The Journal of Research ANGRAU

More documents

Recommendations

Info

J.Res. ANGRAU 41(1) 39-41, 2013 ESTRUS SYNCHRONIZATION RESPONSE AND FERTILITY RATE FOLLOWING TREATMENT WITH PGF 2 α AND GnRH IN ACYCLIC LACTATING ONGOLE COWS K.VENKATA RAMANA, K.SADASIVA RAO, K.SUPRIYA and N.RAJANNA Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science, Sri Venkateswara Veterinary University, Rajendranagar – 500 030 Date of Receipt : 26.12.2012 Date of Acceptance : 04.02.2013 ABSTRACT Estrus synchronization response and fertility following Ovsynch and double prostaglandin injection protocols in post partum Ongole cows was studied. A total of 50 Ongole parous cows above 60 days postpartum were divided in to two treatment groups consisting of 22 cows in each and 6 cows in control group. The follicular dynamics were monitored every day by ultrasonography till the ovulation. The emergence of follicular wave was observed on 1.33, 3-4 days in Ovsynch and double PG groups, respectively. The size of dominant follicle found to be 12.48 ± 0.57 mm in Ovsynch and control group whereas 10.00 ± 0.78 mm in double PG group. In Ovsynch group estrus response was noticed in 100 per cent cows and estrus was recorded after 50 to 80 hours of PGF 2 á injection with the duration of 16.28 ± 2.36 hrs While in double PG the duration of estrus and mean estrous cycle length recorded as 14.20 ± 2.56 hrs and 21.50 ± 0.21 days, respectively. The ovulations were noticed after 1.50 ± 0.22 days of 2 nd GnRH injection with conception rate of 54.54 per cent in Ovsynch. The mean service period found to be 81.18 ± 1.62 days in lactating multiparous Ongole cows. It may be concluded that double injection of prostaglandin has better conception rate than Ovsynch protocol and both the treatments reduced the service period around 80 days when compare with control group, there by reduced the calving interval in lactating Ongole cows. Ongole breed of cattle is dual purpose (milk and draught) cattle, due to their adaptability traits, superior production capacity and high disease resistance under harsh tropical conditions. However, certain reproductive impediments like long service period which results in to long calving intervals, nocturnal incidence of estrus with shorter duration are limiting the economic use of this cattle breed. The postpartum ovarian inactivity could be due to suckling induced inhibition of the LH surge (Gumen et al, 2003 and Naidu et al, 2007).Hence, an attempt was made to study the follicular dynamics during the postpartum and to enhance the fertility with PGF 2 α and GnRH hormonal protocols in lactating Ongole cows. MATERIALS AND METHODS A total of fifty postpartum lactating Ongole cows in their 2 nd to 5 th lactation maintained under standard feeding and management stationed at Cattle Project, Live Stock Research Station, Lam Farm, Guntur were included in this study. The cows were randomly divided in to two groups consisting of 22 cows in each treatment group and six cows in control group. Estrus synchronization was performed by Ovsynch protocol (Treatment-I) i.e., day 0 Receptal 10 g i.m, day 7 Lutalyse 25 mg i.m and again on day 9 Receptal 10 g i.m. Where as in double injection of prostaglandin group (Treatment-II) the first injection (Lutalyse 25 mg i.m) was given on day ‘0’ and 2 nd injection was given on day 12 th . The inseminations were carried out on observed estrus in both the groups. The non returned cows were examined for pregnancy per rectally between day 60 to 70 post insemination. Ovarian structures in six cows in each treatment groups and control group were monitored daily by using a real time B-mode ultrasound scanner with a trans rectal linear-array transducer from day ‘0’ of GnRH injection to till the end of induced estrus with ovulation to assess the fate of first wave dominant follicle and the emergence of subsequent wave. The scanning was also done in control group and ovarian changes and estrus response was recorded and compared with treatment groups during the treatment period. The data was analyzed by Minitab ®(16) (2012) software. email: kvr_vetgyn@yahoo.in 44
