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and eggs increased and root weight decreased, due to phytotoxicity. However, at 10 4 ppm, the<br />

total number of eggs per plant was 35.7% lower than control. Caffeine was known for its<br />

activity against snails, but not for phytoparasitic nematodes.<br />

Resistance of Pasture Grasses and Legumes to Radopholus similis<br />

Cobon, J. (1) & A. Pattison (2)<br />

Queensland Department of Primary Industries and Fisheries<br />

(1) 80 Meiers Rd, Indooroopilly, Qld 4068; (2) Centre for Wet Tropics, South Johnstone, Qld 4859.<br />

There is increasing pressure on banana farmers to maintain ground cover around plants to<br />

prevent the movement of soil, nutrients and pesticides from the farm. However, in banana<br />

plantations there is potential for ground cover plants to host Radopholus similis. Therefore,<br />

plants chosen as ground covers need to be agronomically suitable, resistant to R. similis and<br />

hinder the migration of R. similis to the roots of bananas. Screening of potential shade<br />

tolerant grasses and legumes for resistance to R. similis and companion planting with bananas<br />

was conducted in glasshouse experiments.<br />

Selected pasture species were established in pots and inoculated with 500 motile R. similis.<br />

Ten weeks later, nematodes were extracted from the roots. Pasture species, identified as<br />

resistant, were selected for an assay to determine their suitability to prevent R. similis<br />

migrating to the roots of bananas. Mung beans (Vigna radiata) infected with R. similis were<br />

grown in a pot on the outside of the selected pasture species and an in vitro banana plant<br />

grown in the centre of the pot.<br />

All grass species tested were found to be more resistant to R. similis than bananas with<br />

Argentine Bahia (Paspalum notatum) more resistant than signal grass (Brachiaria<br />

humidicola), Rhodes grass (Chloris gayana cv. Katambora) and Sabi grass (Urochloa<br />

mosambicensis). Of the pasture legumes, Butterfly pea (Clitoria ternatea) and Pinto peanut<br />

(Arachis pintoi) were more resistant to R. similis than banana.<br />

Butterfly pea, grown alone or in combination with carpet grass (Axonopus affinis) or Bahia<br />

grass, resulted in lower nematode populations in the roots of bananas. However, the banana<br />

root weight was significantly reduced. Pinto peanuts and Bahia grass, grown separately,<br />

reduced the number of nematodes recovered from the roots but did not reduce the weight of<br />

the banana roots making them better companion plants as ground covers in banana<br />

plantations.<br />

Acknowledgements: Horticulture Australia Limited and Growcom provided funding for this<br />

work under project FR02025.<br />

5 th International Congress of Nematology, 2008 308

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