Laboratory Audit Checklist
Laboratory Audit Checklist
Laboratory Audit Checklist
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** Questions on Protocol<br />
<strong>Laboratory</strong> <strong>Audit</strong> <strong>Checklist</strong><br />
Name of PI:<br />
Room Number:<br />
Name/type of agent<br />
Is the agent replication defective?<br />
Biosafety Level<br />
NIH Guideline Category<br />
Agent received from:<br />
Commercial Source<br />
UK Researcher<br />
Other<br />
Quantity of agent to be produced<br />
Quantity of agent to be stored<br />
Are toxins used in this research?<br />
Copy of approved protocol in lab that describes agent<br />
being studied?<br />
YES NO N/A Comments<br />
Date:<br />
Protocol Specific Information<br />
Name:<br />
Describe:<br />
Facilities and Practices<br />
Does laboratory director establish policy/procedures<br />
and advise personnel of hazards and entry<br />
requirements?<br />
Access to laboratory restricted?<br />
Access restricted to stored viable materials?<br />
The lab is under negative pressure if working with<br />
airborne agents<br />
Fire extinguishers present and inspected?<br />
Chemical spill supplies available?<br />
Are gas cylinders secured and capped?<br />
First aid kit readily available?<br />
Are spills and accidents reported to the lab director?<br />
Separate desk space<br />
Access doors properly labeled<br />
Emergency numbers posted by phone?<br />
Folding step stool available?<br />
Emergency response guide on/near lab door?<br />
Eating and drinking permitted in lab?<br />
Food stored outside work area in designated<br />
refrigerator?<br />
Sink for handwashing?<br />
Liquid hand soap available at sink?<br />
Paper towels available at sink?<br />
Do personnel wash hands after handling viable<br />
materials?<br />
Is emergency shower/eyewash available?<br />
Do lab personnel flush eyewash regularly?<br />
Are eyewash and shower tested by PPD?<br />
Is proper disinfectant available?<br />
List:<br />
Are work surfaces decontaminated once a day?<br />
Are work surfaces decontaminated after spill?<br />
Cultures, stocks, and other wastes decontaminated<br />
before disposal?<br />
Does the lab operate autoclave?<br />
Autoclave location<br />
Is autoclave QC log kept?<br />
Is the autoclave monitored monthly with biological<br />
spore test?<br />
Who is responsible for overseeing the operation of<br />
the autoclave<br />
Are appropriate autoclave bags used?<br />
Plastic substituted for glass when possible?<br />
Broken glass not handled directly, but by mechanical<br />
means?<br />
Proper broken glass disposal?<br />
Describe:<br />
Date last tested:<br />
Date last tested:<br />
Autoclave<br />
Bleach<br />
Other:<br />
Name:<br />
Type: Red<br />
Orange<br />
Clear
Used needles, syringes, and other sharps placed in<br />
appropriate containers for disposal?<br />
Written biohazard disposal procedures in safety<br />
manual?<br />
Biological Safety cabinets used whenever procedures<br />
with potential for creating infectious aerosols or<br />
splashes are conducted?<br />
Are UV lights in the BSC?<br />
Are they used properly?<br />
Is the staff trained for proper use?<br />
Is this training documented?<br />
Are potentially infectious materials centrifuged?<br />
Protective clothing/personal protective equipment<br />
present and used (disposable lab coats)<br />
Disposable gloves are not reused<br />
Are materials placed in appropriate containers for<br />
transport?<br />
Vacuum line protected in place?<br />
Class<br />
Certified by<br />
Date<br />
Type<br />
Safety containment cups<br />
Sealed rotors with O-rings<br />
Other<br />
Latex<br />
Nitrile<br />
Liquid disinfectant trap and HEPA<br />
air filter?<br />
Aware of Export Controls? *<br />
** must be present in lab; may<br />
NIH guidelines in lab<br />
bookmark on computer<br />
*** must be present in lab; may<br />
CDC BMBL in lab?<br />
bookmark on computer<br />
Rev 1/21/05<br />
Commerce Dept. Export Control: http://206.151.87.67/docs/Export%20Controls.pdf<br />
CDC BMBL: http://www.cdc.gov/od/ohs/pdffiles/4th%20BMBL.pdf<br />
NIH Guidelines: http://www4.od.nih.gov/oba/rac/guidelines_02/NIH_Gdlnes_lnk_2002z.pdf
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