INCIDENCE OF VIRUS INFECTIONS ON DIFFERENT PEACH CULTIVARS IN MONTENEGRO

More documents

Recommendations

Info

Molecular methods used for the detection of stone fruit viruses Virus Primers Primers sequences (5’ - 3’) ACLSV CMLV PMV PNRSV PDV PPV TRSV ToRSV SLRSV ACLSRV F ACLSV R PM16AFF PM16AFR PM16AFF CML-26R Ilar 2F Ilar 1R PDV 2F PDV 1R PPV 8511-8532 F PPV 9763-9784 R P241 P316D P316M PPV-DM probe TRSVf1 TRSVr1 ToRSVf1 ToRSVr1 3DR F1 2R2 5DF GGCAACCCTGGAACAGA CAGACCCTTATTGAAGTCGAA CAAACATGGCTTTCACCTTCTGCA TCTTGCCCCACCCTTCAACAAATG CAAACATGGCTTTCACCTTCTGCA AGATCCTCTTTCCCTTCTAAAATG CCACCGAGAGGTTGGCA TTCTAGCAGGTCTTCATCGA ATGGATGGGATGGATAAAATAGT TAGTGCAGGTTAACCAAAAGGAT CGATATCTTGAAGCTTTTTACG CTCTTGCACAAGAACTATAACC CGTTTATTTGGCTTGGATGGAA GATTAACATCACCAGCGGTGTG GATTCACGTCACCAGCGGTGTG FAM-CGTCGGAACACAAGAAGAGGACACAGA- TAMRA GGAAGCTGTATAAACTCAGC GTGTTGGACAAACACGACAC GGAAGCTGTATAAACTCAGC GTCCTCATGGAACCTTTCTC AGGCTCAAGAAAACACAC GGCTGATCCTCGTGAGAC GCGTGTGGCGTCGCTAAT CCCTTGGTTACTTTTACCTCCT Size of amplicon 358 bp Reference Nemichov et al., 1995 Method RT-PCR 419 bp Delano and RT-PCR 705 bp Upton, 1999 RT-PCR 206 bp 173 bp Candresse et al., 1997 Parakh et al., 1995 RT-PCR RT-PCR 1274 bp This work RT-PCR 243 bp Olmos et al., 2005 Real Time PCR 338 bp Martin et al., RT-PCR 512 bp 2009 RT-PCR 739 bp RT-PCR 203 bp Ratti et al., 2008 Nested PCR
Incidence of different stone fruit’s viruses in Montenegrin peach 70.7% of samples were infected by at least one of ascertained viruses Single and double infections were confirmed, respectively, in 56.9% and 13.8% of the assayed plants Incidence of single and double infections not infected 29.3% duble infections 13.8% single infections 56.9% Molecular analysis revealed: Presence of PPV, PNRSV and PDV Double infection were found between PPV+PNRSV and PPV+PDV Absence of ACLSV, PMV, CMLV, SLRSV, TRSV and ToRSV from all tested samples
Page 1 and 2: INCIDENCE OF VIRUS INFECTIONS ON DI
Page 3 and 4: Investigation on peach viruses Prev
Page 5 and 6: Examined cultivars and viruses Cul
Page 7 and 8: Field survey cv. Ritastar cv. Ritas
Page 12 and 13: Incidence of PPV, PNRSV and PDV in
Page 14 and 15: Purification of PCR products Clonin
Page 16 and 17: Phylogenetic analysis • Phylogene
Page 18 and 19: Molecular analysis for PNRSV • De
Page 20 and 21: • Sequence analyses of CP gene fr
Page 22 and 23: Molecular analysis for PDV • RT-P
Page 24 and 25: • Real Time RT-PCR identified mor
Page 26: Thank you for your attention!!!

INCIDENCE OF VIRUS INFECTIONS ON DIFFERENT PEACH CULTIVARS IN MONTENEGRO

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?