The Journal of Research ANGRAU
Contents of 41(1) 2013 - acharya ng ranga agricultural university Contents of 41(1) 2013 - acharya ng ranga agricultural university
EFFECT OF SEED PRIMING ON BIOCHEMICAL CHANGES DURING STORAGE Table 1. Effect of seed priming on á-amylase activity (mmol g -1 fr wt) of maize hybrids during storage S.No. Hybrid ‘0’ Month 2 Months after storage Treat ment Control HYDRO PE G KNO 3 Mean Contr ol HYDRO PEG KNO 3 Mean 1 AH 122 5.07 5.15 5.10 5.13 5.11 4.95 5.03 4.98 5.00 4.99 2 SMH 9 5.23 5.30 5.27 5.31 5.28 5.14 5.24 5.17 5.21 5.19 3 DHM 117 5.30 5.40 5.34 5.37 5.35 5.22 5.31 5.25 5.27 5.26 4 DHM 111 5.13 5.20 5.14 5.18 5.15 5.01 5.10 5.05 5.07 5.06 5 DHM 115 5.17 5.26 5.20 5.23 5.22 5.07 5.17 5.10 5.14 5.12 Mean 13.93 5.17 5.25 5.21 5.22 5.21 5.08 5.17 5.11 5.14 Hybrid (H) Treatment (T) HxT Hybrid (H) Treatment (T) S.Em± 0.004 0.004 0.009 0.002 0.002 0.004 CD at 5% 0.013 0.012 0.027 0.006 0.005 0.012 HxT S.No . Treat ment Hybrid 4 Months after storage 6 Months after storage Control HYDRO PE G KNO 3 Mean Contr ol HYDRO 1 AH 122 3.73 3.82 3.77 3.80 3.78 1.81 1.90 1.84 1.87 1.85 2 SMH 9 3.88 4.12 3.97 4.03 4.01 2.02 2.14 2.05 2.07 2.07 PEG KNO 3 Mean 3 DHM 117 4.01 4.37 4.04 4.07 4.12 2.08 2.17 2.14 2.15 2.14 4 DHM 111 3.81 3.89 3.83 3.85 3.85 1.88 1.97 1.91 1.94 1.92 5 DHM 115 3.86 3.95 3.90 3.93 3.91 1.94 2.11 1.98 2.01 2.03 Mean 11.26 3.87 4.03 3.90 3.93 3.93 1.95 2.10 1.98 2.01 Hybrid (H) Treatment (T) HxT Hybrid H) Treatment (T) S.Em± 0.003 0.0032 0.007 0.006 0.005 0.012 CD at 5% 0.010 0.0091 0.020 0.018 0.016 0.036 HxT Sundareshwaran (2010) who evaluated the influence of seed priming on biochemical parameters of fresh and aged seeds of maize hybrid (COH(M) 5) and its parental lines UMI 285 (female) and UMI 61 (male). Seeds were soaked in water @ 1% KH 2 PO 4 , 3% KNO 3 and 2 % CaCl 2 solution for 6 hrs. The results revealed that all the seed priming treatments affected the biochemical activity of seeds. However, seed priming with 1% KH 2 PO 4 for 6 h significantly increased the protein content, á-amylase and dehydrogenase activities in both fresh and aged seed lots of all the three genotypes. The results are also in conformity with Sathish and Sundareshwaran (2010) and Wattanakulpakin (2012). The peroxidase activity was observed at different months of storage (Table 2). The hybrid DHM 117 showed maximum peroxidase activity with hydropriming indicating the treatment is the most reliable technique for increasing peroxidase activity there by increasing the vigour during storage. The significant interaction between hybrids and treatments was observed at all periods of storage. Priming 72
KUMAR et al Table 2. Effect of seed priming on peroxidase activity (EU/Litre) of maize hybrids during storage S. No. Hy brid Treatm ent 1 AH 2 SMH ‘0’ Month 2 Months after storage Control HYDRO PEG KNO 3 Mean Con trol HYDRO PEG KNO 3 Mean 122 86654.6 87252.0 86854.0 87054.0 86953.6 66346.0 66942.0 66545.0 66746.00 66644.75 9 88376.0 88971.0 88574.0 88771.0 88673.0 67671.0 68272.0 68203.3 68071.00 68054.33 3 DHM 117 88966.0 89566.0 89161.0 89369.0 89265.5 68232.0 68834.0 68435.0 68632.00 68533.25 4 DHM 111 