />
202<br />
Cox, J. Colin 22, 29, 31, 103, 164,<br />
165, 176<br />
Crabtree, H. John 18, 71<br />
Crittenden, Carole 28, 34, 156, 194<br />
Cromwell, Evan 18, 124<br />
Crosby, Renae 38, 221<br />
Cu, Matthew 38, 220<br />
306<br />
Cumme, G. A. 37, 215<br />
Cunningham, Brian 20, 129<br />
Cunningham, Michael L. 56<br />
Curtis, Richard H. 141<br />
CyBio AG 257<br />
Cyr, Doug 48<br />
D<br />
Daffertshofer, Martin 18, 62<br />
Dains, Katherine 28, 157<br />
Dandekar, Samir 39, 226<br />
Danker, Timm 60<br />
Dapprich, Johannes 19, 127<br />
Davies, Merrill L. 94<br />
Davidson, Colin 44<br />
Davis, Jim 18, 98<br />
Davis, Ronald W. 101<br />
Davisson, V. J. 83<br />
Dawson, Philip E. 21, 89<br />
deCODE genetics 258<br />
Dedeo, Anja 34, 196<br />
Del-Tron Precision Inc. 258<br />
Delaney, Edward 94<br />
DeLucas, Larry 21, 66<br />
Denoyer, Eric 90<br />
Dery, Olivier 28, 127, 155<br />
Desai, Sattu 84<br />
Desai, Tejal A. 31, 32, 178, 186<br />
Desmet, Gert 70<br />
Dettloff, Roger 135<br />
DeVoe, Don L. 22, 77<br />
Dextras, Philip 98<br />
Diamond, Scott L. 91<br />
Diaz-Mochon, Juan Jose 28, 157<br />
DiCesare, Joseph 36, 90, 204<br />
Dick, Lawrence 33, 73, 188<br />
Dickinson, William 104<br />
Digital Bio Technology, Inc. 258<br />
DigitalVAR, Inc. 258<br />
Dill, Killian 104<br />
Dillon, Deborah 82<br />
Dionex Corporation 258<br />
Discovery Partners International 259<br />
Dixon, James 28, 158<br />
Doktycz, Mitchel J. 104<br />
Döring, Klaus 37<br />
Dorner, Johann 29, 107, 140, 159<br />
Dorsett, David 17, 123<br />
Dotson, Crystal 119<br />
Drake, Doug 29, 34, 158, 194<br />
DRD Diluter Corporation 259<br />
Dreiss, Philipp 29, 107, 159
Dressendorfer, Paul 22<br />
Dressler, Sigmar 65<br />
Drewe, John 126<br />
Drews, Jürgen 12, 16, 23, 43<br />
Drug Discovery & Development 259<br />
Drug Discovery World 259<br />
Du, Ping 21, 29, 54, 159<br />
Du, Yi 92<br />
Dufresne, Claude 38, 221<br />
Dufva, Martin 20, 129, 300<br />
Dulle, Steve 33, 190<br />
Dumaual, Carmen 119<br />
Duncan, Wayne 29, 159<br />
Dunka, Louis 18, 111<br />
Dunne, Jude 39, 232<br />
Dunphy, Katherine 17, 68<br />
E<br />
E&K Scientific Products 260<br />
Eason, Paula Denney 39, 226<br />
Eastern Plastics Inc. 259<br />
Eberle, Klaus Günther 40, 234<br />
EDC Biosystems 260<br />
Ediger, Richard 90<br />
Edwards, Todd 29, 160<br />
Eendhuizen, Steven 29, 34, 161, 192<br />
Egeberg, Morten 29, 34, 161, 195<br />
Ehrentreich-Foerster, Eva 88<br />
Eidelberg, Boaz 34, 195<br />
Eklund, Sven 74<br />
Eksigent Technologies 260<br />
Ekström, Simon 80<br />
Elands, Jack 34, 194<br />
Elkin, Chris 35, 198<br />
Elling, John 20, 66<br />
Ellington, Andrew D. 29, 31, 32,<br />
103, 164, 165, 176, 183<br />
Ellman, Jonathan A. 20, 52, 91<br />
Ellson, Richard 19, 63<br />
ELMO Motion Control, Inc. 260<br />
Elseveir 260<br />
Emili, Andrew 31, 176<br />
Emptage, Mike 68<br />
Encynova 260<br />
Endress, Gregory A. 29, 160<br />
Engelstein, Marcy 34, 196<br />
Entzian, Kristin 33, 191, 39, 225<br />
Essen Instruments 261<br />
Everitt, Elizabeth 23<br />
Evotec Technologies 261<br />
Exatron Corporation 261<br />
F<br />
Faham, Malek 101<br />
Fang, Francine 28, 155<br />
Fang, Xiangming 91<br />
Fang, Xingwang 19, 34, 39, 162,<br />
196, 231<br />
Fanger, Neil 139<br />
Farinas, Javier 33, 39, 188, 232<br />
Farmen, Mark 119<br />
Farre, Cecilia 73<br />
Farrow, Roger 48<br />
Fathollahi, Bahram 79<br />
Feitelson, Jerry 91<br />
Feng, I-wei 135<br />
Ferentinos, Jerry 27, 152<br />
Fernandez, Jose A. 89<br />
Fernandez-Vina, Marcello 127<br />
Ferrari, Mauro 32, 182<br />
FIBERLite Centrifuge 261<br />
Fieweger, Kim 104<br />
Fillers, W. Steven 20, 29, 162<br />
Finlay, Cathy 28, 38, 68, 152, 221<br />
Finlay, Judith 21, 88<br />
Fisher Scientific 261<br />
Fitzgerald, Robert 30, 170<br />
Flynn, Julie 33, 189<br />
Fortin, Louis Jacques 27, 152<br />
Foshee, Melissa 37, 213<br />
Fragiadakis, Cheryl 22<br />
Frank, Michael 27, 150<br />
Frankenberger, Casey 37, 214<br />
Frechet, Jean 31, 174<br />
Frese, Ines 35, 203<br />
