INCIDENCE OF VIRUS INFECTIONS ON DIFFERENT PEACH CULTIVARS IN MONTENEGRO

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Molecular analysis for PNRSV • Detection of PNRSV by RT- PCR using Ilar2F/Ilar1R primers 206 bp • 11 out of 58 assayed peach samples were positive on PNRSV • PNRSV was confirmed in 3 cultivars: Rita Star, May Crest and Spring Crest • Highest infection rate (66,7%) was confirmed in cv. May Crest RT-PCR using Ilar2F/Ilar 1R primers
Molecular characterization • 4 PNRSV isolates derived from cv.s Rita Star (2), May Crest (1) and Spring Crest (1) were chosen for further analysis • Complete CP gene was amplified by RT-PCR using MG1/MG2 primers (Glasa et al., 2008) • Isolates were cloned and sequenced Screening of PNRSV isolates by PCR using M13-for/M13-rev
Page 1 and 2: INCIDENCE OF VIRUS INFECTIONS ON DI
Page 3 and 4: Investigation on peach viruses Prev
Page 5 and 6: Examined cultivars and viruses Cul
Page 7 and 8: Field survey cv. Ritastar cv. Ritas
Page 10 and 11: Molecular methods used for the dete
Page 12 and 13: Incidence of PPV, PNRSV and PDV in
Page 14 and 15: Purification of PCR products Clonin
Page 16 and 17: Phylogenetic analysis • Phylogene
Page 20 and 21: • Sequence analyses of CP gene fr
Page 22 and 23: Molecular analysis for PDV • RT-P
Page 24 and 25: • Real Time RT-PCR identified mor
Page 26: Thank you for your attention!!!

INCIDENCE OF VIRUS INFECTIONS ON DIFFERENT PEACH CULTIVARS IN MONTENEGRO

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