TABLE CONTENTS

Identification of Multiple Resistance against Cereal Cyst (Heterodera
filipjevi) and Root Lesion (Pratylenchus thornei and P. neglectus)
Nematodes for International Bread Wheat Improvement
Yorgancilar, A. (1), E. Sahin (2,3), A.T. Kılınç (1), J.M. Nicol (2), G. Erginbas (2), N. Bolat
(1), H. Elekçioğlu (3), A. F. Yıldırım (4), O.Yorgancılar (1) & O.Bilir (1)
(1) Anatolian Agricultural Research Institute P.O. Box.17 26002 Eskişehir, Turkey; (2) CIMMYT (International
Maize and Wheat Improvement Centre), ICARDA CIMMYT Wheat Improvment Program, Ankara, Turkey; (3)
Cukurova University Agricultural Faculty, Plant Protection Department, Adana, Türkiye; (4) Plant Protection
Institute, Ankara
Globally, Cereal Cyst (CCNs) and Root Lesion (RLNs) nematodes are economically
important in wheat production systems. In Turkey, recent surveys have revealed widespread
distribution of both nematodes which commonly occur together. Hence a clear strategy to
identify resistance to both groups has been initiated under a joint Turkish/CIMMYT
International wheat improvement program. More than 500 selected bread wheats from
Turkish National and International (CIMMYT, CIMMYT/ICARDA/Turkey, other foreign
sources) germplasm were screened in order to determine their resistance against CCNs
(Heterodera filipjevi) and RLNs (Pratylenchus thornei & P. neglectus) using local Turkish
isolates of the nematodes. Many of the lines screened were crosses made by breeders to
pyramid sources of resistance from the global literature. Under greenhouse conditions with 7
replicates per line arranged as a Randomised Complete Block Design, an optimized screening
methodology was used for both RLN and CCN. Results were evaluated 9 weeks later after
nematode inoculation. Two partial resistant and susceptible lines were used for each
nematode species as check lines. Plants were grown in the greenhouse at 26 o C with 16 hours
light per day. Data were analyzed with ANOVA using quantitative numbers for each
nematode species. Lines were then ranked according to the checks for partial resistance and
susceptibility. In total 44 sources of resistance were identified – 22 spring and 20 winter.
There were 4 sources of combined nematode resistance in spring wheats, 2 against CCN and
P. thornei, and 2 against P. thornei and P. neglectus. Sources of resistance against individual
nematode species have also been found including 15 sources for CCN (5 spring, 10 winter),
16 for P. thornei (9 spring, 7 winter) and 7 against P. neglectus (4 spring, 3 winter). Eleven
of these lines are sisters. These results indicate the potential to identify nematode resistant
germplasm for use in both International and National breeding programs.
5 th International Congress of Nematology, 2008 31