Friedlander, Thomas 29, 163<br />
Fritsch, Ingrid 128<br />
Fuchs, R. 31, 179<br />
Fung, Eric 19, 86<br />
G<br />
G&L Precision Die Cutting, Inc. 261<br />
Gabriel, Daniela 65<br />
Gaitan, Michael 79<br />
Gajovic-Eichelmann, Nenad 21,<br />
88, 105<br />
Gallagher, Sean 29, 163<br />
Ganjei, J. Kelly 30, 169<br />
Gao, Alice 29, 34, 164, 197<br />
Gao, Xia 19, 86<br />
Garcia, Carlos 29, 164<br />
Gardner, Ben D. 95<br />
Garner, Mark 86<br />
Garner, Toni 27, 148<br />
307<br />
Garyentes, Tina 17<br />
Gasior, Carlton 88<br />
Gaskin, Michael 120<br />
Gazzillo, Lisa 33, 186<br />
Geierstanger, Bernard 17<br />
General Data Company, Inc. 262<br />
Genetic Engineering News/Modern<br />
Drug & Discovery 262<br />
Genetix 238, 262<br />
Genmark Aut<strong>omation</strong> 262, 263<br />
Genomic Solutions 262<br />
GenoVision Inc. 264<br />
GenVault Corporation 264<br />
Gerlach, A. 30, 165<br />
Ghindilis, Andrey 22, 104<br />
Gibbs, Richard A. 27, 148<br />
Gil, Sonia 35, 38. 201, 219<br />
Gill, James 23, 61, 67<br />
Gillooly, David 36, 205<br />
Gilson, Inc. 264<br />
Girardi, Michael 37, 215<br />
Girod, Michel 65<br />
Glas-Col, LLC 264<br />
Godsey, James 17, 96<br />
Goertz, Patrick 29, 165<br />
Gold, Larry 17, 109<br />
Goldenberg, Andrew A. 31, 176<br />
Goldstein, Marc 37, 214<br />
Gologan, B. 83<br />
Gombocz, Erich A. 18, 60<br />
Goodacre, Roy 13<br />
Goode, Kate 35, 199<br />
Goodlett, David R. 22, 35, 90, 200<br />
Gordon, Steven 18, 97<br />
Gosalia, Dhaval N. 22, 91<br />
Gottschlich, Norbert 30, 165<br />
Gradl, Gabriele 20, 130<br />
Granchelli, Joseph 30, 166<br />
Grandsard, Peter 16, 24, 135, 142<br />
Gregg, Jeffrey 33, 189<br />
Greig, Michael 134, 299, 302<br />
Greiner Bio-One, Inc. 238, 264<br />
Griebel, Ralf 40, 234<br />
Griffin, David 30, 166<br />
Griffin, John H. 89<br />
Grigg, Ronald 32, 181<br />
Grimes, Craig 31, 178<br />
Gripenberg-Lerche, Christel 38, 39,<br />
220, 228<br />
Grossi, Erin 35, 202<br />
Grove, Geoffrey N. 27, 147<br />
Grunst, Terri 30, 35, 167, 202<br />
Guber, A. E. 30, 165<br />
INDEX
Gubler, Hanspeter 20, 65<br />
Guggenheimer, Kurtis 18, 98<br />
Gunic, Javorka 32, 38, 185, 224<br />
Gurevitch, Douglas 298<br />
Gurske, William 77<br />
Guss, Jeffrey 27, 149<br />
Gustafsson, Omar 76<br />
Gwynne, Penny 120<br />
H<br />
Haab, Brian 17, 82<br />
Haan, Nick 18, 61<br />
Habenbacher, Gerald 30, 171<br />
Haber, Carsten 30, 167<br />
Hahn, Mathew 17, 122<br />
Hahnenberger, Karen 48<br />
Halcome, Jennifer 30, 35, 168, 200<br />
Halley, George R. 30, 35, 168, 200<br />
Halloran, Stephen 39, 230<br />
Hamdan, Hasnah 20, 112<br />
Hamilton Company 264<br />
Hamilton, Steven D. 299<br />
Hamrick, David 66<br />
Hamstra, Alan 37, 214, 216<br />
Handran, Shawn 124<br />
Hardenbol, Paul 101<br />
Harper, Gavin 65<br />
Harrer, Bruce 22, 138<br />
Harris, David 63<br />
Hartmann, Michelle 86<br />
Hartmann, Thomas 303<br />
Hatcher, Sandra 33, 189<br />
Hawker, Charles 22, 24, 116<br />
Haynes, Paul A. 22<br />
Hayos, Jill 33, 189<br />
He, Qing 81<br />
Heineman, William 35, 199<br />
Heinitz, Wof-Dieter 38, 222<br />
Heinloth, Alexandra 23, 56<br />
Held, Paul 30, 168<br />
Helde, Paavo 62<br />
Helenius, Joni 38, 220<br />
Helfrich, John 30, 169<br />
HEMCO Corporation 265<br />
Hemmilä, Ilkka 39, 228<br />
Hencken, Ken 48<br />
Henkel, Joerg 88, 105<br />
Hensley, Joseph A. 35, 200<br />
Hensley, Paul 20, 130<br />
Herich, John 126<br />
Hermann, Terry 30, 34, 169, 197<br />
Herold, Chris 19<br />
Herold, David 30, 170, 172<br />
Herranz, Jesus 65<br />
Hessel, Volker 35, 203<br />
Hettich 265<br />
Heynen, Susanne 125<br />
Hilder, Emily 31, 174<br />
Hilhorst, Martijn 29, 161<br />
Hill, W. Adam 22, 67<br />
Hillegonds, Darren 30, 170<br />
Hilt, Lynn 30, 171<br />
Hines, Craig 35, 199<br />
Hird, Nicholas 20<br />
Hitti, Joseph 34, 192<br />
Hoang, Quoc 29, 34, 162, 196<br />
Hobbs, Steve 13, 33, 71, 187<br />
Hockett, Richard 23, 119<br />
Hoffman, John K. 85<br />
Hoffman, Randall 30, 171<br />
Hoffman, Steven 300, 301<br />
Hoffmann, W. 30, 165<br />
