The Journal of Research ANGRAU

EFFECT OF SEED PRIMING ON BIOCHEMICAL CHANGES DURING STORAGE
Table 1. Effect of seed priming on á-amylase activity (mmol g -1 fr wt) of maize hybrids during storage
S.No.
Hybrid ‘0’ Month 2 Months after storage
Treat
ment
Control
HYDRO
PE
G
KNO 3 Mean
Contr
ol
HYDRO PEG KNO 3 Mean
1 AH 122 5.07 5.15 5.10 5.13 5.11 4.95 5.03 4.98 5.00 4.99
2 SMH 9 5.23 5.30 5.27 5.31 5.28 5.14 5.24 5.17 5.21 5.19
3 DHM
117 5.30 5.40 5.34 5.37 5.35 5.22 5.31 5.25 5.27 5.26
4 DHM
111 5.13 5.20 5.14 5.18 5.15 5.01 5.10 5.05 5.07 5.06
5 DHM
115 5.17 5.26 5.20 5.23 5.22 5.07 5.17 5.10 5.14 5.12
Mean 13.93 5.17 5.25 5.21 5.22 5.21 5.08 5.17 5.11 5.14
Hybrid (H)
Treatment
(T)
HxT
Hybrid
(H)
Treatment (T)
S.Em± 0.004 0.004 0.009 0.002 0.002 0.004
CD at 5% 0.013 0.012 0.027 0.006 0.005 0.012
HxT
S.No
. Treat
ment
Hybrid 4 Months after storage 6 Months after storage
Control
HYDRO
PE
G
KNO 3
Mean
Contr
ol
HYDRO
1 AH 122 3.73 3.82 3.77 3.80 3.78 1.81 1.90 1.84 1.87 1.85
2 SMH 9 3.88 4.12 3.97 4.03 4.01 2.02 2.14 2.05 2.07 2.07
PEG
KNO
3
Mean
3 DHM
117 4.01 4.37 4.04 4.07 4.12 2.08 2.17 2.14 2.15 2.14
4 DHM
111 3.81 3.89 3.83 3.85 3.85 1.88 1.97 1.91 1.94 1.92
5 DHM
115 3.86 3.95 3.90 3.93 3.91 1.94 2.11 1.98 2.01 2.03
Mean 11.26 3.87 4.03 3.90 3.93 3.93 1.95 2.10 1.98 2.01
Hybrid
(H)
Treatment
(T)
HxT
Hybrid
H)
Treatment (T)
S.Em± 0.003 0.0032 0.007 0.006 0.005 0.012
CD at 5% 0.010 0.0091 0.020 0.018 0.016 0.036
HxT
Sundareshwaran (2010) who evaluated the influence
of seed priming on biochemical parameters of fresh
and aged seeds of maize hybrid (COH(M) 5) and its
parental lines UMI 285 (female) and UMI 61 (male).
Seeds were soaked in water @ 1% KH 2
PO 4
, 3% KNO 3
and 2 % CaCl 2
solution for 6 hrs. The results revealed
that all the seed priming treatments affected the
biochemical activity of seeds. However, seed priming
with 1% KH 2
PO 4
for 6 h significantly increased the
protein content, á-amylase and dehydrogenase
activities in both fresh and aged seed lots of all the
three genotypes. The results are also in conformity
with Sathish and Sundareshwaran (2010) and
Wattanakulpakin (2012).
The peroxidase activity was observed at
different months of storage (Table 2). The hybrid DHM
117 showed maximum peroxidase activity with
hydropriming indicating the treatment is the most
reliable technique for increasing peroxidase activity
there by increasing the vigour during storage. The
significant interaction between hybrids and treatments
was observed at all periods of storage. Priming
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