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The Journal of Research ANGRAU

Contents of 41(1) 2013 - acharya ng ranga agricultural university

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<strong>Research</strong> Notes<br />

J.Res. <strong>ANGRAU</strong> 41(1) 66-69, 2013<br />

EFFECT OF SEED PRIMING ON BIOCHEMICAL CHANGES DURING SEED<br />

STORAGE OF MAIZE (ZEA MAYS L.) HYBRIDS<br />

M. RAM KUMAR, P. S. RAO, V. PADMA and K. V. RADHA KRISHNA<br />

Department <strong>of</strong> Crop Physiology, College <strong>of</strong> Agriculture<br />

Acharya NG Ranga Agricultural University, Rajendranagar, Hyderabad-500 030<br />

Date <strong>of</strong> Receipt : 04.06.2012 Date <strong>of</strong> Acceptance : 08.02.2013<br />

Maize is one <strong>of</strong> the most important cereal<br />

crops in the world. It is widely grown twice a year in<br />

certain agro climatic zones <strong>of</strong> India and the world as<br />

a staple food. Only 20-25 percent quantity <strong>of</strong> the seed<br />

is invariably stored and used by farmers in<br />

subsequent seasons. <strong>The</strong> remaining 75-80 percent<br />

<strong>of</strong> the seed quantity is transported to market during<br />

the first season itself to meet the demand <strong>of</strong> the<br />

farmers. <strong>The</strong> challenging task to overcome problems<br />

in process <strong>of</strong> the seed storage is the seed<br />

deterioration (viz., physiological or biochemical<br />

changes) which ultimately leads to loss <strong>of</strong> its vigour<br />

and viability. Priming is also thought to increase<br />

enzyme activity and counteract the effects <strong>of</strong> lipid<br />

peroxidation (Saha et al., 1990). During priming de<br />

novo synthesis <strong>of</strong> á-amylase is also documented<br />

(Lee and Kim, 2000). Metabolic activities in seeds<br />

increase with á-amylase activity, indicating higher<br />

seed vigour. Rapid and uniform field emergence, are<br />

two essential prerequisites to increase yield, quality<br />

and ultimately pr<strong>of</strong>its in crop production. Uniformity<br />

and percentage <strong>of</strong> seedling emergence <strong>of</strong> directseeded<br />

crops have a major impact on final yield and<br />

quality.<br />

<strong>The</strong> medium vigour (Six month old) seed lots<br />

<strong>of</strong> five maize hybrids viz., AH 122, SMH-9, DHM<br />

111, DHM 115 and DHM 117 having germination up<br />

to minimum seed certification standard (MSCS) were<br />

dried to 9% moisture are used for the study during<br />

2010-11. <strong>The</strong> seeds were subjected to priming at 25 o C<br />

with distilled water (T 1<br />

), PEG - 1.2 MPa (T 2<br />

), KNO 3<br />

@ 0.5% (T 3<br />

) along with untreated control (To). <strong>The</strong><br />

invigoration treatments were given to the seeds<br />

by soaking in same quantity <strong>of</strong> water/aqueous<br />

solution <strong>of</strong> chemical (1: 1 seed and water/solution)<br />

for 18 hours at ambient temperature followed by drying<br />

back to original moisture content under shaded<br />

condition. Immediately after drying the treated seeds<br />

were stored in moisture impervious containers at<br />

ambient condition. <strong>The</strong> seed samples (primed and<br />

unprimed) were drawn at 0, 2, 4 and 6 months after<br />

treatment for studying the enzymatic changes during<br />

storage. <strong>The</strong> fresh seed sample <strong>of</strong> 0.5 g was<br />

homogenized with 5ml <strong>of</strong> chilled citrate buffer (pH<br />

5.0) and centrifuged at 10,000 rpm for 15 minutes.<br />

<strong>The</strong> supernatant was used for assessing the<br />

activity <strong>of</strong> á-amylase as per the procedure described<br />

by Choi et al. (1996). Enzyme extract for peroxidase<br />

was prepared by first freezing the weighed amount<br />

<strong>of</strong> seed sample (1g) in liquid nitrogen to prevent<br />

proteolytic activity followed by grinding with 10 ml<br />

extraction buffer (0.1M phosphate buffer, pH<br />

7.5,containing 0.5 mM EDTA). Brie was passed<br />

through four layers <strong>of</strong> cheese cloth and filtrate was<br />

centrifuged for 20 min. at 15000 G and the supernatant<br />

was used as enzyme extract. <strong>The</strong> enzyme extract<br />

was used for assesing the acivity <strong>of</strong> peroxidase<br />

described by Castillo et al. (1984).<br />

<strong>The</strong> α - amylase activity (mmol g -1 fr wt)<br />

was reported to be significantly different among<br />

treatments and hybrids. <strong>The</strong>re was a significant<br />

interaction between hybrids and treatments at all<br />

duration <strong>of</strong> time after storage (Table 1). Among the<br />

five maize hybrids DHM 117 primed with water<br />

(Hydropriming) recorded maximum value (2.17 mmol<br />

g -1 ) <strong>of</strong> á- amylase activity while unprimed seeds <strong>of</strong><br />

AH 122 recorded least value (1.81 mmol g -1 ).<br />

Significant interaction between hybrids and treatments<br />

at all time intervals <strong>of</strong> germination at all the months<br />

<strong>of</strong> storage was noticed. <strong>The</strong> findings are in conformity<br />

with the studies <strong>of</strong> Sung and Chang (1993); where in<br />

the hydropriming was superior to PEG 6000 in sh-2<br />

corn hybrid and was more effective in improving the<br />

emergence which resulted due to higher á- amylase<br />

and â-amylase activities. Similarly a laboratory<br />

study was conducted by Sathish and<br />

email: sampalrao@gmail.com<br />

71

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