Hofmann, Glenn 65<br />
Hofstadler, Steven 17, 19, 96<br />
Hogenesch, John 118<br />
Hollenbeck, Tom 302<br />
Hölzel, Ralph 105<br />
Homon, Carol A. 37, 142, 215, 298<br />
Honisch, Ulrike 34, 198<br />
Hoover, Debra 29, 34, 164, 197<br />
Horn, A. 37, 215<br />
Howland, John 131<br />
Hrusovsky, E. Kevin 24<br />
Hsieh, Charles 106<br />
Hu, Tao 34, 193<br />
Huang, Lei 91<br />
Huber, David 21, 132<br />
Hudock, Mike 300, 301<br />
Hudson Control Group, Inc. 265<br />
Huitt, Gerry 30, 168<br />
Humphries, David 35, 198<br />
Hunike-Smith, Scott 18<br />
Hunkapiller, Kathryn 97<br />
Hunter, Joel 27, 152<br />
Hurd, Douglas 31, 175<br />
Hurskainen, Pertti 39, 228<br />
IDBS Inc. 239, 265<br />
IKO Intenational, Inc. 265<br />
ILS Innovative Labor Systeme 266<br />
INA Linear Technik, a division of INA<br />
USA Corp. 266<br />
Innovadyne Technologies, Inc. 266<br />
Innovative Microplate 266<br />
I<br />
308<br />
Invetech Instrument Development<br />
and Manufacture 266<br />
Irwin, Richard D. 56<br />
ISC BioExpress 266<br />
Ito, Ralph K. 29, 160<br />
J-Kem Scientific, Inc. 267<br />
Jacobs, Michael 28, 154<br />
Jacobson, Dawn Marie 30, 172<br />
Jaffe, Syrus 98<br />
Jahn, Andreas 79<br />
J<br />
Jain, Maneesh 101<br />
Jain, Suresh 73<br />
James, Anthony 44<br />
Jannetto, Paul 121<br />
Jardemark, Kent 73<br />
Jehle, Heinrich 62<br />
Jencons Scientific, Inc. 267<br />
Jensen, Klavs 19, 50<br />
Jewell, David W. 85<br />
Jiang, Lihua 92<br />
Jiang, Sky 37, 125, 216<br />
Jiang, Zhiming 99<br />
Johnson, Bruce 78<br />
Johnson, Diane 35, 199<br />
Johnson, Jason 36, 208<br />
Johnson, Kent 35, 36, 202, 208<br />
Jones, Eric 23, 139<br />
Jones, Jay Burton 22, 116, 117<br />
Jones, Mike 35, 199<br />
Jones, Sarah 127<br />
Jordan, Lynn 35, 200<br />
Joshi, Sanjaya 21, 35, 133, 200<br />
Jouan Robotics 267<br />
Journal of the Association for<br />
Laboratory Aut<strong>omation</strong><br />
(JALA) 242, 260<br />
Jovanovich, Stevan 22, 77<br />
Julabo USA, Inc. 267<br />
Jun-Air, USA 267<br />
K<br />
Kabilan, Satyamoorthy 44<br />
Kane, Jeff 35, 201<br />
Karcher, Brent 24, 94<br />
Karg, Jeffrey 20, 21, 131<br />
Karlin-Neumann, George 101<br />
Karlsruhe, Forschungszentrum 30,<br />
165<br />
Karlsson, Mattias 73<br />
Karnik, R. 68
Kashem, Mohammed 142<br />
Kasibhatla, Shailaja 126<br />
Kath, Gary 38, 221<br />
Kaur, Jaskiran 33, 190<br />
Kayne, Paul 21, 303<br />
Kazan, Greg 27, 36, 147, 206<br />
Kellard, Libby 27, 34, 38, 147, 201,<br />
219<br />
Kelleher, Neil 23, 92<br />
Kelley, Brian P. 66<br />
Kelly, Michele 35, 199<br />
Kendro Laboratory Products 267<br />
Kephart, Dan 30, 35, 167, 202<br />
Khovananth, Kevin 36, 208<br />
Kiechle, Frederick 18, 111<br />
Kim, Hidong 84<br />
Kim, Joong Hyun 39, 172<br />
King, Greg 38, 221<br />
Kirkwood, Sandra 119<br />
Kishbaugh, Alan 35, 36, 202, 208<br />
Kloehn Company 268<br />
KMC Systems, Inc. 268<br />
Knaide, Tanya R. 141<br />
Knebel, Günther 30, 34, 62, 165, 197<br />
Knight, Benjamin 73<br />
Knoll, Gerald 23, 140<br />
Knott, Thomas 60<br />
Kochins, John 18<br />
Koehler, Jeffrey 33, 187<br />
Kolb, Gunther 35, 203<br />
Kolossov, Evgueni 29, 158<br />
Kopacz, Kristopher 35, 203<br />
Kopf-Sill, Anne 24, 69<br />
Köprunner, M. 31, 179<br />
Korytko, Andrew 28, 153<br />
Koster, Emile 27, 34, 148, 192<br />
Kotturi, Paul 135<br />
Kovanen, Satu 38, 220<br />
Kramer, Gary W. 32, 182, 299<br />
Krämer, Petra M. 35, 203<br />
Kratochwil, K. 31, 179<br />
Kreil, G. 31, 179<br />
Kricka, Larry 17, 109<br />
Kubischta, Duane 35, 204<br />
Kumar, Akhauri P. 29, 107, 159<br />
Kumar, Mohit 32, 185<br />
Kumar, Tharun 53<br />
Kuoni, Andreas 31, 173<br />
Kuranda, Michael 73<br />
Kureshy, Fareed 99<br />
Kutter, Jörg P. 18, 22, 76, 300<br />
Kuzdzal, Scott 36, 204<br />
Kymäläinen, Virpi 36, 205<br />
LABCON North America 268<br />
Labcyte 268<br />
LabVantage Solutions, Inc. 268<br />
L<br />
Lacher, Nathan A. 76<br />
Lachnit, Wilhelm 124<br />
Laffite, Bryan 28, 152<br />
Lage, Michelle 39, 224<br />
Lake, Steve 22, 136<br />