87215.0 87816.0 87417.0 87616.0 87516.0 66545.0 67142.0 66745.3 66943.00 66843.83 5 DHM 115 87983.0 88380.0 87988.0 88183.0 88133.5 67107.0 67706.0 67305.0 67508.00 67406.50 Mean 13.93 87838.8 88397.0 87998.8 88198.6 88108.3 67180.2 67779.2 67446.73 67580.00 Hybrid(H) Treatment(T) HxT Hybrid(H ) Treatment(T) S.Em± 0.77 0.69 1.54 0.52 0.46 1.04 CD (0.05) 2.21 1.98 4.43 1.50 1.34 3.01 HxT S.No. Hybri d Treat ment 1 AH 122 2 SMH 9 3 DHM 117 4 DHM 111 5 DHM 115 4 Months after storage 6 Months after storage Control HYDRO PEG KNO 3 Mean Con trol HYDRO PEG KNO 3 Mean 43105 43702 43306 43508.0 43405.3 17003 17601.3 17202 17407 17303.3 44793 45394 44991 45191.7 45092.3 18694 19293 18894 19097 18994.5 45352 45958 45557 45756.0 45655.8 19256 19857 19456 19652 19555.3 43663 44266 43867 44065.3 43965.3 17564 18165 12433 17961 16530.8 44235 44830 44438 44632.0 44533.8 18128 18729 18328 18524 18427.3 Mean 44229 44830 44431.8 44630.4 Hybrid (H) Treatment (T) HxT 4453 0.5 18129 18729 Hybrid (H) 17262. 6 Treatment (T) 18528.2 S.Em± 0.85 0.76 1.71 0.65 0.58 1.30 CD at 5% 2.45 2.19 4.91 1.86 1.67 3.74 HxT treatments showed superior values in comparision to control. Syed et al., (2010) revealed that the activity of catalase and peroxidase during accelerated ageing and repair priming treatment of maize (Zea mays L.) seeds. Seed priming with KNO 3 was performed at different concentrations of (0.5, 1, 2.5 and 4%) and seeds were soaked for 8, 12 and 24 h in each individual concentration and the results showed that there was significant difference for duration of ageing treatment on germination characteristics of maize seeds. Increasing ageing duration resulted higher reduction in germination characteristics. KNO 3 had positive effects on seed germination of aged seed. This was higher in application of 0.5% KNO 3 73
- Page 21 and 22: LAKSHMI et al Changes in C/N ratio
- Page 23 and 24: LAKSHMI et al Table 3. Changes in h
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- Page 39 and 40: VEMANNA et al grain yield per plant
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- Page 45 and 46: RAMANA et al RESULTS AND DISCUSSION
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- Page 51 and 52: RAJANNA et al the present findings
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- Page 55 and 56: NARASIMHA et al Maynard, L., Lossli
- Page 57 and 58: RAMANA et al with small follicles m
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- Page 63 and 64: KIRTHY et al Akhtar et al., 2008; S
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- Page 67 and 68: SANDYARANI et al Weed parameters li
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- Page 95 and 96: VEMANNA et al Table 1. Discriminant
- Page 97 and 98: VEMANNA et al total biomass, fresh
- Page 99 and 100: RAO et al Table 1. Plant height, nu
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- Page 103 and 104: DEVI et al S.No Category Frequency
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- Page 107 and 108: NIRMALA and VASANTHA earliness in a
- Page 109 and 110: NIRMALA and VASANTHA adopted this t
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- Page 117 and 118: NIRMALA et al Table 3. Cluster mean
