INCIDENCE OF VIRUS INFECTIONS ON DIFFERENT PEACH CULTIVARS IN MONTENEGRO
incidence of virus infections on different peach cultivars in ... - Izbis incidence of virus infections on different peach cultivars in ... - Izbis
Molecular methods used for the detection of stone fruit viruses Virus Primers Primers sequences (5’ - 3’) ACLSV CMLV PMV PNRSV PDV PPV TRSV ToRSV SLRSV ACLSRV F ACLSV R PM16AFF PM16AFR PM16AFF CML-26R Ilar 2F Ilar 1R PDV 2F PDV 1R PPV 8511-8532 F PPV 9763-9784 R P241 P316D P316M PPV-DM probe TRSVf1 TRSVr1 ToRSVf1 ToRSVr1 3DR F1 2R2 5DF GGCAACCCTGGAACAGA CAGACCCTTATTGAAGTCGAA CAAACATGGCTTTCACCTTCTGCA TCTTGCCCCACCCTTCAACAAATG CAAACATGGCTTTCACCTTCTGCA AGATCCTCTTTCCCTTCTAAAATG CCACCGAGAGGTTGGCA TTCTAGCAGGTCTTCATCGA ATGGATGGGATGGATAAAATAGT TAGTGCAGGTTAACCAAAAGGAT CGATATCTTGAAGCTTTTTACG CTCTTGCACAAGAACTATAACC CGTTTATTTGGCTTGGATGGAA GATTAACATCACCAGCGGTGTG GATTCACGTCACCAGCGGTGTG FAM-CGTCGGAACACAAGAAGAGGACACAGA- TAMRA GGAAGCTGTATAAACTCAGC GTGTTGGACAAACACGACAC GGAAGCTGTATAAACTCAGC GTCCTCATGGAACCTTTCTC AGGCTCAAGAAAACACAC GGCTGATCCTCGTGAGAC GCGTGTGGCGTCGCTAAT CCCTTGGTTACTTTTACCTCCT Size of amplicon 358 bp Reference Nemichov et al., 1995 Method RT-PCR 419 bp Delano and RT-PCR 705 bp Upton, 1999 RT-PCR 206 bp 173 bp Candresse et al., 1997 Parakh et al., 1995 RT-PCR RT-PCR 1274 bp This work RT-PCR 243 bp Olmos et al., 2005 Real Time PCR 338 bp Martin et al., RT-PCR 512 bp 2009 RT-PCR 739 bp RT-PCR 203 bp Ratti et al., 2008 Nested PCR
Incidence of different stone fruit’s viruses in Montenegrin peach 70.7% of samples were infected by at least one of ascertained viruses Single and double infections were confirmed, respectively, in 56.9% and 13.8% of the assayed plants Incidence of single and double infections not infected 29.3% duble infections 13.8% single infections 56.9% Molecular analysis revealed: Presence of PPV, PNRSV and PDV Double infection were found between PPV+PNRSV and PPV+PDV Absence of ACLSV, PMV, CMLV, SLRSV, TRSV and ToRSV from all tested samples
- Page 1 and 2: INCIDENCE OF VIRUS INFECTIONS ON DI
- Page 3 and 4: Investigation on peach viruses Prev
- Page 5 and 6: Examined cultivars and viruses Cul
- Page 7 and 8: Field survey cv. Ritastar cv. Ritas
- Page 12 and 13: Incidence of PPV, PNRSV and PDV in
- Page 14 and 15: Purification of PCR products Clonin
- Page 16 and 17: Phylogenetic analysis • Phylogene
- Page 18 and 19: Molecular analysis for PNRSV • De
- Page 20 and 21: • Sequence analyses of CP gene fr
- Page 22 and 23: Molecular analysis for PDV • RT-P
- Page 24 and 25: • Real Time RT-PCR identified mor
- Page 26: Thank you for your attention!!!
Molecular methods used for the detection of stone fruit viruses<br />
Virus Primers Primers sequences (5’ - 3’)<br />
ACLSV<br />
CMLV<br />
PMV<br />
PNRSV<br />
PDV<br />
PPV<br />
TRSV<br />
ToRSV<br />
SLRSV<br />
ACLSRV F<br />
ACLSV R<br />
PM16AFF<br />
PM16AFR<br />
PM16AFF<br />
CML-26R<br />
Ilar 2F<br />
Ilar 1R<br />
PDV 2F<br />
PDV 1R<br />
PPV 8511-8532 F<br />
PPV 9763-9784 R<br />
P241<br />
P316D<br />
P316M<br />
PPV-DM probe<br />
TRSVf1<br />
TRSVr1<br />
ToRSVf1<br />
ToRSVr1<br />
3DR<br />
F1<br />
2R2<br />
5DF<br />
GGCAACCCTGGAACAGA<br />
CAGACCCTTATTGAAGTCGAA<br />
CAAACATGGCTTTCACCTTCTGCA<br />
TCTTGCCCCACCCTTCAACAAATG<br />
CAAACATGGCTTTCACCTTCTGCA<br />
AGATCCTCTTTCCCTTCTAAAATG<br />
CCACCGAGAGGTTGGCA<br />
TTCTAGCAGGTCTTCATCGA<br />
ATGGATGGGATGGATAAAATAGT<br />
TAGTGCAGGTTAACCAAAAGGAT<br />
CGATATCTTGAAGCTTTTTACG<br />
CTCTTGCACAAGAACTATAACC<br />
CGTTTATTTGGCTTGGATGGAA<br />
GATTAACATCACCAGCGGTGTG<br />
GATTCACGTCACCAGCGGTGTG<br />
FAM-CGTCGGAACACAAGAAGAGGACACAGA-<br />
TAMRA<br />
GGAAGCTGTATAAACTCAGC<br />
GTGTTGGACAAACACGACAC<br />
GGAAGCTGTATAAACTCAGC<br />
GTCCTCATGGAACCTTTCTC<br />
AGGCTCAAGAAAACACAC<br />
GGCTGATCCTCGTGAGAC<br />
GCGTGTGGCGTCGCTAAT<br />
CCCTTGGTTACTTTTACCTCCT<br />
Size of<br />
amplicon<br />
358 bp<br />
Reference<br />
Nemichov et<br />
al., 1995<br />
Method<br />
RT-PCR<br />
419 bp<br />
Delano and<br />
RT-PCR<br />
705 bp<br />
Upton, 1999<br />
RT-PCR<br />
206 bp<br />
173 bp<br />
Candresse<br />
et al., 1997<br />
Parakh et al.,<br />
1995<br />
RT-PCR<br />
RT-PCR<br />
1274 bp This work RT-PCR<br />
243 bp<br />
Olmos et al.,<br />
2005<br />
Real Time PCR<br />
338 bp<br />
Martin et al.,<br />
RT-PCR<br />
512 bp<br />
2009<br />
RT-PCR<br />
739 bp<br />
RT-PCR<br />
203 bp<br />
Ratti et al.,<br />
2008 Nested PCR
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