RAMANA et al RESULTS AND DISCUSSION In Ovsynch protocol the first injection of GnRH is designed to induce ovulation and formation of a new or accessory corpus luteum and a new follicular wave. In the present investigation ultrasound scanning of ovaries was done every day after injection of GnRH induced ovulation and till the formation of corpus luteum. However, follicular wave emerge was noticed in all the six cows on day 1.33+0.21. The dominant follicle resulted from this wave emergence grew up to 12.48+0.57 mm by day 7.6 with a growth rate of 1.66+0.26 mm per day. This is in agreement with the reports of Mishra et al.(2002) and Souza et al. (2006) in Sahiwal cows with GnRH. However, Krishna Mohan et al. (2010) reported much lesser size (9.17 + 0.27 mm) dominant follicle in Sahiwal cows. All the cows with a corpus luteum which were administered with luteolytic dose (25 mg of Dinoprost Tromethamine) on day 7 were in estrus within 2.33+0.21 days. The dominant follicle of all the six cows was ovulated with the second dose of GnRH on day 9 of the luteal phase. The ovulation recorded was 100 per cent with GnRH treatment which is in agreement with Gumen et al. (2003). This could be due to larger size of the dominant follicle measuring more than 9 mm will potentially ovulate in the GnRH treatment animals (Adams 1992). Ultrasound monitoring of the ovulated cows had shown a corpus luteum of 16.50+0.30 mm on day 10 following the induced estrus. Out of 22 postpartum lactating cows, 8 cows showed estrus after 1 st GnRH injection and the cows exhibited normal estrus except two which had weak estrus following PGF 2 α administration on day 7 of the treatment. Second dose of GnRH was administered on day 9 and all the cows inseminated at the observed estrus. The estrus response recorded in this study was in agreement with Mialot et al. (1998) and Vasconcelos et al. (1999). In the present study the conception rate found to be 54.54+0.36 per cent. The ultrasound scanning of PGF 2 α treated cows revealed a dominant follicle of 10.00 + 0.78 mm within 3-4 days after the PGF 2 α administration, which is in agreement with Alan et al. (2003) and Naidu et al. (2010) in Nelore cows. Out of 22 cows treated with double injections of prostaglandins 18 exhibited estrus within 3.66+0.21 days. The time of onset of estrus following PGF 2 α injection recorded in this study is corroborating to the findings of Alves et al. (2002) in Gir cows. In the present study the conception rate found to be 67.00 +0.26 per cent. Naidu et al. (2006) has reported higher conception rate (90.0%) than the present findings in Ongole cows, which might be due to better synchrony of ovulation and fertilization as the existing follicles were influenced the next wave of follicles during induction. All the treated postpartum lactating ongole cows in Ovsynch and double PG groups exhibited 100 and 82.5 per cent of estrus, respectively. The reason for varied estrus response rate between different treatment groups might be due to the difference in the treatment protocols and due to, presence of too small dominant follicles (< 9 mm) at the time of second PGF 2 injection (Rivera et al. 1998). In the control group the mean time required for conception after calving was recorded as 163.60+10.72 (95-397) days, which is less than the previous studies (Venkateswarulu, 1971; Rao et al, 1985; Acharya & Bhat 1990; Ravi Kiran et al. 1995; Rao et al. 2001and Naidu et.al,.,.2010). The service period of cows in the treatment groups resulted a reduction of approximately 85 days compared to control group cows. Hence, it is concluded that the estrus synchronization with GnRH and PGF2 gives better results and would reduce the calving to service period drastically and beneficial to the farmers in dairy industry. ACKNOWLEDGEMENT The authors express their sincere gratitude to the SVVU, Tirupati for providing facilities to carry out the research work at Cattle Project, Live Stock Research Station, Lam Farm, Guntur.A.P. 45