Laleli-Sahin, Elvan 24, 121<br />
Lam, Kit S. 46<br />
Lamberg, Arja 36, 205<br />
Lange, Fred 31, 36, 173, 205<br />
Lansing, Manfred 37, 213<br />
Lansky, David 301<br />
Laris, Casey 27, 147<br />
Larsen, Niels B. 32, 183<br />
Lathrop Engineering, Inc. 269<br />
Laurell, Thomas 80<br />
Lawrence, Nathan 22, 78<br />
Lawrence Berkeley National<br />
Laboratory 269<br />
Laycock, John D. 33, 40, 189, 233<br />
Le, Thomas 33, 190<br />
Le Gall, Annick 28, 155<br />
LEAP Technologies 269, 270<br />
Lebl, Michael 16<br />
Ledesma, Annalee 120<br />
Lee, Hookeun 35, 200<br />
Lee, Ka Wah 32, 186<br />
Lee, Mei-Ching 44<br />
Lee, Stephen C. 32, 182<br />
Lee, Terry D. 24, 81<br />
Leeker, Russell 133<br />
Lehman, Alan 46<br />
Leinen, Kyle 106<br />
Lekin, Tim 85<br />
Lemon, Andrew 29, 158<br />
Lennon, Mark 65<br />
Lewis, Rob 33, 187<br />
Leytner, Svetlana 35, 202<br />
Li, Chunqin 78<br />
Li, Ella 40, 232<br />
Li, Leping 56<br />
Li, Michelle W. 76<br />
Li, Peter 129<br />
Li, Zheng 139<br />
Liacos, Jim 28, 38, 152, 221<br />
Liao, Jinfang 38, 223<br />
Liconic Instruments 269<br />
Lilleberg, Stan 24, 121<br />
Lillehaug, Dag 29, 161<br />
Lima, Eduardo 74<br />
309<br />
Limbach, Patrick A. 31, 175<br />
Lin, Bo 129<br />
Lin, Zhili 20, 39, 113, 224<br />
Lindsey, Jonathan S. 28, 158<br />
Liu, Ruiwu 46<br />
Lizardi, Paul 82<br />
Locascio, Laurie 20, 79<br />
Lock, Chris 108<br />
Loken, Michael R. 133<br />
Lomax, Paul 40, 234<br />
Long, Tracey 98<br />
Loo, Joseph 23, 93<br />
Loo, Rachel R. Ogorzalek 93<br />
Lopez, Mary F. 22, 36, 90, 204<br />
Los Alamos National Laboratory 269<br />
Lowe, Christopher R. 12, 16, 44<br />
Löwe, Holger 35, 203<br />
Lubeley, Mike 32, 186<br />
Luminex Corporation 271<br />
Lund, Kurt 36, 206<br />
Lunte, Susan M. 76<br />
Luttrell, Deirdre 68<br />
Ly, Jenny 39, 226<br />
M<br />
Mabuchi, Masaharu 34, 192<br />
Machamer, Joseph 31, 36, 174, 206<br />
Macherey-Nagel 271<br />
Madrigal-Gonzalez, Blanca 44<br />
Mahant, Vijay 99<br />
Maher, Julio 27, 152<br />
Mair, Dieudonne 31, 174<br />
Majumdar, A. 68<br />
Mäkinen, Maija-Liisa 38, 220<br />
Malone, Kirk 31, 175<br />
Manchester, Marianne 299<br />
Mancini, Michael A. 125<br />
Mandagere, Arun 23, 55<br />
Manger, Ingrid 29, 161<br />
Mann, Chris 37, 125, 216<br />
Manz, Andreas 24<br />
Marik, Jan 16, 46<br />
Markin, Rodney 303<br />
Marko-Varga, György 80<br />
Marrero, Jorge 82<br />
Marsh, Curtis 86<br />
Marshall, Alex 44<br />
Martel, Christine 73<br />
Martin, Donald S. 38, 80, 218<br />
Martin, Kelly T. 32, 184<br />
Martin, R. Scott 20, 76, 300<br />
Marziali, Andre 98<br />
Mast, Luke 27, 148<br />
INDEX
Masui, Colin 36, 207<br />
MATECH 271<br />
Mathes, Chris 17, 59<br />
Mathew, Anu 33, 186<br />
Mathies, Richard A. 16, 24, 44<br />
Mathrubutham, Mahesh 36, 207<br />
Matlick, Renee 33, 190<br />
MatriCal, Inc. 271<br />
Matrix Technologies Corporation 271<br />
Matus, Kim 35, 199<br />
Matuszak, Ken 23, 55<br />
McCauley, Timothy 36, 207<br />
McComb, Joel 85<br />
McCombs, Debbie 66<br />
McCoy, Mike 36, 209<br />
McDowall, Robert D. 301<br />
McGown, Evelyn 38, 223<br />
McGrath, Sara 95<br />
McIntosh, Roger 21, 77, 102<br />
McIntyre, Douglas 29, 160<br />
McKie, Jennifer 28, 34, 156, 194<br />
McRavey, Colin 135<br />
Mcree, Duncan 18<br />
MDL Information Systems 243, 272<br />
MeCour Temperature Control 272<br />
Mecomber, Justin 31, 175<br />
Mehto, Merja 36, 205<br />
Meinhart, Carl 17, 69<br />
Merel, Patrick 20, 302<br />
Merlin BioProducts BV 272<br />
Mettler-Toledo Autochem 272<br />
Metzker, Michael L. 27, 148<br />
Meutermans, Wim 16, 46<br />
Miao, Yunan 81<br />
Mickley, Mandel W. 85<br />
Micralyne, Inc. 272<br />
MicroGroup 273<br />
Micronic Systems 273, 276<br />
Micropump, Inc. 273<br />
Microscan Systems, Inc. 273<br />
Mihm, Gerhard 37, 215<br />
Mikesell, Bradley 21, 134<br />
Mikic, Ivana 125<br />
Mikkelsen, Jim 79<br />
Mikkelsen, Per Jensen 129<br />
Mikulskis, Alvydas 90<br />
Miller, Derek 141<br />
Miller, Krys J. 33, 40, 189, 233<br />
Millipore Corporation 239, 273<br />
