TABLE CONTENTS

Interaction between Two Meloidogyne incognita (Tylenchida:
Heteroderidae) Biotypes and the Enthomopathogenic Nematodes
Steinernema feltiae and S. carpocapsae (Rhabditida: Steinernematidae)
Campos-Herrera, R. (1), A. Piedra-Buena (2), S. Labrador (1) & C. Gutiérrez (1)
(1) Dept. Agroecología, Centro de Ciencias Medioambientales, CSIC; (2) Dept. Suelos, Centro de Ciencias
Medioambientales, CSIC;
The effect of entomopathogenic nematodes (EPN), Steinernema feltiae ‘Rioja’ and S.
carpocapsae ‘96’ strains (Rhabditida: Steinernematidae), on Meloidogyne incognita
(Tylenchida: Heteroderidae) BXVIC (virulent) and B66 (avirulent) strains was assessed
under growth chamber conditions using susceptible tomato cv. Marmande (Solanum
lycopersicum, Solanales: Solanaceae). A complete randomized experiment design with 12
replicates/treatments was performed and repeated three repetitions over time. Treatments
were: i) M. incognita avirulent, ii) M. incognita virulent, iii) M. incognita avirulent + S.
feltiae or S. carpocapsae, iv) M. incognita virulent + S. feltiae or S. carpocapsae, v) S. feltiae
or S. carpocapsae, and vi) control treatment without nematode inoculation. Single tomato
plantlets with 2-4 leaves were inoculated with 200 M. incognita infective juveniles (IJ,
treatments i, ii, iii and iv) and 900 IJs of S. feltiae or S. carpocapsae strains (treatments iii, iv
and v). After 60 days, roots were gently rinsed prior to count galls and egg masses. Egg
masses were kept in tap water, and emerging root-knot IJs were counted after 7 days.
Differential effect was observed for nematode interactions. Both EPN species significantly
reduced the number of egg masses and IJs/egg mass when plants were inoculated with the
virulent biotype, with only S. carpocapsae reducing galling. However, only EPN-M.
incognita avirulent biotype interaction with S. carpocapsae, significantly reduced the number
of IJs/egg mass, whereas a significant increase in root galling was observed when either EPN
species was applied, although the number of egg masses/plant was not statistically significant
from the control treatment. Our results suggest that further studies on the EPN-M. incognita
interaction considering different root-knot nematodes biotypes and EPN species are strongly
recommended in order to clarify the possible effects of their simultaneous presence in the
soil.
Influence of Fusarium Wilt Resistant Tomato Cultivars on Root
Colonization of the Mutualistic Endophyte Fusarium oxysporum Strain 162
and as Biological Control of Root-knot Nematode
Dababat, A.E.A., M.E. Selim &R.A. Sikora
Soil-Ecosystem Phytopathology and Nematology, Institute of Crop Science and Resource
Conservation INRES, Department of Plant Health, University of Bonn; Nussallee 9; 53115 Bonn,
Germany
The non-pathogenic endophytic fungus, Fusarium oxysporum Strain 162, originally isolated
from the endorhiza of tomato roots, reduces damage caused by Meloidogyne incognita, by
inhibiting juvenile penetration of and development in the root ability of the nematode
antagonistic. This mutualistic endophyte colonizes the endorhiza of different Fusarium wilt
resistant and susceptible cultivars of tomato (Lycopersicon esculentum) were investigated.
The fungus was colonized the endorhiza of both wilt resistant and susceptible cultivars of
tomato. The colonization percent ranged from 75% - 100% in repeated trials, three weeks
after fungal inoculation. However, percent colonization declined 8 weeks after fungal
5 th International Congress of Nematology, 2008 291