Page 1 and 2: 1
Page 3 and 4: CONTENTS PART I : PLANT SCIENCE Eff
Page 5 and 6: J.Res. ANGRAU 41(1) 1-4, 2013 EFFEC
Page 7 and 8: EFFECT OF FOLIAR APPLICATION OF NPK
Page 9 and 10: J.Res. ANGRAU 41(1) 5-13, 2013 NUTR
Page 11 and 12: NUTRIENT UPTAKE BY RICE CROP UNDER
Page 19 and 20: LAKSHMI et al Fig 1. Changes in C/N
Page 21 and 22: LAKSHMI et al Changes in C/N ratio
Page 23 and 24: LAKSHMI et al Table 3. Changes in h
Page 25 and 26: J.Res. ANGRAU 41(1) 20-29, 2013 INF
Page 27 and 28: PRASAD and PRASADINI of bulk densit
Page 29 and 30: PRASAD and PRASADINI to as high as
Page 31 and 32: PRASAD and PRASADINI Table 4 . Infl
Page 33 and 34: PRASAD and PRASADINI Table 8. Influ
Page 35 and 36: J.Res. ANGRAU 41(1) 30-38, 2013 GEN
Page 37 and 38: VEMANNA et al The range in mean val
Page 39 and 40: VEMANNA et al grain yield per plant
Page 41 and 42: VEMANNA et al 41
Page 43: VEMANNA et al Singh, S. P and Khan,
Page 47 and 48: J.Res. ANGRAU 41(1) 42-46, 2013 A S
Page 49 and 50: RAJANNA et. al. Table 2. Reasons fo
Page 51 and 52: RAJANNA et al the present findings
Page 53 and 54: NARASIMHA et al Table 1. Proximate
Page 55 and 56: NARASIMHA et al Maynard, L., Lossli
Page 57 and 58: RAMANA et al with small follicles m
Page 59 and 60: RAMANA et al Characteristics of fol
Page 61 and 62: Research Notes J.Res. ANGRAU 41(1)
Page 63 and 64: KIRTHY et al Akhtar et al., 2008; S
Page 65 and 66: KIRTHY et al El Gharras H. 2009. Po
Page 67 and 68: SANDYARANI et al Weed parameters li
Page 69 and 70: SANDYARANI et al Table 2. Influence
Page 73 and 74: KUMAR et al Table 2. Effect of seed
Page 77 and 78: DEEPAK et al Table 2 Price spread a
Page 81 and 82: YAMINI et al Table 3. Estimates of
Page 83 and 84: YAMINI et al coupled with high per
Page 85 and 86: LOKESH et al Table 1. Clustering pa
Page 87 and 88: LOKESH et al Table 4. Mean values o
Page 89 and 90: ABIRAMI et al Socio-economic Impact
Page 91 and 92: ABIRAMI et al socio-economic impact
Page 95 and 96:
VEMANNA et al Table 1. Discriminant
Page 97 and 98:
VEMANNA et al total biomass, fresh
Page 99 and 100:
RAO et al Table 1. Plant height, nu
Page 101 and 102:
Research Notes J.Res. ANGRAU 41(1)
Page 103 and 104:
DEVI et al S.No Category Frequency
Page 105 and 106:
DEVI et al extension contact and ma
Page 107 and 108:
NIRMALA and VASANTHA earliness in a
Page 109 and 110:
NIRMALA and VASANTHA adopted this t
Page 111 and 112:
SUDHARANI et al Table 1. Genotypic
Page 113 and 114:
SUDHARANI et al At genotypic level,
Page 115 and 116:
NIRMALA et al weight of pods per pl
Page 117 and 118:
NIRMALA et al Table 3. Cluster mean
Page 119 and 120:
Page 121 and 122:
Page 123 and 124:
PUNYAVATHI and VIJAYALAKSHMI 6.5 6.
Page 125 and 126:
PUNYAVATHI and VIJAYALAKSHMI REFERE
Page 127 and 128:
KATTEL et al Table 1. Item Analysis
Page 129 and 130:
Page 131 and 132:
RADHIKA et al Thus, the results of
Page 133 and 134:
RAJU et al estimated daily dietary
Page 135 and 136:
RAJU et al Table 3. Physico-chemica
Page 137 and 138:
Page 139 and 140:
MINNIE et al Table 2. Estimates of
Page 141 and 142:
Statement about ownership and other
Page 143 and 144:
GUIDELINES FOR THE PREPARATION OF M
Page 145:
ESSENTIAL REQUIREMENTS FOR CONSIDER
show all

The Journal of Research ANGRAU

Create successful ePaper yourself

Delete template?

Save as template?