INCIDENCE OF VIRUS INFECTIONS ON DIFFERENT PEACH CULTIVARS IN MONTENEGRO
incidence of virus infections on different peach cultivars in ... - Izbis incidence of virus infections on different peach cultivars in ... - Izbis
Molecular analysis for PDV • RT-PCR method using PDV-2F/PDV-1R primers (173 bp) • Presence of PDV was confirmed in only 1 out of 58 samples • BLAST analysis revealed high similarity, ranging from 94.2 to 96%, with isolates reported from other parts of the world • Highest value MNE isolates showed with Ch 137 isolate (L28145) 173 bp RT-PCR for PDV (CP)
Conclusions • RT-PCR were used to identify infection by PPV, PDV, PNRSV, ACLSV, PMV, CMLV, TRSV, ToRSV and SLRSV in stone fruits. Nested-PCR (n-PCR) method was also used to detect SLRSV and Real-time RT-PCR to detect PPV • The study reported presence of one quarantine virus (PPV) and two “quality” viruses (PDV and PNRSV) • The study reported very high incidence of PPV (60.3%). PNRSV was detected in 18.9% and PDV in 1.7% assayed samples • All other assayed peach viruses were not detected in the collected samples
- Page 1 and 2: INCIDENCE OF VIRUS INFECTIONS ON DI
- Page 3 and 4: Investigation on peach viruses Prev
- Page 5 and 6: Examined cultivars and viruses Cul
- Page 7 and 8: Field survey cv. Ritastar cv. Ritas
- Page 10 and 11: Molecular methods used for the dete
- Page 12 and 13: Incidence of PPV, PNRSV and PDV in
- Page 14 and 15: Purification of PCR products Clonin
- Page 16 and 17: Phylogenetic analysis • Phylogene
- Page 18 and 19: Molecular analysis for PNRSV • De
- Page 20 and 21: • Sequence analyses of CP gene fr
- Page 24 and 25: • Real Time RT-PCR identified mor
- Page 26: Thank you for your attention!!!
Molecular analysis for PDV<br />
• RT-PCR method using PDV-2F/PDV-1R primers (173 bp)<br />
• Presence of PDV was confirmed in only 1 out of 58<br />
samples<br />
• BLAST analysis revealed high similarity,<br />
ranging from 94.2 to 96%,<br />
with isolates reported<br />
from other parts of the world<br />
• Highest value MNE isolates<br />
showed with Ch 137 isolate<br />
(L28145)<br />
173 bp<br />
RT-PCR for PDV (CP)
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