Millis, Sherri Z. 36, 207<br />
Mineyev, Irina 135<br />
Mixon, Mark 84<br />
MJ Research, Inc. 273<br />
Modern Drug Discovery/Chemical &<br />
Engineering News 274<br />
Mogensen, Klaus B. 76<br />
Molecular BioProducts 274<br />
Molecular Devices Corporation 274<br />
Monahan, William 27, 149<br />
Montero, Felix 88<br />
Moore, Thomas 37, 215<br />
Mor, Gopal 31, 178<br />
Moran, Tim 125<br />
Morgan, Aric G. 36, 207<br />
Morganelli, Lee A. 135<br />
Morikis, Dimitrios 30, 172<br />
Mossman, Donald 21, 134<br />
Motoman, Inc. 274<br />
Motz, Gwendolyn M. 31, 176<br />
Muck, Alexander 28, 154<br />
Muckenhirn, R. 23, 29, 107, 159<br />
Mulder, Patty 32, 180<br />
Multi-Contact USA 274<br />
Munroe, David 32, 37, 184, 214<br />
Muntianu, Alexandrina 28, 84, 155<br />
Murphy, Colleen 127<br />
Murphy, Nancy 127<br />
Murray, Carl 17<br />
Murray, David 28, 152<br />
Mutz, Mitchell 63<br />
MWG Biotech, Inc. 275<br />
Myers, Ruth H. 36, 208<br />
Myslik, James 27, 149<br />
N<br />
Nagy, Kalman 27, 146<br />
Nagy, Lisa 66<br />
Najmabadi, Peyman 31, 176<br />
Nakagiri, Brent T. 28, 154<br />
Nakhai, Bita 78<br />
Namsaraev, Eugeni 101<br />
Nanostream 275, Back Cover<br />
National Instruments 275<br />
Naylor, Edwin W. 39, 224<br />
NB Corp of America 275<br />
Neeley, William 21, 115<br />
Neil, William 300<br />
Nelson, Richard M. 142, 298<br />
Nelson, Zachary 299<br />
Nettesheim, Paul 56<br />
New England Small Tube<br />
Corporation 275<br />
Newcomb, Mary 303<br />
Newman, Janet M. 85<br />
Neyer, David 48<br />
Nguyen, Jeannie 27, 37, 151, 211<br />
310<br />
Nichols, James 18, 110<br />
Nicklin, Paul 38, 126, 223<br />
Niederlaender, Harm 32, 180<br />
Niederreiter, Mateja 37, 213<br />
Nikolajsen, Rikke P. H. 76<br />
Nilsson, Johan 24, 80, 300<br />
Nirschl, David 21<br />
Nissum, Mikkel 34, 196<br />
Nordstrom, Greg 103<br />
Northrup, M. Allen 78<br />
NSK Precision America, Inc. 277<br />
NuGenesis Technologies 277<br />
NUNC Brand Products 277<br />
Nundkumar, Neelesh 39, 229<br />
Nunn, Gary 23<br />
Nutzhorn, Christa 17, 60<br />
O<br />
Oberoi, Pankaj 36, 208<br />
O’Connell, Jonathan 67<br />
Okinaka, Richard T. 100<br />
Okwuonu, Geoffrey 27, 148<br />
Olah, Marius 53<br />
Oliver, J. C. 83<br />
Ollikainen, Olavi 62<br />
Olofsson, Jessica 73<br />
Olson, Gwyneth 28, 155<br />
Olson, Jeff 36, 209<br />
Olson, Sheri 23, 106<br />
Onofrey, Tom 36, 206<br />
Ooms, Bert 27, 34, 148, 192<br />
Oprea, Tudor 21, 53<br />
Opticon, Inc. 277<br />
Organ, Andrew 18<br />
Oriental Motor USA Corp. 277<br />
Orochem Technologies, Inc. 278<br />
Oroskar, Asha 33, 190<br />
Orwar, Owe 73<br />
Osechinskiy, Sergey 39, 227<br />
Osmanagic, Emir 21, 133<br />
Ottl, Johannes 65<br />
Ouyang, Zheng 17, 83<br />
Oyster Bay Pump Works, Inc. 278<br />
Ozkan, Cengiz S. 20, 32, 36, 75,<br />
179, 209<br />
Ozkan, Mihrimah 30, 177, 179<br />
P<br />
Padmanabha, Ramesh 18, 61<br />
Pajak, Laura 31, 36, 37, 40, 99,<br />
177, 210, 212, 234<br />
Pall Life Sciences 278
Palma, John 23<br />
Pan, Jeff 36, 209<br />
Pande, Rajiv 39, 227<br />
Pantoliano, Michael 33, 73, 188<br />
Papen, Roeland 63<br />
Papp, Audrey 24, 119<br />
Pappaert, Kris 70<br />
Paramban, Rosanto 135<br />
Pardington, Paige E. 100<br />
Park, Charles 33, 188<br />
Park, Sabrina 135<br />
Parker, Geoff 36, 210<br />
Parker Hannifin Corporation 278<br />
Parsons, Thomas 73<br />
Partanen, Maija 36, 205<br />
Patel, Paren 19, 33, 39, 63, 187, 227<br />
Patel, Riddhi 28, 154<br />
Patrie, Steven 92<br />
Paul, Philip 48<br />
Paules, Richard S. 56<br />
Payne, Fiona 36, 211<br />
PerkinElmer Life and Analytical<br />
Sciences 278, 279<br />
Petersen, Jim 135<br />
Petersen, Jonathan 27, 37, 151, 211<br />
PharmaGenomics Magazine 280<br />
Phiengsai, Ma 63<br />
Phillips, Chris 33, 187<br />
Pierce Biotechnology, Inc. 280<br />
Piggott, Carolyn 39, 230<br />
Pihl, Johan 73<br />
Pilarski, L. M. 71<br />
Pittman, Chad 31, 36, 37, 40, 88,<br />
99, 177, 210, 212, 234<br />
Place, Ileana 37, 212<br />
Pneutronics Division of Parker<br />
Hannifin Corporation 280<br />
