TABLE CONTENTS

Plant Parasitic Nematodes in Australian Turf
Nambiar, L. & M. Quader
Nematology, Bio-Protection, Department of Primary Industries, Knoxfield Centre, 621 Burwood HWY,
Knoxfield 3180, Victoria, Australia,
A total of 1106 soil samples from turfs (mainly golf and bowling courses) of Victoria (VIC),
New South Wales (NSW), South Australia (SA), Queensland (QLD), Western Australia
(WA) and Tasmania (TAS) were tested for plant parasitic nematodes over the last 10 years
(1997-2007). The data were recorded in diagnostic database of crop health services of DPI,
Victoria. Thirteen types of plant parasitic nematodes were detected from NSW [e.g. spiral
(Helicotylenchus spp., Rotylenchus spp., Scutellonema spp.), stubby-root (Paratrichodorus
spp.), Sheath (Hemicycliophora spp.), sting (Morulaimus sp.), root lesion (Pratylenchus
spp.), root knot Meloidogyne spp.), cyst (Heterodora sp.), ring (Macroposthonia spp.,
Hemicriconemoides spp. Ogma spp.), lance (Hopolaimus spp.), dagger (Xiphinema spp.),
needle (Longidorus spp.), pin (Paratylenchus spp.) and stunt (Tylenchorhynchus spp and
Merlinius spp.)] followed by 12 types from VIC (same as NSW except lance), 12 types form
SA (same as Victoria), 12 types from QLD (same as NSW except pin), 11 types from WA
(same as NSW except lance and needle) and 10 types from TAS (same as NSW except sting,
lance and needle). The severities of infestations were highest in turf courses of NSW
followed by VIC, SA, QLD, WA and TAS. The information is useful to study the pattern and
types of incidence of plant parasitic nematodes in turfs of Australia.
Comparison of the Morphology, Host Range and DNA Sequences of Single
Female Isolates of Pratylenchus cf neglectus.
Nobbs, J.M. (1), V.A. Vanstone (2) & D. Hartley (3)
(1) Plant and Soil Health, South Australian Research and Development Institute, South Australia 5001; (2)
Department Agriculture and Food, Western Australia 6151; 3) CSIRO Plant Industries ACT
Over 500 single female isolates of Pratylenchus cf neglectus were grown in carrot culture
from specimens obtained from different geographic regions of the Western Australian cereal
cropping zone. These isolates were similar to the described species P. neglectus, although
there were differences in morphological and morphometric characters. Since isolates were
reared on carrot disk under controlled conditions, the influence of host and environment was
minimised. The isolates were compared by measurement of 17 morphological characters,
host range tests and DNA sequencing. Significant morphometric differences were found
between the Western Australian isolates and also when compared with a known standard
population of P. neglectus from South Australia. There were also differences in DNA profiles
between selected isolates, but these were not considered significant at the species level.
While there were host differences between isolates, these were only significant for 2 of the 8
hosts tested. These results indicate that while morphological variation between populations of
P. neglectus can be identified, this does not necessarily equate with differences in host range.
The differences in DNA profiles between populations of P. neglectus may reflect the start of
speciation due to selection pressures imposed by hosts and environment.
5 th International Congress of Nematology, 2008 331