TABLE CONTENTS

inoculation. The level of nematode biocontrol reached between 55% - 82% in repeated trials.
Surprisingly, the highest level of nematode control was found in fungus wilt resistant
cultivars. Although endophyte colonization decreased over time, the antagonistic fungus
reduced M. incognita in the most of the cultivars tested.
Biodisinfection of Soil as a Means of Alternative Management of Northern
Root-Knot Nematode (Meloidogyne hapla) Damaging Carrot
Douda, O. (1), M. Zouhar (2) & J. Mazáková (2)
(1) Division of Plant Health, Crop Research Institute, Prague, Czech Republic; (2) Department of Plant
Protection, Czech University of Life Sciences, Prague, Czech Republic
Northern Root-Knot Nematode (Meloidogyne hapla) has become a troublesome pest in the
Czech Republic in recent years. Crop failures were reported from root vegetables and
especially from carrot cultivated in Elbe lowland. Chemical treatment is of doubtful efficacy
and currently there are no registered nematocides against this species in the Czech Republic.
Therefore the aim of this research was to evaluate the efficiency of biofumigation on
population density of the Meloidogyne hapla nematode. The infested soil was recovered from
a field with carrot infected with Meloidogyne hapla. The soil was placed in plastic containers;
5 g of cut fresh plant tissue of rapeseed (Brassica napus) was inserted in each container and
containers were covered with transparent polyethylene sheet. After three weeks the sheet was
removed and young carrot plants were replanted in containers. After another six weeks the
plants were examined for the presence of root galls. The statistically significant difference
between experimental variant and untreated and uncovered control variant was determined.
Therefore the amount of the green mass and length of the treatment were proven sufficient to
suppress the nematode population. The research was supported by the Ministry of Agriculture
of the Czech Republic; project number QG 50087.
Evaluation of Biocontrol Efficacy of Pochonia chlamydosporia var.
chlamydosporia Isolates on Heterodera schachtii on Sugar Beet
Fatemy, S.
Nematology Department, Plant Protection Research Institute, P. O. Box 1454-19395, Tehran, Iran.
In Iran, populations of more than 1 egg of sugar beet cyst nematode /g soil cause economic
damage to sugar beet. Environmental concerns regarding nematicides usage have increased
interest in the exploitation of natural biological control agents for nematodes. Strains of
fungus Pochonia chlamydosporia var. chlamydosporia isolated from Heterodera schachtii
have had variable effects on nematode control. An experiment was conducted in which
seedlings of sugar beet were planted in unsterile soil infected with chlamydospores of six
isolates of fungus (10 -3 /g), after a week pots were inoculated with 11 J2 of H. schachtii /g
soil, all pots including control without fungus and five replicates per treatment were kept in a
growth chamber at 25 ○ C with 16 h light. After two months, weight of plant parts were taken
and final nematode and fungal density were determined. Number of cysts, eggs and
reproduction factor were decreased in fungal treated soils; final population of eggs were
reduced by 70%, 60% and 20% in soils treated with isolates 6 and 4, 5 and 3 and 1 and 2
respectively. Reproduction factors were also decreased to less than 1 in pots treated with
isolates 3, 4, 5 and 6.
5 th International Congress of Nematology, 2008 292