Poetter, Thorsten 37, 215<br />
Poetz, Dominik 31, 178<br />
Point Technologies, Inc. 271<br />
Pole, David 37, 217<br />
Pollard, Martin 35, 198<br />
Pollard, Mike 19, 49<br />
Pombo-Villar, Esteban 23, 57<br />
Popat, Ketul C. 31, 178<br />
Popp, Dieter 37, 213<br />
Popper & Sons, Inc. 281<br />
Porvair Sciences Ltd. 281<br />
Posch, Johannes 31, 37, 179, 213<br />
Pourahmadi, Farzad 78<br />
Prasad, Shalini 32, 179<br />
Predki, Paul 17, 82<br />
Price, Jeffrey 19, 125<br />
Prince, Amie 92<br />
Pritchard, Fred 23, 57<br />
Probst, Gerald 31, 37, 179, 213<br />
Process Analysis and Aut<strong>omation</strong> 281<br />
Proefke, Mark 39, 229<br />
Profit, Rachael 38, 126, 223<br />
Promega Corporation 281<br />
Protedyne Corporation 281<br />
Q<br />
Quarles, Jason 29, 160<br />
Quenzer, Terri 134<br />
R<br />
Rad, Hosssein Fakhrai 101<br />
Raine, Paul 37, 216<br />
Rakestraw, Dave 18, 48<br />
Rank, David 77<br />
Rapiejko, Peter 34, 192<br />
RAPP POLYERE GmbH 282<br />
Rasmussen, Lynn 37, 214<br />
Razvi, Enal 19, 87<br />
Reardon, Holly 39, 232<br />
Reed, Kirby 32, 37, 180, 214<br />
Reeves, Scott 37, 215<br />
Rehm, Jason 48<br />
REMP AG 282<br />
Renard, Simon 37, 215<br />
Renger, Thomas 36, 205<br />
ReTiSoft, Inc. 282<br />
Reynes, Julie A. 86<br />
Rheodyne LLC 282<br />
Rhode, Heidrun 37, 215<br />
Rhodes, John D. 12, 16, 43<br />
Richard Scientific, Inc./ Sepiatec<br />
GmbH 282<br />
Richmond, Steve 19, 37, 125, 216<br />
Richter, Hans-Thomas 28, 155<br />
Rieux, Laurent 32, 180<br />
Rigaku 282<br />
Riling, Dave 21, 103<br />
Ringeling, Peter 27, 34, 148, 192<br />
Rivrud, Tommy 29, 34, 161, 195<br />
Rixan Associates 283<br />
Roberts, Simon 23, 105<br />
Robinson, Dana 92<br />
RoboDesign International, Inc. 283<br />
Roby, Keith 19, 32, 38, 99, 185, 224<br />
Roche Applied Science 283<br />
Roche Instrument Center Ltd. 283<br />
Rodrigues, George 141<br />
Roenneburg, Luke 37, 216<br />
Rogers, Alex L. 141<br />
311<br />
Ronaghi, Mostafa 101<br />
Rönnmark, Sanna 38, 220<br />
Roque-Biewer, Maria 97<br />
Ross, Alfred 47<br />
Ross, Kate 32,181<br />
Roth, Michael 92<br />
Rouzer, Melissa 88<br />
Roy, Randall 18, 110<br />
RSF Electronics, Inc. 283<br />
RTS Life Science International 283<br />
Rubin, Erik 24, 300<br />
Rubin, Kara 24, 94<br />
Rudlof, Stephan 32, 181<br />
Ruff, David 97<br />
Rule, Geoffrey 92<br />
Rulison, Aaron 69<br />
Rupp, Bernhard 85<br />
Rusch, Terry L. 104<br />
Russo, Mark F. 299<br />
S<br />
Sadée, Wolfgang 119<br />
SAGE Publications 284<br />
Sakthivel, P. 32, 184<br />
Salimi-Moosavi, Hossein 89<br />
Salisbury, Cleo M. 91<br />
Salka, Leana 111<br />
Salvatore, Michael J. 38, 221<br />
Sanghera, Jas 37, 217<br />
Santiago, Juan G. 17, 19, 70, 72,<br />
132<br />
Santos, Laura 138<br />
Sanyo Scientific 284<br />
Sapp, Lisa 36, 204<br />
Sartain, Felicity 44<br />
Sauder, Michael 86<br />
Saulenas, John 21, 114<br />
Sawyer, Jaymie 21, 135<br />
Saxinger, Carl 37, 214<br />
Scammell, Russell 18, 47<br />
Schaefer, Burkhard 32, 182, 298<br />
Schäfer, Reinhold 17, 122<br />
Schaefer, Timothy 33, 186<br />
Schlotterbeck, Götz 18, 47<br />
Schmidt, Eric 38, 217<br />
Schmidt, Peter M. 88<br />
Schneemann, Anette 299<br />
Schonning, Michael 28, 154<br />
Schoot, Bart van der 30, 31, 167, 173<br />
Schotanus, Mark 82<br />
Schroen, Dan 30, 166<br />
Schübel, Ullrich 40, 234<br />
Schultz, Gary 23, 92<br />
INDEX
Schultz, Henry 24, 142<br />
Schulz, Stephen 129<br />
Schulze, M. 37, 215<br />
Schumacher, Richard T. 78<br />
Schwalbe, Thomas 19, 50<br />
Schwartz, Donald 24, 38, 80, 218<br />
Schwarzkopf, Kevin 104<br />
Schwinn, Ken 86<br />
SCIENCE/AAAS 284<br />
Scientific Specialties, Inc. 284<br />
Scinomix, Inc. 284<br />
Scivex 284<br />
Seeley, Kevin 34, 35, 193, 201<br />
Seet, Henrietta 33, 189<br />
Segelke, Brent 18, 85<br />
Seidelmann, Joachim 140<br />
Seidenman, Pam 137<br />
Seligmann, Bruce 21, 132<br />
Semin, David 21, 132, 135<br />
Senn, Hans 47<br />
Serrano, Leo 21, 114<br />
Seyfert-Margolis, Vicky 97<br />
Seyonic SA 285<br />
SGE, Inc. 285<br />
