TABLE CONTENTS

identified TDFs sequences showed identities at amino acid level to known genes and
hypothetical proteins ranging from 62-87%. These included putative transcription regulators,
ubiquitin modifiers, glycosyl transferases and chaperonins.
Combined Use of Three Different Microbial Agents for Management of
Meloidogyne javanica in Tomato
Flor, E. (1), T. Salmerón (1), R. Azcón (2) & M. Talavera (1)
(1) IFAPA Centro Camino de Purchil. Apdo. 2027. 18080 Granada Spain; (2) Estación Experimental Zaidín,
CSIC. Profesor Albareda 1, 18008 Granada, Spain
Root-knot nematodes (Meloidogyne spp.) are the main nematological problem for
horticultural crops in Spain. A great number of microbial antagonists of nematodes have been
investigated for their biological control potential but no one of then has shown enough
efficiency by itself to be considered as an alternative to nematicides in intensive crops. With
the aim of finding a feasible biological control tactic for root-knot nematodes, we have tried
to combine the use of several nematode biocontrol agents, using different biological control
strategies and thus, susceptible of working together. The bacterial hyperparasite Pasteuria
penetrans, which can reduce the infective inoculum of nematodes in soil and its reproduction
potential and a mycorrhizal fungus Glomus mosseae or a PGPR Pseudomonas spp., which
can compensate partially for the damage caused by nematodes and also can induce a systemic
resistance response in the host plant. Pot experiments were carried out in a protected
glasshouse under controlled conditions. In all the experiments, the use of a biocontrol agent
together with an additional bioprotection in the host plant increased plant growth parameters
and reduced the reproduction index in nematodes after 8-10 weeks of growth. Final nematode
densities were only reduced in treatments with P. penetrans. Treatments with G. mosseae
increased plant growth parameters (plant height and weight) by 18-37% and reduced gall
index caused by nematodes (23-45%). Treatments with Pseudomonas increased slightly plant
growth (14-21%) but did not reduce gall index. No interactions were observed among
treatments but protection conferred by the mychorrizal fungus and bacterial hyperparasite
applied together was greater than that conferred by these treatments applied individually.
Pochonia chlamydosporia Reduces the Multiplication Rate of Potato Cyst
Nematodes (Globodera pallida and G. rostochiensis) in Potato Crops in the
UK
Tobin, J.D. (1), P.P.J. Haydock (1), M.C. Hare (1), S.R. Woods (1) & D.H. Crump (2)
(1) Nematology and Entomology Group, Crop and Environment Research Centre, Harper Adams University
College, Newport, Shropshire, TF10 8NB, UK; (2) BioNem Ltd., Caddington, Bedfordshire, LU1 4AS, UK
Two field experiments were undertaken in 2006 and 2007 at different field sites in Shropshire
to assess the effects of the nematophagous fungus Pochonia chlamydosporia and the
nematicide fosthiazate on the multiplication rate of potato cyst nematodes Globodera pallida
and G. rostochiensis. Treatments consisted of an untreated control, P. chlamydosporia, P.
chlamydosporia with the nematicide fosthiazate and fosthiazate on its own. In both
experiments significant reductions in the nematode multiplication rate (Pf/Pi) for P.
chlamydosporia treated plots were observed (48 and 52% control respectively) when
compared with the untreated control. The P. chlamydosporia treatment did not differ
5 th International Congress of Nematology, 2008 294