INCIDENCE OF VIRUS INFECTIONS ON DIFFERENT PEACH CULTIVARS IN MONTENEGRO
incidence of virus infections on different peach cultivars in ... - Izbis incidence of virus infections on different peach cultivars in ... - Izbis
• Real Time RT-PCR identified more PPV infected samples than RT-PCR indicating higher sensitivity • Amplification of different amplicons from the detected viruses by RT-PCR, followed by cloning of complete and partial sequences of CP gene allowed phylogenetic analysis of isolates of different detected viruses: PPV (8), PNRSV (4) and PDV (1) • Sequence analyses revealed that all Montenegrin PPV isolates shared from 92.2 to 99.1% nucleotide identity with corresponding PPV-M strain sequences deposited in GenBank
• Results indicate the most probable introduction of PPV in Montenegro through peach propagation material which is mainly imported from Greece where this strain is the most prevailing one • Phylogenetic analysis suggesting two possible introductions of PNRSV in Montenegro • Molecular grouping of PNRSV isolates wasn’t in correlation with geographical region and host • Urgent sanitation measures should be taken: eradication of infected trees severe control related to importation of plant material
- Page 1 and 2: INCIDENCE OF VIRUS INFECTIONS ON DI
- Page 3 and 4: Investigation on peach viruses Prev
- Page 5 and 6: Examined cultivars and viruses Cul
- Page 7 and 8: Field survey cv. Ritastar cv. Ritas
- Page 10 and 11: Molecular methods used for the dete
- Page 12 and 13: Incidence of PPV, PNRSV and PDV in
- Page 14 and 15: Purification of PCR products Clonin
- Page 16 and 17: Phylogenetic analysis • Phylogene
- Page 18 and 19: Molecular analysis for PNRSV • De
- Page 20 and 21: • Sequence analyses of CP gene fr
- Page 22 and 23: Molecular analysis for PDV • RT-P
- Page 26: Thank you for your attention!!!
• Real Time RT-PCR identified more PPV infected samples<br />
than RT-PCR indicating higher sensitivity<br />
• Amplification of different amplicons from the detected<br />
viruses by RT-PCR, followed by cloning of complete and<br />
partial sequences of CP gene allowed phylogenetic<br />
analysis of isolates of different detected viruses: PPV<br />
(8), PNRSV (4) and PDV (1)<br />
• Sequence analyses revealed that all Montenegrin PPV<br />
isolates shared from 92.2 to 99.1% nucleotide identity<br />
with corresponding PPV-M strain sequences deposited in<br />
GenBank
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