kvalitet

36 Jelica Balaž i sar.
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(Primljeno: 05.02.2007.)
(Prihvaćeno: 08.08.2007.)
INFLUENCE OF NUTRIENT MEDIUM AND TEMPERATURES ON
THE ANAMORPH DEVELOPMENT IN-VITRO OF THE FUNGUS
APIOGNOMONIA VENETA, THE PLANE TREES PATHOGEN
BALAŽ JELICA, ARSENIJEVIĆ MOMČILO, POPOVIĆ TATJANA
Faculty of Agriculture, Department of Plant Protection, 21000 Novi Sad
E-mail: marsa@neobee.net
Summary
The effect of nutrient media and various temperatures on the Apiognomonia
veneta (Sacc. & Speg.) Höhn. development in-vitro has not been investigated in
more details, according to available literature.
Results of our investigation were shown that only anamorph of the fungus
has been developed in-vitro. The best media for colonies growth were malt agar,
potato dextrose agar and sour synthetic medium. The prune agar was unfavorable
for pathogen development.
From the four isolates investigated (Pl-57, Pl-58, Pl-61 and Pl-62), the
colonies of Pl-57 and Pl-58 were the smaller on malt and onion agar than Pl-61
and Pl-62 one (Table 1).
But onion and malt agar stimulated conidiomata production which don't
appeared on sour synthetic and dry plum agar (Table 2).
The conidia production of weak degree were only on onion, PDA and
malt agar by Pl-61 and Pl-62 isolates exept Pl-62 one which conidia formed most
intensively (Table 2).
As it was shown nutrient media more influenced on colony growth than
conidia production (Tables 1 and 2). That can be probably explane by presence of
convenient substances in tissues of plane trees in-vivo than in artificial media for
pathogen development in-vitro.