Shannon, Mark 17, 97<br />
Shapiro, John 32, 182<br />
Sharma, Sadhana 32, 182<br />
Shaw, Gerri 106<br />
Shaw, Jim 106<br />
Sheridan, Steven D. 35, 38, 201, 219<br />
Shi, Yining 89<br />
Shih, Jason 81<br />
Shiina, Toshiyuki 24, 141<br />
Shimadzu Biotech 285<br />
Shumate, Chris 124<br />
Siano, Michael A 29, 34, 39, 162,<br />
196, 231<br />
Sias 285<br />
Sidler, Rick 24, 95<br />
Sigal, George 35, 202<br />
Siguenza, Patricia Y. 28, 154<br />
Silbergleit, Arkadiy 120<br />
Silex Microsystems AB 286<br />
Silicon Valley Scientific, Inc. 285<br />
Silva, Christopher 38, 219<br />
Simonian, Michael H. 32, 38, 185,<br />
220, 224<br />
Sinclair, Jon 73<br />
Singh, Onkar 103<br />
Singh, Sharat 89<br />
Sinville, Rondedrick 72<br />
Sippola, Katja 38, 220<br />
Siu, Amy 22, 28, 38, 68, 152, 221<br />
Siuzdak, Gary 299, 302<br />
SKF USA Inc. 286<br />
Skwish, Stephen 38, 221<br />
SLR Systems 286<br />
SMAC 286<br />
Smallmon, Terrence 21, 30, 101,<br />
169<br />
Smith, Ginger 28, 38, 152, 221<br />
Smith, Janet 34, 192<br />
Smith, Malcolm 33, 186<br />
Smith, Thomas 20, 51<br />
Sneader, Walter 23, 58<br />
Song, Aimin 46<br />
Sooter, Letha 32, 183<br />
Soper, Steven A. 18, 22, 72<br />
Sørensen, Henrik Schiøtt 32, 183<br />
Sorenson BioScience 286, 287<br />
Spaid, Michael 19, 79<br />
Spark Holland, Inc. 288<br />
Sparton Medical Solutions 288<br />
Spence, Dana M. 76<br />
SPEX CertiPrep 288<br />
Spieles, Gisbert 35, 202<br />
Spillman, Scott 28, 154<br />
Sportsman, Richard 27, 151<br />
Sridharan, Duraisamy 32, 184<br />
Sridharan, Gautham 28, 154<br />
Srivatsa, S. K. 32, 184<br />
Staab, Torsten A. 141, 298<br />
Stachowiak, Timothy 31, 174<br />
Stanaitis, Michael L. 36, 207<br />
Stangegaard, Michael 129<br />
Stanley, Robert A. 123<br />
Stappert, Jorg 19, 62<br />
Staubli Corporation 288<br />
Stearns, Richard 63<br />
Stephen, Sarah 37, 125, 216<br />
Stephens, Jared 30, 172<br />
Stephens, Kathryn 89<br />
Sterling, Craig 84<br />
Stett, Alfred 60<br />
Stevens, Richard 35, 199<br />
Stevenson, Jay 27, 149<br />
Stevenson, Robert L. 17, 123<br />
Stewart, Claudia 32, 184<br />
Stewart, David 36, 208<br />
Stewart, Frances 65<br />
Stewart, Lance 18, 28, 84, 155<br />
Stiller, Hans-Joachim 38, 222<br />
Stilz, Hans-Ulrich 39, 228<br />
Stock, David 67<br />
Stoll, Norbert 24, 32, 38, 93, 185,<br />
222<br />
312<br />
Stoll, Regina 31, 32, 36, 38, 173,<br />
185, 205, 222<br />
Stoops, Brad 66<br />
Strachar, Michelle L. 69<br />
Stringer, Rowan 19, 38, 126, 223<br />
Strom, Christian 27, 149<br />
Strulovici, Berta 37, 215<br />
Su, Andrew 118<br />
Su, Michael 38, 223<br />
Suarez, Miryam Fernandez 19, 49<br />
Suen, Yu 32, 38, 185, 224<br />
Suontausta, Jari 38, 220<br />
Sutton, Shelby 72<br />
Suzow, Joseph G. 39, 224<br />
Svec, Frantisek 31, 174<br />
Swanson, Barbara 28, 153<br />
Swartz, Ken 69<br />
Tai, Yu-Chong 81<br />
Takats, Z. 83<br />
Tallarico, John 53<br />
Tan, Yuping 89<br />
T<br />
Tangkilisan, Anny 39, 225<br />
Tao, Feng 78<br />
Tao, Sarah 32, 186<br />
Tarlov, Michael J. 64<br />
Taylor, Paul 21, 24, 142<br />
Tecan/Tecan Systems Inc. 240, 243,<br />
289, 290<br />
Technical Manufacturing<br />
Corporation 290<br />
Teixeira, Gayle 27, 37, 151, 211<br />
TekCel, Inc. 241, 244, 290<br />
TEKnova 290<br />
Telechem Int/Arrayit.com 290<br />
Tennant, Raymond W. 56<br />
Tessier, Stephen 36, 208<br />
The Aut<strong>omation</strong> Partnership 238, 291<br />
The Lee Company 291<br />
Thermo Electron 241, 244, 291<br />
THK America 291<br />
Thomas, Gloria 72<br />
Thorbjornsen, Tine 127<br />
Threadgill, Graham 32, 38, 88, 185,<br />
220<br />
Thurow, Kerstin 33, 38, 39, 93,<br />
191, 222, 225<br />
Tian, Tina 89<br />
Tiemann, David 35, 203<br />
Timpone, Joe 34, 195<br />
Tischler, Jeff 87<br />
Titertek Instruments 291
Titian Software Ltd. 291<br />
Tom, Warren 106<br />
Tomtec 292<br />
Toppani, Dominique 85<br />
Torcon Instruments, Inc. 292<br />
TradeWinds Direct, Inc. 292<br />
Tran, Diem 36, 205<br />
Tricontinent 292<br />
Trout, Melissa 39, 226<br />