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KUMAR et al<br />
Table 2. Effect <strong>of</strong> seed priming on peroxidase activity (EU/Litre) <strong>of</strong> maize hybrids during storage<br />
S.<br />
No.<br />
Hy<br />
brid<br />
Treatm<br />
ent<br />
1 AH<br />
2 SMH<br />
‘0’ Month 2 Months after storage<br />
Control HYDRO PEG KNO 3 Mean<br />
Con<br />
trol<br />
HYDRO PEG KNO 3 Mean<br />
122 86654.6 87252.0 86854.0 87054.0 86953.6 66346.0 66942.0 66545.0 66746.00 66644.75<br />
9 88376.0 88971.0 88574.0 88771.0 88673.0 67671.0 68272.0 68203.3 68071.00 68054.33<br />
3 DHM<br />
117 88966.0 89566.0 89161.0 89369.0 89265.5 68232.0 68834.0 68435.0 68632.00 68533.25<br />
4 DHM<br />
111 87215.0 87816.0 87417.0 87616.0 87516.0 66545.0 67142.0 66745.3 66943.00 66843.83<br />
5 DHM<br />
115 87983.0 88380.0 87988.0 88183.0 88133.5 67107.0 67706.0 67305.0 67508.00 67406.50<br />
Mean 13.93 87838.8 88397.0 87998.8 88198.6 88108.3 67180.2 67779.2 67446.73 67580.00<br />
Hybrid(H) Treatment(T) HxT<br />
Hybrid(H<br />
)<br />
Treatment(T)<br />
S.Em± 0.77 0.69 1.54 0.52 0.46 1.04<br />
CD (0.05) 2.21 1.98 4.43 1.50 1.34 3.01<br />
HxT<br />
S.No.<br />
Hybri<br />
d<br />
Treat<br />
ment<br />
1 AH<br />
122<br />
2 SMH<br />
9<br />
3 DHM<br />
117<br />
4 DHM<br />
111<br />
5 DHM<br />
115<br />
4 Months after storage 6 Months after storage<br />
Control HYDRO PEG KNO 3 Mean<br />
Con<br />
trol<br />
HYDRO PEG KNO 3 Mean<br />
43105 43702 43306 43508.0 43405.3 17003 17601.3 17202 17407 17303.3<br />
44793 45394 44991 45191.7 45092.3 18694 19293 18894 19097 18994.5<br />
45352 45958 45557 45756.0 45655.8 19256 19857 19456 19652 19555.3<br />
43663 44266 43867 44065.3 43965.3 17564 18165 12433 17961 16530.8<br />
44235 44830 44438 44632.0 44533.8 18128 18729 18328 18524 18427.3<br />
Mean 44229 44830 44431.8 44630.4<br />
Hybrid<br />
(H)<br />
Treatment (T)<br />
HxT<br />
4453<br />
0.5<br />
18129 18729<br />
Hybrid<br />
(H)<br />
17262.<br />
6<br />
Treatment (T)<br />
18528.2<br />
S.Em± 0.85 0.76 1.71 0.65 0.58 1.30<br />
CD at 5% 2.45 2.19 4.91 1.86 1.67 3.74<br />
HxT<br />
treatments showed superior values in comparision<br />
to control. Syed et al., (2010) revealed that the<br />
activity <strong>of</strong> catalase and peroxidase during accelerated<br />
ageing and repair priming treatment <strong>of</strong> maize (Zea<br />
mays L.) seeds. Seed priming with KNO 3<br />
was<br />
performed at different concentrations <strong>of</strong> (0.5, 1, 2.5<br />
and 4%) and seeds were soaked for 8, 12 and 24 h in<br />
each individual concentration and the results showed<br />
that there was significant difference for duration <strong>of</strong><br />
ageing treatment on germination characteristics <strong>of</strong><br />
maize seeds. Increasing ageing duration resulted<br />
higher reduction in germination characteristics. KNO 3<br />
had positive effects on seed germination <strong>of</strong> aged<br />
seed. This was higher in application <strong>of</strong> 0.5% KNO 3<br />
73
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