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92 Jelica Balaž, Aleksandra Smiljanić Fig. 6 – The spots with bacterial ooze on pear immature fruits inoculated with strains Kot I/1 and 8/2 (Orig). Pege sa bakterijskim eksudatom na nezrelim plodovima kruške inokulisanih izolatima Kot I/1 i 8/2 (Orig.). All strains performed uniform reaction to antibiotics and were sensitive to amykacin, eritromycin, neomycin, streptomycin, nitrofurantion and kanamycin and resistant to vancomycin. The diameters of inhibitione zones formed by
Chaenomeles japonica and Cotoneaster horizontalis 93 nitrofurantion, kanamycin and amykacin, and streptomycin and eritromycin were 30-35 mm and 25-30 mm, respectively. DISCUSSION Bacterial fireblight is very serious problem in Serbia. Few years after its first recording on apple, pear and quince in Serbia (Arsenijević, 1990), the causer agent (E.amylovora) was isolated from from spontaneous and ornamental plants: – Pyracantha coccinea (Gavrilovic and Arsenijevic, 1998), C. japonica and C. horisontalis (Balaž Jelica, 2000; Balaž Jelica et al., 2004). The disease occurrence on C. japonica and C. horisontalis in 2000 was favored by high temperatures during flowering period of apple in the nearby oarches. Morphological, cultural, biochemical, physiological and serological characteristics and sensitivity to antibiotics showed that our strains obtained from C. japonica and C. horizontals were identical to referent NCPPB 595 and JK strains from apple. Bacteriological characteristics of our strains were in accordance to the results of other researches who studied numerous E. amylovora strains of pomaceous fruits from different geographic area of Europe and USA. Further investigations on molecular level or by enrichment serological methods, would perhaps show some differences between strains. All our strains were sensitive to aplied antibiotics except to vancomycin. Morgan and Goodman (1955) found aureomycin, neomycin, streptomycin, polymyxin, streptotrichin, viomycin and chloromycetin to be effective to E.amylovora in laboratory conditions. Penicillin didn’t inhibite growth of E.amylovora in vitro according to Billing et al. (1961). On the other hand, Rudolph (1946) found penicillin to inhibit pathogen in vitro, but its control of Fire blight failed in the field experiments. Martinec and Kocur (1964) found that all 49 tested strains of E.amylovora were sensitive to chloramphenicol, erytromycin, neomycin, streptomycin and teracycline, while 24 were sensitive to chlorotetrcycline. Although many antibiotics inhibit growth of E.amylovora in vitro, only a few have practical value for the field application (mainly streptomycin and sporadicly oxytetracycline and kasugamycin were used).
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Ekološke i epidemiološke osobine
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Dejstvo raznih temperatura i padavi
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Uticaj hranljive podloge i temperat
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Anatomsko-histološke karakteristik
Page 37 and 38: Anatomsko-histološke karakteristik
Page 45 and 46: Odgajivačke odlike izolata Leucost
Page 61 and 62: Uticaj načina zaštite semena ozim
Page 71 and 72: Razvoj mikoparazita Colletrichum gl
Page 83 and 84: Chaenomeles japonica and Cotoneaste
Page 87: Chaenomeles japonica and Cotoneaste
Page 93 and 94: 97 I N M E M O R I A M Dr ANTON ZAB
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