Tseng, Ben 19, 126<br />
Tsipouras, Athanasios 53<br />
Tsu, Christopher 19, 33, 73, 188<br />
TTP LabTech 292<br />
Turecek, Frantisek 20, 52<br />
Turino, Cynthia 127<br />
Turner, Nicholas J. 31, 175<br />
Turpin, Pierre 28, 155<br />
Tuzmen, Pinar 78<br />
U<br />
Umek, Robert 33, 39, 186, 226<br />
Union Biometrica, Inc. 292<br />
United Chemical<br />
Technologies, Inc. 293<br />
Unver, Hakki 20, 131<br />
USA Scientific, Inc. 293<br />
Usmani, Kamran 27, 148<br />
V<br />
V&P Scientific, Inc, 293<br />
Vairavan, Ram 18, 99<br />
Vajjhala, Surekha 33, 39, 187, 227<br />
Vakhshoori, Daryoosh 38, 217<br />
Van Arsdell, Scott 39, 227<br />
Van Pelt, Colleen K. 81, 92<br />
Vanderhoeven, Johan 18, 70<br />
Vankrunkelsven, Sarah 70<br />
Vaudin, Mark 33, 190<br />
Vasserot, Alain P. 28, 153<br />
Veeco Instruments Inc. 293<br />
Velasco, Jennifer 78<br />
Velasquez, David 21, 115<br />
Velocity11 293<br />
Venit, Jack 94<br />
Ventura, Manuel 134<br />
Verner, Andrei 21, 102<br />
Verpoorte, Elisabeth 32, 180<br />
VICI Valco Instruments 293<br />
Vielmetter, Jost 19, 87<br />
Vijayendren, Ravi 79<br />
Vikstrom, Sofia 39, 228<br />
Vilbrandt, Reinhard 31, 173<br />
Vogel, John S. 30, 170<br />
Von Delft, Frank 18, 84<br />
von Nickisch-Rosenegk, Markus 105<br />
von Roedern, Erich 39, 228<br />
Vreeland, Wyatt 79<br />
Vy-Trinh, Thi Ngoc 39, 232<br />
W<br />
Wachsman, William 23<br />
Wagner, Peter 17, 83<br />
Walker, John 23, 118<br />
Walkinshaw, Malocolm 31, 175<br />
Wallace, John 29,163<br />
Wallman, Lars 80<br />
Waluszko, Alex 29, 163<br />
Wang, Kena 91<br />
Wang, Joseph 28, 154<br />
Wang, Wei 39, 229<br />
Wang, Xiaobing 46<br />
Warrington, Brian H. 49<br />
Wartenberg, Niels 298<br />
Waters Corporation 294<br />
Watson-Marlow Bredel Pumps 294<br />
Weber, Angelika 39, 228<br />
Weber, James L. 104<br />
Weeks, Terry 30, 171<br />
Weimer, Wayne 20, 75<br />
Weiss, Alan 27, 147<br />
Wellin, Paul 302<br />
Wendel, Gregory 131<br />
Wetherell, John 302<br />
Whatman 294<br />
Wheeler, Mike 39, 230<br />
White Carbon 294<br />
Whitehall, Ian 33, 39, 187, 230<br />
Wikswo, John 74<br />
Wilcox, Sheri 21<br />
Wildey, Mary Jo 131<br />
Willis, Chris 39, 231<br />
Willis, Roy C. 29, 34, 162, 196<br />
Willis, Tom 19, 101<br />
Willmott, Elizabeth 38, 223<br />
Wilson, Dave 29, 160<br />
Wilson, Steven 39, 231<br />
Winick, Jeffrey 120<br />
Winner, Florian 62<br />
Winoto, Adrian 23, 79<br />
Wiseman, J. M. 83<br />
Wittel, Andrew 124<br />
Wogan, Lewis T. F. 106<br />
313<br />
Wohlstadter, Jacob N. 33, 35, 36,<br />
39, 186, 202, 208, 226<br />
Wong, Lester S. Y. 40, 232<br />
Wong, Stephanie Y. F. 49<br />
Wong, Steven H. 121<br />
Worley, Jennings 17, 58<br />
Wu, Hayley 33, 39, 188, 232<br />
Wu, Hongkai 33, 188<br />
Wu, John T. Y. 40, 232<br />
Wu, Qi-Long 35, 203<br />
Wylie, Paul 33, 39, 187, 230<br />
X<br />
Xiao, Ming 19, 100<br />
Xie, Jun 81<br />
Xu, Jia 17, 59<br />
Xu, Weiwei 34, 196<br />
Y<br />
Yamashiro, Carl 24, 120<br />
Yang, Mo 32, 179<br />
Yang, Qinghong 33, 189<br />
Yang, Wendy 33, 189<br />
Yang, Xiao-Ping 44<br />
Yavilevich, Michael 40, 233<br />
Yegneswaran, Subramanian 89<br />
Yesionek, Gary 33, 189<br />
Yingling, Jeff 142<br />
Yingyongnarongkul, Boon-ek 28, 157<br />
Yost, David 37, 213<br />
Yu, Adong 104<br />
Yurkovetsky, Yevgeny 69<br />
Zangmeister, Rebecca 19, 64<br />
Z<br />
Zare, Richard N. 33, 188<br />
Zhang, Hui 29, 163<br />
Zhang, Ji-Hu 20<br />
Zhang, Jie 118<br />
Zhang, Li 33, 39, 187, 227<br />
Zhang, Lilly 33, 40, 189, 227, 233<br />
Zhang, Ruth 40, 234<br />
Zhang, Sheng 81, 92<br />
Zhang, Xuan 32, 179<br />
Zhang, Yan 28, 34, 156, 194<br />
Zhellohang, Wei-Wei 22, 91<br />
Zhou, Heping 82<br />
Zhu, Li 72<br />
Zhu, Shirley 97<br />
Ziegert, Tillmann 36, 204<br />
INDEX
Zimmermann, Juergen 40, 234<br />
Zimmermann, Peter 37, 215<br />
Zinn, Thomas 40, 234<br />
Zinsser Analytic GmbH 294<br />
Zinsser, Werner 36, 211<br />
Zosel, Andrew 298<br />
Zymark Corporation 294<br />
314
NOTES<br />
315
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