INCIDENCE OF VIRUS INFECTIONS ON DIFFERENT PEACH CULTIVARS IN MONTENEGRO
incidence of virus infections on different peach cultivars in ... - Izbis incidence of virus infections on different peach cultivars in ... - Izbis
Purification of PCR products Cloning in pGEM-T Easy vector • 8 PPV isolates were purified, cloned and sequenced • Screening of colonies was performed by PCR using M13-for/M13-rev primers Purification of plasmid-DNA ~1500 bp Sequencing Screening of colonies
Molecular characterization • Nucleotide sequences of complete CP gene derived from Montenegrin isolates were compared with previously described PPV sequences deposited in GenBank (92,2 – 99,1%) • BLAST analysis showed: • MNE isolates from Gloria, May Crest, Adriana and Rita Star were the most closely related (97,1 – 99,1%) with isolate SK68 (M92280.1) • MNE isolates from Morsiani, Maria Marta and Spring Crest shared most identity (97,6 – 98,4%) with previously reported Montenegrin isolate Godinje 1 (HQ452396) from region of Bar • MNE isolate from cv. Spring Belle was most similar with sequence of Greek isolate N1 (FJ361234) from peach.
- Page 1 and 2: INCIDENCE OF VIRUS INFECTIONS ON DI
- Page 3 and 4: Investigation on peach viruses Prev
- Page 5 and 6: Examined cultivars and viruses Cul
- Page 7 and 8: Field survey cv. Ritastar cv. Ritas
- Page 10 and 11: Molecular methods used for the dete
- Page 12 and 13: Incidence of PPV, PNRSV and PDV in
- Page 16 and 17: Phylogenetic analysis • Phylogene
- Page 18 and 19: Molecular analysis for PNRSV • De
- Page 20 and 21: • Sequence analyses of CP gene fr
- Page 22 and 23: Molecular analysis for PDV • RT-P
- Page 24 and 25: • Real Time RT-PCR identified mor
- Page 26: Thank you for your attention!!!
Purification of PCR products<br />
Cloning in pGEM-T Easy vector<br />
• 8 PPV isolates were<br />
purified, cloned and<br />
sequenced<br />
• Screening of colonies was<br />
performed by PCR using<br />
M13-for/M13-rev primers<br />
Purification of plasmid-DNA<br />
~1500 bp<br />
Sequencing<br />
Screening of colonies
Change language