INCIDENCE OF VIRUS INFECTIONS ON DIFFERENT PEACH CULTIVARS IN MONTENEGRO
incidence of virus infections on different peach cultivars in ... - Izbis incidence of virus infections on different peach cultivars in ... - Izbis
• Sequence analyses of CP gene from PNRSV isolates proved to be 89.3 – 100% identical with corresponding sequences of isolates previously described • 2 PNRSV isolates from cv. Rita Star were most closely related to Chilean NctCl.augl isolate from nectarine (EF565253) • PNRSV isolates from cv.s May Crest and Spring Crest were most closely related to Italian isolate PchIt.may1 from peach cv. May Crest
• Sequences of 4 PNRSV isolates were deposited in GenBank (JX569825 - JX569828) • Isolates clustered within three groups: PE-5, PV-32 and PV- 96 • 2 MNE isolates from cv. Rita Star clustered in PE-5 group • Isolates from cv. May Crest and Spring Crest clustered in PV-96 Phylogenetic tree was constructed using minimum-evolution method with Tamura-Nei corrected nt distance and bootstrap analyses of 10000 replicates within MEGA 4.1 software
- Page 1 and 2: INCIDENCE OF VIRUS INFECTIONS ON DI
- Page 3 and 4: Investigation on peach viruses Prev
- Page 5 and 6: Examined cultivars and viruses Cul
- Page 7 and 8: Field survey cv. Ritastar cv. Ritas
- Page 10 and 11: Molecular methods used for the dete
- Page 12 and 13: Incidence of PPV, PNRSV and PDV in
- Page 14 and 15: Purification of PCR products Clonin
- Page 16 and 17: Phylogenetic analysis • Phylogene
- Page 18 and 19: Molecular analysis for PNRSV • De
- Page 22 and 23: Molecular analysis for PDV • RT-P
- Page 24 and 25: • Real Time RT-PCR identified mor
- Page 26: Thank you for your attention!!!
• Sequence analyses of CP gene from PNRSV<br />
isolates proved to be 89.3 – 100% identical with<br />
corresponding sequences of isolates previously<br />
described<br />
• 2 PNRSV isolates from cv. Rita Star were most<br />
closely related to Chilean NctCl.augl isolate from<br />
nectarine (EF565253)<br />
• PNRSV isolates from cv.s May Crest and Spring<br />
Crest were most closely related to Italian isolate<br />
PchIt.may1 from peach cv. May Crest
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