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2015SupplementFULLTEXT
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HEPATOLOGY, VOLUME 62, NUMBER 1 (SUPPL) AASLD ABSTRACTS 1309A<br />
Day 7 from pre-dose through 48 hrs post-dose. Results. Plasma<br />
IFNa was significantly increased from baseline in R vs.NR<br />
(Fig 1; P
1308A AASLD ABSTRACTS HEPATOLOGY, October, 2015 EDP-239 plasma PK parameters following oral dosing in HCV patients Disclosures: Lijuan Jiang - Employment: Enanta Pharmaceuticals, Inc Xuemin Jiang - Employment: Novartis Eric Lawitz - Advisory Committees or Review Panels: AbbVie, Achillion Pharmaceuticals, Regulus, Theravance, Enanta, Idenix Pharmaceuticals, Janssen, Merck & Co, Novartis, Gilead; Grant/Research Support: AbbVie, Achillion Pharmaceuticals, Boehringer Ingelheim, Bristol-Myers Squibb, Gilead Sciences, GlaxoSmith- Kline, Idenix Pharmaceuticals, Intercept Pharmaceuticals, Janssen, Merck & Co, Novartis, Nitto Denko, Theravance, Salix, Enanta; Speaking and Teaching: Gilead, Janssen, AbbVie, Bristol Meyers Squibb Fred Poordad - Advisory Committees or Review Panels: Abbott/Abbvie, Achillion, BMS, Inhibitex, Boeheringer Ingelheim, Pfizer, Genentech, Idenix, Gilead, Merck, Vertex, Salix, Janssen, Novartis; Grant/Research Support: Abbvie, Anadys, Achillion, BMS, Boehringer Ingelheim, Genentech, Idenix, Gilead, Merck, Pharmassett, Vertex, Salix, Tibotec/Janssen, Novartis Stefan Zeuzem - Consulting: Abbvie, Bristol-Myers Squibb Co., Gilead, Merck & Co., Janssen Kenneth Tack - Consulting: Enanta Pharmaceuticals, Inc Richard Colvin - Employment: Novartis Institute for Biomedical Research Yat Sun Or - Employment: Enanta Pharmaceuticals, Inc, Enanta Pharmaceuticals, Inc The following authors have nothing to disclose: Kiran Dole, Kimberley Caliri 2261 Short-term safety and pharmacokinetics of co-administration of EDP239, an HCV NS5A inhibitor, and alisporivir (ALV), a cyclophilin inhibitor, in healthy subjects Xuemin Jiang 1,2 , Richard Colvin 1,2 , June Ke 1,2 , Sachin Desai 1,2 , Surendra Machineni 1,2 , Jie Zhang 1,2 , Wei Zhou 1,2 , Sun Haiying 1,2 , Steven J. Kovacs 1,2 ; 1 Novartis, East Hanover, NJ; 2 Institutes for Biomedical Research, East Hanover, NJ Introduction: Cyclophilin A plays essential roles in HCV replication and assembly, including modulating the function of NS5A. Combined cyclophilin and NS5A inhibition is a clinically untested therapeutic approach despite in vitro <strong>studies</strong> suggesting additive-to-synergistic effects of the two mechanisms, acting at functionally dependent sites of the HCV replication complex. An EDP239 C 24h of 317 ng/mL reduced viral load by >3 log10 after a single 100 mg dose in GT1-infected patients. ALV is a cyclophilin inhibitor that has pan-genotypic anti-HCV activity with demonstrated clinical efficacy. It is a time-dependent CYP3A4 inhibitor and inhibitor of multiple uptake and efflux transporters, including P-gp. EDP239 is a substrate of CYP3A4 and P-gp in vitro. Methods: Open-label; fixed sequence design (n=26). Subjects received multiple doses of EDP239 30 mg BID for 21 days with co-administration of ALV 400 mg BID days 8 through 21. Both drugs administered with food. Blood samples were collected up to 12 hours after dose administration on days 7, 15 and 21 to measure plasma concentrations by LC/ MS/MS. Non-compartmental PK analysis using WinNonlin; ANOVA for statistical comparisons; routine safety assessments with safety data descriptively summarized. Results: 22 subjects completed the study. Study treatments were well tolerated with no safety-related discontinuations. Adverse effects were consistent with the known effects of either EDP239 (gastrointestinal complaints) or ALV (gastrointestinal complaints, headache, laboratory abnormalities, increases in blood pressure). Median EDP239 T max was 3, 4.5, and 4 h on days 7, 14, and 21, respectively. The EDP239 C 12h was 2180 ng/mL when administered with ALV. EDP 100 mg was comparable to C12h of EDP 30 mg when combined with a alisporivir. EDP239 had no apparent effect on ALV exposure (compared with historical data). Conclusion Short term co-administration of EDP239 and ALV was safe and well tolerated. The data suggest that therapeutic combination of ALV and EDP239 is feasible. The observed EDP239 exposures can be expected to have clinical activity against GT1 and GT3 HCV. The time course of exposure to EDP239 was relatively flat with minimal difference between peak and trough. Exploiting the interaction may allow EDP239 to treat patients with G3 HCV infection without increasing overall exposure beyond exposures previously safely investigated in humans. Disclosures: Xuemin Jiang - Employment: Novartis Richard Colvin - Employment: Novartis Institute for Biomedical Research Surendra Machineni - Employment: Novartis Healthcare Private Limited Steven J. Kovacs - Employment: Novartis The following authors have nothing to disclose: June Ke, Sachin Desai, Jie Zhang, Wei Zhou, Sun Haiying 2262 SB 9200 Shows Antiviral Activity Against HCV-RNA by Targeting Host Cytosolic Sensor Proteins RIG-I and NOD-2 to activate IFN signaling pathways Kumar Visvanathan 2,1 , Rosemary M. Millen 2,1 , Stuart K. Roberts 4,5 , Peter W. Angus 6,2 , Wendy Cheng 8 , Nada Farhat 9 , My My Trinh 9 , Radhakrishnan P. Iyer 10 , Murray L. Barclay 7 , Alex J. Thompson 3,2 ; 1 Innate Immunity Laboratory, St Vincent’s Hospital Melbourne, Melbourne, VIC, Australia; 2 Medicine, Univeristy of Melbourne, Melbourne, VIC, Australia; 3 Gastroenterology, St. Vincents Hospital, Melbourne, Fitzroy, VIC, Australia; 4 Gastroenterology, Alfred Hospital, Melbourne, VIC, Australia; 5 Medicine, Monash University, Melbourne, VIC, Australia; 6 Liver Transplant Unit, Austin Hospital, Melbourne, VIC, Australia; 7 Gastroenterology & Clinical Pharmacology, Christchurch Hospital and University of Otago, Christchurch, New Zealand; 8 Gastroenterology, Royal Perth Hospital, Perth, WA, Australia; 9 Pharsight Consulting Services, Princeton, NJ; 10 Spring Bank Pharmaceuticals, Milford, MA Introduction. SB9200 activates RIG-I and NOD-2 pathways in virally infected cells and has been shown to maximally reduce HCV RNA by 1.9 log10 in HCV patients (Thompson EASL 2015). We aimed to evaluate the relationship between the pharmacokinetics of SB 9200, and the induction of innate immunity and antiviral response. Methods. HCV patients, GT 1 and 3, received 200-900 mg of SB9200 PO daily for 7 days. Early antiviral kinetics were measured from baseline through 7 days after the first dose of SB 9200. Patients were divided into null responders at day 7 (NR, < 0.5 log10 reduction in HCV RNA, n=11) or responders (R, >0.9 log10, n=6). Clinical characteristics at baseline for NR vs. R were similar for median HCV RNA, BMI, ALT, AST, platelets and Fibroscan. 50% of R were IL28b CC compared to 18% of NR. Plasma interferon alpha (IFNa) was measured on day 1, 7 and 14 by ELISA. Interferon stimulating genes ISG15 and OAS1 were measured on Day 1, 2, 3, 7 and 14 in peripheral blood via RT-PCR. GAPDH was used as an internal control. Data are represented as ratios from baseline for IFNa, ISG15, and OAS1. For pharmacokinetic analysis, blood samples were collected on
HEPATOLOGY, VOLUME 62, NUMBER 1 (SUPPL) AASLD ABSTRACTS 1309A Day 7 from pre-dose through 48 hrs post-dose. Results. Plasma IFNa was significantly increased from baseline in R vs.NR (Fig 1; P
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