kvalitet
Vol.55 - Izbis Vol.55 - Izbis
92 Jelica Balaž, Aleksandra Smiljanić Fig. 6 – The spots with bacterial ooze on pear immature fruits inoculated with strains Kot I/1 and 8/2 (Orig). Pege sa bakterijskim eksudatom na nezrelim plodovima kruške inokulisanih izolatima Kot I/1 i 8/2 (Orig.). All strains performed uniform reaction to antibiotics and were sensitive to amykacin, eritromycin, neomycin, streptomycin, nitrofurantion and kanamycin and resistant to vancomycin. The diameters of inhibitione zones formed by
Chaenomeles japonica and Cotoneaster horizontalis 93 nitrofurantion, kanamycin and amykacin, and streptomycin and eritromycin were 30-35 mm and 25-30 mm, respectively. DISCUSSION Bacterial fireblight is very serious problem in Serbia. Few years after its first recording on apple, pear and quince in Serbia (Arsenijević, 1990), the causer agent (E.amylovora) was isolated from from spontaneous and ornamental plants: – Pyracantha coccinea (Gavrilovic and Arsenijevic, 1998), C. japonica and C. horisontalis (Balaž Jelica, 2000; Balaž Jelica et al., 2004). The disease occurrence on C. japonica and C. horisontalis in 2000 was favored by high temperatures during flowering period of apple in the nearby oarches. Morphological, cultural, biochemical, physiological and serological characteristics and sensitivity to antibiotics showed that our strains obtained from C. japonica and C. horizontals were identical to referent NCPPB 595 and JK strains from apple. Bacteriological characteristics of our strains were in accordance to the results of other researches who studied numerous E. amylovora strains of pomaceous fruits from different geographic area of Europe and USA. Further investigations on molecular level or by enrichment serological methods, would perhaps show some differences between strains. All our strains were sensitive to aplied antibiotics except to vancomycin. Morgan and Goodman (1955) found aureomycin, neomycin, streptomycin, polymyxin, streptotrichin, viomycin and chloromycetin to be effective to E.amylovora in laboratory conditions. Penicillin didn’t inhibite growth of E.amylovora in vitro according to Billing et al. (1961). On the other hand, Rudolph (1946) found penicillin to inhibit pathogen in vitro, but its control of Fire blight failed in the field experiments. Martinec and Kocur (1964) found that all 49 tested strains of E.amylovora were sensitive to chloramphenicol, erytromycin, neomycin, streptomycin and teracycline, while 24 were sensitive to chlorotetrcycline. Although many antibiotics inhibit growth of E.amylovora in vitro, only a few have practical value for the field application (mainly streptomycin and sporadicly oxytetracycline and kasugamycin were used).
- Page 37 and 38: Anatomsko-histološke karakteristik
- Page 39 and 40: Anatomsko-histološke karakteristik
- Page 41 and 42: Anatomsko-histološke karakteristik
- Page 43 and 44: Anatomsko-histološke karakteristik
- Page 45 and 46: Odgajivačke odlike izolata Leucost
- Page 47 and 48: Odgajivačke odlike izolata Leucost
- Page 49 and 50: Odgajivačke odlike izolata Leucost
- Page 51 and 52: Odgajivačke odlike izolata Leucost
- Page 53 and 54: Odgajivačke odlike izolata Leucost
- Page 55 and 56: Odgajivačke odlike izolata Leucost
- Page 57 and 58: Odgajivačke odlike izolata Leucost
- Page 59 and 60: Odgajivačke odlike izolata Leucost
- Page 61 and 62: Uticaj načina zaštite semena ozim
- Page 63 and 64: Uticaj načina zaštite semena ozim
- Page 65 and 66: Uticaj načina zaštite semena ozim
- Page 67 and 68: Uticaj načina zaštite semena ozim
- Page 69 and 70: Uticaj načina zaštite semena ozim
- Page 71 and 72: Razvoj mikoparazita Colletrichum gl
- Page 73 and 74: Razvoj mikoparazita Colletrichum gl
- Page 75 and 76: Razvoj mikoparazita Colletrichum gl
- Page 77 and 78: Razvoj mikoparazita Colletrichum gl
- Page 79 and 80: Razvoj mikoparazita Colletrichum gl
- Page 81 and 82: Razvoj mikoparazita Colletrichum gl
- Page 83 and 84: Chaenomeles japonica and Cotoneaste
- Page 85 and 86: Chaenomeles japonica and Cotoneaste
- Page 87: Chaenomeles japonica and Cotoneaste
- Page 91 and 92: Chaenomeles japonica and Cotoneaste
- Page 93 and 94: 97 I N M E M O R I A M Dr ANTON ZAB
Chaenomeles japonica and Cotoneaster horizontalis 93<br />
nitrofurantion, kanamycin and amykacin, and streptomycin and eritromycin were<br />
30-35 mm and 25-30 mm, respectively.<br />
DISCUSSION<br />
Bacterial fireblight is very serious problem in Serbia. Few years after its first<br />
recording on apple, pear and quince in Serbia (Arsenijević, 1990), the causer<br />
agent (E.amylovora) was isolated from from spontaneous and ornamental plants:<br />
– Pyracantha coccinea (Gavrilovic and Arsenijevic, 1998), C. japonica and C.<br />
horisontalis (Balaž Jelica, 2000; Balaž Jelica et al., 2004). The disease occurrence<br />
on C. japonica and C. horisontalis in 2000 was favored by high temperatures<br />
during flowering period of apple in the nearby oarches.<br />
Morphological, cultural, biochemical, physiological and serological<br />
characteristics and sensitivity to antibiotics showed that our strains obtained<br />
from C. japonica and C. horizontals were identical to referent NCPPB 595<br />
and JK strains from apple. Bacteriological characteristics of our strains were in<br />
accordance to the results of other researches who studied numerous E. amylovora<br />
strains of pomaceous fruits from different geographic area of Europe and USA.<br />
Further investigations on molecular level or by enrichment serological methods,<br />
would perhaps show some differences between strains.<br />
All our strains were sensitive to aplied antibiotics except to vancomycin.<br />
Morgan and Goodman (1955) found aureomycin, neomycin, streptomycin,<br />
polymyxin, streptotrichin, viomycin and chloromycetin to be effective to<br />
E.amylovora in laboratory conditions. Penicillin didn’t inhibite growth of<br />
E.amylovora in vitro according to Billing et al. (1961). On the other hand,<br />
Rudolph (1946) found penicillin to inhibit pathogen in vitro, but its control of<br />
Fire blight failed in the field experiments. Martinec and Kocur (1964) found<br />
that all 49 tested strains of E.amylovora were sensitive to chloramphenicol,<br />
erytromycin, neomycin, streptomycin and teracycline, while 24 were sensitive to<br />
chlorotetrcycline.<br />
Although many antibiotics inhibit growth of E.amylovora in vitro, only a few<br />
have practical value for the field application (mainly streptomycin and sporadicly<br />
oxytetracycline and kasugamycin were used).